LOCUS       DBEROU010000001       115837 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.001, whole genome shotgun sequence.
ACCESSION   DBEROU010000001 DBEROU010000000
VERSION     DBEROU010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 115837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 115837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..115837
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.001"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..306
                     /locus_tag="ACL8CL_000001"
     CDS             <1..306
                     /locus_tag="ACL8CL_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078495769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS1329331.1"
                     /translation="SASASTSASASASTSASASASTSASASASTSASASASTSVSNSA
                     NHSNSQVGNTSGSTGKSQKELPNTGTESSIGSVLLGVLAAVTGIGLVAKRRKRDEEE"
     gene            367..2451
                     /locus_tag="ACL8CL_000002"
     CDS             367..2451
                     /locus_tag="ACL8CL_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000341103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS1329332.1"
                     /translation="MDDKITVIVPVYNVENYLRKCLDSIISQTYKNIEIVVVNDGSTD
                     ASGEICKEFSEMDHRILYIEQENAGLSAARNTGLNNMSGNYVTFVDSDDWIEQDYVET
                     LYKKIVEYQADIAVGNYYSFNESEGMFYFHISGDYYYEKVYDNVSIFENLYETQEMKS
                     FALISACGKLYKARLFEQLRFDMGKLVEDGYLNQKVYLLSEKVIYLNKGLYAYRIRKG
                     SLSRVWTEKWMHALVDAMSERITLLANMGYPLEKHLAVYRQMLEVSLANGQASGLSDT
                     AIYKEFEMKKRLLNQLTVQESSEKKAIVLAANYGYVDQVLTTIKSICYHNRSIRFYLI
                     HSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKTDISYTVFLRYFIADFVQEDK
                     ALYLDCDLLVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNAFWKK
                     ENMTQKLIDVTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQLPEG
                     QDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIYPIKE
                     PFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNGIHYL
                     VDVDNELVETSQVLLDINHGEKTEEIINQFARLGKTILSFENTKTYEAGQEVYAVDQV
                     QAMIEKLREISK"
     gene            2758..3972
                     /locus_tag="ACL8CL_000003"
     CDS             2758..3972
                     /locus_tag="ACL8CL_000003"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HGS1329333.1"
                     /translation="MNTKSIVFNADNDYVDKLETAIKSICCYNNCLKFYVFNDDIASE
                     WFLMMNKRLKTIQSEIVNVKIVDHVLKKFHLPLKNLSYATFFRYFIPNFVKESRALYL
                     DSDIIVTGSLDYLFDIELDGYALAAVEDSFGDVPSTNFNSGMLLVNVDTWRDEDACSK
                     LLELTNQYHETAYGDQGILNMLFHDRWKRLDRNFNFMVGMDSVAHIEGNHKWYEISEL
                     KNGDLPSVIHYTGVKPWEIISNNRFREVWWFYNLLEWSDILLRKDIISRSFEELVYSP
                     KAHTAIFTASCEMEHVEYLIENLPEVHFSILAHTYFASSVVALLRYSNVTIYPCFSPF
                     DYRKILDNLDFYLDINHYKEVDNIVSVVQQLSKPIFTFENTSHDIGNQTNIFSSTEPN
                     KMVEAIRQFIGE"
     gene            3977..4249
                     /locus_tag="ACL8CL_000004"
     CDS             3977..4249
                     /locus_tag="ACL8CL_000004"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1329334.1"
                     /translation="MADELISIVVPIYNVENYLRMCLDSIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGGRTLLL"
     gene            4216..4854
                     /locus_tag="ACL8CL_000005"
     CDS             4216..4854
                     /locus_tag="ACL8CL_000005"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS1329335.1"
                     /translation="MFGGGAYITFVDSDDWLEHDALDRLYGALKKENADISIGRYNSY
                     DETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRNGNWTVAVLKLFKRELLQDLPF
                     PIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLSDTLSNTWSEKRMYDEIGAREE
                     KIAILASSDYDLTNHILIYKNRLQRVIAKLEEQNMQFTEIYRRMMEKLSLLP"
     gene            4883..5893
                     /locus_tag="ACL8CL_000006"
     CDS             4883..5893
                     /locus_tag="ACL8CL_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS1329336.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYMYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSIEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSSLSTAEFIV
                     DQGLGFVANSLEEVHEIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLFVDAIYHLG
                     VE"
     gene            5903..8347
                     /locus_tag="ACL8CL_000007"
     CDS             5903..8347
                     /locus_tag="ACL8CL_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HGS1329337.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDKVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNAGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDVVALYNNWQEHLAF
                     NDKPAVIHFTTYRKPWTTLIANRYRDLWWEFHDLEWNQILQHHIGEFEMTSPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQVAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERMLADLQKLITKDMLEKPLDIIQVKGIDETLDYIIEHNSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGFESDEKLVVCLPDAFDDRFVFTW
                     WATPFWKEHMNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGITSRSGVGNDLFDKVKSVKRIICPSHNAYSVVDNIQEEIMKHAEGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHVDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            8378..9598
                     /locus_tag="ACL8CL_000008"
     CDS             8378..9598
                     /locus_tag="ACL8CL_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS1329338.1"
                     /translation="MRNTKRAVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            9607..10803
                     /locus_tag="ACL8CL_000009"
     CDS             9607..10803
                     /locus_tag="ACL8CL_000009"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HGS1329339.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKTEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFVTEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNAGMLLMDVVKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNLPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMFVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELISLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            10803..11705
                     /locus_tag="ACL8CL_000010"
     CDS             10803..11705
                     /locus_tag="ACL8CL_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1329340.1"
                     /translation="MSELISVVVPIYNTGKYLVGCVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGAAQAKNMGISVAKGEYITIVDSDDIVKENMIET
                     LYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFNS
                     SAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRSG
                     SIMRTEFDLSWARDIVEVFSKKISDCVLAGLDVSVLRIRFVNLLKDYKQTLEYHQLTD
                     TEEYKDICFRLKLFFDAEQRNGKS"
     gene            11762..12979
                     /gene="secY2"
                     /locus_tag="ACL8CL_000011"
     CDS             11762..12979
                     /gene="secY2"
                     /locus_tag="ACL8CL_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HGS1329341.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLTLMIAVIQSLAVSLRLPVQSSYSAILVVLMNTILLIAGTFFLVWLSDLNASMGI
                     GGSIVILLSSMVLNIPQDVLETFQTVHIPTGIIVLLALLTLVFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMIGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVVMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            12989..14569
                     /gene="asp1"
                     /locus_tag="ACL8CL_000012"
     CDS             12989..14569
                     /gene="asp1"
                     /locus_tag="ACL8CL_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HGS1329342.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHYIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNPQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYFQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNEVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRLQFVNLNDELDLIKTLEFVRLIVDLNRHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLADVTEFSKAAHYYTDRLKEWNESLIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            14553..16088
                     /gene="asp2"
                     /locus_tag="ACL8CL_000013"
     CDS             14553..16088
                     /gene="asp2"
                     /locus_tag="ACL8CL_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HGS1329343.1"
                     /translation="MSKELNILQIGLANWENHYDIPENMSWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYSRDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDDFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            16085..16525
                     /gene="asp3"
                     /locus_tag="ACL8CL_000014"
     CDS             16085..16525
                     /gene="asp3"
                     /locus_tag="ACL8CL_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HGS1329344.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYPDKSVRLYNPLLPSGEI
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     KVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            16518..18890
                     /gene="secA2"
                     /locus_tag="ACL8CL_000015"
     CDS             16518..18890
                     /gene="secA2"
                     /locus_tag="ACL8CL_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HGS1329345.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMVLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKQYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDIVYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            18902..20413
                     /gene="gtfA"
                     /locus_tag="ACL8CL_000016"
     CDS             18902..20413
                     /gene="gtfA"
                     /locus_tag="ACL8CL_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HGS1329346.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNADKVDFFI
                     VSTDRQNEVLQEQFAKYTQHQPKIVTIPVGSIDSLTDSSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLRDIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQENRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            20406..21743
                     /gene="gtfB"
                     /locus_tag="ACL8CL_000017"
     CDS             20406..21743
                     /gene="gtfB"
                     /locus_tag="ACL8CL_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031647 - regulation of protein stability
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HGS1329347.1"
                     /translation="MIELYDRYSHESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGTCFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHYPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKTTYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLCYPNVALY
                     QNASPQKIQELYQLSDIYLDINHSNELLQAVRQAFEHNLLILGFNQTVHNRLYVAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            21745..21924
                     /gene="asp4"
                     /locus_tag="ACL8CL_000018"
     CDS             21745..21924
                     /gene="asp4"
                     /locus_tag="ACL8CL_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HGS1329348.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            21929..22153
                     /locus_tag="ACL8CL_000019"
     CDS             21929..22153
                     /locus_tag="ACL8CL_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HGS1329349.1"
                     /translation="MIDILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLTFMVITYK"
     gene            22214..22366
                     /locus_tag="ACL8CL_000020"
     CDS             22214..22366
                     /locus_tag="ACL8CL_000020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329350.1"
                     /translation="MKIKEQTRKLAAGCSKHGFEVVDRTDEVSSKHGFEVVDRTDEVS
                     NIYTAR"
     gene            complement(22389..23342)
                     /locus_tag="ACL8CL_000021"
     CDS             complement(22389..23342)
                     /locus_tag="ACL8CL_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGS1329351.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(23410..24619)
                     /gene="sstT"
                     /locus_tag="ACL8CL_000022"
                     /pseudo
     CDS             complement(23410..24619)
                     /gene="sstT"
                     /locus_tag="ACL8CL_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            24758..25318
                     /locus_tag="ACL8CL_000023"
     CDS             24758..25318
                     /locus_tag="ACL8CL_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HGS1329352.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQALHFVPNRNITVVSNFSRTD"
     gene            25375..26283
                     /locus_tag="ACL8CL_000024"
     CDS             25375..26283
                     /locus_tag="ACL8CL_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGS1329353.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFA
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            26298..26825
                     /locus_tag="ACL8CL_000025"
     CDS             26298..26825
                     /locus_tag="ACL8CL_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HGS1329354.1"
                     /translation="MAIENYIPDFAVEAVYDLTVPSLQAQGIKAVLVDLDNTLIAWNN
                     PDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVEKFGIDYVYWALKPFTFGIDRA
                     MKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKPLVQHDSIKTQINRTRERRVMR
                     KITEKYGPITYKKGI"
     gene            26828..27934
                     /gene="yqeH"
                     /locus_tag="ACL8CL_000026"
     CDS             26828..27934
                     /gene="yqeH"
                     /locus_tag="ACL8CL_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HGS1329355.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            28149..28460
                     /gene="yhbY"
                     /locus_tag="ACL8CL_000027"
     CDS             28149..28460
                     /gene="yhbY"
                     /locus_tag="ACL8CL_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HGS1329356.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            28512..29141
                     /locus_tag="ACL8CL_000028"
     CDS             28512..29141
                     /locus_tag="ACL8CL_000028"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HGS1329357.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEKNPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRAFLAQGRKPNFLLPQLVLDYIEKEGLY"
     gene            29141..29734
                     /gene="yqeK"
                     /locus_tag="ACL8CL_000029"
     CDS             29141..29734
                     /gene="yqeK"
                     /locus_tag="ACL8CL_000029"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HGS1329358.1"
                     /translation="MSYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDPDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVVYVADYIEHNRSFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKED"
     gene            29735..30235
                     /locus_tag="ACL8CL_000030"
     CDS             29735..30235
                     /locus_tag="ACL8CL_000030"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGS1329359.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLEKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEGWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFDGDDIPAETINEFYEDIWEERFADVL
                     DYKHIF"
     gene            30248..30601
                     /gene="rsfS"
                     /locus_tag="ACL8CL_000031"
     CDS             30248..30601
                     /gene="rsfS"
                     /locus_tag="ACL8CL_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HGS1329360.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            30710..31450
                     /locus_tag="ACL8CL_000032"
     CDS             30710..31450
                     /locus_tag="ACL8CL_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1329361.1"
                     /translation="MATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLELA
                     CGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKIAFIEGNMLNLSKAGKYD
                     FVTCYSDSICYMQDEVEVGDVFKDVYNVLNEEGVFIFDVHSTYQTDEVFPGYSYHENA
                     EDFAMLWDTYEDEVPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQAG
                     FKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            31460..32557
                     /locus_tag="ACL8CL_000033"
     CDS             31460..32557
                     /locus_tag="ACL8CL_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGS1329362.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVIWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILMQARESDLPEAIHVLGFTEKGRQHLKSLKGQVSLVSRIGKEPWDA
                     MTQKADQIYQLGKPSIAQQNFGRVPIRIETN"
     gene            32795..33049
                     /locus_tag="ACL8CL_000034"
     CDS             32795..33049
                     /locus_tag="ACL8CL_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HGS1329363.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFEPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            33053..33307
                     /locus_tag="ACL8CL_000035"
     CDS             33053..33307
                     /locus_tag="ACL8CL_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HGS1329364.1"
                     /translation="MLLKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            33498..35111
                     /locus_tag="ACL8CL_000036"
     CDS             33498..35111
                     /locus_tag="ACL8CL_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HGS1329365.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            35241..35867
                     /gene="gmk"
                     /locus_tag="ACL8CL_000037"
     CDS             35241..35867
                     /gene="gmk"
                     /locus_tag="ACL8CL_000037"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HGS1329366.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            35892..36206
                     /gene="rpoZ"
                     /locus_tag="ACL8CL_000038"
     CDS             35892..36206
                     /gene="rpoZ"
                     /locus_tag="ACL8CL_000038"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HGS1329367.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            36272..38668
                     /locus_tag="ACL8CL_000039"
     CDS             36272..38668
                     /locus_tag="ACL8CL_000039"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HGS1329368.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVFGLESQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLGKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVRRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSHSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNSQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            38681..39616
                     /gene="fmt"
                     /locus_tag="ACL8CL_000040"
     CDS             38681..39616
                     /gene="fmt"
                     /locus_tag="ACL8CL_000040"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HGS1329369.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDNRYEILAVATQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGRDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPEEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSICKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            39609..40922
                     /gene="rsmB"
                     /locus_tag="ACL8CL_000041"
     CDS             39609..40922
                     /gene="rsmB"
                     /locus_tag="ACL8CL_000041"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HGS1329370.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGIEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            40937..41677
                     /locus_tag="ACL8CL_000042"
     CDS             40937..41677
                     /locus_tag="ACL8CL_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HGS1329371.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSMNEEDAE"
     gene            41674..43653
                     /gene="stkP"
                     /locus_tag="ACL8CL_000043"
     CDS             41674..43653
                     /gene="stkP"
                     /locus_tag="ACL8CL_000043"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase StkP"
                     /protein_id="HGS1329372.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSAE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            43768..44526
                     /locus_tag="ACL8CL_000044"
     CDS             43768..44526
                     /locus_tag="ACL8CL_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGS1329373.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            44528..44977
                     /locus_tag="ACL8CL_000045"
     CDS             44528..44977
                     /locus_tag="ACL8CL_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HGS1329374.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            45039..45665
                     /locus_tag="ACL8CL_000046"
                     /pseudo
     CDS             45039..45665
                     /locus_tag="ACL8CL_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            45733..45981
                     /locus_tag="ACL8CL_000047"
     CDS             45733..45981
                     /locus_tag="ACL8CL_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329375.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLDKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            46112..47284
                     /locus_tag="ACL8CL_000048"
     CDS             46112..47284
                     /locus_tag="ACL8CL_000048"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HGS1329376.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTALSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            47284..48558
                     /locus_tag="ACL8CL_000049"
     CDS             47284..48558
                     /locus_tag="ACL8CL_000049"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0015936 - coenzyme A metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HGS1329377.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNDQEYTVPYVTEEPSVVAAASYAIKIIKRTGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLDLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVERLISDKTFNLETAQRYLENLRS"
     gene            complement(48593..49558)
                     /locus_tag="ACL8CL_000050"
     CDS             complement(48593..49558)
                     /locus_tag="ACL8CL_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1329378.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(49539..50993)
                     /locus_tag="ACL8CL_000051"
     CDS             complement(49539..50993)
                     /locus_tag="ACL8CL_000051"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004564 - beta-fructofuranosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HGS1329379.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEHPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            51370..53253
                     /locus_tag="ACL8CL_000052"
     CDS             51370..53253
                     /locus_tag="ACL8CL_000052"
                     /EC_number="2.7.1.211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-specific PTS transporter subunit IIBC"
                     /protein_id="HGS1329380.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSIPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGDAVIE
                     VKI"
     gene            53400..54287
                     /gene="scrK"
                     /locus_tag="ACL8CL_000053"
     CDS             53400..54287
                     /gene="scrK"
                     /locus_tag="ACL8CL_000053"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008865 - fructokinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase ScrK"
                     /protein_id="HGS1329381.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEVVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(54607..55224)
                     /locus_tag="ACL8CL_000054"
     CDS             complement(54607..55224)
                     /locus_tag="ACL8CL_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HGS1329382.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(55639..55852)
                     /locus_tag="ACL8CL_000055"
                     /pseudo
     CDS             complement(55639..55852)
                     /locus_tag="ACL8CL_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(56220..56411)
                     /locus_tag="ACL8CL_000056"
     CDS             complement(56220..56411)
                     /locus_tag="ACL8CL_000056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329383.1"
                     /translation="MKIKEQTRKLAVDCSKYSFEVADKTDEVSSKHCFEVVDRTDEVS
                     NYIYGKAKLTWFEEIFEEY"
     gene            56534..57427
                     /locus_tag="ACL8CL_000057"
     CDS             56534..57427
                     /locus_tag="ACL8CL_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329384.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLADKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            57424..58623
                     /locus_tag="ACL8CL_000058"
     CDS             57424..58623
                     /locus_tag="ACL8CL_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1329385.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGTLNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEA
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILVHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINGYAEGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(58976..59743)
                     /locus_tag="ACL8CL_000059"
     CDS             complement(58976..59743)
                     /locus_tag="ACL8CL_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329386.1"
                     /translation="MFWNLVHYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLAISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAAYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVKELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(59760..60455)
                     /locus_tag="ACL8CL_000060"
     CDS             complement(59760..60455)
                     /locus_tag="ACL8CL_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329387.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDMDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            complement(60460..60825)
                     /locus_tag="ACL8CL_000061"
     CDS             complement(60460..60825)
                     /locus_tag="ACL8CL_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1329388.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            60965..61438
                     /gene="nrdR"
                     /locus_tag="ACL8CL_000062"
     CDS             60965..61438
                     /gene="nrdR"
                     /locus_tag="ACL8CL_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HGS1329389.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            61439..62608
                     /locus_tag="ACL8CL_000063"
     CDS             61439..62608
                     /locus_tag="ACL8CL_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGS1329390.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLVLHAPLSSQD
                     FLEHPVYRRLLEKKIGDVAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLDHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKIQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            62609..63505
                     /gene="dnaI"
                     /locus_tag="ACL8CL_000064"
     CDS             62609..63505
                     /gene="dnaI"
                     /locus_tag="ACL8CL_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HGS1329391.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTVWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            63502..64215
                     /locus_tag="ACL8CL_000065"
     CDS             63502..64215
                     /locus_tag="ACL8CL_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HGS1329392.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYPVFGMAL
                     GVPNQTHDVKPRLPLDNVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLEQKKLL"
     gene            64229..65539
                     /gene="der"
                     /locus_tag="ACL8CL_000066"
     CDS             64229..65539
                     /gene="der"
                     /locus_tag="ACL8CL_000066"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HGS1329393.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            65628..65963
                     /locus_tag="ACL8CL_000067"
     CDS             65628..65963
                     /locus_tag="ACL8CL_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329394.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYIPFY
                     SLFSLAWTVVSVLFFNTVLRNVTDIKLWGIWFLFILLAIGMNWLTYSCFKEMFRLRQE
                     LGKSKGGRH"
     gene            66103..66438
                     /locus_tag="ACL8CL_000068"
     CDS             66103..66438
                     /locus_tag="ACL8CL_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329395.1"
                     /translation="MEHLFKMIILLPCFYFFSWIDKDNRESKFFPIFYYFYWIYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLDYICFKKMLDLRRE
                     LGKSKGGRH"
     gene            66623..67414
                     /locus_tag="ACL8CL_000069"
     CDS             66623..67414
                     /locus_tag="ACL8CL_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329396.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLIIAILTHFFGTFSPLGWNSLFSDGSSLQR
                     LLDGEITSYLFFTCVLLIGIFINAVYFLQIVDYLGNVTRSAITYLMFLWLGSMLLYSA
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYIVSYMVLEKYEDNV"
     gene            67407..68048
                     /locus_tag="ACL8CL_000070"
     CDS             67407..68048
                     /locus_tag="ACL8CL_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329397.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEVNLEIPKLLLNLSLFMVGWLSVILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFFLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLIVSRLEEKFKKG"
     gene            68052..68684
                     /locus_tag="ACL8CL_000071"
     CDS             68052..68684
                     /locus_tag="ACL8CL_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1329398.1"
                     /translation="MRLEIINGQKIYRKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKILAGYIKLDKGKVLQDGKVYGVKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDIEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQM"
     gene            68712..68999
                     /locus_tag="ACL8CL_000072"
     CDS             68712..68999
                     /locus_tag="ACL8CL_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329399.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDKPLLGIRIDIFI
                     IQKILLAFGILSILMALLSKKVSLFVFGLICCLSLWINLFITLAILPIFGN"
     gene            69145..71658
                     /gene="secA"
                     /locus_tag="ACL8CL_000073"
     CDS             69145..71658
                     /gene="secA"
                     /locus_tag="ACL8CL_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HGS1329400.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMTALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            71739..72770
                     /locus_tag="ACL8CL_000074"
     CDS             71739..72770
                     /locus_tag="ACL8CL_000074"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGS1329401.1"
                     /translation="MVFTAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            72772..73803
                     /locus_tag="ACL8CL_000075"
     CDS             72772..73803
                     /locus_tag="ACL8CL_000075"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HGS1329402.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYVTLTK"
     gene            73848..74210
                     /gene="acpS"
                     /locus_tag="ACL8CL_000076"
     CDS             73848..74210
                     /gene="acpS"
                     /locus_tag="ACL8CL_000076"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HGS1329403.1"
                     /translation="MIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLKGR
                     RQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLSIS
                     HTDQFVTASVILEENHES"
     gene            74200..75303
                     /gene="alr"
                     /locus_tag="ACL8CL_000077"
     CDS             74200..75303
                     /gene="alr"
                     /locus_tag="ACL8CL_000077"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HGS1329404.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVRVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPSGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKPYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            75322..77337
                     /gene="recG"
                     /locus_tag="ACL8CL_000078"
     CDS             75322..77337
                     /gene="recG"
                     /locus_tag="ACL8CL_000078"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HGS1329405.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKDLAEYKQALRRIKFEELFYFQMQ
                     LQTLKSENRIQGSGLVLDWSQEKVTAVKASLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQAYVISPLIEESEALDLKNAIALSEELTAHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            77653..78633
                     /locus_tag="ACL8CL_000079"
     CDS             77653..78633
                     /locus_tag="ACL8CL_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HGS1329406.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDAFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAMEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            79483..79710
                     /locus_tag="ACL8CL_000080"
     CDS             79483..79710
                     /locus_tag="ACL8CL_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329407.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTVFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            79998..82895
                     /gene="nanA"
                     /locus_tag="ACL8CL_000081"
     CDS             79998..82895
                     /gene="nanA"
                     /locus_tag="ACL8CL_000081"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HGS1329408.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNNQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYNNYNDAPLKVKPGQWNSVTFTVEKPTAE
                     LPNGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAALTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGNQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLNGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHNGKEYIILSNAGGPNRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKDLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
     gene            83910..84362
                     /locus_tag="ACL8CL_000082"
     CDS             83910..84362
                     /locus_tag="ACL8CL_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS1329409.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGDFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            84381..85718
                     /locus_tag="ACL8CL_000083"
     CDS             84381..85718
                     /locus_tag="ACL8CL_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1329410.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDKDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            85797..86681
                     /locus_tag="ACL8CL_000084"
     CDS             85797..86681
                     /locus_tag="ACL8CL_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1329411.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            86681..87514
                     /locus_tag="ACL8CL_000085"
     CDS             86681..87514
                     /locus_tag="ACL8CL_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1329412.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            87709..88812
                     /locus_tag="ACL8CL_000086"
     CDS             87709..88812
                     /locus_tag="ACL8CL_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGS1329413.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            88986..89684
                     /locus_tag="ACL8CL_000087"
     CDS             88986..89684
                     /locus_tag="ACL8CL_000087"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGS1329414.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            89703..91220
                     /locus_tag="ACL8CL_000088"
     CDS             89703..91220
                     /locus_tag="ACL8CL_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGS1329415.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNTADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            91345..92673
                     /locus_tag="ACL8CL_000089"
     CDS             91345..92673
                     /locus_tag="ACL8CL_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1329416.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            92925..93791
                     /locus_tag="ACL8CL_000090"
     CDS             92925..93791
                     /locus_tag="ACL8CL_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1329417.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            93807..94646
                     /locus_tag="ACL8CL_000091"
     CDS             93807..94646
                     /locus_tag="ACL8CL_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1329418.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            94918..95370
                     /locus_tag="ACL8CL_000092"
     CDS             94918..95370
                     /locus_tag="ACL8CL_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS1329419.1"
                     /translation="MIFDDLKNITFYKGIYPNLDKAIDYLYQHRKDSFELGKYDIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLIEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCYEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            95467..95811
                     /locus_tag="ACL8CL_000093"
     CDS             95467..95811
                     /locus_tag="ACL8CL_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329420.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVNALIGRYIKLGAY
                     AAKYGISMARSILSRAAATAAARVGLLTKIFGWILRIAVLAADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            95821..96015
                     /locus_tag="ACL8CL_000094"
     CDS             95821..96015
                     /locus_tag="ACL8CL_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329421.1"
                     /translation="MKALCIFLLNMLAWTVLAMIIRGGLDGFNEHTWSTILIASLSGI
                     YIDYMSIDKSRKKSKRKKKK"
     gene            96098..96742
                     /locus_tag="ACL8CL_000095"
     CDS             96098..96742
                     /locus_tag="ACL8CL_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGS1329422.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEVKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSVFLLFGFGLLVFIQTGLMEKIFAKYQ"
     gene            96911..97828
                     /locus_tag="ACL8CL_000096"
     CDS             96911..97828
                     /locus_tag="ACL8CL_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HGS1329423.1"
                     /translation="MSDLKKYEGVIPAFYACYDDQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGAKAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTATHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            97846..98730
                     /locus_tag="ACL8CL_000097"
     CDS             97846..98730
                     /locus_tag="ACL8CL_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGS1329424.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(98746..99597)
                     /locus_tag="ACL8CL_000098"
     CDS             complement(98746..99597)
                     /locus_tag="ACL8CL_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGS1329425.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKDSQTYTET
                     VLVATHSQTSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            99797..101854
                     /gene="pbp2b"
                     /locus_tag="ACL8CL_000099"
     CDS             99797..101854
                     /gene="pbp2b"
                     /locus_tag="ACL8CL_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HGS1329426.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKTVEALPSEKRLGSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEINKVNISDSDMSILHQGFYQVAHGTSGLTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            101865..102461
                     /gene="recR"
                     /locus_tag="ACL8CL_000100"
     CDS             101865..102461
                     /gene="recR"
                     /locus_tag="ACL8CL_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HGS1329427.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDSTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            102637..103680
                     /locus_tag="ACL8CL_000101"
     CDS             102637..103680
                     /locus_tag="ACL8CL_000101"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HGS1329428.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVNYDRFTVKTFFISQ
                     SGDSIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALKLAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWENMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            103764..105137
                     /gene="murF"
                     /locus_tag="ACL8CL_000102"
     CDS             103764..105137
                     /gene="murF"
                     /locus_tag="ACL8CL_000102"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HGS1329429.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASGSLLLAP
                     ADPIVEDYLPIDKKVVRFGQGAELEITDLVERKDSLTFKANFLEQALDLPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQHLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGDQSVQLHNQMILSLSPDVLDIVIFYGEDIAQ
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAYDQILLKGSNSMNLAKLVE
                     SLENECK"
     gene            105124..105735
                     /locus_tag="ACL8CL_000103"
     CDS             105124..105735
                     /locus_tag="ACL8CL_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase N-terminal domain-containing
                     protein"
                     /protein_id="HGS1329430.1"
                     /translation="MNASDFAKYLQRMIALTDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQVSDLDADEVAELLKPTSAYATPLMDVRAWIVEDEKICLVRGQGENDWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQGHRGQYLD"
     gene            105826..106530
                     /locus_tag="ACL8CL_000104"
     CDS             105826..106530
                     /locus_tag="ACL8CL_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HGS1329431.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYRENK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            106749..108122
                     /gene="ftsA"
                     /locus_tag="ACL8CL_000105"
     CDS             106749..108122
                     /gene="ftsA"
                     /locus_tag="ACL8CL_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HGS1329432.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKEAFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVPNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVELVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            108139..109398
                     /gene="ftsZ"
                     /locus_tag="ACL8CL_000106"
     CDS             108139..109398
                     /gene="ftsZ"
                     /locus_tag="ACL8CL_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HGS1329433.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            109403..110074
                     /locus_tag="ACL8CL_000107"
     CDS             109403..110074
                     /locus_tag="ACL8CL_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HGS1329434.1"
                     /translation="MNVKENTELVFREVAEASLSANRERGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            110084..110623
                     /locus_tag="ACL8CL_000108"
     CDS             110084..110623
                     /locus_tag="ACL8CL_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HGS1329435.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            110623..110886
                     /locus_tag="ACL8CL_000109"
     CDS             110623..110886
                     /locus_tag="ACL8CL_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HGS1329436.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            110883..111668
                     /locus_tag="ACL8CL_000110"
     CDS             110883..111668
                     /locus_tag="ACL8CL_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HGS1329437.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            111677..112465
                     /gene="divIVA"
                     /locus_tag="ACL8CL_000111"
     CDS             111677..112465
                     /gene="divIVA"
                     /locus_tag="ACL8CL_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HGS1329438.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLVQLAQCMKNQK"
     gene            112813..115605
                     /gene="ileS"
                     /locus_tag="ACL8CL_000112"
     CDS             112813..115605
                     /gene="ileS"
                     /locus_tag="ACL8CL_000112"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HGS1329439.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     EGKPHFVLHDGPPYANGNIHVGHAMNHISKDIIIRSKSMSGFNSPYIPGWDTHGLPIE
                     QVLAKQGVKRKEMDLVEYLKLCREYALSQVYKQRDDFKRLGMSGDWENLYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGETRKFVVAAELLTSLSEK
                     FGWVDVQVLATYRGQELNHIVTEHPWDTAVDELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDAIDKVKFHTEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMTAETIEHVAQLFEVHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVLVNRPNLTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDVVAYDKLRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQVVICDHCASI
                     VEENFVEAVAEGFEEK"
BASE COUNT        35191 a        20759 c        25696 g        34191 t
ORIGIN      
        1 tcagcttcag cgtcaaccag tgcgtcggct tcagcgtcga caagtgcatc ggcttcagca
       61 tcaacgagtg cgtcggcttc agcaagtacc agtgcgtcag cttcagcaag tacatcagtt
      121 tcaaattcag caaaccattc gaactcacaa gttggaaata cttctggatc gacaggtaaa
      181 tcccaaaaag aattgcctaa tacaggtact gagtcgtcaa ttggatctgt gttacttgga
      241 gttctagcag ctgttacagg tattggattg gttgcgaaac gccgtaaacg tgatgaagaa
      301 gagtaagaca acctgtaaag ttaggctaaa ctaactcgcg cacataaatc aaggagaaaa
      361 ttgctagtgg atgataaaat tacggtcatt gtaccagtat acaatgtaga aaactatctg
      421 aggaaatgtc tagatagtat tatttctcaa acatataaaa atattgagat tgttgtcgtt
      481 aatgatggtt ctacggatgc ttcaggtgaa atttgtaaag aattttcaga aatggatcac
      541 cgaattctct atatagaaca agaaaatgct ggtctttctg ccgcacgaaa caccggtctg
      601 aataatatgt ccggaaatta tgtgaccttt gtggactcgg atgattggat tgagcaagat
      661 tatgtagaaa ctctatataa aaaaatagta gagtatcagg ctgatattgc agttgggaat
      721 tactattctt tcaacgaaag tgaaggaatg ttctactttc atatatcagg agactactat
      781 tatgagaagg tatatgacaa tgtttccatc tttgagaact tgtatgaaac tcaagaaatg
      841 aagagttttg ctttgatatc tgcttgtggt aaactctata aggcaagatt gtttgagcag
      901 ttgcgctttg acatgggtaa attagtagaa gatggttacc tcaatcaaaa ggtatattta
      961 ttatcggaaa aggtaattta tttaaataaa ggtctttatg cttatcggat tagaaaaggt
     1021 agtctatcaa gagtttggac agaaaagtgg atgcacgcct tagttgatgc tatgtctgaa
     1081 cgtattacgc tactagctaa tatgggatat cctctagaga aacacttggc agtttatcgt
     1141 cagatgttgg aagtcagtct cgccaacggt caagctagtg gtttatctga cacagcaata
     1201 tataaagagt ttgaaatgaa aaaaagactt ttaaatcagt taacggtaca agaatcaagt
     1261 gaaaagaaag ccattgtcct agcagcaaac tatggctatg tagaccaagt tttaacgaca
     1321 atcaagtcta tttgttatca taatcgttcg attcgttttt atctgattca tagcgatttt
     1381 ccaaatgaat ggattaagca attaaataag cgcttagaga agtttgactc agaaattatt
     1441 aattgtcggg taacttctga gcaaatttca tgttataaaa cggatattag ttacacagtt
     1501 tttttacgct atttcatagc tgatttcgtg caagaagata aggccctcta cttggactgt
     1561 gatttacttg taacgaaaaa tcttgatgat ttgtttgcta cagacttaca agattatcct
     1621 ttggctgctg ttagagattt tgggggcaga gcttattttg gtcaagaaat ctttaatgcc
     1681 ggtgttctct tggtaaacaa tgctttttgg aaaaaagaga atatgaccca aaaattaatt
     1741 gatgtaacca atgaatggca tgataaggtg gatcaggcag atcagagcat cttgaatatg
     1801 ctttttgaac ataaatggtt ggaattggac tttgattata atcatattgt cattcataaa
     1861 cagtttgctg attatcaatt gcctgagggt caggattatc ctgctattat tcactatctt
     1921 tctcatcgga aaccgtggaa agatttggcg gcccaaacct atcgtgaagt ttggtggtac
     1981 tatcatgggc ttgaatggac agaattggga caaaaccatc atttacatcc attacaaaga
     2041 tctcacatct atccaataaa ggaacctttc acttgtctaa tctatactgc ctcagaccat
     2101 attgaacaaa ttgagacatt ggttcaatcc ttgcctgata ttcagtttaa gatagcagct
     2161 agagtaatag ttagtgatcg attggctcag atgacaattt atccaaacgt gactatattt
     2221 aacggaattc actatttggt agatgtcgat aatgaattgg tagaaaccag tcaagtactt
     2281 ttagatatta atcatggtga aaaaacagag gaaattatca atcaatttgc aagacttgga
     2341 aaaactattt tgtcctttga aaataccaaa acctatgaag caggtcagga ggtatatgct
     2401 gttgaccaag ttcaagcaat gattgagaaa ttgagagaaa taagcaaatg aagaaaaatc
     2461 atttagtagg agatgctctg attttgacgg ttagtgatca gattgaagag ttggattatt
     2521 ttttataaaa tttctccgtt catcatatat gaaagttgtt caaacatcag agtgctttat
     2581 aaaatataaa tagacctaaa gatatttaat atgaactgca ccccaaaagt tagacagaaa
     2641 aaatctaact ttttggggtc agtacaatat tagggtgtga ttaattatct ttttaggtga
     2701 aaatgattct atattatagc tgtttgatac gaaatttatt ataaggaaat tatgttaatg
     2761 aatacaaaat ctatagtttt taatgcagat aatgattatg tagataaatt agaaactgca
     2821 attaaatcta tttgttgtta taataattgt ttaaaatttt atgtatttaa tgatgatatt
     2881 gcgtcagagt ggtttttgat gatgaataag cgattgaaga ctatacaatc tgaaatcgtt
     2941 aatgtaaaga ttgtagatca tgttcttaaa aagtttcatt taccgttaaa gaatttaagt
     3001 tatgccactt tctttcgtta ttttatacct aattttgtca aagaaagtcg tgctttatac
     3061 ctagattctg acatcattgt tacaggaagt ttagactatt tatttgatat agaactagat
     3121 ggttatgcct tggcagcagt agaagattct tttggtgatg ttccttctac caattttaac
     3181 tccggaatgt tattagttaa tgtagatact tggagagatg aagatgcttg ttcgaaactg
     3241 ttagaactga ccaatcaata tcatgaaaca gcatatggag atcaaggaat tttaaatatg
     3301 ttattccatg atagatggaa aagattagac cgaaatttta attttatggt ggggatggat
     3361 agcgtcgcac acatagaagg aaatcataaa tggtatgaga tttctgagtt gaaaaatgga
     3421 gatttaccta gtgttataca ttatactggg gtaaaacctt gggaaataat ttccaataat
     3481 cgctttagag aagtttggtg gttttataat ctgttagaat ggtctgatat tttattgaga
     3541 aaagacatta ttagtcgtag tttcgaagaa cttgtataca gtcctaaagc tcatacagca
     3601 atttttacag ctagttgtga gatggagcat gtagaatatt tgatagaaaa tttaccagag
     3661 gtacattttt ctatactagc acatacatat tttgcgtcta gtgtcgttgc tttattaaga
     3721 tatagcaatg ttacgattta tccttgtttt tctccatttg attatcgaaa aattttggat
     3781 aatttagatt tttatttaga tattaatcat tataaagaag tggataatat tgtatccgtt
     3841 gttcaacaac tatctaaacc aatttttacc tttgaaaata ctagtcatga tataggcaat
     3901 caaactaata tattttcttc aaccgaacca aacaaaatgg tagaggctat tagacaattt
     3961 ataggagaat aagtttatgg cagacgaact aattagtatt gtagttccaa tctacaatgt
     4021 tgagaattat ttgcgaatgt gtttggatag cattcagaat cagacgtatc aaaattttga
     4081 gtgtttatta atcaatgatg gctctccaga tcattcatcc aaaatatgtg aagaatttgt
     4141 agagaaagat tctcgtttca aatattttga gaaagcaaac ggcggtcttt catcagctcg
     4201 taacctaggt attgaatgtt cggggggggg gcgtacatta cttttgtaga ctctgatgat
     4261 tggttggaac atgatgcttt agaccgatta tatggtgctt tgaaaaagga aaacgcagat
     4321 attagtatcg ggcgttataa ttcttatgat gaaacacgct atgtgtatat gacttatgtt
     4381 acggatccag atgattctct ggaagtgata gaaggtaaag caatcatgga tagggaaggt
     4441 gtcgaagaag tcagaaatgg gaactggact gtagctgtct tgaagttatt caagagagag
     4501 ttactacaag atttaccatt tcctatagga aaaattgcag aggatactta ctggacatgg
     4561 aaggtacttc taagagcttc gaggatagtc tatttgaatc gttgtgttta ctggtaccgt
     4621 gttggtttat ctgatacttt atcgaataca tggagtgaaa agcgtatgta tgatgaaatt
     4681 ggggctaggg aagaaaagat agctatttta gcaagttcag actatgactt gaccaatcat
     4741 attttgattt ataaaaatag attacaaaga gtgatagcaa aattagaaga acaaaatatg
     4801 cagttcacag agatttatag aagaatgatg gaaaaattgt ctttacttcc gtagatagta
     4861 ataaaaaatg agatagcgta atatgaaatt acatttaaca aatttatacg gcatggctgg
     4921 tgatagtacg gttatcttag ctcaaaatgc tgttcaaaag atagctagtc aactgggatt
     4981 tagagaggtt ggtatttatt tttacaacat tgcttcagat agtccttctg aaatgaataa
     5041 gcgtctggat ggtattatgg ccagtatctc tattggggat attttagtct ttcagtctcc
     5101 aacctggaat ggctttgaat ttgatcgtct cttgtttgac aagctaaagg atatgcaggt
     5161 gaaaattatt tgttttatcc atgatgttgt tcccctcatg tttgatagta actattatct
     5221 catgaaagac tatatgtaca tgtataatct atcagatgtt ttgatagtgc catcagagag
     5281 aatgaaaaca cgcctgatgg aagaaggatt gacgactaag aagattcttg ttcaagggat
     5341 gtgggatcat cctcatgatt tatccttata cacccctgct tttaaaaaag aacttttttt
     5401 tgctggaagt ttagagcgtt ttccagactt acaaaattgg tctcaagata cgcctttgag
     5461 agtattttca aataaagggg aagctagttc tagtgctaga aatctcagca tcgaaggatg
     5521 gaaaaaagat gaggaattgt tgctagaatt atcaaagggt ggatttggcc ttgtctgggg
     5581 aacccatcaa aatgagggag aaagtaacca atactatacc ttgaatatat ctcataaggt
     5641 gagtacctat ctgacagcgg gcatcccagt cattgtacca agtagcttgt caactgctga
     5701 atttatagta gatcaaggct taggctttgt ggcgaatagt ctagaggaag ttcatgagat
     5761 agttgataaa atgaatctac aagaatatca agaaatgacg aatcggataa aaacctttag
     5821 ttatttgcta aaagaaggtt attttactaa aaaattattt gtagatgcaa tttaccattt
     5881 gggagttgaa taagagaaca agatgattaa aacaattgta ttagctggtg atcgtaatta
     5941 catcaggcag ttagaaacaa ccataaaatc tattttatac cacaatcgag atgttaagat
     6001 ttatatttta aatcaagata ttatgccaga ttggtttcgc aaaccacgaa aaatagctcg
     6061 catgttaggt agtgagatta tcgatgttaa actacctgaa caaactgtgt ttcaagattg
     6121 ggaaaagcaa gatcacatta gtagcattac ctatgctaga tattttattg cagattatat
     6181 ccaagaagat aaggttttat atttagacag tgatttgatt gtaaatactt ctttagagaa
     6241 attatttagt atttgtttag aagaaaaatc actcgcagca gttaaagata cagatggaat
     6301 tacatttaat gcaggtgttt tattaatcaa caataaaaaa tggcgtcaag agaaattaaa
     6361 agaacgacta attgaacaga gcattgttac aatgaaggaa gttgaagaag gccgtttcga
     6421 gcattttaat ggtgatcaaa cgatttttaa tcaggtcttg caagatgatt ggttagaact
     6481 aggtagagct tataatttac aagtaggaca tgatgtggtt gctttgtata ataattggca
     6541 ggaacatctg gcttttaacg ataaaccagc ggtgattcat tttacgacct acagaaagcc
     6601 atggactacc ttgatagcca atcgttaccg tgatttatgg tgggaatttc atgatttgga
     6661 gtggaatcaa attttacaac accatatagg agagtttgaa atgacatcgc ctctagataa
     6721 ggaattttct tgtttaactt tgacaaattc ccaagattta gaaggaatag aagagctagt
     6781 tacagctcta cctgaggtgg tatttcatat cgcagcttgg acggatatgg gagataaatt
     6841 aaaaaaatta gctgtatata ataatgtgag attgcatcca caaattgttc ctcctgtatt
     6901 agataagctg aaaaagtcaa caaatctata tttggatatc aatcatggta gtgcagatga
     6961 gaacttttta aaatctttgc aagaacaaga aaaaacgcta ctagcttttc aatcgactca
     7021 gcacggagag ttaggacaaa tcgttttcga aaatgggaaa gtttccttta tgattgatac
     7081 gattaaagat tttaagaaaa acggacatct tacctgtttt cgacaacttc caagtttaac
     7141 ttgtttaacg tttacggctt ctcaggatat cgaacaattg gattacttgg ctggacagtt
     7201 gccaaatgtt gtttttcaag ttgctgcttg gacagctatg gggccaaaat tatatgattt
     7261 gtctaatcgt tatcctaata ttcagctcta tccggcaatt tctagagata agctagacga
     7321 gttgaaggag aagatggatg cttatttaga tatcaaccta ctgacttcaa catccgatat
     7381 cgttgcagaa atggctcatc tatctaaacc tatactagcc ttttataaat ctcaaaatgg
     7441 gaataatggc caaaggttgt attcaagtga acatcctgaa cgaatgttgg ctgatttgca
     7501 aaaattgata actaaggata tgctagaaaa accgcttgat ataatccagg tgaaagggat
     7561 agatgaaacc ttggattata ttattgaaca caactcttct ttagttcgtt ttggagacgg
     7621 agaagtcaat ttgatgtggg gtttgcctat accttatcaa aatcatgatc tagagttagc
     7681 taaccaatta aaacatattg tgggttttga aagtgacgag aaattagtgg tttgtttacc
     7741 tgatgctttt gacgatcgtt ttgtctttac ttggtgggct acaccattct ggaaagaaca
     7801 tatgaatgtt tatatggatt tttataaaga gttgtgtaag ggttcatggt atggctccac
     7861 ctttatctcg cgtccataca ttgattatga agataagagt aaggcaaaag gacaatttga
     7921 aaagctaaag agtatttggg agaataggga tattctgatt gtagagggga ttacatctcg
     7981 ctctggggtt ggaaatgatt tatttgataa ggtcaaatct gtgaaaagga ttatttgtcc
     8041 atctcataat gcttattccg ttgtcgataa cattcaagag gaaattatga aacatgctga
     8101 aggaagactg attttatgta tgctgggtcc tacagcaaaa gttctgagtt ataatctatg
     8161 ccagatgggc tatcaagttt tggatgtagg ccatgttgac tcagagtatg aatggatgaa
     8221 aatgggagct aaaactaagg ttaaattttc tcataaacat actgcagaac ataatttcga
     8281 ccaagatatt gaatttattg atgatgaaac ctataacagt cagattgttg cacgaatatt
     8341 aaactagact atttaaaata aatgataagg atttaaaatg agaaatacca aacgcgctgt
     8401 agtatttgca ggtgattacg cttatattcg acaaatcgaa acggcgatga agtcactctg
     8461 tagacacaat agtcatttga aaatttatct gctaaatcag gacattcctc aggaatggtt
     8521 tagtcaaata agaatatatt tacaagagat ggggggcgac ttgattgact gcaagttaat
     8581 tggctcacag tttcaaatga attggtctaa taaattacct catatcaatc atatgacatt
     8641 tgcacgctat tttattccag attttgtaac agaagataaa gttctctatc tagatagtga
     8701 tttgattgtg actggtgatt tgaccgattt gtttgaatta gacttaggtg aaaattattt
     8761 ggcagcagct cgttcttgct ttggagcagg agtcggcttc aatgctggtg ttctcttgat
     8821 taacaacaaa aaatggggat ctgaaactat tcgacaaaaa ttgattgact taacagaaaa
     8881 agaacatgag aatgtggaag aaggagacca gtcaattttg aatatgttgt ttaaagatca
     8941 atatagttcc cttgaagatc aatataattt tcaaatagga tatgattatg gggcggcagc
     9001 ctttaaacat caattcattt ttgatattcc gctcgaacca ctgccactaa ttttacacta
     9061 tatttctcag gataagcctt ggaatcaatt ttctgttgga cgtctaagag aagtttggtg
     9121 ggaatactct ttgatggatt ggtctgttat tttaaatgaa tggttttcaa agagtgtgaa
     9181 gtaccctagt aaatcacaaa tatttaagtt gcaatgtgtt aatttaacga attcttggtg
     9241 tgtcgagaaa atcgattatt tggcggagca attgccagaa gttcattttc atattgttgc
     9301 ttatacaaat atggcaaatg aactactagc tttaacgcgt tttcctaatg ttaccgtata
     9361 tccaaattcc ttaccaatgt tattggaaca aatagtaata gcttcagatt tgtatttgga
     9421 tttgaatcat gatcgaaaat tagaagatgc atatgagttt gtgcttaagt acaaaaaacc
     9481 aatgatagct ttcgacaata cttgctctga aaatctttct gagatttcat atgaaggtat
     9541 ctatccaagc tccattccga aaaaaatggt tgcagcaatc agatcttaca tgaggtagag
     9601 aacagtatga gaaaatcaat agtattagcg gcagataatg cctatcttat tcctttagag
     9661 acgactataa agtctgtatt gtatcacaat agagatgttg atttttatat tctcaacagt
     9721 gatatagctc ctgaatggtt taaattattg gggagaaaaa cggaagttgt gaattctaca
     9781 attcgcagtg tacacattga taaagaactt tttgaaagct ataaaacagg acctcatata
     9841 aattatgctt cttactttag attttttgtg acagaagtgg ttgaatctga tagggtattg
     9901 tatctggatt ccgatatcat tgtaactggg gaactagcta ctttgtttga gatagatctc
     9961 aaaggatatt caattggtgc tgttgatgat gtctatgcct atgaaggacg aaaatctgga
    10021 tttaatgctg gtatgttact aatggatgtt gtaaaatgga aagaacattc tattgtcaat
    10081 agtttattgg aattagcggc cgagcagaat caagttgttc atcttgggga tcagagtatt
    10141 ttaaatattt attttgagga taattggcta gcgttagata aaacatataa ttatatggtg
    10201 ggtgttgata tttatcacct tgctcaagaa tgtgaacgtc tagatgacaa tctacctaca
    10261 attgttcact atgctagtca tgataaacct tggaatacat atagtatatc tagactacgt
    10321 gaattatggt gggtttatag agatttggat tggtcagaga ttgcttttca acgttccgat
    10381 ttaaattatt ttgaaagaag caatcagtct aaaaaacaag tgatgtttgt gacatggagt
    10441 gcagatataa aacatttaga gtatttagta caacggttac ctgattggca ttttcatttg
    10501 gctgcaccgt gtgattgttc tgaggagctg atctctctat cacagtatac gaatgtaaca
    10561 gtatatcaaa atgtattaca tagtagaatt gattggctat tggacgattc tatagtttat
    10621 ttagatatta atacaggtgg agaggttttt aatgtagtta caagggcaca agaaagtggc
    10681 aagaaaatct tcgcttttga tatcacacgt aaaagtatgg atgatggact ctatgacggt
    10741 attttttctg tggagagacc agatgattta gtggatagaa tgaagaatat agagatagag
    10801 taatgagtga attaattagt gttgtggtac cgatatacaa tacgggaaaa tatttagtgg
    10861 ggtgtgtcga gcatattctg aagcaaacct atcaaaatat agaaattatt ttagttgatg
    10921 acggttctac ggataattct ggggaaattt gtgatgcttt tatgatgcaa gataatcgtg
    10981 tgcgagtatt gcatcaagaa aataaggggg cagcacaagc taaaaatatg gggattagtg
    11041 tagctaaggg agagtacatc acgattgttg attcagatga tatcgtaaaa gaaaatatga
    11101 ttgaaactct ttatcagcaa gtccaagaaa aggatgcaga tgttgttata gggaattact
    11161 ataattatga cgaaagtgac gggaattttt atttttatgt aacagggcaa gatttttgcg
    11221 tcgaagaatt agctatacaa gaaattatga accgtcaagc aggagattgg aaattcaata
    11281 gctcggcctt tatattgccg acatttaagt tgattaaaaa agaattattc aatgaagttc
    11341 acttttcaaa tggtcgccgc tttgatgatg aagcaactat gcatcgcttt tatcttttag
    11401 cctctaaaat cgtctttata aacgataatc tctatctgta tagaagacgt tcaggaagca
    11461 tcatgagaac ggaatttgat ctttcctggg caagagatat tgttgaagtg ttttctaaga
    11521 aaatatcgga ttgtgtcttg gctggtttgg atgtctccgt tctgcgtatt cgatttgtca
    11581 atcttttaaa agattataag caaactttag aataccatca attaacagat actgaggaat
    11641 ataaagatat ttgtttcaga ttaaagttgt tttttgatgc agaacaaaga aatggtaaaa
    11701 gttgaaataa aagaattgtt atttaccata tcacaaacaa tgaaggtgag gggagtgttt
    11761 tatgactaag atttattcgt caatagcagt aaaaaaagga ctatttacct tatttctact
    11821 gtttatctat gtattgggaa gtcgtattac tctccctttt gttgacctaa atactaaaga
    11881 ttttttagga ggttcaacag cctatctagc cttctcagcc gccctaacag gtggaaatct
    11941 aagaagtttg tcaatttttt ctgttggatt gtccccttgg atgtcagcta tgattttatg
    12001 gcagatgttt tctttttcta aacggttggg tttaacatct acgtctatag aaatacaaga
    12061 tcgccgtaaa atgtacctga ccttgatgat agctgtcatt caatcattgg cagttagctt
    12121 gagactgcca gtacaatcct cctattctgc aatattggtt gttctaatga atacaatatt
    12181 gctgatagca ggaacatttt ttcttgtttg gttgtcagat ttaaatgcga gtatggggat
    12241 tggaggttct atagtcattc tcctatccag tatggtttta aatattcctc aggatgtttt
    12301 ggaaacattt cagacagtac acattccaac agggattatt gtgttacttg ctttattaac
    12361 ccttgtcttt tcttatttac ttgcccttat gtatcgagct cgctatttgg ttcctgttaa
    12421 taaaattggc ttacacaatc gatttaaacg ctattcttat ctcgaaatca tgttgaatcc
    12481 tgcaggtggg atgccttata tgtatgtgat gagttttctt agtgtaccag cttatttgtt
    12541 catcttgttg ggatttattt tccctaatca ttcagggtta gcggctttat caaaggaatt
    12601 tatgattgga aagcctttgt gggtctatgt ttatatttcg gtcttatttt tatttagtat
    12661 catttttgct tttgttacga tgaatggaga agagattgca gaccgtatga aaaaatctgg
    12721 agaatacatt tatggtattt atccaggtgc ggatactagt cgatttatta atcgattggt
    12781 ccttcgtttc tcagtcatag gtggtctctt taatgtggtt atggcaggtg gtcccatgct
    12841 ttttgttttg tttgatgaaa agttattacg attggcaatg attccaggtt tgtttatgat
    12901 gtttggaggc atgattttta cgattagaga cgaggtcaag gctttaaggc taaatgagac
    12961 ctatagacct ttgatttagg agacttttat gtattatttt attccagctt ggtatgggtc
    13021 agaaagaaca tggcatgcag atatcactcc atggtatttt tctcattttc gtctagagtt
    13081 tgatgatacc tttcaccaga ttcggctctt tcaagagcaa gatatagatt ctcgtctatt
    13141 agtattagct taccagcctc atctacgtta ttttttatat agacatggtg tgttagaaac
    13201 ggatacttat tccgtttttg atgttatgca agattttcat aatctccata cccaagtttt
    13261 aagcattaga gatattgagt gggatgatga ctgtgaattt atttatagtc cctttacgat
    13321 tatcgttcaa aaaaatggga agaaatttgc taaggttgaa catggagttg aaggcttcat
    13381 cagtgatata cagtattttg gaccgaatgg tcaaatacat atgcactata tcatggatga
    13441 tcgagggttt atatcgagta ttattttttt tgaagatggg caagcagcct atcaagaata
    13501 tttgaatcct aagggagttt ggcaatttag agagcgttta aaagaaggag gacaggtaga
    13561 agtcaatcca attttgggtt atcgctttaa aatgcttacc tatcaaaata tgggagatct
    13621 ggtggcagaa ttttttgaga attatctgca aacgtatgtg aaggatcagg atatttttat
    13681 gcttccttct cattctcatc atgaccagtt ggtactagat cgtttaccta gtactaatcc
    13741 taaactgttg agtctgttca ttggacgtaa tcctcaagat acctttaggg atttagatgt
    13801 aacttttgaa aaatcggatt tgattttggt ggatagagag gatagtttac gattgttgca
    13861 ggagttgtat cctgaacgaa tgcatcaatg ttatcattta tcatcttttg acacccgatt
    13921 acgattggga cgaagccaaa ctaagaaaga atccatcatt tattttcaac tggattttga
    13981 gcaggggatt gataatcaag ctctgcttca agtcttgtcc tttgtcgctg aaaataagga
    14041 tactgaggtg atttttggag cctttgctgc tagtcaggag caaatgaatg aggttgaagg
    14101 gattgttgag tctttcatcc aagaaaacat tcaatccgaa aatctgggaa aggcgattga
    14161 ttatggtgat gcagaaaatc ctctggaaga aaatcaacac caggacttac gcttacagtt
    14221 tgttaacttg aatgatgagt tagatttgat aaaaacacta gaatttgtcc gtttgattgt
    14281 ggatttaaat agacatcctc atctctacac acagattgct gggattagtg caggaattcc
    14341 tcaaatcaac ctagttgaaa ccgtctatgt tgaacattta aaaaatggtt atttgttagc
    14401 agatgttaca gaattttcta aggctgcaca ttattacaca gataggttga aggagtggaa
    14461 tgagtccttg atatattcaa ttgataagat taaggagcac acaggacaac aatttcttgg
    14521 aaaattagag aaatggatag aggaggttaa aaatgtcaaa ggaacttaat attttacaga
    14581 taggacttgc caattgggaa aatcactatg acatacctga aaatatgagt tggtattatt
    14641 tttacccaaa ctcatcaaaa gcccttcgtg aaataattga aaaagaggat attaaccgtt
    14701 ttcatgcagt tttaatagaa gatggtcagt attccagaga cttattttcc tatgtaaaat
    14761 attttgaacc ttatactttg ttctataacc agaatctaca aataaatgat agagaggttg
    14821 tggattttct aaaaaaacga tgtgcacaag caattgattt tttaagtccc caacaactaa
    14881 tcaatgattt aagtaaatct ctttttggcg gtgggtatgg tgataaactc tttcctccga
    14941 cgatacaagt caatccaaat tttacaggag ctatttctta tcaaggattg gattatgtca
    15001 gtttggaagg tgagtttggg caagattttg cccagcttgc ctattgggct tataatatta
    15061 tggtgcaaaa aacactccct attgagttgt ggcttgaata tgagaaggaa ggcaattgtg
    15121 actttcgttt ggttatccgt aaaatgtgga gtgggtctgt tgatgatttc tttgaagaag
    15181 taatagtatc tgaaaaagac ttggagcaag cactttttat ggatagtcga gacggagact
    15241 actttctctc gatatctgtt gaagcaagag gtcgtggaac tatcaaacta ggtaatcttc
    15301 atcaacgatg gagtcgaaaa caatttggta agtttgtact tggtggaaat atcctacatg
    15361 attccaagcg tgatgaaata aactatttct tccatccagg tgattttaaa ccgcctttga
    15421 ctgtctattt tgcaggttat cgacctgcag aaggattcga gggttacttt atgatgaaaa
    15481 ctcttggatg tcccttcatt ttattttctg atccacgttt agagggggga gctttttatc
    15541 tcggaacgga tgagctagag ggaaaagtaa aggatacgat cactcactat cttgattatt
    15601 taggctttga tcataaggat ttgattttat caggtctttc tatgggaacg tttccggctc
    15661 tctattatgg tgcttctttt gaaccccatg ccatcatagt tggtaagccc ttggctaatt
    15721 taggaactat agctagtcgt ggacgtttgg acgcaccggg tgtctctaac ttagcttttg
    15781 attgtttaat tcatcataca ggtgggacaa gttctcaaga tatgacggag ttggatcagc
    15841 gtttttggaa aatttttaaa caagcaaatt tttcaaagac aacctttggt ttatcctata
    15901 tgaaagatga agaaatggat ccacaagcct atgaacaatt agtgtcttat ctgtgtaata
    15961 caggtgcgaa gattttatct aaaggaactg ctggacgaca caatgatgat acagatacca
    16021 atatttcttg gtttttgcac ttttatagaa tggtcttaga gactggtttt ggaagggaga
    16081 aaagatgatt attacacaga gacagtctat ttattgggga gaagttggtg ggacttatat
    16141 gtatggaaca actgtatctt attaccctga caaaagtgtt cgtctgtata atcctctatt
    16201 gccatctggt gagattctaa agacttggtt ttctagtgtc aattaccagg ctgcacgaac
    16261 ccaacctcag cttcccttat taaaaagaaa gcaggagtat caactatcac tggtttttga
    16321 ttgccagcct gaaaatggag tttataccaa gataactttt tttgaccgct atggtgatat
    16381 tttagaaaaa aaggtagaaa aagcgaaaga tttcatattt acttatccag aagatagtta
    16441 tacttatcaa gtttctcttt taagtgctgg atttgagtcc ttaacttttt atcatttttc
    16501 tatcaaggag atcagaagtg tttagacgtt taggtcaaga tttccagctt aggaaagtga
    16561 aaaagatttt aaagcagatt aatgccctga aaggcaagat gtcctctctt tcggatcaag
    16621 aattagtagc taaaacagta gagtttcgtc agcgtctttc cgagggagaa agtctagacg
    16681 atattttggt tgaagctttt gctgtggtac gtgaagcaga taagcggatt ttagggatgt
    16741 tcccctatga tgttcaagtc atgggagcta ttgtcatgca ctatggaaat gttgctgaga
    16801 tgaatacggg ggaaggtaag accttgacag ctaccatgcc tgtctatttg aacgcttttt
    16861 caggagaagg agtgatggtt gtgactccta atgagtattt atcaaagcgt gatgccgagg
    16921 aaatgggcca agtttatcgt tttctaggat tgaccattgg tgtaccattt acggaagatc
    16981 caaagaagga aatgaaagct gaagaaaaga agcttatcta tgcttcggat atcatctaca
    17041 caaccaatag taatttaggt tttgattatc taaatgataa cctagcctcg aatgaagaag
    17101 gtaagttttt acgaccgttt aactatgtga ttattgatga aattgatgat atcttgcttg
    17161 atagtgcaca aactcctctg attattgcgg gttctcctcg tgttcagtct aattactatg
    17221 cgatcattga tacacttgta acaaccttgg tcgaaggaga ggattatatc tttaaagagg
    17281 agaaagagga ggtttggctc actactaagg gggccaagtc tgctgagaat ttcctaggga
    17341 ttgataattt atacaaggaa gagcatgcgt cttttgctcg tcatttggtt tatgcgattc
    17401 gagctcataa gctctttact aaagataagg actatatcat tcgtggaaat gagatggtac
    17461 tggttgataa gggaacaggg cgtctaatgg aaatgactaa acttcaagga ggtctccatc
    17521 aggctattga agccaaggaa catgtcaaat tatctcctga gacgcgggct atggcctcga
    17581 tcacctatca gagtcttttt aagatgttta ataagatatc tggtatgaca gggacaggta
    17641 aggtcgcgga aaaagagttt attgaaactt acaatatgtc tgtagtacgc attccaacca
    17701 atcgtccgag acaacggatt gactatccag ataatctata tatcacttta cctgaaaaag
    17761 tgtatgcatc cttggagtac atcaagcaat accatgctaa gggaaatccc ttactcgttt
    17821 ttgtaggctc agttgaaatg tctcaactct attcgtctct cttgtttcgt gaagggattg
    17881 cccataatgt cctaaatgct aataatgcgg cgcgtgaggc tcagattatc tccgagtcag
    17941 gtcagatggg ggctgtgaca gtggctacct ctatggcagg acgtggtacg gatatcaagc
    18001 ttggtaaagg agtcgcagag cttgggggct tgattgttat tgggactgag cggatggaaa
    18061 gtcagcggat cgacctacaa attcgtggcc gttctggtcg tcagggagat cctggtatga
    18121 gtaaattttt tgtatcctta gaggatgatg ttatcaagaa atttggtcca tcttgggtgc
    18181 ataaaaagta caaagactat caggttcaag atatgactca accggaagta ttgaaaggtc
    18241 gtaaataccg gaaactagtc gaaaaggctc agcatgccag tgatagtgct ggacgttcag
    18301 cacgtcgtca gactctggag tatgctgaaa gtatgaatat acaacgggat atagtctata
    18361 aagagagaaa tcgtctaata gatggttctc gtgacttaga ggatgttgtt gtggatatca
    18421 ttgagagata tacagaagag gtagcggctg atcactatgc tagtcgtgaa ttattgtttc
    18481 actttattgt gaccaatatt agttttcatg ttaaagaggt tccagattat atagatgtaa
    18541 ctgacaaaac tgcagttcgt agctttatga agcaggtgat tgataaagaa ctttctgaaa
    18601 agaaagaatt acttaatcaa catgacttat atgaacagtt tttacgactt tcactgctta
    18661 aagccattga tgacaactgg gtagagcagg tagactatct acaacagcta tccatggcta
    18721 tcggtggtca atctgctagt cagaaaaatc caatcgtaga gtactatcaa gaagcctacg
    18781 cgggctttga agctatgaaa gaacaaatcc gtgcggatat ggtgcgtaat ctcctgatgg
    18841 ggctggttga ggtcactcca aaaggtgaaa tcgtgactca ttttccataa gaggagaaaa
    18901 tatgacaatt tacaatataa atttaggaat tggttgggct agtagcggtg ttgaatacgc
    18961 tcaagcctat cgtgctggtg tttttcggaa attaaatctg tcctctaagt ttatctttac
    19021 agatatgatt ttagccgata atattcagca cttaacagcc aatattggtt ttgatgataa
    19081 tcaggttatc tggctttata atcatttcac agatatcaaa attgcaccta ctagcgtgac
    19141 agtggatgat gtcttggctt actttggtgg tgaagaaagt cacagagaaa aaaatggcaa
    19201 ggttttacgt gtattctttt ttgaccaaga taagtttgta acctgttatt tggttgatga
    19261 gaacaaggac ttggttcaac atgccgagta tgtttttaag ggaaacctga ttcggaagga
    19321 ttacttttct tatacgcgtt attgtagcga gtattttgct cccaaggaca atgttgcagt
    19381 cttataccaa cgaacttttt ataatgaaga cgggactcca gtctatgata tcttgatgaa
    19441 tcaagggaag gaagaagttt atcatttcaa ggataagatt ttctatggaa agcaagcttt
    19501 tgtgcgtgcc tttatgaaat ctttgaattt gaataagtct gatttggtca ttctcgatag
    19561 ggagacaggc attggtcagg ttgtgtttga ggaagcacag acagcacatt tagcggtagt
    19621 tgttcatgcg gagcattata gtgaaaatgc tacaaatgag gactacatcc tttggaataa
    19681 ctattatgac tatcagttta ccaatgcaga taaggttgac ttctttatcg tgtctactga
    19741 tagacaaaat gaagttctac aagagcaatt tgccaaatat actcagcatc agccaaagat
    19801 tgttaccatt cctgtaggca gtattgattc cttgacagat tcaagtcaag ggcgcaaacc
    19861 attttcattg attacggctt cacgtcttgc caaagaaaag cacattgatt ggcttgtgaa
    19921 agctgtgatt gaagctcata aggagttacc ggaactaacc tttgatatct atggtagtgg
    19981 tggagaagat tctctgctta gagacattat tgcaaatcat caggcagagg actatatcca
    20041 actcaagggg catgcggaac tttcgcagat ttatagccag tatgaggtct acttaacggc
    20101 ttctaccagc gaaggatttg gtctgacctt gatggaagct attggttcag gtctacctct
    20161 aattggtttt gatgtgcctt atggtaatca gacctttata gaggatgggc aaaatggtta
    20221 tttgattcca agttcatctg accatgtaga agaccaaatc aagcaagctt atgccgctaa
    20281 gatttgtcaa ttgtatcaag aaaatcgttt ggaagctatg cgtgcctatt cttaccaaat
    20341 tgcagaaggc ttcttgacca aagaaatttt agaaaagtgg aagaaaacag tagaggaggt
    20401 gctccatgat tgaactttat gatcgttata gtcatgaaag tcgagattta catgaaagtc
    20461 tagtcgctac tggtctttct caacttggag tggtcatcga tgcagatggt tttctgcctg
    20521 atggtctgct ttctcctttt acctattatc taggttacga ggatggaaaa cctctctatt
    20581 ttaatcaagt tcccgtttca gatttttggg aaattttagg agataatcag tctgcttgta
    20641 ttgaagatgt gacgcaggag agggctgtca ttcattatgc tgatggaatg caggctcgct
    20701 tggttaaaca ggtagactgg aaagacctag aaggtcgagt acgtcaggtt gaccactata
    20761 atcgtttcgg aacttgtttt gctaaaacca cttatagcgc agatagcgag ccgattatga
    20821 cagtttacca agatgtcaat ggtcaacaag ttttactgga aaaccatgtg acgggtgata
    20881 tcttattgac tttgcctggt cagtccatgc gttactttgc aaataaagtt gaatttatca
    20941 ccttcttttt gcaagatttg gaaatagaca ccagtcagct tatctttaat actctagcga
    21001 ctcctttctt ggtttccttt cattatccag ataaatctgg ctcggatgtc ttggtatggc
    21061 aggaacctct ttatgatgcc attccaggta atatgcagtt gattttggaa agtgataatg
    21121 tgcgtactaa gaagatcatc attccaaata agacgactta tgagcgcgct ttagagttaa
    21181 ctgacgagaa ataccatgat cagtttgtgc acttgggtta tcattaccag ttcaaacgtg
    21241 ataatttcct aagacgagac gccttaatct tgaccaattc agatcagatt gagcaagtag
    21301 aagcaatcgt agaagccttg cctgatgtta ccttccgtat cgcagcagtg acagagatgt
    21361 cttctaagct cttagacatg ctttgctatc ctaatgtggc cctttaccag aatgctagtc
    21421 cacagaagat tcaggagctg tatcaactgt cggatattta cttggatata aaccacagta
    21481 atgagttgct acaggcagtg cgtcaggcct ttgagcacaa tctcttgatt cttggcttta
    21541 atcagacggt gcacaataga ctttatgtcg ctccagacca tctatttgaa agtagtgaag
    21601 ttgctgcttt ggttgagacc attaaattgg ccctttcaga tgttgatcaa atgcgtcagg
    21661 cacttggcaa acaaggccaa catgcaaatt atgttgactt ggtgagatat caggaaacca
    21721 tgcaaactgt tttaggaggc taacatgtca gaggaagatt tattttacaa agacgttgaa
    21781 ggccgcatgg aagagttgaa acaaaaaccc atcaagaagg aaaaagaaac ccgaggggaa
    21841 aagattagta agactttttc acttttactg ggtttgatga ttctgattgg tttgctcttt
    21901 actttgctgg gaattttgag gtagatctat gattgacata ctaattgttt tagctattat
    21961 cctatctctt gctttgattg tattggtaac tatacaaccc cgtcaaaatc aactattttc
    22021 catggatgcc actagtaata ttggtaaacc aagctactgg cagagcaaca ccttggtcaa
    22081 ggtgctcact ttattggtga gtttggcttt atttgttcta ctattaacct ttatggtgat
    22141 tacttataaa taaaagaaaa cttcagatat tcaccttttg tggattggtc tgaagttttc
    22201 ttttttatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcag gctgctcaaa
    22261 acacggtttt gaggttgtgg atagaactga cgaagtcagc tcaaaacacg gttttgaggt
    22321 tgtggataga actgacgaag tcagtaacat atatacggca aggtgaagct gacgtagttt
    22381 gaagagtatt actgtctata tttttggtaa aaatcaactt ttacttggat gaaggttttg
    22441 gcttcacgta ggagttgaag aagggtggcg cgggtttcaa attcttctct tgtcttgggc
    22501 agactgcggt tccggaagac ttccagataa cgttcaattt catctagcaa atcagaagca
    22561 ggattggtct ggctcagttg acctgcaatt tttgaaaaga gttgcgctaa gatcaggctt
    22621 tcactggcgg caaggtgaca agtgttaatc tgttgggcca tgtttctcag gatacgactt
    22681 tgtcgctgtc tcatctcaaa gtagtggata tggtagtcgg tctggtgaaa gaggtggtca
    22741 gagtgatcca aatagaccag tctgagggct tctttcaaaa gcgtgtctaa ttctgctacc
    22801 agctgtgctc ggttgcgtcc gtctcctctg gataaatagt atttgaagcg ctggaggata
    22861 tcttttaact tttcttccac cagcgtgtgg tagtgctgga tttcctcttc tcgtgaaggc
    22921 atatagagat taacaagcaa ggcaaatcct gtaccaatag caaagagaag gaattcgttg
    22981 actaggaggt ctggagaggt tgactcttga accaagagat ggctaaccaa aaccgtgctt
    23041 ggtgtgatgc caatttccca gcccatcttg taggctaaag gaacgtagaa ggccagatag
    23101 aggccgagac tccagatatg aaatccgctc aagtgaaaag ctagaacacc gatagccaga
    23161 gctagaagca tagaaaaaag acgattgcga gccagtttta aagtacttct acgcgtatca
    23221 gataggctca agagagcgat aattccagcc gaaactgctg acgaaagatt gagaaaataa
    23281 gcaagcaggc aggcaagaca ggtagctaag atgagcttgg tcgtacgttg gctaatagac
    23341 ataagaattt cctaataagt tagaataaaa gcgtaaaaga caagacatga gcaggcttgc
    23401 cttgatgagt tattttttac gggttgctgc gtattcggca acggcggtaa agaggacatc
    23461 tgtagaagag ttaagggctg tttcacatga gtcttggatg acaccaatca caaaaccaac
    23521 cccaacaatt tgtatggcaa tatcgttaga aataccgaaa aggctacaag caactgggat
    23581 aagaaggagg gaacctccgg caatacctga agcaccacag gctgagatag ctgctaccac
    23641 actgaggaca aaggctgtgg caaagtcaac aggaattcca agagtgttaa ctgtaacaag
    23701 ggtcaaaagg ttaatggtaa tcgctactcc agccatattg atagtagaac cgagtgggat
    23761 agaaacagaa taggtatctg ggttgagtcc aaggtcatgg cagagtttca tattgacagg
    23821 aatgttagcc gcagaactac gagtgaaaaa ggctgtcaca ccgctgacac ggaggcagtt
    23881 ccaaactaga gggtaaggat tgcgtctcat aaagaagaag gcaatcaaag ggttgaccac
    23941 aggggcaaca aaaagcatag tcgttactaa tagaaccaat aaaataccgt agttggcaag
    24001 gcttccgact cccttgtcag aaatggtttt aaaaacaaga ccaaggattc caaatggagt
    24061 cagattgatg atccattcga caagtttaga agtcacgtca gcgatagttt ttagcaattc
    24121 tttactattt ttactggctt ctctcatagc gattccaaaa atgactgccc aagataagat
    24181 tccaatatag ttagcagtaa gcagggcgtt gactgggctg tcaaccagtt tgagcaagag
    24241 gttgctgagg acctgcccaa tcccatctgg tggtgcaatt tcagtattgg cactatttag
    24301 ggtaatttca atagggacga tgaaacttgc tagtacagct acaagagcag cggcgaaagt
    24361 ccctatcata ggatatacaa gaaaacaaca gttttcatat tgctatcttg tcccttttga
    24421 tgttgggaaa gggcattggc aacgagggca aagactagga taggagcaac agcttttaga
    24481 cctccaacga ataaatcccc gagtagccca atccctgaga gattaggaag ggtcagtcct
    24541 aggattcccc ataaagcata ccaatcaaga tacgcttgac aaggcttgcc ttattccaag
    24601 catgaatgat tcttttcata ataatctcct ttttgtgtag tgattatgat tatagtataa
    24661 atgatagaca aaatcaagaa ttttctgtct attttttgaa tatgtatgga gaatgagact
    24721 gatgaaaata tggtataatg aaataaagga gttttatatg caaaaattta ttcaggctta
    24781 tattgaaaag ctagatgtga caaccattat cgagaatatt ctaaccaagg tcatttctct
    24841 tttactgctt ttgattgtat tttatattgc taaaaaaatg cttcatacca tggtgcagag
    24901 aattgtcaaa ccttctctaa aaatgtctcg tcatgatgtc ggacgccaaa aaaccatctc
    24961 acgtttacta gaaaatgtgt ttaattatac gctatatttc tttttactct actgcatttt
    25021 gtcgatttta ggtttgccag tttctagttt gctggctgga gctggtattg ctggggtagc
    25081 gattggtatg ggagcccaag gctttctatc tgatgtcatc aatggctttt tcatcctctt
    25141 tgaacgtcaa ctggatgtgg gagatgaggt cgttctgaca aatggaccga ttactgtatc
    25201 gggtaaggtt gtcagtgtgg gaattcgtac gacacagctt cgtagcgagg agcaagccct
    25261 tcactttgtc cctaaccgaa atatcacagt tgttagcaat ttctcacgca cagactagac
    25321 ctgttatttt aagtaatttg tggtacaata gagggagttt aataaggaga aaagatggtt
    25381 ttagaaaagc agttgggcaa tggttgtacc tggatagacc tagacctagg aaagttgaat
    25441 aaactagaag acctttctga aatttacggt ttggacaagg aaaccattga atacgcactg
    25501 gatagaaacg agcgcgccca catggactac caccgtgaaa gtgggacggt tacctttatc
    25561 tataatgtcc tagacgtaaa aaaggacaag gcctactatg agacttttcc catgaccttt
    25621 atcgtcgagc atcgtcgcct gattaccatt agtaatacca agaacgccta tgtcattgaa
    25681 cagatgactc gttatctgga gaaccatgac acgctttcga tttataagtt tctctttgcc
    25741 agtctggaaa tcatcagcaa tgcctactat cctgtcattg agcagatgga caagagtagg
    25801 gatgaggtca atgacctctt gcgccagcga actaccaaga aaaacctctt tgccctgtct
    25861 gatttggaga ctggtatggt ttatctgacg gcagctgcca aacaaaatcg gattttgtta
    25921 gagcatattc aaggtcatgc cttgtatcgt agttttgatg agattgagag agaacagttt
    25981 gatgatgcca tgattgaggc tcatcagctg gtatccatga cagacctaat ctctcagatt
    26041 ttacagcagc tttcagcctc ttacaacaat attctaaaca ataatctgaa tgacaatttg
    26101 acaaccttga ctattatttc agtcttgcta gctgttttgg cagtcgtgac aggctttttc
    26161 ggaatgaatg ttcccttacc tttaacagat gagccccatg cttggctcta tattagtttg
    26221 gctagtgcag gtttgtggat tgttttatcc ttgttactaa ggaaaattgc gaaaaaaagt
    26281 taagaaaagg agccagaatg gcgattgaaa attatatacc agattttgct gtggaagcag
    26341 tctatgatct gacagtccca agcctgcagg cgcagggaat caaggctgtt ttggtcgatt
    26401 tggataatac cctcattgct tggaacaacc ctgatggaac gccagagatg aagcaatggc
    26461 tacatgacct tcgggacgcg ggtattggca ttatcgtagt gtcaaataac accaaaaaac
    26521 gcgttcaacg agcagttgag aaatttggga ttgattacgt ttactgggcc ttgaagccct
    26581 tcacatttgg tattgaccgt gctatgaagg aattccacta tgacaaaaag gaagtggtca
    26641 tggttggtga ccagctcatg acagatatac gagcagccca ccgtgcaggg attcggtcaa
    26701 ttttagtcaa acccttggtc caacatgact caatcaaaac gcagattaac cgaactcgtg
    26761 agcgtcgtgt tatgagaaaa atcactgaaa agtacggacc gattacatat aaaaaaggaa
    26821 tttaactatg gaagaaattc tctgtattgg ttgtggagca accattcaga cgacagataa
    26881 ggctggtctt ggttttaccc cccagtcggc acttgaaaaa ggtttggaga ctggcgaagt
    26941 ctattgccaa cgctgtttcc gtctccgcca ctacaatgaa atcacagatg tccagttgac
    27001 gaacgatgat ttcctcaagc tcttgcacga ggtgggagac agtgatgcct tagtggtcaa
    27061 tgtcattgat atctttgatt ttaatggctc tgtcatccca ggtttaccac gtttcgtctc
    27121 gggcaatgat gtcctcttgg taggaaataa aaaagatatc ctccctaagt cagttaagtc
    27181 tggtaagatt agccagtggc tcatgaaacg tgcccatgaa gaaggtcttc gtccagtcga
    27241 tgtggtccta acttcagcac aaaataaaca tgccattaag gaagtcattg acaagattga
    27301 acactaccgt aagggccgcg atgtctatgt ggtcggtgtg accaacgttg gaaaatcaac
    27361 tctaatcaat gctattatcc aagaaatcac gggtgatcag aatgtcatca ctacttcacg
    27421 cttcccaggg acaaccttgg acaaaataga gattccgctt gacgacggat cttatattta
    27481 cgatacgccg ggaattatcc accgccacca gatggctcac tacttgacgg ccaaaaacct
    27541 caagtatgtc agtcctaaaa aggaaatcaa gcctaagacc tatcagctta atcctgagca
    27601 aaccctattt ttaggtggtt tgggacgctt tgactttata gcaggagaaa agcaaggatt
    27661 tactgctttc tttgataatg aactcaaact ccatcgtagc aagcttgaag gagctagtgc
    27721 tttctacgat aagcacctgg gaactcttct gacaccacca aatagcaagg aaaaagaaga
    27781 tttcccaagg ctagtccagc atgtctttac cattaaagat aagacagacc tagtcatctc
    27841 aggcctaggc tggattcgtg taacaggcat agcaaaagtc gccgtctggg caccagaagg
    27901 cgtcgccgtc gtcacacgaa aagcaattat ttaagcacag aaaggaaagg gttgtctgaa
    27961 tttgggcgag caaggcgagc cccatagaga atacttttcg ctgtggtgta agttggtaca
    28021 agtgattgta ccaactgcgg aaaatttgag accttaggct caaattttag tcatgaaagt
    28081 ccgaaggact ttgctgacgt ccgtcaccac ttcagaaaag tataaaaaga aactctttta
    28141 aagaaattat gtcattaaca tcaaaacaac gtgccttcct caacagccag gcacacaccc
    28201 tcaaacctat catccaaatc gggaaaaatg gactcaacga ccaaatcaaa accagcgtcc
    28261 gtcaagctct tgatgcgcgt gaattaatca aggttactct cttacaaaac acagatgaaa
    28321 acatccacga agtagctgaa attttggaag aagaaatcgg tgtggataca gtccaaaaaa
    28381 taggacgcat cttgattttg tttaaacaat ctagcaagaa agaaaatcgc aagatttcta
    28441 agaaagtcaa agaaatctaa gaccgaaact ccaaataact gtttttatag agaaataaag
    28501 gggactagcc tatgacaatc gaactattga ctccctttac caaggtagag ttggagccag
    28561 aaatcaagga gaaaaaacgc aaacaagttg ggattttagg ggggaatttt aaccctgttc
    28621 acaatgccca tctcattgtt gcggatcaag tacggcaaca gttgggactg gatcaagttc
    28681 tgctcatgcc tgaataccaa cctcctcacg ttgataaaaa ggaaaccatc cctgaacacc
    28741 atcgtctcaa gatgcttgag ttggcaattg agggaattga cggcctagtc attgaaacca
    28801 ttgagttgga gcgcaagggt atttcctaca cctacgacac catgaagatt ttgacagaga
    28861 agaatccaga tacggattat tactttatca tcggtgccga catggttgac tatctgccta
    28921 agtggtaccg aattgatgaa ctggttgaca tggttcagtt tgtgggggtt cagcgtccac
    28981 gctacaaggc agggacttcc tatccagtta tctgggtgga cgtaccgctc atggatatct
    29041 cgtccagcat ggtgcgtgcc ttccttgccc aaggtcggaa acccaacttt ctcctacctc
    29101 agcttgtgct agactatatc gagaaggagg ggctctactg atgtcctatc aagactatat
    29161 caactgctct cgtgaggcct tgttggaaaa aatggcagag cttctacctg agaaacgtct
    29221 aacccattgt ctaggtgtgg agcgtgccgc tatggaatta gctcagcgct ttggtgtaga
    29281 tgttgagaaa gcaagtctag caggtcttct tcatgactat gctaaaaagt tgtcagatca
    29341 ggaatttcta gacttgattg accgttacca gctagaccct gacctcaaaa actggggcaa
    29401 taatgtctgg catggtatgg tcggaattta caaaattcag gaagatttgg atttgcatga
    29461 ttcagaaatc ctgcgagcca ttgaagtcca tacagtcggt gctggtcaga tgacagacct
    29521 agataaggtt gtctatgtag cagactatat cgagcacaat cgatcctttc ctggagtaga
    29581 tgtggcgcgt gagattgcaa gtctatcgct caataaggcg gtggcctacg aaacagctcg
    29641 taccgtggag tatctagctc atcagggatt ccccatctat ccccaaaccc ttgaaaccta
    29701 taacgccttt gtgcactatt tgaaagagga ctaaatgaag acggctttta taatcattga
    29761 tgttcagaat gttttagtag aaactgggtt ccagacaaaa agtctattgg aaaaaatttc
    29821 ttatttacaa aaccaggcta gaagcaagaa tattgaaatt atctatgttc aacatattga
    29881 gaactctgaa gctcaaacat cagaaggttg gcagttatct gcgcttttaa atcgaaaacc
    29941 tgctgaaaag gtctttcaga agaagtataa cagtattttc aaagaaactg gcttaaaaga
    30001 atacttggat aaacagggga ttgaaaaatt agttttatgt ggtatgcaga cagaatattg
    30061 tgtggatacc tctgtcaagg ttgcctttga atatggctat cagcttattg ttccagaagg
    30121 tgctgtcaca acctttgatg gggatgacat tccagcagaa acgattaatg aattttatga
    30181 ggacatttgg gaggagcgct ttgcagatgt cctagattac aaacatattt tctaaaaaga
    30241 ggattaaatg aacgaaaaag aattactaga actagtcgtg aaagcggctg atgagaaacg
    30301 tgcggaggat atcctcgcac ttgatgtaca agatttgact agtgtgacgg actactttgt
    30361 catcactagc tcaatgaata gccgtcagtt ggacgctatc gcagctaata tccgtgaaaa
    30421 agtagctcaa gcaggcttta aaggtagcca tgtcgaaggc gatgcagctg gaggctgggt
    30481 cttactggac ctcggtgctg ttgtcgtgca tatcttttca gaagaaatgc gtgcccatta
    30541 taatctagag aagctatggc atgaggcgaa ttcagtagat atttcagaag ctcttgctta
    30601 gaagatgaac tcagttgtag tcaactgggt ttctttgctt attttagaaa aattgataga
    30661 aaggtaaagg gctagtggtg tttaccatgg agctcttggt atcatgatta tggcgactta
    30721 tgaaaccttt gcggcggtct atgatgcggt catggacgat agtttatacg acaaatggac
    30781 gaatttttct ctgcgtcatt tgcctaagac caaggagaga aagaaactct tggaattggc
    30841 ttgtgggaca ggaattcaat cagtgcgctt ctctcaggct ggttttgatg tgactggact
    30901 tgacttgagt gcggatatgt tgaagattgc ggagaagaga gcaacttcag ccaagcaaaa
    30961 gattgctttt atagaaggta atatgctgaa tttgtccaag gcagggaaat acgattttgt
    31021 cacgtgttat tcggactcta tctgctatat gcaggatgag gtggaagtag gggacgtctt
    31081 taaggacgtg tacaatgtgc tgaatgaaga gggagttttc atctttgacg tgcactcgac
    31141 ctaccagaca gatgaagtat tccctggcta ttcctaccat gaaaatgcgg aagattttgc
    31201 catgctttgg gatacatatg aggacgaagt tcctcactcc atcgtgcatg agctgacttt
    31261 ctttatcaag gaggctgacg gttcctttag tcgccacgat gaagtacatg aggagaggac
    31321 ctatgagatc ttgacctatg atatcttgct ggaacaggct ggatttaagt ccttcaaact
    31381 ctatgcagac tttgaggata aggaaccaac agaaactagc acccgttggt tttttgtggc
    31441 gcagaagtag gagaacatca tgaccatcac aggtattatc gcagagttca atccttttca
    31501 taatggccat aaatacctgc tggatcaggc agagggactg aaaattgttg ccatgtcagg
    31561 gaatttcatg cagcgtggag agcctgctat tgtggataag tggacaaggg cccagatggc
    31621 gctggaaaat ggcgcagact tggtagtgga attgcccttt ttagtcagtg ttcaggcagc
    31681 tgatttcttt ggtcaaggag ctgtggatat cttggatcgc ttgggcattg atagtctagt
    31741 ttttgggaca gaggaagttc gagattatca gaaaatcgct gacttataca cagagaaagg
    31801 tactgagatg gagaaatttg tggaaaatct gcctgattcc ctttcctatc cccagaaaac
    31861 ccaagctatg tggaaggaat ttgctggtct tgatttttca ggtaataccc ccaatcatgt
    31921 tcttgctctg gcctatgcaa aggcggttgc aggacgaaat atcaagctgc atccgattca
    31981 gcgtcagggg gcaggttacc attctgtgaa taaagatgtg gactttgcct cggcgacagc
    32041 cctccgtcaa catcagaagg accaagattt cttagaacgc tttatgcctt ctgttgccct
    32101 atttgagcag gccagtaagg tgatctggga agactatttt cccttgctcc gctatcaaat
    32161 cttgtcaaat cccgacctaa ccaccatcta tcaggtcaat caagaaatgg ctgtgcgcat
    32221 taaagaagct attaaaacag cccagtctgt ggaagaattg gtcgagttag tgactaccaa
    32281 acgttacacc aaggcgcgtg tcagacgcct attgacctat attttgatgc aggctagaga
    32341 aagtgacttg ccagaagcca ttcatgtcct tggatttact gaaaaaggta ggcaacatct
    32401 caagtctttg aaaggtcagg tcagtctagt cagccgaatt ggcaaagaac cttgggatgc
    32461 tatgactcaa aaggctgacc agatttacca actaggaaag ccaagtatag cacaacagaa
    32521 ttttggaaga gtgccgatta gaatagaaac aaactaagtc taccgaaagg tggacttttt
    32581 gagatttttt acgaataata ctcttcgaaa atcaaattca aatcgcgtca acgtcgcctt
    32641 gccgtactca agtacagcct gcggctagtt tcctagtttg ctctttgatt ttcattgagt
    32701 ataaatagat agaaaagaaa atcttgtaca agttcctgac aatttctttc ttttcgtgta
    32761 taatagtgga aaagagctaa aacgaggtat ggttatggaa gcagtccttt actcaacatt
    32821 ccgaaatcat ttaaaggact acatgaagaa ggtaaatgat gaatttgagc ctttgacggt
    32881 ggtcaataaa aatccagatg aggacattgt agtcctttca aagagtgagt gggatagtat
    32941 ccaagaaacc ctgagaattg ctcaaaataa ggaactttct gataaggttt tgcgaggaat
    33001 ggctcaagtt cgtgctggaa gtactcaggt ccatgttatt gaggagtaag aaatgctgct
    33061 caagtttaca gaagatgcct gggcagatta ttgctactgg caaaaccaag ataaaaaaac
    33121 actaaaaaga atcaataaat taatcaagga tattcaacgc gatccattta caggaatagg
    33181 aaaaccagag ccactcaaat atgattacca aggagcctgg tcacggcgta ttgatgcaga
    33241 aaatcgcttg atttatatga tggatggaga tagcgtggct ttcttgtcct ttaaagatca
    33301 ttactaagtc tactgcaaag taggcttttt ctcaagtctc tactaaacta ctatttctaa
    33361 acaacaagtt tattttggta aatgtgttcc aaattcccca aaacatattc tattttaggt
    33421 ttgtgaaaat cactttttta tggtagaatg taaaagaatg tatgtcattc ggaataacaa
    33481 taaaatgagg taaaaacatg gaaatcatgt cgcttgcgat tgctgttttt gccgtcatca
    33541 ttggtttagt cattggatat gtcagcatct cagctaagat gaaatcatct caggaagctg
    33601 cagagttgat gcttttaaat gctgaacaag aagcaactaa tttacgtgga caagctgagc
    33661 gtgaagcgga tttacttgtt aatgaagcca aacgtgaaag caagtctctt aaaaaagaag
    33721 cactattgga ggccaaagaa gaagccagaa aataccgtga agaagtggac gctgaattca
    33781 aatcagaacg tcaagaactc aaacaaatcg aaagtcgttt gacagagaga gctactagcc
    33841 ttgaccgtaa ggacgacaat ttgacgagta aagaacaaac acttgaacaa aaagaacaaa
    33901 gtatttctga tagagcgaaa aaccttgatg cgcgtgaaga gcaattagag gaagtcgaaa
    33961 gacaaaaaga agcagaacta gagcgtattg gtgctctgtc tcaggcagaa gcacgagata
    34021 ttatcttggc tcagacagag gaaaacttga ccagggagat tgccagtcgc attcgcgaag
    34081 ctgagcaaga ggtcaaggaa cgttctgaca aaatggccaa ggacatcttg gttcaagcta
    34141 tgcaacgtat cgctggtgaa tatgtagcgg agtcaacaaa ctcaacggtt catctgccag
    34201 acgatactat gaagggacgc attattggtc gtgaaggtcg taacattcgt acctttgaaa
    34261 gtttgacagg ggtcgatgtg attatcgacg atacaccaga agtggtgacc ttgtcaggat
    34321 ttgatccgat tcgtcgtgag attgcccgta tgactatgga aatgttgctc aaagatggtc
    34381 gtatacatcc agctcgtatc gaagagttgg ttgagaaaaa ccgtcaagag attgacaata
    34441 agattcgtga atacggtgag gctgctgcct atgaaattgg tgcgccaaac cttcatccag
    34501 acttgatgaa gattatggga cgtttgcagt tccgtacttc atatggacaa aatgttttgc
    34561 gccattcgat tgaggttgct aagttggctg gtatcatggc gagcgaactt ggtgaaaatg
    34621 cggctcttgc ccgtcgtgct ggattccttc acgatatcgg gaaagccatt gaccatgagg
    34681 ttgaaggtag ccacgttgaa atcggtatgg aattggcccg taagtacaag gaacccccag
    34741 ttgtggtgaa tacgattgct agtcaccacg gagatgttga agctgagagc gtgatagcag
    34801 ttatcgtcgc tgcagcagat gccttgagcg cagcccgtcc aggtgctcgt agtgagtctc
    34861 ttgaaagcta catcaagcgt ctccatgatt tggaagaaat tgctaacggc tttgaaggag
    34921 tgcaaactag ctttgccctt caagcaggac gtgaaattcg tatcatggtc aatccaggaa
    34981 aaatcaagga cgacaaagtc acaatcttgg ctcacaaagt tcgtaagaaa attgaaaaca
    35041 atctcgatta tccaggaaat atcaaggtaa ccgtgattcg cgagcttcgt gcagtagatt
    35101 atgctaaata aataagaaag agcagttgaa aaattactgc ttttttgtta cactagatag
    35161 aaagactgta aaggataatc tggctttttg ccattattct agagaaatgt tatttcacag
    35221 attgactaaa aggagacaca atggcagacc gaggcttact aatcgttttt tctggtcctt
    35281 caggggttgg aaaaggaacg gttagaagag agatttttga gagttctgaa aaccaatttc
    35341 aatattctgt atcgatgacg acacgcgcac aacgtcctgg agaagtggac ggtgttgact
    35401 acttcttccg tactcgtgaa gaatttgaag agctgattcg tcaaggacag atgttggaat
    35461 acgcagaata tgtcggcaac tactatggaa ctcctctgac ctatgtcaat gaaaccttgg
    35521 acaagggaat cgatgttttc cttgaaattg aagttcaggg tgctcttcag gtcaagaaaa
    35581 aggttccaga tgctgtcttt atcttcctga caccaccaga tttggatgaa ttgcaagatc
    35641 gcttggtagg tcgtggaaca gatagtgcag aagtgattgc ccaacgaatc gaaaaggcca
    35701 aggaagaaat tgccctcatg cgtgagtatg attatgcgat tgtcaacgat caggtgcccc
    35761 tagctgctga acgtgtcaaa tgtgtgattg aagcagaaca cttctgtgtg gatcgtgtca
    35821 ttggtcacta tcaggagatg ttaccaaaat ctccaactac ccgataaaat ttagaaaata
    35881 ggtacaagca aatgatgtta aaaccctcta ttgatacctt gctcgacaag gttccttcaa
    35941 aatattcact cgtaatcttg gaagcaaaac gtgcccacga attggaagca ggtgccccag
    36001 caactcaagg tttcaagtct gaaaaatcaa ctcttcgcgc tttagaagaa atcgaatcag
    36061 gaaacgttac aattcaccca gatccagaag gaaaacgtga agcagtgcgt cgccgtatcg
    36121 aagaagaaaa acgccgcaaa gaagaagaag aaaagaaaat caaagagcaa attgctaaag
    36181 aaaaagaaga tggtgaaaaa atttaaggtt ggggggactc aatcttattt tttctattgc
    36241 aagaatgtac tgacaaggag gaggtgagaa gatggcccta gctaagatta tcgtagatgt
    36301 gcccttgatg cagacggacc agccctatag ttacaggatt ccggaggaat ttgagggaat
    36361 gctggaagtt gggatgcggg ttcatgtgcc ttttggtaag ggtaatcgcc tgattcaagg
    36421 gattgttttt ggtttggagt cccaatcaga tggagaagag atggagcaag atttaaaaga
    36481 tattgcagag gtgctggatt tttctcctgt tctcacgcca gaacaactct ggctggctga
    36541 ggagttacgc aaatctgtct tctcctacaa aatctctatc ctcaaggcca tgcttccggg
    36601 atttctgaat tctagctatg acaagattct ctatcctctg gaaggcttga gtcaggaaga
    36661 acgagtgcgc ctgtttggtt cagaagattc gctagccttt tcttctctgg atttaggtaa
    36721 gcaagctgaa atgatgcgtt tgactagaaa aggcctgctt ggtctggaat atcaggcagt
    36781 cgatcaaaag aaggtcaaga cccagtcttg gtatgaggtt gaccatgctc aattagaagg
    36841 tgttgagatt tctacacgtg ccaagaaaaa gttggaactg agagattatc tgctgtctca
    36901 tccagagagc gcttccttgg ctagcttgtt agagtcctac tcgcgagagc aagtcaactt
    36961 ctttgtggat caaggtgctg ttaccatagt ccaaaaggaa gttcgacgct cggctgctta
    37021 ttttgaagga attgaagcaa gtagaccttt ggagttaaat ccagaacaaa gacaggcgcg
    37081 tgatgcggtt gtgagttcta ttggcagttc tcaacctccc tttctacttc aagggattac
    37141 aggaagtggg aagaccgagg tttacttgca gattatccaa ggtgccctgg ataagggcaa
    37201 gacagctatt ttgctggtac ctgagatttc cttgactcct cagatgaccg agcgttttat
    37261 agcacgtttt ggggacaagg tagccattct tcactcagga ttatccaatg gcgaaaagta
    37321 tgatgaatgg cgcaaggtgg agcgtggcga tgcccaagtt gttgttggtg ccagatctgc
    37381 tatctttgct cctctgaaaa atctgggtgt catgattatt gatgaagagc atgaagcggc
    37441 ttataagcag gacagcaatc cccgttacca tgccagagag gtagctattt tacgggctca
    37501 gtataatcaa gcaactctgg tacttggatc tgcaacgccg agcttagaga gccgggcacg
    37561 tgctggcaaa ggcgtctatc aacacttacg tctaacccaa cgtgccaatc ctttggctac
    37621 aatccctgag gttcaagtga ttgactttcg agactatatc ggacaaaatg agacgtcaaa
    37681 ctttacgcct cctttgctag aggctatcca agaccgtctg gttaaaaaag agcaggtggt
    37741 tctcatgctc aatcgccgtg gttattctag ctttgtcatg tgtcgggagt gtgggacggt
    37801 ggatacttgt cccaactgcg atatttctct gaccttgcat atggatacca agactatgaa
    37861 ctgccattat tgtggtttct cgaaggatat tcctcaggtc tgtcctaact gtaagagcca
    37921 cagtattcgt tactatggga cgggaactca gaaggcttat gatgagctag cagaactctt
    37981 tccccaagct cgcattttga ggatggatgt ggatacgact cgaaagaaag gcagtcacca
    38041 agccttgctt gaccagtttg ggcgagggga agcggatatt ttacttggta ctcagatgat
    38101 tgcaaagggt ttggattttc ccaatgttac cctagtcgga gttctaaatg cggatacggc
    38161 cttgaatctg cctgatttcc gttcttctga gagaaccttc cagctcttga ctcaggtggc
    38221 aggtcgagca ggtcgggctg aaaaagcagg tcaagtcttg atacagtcct acaattcgca
    38281 gcactatgct attcgatttg ccaaggatca agattacgaa ggcttttatg cctatgaaat
    38341 gggaatcaga cgacaactcg gctatccacc ttactatttc acgattggta ttaccctttc
    38401 tcacaagaaa gaagaagagg ttttcaaacg tgcctatgaa gtcatgaaca ttttgcggtc
    38461 aggcttgtca gagaccagtc ccattctggg gccaacgcca aaaccgattg cccgtaccca
    38521 caacctctat cattaccaga ttttaattaa ataccgttta gaggatgagc tggggccgac
    38581 cctcaaccag gttttagcct tgactcaaga acgggaaaat agtgagctcc gtctcagcat
    38641 tgaccatgag ccgcaacaat ttttataaga aggagaagat atgacaaaac taatctttat
    38701 ggggaccccc gacttttcag caacagtctt aaaaggactt ttgacagata accgttacga
    38761 aattctagcc gttgcgaccc agccagaccg tgctgttggt cgtaaaaaag ttatccaaga
    38821 aaccccagtc aagcaggctg ccaaggaagc aggactatct atctaccaac ctgaaaaatt
    38881 atctggaagt ccagagatgg aagatcttat gaagctagga gcagatggaa ttgtgactgc
    38941 tgcttttggg cagtttctcc caagcaaact ccttgatagc atggactttg ctgtcaacgt
    39001 tcatgcctcc ctccttccta gacaccgtgg tggtgcgcct atccattatg ccttgattca
    39061 aggggatgag gaagctggtg tgaccatcat ggaaatggtt aaggaaatgg atgcaggaga
    39121 tatgatttct cgtcgcagca ttccgatcac agatgaggac aatgttggca ccttgtttga
    39181 aaaattggcg ctagttggtc gtgatttgct tttggacact ctgcctgcct atattgctgg
    39241 tgatatcaaa cctgaaccgc aggatacgag tcaggttacc ttctctccaa atataaagcc
    39301 agaggaagaa aaactggact ggaacaaaac caatcgtcaa ctctttaacc aaattcgtgg
    39361 aatgaacccc tggcctgttg cccatacttt ccttaagggc gaccgcttta agatttatga
    39421 agccctacca gtagaaggtc agggaaatcc aggtgagatt ctctctatct gcaagaaaga
    39481 attgattgtc gcaacggctg aaggggctct atccctcaaa caagtgcagc cagctggtaa
    39541 gcctaagatg gacattgctt ccttcctcaa cggagttgga cgtacattga ctgtaggaga
    39601 acgatttggt gactaaagta gaaacggcta gaagtttagc tctagcagtg ctagaggatg
    39661 tttttgtgaa tcaagcatat tcaaatattg ccttaaataa acacctcaag gggagtcagc
    39721 tctctgcagc agacaagggc ttagtgaccg agctagtcta tggaacggta gcccgtaaac
    39781 tgactctgga atggtaccta tcccacttta tcgaagacag agaccagtta gacagctggc
    39841 tctatgtcct tcttctcatg agtgcctacc aactccgcta tttggacaag attccagatc
    39901 atgctgtggt caatgaagca gtggaattag ccaaactccg taaaaaaggc agtgaaaaat
    39961 tggtcaacgc tgtccttcgc cgtatcttgc gtgaaggctg gccagatatt gctagcatca
    40021 agcgaaaaaa caagcgtgac tccattgcct attctctccc agtttggcta gttgccaaac
    40081 tcaaggaaga atacggagag gagagagcaa aagccatctt tgaaagcctg ttagtgcgaa
    40141 acaaagccag tattcgtgta acagacctaa gtcgaaaaga ggaaatccaa gccttgttgg
    40201 aggcgaatga ttccctttta gcaaccactg gtctggttaa ggagcaaggg cattttgcag
    40261 ggcataattt gtttgcggat ggagccatta ccatccaaga cgagtccagt cagctggttg
    40321 ctccgacgct tgatttacaa ggtgatgagc gagtgcttga tgcctgtgcg gctccaggtg
    40381 ggaaaacagc ccatatagcc tcttatctta cgacaggtca ggttactgct ctggacttgt
    40441 acgaccacaa gttggattta attcaagaaa atgcccaacg tctgggaatt gaagatcggg
    40501 ttcaaactca aaaattagat gccagaaagg tgcatgagtt ttttgaccag gattcctttg
    40561 ataagatttt ggtggatgct ccttgttcag gaatcggtct tttgcgccga aaaccagata
    40621 tcaaatacaa taaagaaacg gcagatttcg cgtccttgca ggaaattcag ttagaaatat
    40681 taggtagtgt ttgtcaaata ctaggcaaag gtggtataat aacttatagc acctgcacta
    40741 tcgtctcaga ggagaatttt caagtcgtta aggccttttt agagagtcat cccgagttcg
    40801 agcaggtaaa actagaacat gaatgtaagg atatcatgaa agacggctgt atcctcatta
    40861 cacctgaatt gtatggaagt gatggattct tcatcagtca atttcgcaag atatcggatt
    40921 aggaaggaac tgacacatgg aaatttcatt attaacagat gttggtcaga aacgaacaaa
    40981 taaccaagac tatgtcaacc actatgtcaa tagagctgga cgtaccatga ttattttagc
    41041 tgatgggatg ggaggtcatc gcgcagggaa tatcgctagt gaaatggcgg tcacagacct
    41101 gggtgtagct tgggttgata cccagatcga tacagtcaat gaagtgcgtg aatggttcgc
    41161 ccattaccta gaaattgaaa atcaaaagat tcaccagctt ggtcaggatg aagcttacag
    41221 aggcatggga actactttgg aagtccttgc tattattgat aatcaggcta tctatgctca
    41281 tattggtgat tcgcgtatcg gcttgattcg tggagaagaa taccatcagt tgacgagcga
    41341 tcattccttg gttaatgaat tgctcaaggc tggtcaattg acaccagaag aggcagaagc
    41401 tcatccgcaa aaaaatatta tcacccagtc tattgggcaa aaagatgaaa ttcagcctga
    41461 ttttgggaca gttatccttg agtcaggtga ctatctcttg ctcaatagtg acggcttgac
    41521 caacatgatt tcaggcagtg agattcgtga tattgtaacc agtgatattc ctttagcaga
    41581 taaaacggag acacttgttc gttttgctaa caatgcagga ggtttagaca acattacggt
    41641 tgcccttgtt tctatgaacg aggaggatgc agaatgatcc aaatcggcaa gatttttgcc
    41701 ggacgctatc ggattgtcaa acagattggt cgaggaggta tggcggatgt ctacctagcc
    41761 aaagacttaa tcttagatgg ggaagaagtg gcagtgaagg ttctgaggac caactaccag
    41821 acggacccga tagctgtagc tcgttttcag cgtgaagcga gagctatggc agatctagac
    41881 catcctcata tcgttcggat aacagatatt ggtgaggaag acggtcaaca gtatcttgca
    41941 atggagtatg ttgctggact agacctcaaa cgctatatca aggaacatta tcctctttct
    42001 aatgaagaag cagtccgtat catgggacaa attctcttgg ctatgcgctt ggcccatact
    42061 cgaggaattg ttcacaggga cttgaaacct caaaatatcc ttttgacacc agatgggact
    42121 gccaaggtca cagactttgg gattgctgta gcctttgcag agacaagtct gacccagact
    42181 aactcgatgt tgggctcagt tcattacttg tcaccagagc aggcgcgtgg ttcgaaggcg
    42241 actgtgcaga gtgatatcta tgccatgggg attattttct atgagatgtt gacaggccat
    42301 atcccttatg acggggatag cgcggtgacc attgccctcc agcatttcca gaaacccctg
    42361 ccgtccgtta ttgcagaaaa tccatctgta cctcaggctt tagaaaatgt tattatcaag
    42421 gcaactgcta aaaagttgac caatcgctac cgctcggttt cagagatgta tgtggacttg
    42481 tctagtagct tgtcctacaa tcgtagaaat gaaagtaagt taatctttga tgaaacgagc
    42541 aaggcagata ccaagacctt gccgaaggtt tctcagagta ccttgacatc tattcctaag
    42601 gttcaagcgc agacagaaca caaatcaatc aaaaacccaa gccaggctgt gacagaggaa
    42661 acttaccaac cacaagcacc gaaaaaacat agatttaaga tgcgttacct gattttgttg
    42721 gccagccttg tattggtggc agcttctctt atttggatac tatccagaac tcctgcaacc
    42781 attgccattc cagatgtggc aggtcagaca gttgcagagg ccaaggcaac gctcaaaaaa
    42841 gccaattttg agattggtga ggagaagaca gaggctagtg aaaaggtgga agaagggcgg
    42901 attatccgta cagatcctgg cgctggaact ggtcgaaaag aaggaacgaa aatcaatttg
    42961 gttgtctcat caggcaagca atctttccaa attagtaatt atgtcggtcg gaaatcctct
    43021 gatgtcattg cggaattaaa agagaaaaaa gttccagata atttgattaa aattgaggaa
    43081 gaagagtcga atgagagtga ggctggaacg gtcctgaagc aaagtctacc agaaggtacg
    43141 acctatgact tgagcaaggc aactcaaatt gttttgacag tagctaaaaa agctacgacg
    43201 attcaattag ggaactatat tggacggaac tctacagaag taatctcaga actcaagcag
    43261 aagaaggttc ctgagaattt gattaagata gaggaagaag agtccagcga aagcgaacca
    43321 ggaacgatta tgaaacaaag tccaggtgcc ggaacgactt atgatgtgag taaacctact
    43381 caaattgtct tgacagtagc taaaaaagtt acaagtgttg ccatgccgag ttacattggt
    43441 tccagcttgg agtttactaa gaacaatttg attcaaattg ttgggattaa ggaagctaat
    43501 atagaagttg tagaagtgac gacagcgcct gcaggtagtg cagaaggcat ggttgttgaa
    43561 caaagtccta gagcaggtga aaaggtagac ctcaataaga ctagagtcaa gatttcaatc
    43621 tacaaaccta aaacaacttc agctactcct taaaagcaga tggataatca aaatgagtaa
    43681 tcaatctttg tcatgatttc atggcaaaga ttttttttga gtccagattt gtgatagaat
    43741 agagggagtt gataaaagga ggaaagcatg gaagaatcaa aagaattaaa tgccgtcatt
    43801 gatgtgatta tgctagcggg gactattctc cttaaaagtg gctcagaaat ccatcgtgta
    43861 gaagatacca tgattcgaat cgcgcattcg caggggattg tggattgcaa tgtccttgcc
    43921 atgcctgccg ctatcttttt ctctattgaa aataccaata tttcgcgcat gaagcgcgtg
    43981 acctcctctt cttataacat cgaaaaagtc tgcgatgtga accagatttc tcgtcagcta
    44041 gttggggggc agattgactt agaaacagcc tttaagcaat tgacggcctt gcaagctcaa
    44101 ccccttccct atactaagtt gcaggtaact ctggctgcga cctttagtgc tcctttcttt
    44161 tcagttatgt ttagcggaaa tatctacgac gcacttgggg caggagtggc gaccttattt
    44221 ggttttgcct tttccctcta tgtggaaaaa tttatccgaa ttccctttgt gacagccttt
    44281 gctggagcct ttgtctttgg gataattgcc cagttttggg ctcgctacac aggttttcct
    44341 tcaacggcag atttgattat agctggtgcg gtcatgccgt ttgtaccagg tattgccttg
    44401 accaatgcgg tccgtgatat tatgaccaac cacataaact ctggtatgag taagatgttt
    44461 gaatccctgc tcattaccct cgctttaggg gcaggaactt ctgtcgcctt ggtattgatg
    44521 aactaatatg acactaacaa cctttttatt acaagcagta gcaagttttc ttgccattat
    44581 cactttttta atcgtactca atgtgcaacg gtctatgctc ttacctggag ggattttggg
    44641 catgactgtc tggctaatct atctcttgct caaggaaccg accaatgtca ttgtagctac
    44701 cttcattgca gccattattg gttcttgtgt cagccagatt ttaagtattc tttataagac
    44761 acctgctgtg gtctttatct tggccatttt ggcaccgctg gttccaggtt atctctccta
    44821 ccgaacaact gccttttttg tgacagggga ctataataaa gcactggcaa gtgcgacctt
    44881 ggttgtcatg ttggctttgg taatctctat tggaatggct agcggaacag tgattctcag
    44941 actgtatcat tatataaaaa cacatcgagt atcgtagact ttacagaaat aaaagaattt
    45001 tctgaaaaat gagataaata aattgacaac gctttctata tgtgcgagaa taccgcactt
    45061 atgaagaaat tgcggctgat tttggtatcc acgaaagcaa cttaatccgt cggagccaat
    45121 gggttgaagt aactcttgtt caaagtggtg ttacgatttc aaaaactcat cttagtgctg
    45181 agaatacggt gattgtggat gcaacagagg taaaaataaa tcgtcctaaa aaaatcaact
    45241 agccaattat tctggtaaaa tagaaatgtc attctatgga acctcaagcg attgtcacaa
    45301 gtcaagggag aattgtttct ttggatatcg ctgtgaacta ttgtcatgat atgaagttgt
    45361 tcaaaatgag tcgcagaaat atcggacaag ctggaaaaat cttggctgac agtggttatc
    45421 aaggacccat gaagatatat cctcaagcac aaactccacg taaatccagc aaactcaagc
    45481 cgctaatagc tgaagataaa gcttataacc atgcgctatc caaggataga agcaaggttg
    45541 agaacatctt tgccaaagta aaaacgttta aaatgttttc aacaacctat cgaaatcatc
    45601 gtaaacgctt cggattacga atgaatttga ttgctggcat tatcaattat gaactaggat
    45661 tctagttttg caggaagtct actattttcc ttattgtctg taagtctact gaccttgttg
    45721 tttatcccag tcatggtttc tagttcgggc tcagagtttc aaagtggatg gcaagagcat
    45781 caattgattg ctgagaaggt tagtaaaaca cttgacaaga catttgataa ggatgtcaga
    45841 gaaattccga ccagtcagtt ttatcaaaaa tttgtagatg agatgggaag gacttactca
    45901 ggaaatttga tcctccagga gctgataact gtgaatggag cttataaagc tacttatatc
    45961 ggtgaactct ctagcaacta atcattcttt aaaatgatga ccaataagag gcttgatttg
    46021 gtgaatcaag tcttttttct ctcttttact gccatttgtg ataaaatagt atagaacgat
    46081 tttttacatg aatgataaaa cagaggtaaa tatgacaatc ggtattgata agattggttt
    46141 tgcgaccagt caatatgtct tgaaattaca agacttagca gaagcgaggg gaattgaccc
    46201 tgaaaaatta agtaaaggac tcttactcaa ggaattgagt attgcgcccc taactgagga
    46261 tatcgtgacc ttggcggcca gtgctagtga ctctatttta actgagcaag aaagacaaga
    46321 agttgacatg gtcattgtgg cgaccgagtc aggaattgac cagagtaagg ctgcggccgt
    46381 ctttgtgcat ggcttgctgg gcatccagcc ctttgctcgt agtttcgaga ttaaagaagc
    46441 ctgttatggg gcgacagctg ccctccatta tgccaaattg catgtggaaa attctccgga
    46501 gtccaaggta ttggtcattg ccagtgatat tgccaaatat ggtattgaaa ctccgggaga
    46561 accaactcaa ggtgctggaa gtgtagctat gttgattaca caaaatccac gcatgatggc
    46621 ctttaataat gacaatgtag ctcagacccg tgacatcatg gatttctggc gaccaaatta
    46681 ctcgacaact ccttatgtaa atggtgttta ttctacccaa caatacttgg atagtttgaa
    46741 aacgacttgg cttgaatatc aaaaacgcta ccagcttact ttggatgatt ttgctgctgt
    46801 ttgtttccac ttgccttatc ctaaattagc gctaaaaggc ttgaaaaaaa tcatggataa
    46861 gagcctgcct caagagaaaa aagacctctt acaaaagcat tttgaccagt ctattctcta
    46921 cagtcaaaag gtggggaaca tctacacagg ttcacttttc cttggacttt tgtctctctt
    46981 ggaaaataca gatagcttga aagctgggga taaaatcgcc ctttatagtt acggaagtgg
    47041 agctgtggct gagttcttca gtggtgaatt ggttgaagga tatgaagctt atttggataa
    47101 agaccgtttg aacaagctca accaacgaac tgccctatcc gttgcagact atgaaaaggt
    47161 cttttttgag gaagtaaact tggatgaaac aaactctgcc cagtttgctg gctatgaaaa
    47221 tcaagatttt gccttggttg aaattcttga ccaccaacgc cgttatagca aggttgaaaa
    47281 ataatgaaga taagttggaa tggattttct aaaaaatcat accaagagcg cctcgagctg
    47341 ttaaaagctc aggcgctcct tagtcctgag agacaagcta gtctggagaa ggatgaacag
    47401 atgagtgtga ctgtggcaga ccagctgagt gagaatgtgg tgggaacttt ttctctgcct
    47461 tattcgctgg ttccggaggt acttgtcaac gatcaggaat acaccgttcc ctatgtgaca
    47521 gaagaaccct ctgtggttgc ggcggccagc tatgccatca aaatcatcaa gcgtacaggt
    47581 ggttttactg cacaagtcca tcagcgacag atgattgggc aggtagccct ttatcaagtt
    47641 gctaatccta aactagcgca agagaagatt gccagcaaga aagcggagct cttggagctt
    47701 gccaatcaag cctatccttc tatcgttaaa cgtggaggtg gggcgcgtga tctgcatgtc
    47761 gagcagataa aaggcgaacc agactttctc gttgtttata ttcatgtcga tacccaggaa
    47821 gccatgggtg ccaatatgct caacaccatg ctggaagcct tgaaaccagt cttagaagaa
    47881 ctcagtcagg gacagagtct catgggaatc ctgtccaact acgcgactga ttctctggtg
    47941 actgcaagct gtcgcatcgc ctttcgctac ttgagccgcc aaaaggatca aggacgagag
    48001 attgcggaga aaattgcgtt ggctagtcag tttgcgcagg ctgatcctta ccgagctgct
    48061 actcataata aaggaatttt taatggtatt gatgcgattt tgattgccac tggtaatgac
    48121 tggcgtgcca tcgaagctgg ggcccatgcc tttgccagtc gagatggacg ctatcaaggt
    48181 cttagctgct ggacgctgga ccttgaaaga gaagaattgg tcggtgagat gaccctgccc
    48241 atgcctgtag cgactaaggg tggctctatc ggcctcaacc cacgtgtagc tctcagtcat
    48301 gatctactag gaaatccttc tgccagagaa ttagcccaga ttatcgtgtc catcggtctt
    48361 gctcaaaatt ttgcagccct caaagccttg gtaagtacgg gcatccagca aggccacatg
    48421 aaactacagg ccaaatctct agctctccta gctggggcta gtgaatctga agttgctccc
    48481 ctagtagagc gcctcatctc agataaaacc tttaacctag agacagccca gcgctatctc
    48541 gaaaatttaa gatcataaaa actcagacga atcggtctga gttttcttgt gtttaaatac
    48601 tttttcctgg taatagcgta actggtaaga agtaaccagt tgtggcgact tccttgcctt
    48661 caatcttttg caagagaaga tcaatagtga gacaagcaat ctcttccaaa ggttgcttga
    48721 tagtagccaa ttgagggtag taattttcga taaagtaggt cccatcatag ccgatgacct
    48781 tgagctcttt tgggacagaa atgcccaatt cttgagcgat tttaatgacc agaatagctg
    48841 tcaaatcatc cgaagcaaaa atggcatctg gtttttcccg ggtcaagata ttcttgattt
    48901 ccatttcttt tctgacggga gaaaagtcac tggaaacatt gataatagga gcttttggga
    48961 gtacggatgc aaaaccagcg tggcgcagtc cggttggcga attagaattg tcattccctg
    49021 taatcatgat gatagactgg gcacctgtct tgaccaaggt ttgggcagca agaaccccac
    49081 cagcatagtt gtcagaggag acgacaggga tgtctggcga taggtttcgg tcaaaggaaa
    49141 taatcggcgc tgtcacacga ttgtagtctt cgattcctag gttgtgacta ccagaaatga
    49201 tgccgtccac ctgattggct tccaacattt cgatgtattc gcgttccttc tcagaatcat
    49261 gttcactgtt gcagatgatg gtcttgtaac catttttgaa gagttggtgt tccaatttat
    49321 caatcaattc tgcatagaaa acattggaaa tattggggaa aatcaagccg attaacttag
    49381 ctgattttcc ttgcagacta cgagccaggt tgttgggttt atagcccaat tctctcatgg
    49441 cttcattgac tttttggatg gttttctcag atagataccc ttttttattg ataacccgag
    49501 aaacggtagt aggactgacg cctgcaagtt tggcgacatc agttagtttt gcgaccataa
    49561 tctaattcat agtaagttcc agttgggttt ccagatttga tcaggatacc attttggtcc
    49621 gcatgtggga agacacgacc agaaaatact ttttctcctt tattgatgaa aatttcaaag
    49681 acagagttat cgatgaagat tgtagcagta gtagcctgat tctcgatagg gcaagaacga
    49741 gttgtcccaa attcttgggc atactgctct ccagcctggc tacgatccac tgtcacttga
    49801 ccgtttacaa ggtcaaagtt gattgaaagt cccttacctt ctttatcagc aagtaagaca
    49861 atctcgctct ggctattagc ttccaagttg agttcaagtt cgtaagtgtt cttggtttgg
    49921 gaacggtttg agaaggcttc ttcagaagca cgaaggtcct taatagcagc gactggatac
    49981 tggtagagct tgtcgtcttt gatagtgagt tccttgacca aagagaaggt tccttggtgg
    50041 tcaaaacggt cagatgggta agaaacatct ggcaaaccaa gccagctaac tgctagagca
    50101 cgcccatcag gagcgttgaa ggcttgagtt gcataggctt cgaaaccgta atccatgttt
    50161 tgaagttgag acacatctac cattttggca tttttagggt caaaggaagc cccaatctta
    50221 tacatatttg gaaagatatt atcgtagtct agaactttct tatccaatcc ttgtggacag
    50281 tagagaagga caggatgttc ctctacaaag accagattag gacattccat catgtaggca
    50341 gtacggtcgt tagcaaagtc aaggtcgcca actgcttgcc agtttgtgta gtcgttattg
    50401 acagccttgt agagacggac gaagcctttt ttctccaagt cctgtccacc gacgacagca
    50461 tagtattgac ctttaaagtt aaaaatttgt ggatcgcgga agtggtcagt agagtctgct
    50521 ggctggtcaa tcaagatctt gtcaatcttt gtaatcttac cctccttgtc catcaaagca
    50581 ccgatctggt atgggtgacg gatccagttt tcatcgcgaa catttcctgt ataaaatagg
    50641 aataagttat cgccaaattg catggcagaa ccagagtagg caccgtggct atctaatgga
    50701 gtatctggta aaactttgat tccagtttct ttaaagtgaa ccaaatcatc actttctagc
    50761 tgtgcccaag atttcaaacc gtgggctgca ccaaaaggaa aattctggta aaagaggatc
    50821 cacttgccat caaagtaaga aaagccattt gggtcgttga gaagtcctgt ttttggctca
    50881 acatggtaat gagtatgcca tggagattgt gccatatttt cctttatatg ttgaatttct
    50941 tcttgcgtcc aatcttgata aagtctgtaa cgacgctcag ttgtccattc cattcattcc
    51001 tccaaaaatc ttatcacttt taatagtaac tgttttcgat aaaaaatgca agccttttat
    51061 attaaaaaag agaaaaaact gtcaaacgat taccataaaa taaatacagg aaatcaatca
    51121 aatttttacg aaaatgtgaa agataatgaa ataaaagact ttttaaccaa ggtttcaaac
    51181 ttatttttca agaaattcct gatgaaacag tcaaaaacaa tcaatctaaa gcttgcccac
    51241 taagtagata gaatattttt tctgcaaaac gcttgacata tttttaaaac atgataaaat
    51301 aatagtcgta agggcgaata aaatcgcttt caaacaagaa caaaatgtta tataaggaga
    51361 tttttgcaaa tgaacaatca ggaaattgca aaaaaagtca tcgatgcctt gggcggacgt
    51421 gaaaatgtca atagtgttgc ccactgtgcg actcgtctac gtgtcatggt caaagatgaa
    51481 gagaaaatca ataaagaagt gattgagaac ttggaaaaag ttcaaggtgc tttctttaac
    51541 tcagggcaat accaaattat ctttggtaca ggtacagtta acaaaatgta cgatgaagtt
    51601 gttgtacttg gcttaccaac atcatctaag gatgacatga aagcagaagt tgctaaacaa
    51661 gggaactggt tccaacgtgc tatccgtact ttcggtgatg ttttcgttcc aatcatccca
    51721 gttatcgtag cgacaggtct cttcatgggt gtgcgtggtc ttttcaacgc tcttgaaatg
    51781 ccacttccag gtgactttgc aacttacaca caaatcttga cagatacagc cttcatcatc
    51841 ttgccaggtt tggttgtgtg gtcaaccttc cgtgtatttg gtggaaatcc tgccgttggt
    51901 atcgttcttg gtatgatgct tgtctctggc tcacttccaa acgcttgggc agttgctcaa
    51961 ggtggtgaag taacagcgat gaacttcttt ggtttcatcc ctgttgttgg tttgcaaggt
    52021 tccgttcttc cagccttcat catcggggtt gtcggagcta aatttgaaaa agctgtccgc
    52081 aaggttgttc cagatgtcat tgacctcttg gtaacaccat tcgtgacact tttggtcatg
    52141 tctatccttg gactctttgt cattggacca gttttccacg ttgttgaaaa ctacatcctt
    52201 attgctacaa aagcgattct tagcattcca tttggtcttg gtggtttctt gattggtggg
    52261 gttcaccaat tgatcgtcgt gtcaggtgtg caccacatct tcaacttgct tgaagtgcaa
    52321 ttacttgctg ctgaccatgc taacccattc aacgctatca tcacagctgc tatgacagct
    52381 caaggtgctg ctactgttgc ggttggtgtt aaaacaaaaa atccaaaact gaaaacactt
    52441 gctttcccgg ctgctctttc tgccttccta ggtattacag agcctgctat cttcggggtg
    52501 aacttgcgct tccgtaaacc attcttcctt tcattgattg ctggtgcaat cggtggtgga
    52561 ttggcttcta tccttggact tgctggtact ggtaatggta tcaccatcat ccctggtaca
    52621 atgctttatg ttggtaacgg acaacttcca caataccttc ttatggtagc tgtatcattt
    52681 gcccttggtt ttgctcttac ttacatgttt ggttacgaag atgaagtaga cgcaactgca
    52741 gctgcaaaac aagctgaagt ggctgaagaa aaagaagaag ttgcgccagc agctcttcaa
    52801 aatgaaacac ttgtaactcc tatcgttggt gatgttgtcg ctcttgctga tgtcaatgac
    52861 ccagtcttct caagtggagc tatgggacaa ggtatcgctg tgaaaccaag ccaaggcgtg
    52921 gtctatgcac cagctgatgc tgaagtttca attgcctttc caacagggca cgcttttggt
    52981 ttgaaaacaa gaaatggtgc tgaagttttg attcacgttg gtattgacac tgtatctatg
    53041 aacggtgacg gttttgaagc aaaagttgct caaggtaata aggtgaaagc tggcgatgtt
    53101 cttggaacat ttgactcaaa caaaatcgct gcagctggac ttgatgatac aacaatggtt
    53161 atcgttacaa atacagctga ctacgcttca gtagctccag tcgcaacagg ttcagttgcg
    53221 aagggggatg ctgtgatcga agtgaaaatc taatcaatcc tctctaataa aaacgaacaa
    53281 atgacatgtt tgttcgtttt tattagatag taagatttac agaggaaaat ctaaaaaata
    53341 gagacattta gactttcgaa gtatgctata ataaagaaaa taaaaacaag aggtttatca
    53401 tgacaaaatt atatggaagc ttggaagcgg gcggtacaaa gtttgtctgt gctgtcggtg
    53461 atgaaaactt taacgttgta gaaaaaacac aatttccaac aacaactcca atcgaaacaa
    53521 tcgataaaac cattgagttc ttctcaaaat tcgataacct tgctggtctt gcagttggtt
    53581 catttgggcc gattgatatt gacaaaaact caaaaactta tggctttatc acgacgactc
    53641 caaaaccaaa ctgggcaaat gtggacttgc ttggtgccct tcgtcgcgcc ctaaacgtgc
    53701 caatgtactt cacaacagac gtaaacagct ctgcttacgg tgaagtggtt gcccgtaaca
    53761 atgctggtgg tcgtatcgaa aacttggttt actacacaat cggtacaggt atcggtgcgg
    53821 gcgtcatcca acgtggtgag tttatcggtg gtgtgggtca ccctgaaatg ggtcattatt
    53881 atgttgctag acacccaatg gatattgaaa aagagtttaa gggtgtttgt cctttccata
    53941 agggatgtct agaaggttat gcagctggtc caagtttgga agctcgtaca ggtgtacgtg
    54001 gggaaactat tgaactcaac aaccctgttt gggatgttca agcctactat atcgctcaag
    54061 ctgcggttaa tgcgacagtg actttccgcc cagacgtgat tgtctttggt ggaggggtca
    54121 tggctcaaca acatatgctg gaccgtgtcc gtgagaaatt tacatctctt cttaatggtt
    54181 acctaccagt accagatgtg cgtgactata tcgtgacgcc agcagtcgca ggaaatggtt
    54241 ctgccacact tgggaacttt gttcttgcaa aagaagtttc aaaataaagc acaaaatccg
    54301 cttgggaaac caaacggatt ttgtggttga aaacaatatt tttgagcctt tccttaagtg
    54361 attacggacg gtagcgactt tcttcgaaat tccataccta aactttgaga cactaggctc
    54421 aaagtttccg aatacctgaa accattagat ttcaggtgtt tctatcacgg cggaaaggct
    54481 aggtaagtcc tcgcctcact atcgaaaaat atgcatgatt tccataccaa agtatattta
    54541 gtatcaagct atagttaatc tacgctttaa aaatccactt gggaaaccaa acggattttt
    54601 tacgtgtcaa agcatttgcc agaaaaagtc aataatatag gtcaaaccgt aggtataaac
    54661 cacgagggta aagggtttgg tcagaatgat gatggtcatg tagcgcttga aactcatctt
    54721 ggtcagggca gccagcatac agagaaagtc agctgggcta atgggccaaa tcatcataaa
    54781 aataaagaag cggtcaaaac gattgccctt atctagccag ccgatgtact tgtcgtaggt
    54841 gcgcttgctg acgacagact ggacaaaggc agctccgtat aggcgcacta gataaaagat
    54901 aatggcacag ccaatcacga tgccgatata gttgtagata gtcccgatga tgtgcccgta
    54961 gataaagacc ccagccaccg aggtcaaggc ccctggaatg atagggacga cagtctgtaa
    55021 aatctgtaaa aagataaaga gaggtggacc ccagatgcct gcctgctgga taaaggcaga
    55081 gagggtttcc ttggattgta aaatcccagc ctgataagcc caaatacaga aaatcaaact
    55141 cccaacccca ccgacaatcg atgagatatt gataactcgc tgcagaaggg gagaaacagc
    55201 tttcatttgt ttatcctgtg acatgtaaac tcctcataga tagtatattt ctactatacc
    55261 atattttgga ggagaaaggg ggaatgagtc ttttttgata aggaatagaa agtaaatagc
    55321 ccttcgagac ttagaatagg tctgtcaact aaaggttata tgcattctag gtttcgaata
    55381 atcgcaattc catgaattta aaagcattct aagtttcgaa tagcttatct gagataattt
    55441 tgaggcattc gcgctttcga atagtcattt tccctaaagt ttagatgtat tctgaagcta
    55501 gaaagactaa aatctaaaag tagtaagagt ctctctctga tggacgtaat ttatggctag
    55561 tatgaatcct ttgaattgtt agttaatttt aatattaatc agttctgaaa taattactaa
    55621 atatagttag atggtgaatt aattattgct aaatttcaag ttcaagttag tcatccagaa
    55681 gtcttctctg ggggacttgt agttcaagca ttttttagga tagttgttaa tccacttttc
    55741 gatgaatgcg acttcctttg tagtcgtttt cttagttccc tgaggtaacc atctatgaat
    55801 gaacttgttg tgatttttat tagatcctat agtggtgtgc ataatagata taatagattg
    55861 aaactagaat agtacgcatc tacttctaaa acattgttag aaatcgattt gactgtcccg
    55921 atctatttgt cctgttttta tttcattttg ctatataatc cttagaaaac atatgggaca
    55981 agcggttgaa ttccgttccg tccattagta gataaaggat tactccttct tgaaatgaca
    56041 gatgaacttg tttcttataa aaatgttgtc tgaaactatt cgcttttttc cagaagtttt
    56101 agaggaagaa aactttatga ggaaaaagga gttactcaaa taacaattca ggattaaaaa
    56161 tagacagttg aggagcggaa ggtataaatt aagtttgctg ctgtataatg gatttatcac
    56221 taatactctt cgaaaatctc ttcaaaccac gtcagcttcg ccttgccgta gatatagtta
    56281 ctgacttcgt cagttctatc cacaacctca aaacagtgtt ttgagctgac ttcgtcagtt
    56341 ttatctgcaa cctcaaagct gtactttgag cagtctacgg ctagcttcct agtttgctct
    56401 ttgattttca ttgagtataa aagtgctcca ggccatattt tacacagcaa tagtcttcta
    56461 aaaatgaaca aaaaacattg taaaggctat caaaaaggag tataatgagt gcaagaaatt
    56521 tcggaggtga aagatgctag aagtaagaag tctagagaaa agttttggat ccaagcaagt
    56581 tttgtttggt attgactttc aagcgcgacc aggtcgtatt ttgggactag tcggaaaaaa
    56641 tggtgctgga aaaacaacga ttttccatag tattttaaaa ttcctagaat atcagggaga
    56701 aatcggtctg gatggtcagg atattcgtca ggagacctat gctcggattg gttatctgcc
    56761 tgaagaacgc agtctcatgc ctaaattgac agttcttgaa caagttcgct acttggcgac
    56821 tctaaaaggc atggatgcca aagaagtcaa agaaaaactc cctcaatgga tgaagaggtt
    56881 ggaagtgaaa ggaaagctgg cagataaaat caagagtttg tcaaaaggaa atcagcagaa
    56941 gattcagctc attattactc tgattcatga accagacctg attatcttgg atgagccttt
    57001 tagtggattg gacccagtta atacagaatt gctcaaacaa gtcatttttc aggaaaaaga
    57061 gcgcggagca accattatct tttctgacca tgtcatgacc aatgttgagg aactttgtga
    57121 cgatattctg atgatccgag atggccgtgt ggtcttgcac ggaccagttc aggatgtccg
    57181 caatcaatac gggaaaacac gtctctttgt ttcaagtgaa cgaagcaagg aagaattgga
    57241 aaatcttcct catgtcaaac aggtgagctt gaccaaacaa ggcagttgga aattgatttt
    57301 ggaggatgag agcgctggaa gggaactctt cccaatcttg actcaaggtc aatatatcgc
    57361 aacatttgac caacaagcgc caactatcga tgaaatcttt aaactagaat caggagtgga
    57421 agtatgagaa atatgtgggt tgtaatcaag gaaacctatc ttcgacatgt cgagtcatgg
    57481 agtttcttct ttatggtgat ttcgccgttc ctctttttag gaatctctgt aggaattggg
    57541 catctccaag gttcttctat ggctaaaaat aataaagtgg cagtagtgac aacagtgcca
    57601 tctgtagcag aaggactgaa gaatgtaaat ggtgttaact tcgactataa agatgaagca
    57661 agtgccaaag aagcaattaa agaagaaaaa ttaaaaggtt atttgaccat tgatcaagaa
    57721 gatagtgttc taaaggcagt ttatcatggc gaaacatcgc ttgaaaatgg aattaaattt
    57781 gaggttacag gtacactcaa tgaactgcaa aatcagctta atcgttcaac tgcttccttg
    57841 tctcaagagc aggaaaaacg cttagcgcag acaattcaat tcacagaaaa gattgatgaa
    57901 gccaaggaaa ataaaaagtt tattcaaaca attgcagcag gtgccttagg attctttctt
    57961 tatatgattc tgattaccta tgcgggtgta acagctcagg aagttgccag tgaaaaaggc
    58021 accaaaatta tggaagtcgt tttttctagc ataagggcaa gtcactattt ctatgcgcgg
    58081 atgatggctc tgtttctagt gattttaacg catattggga tctatgttgt aggtggtctg
    58141 gctgccgttt tgctctttaa agatttgcca ttcttggctc agtctggtat tttggttcac
    58201 ttgggagatg ctatctcact gaataccctg ctctttattt tgatcagtct tttcatgtac
    58261 gtagtcttgg cagccttcct aggatctatg gtttctcgtc ctgaggactc agggaaagcc
    58321 ttgtcgcctt tgatgatttt gattatgggt ggtttttttg gagtgacagc tctaggtgca
    58381 gctggtgaca atctcctctt gaagattggt tcttatattc cctttatttc gaccttcttt
    58441 atgccgtttc gaacgattaa tggctatgcg gagggagcgg aagcatggat ttcacttgct
    58501 attacagtga tttttgcggt ggtagcaaca ggatttatcg gacgcatgta tgctagtctc
    58561 gttcttcaaa cggatgattt agggatttgg aaaaccttta aacgtgcctt atcttataaa
    58621 tagaagagcc ttgcgaaagc gaggttttta aaggtgaaaa gggtggaatc acgaacctaa
    58681 ttatgtacac attatgtaaa caggttgctt gaaaactcaa ttgtatagtg aactgaatct
    58741 ggaataggat aatattactt ctaaaacatt cttataaatt gatttgaatt ccaaaatccc
    58801 atcgttccgt tcttatttca ttttgcgata tagcgaaagg aaataagtca gctcacaaaa
    58861 aacgcataat atcaaggatt tttaacactt gatactatgc gttttctaat tttaaagact
    58921 ttttgcctgg tctcttttgt tctatgagta tgttacatat ctaggtaaaa attatttaaa
    58981 gcaaattaac cttatttctc aagatgtagt aggttccgag ataaaagata gctatgaata
    59041 tgagttcttt aacaatggct atacctgctc ccatatagaa atggtcattg agtcctacca
    59101 gtgaattgac atagaaattg gaactaagat tgaagaaaag ttcaataaat ccaatgacga
    59161 tttggatacc aatgtaggct gcaacagcga gtgctgtacg gtattcattg aaaagctgtc
    59221 caatggaaat agccaggtag atgcagagga ttcctgaaat ggtatttagt aggaaggata
    59281 tacctgtaag aaagatctga gggagatgtg tttctacaaa tgtaatcaca taagaaagag
    59341 gaatccattc tggagctgtt aaagccagaa taataacagc acttagagcc aatacagcgg
    59401 tgctaatcaa tgaccagata aaggcaccga ttagtttggc tgtgatgata tggtgttcag
    59461 aaactggcaa ggtcaaagtc agatagcctt gtcggtcgta gacactacct ttgaagcgtt
    59521 taataatcaa gaaaatggtt gaaatcgcaa gtgtaagcat caagccacca aagactgtag
    59581 ctagaaaaag tagcatagta gcctgacttt cttggtaagg tagattttta aagccttgtg
    59641 tctgtattcc gatgagggca gaaaggacta gcacggctgc gtagagggct aaataccact
    59701 tgttaacatt tttaaattcg tagtgaacta aattccaaaa cataaataat ctcctttgct
    59761 taggccttaa attcctgacg gaagagttgg tcaatggatt cacctgactc gtagcgaata
    59821 tcatctacat ttccttgacg gacgactttt ccgtctttta ggaagacaat ttcatccaag
    59881 attggctcga tatcagaaat caagtgggta gaaatcaaaa cggtagaagt tggtgagtag
    59941 ttgttgataa tggtattgag gatataagca cgggctgctg gatccacccc accaatgggt
    60001 tcatccaaaa catagagacg agcatcacgg ctcataacca aaatcagttg aaccttttct
    60061 ttgtttcctt ttgatagttt cttgagacga ctattttcat caatgcccag gtctgcaagt
    60121 agatgatgag cgcgttcaag attgaaatct ttatagaagg tcttgaagta ggttagggct
    60181 tctttgacct tcatttgctc attgagatag gtcgtatcag gcaaataagc tacaacggcc
    60241 ttggttgctg ggcttgggtc catgtcgttg atgaggacac gtccttgatc tggttgtaag
    60301 aggccattaa ttagtttaat cagggttgtt tttcctgagc cgtttggccc aagaaggccg
    60361 acaatttttc cagctggaat gtcaagagaa acattttcaa gggctggtgt tgctccatat
    60421 gatttggata cattttcaaa tgctagtaat gacataggct taaactcctt taatataatc
    60481 actgactacg cctggtagtt cttctttttc atagccaaaa tgggtcatgg aggaaacgaa
    60541 gtgttccaat tcttcttctg ataattgttt gcgtgactgg gcgattagct ccttatcctt
    60601 agtcacaaat cgtccagttg ttcgcttgct gtagacaaat ccttctcgtt caaggtctga
    60661 taaggctctt tggatggtat tgggattgac accagcctcg ctagctagct ccctcacggt
    60721 tggaagttgt tgattgggtt ccagtgtatg ggaaacaatc tgaagcttga ttttctccat
    60781 aatctgtaaa tagatgggtt ttttgttgtc aaatgtccag gacatcttag tctcctttct
    60841 tttacccttt tgtcttaatt aaataataca acaaaacaaa aaacttgtca agcaaaaaga
    60901 agaattgttt tgggaatcgc ttaaaaaatg gtataatgaa aagaaaacga taaggaggga
    60961 aaggatgcgt tgtccaaaat gtggggctac caagtcaagt gttatcgata gtcgccaagc
    61021 agaagaaggg aacaccattc gtagaagacg tgagtgcgac gaatgccaac accgttttac
    61081 aacctacgaa cgagtagaag aaagaacctt agtggttgtt aaaaaagatg gcacacggga
    61141 acaattctcc agagataaaa tctttaatgg gattatccgc tcagcccaga aacgtcctgt
    61201 gtcaagtgat gaaatcaaca tggtagtcaa tcgtatcgaa cagaaactcc gtggtcgaaa
    61261 tgaaaatgaa attcaaagtg aggacattgg ttcactcgtc atggaggagt tggctgaatt
    61321 ggacgagatt acctatgtac gttttgctag tgtctatcgt agttttaagg atgtcagtga
    61381 gttagagagc ttgctccaac aaatcaccca gtcctctaaa aagaaaaagg aaagataaat
    61441 gaagccaatt gaccgttttt cttatctaaa gaataatcgg gtgtcgcaag atacctcatc
    61501 tctggtacag tgctacctcc cgattatcgg tcaggaggca ctgagccttt atctttatac
    61561 aatcagtttt tgggataatg gtagaaagga atatcttttt tcaagcattc tcaaccatct
    61621 taactttggg atggatagac tgataaaatc attgaaaatc ttatctgctt ttaatctctt
    61681 gaccctctat caaaaggggg atgtttatca gctagtcctc catgctcctc tatctagtca
    61741 agacttcttg gagcatcctg tttatcgcag actcttagag aaaaagattg gcgatgtagc
    61801 tgtggaggat ttgaaagttg aaagtgctga tggtgaagaa atacctgtct cactcaatca
    61861 agtctttcca gaattggcag aactaggcag tcaagaagac cttggtctca agaagaaagt
    61921 ggccaacgat tttgacttgg accattttcg ccagctgatg gctcgagatg gacttcgttt
    61981 tgcggatgag cagtccgatg tcttaaacct ctttgctatt gccgaggaga agaaatggac
    62041 ttggtttgaa acctatcaat tggccaagtc aacagctgtt tctcaggtta tttcaaccaa
    62101 acgcatgcgg gaaaaaattg ctcaaaaacc agtttcctct gactttagtc ttaaggaaac
    62161 aaccattatc aaagaagcca aaagtaaaac tgccctgcag ttcttggcag aaatcaagca
    62221 aacacgcaag ggaaccatta cccaaacaga aagagaactc ttgcaacaga tggctggctt
    62281 gggcttgctg gacgaagtca tcaatatcat tcttttattg acctttaata aggtcgattc
    62341 ggcaaatatc aatgagaaat atgccatgaa ggtagccaat gactatgcct atcaaaagat
    62401 tcattcggca gaaaaggcag tcttgcgcat ccgtgagcga ggacagaaag caaaaataca
    62461 aaaacagaat cagactgccc cagtaaaaac caatgttcct aaatggagta atcctgaata
    62521 taagaatgaa accagcgagg aaactcgtct ggaactagaa cgtaagaagc aagaactatt
    62581 agctcgatta gaaaaaggag gagattagat ggaaagtgtc ggagacgtac tcaaacgtca
    62641 acccagccgt ttttattatc aagatttggt ccagaaaatc atgaaggacc ctgatgttgc
    62701 ggcttttatc cagcaagaat ccttgactcc agaggaatta aatcgcagta tctccaagtt
    62761 taatcagtac atcaccgagc gtgacaagtt tctccgtggg gatacggatt atattgccaa
    62821 aggctacaag ccgattttgg ttaagaatca tggctatgca gatgtttcat atgaagaaac
    62881 tcctgagcta atcgcggctg aaaaagaagc ggctattaag aaccgtctca agttaatcaa
    62941 tctaccagcc agtctcaaga aagctagttt ggctcaagtt gacttggatg atttggggcg
    63001 cttaccagtt tttgaaaagc tactagcctt cgtggagcaa tatccagcta ttcgaaaagg
    63061 tctttactta tatggagact ttggtgtggg taaaagtttc atggtggcgg ccttagctca
    63121 tgatttatca gaaaaacgtg gtgtttcatc aactctcctc cactatccta gctttgtcat
    63181 tgatgtcaaa aatgctatca gtgatggcaa tgttaagacc ttggtggatg agattaagct
    63241 gtctgaggtc ctgattttag atgatattgg cgccgagcaa tcaaccgttt gggtgcgtga
    63301 cgaaatcctg caggtcattc tccaatatcg gatgcaggaa aatttaccga cctttttcac
    63361 atccaacttc aactttgaag atttggagaa gcattttgcg aaagtgaaac atggaaatga
    63421 cgaaacctgg gaagccagac gggtcatgga acgcatccgt tatttggctg aggagactcg
    63481 tttagaagga gtaaaccgtc gatgacagaa accattaaat tgatgaaggc tcatacttca
    63541 gtgcgcaggt ttaaagagca agaaattccc caagtagact taaatgagat tttgacagca
    63601 gcccagatgg catcatcttg gaagaatttc caatcctact ctgtgattgt ggtacgaagt
    63661 caagagaaga aagatgcctt gtatgaattg gtacctcaag aagccattcg ccagtctgct
    63721 gttttccttc tctttgtcgg agatttgaac cgagcagaaa agggagcccg acttcatacc
    63781 gacaccttcc aaccccaagg tgtggaaggt ctcttgatta gttcggtgga tgcggctttg
    63841 gctggacaaa atgccctgct ggcagctgaa agtttgggtt atggtggtgt gattattggc
    63901 ttggtacggt acaagtcaga agaagtggca gaactcttta acctgcctga ctacacctat
    63961 cctgtctttg ggatggcact aggtgttcca aatcaaactc atgatgtgaa accgagactg
    64021 ccactagata atgtagtctt tgaggaagaa taccaagaac agtcaactga ggcaatccaa
    64081 gcttatgacc gtgtgcaggc tgactatgct ggagcgcgtg cgaccacaag ctggagtcag
    64141 cgtctagcag aacagtttgg ccaagcagaa ccaagctcaa ctagaaaaaa tcttgaacag
    64201 aagaaattat tgtagaaagt gagaaattat ggccctacca actattgcca ttgtaggacg
    64261 tcccaatgtt gggaaatcaa ccctatttaa tcggatcgct ggtgagcgaa tctccattgt
    64321 agaagatgtc gaaggagtga cacgtgaccg tatctatgca acgggtgagt ggctcaatcg
    64381 ctcttttagt atgattgata caggaggaat tgatgatgta gatgctcctt tcatggaaca
    64441 aatcaagcac caggcagaaa ttgccatgga agaagcagat gttatcgttt ttgtcgtgtc
    64501 tggtaaggaa ggaattactg atgcagacga atacgtagct cgtaagcttt ataagaccca
    64561 caaaccagtt atcctcgcag tcaacaaggt ggacaaccct gaaatgagaa atgatatcta
    64621 tgatttctat gctctcggtt tgggtgaacc attgcctatc tcatctgtcc atggaatcgg
    64681 tacaggggat gtgctagatg cgatcgtaga aaatcttcca aatgaatatg aggaagaaaa
    64741 tccagatgtc attaagttta gcttgattgg tcgtcctaac gttggaaaat caagcttgat
    64801 caatgctatc ttgggagaag accgtgttat tgctagtcct gttgctggaa caactcgtga
    64861 tgccattgat acccacttta cagatacaga tggtcaagag tttaccatga ttgatacggc
    64921 tggtatgcgt aagtctggta aggtttatga aaatactgag aaatactctg ttatgcgtgc
    64981 catgcgtgct attgaccgtt cagatgtggt cttgatggtc atcaatgcgg aagaaggcat
    65041 tcgtgagtac gacaagcgta tcgcaggatt tgcccatgaa gctggtaaag ggatgattat
    65101 cgtggtcaac aagtgggata cgcttgaaaa agataaccac actatgaaaa actgggaaga
    65161 agatatccgt gagcagttcc aatacctgcc ttacgcaccg attatctttg tatcagcttt
    65221 aaccaagcaa cgtctccaca aacttcctga gatgattaag caaatcagcg aaagtcaaaa
    65281 tacacgtatt ccatcagctg tcttgaacga tgtcatcatg gatgccattg ccatcaaccc
    65341 aacaccgaca gacaaaggaa aacgtctcaa gattttctat gcgacccaag tggcaaccaa
    65401 accaccaacc tttgtcatct ttgtcaatga agaagaactc atgcactttt cttacctgcg
    65461 tttcttggaa aatcaaatcc gcaaggcctt tgtttttgag ggaacaccga ttcatctcat
    65521 cgcaagaaaa cgcaaataaa aaagtagaat ctggaatgac agttccggat ttttttgata
    65581 taataaaata atagaaaacg ctatcaaaag aaggggggat gtcggtgatg gaacatttgt
    65641 ttaaattctt acttttagca ccgtactttt attttgataa ctggattgaa aaggccaaca
    65701 gaaatagtaa gttttttcca attttttatt atttttactg gttttacatc cccttctatt
    65761 ctctttttag ccttgcttgg acagttgttt cagttctgtt tttcaatacc gtcttgagaa
    65821 atgtgacaga tatcaagtta tggggcattt ggtttctttt tattctgcta gctattggta
    65881 tgaattggtt aacttattcc tgtttcaaag aaatgtttcg cttgagacag gaactaggga
    65941 agtctaaggg tggaaggcat tgatttatat cggtttctat ttgtaggagg tggcttatgg
    66001 tacctctaaa atcatttatt acacgctatt ccaaggttta tattggtaat aacatttaat
    66061 agaacgaaac taaagaaaat aatttaaaag aggaggtttg tgatggaaca cttgtttaaa
    66121 atgataatct tattaccctg tttttacttt ttcagctgga ttgacaagga taatagagaa
    66181 agtaaatttt tcccaatttt ttactatttt tactggattt acatcaccct ctatgctctt
    66241 tttagccttg cttggacagt tttttcagtt ctgtttttca atatcgtctt gagaaatttg
    66301 acagatatca agttatgggg tatttggcta ttattactac ttatcgcttt tgctagtgac
    66361 tggttagact atatctgttt caaaaaaatg cttgacttga gacgggaact agggaagtct
    66421 aaaggcggaa ggcattgatt tatactcttc gaaaatctct tcaaaccacg tcaacgtcgc
    66481 cttggattat atatgtaact gacttcgtca gtcttatcta caacctcaaa gcagtgcttt
    66541 gagcaacttg cggctagttt cctagtttgc tctttgattt tcattgagta ttatattact
    66601 ttctatttgt aggaggtggc ttatgaagat tcctctctta actttggcaa ggcataaatt
    66661 tgtttatgtc ttgcttactt tgctttttct tgctttggtt tatcgtgatg ttttgatgac
    66721 ttatttcttt tttgatattc atgcgcccga tctagctaaa ttcgatggac aagcaattaa
    66781 aaatgactta ttaaaatcag cattagattt tcgtattctc cagttcaatc taggttttta
    66841 tcaatcattt attattccaa tcatcattgt tttgctaggt tttcaatata ttgagctgaa
    66901 aaataaagtt ttacgattga gtattggaag agaagtgagt tatcaagggt taaaaagaaa
    66961 gttgactttg caagttgcaa gtatcccttg tttgatatat ttagtgactg tgctgataat
    67021 tgcaatttta actcatttct ttgggacttt ttctcctctt ggatggaatt ctctattttc
    67081 tgatgggagt agtttacaaa ggctcctaga tggagagata acaagctatt tgttctttac
    67141 ttgtgtccta ctaatcggta ttttcatcaa tgcagtctat tttttacaaa tagttgatta
    67201 tttaggaaat gtgactcgtt cggcaatcac ctatttgatg tttctttggc ttggttctat
    67261 gctgctctat agtgccttgc cttactatat ggttcctatg acgagtttga tgcaagctag
    67321 ctatggggat gtaagtttga tgaaactctt tactccttat atcctttata ttgtctctta
    67381 catggtgctt gaaaaatatg aagataatgt ttaagaattt taacaatatt ttgctaaaga
    67441 gaaagattgt tttactactt cgtatagttc tgatgatgat tttgataaac catctattgt
    67501 caacagcggt tcaaaagcag gatgctgtta tctttttcaa gagagaattg atttcaattt
    67561 tttcctataa tgactattct gaagtgaatt tagaaatccc caaactcttg ttaaaccttt
    67621 cgcttttcat ggtaggatgg ctctctgtca ttttacttga aagtgatttg gcagaccatt
    67681 accatcactt gattcgctat caatcaagct cctttttcga ttatacaagg aaacgattgg
    67741 ttgtcatttc taaatttttt actcaagatt tgtttgtctg gtttcttggt ttacttcctc
    67801 taggaattca tttcaaaaca gtcgcacttt tctttttact tgctcagtta atgatgttgt
    67861 acttactact gtcttatctg atagcactga ttagtgcggg cgctggtttt tccttttttc
    67921 tctatttttt agcatttgtg ggacaagaat ggatgatgga tcatattgta acagtgtatt
    67981 tactactctt aagtttatta gttatgttga ttgttagtcg cttggaagag aaatttaaga
    68041 aaggataaac gatgagactt gaaattataa atggacagaa aatttatagg aaaagaccta
    68101 ttttaaatca gttgaatttg gtgtttcaat caggaaaaat ttatggactt aaaggtgata
    68161 atggatctgg caagacggtt cttttaaaga tacttgctgg ttatattaag cttgacaaag
    68221 gaaaagttct tcaagatggt aaagtttacg gggtaaaaaa tcattatatt caggatgcag
    68281 gaattttaat tgaaaaagtc gagtttttat ctcatttatc cctgagagaa aatttggaac
    68341 tgttaaggta tttttcatct aaagttacgg aaaaaagaat tgcctattgg attcaatact
    68401 atgatttaca ggaatttgaa gacattgaat accgtcattt atccttagga acaaagcaaa
    68461 aaatggcctt gattcaagcc tttatttcct ctccttctat actctttctc gatgaaccta
    68521 tgaatgcttt ggatgagaag agtgtgaggt taaccaaaca ggtcatttta tcttacctga
    68581 aaaaagaaaa tggtctggtt atcctgacgt cgcacatatc ggaagatatt tcagaccttt
    68641 gtacagatgt attagttgtc gaaaatggac atatacaaat gtaaaggata tacaatccta
    68701 ggagatggct tatggcacat ctaaaatcat ttattacacg ctattccaag gtttatattg
    68761 gtttagttct gctgatctgg ctgtctttct tctttatccc ttgggataaa ccacttctgg
    68821 ggataaggat tgacatcttc atcatacaga aaatcttgct agcttttgga attctgtcca
    68881 ttctcatggc cttgctgtcc aagaaagtca gtctctttgt ttttggactg atttgctgtc
    68941 tttctctttg gattaactta tttatcacac ttgccatttt gccgattttt ggcaattaaa
    69001 cagtcataaa agtcggagag gttagcttga aaactaacct ctttttcctt ttcaaaatgg
    69061 ggattcttcc ttgaaaataa tcagtaattg tgctaaaatt aaaggaacat tctaaaatat
    69121 tcggaattta aagtaaggaa aaacatggct aatattttaa aaacaattat cgaaaatgat
    69181 aaaggagaaa tccgtcgtct ggaaaagatg gctgacaagg ttttcaaata cgaagaccaa
    69241 atgactgctt tgactgacga ccaactaaaa gcaaaaacag ttgaatttaa ggaacgttat
    69301 caaaatggag aatcactgga ttcattgctt tacgaagcat ttgcggttgt ccgtgaaggt
    69361 gccaaacgtg tcctaggtct cttcccttat aaggttcagg tcatgggggg gattgttctt
    69421 caccatggtg acgtgccaga gatgcgtaca ggggaaggga aaaccttgac tgcgaccatg
    69481 ccggtatacc tcaatgccct ttcaggtaaa ggggttcacg tagttacggt taatgaatac
    69541 ctgtcagaac gtgacgcgac tgagatgggt gaattgtact cttggcttgg tttgtcagta
    69601 gggattaact tggctaccaa atctccaatg gagaaaaaag aagcctatga gtgtgatatt
    69661 acttactcaa ctaactcaga aatcggattt gactaccttc gtgacaacat ggtcgttcgc
    69721 gctgaaaaca tggtacaacg tccgcttaac tatgccttgg tcgatgaggt tgactctatc
    69781 ttgattgacg aggctcgtac acctttgatt gtatcaggtg ccaatgcggt tgaaaccagt
    69841 cagttgtatc acatggcaga ccactatgta aaatctttga acaaagatga ctacatcatc
    69901 gatgtgcagt ctaagactat tggtttgtct gattcaggga ttgacagggc tgaaagctac
    69961 ttcaagcttg aaaacctcta tgacatcgaa aacgtggctt tgacccactt tatcgataac
    70021 gcccttcgtg ccaactacat catgcttctc gatattgact atgtggtgag cgaagagcaa
    70081 gaaatcttga ttgtcgacca atttacaggt cgtaccatgg aaggtcgtcg ttattctgat
    70141 ggattgcacc aagctattga agccaaagaa ggtgtgccaa tccaggatga aaccaagaca
    70201 tctgcctcaa tcacgtacca aaaccttttc cgtatgtaca agaaattgtc tggtatgacg
    70261 ggtacaggta agactgagga agaagaattc cgtgaaatct acaacattcg tgttattcca
    70321 atcccaacaa accgtcctgt tcaacgtatt gaccactcag accttcttta tgcaagtata
    70381 gaatctaagt ttaaagcggt tgtcgaagac gttaaggctc gttaccaaaa gggtcaacct
    70441 gtcttggttg gtacagtagc ggttgaaact agtgactaca tttctaagaa attggttgca
    70501 gctggtgttc ctcacgaagt cttgaatgcc aaaaaccact atagagaagc ccaaatcatc
    70561 atgaatgctg gtcaacgtgg tgccgttacc atcgcaacca acatggcggg tcgtggtacc
    70621 gacatcaagc ttggtgaagg tgttcgtgaa cttggaggac tttgtgttat tggtacagaa
    70681 cgtcatgaaa gtcgtcgtat cgataaccag cttcgtggac gttcaggtcg tcaaggagat
    70741 ccaggtgagt cacaattcta cctatctctt gaagatgatt tgatgaaacg ttttggttct
    70801 gaacgcttga agggaatctt tgaacgcttg aacatgtctg aagaggcgat tgagtctcgc
    70861 atgttgacgc gtcaggttga agcagctcag aaacgtgtcg aaggaaataa ctacgatacc
    70921 cgtaaacaag tccttcaata cgatgatgtc atgcgtgaac aacgtgagat tatctatgct
    70981 caacgttacg atgtcatcac tgcagatcgt gacttggcac ctgaaattca gtctatgatc
    71041 aaacgcacga ttgaacgtgt cgttgatggt catgcgcgtg ccaaacaaga tgaaaaacta
    71101 gaggcaattt tgaactttgc taagtacaac ttgcttcctg aagattctat tacgatggaa
    71161 gacttgtcag gcttgtctga taaggccatc aaggaagagc ttttccaacg tgccttgaag
    71221 gtttacgata gtcaggtttc aaaactacgc gatgaagaag cagttaaaga attccaaaaa
    71281 gttttgattc tacgagtagt ggataacaag tggacagatc atatcgatgc ccttgatcaa
    71341 ttgcgtaacg cggttggact tcgtggctat gctcagaaca accctgttgt tgagtatcag
    71401 gcagaaggtt tccgtatgtt taatgatatg attggttcga ttgagtttga tgtgacacgc
    71461 ttgatgatga aagcacaaat tcatgaacaa gaaagaccac aggcagaacg tcatatcagt
    71521 acaacagcga ctcgcaatat cgctgctcac caagcaagta tgccagaaga tttggatttg
    71581 agccagattg gacgcaatga actttgccca tgtggttctg gtaagaaatt taaaaactgt
    71641 cacggtaaaa gacaataaaa tgagatagtt tagaggcgga tatcttgtga aaagtaaatt
    71701 tttactgggt atccgtttgc tttataagga gatgagttat ggtatttaca gcaaaaagct
    71761 ctaaaataaa tatagaagaa gttcgtgcct tgtcaaaatt agaaggtcag gctttggaga
    71821 ggaaatcaca gcgagatcaa gagctagaag ccattatacg tggagaagac cagcgaattc
    71881 tcttggtaat cgggccatgc tcatctgaca acgaagaagc tgtccttgaa tacgctaagc
    71941 gtttggcagt cctacaagaa gaagtggcag atcgtatctt tatggttatg cgtgtttata
    72001 ctgccaaacc ccgtaccaac ggagatggct ataagggctt gattcaccag cctaacgcga
    72061 cagaagcgcc tagtcttatc aatggaatca aagccgttcg ccatcttcac tatcgtgtca
    72121 tcacagaaac agggatgaca actgctgatg aaatgcttta tcctgaaaac cttccgcttg
    72181 tagatgattt gatttcttac atggcagttg gtgcccgttc agttgaagac cagcaacacc
    72241 gctttgtggc aagtggggca ggattttcta ctggttttaa aaatccaacc tctggaaatc
    72301 tcaatgtcat gtttaatggg atttatgctg ctcaaaacaa acaaagtttc cttttcttag
    72361 gaaaagaagt agaaacaact gggaacccgc tttcacacgc tattcttcgt ggtgctctta
    72421 atgagtatgg aaaaaatatt cccaactact attatgacaa tttaattgat accattgccc
    72481 agtatgagaa aatgggcttg gaaaatcctt ttatcatcat tgataccaat catgacaatt
    72541 ctggtaagca gtatattgaa cagatccgaa ttgtccgcca gaccttgatt aaccgtgctt
    72601 ggaatgaaaa aattaagcag ttcgttcgtg gttttatgat tgagtcttat ctggaagatg
    72661 gtcgacaaaa tgagccagaa gtatttggta agtctatcac agacccttgc ctgggttggg
    72721 ataacacaga agctcttgtc agagaaattt acaaaacgtt aggagaataa gatggcattt
    72781 attgaaaaag gtcaagaaat cgatatggaa gtcatcaagg ctgaaaccca attgtctgcg
    72841 gaagccttga gactcaagga aagccgtgac agggaattgg cagatattat ttcaggggaa
    72901 gatgaccgta ttctcttggt gattggtcct tgctcttctg ataatgaaga ggcggtcttg
    72961 gaatatgctc gccgtttatc cgccttgcaa aagaaggtag cggacaagat tttcatggtc
    73021 atgcgcgtgt atactgctaa gcctcgtacc aatggagacg gctataaagg attagttcac
    73081 cagccagata cttctaaggc tccaagcctg attaatggct tgcaggctgt gcgccagttg
    73141 cactaccgcg tgattacaga gactggtttg acaacggcag atgagatgct ttatccgtca
    73201 aatctgatct tggtggatga cttggtcagc taccatgccg ttggagctcg ttctgtggaa
    73261 gaccaagagc accgctttgt ggcttctggg attgatgcac cagtagggat gaaaaatcca
    73321 acctcaggaa atttgggtgt tatgtttaac gccatctatg ctgctcaaaa caagcaaacc
    73381 ttcctttatc atgggcagga agttgagaca tcaggtaatc ctttggccca tgttatcctc
    73441 cgtggagcag tcaacgagta tggcaattat atgccgaatt actactatga aaacctactc
    73501 caagccattg aacgctatga aaccatggga cttgaaaatc cttttatcct cattgacacc
    73561 aaccatgata actcaggcaa gcaatatatg gagcagattc gaattgttcg ccagaccttg
    73621 cagaatcgtg attggaatga gaaaattaaa aagacggttc gaggatttat gattgaatct
    73681 tacctagcag atggtcgtca aaaccaacca gagatctttg gttgctctat tactgaccct
    73741 tgcctaggtt gggaaaatac agaggccttg gtagaagaga tttatgttac cttgacaaaa
    73801 taagtgaaaa ggatggagtt ggggaatctc aactcctttt gatgagaatg atagttggac
    73861 acggaattga catcgaagaa ttggcttcga tagaaagtgc agttacacga catgaaggat
    73921 ttgctaagcg tgtactgacc gctcaggaaa tggagcgctt caccagtctc aaaggacgca
    73981 ggcaaataga atatttagct ggtcgctggt cggctaagga ggccttttcc aaggctatgg
    74041 gaacgggcat tagcaagctc ggttttcagg atttggaagt cttgaacaat gaacgtgggg
    74101 cgccttattt tagtcaggca ccattttcag gaaagatttg gctgtctatc agccacaccg
    74161 atcagtttgt gacagccagt gtcattttgg aggaaaatca tgaaagctag tccacataga
    74221 ccaaccaagg ctctgattca tctgggagct attcgacaaa atattcagca aatgggggct
    74281 catatccctc aaggaacgct caagttggct gtggttaagg ccaatgctta tggtcatgga
    74341 gctgttgccg ttgccaaggc aattcaagat gatgttgatg gcttttgcgt ttccaatatc
    74401 gatgaagcca ttgaactcag acaagctgga ctcagcaagc caattctcat tttaggagtt
    74461 tctgaaatcg aagctgttgc tctagctaaa gaatatgact tcaccttgac agtggctgga
    74521 ctggagtgga ttcaagcact cttagataag gaagtggacc taactggatt gacagtccac
    74581 ctcaagattg attcagggat gggacggatt ggttttagag aggccagtga ggttgagcag
    74641 gctcaagatt tgctccaaca acacggtgtt cgtgttgaag gaatctttac ccactttgct
    74701 actgctgatg aggaatcaga tgactatttt aatgcccagt tagaacggtt taaaactatt
    74761 ttagctagta tgaaggaagt tccagagctg gttcatgcta gcaattctgc aacgactctt
    74821 tggcatgtag agactatttt caatgcggtt cgtatgggag atgccatgta tggcctcaat
    74881 ccaagtggag cggtcttgga tttgccttat gatttgatac cggccttgac cttggagtct
    74941 gctctggttc atgtcaagac agttccatct ggagcttgca tgggatatgg agcaacttat
    75001 caagcggata gcgagcaagt catcgcgacc gtgccaatcg ggtatgcaga tggatggaca
    75061 agagacatgc aaaatttctc tgtcttggta gatggccaag cttgcccaat tgtcggcagg
    75121 gtttcgatgg accaaatcac tattcgattg cctaagcctt acccactcgg aaccaaggta
    75181 accttgattg gctccaatgg ggataaggaa atcactgcaa ctcaggtagc gacctaccgc
    75241 gtaaccatta actatgaggt ggtttgcctc ctcagcgacc gtattccgag agaatattat
    75301 tagaaaagaa aggagtggag catgaatcta catcaaccct tgcatgtctt gcctggtgtg
    75361 ggaccaaagt cagcagaaaa atacgccaaa ctaggaattg aaaacttgca agatctcttg
    75421 ctctactttc ctttccgtta tgaagacttc aaaaccaagc aggtgctgga gctggaagac
    75481 ggtgagaagg cagttctttc tggtcaggta gtgactcctg ctagtgtcca gtattatggt
    75541 ttcaagcgca atcgcctgcg ttttagtctc aagcagggag aggtcgtttt tgcggtgaat
    75601 ttctttaacc agccctatct ggctgataaa atagagttgg gagcaaccct tgctgtcttt
    75661 ggaaaatggg accgcgctaa ggctagtctg actgggatga aggttctggc tcaggtagaa
    75721 gatgacctcc agcctgttta tcgtctggct cagggaatca gtcaggccag tctggttaag
    75781 gtcatcaaga cggcttttga tcagggactg gacctcttga tagaagaaaa tctgccccag
    75841 tctttactag acaaatacaa actcatgtcc cgttgtcagg cagtccgagc tatgcatttt
    75901 ccaaaggatt tggcagaata caagcaggct cttcgccgta taaagtttga ggaactcttt
    75961 tatttccaaa tgcagctgca gacgctcaag tctgaaaata gaattcaggg aagtggtctg
    76021 gttctggatt ggtctcagga aaaagtgaca gcagttaaag caagtcttcc ttttgccctg
    76081 acccaagctc aggaaaagag tttgcaggaa attttaactg atatgaagtc cgaccaccac
    76141 atgaatcgtc tcctacaagg ggatgtgggg agtggaaaaa cggtagtcgc tggcttggcc
    76201 atgtttgcgg cagtgacagc aggttatcag gctgccctaa tggtaccaac agaaatcctc
    76261 gcagagcaac actttgagag tttacagaac ctttttccca atttgaaact ggctctcttg
    76321 acaggttcct tgaaagctgc agaaaagaga gaagtcttgg agaccattgc caagggtgag
    76381 gctgatttga ttataggaac tcacgctctg atacaagatg gggtggagta tgctcgtctt
    76441 ggtttgatta ttatcgatga gcagcaccgt tttggtgtag ggcaaaggcg tattttacgg
    76501 gaaaaaggtg acaatccaga tgtcctcatg atgacggcga ctcccattcc acggacgctt
    76561 gccatcacag cctttggaga tatggatgtt tccattatcg accagatgcc agcaggtcgg
    76621 aagcctattg tgacgcgctg gatcaaacat gagcaactac ctcaggtctt gacttggtta
    76681 gagggggaaa ttcaaaaagg ttcccaagcc tatgtcatct ctcctttgat tgaagaatca
    76741 gaagctctag atttgaaaaa tgccattgcc ttatcagaag agctgacggc tcattttgca
    76801 ggcaaggcag aggtggctct tctacatggt aggatgaaga gtgacgaaaa agaccagatc
    76861 atgcaggatt tcaaggagag aaagacggat attctggttt cgacgacggt tattgaggtt
    76921 ggggtcaacg ttcccaatgc gactgtcatg attatcatgg atgccgatcg cttcgggctc
    76981 agccagcttc accagcttag aggtcgtgtc ggtcgggggg acaagcagtc ctacgctgtt
    77041 ctcgttgcca atcccaagac ggattctggg aaagaccgca tgcgcatcat gacagaaacg
    77101 accaatggat ttgtccttgc ggaggaagat ttgaaaatgc gtggttctgg tgagattttt
    77161 ggaaccagac agtcaggact tccagagttc caagtggctg atattatcga agattttccg
    77221 attttagaag aagcaagaaa ggttgctagc tacattagtt ctatagaagc ttggcaagaa
    77281 gatccagagt ggcgcatgat tgcccttcat ctggaaaaga aagaacatct ggattaagct
    77341 ttctctaagg aaaacttaag ctagttttta ggtttggctc ttatactaaa gtcatcaaaa
    77401 agaaacgagg actctcatat gacagtaacg attaaagtaa attaccaaac cactttccaa
    77461 aagaaggaag caaaaaacta gtataaacag aagagagagc gaaatgctct ttttcgtttc
    77521 taaaactact ttcagcccat catcctaaaa gtaaagaatc taaattcact ttctatttat
    77581 ccttctttct tgcattgatt acatagatat gctacagttg tggtaacgat tacaaaataa
    77641 aaggagcatg ctatgaaaaa tccagctttg ctagaagaaa ttaagaccta tagaggaagg
    77701 gatgaggttc cggaagactt tgatgctttc tgggatgggg aagtgaaaaa tgtttccacg
    77761 cttccatcct accacttgga ggaaagagat ttccacattc ctcaagtcaa gtgctatgag
    77821 ttaacatttg aaggaagcaa ggaaggaaag gtctatgcac gcattgttct tccaaagagt
    77881 gaggagaagg ttccactaat cttccatttt catggttata tgggacgtgg ctgggactgg
    77941 gccgacatgc tgggcttcac cgtagctggt tacggtgttg tttccatgga tgtgcggggc
    78001 cagtcaggtt actcacaaga cggcttgcgt tctcctttag gaaataccgt gaaggggcat
    78061 attatccgtg gtgctatgga aggtcgggac cacctctttt ataaggatgt ttatctggat
    78121 atttaccagt tggtcgaaat tgttgctagt ctgtctcagg ttgatgagaa gcgtctttct
    78181 agctatggtg cctcacaagg aggggctcta gctctagttg cagcagcgct caatcctcga
    78241 attcagaaaa cagttgccat ttatcccttc ttgtcagact tcagacgggt gattgagatt
    78301 ggtaatacta gcgaggctta cgacgaactt ttccgctatt tcaagtttca cgaccccttc
    78361 catgaaacag aggaggaaat catggcgacc cttgcctata tcgatgtcaa aaatcttgcc
    78421 catcgtatcc aaggtgaggt taagatgatt acgggcttgg acgacgatgt ttgctatccc
    78481 attacccagt ttgcgattta taatcgtctg acctgcgata aaacctatcg catcatgcct
    78541 gagtatgctc acgaagccat gaatgtattt gtcaatgacc aagtctacaa ctggctctgt
    78601 ggaagtgaga ttccttttaa atatctaaaa taaggagtcg actctaagca caaaatctta
    78661 aaaattacaa acacgcatag tatcagggga ttaaaaaaac ttgatactat gcgttttatc
    78721 atggaaatat agtaaaatga aacaagaata ggacaaaacg atcaggacag tcaaatcgat
    78781 ttctaacaat gttttagaag caaatgtgta ctattctagt ttcaatctat tatatttata
    78841 gaattttttg ttgctagatt tgtcaaattg cttaaaataa tttttttcag aaagcaaaag
    78901 ccgataccta tcgagtaggg tagttcttgc tatcgtcagg cttgtctgta ggtgttaata
    78961 cttttcaaaa atctctttaa accacgtcag cttcgccttg ccgtatatat gttactgact
    79021 tcgtcagttc tatctgccac ctcaaaacgg tgttttgagc tgacttcgtc agttctatct
    79081 gccacctcaa aacggtgttt tgagctgact ttgtcagtct tatctacaac ctcaaaacag
    79141 tgttttgagc atcatgcggc tagcttctta gtttgctctt tgattttcat tgagtataaa
    79201 aacagatgag tttctgtttt ctttttatgg actataaatg ttcagctgaa actactttca
    79261 aggacattat tatataaaag aattttttga aactaaaatc tactatatta cactatattg
    79321 aaagcgtttt aaaaatgagg tataataaat ttactaacgc ttataaaaag tgatagaatc
    79381 tatttttatg tatatgtaaa gatagattgc tgtaaaaata gtagtagcta tgcgaaataa
    79441 cagatagaga gaagggattg aagcttagaa aaggggaata atatgatatt taaggcattc
    79501 aagacaaaaa agcagagaaa aagacaagtt gaactacttt tgacagtttt tttcgacagt
    79561 tttctgattg atttatttct tcacttattt gggattgtcc cctttaagct ggataagatt
    79621 ctgattgtga gcttgattat atttcccatt atttctacaa gtatttatgc ttatgaaaag
    79681 ctatttgaaa aagtgttcga taaggattga gcaggaagta tggtgtaaat agcataggct
    79741 gatgtccatc atttgcttat aaagagatat tttagtttaa ttgcagcggt gtcctggtag
    79801 ataaactaga ttggcaggag tctgattgga gaaaggagag gggaaaattg gcaccaattt
    79861 gagatagttt gtttagttca tttttgtcat ttaaatgaac tgtagtaaaa gaaagttaat
    79921 aaaagacaaa ctaagtgcat tttctggagt aaatgtctta tttcagaaat cgggatatag
    79981 atatagagag gatcagtatg aatcggagtg ttcaagaacg taagtgtcgt tatagcatta
    80041 ggaaactatc ggtaggagcg gtttctatga ttgtaggagc agtggtattt ggaacgtctc
    80101 ctgttttagc tcaagaaggg gcaagtgagc aacctctggc aaatgaaact caactttcgg
    80161 gggagagctc aaccctaact gatacagaaa agagccagcc ttcttcagag actgaacttt
    80221 ctggcaataa tcaagaacaa gaaaggaaag ataagcaaga agaaaaaatt ccaagagatt
    80281 actatgcacg agatttggaa aatgtcgaaa cagtgataga aaaagaagat gttgaaacca
    80341 atgcttcaaa tggtcagaga gttgatttat caagtgaact agataaacta aagaaacttg
    80401 aaaacgcaac agttcacatg gagtttaagc cagatgccaa ggctccagca ttctataatc
    80461 tcttttctgt gtcaagtgct actaaaaaag atgagtactt cactatggca gtttacaata
    80521 atactgctac tctagagggg cgtggttcgg atgggaaaca gttttacaat aattacaacg
    80581 atgcaccctt aaaagttaaa ccaggtcagt ggaattctgt gactttcaca gttgaaaaac
    80641 cgacagcaga attgccaaat ggtcgagttc gcctgtatgt taatggtgtt ttatctcgaa
    80701 caagtctgaa atctggcaat ttcattaaag atatgccaga tgtaacgcat gtgcaaatcg
    80761 gagcaaccaa gcgtgccaac aatacggttt gggggtcaaa tctacagatt cggaatctca
    80821 ctgtgtataa tcgtgcttta acaccagaag aggtacaaaa acgtagtcaa ctttttaaac
    80881 gctcagattt agaaaaaaaa ctacctgaag gagcggcttt aacagagaaa acggacatat
    80941 tcgaaagcgg gcgtaacggt aaaccaaata aagatggaat caagagttat cgtattccag
    81001 cacttctcaa gacagataaa ggaactttga tcgcaggtgc agatgaacgc cgtctccatt
    81061 cgagtgactg gggtgatatc ggtatggtca tcagacgtag tgaagataat ggtaaaactt
    81121 ggggtgaccg agtaaccatt accaacttac gtgacaatcc aaaagcttct gacccatcga
    81181 tcggttcacc agtgaatatc gatatggtgt tggttcaaga tcctgaaacc aaacgaatct
    81241 tttctatcta tgacatgttc ccagaaggga agggaatctt tggaatgtct tcacaaaaag
    81301 aagaagccta caaaaaaatc gatggaaaaa cctatcaaat cctctatcgt gaaggagaaa
    81361 agggagctta taccattcga gaaaatggta ctgtctatac accagatggt aaggcgacag
    81421 actatcgcgt tgttgtagat cctgttaaac cagcctatag cgacaagggt gatctataca
    81481 agggtaacca attactaggc aatatctact tcacaacaaa caaaacttct ccatttagaa
    81541 ttgccaagga tagctatcta tggatgtcct acagtgatga cgacgggaag acatggtcag
    81601 ctcctcaaga tattactccg atggtcaaag ccgattggat gaaattcttg ggtgtaggtc
    81661 ctggaacagg aattgtactt cggaatgggc ctcacaaggg acggattttg ataccggttt
    81721 atacgactaa taatgtatct cacttaaatg gctcgcaatc ttctcgtgtc atctattcag
    81781 atgatcatgg aaaaacttgg catgctggag aagcggtcaa cgataaccgt caggtagacg
    81841 gtcaaaagat ccactcttct acgatgaaca ataaacgtgc gcaaaataca gaatcaacgg
    81901 tggtacaact aaacaatgga gatgttaaac tctttatgcg tggtttgact ggagatcttc
    81961 aggttgctac aagtaaagac ggaggagtga cttgggagaa ggatatcaaa cgttatccac
    82021 aggttaaaga tgtctatgtt caaatgtctg ctatccatac gatgcataat ggaaaagaat
    82081 acattatcct cagcaatgca ggtggaccga atcgtgaaaa tgggatggtc cacttggcac
    82141 gtgtcgaaga aaatggtgag ttgacttggc tcaaacacaa tccaattcaa aaaggagagt
    82201 ttgcctataa ttcgctccaa gaattaggaa atggggagta tggcatcttg tatgaacata
    82261 ctgaaaaagg acaaaatgcc tataccctat catttagaaa atttaattgg gaatttttga
    82321 gcaaagatct gatttctcct accgaagcga aagtgaagcg aactagagag atgggcaaag
    82381 gagagatggg caaaggagtt attggcttgg agtttgactc agaagtattg gtcaacaagg
    82441 ctccaaccct tcaattggca aatggtaaaa cagcgacttt cctaacccag tatgatagca
    82501 aaaccttgtt gtttgcagta gataaggaag atatcggaca ggaaattatt ggtatagcta
    82561 aaggaagcat cgaaagtatg cataatcttc ctgtaaatct agcaggtgcc agagttcctg
    82621 gcggagtaaa tggtagcaaa gcagcggtgc atgaagttcc agaatttaca gggggagtta
    82681 atggtacaga gccagctgtt catgaaatcg cagagtataa gggatctgat tcgcttgtaa
    82741 ctcttactac aaaagaagat tatacttaca aagctcctct tgctcagcag gcacttcctg
    82801 aaacaggaaa caaggagagt gacctcctag cttcactagg actaacagct ttcttccttg
    82861 gtctgtttac gctagggaaa aagagagaac aataagagaa gaattctaaa catttgattt
    82921 tgtaaaaata gaaggagata gcaggttttc aagcctgcta tctttttttg atgacattca
    82981 agctgatacg aaatcataag aggtctgaaa ctactttcag agtagtctgt tctataaaat
    83041 atagtagatt gaaataagat gtgaacaact ctatcaggaa agtcaaatta atttatagaa
    83101 aaattttagc agtcaaggtg tactgttata gattcaatat attatacgac tattaacctt
    83161 atcttctcct aaaattgact ttcttgtttt cttatcttgt ccactcgaaa caagtattgt
    83221 aagaatttga ttatttttga aagtactttt aatatacttg atatagttaa aaaagatttg
    83281 aaactaaatt ccaaattaga aaaagacttg aaatactaaa aaaaaaaagt atactctaat
    83341 tgaaaacggt aacaaaacta atttagagaa tgaaatatag agtatttctc tcttaaaagt
    83401 ttttggtgaa acgagatgta gaaaggagat ttagccaaag agtctattag tgctagaata
    83461 atagattaga attattttag aaaaacgaag tgagcagctt ataaattcaa gtccccaaat
    83521 agactcatac tagtatcttt tgcaaaaaat aaagggcgac ttccttcatg aatatcaatt
    83581 tcatctataa ggaaggtagc taattgaact aacttattta ttctgtttgt cgctagaaaa
    83641 atcagacctc cttgtgaaga ttgaggagat acttaatgaa aatcaaagaa gaaactagca
    83701 agctagtagc agattgccca aaacaccgct ttgaggttgt agataagact gacctatata
    83761 atccaaggtg aagcgactgt ggtttgaaga gattttcaaa gagtatagac tagagagtag
    83821 tgtttttatg tccttctagt agaaaatgct agacagaaga atggggaact tggataggaa
    83881 aaatagattg agaaaggagg ttagaagaga tgattattac aaaaattagc cgtttaggaa
    83941 cttatgtggg agtcaatcca cattttgcaa cattaataga ttttctagaa aaaacaggac
    84001 tagaaaattt aacagaaggt tcgattgcta tcgatggtaa tcgattgttt gggaattgct
    84061 ttacttatct agcagatggt caagcagggg atttctttga aacacaccaa aaatatttgg
    84121 atattcattt agttttggaa aacgaagaag ccatggctgt tacatcgccg gaaaatgtaa
    84181 gcgttaccca agaatatgat gaagagaaag atattgaatt atacacaggg aaagtggaac
    84241 agttggttca tttgagagct ggtgaatgcc tcatcacttt tccagaagat ttacatcaac
    84301 ccaaggttcg tataaatgat gaacctgtga aaaaagttgt ctttaaagtt gcgatttctt
    84361 aatgtagaaa gagaagaacg atgaaaaaaa tgagaaagtt tttatgtcta gctggaattg
    84421 cgctagcggc tgttgccttg gtagcttgtt caggaaaaaa agaagctaca actagtactg
    84481 aaccaccaac agaattatct ggtgagatta caatgtggca ctcctttact caaggacccc
    84541 gtttagaaag tattcaaaaa tcagcagatg ctttcatgca aaagcatcca aaaacgaaaa
    84601 tcaagattga aacattttct tggaatgact tctatactaa atggactaca ggtttagcaa
    84661 atggaaatgt gccagatatc agtacagctc ttcctaacca agtaatggaa atggtcaact
    84721 cagacgcttt ggttccgcta aatgattcta tcaagcgtat tggacaagat aaatttaacg
    84781 aaactgcctt aaatgaagca aaaatcggag atgattacta ctctgttcct ctttattcac
    84841 atgcacaagt catgtgggtt agaacagatt tgttaaaaga acataatatt gaggttccta
    84901 aaacttggga tcaactctat gaagcttcta aaaaattgaa agaagctgga gtttatggct
    84961 tgtctgttcc gtttggaaca aatgacttaa tggcaacacg tttcttgaac ttctacgtac
    85021 gtagtggtgg aggaagcctc ttaacaaaag atcttaaagc agacttgaca agccaacttg
    85081 ctcaagatgg tattaaatac tgggttaaat tgtataaaga aatctcacct caagattctt
    85141 tgaactttaa tgtccttcaa caagctacct tgttctatca aggaaaaaca gcatttgact
    85201 ttaactctgg cttccatatc ggaggaatta atgccaacag tcctcaattg attgattcga
    85261 ttgatgctta tcctattcca aaaatcaaag agtctgataa agatcaagga attgaaacct
    85321 caaacattcc aatggttgtt tggaaaaatt caaaacatcc agaagttgct aaagcattct
    85381 tagaagcact ttataatgaa gaagactacg ttaaattcct tgattcaact ccagtaggta
    85441 tgttgccaac tattaagggg attagcgatt ctgcagccta taaagaaaat gaaactcgta
    85501 agaaatttaa acatgctgaa gaagtaatta ctgaagctgt taaaaaaggt actgctattg
    85561 gttatgaaaa tggaccaagt gtacaagctg gtatgttaac taaccaacac attattgaac
    85621 aaatgttcca agatatcatt acaaatggaa cagatcctat gaaagcagca aaagaagcag
    85681 aaaaacaatt aaatgattta tttgaggctg ttcagtagat gtaaaagact agaaaatagg
    85741 tgggctagtg agctgaaaag ctctagccca atcttgtaaa agaagggaga aggagaatgg
    85801 ttaaagaacg taatttaact cgctggatat ttgttttgcc agctatgatt atcgtaggat
    85861 tactctttgt ttatccgttt ttctcgagta ttttttatag ctttaccaat aagcatttga
    85921 ttatgcctaa ttataaattt gttggtttgg ctaactataa agctgtgcta tcagatccca
    85981 acttctttaa tgcgttcttt aattcaatta agtggaccgt tttctcatta gttggtcaag
    86041 ttttagtagg gtttgtattg gctttagctc ttcacagagt acgccacttc aagaaattat
    86101 ataggacctt attgattgtt ccttgggcat ttcctaccat cgttattgcc ttttcttggc
    86161 agtggattct aaacggggtt tatggctact tacctaatct aatcgtaaaa ttaggtttaa
    86221 tggaacatac acctgcattt ttgacagata gtacatgggc attcctatgt ttggtgttta
    86281 tcaacatttg gtttggagca ccaatgatta tggttaatgt gctttcagct ttgcaaacag
    86341 taccagaaga acaatttgag gctgctaaga tagatggtgc ttcaagttgg caggtgttca
    86401 agtttatcgt ctttccacat attaaagtgg ttgtaggact tctagttgtt ttgagaactg
    86461 tatggatctt taataacttt gacattatct acctcattac tggtggtgga ccagccaatg
    86521 ctacaacgac gcttccaatt tttgcttaca acctgggctg gggaactaaa ttgttgggtc
    86581 gtgcttcagc agttacagta ttgctcttta tcttcttggt ggcgatttgc tttatctact
    86641 ttgctatcat cagtaagtgg gaaaaggagg gtagaaaata atgaagaaga aatccagtat
    86701 ttatttagat attctctcac atgtactttt agttggtgcg accatcgttg cagttttccc
    86761 attggtatgg attatcatat cttctgtcaa agggaaaggg gaattaactc agtatccaac
    86821 acgattttgg cctgaacagt ttacattaga ttatttcact catgttatca acgatttgca
    86881 cttcattgat aacattcgaa acagtttaat cattgccttg gctacaaccc ttattgcgat
    86941 tattatttct gctatggcag cctatggtat tgttcgattc tttcctaaat tgggagcaat
    87001 catgtcgaga ctactcgtca ttacctacat tttcccacca attttgttag caattcccta
    87061 ttcaattgcc attgctaaag ttgggttaac aaatagttta tttggcttga tgatggttta
    87121 tctatctttt agtgttccat atgcagtttg gctcttagtt ggatttttcc aaacagttcc
    87181 aattggaatt gaagaagcgg ctagaattga tggtgcaaat aaatttgtta cgttttataa
    87241 agttgtgcta ccgattgtag caccaggtat tgtagcaaca gctatttata catttatcaa
    87301 tgcttggaat gaattcctgt atgccttgat tttgattaac aatacaggaa agatgacagt
    87361 agcagtagcc cttcgttcac ttaatggttc agaaatacta gactggggag atatgatggc
    87421 agcgtctgtt attgtagttc ttccatcaat tattttcttc tctatcatcc aaaataagat
    87481 tgcaagtgga ttatcagaag gatctgtgaa gtaatactca atgaaaatca aagagcaaac
    87541 taggaaacta gccgcaggct gctcaaagta cagctttgag gttgcagata aaactgacga
    87601 agtcagtaac catacgcaca gtaaggcgac gttgacgcgg tttgaagaga ttttcgaaga
    87661 gtataagtta aaattagaaa gtttattcaa taataaagga gaaaaaacat ggttaaatac
    87721 ggtgttgttg gagcagggta ttttggagct gaattggctc gctacatgca aaaaaatgat
    87781 ggagcagaga ttactcttct ctatgatcca gataatgcag aggcgattgc agaagaattg
    87841 ggagcaaaag tagcaagttc cttagatgag ttggtttcta gcgatgaagt agattgtgtt
    87901 atcgtcgcaa ctccaaataa tcttcataag gaaccggtta ttaaggctgc acagcatggt
    87961 aaaaatgttt tctgtgaaaa accaattgcg ctttcttatc aagattgtcg cgagatggta
    88021 gatgcgtgta aagaaaacaa tgtaaccttt atggcaggac atattatgaa tttctttaat
    88081 ggtgttcatc atgcaaaaga actcatcaat caaggagtta tcggagacgt tctatattgt
    88141 catacagctc gtaatggttg ggaagaacaa caaccgtcag tgtcatggaa aaaaattcgt
    88201 gaaaaatcag gtggtcactt gtatcaccac atccatgaat tggattgcgt tcaattcctt
    88261 atggggggca tgcctgaaac tgtaaccatg acaggtggaa atgtggccca tgaaggtgaa
    88321 catttcggtg atgaagatga tatgattttt gtcaatatgg aattttctaa taagcgtttt
    88381 gccttgttag aatggggttc agcttatcgt tggggtgaac attatgtctt aatccaagga
    88441 agcaaaggtg ccatccgctt agacttattc aactgtaaag gaactcttaa gctagatggg
    88501 caagaaagct atttcttgat tcacgaatcg caagaagaag atgatgatcg gactcgtatc
    88561 tatcatagta cagagatgga tggagcaatt gcttatggta aaccaggtaa acgtactcca
    88621 ttatggctat catctgtcat tgataaagaa atgcgctatc tgcatgagat tatggaagga
    88681 gccccagtat cagaagaatt tgcaaaactt ttgacaggtg aagctgccct agaagcaatt
    88741 gctactgcag atgcttgtac ccagtctatg tttgaagatc gcaaagtaaa attgtcagaa
    88801 attgtaaaat aaattttggt attctcctat ttataggtcg acttgctcct ctgaaagtac
    88861 ttttagagga gctgtttgac tttgctagtt tttgaaactg aaatctatta tactacaaac
    88921 tattgaaagc gttttaattt taaggtataa taatctcata gaaataaaga aaaggaggaa
    88981 agaggatgcc acagattagc aaagaagcct tgattgagca aatcaaagat ggaatcatcg
    89041 tttcttgtca ggctcttcct catgaaccgc tttatacaga agcgggaggg gtgattccct
    89101 tgctggtcaa agcggctgag caaggtggag cagtcggtat ccgagcaaac agtgttcgcg
    89161 atatcaagga aattaaggaa gtcactaaac ttccaatcat tgggattatc aaacgtgatt
    89221 atccacctca ggaacccttc atcacggcta ctatgaaaga agttgatgaa ttggcagaac
    89281 tggacatcga ggtgattgct ctggattgta ccaagcgtga acgctacgat ggtttggaaa
    89341 ttcaagagtt cattcgtcag gttaaggaga aatatcctaa tcagcttttg atggctgata
    89401 ctagtatctt cgaagaaggg ctagcagctg tagaagcagg aattgacttt gtcggaacaa
    89461 ccttatcagg ctacacatcc tacagtccaa aagtagacgg tccagatttt gaattgatta
    89521 agaaactctg tgatgccggt gtagatgtca ttgcagaagg aaaaattcat acaccagaac
    89581 aagccaaaca aatccttgaa tatggagtgc gaggcatcgt tgttggtggc gccattacta
    89641 gaccaaaaga gattacagaa cgcttcgttg ctagtcttaa ataagatgtg agagggagtt
    89701 ttatgtttaa agttttacaa aaagttggaa aagcttttat gttacctata gctatacttc
    89761 ctgcagcagg tctacttttg gggattggtg gtgcactttc aaacccaacc acgatagcaa
    89821 cttatccaat actagacaat agtatttttc aatcaatatt ccaagtaatg agctctgcag
    89881 gagaggttgt attcagtaat ttgtcactac ttctctgtgt gggattatgt attggcttag
    89941 cgaaacgaga taaaggaacc gctgcgttag caggagtaac tggttactta gttatgactg
    90001 caacgatcaa agctttggta aaacttttta tggcagaagg atctgcaatt gatactggag
    90061 ttattggagc attagttgtc ggaatagttg ccgtatattt gcacaaccga tataacaata
    90121 ttcaattacc ttccgcttta ggattctttg gaggttcacg cttcgttcct attgttacat
    90181 cgttctcttc tatcttgatt ggctttgtct tctttgttat ttggccacct ttccaacaac
    90241 ttcttgtttc tacaggtgga tatatttctc aggcgggtcc aattggaact tttctatatg
    90301 gatttttaat gagactttct ggagcagtag gcttacatca tataatttac cctatgtttt
    90361 ggtatactga acttggtggt gttgaaactg ttgcaggaca aacagtggtt ggagctcaaa
    90421 aaatattttt tgctcaatta gccgatttgg cccattctgg attatttaca gaaggaacaa
    90481 ggttttttgc aggtcgtttc tcaacaatga tgttcggttt accggctgcc tgtttagcga
    90541 tgtaccatag tgttcctaaa aatcgtcgta aaaaatacgc gggtttgttt tttggagttg
    90601 ctttaacatc ttttattacc ggtattacag aaccaattga atttatgttt ctattcgtca
    90661 gtccggttct atatgttgtt cacgcattcc ttgatggtgt tagcttcttt attgcagacg
    90721 tcttaaatat ttcaatagga aacacatttt caggaggtgt aatcgatttc actttatttg
    90781 gaattttgca ggggaacgct aagacgaatt gggttcttca gattccattt ggacttattt
    90841 ggagtgtttt gtattatatt atttttagat ggttcattac tcaattcaac gttctaacgc
    90901 caggacgagg agaagaagta gattctaaag aaatttctga atccgcagat tcaacttcaa
    90961 atactgcaga ttatttaaaa caggatagcc tacaaattat cagagccttg ggtggatcaa
    91021 ataatataga agatgtagat gcttgtgtga cacgtttacg tgtagctgta aaagaagtta
    91081 atcaagttga taaagcactt ttaaaacaaa ttggtgcagt tgatgtctta gaagtgaagg
    91141 gtggcattca agcaatatat ggagcaaaag caatcttata taaaaatagt attaatgaaa
    91201 ttttaggtgt agatgattaa gtacttactg acttaataaa aaacagagga gagtgatgga
    91261 tgagtaggat gaaatgaaat cgcatacaag aaataaagaa ctcattatcc aagttggata
    91321 cgcttattac ataggagaat acaaatgaaa tttagaaaat tagcttgtac agtacttgcg
    91381 ggtgctgcgg ttcttggtct tgctgcttgt ggcaattctg gcggaagtaa agatgctgcc
    91441 aaatcaggtg gtgacggtgc caaaacagaa atcacttggt gggcattccc agtatttacc
    91501 caagaaaaaa ctggtgacgg tgttggaact tatgaaaaat caatcatcga agcgtttgaa
    91561 aaagcaaacc cagatataaa agtgaaattg gaaaccatcg acttcaagtc aggtcctgaa
    91621 aaaatcacaa cagccatcga agcaggaaca gctccagacg tactctttga tgcaccagga
    91681 cgtatcatcc aatacggtaa aaacggtaaa ttggctgagt tgaacgacct cttcacagat
    91741 gaatttgtta aagatgtcaa caatgaaaac atcgtacaag caagtaaagc tggagacaag
    91801 gcttatatgt atccgattag ttctgcccca ttctacatgg caatgaacaa gaaaatgtta
    91861 gaagatgctg gagtagcaaa ccttgtaaaa gaaggttgga caactgatga ttttgaaaaa
    91921 gtattgaaag cacttaaaga caagggttac acaccaggtt cattgttcag ttctggtcaa
    91981 gggggagacc aaggaacacg tgcctttatc tctaaccttt atagcggttc tgtaacagat
    92041 gaaaaagtta gcaaatatac aactgatgat cctaaattcg tcaaaggtct tgaaaaagca
    92101 actagctgga ttaaagacaa tttgatcaat aatggttcac aatttgacgg tggggcagat
    92161 atccaaaact ttgccaacgg tcaaacatct tacacaatcc tttgggcacc agctcaaaat
    92221 ggtatccaag ctaaactttt agaagcaagt aaggtagaag tggtagaagt accattccca
    92281 tcagacgaag gtaagccagc tcttgaatac cttgtaaacg gatttgcagt attcaacaat
    92341 aaagacgaca agaaagtcgc tgcatctaag aaattcatcc agtttatcgc agatgacaaa
    92401 gagtggggac ctaaagacgt agttcgtaca ggtgctttcc cagtccgtac ttcatttgga
    92461 aaactttatg aagacaaacg catggaaaca atcagcggct ggactcaata ctactcacca
    92521 tactacaaca ctattgatgg ttttgctgaa atgagaacac tttggttccc aatgttgcaa
    92581 tctgtatcaa atggtgacga aaaaccagcg gatgctttga aagccttcac tgaaaaagca
    92641 aacgaaacaa tcaaaaaagc tatgaaacaa tagtccttag ttattctata aaaagtagtt
    92701 ttttaaagaa cctaagagtg tataccccct tttccctcta cacagatagt gtaagaaaag
    92761 ggggcttttg tttaaaatgt aagaaactgt cacgaaatta aaatgaagtt cttacataag
    92821 cgaatcataa aaaatttcat tttgatttta aaacagttca agaaagtcaa aaaattattc
    92881 tatttgaaag agaggtgccg actgtgaaag tcaataaaat ccgtatgcgg gaaacagtga
    92941 tttcctacgc tttcctagca ccagtattat tcttctttgt catctttgtg ttggctccga
    93001 tggtgatggg cttcattaca agtttcttta actactcaat gactaaattt gagtttgtag
    93061 gcttggataa ctatatccgt atgtttaaag atcctgtctt tacaaaatct ctgattaaca
    93121 cagttatttt ggttattgga tctgtaccag ttgttgttct attctcactc tttgtagcat
    93181 ctcagaccta tcatcaaaat gtcattgcca gatccttcta ccgtttcgtc ttcttccttc
    93241 ctgttgtaac gggtagtgtt gccgtgacag ttgtttggaa atggatttat gacccgctat
    93301 cagggattct aaactttgtc cttaagtcaa gccacatcat cagccaaaac atttcttggt
    93361 tgggagataa aaactgggca ttgatggcga ttatgattat tctcttgacc acttcagttg
    93421 gtcagcccat catcctttat atcgctgcca tggggaatat tgacaattca ctggttgaag
    93481 cggcgcgtgt tgatggtgca actgagtttc aagttttttg gaagattaaa tggccaagcc
    93541 ttcttccaac aactctttat attgcaatca tcacaacaat taactcattc cagtgtttcg
    93601 ccttgattca gcttttgaca tctggtggtc caaactactc aacaagtacc ttgatgtact
    93661 acctttacga aaaagccttc caattgacag aatacggcta tgccaacaca attggtgtct
    93721 tcttggcagt catgattgct atcgtaagct ttgttcaatt taaagtactt ggaaacgacg
    93781 tagaatacta aagaaaggag acagctatgc aatctacaga aaaaaaacca ttaacagcct
    93841 ttactgttat ttcaacaatc attttgctct tgttgactgt gctgttcatc tttccattct
    93901 actggatttt gacaggggca ttcaaatcac aacctgatac aattgttatt cctcctcagt
    93961 ggttccctaa aatgccaacc atggaaaact tccaacaact catggtgcag aaccctgcct
    94021 tgcaatggat gtggaactca gtatttatct cattggtaac catgttctta gtttgtgcaa
    94081 cctcatctct agcaggttat gtattggcta aaaaacgttt ctatggtcaa cgcattctat
    94141 ttgctatctt tatcgctgct atggcgcttc caaaacaagt tgtccttgta ccattggtac
    94201 gtatcgtcaa cttcatggga atccacgata ctctctgggc agttatcttg cctttgattg
    94261 gatggccatt cggtgtcttc ctcatgaaac aatttagtga aaatattcct acagagttgc
    94321 ttgaatcagc taaaatcgac ggttgtggtg agattcgtac cttctggagt gtagccttcc
    94381 cgattgtgaa accagggttt gcagcccttg caatctttac cttcatcaat acttggaatg
    94441 actacttcat gcaattggta atgttgactt cacgtaacaa tttgaccatc tcacttgggg
    94501 ttgcgaccat gcaggctgaa atggcaacca actatggttt gattatggca ggagctgccc
    94561 ttgctgctgt tccaatcgtc acagtcttcc ttgtcttcca aaaatccttc acacagggta
    94621 ttactatggg agcggtcaaa ggataatact ctgcgaaaat ctcttcaaac tacgtcagct
    94681 tcaccttgcc atacttaagt attgcctgcg gttagcttcc tagtttgttc ttcaattttc
    94741 attgagtata ggaaaatcaa tctatcaaga tacagaagta tattttatag atttagagaa
    94801 tatagaggtt ataagtgtct acaaaatgga gggtatgcag ttactttatg aagttttgtc
    94861 agacacttat caacttaaga atggttttag ttaactatca gaaacgaagg aaagagtatg
    94921 atttttgacg atttgaaaaa catcaccttt tacaaaggga tttatcctaa tttagacaag
    94981 gctatcgact atctctacca acatcgtaag gattctttcg aattaggaaa gtatgatatt
    95041 gatggagata aagtctttct agttgttcag gaaaatgtcc tcaatcaagc tgaaaatgat
    95101 caatttgagt atcataagaa ctatgcagat ttgcatttgc tgatagaagg gcatgaatat
    95161 tcgagctacg gttcacgtat caaagacgag gcagtagcat tcgacgaagc gagtgacatt
    95221 ggctttgttc attgttatga acactaccca ctcttattgg gttatcacaa ttttgcgatt
    95281 ttcttcccag gtgagccaca tcagccaaat ggttatgcag gcatggaaga aaaggttcga
    95341 aaatatctct ttaaaatctt gattgattaa aaataggatg aattgttttt ttgtaaagct
    95401 ttgataatac tctaccatga aattgatctt tgtgaggtag agaaatgaga ataaaatatt
    95461 taaaaattgg tatcttctaa gtatgctgca agagctagtt tcttggatgg acagggaatt
    95521 acagttgatg agatggcttg gataattagg ggcattgtga atgcattgat tggtagatac
    95581 ataaaattag gtgcttatgc ggctaagtat ggtattagta tggcacgctc gatcttaagt
    95641 agggcagctg caactgcggc agcaagagta ggattattga ccaagatttt tggatggatt
    95701 ttacgaatag ctgtgctcgc agctgatgta tatggtaatt ttgccaacaa tattgctgca
    95761 gcttgggatg catatgataa aattcctaac aatggtcgta taaactttta aaatgcgaga
    95821 atgaaagcac tttgtatttt tttattgaat atgttagctt ggacagtgct tgcaatgata
    95881 attcgtggag ggctagatgg atttaatgag catacttgga gtactatttt aattgcttcg
    95941 ctgtccggga tatatataga ttatatgtcc atagataaaa gtagaaaaaa gtccaaaaga
    96001 aaaaagaaaa aatagatttg ttcatggtag ggacttatga aagttttact gacaaaaaag
    96061 aaaacagttt acaaagaaaa atgatggagg agcaaacatg gcacaaaaag gagtaagcct
    96121 tatcaaggca gcatttgata cagataactt tctcatgcgt tttagtgaga aagtcttgga
    96181 catcgtgaca gccaatcttc tttttgtcgt ctcttgtttg cccatcgtga cgattggagt
    96241 ggctaaaatc agcctctacg agaccatgtt cgaagttaag aagagcagac gggtgcctgt
    96301 ttttaaaatc tatctaagat ctttcaagca aaatctgaaa ctaggtcttc agctgggttt
    96361 aatggagtta ggaattgtgt ttcttaccct ttcagatctc tatcttttct ggggtcaaac
    96421 agctctgccc ttccaattgc tgaaagccat ttgtttaggt attctgattt ttcttactat
    96481 cgtgatgctg gctagttacc ctatcgcggc acgttatgac ctatcttgga aagaaattct
    96541 tcaaaaagga ttgatgttgg ctagttttaa ctttccttgg ttcttcctca tgttagccat
    96601 tcttgtcctc attgtgatgg ttctttatct gtccgccttc agtctactct taggtggctc
    96661 agtcttccta ctttttgggt ttggactatt ggtctttatc cagactggat tgatggagaa
    96721 aattttcgca aaataccaat aggagcttta tttctgaaac tactttcaaa ggctccaaac
    96781 gctattctat aagcgagaaa ctaaaatcta agtgtctata agatattgaa agccttttcg
    96841 gtaaagtgta tactaaagaa gtaaagaaaa atctgcttta gcagaaaata aaaataatag
    96901 gagaaaatct atgtcagatt tgaaaaaata cgaaggtgtc attccagcct tctacgcatg
    96961 ttatgatgat caaggagaag taagcccaga acgtacgcgt gccttggttc aatacttcat
    97021 tgataaaggt gttcaaggtc tttatgtcaa tggttcttct ggtgaatgta tctaccaaag
    97081 cgttgaagat cgcaagttga ttttggaaga agtcatggcg gtagccaaag gtaaattgac
    97141 cattattgcc catgttgctt gcaataatac taaagatagt atggaacttg ctcgccatgc
    97201 tgaaagcttg ggagtagatg ctattgcaac gattccacca atttacttcc gcttgccaga
    97261 atactcagtt gctaaatact ggaacgatat tagttctgca gctccaaata cagactacgt
    97321 gatttataac attcctcaat tggcaggggt tgctttgact ccaagccttt acactgaaat
    97381 gttgaaaaat cctcgtgtta tcggtgtgaa gaactcttct atgccagttc aagatatcca
    97441 aacctttgtg agccttggtg gggaagacca tatcgtcttt aacggtccag atgaacagtt
    97501 cctaggtggc cgcctcatgg gtgccaaagc tggtatcggt ggtacttatg gtgctatgcc
    97561 agaactcttc ttgaaactca atcagttgat tgcggataag gacctagaaa cagcgcgtga
    97621 attgcagtat gctatcaacg ctatcattgg taaattgact gctacacatg gaaatatgta
    97681 cggtgtcatc aaagaagttt tgaagatcaa tgaaggcttg aatattggtt ctgttcgttc
    97741 accattgaca ccagtgactg aagaagatcg tccagttgta gaagcggctg ctgccttgat
    97801 tcgtgaaacc aaggagcgct tcctctaatc taaaaggagg tatttatgac atactacgtt
    97861 gcaattgata tcggtggaac caacatcaag tatggtttgg ttgatcaaga ggggcaactt
    97921 cttgaatcgc atgaaatgcc aactgaggcg cataagggtg gacctcatat cttacaaaag
    97981 accaaagata tcgtagctag ttatttagaa aaaggcccag tagcaggtgt tgccatatct
    98041 tctgctggga tggtggatcc ggataagggt gagattttct atgctgggcc gcaaatccct
    98101 aactacgcag gcacccagtt caaaaaggaa atcgaagaaa gctttactat tccttgtgag
    98161 attgaaaatg atgtcaactg tgcaggtctt gctgaggcag tatctggttc aggcaaggga
    98221 gcaagtgtga cactttgctt gaccattgga acaggtatcg gtggttgctt gattatggat
    98281 aggaaagtct tccatggttt tagcaattca gcctgtgaag tcgggtatat gcatatgcag
    98341 gatggagctt ttcaagactt ggcttctaca acagctttag tgaaatatgt agctgaagcc
    98401 catggagaag atgttgatca gtggaatggc cgtagaattt tcaaagaagc cactgaagga
    98461 aacaaaatct gcatggaagg tattgaccgt atggttgact atctaggaaa aggtctggca
    98521 aatatttgct acgttgccaa tccagaagtg gttattcttg gtggtggtat catggggcaa
    98581 gaggctatcc tcaaacctaa gatccgcaca gccttgaaag aggctttggt accaagttta
    98641 gcagaaaaaa cacgattaga atttgcccat caccaaaata cagcagggat gttgggtgca
    98701 tattatcatt ttaagacaaa acaatcctag tttggctcag ccaaactagg attttcttac
    98761 acgtttttgt ctacgatagc cgttgagttt tttattttcc cagtagctat taaagatttt
    98821 ttccttgctt tcgcgattga tttccaaaaa gtaggcataa atcaaatcga taaagaagag
    98881 cataggaagt tgagcggata ttcgttggat ataggaggtt tggctatggg tggctacaag
    98941 aacagtctct gtataggtct ggctatcttt attgggaaca cttgtaaaga gtacagtctt
    99001 tgcccccatc tccttggcat ctaatagact atctaaaata gaaggagttg agcctgaaag
    99061 tgagaaaccc agtactagac aattttcatc catgatgctg gttgtccagg caaagccgtc
    99121 ttggtctgtc aaagcttcgc agaccacacc tagtcgcata aaacgtaatt tcatttcacg
    99181 ggcgacgagg ccagaactcc ctgttccaaa gaagtagata cgctcagcat cttcgattag
    99241 ctgggcaatt cgctctagtt ggatttcgtc aatcaagtct tgtgtttgtt ccctcatatt
    99301 gctataactt ctgaggactc gtttggtcag tggactatgc ttggagactt ggttggcttg
    99361 attttctgcc tgatgttggt attggaaaat aaattctcgg tagccagtaa agccacactt
    99421 tttagcaaag cgggtcaaag cagcttgaga aatatgtaat ttttgggtga cttgttgaga
    99481 agataaatca tctgtaatcg tttcagcttg caaaaaatag cgagcgattt cttgctctag
    99541 gtctgtcatt tcttcaaaat gtgaatcaat gatagttgcg atatctggtt tgtccatagg
    99601 gaaagctcct ttacatgagt catactggaa gactagatca gggaatagtc acacttcatt
    99661 ataacacata atataaggat agataaataa aaacacatct ctgttttaaa aacgaaaaaa
    99721 tcgaaaaagc ttctttcttt tccataattt tctactcaaa ttgtggtaca attaagagta
    99781 agattttaag ttagaaatga gactgatttg tatgagaaaa tttaacagcc attcgattcc
    99841 gattcggctt aatttattgt tttcaatcgt cattttactc tttatgacca ttattggtcg
    99901 tttgttgtat atgcaggttt tgaacaagga tttttacgaa aaaaagctag cttcagctag
    99961 tcagaccaag attacaagca gttcagcccg tggggaaatt tatgatgcta gtggaaaacc
   100021 tttggtagaa aatacgttaa agcaggttgt ttcctttacg cgtagcaata aaatgacggc
   100081 tacagactta aaagaaacag ctaaaaagtt actgacttat gtgagcatca gttctccaaa
   100141 tttgacagaa cgccagctgg cggattacta tttggctgat cctgaaatct ataaaaaaac
   100201 agtggaagct ctcccaagcg agaaacgctt gggttcagat ggcaatcgtc tatccgaatc
   100261 agaactgtat aacaatgcgg tcgatagtgt ccaaacaagt caactaaact atacagagga
   100321 tgaaaagaaa gaaatctatc tttttagtca gttaaatgct gttggaaact ttgcgacagg
   100381 aaccattgcg acagatcctc taaatgattc tcaggtggct gttattgcct ctatttcaaa
   100441 ggagatgcct ggcattagta tttctacttc ttgggataga aaggttttgg aaacttccct
   100501 ttcttctata gttgggagtg tatccagtga aaaagctggt ctcccagcgg aagaagcaga
   100561 agcctatctt aaaaaaggct attctctaaa tgatcgtgta ggaacctcct atttggaaaa
   100621 gcaatatgaa gagaccttac aaggaaaacg ctcggtaaaa gaaatccatc tggataaata
   100681 tggcaatatg gaaagcgtgg atacaattga ggaaggtagt aagggaaaca atatcaaact
   100741 gaccattgat ttggcattcc aagatagcgt ggatgcttta ctgaaaagtt atttcaattc
   100801 cgagctagaa aatggtggag ccaagtattc tgaaggtgtc tatgcagtcg cccttaaccc
   100861 aaaaacaggt gcggttttgt ctatgtcagg gattaaacat gacttgaaaa cgggagagtt
   100921 gacgcctgat tccttgggaa cggtaaccaa tgtctttgtt ccaggttcgg ttgtcaaggc
   100981 ggcgaccatc agctcaggtt gggaaaatgg agtcttgtca ggaaaccaga ccttgacaga
   101041 ccagtccatt gtcttccaag gttcagctcc catcaattct tggtatactc aggcttacgg
   101101 ttcattccct atcacagcgg tccaagctct ggagtattca tctaatacct atatggtcca
   101161 aacagcctta ggtcttatgg ggcaaaccta tcaacccaat atgtttgtcg gcaccagcaa
   101221 tctagagtct gctatggaga aactgcgttc aacctttggc gaatatggct tgggtactgc
   101281 gacaggaatt gacctaccag atgaatctac tggatttgtt cccaaagagt atagctttgc
   101341 taattacatt actaatgcct ttgggcagtt tgataactat acgccgatgc agttggctca
   101401 gtatgtagca actattgcaa ataatggtgt tcgtgtggct cctcgtattg ttgaaggcat
   101461 ttatggtaat aatgataagg gaggactggg tgacttgatt cagcaactgc aaccgacaga
   101521 gataaataag gtcaatatat ccgactccga tatgagcatc ttgcaccaag gtttttatca
   101581 ggttgcccat ggtactagtg gattgacaac tggacgtgcc ttttcaaatg gtgccttggt
   101641 atccattagc ggaaaaacag gtacagccga aagctatgtg gcagatggtc agcaagcaac
   101701 caataccaat gcggtggcct atgccccatc tgataatccc caaatcgctg tcgcagtggt
   101761 ctttcctcat aataccaatc taacaaatgg tgtaggacct tccattgcgc gtgacattat
   101821 caatctgtat caaaaatacc atccaatgaa ctagaaagga aattatgctt tatccaacac
   101881 ctattgccaa gttgattgac agttattcta agttaccagg tatcgggatt aagacggcta
   101941 cgcgtctggc cttttatacg attgggatgt ctgctgatga tgtcaatgaa tttgcaaaaa
   102001 atctcctttc tgctaagaga gaattgacat attgttctat ttgtggacgt ttgacagacg
   102061 acgatccttg ttctatctgt actgattcga ctcgtgacca gacaacaatt ttagttcttg
   102121 aggatagtag agatgtggca gccatggaaa atatccaaga ataccatgga ctctatcatg
   102181 tcctgcatgg cctcatttct cctatgaatg gtatcagtcc ggacgatatc aatcttaaga
   102241 gccttatgac tcgtcttatg gatagtgagg tttcagaagt gattgtggcg actaatgcta
   102301 cagcggatgg tgaagcgacc tccatgtatc tttcacgttt gctcaagccg gctggtatca
   102361 aggttacgcg tctagcacga ggtctcgctg tgggagcgga cattgagtat gcggacgaag
   102421 tgacactctt acgagcgatt gaaaatcgga cagagttgta agtgtaggca aatttacgaa
   102481 ctccattcat ttataaaaaa tcaaagaggc tgaaaatcgt tcctatcggc ctctttttgt
   102541 atagtgtgat gagtagaatc aggttcaagt tttaaaaaac caagcaaata tgatatacta
   102601 aagagcgagt attctagtag aattaggaca aataatatga aacaaacgat tattctttta
   102661 tatggtggac ggagtgcgga acgcgaagtc tctgtccttt cagctgagag tgtcatgcgt
   102721 gcggtcaatt acgaccgttt cacagtcaag actttcttta tcagtcagtc aggtgactct
   102781 atcaaaacgc aggaatttag tcatgctccg gggcaagaag accgtctcat gaccaatgaa
   102841 accattgatt gggataagaa agttgcacca agtgctatct acgaagaagg tgcagtggtc
   102901 tttccagtcc ttcacgggcc aatgggagaa gatggctctg ttcaaggatt cttggaagtt
   102961 ttgaaaatgc cttacgttgg ttgcaacatt ttgtcatcaa gtcttgccat ggataaaatc
   103021 acgactaagc gtgttctgga atctgctggt attgcccaag ttccttatgt ggctatcgtt
   103081 gaaggcgatg atgtgactgc taaaatagct gaagtggaag aaaaattggc ttatccagtc
   103141 ttcactaagc cgtcaaacat ggggtctagt gtcggtattt ctaagtctga aaaccaagaa
   103201 gaactccgtc aagccttaaa acttgccttc cgatatgaca gccgtgtctt ggttgagcaa
   103261 ggagtgaatg cccgtgaaat tgaggttggc ctcttgggta actacgatgt caagagcacg
   103321 ctacctggag aagttgtcaa ggacgttgcc ttttatgact acgatgccaa gtatattgat
   103381 aacaaggtta ctatggatat tcctgccaaa atcagtgatg atgtggtggc tgtcatgcgt
   103441 caaaatgcag aaacagcctt ccgtgccatt ggtggccttg gtctatctcg ttgcgatttc
   103501 ttctatacag ataagggaga gatttttctc aacgagctca ataccatgcc aggtttcacc
   103561 cagtggtcta tgtacccact actttgggaa aatatgggga tcagctaccc agaactaatc
   103621 gagcgtttgg ttgaccttgc caaggaaagt tttgacaagc gcgaagcgca tttgatataa
   103681 aatgaaagag agggtagaag ccagaaccat cactgcaagg tgactagagt tctcggactt
   103741 cagccctttt taaaggagta gaaatgaaat taacaatcca tgaaattgcc caagttgttg
   103801 gagccaaaaa tgatatcagt atctttgagg acactcagtt agaaaaagct gagtttgata
   103861 gtcgtttgat tggaactgga gatttatttg tgccacttaa aggtgcgcgt gatggccatg
   103921 actttattga aacagccttt gaaaatggtg cagcagtaac cttgtctgag aaagaggtct
   103981 caaatcatcc ttacatttta gtagatgatg ttttgacagc ctttcaatcc ctagcatcct
   104041 actatcttga aaaaacgact gttgatgtct ttgctgttac aggttcaaat ggcaagacaa
   104101 cgactaagga tatgttggcg catttactgt caacaagata caagacctac aaaacacaag
   104161 gcaattacaa taatgagatt ggccttcctt acacagttct tcatatgcct gaaggaacag
   104221 aaaagttggt tttggagatg ggacaggatc acttgggaga tattcatctc ttgtctgaat
   104281 tggctcgtcc aaaaacagcc atcgtgacct tggttggaga agctcatttg gcctttttca
   104341 aagatcgttc tgagattgct aaaggaaaaa tgcaaattgc agacggaatg gcttcaggtt
   104401 ccttgctttt agcgccggct gaccctatcg tagaggacta tttgccaatt gataaaaagg
   104461 tggttcgttt tggtcaagga gcagagctgg aaattactga cttggttgag cgtaaggaca
   104521 gtctgacctt taaggctaat ttcttggaac aagcccttga tttgccagta actggcaagt
   104581 acaatgcgac aaatgctatg attgcatcct atgttgcctt gcaagaagga gtttcagagg
   104641 agcaaattcg tttggcattc caacatcttg aattgacgcg taaccgtacc gagtggaaga
   104701 aagcagccaa tggagcagat atcctgtcag atgtttacaa tgccaatcca actgctatga
   104761 aactgatttt agagactttc tctgccattc cagccaatga aggtggcaag aaaattgcag
   104821 tgttggcgga tatgaaggag cttggtgacc agtctgttca acttcataat cagatgattt
   104881 tgagcctctc cccagatgtg cttgatatcg ttattttcta tggagaagat attgctcagt
   104941 tggctcaatt ggccagtcaa atgtttccaa tcggccacgt ttactacttc aagaaaacag
   105001 aagaccagga tcaatttgaa gacctagtca agcaggttaa ggaaagcctt ggagcctatg
   105061 accaaatctt gctaaaaggt tctaactcta tgaatctagc caaattggta gaaagtttag
   105121 aaaatgaatg caagtgattt tgctaagtat cttcaaagaa tgattgctct tacagatact
   105181 ggactaacct ttacaaagga tccgtttgac cgtgagcgct acgaagactt gcgaagtctg
   105241 ttatctgaaa tgttgaatca ggtatcagac ctggatgcag acgaagtggc agaactcttg
   105301 aaaccgacct ccgcttatgc gactccttta atggacgtcc gtgcttggat agttgaggat
   105361 gaaaaaattt gtctggttag gggacaagga gagaatgatt gggctttgcc aggtggcttt
   105421 ggcgaggtcg gttattcacc aactgaaaat attctcaagg aaattgaaga agaaaccggt
   105481 tttaaagcca aagttgaacg actactagct gtttttgata ccaatcgttt ccaactacag
   105541 agcaaacaat atgcaaagtt tgtttttgaa tgtaagcttc tcgatggaca atttcaagaa
   105601 aatcaagaaa tcgctgacct tcaatttttt gcaattgacc aactgccgaa tttatctgaa
   105661 aaacgcatta ccaaggagca aatagagctt ctttggcagg tttatcaagg tcataggggg
   105721 caatatcttg actaagaaga tgattatcgt atttctaaat ccatttttaa caactagcat
   105781 ggtataataa tatgcaggaa aattttgaat tatgaggaag actagatgaa tttatgggat
   105841 attttcttta cgactcaggc aaccgagccg cccaaatttg accttttttg gtatgttagc
   105901 ctatttacgc tcttagcctt aaccttttat acagcccatc gctatcgtga aaataaggtt
   105961 taccaacgat ttttccaaat cttgcagact gttcagttaa tccttcttta tggttggtac
   106021 tgggtcaatc atatgccact gtcagaaagc ctaccctttt accattgccg tatggctatg
   106081 tttgtggtac tcttgcttcc tggtcaatcc aaatataaac aatactttgc attattggga
   106141 acatttggga cattagcagc ctttgtttat ccagtgccag atgcttaccc ttttccacat
   106201 atcaccattc tatcctttat ctttggtcat ttagcactct tggggaactc tctagtttat
   106261 ctattgagac agtataatgc gcgattgctg gatgtgaagg gaatttttct catgaccttt
   106321 gccctaaatg ccttgatttt tgtggtcaat ttggtgacag gtggcgatta cggatttttg
   106381 acaaaaccgc cattggttgg ggatcacggt ctagtagcta attatttact tgtttcaatt
   106441 gtgctggtag ctactatcag tttgactaag aaaatcttag aattcttttt agctcaagaa
   106501 gcagaaaaaa tgattgcaaa ggaagcttaa cacagagctt tcttttttgc tcttagagag
   106561 tttttacaag cagcttataa aataagaatt tctgaataga caaactcaaa aaatggctgg
   106621 gaaatttagg aaaaaagcaa gcacgattaa attttttgtg ttataatatt ttgtgaatag
   106681 ctatgcctat gtttagctat ggaataatac gaagtgcgaa acttggaaga tagagaggaa
   106741 gcgatgtaat ggctagagaa ggctttttta caggtctaga tattggaaca agctctgtca
   106801 aggtgcttgt ggccgagcag agaaatggtg aattaaatgt aattggcgtg agtaatgcca
   106861 aaagtaaagg tgtaaaggat ggaattattg ttgatattga tgcagcagca actgctatca
   106921 agtcagccat ttcccaagcg gaagaaaagg caggcatttc gattaaatca gtgaatgtcg
   106981 gcttgcctgg taatcttttg caggtagaac caactcaggg gatgattcca gtaacatctg
   107041 atactaagga aattacggat caagatgttg aaaatgttgt caaatcagct ttgacaaaga
   107101 gtatgacacc tgaccgtgaa gtcattacct ttattcctga agaatttatt gtggatggtt
   107161 tccaagggat tcgtgaccca cgtggcatga tgggggttcg ccttgaaatg cgtggtttgc
   107221 tttatacagg acctcgtact atcttgcaca atttgcgtaa gacggttgag cgtgcaggtg
   107281 ttcaggttga aaatgttatc atttcaccac tagcaatggt tcagtctgtt ttgaacgaag
   107341 gggaacgtga atttggtgct acagtgattg atatgggggc aggtcaaacg actgtcgcta
   107401 caatccgtaa tcaagaactc cagttcacac atattctcca agaaggtgga gattatgtaa
   107461 ctaaagatat ctccaaggtt ttgaaaacct ctcgcaaatt agcggaaggc ttgaaactga
   107521 attacgggga agcctatccg cctcttgcaa gcaaagaagc cttccaagta gaggttattg
   107581 gagaagtaga agcagtcgaa gtgacggaag cctacttgtc agaaattatt tctgcacgaa
   107641 tcaagcacat ccttgaacaa atcaagcaag aattagatag aaggcgtcta ttggacctcc
   107701 ctggtggtat tgtcttaatc ggtgggaatg ccattttacc aggtatggtt gagcttgctc
   107761 aggaagtctt tggcgtccgt gtcaagcttt atgttccaaa tcaagttggt atccgtaatc
   107821 cagcctttgc gcatgtgatt agtttatcag aatttgcggg tcaattaaca gaagttaatc
   107881 ttttggctca gggagcgata aaaggtgaga atgacttaag tcatcagcca attagttttg
   107941 gtgggatgct gcaaaaaaca gctcagtttg tacaatcaac gcctgttcaa ccagctcctg
   108001 ctccagaagt agagctggtg gcgcctacag aaccaatggc ggatttccaa caagcttcac
   108061 aaaataaacc gaaattagca gatcgtttcc gtggcttgat cggaagcatg tttgacgaat
   108121 aaagaggaaa aataaattat gacattttca tttgatacag ctgctgctca aggggcagtg
   108181 attaaagtaa ttggtgtcgg tggaggtggt ggcaatgcca tcaaccgtat ggtcgacgaa
   108241 ggtgttacag gcgtagaatt tatcgcagca aacacagatg tacaagcatt gagtagtaca
   108301 aaagctgaga ctgttattca gttgggacct aaattgactc gtggtttggg tgcaggaggt
   108361 caacctgagg ttggtcgtaa agccgctgaa gaaagcgaag aaacactgac ggaagctatt
   108421 agtggtgccg atatggtctt catcactgct ggtatgggag gaggctctgg aactggagct
   108481 gctcctgtta ttgctcgtat cgccaaagat ttaggtgcgc ttacagttgg tgttgtaaca
   108541 cgtccctttg gttttgaagg aagtaagcgt ggacaatttg ctgtagaagg aatcaatcaa
   108601 cttcgtgagc atgtagacac tctattgatt atctcaaaca acaatttgct tgaaattgtt
   108661 gataagaaga caccgctttt ggaggctctt agcgaagcgg ataacgttct tcgtcaaggt
   108721 gttcaaggga ttaccgattt gattaccaat ccaggattga ttaaccttga ctttgccgat
   108781 gtgaaaacgg taatggcaaa caaagggaat gctcttatgg gtattggtat cggtagtgga
   108841 gaagaacgtg tggtagaagc ggcacgtaag gcaatctatt caccacttct tgaaacaact
   108901 attgacggtg ctgaggatgt tatcgtcaac gttactggtg gtcttgactt aaccttgatt
   108961 gaggcagaag aggcttcaca aattgtgaac caggcagcag gtcaaggagt gaacatctgg
   109021 ctcggtactt caattgatga aagtatgcgt gatgaaattc gtgtaacagt tgtcgcaacg
   109081 ggtgttcgtc aagaccgcgt agaaaaggtt gtggctccac aagctagatc tgctactaac
   109141 taccgtgaga cagtgaaacc agctcattca catggctttg atcgtcattt tgatatggca
   109201 gaaacagctg aattgccaaa acaaaatcca cgtcgtttgg aaccaactca ggcatctgct
   109261 tttggtgatt gggatcttcg ccgtgaatcg attgttcgta caacagattc agtcgtttct
   109321 ccagtcgagc gctttgaagc cccaatttca caagatgaag atgaattgga tacacctcca
   109381 tttttcaaaa atcgttaagt aaatgaatgt aaaagaaaat acagaacttg tttttcgaga
   109441 agttgcagag gctagtctga gtgctaatcg agagcgtggt tcggtctctg tcattgcagt
   109501 taccaagtat gtagatgtac cgacagcgga agccttgctt ccgctaggtg ttcatcatat
   109561 cggtgaaaat cgtgtagata agtttctgga aaaatatgaa gctttaaaag atcgagatgt
   109621 gacttggcat ttgattggta ccttgcaaag acgtaaggtg aaagatgtca ttcaatacgt
   109681 tgattatttc catgcattgg actcagtaaa gctagcaggg gaaattcaaa aaagaagtga
   109741 ccgagtcatc aagtgtttcc ttcaagtaaa tatttctaaa gaagaaagca aacacggttt
   109801 ttcgagagag gaactgctgg aaatcttgcc agagttagcc agactagata agattgaata
   109861 tgttggttta atgacgatgg caccttttga ggctagcagt gagcagttga aagagatttt
   109921 caaggcgacc caagatttac aaagagaaat tcaagagaaa caaattccaa atatgcctat
   109981 gaccgagtta agtatgggaa tgagtcgtga ttataaagaa gcgattcaat tcggttccac
   110041 ttttgttcgt ataggtacat cattttttaa gtaggagaga accatgtctt taaaagatag
   110101 attcgataga tttatagatt attttacgga ggatgaggat tcaagtctcc cttatgaaaa
   110161 aagagatgag cctgtgttta ctccagtaaa ttcttcacag gaaccggctc tcccaatgaa
   110221 tcaaccttca cagtcggctg gtacaaaaga gaacaatatc accagacttc atgcaagaca
   110281 acaggaattg gcaaatcaga gtcagcgtgc aacggataag gtcattatag atgttcgtta
   110341 tcctagaaaa tatgaggatg caacagaaat tgttgattta ttggcaggaa acgaaagtat
   110401 cttgattgac tttcagtata tgacagaggt gcaggctcgt cgttgtttgg actatttgga
   110461 tggagcttgt catgttttag ctggaaattt gaaaaaggta gcttctacca tgtatttgtt
   110521 gacaccagtg aacgttattg taaatgttga agatatccgt ttaccagatg aagatcaaca
   110581 gggtgagttc ggttttgata tgaagcgaaa tagagtacga taatgatttt tttaattcgt
   110641 atgatttata atgcagtgga tatttactcc ctgattttgg tagccttcgc tgtcatgtct
   110701 tggtttccag gtgcctacga atccagttta ggtcgttgga ttgtagcgtt ggtgaaacca
   110761 gtgcttgctc ccttgcaacg cctgccttta cagatagcgg gtcttgattt atctgtttgg
   110821 gttgcgattg ttttggttcg atttttagga gaaaacctag tgcgttttct ggcgatgata
   110881 ggatgaataa agggatttat cagcatttct ccatagaaga tcgtccattt cttgacaagg
   110941 gaatggaatg gataaagaag gtagaagata gctatgctcc ttttttaact ccttttatca
   111001 atcctcatca ggagaagcta ttaaagattt tggccaaaac ctatggtctt gcttgtagca
   111061 gtagtgggga attcgtctcg agtgagtatg ttcgagtttt attataccca gattatttcc
   111121 aaccagagtt ttcagatttt gaaatatctc tccaggaaat tgtgtattcc aataaatttg
   111181 aacatttaac gcatgctaag attttaggga cagtcatcaa tcaattaggg attgaacgga
   111241 aactttttgg agatatccta gtagatgaag aacgggcgca gattatgatt aatcagcagt
   111301 ttcttcttct ctttcaagat ggactaaaga aaattggtcg tatacctgtt tcgctggagg
   111361 aacgtccttt caccgagaaa atagataagc tagaacagta tcgagaactg gatttatctg
   111421 tgtctagttt tcgattagat gttcttttat caaatgtttt gaaactatct aggaatcaag
   111481 caaaccagtt gattgaaaag aaacttgtcc aagtaaatta tcatgtggta gacaaatcag
   111541 attacactgt tcaagttgga gacttgatta gtgtgagaaa atttggtcgc ttgagattac
   111601 ttcaagataa gggacaaacg aaaaaagaga agaaaaaaat aaccgtccag ttattattaa
   111661 gtaagtgagg aatagaatgc caattacatc attagaaata aaggacaaga cttttggaac
   111721 tcgattcaga ggttttgatc cagaagaagt cgatgaattt ttagatattg tggttcgtga
   111781 ttacgaagat cttgtgcgtg cgaatcatga taaaaatttg cgtattaaga gtttagaaga
   111841 gcgtttgtct tactttgatg aaataaaaga ttcattgagc cagtctgtat tgattgccca
   111901 ggatacagct gagcgagtga aacaggcggc gcatgaacgt tcaaacaata tcattcatca
   111961 agcagagcaa gatgcgcaac gcttgttgga agaagctaaa tataaggcaa acgagattct
   112021 tcgtcaagca actgataatg ctaagaaagt cgctgttgaa acagaagaat tgaagaacaa
   112081 gagccgtgtc ttccaccaac gtctcaaatc tacaattgag agtcagttgg ctattgttga
   112141 atcttcagat tgggaagata ttctccgtcc aacagctact tatcttcaaa ccagtgatga
   112201 agcctttaaa gaagtggtta gcgaagtact tggagaaccg attccagctc caattgaaga
   112261 agaaccaatt gatatgacac gtcagttctc tcaagcagaa atggaagaat tacaagctcg
   112321 tattgaggta gccgataaag aattgtctga atttgaagct cagattaaac aggaagtgga
   112381 aactccaact cctgtagtga gtcctcaagt tgaagaagag cctctgctcg tccagttggc
   112441 ccaatgtatg aagaaccaga agtagctcca gtgcatccga caggtccaac accagctaca
   112501 gaaactgttg attcagcacc gggatttgaa gcaccgcaag aatctgttac aattttataa
   112561 gaaatattct gagaacaata tcttatcctt atattcccag cgagcaggag atggtgtgag
   112621 tcctgcattc cctatcgata agattatcct ctcaaactat caagtctgaa tctagtaaga
   112681 tttgacgttc cccacgttac gggataagag ggagaaagac taaaactttt tccgaataaa
   112741 ggtggtacca cgattttcgt cctttttgga agtcgtggtt tttaatttgt tattatttat
   112801 aaaggagata ccatgaaact aaaagatacc cttaatcttg ggaaaacagc tttcccaatg
   112861 cgtgcaggcc ttccgaccaa agagccagtt tggcaaaagg aatgggaaga tgcaaaactt
   112921 taccaacgtc gtcaagaact aaatgaaggg aaaccgcatt ttgtcttgca tgatggccct
   112981 ccatacgcaa atggaaatat ccacgttgga catgccatga atcatatctc aaaagatatc
   113041 atcattcgtt ctaagtccat gtcaggtttt aactcacctt atatcccagg ttgggatact
   113101 catggtctgc caatcgagca agttttggca aagcaaggtg ttaagcgcaa agaaatggat
   113161 ttggttgagt acttgaaact ttgccgtgaa tacgctcttt ctcaagttta taaacaacgt
   113221 gatgatttca agcgcttggg gatgtcaggt gactgggaaa atttatatgt gactttaact
   113281 ccagactacg aagcagccca aatccgtgta tttggtgaga tggctaataa gggttatatc
   113341 taccgtggtg ctaagccagt ttactggtct tggtcatctg agtcagccct tgccgaagcg
   113401 gaaattgaat accatgattt ggtttcaact tccctttact atgccaataa ggtaaaagat
   113461 ggcaaaggtg ttctagatac agatacttat atcgttgttt ggacaactac tccatttact
   113521 atcacagctt ctcgtggttt gactgttggt gcagatattg attacgtttt ggttcaacct
   113581 gctggtgaaa ctcgtaagtt tgtcgttgct gcagaattgt tgactagctt gtctgagaaa
   113641 tttggctggg ttgatgttca agttttggca acttaccgtg gtcaagaact caaccacatc
   113701 gtaacagaac atccatggga tacagctgta gatgaattgg ttatccttgg tgaccacgtt
   113761 acaactgact ctggtacagg tattgtccat acagcccctg gttttggtga ggacgattac
   113821 aatgttggta ttgctaataa tcttgaagtc gcagtgactg ttgatgaacg tggtatcatg
   113881 atgaagaatg ctggtcctga atttgaaggt caattctatg aaaaggtagt tccaactgtt
   113941 attgaaaaac ttggtaacct ccttcttgcc caagaagaaa tctctcactc atatccattt
   114001 gactggcgta ccaagaaacc aattatctgg cgtgcagtac cacagtggtt cgcctctgtt
   114061 tcaaaattcc gccaagaaat cttggatgct attgataaag ttaaattcca tacagaatgg
   114121 ggtaaagtac gtctatataa tatgattcgt gaccgtggtg actgggttat ctctcgtcaa
   114181 cgtgcttggg gtgttccact tccaatcttc tatgcagaag acggtacagc tatcatgaca
   114241 gctgagacta ttgaacatgt ggcgcaactc tttgaagtac atggttcaag catttggtgg
   114301 gaacgtgatg ccaaggacct cttgccagaa ggatttacac atccaggttc tccaaatggg
   114361 gagttcaaga aagaaacaga tattatggat gtatggtttg actcaggttc atcatggaat
   114421 ggtgttttgg taaatcgtcc aaatctgact tatccagctg acctttacct tgaaggttct
   114481 gaccaatacc gtggttggtt caactcatca ctcattacat ctgttgccaa ccatggcgta
   114541 gcaccttaca aacaaatctt gtcacaaggt tttgcccttg atggtaaagg tgagaagatg
   114601 tctaaatctc ttggaaatac cattgctcca agcgatgttg aaaaacaatt cggtgctgaa
   114661 atcttgcgtc tctgggtaac aagtgttgac tcaagcaatg acgtgcgtat ctctatggat
   114721 attttgagcc aagtgtctga aacttaccgt aagattcgta acactcttcg tttcttgatt
   114781 gccaatacat ctgactttaa cccagctcaa gatgtagtcg cttacgataa acttcgttca
   114841 gttgataagt acatgactat tcgctttaac cagcttgtca agaccattcg tgatgcctat
   114901 gcggactttg aattcttgac gatctacaag gccttggtga actttatcaa cgttgacttg
   114961 tcagccttct accttgattt tgccaaagat gttgtttaca ttgaaggtgc caaatcactg
   115021 gaacgccgtc aaatgcagac tgtcttctat gacattcttg tcaaaatcac caaactcttg
   115081 acaccaatcc ttcctcacac tgcggaagaa atctggtcat atcttgagtt tgaaacagaa
   115141 gacttcgtcc aattgtcaga attaccagaa gttcaaactt ttgctaacca agaagaaatc
   115201 ttggatacat gggcagcctt catggacttt cgtggacaag cacaaaaagc cttggaagaa
   115261 gctcgtaatg caaaagttat cggtaaatca cttgaagcac acttgacagt ttatccaaat
   115321 gaagttgtga aaactctact cgaagcagta aacagcaatg tagcacaact tttgatcgtg
   115381 tctgagttga ccatcgcaga aggaccagct ccagaagctg cccttagctt cgaagatgta
   115441 gccttcacag ttgaacgtgc tgctggtgaa gtatgtgacc gttgccgtcg tattgaccca
   115501 acaacagcag aacgcagcta ccaggtagtt atctgtgacc actgtgcaag catcgtagaa
   115561 gaaaactttg tggaagcagt cgcagaagga tttgaagaga aataagattg aaaagtctag
   115621 gcaaaattca atttgagaag aaaagacaac taattttata gtctattaaa cgcattgtat
   115681 cacgtttttg aacacctgat acgatgcgtt ttttatttat tttaaaaatt tgcgaggtat
   115741 gactttttat actcaacaag aatcaaagag aaacttagca agctaacagt agtaagataa
   115801 aataggaatt tgatattagg gataagattg ctaaata
//
LOCUS       DBEROU010000002        86491 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.002, whole genome shotgun sequence.
ACCESSION   DBEROU010000002 DBEROU010000000
VERSION     DBEROU010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 86491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.002"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..109
                     /locus_tag="ACL8CL_000113"
     CDS             <1..109
                     /locus_tag="ACL8CL_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGS1329440.1"
                     /translation="EDVMNQLQDVIQGLEKEVIKSIVNRRWTRMLFESR"
     gene            complement(226..573)
                     /locus_tag="ACL8CL_000114"
     CDS             complement(226..573)
                     /locus_tag="ACL8CL_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1329441.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAALVLDFYRMK"
     gene            complement(684..2459)
                     /gene="spxB"
                     /locus_tag="ACL8CL_000115"
     CDS             complement(684..2459)
                     /gene="spxB"
                     /locus_tag="ACL8CL_000115"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047112 - pyruvate oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HGS1329442.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAVSKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            complement(2666..4909)
                     /locus_tag="ACL8CL_000116"
     CDS             complement(2666..4909)
                     /locus_tag="ACL8CL_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGS1329443.1"
                     /translation="MTEIVKASLENGIQKIRIRSEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFFCGMKMQKGSYTVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDVIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFQTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKKNAKLAISQLKKRGLGTVMLTGDNAGVARAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            complement(4919..5290)
                     /locus_tag="ACL8CL_000117"
     CDS             complement(4919..5290)
                     /locus_tag="ACL8CL_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HGS1329444.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKNPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHTNLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            complement(5301..5696)
                     /locus_tag="ACL8CL_000118"
     CDS             complement(5301..5696)
                     /locus_tag="ACL8CL_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HGS1329445.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTVQTLL
                     ARLVEKECLTRKKEGKSFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     regulatory      5880..5978
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6062..6853
                     /gene="thiD"
                     /locus_tag="ACL8CL_000119"
     CDS             6062..6853
                     /gene="thiD"
                     /locus_tag="ACL8CL_000119"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGS1329446.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGVAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSSARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCTFAAVI
                     TAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     gene            complement(7064..7696)
                     /gene="thiE"
                     /locus_tag="ACL8CL_000120"
     CDS             complement(7064..7696)
                     /gene="thiE"
                     /locus_tag="ACL8CL_000120"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGS1329447.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(7689..8492)
                     /locus_tag="ACL8CL_000121"
     CDS             complement(7689..8492)
                     /locus_tag="ACL8CL_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGS1329448.1"
                     /translation="MQEFTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRIAASYAAQVCLPMVVDAVGVTASSIRKS
                     LVRDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYHG
                     MSFLVTGPKDLVVSKNQVAVLGNGCAELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            complement(8492..9016)
                     /gene="thiW"
                     /locus_tag="ACL8CL_000122"
     CDS             complement(8492..9016)
                     /gene="thiW"
                     /locus_tag="ACL8CL_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HGS1329449.1"
                     /translation="MRKHQLQVHKLTILSMMTALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFSERID"
     gene            complement(9021..9713)
                     /gene="tenA"
                     /locus_tag="ACL8CL_000123"
     CDS             complement(9021..9713)
                     /gene="tenA"
                     /locus_tag="ACL8CL_000123"
                     /EC_number="3.5.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050334 - thiaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HGS1329450.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKELEINDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            complement(9724..10374)
                     /locus_tag="ACL8CL_000124"
     CDS             complement(9724..10374)
                     /locus_tag="ACL8CL_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HGS1329451.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQKLHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            complement(10376..11761)
                     /locus_tag="ACL8CL_000125"
     CDS             complement(10376..11761)
                     /locus_tag="ACL8CL_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1329452.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMESLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMDRTTCEFGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGSVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELQLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLRHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            complement(11762..12322)
                     /locus_tag="ACL8CL_000126"
     CDS             complement(11762..12322)
                     /locus_tag="ACL8CL_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1329453.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGITLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     regulatory      complement(12584..12672)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(12881..13510)
                     /gene="thiE"
                     /locus_tag="ACL8CL_000127"
     CDS             complement(12881..13510)
                     /gene="thiE"
                     /locus_tag="ACL8CL_000127"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HGS1329454.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALRGGITLFQFREKG
                     EGALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIKNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGVKLMRKLL
                     PQMPLVAIGGIQTKHIKDIMKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     gene            complement(13512..14294)
                     /gene="thiM"
                     /locus_tag="ACL8CL_000128"
     CDS             complement(13512..14294)
                     /gene="thiM"
                     /locus_tag="ACL8CL_000128"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HGS1329455.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGASPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPVVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            14396..14578
                     /locus_tag="ACL8CL_000129"
     CDS             14396..14578
                     /locus_tag="ACL8CL_000129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329456.1"
                     /translation="MKIKEQTRKLTADCSKHCFEVVDRTDEVSNHTYGKVKLTWFEEI
                     FEESYLIKFVNIMSLL"
     gene            complement(14575..15243)
                     /locus_tag="ACL8CL_000130"
     CDS             complement(14575..15243)
                     /locus_tag="ACL8CL_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HGS1329457.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYWAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     regulatory      complement(15458..15546)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(15800..16936)
                     /gene="lctO"
                     /locus_tag="ACL8CL_000131"
     CDS             complement(15800..16936)
                     /gene="lctO"
                     /locus_tag="ACL8CL_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HGS1329458.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKDEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     gene            complement(17560..19050)
                     /gene="lysS"
                     /locus_tag="ACL8CL_000132"
     CDS             complement(17560..19050)
                     /gene="lysS"
                     /locus_tag="ACL8CL_000132"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HGS1329459.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLITKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            complement(19190..19324)
                     /locus_tag="ACL8CL_000133"
                     /pseudo
     CDS             complement(19190..19324)
                     /locus_tag="ACL8CL_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
     gene            19540..20220
                     /locus_tag="ACL8CL_000134"
     CDS             19540..20220
                     /locus_tag="ACL8CL_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1329460.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLETISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
     gene            20180..20857
                     /locus_tag="ACL8CL_000135"
                     /pseudo
     CDS             20180..20857
                     /locus_tag="ACL8CL_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154125.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            20858..21615
                     /locus_tag="ACL8CL_000136"
                     /pseudo
     CDS             20858..21615
                     /locus_tag="ACL8CL_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            21627..21869
                     /locus_tag="ACL8CL_000137"
     CDS             21627..21869
                     /locus_tag="ACL8CL_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HGS1329461.1"
                     /translation="MKLFKPLLTVLALAFALIFITACSSGGNAGSSSGKTTAKARTID
                     EIKKSGELRIAVFGDKKPFGYVDNDGSYQGYATILN"
     gene            21911..22492
                     /locus_tag="ACL8CL_000138"
     CDS             21911..22492
                     /locus_tag="ACL8CL_000138"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGS1329462.1"
                     /translation="MSVDAANRAEYLISNKVDITLANFTVTDERKKQVDFALPYMKVS
                     LGVVSPKTGLITDVKQLEGKTLIVTKGTTAETYFEKNHPEIKLQKYDQYSDSYQALLD
                     GRGDAFSTDNTEVLAWALENKGFEVGITSLGDPDTIAAAVQKDNQELLDFINKDIEKL
                     GKENFFHKAYEKTLHPTYGDAAKADDLVVEGGH"
     gene            complement(22572..23219)
                     /locus_tag="ACL8CL_000139"
     CDS             complement(22572..23219)
                     /locus_tag="ACL8CL_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329463.1"
                     /translation="MNEIIILKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(23216..23914)
                     /locus_tag="ACL8CL_000140"
     CDS             complement(23216..23914)
                     /locus_tag="ACL8CL_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329464.1"
                     /translation="MGKLSSHMWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFMEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDSLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFQ
                     SIFYFTRQKRRFIE"
     gene            complement(23924..24675)
                     /locus_tag="ACL8CL_000141"
                     /pseudo
     CDS             complement(23924..24675)
                     /locus_tag="ACL8CL_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24675..25439)
                     /locus_tag="ACL8CL_000142"
     CDS             complement(24675..25439)
                     /locus_tag="ACL8CL_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329465.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKKYLAIVG
                     STVIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGTVFSIFGLASALLVKKKIGAIFIPVAYMMVGGIFWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            complement(25535..25657)
                     /locus_tag="ACL8CL_000143"
     CDS             complement(25535..25657)
                     /locus_tag="ACL8CL_000143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329466.1"
                     /translation="MYYNGGGDNYIGSGTRLAMAPQFGNHVRIHTSGSWNPDSY"
     gene            complement(26118..26750)
                     /gene="pyrE"
                     /locus_tag="ACL8CL_000144"
     CDS             complement(26118..26750)
                     /gene="pyrE"
                     /locus_tag="ACL8CL_000144"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HGS1329467.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFTDAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(26784..27485)
                     /gene="pyrF"
                     /locus_tag="ACL8CL_000145"
     CDS             complement(26784..27485)
                     /gene="pyrF"
                     /locus_tag="ACL8CL_000145"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HGS1329468.1"
                     /translation="MREHRSIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKILSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIHYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGVAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHAIKDEWTQDWN"
     gene            complement(27718..28917)
                     /gene="divIB"
                     /locus_tag="ACL8CL_000146"
     CDS             complement(27718..28917)
                     /gene="divIB"
                     /locus_tag="ACL8CL_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HGS1329469.1"
                     /translation="MSKDKKNEDKETLEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEIEESES
                     KEKEEQDKKLAKKAIKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKAAIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVIDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(28927..29985)
                     /locus_tag="ACL8CL_000147"
     CDS             complement(28927..29985)
                     /locus_tag="ACL8CL_000147"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HGS1329470.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLAKVEHVGA
                     VTKVSDKNTPEPDELVDIQTHFNPKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELRQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(29989..31341)
                     /gene="murD"
                     /locus_tag="ACL8CL_000148"
     CDS             complement(29989..31341)
                     /gene="murD"
                     /locus_tag="ACL8CL_000148"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HGS1329471.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQIASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRDVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDITGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYELATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(31445..32086)
                     /locus_tag="ACL8CL_000149"
     CDS             complement(31445..32086)
                     /locus_tag="ACL8CL_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329472.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTLL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIESQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(32088..33506)
                     /locus_tag="ACL8CL_000150"
                     /pseudo
     CDS             complement(32088..33506)
                     /locus_tag="ACL8CL_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
     gene            33673..35019
                     /locus_tag="ACL8CL_000151"
     CDS             33673..35019
                     /locus_tag="ACL8CL_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HGS1329473.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTGYGTDYACKELSADVYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSRLGDLSLPCPQDENLTILPHSAYSET
                     LYQAGSWSHKRRVCQFSERKEGNLFYDVISLVTNMTSGTSQDQFQLYRGRGQAENFIK
                     EMKEGFFGDKTDSSTLIKNEVRMMMSCIAYNLYLFFKHLAGGDFQTLTIKRFRHLFLH
                     VVGKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEPPGRKASLMMH"
     gene            complement(35209..35817)
                     /locus_tag="ACL8CL_000152"
                     /pseudo
     CDS             complement(35209..35817)
                     /locus_tag="ACL8CL_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
     gene            complement(35888..36187)
                     /locus_tag="ACL8CL_000153"
                     /pseudo
     CDS             complement(35888..36187)
                     /locus_tag="ACL8CL_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
     gene            complement(36551..36805)
                     /locus_tag="ACL8CL_000154"
     CDS             complement(36551..36805)
                     /locus_tag="ACL8CL_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HGS1329474.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
     gene            complement(36825..38666)
                     /gene="typA"
                     /locus_tag="ACL8CL_000155"
     CDS             complement(36825..38666)
                     /gene="typA"
                     /locus_tag="ACL8CL_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HGS1329475.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            38828..39553
                     /locus_tag="ACL8CL_000156"
     CDS             38828..39553
                     /locus_tag="ACL8CL_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS1329476.1"
                     /translation="MRLDNLLAQEKVSRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQAFQKGIVFLDGTVC
                     KPAKLEILSASPSLSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     EGNYRPLNQKELQIIKNYLEMSR"
     gene            complement(39593..39820)
                     /locus_tag="ACL8CL_000157"
     CDS             complement(39593..39820)
                     /locus_tag="ACL8CL_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329477.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(39909..40289)
                     /locus_tag="ACL8CL_000158"
     CDS             complement(39909..40289)
                     /locus_tag="ACL8CL_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGS1329478.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            complement(40289..40558)
                     /locus_tag="ACL8CL_000159"
     CDS             complement(40289..40558)
                     /locus_tag="ACL8CL_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HGS1329479.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            40622..41530
                     /locus_tag="ACL8CL_000160"
     CDS             40622..41530
                     /locus_tag="ACL8CL_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGS1329480.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            complement(41584..42339)
                     /locus_tag="ACL8CL_000161"
     CDS             complement(41584..42339)
                     /locus_tag="ACL8CL_000161"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS1329481.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(42360..43289)
                     /gene="rnz"
                     /locus_tag="ACL8CL_000162"
     CDS             complement(42360..43289)
                     /gene="rnz"
                     /locus_tag="ACL8CL_000162"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HGS1329482.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAKKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            complement(43304..43927)
                     /locus_tag="ACL8CL_000163"
     CDS             complement(43304..43927)
                     /locus_tag="ACL8CL_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HGS1329483.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTEQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKSFIRLNLSGKSFGFCY
                     ESDGFGRIFSENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVVSLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            complement(43920..45158)
                     /gene="hflX"
                     /locus_tag="ACL8CL_000164"
     CDS             complement(43920..45158)
                     /gene="hflX"
                     /locus_tag="ACL8CL_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HGS1329484.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQLKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            complement(45151..46035)
                     /gene="miaA"
                     /locus_tag="ACL8CL_000165"
     CDS             complement(45151..46035)
                     /gene="miaA"
                     /locus_tag="ACL8CL_000165"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HGS1329485.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVLHHLIDVREITESYSAFDFVSEAKMTIEDIHSRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQETLYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRREQTLEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            46156..46326
                     /locus_tag="ACL8CL_000166"
     CDS             46156..46326
                     /locus_tag="ACL8CL_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002263068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HGS1329486.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            complement(46374..47213)
                     /locus_tag="ACL8CL_000167"
     CDS             complement(46374..47213)
                     /locus_tag="ACL8CL_000167"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HGS1329487.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLKTNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLTQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(47310..48269)
                     /locus_tag="ACL8CL_000168"
     CDS             complement(47310..48269)
                     /locus_tag="ACL8CL_000168"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HGS1329488.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KSLGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENTFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            complement(48396..49274)
                     /locus_tag="ACL8CL_000169"
     CDS             complement(48396..49274)
                     /locus_tag="ACL8CL_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ltp family lipoprotein"
                     /protein_id="HGS1329489.1"
                     /translation="MNKRLFLKMSLVTLPILALFSQPVLAEENIHFSSCKEAWANGYS
                     DLHEGEPGYSAKLDRDHDGVACELKNAPKGTFKAKQAATTQTDTTSSTASGWVKQDGS
                     WYYFDGNGNLVKNAWQGAYYLKSNGKMAQGEWVYDSSYQAWYYLKSDGSYARNAWQGA
                     YYLKSNGKMAQGEWVYDATYQAWYYLKSDGSYAYSTWQGNYYLKSDGKMAVNEWVDGG
                     RYYVGADGVWKEGQASTASSSNDSNSEYSAALGKAKSYNSLFHMSKKRMYRQLTSDFD
                     KFSNDAAQYAIDHLDD"
     gene            complement(49324..49941)
                     /locus_tag="ACL8CL_000170"
     CDS             complement(49324..49941)
                     /locus_tag="ACL8CL_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS1329490.1"
                     /translation="MKLIFLHGLGQSAESWKEVRNLLTDYPSEAIELFPSGVSNYQQA
                     KERVYQYLAQETEPFVLIGLSLGAALALELSSYDLPNLRALILSGCPLKLAGNILFYL
                     QLLIFKLLPKRVFEKQGADKALMVGVSEELKTLDLTDIAGTCPYPTLLICGIKDKPNL
                     SSMRSLHKLISESQFQIISDGPHVLNKAKPKEFVEKTRSFLELLK"
     gene            complement(49942..51663)
                     /gene="pabB"
                     /locus_tag="ACL8CL_000171"
     CDS             complement(49942..51663)
                     /gene="pabB"
                     /locus_tag="ACL8CL_000171"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HGS1329491.1"
                     /translation="MHRKTVIDFRTLGERYTFIQPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDRVETSPIPLTYEDIDLP
                     SNWQEVTSAENYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLSANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     KIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     KHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANQDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELSLEEN"
     gene            complement(51724..57276)
                     /locus_tag="ACL8CL_000172"
     CDS             complement(51724..57276)
                     /locus_tag="ACL8CL_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000472960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGS1329492.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMDGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKEEAPRTEESPKEEP
                     KSEVKPTDDTLPKVEEGKEDSVESAPVEEVGGAVDSKTDEQAPVKSESQPSDKPAEES
                     KVEPSVEPVAPREDEKAPTAPVEPEKQPEVPEETVEPKEETKTAKGTQEKGKEGQAPV
                     QEVHPEYKVTTGTVEKSIESELDFTTDVVPDDTKYVGDEVVENPGSKGVQVTKTTYET
                     VEGVETDKVLSTSTEVTKEPVNQQVIRGTKPIEGTLIEESLEKIPFKEVVKEDDQLKK
                     GLEVVAQEGKEGQKKITKTFNTIKGVKTEDAPKVTEEILEEPQDKILKRGTKSFEKPV
                     LTITEIESKDLKRTSDVKYSLENPSKAAIKSITLTLKKGDEIVKTLNVSPEDLTTTLT
                     DLQYYKDYKLETKMVYDRGEGDEEEVLKEEPLRIDLKKVEIKDIIRTDLIKYENQVET
                     DETRLTSVPTDLSNYYLKVTSNDHKTNLLAVQKIEESTIDGKQVYKVIAVADNLVQRN
                     VDNRFEETYTYYIEKPKASQAGIFYDFEELIQAIQTNPAGEFKLGQSMSARNVVPKGK
                     SYITSEFTGKLLSDGDKRYAIHNLEHPLFDKIKAGTVKNINFENIDIDLSNQDNIAAI
                     ANEISGTTRIENIKAIGTITARNNVAGFVNNIKEDATLENVAFIGSINSAGNNSTVGG
                     IAGSNYMGFVNRAYVDATITANNANASMLVPFVQYKAHSWLSGTKGKLTNSVAKGILD
                     VKNTKYVGGITSKTWPYGAVQDNVTYAKVIKGQEIFGSNDVNDGDANPFVSNLFGVIG
                     YSSSEDGTGKDNKNKNKLKHLSKSEADAKVASFNITAAKFVSEPYELNKLNNVSSREA
                     AYESIQDYNADYKLAYKNIEKLQPFYNKDFIVNQANKLDKNNKLNTKEILSVTPMNNN
                     NFVTDLGDANKIIVHYADGTKDYFNLSSSDEGLSNVREYTITDLGIKYTPNIVQKDHS
                     ALINGIVNILSPIELQSNPIYQKLGRTGSNRVNAIKDLYLEESFDHVKANLNILVTKL
                     VENEDHQLNNSSAAQQMILDKVEKNKAALLLGLTYLNRYYGVKFGDVNIKELMLFKPD
                     FYGKNVSVLDRLIEIGSREDNIKGHRTYDAFSEIISKTTLSENLVNFLNYNRRLFTAD
                     VNIYDWFKKATEGNVYIAERASEVDEIKAAKYKVWDNLQSATHRKMILPLLNLNKSHV
                     YLISNYSAMAVGTAEKLGHSSFDGIKNQIEKAADSYRNYFDFWYRLSLDKVKNMLVKN
                     TVTPLWEGYQAPGGWPDKYGKRNNKEDYAPLRELFGPIGKYYGNNGTGAYAVIWDNPL
                     DTRTEVNYIALSMIDEFGISVYTHETTHVNDRAIYLGGYGRRSGTHAEAYAQGMLQTP
                     VPSTWFDEYGALGINMTFYRPNDGNQWYITDPKTLKTREDIDNYMKGYIDTLSLLDYV
                     EGDAVISKHDDKLNQLWFKKVDPIERTVGSKDQYDKVRNMSSDDWKGITLNSVNDLVD
                     NNLMTNRMMNNNIYKPEVFDPGIAYVGIKMMNSIYAGNTSEGAPGAVTFKNNAFRLWG
                     YYGYDKGFLGYASDKLKSQANKEGKQFGDQYIIQKISDGRFQSFADFKKEYYKELLEK
                     ANAGFTPFTIDGETIHNYDELKSLFAKALEEDMVTNKVNGKLVQVKEKVYKKLLQQTN
                     SFKTSIFK"
     gene            complement(57298..57861)
                     /locus_tag="ACL8CL_000173"
     CDS             complement(57298..57861)
                     /locus_tag="ACL8CL_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HGS1329493.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILGSRGIHLAVQGCEHVNRALVVERQVAEQFDLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            complement(57977..59668)
                     /locus_tag="ACL8CL_000174"
     CDS             complement(57977..59668)
                     /locus_tag="ACL8CL_000174"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1329494.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIETGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VAFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFEANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            complement(59665..60402)
                     /locus_tag="ACL8CL_000175"
     CDS             complement(59665..60402)
                     /locus_tag="ACL8CL_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS1329495.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            complement(60625..61737)
                     /gene="msrB"
                     /locus_tag="ACL8CL_000176"
     CDS             complement(60625..61737)
                     /gene="msrB"
                     /locus_tag="ACL8CL_000176"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGS1329496.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            complement(61748..62314)
                     /locus_tag="ACL8CL_000177"
     CDS             complement(61748..62314)
                     /locus_tag="ACL8CL_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HGS1329497.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            complement(62334..63044)
                     /gene="ccdA2"
                     /locus_tag="ACL8CL_000178"
     CDS             complement(62334..63044)
                     /gene="ccdA2"
                     /locus_tag="ACL8CL_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGS1329498.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKGLYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            complement(63255..64115)
                     /locus_tag="ACL8CL_000179"
     CDS             complement(63255..64115)
                     /locus_tag="ACL8CL_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HGS1329499.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLCFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQLLFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            complement(64294..66348)
                     /locus_tag="ACL8CL_000180"
     CDS             complement(64294..66348)
                     /locus_tag="ACL8CL_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HGS1329500.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRAYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKDKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYRRSLISFMQESRLRETAIISSGAFVERVINRVKPNNID
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            complement(66360..66614)
                     /locus_tag="ACL8CL_000181"
     CDS             complement(66360..66614)
                     /locus_tag="ACL8CL_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329501.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERSVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            complement(66607..67164)
                     /locus_tag="ACL8CL_000182"
     CDS             complement(66607..67164)
                     /locus_tag="ACL8CL_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1329502.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            complement(67148..68104)
                     /locus_tag="ACL8CL_000183"
                     /pseudo
     CDS             complement(67148..68104)
                     /locus_tag="ACL8CL_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            68196..68555
                     /locus_tag="ACL8CL_000184"
     CDS             68196..68555
                     /locus_tag="ACL8CL_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HGS1329503.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            68933..69010
                     /locus_tag="ACL8CL_000185"
     CDS             68933..69010
                     /locus_tag="ACL8CL_000185"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329504.1"
                     /translation="MLVSTLLFAYSQGIFSGRKIEKWKS"
     gene            69391..69621
                     /locus_tag="ACL8CL_000186"
     CDS             69391..69621
                     /locus_tag="ACL8CL_000186"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1329505.1"
                     /translation="MNERYQNLKAKECQALLSPQDRQIFAQRKIDVEPVFGQIKACLG
                     YKRCNLRGKRQVRIDMGLVLMANNLLKHSEMK"
     gene            complement(69869..76570)
                     /gene="bgaA"
                     /locus_tag="ACL8CL_000187"
     CDS             complement(69869..76570)
                     /gene="bgaA"
                     /locus_tag="ACL8CL_000187"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000515226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adhesin/beta-galactosidase BgaA"
                     /protein_id="HGS1329506.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSQGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSL
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTNLNSVDKSVSYVLTD
                     GSVQEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPVVPTV
                     TVGGEAVTGLTSRQPMQYRTLSYGAQLPEVTASAENADVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQIFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAKLKAGEMNHF
                     SFDKVETYAVRIRMVKADNKRGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDSYETKDLTVEWEEVPAEN
                     LTKAGQFTIRGRVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(76658..76894)
                     /locus_tag="ACL8CL_000188"
     CDS             complement(76658..76894)
                     /locus_tag="ACL8CL_000188"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329507.1"
                     /translation="MKIKEQTRKLAAGCSKHSFEVVDKTDEVSSKHGFEVVDETDEVS
                     SKHGFEVVDETDEVSNHIYGKATLTKFELDFRRV"
     gene            complement(76920..78395)
                     /locus_tag="ACL8CL_000189"
     CDS             complement(76920..78395)
                     /locus_tag="ACL8CL_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HGS1329508.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVVFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            complement(78443..78748)
                     /locus_tag="ACL8CL_000190"
     CDS             complement(78443..78748)
                     /locus_tag="ACL8CL_000190"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1329509.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            complement(78786..79262)
                     /locus_tag="ACL8CL_000191"
     CDS             complement(78786..79262)
                     /locus_tag="ACL8CL_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1329510.1"
                     /translation="MVLDYFFDKNLVFCLEADNQEQLFDQVASLLEEREIVTPTYREA
                     LITREKSFPTGLDMEFLGKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDK
                     EVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDLSKISEPEALYAYIAEATA"
     gene            complement(79889..86308)
                     /locus_tag="ACL8CL_000192"
     CDS             complement(79889..86308)
                     /locus_tag="ACL8CL_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HGS1329511.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     AENKEKHEDIHSAMETSQDTEEKKTTIIEEKEVVSKNPVIDTKTSNEEAKIKEENSNK
                     SQGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKELSSLKNTKVLYTYDRIF
                     NGGAIETTQDNLNKIKQIEGITSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFG
                     KNFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNYWLSDKIPHAF
                     NYYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSY
                     KMYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAG
                     IPMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFD
                     KVNIGGQSFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKI
                     AVMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQV
                     FSISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKE
                     IDFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKST
                     YGYMSGTSMATPIVAASTVLIRPKLKEMLEKPVLKNLKGDDKIDLTSLTKIALQNTAR
                     PMMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLK
                     EIKGDKKYFTIKLHNTSNRPLTFKVSASAVTTDSLTDRLKLDETYKDEKSPDGKQIVP
                     EIHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEME
                     ALSSNGKKTDFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSKSKTMEGYDDDGKPKIP
                     GTLNKGIGGEHGIDKFNPAGVIQNRKDKNRTSLDQDPDLFAFNNQGVHAESTSGSKIA
                     NIYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQKDLKVVSREHF
                     IRGILNSKGNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQ
                     FEPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTY
                     HKVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQ
                     GRNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFANQADEKGMISY
                     YLVDPDQDSSKYQKLGEIPESKFKNLKNVKDDSLNKETAEVENNLLVDNQSIEGKSLF
                     NIHKTISTIRDFENKDLKKLIKKKYKQEDDFVNGGTRTVERDYKYDDKGNIIAYDDGT
                     DLEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKSIEIKATK
                     YDFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRMPEKIKETKSEY
                     PYVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKV
                     TTYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRVLSETTIYADSRNVEDGRSTQAVLM
                     SALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEA
                     IKEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVN
                     ESVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYL
                     QTGYSNLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFD
                     GEIQEQHIYINGKEYTSFDDIKQITDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSE
                     LKPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYV
                     INLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDH
                     SQKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLG
                     LKKKNQD"
BASE COUNT        25344 a        18796 c        15507 g        26844 t
ORIGIN      
        1 ggaagatgtc atgaatcaac ttcaagatgt catacaagga ctggagaagg aggtgataaa
       61 gtccatcgtt aatcggagat ggactagaat gctttttgaa agcagatgag tattatatgc
      121 aatttcttta tataaaaaga ccggattgct ccgatctttc aatagttcat attctcaatt
      181 tctattttaa aaatagctaa ggttaacgtc aaatgactac gcgacctatt tcatacgata
      241 aaaatcaagc actagagcag caggtccttg aactaataag gactctgttc cccaatcggt
      301 tacagttggt ccgtgtaaaa cctttatacc aagctcgttc aaccgtttgt agttctggtc
      361 tacatcctca acctcgatat gaataatgat tcctgactga aagttttcca aaggaaccaa
      421 atgattttgt gacaacataa ggcagtgact accaatcgta aactgagcaa aaccatcatc
      481 agcataatct gcctttttat ccaagatatg ctccaagtca gcacagactt ggggaacatt
      541 tgaaacgata atatctaatt gatttaaatt catttactct cctccataaa aagaccggat
      601 tgctccgatc ttttaaagtt ctgctctatg aaaatcaaag aataaagtct acaagtttca
      661 tatttgattt tcggcgagag gaattattta attgcgcgtg attgcaatcc ttcttcttcc
      721 aagaagagac ggaatggtac gagttcttct gcttcgtatt tttccttgaa ggctttgata
      781 gcttcttctg agtgaagttt tggatccaat tcaagtactt ctactggaag tggacggtgt
      841 tgagtgatgc gagcatcgat gacaacagtt ttaccttctt tgttcaattt aacagcttct
      901 gcaacaactg catcgatgtc ttcgatacgg tcaactgtga atccaacagc tccttgagct
      961 tccgcaattt tagcgtagtc agcgtttgtg aagtctacac caaacaagtg tttgtttgta
     1021 tcttcgtatt tgttcttgat gaagccgtac tcagcatttg agaagacaag gttgataact
     1081 ggaaggtcgt attgaacgtt tgtgataacg tctgggtagc acatgttgaa tgctccgtca
     1141 cccatgatgt tccatacttg gcgatctgga ttgtctttct tagcagcgat accaccagga
     1201 agggcaatac ccattgtcgc aaagagtgga gatgtacgcc acatgttctt aggtgtcatg
     1261 tgaaggtgac gagtagatgt ttgagtagtg ttacctacgt cgattgagta gatagcgtct
     1321 tgatcagcat gtttgttgat tgcattgtaa acttgataca attgcaattc accctcagtt
     1381 ttaccttcga gtttgttcat gtaatcacgc cagttttggt tgttcttaac gtttgcacgc
     1441 caccatggag ttgattcaac tgggtttact ttgtcaagga tagctttagc tgcttgacca
     1501 gcatcaccaa ggattgaagc gtcaagggca tgacgtttac caagtttgta agggtcgata
     1561 tcgacttgga taaatttttc agtgttcttg aatgcttcgt aaacttcagc aaatgggaag
     1621 tttgaaccaa ggaaaagaac tgtgtctgct tcaaagacca cttcgttggc tggtttccaa
     1681 ccaacacggt aagcagaacc tgtcaaacct tcatagttcc attcgaaagc ttcaaagttt
     1741 ttaccagttg tgatgattgg tgctttgatt ttacgtgaca attcagtaat cacttcacca
     1801 gctttaacac caccaaatcc agcatagata actgggcgtt cagcattgtt caagatttca
     1861 acagctttgt cgatttcaac ttcgttcaaa gcaggagcga tgaatgagcg ttcgtatgaa
     1921 cctgaaccgt agtatgagtt ttcgtcgatt tcttggaaac cgaagtttac tggaatttca
     1981 acaacagctg gacctttttt agaaactgca gcacggcagg cttcgtcaat tacttttggc
     2041 aattgctcag cgtaagctac acgtttgttg taaacagcga taccgttgta cattgggttt
     2101 tggttaagct cttggaaagc atccatgttc aattcgttaa ctggacgtga tccaaggatt
     2161 gctaggaatg gagtgttatc catagctgca tcgtaaacac cgttaatcaa gtgagtcgca
     2221 cctggaccac ctgaaccaac tgcaaccccg attgagccgc cgaatttagc ttgcataacc
     2281 gctgcaagag cacctgtctc ttcgtggcga acttgtaaga agcggatatc tttgtcttca
     2341 gccaaagcgt ccatcaatga gctgagtgtt cctgatggga taccgtagat tgtatctacg
     2401 ccccatgttt tcaatacgtt aagcattgct gcagatgcag taattttccc ttgagtcata
     2461 atgataactc tccttcaatt tttttagact tggagaatac gattacatag aattggaaac
     2521 gttctccaaa tttttactat tccactgtat catatttatg ctgacttttc taaaaatctg
     2581 ctcaaaactc tctattctct attctaatac agttttgaaa gtgtcgtcat ttctgtttta
     2641 taacaaaaaa atctagtcat tacttttagt ctattttact aaaatttaac agaagggaac
     2701 tggtcagaac agatacagaa ctaaaggcca tggctagacc tgccaattct gggttgagag
     2761 ccagtccaac acctgaaaag actcctgctg caatcggaat tccgacaaca ttgtagataa
     2821 aagcccagaa aagattgagc agaatacgat gaaaggtttt cttactcatg tcgaaggcac
     2881 gcaccactcc taaaagatta ttggttgtca acaccaaatc tgctgactcg atggcgatat
     2941 ctgttccagc tcccatagca atccccacat ctgctacact aagggcagga gcgtcattga
     3001 tcccatcacc aacaaaggct actttacctg actgttgcag tttatggatt tcatgggctt
     3061 tttcttctgg caagacgcct gcaatgacct cttcaattcc gatttgatct gcaatagcac
     3121 gcgccacacc agcattgtct cctgtcagca tgactgtccc gagaccacgt ttttttagct
     3181 gactgatggc tagcttagca tttttcttag gaatatcttg caaagcaagc aagcctttga
     3241 tttcattgtc aacagctaag aacacaactg tcttagcttc tttttctagt tcttctagtt
     3301 tatcttgata agtattagaa atatccatgc catccagcat tttagcattt ccaagtaaaa
     3361 cttgttttcc attgattcgc cctgaaacac ctttcccgtg caaggcctga aaattttcaa
     3421 cagtttgaaa ctcaagtcca gcttcactcg ctcgcttaac gatagcctca gccagtgggt
     3481 gttgagaagc atcttccaag gaggctgcca atccaaacac ttctacttcg tcgccgatga
     3541 catctgttac cacaggtttc ccttccgtca aagtcccggt cttatcaaag acaagggttt
     3601 gaactttctg gatttcctgt aagacagttc catttttgag gagaaccccc atcttggcac
     3661 tacgtcctgt ccccaccata agggctgtcg gtgttgcaag tcccaaggca caaggacagg
     3721 cgataatcaa aaccgccact ccgtagagaa gagaggacac aaagctagct ccaagcacaa
     3781 ccacactatc cctgagcaag acgaaccaaa cccaaaaggt catgattcct aaaatgacaa
     3841 ctactgggac aaaaatccct gaaatcttat ccgtcaagtc ctgaatcggc gcacgacttg
     3901 tctgagcttt cttcacaaaa tccacaatct gagccaaaac agtctctgag ccaacttttt
     3961 ctgctctaaa gacaagcgtt ccactatgat tgatggttga gccaatgaca gtatctccaa
     4021 ctgtcttgtc cacaggcaga ctctcacctg tcaccatgga ttcgtcaata ctagagacac
     4081 cttctactac gacaccatca acagcaatct tttcaccggg acgcactcga atcaggtcgc
     4141 ctaccttgac ttgttccaaa ggaacttgga cataactatc atcactcaag acttctgcgg
     4201 ttttagcttg caagtccagt aatttctcca cagcttggga cgtatttttt ctcatttttt
     4261 cctcaaaaac tgctcccaaa agaacgaaaa agaggataaa tccagcactt tcgaagtaaa
     4321 cagggagacc agcaaagaga gcaactaggc tatagaaata agccactaga gttcccagcg
     4381 caaccaaggt atccatgttg gcattgtgct ttttaaaact ggcccaagca ctctggatat
     4441 atggcttacc tgcaactaac ataataggcg ttgttgctag aaaggttccc caatgcatga
     4501 cttgatgact aatgctacct gtcaacatcc caatcatgag aatcacaaga ggcacagtaa
     4561 agatactagt aatccaaaaa cgttgcagga gagatagaga ttttcgagtc ttctcaacga
     4621 ctgtatagct tcccttttgc atcttcatgc cacaaaaaaa ttcatgtcgc cctaattctt
     4681 gaggtgtaaa acgaatgact ttctcctcat ctacgccgat tggttccaag ataccttctt
     4741 cttcaaacag aatttcctta taacagtttg aaggagtagc acgatgaaag gtaatctcag
     4801 ctggaattcc cttttgaagc tggatatggg ctggatgata gcctttttca gatcggatac
     4861 ggattttttg aatgccattt tctaagcttg ctttcacaat ttctgtcata gtctccacct
     4921 actctacaat catcttgccg tgcatcatgt tcataccaca agcaaagcca aattctccag
     4981 cctgttcagg cgtgatttcc actacatact cttcccccat tggcaggttc gtatgtacac
     5041 caaaatctgg aaaaacaatt tgatccagac atggtgaagg atccttgcgg tcaaagacaa
     5101 tgcgggctgg cactgatttc ttgaggacaa tcaactcagg agtatagcct cccatgactt
     5161 ccactcgaat ctcttggtat ccgttttttt gctgggcttt ttgtccagat ttttcaggat
     5221 ttttgaaaaa ccaaaacaag ataaacgcga taagggcaat acaaataatg gttacaatac
     5281 tatttaacat gacgtctcct ttacatacaa ttacatctta cttctgttac agcacttgat
     5341 ttcttctctg aaatcacagc ttccaagtct tccaagtcag tctgagtaaa ttcacattct
     5401 acaatcaagt cagccaacaa attcctaatc ctacgggaac aaaccttgtc tttgatatct
     5461 tggacaagta aatcccgact ttggtctaga gttaaaagcg ctgaataaac aaaggacttg
     5521 ccttcttttt tccgagtcaa acactctttc tcaactagac gagccaaaag tgtctgaacc
     5581 gtggacttgg accagtcaaa ccgctctgcc aaaaccctaa tcaaatctgt actggtctgc
     5641 tccccctgca tccaaataat cttcatgacc tgccattctg catctgaaat ctgcattacc
     5701 atacctccaa aatctacatt tgtcaattac actcaacagt atactcccaa aatctacatt
     5761 tgtcaattat agaaataata ttttcttcga aaaatagaat tttaatcatt tgaaaaacga
     5821 tttgcagtca aatattacta tataaacaat aaaaatatgc tatactaaag aaaaaagaaa
     5881 acaaccacta ggggtgcgta aagctgagat taacgactgt tagatccctc tgactcaatc
     5941 taggtaatgc tagctgatgg aagtggaaat gataatgggg actagcagtc ttctattgcc
     6001 tttctaaaac agactagctt gttcttaaga atacaaactt cagttggttg ggaggtttta
     6061 gatgacttat ttacccgttg ctttgaccat tgcagggact gaccctagtg gtggtgctgg
     6121 cattatggca gatttaaagt cattccaagc gagagatgtc tatggagtgg ctgttgtaac
     6181 cagtcttgtc gctcaaaata ccagaggtgt tcagctaatc gagcacgttt ctcctcaaat
     6241 gttgaaagcc caattggaga gtgtcttttc tgatattcca cctcaggctg taaaaactgg
     6301 aatgttggct actactgaaa tcatggaaat catccaaccc tatcttaaaa aactggattg
     6361 tccctatgtc cttgatcctg ttatggttgc tacaagtgga gatgccttga ttgactcaag
     6421 tgctagagac tatctcaaaa caaacttact acctctagca actattatta cgccaaatct
     6481 tcctgaagca gaagagattg ttggtttttc aatccatgac cccgaagaca tgcagcgtgc
     6541 tggtcgcctg attttaaaag aatttggtcc tcagtctgtg gttatcaaag gcggacatct
     6601 caaaggtggt gctaaagatt tcctctttac caagaatgaa caatttgtct gggaaagccc
     6661 acgaattcaa acctgtcaca cccatggtac tggatgtacc tttgctgcag tgattactgc
     6721 tgaactagcc aagggcaaga gtctttacca ggcagttgat aaggccaagg cctttatcac
     6781 aaaagctatt caagatgccc ctcaactcgg tcatggttct ggtccagtca accatacaac
     6841 ttttaaagat taagaaaaaa aaactctcta gttcccactt taagggaatt agagagtttt
     6901 tatactcttc gaaaatctct tcaaactacg tcagcttcca tctgcaactt caaaacactg
     6961 ttttgagctg acttcgtcag tcttatctaa aacctcaagg cagtactttg agcaacctgc
     7021 gactagcttt ctagtttact ctttgatttt cattgagtat taattaggaa agaatgtcat
     7081 gcaactttgt taaaaaggct tgcgtttttg cctcaatatc ttctgcttgc atcaaatcac
     7141 gtacaacagc tacaccagct atgccagtgc ccataagctg atcaatattc tccgaagtca
     7201 agccaccaat agcaactatt ggaatggcaa ccgtttggca aattgttttc aaggttgaaa
     7261 tcagggtgat gggtgcattt tccttggtgg ttgtcggaaa aatggctcct gtccccaagt
     7321 aatctgcacc tgatttctcc gcttccagag ctcttttaac cgttttagcg gtgacaccga
     7381 ggattttttc aggacccaag acttgtcggg caaccgaaac tgggagttcg tcatctccaa
     7441 tatgcagacc agcagcatca accgcaagac aaacatccaa ccgatcatcg attatcaagg
     7501 gtacctgata agcatctgtt atttccttga cttgttttgc cagttgataa tattgattgg
     7561 ttgtgagatt tttttctcgc aattggacta tggtaacccc tgaacggcag gccgtctcaa
     7621 cttttgcaag aaagctttcc acggaatctt gatagcgatt ggttaccaga tatagtctaa
     7681 gtgcttctct attcataaac ctctcctttg atggtatcta gccaattttc atctcttctt
     7741 aggagcgaaa gctgattgag tacttggtaa cgaaattctt ccaatcccat tccttgaaca
     7801 actattttct cagcagcgat attgagataa gagactgcta agcaagaagc ttcaaaacca
     7861 gtctttcctt ggctgagaaa aacggctgtt aaggctccaa ccaaatctcc tgtccctgtt
     7921 atccagtcta attctgcaca gccatttccc agtacagcga cctgattttt cgaaacgacg
     7981 aggtccttgg gacctgtgac taagaaagac ataccatgat aggtctgaca ccagtctttc
     8041 aagacttgaa gcaaatcctc cgtttcttga tctttagcac tcgcatcgac cccaacgccg
     8101 tggtgcttta atccaacaag acttcgaatt tctgacatgt ttcctttaag gaccgtaggt
     8161 ctatagtcta aaaggtctct gactaagctc ttacgaatgg atgaagctgt tacgccaacc
     8221 gcatctacta ccattgggag acaaacttga gctgcataag aagctgctat acggattgct
     8281 ttctccttct cagctgacaa atgccccaaa ttgatgaaga gagcctgact ttgcttagta
     8341 aaatcaagaa cctcacgggg atcatctgcc atgacaggtt tgcatcccag agccaaaatc
     8401 ccatttgcca gcatctcaca agaaatctca ttggtcatac agtgaatgag ggaactagag
     8461 cctataggaa agggatttgt aaattcctgc atcagtctat cctttcacta aagaaatatc
     8521 cctgcacttt tttaaagaat tcctgcttga ttaaaaatcg aaaggcaata aaggaaatcg
     8581 ctgtaccaat caaggttgct ccgaaaaatc gaggcgtgta gataaaccag ctaagcttag
     8641 cagctgatcc tgtaaagagt accataacag gataggaaac aatagaacca ataatacctg
     8701 ttcccacaat ttctcccaag gcagaaaagt aaaattttcg accgtactta taaaagagac
     8761 ctgctagaag ggctccaaaa gtcgctcctg tgagagctaa aggcggaatc ccttgagtcg
     8821 tcatacggat aaaggctgtc actgtagcca tggccaaggc ataaacaggt cccatcatga
     8881 ttcccgctag aatattgact acactggaca tcggtgccat tccctcaatc cgaaagatag
     8941 gtgtaaggac tacatcaagg gcagtcatca tagataaaat ggtcaatttg tgaacttgta
     9001 gttggtgctt tctcatgttt ctattcttct cctttttcta aagactgtaa atcgctcttc
     9061 catgtctggt gttggtaagc catctcccaa aacttggctt ccatatgaac actgatgtgg
     9121 aaggcatcta gcattttttg cttatctgtc tcatcacttt ctcgatagag ctgattgacc
     9181 agtgctccct cctctctgat ctgttgctct aactcatccg taatataagt ttcaatccat
     9241 tgttgataga gaggatttgg tgatggttta agattaagtg atttgcctat atcatggtat
     9301 aaccaaggac aaggaagcaa gcttgcaaaa gctatggcta agttcggttc tgcaaattgc
     9361 ctataaatat gagaaatgta atgataacag gttggagcga ttggatgttg ctccatttcc
     9421 tggtcgttga tttccaattc cttgaaaaat tgttggcgaa taaataactc accctccact
     9481 aaaccctgag cattttgttt caagagtctt ttcatctctt ggtttgaagt cttatcagcc
     9541 aaaagatgat agatttctga gaaagccttc agatagtagg catcctgaat caggtaatag
     9601 cggaaaatgg caggttctaa attcccctct tgtaattgta aaataaaggg atgatgaaag
     9661 gaagcctgcc aagctttctt ggataattcc atcgcaatat ctgtaaattc cataataact
     9721 cctttataaa aatagactgg tttgaagcaa taaaaagaaa agcaggtaga ttaattttgt
     9781 ttttttagga atataaaaag tccgatagct attcttcaac tgtgcatgtt cgtcatatcc
     9841 gtgagcagat agagctctca ggtaaagatg gcgccaccta aagactgtca tcagaacctt
     9901 actgtaaatc aagggcgacc aaaaatgtag tttttgacca cgtaataggc aagcttcttt
     9961 gagggacttg atttcttgct gaatgagagg aaaagaattg aataccacaa tcaaggcata
    10021 ggaccaagag cgtgatagcc ccttttgagc caagtacaag agaagctctt ttagtgaaat
    10081 agaggaaaca aagacaaggc cgatacaaac tgtcacaaag gccctcgttc caagcatgac
    10141 tgcctgtgaa gcatctccgt gtaactgaac tgcccagtag ttggcaaaag atggtaaaat
    10201 ggcaagtatg atcatccaag ctaacatttt aaatcgacgg taatagagca taaagagaat
    10261 acaaaatgcg actaccgaaa gattcagagc aatcgaagga atgaaagatg tttccaagga
    10321 taaaatcagc aagaagagac tgataatcgg tgtctgggtt gctactttga ccatactatc
    10381 tcacctcccc ttgggtattg ctactctgag atgtaagtgg tttggtaatg gtcacttctt
    10441 tcacatgccg aagaccctga ctagtcatct caatccaata atcaaccaca gaaatcaaag
    10501 ggtctaaacg atgactaatg agcagaaaac ttcttccttg attcctctcc tccacaatcc
    10561 acttgcaaaa ataatggcag gctctatcat ccaaacctgc aaaaggttca tctagcaaga
    10621 tcacggaagc cttactggtc aagatggtca ggagctgaag aattttttgc tgaccaccac
    10681 ttaattgata gggactctta tcgactgcct gctccaaatc aaaatatcgt aaagcttgaa
    10741 aaatccgctg atttctttca gaatcaggtc catctaattg aagctcctct cgcagactga
    10801 ctcggataaa ctgcttctca gcttcctgaa caacaccagt cagatcacga tacaaactct
    10861 ttttcttttt caggaccgaa cccttccatg caatgcgccc cttatacttt tgaaattgaa
    10921 gaatagaccg aaagagggtt gatttcccga caccattgtc acccaggata caggaaatcc
    10981 cttgatagaa tgtgaaatca gcaattgaaa agagggggcg atttccaaac tcacaagtcg
    11041 ttctatccat atggaatagt tccgggctag aagcaacttc ctttgaagca acctgtgtca
    11101 tctcatagga agggatttga aacacttccc ttagttttcc gtctcttagc tcaaccatat
    11161 ggtcgatata ggctttatag tcagataaat catggtcgca caaaataact gtctttccat
    11221 caagagccaa ctcttttaga atctccaata tctcgattct gctcttgcgg tcaatggaag
    11281 caaaaggttc atccaagaga tagaccctag gattcatggc aaagaggaca gccagcgctg
    11341 ctttttgctt ttccccacct gataggtgat gaatgggacg gtgtaagatt gcctcgcaac
    11401 gacattgctg gacaacctct gctattttag aatcaatttc ctgaaggtga tagccgatat
    11461 tctccaaggt gaaaatcagc tcctcaaaca agctctccat ggtaaattga tgattgggat
    11521 tttgaaagag aatgccaacc ttctggacac gtgcgatgat agaaagttga ctgacctcgc
    11581 tcccatctat caggacttga ccactatagg gaagagaact gacttgagca atgacttgaa
    11641 aaaggctgga ttttcctgaa ccactgctcc caactaacaa ggtaaaggct tgcgcatgaa
    11701 aagtaaaatc aaacggctca gagaagattg gggactgaat cgctcgtagt tccagaccca
    11761 tctatgcttt tcctccagtt gcaaactgat gatagagttt gacaatggca cgaaccaaga
    11821 tggtacagaa gaaataaaca gaaataaaac gtaccacaag taaggaaagg acaaacggaa
    11881 gggaaaaggc gtagtaacct aacttaatgt attcatagac aaagctaaca agcgtaatcc
    11941 caatactatt agcagttaga gagagccaac tttcatagcg attcttagtt acgataaaac
    12001 caaattcact tcccaaacct tgaaccaagc cagacaaaag agctcctaga ccaaattggc
    12061 taccataaag gacttcagca agcgcagcta gcacttctcc aatcgttgca cttccgactc
    12121 tcggaacaaa gatggcagca atgggcgcag ccatacacca gagaccgaag aggatttcat
    12181 tggcaaaggc ctgcaaacca agaggtgtta agagtagact gagaatatta tacacatatc
    12241 ctgaaccaac gaaaacccca ccaaaaaaga tagacaagaa agcaagcaag ataacatctt
    12301 ttaactgcca ttttttcaac ataaaaaact ccttttttta aagaaaagtg gggcactcaa
    12361 gaagaccgac ctaaatactt tgtatagcag actgaattta gaacagtaca caagaatgct
    12421 aaaatatttc tagaaattaa tttgaatttt ctaattgatt tgttcgcatc ttatttcaat
    12481 ctactatatc atcttcatcc agtttcgtaa aagaaaaaac tctaattaca gatacgaatt
    12541 agagttcagc ttacaagatt agacagttct tttcgacata cgaaaaaaac atttcacatt
    12601 tcccttcgcc agtcttaact gtatcaggtt caatgggtat catctcagcc taaagcaccc
    12661 caaatgcctt tattatttaa ttatgtgatt attataacac acattttata ctagttcaag
    12721 aaattgaact ggaaatacag ccttgcactc acaaagacag cagatctttc ttttgcaaaa
    12781 aacaaatgac ctgtttgatg aattagctat tcaagctgaa tctagacata gttttttaaa
    12841 aaaggaaaat cctacttact tagaatccaa ggatagatat ctattgttca ctcatttccc
    12901 gaacagtttt ttctatattt tttgcatacg atattgccga aatgattgaa acgccgtcaa
    12961 cattggtctt cataatgtct ttaatatgtt tcgtctgtat cccaccaatt gcaactaagg
    13021 gcatttgtgg caatagtttt ctcatcaatt taacaccttc ataacctata gcaccaccag
    13081 catcatcctt tgactgggta tcaaatacag gaccaacacc aacataatct acatattcaa
    13141 cttttgattg ttgaaattct tcctcgtttt ttatagaaag accaattatt ttatctggca
    13201 tcaattttct aatttcatca acaccaatat catcttgacc tacatgtacg ccatcagcgt
    13261 caatttccat tgccaaatcg atatcatcat taacgataaa cggcacattg tattttttac
    13321 aaagttcttt aatttggata gctagctcaa gtttttctaa gccttctaaa gcaccctcac
    13381 ctttttctcg aaattgaaat aaggttatac cacctcttaa ggcttcctca acgactgtat
    13441 atagattttt tccttggcaa gtagtcgttc cacaaataaa atatagtttt agtaattctt
    13501 tatgaaacat cttacttcac tcttttgaat tcctttacat cttcatctgt aatctcgtat
    13561 aaggcattta taaattcaac tttaaatgtc ccaggaagat gtccatttgg acgtttttct
    13621 gccatttctc cagcgatatt gtaaaccaac attgctgttt ctaatgattt caattcttga
    13681 cctttttcta gtccgataaa gcttgctacc actgccccta ataaacatcc agttccaata
    13741 acttttggca tcatggcact accattatga atcgttacca cttctccatt taccgcaata
    13801 gcatccactt cacctgttac tactattgga atattgaact tctcatttgc tgctagagca
    13861 atctcgtcaa tattatctac tcccgcacta tctactcctt tagatgccac atctattcct
    13921 actaaagagg caatctctcc agcattcccc ctaatcgcag ctagtttgta attgttgatt
    13981 aaatcatctg ctactttttt tctatattct cctgctccac aggctacagg atctaaaact
    14041 actgggacat tatatttctc tgcaattttc agagcagctt ggtataattt ccaattttca
    14101 tctgtcaatg ttcctatgtt tattaataaa ccaccagcat actttaacaa atcctctaaa
    14161 tctgctggaa actcactcat ggctggtgag gcgcccagtg ctactaatcc atttgctgtg
    14221 aaatttttta ctacatcatt ggtgatacaa atgaccaatg gtgctttttc ttttaataat
    14281 tttaaacttg tcatattgaa atccttcctt ttcactttat acgatctact gatttcgatt
    14341 tatttctatc tttcgttaag aatttttttc atttacattg aatgatttat actcaatgaa
    14401 aatcaaagag caaactagga agctaaccgc agattgctca aaacactgtt ttgaggttgt
    14461 ggatagaact gacgaagtca gtaaccatac ctacggcaag gtgaaactga cgtggtttga
    14521 agagattttc gaagagtctt acctcatcaa atttgtaaat atcatgagcc ttctctagac
    14581 atcgtaacca atatcaaaaa aagctaattc taaagcgact gcttgattcc atcgttgctg
    14641 aagttctgtc aaatcttctc gatttttacc gacacgattg agttcgtcaa ccagaaattg
    14701 aacccactct gcaaagaaag gacctctgtg gagattgatc cattccgaat gaatatagac
    14761 ttcaggtaga gccaaatctt tagaacccca gtctaaatag agaccttctg caatgaccag
    14821 cataaccaaa agatgggcat agtctgatga agccactgcc caatacatta gatcctgaaa
    14881 ggcttttgtt acagggtgca aagtcacttc tagatagtca ttctctgcta cttttaactc
    14941 tttaaaagcc ttttggaaat aaccatcttc atctgcttca agaaagccta gttgcttggc
    15001 aaaacgaagc ttggattcaa gtttatctgc gtgagctacg caggcaccca gcatggataa
    15061 gaaggcatca aagaagtgat aatcttgaat cagatagtcc tttaagacct tattctcaat
    15121 tgtccccgca aaaagttcct taacaaaacg atgattgatt gcagcctgcc aatccttctg
    15181 actgcttttt aataattctc caacagtcaa acctggctga aatgcatagt cttgtgtttc
    15241 catatttact tctcctctct ttacttgtta gtaattaata aaacaccaag aaatatcaag
    15301 caaaatcgta attccacttg atccttttaa aacacatcga gagtatttgc agagagctaa
    15361 ctaaacaagc ctatccagtt tatataaaca aaaaactcca attacaatca agaattagag
    15421 ttgacttaca agattagacc gttcatttca ccatacgaaa aaactgttca catttccctt
    15481 cgccagtctt aactgtatca ggttcaatgg gtattatctc agcctaaagc accccaaatg
    15541 tctctattat ttaattactg aaccagtata gcaaaaaatg aaagccctag caagatattt
    15601 gaccgaaaaa tatctttata tataatatat tgaaactgga atagtacacc tctacttcta
    15661 aaacattgtt agaaatcgat ttaactgtcc tgattaattt gtcctattct tatttcattt
    15721 tattatagtt ttcgatagcg atttattctt ccaatacacg aagaaaaacc tccacattca
    15781 gtggaggcaa tctgttttat caatacaatt ttaagtcacg agggtcaact gggaaggttg
    15841 ggttgtatgg attgtgacgg agcttaaagt gtttgacatc ttcaatggtc tgagctccag
    15901 acaattgcat aactgtcttc aattccgcat tcaagtgttc aaagacttga cgcacaccga
    15961 cactaccacc gagagccaag ccataaataa cagggcgacc aatagctacc aagtctgctc
    16021 ctgaagccaa ggctttaaag acgtgttgac cacgacgaac accagagtca aagacaatcg
    16081 gcacacgtct atcaactgct tctgccactt cttgaagcga gtcaaaggca gctggtccac
    16141 cgtcgatttg acgaccaccg tggttggtta cccagatacc agaagctcct gcagcaagcg
    16201 aacgttcaac gtcctcacgg cattgtggtc ccttgacata cacaggaaga ccagagtatt
    16261 cagcgataaa ttctacatcg cgtggagaca agcgttgttt agctgatttg taaacaaagt
    16321 ccattgattt accagcacct tctggcaggt attcttcaac aatcggcatg ccaactggga
    16381 agacaaaacc attacgctta tccacttcac gattcccccc tacagtagca tctgccgtca
    16441 agacaatcgc tttataacct tcatccttca cacggtccat gatgtggcgg ttgataccgt
    16501 catccttact aaagtaaaat tgaaaccaat gaggtgtccc ttgaagggct tcagaaatct
    16561 ctggaaggtc aacagtagag taagaactgg ttgtataaag agaaccaaac tcatgcacac
    16621 cacgcgcagt cgccacttcc ccctgttcat ttgccaattt atgagccgca acaggtgcca
    16681 taatgattgg cgaagatagt ttttcacctg caaattcaat ctctgtactt ggattttcaa
    16741 catcgcaaag tgtatgagga acgatgagtt tgtggttaaa ggcgcggata ttctctctta
    16801 aagtgaaagt atcttccgcc ccactagcga tatagccaaa tgctgcttta ggaataactt
    16861 gttgcgccat tggctccaaa tcataggtat tgatgaagtc tacatgacct tctgcattgc
    16921 ttgttttgta tgacataaac tgtcctcctc gattaagtaa gcgtttgctt tttactatac
    16981 aactataata actctttttt caatcatttt caagagtttt gcttaaaaac catcttattt
    17041 tttcatacaa tatactagcc ctactttcct taaactctaa accttagtct atattaagtg
    17101 aatcgttgag attttttgct gccaggcatt tgaactactt ttttatttgc tgattctatc
    17161 tactaatact ataaaaaaca agtgaataag acaccagaac atcctctaat aggacctatg
    17221 gacttatatc agtcccatat atactgttta agcaagatac tatcctagtt gacgctaact
    17281 tcctatccca cttattaatt ttttatttat agtgaatctg aacaaatcaa ttggggaatc
    17341 caagtcaatt tctagtactt tttgaaaagt ttcagcgtac tatcgtaaaa tgaaataaaa
    17401 catgcacaaa ttgattaggg aatttaaacc aatttctaac aatgtcttag aaatcaaagt
    17461 gtactatttc aatttcaata cactacactt cattttctcc ttaaattcag tctctttttg
    17521 aataaaaaaa tcctctggta agagccagag gataagaatt tatttcattg ttgggaagag
    17581 caatacatca cggatagttg ttgtatcagt gaggagcatg cagagacggt cgataccgat
    17641 tcccaaacca cctgttggtg gcataccgta ttcaagagct tcaatgtagt catagtcgat
    17701 tcctgtcgct tcatcatcac caagttcttt ggctttagct tgggcttcaa aacggctaag
    17761 ttggtcgatt gggtcgttga gctcagtaaa ggcattaccg tactccttag tcatgataaa
    17821 gagctcgaaa cggtcagtaa agcgttggtc ttcaggattt ttcttagcga gtggagatac
    17881 agctactgga tgtccataga caaaggttgg ttggattaaa gtttcttcaa caaactcttc
    17941 aaagaaggca ttgatgatgt gaccaacctc agtgtagtgt ttctcaactg gaactttctt
    18001 ctcagcagcg atagctttag cttcttccaa agtcatgtct tgccagaaat cgacaccagt
    18061 aatttctctg atagcatcca ccatatgaac acgcttaaat ggttcgttaa tcttgatttc
    18121 agtaccttga tagttgactg ggccatcgcc cttaactgat ttagcagcat gttggataat
    18181 gccttccgtc aagtccatga tatcttggaa gtctgcataa gcttggtaaa cttcgataga
    18241 agtgaactca gggttatgag tagcatccat tccttcgtta cggaagatac gcccaatttc
    18301 atagacacgt tccataccac ccacgataag gcgttttaag tgaagctcag tcgcgatacg
    18361 aagcaccatg tcaatgtttt gggcattgtg gtgggtgata aatggacggg cagcagcacc
    18421 accagcttcg ttgtgaagaa caggtgtttc cacttcaagg aatccttttt ggtcaaggta
    18481 acgacggatt tcagagatga tttttgaacg agtgacaaaa cgttcaaagc tttcacgatt
    18541 agaaatcaag tcaaggtaac gtttacggta aattgtttca acgtctgtca aaccatggaa
    18601 tttctcagga agaggacgaa gagccttaga caagtgtgtg atgtgggttg ccttgataga
    18661 gagttctccc atatccgtac gcatcacttc accttcgaca ccaaggaagt caccaagatc
    18721 tgcttttttg aagatttcgt agttttcttc accgacagca tccttacgaa cgtagatctg
    18781 aatctggcct tcgcggtctt gaaggtgggc aaaaccaact tttcctttac cacgtttggt
    18841 tatcaagcgt cctgcgatag tagctgtttc gtttttatcg tgtaattgtt ctttatcgag
    18901 gttggcatat ttatctttta attcttgtga atttgcagta cgttcaaaac gttttccgaa
    18961 aggatcgatt ccttgttcgc ggagcgcagc cattttttca cggcgaacga tctgctggtc
    19021 atttagttct tccatatgtt ctgtagacat gggatcctcc tagttttact caaaattgaa
    19081 tacgatgagt cattttttgc gatactccca ttttatcata aataagcaag aaattcacgg
    19141 ataatcttta aaaactaaaa agaacctgac gctaaaatca ctggaaagaa gtgaaagtat
    19201 ctcccgcccc actagcgata tagccaaatg ctgctttagg aataacttgt tgcgccattg
    19261 gctccaaatc ataggtattg atgaaatcta catgaccttc tgcattgctt gttttgtatg
    19321 acataaaatg tcctccttaa taagtaagcg tttactttgt atattataaa aatatcttaa
    19381 ctcttttttc aaaacttttc aaatattttg tttggaaatt tcagaaattt tatgtctatg
    19441 gtaaaaaatc cttataacgg caataaaaaa tagatattat ccaaagaaga ttttaagtgc
    19501 tacaataact gtattatttc tagatgggag gttctatttt tggattggtc cattgttgaa
    19561 caatatctac cactatatca aaaggcattc tttctgacct tgcatattgc agtttgggga
    19621 attttgggat cctttctgct cggtttaatc gttagtatca tccgacatta tcgaatccct
    19681 gttttggcgc aagtagcgac agcctacatt gaattgtcac gtaatacgcc ccttttgatt
    19741 caactcttct ttctctactt cggtcttccc cgaatcggca ttgtcctatc ttcagaagtc
    19801 tgtgcaacgc ttgggcttgt ctttttagga ggctcctata tggcagaatc tttccgaagt
    19861 gggctggaaa ccatcagtca aacccagcag gagattggcc tcgctattgg tctgacacct
    19921 ctacaggtct ttcgctatgt ggttcttccg caagcaacag cggtggcact cccctccttt
    19981 agtgccaatg tcattttcct tatcaaggaa acctctgttt tctcagcagt ggctttggcc
    20041 gacctcatgt acgtcgccaa ggatttgatt ggtctctact atgagacaga cattgcgcta
    20101 gctatgttgg tagttgctta tctgatcatg ctgctaccca tctcactggt ctttagctgg
    20161 atagaaagaa ggacccgcca tgcaggattc gggaatccaa gtactctttc aaggaaataa
    20221 tctcctgaga atcttacagg ggttgggcgt tacgattggg atatccatcc tgtctgtcct
    20281 cttatccatg atgttcggaa cagtcatggg aatcatcatg acctcccatt ctagaatcat
    20341 acgattttta acacgattgt atctggactt tatccgtatc atgccccagc tggtgctact
    20401 cttcatcgtt tactttggct tggctcgaaa ctttaatatc aatatctcag gtgagacttc
    20461 agctattatc atttttaccc tctggggaac agctgaaatg ggagacttgg tacgtggagc
    20521 tatcacttct ctccctaaac atcagtttga aagtggatag gcactcggct tgactaatgt
    20581 tcaactttac taccacatca tcatcccaca agtcttaaga agactgctac cgcaggctat
    20641 caatcttgtc actcggatga ttaaaaccac ttcattagtt gttttgattg gggttgtgga
    20701 agtgaccaaa gttggacaac aaatcatcga tagcaatcgc ctgaccatcc caactgcttc
    20761 attttggatt tatggaacca ttctagtctt atatttcgca gtttgctacc ctatttccaa
    20821 actatccact cacttagaaa aacattggag aaactaaatg tctgaaacta tcttagaaat
    20881 caaggaacta aaaaaatcct tcggagacaa tcccatcctc caaggacttt ctctagaaat
    20941 caaaaaaggg aagttgttgt catcctaggg ccatctggtt gtgggaaaag taccctcctt
    21001 cgttgcctca acggcttaga aagtattcaa ggtggagata ttcttctgga tggtcagtct
    21061 atcgttgaaa ataaaaaaga ttttcaccta gttcgccaaa agattggcat ggtctttcaa
    21121 agttatgaac tctttcccca tctggatgtc ttacaaaacc tcatcctagg ccctatcaaa
    21181 gctcaaggaa gggacaagaa agaagtaacg gaagaagctt tgcaattact agagcgtgtc
    21241 ggtttgctgg ataaacaaca tagctttgcc cgtcaattat ctggtggaca gaagcaacgt
    21301 gttgcaattg tccgtgccct cctaatgcat ccagaaatca tcctttttga cgaggtgact
    21361 gcttcgctgg atccagaaat ggtgcgtgag gtgctggaac ttatcaatga tttggcccaa
    21421 gaaggccgta ccatgatttt agtaacccac gaaatgcagt ttgcccaagc cattactgac
    21481 cggattatct tcctcgacca agggaaaatc gctgaagaag gaacagctca agccttcttt
    21541 accaatctgc aaaccaaacg agcccaggaa tttttaaacg tctttgactt tagccaattc
    21601 ggctcatatc tataaaggag attcttatga aactattcaa accactctta actgttttag
    21661 cacttgcctt tgcccttatc tttatcactg cttgtagctc aggtggaaac gctggttcat
    21721 cctctggaaa aacaactgcc aaagctcgca ctatcgatga aatcaaaaaa agcggtgaac
    21781 tgcgaatcgc cgtgtttgga gataaaaaac cgtttggcta cgttgacaat gatggttctt
    21841 accaaggcta cgctacgata ttgaactagg gaaccaacta gctcaagacc ttggtgtcaa
    21901 ggttaaatac atttcagtcg atgccgccaa ccgtgcggaa tacttgattt caaacaaggt
    21961 agatattact cttgctaact ttacagtaac tgacgaacgt aagaaacaag ttgattttgc
    22021 ccttccatat atgaaagttt ctctgggtgt cgtatcacct aagactggtc tcattacaga
    22081 cgtcaaacaa cttgaaggta aaaccttaat tgtcacaaaa ggaacgactg ctgagactta
    22141 ttttgaaaag aatcatccag aaatcaaact ccaaaaatac gaccaataca gtgactctta
    22201 ccaagctctt cttgacggac gtggagatgc cttttcaact gacaatacgg aagttctagc
    22261 ttgggcgctt gaaaataaag gatttgaagt aggaattact tccctcggtg atcccgatac
    22321 cattgcggca gcagttcaaa aagacaacca agaattgcta gacttcatca ataaagatat
    22381 tgaaaaatta ggcaaggaaa acttcttcca caaggcctat gaaaaaacac ttcacccaac
    22441 ctacggtgac gctgctaaag cagatgacct ggttgttgaa ggtggccatt aactcttaaa
    22501 agggactgga ttttaagctc caatcccttt ttaagatttt acctataaca tcctgagtct
    22561 atctaagatg ttcaatctga acacagtgta catactttat cttctattgc atatacttta
    22621 tcacataaga tacgaatatc ctcttcacta tgactagcaa tcaaaattgt tgtccctttt
    22681 tcactagaga gctttctaaa caatgttctc atattttcta cacttgattt atccaaggca
    22741 ttcataggtt catctagtaa aagaatagag ggattctcca taattgcttg agcaatccct
    22801 agctttttcc tcatacctag cgaataagtt ttaactttct ggtctttttg ctcatataga
    22861 ccaactattt tcagtgtatc attgatttcc tgattaccaa ctactcctcg tatgcttgcc
    22921 aaatattgta aattcttaaa gccactataa taatttataa aaccaggttc ttcaatcaaa
    22981 gctcccaaat tagctggaat ttttctctca ggaacaatat tttccccatt gattaacact
    23041 tctccataag acggactata taaaccagct attaatttaa acaatacact tttccctgag
    23101 ccattcgcac cagtaattcc tataatttcc ccctgtttac aactaaagtt aaggttttga
    23161 aaaacacatg tcttttttaa tttcaactca atatttttta atatgattat ttcattcatt
    23221 ctataaacct cctcttttga cgagtgaaat agaaaatgct ttgaaaaaga aagactaaaa
    23281 atagcaactg aagaaataaa tctcgtccta tatctccatt ccctcgattc aaaatataaa
    23341 atagataatt agttcgattt cctacaaata gaccaccaaa cacaatcatg agtaaaaaga
    23401 aactaacgca agcaaagttc atttgtacat tcgctcctag tgcgtataac ataaagcaaa
    23461 acaagactaa cacacaagca ttcaaagaat caactataaa caaaactcct atatcccagt
    23521 ttggtaactg taacataaat tgaatcacta gaacttttac catgattaaa atgagtccat
    23581 aaagaaaaca ggtccaaatc tgatacctaa tccagagctt aatagaagaa aatcgaatca
    23641 cttccataaa gaaatcggat gctcttctac gatatacaac tgctagaaaa aatgtatgaa
    23701 taataaacca atgaagcaac catatagata agactaaaaa agagttttga ctcagattag
    23761 gcccttttaa aatttctata acaagtctat catttccttc taaccaatac cataatccta
    23821 aacaaatcaa tatccaaaaa agaacataac cccaaaagta cttggtatag attatctgat
    23881 tcaacctcca catgtgacta cttaactttc ccatttccaa cttttacact ctcctccagt
    23941 ttctatgaac agaaaataca gtcaatggaa ttatgataga tagtaatgta agccaataaa
    24001 tcaatgcata cggattggaa tttaccatat actgaaaaat tggcaataat ccaattatcc
    24061 cattgtccaa ctgataaatc cagaataagg attctacaaa taaaaaagta aacagaaata
    24121 ctaagctcat gtttgatttt ttaaaccaag aaaaactgct aatagtaagt aaggataaaa
    24181 agaaatagta tgctatataa gagaaaaaaa atcctataaa gaaactagca ttgtttgcaa
    24241 tacttatacc ataaaattct cttaaaaaat caacctcctt tatctccaaa gagaagctaa
    24301 aaccattact aaatgcaatt agaaaaatca ataaaaataa agtcgccgtc caaatccccg
    24361 tactaagagc tgctaatttg aaactaaaac tggtaaagtg cttaattgat ttcagacgaa
    24421 tgcgacactc caacctatta aaatagttat tcatcaaata aaaaaagaac aatatatatg
    24481 tgaacggaaa gcaatatact ccagtcgtca tatcttgaag taaaactaag atccattcta
    24541 atacatttgg atggattgaa tattggcgac agcgcaataa atatactgta ctagataaaa
    24601 cacaggatag cagtaatata aataaaccaa tactgataaa aaatcttttt tgtaaaattg
    24661 aacaaattgt ttcattatac gtagtcctct gaatgtagaa aaaatgtacc ataaacaacc
    24721 aaacaactaa caaataaaat aaaagcaaga tgcccactaa ctaaggaaag actgatatct
    24781 ttctgatatc ccaaagctaa tgttgtcaca ggttctaagt aagatagccc taaaatagcc
    24841 caaaaaatac caccaaccat catataggca actgggatga aaatagctcc tatttttttc
    24901 ttcactagca aagcactagc tagtccaaaa atagagaaca cagtgcccca aactccatac
    24961 cagagagtcg ttacaagact atagagaaac tgattggaat caaataattc ttttaaggca
    25021 ccactataat ctccaatata aatttcctga taaggagtca ctaaaagatt aattcctaat
    25081 aataataaat aggggagaaa aaagactaga aaagaagaaa taattacagt actacccaca
    25141 atagccagat acttcttttt agaaattcga cacaattgtg ctgttagaaa atgactctca
    25201 gcatcctcta ttatctgact agaatagggc agtgtacaga taagtgcagc tactaggcta
    25261 atcggtgaaa atcctcgaat agaagaagcg gcaaaaaaaa tcgataaacc ttcaatttta
    25321 taaataccat tgaaagcaag gaaatttcct aaactcatgc aaagaagggt caaaaataaa
    25381 gacatataaa atcgaggtga ttgaacgact cgtacaagat tacaaatgaa aaattccatc
    25441 cttactcctc cttataataa aaatagggtg tagcattctt ttttcatgct acacccacaa
    25501 tcaaccatct ctaaggctta ctctgacaag taagttaata agaatctgga ttccaagaac
    25561 ctgaagtatg aattcttaca tgatttccaa attgtggcgc catagctaat ctagtaccag
    25621 aaccaatata attgtcacca cctccattat agtacatgac aatcctagag ccagacccca
    25681 atgaatatac cggggtaata tctgacccac tataggcgct acgaatagag gtatttaacc
    25741 ttttaccgcc accagtgctg tcattattat taattccagc agaggcgttt tctttctcaa
    25801 gctcactagt tgtatccgaa ccccaacgcg gcgacgttaa atcataatct ccaatctgtc
    25861 tagctaaaac tgtagggaga aacaaactag atgcaattgt aactacgcaa aaaaggaata
    25921 tatttttctt cttttcatga catcgtcctc ttaagaagtg ataaacaaga tagttgattg
    25981 atcaatttga tacatattac attttcatta tatcaataaa agaaagcgtt gtcaattatt
    26041 ttatttaaaa tattgtcata ttttaaataa aatggtaaaa gagactcggt agcctcgtct
    26101 ctctttatct tactgaccta accttcttgc caattttctt ggtcttcttt aaagcgtttt
    26161 agaagatcaa ggccctctgg cgtgatgtaa ccttcttctt gggctagatg gataagctcg
    26221 ctatagtttg aaagcgtcac aagtttaaca ccagcatctg taaagttctt atctgctttt
    26281 ggcaattggt agctgaaaat cgctacaact ccaagtacat ctgctccttc tcgcttggct
    26341 gctgctacag cttcaagaac tgaaccaccc gttgaaataa ggtcttcaac cactaccatt
    26401 ttttgacctt gagctacgcg accttcgatt tgattaccag ctccgtggtc ttttggtttg
    26461 ctacggatgt aggcaaaagg caagtccatc ttatcagcaa taatggctcc gtgtggaatc
    26521 cctgctgttg cagttcctgc aatcacttct acttcaggaa aggcttcttt gatagcttcc
    26581 acaaaaccat tttcaattag ggtacgagtt tctggatagg ctagtgtcac acgattatca
    26641 gtgtaaatcg gtgacttgat accagatgcc caagtgaagg gttcctctgg tttgaggtaa
    26701 acggcttgga ttttcaagag gtggctagcg atatctttag caagtgtcat ggtattctcc
    26761 ttttattttt ctaatctagt tctttaattc cagtcctgtg tccattcatc cttgatggca
    26821 tgataagctg caacaggatc ctcagcttgg gtaatgggac gtcctactac gatatagtca
    26881 ctgccgattt gataggcatc agcaggtgtc atgactcgtt tttgatctcc aactgcaaca
    26941 ccagctggac gaatccctgg tgtcagacag ataaaatctg gattggtagc ctgcttgatg
    27001 acttgtactt cctgagccga gcaaacaaca ccatccaagc cagcttcagc tgtcttcttg
    27061 gcatagtgaa tcacagactc ttgcagactg gtttggatat tttgaaactc ctgcatctga
    27121 gcttctgacg ttgatgtgag ctgagttaca gcgatcaatt tagcttgact tccaagacct
    27181 tcacgcgcag ccttcatcat ctctacacca ccagccgcat gaacattggt catatccaca
    27241 ccaagctgag ataggatctt catggctgac ttgactgtat taggaatgtc atgaagtttg
    27301 agatccaaaa agacactatg acccaaacct tttaagtagg acacaatctc aggccccgct
    27361 gcgtaataaa gctccatccc taccttgaga taaaggcttt cttctgctgg gaaaagagct
    27421 aaaaattcct tgaccgcctc aaaactagga aaatcaagag caatgattga acgatgttct
    27481 cgcataggtt tctcctttta cctttactag tgagagagtc tggccaacag aagccacgaa
    27541 aaaaggaacc tgccaacagc aaggttccac gaaaaatggg tagtctccac cctttcaccg
    27601 tctaacctta gctgcctctc tggactgctt taaaggtttt ctattattct attatttcta
    27661 ctagcacttg tcaagtaaaa acatggtcac taaagaacta taagagaaaa ggtaaaccta
    27721 gcgacgcgat gaacgctggg tcgtttggtt tcgattgctc tcttcctctt gttttttctg
    27781 ttcttcttct tgttttttct cagcttcctt ggcctcttgt ttggcttttt cctcagcttc
    27841 cataattaat ttatccgcca cagtgtaact gtaaattcca gcttccatgt caatcacact
    27901 cggttctgac aattgtggct taatcttact gtaatatggc agtttcttac tcatttcaga
    27961 tagaggaacc aagacttcgt ccgaatcatt catggtcaat cgaattaaat cggatgtcac
    28021 cttgcttggg gctaattcca ccttttggat agccgccttg agttctgggc taatttgagc
    28081 aagttctgag acaaaaacct tgatttgttc actatcatta aagagaactg ataaataagt
    28141 ttctggtaaa ctgttcagac tcacagaact agtctcaagc tgaccactgg aaagaatagg
    28201 ataatgattt tcaccagaaa tatagtaggc cacaatatca tattccttga ccttaatagt
    28261 gaacttagtt ggaaattgat agacaagttg agctgattca acccaatagt tagacttaat
    28321 ctgcttttca tattttgcct tgtctagcag aaggttaatc gtataatccg aatcctgaat
    28381 gcctgaagcc tgtcgaatat catcagctgt agtttgcacc gttccctcaa cacgaatatc
    28441 tttcatggtc gcataaggac tgagcaagta ggcagagaca atcaataaaa gcagacttgg
    28501 aaataaaatc gtgaaggctc gcaagatatg gataccagga atctttgctt tggctggttt
    28561 ttcctttata gcctttttag caagcttttt atcctgttcc tccttctctt tagactctga
    28621 ttcttctatc tcttcttttt ctttgtcagc ctctgaggat gccacttttt cttcagactc
    28681 ttccttagct gattctgaat cttcctggtc tgtttcactc tcctggtcct gtttatcctc
    28741 tgacttctca gattcttctc ccattcgagc ttgtctttcc ttttccttct cctcagctag
    28801 agccgcctct tcttcagcct tcttttttag atattcttgg tttcgtttct gccattctga
    28861 taactctttc aattcttcga gggtttcttt gtcctcattt ttcttatctt ttgacattta
    28921 ctttccttat gataaatctt ttttcaacaa ttgataaaaa tctgctagag atttcaattc
    28981 cttagaagct ttcatcttag cttggtaatc ttccttgtga cttagtaagt gagaaagctt
    29041 ctcttccaaa ctatccaagg tcaaatcgct ttcttgaagg tcttctgcat agcctttttt
    29101 tacaaagtaa gctgcatttt caatctggtc accacgacta gcttcacgac caagcggcac
    29161 aatgacatgc aattttgcta tcgccaagag ctcaaaaatc gtattggcac cacctcgtgt
    29221 cacaacaata tcagccaatt ccatcaaggg ttgatagaga tcggtcacat agtcaacacg
    29281 aaaaagattt tgcctcaact cattcagact agaatctcca gttagattga taatattgta
    29341 gcgctctgtt agttctttct tatggtctgt caccaattgg ttaaagacac gagcgcctgc
    29401 agaaccgccg acaaacaata cagttggcaa tttgggatta aagtgggttt gaatatccac
    29461 caattcatct ggttctggag tgtttttgtc cgaaaccttg gtcaccgctc ccacatgctc
    29521 gaccttagcc aaactcgaag cttgttcaaa ggttgaatac atcttagtcg caaatttata
    29581 ggcgatttta ttggccaagc ccatagacag gtcagattcg tgaataaaga caggcactcc
    29641 tgacacacgc gcagcgataa caggcggtac tgagacaaag cccccctttg aaaaaagggt
    29701 ctgtggacgc agtcgcaaca tgataaagag cgattggacg attccccagc caactttgaa
    29761 gacgtccagc atattttgcc aagagaaata gcgacgcaat ttcccagtcg caatagaatg
    29821 gaaggtgaca tccaaacctg acttaaggat ttcttggtgt tcgataccac gcttgtcccc
    29881 gatatagtgg acttcccaac catcttcgat gaacttgggc attaacaaaa gattgagggt
    29941 aacgtgtcca accgtccccc cacctgtaaa gacaattttt ttcatatttt attcttttaa
    30001 ctccgctact gtgtcgataa agaggtcgcc acgtacttca aagttagcat acatatccca
    30061 gctggcattg gcaggactaa gaagaaccac atctccttga gtcgcaagtt cataggcctt
    30121 acgggtcgca tctgcaatat ctgtcgcctc cacataagcg acaccagcct tgtctgctgc
    30181 ccgtttgaca cgttctgcag attgacccag gatgaccatc ttcttgagtc cagtaatgtc
    30241 tggcaccaat tcgtcaaact cattgccacg gtccaaacca cctgcaatca agacgacctt
    30301 gctgttgtca aatcctgata aggctttttg agtagccaag atattagttg atttactgtc
    30361 gttatagaat ttaacaccct tgatgtcatc cacaaactgg agacggtgtt tgacaccacc
    30421 gaaggctgaa agagtttcct tgatggtttg attgtccaca tcacgaagct tggctacagc
    30481 aatagtcgca agggcatttt ccacattgtg gctacctgga acaccgattt cattcgctgc
    30541 catgactact tcaccacgga agtagagttg accatcttcc agataagctc catcaacctt
    30601 ttcaagtgtt gaaaatggta caacagtggc ttctgtcttg gaagtcaagt cttttgccaa
    30661 gtcttgatta aagttcaaga caaggaaatc agctgctgtc atcttgttct ggatattcca
    30721 cttggctgct acatattccg aaaatgaccc atggtagtcg atatgagttg gcatgaggtt
    30781 ggtaataacc gcaatctctg gatggaattc ttgaacaccc atgagttgga aagaagaaag
    30841 ttccataaca agcgtgtcct tatctgatgc tatttgagca acctgactag ctggatagcc
    30901 gatattccct gataaaagac catgttgccc agcagcagtc aaaacttccc caatcatagt
    30961 cgttgtggtt gtcttaccgt tcgatcctgt gataccaata atcggtgctt ctgaaatcaa
    31021 ataagccaat tccacctcag tcaagactgg aattcccttg gccaaagcct tttcaatcat
    31081 gggattgttg taggggatac ctggattttt caccataagg gcaaactctt catccaagag
    31141 ttccaaagga tggccacctg taatgacctt gatcccttct tccagcaaac tttgggcagc
    31201 tggattgtcc tcgaaaggtt tcccatcatt tactgtcaca atggcaccta gcttgtccaa
    31261 caaacgagct gcagattcac cagacttggc caagcctaaa acaaggactt tcttattttt
    31321 aaattgatct attactttca tgtctcgaac tccatttcta ctcctactat tttaccattt
    31381 ttatggaaat aaaaaagcca caaagtgtgt ttgtgactct ttcttctaac tgaatcttac
    31441 catatcatct atgtgataaa tcggtaactc gaatgacctg atccacttgc tcccaaatca
    31501 gaggattatg ggtcgcaata ataatggtcc gattcggatt ttttaaagat tctaggatgg
    31561 aaagtaattc ctcagagttt ttggggtcta aggaagcggt tggttcatct gcgaggatca
    31621 aaggtggatc ctttaaaatt atcttcgcta gtgcaacacg ttgtgcttct cctcctgata
    31681 actcaaatat aggttgcttc aaatccaaat aagagaggtt tacacggttt agagcttgtt
    31741 tcatcaaaga gattttctct ttttccttca actttttacc aactaaaccc agattgagat
    31801 tctctttgac ggtttggctt tcaattaagc caaaatcttg aaataagtat cctaagtaat
    31861 ctctaaagaa aacagaaggc ttaatgtcct taagagaagt gccatcatat atgatttgcc
    31921 ctttgtcata tggctctaat cgtccaatca tattcaagag tgttgtctta ccacagccac
    31981 ttgtaccgat taaggcataa attttcccac cttcaaaatg aagattcgta tctgaaaata
    32041 gctgacggct tccaaatttt ttagatatat tctttagttc aatcatccta ttttcctttc
    32101 ataattgtca tagaaacacg agattctttc tgcgcttgac ggtaaagcgt caaaactgca
    32161 ctaactagaa agaccaataa agtgagcaag ccaatcacca agtctcgact gcttaaaata
    32221 aagagactag caccaaatac aaaactggca aattggctaa ccatatactg agcatgtgtt
    32281 tcaaaaaatc gtaaacctga aattcgttta atcaagatat ctcggcggaa ttgctcgaaa
    32341 tatagaagat tgacagaata aaagagtaac aaggaactgg ctattccaac aatagctcct
    32401 aagattaaag ttgctgtttc agtttgaact tcattataac gagttagata aacacttctt
    32461 ccttctttaa gataggatac ttgctcataa attccagctt tcttcaagag ttctagccca
    32521 ctctcatatc ctttgataaa gagttgtttt ccagcattga tagaccaact agataaggat
    32581 ataaaactat cacctgtaga agtcggcgtg aatacaacta aaatcggatc agtcaaatac
    32641 tgagtagata cgggattctc accgttatta taaacaaacc gcttttctcc cattgaaaga
    32701 taactaacgt gcgctttcat ctcataatcc aaaggagcac ttgcctcctc accagatttt
    32761 ccataataac tcaatctttc ttcaaaaact ttcttaagtt ctgcttctcg agagcgcaaa
    32821 tgttctggga gcaagaggat aaactcacct ttttggagat gggctaactt ctgtttggtc
    32881 tcagcatcta ccacgacctt ttccttgtcc aaataactgg gactaacata gagcgtatta
    32941 gcatctggac tataggtatc cagtgtctct ccctgttcat tttttccttg tggattggca
    33001 aaatggagca gattatcctt tacataaaga gcttgttctt cttcgattgc ttccttggca
    33061 aaggcatacc acttgctctg attttctgta tcttttcctc tatcacctaa gccaaaggaa
    33121 atctggtaat agtctgctct gtcctgccat gcttgttttg aaatttcaag ttctttcaat
    33181 cgttggtaag acgtcaaacc tgtcttaaca gcgtagccta ctgtaaaaac agctactaac
    33241 tgacacaata gggttaaagc catcaagcgt ttaaggggta atcttccctt aataacggga
    33301 actaatgctt tgtaactcaa actcattagg taaaggagca ttagtaaaat tgaaatcgcc
    33361 aataaaaaca acagatagaa actaatccca aaaccatagg tggctaacaa gataggataa
    33421 aacaaacctt gactaaaaag aacgactccc ccacctagga aggaaaggag ggctgataga
    33481 aggagccact tgatatcagt agataaaccc caattattca cccctaatct aaaaaccatc
    33541 cagaatcctt gccttagctt agatcctgga tggtttcttt tttcacccaa tgggtgtttt
    33601 ttactagaca aaaaagagtt tcccctttat ggtataagtg tagaaaaaaa cacaaaaaga
    33661 aaggaaactc atatgaacag tttaccaaat catcacttcc aaaacaagtc tttttaccaa
    33721 ctatctttcg atggaggtca tttaacccag tatggtggtc ttatcttttt tcaggaactt
    33781 ttttcccagt tgaaactaaa agagcggatt tctaagtatt tagtaacgaa tgaccaacgc
    33841 cgctactgtc gttattcgga ttcagatatc cttgtccagt tcctctttca actgttaaca
    33901 ggctatggaa cggactatgc ttgtaaagaa ttgtcagctg atgtctactt tccaaaattg
    33961 ttggaaggag ggcagcttgc ttcacagcca accttatccc gttttctttc cagaactgac
    34021 gaggaaacag tccatagttt gcgatgcctc aaccttgaat tggtcgaatt ctttttacag
    34081 tttcaccagc taaaccaact cattgtagat atcgattcta cccatttcac aacttatggc
    34141 aagcaagaag gtgttgctta taacgcccac tatcgtgctc atggctatca tcctctttat
    34201 gctttcgagg ggaagacagg ttattgtttc aatgcccagc ttcgtcctgg taatcgttat
    34261 tgttctgaag aggcagacag ctttatcaca cctgttttag aacggtttaa tcaacttctc
    34321 tttcgaatgg atagtggctt tgcgacccca aaattatacg atttaattga aaaaacaggg
    34381 caatactacc tcataaaact caagaaaaat actgttctga gccgtcttgg agacctttcc
    34441 ctcccttgcc cacaggatga gaacttaacc atcttgcccc actccgccta ctcagaaact
    34501 ctctatcaag caggatcttg gtcgcacaag cgtcgtgtct gccagttctc tgaacgaaaa
    34561 gaaggaaact tgttctacga tgttatttct ctcgttacaa atatgacgag tggaacaagc
    34621 caagaccagt ttcagcttta tcgtggacgt ggtcaagccg agaatttcat caaggagatg
    34681 aaggagggat tttttggcga taaaacggat agttcaacct taatcaaaaa cgaagttcgt
    34741 atgatgatga gctgtatcgc ctacaatctc tatctttttt tcaaacatct agctggaggt
    34801 gacttccaaa ctttaacaat caaacgcttc cgccatcttt ttcttcacgt ggtgggaaaa
    34861 tgtgttcgaa caggacgcaa gcagctcctc aaattgtcta gtctctatgc ctattccgaa
    34921 ttgttttcag cactttattc taggattaga aaagtcaacc tgaatcttcc tgttccttat
    34981 gaaccacctg gaagaaaagc gtcgttaatg atgcattaaa gaacagtcga gatgaaaaaa
    35041 tcgtgtgacg caccaaggga ggagtctgcc cttttgagga aatctagcga ggaaaaacga
    35101 tactggaaca gcagaaagta aaactgacct catgaggagg aagaaagtgg ctcatgaggt
    35161 caggggtttt gtaagttaca tctagttgag agaggtatga atgatttggg ataaagaatc
    35221 ccccatgatg gataagagag tctgaccaga aaagagtttt atacctgctg ctctcatttc
    35281 cttaatccga gtgataatca ctaaagcaaa gaaagataag ccaaatattg ctaaactaat
    35341 taaaataagg ggatttagta atattcgaaa agcaagaaaa tagggcggta tctttcggtc
    35401 agcacttgct ttataaccca aatctcctaa tttatcggca agcttttctt tcgtcaagga
    35461 gcctgacaaa aggagataac tatttagcgg atcactacgt tcacgacttt cttggctagc
    35521 ttcttggaat tcttttggta aagttccctg accataagtt gcataagtaa agtgagtcgt
    35581 cccatcctta ctcggctcta caattcttct agctattaaa ctctgttctg agtttgcaaa
    35641 attctccaat tcctgttcaa atacctcacg cgtcggttcc tgagtatctt ttttgacacg
    35701 aagtaaagaa acggaatcat agcttgcata taaatattgt ggcgcacgta agacaataat
    35761 ccaagcaagg aagaagctga gaaaaaaagt tgataataat atgaatagtt tcttcatagt
    35821 agactccttg taaacaaaat tccccctgta atttcttaca aggggaacga tttaaatcaa
    35881 tgaacgatta gtcataatca cagtaaaatg ctacttgttc tccccattta gtccaaatcc
    35941 atgcatagga ggtttttcca gcaggagctt ctcctttatc agaatgacca tcccatctgc
    36001 tcacgatgga tgaataatga tattttttac catgatagta atttgaaaaa gcctaaccac
    36061 ctcctgaacc ttctccatat gtccatactc ctccatctgg atattataca gcagctgatg
    36121 cagctcccaa taatgtaaaa cttgaaataa gagctagagc aagtaatcta tgttttttcg
    36181 ttttcatttt attttttctt tcaaaaaaat cacaccttga gcaacaatgc aacaaaataa
    36241 atcctcctct ctcttttatt gaaaccgctt tcttgtgtga taagaataac atttttatta
    36301 tttgttgtca aggaaaaaat cgaatttttt agatgtttta ctatattatc tctatgaata
    36361 atattatata gtagttttat ttcaaaataa tatgcaacca gtactaacca aatataaaat
    36421 agatgccatt aacgaatttt attcaagttt ttcccattca tactatacaa gcaaaagaga
    36481 tggtgttaac taaaaagcaa ttcaaactat tataaaattc ctagcaaaaa gagagccgaa
    36541 actctctttt ttatcttctt ttactttttt tgactggcat gagtgtgatg tctctaacac
    36601 taaagtaagc taggatcaac atggctattg ctaggaatat ttctgttggt aattgaaaaa
    36661 ttttcagaaa agatagaacc aataaaatca agagtgccac taaaatacat accatagcga
    36721 cgatattgac tgtcccttta atgctttctg gtgtcgcaaa tacatagagt aggagcagta
    36781 aaattcctag gactaaatag accatctttc tctctttcta gctcttattc agctgatttt
    36841 ttcttcttat tagctttctc acgctctgct ttgttaagga tttgtttacg caaacggata
    36901 gactcaggcg ttacttccat gtactcatcg tcgttcaaga actcaagaga ctcttcaagt
    36961 gtcaagatac gaggcgtctt gataacagct gtttggtcct tagtagctga acgaacgttg
    37021 gtcatttgtt ttgccttcgt gatgttaact gtcaagtcat tttcacgaga gttttcgccg
    37081 atgatcattc cttcataaac ctcagtacct gggttgacaa agatcgtacc acgttcttcg
    37141 atagacatga ttgagtaagt tgtagcctta ccagcatcga tagaaacaag ggcaccacgg
    37201 tgacgtccac caatttcccc tggaatcaat ggcaagtatt ggtcgaaggt atggttcatg
    37261 ataccgtaac cacgagtcat tgacaagaac tcagttgagt atccaatcaa accacgcgct
    37321 ggaacaagga agaccaaacg agtttgacca ttaccagttg aaatcatatc caacatttca
    37381 cctttacgtt cagaaaggct ttggataaca gacccttggt attcttctgg agtgtcgatt
    37441 tgtacacgtt caaatggttc acatttaaca ccgtcgattt cttttacgat aacttctgga
    37501 cgagatactt gaagttcata gccctcacga cgcattgttt cgataaggat tgacaagtgc
    37561 aattctccac gtcctgaaac agtccattta tctggtgaat cagttgggtc aacacgaagg
    37621 gaaacgtctg tttgcaattc tgcctgcaag cgttcttcca ccttacgaga agttacccat
    37681 ttaccttctt taccagcaaa tggtgagttg ttgaccaaga aagtcatttg aagagttggc
    37741 tcatcgatgt gtaggattgg aagagcttct actgcatctg tcggagtgat ggtttcaccg
    37801 acaaagatgt cttccatacc tgaaacggca atcaagtcac ccgctttggc ttcttggatt
    37861 tcacgacgtt ccaaaccaaa gaaaccgaag agttttgtaa cacggaagtt tttagttgta
    37921 ccgtcaagtt tagaaagggt aacttggtcc ccaaccttaa ctgtaccacg gaagacacga
    37981 ccgataccga tacgtccaac gaagtcattg tagtccaaaa gtgacacttg gaactgcaaa
    38041 ggctcatctg agttatctac tggagctggg atatggtcga taatcgtgtc aaagattggt
    38101 gccatagtcg cttcttggtc agctggatca tctgacaatg aagaagttcc gttgatcgct
    38161 gaagcataaa ccactgggaa atcaagctgg tcgtcatctg caccaagctc gatgaaaagt
    38221 tccaagactt catccactac ttctgctgga cgagctgatg gcttatcgat tttgttaaca
    38281 accacgattg ggacaaggtc ttgttccaag gcttttttca atacgaaacg agtttgtggc
    38341 atggttcctt cataggcatc tacgaccaag acaacaccgt caaccatttt catgatacgc
    38401 tcaacttctc caccaaagtc cgcgtgtcct ggtgtgtcca taatgttgat acgagttccg
    38461 ttgtaagcaa cggcagtatt tttagcaagg atggtgattc cacgctcttt ttcgatatcg
    38521 tttgagtcca tagcacgctc tgccaattca gtacgtgcat caagcgtttc tgattgtttc
    38581 aataattcgt caaccagggt tgttttaccg tggtcaacgt gggcgataat cgcaatgtta
    38641 cggatatctt ctcttaattt tgtcatgatt tcctctataa tattcaaaat ttattttcta
    38701 actgaacgat tataccataa tttcaaataa ataacataac tcaagcaagt gtaaatgttt
    38761 tcactccgct tttcttttca cgtcaagcct tttcaaagca agcgacttat gataagatag
    38821 gcacagtatg cgtttagata atttattagc ccaagaaaaa gttagccgaa aggccatgaa
    38881 gcaagcactc ctcagagggg aaattctagt cgatggttgc ccagcccgat ccctagctca
    38941 aaatatcgat acaggactac aagaactcct ttttcaggat cgaatcattc aaggctatga
    39001 acacacctat cttatgcttc ataaacctgc tggtgtcgtt acagccaaca aagacaagga
    39061 acttccgacc gtcatggacc tgcttccatc tgacatccag tctgacaagc tctatgccgt
    39121 tggccgactg gaccgagata caacgggact cctcctcttg accgataacg gtcccttggg
    39181 ctttcagctc ctccatcccc aatatcatgt cgataagact taccaagtcc aggttaatgg
    39241 acttctaaca cctgaccata tccaagcctt tcaaaaggga attgtctttt tagatggtac
    39301 tgtctgtaaa cccgcaaaac tagagattct atctgcaagt ccctccctca gtcaagcctc
    39361 tatcaccatt tcagaaggaa aatttcatca aatcaagaaa atgttcctct cggttggtgt
    39421 taaggtgact agcctcaaaa gaatccaatt tggggacttc acattgaacc cagatttagc
    39481 agaaggtaac taccgccctt tgaaccaaaa agagttacaa atcattaaaa actatttaga
    39541 gatgagtcga taaaacaaaa aaagctttaa aactaaagct tttttctttt atttaccgaa
    39601 aaattaaggc gattgctaca atccagttaa ctacagaaat cacaattcct aagatattaa
    39661 gaatcttttc tattttatag tctaattgtg actctttttg gtatgaaata gccaagacca
    39721 atcctatgat acccaaaatc aggcctacaa ttggaaataa caaaccaaga ataatcgaca
    39781 agatacccac aaaaagtgga tttttcttct tttctttcat gttctaagaa ctccttaaat
    39841 tttatacaaa ttaattatac tataaaacaa tatcttcatc ctatcattcg actaatttgg
    39901 aaataaggtt agctagtctt cactttccct ttccaagaat ccaagccata agaaaggata
    39961 taaatctcag aaaaaccttg ttttttcaag taaagagctg catttgtaac tcgttgcgca
    40021 cgttggtttt cgtagagaag gacaggttta tctttacgaa gggctgcaag actagttttc
    40081 aactgacttg aaggaatatt gcgtgcacca aggatatgtt ttctgtggaa ttctgctggg
    40141 tcgcgcaaat caatcaattg acccgtacga atcaaggctt caaactcctc attgtccaca
    40201 attttagccg cacggcgaat acgaagatag ttaaagccca tccacgccaa cattgctagt
    40261 ataagtgccc acaaaatcca agtaaccatt agttcttttc tccatttttc tcaatataat
    40321 ccaattctac cttgtgctct ctgcgaagaa ctgcttctgc ctctagatag tctaatttat
    40381 ccatcaaccc tgcatcgtaa atccgagata gttccaactt catcagttca atatcatata
    40441 atcgttttcc catgtaaaca ataataccaa atcgtttgag gaattgctgc acatcataga
    40501 atgttttcat aagactcatt ctagcaaaat tttgtgtttt tttcaagaag agactcacac
    40561 aatgctcctt attttcctat cttctttagc gattctaagg caagtatggt acaataaaaa
    40621 catgagaatt caacaattac attatattat caaaatcgtc gaaactggct ccatgaatga
    40681 ggcagccaag caactcttta tcactcagcc aagtctctcc aatgcagtgc gagatttgga
    40741 aaatgaaatg ggcattgaga tctttatccg caatcccaag ggaatcacct tgacccgtga
    40801 tgggatggag tttctctctt atgcccgtca ggttgtcgag cagacccagc ttctggagga
    40861 acgctataaa aatcctgtcg cccaccgcga actctttagc gtttcgtctc aacactatgc
    40921 ctttgtggtc aatgcctttg tctctttgct caagaaaagc gatatggaga aatacgaact
    40981 cttccttcgt gaaactcgga cttgggagat tatcgacgac gtcaagaact tccgcagtga
    41041 ggtcggggtc ctcttcttaa acagttacaa ccgtgatgtt ttaaccaaga tgctggatga
    41101 caatcacctg ctagcccacc atctcttcac agcgcaaccg catatctttg tcagcaagac
    41161 caaccctctg gcaaagaaag acaaggtgaa actgtctgat ttggagaatt tcccttacct
    41221 cagctatgac caagggacgc acaactcctt ctacttttca gaagagattc tttctcaaga
    41281 acaccacaag aaatccattg tggtcagtga ccgtgccacc ctctttaatc tcttgattgg
    41341 tttggatggt tataccattg cgacagggat tttgaacagc aacctaaacg gagacaatat
    41401 cgtttctatc ccactggata ttgatgaccc gattgagctg gtctatatcc agcatgagaa
    41461 aaccagccta tctaagatgg gcgaacgctt tatcgactat ctactagaag aagttcagtt
    41521 tgatagttga gaaatgataa gaaccaatat gtaggctagc aacaacctgc acattggttc
    41581 tttttactta taattaaaag tttcccctgc caacttatca gctagcttgg gaaagagagt
    41641 ataaaactta tgggctaggt tcaacaaaat cgggagattg agttctcgtt tatttttgcc
    41701 tataatcttg acaatctttt tagccactgc atctgcctct aagaggaagc ggtcaaccga
    41761 tttaagataa gttccatctg ggtcagcttg gtcaaaaaat cctgttcgga ttggacctgg
    41821 attgactgtt gtcacataga ctccataggg cataagttcg agtcgcagag catttgaaaa
    41881 accaatagcc gcaaacttgg tcgctgagta aagactagac ttgccagtag ctattagacc
    41941 tgccatgctg acgatgttga tgatatgccc tttgctgctt tccttcatac gagccgcaag
    42001 gtgacgagac agattcatca gggcaaaggt attgacctca aacatctggt gaatatcttt
    42061 atcagcaatc tggtcaaatc cctcaaaaat cccgtaacca gcgttgttaa tcaagacatc
    42121 aatcttgcca tagcggagat aaagatcagt taccagagct tctagggctg aatcgtcggt
    42181 aatatcaatt tcaatcaatt ctgcatggga ataatttccg tagagttggg ctaatttttc
    42241 cttatttcta ccaagcaaga tgagttgatc attgggcagg agtttgacca tttcttgagc
    42301 tagaccaccg ctagctccgg taatgagaat agtaggcata cttatccttt ctgtgactgc
    42361 tagatttcca cttcttccaa gtctttgacc acatggacat tttcaaaaat tgtggcagcg
    42421 tctttcttga gtttgcttat atcttttgag aggaaacggg cactgatatg gttgagtagg
    42481 aggcgtttgg cacctgcttc taccgctact tgtgcagctt gcatattagt tgagtgacca
    42541 tggttacgag caattttttc atcacccttg ccataagtgg actcatgaac taggacatct
    42601 gcattgacag ccagacgcac actggcatcc gtttttcgag tgtctcctaa aatagtgata
    42661 atcttacctg gacgtggcgc tgagatatag tctgctgcct tgatttcagt tccgtcttcc
    42721 aaaacaagat cctggccgtt tttgatttta ccaaaaagcg ggccgaacgg aacaccagca
    42781 gccttgagtt ttttagcatc cagcgtccct tctagatcct tttgcatgac acgatagcca
    42841 acacagaaaa tagtgtggtc cagctcctct gcatacacag tgaatttatc ggtttcaaga
    42901 attttaccca gagaatcttg gtcaaactca tggaaatgaa tgcggtaggg cagacgagaa
    42961 cctgacacac gaaggctggt taagacaaat gacttgattc cttgaggtcc gtagatttcc
    43021 aaatctgtct gctcttcatt ggcctgaaag gcacggctag aaaggaaacc tggcaaacca
    43081 aaaatgtggt ctccatgcag atgggtaata aagattttgc tgaccttacg tggtcgaatt
    43141 gtggtttcca gaatgcgatt ttgcgtacct tctccacagt caaagagcca aacttcgtta
    43201 atctcatcca aaagtttcag ggcgagactt gaaacgttgc gggctttaga gggctgacca
    43261 gcccccgttc ctaaaaattg aatatccatt cgatactttc taattaatca atatataaca
    43321 tggctgtgcg gttttccgat cggaaatagc gtttgccaga aaaagcagca gcttcttgca
    43381 ataaatcctc ttggctgtag cctttgagac gttttcgacc atcagccaat ctttccaaat
    43441 cagtcaaagc tgtgagactt tctaggctga caacttcctc gtcctcgaca ggcttcatgt
    43501 aaatcttacc agactcttca aagactaatt gatgggggaa aatttgcgca atttcaaaga
    43561 gcaagtcatc cgagattttc tcctcatttt cagagaaaat ccgaccaaag ccgtcactct
    43621 cataacaaaa accaaaggat ttaccagaca gattaagccg aataaaagac ttattttcta
    43681 gggtgaaact tggctcagta ttgtaaagat tcagttcctg actgagttct gcaaaataat
    43741 ccgtcgcagc ctgaggactt tttttctgat agagttctgc aaagtaggca ttaacaacac
    43801 ttggcggagg tgtaataagt gttaactgct cctgctctgt cttaccagct agaagctgat
    43861 ccagatagac cttgtccaga cttgtataac ctccatactt tagagccaaa gttttaatat
    43921 cagtcataaa attcttctaa cctccattta tttttctcgg aaatgtagcc tgtaatcact
    43981 tcgccgtctt cctgataatc acgttcttcc agaattgcaa cactctctaa atcatgaatc
    44041 ttgtaggact ttgaaaaagg cactcgcagg gtaaatgctt caaaaatttc cttaatctta
    44101 tctagcaata atgcttgcaa gttttcacga ctgtcctcag acttggcaga aatgagggta
    44161 tatggcgttt gagtaggcgt gaaatcctcc accaaatccg ctttattata aagcgtcaag
    44221 tgaggaatat cttccatgtc caggtctttc atgatggaga gaaccgtttt ttcatgctcc
    44281 tcgtggtaag gattgctagc atcgataaca tgaaccagaa ggtccacatg cttgctttct
    44341 tccaaggttg acttgaaact ggacaccaac tctgtcggca aatcttggat aaagccaacg
    44401 gtatctgtca aagttacttg gagattgcct cctagatgaa tactcttggt tgtcgcatcc
    44461 agagtcgcaa agagctcatc tgcttcatac tgggtcttac tggtcaagat gttcatgata
    44521 gttgatttcc cagcattggt ataaccaatc aaaccaatct taaaggtgct agactccaaa
    44581 cgtttttctc tgacagtcgc acgatttttc tcaaccacct taagctggcg ctcgatatcc
    44641 gtgatttgat tgcgaacgct acgacggttc agctccagtt ggctttcacc aggaccacgg
    44701 gaaccaattc cccctgcctg acggctgagc ataatcccct gaccaaccaa gcgaggcaag
    44761 aggtatttga gttgggctag gtggacttgg agcttccctt catggcttcg agcccgcatg
    44821 gcaaagatat ccaaaatcaa ctgcatacgg tcaatgacct taacaccgag aacttcctct
    44881 agattgacat tctgccttgg ggtcagacgg ttgttgacga tgacagtagt gatttcttct
    44941 gcatccacca taagcgcaat ctcttccaac ttaccagagc cgacgaaggt cttggaatca
    45001 tatttttcac gtttttgtct gtagctatct acaacgactg cccctgccgt tttcgctaaa
    45061 ctagccaatt cttccatgga gaggtcaaaa ctgtccatac cctgcaattc cacaccaatc
    45121 agcaggaccc gctcctcttt tttctccgtt tcaatcatct aaaaactcct ctatctggct
    45181 taaaatgcgg tcttgtacac cagattctcc aatctgataa aaggtgacct gcatgcgatt
    45241 acggaaccag gtcagctgac gcttggcaaa acgacgagta gcctgtttga gactctcacg
    45301 agcttcctcc aaggtctgct ctctacggaa ataaggaaag agttccttat agccaattcc
    45361 tttagcagcc tgtacattag gggaatggtc aaacagccac ttggcctcat ccaaaagccc
    45421 agcctcaaac atcaaatcca ctcgatggtt gatacgctca taaagttgac tacgttcatc
    45481 atccaagcag ataatcagcg gttcatacaa ggtctcttga ttttccaaat cctgaccaaa
    45541 atgggcaatt tctaaggcac gcatagcacg acgacgatta aactggggaa tctcaaggcc
    45601 tgcttgctcc accaaatggg ctaattcctc atctgaatat ggctccaaac tagctcgata
    45661 agctaaaatc tcctcatgag gagtctcccc acctaggtgg taaccttcta gcaagctctg
    45721 gatataaagt ccagtcccac cggcgataat ggctagcttg ccacggctgt gaatatcctc
    45781 aatagtcatc ttagcttctg aaacaaaatc aaaagccgag taagactcgg ttatctctct
    45841 aacatcgatt aaatgatgaa gaacagctgc ctgctcttct ggactagcct tggccgtccc
    45901 aatatcaagt cctcgataga cttgctggct atctccacta accacttcgc cattaaaacg
    45961 ctttgcaact tcaatagcta gagccgtctt tccaacagca gtcggtccaa caatcacaat
    46021 tatttttgtt ttcatctttt ttccttgaaa aattcccatt ttttcgttac tattattata
    46081 acacaaaaag gtcagtcaga aaaatgtgac ctcttgagga ggctggctga ttgagaatat
    46141 aaaggaggaa gactcatggc taaaggattc gctaaaggtc ttgtaacagg tgtcgcagga
    46201 actgtcgctg ccgttgcagg tgcagtatac gcatttaaaa agaaagtaat cgaaccagaa
    46261 gagcaaaaag cagctttcat cgaagaaaac cgtaaaaaag cagctcgtcg ccgcgtatca
    46321 cgttaagaaa caaaagaagt tgggattatt ctcaacttct tttttctatt cttttaaata
    46381 gctaggtcaa acttcaactg tggcttaaca gggtcataat ccaccaactc aaaatcttct
    46441 gctttgatat caaagaaatt agtcccatca ggaacattta aaaccaagcg tggttggcag
    46501 tttgacggct cccgacggag caattcctga gcttgttcaa attgattatc atagatatgg
    46561 aggttgttga tgaagtagaa gaacttccca accttccagc caaaatgttt ggcaatcatc
    46621 atctgcaaag ccacatactg catagcgttg atgtggtggg ccaccagcat atcattggag
    46681 cgctgggtca aggtcgcatc cagatagatt tccccatcaa cacgccgaac atcaaacatg
    46741 gtctgaaagg cgcacgggag cagcccatct gtttcttcga aagcttggta atcccagaga
    46801 gaaataatat tgcggcggtt ccaaggattg gttttcaact gtttgagaag cttattgata
    46861 atgtcgtgtt tcttaacaac ggcaccatag cgctcaccaa tggttcccgt gtctccaact
    46921 tcccagtcat tccagtagtg aacattgtac ttgtcattaa gcacttctaa gctatttgac
    46981 tggtcttggt aaatccagag aacttctttg atggcggatt tgattgcaat gggacgcaag
    47041 gttgtgatag gaaattctcc tttagataag tcgtactcgg aaaaggcacc agttacatac
    47101 ttggaattag caacagtccc atccttgtac ttaggacgag cctgctctga gaaaacacct
    47161 tcctttagaa tgcgttcaat attctcttta aaaatcgtat ctgcttttgt catttactct
    47221 cacctcattc attgtcctaa ctagtatagc ataaaaaata aaagaggaac attactaact
    47281 ctaggttagc attttcctct ctcattatct tattgcaata caagtgatgc tgctccgata
    47341 actccagcgt catttcctag agttgcaaga gctaatttag tagatgtgcg tacttgtggg
    47401 aaagtatttt cgtcatagac tttttgaaca ccttgtagaa ggaattctcc cgcagctgac
    47461 acaccaccac cgatgacgat tgttgatggg tttaggattg aaccaatgtt agcacaagcg
    47521 attcccaagt aacgtgagaa gttacggtaa acaatcaaag caaggtcgtc tccttctttt
    47581 gcgagatcaa agacagtctt agcagttact tcttctccgt tatcaatcaa gcgtttcaag
    47641 gctgcatcgc cttcgtattc atcggcatag cgacgagtca agttgacaat tcctgttgca
    47701 gaagcgactg tctcaagaca gcctttctta ccgcaagtac atgagattgg ctggtcaaag
    47761 tcaacagtga tgtgaccaag ctcacctgct gcaccagcaa caccgtgaag caatttgcct
    47821 tctgcgacga taccgccacc aacaccagta ccgagtgtca taaagacaac atctggttgg
    47881 ttatcaccag cacccatcca gcgctcacca agagctgcta cgttggcatc attatcgatg
    47941 aaaaatggaa tgcccaaaga tttttcaatc ttttgtttga ttggttgaag ggttttccag
    48001 ttcaagttgt aggcaccgat aacagtccct ttgtcacggt caaccacacc tggtgatccc
    48061 attccaatgc cttggaagtc cgctgctgcc aatccaagca agtccaaacg atgttgaata
    48121 gactcaatca tatcatcaac gatatgactt ccctcatcca aaatgttggt cttgattgac
    48181 cattttcctt ggatttctcc tgctgttgtt aagattgcaa atttgataga agttccacca
    48241 aggtcaatcc caataatctt ttgactcatc atattctcct ttttcattgt gtagtaattg
    48301 aaaatgctta cagtactagt atatcaaaat ccattcactt atgcaaaggt ttgcgtaaat
    48361 gaattataag gaattttttc gcctaccagt atctgttaat catctaaatg atcaatggca
    48421 tattgagctg catcatttga aaatttatca aaatcagaag ttaattgtct atacatacgt
    48481 ttttttgaca tatggaataa cgaattataa ctttttgctt ttcctaaagc agcagaatat
    48541 tcactattgc tatcattact agaagaagct gtacttgctt gaccttcctt ccaaactccg
    48601 tcagcaccaa cataataacg tccaccatca acccattcat tgacagccat tttaccatcc
    48661 gattttagat agtaatttcc ttgccatgta ctgtaagcat aagaaccatc tgatttcaag
    48721 taataccaag cttgataggt ggcatcataa acccattcac cttgtgccat tttaccgttt
    48781 gatttaaggt aataagctcc ttgccatgca ttgcgagcat aagaaccatc tgatttcaag
    48841 taataccatg cttggtaaga agaatcataa acccactcac cttgtgccat tttaccgttt
    48901 gatttaaggt aataagctcc ttgccatgca tttttcacta gatttccatt tccatcaaaa
    48961 tagtaccaag aaccgtcctg cttaacccaa ccacttgctg tggatgaagt tgtatcagtt
    49021 tgagttgtag ccgcttgttt tgctttaaaa gtacccttag gagcattttt caattcgcaa
    49081 gccacaccat catgatcgcg gtctaacttg gcagaataac caggttctcc ctcatgaaga
    49141 tccgaatatc cattagccca agcttcctta caactcgaaa aatgaatgtt ttcctctgct
    49201 aacacaggtt gtgaaaacaa ggctaaaatt ggcaacgtca ccagactcat ttttaaaaat
    49261 agacgtttat tcatttcttt ctcccataat tatgatatcg atttcaatat tattttatca
    49321 aacttatttc agcaattcaa ggaaacttct ggtcttttct acaaactcct ttggttttgc
    49381 cttattcaag acatgagggc catctgagat aatctgaaat tgggattctg atattagttt
    49441 atgaagactt ctcatagaac taagattcgg tttatctttg ataccacaaa ttagtaaggt
    49501 tggataaggg caagttcctg ctatatccgt taaatcaagt gtcttcaact cctcagaaac
    49561 tccgaccata agagccttgt ctgctccctg tttttcaaat actcttttgg gaagtagttt
    49621 aaaaatcagc aactgaagat aaaataggat attccctgct aatttaagcg ggcatcctga
    49681 cagaatcaaa gctcgaagat ttggtaaatc gtaactggaa agttctagtg ccagggcagc
    49741 acctaaggac aatccaatca aaacaaaagg ttctgtctct tgagctaggt actgataaac
    49801 tcgctcctta gcttgttgat agttactaac tccagaagga aataactcga tagcctcaga
    49861 aggataatct gtcagtagat tccgaacttc tttccaagac tctgctgact gccctaaccc
    49921 atgcaaaaat atcagtttca tctagttctc ctcaaggctt agttcataca agcctctcac
    49981 tgcattacag ccgtaaatag cttctgcttg ggctaaatct gccaaggtca agactttctc
    50041 ttctacctgt cctgtttcta gcaaatactg acggtaaatt cctggcaaga ttccaagtcg
    50101 gataggcggt gtgtagagtt ttccagcgat tttcagaacc aaatttccta tagaggtttc
    50161 aagcagttct cctgacttat tgtggtaaat cttctcttgt tctcctaggc tcaaatgcgg
    50221 tcggtgagtg gttttaaagt aggtaaagga ttgattcaaa gcagcttcct gaaggcagac
    50281 ttgggcctga caaaagcttg tactgagagg ggttaatact tgacgattga cttctatctc
    50341 tccagatttg ctaaggctga ttcgcaagcg gtaatcttga ttagcttcac aatcctgaca
    50401 ctcttcctca atcttgtgtc ccaagtcttc tgcatcaaaa ggaaaggcaa aataacgact
    50461 agcttttctc agtctttcca gatgtttatc ttcaaacagc aggttcttct ggctgatttc
    50521 ccctgtcgta atcagttgga agcgagcttg tttacgataa agaactgctg ccttttgatg
    50581 aacctctcgg tattcagatt cccatgtgct atcccaagta atccctccgc caactccata
    50641 aatagcttga cctttgtgaa gttgaatggt acgaatggcc acattaaaaa tccgtcgtcc
    50701 atttggaagc aagagaccaa tcgttccaca gtagactcca cgcggttgag gctccaagtc
    50761 cttgataatc tccattgtcg caattttcgg tgcacccgtt atggaaccac aaggaaagag
    50821 tgagcggaag attttaacaa ggtccacatc ctctcgcaac tgactcttga tggtcgaagt
    50881 catctgccaa acagttgaat actgctctac ctggcacaga cgctccacgt gctcgctccc
    50941 aacttcagaa atacggttca tatcattgcg caagagatcc acaatcatca tattttcaga
    51001 gcgatttttg ggatcctgtt ccaaccaact ggcctgttca agatcttctt ggtcagttac
    51061 cccacgctga gtcgtcccct tcattggtcg tgttgtcaac tcgcgatcat tttgctcaaa
    51121 aaagagctct gggctcatgg aaatcactgc catctcgtca tgttccacat aggcattgta
    51181 gcccgcttcc tgctctacca ccatacgatt gtagatggca aaaggattgg cacttaactt
    51241 ttgcttgagt tggacggtgt agttgacctg ataggtgtct ccctgcctca agtgatggtg
    51301 tatctgggca atggcctttt catagttctc tgcagacgtt acttcctgcc aatttgaggg
    51361 caaatcaata tcctcataag tcagaggaat aggggaggtc tctaccctat catgaacagt
    51421 aaagtaaagc aggtactctc ccagtagggg atccttgtga actgctaatt tttcctcaaa
    51481 agcaggtgca gcctcgtagc tgacataccc caccacataa taaccttgct cttggtagct
    51541 ttccacttgt gccagcaaat ctgccacttc ttctacattt ctcgttttca actctttaat
    51601 aggctggata aaggtatatc tctcccccaa agtcctaaaa tcaatcactg tttttctatg
    51661 cataccttaa gtatagcata aaataagaaa accctcatcc gcaaagcaga tgagagattt
    51721 caattattta aagattgaag ttttaaagct atttgtttgt tgaagaagtt tcttataaac
    51781 tttttctttt acttgcacaa gtttaccatt aactttatta gtaaccatat cttcttcaag
    51841 agctttggca aataatgatt ttaattcatc atagttatga atggtctcac catcaatagt
    51901 aaaaggagta aaaccagcgt tggctttttc taacaattct ttataatatt ctttcttaaa
    51961 atcagcaaac gattggaatc tgccgtcaga tattttttgt ataatatatt gatctccaaa
    52021 ttgtttccct tctttattag cttgagattt taatttatct gaagcatatc ctaagaatcc
    52081 tttatcatag ccataatatc cccagagtct aaatgcatta ttcttaaatg taacagcacc
    52141 gggtgcaccc tcacttgtgt tacctgcata gatactattc atcattttaa ttcctacata
    52201 ggcaatacct ggatcaaata cttctggctt atatatatta ttattcatca tacgattagt
    52261 catcagatta ttatcaacta agtcattaac actgtttaat gttattcctt tccaatcatc
    52321 agaactcata tttcgaacct tgtcatattg atcttttgac cctacagtgc gttcaatagg
    52381 atcaactttc ttaaaccaca gttgatttaa tttatcatca tgtttactaa tgactgcatc
    52441 accttcaaca taatcaagaa gagacaaggt atctatataa cctttcatat aattatcaat
    52501 gtcttctctt gttttaagag tcttcggatc ggtaatatac cactgatttc catcatttgg
    52561 acgatagaaa gtcatattaa ttcccaaagc accgtattca tcaaaccatg tacttggtac
    52621 tggtgtttgt aacattcctt gtgcataggc ttctgcatga gtccctgagc gacgtccata
    52681 tcctcctaaa tagattgcac gatcattaac atgagtagtt tcatgtgtat aaacagagat
    52741 tccaaactca tcaatcatac ttaatgcaat ataatttact tctgttcgcg tatccaaagg
    52801 attatcccaa ataactgcat aagcacctgt tccattgtta ccataatact taccaatagg
    52861 tccaaacaac tctctcaatg gggcatagtc ttctttatta ttcctcttac catacttatc
    52921 aggccatcca ccaggtgctt gataaccttc ccaaagagga gttactgtat ttttgactag
    52981 catattctta accttatcta aagataatct ataccaaaaa tcaaagtaat ttcgataact
    53041 gtccgctgct ttttcgatct gattttttat tccatcgaag gagctatgcc ctaacttttc
    53101 tgctgttcca acagccattg cactataatt cgagattaaa tacacatgtg atttatttag
    53161 atttaataat gggagtatca tcttccgatg agtagcagat tgtaaattat cccaaacttt
    53221 atatttcgcc gctttaattt catcgacttc cgaagcacgc tccgctatat agacattccc
    53281 ttctgtagct ttcttaaacc aatcataaat atttacatct gcagtaaata gtcgacgatt
    53341 ataatttaaa aagtttacca aattttctga taaggttgtc ttagaaataa tctcgctgaa
    53401 agcatcatat gtccgatgac cttttatatt atcttctctt gaaccgattt caattaatct
    53461 atctaataca cttacatttt taccatagaa atctggtttg aataacatta attctttaat
    53521 gttaacatca ccgaatttaa caccgtagta acggtttaag tatgttaaac ctaataatag
    53581 ggcagcttta tttttctcaa ctttatcaag aatcatttgt tgagctgcag atgaattatt
    53641 cagttgatga tcttcatttt caactaattt agttactaat atgtttaaat tagcttttac
    53701 atggtcaaaa ctttcttcta aataaaggtc tttaattgca tttactctat tagaccctgt
    53761 tctgccaagt ttttggtaaa ttggattaga ttgaagctca atagggctta agatatttac
    53821 gataccgtta attaaagcac tatgatcttt ttgaacgata ttaggtgtat atttaatacc
    53881 taagtcagtt atagtgtatt ctctaacatt acttagacct tcatcactag aagataggtt
    53941 gaagtagtct tttgttccat cagcataatg aacgataatt ttgtttgcat cacctaaatc
    54001 agttacaaag ttattattgt tcataggagt aaccgataga atctctttag tatttaactt
    54061 gttgttctta tctagtttat tagcttgatt aacgataaaa tctttgttat agaatggttg
    54121 tagtttttca atattcttat aagcaagttt atagtctgca ttataatctt gaatagactc
    54181 gtaagctgct tcacgactgc taacattatt caatttgttc aattcataag gttcacttac
    54241 gaatttagca gcagtaatat tgaaactagc aacttttgca tcagcctcag atttagataa
    54301 gtgttttaat ttatttttgt ttttattatc tttaccagtt ccatcttcag aagagctata
    54361 tccaataact ccaaataaat tagacacaaa aggatttgca tctccatcat taacatcgtt
    54421 tgaaccgaat atttcctgac ctttaataac tttcgcatat gttacattat cttgaaccgc
    54481 tccatatggc caagttttag aggttattcc accaacatat ttagtatttt taacatcaag
    54541 aatacctttt gctacagagt ttgtaagttt tccttttgtc cctgatagcc agctatgagc
    54601 tttatattga acgaaaggaa caagcatact tgcatttgcg ttatttgctg taatagtagc
    54661 atcaacgtag gctctattaa caaaccccat atagttagaa ccggcaatac ctccaactgt
    54721 tgaattatta ccagcagaat ttatactacc gataaatgcc acattttcta aagtagcgtc
    54781 ttcttttatg ttatttacga acccagcaac attatttcta gctgttatgg ttccgattgc
    54841 tttgatattt tctatacgtg tcgttccact gatttcattt gctattgcag cgatattatc
    54901 ttgattagat aaatcaatat caatattttc aaaatttata tttttaacag ttccagcctt
    54961 aatcttatcg aataaaggat gttccaaatt atggatagca tagcgtttat caccatcact
    55021 caatagttta ccagtaaact ctgaagtaat ataagatttc cctttaggaa caacgttacg
    55081 agcgctcatg ctttgaccca gtttaaattc accggcagga tttgtctgaa tggcttggat
    55141 caattcttca aagtcgtaga aaataccagc ctgactagct ttgggttttt caatatagta
    55201 ggtataagtt tcttcaaagc gattatcgac attacgttgg accaagttat ccgctactgc
    55261 tattaccttg tagacttgtt ttccatcaat tgttgactct tcaatttttt gaaccgctag
    55321 tagattggtc ttatggtcat tagaggtaac ttttaaataa taatttgaca aatcagttgg
    55381 cactgaagtc aatcgagttt catcagtttc aacttggttt tcatatttga ttaaatccgt
    55441 tcttataata tcttttattt caactttttt gagatcaatt cttagcggtt cttctttcag
    55501 aacttcttcc tcatccccct caccacggtc ataaaccatc tttgtttcaa gtttataatc
    55561 cttgtagtac tgcaaatctg tcagggtagt tgtcaagtct tctggtgaaa cattcaaggt
    55621 tttgacaatc tcatcacctt ttttcagagt tagggtaata gatttaatgg ctgccttact
    55681 tggattttct aaactatact taacatctga agtacgcttc aaatccttag attcaatttc
    55741 tgtgatggtc aacactggtt tttcaaagct cttggtacca cgtttcaaaa ttttgtcttg
    55801 aggttcctca aggatttcct ctgtaacttt tggagcgtct tctgttttaa ctcccttaat
    55861 ggtattaaag gtcttggtga tttttttctg gccttctttt ccttcttgag ctacaacttc
    55921 taagcctttt ttcagttggt catcttcttt aacaacttcc ttaaatggaa tcttttcaag
    55981 actttcttct ataagagttc cttcaattgg ttttgtacca cggattacct gttgatttac
    56041 aggctccttg gtcacttccg tactggttga aaggactttg tctgtttcca caccttccac
    56101 tgtttcgtaa gttgttttcg taacttgaac tcctttagaa ccaggatttt caactacttc
    56161 gtcacccaca tattttgtgt catccggaac tacgtctgtt gtgaaatcta attcactttc
    56221 aatagacttc tcaactgtac ctgttgttac tttatattct ggatgtactt cttgtactgg
    56281 tgcttgacct tctttgcctt tttcttgcgt acctttagct gttttagtct cctcttttgg
    56341 ttctacagtt tcttcaggaa cttctggttg cttttctggc tcaactggtg ccgttggtgc
    56401 cttttcgtct tctcttggcg cgactggttc tactgatggt tcaacttttg attcctcagc
    56461 tggtttgtct gatggttgac tttccgattt gactggtgct tgttcatctg tttttgaatc
    56521 aactgctcca cctacttctt caactggagc tgattctact gaatcttctt tcccctcttc
    56581 tactttagga agggtgtcgt cagtaggttt tacctccgat tttggttctt cctttggact
    56641 ttcttctgtt ctaggtgctt cttcttttgg agcttcctct gtctctacta cttggttttc
    56701 tgtcctagct tgctcctgat ttgttgttga ttgaggagtc tcaacttcga ctacagtcac
    56761 ctctccaggt tttgctgagg tttcttctaa aacagtgtcc aagccaagcg ttttgaggat
    56821 gtcacctgat agataaccaa catagcgata gccatccatt tcaacaacac cctctcgact
    56881 agccagcgct agggtcgcaa ctgggtctac agcccctgca ctaggaagaa ctaccaatcc
    56941 catagctcca actagaaaga cgctagcaat tttctttctc ttgtagatta aaagcaagct
    57001 cccaacagtc agcaaaccaa aagctgtcaa aacagatgct tctgtccctg tttgaggcaa
    57061 ctgatctttt tgatacacca aaccatatac aacttcattc ctgtcaggct ttcctgtctg
    57121 aattaaatct ttagcttctt gtgaaataat ctctttattt acatagtgat aggtggctgc
    57181 gtccactaca gaaggagcca tcaaaaggct tccaagaaat acagagccta caactccctt
    57241 aatcttacga attgaaaaac ggtctttttt aaacactttt atctccttta ttcattctca
    57301 agacttccta atagcatctt gcggatagtg cgcacgcgca cctccgatta attttggacg
    57361 actagccagt gccgttacat gggcatgacc aatctctctc aaaatagggc gaatcggaac
    57421 ctgaacatgc ttgacatgca tgccaattgc agtgtctccg atatccaatc cagcatgagc
    57481 cttgataaat tcaacctcaa ctggatcctg cataaactta aaggctgcca actgccccga
    57541 acctcctgca tgaagagtag gatggacact gacaatttcc agatcaaact gctctgccac
    57601 ctgacgttca acaacgagag cccgattgac atgctcacaa ccttgaactg ctaaatggat
    57661 acctctacta cctagaatac ccaagatagt ctccactatc agctcaccaa tctcttgact
    57721 ggattctttc ccaatctgac cacctagcac ctcactagaa gatagaccta aaacaaaaag
    57781 ggccccctgc ttcaaattgg tcttttctaa aacatcttcc actacctgac gtgtttctct
    57841 ttgaatctgt gtctcgttca tctctgttac ctctgttgtc actcttctat cataccgttt
    57901 tttcttgttt ttagcaagat agacaaccta gaaagtttgc ccaattacgc ataaaactcc
    57961 cagaattgac tgggagttag ctagtttcta ttctatttat atatatttca actttcgtcc
    58021 ctttttgggg tctagaatca atcttcatct ggtaattggc ttcaaaatga agtttgagcc
    58081 gttgatcgac attttgaaga ccaactcccc cacgtttgag ttgactttga ctactatcac
    58141 cagcatcttg gaagccaacg ccatcatcct caatacggat gaccaatccc gaatcctgtt
    58201 tctggacaga aagtttaata tggccctgac cttccttttc cttaatgcca tggtaaagag
    58261 cattttctac aaggggttgt aggaccagct tgggtaagac taaattatca aaggcaacat
    58321 tttcattaat ttcatattcc agcttatctc catagcgttg tttctggata aagagatact
    58381 ggcggacatg attgatttcg tcagagagac aaatcaagtc cttgccttga ttgagcgcca
    58441 agcggaaata ggttgccaag gacttggtca cctgcaccac tcgctgacta tcatgaaatt
    58501 cagccatcca gatgatggtg tccaaagtgt tatagaggaa atgtggatta atctggctcg
    58561 aaagggcttg aagttggtac tgacgggtcg tttcttcctg gctacgaata gctaccatca
    58621 actgatcaat ctgatccaac atggcattaa attggcgagt tacttctctc agttcatagg
    58681 caccaacttc cttggcacga agattttgag caccagaagc aatttccaac atggtttctc
    58741 tcaaatcctt caaaggagca atccagcgtt taagactgaa ccacactaag cagagacaaa
    58801 caagaagaga tgtgacactg gccccaagca aggtccacaa gagctgactc cgaacctggt
    58861 ctaacttttc caatgatgac acgccaagta ccgtccaatc agttcctgca atcttctctt
    58921 gactgacgta ggatttgtga ccaggagtat aaccctgacc tgtctcgatg tagggtttca
    58981 tagcctccat tttgctagac gaactataaa ctgtgtgttg aggatggtag acaaattcat
    59041 ggttttcatt gataatgaag gcaaagccct gctgccccaa ctggagttga ttgagatagg
    59101 cttccagagt ttcataagaa atatccaaac gaagcacacc aagattggct ccctttgcat
    59161 caacaagttc ttgagtgaca gaaatgaccc actgactatc tgatttacga gctggagtca
    59221 aaacaggcat agctccctga tgaatggcct tttggtacca atcctcagcc atcatatcag
    59281 aggaagtttt catctgcaca ctgtcatctg tagaaatgac ctgaccagat ttggtcacca
    59341 gcacaacagt tttcaagtcc ttatctgact tcaagatggt caaaaacaaa tctcggattc
    59401 cctcgacctt gtcttgactg ggattctcag cataggccag aacatccgtc tgctgggtca
    59461 aaccagtcga ggtggtttct agttttttga tataagactg aataaagtgg ctagtctggc
    59521 tgatggtcgt ttggctgttg ccctcaatgg tggcctcaat ggctgaagaa cttgattgat
    59581 agtagaaagt tccaaccaga gctaggagaa tgagaaagac cagaaagatg gaaataacca
    59641 ttctaactaa aagagaagaa cgcttcatcg gtcttctccc ttcttaaact gacgaggtgt
    59701 tacacctgca atctgcttaa aacgttgggt aaaatagttc atatcttcaa aaccaacctt
    59761 ctctgcgatc tcataaatct tcaaatctgt agttaaaagc aagagcttgg cttgtttaac
    59821 acgttctctc accagataat cctgaaaagg caagcccaac tctttcttaa tcaaggaact
    59881 cagataggtc ggactaaaac ctaagtcact ggctaaagac tttaaactaa attggctatc
    59941 agccagatga gactggattt tctgggccat gtttccttca aacctattag tcaataaatc
    60001 ttgtaactgc tcttctttct cttccttgtc tagtttttgt ttgattttcc ccaacatttc
    60061 ctcaatatcc tgacgagaaa agggtttgag taggtagtcg tccacaccta gtttgacagc
    60121 agacaaggca taatcaaaat catcgtaacc tgttaaaaag accaaatgaa cctgaggata
    60181 ggtttctcgt accagactgg ccaactggat gccatttaga tgaggcatgt tgatatcggt
    60241 taaaatgata tctggcacct gcttttggat caattcccaa gcctgccttc cattttcagc
    60301 ctgaccgatg atttccatat cgtaggctgc tacattgacc agtttagtca aaccttgtct
    60361 taccagatat tcatcttcta cgattaagat tgtgtaggtc atgatctgct cctttaccac
    60421 ttactagtat cagtatagca aaattctcct ctaactgctt aggaaagacc tcttatactc
    60481 aataaaaatc aaaaagtaaa ctaggaagat agccacaggt ttctcaaagt accgctttga
    60541 ggttgtagat aaaactgacg gagtcagtaa ccctacatac ggtaaggcga cgctgacgtg
    60601 gtttgaagag attttcgaag agtattaatc aacataatct agtaaataag cgtagccttt
    60661 ttcttccatt tggtctttgg gaataaagcg gatagagagg ctattgatac agtaacgtaa
    60721 gccgcccttg tcctgtggac catccgtaaa gacatgccca aggtgagaat ctcctactcg
    60781 gctccgcact tccatacgcg tcatattgta ggacttatct tccttgtagg tgacaacatc
    60841 tggactgatg ggttgggtaa aactaggcca gccacaacca gactcaaatt tgtcttttga
    60901 tgaaaagaga ggttccccag ttgctatatc cacatagata ccggattcaa atttatccca
    60961 gtaacggttt gagaaagctc gttctgtttg attttcctgg gtaactgcat actcctcagg
    61021 tgacagggtc tttttcaatt cctcatcact tggttttgga tatttgctgg catcaatgac
    61081 aggataggcc gcctgattaa cattgatatg gcagtagcca tttggatttt tcttgagata
    61141 gtcttgatgg taatcctcag ccaccacaaa attcttcaag ttttcctttt caactgctag
    61201 aggttgatcg tatttcttag ccacctcatc aaagacttgg ttaatcactt ccaaatcctt
    61261 gtcatctgtg taataaacac cagtacggta ctgggtcccc acatcatttc cttgtttatt
    61321 tttgctggtt ggattgataa tgcggaaata gtgaagcagg atttccttga gagaaatttg
    61381 cttggcatca taggtgacat ggacggtttc tgcatgacct gtttggttaa tcaattcgta
    61441 cttggttgtt tctcctctac catttgcata gcctgaaacg gcatccgtca ccccgggaac
    61501 gcgtgagaaa tattcctcca ctccccagaa acaacctcca gctagataaa tttcgtgcaa
    61561 gtctgcgtct ttactaattt ctgttttttt cactgctttt cctccttggc taactgccgc
    61621 cttttcaatt tgcgaggcat ctgtctgccc tgcatttcgt atcaatagaa catagaaacc
    61681 ggttatggct agaaaaaata ctcctagcaa caagaagatt tttaacttat cattcataag
    61741 acacctccta ggctaattcc ttcaaagttt gcaaaattgc atctttttcc atgaatcctg
    61801 gatgtgtttt gaccaacttg ccttctttgt ctataaaggc ttgggttggg taagaacgga
    61861 caccataagt ttccaaaagt ttgcctgatg ggtcaactag gactgggaga tttttataat
    61921 ccaatccctt ataccaattc ttaaagtccg cttcagattg ctctccctta tgtcctggtg
    61981 acactactgt caagaccaca tagtcatcac cagcttcttt agcaatctca tccgtatctg
    62041 gaagactagc cagacagatg gaacaccaag aagcccagaa tttgagatag actttcttgc
    62101 ccttgtaatc agataaacgg taggtcttgc catctactcc catcaattca aaatcagcca
    62161 cctctttccc tttagctgcg cttgttttac tagctgtctg ctccgtcttc atttcatctt
    62221 tcgtttggtg ttcactagtc acggacttgc ctgaacaagc cgtcaaacaa aggagcgaac
    62281 ctgctccaag aacacatgtt tgccattttt tcatattgat attcctttcc attttattca
    62341 aataattgac ttaaaattga agcatttcca aacagaacca agagtcccat cacaataatg
    62401 agaaaaccac ccactttttt gaggattccg agatagggat gaagttttcg gaaatgtttc
    62461 aaaacataac tagaggtcag agctagaagc aagaatggta gcgccaagcc cagcgtatac
    62521 accaacatga gaccagctcc ctgccaagct cctgaaccac ctgaagccgc caaggccaaa
    62581 acagacccca gaaccggccc cacgcaaggc gtccaagcaa aactaaaggt caagcccaat
    62641 aaaaatgcct gactatagcc cttaccattt tgcccctgtc cttgcagttg tagcctcttt
    62701 tccttataaa gccccttaaa gtgtagaatc tccatttggt gcaaaccaag aaggataata
    62761 attgccccag taagatattg gaaccaagaa gcataaagca aatcgcctaa aaaaccagct
    62821 ccatagccca acaaaataaa tataaaggaa attcctgcta taaaggccag agttcgtaat
    62881 aaactagtaa ctgagattga aaatttgccg ctagaagcct gagcaccatc cttatcatct
    62941 agtaacactc ctgtatagac cggtaacaaa ggtaagatac aaggagaaaa gaaggataga
    63001 atccctgcca aaaagacact tagaaaaaag aaaatatgac ccataaagtt cctcctatca
    63061 ttttattgat agatttatta tactccttcc attttcgaaa aaacagggac aatgataggg
    63121 gaaaatagga atttaatcag atgatttaga aatcttggac aaaaactata caaaaaactg
    63181 tcaaagcaca aaagcgcata gtatcaaggt tctataaagt tttgatacta tgcgcttttg
    63241 taatggataa atagttagtc ttttttaaag accggatctt tcaaactctg catactggca
    63301 ttgataaccg cgcctaggat aacaatttta gcaatcaaga taaaccaaaa catcataaca
    63361 acaagaagaa cggaacctaa aattcggaca tccaccaaat gatggacata gtaattgaga
    63421 taactagaga acagagttag taaacctaaa atcactaaga gaacaaaggc actgcctggt
    63481 agggtatagc taattttcct gttagataga ttgggaagaa aataataaag catgaccaag
    63541 atagcaaaga ggagggcgta aatcagagga cctgccaacc cttgtaaagc ctgatagata
    63601 atgccatctt ttgtccaata atgagcaagt aaagccaaaa tcatctgacc aaataagatc
    63661 aaaaacaagg caaacgcaaa gaggagctgc aagccaaaac tgactaggag acttagcatc
    63721 tgatgggaaa taagtccacg actcttttcg acgccataag ccttgttaaa agctttttgc
    63781 aagaaattca tagattttga aaaactccat aacgccgata aaacagaaaa actcaataaa
    63841 cctgttgaag gttgcgtcaa aacttctctg gctatttttt ccacaccttc atagaggctt
    63901 gggggcagaa cgtctttcat aaagcacaga aattctccca caggaatctg aaaatagggg
    63961 aggatattga ccaccaccaa aagcaggggg aaaatcgaaa tcaaccaata gtaggctact
    64021 gcgacactgg tcaactcact atctgatgct tgataataat gcaaaaaagc ttttaataaa
    64081 ggcttgtcta tcagctcttt ccaccacttt ttcatgtcat actccttcat ttataatctt
    64141 atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggttgct caaagccctg
    64201 ctttgaggtt gtagatgaaa ctgacgaagt cagtcacata tataatccaa ggcgacgctg
    64261 acgtggtttg aagagatttt cgaagagtat taactaattt cttcttacca attccaccat
    64321 atcatacggc agggtattgg cagcttcctt caaggaatag ttctctaagt tatttacatt
    64381 ttgtcgtaat ttcttggcat acttagtcgt aatcaatcgt ttttcttcgt attcgaaaat
    64441 caacttgcgc tccagataat agcctctcag catttcatcg atattgttgg gtttgacacg
    64501 attgataacc cgttcgacaa aggcaccact gctgataata gctgtttctc gaagacgaga
    64561 ctcctgcata aaactaatca aagagcgtct gtagactccc ttcaggtttt ccaaactttc
    64621 aataatcatc tctgtattgg caagatagag ctctgcaatt tggtcataat caagagcacg
    64681 gagacggctt tgctccttgt ccttccagct acggaaggtc tttccaagag taaaaacttc
    64741 atgaaggaga aaacgtaaaa tcctcaagga aacaagaaaa taataggtca gtcttgaggc
    64801 aagtttacga ttgattcctt gttctatatt tttcagataa cgttggtaaa ctcggtaagc
    64861 acgattgcta atgttcccct cttcataggc ctgttccaaa ccatcacttt caatactaag
    64921 aatcaagagt ttcaaagcag cccaatcttc ttgatcatcc tggttttctt ggcttaaaat
    64981 gagattttca atacgtccat gataattgtc aatagccgca tagaggggaa gtttatttct
    65041 ggtgtcttcc aactcttttt ccaactctag cgttacttca ttcaaaatgg cgatatgcat
    65101 gagataatcc ttgctttctt cctcttcatc agaaagatga ggcaagacca agagacctgt
    65161 taaaaagctg acaagcgtca cacctgcaac aaggaaaagc aaaagaggat actcctgttc
    65221 tagattactt ggtatcaaga gaatcgtagc aatcgacacc gttcccttaa cacctgaaaa
    65281 ggtcaagaga aacatgtcct tcatatactt atttagcttt ttcttgaggc gtcgggttct
    65341 ataggcataa tagccataga tcataataaa acgaatgaca aagaggacaa aggtaagggc
    65401 gatgagagat agcaataaaa gtagaggatt atagattgga ttggtcaaga taggttctgc
    65461 tatcatttcc aactccatcc ctaaaatcac aaagacagaa ccgttgagca taaaggtcac
    65521 tgtatgccag accgtctcgg tcaccgtatc cacttgggct tcgaggagcg tgattttctt
    65581 gaagcgactt gcctttaaaa ttccagcaac tacgacggca ataatacctg aaacatgaac
    65641 ttcttctgcc agaaagaagg tcactagagg caaactcaat tctaataaaa gttcactggc
    65701 aatatccgtt gcgcgcacac ttagcaagaa ggtatggagg aagcggttgg tcatggccgt
    65761 taaaaatcca attaaaaaac cgcctaggat tgaaaagatg agcgaactgc tagcttgccc
    65821 cagagaaaaa gctccagttg tccaagctgt caaagctacc tgaaaagcca ccaaaccaga
    65881 agcatcattc aagagtcctt cgcccttaag aatattggac acgcgcttag gaaagctaaa
    65941 acgctccgaa agagaggcaa aggccaccaa gtccgtagga ccaagggctg ccccaacagc
    66001 caagcaagct gccaagggaa ggctgaacca aagaagatgg gccaagccac ccaaactcaa
    66061 ggtcgagata aaaatcactg gaaatatgag ataaacaatg attcgccagt gttttaaaat
    66121 agccgtaaca tctgcttctt cagcctctcg gaaaagcaag ggtccgataa ccagtgccaa
    66181 aaacaactcc gtattaaggt gaaagtcagt attgggtaaa aagagaccaa tcacaattcc
    66241 caaaagaatc tgcaccaaag ggagaggcaa aaagggcagg agcttattgg ttgtacttga
    66301 gacaatcaaa accagtaaaa ataggatgag gtaaatcagt aattccacgc acgtcctcct
    66361 taatcttttt tacaacagga ttcaaatatc tccttctgct ctttgatttt ttggtcaatc
    66421 ttggaacagt ctttgtgctc aatttttctc tggcaccgtt ccatttcaag agcaactaat
    66481 tttttcttga ttttaagcat ttttttgctc atatgcgctt ggtctagcac gcccaccgat
    66541 cgttcgtggt gggttgattc aacaaaattc tggcgcatgg catccagctt ttcgtgtagg
    66601 tattgtttat ccatgtctgt atctctctaa tttttcaatc atcactaaaa acggcgggtt
    66661 gttgacttgg tttaaagttc ggtaaatggc agctgtgtac tcttgttggt tcaactggat
    66721 cacaaaatcc aagacagcat ctctctcgag atcgcctcct tcatgaccat agtaaatcat
    66781 aatagcaatt cgtccacctt tgacaagtaa gccacatagc ttttctaatg cctcaatcgt
    66841 tgtctgcggt cgggtgatga cagacttatc agctgacggc aaatagccca gattaaaaat
    66901 ccctgcctta gcttttatca caaactggtc cagtgtctca tggccttgca agattaactg
    66961 ggcatttgtc aagtcagcct gatgcaaacg ctcttgggtc ttttccaagg cttgcttctg
    67021 aatatcaaag gcatagactt gcttggctag cttggctaaa aaaagcgtgt catgaccatt
    67081 tcccatagtc gcatccacta cgacatcctc ttttgtcacg acctcagcca aaaaatcatg
    67141 tgccatctca agtggtcttt tcattttcaa actcctgttt tacagccttg catccttgaa
    67201 cacttccacg acgtcgcatc tccatctcaa tgctgttgag agcttcccat ttattgaggc
    67261 tccacatagg cccaagcagc atatccctag gcgcatctcc tgtaattcga tggatgacga
    67321 tatgtttggg aataatttcc agttggtcac agatgaccct gacatattcg tcctgactca
    67381 tcaattgtaa acgcccctca tggtaatctc gttgcatacg agtatttgtc ataagatgga
    67441 gcaaatgcag tttaatccct tgaatatcgt tatccgtgac acaacggcgg acattttcaa
    67501 ccatcatctc atgggtttca ccaggcaaac cattgatcaa atgggaaaca atctcaattt
    67561 ttggatactt tctcaaacgc ttgaccgttt ccacctacaa ttcataagaa tgcgcacggt
    67621 taatcaggtc agaggttgct tcataagtag tttgcaagcc caattcaacc gtcacatgca
    67681 tgcactccga taactcagcc aaatattcga tggtttcgtc tggtaaacag tctgggcgcg
    67741 ttccaatatt gattcctacc acacctggct cattgatagc ctgttcataa cgctctcgaa
    67801 taacttccac cttttcatgg gtgttggtaa aattttgaaa ataaaccaga tacttctgaa
    67861 catctggcca cttacgatga ataaagtcaa tttccttata aaattgctca cggataggcg
    67921 catctggtgc cacaatggca tctccagaac cggaaaccgt acaaaaagta cagcctccat
    67981 gagccacagt tccatcacga ttgggacagt caaatcccgc atcaataggg actttaaaag
    68041 tcttttctcc aaagagtttt cgataataat cattcaaggt attataagat tttataactt
    68101 tcattataac aaaaatcacc cacaatctca aaagcctgac tttcctataa attcctctgt
    68161 ttctcgtttc cattagcctt tttttatgat acaatatggg tatgatttta atgaaattag
    68221 catctatttt attattgata ctgaccttag tcgtctgcat tatcctaacc aaacttttta
    68281 gattaaaaaa actaggacga aactttgcgg atttggcttt tccagtcttg gtatttgagt
    68341 attacttgat tacagctaaa acctttaccc ataatttcct ccctagactg gggctagccc
    68401 tctcgttcct agccattatt ctcgtctttt tcttcctttt gaaaaaacgc agcttttact
    68461 atcctaaatt catcaaattc ttctggcgtg caggattctt attaaccctt atcatgtata
    68521 tagaaatgat tgttgaattg ttcttaatga aatagtcgaa tccctaagca ttttctaggg
    68581 atttttgctt tctctacaaa atagtataga caataacact atacaatttt atacaaagaa
    68641 aagagtctgg gacaatagtc tcttatatcc aaaaaggcaa cggatttgcc gttgcttttt
    68701 tgcatggtta cgatagtctt ggtaaaatag aattgcttaa taaaccattt agaaaggcta
    68761 tcccatgcat attcactata acacaaatca aacaacttta ccactagaaa tcagttcctt
    68821 cttaccacaa gaccatctcg tctttactat tgaaaaagtg gtgaatacct tggaggaacg
    68881 tcacttctac gccttctatc atgcctttgg tcgcccgtct tatcacccta aaatgcttgt
    68941 atctactctt ctatttgcct attcacaagg gattttctct ggtcgaaaaa ttgaaaaatg
    69001 gaagagttag tgaccttaga ttgtttgttt attgacggaa ctaagattga agccaatgcc
    69061 aacaagtata gttttgtgtg gaagaaaacg acagagaaat tctccgccaa gcttcaagaa
    69121 cagatacaag tctattttca agaagaaatc actccccttc tgattaaata tgccatgttt
    69181 gataagaaac aaaagagagg gtataaacag tcagctaaaa acttagcgaa ttggcactat
    69241 aatgacaagg aggatagcta catacatcct gatggctggt gctatcgttt tcaccatatc
    69301 aagtaccaga aaacacagac agactttcaa caggaaatca aggtttacta cgctgatgaa
    69361 cctgaatcag cccctcaaaa gggactgtat atgaacgaac gctatcaaaa cttgaaagct
    69421 aaagaatgtc aggcgctttt atctccccaa gatagacaga ttttcgctca acgcaagatt
    69481 gatgtggaac ctgtctttgg gcagataaag gcttgtttgg gttacaagag atgtaatctg
    69541 agagggaagc gtcaagtgag aattgacatg ggattggtac ttatggccaa taacctccta
    69601 aaacatagtg aaatgaaata agaacaggac aaatcgataa ggacagtcaa atcgatttct
    69661 aacaatgttt tagaagtaga agtgtactac tctagtttca atctactata caataagaga
    69721 acgactcaaa attaaaaagc tagagttccg caattggaaa tatctagctt ttttgtggtt
    69781 gagaactatt ttgttccaga ctctttatct tctatttagg acaagagttt ttctttggtc
    69841 tttaatgata aagaaggtat caaaatttct agtcttcttt tttaccttta gtaactacta
    69901 atcctgcact caaacctaga agagctaaac ctgctgctac tgctgcttgg cttgccgcac
    69961 tacctgtatt tggtaactgg gctttattag tttgactagc ttcacttgaa tcaattggtt
    70021 ttgtatctgc tttttctgac acttgtggtt ttttagcttc ttgagctact ggtttggttc
    70081 caaccaagac gatgcggtct gtcggagctt ctaccacttc acggagtttt tcttccttac
    70141 ttccatcagg attaatcgct gtaaagatac gttctttacc agtttttcct tcttgttcta
    70201 cacgagtttc acctagatac agtgttgaat cttttttctc aactgtcttg taggccaaat
    70261 ctttttcaac aaattcgagt tttggaagat cttcttgtac agcagcaact gtcttctcag
    70321 aaactggttt ttccttagtc aagtggatac ggtattcctt gacttgtttt ccactttctg
    70381 aaacgaggcg aacaagtact ggaaggctat cttctccact atctaccaca gttgaagcta
    70441 cttgattgtt ttcttcaact gagacttttg gccgttgacc tttataggta atttgatagt
    70501 cttgacgatt ttcagcgaaa tcagcaagtt cttttccatc tacaagaatc ttcgattgcg
    70561 tgctttcttg aggcaattca cttggtgcaa ggaaggtcat ctcaatcatc gcaacaccgc
    70621 tcttatctgc tttacgctcc atacgccatc tcatagcttt ggctttgaca gctttaaatg
    70681 ttacgttgat ttcatcacca gctgcgatgt ctttatccgc acgataaggc acagcttccc
    70741 aattttctgg attgttgaat ggatggtctg cgtcgtaggc ttggtagttt gaatagtagg
    70801 ttggcacttc aaactctgga ccgacatagc gttctaaaac gagtttagtt ggtgcatccg
    70861 taccactatc tgcaaagaag tgaagtttgg cttgagcaac agtccgttct acaatcttac
    70921 cattttcacg gaagatcaca cccgctgata cttctggatt agaagatggt gttggtgacc
    70981 agtttgtcca acgacgattt tctgaatgat ctccgtcatt gagatagtca acgcggtcat
    71041 gagagttttt gtcaatatca tttgttgctg aagcaaaggc ctggttactg ttttcatcat
    71101 agttagggtt atctgaaaga gcctcaccaa gtttgtctgt cactcgtaca gtgacctcag
    71161 caacaaggtc actaccaagg acacggcctc gaatagtaaa ttgacctgct tttgtcagat
    71221 tttccgctgg aacttcttcc cattcaactg tcaggtcttt tgtttcgtag ctgtctttac
    71281 ctgtgaagta aactggaacc ttagtcggca attcaagtgc ttgacctact tgtagcaagc
    71341 gagcttgttt aaccgcagca actggtttat gagaaagtaa gttcttatcc ttagtgaagt
    71401 gcagacggta ttctcctaag atatcgccat tttcagcttt cgcgatgaca cgaactggct
    71461 caccttcacg aacgcttgga acgacggtag cgagaccatt gttgctaaca cttgctgtga
    71521 ctgccggaac ttttccatct acagactcaa ggtagtagtc tgtcaaatca gggttgaagt
    71581 ttgctaagtc tttgccgtca acttggattc ttgtttgtcc ttgcttggct gccgcaactt
    71641 gtttcgcaaa gatttgtacc tctgtgatag acgttccacg cttgttatct gctttaacca
    71701 tgcgaatacg aacagcatag gtttcaactt tatcaaagct aaagtggttc atttctccag
    71761 ccttgagttt agcaggggct tttagattag taactggttt ccagttggca gaatcattaa
    71821 agacatggtc ctcattacca acaaaactag ggtttttagg agctgttggg acagtcttgc
    71881 caacataata ctcaatcaca taagacttcg gtgcaccaac tccatggtct tcgtggaatc
    71941 cgacacttag attatcaacg gagcgtttgc tcaagatacc tgaatctcca aacagaacac
    72001 cgactgaagc ttctggatta ctacgattcc agtttgtcca acgattggct ggttggttat
    72061 tgtaggaaat gagcttgtca ttaacatttg aaactgggtc gcttggattt gaatctgaag
    72121 caaaggcaag tggcaattct gaaccggtcc attggtcaga aatgtttgca ccttgctcag
    72181 tttgagcaga tacgcgaaca tgaagtttag ttgttaattg agtaccttct aagcgaccat
    72241 taactgtaaa gacaccttcc ttagcgtatt gctctggacg aatcgcatcc catgcaacct
    72301 tagctgatga aacgtgacca tttgaatcat atgtccgaac gctttctggt aattgtggtg
    72361 cttctgcgat tggagttgtc acactgactt cttcaactga aacgatacct tctacagaga
    72421 cttttgcacg cgcttcaagg tcaattcctt caactttacc tagtacttca aatatttgat
    72481 aggagtctag tttttctttc ggaatagctt gccaagtgac tttatgagtt ttagggaaac
    72541 ctttgtcata ctcaactgtt actgttgctg gaagacttgg ttcctgatgc aaatctgtga
    72601 ctacatttac aggacggata gattgcgcaa tcttcttctc agtattggct tggatagtga
    72661 gttcaacttg gcctttagct ccctcatatt cagcgttcag agtgactgct cctggcttat
    72721 gcaactcaag cattccttta cgaattgcga cttccccttc accacttgta gagaaggtta
    72781 ctttatcagc tggtaataca gcttgcgttc catcttgata gtgagctcga accgacaatt
    72841 tgacagtttg gtcttctttg agactgtcag ctttttccac ttgcaagctc aagtgagcaa
    72901 tttttggcgc ttcttcaagg aattgaattg cataggtttg aagagggcca ccatctttag
    72961 gctgaataaa gatgctcgca cgcatgccgt ttgctgcgct tgcttgaaga actgtcacat
    73021 cagcattttc agcacttgct gtgacttctg gcaattgggc accataagat agagtacgat
    73081 attgcattgg ttgtcgacta gtaagacctg ttactgcttc acctccaaca gtaacagttg
    73141 gcactacagg tgctgcagga ttgccttctt ctaccacaag ggttgcatga attggttgac
    73201 cttctaaata accggtcgct tgaatacgag aacctggaat tgctaactta gctttatctt
    73261 cttcggcaat ctcccacttg tccacttcat actcttgtac acttccatca gtcaaaacat
    73321 aggaaacaga tttgtctaca gaattcaagt tagtatttgg agcaatacgt ttcacaactg
    73381 gtagctctga tttaagagca atcacttcta cacgagcttc tacttctcgt ccgtcagcca
    73441 tacctttcac cgttacaata ccaggcttgc tcacatctac taaagaccag gttacaggac
    73501 gttctgcacg actaccatca ctgtatacaa acggaacagt ggtaggcatt tcaggtgcct
    73561 ctccaataat ggtctgtact tttggcacct ctgtccccaa aacagtcttt tcttgtcctt
    73621 ctttcttacc agtaaagaca gtgacttggt tcgatttcaa gagatcagag tgggcagtaa
    73681 gggtgaattt ccctgcttgt tcagttgatt tgacaatggc aacaccttta ccattaaatg
    73741 ctttacgaat ccaagaacca tctgcttgcg ccttatagcg ttcacggctg gcttgttctc
    73801 cgttatctac accgaccagt tgaccttggc catgcaattg gaagcgaacc agattattag
    73861 cagttggaac cacattcccc tggctgtcaa caatttcata gtagatgtaa gtcaagtctt
    73921 ttccatctgc tgcaatcgca tggtcttcct taataagacg aactgccgct ggcttaccag
    73981 cagtcgtaat cttatctcga gcaatttcct tgccagattc atcacgagca attgcttcca
    74041 aggtacctgg ttgataggca actttccatt caagataaag ttcattagca tttgcacctt
    74101 cttggtaagt ccgcccatcg ctggtttgtt ttttattgaa agtcttaaga ccaagagatt
    74161 ttccattcaa gaacaattct acactagaag cattcgaata agcacgaact ggaatcttac
    74221 cttctgagtc agctactttg gatgctaatt ctttgttttc ccagttccag tgaggaagaa
    74281 ggtgtaccat cggtttcttc ttaacagaaa cccattggct ttggtagaga tagaagtcat
    74341 gttttggaat gccggctgta tctacgatac caaagtaaga gctcttaaca ggagtttgat
    74401 tttggttgtg ccatggtgta ggttcaccaa tatagtccgt acctgtccag ataaactgtc
    74461 cagcatagcc agcgttgtca cggtcaaaag tccatgaagc ggttgctgtt ttcccccaac
    74521 ccacacgatc atttccataa tctgactgtt cataattacg ctcaggtcca ttgctatgtt
    74581 tcaattcacg ttcagggcga tagtaacttc cacgtgtacg ggttgctgat gatgtttctg
    74641 aaccgtaaat caaccatttt ggatgcttag ctctaagggc tttgtaatta tcttcagaat
    74701 agttaaatcc aacagcatcg agttcatcag caattttctc atgccctccg ctaccattac
    74761 cgaaacggaa tttatctgct cccatggtaa catagcgagt cttatcaaca tccttgataa
    74821 ccttgaccaa acgtttaaca gttgctaaag agtgggcatc accattagct tcacctattt
    74881 cattaccaat tgaccacatg aagatagcag ggttgttttt gcctctttcg accatggtac
    74941 gtaggtcaaa atcagaccat ttttcacctt ttcgagcttc tgggtgagtg gcatcttttt
    75001 caaagaaacg tccatagtca taaggtttct tgccaccata ccaagtatca aaagcctctt
    75061 cctgaacgag taaacctagt tctgctgcga tttgcaaggt ttgctcacta gcagggttgt
    75121 gggttgtacg gatggagtta actcccatct ccttcatttg tttgagacgg cgatattctg
    75181 ctttatagtt ttcttctgct ccaagcgccc catggtcgtg gtgcaaggat actccatgga
    75241 atttaatacg ttcaccattc aaagagaaac cttcatttgg agtccagtga tagtaacggt
    75301 aaccaaacaa atccttctta gcatcaacca attgaccgtc acggtaaaca cgcgtaatca
    75361 attcgtacaa ggcaggtttg tcatttaaaa cggtccagag ttttggtctt tcaacttcta
    75421 aaatcgcatc taggcttgtt gattcatgtg cttttaaggt acgactcgct gtacgaacta
    75481 agcctgttac agcatgacca cctcgttcaa cgatttgata ttcggctaca agttcatggt
    75541 ctttgtcgtc cgtattgacg attttgctgg tcacatgagt ttcaaccttg ccatgttgtt
    75601 gttcttcaag ttttggtgtt aaaatagttg tcccattttt ctcaacatgc accttatctg
    75661 tcacttgtaa agtcacatca cgatagatac cacttcctga ataccaacgg ctacttggct
    75721 gtttgttgac tgcatggaca gcaatcacat tctcacgacc atctttgtga aggtatttgg
    75781 tgatatcgta tgagaactgg ttataaccat ttggataatg ccccactaac tgaccattga
    75841 cataaacttg agaatccatg tagacgccat caaaagtaag gcgaacattt ttcttgaggt
    75901 ctttttcatc tagtttgaaa gtcttgcgat accaagcttc cccaccgttg agctgtccac
    75961 cttcattttg tgcaggagat tcatgatcga aatcgttaaa gatactccag tcatacggta
    76021 aatctaattt tttccacgta gatacgtctg catcaggttt aatggcttcc ttagaatttg
    76081 cattgagttt aaagtaccaa ttttgattaa aatccacttt cctgtcttca atcatttgat
    76141 tcacttcttc atttgttaca gctttagcat cttccttgag cggtttttct tgacttgaag
    76201 cttgtgattc tatccttgga gctttttctt ccggtttagc agacactttt tcctcttttg
    76261 gagttacggc ttcatcttct ttcttctcag atgcaatagc ctcagttgaa ctaggttcac
    76321 tttgttctgt cctttcaact acatttttag tttccaaagc tttatcagcc ttttcttcta
    76381 ctatcatttt ttcctcttta ggtttctcag cagtatgagt aataagtgtt tcatccgcat
    76441 aaactacaga ttctccagct atatttcctc ctaatagaac tgcacaagtc ccaatcatta
    76501 ctgagcaagc tcccacagca aacttacgaa tgctataaac tcttttccga ttccaatggc
    76561 ctttccccat aaaaccctcc ttatattata tttagtgcag ttagctacta ccaaagccca
    76621 agtggtatac atggtatgac aacctagttt caacaattta cactctgcga aaatccaatt
    76681 caaacttcgt cagtgtcgcc ttgccgtaga tatgattact gacttcgtca gtttcatcta
    76741 caacctcaaa accatgtttt gagctgactt cgtcagtttc atctacaacc tcaaaaccat
    76801 gttttgagct gacttcgtca gtcttatcta caacctcaaa actgtgtttt gagcaacctg
    76861 cggctagctt cctagtttgc tctttgattt tcattgagtt tatattttat aggagcgcat
    76921 tattttgctt ttgctgcgta ctcttcgtta cgtttgatca tttgttttct gtaccaagca
    76981 aagataccga tatagaatac aaggaagact actgcaccaa ggattgcttt gatatcacca
    77041 gttgtagtgt taccaattgt ccaaccaaga agtttttcga ttggtccttc aagagtagag
    77101 tgagtaatca attgagtttg gctcacacct tctgggaagg cacctacacc tttagcaagt
    77161 tctgttgcaa atggtgcaat aagtgtacct gaaagaagga agagtggcaa caagagtgtt
    77221 ccgaagataa tcatacggag caatttacca cgagttacaa ccaagagagc tggagtaaca
    77281 cccatagcga tgatacctgc aagtggcaag ataccatttc caacttttga aagaagcact
    77341 gcttcaatca acatgattgg tgcaagtacg ttggcacaag cccagatttc agcacgacca
    77401 gcgatgaatg gccagtcaag accgatattg aatttacgtc cttgaagacg tttagtagca
    77461 acgtttgtaa taccttgtga tagtggttct acggctgcga tgaaccatga accgataagt
    77521 gagaagagtt ccaaagatac accggcagtc aaaccaagag acaaccatcc tttgataaca
    77581 agacgccatt tatctgcatc tgcaacacct gcaattggat gtggagttcc cataataccg
    77641 ataacgatac caaggatgaa accgatgaaa aatttagatc cccagaaacc gattttcttg
    77701 ttcaatttag cagcatcaaa gtcatattta tcaaggcctg ggaagaattt ttcaaaaatc
    77761 ttatccaaaa ccatgataac tgggttcatc atgtagttca tgtgagttga tgtcattggt
    77821 gatgaacttg gggcgttaag aaggtcatca aatgtaggtt tcatcaagtc agagttgata
    77881 attttcaaca caccgacaag gacgatagct gctgtagcaa taaagagtga aaccccttga
    77941 ctcacaccat tgttatcagc ataccattta atcaagagac ctgtgataga caagtgccag
    78001 atatcaaaga tatcgacatc aagtgtatcg gttttcttca tagcaagcat cactacgttg
    78061 acaatcaaca tgatgagcaa gaagtatagt gtccaagcag aaccccaagt gattgttgcg
    78121 agtggtgccc aaccaacgtc ggtaatactc aattggatac cagtgttttc aacgaatttt
    78181 gcaagtgatg ctgagaaagc accatttagc ataccgatga tagcaccgat acctgtaaga
    78241 gcgatggcaa gtttgatacc accttcaagc gctttggaga atttcactcc aaaaagtaaa
    78301 gccaatactg tcaaaatgat taacatgatg acaggtccac ccatttctaa aatgggattg
    78361 aaaacctttc cgattaggtc aaagattgca tccataacag ttcctccctt ttttatgtta
    78421 tatgaatgtt aacaaattag aattagctta atccgtgttc tttaatagct gcttcaatat
    78481 tgtcaaatac tggagcgctc attgctggga tacggaataa gattggccca gcttcgataa
    78541 ctgggatacc tggttcaaaa ccaaggtctg ttgcagcgat tggtgtaaag atatcgtaac
    78601 ctttcataag gtcttcgttt acatctttca ccatgactgc atcacagtga acatcataac
    78661 cacggtttga aagttcttct tctagagcac ttttaatttg gtgacttgag ttaacacctg
    78721 caccgcaggc agcaagaatt ttaatcattt ggatttcctc cgattttatt ttttaataga
    78781 caagattaag cggttgcttc agcaatgtaa gcataaaggg cttctggttc agaaattttt
    78841 gataggtctt caagatgacc atttcctgtg aagaagtcca ttaactgagc aagaatgttc
    78901 gtttgacttg aacttgagtt attgatgata aagaagagca aggatacttc tacttcctta
    78961 tctggcgcaa tcatattatg gaaagtcacc ggtttctcta atcgaacgac cacaactttt
    79021 tcagccaaat tatgaacaat atctgtgtga ggaatcgcta catttggcaa gtcctttcct
    79081 agaaattcca tatctaaacc agttggaaat gacttttcac gcgtgatcaa ggcttcacga
    79141 taagttggag tgacaatttc tcgttcttcc aacaagcttg ctacctgatc aaagagttgt
    79201 tcttgattat ccgcttctaa gcaaaacaca aggtttttgt caaagaaata atctaatacc
    79261 ataaggtttt cccttctttc cattaacttt atgccataag tataacacta tatgaaaccg
    79321 ttgtcaatta ctttctgttc ttttttgtct ctttttttat atttttgttt tgtttatagt
    79381 ttgttatata aaaacaaaca cacaaacaaa tactccaagc atttttgctg ttctaatatt
    79441 caatgaaaat caaagagcaa actagaaagc tagccgcagg ttgttcaaaa cactgttttt
    79501 aggttgtaga tagaactgac gaagtcagtg atcatatcgt aaccgtccaa tagacttcgt
    79561 tattgggcgg ttacgaaaaa cttccttcca aataaaaagg aattaagcaa ccaaacgcta
    79621 cttaattcct tgaaattgtc tcatcacctc tattagcctt taaattttga tagactctat
    79681 tttctaatca atatgaccaa tattgtcaat actaaccctt tcaatcaatt cattagttat
    79741 ctaaactaag tccattaata actccaaata gtatcaatag tgaagtaaac taaataattg
    79801 agttataaat ggctgttttc acaaaactat gtcatcacct cactatctaa tctcagtaca
    79861 taaaccattt ttttctatag cttttatctt aatcttgatt ttttttcttc aatccaagaa
    79921 aaattcctac tataaacatt attccggcag ctaatagtgt ctgggctccg cttgctgttc
    79981 cagtttttgg caacttatta ggattgttag tagttgattt actactgata ttgtttttat
    80041 caagaactgt agctgtaaca tccttagttg aatcagattt ttgtgaatga tcttctcttt
    80101 gtaaatcctc ttttctgtga ctttcattta attgactatg gtttacttgt gggttatctt
    80161 tctttttcga tacatcaaaa gtaggtttat tttcttcctc ctttttctcc tctattttct
    80221 tgaatacagg ttttataaag gtatcttttg atagattaat aacatagcca gcgtcttttt
    80281 taccttcgaa accagaaatt tcccaaccat caaattggta tcctttttct aattcaccct
    80341 tataaacagg taaaataaaa tcttcttccg acactatcgt tgaactcatt tcttttccat
    80401 tttgaatggt cacagttacc ctatgaggtt ttaattcact tacctctccc gtatctttat
    80461 ttaaaatgaa ttcttttacg gttgtatttc ttgcaaaatc ttttacaaca atcttaatgt
    80521 ttagtgtctt gtctgttatt tgtttaatat catcaaatga tgtatattct tttccattta
    80581 tataaatatg ttgttcttga atctcaccat cgaatccatt atttctttta tcattgatgt
    80641 taaagactac agattttcca tcagcatatt cgatactagt atttccctta ggatcaatgt
    80701 ttacttcggg tttaacatta tcatataaaa actgatagtg gactccaact gctttagcat
    80761 tcaaattgct atagccagtt tgaagataaa catttccatc catatctgtt accttatctg
    80821 gaaatccgtt tgctttatag tctttcattc cccagtccat gatgtcaccg tctttaacat
    80881 tcagcttaat attaaaatct ctagtgttat caatgtgtaa atctccgtag attaaataat
    80941 tatctacaac cgattcatta actctcaatt cccagttaaa accaccctta tcagaaatct
    81001 tacctcttaa ataaaattct ggatttcgta cataaatttt attagattta gatggattaa
    81061 agtagttctt atccattgaa aggtttactg gtttggtatc aataaataac atggagccat
    81121 cttcttttat agcttctaca ttggacttat cctctccagt gtattcttta tcatccttac
    81181 caaataatac aagtttagaa gaatctgtca caagatttcc gtctttatcg atagcttccc
    81241 ctttatcgtt cattttaaat gtaaacactt gataccttat aatgttaaag ccgtccaaag
    81301 ccgacattaa tacagcttgg gtacttcttc catcttcaac atttctacta tcagcataaa
    81361 ttgttgtttc tgaaaggact cttagattag gattggcctt ttgtattttt gctatatctt
    81421 ccttgctata gactccattt ccttctaaca tatccgtttt tccaggatta taggtagtca
    81481 cttttagtgc atagcctttt cttagaatga tgttatcctt taacagatat tgttgttttt
    81541 ctgaatcaga atagatttta ccagattcca ttttagttaa attgtctggt ttgttttttg
    81601 aaagatctcc ttcccctaat tctatgacat tcccataact tgatacatag ggatattctg
    81661 atttagtttc cttaattttt tcaggcattc taattttaat ttcagctttt ttctgatcat
    81721 tatctttaac aaataatctc atatctcctg caaaagctaa tccatccaca atatcattaa
    81781 tattagcgta tagatcaaat gtcatcgttt ttgagtggaa atcatacttg gtcgctttga
    81841 tttctataga tttatagtta ttcccataat ataccttggc atttttagaa acattactta
    81901 tctttccaag aatttcaaag tgtccatctt tagacggact tagaacacca taaatttttg
    81961 atttgatttc gtcaagtttc tcagtttcat attctagatc agtcccatca tcgtaggcta
    82021 ttatatttcc tttatcatcg tatttataat cacgctctac tgttcttgtt ccaccattta
    82081 caaagtcgtc ttcttgctta tatttctttt taatgagttt ctttaggtct ttattttcaa
    82141 agtctctaat tgttgaaata gttttatgaa tattaaagag agattttcct tcgatagact
    82201 gattatctac taaaaggtta ttttccacct cagctgtctc tttgtttagg ctatcatctt
    82261 taacattttt taaattctta aatttagatt caggaatttc gccaagcttt tggtatttag
    82321 atgaatcctg atcaggatct actagataat aggaaatcat ccctttttca tcagcttgat
    82381 tagcaaattt aatcctatgg attttggtaa agttgcttgc tccatctagg gcgattactt
    82441 cgatgatttt tcctcttaga tctcctgctc cgctaatttc atagatagta ttcccatctt
    82501 tatcaagctt cctatttctt ccttgaccct cacctgcata agttacttca agatttttct
    82561 caacctctcc atcttcatta acaagagcag ctcctgcata ccacacctca tttgcaatct
    82621 catcaaattt ttcaggatgt tctttttgat ctctagcaaa taaagtttca ttcttgtatt
    82681 gatcttttac cttgtgataa gtatcctttg tgattagctt aattttttca ggatttgaaa
    82741 aatcaaccga aacaatctta ggggctgtgt tatcaatttt tacaggaata taggaaacct
    82801 gccatgggta atctttagtt aatctatatt taaatttata gaaatattga ccttccgcaa
    82861 tcggttcaaa ttgtcctcgt atcttagtag ctggatcttg gttatcctta ctttctgggg
    82921 cattttcttc tctacctctt gggttataga tgagtccatc ccatttcaag tcaccccaaa
    82981 cttttagttt ggatgatttg atacccttag cgtcatttcc cttagaattt agaattcctc
    83041 ttatgaagtg ctctcttgat acaactttaa ggtctttttg gttttctcct tctttgttgg
    83101 tgtttacaat tgaaattagt ccttcttctg cacttcttag aacaagtgga gatggagtta
    83161 gtcctctttc tagttgagca tcttgaggat ttccatttga atctaaagga taaatgttag
    83221 caatcttaga accactagtg gattctgcat gaacaccttg gttgttaaag gcaaataagt
    83281 ctggatcttg atctagagat gttctatttt tatcttttct attttggata actcctgctg
    83341 gattaaattt atctatacca tgttctccac caatcccctt atttaaggtt cctggaattt
    83401 ttggtttacc gtcatcatca taaccttcca ttgtttttga ttttgatcct tcttcccaag
    83461 cccatttatc aaggattggt tcgtggttcc aattcccagc aaatcccatt aggggcattg
    83521 ataaagatgg ttggaagtcg gttttcttgc cattggagct taatgcttcc atttcttcta
    83581 ctgactcaaa atgaataaat gattctacaa atttattttt atttttagcc tccccaacat
    83641 ttataaccgc atttaaatca aagctagaat ttgcccctat agtgaaagtg tcatgctcaa
    83701 atgtaatatt tgcccctttg actttttctg gatgaatttc tggaacaatt tgcttaccat
    83761 ctggagattt ttcatcttta tatgtttcat ccagttttag tctgtcagtt agagaatctg
    83821 tagtcaccgc tgatgctgaa actttaaagg ttaaaggcct gttggatgta ttgtgaagct
    83881 taattgtaaa gtatttttta tcacctttta tttctttaag agaaatggaa ccatatgaat
    83941 ttaccaaacc tttagaatcc gtgtttttga aagtcgctac aacttcattt ctcaaagcgt
    84001 tggccacatt aattagcccc gccccctgct gtctaggtga tgcaaagtat tgactttttt
    84061 ctttccaaga agttgcatcc atcataggtc ttgcagtatt ttgtagggct atttttgtaa
    84121 gacttgtaag gtctatttta tcatcacctt taagattttt taatacgggt ttttcaagca
    84181 tttccttcaa ttttggtcga atcaaaacag tagaagctgc tacgattgga gtcgccatac
    84241 tagttccgga catatagcca taagttgatt tgccattaat aacattaagc gtggatttaa
    84301 tatttttacc aggtgctgaa acatcgggtt ttaaaagtaa atctattctt ggcccccaag
    84361 atgtggatcc tgctggaacg atgatatctt ctttatagag ttctttgtct gtgtcaggtg
    84421 caaacttaaa gtcaatctct ttttcgtcac ctacattcgg tttgttggaa ttaaaacttt
    84481 ccatatcaat tggatagtat tgctccaatt tatctttaaa atcttcttta ttatttcttt
    84541 tgacttcagt ttttttatca ggattaatca tgttccatag ctttacacca tcatctcctg
    84601 aaattgaaaa cacttgactt ttagtacctt catccgcttc atatcccata gctggaagct
    84661 ctgtccaatt atctctattg tagtaattta cagtatttac aaccataatg gcgcgtgcac
    84721 ccttatccat agctttttta aaagcatttt ttaaatcctt tgtataaatt ctatccatta
    84781 ctgcaatttt gcccctaaga tccaaaccta tcaaatcttg gtcttgcccc ttgcctatat
    84841 atacaaattt taatttacta ggagcttttg ttccatcttc atttgttgtg attttattct
    84901 tatcgaaaaa ggcccctata tttctgtatt taaaactttg tcctccaatg ttaaccttat
    84961 caaactcaac tgtttgattt ttagcagaag cgaccgctat cgcatcttca tgtgctgcag
    85021 ttcgtgttac atttccagtg tcggtcattt tcagatgatt atttgctact aaatcccatg
    85081 aagaacttga agcagaagtc gcatagttac ccgtagcgac aaccattgga atgcctgctt
    85141 ttcttaatgc cctaatagct tgccaatatt tctcacctac aagacctgtt cctgtaaaac
    85201 cagatgatac cgaaacaaca tcaacgttgt gtttgataga atcttcaata gcatgaaaca
    85261 ttgtttcatc acccgcaaac ccagatcctg cgtcagaata cattttataa gagaaaattt
    85321 gtgcattagg cgcaattcca tctatgccgt taaagttttt gatatcttgt tcagtatcat
    85381 ttccagcaag aatccctgca atatgcatcc catgtgggtc aaaataatcc cttccatcat
    85441 catatttttc tacagtgatt ttgccaccat tataataatt gaacgcatga gggattttat
    85501 cactcaacca ataattttta tcagttcctt ttaagtcttc ttttttaaat ctcattgagg
    85561 ctttggcatc atcatcgatt ctcatagcct tatgcctata atctgttcca gtatcgatat
    85621 ttgaaatgac catacctcta ccatcaaaat ttttcccaaa cggagcattg atagacttta
    85681 ggtaatcaat agcttcctca actccaattt cctttctggc atgattcatc atgggttgga
    85741 ctttttgtgc cctttcaacc gatgtaatac cttctatttg tttaatttta ttcagattat
    85801 cttgagttgt ttcaatagca ccaccgttaa aaattctatc ataagtatat aaaacttttg
    85861 tattcttaag actggatagc tccttgattg ctttttctcc agattcttta tctttaaatt
    85921 cagcaatata gacaacttta tcttcttttt tgggattttc tgtgttttta ttcacaaatg
    85981 agtccgtatg atctccttgg gatttattgg aattttcttc tttgattttt gcttcttcat
    86041 tgctagtttt agtgtctatc acaggatttt tactaacaac ttctttttcc tcaataattg
    86101 ttgttttctt ttcttcagta tcctgtgaag tttccatagc actatggata tcttcatgtt
    86161 tctctttatt ttctgctact ttttctttat cagagatgct taaagcatct tcagagctag
    86221 atgtgtctgc taagactacc tcattagggg cataggctgc taaaataact gcagctgtgg
    86281 ttaatgacaa tactgtactt tttttcattt taattcctta catatttata taacttccaa
    86341 tagataataa actttaactt tgctagcctt tgttataaaa agttttacta agtattatct
    86401 aggaaataga gtagtacatt tatatataat tgttatctct ccataaaaac agtatatcat
    86461 ttaaaaaaaa ttaattcaag aaaaattaat a
//
LOCUS       DBEROU010000003        72765 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.003, whole genome shotgun sequence.
ACCESSION   DBEROU010000003 DBEROU010000000
VERSION     DBEROU010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 72765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72765
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.003"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(391..1419)
                     /gene="queA"
                     /locus_tag="ACL8CL_000193"
     CDS             complement(391..1419)
                     /gene="queA"
                     /locus_tag="ACL8CL_000193"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HGS1329512.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            1570..2277
                     /locus_tag="ACL8CL_000194"
     CDS             1570..2277
                     /locus_tag="ACL8CL_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HGS1329513.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHVVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            2355..2864
                     /locus_tag="ACL8CL_000195"
     CDS             2355..2864
                     /locus_tag="ACL8CL_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329514.1"
                     /translation="MNKEEFLKIKEAYKSARTEERKSIIGFITKKKDKEGNFLFTKSK
                     DKPYTTRNQYSGGGGNKKYTSGSRLSRPYDLSNHMWIDLSYKGNDILISLQSFDIDPN
                     SKELHVLYDRIGILFEQSKKIPIFKDCYTITKVSDAFLKMETTNWELPLSEADMEEMV
                     NYIINHYEE"
     gene            2944..3120
                     /gene="rpsU"
                     /locus_tag="ACL8CL_000196"
     CDS             2944..3120
                     /gene="rpsU"
                     /locus_tag="ACL8CL_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HGS1329515.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            3250..4185
                     /gene="hprK"
                     /locus_tag="ACL8CL_000197"
     CDS             3250..4185
                     /gene="hprK"
                     /locus_tag="ACL8CL_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HGS1329516.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            4178..4966
                     /gene="lgt"
                     /locus_tag="ACL8CL_000198"
     CDS             4178..4966
                     /gene="lgt"
                     /locus_tag="ACL8CL_000198"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HGS1329517.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYRQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGFRVSQWLSVVLIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            4967..5393
                     /locus_tag="ACL8CL_000199"
                     /pseudo
     CDS             4967..5393
                     /locus_tag="ACL8CL_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895042.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
     gene            5366..5755
                     /locus_tag="ACL8CL_000200"
     CDS             5366..5755
                     /locus_tag="ACL8CL_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HGS1329518.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            5839..6969
                     /gene="hemW"
                     /locus_tag="ACL8CL_000201"
     CDS             5839..6969
                     /gene="hemW"
                     /locus_tag="ACL8CL_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HGS1329519.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            6974..7711
                     /locus_tag="ACL8CL_000202"
     CDS             6974..7711
                     /locus_tag="ACL8CL_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP thioesterase domain-containing protein"
                     /protein_id="HGS1329520.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVVRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            7722..8495
                     /locus_tag="ACL8CL_000203"
     CDS             7722..8495
                     /locus_tag="ACL8CL_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HGS1329521.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            8485..9102
                     /locus_tag="ACL8CL_000204"
     CDS             8485..9102
                     /locus_tag="ACL8CL_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HGS1329522.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLCLKSRRK"
     gene            9310..9711
                     /locus_tag="ACL8CL_000205"
     CDS             9310..9711
                     /locus_tag="ACL8CL_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HGS1329523.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            9711..9989
                     /locus_tag="ACL8CL_000206"
     CDS             9711..9989
                     /locus_tag="ACL8CL_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HGS1329524.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            9979..10752
                     /locus_tag="ACL8CL_000207"
     CDS             9979..10752
                     /locus_tag="ACL8CL_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HGS1329525.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRPLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            10753..12057
                     /locus_tag="ACL8CL_000208"
     CDS             10753..12057
                     /locus_tag="ACL8CL_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HGS1329526.1"
                     /translation="MKFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGRLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQIFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGET
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            12631..13509
                     /gene="pstS"
                     /locus_tag="ACL8CL_000209"
     CDS             12631..13509
                     /gene="pstS"
                     /locus_tag="ACL8CL_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HGS1329527.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            13509..14426
                     /gene="pstC"
                     /locus_tag="ACL8CL_000210"
     CDS             13509..14426
                     /gene="pstC"
                     /locus_tag="ACL8CL_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGS1329528.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            14416..15300
                     /gene="pstA"
                     /locus_tag="ACL8CL_000211"
     CDS             14416..15300
                     /gene="pstA"
                     /locus_tag="ACL8CL_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGS1329529.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            15311..16114
                     /gene="pstB"
                     /locus_tag="ACL8CL_000212"
     CDS             15311..16114
                     /gene="pstB"
                     /locus_tag="ACL8CL_000212"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS1329530.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            16127..16885
                     /gene="pstB"
                     /locus_tag="ACL8CL_000213"
     CDS             16127..16885
                     /gene="pstB"
                     /locus_tag="ACL8CL_000213"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS1329531.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            16897..17550
                     /gene="phoU"
                     /locus_tag="ACL8CL_000214"
     CDS             16897..17550
                     /gene="phoU"
                     /locus_tag="ACL8CL_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGS1329532.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            17707..18522
                     /locus_tag="ACL8CL_000215"
     CDS             17707..18522
                     /locus_tag="ACL8CL_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1329533.1"
                     /translation="MKSKKWIFVLCNFLASFFLVACQSGSNGSQSAVDAIKQKGKLVV
                     ATSPDYAPFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGK
                     ADLAVAGISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGT
                     VPESMVKEQLPKVQLTSLTNMGEAVNELQTGKIDAVHMDEPVALSYAAKNAGLAVATV
                     SLKMKDGDANAVALRKNSDDLKEVVDKVIQKLKDEGTYQSYLEKAASLTEVEE"
     gene            complement(18542..19813)
                     /locus_tag="ACL8CL_000216"
     CDS             complement(18542..19813)
                     /locus_tag="ACL8CL_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HGS1329534.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     RQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLEELEGHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEERVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCLLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            19955..20686
                     /gene="budA"
                     /locus_tag="ACL8CL_000217"
     CDS             19955..20686
                     /gene="budA"
                     /locus_tag="ACL8CL_000217"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047605 - acetolactate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0045151 - acetoin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HGS1329535.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELKERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            20696..21448
                     /locus_tag="ACL8CL_000218"
     CDS             20696..21448
                     /locus_tag="ACL8CL_000218"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HGS1329536.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDASIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSLFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            21560..22465
                     /gene="murB"
                     /locus_tag="ACL8CL_000219"
     CDS             21560..22465
                     /gene="murB"
                     /locus_tag="ACL8CL_000219"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HGS1329537.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            22625..23782
                     /locus_tag="ACL8CL_000220"
     CDS             22625..23782
                     /locus_tag="ACL8CL_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329538.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            23763..24569
                     /locus_tag="ACL8CL_000221"
     CDS             23763..24569
                     /locus_tag="ACL8CL_000221"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1329539.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWIMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDEMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            24566..25339
                     /locus_tag="ACL8CL_000222"
     CDS             24566..25339
                     /locus_tag="ACL8CL_000222"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1329540.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIITGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            25336..26406
                     /locus_tag="ACL8CL_000223"
     CDS             25336..26406
                     /locus_tag="ACL8CL_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1329541.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            26659..26898
                     /locus_tag="ACL8CL_000224"
     CDS             26659..26898
                     /locus_tag="ACL8CL_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329542.1"
                     /translation="MKMSTFFNKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            27173..27658
                     /locus_tag="ACL8CL_000225"
     CDS             27173..27658
                     /locus_tag="ACL8CL_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related/scramblase family protein"
                     /protein_id="HGS1329543.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            27680..30298
                     /gene="alaS"
                     /locus_tag="ACL8CL_000226"
     CDS             27680..30298
                     /gene="alaS"
                     /locus_tag="ACL8CL_000226"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HGS1329544.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGQ
                     DVFKLYDTYGFPVELTEEIAEEAGMTVDCEGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVGAVSEGTASLIFAETSFYAEMGGQVA
                     DYGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVDNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAIAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(30387..31841)
                     /locus_tag="ACL8CL_000227"
     CDS             complement(30387..31841)
                     /locus_tag="ACL8CL_000227"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HGS1329545.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNDFHWHWYHFT
                     GTDYDAKRRKSGIYLIQGDNKGWANEGLVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            32020..32769
                     /locus_tag="ACL8CL_000228"
     CDS             32020..32769
                     /locus_tag="ACL8CL_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329546.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLTSSDLETSLARLLNVFDFAENRYQVIETFSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPDQSLESIYLSLAGRKEEVSDASQGH"
     gene            32750..34384
                     /locus_tag="ACL8CL_000229"
     CDS             32750..34384
                     /locus_tag="ACL8CL_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329547.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLANLRKKQAKNTGKKVNVSARV
                     LSSYIFSSLLMLFMFINIAFRFPFEEMPSFFSTMVAILLVLAFSTSFTAFYNVFYESK
                     DLASYRPYAFKESEIIMAKGLSVLLPALAGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVTLVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFIFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIGLTLFLLYLTKKKVLPRFYD
                     VILLNSEDKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVVTVLFTSAFFPYL
                     MMIGLISKIRDSQIVPDIHPPYWLPLFFIALFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIYAVQSFLPVLTLLGLSLYLGLPIISMIYLIVAWILASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLST
                     ILAISLGVGALILLLALAIFGYHYYLSRILAEIEKR"
     gene            35068..36231
                     /locus_tag="ACL8CL_000230"
     CDS             35068..36231
                     /locus_tag="ACL8CL_000230"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HGS1329548.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTTYRLFNQ
                     EGDGFGGLTVDLYGNYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            36228..36905
                     /gene="aroD"
                     /locus_tag="ACL8CL_000231"
     CDS             36228..36905
                     /gene="aroD"
                     /locus_tag="ACL8CL_000231"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HGS1329549.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFAGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEVSAPGQISLASMKKI
                     REILDEA"
     gene            36895..37749
                     /locus_tag="ACL8CL_000232"
     CDS             36895..37749
                     /locus_tag="ACL8CL_000232"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HGS1329550.1"
                     /translation="MKLDGYTRLAAVVANPIKHSISPFIHNRAFEATATNGAYVAWEI
                     EASDLAETVANIRRYQMFGINLSMPYKEQVIPYLDELSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLLVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            37768..38835
                     /gene="aroB"
                     /locus_tag="ACL8CL_000233"
     CDS             37768..38835
                     /gene="aroB"
                     /locus_tag="ACL8CL_000233"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HGS1329551.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     VAEEKGLMPAGITQSITEMCQKFGLPVDYENWKVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSATIHPVSLEEMKDYLVK"
     gene            38845..40011
                     /gene="aroC"
                     /locus_tag="ACL8CL_000234"
     CDS             38845..40011
                     /gene="aroC"
                     /locus_tag="ACL8CL_000234"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HGS1329552.1"
                     /translation="MRYLTAGESHGPRLTAIIEGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIESDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            40021..41124
                     /locus_tag="ACL8CL_000235"
     CDS             40021..41124
                     /locus_tag="ACL8CL_000235"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HGS1329553.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQAMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVVK"
     gene            41135..41473
                     /locus_tag="ACL8CL_000236"
     CDS             41135..41473
                     /locus_tag="ACL8CL_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HGS1329554.1"
                     /translation="MSNIYDSANELSRGLRELPEYKAVKAAKDAIAADAEASKIFTDY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            41565..42848
                     /gene="aroA"
                     /locus_tag="ACL8CL_000237"
     CDS             41565..42848
                     /gene="aroA"
                     /locus_tag="ACL8CL_000237"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HGS1329555.1"
                     /translation="MKLKTNISHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVVTIQGVGMDGLKAPQNALDMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTIPLKKMGVSISGQTERDLPPLHLKGTKKLAPIQY
                     ELPIASAQVKSALMFAALQAQGESVIIEKECTRNHTEDMLQQFGAHLSVDGKKITVRG
                     LQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLIVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            42841..43317
                     /locus_tag="ACL8CL_000238"
     CDS             42841..43317
                     /locus_tag="ACL8CL_000238"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HGS1329556.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRHVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIAADKDNQRPLFLNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            43314..44162
                     /gene="pheA"
                     /locus_tag="ACL8CL_000239"
     CDS             43314..44162
                     /gene="pheA"
                     /locus_tag="ACL8CL_000239"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGS1329557.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            44159..45433
                     /gene="psr"
                     /locus_tag="ACL8CL_000240"
     CDS             44159..45433
                     /gene="psr"
                     /locus_tag="ACL8CL_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HGS1329558.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSFPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            45712..46557
                     /locus_tag="ACL8CL_000241"
     CDS             45712..46557
                     /locus_tag="ACL8CL_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS1329559.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDILPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            46547..47650
                     /locus_tag="ACL8CL_000242"
     CDS             46547..47650
                     /locus_tag="ACL8CL_000242"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS1329560.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLTRRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            47652..48638
                     /locus_tag="ACL8CL_000243"
     CDS             47652..48638
                     /locus_tag="ACL8CL_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1329561.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            48654..50087
                     /locus_tag="ACL8CL_000244"
     CDS             48654..50087
                     /locus_tag="ACL8CL_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HGS1329562.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGILARYFIARKKIKVANIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            50116..51150
                     /locus_tag="ACL8CL_000245"
     CDS             50116..51150
                     /locus_tag="ACL8CL_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HGS1329563.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVEP
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            51350..52087
                     /gene="mecA"
                     /locus_tag="ACL8CL_000246"
     CDS             51350..52087
                     /gene="mecA"
                     /locus_tag="ACL8CL_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HGS1329564.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVAFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            52238..53524
                     /locus_tag="ACL8CL_000247"
     CDS             52238..53524
                     /locus_tag="ACL8CL_000247"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HGS1329565.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGDKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            53526..54395
                     /gene="thrB"
                     /locus_tag="ACL8CL_000248"
     CDS             53526..54395
                     /gene="thrB"
                     /locus_tag="ACL8CL_000248"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HGS1329566.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            54471..54623
                     /locus_tag="ACL8CL_000249"
     CDS             54471..54623
                     /locus_tag="ACL8CL_000249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329567.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDDVSSRHCFEVVDRTDEVS
                     NIYTVR"
     gene            54690..55628
                     /gene="msrB"
                     /locus_tag="ACL8CL_000250"
     CDS             54690..55628
                     /gene="msrB"
                     /locus_tag="ACL8CL_000250"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HGS1329568.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLI
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            55686..57410
                     /locus_tag="ACL8CL_000251"
     CDS             55686..57410
                     /locus_tag="ACL8CL_000251"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329569.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLSKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFAESPENIHSELEQKQVTSGRVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQELWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYCEIN
                     ASQHGKED"
     gene            57412..59160
                     /locus_tag="ACL8CL_000252"
     CDS             57412..59160
                     /locus_tag="ACL8CL_000252"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329570.1"
                     /translation="MKRQTVNQTLKRLAVDLASHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGRVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFVDRQGSGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKRAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQFPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            59462..60721
                     /locus_tag="ACL8CL_000253"
     CDS             59462..60721
                     /locus_tag="ACL8CL_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HGS1329571.1"
                     /translation="MKVFQHVNIVTCDQNFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPSGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHVHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    60797..60932
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            60971..61471
                     /gene="rplJ"
                     /locus_tag="ACL8CL_000254"
     CDS             60971..61471
                     /gene="rplJ"
                     /locus_tag="ACL8CL_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HGS1329572.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            61547..61915
                     /gene="rplL"
                     /locus_tag="ACL8CL_000255"
     CDS             61547..61915
                     /gene="rplL"
                     /locus_tag="ACL8CL_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HGS1329573.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
     gene            62102..62272
                     /locus_tag="ACL8CL_000256"
     CDS             62102..62272
                     /locus_tag="ACL8CL_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329574.1"
                     /translation="MQKEGQSSRQFVKSHLMTIMGVDIVKATQLVDEMEQVGLIRFDE
                     FGNVGILVLEGQ"
     gene            62272..62520
                     /locus_tag="ACL8CL_000257"
                     /pseudo
     CDS             62272..62520
                     /locus_tag="ACL8CL_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
     gene            62534..62874
                     /locus_tag="ACL8CL_000258"
                     /pseudo
     CDS             62534..62874
                     /locus_tag="ACL8CL_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            62867..63247
                     /locus_tag="ACL8CL_000259"
     CDS             62867..63247
                     /locus_tag="ACL8CL_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGS1329575.1"
                     /translation="MIDLYLSKNRQRNQLLLDFFQNYGIEVSCHSVSEMTKDKLIEMM
                     SYSSDCFEFLSPNLLRFKNRDNLRLTDFVEIILKNPELTIRLPLAVSNKRVYPSLNLE
                     EARALLPRDTKQLIYMAQTHYLSN"
     gene            63260..63493
                     /locus_tag="ACL8CL_000260"
                     /pseudo
     CDS             63260..63493
                     /locus_tag="ACL8CL_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            63496..64083
                     /locus_tag="ACL8CL_000261"
     CDS             63496..64083
                     /locus_tag="ACL8CL_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1329576.1"
                     /translation="MKRIIPVYIFQQVNVLLVSLYLLKFLCIGELTILQILYGSSLIS
                     FLWMYGQRKQAHKVNMKSRMKWLGVEFVSLLIISLCFSLIHAQGSTNQANLIGLQHQI
                     PWFSFLLFLINASMVEEFLYREILWNLVRKLDIRVALTSVLFALAHHTGTILAWCLYV
                     SLGMFLGMVRYKSDLWGSMGLHLVWNLLVYSLLLF"
     gene            64285..64422
                     /locus_tag="ACL8CL_000262"
     CDS             64285..64422
                     /locus_tag="ACL8CL_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HGS1329577.1"
                     /translation="MAEEVLNLQLVSVQVDETDEVDGMRFSTFSTNRCGNWSAFSWEN
                     C"
     gene            64601..67210
                     /locus_tag="ACL8CL_000263"
     CDS             64601..67210
                     /locus_tag="ACL8CL_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HGS1329578.1"
                     /translation="MDYNFNLEHPFFFTNNDYSTDTSIKYQVSLPFNWHEVMNNDEWV
                     YQYPIGKFVERQGWKIHISSEYNSSHELLQDVAKICHEMRIPFKHLSTEDKFIMRNGK
                     LVSRGFSGKFITCYPNQNELESVLQRLESALKQYNGPYILSDKRWDEAPIYLRYGVFR
                     PSRDDEKKVAIDELIVGDEVVKDERLPVFKIPKGIVPPDFLNKWLDKKDKKQGDFPFI
                     IDNAIRFSNSGGIYNARLKEDGKKIILKEARPYTGLGFDGTYSSEKLASECKALKILN
                     EWSETPKIYWHGKIWEHTFLGIEHMKGVPLNRWVTNNFPLYEVVDKTKDYLLRVSKIV
                     EKLIDLTNKFHSENVYHQDLHLGNILVKDEDEISIIDWEQAVFSNDEKVVHKVAAPGF
                     RAWRETLPSEIDWYGIRQIAHYLYMPLVTTSDLTYNYVSQTRIEGKKLFESLGYTREH
                     IDYVESLLSYLDSKCPQIENISRKKVLKPMHEIRTIESEQDIQDFIIKLLRGFTLTYG
                     QWRKEFQSRFFPVHYYGLNFNQGIAFSDLAILWSYQQLAKKVKNFKFDDYYEIRTQVI
                     NEAVNNFKKSSLSGLFDGKIGTIWLIYEFGEIDRAVELFTTHFIEIFENSQNKNLYSG
                     QAGILLVGLYFLSKGEIDNKLGEEILIRLREYTLNYIENPETFCKVGASDVQSNDPYE
                     NFGGLLYGHAGVAWLFGEAYKLTGESIYKNGLELAVDKELVAYKVDSNNSLQYSQGHR
                     LLPYLATGSAGLLLLINRNKEILSSKYLKYLTSLERATDVVFCVLPGLFNGFCGLEVA
                     NNIYSDIDDNFSGQKKLIEQLYRYLCVIEEGFVIAGDNGLKITTDIASGFAGVAIGLV
                     SIMDNKLTILPQI"
     gene            67243..69183
                     /locus_tag="ACL8CL_000264"
     CDS             67243..69183
                     /locus_tag="ACL8CL_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HGS1329579.1"
                     /translation="MNQSNEIFLNTIQEKTHYKRSISSSFQELKKWNSLIFRRGRYWY
                     QFDNWSIPNTGVIKEWYVEDNDWIPKVSSSSLDNSNSLKIVFDVEKYTLPDDSILQDV
                     RISSDESYIVLVVSSLRSTNLIGIKKYTMEELFIIEDISVESSFYLGKFGVMYTRSKE
                     YGRPSKLFYKSFDSFTEEELFEENECSFRLKIVHIDSNNCFVKSVDFQKGRIFLYSFD
                     RTGFVRHSYTETVAPTPRDIALFSTEKAQYFLGLSSTEEKKDQLILRETSSGDRVSIT
                     IPYQDRARRVHCIGRYILLDCSNAANSVFYLATFKNNSLRELSVNKITFDEKTTLYEN
                     SFSDRVLLFLERRTFYEKILSFDLIENTLKTEFERPIIKSNNTKYFSKVIWTKKDSYD
                     VSIPISLFWKSEDTDELPRRKKCILSVYGAYGKNDNSDLDEIMLSIIDAGFIYAIVHV
                     RGGGYLGGEWYRSGKALNKWNSIRDFIEGVNYLRENDVIDSKRLGLITSSAGGIIAGA
                     VLNEEKNLLQSILLFSPFINPYDTLQNPNDPLSKTEIAEWGDIRDPEVKAYIKSYSPM
                     QNIEKARDSNTVIVNILGEKDPYINNNEVIEWSKKLNSIGVKSLLYLNKAAGHGGFTP
                     SDVLLMIDTLNYFFEEVGRNNL"
     gene            69180..70787
                     /locus_tag="ACL8CL_000265"
     CDS             69180..70787
                     /locus_tag="ACL8CL_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1329580.1"
                     /translation="MNYLKFIKKTKLILMGILIFLSSFNGVLLSGIIVYAGSLNQTSS
                     FSDVLRFGAISILGWSAIYISNYYLEVTEASITKDINVKIKQGYFREQYLSSEMVKDY
                     SSIISVLSNDLRLIEENYFRQIFEIISSILLFIVSLSFMLYLNFLVSIIFIVLSALPI
                     IVPVFMKKMLSNSANEYSNSNAEYTHIIKEIFNGFKTLKSYSVTKEIISLSDKKLDKL
                     EDSTFNLKRSEVLSKLVAVLISGFCFLVPLVVGCYFVIYHKSLSFSELIGIFLANDKV
                     LGPIQSIAYSLNKINTTKDLRKPFLKYLSGEKNFIDAEHDNNGLYTSSIDEIHMKDVV
                     YSITPENKLSIDFSFKSPFRVLLTGTSGSGKTTILNLINGSLKSQKGYVNLLSHGKKS
                     SDSIPTVDQTPYIFDTTIRENVTLFQNEYFSDDQIIEVLKKVNLYEELEKIDILNYQC
                     GENGSNLSGGQKQKIALARALIRNNKVYLFDEISANLDNDNSNSIHDILFNLGISFIE
                     VSHHYDLNDKRYTDIYKLENGTLFKIK"
     gene            70802..71542
                     /locus_tag="ACL8CL_000266"
     CDS             70802..71542
                     /locus_tag="ACL8CL_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329581.1"
                     /translation="MDKDYILKVKGLYHQFLLGNNKTLQVLKNVSLSASRGEFISILG
                     ISGSGKSTLLKCISSLLEPTSGEVILNGINPYKIRNAKLSSIRRNEVSFIFQAYNLIP
                     SLPVIENIALPLRLSQKKLTIKNVENLLKRMKFNAGLNDFVGTLSGGEQQKVAIARAV
                     IADSDIIFADEPTGALDSVSREVIFELLRELVGAGKCVIMVTHDIELASKTDRALILK
                     DGKIFKELHRPSGEELYKILEVQSTTEE"
     gene            71545..>72765
                     /locus_tag="ACL8CL_000267"
     CDS             71545..>72765
                     /locus_tag="ACL8CL_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329582.1"
                     /translation="MLRLVYYQFLHNKKQWLGVSPVIFVSSLVMGLAVNGVINVENNS
                     QVFVGLPDPKPIFMFPIVFGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASLLQLSF
                     LVGGQIFIISSIISFIAYLCSIFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVV
                     LISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGFSVIWLLSLQQIFQDST
                     ILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMFIVSKWNLLYRKPY
                     IKSISAAITGAILLISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIIS
                     IAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYY
                     SLEDILIDDMNLLGL"
BASE COUNT        21538 a        13079 c        16128 g        22020 t
ORIGIN      
        1 cgttgacgcg gtttgaagag atttttgaag agtataagtc tttgattttg agactaagga
       61 gagtcagggt caaagtagtg aatgattctg aaataactca taatagtctc ttgagaggac
      121 agataagagt aacaaatatg gtgctactca tgtgtcttgc attttgattt acaaacagcg
      181 aagaaaatat aggaagctga aataaaatat agatgaatca atttggagag tcaacgcaat
      241 ctatggcaat attttcgaag ctagggggaa gcattttaat ttcaatgtac taggcgtctt
      301 ctacagctag ctagagtttc aattgtagat gttctttgaa ataatatctt agcgatttat
      361 tggtattaga agaattagag aaattctttc tcaataaata aacatggcgt caccaaaact
      421 gaagaagcgg tagtgttctt ggatggcatg gtggtaggca tctaagacta attcacggcc
      481 tgcaaaggca gaaaccaaca tgaccagagt tgattttggc agatggaagt tggttgagaa
      541 ggcatccacg accttccact catacccagg tttgataaag atattggtcc aaccagaatc
      601 tgcttggatt tgcccatcaa acttggaacc aatagtttcc aaggtgcgga tagaagtggt
      661 tccgacagcg atgacgcgac caccattttc tttgacagag cgaagggtgg cagcagcttc
      721 ctcagaaagt tgatagaact ctgagtgcat ttcgtgttcg tccagattat ccacagaaac
      781 aggtctaaag gttccgagtc cgacatggag agtcagatag actagatgaa cacccttagc
      841 ttggatttct gccagcagtt ctttggtgaa gtgaagacca gcagtcggtg ctgcagcaga
      901 gccgctttcc ttggcgtaga cggtttgata acgttcacgg tcatctaatt tttcgtggat
      961 ataaggtggc agaggcattt ctcccagact ttccaagact tctaggaaaa ttccttggta
     1021 ttcaaagcgg acaatgcgtc ccccgtgggt caattcttct gtaacgacag cgctgaggcg
     1081 gccatcacca aagctgatac gagtaccgac cttgaggcgt ttggcaggtt tagccagaac
     1141 ttcccactcg tctccactag tgttcttaag gaggagaagt tccacatgac ctcctgtctc
     1201 cactttttga ccatagaggc gggcagggag aactcgggtg tcgttcatga caagggcatc
     1261 accaggttcc agcatatcaa taatagagtg gaaatgttta tcttgcattt ctcctgtctc
     1321 acggttgacg atgaggagtt tggaggcatc acgtttttca aggggcgttt gggcaatcaa
     1381 ttcctctggc aagtggaaat caaaatcagc tgtattcata tatttgttca ttctttctaa
     1441 gttctaatcc tatccattat aacacgtttc aagtttggac gctctttttt tagaaattaa
     1501 atcgttttca cttgacaaaa attggtctat accatataat aaatatagac agaaatggag
     1561 gatgaaaaga tgaaagttat taaagttgaa aaccaagttc aaggtggaaa agttgctttt
     1621 gagattttga aggaaaaatt ggctaatgga gctcaaactc taggacttgc gacaggaagt
     1681 agcccacttg aattttacaa ggaaattgtt gagagtgacc ttgatttttc aaatctaacc
     1741 agtgtcaacc ttgatgagta tgtgggcctt gatggggaca atccacagtc ttatcgttac
     1801 ttcatgcaag aaaacttgtt caaccaaaaa ccatttaaag aaagtttctt gcctcgtggt
     1861 gttaaggaca atgctgaagc tgaagttgaa cgctacaacc aaattttggc tgaccatcca
     1921 gttgacctcc aaatcttggg aatcggtcgc aatgggcata tcggctttaa tgagcctggt
     1981 actccatttg acagtcaaac gcatgtagta gaacttgacc agtctactat cgaagccaat
     2041 gcacgcttct ttgccaagat tgaagacgtc ccaacccaag ctatttcaat ggggattaaa
     2101 aacattttgg atgccaagtc tattattctt tttgcttacg gtgagtcgaa agcagaagcc
     2161 attgccggaa cagtatctgg cccagtgact gagaacctac ctgcaagtag cctccaaaat
     2221 caccctgatg taaccatcat cgcagatgct gaagcgctta gcttactcga aaaataaaaa
     2281 gtataagaac cgcttgctct ttggagtgag tggttttaga catataaaac ttcaacttaa
     2341 ctttaggagg acacatgaat aaagaagaat ttttaaaaat aaaagaagca tataagagtg
     2401 ctagaacgga agaaagaaaa agtataatag gcttcattac aaaaaagaaa gataaggagg
     2461 ggaattttct ttttactaag tcaaaagata aaccatatac cacaagaaat caatattcag
     2521 gaggcggagg aaataaaaag tacacctcgg gtagtagatt atctaggcca tatgatttat
     2581 caaatcacat gtggattgat ttgtcatata aaggaaatga catcttaatc tctttacagt
     2641 cttttgatat tgatccaaat agtaaagaac ttcacgtctt atatgataga ataggcatct
     2701 tatttgaaca atctaaaaaa attccaattt ttaaagattg ctatacaata actaaagtca
     2761 gtgatgcttt tcttaaaatg gaaacaacaa actgggaatt acctttatct gaagcagata
     2821 tggaggagat ggtgaactat attattaatc attatgaaga ataaaaaact tagtttcctc
     2881 ttgacaatta ttccttttac gtgtaaaata gaatagatct tgaacttgaa gggagtgaaa
     2941 aaaatgtcta aaacagtagt acgtaagaat gaatctcttg acgacgcact tcgccgtttc
     3001 aaacgtgcgg ttactaaagc tggtactctt caagaaacac gcaaacgtga attctatgaa
     3061 aaaccttctg taaaacgtaa acgtaaatca gaagtagctc gtaaacgtaa aaaattctaa
     3121 ttgaaatgaa aggctagaga aatctagtcc tttttctttt aaataaatac tccaaagcct
     3181 gcaaaaatct gaaacttcct cctacaattt gatataatag agagaagaac tcatttgaag
     3241 gaggaaatga tgtcggtttt agtaaaagaa gtgattgaaa agcttagact agacattgtc
     3301 tatggtgaac cagaattgct tgaaaaggaa atcaatacag cggatattac gcgacctggt
     3361 cttgaaatga caggctattt tgactactat acaccagagc ggattcaact tttggggatg
     3421 aaggagtggt cttatctgat cagcatgcct tccaacagcc gttatgaagt tttgaaaaaa
     3481 atgtttctac ctgagacacc agcagtcatt gttgcccgtg gtttggtggt tccagaggag
     3541 atgttaaagg ctgctagaga atgtaagatt gctattttaa ccagccgtgc agctaccagt
     3601 cgtttatctg gagagttatc tagctatctg gattctcgtt tggcagaacg taccagtgtg
     3661 cacggtgtct tgatggatat ttatgggatg ggcgtcttga ttcagggaga tagtggaatt
     3721 ggtaagagcg agacaggtct tgagcttgtc aaacgtggtc accgtttggt agccgatgac
     3781 cgtgtcgata tctttgccaa ggatgagatt actctctggg gtgaaccagc tgaaattttg
     3841 aaacacttga ttgaaattcg tggggttggt attatcgatg ttatgagtct ctacggtgcg
     3901 agtgctgtca aggattcttc acaggttcag cttgctgtct atttggaaaa ttacgatacg
     3961 cataagacct ttgatcgtct tggaaataat gcagaggaac ttgaagtttc tggcgtagcc
     4021 attcctcgta ttcgtattcc agttaaaaca ggtcgtaata tctctgttgt gattgaggca
     4081 gctgccatga attatcgtgc caaggaaatg ggctttgatg cgacccgttt gttcgacgaa
     4141 cgactgacaa gtctaatcgc tcgaaatgag gtgcaaaatg cttgatccaa ttgctattca
     4201 actaggaccc ctagccattc gttggtatgc cttatgtatt gtgacaggct tgattcttgc
     4261 ggtttatttg accatgaaag aagcacctag aaagaagatc ataccagacg atattttaga
     4321 ttttatctta gtagcctttc ccttggctat tttaggagct cgtctctact atgttatttt
     4381 ccgatttgat tactatagtc agaatttagg agagattttt gccatttgga atggtggttt
     4441 ggccatttac ggtggtttga taactggggc tcttgtgctc tatatctttg ctgaccgtaa
     4501 actcatcaat acttgggatt ttctagatat tgcggcgcct agcgttatga ttgctcaaag
     4561 tttggggcgt tggggtaatt tctttaacca agaagcttat ggtgcaacag tggataatct
     4621 ggattatcta cctggcttta tccgtgacca gatgtatatt gaggggagct accgtcaacc
     4681 gactttcctt tatgagtctc tatggaatct gcttggcttt gccttgattc tgatttttag
     4741 acggaaatgg aagagtctca gacgaggtca tatcacggcc ttttacttga tttggtatgg
     4801 tttcggtcgt atggttatcg aaggtatgcg aacagatagt ctcatgttct tcggctttcg
     4861 agtgtcccaa tggctgtcag ttgtccttat cggtctcggt ataatgatcg ttatttatca
     4921 aaatcgaaag aaggcccctt actatattac agaggaggaa aactaaatgt tagaagttgc
     4981 atatattctt gttgccctag ctttgattgt ctttttggtc tatctgatca ttactgtaca
     5041 aaagcttggt cgtgtcatcg atgaaacaga aaagacgatt aaaaccttga cttcagatgt
     5101 ggatgtgacc ttgcatcaca ccaatgagtt gttggctaag gtcaatgtct tggcagatga
     5161 tatcaatgtc aaggtggcta cgattgatcc actcttcagt gctgttgcag atttatctct
     5221 atctgtttca gacctcaatg accatgcgcg tgtcttgagc aagaaagctt catcagctgg
     5281 ttcaaaaaca ctcaagactg gtgcaagtct gtcagctctt cgtcttgcaa gtaaattttt
     5341 caaaaaataa aaaaggagaa tccttatggg taaattatcc tcaatccttt taggaaccgt
     5401 ttcaggtgca gctcttgcct tgtttttaac aagtgataag ggcaaacaag tttgcagtca
     5461 ggctcaagat tttctagatg atttgagaga agatccggag tatgccaagg agcaagtctg
     5521 tgaaaaactg acagaagtta aggagcaggc tacagatttt gttctgaaaa caaaagaaca
     5581 ggttgagtca ggtgaaatca ctgtggacag tatacttgct caagctaaat cctatgcttt
     5641 tcaagcgaca gaagcatcaa aaaatcaatt aaataatctc aaggaacaat ggcaagaaaa
     5701 agccgaagct cttgatgact cagaagagat tgtgattgat ataacagaag aataaaccat
     5761 caccatctcc gaacggacta tgtatctggg gatggtgatt tttatctgga atctagtctt
     5821 tgtggtataa taattactat gcagaaaaaa ccaacgtcag cctatgtgca catcccattt
     5881 tgtacccaga tttgttatta ttgtgatttt tcaaaggtct tcatcaaaaa tcagccagtc
     5941 gacagctatt tagagcatct gctggaagag tttcgttctt atgatattga aaagttgtca
     6001 accctttata tcggtggtgg aacaccgaca gccctgtcgg ctccgcaact ggaggtttta
     6061 ctgaatggct tgactaaaaa cttggacttg tctgtcttag aagagttgac cattgaagcc
     6121 aatccaggcg atttggatgc ggataagata gctgttttga aaaattcggc tgtcaatcgt
     6181 gtttcgctag gtgtccagac ctttgatgat aagatgttga aaaagattgg gcgcagtcat
     6241 ttggagaagg atatttatga aaatatcgat cgcctgaaac tggctggttt tgacaatatc
     6301 tccattgatt tgatttatgc tctgcctggt cagaccatgg agcaagtaaa ggaaaatgtg
     6361 gctaaagcca ttggattgga tattccccac atgagtttgt atagtttgat tttagaaaac
     6421 catacggtct ttatgaaccg gatgcgacgt gggaaattgc ctctgcctaa ggaggaacta
     6481 gaagcggaga tgtttgagta catcattgca gagctggagc gagcgggttt tgagcattat
     6541 gagatttcta atttctccaa acccggtttt gaaagtcgtc ataatctcat gtactgggac
     6601 aatgctgaat actatggtat tggtgcaggg gcatctggtt atgtcaatgg agtacgctat
     6661 aaaaatcatg gtcccattcg tcattatctc agtgcggttg aggaaggcaa tgcttgtatt
     6721 acagaagatc acctgagtca aaaggagcaa atggaagaag aaatgttctt gggactccgc
     6781 aagaaatccg gggtttccat ggcgcgattt gaggaaaaat ttggacagtc ttttgctgga
     6841 ctttatggag aaattgtcag agatttggtt caacaaggcc tcatgcagat tgagggtgac
     6901 cacgttcgca tgacaaagag aggtctcttt ttgggagata ctgtagcaga acgatttatt
     6961 ttggagtagg atcatgggct taacttatca aatgaaaatg aaaattcctt ttgatatggc
     7021 tgatatgaac ggtcatatca aacttccaga tgtgattttg ctgtcactgc aagtttcagg
     7081 gatgcagtcg attgaactgg gagttagtga taaggccatt ttggaagaat ataatctggt
     7141 ctggattatc gtagaatatg atattgaggt ggttcgtttg cctcgttttg cggaagaaat
     7201 taccatcgaa acggaagcct tgagctacaa tcgacttttt tgctaccgtc gctttactat
     7261 ttataatgaa gcggagcagg agctcatcca catgatggcg acctttgttc tcatggaccg
     7321 cgacagtcga aaagtccatg ttgttgaacc tgagattgtg gctccttacc agtctgattt
     7381 tgataaaaaa cttatccgtg gaccgaagta tgagtccttg gaagaaccga tcagcaagga
     7441 ttaccatgtt cgtttttacg acttggatat gaatggtcat gtcaataaca gtaaatactt
     7501 ggactggatt tttgaggtca tgggagcgga ttttttgacc cactatattc ccaagaaaat
     7561 caacctcaag tatgtcaagg aagttcgacc aggtggggtg attacatcgg ctgttgaacg
     7621 gactggactg gaaagcaagc atgaaattac aagtgatggg gttatcaatg cccaagcaat
     7681 cattacttgg caagaaataa aaaaggctta gggagaaata gatgaaatat aaaggctatt
     7741 taattgattt agacggaacc atttataagg ggaaagaccg aatccctgct ggagagactt
     7801 ttgttcatga attgcaaaag cgggacattc cctatctctt tgtaaccaac aatacaaccc
     7861 gcactccaga gagtgttaag gagatgttgg ctcagaattt taatatcgat acgcccctat
     7921 cgactgtcta cacagcgact ttggcaacca tcgactatat gaatgatttg gggcttgaaa
     7981 agaccgtcta tgtcgtcgga gaagcaggac tcaaggaagc catcaaggcg gctggttatg
     8041 tggaagacaa ggaaaaacct gcctacgtgg tagttggtct ggattggcaa gtcgactatg
     8101 aaaaatttgc cacagcaact cttgctattc aaaagggtgc ccattttatc ggaaccaatc
     8161 ctgacctcaa catcccgacg gaacgcggtc ttttgccagg tgctggctca ctgattacgc
     8221 tgcttgaagt agcgacacga gtgaagccgg tttatatcgg aaaaccaaat gccatcatta
     8281 tggacaaggc ggttgagcac ttaggtttgg aacgtgaaga gttaatcatg gttggggaca
     8341 attatttgac tgatattcgg gctgggattg acaatggcat tccaacgctc ttggtgacga
     8401 caggttttac taaggcagaa gaagtagctg gcctgccaat cgcaccgaca catgtggttt
     8461 ctagccttgc ggagtgggat tttgatgaaa actaaattga tcttttgggg ctctatgctc
     8521 tttctcctct ccctctccat ccttctgacc atttatctgg cttggatttt ctatcctatg
     8581 gagattcagt ggctaaactt aacgaatcga gtctatctaa aaccagaaac cattcaatac
     8641 aattttcata tcttgatgaa ttatctgacc aatcctttta gtcaggtctt acagatgcct
     8701 gattttcgtt cgtcagcagc tggtctgcac catttcgcag tggtcaagaa tctctttcat
     8761 ttggttcagc tagtagctct agtgacactg ccaagtttct atgtctttgt caataggatt
     8821 gtgaaaaagg actttttgtc tctttatcga aaaagtctcc tggctctagt agttttacct
     8881 gtaatgattg gacttggggg agttttgatt ggttttgacc aattctttac tcttttccat
     8941 caaattctct ttgtgggaga tgatacctgg ctttttgatc cagccaagga tcctgttatt
     9001 atgattttgc cagagacctt ctttcttcat gccttcctcc tcttttttgc cctctatgaa
     9061 aacttctttg gctatctgtg tctgaaaagt cgtaggaagt gaaaggaaag atgtttttaa
     9121 tttacatata taaatatata tcgtttaaaa ccatcgttaa gagtgaatta accaaatcca
     9181 attgtgtcaa tcgttcgcaa acagcctatt tttcctgaaa aaataggctt tttttctaaa
     9241 aatccctagt caagcgcctt atttttttgt ataatagaag tagcaaagta tagataagaa
     9301 gagaaaagca tgattacact atttctatca ccgagctgta catcatgtcg taaggctaag
     9361 gcctggttag aaaaacataa ggttcccttt gtggaacaca atattatgac cagtccttta
     9421 acaagaaaag aattgcaaca catcctttcc ttgaccgaaa atggtactga tgacatcatt
     9481 tcaactcgtt caaaaatttt tcaaaaattg aatattgatg tagagagtat ttcggtatcg
     9541 gaattgcttc atttgattga gcagtatcct agtcttttgc gtcgtccaat tattattgat
     9601 gccaagcgta tgcaaatcgg ttttaatgaa gatgagattc gtgcttttct ccctcgtagt
     9661 tatcgtaagc aagaactaaa agaagcaaga atgagagctg gtattagtta atgaacaaac
     9721 agtatagtta cccactagat ttgtcgtgga gcactgaaga acttgcttca gtgctttctt
     9781 tttttaatga tgttgaagct gcctatgaag gcaaagtaga ggctaaaaag ttactggact
     9841 cctataaggg attcaaagcg gtcgtgccaa gcaagagcga agagaaacgt ttgggacgag
     9901 aatttgaaac ggttagtggc tattcacttt accgagcagt tcaggctgcc aaagaaaaag
     9961 gggaaggaaa gatttctctt ggaaagtaaa tttgaatttg ctaaagagct tgttaagaaa
    10021 gcaggtcagt acattcttga ccatatgcag gaagacttgc gtgttgaaac caagtcctct
    10081 ccaacagatt tggtgaccag actggacaaa gaagttcagg aactcttggt tggtgagatt
    10141 ttgtcccgtt atcctgagga taagatttgt gctgaagaag gttgtctgcg agcctcggtt
    10201 caagagggca aggtttgggt cattgatccc attgatggta ccaataattt tgtagcccag
    10261 caggaagatt ttgctgttat gatggcttat tttgaaaatg gtcagggaca gtttggtctg
    10321 atttatgatg tggtcaaagg ggattgttac cacggtggtg gggaatttcc agtttgtcta
    10381 aatgataggc ccctagctcc ttttaaaact aaaccacttg gagattttct cattgcaggg
    10441 aatagtggta tgctggaaac caatgaatgg gggctggctg atttgagccg atcggttctt
    10501 ggtgttcgtg tctatggaag tgcggccatt agttttgcca agattttgtc agggcgtttg
    10561 ttgacctatc tcacttatct gcaaccatgg gattatgccg cagctagtat tttaggggaa
    10621 agtctgggct atcgggttgt gacccttttt ggtgaagctc ctgattttca aaccagacag
    10681 ccggtcatga tggtgcctct tgagatgcag gaggaaattc agtcctatat ttacgaaagg
    10741 aaaagaactt aaatgaaatt tccagaagga tttgttgaaa aatataaaga gatactagga
    10801 gatgaggcaa gagattttct tgcctctttt gaggaggaag cggtttcggc ctttcgggtc
    10861 aatcccttaa aagaagagca actttccttt tctgatgcca ttactcaaac cccttggggc
    10921 cactatggga aggtttcagg gaaatcgcct gagcatgcta caggtttagt ttattcgcaa
    10981 gaacctgctg ctcaaatggt ggctcaagta gcccaaccca gtcctggtat gaaggtcttg
    11041 gacttggctg ctgcaccggg gggcaaatca actcaactgg cagcctatct agcaggggag
    11101 gggctacttg tttccaatga aatttcaagc aaacgggcta agattttggt tgaaaacatg
    11161 gagcgttttg gagcgacaaa tgtcgtggtg accaatgaat ctgccgaccg cttggtcaag
    11221 gtctttaaag gctattttga cttaatcgtt cttgatgccc cttgctctgg tgaaggaatg
    11281 tttcgtaagc aacctgatgc tatggactat tggagcttag attatccgag tcaatgtgct
    11341 agcttgcaaa gagaaattct ggaggatgca gtgaccatgt tagctgaagg tggtcgtctg
    11401 gtttattcga cctgtacctg ggcacccgag gaaaacgaag agattgtcaa ttggttgctg
    11461 gaagagtatg attttgactt gttgccagta gagcatatca atggaatggt agctggtatt
    11521 gacctgccag aaacggctcg gatgtaccct catcagttta agggagaggg tcagtttgtt
    11581 gcccatctac agtttaaggg taacaatcca gctcctaaat ttaaggcaag taagagtaac
    11641 ctcagccgtg aacaagttgc cttgtggcag gaatttgccc aaaaccattt gaaggtcaat
    11701 ctaccgggta tcttgcagat ttttggagac cagctttatc tcttgccaga acttttgcca
    11761 gatttaggga agctcaagat tgctcgcaat ggactgcatc tgggtacttt taagaagaaa
    11821 cgctttgagc ccagtttcgc tcttggttta gccttgaaac cgagtcaggt cgaacagtcg
    11881 gttgaaattg gccaagaagc gtttgtcaag tatgcggctg gagaaaccgt tcagctggca
    11941 gaaagtctgc caaatggttg gtaccaagtt ttggttaaag gcaatggctt gggctttgca
    12001 aaggttactg gaaatgtttt gaaaaattat tttccaaaag gcctcagatt caaatgaaaa
    12061 tgctagtcaa atatcttatt ctatgttata gtgaaagtat ggattttttt agtaggatga
    12121 ttcttgatat cttgattgcc attacatgta gttttactac tcaatgaaaa tcaaagagca
    12181 aactaggaag ctagccatag atagctcaaa acattggttt gaagagattt tcaaagagta
    12241 caagaagttt agttattagc gttcttaccg cttgtaaact agatttctca taaaatagaa
    12301 tctttttctt ttagttgtaa actagtctgg gagagtagag aggtttgaga tacctttcta
    12361 gcttttggat tatcatctaa gaagagtaat ttcccttgca ttaaaaaggg gaaaaagaga
    12421 cacgaaatga ctataatggg tgacaatggg ggaagggata gacaagagat tttatccaca
    12481 tatgaaaaaa ggaggttagg aaagagtaat atagcctata ttatataaat aatcaattgc
    12541 gcagaaattt ggtaagaatt catgcgtcaa ctcataaaga actacttaaa aaattcacag
    12601 tattcataat tattttcgag gagaaaaaca gtgaaaaaaa gaaaaaagct tgctctgtct
    12661 cttatcgctt tttggctgac ggcttgttta gtaggctgtg ctagctggat tgatcgtgga
    12721 gaatccataa cggctgttgg ctcaactgcc ttgcaaccct tggttgaagt agcggcagat
    12781 gaatttggca cgatccatgt tggaaaaacg gtcaatgtcc aagggggagg ttctggtaca
    12841 ggcttgtccc aggttcagtc tggggcagtt gatataggaa actcagatgt atttgctgag
    12901 gaaaaagacg gaattgatgc ttctgctctt gttgaccaca aggtcgcggt agctggcttg
    12961 gctctgattg tcaataagga ggttgatgtt gataacctaa cgacagagca acttcgtcaa
    13021 atcttcatag gtgaggtaac caattggaaa gaggttggtg gtaaggactt acccatctct
    13081 gttatcaatc gggcagccgg ctctggctct cgtgctacct ttgatactgt cattatggaa
    13141 ggtcagtctg ccatgcaaag tcaggagcag gattcaaatg gagcggtaaa atcaatcgta
    13201 tcaaaaagtc caggagctat ctcttattta tctcttacct atatagatga ttcggtcaaa
    13261 agcatgaagt tgaatggcta tgacttaagt ccagaaaata taagtagtaa taattggccc
    13321 ttgtggtctt atgagcatat gtatacattg gggcagccca atgagttagc tgcagaattt
    13381 ctcaattttg ttctctcgga tgagacccaa gaagggattg tcaaaggatt gaagtatatt
    13441 ccgattaagg aaatgaaggt tgaaaaagat gctgccggaa ctgtgacagt gttggaaggg
    13501 agacaataat gaatcaagaa gaattagcta agaaaatgtt gcttccatca aagaattctc
    13561 gtctggagaa attaggaaaa ggtttgacct ttgcctgtct ttctttgata gtcatccttg
    13621 tggccatgat tttggttttc gtagcgcaaa aaggcttgtc gaccttcttt gtcaatggtg
    13681 tgaatatctt tgactttctt ttgggaggaa cttggaatcc ttctagtaaa gaatttggtg
    13741 cccttcctat gattttgggt tcctttatcg ttaccattct ctcagccctt atcgcaacac
    13801 cctttgctat tggtgcagca gtttttatga ccgaagtatc accaaaaggg gcgaagattt
    13861 tgcaaccagc tattgaactc ctggttggga ttccttcagt agtgtacgga tttattggct
    13921 tgcaagtcgt cgttcccttt gttcgcagtg tctttggtgg gactggtttt gggattttgt
    13981 cagggatttc cgtcctcttt gtcatgattt tgccgaccgt aacctttatg acaacggata
    14041 gcttgcgtgc ggttcctcgt tattatcgtg aagccagttt cgctatggga gccactcgct
    14101 ggcagactat ctggcgtgtg atcttgaagg cggcccgttc tggtattttc actgcagtgg
    14161 tctttgggat ggcgcgtgcc tttggtgagg ctctagctat ccagatggtt gtcggaaact
    14221 cagctgttat cccaacttcc ttgaccacac cagctgcaac tttaacttct atattaacta
    14281 tgggaattgg gaacactgtc atgggaactg taaataataa tgttctctgg tcactggcct
    14341 tggtactgct cttgatgagc ttagttttta acagtgtgat taaattgatt acgaaagaaa
    14401 gaggaaagaa aaattatgcg cgctaagaaa ttagataaac ttgcaacagc tgtcctctat
    14461 acgattgcta gcatcattgt gacaatcttg gcttccttga ttctctatat cttggttcgg
    14521 ggcttgcccc atatctcttg gtctttcttg actggaaggt cttctgcttt tcaagcaggt
    14581 ggtgggattg gcattcagct ttacaattcc tttttcctat tggtcattac cttgattatt
    14641 tctgtacctc tttctatggg agctgggatt tacttggctg aatatgctaa aaaaggtcct
    14701 gttaccaact ttgtgcggac ttgtattgaa atcttgtcct ctttaccatc agtggtggtg
    14761 ggtctctttg gttacttgat ctttgtagtc cagtttgagt atggattttc aatcatttca
    14821 ggtgccttgg ccttgacagt ctttaacttg cctcagatga cgcgtaatgt agaggatagt
    14881 ttgaaacacg ttcaccatac ccaacgtgag gctggtctgg ctcttgggat ttctcgctgg
    14941 gagacagtgg ttcatgttgt tattccagaa gcgcttccag gtattgtaac gggtgtcgtc
    15001 ttggcatctg gtcgtatctt tggcgaagct gcagctctga tctatacagc agggcaatcg
    15061 gcgccagctc ttgactggtc taactggaat atcctcagtg tgactagccc catctctatc
    15121 ttccgtcaag cagaaacctt ggctgtccat atctggaaag tcaatagtga aggcactatt
    15181 ccagatggaa ccattgtatc agcaggttct gccgctgtgc tcctgatctt tatcctgatt
    15241 tttaactttg gagctcgtaa gttcggaagc tatctacaca agaaattaac cgctgcctaa
    15301 aggagaagcc atgtcaacat ataactggga tgagaagcat atccttacct ttcctgaaga
    15361 aaaagtagcc ctttctacta aggatgtcca tgtttactat ggtaaaaatg aatccattaa
    15421 ggggattgat atgcaatttg aaagaaataa aattacagct ttgattggcc cgtcgggatc
    15481 ggggaaatct acctacttac gcagtctcaa tcgcatgaat gatactattg atattgctaa
    15541 agtaactggg cagattctct atcgtggaat tgatgtcaac cgtccagaaa tcaacgttta
    15601 tgaaatgcgt aaacacattg gaatggtttt tcaacgcccc aatccatttg ctaagtcaat
    15661 ttaccgtaat attacctttg cgcatgaacg tgctggagtt aaggataagc aagtcctaga
    15721 tgaaatcgta gaaacctccc ttcgtcaggc tgccctctgg gatcaggtta aagacgatct
    15781 ccacaagtca gccttgacct tatcaggtgg tcagcaacaa cgtctctgta tcgctcgtgc
    15841 catctctgtt aagccagata tcctcttaat ggatgagcca gcctcagcct tggatccgat
    15901 tgcgaccatg caactagaag agaccatgtt tgagctcaag aaaaacttta ccatcatcat
    15961 tgtaacgcat aatatgcagc aggctgctcg tgcaagtgac tatacaggct tcttttactt
    16021 gggtgatttg attgagtatg acaagactgc aactattttc caaaatgcca agctacagtc
    16081 caccaatgac tatgtatctg gtcactttgg ttagaaagga aaccgtatga cagatgcgat
    16141 tttacaggta tcagacctgt ccgtttatta taataaaaag aaggctttga atagtgtttc
    16201 cctatctttc caacctaagg aaattacagc cttgattggt ccatctggat cagggaagtc
    16261 aaccctcctc aagtctctca accgcatggg agatctcaat ccagaggtga ccacaactgg
    16321 atccgtggtg tacaatggtc acaacatcta cagtccgcgt acagatacgg ttgaattacg
    16381 taaggaaatc ggaatggttt tccaacaacc taatcctttc cctatgacta tctatgagaa
    16441 tgttgtctac gggcttcgta tcaatggaat taaggataag caggttctgg atgaagccgt
    16501 agaaaaagcc ttgcaaggtg cctctatctg ggatgaggtc aaggatcgtc tatatgattc
    16561 agctattgga ttgtcaggtg gtcaacagca gcgtgtctgc gtggcccgtg tcttggcaac
    16621 tagtcctaaa atcatcctct tggatgagcc aacttcggct ttggatccga tttcagctgg
    16681 taaaattgag gaaaccttgt atggtctaaa agacaagtac accatgcttc tggtaacccg
    16741 ttccatgcag caagcttcac gtatctctga taagacagga tttttcctag atggagattt
    16801 gattgaattt aatgatacca agcagatgtt ccttgatccc caacacaagg aaacggaaga
    16861 ctatattaca ggaaaatttg gataaggaga tgaaagatgt tacgatctca atttgaagaa
    16921 gatttagaga aattacataa ccagttctac gctatgggac aagaagtgct ctcacaaatc
    16981 aatcgtacgg tacgtgcttt tgtcacgcat gaccgtgacc tggcaaaaga ggtcatcgaa
    17041 gatgatgcag aagtaaatga atacgaagtg aaactggaaa agaaatcatt tgaaatgatc
    17101 gcactccaac aaccagtctc tcaagatttg cgtacagtct tgactgtcct taaggctgta
    17161 tcagatgtgg agcgtatggg ggatcacgct gtagccattg cccaggcaac catccgtatg
    17221 aagggggaag agcgcattcc agctgtagag gaagaaatta aaaaaatggg acgtgaagtt
    17281 aaaagcgttg ttgaagcagc acttgatctt tatcttaatg gttctgttga cgacgcatac
    17341 cgggtggcct ccatggatga gcaaattaac cactattttg aaactatccg tgaccttgcg
    17401 actgaagaga ttaagaaaaa tccagaagcc attgttacgg gtcgtgatta tttccaagtt
    17461 atttcctact tggagcgtat cggtgactat gctaaaaata tctgtgaatg ggttgtctac
    17521 tttgaaacag gtaagattgt cgaactataa aatagtttaa ttctgtataa aaaggagctt
    17581 gaaatcaact gatggctcct ttttttgaac gaaaaaatat ttttgagata aaaatttaaa
    17641 aaacacttga caagtaactt ggatatcgtt ataattatac aaaattaaca gagaggttgt
    17701 ttatttatga aatcaaaaaa atggatattt gttttatgta attttcttgc aagtttcttc
    17761 ttagtagctt gtcagtcagg ttctaatggt tctcagtctg ctgtggatgc tatcaaacaa
    17821 aaagggaaat tagttgtggc aaccagtcct gactatgcac cctttgaatt tcaatcattg
    17881 gttgatggaa agaaccaggt agtcggtgca gacatcgaca tggctcaggc tatcgctgat
    17941 gaacttgggg ttaagttgga aatctcaagc atgagttttg acaatgtttt gaccagtctt
    18001 caaactggta aggctgacct agcagttgca ggaattagtg ctactgacga gagaaaagaa
    18061 gtctttgatt tttcaatccc atactatgaa aacaagatta gtttcttggt tcgtaaggct
    18121 gatgtggaaa aatacaagga tttaactagc ctagaaagtg ctaatattgc agcccaaaaa
    18181 gggactgttc cagaatcaat ggtcaaggaa caattgccaa aagttcaatt aacttcccta
    18241 actaatatgg gtgaagcagt caatgaattg cagactggaa aaatagatgc tgttcatatg
    18301 gatgagcctg ttgcacttag ttatgctgct aaaaacgctg gcttagctgt cgcaactgtc
    18361 agcttgaaga tgaaggacgg cgacgccaat gccgttgctc ttagaaaaaa tagtgatgat
    18421 ttgaaagaag tggtagacaa ggtcatccaa aaacttaagg atgaaggaac ttaccaaagc
    18481 tatcttgaaa aagcagcaag tctaactgaa gttgaagaat aagaaaaagc agagttggat
    18541 gtcattccaa ttctgctttt aaatagttta aaacattttc cagattgtct agggacacct
    18601 tggcaaattg ataaggacag gtacttaaat aggcctcttc aaagaagtcc aagcccggtt
    18661 gactgtgttt gagactggcg attttaggat actgtctcag gtctagcaat aaaccatcgt
    18721 gtaggggata atgaggaagg agacaaggtg ttttagttat cctttcctcg atttgttttt
    18781 ggtaagattc atgattggtc aagcgagcca aacgattttt ttcaaacaat tgactgtcaa
    18841 tatagagtga cagggccttt tgcataatga ggttgctgtc gtagtctagg atatttttgt
    18901 aggccacaaa tgcttctttg atagcatttg gaagaatgag tgctgtaata cgaagggcag
    18961 gaaaaaggct ggtcgagaag gacttgatat aaatgacacg ctcctctgta tcaagatagt
    19021 ggaaggtttg gcccttcttg gagtccaaat cacccagata atcgtcctct acgatataga
    19081 catcatactt ggcagctaag ttaagaatag atcgtttgtc ctgctcagaa taggaatgtc
    19141 ccagaggata gtgaaatcta ggaatggtgt agaaaaactt aatttttcct gttttgaagt
    19201 ggccttccag ctcctccaag tcaatcccat caatgcctcg ttcaatcgtt tgatagtcca
    19261 gcccctgtgc aatcaagagg cgattcattc gatggtaggt tggctgttcc accaagattt
    19321 ccttggcttg tctaggaaag gatatttgag agaggataaa caaggcttgt tgggttccag
    19381 aagtcagtac tagttggtta gccttgcagt agagagcttg ctcaaagagg agtttgtgaa
    19441 tggactgtct taggtcttct aatccttctt gattgtcata gtagttgaag aggtagtttt
    19501 ctcggccaat caaggtttca ttgacacaga gtcggaaatc gtcataggca ctggcatgtt
    19561 cgtcggtaac ctcgatttct aggtcttgat gttgcccttg ttctaataca tagtagccac
    19621 tctgaggctt ggcatagagg tattgttcgt gccgtaattc cagcagggct cgttgaatgg
    19681 tgtccttgct gcagtgaaag tcaaggctca gttgacggat agaaggcagg cgactacccg
    19741 tcggaaatcg tccagactcg ataccatttt tcagatagga aacgacctct ttgtacttgc
    19801 tttgtttctt cattccagac ctcccttgat tttgttacat tgtacccttt tttttccttc
    19861 ttggcaatgt ctagagcgtt tctctcgttc acatctaggg gcaaatgtgg tatacttagg
    19921 agtaagattt atagaataac agacaagaaa gtgaatggat aagaacgtgc aggaaccagt
    19981 gaaattattt caatacaata ctcttggagc cttgatggct ggcctttatg gtggtactat
    20041 gacagtagga gaattgctag aacatggtga ccttggtttg ggaaccttgg attccattga
    20101 tggggaattg attgtcttgg atggcaaggc ttatcaggcc aaagggtcag gagaccagcc
    20161 agaaattgtg gaagtgtcac cagatgccct tattccttac gctgcagtgg taccgcatca
    20221 ggcagaggtc attttccgcc agcgctttga gatgacagac aaggaattga aagaacgaat
    20281 tgagtcttat tatgatgggg aaaatctttt ccgctctatc aagattcggg gggaattttt
    20341 acatatgcat gtgcgtatga ttcctaagtc aacacccgat accaagtttg ctgatgtcgc
    20401 aacccatcaa ccggaatata gtcgtgacaa tgttgcgggg actattgttg gtttctggac
    20461 gcctgagatt ttccatgggg tcagtgtggc aggctaccat ctgcacttca tatcagatga
    20521 tttgaccttc ggtggacatg tcatggattt tgtcatcaag gaaggcatta tcgaggtggg
    20581 agcagttgac cagttggacc aacgtttccc agtccaagac cgtcaatact tgtttgctaa
    20641 gttcaatgtt gacgagatga aaaaagatat tgaaaaggca gaataggaga agaaaatgac
    20701 cattcatatc attattacca tgttgctgtt gctagctttt ctgataggaa gcatttggtt
    20761 tgccaaaaag aaataccaga ttaatctagc tgtcttgggc ttgggtgcag tcgctttctt
    20821 tgtctcttcg cagattttgg aaaaactggt gcatatcttg attttgcacc ctcaaaaaga
    20881 cgctagtatt gccctcttgc aagatcatcc gcttatctat attatctatg gcctagccat
    20941 ggcagcattt tttgaggaaa cggctcgtct tgttttcttc aaatggttgg agaaaaagag
    21001 aagtttggaa aaggcagatg ccttggccta tggcctaggg catggtggct tggagttgat
    21061 ttttctaggc cttattagtt tactcaatct ttacatcgtt ctttcggcag ttcaaactca
    21121 gaatccacag gttatgcaat tactgtctga aaatatgtta aaaaccattc agtcactttc
    21181 ggtctggcag atttatttgc ttggttttga gcgaatcttg gcgcttggtt tccaattact
    21241 tttgacagtt tgggtttacc aagctgttcg ccagaagaaa tggatttatc tcctagcagc
    21301 ctatggcttg catgccttct ttgatctggc accatctctt ttccaagtag gctggttgac
    21361 aaatccagtc ttggttgaag tgattctagc actggagctc gttctggtcg cctatggaac
    21421 caaggaaatc ttttgtaaaa aatcataaaa aggggggaaa ctctttttct tatgcaaaat
    21481 ccaaacaagg tatttttatg gtcgtcaaat gtctctaaaa atggtataat ggaatgaatt
    21541 ttgtaaaagg aagagtgaca tgtctgtaag agaaaaaatg cttgaaatct tagaaggaat
    21601 tgatatccgt tttaaggaac ccttgcatag ctatagttat acaaaagtag gtggagaggc
    21661 tgattatttg gtctttccac gaaatcgttt tgagttggcg cgccttgtga aatttgccaa
    21721 ccaagaaaat atcccttgga tggttcttgg caatgcaagc aatatcatcg ttcgtgatgg
    21781 tgggattcgt ggatttgtca tcttgtgtga caagctcaat aacgtttctg ttgatggcta
    21841 taccattgaa gcagaagctg gggctaactt gattgaaaca actcgcattg ccctccgtca
    21901 tagtttgact ggctttgagt ttgcttgtgg tattccagga agcgttggcg gtgctgtctt
    21961 tatgaatgcg ggtgcctatg gtggcgagat tgctcacatc ttgcagtctt gtaaggtctt
    22021 gaccaaggat ggagaaatcg aaaccctgtc tgctaaagac ttggcttttg gttaccgcca
    22081 ttcagctatt caggagtctg gtgcagttgt cttgtcagtt aaatttgccc tagccccagg
    22141 aacccatcag gttatcaagc aggaaatgga ccgcttgacg cacctacgtg aactcaagca
    22201 acctttggaa tacccatctt gtggctcggt ctttaagcgt ccagtcgggc attttgcagg
    22261 tcagttaatt tcagaagctg gcttgaaagg ctatcgtatc ggtggcgtag aagtgtcaga
    22321 aaagcatgca ggatttatga tcaatgtcgc agatggaacg gccaaagact acgaggactt
    22381 gatccaatcg gttatcgaaa aagtcaagga acactcaggt attacgcttg aaagagaagt
    22441 ccggatcttg ggtgaaagca agtaggaagc tgtatttgaa aagctgctgc tatgtcatgg
    22501 aaaggggatg aaagcctatc ggtagcgaag atgtatgcag gtggttttac tccctgcaag
    22561 aggtagtggg gacctgacag agccccgatc ggttaatcta tgaaaaagaa ggaatttatg
    22621 ccaattgaaa aaaccaatta tcgaattcaa aaacgtctct aaagtttttg aagacagcaa
    22681 caccaaggtt ctcaaagaca tcaactttga gttggaagaa gggaaattct acacccttct
    22741 aggtgcatct ggttcgggga aatcaactat cctaaacatt attgcaggtt tactggatgc
    22801 gacgacagga gatatcatgc tagacggtgt tcgtatcaat gatattccaa ccaacaagcg
    22861 cgacgtacat accgtcttcc aatcctatgc cttgttccca catatgaatg tgtttgaaaa
    22921 tgttgccttt ccacttcgct tgcgtaaaat tgataagaaa gaaatcgagc agcgtgtagc
    22981 ggaagttctc aagatggttc agttggaagg ttatgaaaaa cgttccatcc gcaaactttc
    23041 tggaggacaa cgtcagcgtg tggccatcgc ccgtgctatc atcaaccaac cccgtgtggt
    23101 cttgttggac gagcctttat cagcgctgga cttgaaattg agaacagaca tgcagtacga
    23161 attgcgtgaa ttacaacaac gattgggcat tacctttgtc tttgtcactc acgatcagga
    23221 agaagctctt gccatgagtg actggatttt cgttatgaat gatggcgaga ttgtccagtc
    23281 tggaacccct gtggacatct acgatgagcc aatcaaccac tttgttgcca cctttatcgg
    23341 ggagtcaaac atcttgccag gtaccatgat tgaggactac ttggtcgaat ttaacggcaa
    23401 acgctttgaa gcggttgatg gtgggatgaa gccaaatgaa cctgttgagg tcgttattcg
    23461 tccagaggac ttgcgcatta cccttcctga agaaggcaag ctccaagtta aggtcgacac
    23521 ccagctcttc cgtggagttc attatgaaat tatcgcctat gacgaacttg gaaatgaatg
    23581 gatgatccac tcaacccgta aggccatcgt gggtgaggaa atcggtctgg actttgaatc
    23641 agaagacatc cacatcatgc gtctcaatga aaccgaagaa gagttcgatg ctcgtatcga
    23701 agaatacgtg gaaatcgaag agcaagaagc aggtttgatc aatgcaatcg aggaggaaag
    23761 agatgaagaa aacaagctct aaactctttg tagtacccta catgctttgg attgcgctct
    23821 ttgtattggc acccttggtc ttgattttcg gtcaatcctt tttcaacatc gaaggccagt
    23881 tcagtttaga aaattacaaa tcttactttg cgtcacaaaa cttgacctat cttaaaatga
    23941 gtttcaactc agtgctttat gcaggcattg tgacctttgt gacactgctt atcagttatc
    24001 cgacggccct ctttttgacc cgtctcaaac accgtcaact ctggattatg ctgattattc
    24061 tgccaacttg gatcaacctt cttcttaagg cttatgcctt tatcgggatt tttggtcaga
    24121 atggctctat taaccaattc ttggaattta tcggaattgg ttcacagcag ttgctattta
    24181 ctgatttctc atttatcttt gtcgcaagct acatcgagct cccctttatg attttgccga
    24241 ttttcaatgt cttagacgaa atggataata atctcatcaa tgctagttac gaccttggtg
    24301 caactaagtg ggagaccttc cgtcatgtca tcttccctct atctatgaac ggtgtgcgaa
    24361 gtggggttca gtcggtcttt atcccaagtt tgagtctctt catgctgacc cgtttgattg
    24421 gtgggaaccg cgttatcacc ttggggacgg ctattgagca gaattttcta accaatgaca
    24481 actatggtat gggttcaact atcggtgtga ttctcatcct gaccatgttc atcaccatgt
    24541 gggtgactaa ggaaaggaga gaacgatgaa aaaatttgcc aacctttatc tgggactggt
    24601 ctttctggtc ctctacctgc cgatttttta cttgattggc tatgccttta atgctggtga
    24661 tgatatgaat agctttacag gttttagctg gactcacttt gaaaccatgt ttggagatgg
    24721 gagactcatg ctgattttgg ctcagacatt tttcttggcc ttcctatcag ccttgatagc
    24781 gaccattatc gggacttttg gtgccattta catctaccag tctcgtaaga aataccaaga
    24841 agcctttcta tcactcaata atatcctcat ggttgcgcct gacgttatga ttggtgctag
    24901 cttcttgatt ctctttaccc aactcaagtt ttcacttggc tttttgaccg ttctatctag
    24961 tcacgtggcc ttctccattc ctatcgtggt cttgatggtc ttgcctcgac tcaaggaaat
    25021 gaatggcgac atgattcatg cggcctatga cttgggagct agtcaatttc agatgttcaa
    25081 ggaaatcatg cttccttacc tgactccgtc tatcattact ggttatttca tggccttcac
    25141 ctattcgtta gatgactttg ccgtgacctt ctttgtaaca ggaaatggct tttcaaccct
    25201 atcagtcgag atttactctc gtgctcgcaa ggggatttcc ttagaaatca atgccctgtc
    25261 tgctctagtc tttctcttta gtattatcct agttgtaggt tattacttta tctctcgtga
    25321 gaaggaggag caagcatgaa aaaaatctat tcatttttag caggaattgc agcgattatc
    25381 cttgtcttgt ggggaattgc gactcattta gatagtaaaa tcaatagtcg agatagtcaa
    25441 aaattggtta tctataactg gggagactat atcgatcctg aactcttgac tcagtttaca
    25501 gaagaaacag gaattcaagt tcagtacgag acttttgact ccaacgaagc catgtacact
    25561 aagataaagc agggtggaac gacctacgat attgccattc caagtgaata catgattaac
    25621 aagatgaagg acgaagacct cttggttccg cttgattatt caaaaattga aggaatcgaa
    25681 aatatcggac cagagtttct caaccagtcc tttgacccag gtaataaatt ctccatccct
    25741 tacttctggg gaaccttagg aattgtctac aacgaaacca tggtagatga agcgcctgag
    25801 cattgggatg acctttggaa gccggagtat aagaattcta tcatgctctt tgatggggcg
    25861 cgtgaggtgc tgggactagg actcaattcc ctcggctaca gcctcaactc caaggatctg
    25921 cagcagttgg aagagacagt ggataagctc tacaaactga ctccaaatat caaggctatc
    25981 gttgcggacg agatgaaggg ctatatgatt cagaataatg ttgcaatcgg cgtgaccttc
    26041 tctggtgaag ccagccaaat gttagaaaaa aatgaaaatc tacgttatgt ggtaccgaca
    26101 gaggccagca atctttggtt tgacaatatg gtcattccca aaacagttaa aaaccaaaac
    26161 tcagcctatg cctttatcaa ctttatgttg aaacctgaaa atgctctcca aaatgcggag
    26221 tatgtcggct attcaacacc aaacctacca gcgaaggaat tgctcccaga ggaaacaaag
    26281 gaagataagg ccttctatcc cgatgttgaa accatgaaac acctagaagt ttatgagaaa
    26341 tttgaccata aatggacagg gaaatatagc gacctcttcc tacagtttaa aatgtatcgg
    26401 aagtaggaga taaatgtaag aagacgaatc agtcaagctg gttcgttttt aaggaataga
    26461 agtcagtttt tagagcccta tctttttcta ttctagttgt ttatttgctt atttgcttgc
    26521 ctagtagtat actaagccta tactcttcaa accacgtcag cgttgccttg ccgtactcaa
    26581 gtacagcttg aggctagctt cctagtttgc tttttgattt ttattgagta ttaaccttac
    26641 agaaagcggc aacaaataat gaaaatgtct acttttttca acaaaagttt ctggccaacc
    26701 tttatgattg ttaaccaaac tgctatacta ttccatttaa aggatggctt ggatcgacaa
    26761 tacctaacaa ctgagtccat atattgggtg ataggcacct ttatatttgg aaatattcta
    26821 gtagctgtgt ttagtaatat gaaaatttgg gataaaaaga aaaacggcag taagaaaaaa
    26881 tatatcctga aaaagtaatc agagatgtag ggaattggtc taattcaacg gagatcattt
    26941 ccagttgacg caagattaaa aaaacgatat aataagaaag ttgagaattg attttcactt
    27001 gtcttagtcc agagaaatgg cggtgctgcg agccatctaa gctaggaagt catgctactc
    27061 aatcatacaa ttgcataaga acaagagaat gacaagttca ttgaatgaag gtggtaccgc
    27121 ggtttttcgc ccttcgtgat atgagcttgt cttttgattt ttggaggtgt tgatgaaaac
    27181 atttcttgtc aaacaaaagt ttcgtcttgg aggcgaacgc ttcgctatca aggatgacag
    27241 gggagaaatt gcctatcagg tggagggatc attttttaag attcccaaaa cttttaccat
    27301 ctatgatgcg gctggtgaac aagtcagtca gatcagtaaa gaaatcttga ccttgcttcc
    27361 tcgttttgag attcagcttc gggatggctc gagttttgtc attcgtaaga agttgacctt
    27421 ttggcgagat aagtatgagt ttgataatct aggtcttcgt atcgagggca atatctggga
    27481 tttgaatttc aaattgctgg atgatcgcga tcagttgatt gcggaaatta agaaggaact
    27541 cttccatctg acctctacct ataccgtaac ggttcttgag gacgcttatg cagacctagt
    27601 catttccctc tgtgtcgcga ttgactatgt ggaaatgctg gaaagccaat cacattaaac
    27661 aagtgaataa ggagacaata tgaaacaact atctagtgca caagtacgcc aaatgtggct
    27721 tgatttctgg gcgaccaaag gtcactcagt agaaccatca gtaagtttgg ttcctgtaaa
    27781 tgacccaact cttttgtgga tcaactctgg ggtagcaacc cttaagaaat actttgacgg
    27841 gaccattatc cctgaaaatc cacgtattac caatgcccaa aaggctatcc gtaccaacga
    27901 catcgaaaac gtagggaaga ctgcgcgtca ccataccatg tttgaaatgt tggggaactt
    27961 ctctatcggg gattacttcc gtgacgaagc tatcacttgg gcttatgagc ttttgacaag
    28021 ccctgaatgg tttgatttcc ctgctgaaaa actttacatg acctactatc cagacgataa
    28081 agattcttac aaccgctgga ttgaagtggg agtggatcca agtcacttga ttccaattga
    28141 ggacaacttc tgggaaatcg gtgcgggacc ttctggacca gatacagaaa tcttctttga
    28201 ccgtggggaa gcctttgacc cagaaaatat cggtcttcgc ctgcttgcag aagatattga
    28261 aaacgaccgt tatattgaaa tctggaacat cgttttgtca caatttaacg cagaccctgc
    28321 tgttcctcgt agcgaataca aggaattgcc acataagaac attgatacgg gcgctggttt
    28381 ggagcgtttg gtggctgtta tccaaggggc taagaccaac tttgaaacgg acctctttat
    28441 gccgattatc cgtgaagtcg agaaattgtc tggtaaggtt tatgaccaag atggcgacaa
    28501 catgagcttc aaggttatcg cagaccacat tcgttctctt tcatttgcta tcggtgatgg
    28561 tgcccttcca ggaaatgaag gtcgtggtta tgttcttcgt cgtcttctcc gtcgtgcttc
    28621 tatgcatggt caaaaattgg gtatcaacga gcctttcctt tacaaacttg ttccaactgt
    28681 tggaaaaatc atggaaagct actacccaga agtgcttgag aaacgtgact ttattgagaa
    28741 aatcgttaag agcgaagaag aatcatttgc ccgtaccctt cactcaggtc aacactttgc
    28801 ccaaggcatt gtggcagact tgaaagaaaa aggtcaatct gtcattgctg gtcaagatgt
    28861 ctttaaactc tatgatacat acggattccc agttgaattg actgaagaaa ttgctgaaga
    28921 agctggtatg actgtagact gtgaaggttt tgaagcagcc atgaaagaac agcaagaacg
    28981 cgcgcgtgcg tcagctgtca agggtggctc aatgggtatg caaaatgaaa ctcttcaaaa
    29041 catcactgta gaaagtgtct tcaactacaa tgctagccaa ttgtcttcta aattggtagc
    29101 tatcgttgct gacaatgcag aagtaggagc tgtttcagaa ggaactgcct ctcttatctt
    29161 tgcggaaacg tcattttatg ctgaaatggg tggacaggta gctgactacg gacaaatctt
    29221 ggatgagtca ggtaaggttg tggctactgt gaccaatgtt cagaaagccc caaatggtca
    29281 agcccttcat acagttgaag tccttgcacc gcttgccttg aaccaagaat ataccttggc
    29341 aattgatagc aatcgccgtc accgtgtcat gaaaaaccac actgcgactc atttgcttca
    29401 cgctgccctt cacaatatcc ttggaaacca cgcaacacag gcaggatctc ttaacgaagt
    29461 tgaattcctt cgctttgact ttacccactt ccaagcagta actgctgagg aacttcgtgc
    29521 gattgaacag caagttaacg agaaaatttg ggaagcactt gaagtgaaga cagttgaaac
    29581 ggatattgac actgctaaag aaatgggagc tatggccctc tttggtgaga aatacggcaa
    29641 ggaagttcgt gtcgtgacta tcggtgacta ctctattgaa ctttgtggtg gtactcatgt
    29701 tgacaacact tctgagattg gtctcttcaa aattgtcaaa gaagaaggaa tcggttcagg
    29761 aactcgccgt atcttggcag tgactggtaa ggaagccttt gaagcctatc gtgaacaaga
    29821 ggatgctctt aaagctatcg cagcaacctt gaaagcacct caagtcaagg aagtacctca
    29881 caaggtagaa ggacttcaag aacaacttcg tcaacttcaa aaagaaaatg ctgagttgaa
    29941 agaaaaagcc gcagctgcag ccgcaggcga tatcttcaaa gatgttaagg aagtcaacgg
    30001 tcatcgttac attgctagtc aagtgtctgt atccgatgcc ggtgcccttc gtacttttgc
    30061 agataactgg aaacaaaaag actactctga tcttcttgtc ctagttgccg ctatcggtga
    30121 caaagtcaat gtccttgtag caagcaagac aaaagacctt catgcaggaa accttgtcaa
    30181 agaattagca ccaatcatcg atggacgtgg tggtggtaaa ccagacatgg ccatggcagg
    30241 aggaagcaac caagctaaga tccaagaatt attggatgca gtagcaggta aattgtaatc
    30301 aagtaaagat ctatccattt gggtaggtct tttttgacta caaaaaagcc aaatccgctt
    30361 ggacctagct tggttattat gagctattag atggtattga ctgcccagac acttacggat
    30421 ctagctgaga cagggaattg tccataacct tcctcatcaa ttgtaacttg accttggtgg
    30481 ttaccaagta aatctacaaa ggtttgatta gtccattctt gaccgacaaa cattgacttg
    30541 ctgttttctt ggtcatttga aatcaagact gcgattgggg attgattttc agcacctgaa
    30601 cgtacccaac cgatacagtt agcatggtca aagtagtcat tttgttctcc ataggccaaa
    30661 tcttttcgga tggctaggag gcggtcaagg atttctttga aatcttcttg agcatactgg
    30721 cctgaaatcc catagtagtc tccgtaaaag acacatggaa ggccgtcttg gcgtaacaaa
    30781 atgagggcat aggctgctgg cttgaaccat tcttcaacgg tagactcaag ggcttgtcct
    30841 cgttgggtat cgtggttgtc gacaaaagtc acagccttgt caggcttgag ttcaaccagg
    30901 ctatctgtga aaatgccacg aaggtcatag tttgcgccag cttgactggc ttcaaagaga
    30961 ttctggtgga gacgaacatc gacaaggtca aagtgttctt ccgttttttc gagatagtcc
    31021 agattggctt ccttgtctgg gttccaaaat tcaccaaaaa catagaaatc gtcaccgtat
    31081 ttttccttca tatcgcggat gaaattgcgc ataaagaaag agtcaatatg cttaacggca
    31141 tccaaacgga aaccagctac accagtcgtt tccatgaacc aatcagccca gtcatagatg
    31201 ttttggatga cttcaggatg tttaaagtct aggtcggcat acatgaggta gtcgtagttt
    31261 ccgttttcgt tatcgaccaa tccctcgtta gcccagccct tgttgtctcc ttggattagg
    31321 taaatcccgg atttacggcg tttggcatca tagtctgtac ctgtgaagtg gtaccagtgc
    31381 cagtggaagt cattgtaggt atcttggcga ccatcgaagg taaaactagt ccagccattg
    31441 atggtgaagg gttctccaag ttcaactgta cggtctacag gatccacttc aataacctga
    31501 aaggcttccc tgtgatcagc agcagccttg tggttgagaa ctacatcggc cataggttga
    31561 attccctgcg ctttaagggc ttgaatggct tgaagatagt cttctttgaa accatacttg
    31621 gtgcggacag tccctttttg gttgaactct cctaagtcaa ataagtcata gaccccatag
    31681 ccgacatctt tttcgttggt tgccttgaag gctggtggca tccagacgtg actgatcccc
    31741 agatgagcta ggtgtggagc attttcagcc agacgcgtcc agtgttgacc gtcgtggggc
    31801 agataccatt caaagtattg catgagtgtt tgattttgca tgatgtttcc tcttacttgt
    31861 caatcttgtt ctttattcta tcataaagta tctctcggag caaacgtttg cgtaagatag
    31921 aaggaaacta ttttaactac ttaaaataaa gtattgattt ttttgtatca aagtgctagt
    31981 atagcgatgt ataatacaga taaagatagg agtcatccca tgattgaatt tcaaaatgtt
    32041 agtaagttgt atggtgataa agaggccttg agtaatctca atttgcagat tgaaaatgga
    32101 gagattatgg gcttgattgg tcataatggg gctggaaaat cgaccaccat aaaatcccta
    32161 gtcagtatca tttcacccag cagtggtcgt attttggtag atggtcagga gttatcggaa
    32221 aatcgcttgg cgatcaaacg aaagattggc tacgtagcag actcgcctga cttattttta
    32281 cgtttaacgg ccaatgaatt ttgggaattg atcgcctcat cctatgatct gactagctct
    32341 gacttggaga ctagtctagc taggctattg aacgtttttg attttgctga aaatcgttat
    32401 caggttattg aaaccttttc tcatggaatg cgtcagaaag tttttgtcat cggggcactc
    32461 ttgtctgatc ctgatatttg ggtcttggac gaacccttga ctggtttgga tccccaggct
    32521 gcctttgatt tgaaacagat gatgaaggaa catgcacaaa aagggaagac agtcttgttt
    32581 tcaactcatg tcctagaggt ggcagagcaa gtctgtgatc ggattgccat tttgaaaaag
    32641 gggcatttga tttattgtgg tagtgtagag gacttgagaa aagattaccc agaccagtct
    32701 ttggaaagta tctaccttag tcttgctggt agaaaagagg aggtttcaga tgcgtctcaa
    32761 ggtcattaaa aaattagttg atatcaatat cctctattca tctcaagaag ctaatctggc
    32821 taacctacga aagaagcaag ctaaaaatac tggaaaaaaa gtaaatgttt ccgctagagt
    32881 cctaagttct tacatttttt ccagtctctt gatgcttttc atgtttataa atatagcctt
    32941 tcgttttcct tttgaggaaa tgccaagttt ttttagtacg atggttgcta ttttactggt
    33001 gctggccttt tcaacttctt tcactgcatt ttacaatgtc ttttatgaga gtaaggacct
    33061 ggcctcctat aggccctatg cctttaaaga atcagagatt ataatggcca aaggtctgtc
    33121 tgtcctcttg ccagctctag ctggaattgt accaatccta gcttattttc tggtcctata
    33181 cattaggcta gcaccttctc tgtggttggg tttgcctttg atgctactgt ccttgacctt
    33241 attatttgtc tctgttactt tagtgatggt agtggcagta catttcttgg ctcagactag
    33301 ggtcttcaga aagtatcagt ctattttttc gaatgtgatg attgggatag gagttctcat
    33361 acctttaata tttatcttct ttcttcagtc gacttttgga agtattgttg acaaagttag
    33421 agacattcca tttctccttt atcctcttca tatcttttac aaaatagcag tggagccttt
    33481 ttcgacagaa gccttagtgg gtctgctcgc ttggatagga ctaactctct tcctgcttta
    33541 tctgaccaaa aagaaggtcc ttcctcgttt ttatgacgtg atcctgctta atagtgagga
    33601 taaggtcaaa aaagaacgtc gcagcaagga gagaatttca accactaaaa agggattttt
    33661 tcgcatggtt ttacgctacc acctcaccct cttgggacag ggtactggcg tggttacagt
    33721 gctttttaca agtgctttct ttccttatct catgatgatc ggtctgattt ccaaaatccg
    33781 agattctcag atagttccag acattcatcc tccatactgg ttacccttgt tttttatagc
    33841 tctctttata gcagttgtca ataacaatat caccagcctg cattcaattg ccttgtcctt
    33901 ggagagggaa aatgttgatt ttcttaagag tttacccttt gactttgctc gttatgtgaa
    33961 agtgaaattt tggattatat atgctgtcca gtccttttta ccagttctga ctttgcttgg
    34021 tctttctcta tatctaggct tgcccatcat ttcgatgatt taccttattg tggcatggat
    34081 ccttgccagt gtcatccttt cttgccacca ttactttaag gacgttaaaa atctgtcaac
    34141 caattggagt agcattacgg acctggtgaa tcgttcaaat ggcatagtcg ccatagtttt
    34201 attgtttatt tatagtgcaa tcctgatggc ccttgtaatt gggagcatat tcttggttca
    34261 gtctctctcc actatccttg ccatcagctt gggagtagga gctcttatcc tcctgcttgc
    34321 tcttgctatt tttggctatc attattacct gtcacgcata ttggcagaaa tagaaaaaag
    34381 atgagctggt tggtcgcttg cttgataaat tcaattcgac aaacgctttt atagtttgtt
    34441 agaatgtagt tgtaaatgat taagattggt aatcaaaaat atagggagga atgttttgaa
    34501 taagaaatat aacatagttc tatttttgtt gtttatagtt tatttatttg gatatttttc
    34561 aatttcaaaa acgttaattc ctataatgtg tgtgttccaa gtattcttga tagaacatat
    34621 atttagaatt cgaaatagaa tgatgcagat aggtgaaatt ataattattg ttgcttctat
    34681 aatattattt attgatagta tattgagttt gtaacaaaag gcttctctat aaaatcaaat
    34741 tttccagata tctactgaag ctgatttatt tattcatctt tcttctacta cctttgtcac
    34801 taaatttgac agacacatat tgctgataca acctctctac ctataatatc acagttaacc
    34861 tgaggctagt ttcatagttt gctatttgat tttcgttgat aaaaaaaatg ctcctccaag
    34921 acggaagagc attttttatt gtgattctta cacatttact tcctagtttt gcaggaagtc
    34981 tagtatacca ttttttaaaa gaagagtaaa aaagcaaggg tagtttcttt tttgagaatt
    35041 gtctaaaagt ttgatataat ggtagttatg aatagaataa gagtcagcaa aagggttgaa
    35101 aagaagcttg ctaaggggct agttttacta gaagccagtg atcttgagaa tgtcaatctt
    35161 aaggatcagg aagtagaggt gcagggtcag gaaggaaact ttcttgggac tgcctacctt
    35221 tctcagcaaa acaagggctt gggctggttt gtcagcaaag acaaggtggc cttcaatcaa
    35281 gctttctttg aaacgttgtt tagaaaagcc aaagaaaaga gaagcgccta ctatcaagat
    35341 gatttaacaa ctacctatcg tctctttaat caagagggag atggctttgg gggtctgaca
    35401 gtggaccttt atggcaacta cgccgtcttt tcttggtata actcttatgt ttatcagatt
    35461 cgtcagacta tatcagaagc ctttagacag gttttccctg aggttttagg ggcttatgag
    35521 aaaatccgct ttaaaggttt ggactatgaa tctgcccatg tttatggtca agaagcacct
    35581 gactttttca ctgttttaga aaatggtgtc ctgtatcaag tctttatgaa tgatggtttg
    35641 atgacaggaa ttttcctaga ccagcatgag gttcgcggta gtttagttga cggcttggct
    35701 atgggtaaat ccttgctcaa tatgttttcc tacacagcgg ctttttcagt agctgcggcc
    35761 atgggaggag ctagccagac aacttctgtt gatctagcca aacgttcacg agaattgtct
    35821 caagcgcatt ttcaggcaaa tgggctcagc acagacgagc atcgttttat agtcatggat
    35881 gtctttgagt atttcaaata tgccaaacgc aaagacttga cctacgatgt gattgtccta
    35941 gatccgccta gctttgctcg gaataaaaaa caaactttct ctgtggccaa ggattatcac
    36001 aagttgattt cccagagtct tgagatttta aatccgggag ggattatcat tgccagtacc
    36061 aatgctgcca atgtttcccg ccagaaattt acagaacaaa ttgataaagg ctttgcagga
    36121 agaagttacc agattttaaa caaatatggt cttccagcag attttgccta taataaaaaa
    36181 gatgaaagta gtaattacct caaggtgatt agtatgaagg ttagtaaatg aaattaatcg
    36241 tttcagtaat gccaagaagt ttagaggagg ctcaggctct ggatgccacg aggtacctgg
    36301 atgccgacat cattgaatgg cgtgccgact atctgcctaa agaagcgatt ttgcaggtag
    36361 ctccagccat ttttgaaaaa ttcgcaggcc gtgagttggt tttcacgcta cgaactcgct
    36421 ccgaaggtgg agaaatcgac ctttctccag aagagtatat ccatctaatc aaggaagttg
    36481 cgcaactcta tcaaccagac tatattgatt ttgagtacta tagctacaag gatgtttttg
    36541 aggaaatgct ggacttccca aatcttgttt tgagttacca taatttccaa gaaacacctg
    36601 agaacatgat ggaaatcttg tcagagttga cgatcctaaa tccaaaactt gttaaggttg
    36661 cggtgatggc tcacacggag caggatgtct tagacttgat gaactataca cgaggcttta
    36721 aaaccctcaa tcctgaacag gaatatgtga ccatttctat gggtaaggtg ggcaaggtct
    36781 ctcgtatcac tgcggatgtg actggttcga gttggtcttt tgccagtctg gatgaggtta
    36841 gtgcccctgg gcagatttct ctagctagca tgaaaaaaat cagggagatt ttggatgaag
    36901 cttgatggct atacacgttt agctgcagtt gttgccaatc ctattaagca ttctatttct
    36961 cccttcatcc acaatagagc ctttgaggcg acagctacca acggtgctta tgtggcttgg
    37021 gagattgaag cgagtgactt ggcagaaaca gtggccaata ttcgtcgcta ccagatgttt
    37081 ggcatcaatc tgtccatgcc ctataaggag caggtgattc cttatttgga tgagctaagc
    37141 gatgaagcgc gcttgattgg tgcggttaat acggttgtca atgagaatgg caatttaatt
    37201 ggatataata cagatggcaa gggatttttt aagtgcttgc cttcttttac aatttcaggt
    37261 aaaaagatga ccctgctggg tgcaggtggt gcggctaaat caatcttggc acaggctatt
    37321 ttggatggcg tcagtcagat ttcggtcttt gttcgttccg tttctatgga aaaaacaaga
    37381 ccttacctag acaagttaca ggagcagaca ggctttaaag tggatttgtg tgctttagaa
    37441 tatgtttctg aactgcaagc aaggattgcc gagtcggatt tgctagttaa tgccaccagt
    37501 gtgggcatgg atggccaatc ctctcctgtt cctgaaaaca tagtcttacc agaaactctc
    37561 ttagttgcag atatcatata ccaacccttt gaaacaccgt ttttgaaatg ggctagaagt
    37621 cagggcaatc cagcagtcaa tggtctggga atgttgctct atcaagctgc agaagctttt
    37681 caactgtgga caggcaagga aatgccgaca gaagagattt ggcagtcttt aacagaaaaa
    37741 taccaataaa gaaagggaga ctctcctatg aaaatcagaa tcgatattcc tcatcatcct
    37801 tatgatattc agattgaaaa aggttgtatg gcccaggctg gtcagtggtt gcgagaactc
    37861 tggcaaccgc aaaaggtagt cattgtgaca gataaccatg tagcctctct ctatgcagag
    37921 aaggtcaagc tcagcctaga agatgctggt tttcaggtag ctgtttttga tttcttagaa
    37981 ggtgaagaaa gaaagaattt aactactgtt cagaaagtct atgaatttct agtcaagcaa
    38041 ggtctgactc gtagcgatgg aatcgttgct cttggtggtg gcgttgttgg ggacctggct
    38101 ggctttgtag cctctaccta tatgcggggt attcactttg ttcagattcc gactagtttg
    38161 acagctcagg ttgattcttc tatcggtgga aagacaggcg ttaacacacc atttgctaaa
    38221 aatatggtgg ggacctttgc ccaaccagat ggggttctga ttgatccact tgttcttgaa
    38281 accctcggaa aaagagagtt gattgaaggg atgggtgaag ttatcaagta tggcttgatt
    38341 gaggatccag aactgtgggc tctcttgacg ggactgaatg gttctgttga gagtattttg
    38401 gaacatgcag agaccttgat tgaacattct tgtcaggtga agcgcaagat ggtggttgaa
    38461 gatgagttgg acaatggtat tcgtctttac ctcaattttg gccacactat tggccatgcc
    38521 atcgaagcga ctgccggtta tggcaaggtc atgcatggag aggctgttgc catgggaatg
    38581 gtacagattt ccaaggttgc tgaggaaaaa ggcctcatgc cagctggcat tacccaatct
    38641 atcacagaga tgtgtcagaa attcggcttg cctgttgact atgaaaattg gaaagttgac
    38701 aagctttatc aggctcttac tcatgacaag aaagcgcgtg gtaacacctt gaaattggtc
    38761 ttggtgccag agcttggttc agcgaccatt cacccagttt ctctggaaga gatgaaagac
    38821 tacttggtaa aataaggaga gtgtatgaga tatttaactg caggagaatc acacggcccc
    38881 cgtctaacag ctattattga gggaattcca gctggacttc cattgacagc tgaggatatc
    38941 aatgaggatc ttagacgccg tcagggtggc tacggtcgtg gtggtcgtat gaagattgag
    39001 agtgaccagg ttgtctttac ttcgggtgtt cgccacggga agacgacagg ggcgcctatt
    39061 actatggatg tcatcaataa ggaccaccag aaatggctgg acatcatgtc tgcggaggac
    39121 attgaagacc gccttaaaag caagcggaaa attactcatc ctcgcccagg tcatgccgat
    39181 ttggttgggg ggattaagta ccgttttgat gatttgcgaa attctttgga gcgttcatca
    39241 gctcgtgaaa ccaccatgcg ggtggcagtt ggtgcagtag ccaaacgcct cttggctgag
    39301 ctcgatatgg agattgccaa ccatgtcgtg gtctttggtg gtaaggaaat agatgttcct
    39361 gaaaatctga cagtcgctga aattaagcaa agagctgccc agtctgaagt ttctattgtc
    39421 aaccaagaac gagaacagga aatcaaggac tatattgacc aaatcaaacg tgatggtgat
    39481 accatcggtg gggttgtgga gacagtcgtc ggaggcgttc cagttggtct tggttcctat
    39541 gtccaatggg atagaaaatt ggatgcaaga ttggctcaag ctgttgtctc tatcaatgcc
    39601 tttaaagggg tggaatttgg tcttggcttt gaggctggtt atcgtaaagg cagccaagtt
    39661 atggatgaaa ttctctggtc taaagaagac ggttatactc gccgtaccaa taatctaggt
    39721 ggttttgaag gtggtatgac taatgggcaa cccatcgttg ttcgtggggt catgaaaccc
    39781 attcctactc tttataaacc tcttatgagt gtggatatcg aaacccacga accttacaag
    39841 gcaaccgtgg agagaagtga tccgactgct cttccagctg caggaatggt catggaagca
    39901 gttgtagcaa cggttctggc gcaagaaatc ctcgaaaaat tctcatcaga taatcttgag
    39961 gaactaaaag aagcggtagc caaacaccga gactatacaa agaactatta aggagttcct
    40021 atggcaaaaa caatctatat cgcaggtctt gggttgattg gagcctctat ggcacttggt
    40081 atcaaacgcg atcatccaga ttatgaaatt ttaggttata atcgtagtca agcttcgaga
    40141 gatatcgcct tgaaagaagg tatgattgac cgtgcaacgg atgattttgc tagttttgct
    40201 cctttggcag atgtcattat cctcagcttg ccaatcaaac aaactattgc tttcattaag
    40261 gagttggcca atttggattt gcgagaaggc gttattattt cagatgctgg ttcgaccaag
    40321 tcaaccattg tggatgcggc ggagcagtat ttggctggca agtctgttcg ctttgtcggg
    40381 gcccatccca tggctggtag tcacaagaca ggggctgctt cggcagatgt caatcttttt
    40441 gaaaatgcct attatatctt tacaccttca agcctgacaa gtcaggacac gcttaaggaa
    40501 atgaaggatc tgctttcagg tcttcatgct cgttttatcg agattgatgc caaggagcat
    40561 gatcgtgtca cttctcagat tagccatttt cctcatattt tggcttctag tctcatggag
    40621 cagactgcgg tctatgctca agagcatgag atggcaaggc gctttgcggc aggtggtttt
    40681 cgagatatga cccgaattgc ggaaagcgag ccaggaatgt ggacctccat tctcttgtcc
    40741 aatagcgaga tcattctgga tagaattgag gatttcaagg aacgtttgga agcgattggt
    40801 caggccatta gtaagggaga tgaagagcaa atttggaact tttttaacca agcgcgtgaa
    40861 caacgtcagg ccatggaaat ccataagcgt ggaggtgtgg atagttctta cgacctctat
    40921 gtcgacgttc ccgatgaaga agatgtcatc ttgaggattt tggaactact acgtggaact
    40981 tccttggtca atattcatat caacgaggaa aaccgtgagg atattcacgg gattctacaa
    41041 atttcattta aaaatgctca agacttggaa agagctgagc atctcataac agaaaatacc
    41101 gactacacag tcgttgtcaa ataaggagaa aatcatgtca aatatttacg atagtgcaaa
    41161 cgaacttagt cgcggtctac gcgaattacc agaatacaag gctgtcaaag cagctaaaga
    41221 tgcgattgca gcagatgctg aggcaagcaa aatctttaca gattatcttg cctttcaaga
    41281 ggaaattcaa aaactagcac agacaggtca aatgccagat gcgtccttcc aagctaagat
    41341 ggaaggcttt ggcaagcaga ttcaaggaaa tagcctcttg tcagaattct ttactaagca
    41401 acaacaattg gccatttacc tttctgacat tgaaaaaatt gttttcgaac cagtttcaga
    41461 attgctaaaa taagaaaata actatcctaa agtcaggaat ttttaaggaa tttctggctt
    41521 tttatgataa aataggtaaa agtattgcaa gtatgaggtc cagtatgaaa ctaaaaacaa
    41581 acattagcca cttacatggc agtatccgcg tcccaggtga caagtctatc agccaccgtt
    41641 ccattatctt tggaagttta gctgagggag agaccaaggt ttatgatatt ttgcggggtg
    41701 aagacgtcct ttcaaccatg caggtctttc gtgatcttgg tgtagagatt gaggataaag
    41761 atggggttgt taccattcaa ggtgtcggta tggacggcct aaaagctcca caaaatgccc
    41821 ttgatatggg aaattctgga acctcgattc gcctgatttc aggtgtcctt gctggtgcag
    41881 acttcgaagt agaaatgttc ggagacgata gtctttccaa acgtcctatg gaccgtgtga
    41941 cgattccact gaaaaaaatg ggcgttagca tttcagggca aactgagcga gacttgcctc
    42001 cccttcactt aaaagggacg aaaaaactag cacctattca gtatgagttg ccaattgcct
    42061 ctgcccaagt caagtcagcc ttgatgtttg cggccttgca ggctcaggga gagtcagtga
    42121 ttatcgaaaa agaatgcacc cgtaatcata ctgaagatat gctgcaacaa tttggtgctc
    42181 atttaagtgt ggatggcaag aaaatcacag tccgaggact tcaaaaactg acaggacaaa
    42241 aggtggttgt tccaggagat atttccagtg cagccttttg gttggtcgca ggtttgattg
    42301 ttccaaattc tcgtctagtg ctgcagaatg tggggatcaa cgaaactcgc accggtatta
    42361 ttgatgtcat tcgtgccatg ggtggaaaat tggaaataac tgaaatcgat ccagtcgcta
    42421 aatctgctac cttgattgtt gagtcttctg acttgaaagg aacagagatt ggtggcgctt
    42481 tgattccacg tttgattgat gaattgccca ttattgccct gcttgcgact caagcccaag
    42541 gtgtaacagt tatcaaggat gctgaggagc tcaaggtcaa ggaaacagac cgtattcagg
    42601 ttgtggcaga cgccttaaat agtatgggag cagatattac tcctacggca gatgggatga
    42661 ttatcaaagg gaaatcagct cttcacggtg ctagagtcaa tacgtttggt gaccaccgta
    42721 tcggcatgat gacagctatc gcagccctct tggttgcaga tggagaggtg gagcttgacc
    42781 gtgcagaagc catcaatacc agctatccta gtttctttga tgatttggag agcttgattc
    42841 atggctaagg tgttattagg gtttatgggg gctggaaaat cgactattgc aagaggcttg
    42901 gaccctaatt accttgatat ggatgctctg attgagaagc gcctaggtat gtccattgcg
    42961 aattttttcg ctgaaaaggg agaagcagcc tttcgtcatg tagaatcaga agtcctagct
    43021 gatttactac aaacagacca agtcgtgtca actggaggag gagtggttat ttctcagaga
    43081 aatcgtgact tactcaagac taatacagat aacatctacc tgaaagcaga ttttgaaacc
    43141 ctctacctac gtatcgcagc tgataaggac aatcagcgac cgctttttct aaataatagc
    43201 aaggaagaac tagtagctat ttttcaagaa agacaggctt ggtatgagga agtggctagt
    43261 cgggttttgg atgtgaccaa gctaagccca gaggaaatta tagaggaact aagatgaaaa
    43321 tagcttatct aggtcccaag ggatcatttt cacaccacgt tgtgcagaca gcttttcctc
    43381 atgaggaatt gcaggctttt gccaacatta cagatgtcat caaagcttat gagcaaggat
    43441 tagtgaacta ttctgtggtg ccagttgaaa attctattga gggtagtgtt catgaaacct
    43501 tggactatct ttttcatcag gctcacatcc aagcagtagc agaaatcgtt cagcctattc
    43561 atcagcagtt gatggtggtt ccaggccata ctaagattga aaagattttt tcacatccac
    43621 aggccttggc tcaaggaaag aaattcatcg atgaacaata tccagaggct caaatcgagg
    43681 taacagctag tacagcttat gcggcccgtt ttatttccga acatccagac cagccctttg
    43741 cagcagttgc acctagaagt tctgctgaag aatatggatt ggaactgatt gccgaggata
    43801 ttcaggaaat ggaagccaat ttcacacgtt tctgggttct aggagctgaa aagcctagta
    43861 ttcccttgca agcacaaact gaaaagatga gtttggcctt gacattacct gacaaccttc
    43921 caggtgcact ttataaggcc ctgtcgacct ttgcttggcg aggaattgac ttgacaaaaa
    43981 ttgaaagtcg tccactcaag acagcactgg gtgaatactt tttcattatc gatgtggatt
    44041 ataccgataa ggacttggtc cactttgccc aaaaagaatt agaagcgatt ggaatccagt
    44101 ataaaattct gggtgcctat cctatttatc caatatcaga ccatggaaag gagagaagat
    44161 gagtaaagaa aatcccttaa gtcatcatga gcagttgcgt tatgattatt tgctaaaaaa
    44221 tattcactac ctcaatgaga gagaaaaaaa tgagtttgcc tatttgcaag aaaagctaac
    44281 tcttgctagg ggaaatagta gctctagctt ggaacaagaa agagaagagc aggttgactt
    44341 accaagctat gcgaaccgga gtcgctcaca atccaaatca caagcactct ctttccctcc
    44401 aaaaaagaaa agacggaagc tccgtcttaa acgaattttt atggtgattt tttcactttt
    44461 ggtctgtgtg gctttggcca tggtattcat gtttttgcgt ggttaccaag atgctagcgc
    44521 aaagaaaact gctgatgccc gggcagctca agtagaagtc tttaatggtc aggacactag
    44581 agatggagtt aatattttaa tcatgggtac tgatggtcga atcggccaga acagtgttga
    44641 gacacgaact gactctatta tggtattaaa tgtcgggggc tcagataaga aaatgaagct
    44701 agtcagtttc atgcgtgata atttggtcta tatagatggt tatagtcaag tgattaacgg
    44761 tagaaaacag acagataaca agttaaacgt agcctacgag ttaggagaac aagaggggca
    44821 aaaaggggca gaaatggttc gccaagtctt gaaagataat tttgacttgg acattaagta
    44881 ctacgccttg gttgatttcc aggcctttgc cacagcgatt gacacgcttt tccctgatgg
    44941 ggtgacgatt gatgctcaat tttcaacatt gaatgggcgt cccctaacag aagctacagt
    45001 cggagatgat ttatatgcta ccgagactga gtctccaacc caaaccatta aagtcggaaa
    45061 acagcagatg aatggctcga ccctgctcaa ttatgctcgt ttccgtgatg atgatgaggc
    45121 ggattacggc cgtacaaaaa gacagcaaca agttttaaca gcaattttag agcaaattaa
    45181 agatcccact aaacttttca ctggttctga agctcttgga aaggtttttg ctatgacctc
    45241 aaccaacgta ccctatactt tcctcctaac aaacggttta tctgttttgg atggagcaaa
    45301 aaatggtatt gaaaaattga cgattccaga actgggtgac tgggtagatg cctatgatgt
    45361 ttatggaggc ttgggcttgc tggttgatca aaacaaatat caaaccaagc tcgctcaaat
    45421 gggcttgaga taagataata ctcaatgaaa atcaaagtgc aaactaggaa gctagccgca
    45481 ggctgctaaa aacacagttt tgaggttgta gataagactg acgaagtcag ttacctatac
    45541 ctacggcaag gtgaagctga cgtggtttga agagattttc gaagagtata agacagaaaa
    45601 atcaaagttt gatagctatt tggatagcag tcaggctttg attttttaca gtctgcaata
    45661 gattttcacc ccttgtttgt ggtataatga aacgatacga tagaaagaat aatgaacaat
    45721 atgactgatt taaaagcaat tcaggctcgt agtctggaga tggctgaata ttttgtggcc
    45781 ttttgtaaag aacatgattt gctctgttat ctctgtggcg gaggcgctat tggtgccctt
    45841 cgaaacaagg gatttattcc ttgggacgac gacctagact tttttatgcc tcgtaaagat
    45901 tatgagaaat tagcagaatt atggcctcgt tatgcagatg aacgttattt cttgtcaaag
    45961 agtcacaagg attttgttga tcgtaatctt tttattacca ttcgtgacaa ggaaaccacc
    46021 tgtatcaagc cttatcagca ggatttggat ttgccacatg gtctggcctt ggatattttg
    46081 cctttggatt attatccgaa aaatccagct gagcggaaaa aacaggttcg ttgggccttg
    46141 atttattcac tcttttgtgc gcaaactatt ccagaaaagc atggtgctct catgaaatgg
    46201 ggaagtcgca ttttactggg tttgactcca aaatctctcc gttatcgcat ctggaaaaaa
    46261 gctgagaaag aaatgactaa gtatgatttg gctgattgtg atggcattac agaattatgc
    46321 tcaggtcctg gctacatgag aaacaagtac ccaatcacat cttttgaaga caatcttttc
    46381 ttgccatttg aaggaacaga gatgcctatt ccaatcggct atgatgtcta tctcagaact
    46441 gcttttgggg attatatgac gcctccacca gcagacaagc aggtaccgca tcatgatgct
    46501 gtcatcgctg atatggataa gtcttataca gaatacaagg gagaatatgg tggctaagaa
    46561 aaaaatctta ttttttatgt ggtctttttc tcttggaggt ggtgcagaga agattctatc
    46621 aaccattgtt tcaaatctgg atccagaaaa gtatgatatt gatattcttg aaatggagca
    46681 ctttgacaag ggatatgaat ctgttccaaa gcatgtacgc attttaaaat cccttcaaga
    46741 ttatcgccaa accagatggt tacgagcttt tttgtggaga atgagaattt attttccaag
    46801 actgactcgt cgtttgcttg taaaagatga ttatgatgtt gaagtttctt ttaccattat
    46861 gaatccacca ctgttgttct ctaaaagaag agaagtcaag aagatatctt ggattcatgg
    46921 aagtattgaa gaacttctta aggatagctc taaaagagaa tcacatagaa gccagttgga
    46981 tgctgcgaat acaattgtag ggatttcaaa aaagaccagc aattctatca aggaagttta
    47041 tccagattat acttctaaat tacagacaat ctacaatgga tatgattttc agactattct
    47101 agaaaaatct caagagaaga tcgatatcga gattgctcct caaagtatct gtactatcgg
    47161 acggattgag gaaaataagg gttctgaccg tgtagtggaa gtgatacgat tattacacca
    47221 agagggaaaa aactatcatc tctattttat cggggctggt gatatggaag aggaactgaa
    47281 aaaacgagtc aaagagtatg ggattgagga ctatgtacat ttccttggtt atcaaaaaaa
    47341 tccttatcag tatctatctc agacgaaagt tcttttgtct atgtctaaac aagaaggttt
    47401 tcctggagtg tatgtggagg ccttgagtct gggactccct tttatctcta cggacgttgg
    47461 aggggctgag gaattatccc aagaaggacg atttggacaa atcattgaga gcaatcaaga
    47521 ggcagctcag gcgattacta attacatgac ttctgcctca aactttgatg tcgatgaggc
    47581 tagccaattc attcaacaat ttacaattac aaaacaaatc gaacaagtag aaaaactatt
    47641 agaggagtag catggaaact gcattaatta gtgtgattgt gccagtctat aatgtggcgc
    47701 agtacctaga aaaatcgata gcttccattc agaagcagac ctatcaaaat ctggaaatta
    47761 ttcttgttga tgatggtgca acagatgaaa gtggtcgctt gtgtgattca atcgctgaac
    47821 aagatgacag ggtgtcagtg cttcataaaa agaacgaagg attgtcgcaa gcacgaaatg
    47881 atgggatgaa gcaggctcac ggggattatc tgatttttat tgactcagat gattatatcc
    47941 atccagaaat gattcagagc ttatatgagc aattagttca agaagatgcg gatgtttcga
    48001 gctgtggtgt catgaatgtc tatgctaatg atgaaagccc acagtcagcc aatcaggatg
    48061 actattttgt ctgtgattct caaacatttc taaaggaata cctcataggt gaaaaaatac
    48121 ctgggacgat ttgcaataag ctaatcaaga gacagattgc aactgcccta tcctttccta
    48181 aggggttgat ttacgaagat gcctattacc attttgattt aatcaagttg gccaagaagt
    48241 atgtggttaa tactaaaccc tattattact atttccatag aggggatagt attacgacca
    48301 aaccctatgc agagaaggat ttagcctata ttgatatcta ccaaaagttt tataatgaag
    48361 ttgtgaaaaa ctatcctgac ttgaaagagg tcgctttttt cagattggcc tatgcccact
    48421 tctttattct ggataagatg ttgctagatg atcagtataa acagtttgaa gcctattctc
    48481 agattcatcg ttttttaaaa ggccatgcct ttgctatttc taggaatcca attttccgta
    48541 aggggagaag aattagtgct ttggccctat tcataaatat ttccttatat cgattcttat
    48601 tactgaaaaa tattgaaaaa tctaaaaaat tacattagaa cgggggtgac caagtgattg
    48661 atgggaaacg attattattt agtttgacca tagtcagtta tgccttgacg ctagtaagtg
    48721 gaattgtgta tctgtttaat aataataatg ttagcttact ttctacttta ttgttcttgt
    48781 tggttagtag cttaattgct tgttggaatg atatcaagta ttacttaatc cattttattt
    48841 tctatttaac catttttgta tttctggtat caagaccgac cattgattat tttagggatg
    48901 gtgctttgga tacctatcat ccaatagcct atcgttttgc ctttatagtt gtcatgattt
    48961 cgattctggg cttgaccaca ggaggcattc tggctcgtta cttcatagct aggaagaaaa
    49021 taaaagtagc aaatatagga aattctctaa aagaggttta tatcaagcgg ttacgctttg
    49081 tatcactagg agtttttctt ctaacttatc ctttctattt cattcggtta tttgaacggc
    49141 tcttgtatcg tttgcagact tcctactatg cctactatgc aaattttgaa agtaaactgc
    49201 cttattttac ctacattttg tctaccttta cggtctatgc aatgtgtatg tatctggcaa
    49261 ccaagccaaa gaaattgcag gccacagcag tgcttgtctc ctttattgca gctaatacta
    49321 ttcatttggc aattgggaca cgaaatccct ttattttaag tattttattt gcttttgttt
    49381 attactttat gcgggagcaa actgaaaaag gaaaatggat tgggtttaaa gaaaagttag
    49441 cgatttttgt aggttctcct attctcatgt tagcgatggg agtactcaat tatgtacggg
    49501 ataatgtcca agtttcccat acaggtttct gggatatctt acttgacttt atctataaac
    49561 aagggactag ttttggtgtt ctggctcgag gttttctatt taacagtagc ctcccttacc
    49621 gagatttccg taattttact tttggtcctg ttcttgatta ttttgcaagg gggagtttgg
    49681 gagccatttt cggaggaaaa gcctttgaac atacaaccaa tagtgtggaa ctagctattg
    49741 atagtaatag ttatgcccac aatctatcct atcttgtctt gaacaaggaa tacttgaaag
    49801 ggcatggtat cggaagtagt tatatcatgg agttgtatac cgactatggt atgattggag
    49861 tctttctgct tagtttctta ctcggcgtat tatttatagc catgctgcaa gtagcctatc
    49921 gctcaaggac aatcctattt gctttatccc tactcatctt gaataatcta ttctttatgc
    49981 caagaagcag cttttcagaa agtttcttca atttatttac aatgcaattc tggggaattg
    50041 ttcttgtgat tatatttgta gcaaaaatgc ttacaaagga aaactagtat ctactaaaca
    50101 aaggagaaaa aaatcatgtt tgaacattat tcagtagctg atttgtttgc aaatctttat
    50161 aaaaagcgaa aagcaaatat tttagctctc atcgctttat ttgctctcat tgctgtacca
    50221 tttacaatta aagcagttag gaataaaaac actgtcaaag atacaacaag ttattcaact
    50281 tatcttatct ataaaatcac tcctccaaaa gagtcggcca aaacgatttt gaatcatcaa
    50341 attggtggtt atagtgattt ttatgggaaa ttgattgatg gtaatttgaa tggagcttat
    50401 cttttcaatg atgtagaacc cagtgagttg aaaaaaattg ccagtgaatt agacacgaca
    50461 gaaacaacct tgaaaaattc aacgaatgac tattggtgga aaaaattgac cgtctactat
    50521 atgattgacg atgcaggggt tggtgtgaaa attttgacat caagtaaaga tgccaatgac
    50581 ttgttagaga aaaaaattga tgggttgatt gagaaattta aacatgccta tgcaaatgtg
    50641 aaaattgaaa aactggaaac catcaactct aaagaattga acgcaaatgg tgaaacagcg
    50701 cttggcttaa atgtgaaaaa tctgattctt cgtttagttg ttattggagt ggtttgtgtg
    50761 attttggttg tgatgggaaa tgtgttagtt tatctcttta atccaacaat caatagagta
    50821 ggtgattttt ctcagtatca aattgatttt gtaacagaga ttacaacaat tgctaaccta
    50881 gcagatgttt tgtcatacaa aaatactgga caggaattga ccatcgttag ctcaaataaa
    50941 gctatcttag ataaattgaa acagagtcaa gaagctttaa aaggaatgca ttttgtagat
    51001 ttacaggatg tatcatctct tttggaaaga gatacagtcc ttcttgttga agagtacgga
    51061 gtgactcgtt ataagaaatt tgagcaaagt cttcaaattc tcagaaactt aaatcgttct
    51121 atcctcggtg tagcaacctt taaattataa gtattctgat gtataaggtc ttcaaaatcg
    51181 gctcttagcc gattttgaag gctttttcta tctgtctgca gaaattttct tttggagtct
    51241 cctatagaaa gaccctaggg aaaatctatc cgtatagagg agccttcttt tgataaaatc
    51301 gtaaaaatct agtataatag atagaactga aagtatgagg ttactagcaa tgaaaatgaa
    51361 acaaattagt gatacaactt taaaaatcac gatgtcttta gaggatttga tggatcgtgg
    51421 aatggagatt gctgactttc tcgttcctca agaaaaaaca gaagagttct tttatgctat
    51481 cttggatgag ctagagatgc ctgatagctt tctggataca ggcatgttga gcttccgtgt
    51541 gactccaaaa cctgataagg tagatgtctt tgtgaccaag tcaaagattg accaaaatct
    51601 agattttgaa gacttatcgg atttgccaga tatggaagaa ttggctcaaa tgtctccaga
    51661 tgaatttatc aaaaccctgg aaaaaagcat cgcagacaaa accaaggatg atatcgaagc
    51721 cattcaatct cttgagcaag ttgaagccaa ggaagaagag caagagcaag ctgaacaaga
    51781 agctgagagt aagaaagaac cttacatcta ctacatcctt tcttttgcta agttggctga
    51841 cttggtagct tttgccaaga cagtgacttt tgaaatggaa acttctgaac tctacaaaat
    51901 gaacgagcgc tattatttga ccattttagt ggatattgaa aatcatccaa gcccatatcc
    51961 agcttggctg ttggcccgta tgcgcgagtt tgcagacgat agtgatatca gtcgctcagt
    52021 cttacaagag tatggtcaag tcttgatgag tcacgatgca gtgctcaatc tgcaaaaaat
    52081 cggctaatcc tttctggaaa gctattttca gattttaaga agagcgaatc gtctgattcg
    52141 ttttttcttt ttatactgaa atagtgattt actataatag gaattttcac aaaattctgt
    52201 tataatggct atattagaaa atttcgagga gataaagatg acagttaaaa ttgctttact
    52261 aggatttggt accgttgcaa gtggtgtgcc tttcctccta aaggaaaatg gaggaaaaat
    52321 caatcaatca gcacattcag atatcaaagt tgctaaggta ttggtcaagg atgaagatga
    52381 aaaaaatcgc ttgcttgcag cagggaatga ctttaacttt gtaaccaatg tggatgatat
    52441 tttatcagac caggatatta ctatcgtagt ggaattgatg gggcgtattg agcctgctaa
    52501 aacctttatc actcgtgcct tggaagctgg aaaacacgtt gttactgcta acaaggacct
    52561 tttagctgtc catggcgcag aattgctaga aatcgctcaa gctaacaagg tagcacttta
    52621 ctacgaagca gcagttgctg gtgggattcc aattcttcgt actttagcaa attccttggc
    52681 ttctgataaa attacgcgcg tgcttggagt agtcaacgga acttccaact tcatggtgac
    52741 caagatggtg gaagaaggct ggtcttacga tgatgctctt gcggaagcac aacgtctagg
    52801 atttgcagaa agcgatccga cgaatgacgt agatgggatt gatgcagcct acaagatggt
    52861 tattttgagc caatttgcct ttggcatgaa gattgccttt gatgatgtag cccacaaggg
    52921 aatccgcaat atcacaccag aagacgtagc tgtagctcaa gagcttggtt acgtagtgaa
    52981 attggttggt tctattgagg aaacttcttc aggtattgct gcagaagtga ctccaacctt
    53041 cctacctaaa gcgcacccac ttgctagtgt gaatggcgta atgaacgctg tctttgtaga
    53101 atctatcggt attggtgagt ctatgtacta cggaccaggt gctggtcaaa aaccaactgc
    53161 aacaagtgtt gtagctgata ttgtccgtat cgttcgtcgt ttgaatgatg gtactattgg
    53221 caaagacttc aacgaatata gccgtgactt ggtcttggca aatcctgaag atgtcaaagc
    53281 aaactactat ttctcaatct tggctctaga ctcaaaaggt caggtcttga agttggctga
    53341 aatcttcaat gctcaagata tttcctttaa gcaaatcctt caagatggca aagagggtga
    53401 caaggcgcgt gtcgttatca tcacacacaa gattaataaa gcccagcttg aaaatgtctc
    53461 agctgaattg aagaaggttt cagaattcga ccttttgaat accttcaagg tgctaggaga
    53521 ataagatgaa gattattgta cctgcaacca gtgccaatat cgggccaggt tttgactcgg
    53581 tcggtgtagc tgtaaccaag tatcttcaaa ttgaggtctg cgaagaacga gatgagtggc
    53641 tgattgaaca ccagattggc aaatggattc cacatgatga gcgtaatctc ttgctcaaaa
    53701 tcgctttgca aattgtacca gacttgcaac caagacgctt gaaaatgacc agtgatgtcc
    53761 ctttggcgcg cggtttgggt tcttccagct cggttatcgt tgctgggatt gaactagcca
    53821 accaactggg tcaactcaac ttatcagacc atgaaaaatt gcagttagcg accaagattg
    53881 aagggcatcc tgacaatgtg gctccagcca tttatggtaa tctcgttatt gcaagttctg
    53941 ttgaagggca agtctctgct atcgtagcag actttccaga gtgtgatttt ctagcttaca
    54001 ttccaaacta tgaattacgt actcgcgaca gccgtagtgt cttgcctaaa aaattatctt
    54061 ataaggaagc tgttgctgca agttctatcg ccaatgtagc ggttgctgcc ttgttggcag
    54121 gagacatggt gaccgctggg caagcaatcg agggagacct cttccatgag cgctatcgtc
    54181 aggacttggt aagagaattt gcgatgatta agcaagtgac caaagaaaat ggggcctatg
    54241 caacctacct ttctggtgct gggccgacag ttatggttct ggcttctcat gacaagatgc
    54301 caacaattaa ggcagaattg gaaaagcaac ctttcaaagg aaaactgcat gacttgagag
    54361 ttgataccca aggtgtccgt gtagaagcaa aataaagaat agaagatagg atggggaaac
    54421 tcttgaccag agggtttcat atcctttttg tgaaaagaag tttatactca atgaaaatca
    54481 aagagcaaac taggaagcta gccgcaggct gctcaaaaca gtgttttgag gttgtggata
    54541 gaactgacga cgtcagctca agacactgtt ttgaggttgt ggatagaact gacgaagtca
    54601 gtaacatata tacggtaagg tgacgctgac gtggtttgaa gagattttcg aagagtatta
    54661 gttaaaaacg tgataaagga gaaataaaga tggcagaaat ttatctagca ggtggttgtt
    54721 tttggggcct agaggaatat ttttcacgca tttctggagt gctagaaacc agtgttggct
    54781 acgctaatgg tcaagtcgaa acgaccaatt accagttgat caaggaaaca gaccatgcag
    54841 aaacggtcca agtgatttac gatgagaagg aagtgtcact cagagagatt ttactttatt
    54901 atttccgagt tatcgatcct ctatctatca atcaacaagg gaatgaccgt ggtcgccaat
    54961 atcgaactgg gatttattat caggatgaag cagatttgcc agctatctac acagtggtac
    55021 aggagcagga acgcatgctg ggtcgaaaga ttgcagtaga agtggagcaa ttacgccact
    55081 acatcctggc tgaagactac caccaagatt atctcaggaa gaatccttca ggttactgtc
    55141 atatcgatgt gaccgatgct gataagccat tgattgatgc agcaaactat gaaaagccta
    55201 gtcaagaggt gttgaaggcc agtctatctg aagagtctta tcgtgtcaca caagaagctg
    55261 ctacagaggc tccatttacc aatgcctatg accaaacctt tgaagagggg atttatgtag
    55321 atattacgac aggtgagcca ctcttttttg ccaaggataa gtttgcttca ggttgtggtt
    55381 ggccaagttt tagccgtccg atttccaaag agttgattca ttattacaag gatctgagcc
    55441 atggaatgga gcgaattgaa gttcgttctc gttcaggcag tgctcacttg ggtcatgttt
    55501 tcacagatgg accgcgggag ttaggcggcc tccgttactg tatcaattct gcttctttac
    55561 gctttgtggc caaggatgag atggaaaaag caggatatgg ctatctattg ccttacttaa
    55621 acaaataaaa cagagagtgg ggcttcccac tttcttcatt tctagaatat gaatagaagg
    55681 gatttatgaa acacctatta tcttacttca aaccctacat caaggaatca attttagccc
    55741 ccttgttcaa gctgttagaa gctgtttttg agctcttggt tcccatggtg attgctggga
    55801 ttgttgacca atctttacct cagggagatc aaggtcatct ctggatgcag attggcctgc
    55861 tccttatctt tgcagtaatt ggcgttttag tggccttgat agctcaattt tactcagcaa
    55921 aggcagcagt aggctttgct aaggaattga caaacgatct ttatcgtcat attctttcct
    55981 tgtccaagga cagcagagac cgtctgacaa cttctagttt ggtcactcgc ttgacttcgg
    56041 atacctacca gattcagact ggtatcaatc aattcctgcg tctctttttg cgagcgccca
    56101 ttatcgtttt tggtgccatt tttatggctt atcgaatctc agctgagttg actttctggt
    56161 tcttagtcat ggttgccatt ttgaccattg tcattgtagg gttatctcga ttggtcaatc
    56221 ctctctacag tagtctcaga aagaaaacgg accaactggt tcaggaaacg cgccagcaat
    56281 tgcaagggat gcgggttatt cgtgcttttg gtcaagaaaa acgagagtta cagatttttc
    56341 aaacccttaa ccaagtttat gctagattac aagaaaagac aggtttctgg tctagtttat
    56401 taacacctct gacctatctg attgtcaatg gaactcttct cgttattatc tggcaaggct
    56461 atatttcaat tcaaggagga gtgctcagtc aaggtgctct cattgctctt atcaattacc
    56521 tcttacagat tttggtggaa ttggtcaagc ttgccatgct gatcaattcc ctcaaccagt
    56581 cctatatctc agccaagaga atcgaggagg tctttgcgga atctcccgaa aacatccatt
    56641 cagaattaga acaaaagcaa gttaccagtg gtcgggtttt acaagtccaa gaattgactt
    56701 ttacctatcc tgatgcggcc cagccttctc tgagagacat ttcctttgat atgactcaag
    56761 gacaaatcct tggtatcatt ggggggactg gttctggtaa atcaagcttg gtgcaactct
    56821 tacttggact ttatccagta gacaagggga acattgacct ttatcaaaat ggacgtagtc
    56881 ctcttaattt ggagcagtgg cggtcttgga ttgcctatgt acctcaaaag gtcgaactct
    56941 ttaaaggaac cattcgttcc aacttgactc taggtttcaa tcaagaagta tctgaccagg
    57001 aactctggca ggccttggag attgcgcaag ctaaggattt tgtcagtgaa aaggaaggac
    57061 tcttggatgc tctagttgag gcaggggggc gaaatttctc aggtggacaa aaacaaagat
    57121 tgtctatcgc ccgagcagtc ttgcgccaag ctccgtttct catcctagat gatgcaacct
    57181 cggcactgga taccattaca gagtccaagc tcttgaaagc tattagagaa aattttccaa
    57241 acacgagctt aattttgatc tctcaacgaa cctcaacttt acagatggcg gaccagattc
    57301 tcctcttgga aaaaggtgag ttgctagctg ttggcaagca cgatgacttg atgaaatcca
    57361 gccaagtcta ttgtgaaatc aatgcatccc aacatggaaa ggaggactag aatgaaacga
    57421 caaactgtaa accagacgct caaacgttta gccgtagatt tagcaagcca tcctttcctc
    57481 cttttcctag cctttctagg aactattgcc caagttggct tatcaattta cctacctatt
    57541 ctgattgggc gggtcattga ccaagtccta gtggctggtt catcaccagt tttttggcag
    57601 atttttctcc agatgctctt ggtggtaata ggaaatactc tagtacaatg ggccaatcct
    57661 ctcctctata atcgtctaat cttctcttat accagagatt tacgggagcg aatcatccat
    57721 aaactccatc ggttaccgat tgcctttgta gataggcaag gtagtggaga gatggttagt
    57781 cgtgtaacca cagacatcga acagttggca gctggcttga ccatgatttt taaccaattt
    57841 ttcattggtg ttttgatgat tttggttagt attctagcca tgctccaaat tcatctcctc
    57901 atgactctct tagtcttgct gttgacgcca ctgtccatgg tgatttcacg ctttattgcc
    57961 aagagatcct atcatctctt ccagaagcaa acagagacga gggggattca gacccagttg
    58021 attgaagaat cgcttagcca gcagactata atccagtcct tcaatgctca aacagagttt
    58081 atccaaagat tgcgtgaggc tcatgacaac tactcaggct attctcagtc agccatcttt
    58141 tattcttcaa cggtcaatcc ttcgactcgc tttgtaaatg cactcattta tgccctttta
    58201 gctggagtag gagcttatcg tatcatgatg ggttcaacct tgaccgtcgg tcgtttagtg
    58261 acttttttga actatgttca gcaatacacc aagcccttta acgatatttc ttcagtgcta
    58321 gctgagttgc aaagtgctct ggcttgcgta gagcgtatct atggagtctt agatagccct
    58381 gaagtggctg aaacaggtaa ggaagtcttg accagtgacc aagttaagag agctatttcc
    58441 tttaaacatg tctcttttgg ctaccatcct gaaaaaattt tgattaagga cttgtctatc
    58501 gatattccag ctggtagtaa ggtagccatc gttggtccga caggtgctgg aaaatcaact
    58561 cttatcaatc tccttatgcg tttttatccc attagctcgg gagatatctt gctggatggg
    58621 caatccattt atgattatac acgagtatca ttgagacagc agtttggtat ggtgcttcaa
    58681 gaaacctggc tcacacaagg gaccattcat gataatattg cctttggcaa tcctgaagcc
    58741 agtcgagagc aagtaattgc tgctgccaaa gcagctaatg cagacttttt catccaacag
    58801 tttccacagg gatacgatac caagttggaa aatgctggag aatctctctc tgtcggccaa
    58861 gctcagctct tgaccatagc ccgagtcttt ctggctattc caaagattct tatcttagac
    58921 gaggcaactt cttccattga tacacggaca gaagtgctgg tacaggatgc ctttgcaaaa
    58981 ctcatgaagg gccgcacaag cttcatcatt gctcaccgtt tgtcaaccat tcaggatgcg
    59041 gatttaattc ttgtcttagt agatggtgat attgttgaat atggtaacca tcaagaactc
    59101 atggatagaa agggtaagta ttaccaaatg caaaaagctg cggcttttag ttctgaataa
    59161 gcgattctct tttgaaagtt tatggacgaa aaaagttgcc ttcgagtgac ttttttgtta
    59221 caatagctag aaaaattgtt cactgtaata ctcaatgaaa atcaaagagc aaactaggaa
    59281 gctagccgta ggttgctcaa agcacagctt tgaggttgta gataagactg acgaagtcag
    59341 ctcaaaacac tgttttgagg ttgcagatag aactgacgaa gtcagtaacc acacctacgg
    59401 caaagtgaat ctgaagtggt ttgaagagag tacaacttgt cttttagaaa aggagcctac
    59461 aatgaaagtc tttcagcatg taaatatcgt gacttgtgat caaaatttcc atgtttatct
    59521 tgatggaatc ttagcagtca aggattctca aatcgtctat gtcggtcaag ataagccagc
    59581 gtttttagaa caagctgagc agattataga ctatcaggga gcttggatta tgcctggttt
    59641 ggtcaattgt cacacccatt ctgcaatgac aggtctgaga gggatccgag atgacagcaa
    59701 tctccatgaa tggctcaatg actatatctg gccagcagaa tctgagttta ctcccgacat
    59761 gactaccaat gcggtcaaag aagccttgac agagatgctc cagtcaggaa caacaacctt
    59821 taacgatatg tataatccca gtggtgtgga tatccagcaa atttatcagg tggtgaaaac
    59881 ttccaagatg cgttgttatt tttctccgac tctcttttct tcagagacag aaacaactgc
    59941 tgagactata agcagaactc gatccatcat agatgaaatc ttaaaatata aaaatccaaa
    60001 tttcaaggtt atggtagcac ctcattctcc gtatagctgc agtagagact tgctggaagc
    60061 gagtttggaa atggcaaaag agctaaatat tccgctccat gtccatgtgg cggagaccaa
    60121 ggaggagtca ggaattatcc tcaaacgata cggcaaacgc ccccttgctt ttcttgaaga
    60181 actgggttat ttagatcatc cgtccgtatt tgctcacggg gtcgaattaa acgagagaga
    60241 aattgaacgc ttggcatctt ctcaagtggc tatcgcccac aatcctatca gtaacctcaa
    60301 actggcatca ggaattgctc caattatcca gctccaaaaa gcgggagtag cagtcggaat
    60361 tgcgactgac tcggttgctt ccaataacaa tctagatatg tttgaggaag gaaggactgc
    60421 agctcttctt cagaagatga aaagtgggga tgccagccag tttccaatcg aaacagctct
    60481 caaggtactg acaatcgaag gggctaaggt ccttggaatg gaaaatcaga taggaagtct
    60541 ggaagtcggc aagcaagcag attttctggt cattcaacca caagggaaaa ttcatctcca
    60601 acctcaggaa aatatgctgt ctcacctggt ttatgcagtt aaatctagtg atgtagatga
    60661 tgtttatatc gccggagaac aggttgttaa gcaaggtcaa gtcctgacag tagaacttta
    60721 aaagaaaaat tacgaaaaat tttaaaaaaa gtttgcaaaa atcttgcatt ctttttttga
    60781 ctatgctata cttatatacg gtttaaaaaa actgcctaag acagtagggg agctcgactc
    60841 ataaatatcc taccgaggac aaaacgtatc atgtaaaaag aagcgtattg tactttcgtg
    60901 tctaggtttg ggcgcgtttt tctttttgaa aaattcccca agcaaaataa ttacggaggt
    60961 gaacacacta atgagtgaag caattattgc taaaaaagcg gaactagttg acgtagtagc
    61021 tgaaaaaatg aaagctgctg catctatcgt cgttgtagac gctcgtggtt tgacagttga
    61081 gcaagataca gttcttcgtc gtgagcttcg tggaagcgaa gttgagtata aagttattaa
    61141 aaactcaatc ttgcgtcgtg cagctgaaaa agctggtctt gaagatcttg catctgtatt
    61201 tgttggacca tctgcagtag cattttctaa tgaagatgtt atcgcaccag cgaaaatctt
    61261 gaacgacttt tctaaaaacg ctgaagcact tgaaattaaa ggtggtgcaa tcgaaggcgc
    61321 tgtcgcatct aaagaagaga ttcttgcact tgcaactctt ccaaaccgcg aaggacttct
    61381 ttctatgctc ctttctgtac ttcaagcgcc agtgcgcaac gttgctcttg cagtcaaagc
    61441 ggttgcagaa agcaaagaag acgcggctta atcttaagct acacagcgta gcctagctac
    61501 gaaaaaaact attataaaat ttaaaactta tttggaggaa ataacaatgg cattgaacat
    61561 tgaaaacatt attgctgaaa ttaaagaagc ttcaatcctt gaattgaacg accttgtaaa
    61621 agctatcgaa gaagaatttg gtgtaactgc agctgctcct gtagctgttg ctgcagctga
    61681 tgcagctgat gctggtgctg ctaaagattc attcgacgtt gaattgacat ctgcaggcga
    61741 caaaaaagtt ggcgttatca aagttgtacg tgaaatcact ggtcttggtc ttaaagaagc
    61801 taaagaactt gttgacggtg caccagcact tgttaaagaa ggcgttgcaa ctgcagaagc
    61861 tgaagaaatc aaagctaaat tggaagaagc tggagcttca gttactctta aataatagag
    61921 tatcgcaatt tgattgcgga atccctattt accagtcttt gagagcgata agcgattgat
    61981 agaaactgat aaattgattt ggtttcctgc caaaaattct ttggcaggat ttttgtttta
    62041 ataaactttc gggggtagac actagattgt ctaccccttt ttttgtgttt cggaggtaat
    62101 tatgcaaaag gaaggacagt catcacgtca attcgtgaag agtcatctca tgactatcat
    62161 gggagttgat attgtaaaag caactcagtt agttgatgaa atggaacaag taggtttaat
    62221 ccgatttgat gaatttggaa atgttggaat tttagtcttg gagggacaat aatgaagcgt
    62281 attaccgcta atcaatatca gacatcagag cgttattata agctccccaa acttttattt
    62341 gagagtgaac ggtataagaa tatgaaactg gaggttaagg tggtttactc agttttaaaa
    62401 gatcggttgg agttgtcttt gagcaaaggt tggattgatg aggatggggc tatttatttg
    62461 atttattcca attcaaattt gatggcactt ttaggctgtt caaagtcaaa attactctcc
    62521 atgtgagttt gaagtgacat ttttagatga ttaccataaa aaacataact acccactatt
    62581 ttacgaatcc tatcttcaaa acgttatgga attccttgaa agtcaagaca taaagaatgg
    62641 ggttgatgcc tttgtagatg atcatcaaaa tctcgttttt gttttatatg gacaaggcta
    62701 tcgagccgag ggaaaagagg gaatacttac aacccaagta actgtaaaag cttatgatga
    62761 agacaagaaa ccgattaact tcgcaaattt attagattcc ttaatttatt agattcctta
    62821 atcgtgtcag aatatcaaat ggaaccgaat ctttgggagg tctcccatga ttgatctcta
    62881 tctaagtaaa aatagacaaa gaaatcaact tcttttagat ttcttccaaa actatggcat
    62941 agaggtatct tgtcattcag tttctgaaat gacaaaggac aaattaattg agatgatgag
    63001 ctattcttca gattgttttg agtttttatc tcccaattta ttacgattta agaaccgtga
    63061 caatctaaga ttaacggatt tcgttgagat aattttaaaa aatcctgaac taaccatcag
    63121 acttcctctt gcggtttcaa ataagcgagt ttatccaagt ctgaatttgg aagaggctag
    63181 agctctattg ccaagagata cgaaacaatt gatttacatg gcgcaaacac attatttatc
    63241 taactaaagg agaagcaata tggctgaacg attttgggag aacttgtcca ttattttggc
    63301 tgaaagaaat atcagctgga ttgagttaac cagaaaaatg tttgcgggag agtttcacta
    63361 tccaagtgaa ttgaatcgtt tgtatcaaaa gattcgccac tagaagatgg aacaatgaat
    63421 gcctcaaagt ccatgggtag aaaggattgt gcaggtatta gatttagatt atgaagattt
    63481 atttcggagg taactatgaa aagaataatt ccagtttata tattccaaca agtaaatgtc
    63541 ctattggtat ctctatactt attgaaattt ctttgtatcg gtgagttaac tatactacag
    63601 attctctatg gttcgtcact catttctttt ttatggatgt atggccaacg caaacaagcg
    63661 cataaggtca atatgaaatc taggatgaaa tggcttggtg ttgaatttgt tagcctactg
    63721 attataagtc tatgttttag tctgattcat gctcaaggaa gcacgaatca agcaaactta
    63781 attggccttc aacatcaaat tccttggttt tcatttttat tgttcttaat caatgcgagt
    63841 atggttgaag aatttctgta tcgagaaatt ttatggaatt tggtcagaaa attagatatc
    63901 agagttgctt tgacaagtgt tttatttgcc ttagcacatc atacaggaac cattctagct
    63961 tggtgtttgt atgtttcact tgggatgttt ttaggtatgg tgcgctataa atctgactta
    64021 tggggcagta tgggactaca tttggtgtgg aacctactag tttatagttt gctgcttttt
    64081 taaaataggt gacctgattt ttaggtaact tctaaaacta acttccagtt ttgggagaga
    64141 gatggaagtt actttgaaaa gttacggaaa cattattttc tatgtgacaa cattgtataa
    64201 ttgcttgcat tttactggtg gagagagtat aatgagggtg gataaagtac ttaatcccaa
    64261 aaatttaaat aggaggagct agctatggca gaagaagtat taaatcttca attagtatct
    64321 gttcaagttg acgaaacaga tgaagttgat ggaatgcgat ttagtacatt cagtacaaat
    64381 cgttgcggta actggagtgc ttttagttgg gaaaattgtt aattcattta aattgtaaac
    64441 tttagagatt ctctggagtt atggatgtcg ttccaatatg tgcacgttgg aatgttagtg
    64501 cttatatggg gagaatccaa aggattctcc ctttttattt aaataagtct gtctagctat
    64561 agatatatta agtttgataa aatttcttgg agattttgtt atggattata atttcaattt
    64621 ggagcatcct ttctttttca ctaataatga ttatagtaca gatactagca taaagtacca
    64681 agtaagtctg ccttttaatt ggcacgaagt aatgaataat gatgagtggg tttatcaata
    64741 ccctattgga aaatttgttg aaagacaagg gtggaaaata cacattagtt cagagtataa
    64801 cagttcacat gaattgttac aagatgtagc aaaaatttgc catgaaatgc gaattccatt
    64861 taaacatctt agtacagaag acaaatttat catgcgaaac gggaaattgg ttagtcgagg
    64921 attttctggt aaatttatta catgttatcc aaatcaaaat gaattagagt cggttttgca
    64981 aaggttagaa agtgccttaa agcaatataa tgggccatat attttaagcg ataaacgttg
    65041 ggatgaagca ccaatatatc tgaggtatgg tgtgtttagg ccgtcaaggg atgatgaaaa
    65101 gaaggttgct attgatgaat taattgttgg cgatgaagtt gttaaagatg aacgtttacc
    65161 agtatttaaa attccaaaag gtatagtgcc ccctgacttt ttgaataaat ggcttgacaa
    65221 gaaagataaa aaacagggag actttccctt tattatagat aatgcaatta gattttcaaa
    65281 cagtggtggg atatataatg ctaggctaaa agaagatggg aaaaaaataa ttttaaaaga
    65341 ggctagaccg tatacaggtc taggatttga tggtacttat tcttcagaga aattagcttc
    65401 ggagtgcaaa gcactaaaaa ttctaaatga atggagtgag acgcctaaaa tatactggca
    65461 cggtaagatc tgggagcaca ccttcttagg aattgaacat atgaagggcg ttccactaaa
    65521 tagatgggtt accaataatt ttcctttata tgaagttgtt gataagacca aagattatct
    65581 attgagagtt tcaaagatag tagaaaaact catcgattta acgaataagt ttcattctga
    65641 aaatgtatat catcaagatt tacatttagg aaatatcttg gttaaggatg aagatgagat
    65701 tagtataata gactgggaac aggctgtctt ttcaaatgat gaaaaagttg tacataaagt
    65761 tgcagcccct ggttttagag catggcgaga aacacttccc agtgagatag attggtatgg
    65821 tattagacaa atagctcatt atttatacat gccactagta actacatcgg atttgacata
    65881 caactatgta agtcaaacca ggatagaagg aaaaaaacta tttgagtctt tgggttatac
    65941 aagagaacat atagattatg tagaaagttt attgtcatat ctagattcta aatgtccaca
    66001 gattgagaat atttccagga aaaaagtttt aaaacccatg catgagatta gaactatcga
    66061 gagtgagcaa gatatacaag attttattat aaaattattg agagggttta cgctaactta
    66121 tggacagtgg cgtaaagaat ttcaatcaag attcttccca gttcattatt atggtctgaa
    66181 ttttaatcaa ggaattgcgt tttcagattt agctatcctt tggagttatc aacaactagc
    66241 taagaaagta aaaaatttca agtttgatga ctattatgag attcgaacac aggttattaa
    66301 tgaggcagta aataatttta agaaatcgtc cctttctggt ctttttgatg gtaaaattgg
    66361 tacaatctgg ctcatttatg aatttgggga gatagatcgg gcagttgaac tttttacaac
    66421 tcattttata gagatatttg agaattcaca aaataaaaat ttatatagtg ggcaggctgg
    66481 aattttactg gttggactgt attttcttag taaaggggaa attgataata aactaggaga
    66541 agaaatacta attaggctta gagaatatac tttaaattat atagaaaatc cagaaacttt
    66601 ttgtaaagtt ggcgctagtg atgttcagag taatgatcct tatgagaatt ttggtggatt
    66661 actatatggt cacgcagggg tagcttggct cttcggtgaa gcttataaat taactggtga
    66721 aagtatctat aaaaatggtc tagaattggc tgtagataaa gaattagttg cttataaagt
    66781 agatagtaat aattcattgc agtacagcca aggtcataga ttactaccat atctagcaac
    66841 tggaagtgct ggcctgcttt tacttattaa tagaaataaa gaaatcttgt caagcaaata
    66901 cttaaaatat ttaacttctt tagagagggc aactgatgta gttttttgtg tattaccagg
    66961 tttatttaac ggattttgtg gtcttgaagt tgctaataat atatatagtg atatcgatga
    67021 taacttttca ggacaaaaga aattaattga acaactttat agatatttgt gtgtaattga
    67081 agaaggattt gttattgcgg gagataatgg tttaaagata acaacagata ttgcttcggg
    67141 atttgcgggt gttgcaattg gattggtatc aataatggat aataaattga cgattcttcc
    67201 tcaaatataa tatttagata ccattattaa ggggtcatac ttgtgaatca gagtaatgaa
    67261 atatttttaa atacaataca ggaaaaaaca cactataaga ggtctatctc ttcatcattt
    67321 caggaattaa aaaaatggaa ttctttgata tttaggagag ggcgatattg gtaccaattt
    67381 gataattgga gtatacccaa tactggtgta ataaaggaat ggtatgtcga agataatgac
    67441 tggattccta aggttagttc ttccagttta gacaattcaa attcacttaa aattgtcttt
    67501 gatgtggaaa aatatacttt gccagatgat agtatcttac aggatgttag gatttcatct
    67561 gatgagagtt atatagtttt ggttgtatcc tcactgcgta gcactaattt aattggaata
    67621 aaaaaatata ctatggaaga gttatttatc attgaagata tttcagtaga aagttcgttc
    67681 tatttgggta aattcggtgt tatgtatact cgctccaaag agtacgggag gccaagtaag
    67741 ttattctata aatcttttga tagctttaca gaagaggaac tgtttgaaga aaacgaatgc
    67801 tcttttcgat taaaaatagt ccacattgat agcaataatt gttttgtaaa gtcagtagac
    67861 tttcagaaag gcaggatttt tctatacagt ttcgaccgga cgggttttgt tagacactct
    67921 tacacagaaa cggtagcccc aacacctaga gatatcgctc tatttagtac agaaaaagca
    67981 cagtactttc ttgggctctc ttcaactgaa gaaaagaaag atcaactaat tctaagggaa
    68041 acttcttcgg gagacagagt ttctattact ataccatatc aagatagggc tagaagagtt
    68101 cactgtatag gacgttatat ccttttagac tgttcaaacg cagcgaattc cgtcttttat
    68161 cttgctacat ttaaaaataa tagtttgagg gagttgagtg ttaataagat aacatttgat
    68221 gaaaagacaa cactttatga aaattcattt tctgatcgtg ttcttctgtt tttagagagg
    68281 agaacttttt acgaaaaaat acttagtttt gatttaatag aaaatacatt aaaaactgaa
    68341 tttgaaagac ccatcataaa atcgaataat actaaatatt tttctaaggt gatttggact
    68401 aaaaaagaca gttatgatgt aagcataccg atttctttat tttggaaaag tgaagatacg
    68461 gatgagctcc caagaagaaa aaaatgtatt ctaagtgtct atggggctta tggtaagaat
    68521 gataattctg atttagatga aattatgtta tctataatag atgcaggttt tatttatgct
    68581 atagttcatg taagaggtgg aggctatttg ggtggtgaat ggtatcgctc aggaaaggcg
    68641 ttaaacaaat ggaattctat cagagatttt attgagggag taaattattt aagggaaaat
    68701 gatgtaattg acagtaagcg attaggttta ataacttcta gtgcaggtgg aataattgct
    68761 ggtgcggtgt tgaacgagga aaaaaattta ttgcaaagca ttctcttatt ttcgccattt
    68821 ataaatcctt atgatacact tcagaatcca aatgatcctc tttctaagac cgaaatagca
    68881 gagtggggag atattagaga ccctgaagta aaggcttata taaagtcata ttcgcctatg
    68941 caaaatattg aaaaggcacg agactcaaat actgttatag ttaatatttt gggtgagaaa
    69001 gacccatata ttaataataa cgaagtgata gagtggtcaa agaaattaaa ttctattgga
    69061 gttaaaagtt tgttgtattt aaacaaagca gctggtcatg gaggttttac tccatcagat
    69121 gttctcttaa tgattgatac tttaaattat ttctttgaag aagtgggaag gaataactta
    69181 tgaattattt aaaatttata aaaaagacta aacttatatt aatggggatt ttaatatttc
    69241 tatcttcgtt taatggtgtc ttgctctcag gaattattgt atatgcaggt agtttaaatc
    69301 agacttcctc attttctgac gttctaagat ttggtgctat aagtattctg ggttggtcag
    69361 caatctatat atcaaattac tatttagaag taacggaagc atcaataacg aaagatataa
    69421 atgtaaaaat taaacaagga tattttagag aacagtatct ttcttctgaa atggttaaag
    69481 attactcatc tattatttca gttttgtcaa atgatttgag attaatcgaa gaaaactatt
    69541 ttagacaaat ttttgaaata atttcttcaa tattgttgtt catcgtctct ctaagtttta
    69601 tgctttattt aaacttccta gtttcaataa tatttattgt attatcagca ttgccgatta
    69661 tagtacctgt ctttatgaag aagatgttgt ctaattcagc taatgagtac tctaacagca
    69721 atgcagagta tactcacata ataaaagaaa tttttaatgg ttttaagaca ttaaaatctt
    69781 attctgttac taaagaaata attagtttgt cggataaaaa gttggataaa ctagaagatt
    69841 ctacttttaa tttgaaacga tcagaggttc tttcaaaatt ggttgcagta ctaatttcag
    69901 gtttttgctt tctagttccc ttggttgtag gatgttattt tgtaatttat cataaaagcc
    69961 tttcttttag tgaactgata ggtattttct tagcaaacga taaagtttta gggccgatac
    70021 aatcaattgc ctattcatta aataagataa atacaaccaa agatttaagg aaaccgtttt
    70081 taaaatactt aagtggagag aagaatttta tagacgctga acatgataat aacggactgt
    70141 atacttcatc aatagatgag atacacatga aagatgttgt atattctatt acaccagaaa
    70201 ataaattaag tattgacttc tcatttaagt caccatttag ggtattatta acaggaactt
    70261 ctggtagtgg gaaaacaacg attttaaatt taattaatgg ttctttaaag tcacaaaaag
    70321 gttatgtaaa tttgttatca catgggaaaa agagttcaga ttcaatacca acagttgatc
    70381 agacaccata tatttttgac actactattc gtgagaacgt aactttattt caaaatgaat
    70441 atttttcaga tgatcagata attgaggtgt taaaaaaggt aaatctatat gaagaattag
    70501 aaaagataga tatactaaat tatcaatgtg gtgaaaatgg tagtaatttg tctggaggtc
    70561 aaaaacaaaa aatagcttta gctagagctc tgattagaaa taataaagtg tacttatttg
    70621 acgaaatatc agctaattta gataatgata attcaaattc catacatgat attctgttca
    70681 atttaggtat ttcatttatt gaagtttcac atcattatga cttaaatgac aagagataca
    70741 ctgatatata taaattggaa aatggaacac ttttcaaaat taagtgattg gaggcaagaa
    70801 tatggataaa gattatatat taaaagtgaa agggctgtat catcaatttt tactaggaaa
    70861 taataaaacg ttgcaagtgc tgaaaaatgt ttctctttct gcttcgagag gagaatttat
    70921 aagtattcta ggaattagtg gttctggaaa gtcaacttta ttaaaatgta tttctagttt
    70981 gcttgaacca acaagtgggg aagtaatttt aaatggaatc aacccctata aaatcagaaa
    71041 tgcaaaattg tcaagtataa gacgtaacga agtatctttt atatttcaag catacaattt
    71101 aataccttcc ctgccggtaa tagaaaatat agcacttcct ttgcgattat cacaaaaaaa
    71161 attaactatt aaaaatgtag aaaacttact caaaagaatg aagtttaatg ctggcttaaa
    71221 cgattttgtt ggaactctgt ctggtggaga acaacagaag gttgctatag ctagagcggt
    71281 tattgctgat agtgatataa tatttgctga tgagccaact ggggctttag acagcgtttc
    71341 tcgtgaagta atttttgaat tattgagaga gttagtaggg gcgggtaagt gtgtaattat
    71401 ggtaacgcac gatatagaat tggcctcgaa aactgatcgt gcattaatat tgaaagatgg
    71461 aaaaattttc aaagaacttc atagacctag cggggaagag ttgtataaaa tcttagaggt
    71521 acaatcaact acggaggaat agagatgctc agattagttt actatcaatt tttacataat
    71581 aagaaacaat ggttaggcgt ttcaccagta atttttgttt cgagtttagt tatgggattg
    71641 gctgttaatg gggtaattaa tgttgagaat aattcgcaag tatttgtcgg acttccagat
    71701 cctaaaccaa tttttatgtt tccgatagta ttcggaggag taacattatt ctttgtgctc
    71761 tctaacataa taaatatgtt ggttgaaatt tttagagatg attacgaact tctggaggtg
    71821 ttgggagcaa gtctccttca actatcattt cttgtggggg gacagatttt tatcatttcc
    71881 tcgattatta gttttattgc atatctatgc tctatatttg ttacgtcaaa ttattattat
    71941 tttttacaat actttttcgg ggaaaatata ttacctgata tccaatttca aacgagtgct
    72001 gttggatgta tcattacagt agtgttgatt tcatttttag cctttctaag tggttgcttt
    72061 tatacgttca agaagattag aaataggaag agttctaaaa taaggcatgt tctttcaata
    72121 gtaaaaagaa tactgctatt ggctggtttt agtgttatct ggttgctttc tttgcaacaa
    72181 attttccaag atagcacaat tttagcgaag gcccagataa tattcaatat tgtaatctta
    72241 gatattgtga ttatttatca attatctcct tttatacagt catgttttat caaactactt
    72301 tctataatta tatttagaaa taattttatg ttcattgttt cgaagtggaa tttactatat
    72361 cgtaagcctt atataaaatc tatcagtgct gcaattacag gagctatatt actaatttct
    72421 agttttcaaa tgatttctca gaatatacta tctcaatttc aagatgacag tgatttagag
    72481 ctaaaagtgg catttattgt ctatgtagga gcaccaattt tgatagtact tgcaaatatt
    72541 atttcaattg catttttgtc atcgcatcaa gaaaggattg agattcagca atttgaaatt
    72601 ttaggaactt ctaattacca gatggttaag attaaagttg gtgaggctat atttttgaca
    72661 tttgttactt cattaattgc atttttactt aacattaaaa ttatggcttt aatatactac
    72721 tctttagagg atattttaat agatgatatg aatcttttgg ggtta
//
LOCUS       DBEROU010000004        68417 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.004, whole genome shotgun sequence.
ACCESSION   DBEROU010000004 DBEROU010000000
VERSION     DBEROU010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 68417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68417
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.004"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            137..1567
                     /locus_tag="ACL8CL_000268"
     CDS             137..1567
                     /locus_tag="ACL8CL_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGS1329583.1"
                     /translation="MQILISEVNVTSFGITREILKKMDFNFSKMGSEEAKFLIFPELP
                     RGGELSLTARQSYDSQLRYSMLPRVEDIMKKIVNQLREHLKILDIELSDLSYLSEGDQ
                     FILKELSSHVKINIKITPIKKIRYVYEDIFLNQSAEELHELGLKYLSAGDLWASKKIF
                     TYLVNKYDNRRDVFKLATTYNMLLETEKAEYYYNLYKHDGKVDHIVAANYMLAMLYAR
                     HHPKYLQDDQKAIVLLNEAYDLIKDKVTLESIFNRNGYALLLFKMGKVEETIILLKRC
                     LNQIKQLDNSSSSVVLHESVLWFNLYQCYVTIGDNTNSRKTFYILKNMDPLFIEYYLE
                     YSRNLLETDVENFLEFIESMPHHSNNFAEKYSLLGYYFRGTAKSLEYFSKAFELSNRT
                     FSFWYDITYELTEFNLYNEAYNNLPLLNNKLYELNDEDLLNYILISAEIFYNLDRLDE
                     FKRTIEIGYRIFPDSESIKEIIENAE"
     gene            1564..2739
                     /locus_tag="ACL8CL_000269"
     CDS             1564..2739
                     /locus_tag="ACL8CL_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1329584.1"
                     /translation="MKRYKTLFIKYPDSFQFLVFNILFYLSMSLSRSVHSIWFDLNGS
                     LLNYSISYSAMAFSGMFAGILGRRINYFNLRNLIRIGVILYSIGLLLRLFPNDWLISL
                     ISGLISGIGAATIILLLRTWAVGIGKEKDRVVTISLKRFSSSLGTSIGGILSGILLYC
                     LIYFFHSMAYPIVLASASMLCLVTFFCIPKIDSFVDKSERAERILESSNYMKKRIIFF
                     GIFSGLIASIFSPYLPVILRRQEISEVFIGPVLTILSLLTIYFSSIFSSRKVDNHKQG
                     YYILSELLISILTLLFSFHLKYYLVITILIIRVFFVNLSSITQELMELSIYPKEKISY
                     FYGISQTSYFIGDTLGGFIGGWLFNQDMKYSIYLFSVLLLFNAVLFNRFYLRNNKNS"
     gene            complement(2873..3930)
                     /locus_tag="ACL8CL_000270"
                     /pseudo
     CDS             complement(2873..3930)
                     /locus_tag="ACL8CL_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681261.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4501..6450
                     /gene="mutL"
                     /locus_tag="ACL8CL_000271"
     CDS             4501..6450
                     /gene="mutL"
                     /locus_tag="ACL8CL_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HGS1329585.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGYGISHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGYGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(6775..7242)
                     /gene="ribE"
                     /locus_tag="ACL8CL_000272"
     CDS             complement(6775..7242)
                     /gene="ribE"
                     /locus_tag="ACL8CL_000272"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HGS1329586.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(7243..8448)
                     /locus_tag="ACL8CL_000273"
     CDS             complement(7243..8448)
                     /locus_tag="ACL8CL_000273"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HGS1329587.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(8468..9103)
                     /gene="ribE"
                     /locus_tag="ACL8CL_000274"
     CDS             complement(8468..9103)
                     /gene="ribE"
                     /locus_tag="ACL8CL_000274"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HGS1329588.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(9088..10188)
                     /gene="ribD"
                     /locus_tag="ACL8CL_000275"
     CDS             complement(9088..10188)
                     /gene="ribD"
                     /locus_tag="ACL8CL_000275"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HGS1329589.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(10323..10437)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            10594..11187
                     /gene="ruvA"
                     /locus_tag="ACL8CL_000276"
     CDS             10594..11187
                     /gene="ruvA"
                     /locus_tag="ACL8CL_000276"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HGS1329590.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDGLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            11197..11760
                     /locus_tag="ACL8CL_000277"
     CDS             11197..11760
                     /locus_tag="ACL8CL_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HGS1329591.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTETELEAMLENPAIIRNRAKLFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKTVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWRGLK"
     gene            11757..12434
                     /locus_tag="ACL8CL_000278"
     CDS             11757..12434
                     /locus_tag="ACL8CL_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1329592.1"
                     /translation="MMSNKNKGILIFAILYTVLFMFDGVKLLASLMPSAIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVGL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(12589..13620)
                     /locus_tag="ACL8CL_000279"
     CDS             complement(12589..13620)
                     /locus_tag="ACL8CL_000279"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S66 peptidase family protein"
                     /protein_id="HGS1329593.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGRAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETSEEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDSNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(13875..14558)
                     /locus_tag="ACL8CL_000280"
     CDS             complement(13875..14558)
                     /locus_tag="ACL8CL_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HGS1329594.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(14570..15514)
                     /locus_tag="ACL8CL_000281"
     CDS             complement(14570..15514)
                     /locus_tag="ACL8CL_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HGS1329595.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            15646..18477
                     /gene="uvrA"
                     /locus_tag="ACL8CL_000282"
     CDS             15646..18477
                     /gene="uvrA"
                     /locus_tag="ACL8CL_000282"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HGS1329596.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPERQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLHEGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVTGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDINQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDAGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            18470..19531
                     /locus_tag="ACL8CL_000283"
     CDS             18470..19531
                     /locus_tag="ACL8CL_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGS1329597.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMNLTRIGFEDEISVSYY
                     HRMQAAFAGLNLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKVGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            19663..20061
                     /gene="spx"
                     /locus_tag="ACL8CL_000284"
     CDS             19663..20061
                     /gene="spx"
                     /locus_tag="ACL8CL_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HGS1329598.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            20127..20696
                     /locus_tag="ACL8CL_000285"
     CDS             20127..20696
                     /locus_tag="ACL8CL_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP0191 family lipoprotein"
                     /protein_id="HGS1329599.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSKYISDRLANGATEQ"
     gene            20783..21049
                     /locus_tag="ACL8CL_000286"
     CDS             20783..21049
                     /locus_tag="ACL8CL_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HGS1329600.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            21053..21472
                     /gene="ruvX"
                     /locus_tag="ACL8CL_000287"
     CDS             21053..21472
                     /gene="ruvX"
                     /locus_tag="ACL8CL_000287"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HGS1329601.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGKFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            21488..21793
                     /locus_tag="ACL8CL_000288"
     CDS             21488..21793
                     /locus_tag="ACL8CL_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HGS1329602.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            22151..23401
                     /locus_tag="ACL8CL_000289"
     CDS             22151..23401
                     /locus_tag="ACL8CL_000289"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGS1329603.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLADQVKE
                     MEKTLLQTPDQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLICQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLDIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGSLKRKD
                     YQGMLGYLTENLPQVELKMTGFDYQGSLDETDVTGYHVIPSYREFISSFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            23485..23916
                     /locus_tag="ACL8CL_000290"
     CDS             23485..23916
                     /locus_tag="ACL8CL_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0198 family lipoprotein"
                     /protein_id="HGS1329604.1"
                     /translation="MKLKRFTLSLASLASFSLLVACSQRAQQVQQPAQQNTNTANAGG
                     NQNQAAPVQNQPVAQPTDIDGTYTGQDDGDRITLVVTGTTGTWTELESDGDQKVKQVT
                     FDSANQRMIIGDDVKIYTVNGNQIVVDDMDRDPSDQIVLTK"
     gene            24062..25594
                     /gene="cls"
                     /locus_tag="ACL8CL_000291"
     CDS             24062..25594
                     /gene="cls"
                     /locus_tag="ACL8CL_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HGS1329605.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPYIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            25672..25815
                     /locus_tag="ACL8CL_000292"
     CDS             25672..25815
                     /locus_tag="ACL8CL_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329606.1"
                     /translation="MLTKEEVNDMIEFKQTHLHRFREIETFVKLCKKSLKQPSQADNP
                     RTF"
     gene            25852..27390
                     /locus_tag="ACL8CL_000293"
     CDS             25852..27390
                     /locus_tag="ACL8CL_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HGS1329607.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSHDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFVHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            27510..29717
                     /gene="nrdD"
                     /locus_tag="ACL8CL_000294"
     CDS             27510..29717
                     /gene="nrdD"
                     /locus_tag="ACL8CL_000294"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HGS1329608.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLVDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            29729..29869
                     /locus_tag="ACL8CL_000295"
     CDS             29729..29869
                     /locus_tag="ACL8CL_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329609.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKYSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            29913..30419
                     /locus_tag="ACL8CL_000296"
     CDS             29913..30419
                     /locus_tag="ACL8CL_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1329610.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKSQSAHDGGFWDTENFVYEEWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDAHNGVER
                     YWIEVANE"
     gene            30412..31002
                     /gene="nrdG"
                     /locus_tag="ACL8CL_000297"
     CDS             30412..31002
                     /gene="nrdG"
                     /locus_tag="ACL8CL_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HGS1329611.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVSGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELSDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            30999..31616
                     /locus_tag="ACL8CL_000298"
     CDS             30999..31616
                     /locus_tag="ACL8CL_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGS1329612.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTSAQELYQALD
                     STTVNLLETDPYITSGRHLVVPKDAPNQKVTAGLPVAHELESLQRDILALQASMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            31882..32190
                     /gene="rpsJ"
                     /locus_tag="ACL8CL_000299"
     CDS             31882..32190
                     /gene="rpsJ"
                     /locus_tag="ACL8CL_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HGS1329613.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            32407..33033
                     /gene="rplC"
                     /locus_tag="ACL8CL_000300"
     CDS             32407..33033
                     /gene="rplC"
                     /locus_tag="ACL8CL_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HGS1329614.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            33058..33681
                     /gene="rplD"
                     /locus_tag="ACL8CL_000301"
     CDS             33058..33681
                     /gene="rplD"
                     /locus_tag="ACL8CL_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HGS1329615.1"
                     /translation="MANVTLFDQTGKEAGQVVLNDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            33681..33977
                     /locus_tag="ACL8CL_000302"
     CDS             33681..33977
                     /locus_tag="ACL8CL_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HGS1329616.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            33995..34828
                     /gene="rplB"
                     /locus_tag="ACL8CL_000303"
     CDS             33995..34828
                     /gene="rplB"
                     /locus_tag="ACL8CL_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HGS1329617.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            34932..35213
                     /gene="rpsS"
                     /locus_tag="ACL8CL_000304"
     CDS             34932..35213
                     /gene="rpsS"
                     /locus_tag="ACL8CL_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HGS1329618.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            35225..35569
                     /gene="rplV"
                     /locus_tag="ACL8CL_000305"
     CDS             35225..35569
                     /gene="rplV"
                     /locus_tag="ACL8CL_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HGS1329619.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            35582..36235
                     /gene="rpsC"
                     /locus_tag="ACL8CL_000306"
     CDS             35582..36235
                     /gene="rpsC"
                     /locus_tag="ACL8CL_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HGS1329620.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            36239..36652
                     /gene="rplP"
                     /locus_tag="ACL8CL_000307"
     CDS             36239..36652
                     /gene="rplP"
                     /locus_tag="ACL8CL_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HGS1329621.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            36662..36868
                     /gene="rpmC"
                     /locus_tag="ACL8CL_000308"
     CDS             36662..36868
                     /gene="rpmC"
                     /locus_tag="ACL8CL_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HGS1329622.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            36893..37153
                     /gene="rpsQ"
                     /locus_tag="ACL8CL_000309"
     CDS             36893..37153
                     /gene="rpsQ"
                     /locus_tag="ACL8CL_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HGS1329623.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            37179..37547
                     /gene="rplN"
                     /locus_tag="ACL8CL_000310"
     CDS             37179..37547
                     /gene="rplN"
                     /locus_tag="ACL8CL_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HGS1329624.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            37625..37930
                     /gene="rplX"
                     /locus_tag="ACL8CL_000311"
     CDS             37625..37930
                     /gene="rplX"
                     /locus_tag="ACL8CL_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HGS1329625.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            37954..38496
                     /gene="rplE"
                     /locus_tag="ACL8CL_000312"
     CDS             37954..38496
                     /gene="rplE"
                     /locus_tag="ACL8CL_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HGS1329626.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            38514..38783
                     /gene="rpsN"
                     /locus_tag="ACL8CL_000313"
     CDS             38514..38783
                     /gene="rpsN"
                     /locus_tag="ACL8CL_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HGS1329627.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            39068..39466
                     /gene="rpsH"
                     /locus_tag="ACL8CL_000314"
     CDS             39068..39466
                     /gene="rpsH"
                     /locus_tag="ACL8CL_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HGS1329628.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            39658..40194
                     /gene="rplF"
                     /locus_tag="ACL8CL_000315"
     CDS             39658..40194
                     /gene="rplF"
                     /locus_tag="ACL8CL_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HGS1329629.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEVTLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            40278..40634
                     /gene="rplR"
                     /locus_tag="ACL8CL_000316"
     CDS             40278..40634
                     /gene="rplR"
                     /locus_tag="ACL8CL_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HGS1329630.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            40652..41146
                     /gene="rpsE"
                     /locus_tag="ACL8CL_000317"
     CDS             40652..41146
                     /gene="rpsE"
                     /locus_tag="ACL8CL_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HGS1329631.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            41160..41342
                     /gene="rpmD"
                     /locus_tag="ACL8CL_000318"
     CDS             41160..41342
                     /gene="rpmD"
                     /locus_tag="ACL8CL_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HGS1329632.1"
                     /translation="MAQIKITLTKSPIGRIPSQRKTVVALGLGKLNSSVIKEDNAAIR
                     GMITAVSHLVTVEEVN"
     gene            41487..41927
                     /gene="rplO"
                     /locus_tag="ACL8CL_000319"
     CDS             41487..41927
                     /gene="rplO"
                     /locus_tag="ACL8CL_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HGS1329633.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            41940..43250
                     /gene="secY"
                     /locus_tag="ACL8CL_000320"
     CDS             41940..43250
                     /gene="secY"
                     /locus_tag="ACL8CL_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HGS1329634.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            43401..44039
                     /locus_tag="ACL8CL_000321"
     CDS             43401..44039
                     /locus_tag="ACL8CL_000321"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HGS1329635.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGVINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQNINDVFSDIEKVLTNLK"
     gene            44156..44374
                     /gene="infA"
                     /locus_tag="ACL8CL_000322"
     CDS             44156..44374
                     /gene="infA"
                     /locus_tag="ACL8CL_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HGS1329636.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            44399..44515
                     /gene="rpmJ"
                     /locus_tag="ACL8CL_000323"
     CDS             44399..44515
                     /gene="rpmJ"
                     /locus_tag="ACL8CL_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HGS1329637.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            44533..44898
                     /gene="rpsM"
                     /locus_tag="ACL8CL_000324"
     CDS             44533..44898
                     /gene="rpsM"
                     /locus_tag="ACL8CL_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HGS1329638.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            44916..45299
                     /gene="rpsK"
                     /locus_tag="ACL8CL_000325"
     CDS             44916..45299
                     /gene="rpsK"
                     /locus_tag="ACL8CL_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HGS1329639.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            45342..46277
                     /locus_tag="ACL8CL_000326"
     CDS             45342..46277
                     /locus_tag="ACL8CL_000326"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HGS1329640.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            46289..46675
                     /gene="rplQ"
                     /locus_tag="ACL8CL_000327"
     CDS             46289..46675
                     /gene="rplQ"
                     /locus_tag="ACL8CL_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HGS1329641.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            46940..47206
                     /locus_tag="ACL8CL_000328"
     CDS             46940..47206
                     /locus_tag="ACL8CL_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HGS1329642.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            47216..48553
                     /locus_tag="ACL8CL_000329"
     CDS             47216..48553
                     /locus_tag="ACL8CL_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HGS1329643.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMEGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMKVNGASSVDFISRGGQIPAPIHSFKN"
     gene            48797..49489
                     /locus_tag="ACL8CL_000330"
     CDS             48797..49489
                     /locus_tag="ACL8CL_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS1329644.1"
                     /translation="MVKVRLYLVRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEIIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
     gene            complement(49592..51244)
                     /locus_tag="ACL8CL_000331"
                     /pseudo
     CDS             complement(49592..51244)
                     /locus_tag="ACL8CL_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(51234..52324)
                     /locus_tag="ACL8CL_000332"
                     /pseudo
     CDS             complement(51234..52324)
                     /locus_tag="ACL8CL_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289361.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(52337..53458)
                     /locus_tag="ACL8CL_000333"
                     /pseudo
     CDS             complement(52337..53458)
                     /locus_tag="ACL8CL_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003085230.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            complement(54045..54821)
                     /locus_tag="ACL8CL_000334"
     CDS             complement(54045..54821)
                     /locus_tag="ACL8CL_000334"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HGS1329645.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVDFIYTDLKHYNSIKHKKVTGVFN
                     QMIIKNIHYAFSQNKTIVLRIPVIPNFNNSLEDAEKFATLFNSLNIDQVQLLPFHQFG
                     ENKYRLLNRKYEMDGINALHPEDLIDYQKVFLNHHINCYF"
     gene            54943..55689
                     /locus_tag="ACL8CL_000335"
     CDS             54943..55689
                     /locus_tag="ACL8CL_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1329646.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            55702..56682
                     /locus_tag="ACL8CL_000336"
     CDS             55702..56682
                     /locus_tag="ACL8CL_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HGS1329647.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
     gene            56877..57197
                     /locus_tag="ACL8CL_000337"
     CDS             56877..57197
                     /locus_tag="ACL8CL_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1329648.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
     gene            57243..57551
                     /locus_tag="ACL8CL_000338"
     CDS             57243..57551
                     /locus_tag="ACL8CL_000338"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1329649.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            57568..58866
                     /locus_tag="ACL8CL_000339"
     CDS             57568..58866
                     /locus_tag="ACL8CL_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS1329650.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            59017..61455
                     /locus_tag="ACL8CL_000340"
     CDS             59017..61455
                     /locus_tag="ACL8CL_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HGS1329651.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            61474..62142
                     /locus_tag="ACL8CL_000341"
     CDS             61474..62142
                     /locus_tag="ACL8CL_000341"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HGS1329652.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            62160..63248
                     /locus_tag="ACL8CL_000342"
     CDS             62160..63248
                     /locus_tag="ACL8CL_000342"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HGS1329653.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNCPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            63703..66204
                     /gene="leuS"
                     /locus_tag="ACL8CL_000343"
     CDS             63703..66204
                     /gene="leuS"
                     /locus_tag="ACL8CL_000343"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HGS1329654.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEEAAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLDVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
     gene            66343..67185
                     /locus_tag="ACL8CL_000344"
     CDS             66343..67185
                     /locus_tag="ACL8CL_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329655.1"
                     /translation="MNRLVNDSRKCEILLFKISIIVVSVIVLVMSIVGAYYFKNCLWD
                     KESLNLTLTLDTLFAVFITFFSFSNDKSSNIFGISPNEIIRTQSEVYQFLFTKSFATL
                     LIVTNLLSIIHYFGAKNLIVSFNETYPRIQSSVGIMWLPLFGILIIYFIWLLIEGLKY
                     IRKINYIIQNREIIYFYSQVKVVDKLTDSESKKYGEYIDLYESCRQNNLSVLLNFLQN
                     SDDKRGVIASNSVRIMFESLDKADQIAALNICRNYVARENVLNKEDAEKLLNEIDKKL
                     EISR"
     gene            67241..67936
                     /locus_tag="ACL8CL_000345"
     CDS             67241..67936
                     /locus_tag="ACL8CL_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS1329656.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            67948..68364
                     /locus_tag="ACL8CL_000346"
     CDS             67948..68364
                     /locus_tag="ACL8CL_000346"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1329657.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
BASE COUNT        21098 a        12163 c        14586 g        20570 t
ORIGIN      
        1 tgatttactt ttaagctact tagttgaaaa cgaaaaattg atcagaattc gttagatttc
       61 agtaaatctg gataattatt gtatcgagta tgaatatttt gaaaaaaaga agaattttat
      121 gtatttggag aagaaaatgc aaatattaat ttctgaagta aatgtaacta gttttggaat
      181 tacaagagaa attttaaaga aaatggattt taatttctca aaaatgggta gcgaagaagc
      241 taaattttta atttttccag agttacctag aggaggtgag ctaagtttaa ctgctcgtca
      301 aagttatgat agccagttaa ggtattcgat gttacctaga gttgaggaca tcatgaagaa
      361 aattgtaaat caacttagag aacatttaaa aattttagac attgagctaa gtgatttaag
      421 ttatttatca gagggtgatc aattcatatt aaaggaacta tcaagtcatg taaaaataaa
      481 tattaaaatt actccaataa aaaaaatacg ttatgtatat gaagatatct ttctcaatca
      541 atcagcagag gaattacatg aattaggatt aaaatattta tcagcaggtg atttatgggc
      601 ttctaaaaaa atatttactt acttagtaaa taaatatgat aatagacgag atgttttcaa
      661 attagctaca acttataata tgttattgga aacagaaaaa gcagaatact actacaacct
      721 atacaaacat gatggaaaag ttgaccatat tgtggcagca aactatatgc ttgcaatgct
      781 ctatgcacgc catcatccta aataccttca agatgatcaa aaagctattg tattattgaa
      841 tgaagcgtat gatttaatta aagataaagt gacattagag agtattttta atcgaaatgg
      901 gtatgcccta ctattattta aaatgggcaa agtagaggaa acaatcatat tattaaaacg
      961 ttgtttaaat caaataaaac aattagataa ctctagttct tcggttgttc ttcatgaaag
     1021 tgtactttgg tttaatttat atcagtgcta tgtcactata ggtgacaata ctaattctcg
     1081 gaaaactttt tatattttga aaaatatgga tccgcttttt attgagtact atctagaata
     1141 ttctagaaat cttttagaga cagatgtcga aaattttcta gaatttatag agagtatgcc
     1201 acatcattct aataattttg ctgaaaaata ttcgctccta ggctactatt ttagaggtac
     1261 tgcgaaatca ttagaatatt ttagtaaagc ttttgaattg tcaaatagaa cgttttcttt
     1321 ttggtacgat ataacttatg aactaacgga gtttaatctt tacaatgaag catacaataa
     1381 tttaccttta ttgaataata aattgtatga gctgaatgat gaagacttac taaattatat
     1441 tttaatttcg gcagaaattt tttataattt agacaggttg gatgaattta aacgaactat
     1501 agaaattggg tatagaattt ttcctgattc agaaagtatt aaagaaatta tagagaatgc
     1561 cgaatgaaaa gatataaaac tctatttata aagtatcctg attctttcca gtttttagtt
     1621 tttaatattc tgttttattt atcaatgtca ctttctagga gtgttcattc aatttggttt
     1681 gatttgaatg gaagtttact gaattatagt atttcatatt cagcaatggc tttttcagga
     1741 atgtttgctg gaattctagg cagaagaatt aattatttta atttaagaaa tctgataaga
     1801 ataggagtaa tactatattc tatcggttta ttattgagat tatttcctaa tgattggttg
     1861 atatcattaa tctctggtct tatctcaggt attggagcgg caacaataat tcttttattg
     1921 cgaacatggg ctgtcggaat aggaaaagaa aaagacagag tagttacgat ttccttaaaa
     1981 agattttcca gcagtttagg aacttctatt ggagggatat tgtcaggcat tttgctatat
     2041 tgcttaatat atttttttca tagtatggcg tatcctattg ttttagcttc ggcaagtatg
     2101 ctatgtttag taactttctt ttgtattcct aaaattgaca gttttgttga taagagtgaa
     2161 agagcagaaa gaatcttaga atctagtaat tacatgaaaa agagaataat cttttttggt
     2221 atcttctcag gtttaattgc tagtatattt tctccttatc tacccgttat tttaagacgt
     2281 caagaaatat cagaagtttt tattgggcct gttctaacta ttttaagtct attaacaatt
     2341 tatttctctt ccatattttc aagtagaaaa gtagataatc ataaacaagg atattatatt
     2401 ttatccgagt tgttgatttc gatacttact ttattattta gttttcattt aaaatattat
     2461 ttagtaataa cgatattgat tattagagta ttttttgtaa acctatcatc aattacacaa
     2521 gagttaatgg aattatcaat ttatccaaag gaaaaaattt catatttcta tggcatttct
     2581 caaacttcct attttatagg tgatactctg ggaggattta ttggaggttg gctatttaac
     2641 caagatatga aatacagtat ttatctgttt tctgtgttat tattattcaa cgctgtgtta
     2701 tttaacagat tttacttacg gaataataaa aattcttaag ggacctctaa aaaaaacaga
     2761 agattaaaat acagtaaaaa aatcaactaa aatagtccca aagtaaagaa atacttgaac
     2821 ctattttagc tggttttctt ttttttcacc tctaattgag gattattcta ttttagggta
     2881 tactccccca tgttttcaac cctaatcgtt ggatttgttc gaaacgacag atattataca
     2941 acaaatttgt caaaatcaca ttcgtttttg tacgcgctaa tccaattcct ctaaaatgac
     3001 ttcctttcat cgtattttta ataacaccaa aaacatgttc cacacgacag cggatacgag
     3061 aaatagactg attgatacgc ttatccaaat cagtcaaggt tttatttcga aaagcactat
     3121 catcaaagat cggctcctta ctctcacaca actcttccaa gacgcgagaa tcgtgaatat
     3181 tagcagaagt aatctcaaat ttcttgatta gttttgattt ttggtcgata ctaatactca
     3241 atgaaaatca aagagcaaac taggaagcta gacgcaagtt gctcaaaaca ctgttttgag
     3301 gttgcagata gagctgacgt ggttttaatt tgattttcga agagtataat atgatttgta
     3361 taaccaaagt aagttggttc tccctagtcc atcctgtatc cctatctttt tgagaaggct
     3421 tcgctgtatt ccaatctgtc ggaatctttc cttttttgat ttgttgattc tcttctcgtg
     3481 tattgcgttg tttaggggat tctacaaatg gggcatctac tatctgtccc tgatgagcta
     3541 ataagccttt ttctgctagt gttagataaa attactcaga taattcttgt tctttctctg
     3601 atttggtcaa gcgttctcta tacaaccaga tggtttttgc atcatgtact ttacattcat
     3661 cacaacctgt ggaatgacga aatgacagac gatgaaagag ttgatattcc atcgcatcat
     3721 ctgataaatt atggagatgt tggatcaaga gcaccttaaa tatcatgaaa aaatcatgcg
     3781 gagggagtcc gccttttgat ggtttctaaa aatctgctca agaataggtc taatttgctc
     3841 ccaatccatt gccttatcta agccttccaa tggattgccc attgaactca attttgatag
     3901 tgtttgttca tcatctataa aaaattccat cgcctaacct agcttacctc tatctctagt
     3961 atctcatgtt ttacgatttt tttgggtttt tagaggtgcc cttaaattcg ttcattaatg
     4021 ttacgagact ggccgaaaat tagtctctaa ataaaaccac acagtgattt cagtcttgaa
     4081 tccaatcaaa ctgaaagaag cactatgtgg tttttgaata gtgggctaga ttcttgatca
     4141 atttggtgag attcatggta atcgcccaat aacgaggtat attgaaaaat ctacagacta
     4201 gagaactcac tgatagttcc taatctggag atttcttatt tgcacttttc ttgtacaact
     4261 ttagtccatg gtaaataagc ctctaaaaca tctttattta cgagagtctc ctcgcctagc
     4321 tttctagttt gttctttaat ttttattgag tataccacta ttttactccc tctggcaagg
     4381 gactttgtct atgtggaggg attgggctcc tatgtggtgg agcttttctg ttctttctga
     4441 aatatggtat aatagcacta atcaatttct aggaaaatag atacagaaag gggctgaaag
     4501 atgtctcata ttattgaatt gccagagatg ctggcaaacc aaatcgcggc tggagaggtc
     4561 attgaacgtc ctgccagtgt ggtcaaagag ttggtagaaa atgccattga cgcgggctct
     4621 agtcagatta tcattgagat tgaggaagct ggtctcaaga aggttcaaat cacagataac
     4681 ggttatggaa tttcccacga tgaggtggag ttggccctgc gtcgccatgc gaccagtaag
     4741 ataaaaaatc aagcagatct ctttcggatt cggacgcttg gttttcgtgg tgaagccttg
     4801 ccttctattg cgtctgttag tgtcttgact ctgttaacgg cggtggatgg tgctagtcat
     4861 ggaaccaagt tagtcgcgcg tgggggtgaa gttgaggaag tcatcccagc gactagtcct
     4921 gtgggaacca aggtttgtgt ggaggatctc tttttcaaca cgcctgcccg tctcaagtat
     4981 atgaagagcc agcaagcgga gttgtctcat atcattgata ttgtcaaccg tctgggcttg
     5041 gcccatcctg agatttcttt cagcttgatt agtgatggta aggaaatgac gcggacagct
     5101 gggactggtc aactgcgcca agctattgca gggatttacg gtttggtcag tgccaagaag
     5161 atgattgaaa ttgaaaattc tgacctagat ttcgaaattt caggttttgt gtccttgcct
     5221 gagttgactc gggctaaccg caattatatc agccttttca tcaatggtcg ttatatcaag
     5281 aatttcctac tcaatcgtgc tattttagat ggttacggaa gtaagctcat ggtaggccgt
     5341 tttccactgg cagtcattca catccatatc gacccctatc tagcggatgt caatgtgcat
     5401 ccaaccaagc aagaggtgcg aatttctaag gaaaaagaac tgatgacgct ggtctcagaa
     5461 gctattgcca acagtctcaa ggagcaaacc ttgataccag atgccttgga aaatcttgcc
     5521 aaatcgaccg tgcgcaatcg tgagaaggtg gagcagacca ttctcccact caaagaaaat
     5581 acgctctact atgagaaaac tgagccgtca agacctagtc aaactgaagt agctgattat
     5641 caggtagaat tgactgatga agggcaggat ttgaccctgt ttgccaagga aaccttggac
     5701 cgattgacca agccagcaaa actgcatttt gcagagagaa agcctgctaa ctacgaccag
     5761 ctagaccatc cagagttaga tcttgctagc atcgataagg cttatgacaa actggagcga
     5821 gaagaagcat ccagcttccc agagttggag tttttcggac aaatgcacgg gacttatctc
     5881 tttgcccaag ggcgagatgg actttacatc atagatcagc acgctgctca ggaacgggtc
     5941 aagtacgagg agtaccgtga aagcattggc aatgttgacc aaagccagca gcaactccta
     6001 gtgccctata tctttgaatt tcctgcggat gatgccctgc gtctcaagga aagaatgcct
     6061 ctcttagagg aagtgggcgt ctttctagca gagtacggag aaaatcaatt tattctacgt
     6121 gaacatccta tttggatggc agaagaagag attgaatcag gcatctatga gatgtgcgac
     6181 atgctccttt tgaccaagga agtttctatc aagaaatacc gagcagagct ggctatcatg
     6241 atgtcttgca agcgatctat caaggccaat catcgtattg atgatcattc agctagacaa
     6301 ctcctctatc agctttctca atgtgacaat ccctataact gtcctcacgg acgtcctgtt
     6361 ttggtgcatt ttaccaagtc ggatatggaa aagatgttcc gacgtattca ggaaaatcac
     6421 accagtctcc gtgagttggg gaaatattaa aagtataaaa aagtctggga aaaattttca
     6481 aaatcaaaaa aacgcataaa atcaggtgtt caaaaacctt gattttatgc gttttatcat
     6541 ggaaatagtt acttcatttt ttcctaattc ttttcgaaac tctttttaaa cgacgtcagt
     6601 tttatcagta atctcaaaac agtgttttga gctaattttg ccagttttgt ctgtaacatc
     6661 gaagttgtgt ttgaccactc tgcgactggt ttcctagttt gctctatgat tttcacagag
     6721 tattaaattg cgattttgcc aagtttcttt attcgtctaa aagtagagtc tgttctatgc
     6781 gtctaatgta cgaattaggt tgaccatttc aatagctcct tgtgcacact cagaaccctt
     6841 atttcctgct ttagtaccag ctcgttctat ggcttgttca attgtatctg tcgttagcac
     6901 accaaacata acaggaattt cgctatttaa actgatttgg gcgattccct tagatacctc
     6961 gctacataca taatcataat gacttgtatt tcctctaatg acagctccca agcagataat
     7021 tgcatcatat tttttacttt ttgccatttt tgatgcaatc agtggtattt caaaagctcc
     7081 tggaacccag gctacctcga tatctttctc gtttacattc tctcttttga gattatctag
     7141 tgctccagat aataattttg aagttataaa ttcattaaat ctcgctacaa caatacctat
     7201 tttaatattg tttgctacta aattaccttc ataagtgttc atttattttt cctccatatt
     7261 taaaatgtga cccattcgat ttttctttgt ttctaaataa aaactatcgt aaggattggc
     7321 ttctatttcg attgatattc tactggaaat ggtaattcca tatttttcta actgttcaac
     7381 cttgtcagga ttatttgtca gtaaatgaag tgactgaagt cccagatctt taagcatttg
     7441 tgctccaata tgatattctc ttaaatcacc ttcaaagcct aatgcaagat tggcatcaag
     7501 cgtatccatg ccttgatctt gtaaatgata ggcttttaat ttattgataa gtccaattcc
     7561 tcgtccctcc tgtcgcaagt aaagtaagac acccgaacca ttctcaacaa tcattttcat
     7621 agctttatcg aattgctgtc cacaatcgca acgtaaagag cctaaaacat ctcctgttaa
     7681 acattcggag tggacccgac ataatacatt ggcttcatcc tctatgtttc ccataataag
     7741 agcaagatga tgttccccat ttagtttatc tatatagcta attgctttga aattaccgta
     7801 tctagtaggc atattgacag ttgaaactcg ttctaccagc tgatcatata cttttctata
     7861 ttcttgtaat tctttgatgg taattagtgg aatgttgtgt ttcttcgaaa actgaattaa
     7921 atcatctgtt ctcatcattt tgccatcatg attcattatt tcacaacata ggccacactc
     7981 ttttagtcca gctaatttta ataaatcaac agttgcttct gtgtgtccat ttctttctag
     8041 aacaccacct ttttttgcaa ttaaaggaaa catgtgtcct ggcctgcgaa aatcagaggg
     8101 tgttatatct tcagctacac acatacgtgc ggtcagtcct ctttcctcgg cagaaatacc
     8161 tgtggtcgtt tctttataat caattgaaac tgtaaaagca gtcttatgat tatctgtatt
     8221 gttttcaacc ataggtgaaa gcattaattg attagctaaa ctttcgctca taggcataca
     8281 aattaatcct ttggcataag tagccataaa attaacattt tctgttgtag ctgcttgtgc
     8341 agaacaaatt aagtctcctt cattttctct atccttgtcg tctataacaa gaacaagtcg
     8401 tcccttctgc aatgcttcta atgcttcttg tatttttcga tattccattg actgattatc
     8461 ctttctgcta aaatccattt tgatataata gttccttaga tatttctgat tttggagagt
     8521 tatccatcag tttttgcaca tatttaccta agatatcatt ttcaagattt actgtactcc
     8581 cgacttgttt actcttaaga atggtttgtt ccaaggtatg agggataaca gatactgaaa
     8641 agtttacttt ggagacttta gcgacagtca gactaatgcc gtcaattgta atagatcctt
     8701 tttcaactat taaatctaaa atttcttttt gtgtgttgat ttgataccat acagcattat
     8761 catctttttt tattgacgag atttttcctg taccatcaat gtgtcctgta acgacgtgac
     8821 ccccaagtcg accgttgaca gataaggctc tttctagatt cacctcactt ccatgtttta
     8881 atagagtaag agctgttcga ctccatgttt cattcattac atcaactgta aaggattgat
     8941 ggttgaaatg agtaactgta agacagatac catttactgc tatactatcg cctaaatgga
     9001 tatccgttaa tatttttgag gctttaattg atagtttaca attacgagag tctttctgta
     9061 ttctttcaac ttttccgatt tcttcaatta ttcctgtgaa catggataaa tcacttcact
     9121 ttctatgaga tagtcatttc ctatttgaga aaaagcataa ggtttcaatc taatagcgtc
     9181 atttggcaaa gaaatgcctt cacctccgac aggaaacttg gcactgcctc caaaaatttt
     9241 tggtgcaata tatattttca gctcatcaac aatttgttgt tccaaagcac tccaattcat
     9301 tagactgccc ccttctagaa ctaggctatc aatctgcatg tttcctagat gttgcattaa
     9361 actcgataag tctatatgat tgcctttttt ctttatggaa agtatttcac agccatgatt
     9421 ttgatatagc ttcattttat ttttgtcttc agaggaagtg gcaatgtaag ttttaatatc
     9481 atttgctgtt tttacgattt tagaggtaag aggagttcgt aaatgtgtat cgcatatgat
     9541 acggatagga tttttccctt cctccaatct acatgtcagc aaaggatcgt cttgaataac
     9601 agtattgact cccaccataa ttgcactaac atagtgtcgt aactgatgca catgctttct
     9661 tgcttcttct tcagtaatcc atttggattg atttgtttta gtggctattt ttccatccat
     9721 tgacattgca tatttcataa aaacataggg tacatgctgg gtaatatact ttctaaaact
     9781 ttttattaag ttaagacact cattttctaa aattccaaca gtaacttgaa gattattttc
     9841 ctcaagtatc tttactcctt ttccagatac aataggatta cagtctaggc ttccaatgac
     9901 tactcttgta ataccactat cgattatagc atctatacag ggaggtgttt tcccgaagtg
     9961 acaacagggt tcaagtgtta cataaagcgt cgctccgaca ggggattctc tacagttttt
    10021 aagagcattt ctctcagcat gtgggccacc aaaaaactca tgataacctt gtccgatgat
    10081 gtgattatct tttacaataa ctgcgccgac cataggattg ggattgacgt aaccagcccc
    10141 tttttgtgcc agttttattg ctaatttcat atattttgaa tcgctcatct cactacctcc
    10201 aaaaaaaata taccttgaat aggggactac tcaaggcata caaaagaaaa cttatgcgat
    10261 taacaaaaat actctgaaat gacaagtaat catttcagag cacgcaaaaa gcacaaatat
    10321 acttttatct tctttcatcc agactatact gtcggctttg gaatttcacc aaatcatgcc
    10381 tttcggctcg tgggctatac caccggtagg gaattacacc ctgccctgaa gatagttatt
    10441 caattacaga tgattatagt acttaatttt gaatatgtca acagataaat accgattgtt
    10501 tttgatatac tgtatttgtg ataatcgatt ctcgctcctc ggataaagaa aatatgatat
    10561 actagataaa cgaaataaga gagaaggaat actatgtacg catatttaaa aggaatcatt
    10621 accaaaatta ctgccaaata cattgttctt gaaaccaatg gtattggtta tatcctgcat
    10681 gtggccaatc cttatgccta ttcaggtcag gttaatcagg aggctcagat ttatgtgcat
    10741 caggttgtgc gtgaggacgc ccatttgctt tatggatttc gctcagagga tgagaaaaag
    10801 ctctttctta gtctgatttc ggtctctggg attggtcctg tatcagctct tgctattatc
    10861 gctgctgatg acaatgctgg cttggttcaa gccattgaaa ccaagaacat cacctacttg
    10921 accaagttcc ctaaaattgg caagaaaaca gcccagcaga tggtgctgga cttggaaggc
    10981 aaggtagtag ttgcaggaga tggccttcct gccaaggtcg cagtgcaagc aagtgctgaa
    11041 aaccaagaat tggaagaagc tatggaagcc atgttggctc tgggctacaa ggcaacagag
    11101 ctcaagaaaa tcaagaaatt ctttgaagga acgacagata cagctgagaa ctatatcaag
    11161 tcggccctta aaatgttggt caaataggag cagagaatga caaaacgttg ttcgtgggtc
    11221 aagatgacca acccgctcta catcgcctat catgatgagg agtggggtca gcccctccat
    11281 gatgaccaag cattgtttga gttgttgtgt atggaaacct atcaggcagg cctgtcttgg
    11341 gaaacggtac tcaacaaacg ccaagctttc cgagaagcct ttcatagcta tcaaattcac
    11401 tcagtcgcag agatgactga gactgaattg gaagccatgc tggagaatcc agctatcatt
    11461 cgaaatagag ccaagctttt tgctacacgc gctaacgccc aagcctttct acagttacag
    11521 gcagagtacg gctcttttga tgcctatctt tggtcttttg ttgaggggaa aactgtcgtt
    11581 aacgatgttc ctgattatcg ccaagcccca gctaaaacac ccttatctga gaaattagcc
    11641 aaagatctca aaaaacgagg cttcaagttc acaggcccag tcgccgtatt gtcttttcta
    11701 caggctgcag ggctagttga tgaccacgag aatgattgtg agtggagagg tcttaaatga
    11761 tgtctaacaa aaataaggga attctgattt ttgcgattct ctatacagtc ctctttatgt
    11821 ttgatggcgt taaattgctg gcttctttaa tgccatctgc cattgcaaat tatcttgttt
    11881 atgtagtttt agctctatat ggctccttct tgttcaagga tagattgatc caacaatgga
    11941 aggagattag aaagactaaa agaaaattct tctttggcgt cttaacagga tggctctttc
    12001 tcattctgat gactgttgtc tttgaatttg tatcagagat gttgaagcag tttgtgggac
    12061 tagatggaca aggtctaaat cagtctaata ttcaaagtac ctttcaagaa caaccactac
    12121 tgatagctgt ttttgcttgt gtcattggac ctctggtaga agaattattt ttccgtcagg
    12181 tcttattgca ttacttgcag gaacggttgc caggtttact aagcattatt ctggtaggac
    12241 ttgtttttgc tttgactcat atgcacagtt tggctctatc agagtggatt ggtgcagttg
    12301 gttacttagg tggaggcctt gccttttcta ttatttatgt gaaagaaaaa gagaatatct
    12361 actatcccct acttgttcac atgttaagca acagcctctc cttaatcatt ttagctatca
    12421 gtatagtaaa atgaaataag aacaggacaa atcgatcagg acagtcaaat cgatttctaa
    12481 caatgtttta gaagcagagg tgtactattc tagtttcaat ttactgtaat atgtgatgaa
    12541 aatgccagta atgataccga gaaaaaagct gagaaacttt tcccagcttt atttgttata
    12601 gtcaaagcga atgacttgtt cctgtgcatc tacatgagca tggaccccaa agggtacaat
    12661 tgctcttgga gttgcgtggc cgacattcag attatagaca atcgggatat tgctgtcaat
    12721 gatatccaat agtgcctctt tatagtcgtc atggaaagtt tcatccatag gttttccgac
    12781 caagagtcca ttgatgaccg cgaatatgcc agtgtccttt aaagttagca acatcttttt
    12841 gaagtcttct ggcttaggct tttcttcgct tgtttcgagc aagaggattt tcccttccca
    12901 gtctgacaag tcagggaaaa gtttgtattt ttggcagagc tccgtgctat ctgcgtatcg
    12961 agagttgtca aagatatcgt agagggattc gaggcaacca ccgaggattt tcccctcgaa
    13021 ttgggccctt ccttgcaaca agtcaaaacc tgtatttgta tgactgacac gaggtgttcc
    13081 cagggccgtg ggactaaaat cagttcgttc ctcataccaa acgtcactag ggcggatttc
    13141 tgaaattctt cccgtctcaa tcaattcttt aaagtagtga aggctatagg ctagcatttc
    13201 tttgtctaat tcacaaatgt ctgctaaaaa ggattgacca taaaaagtct tgattcctaa
    13261 tttatgcaac atgaggtggt tcatggttgt atccgagaag ccaagaaaaa ttttttgttt
    13321 gataaccttt tggagttggt cattttcaaa aagataaggt agcaagcgat aggtatcgtc
    13381 tccaccgatg gcacatagga tcatgtcgat gctatcatca gaaaaggcat gaatcaaatc
    13441 ctctgcacga gcttcaggat ggtccttgat aaagtctaat ccttttaacg aatggggcaa
    13501 aaagatggga ttgagtccca gatccttgag acgttggata cccaagtcca cttcgtgttt
    13561 gacaaaatcc tctccgataa tgccactaga taaactaaca ataccaatag tagaaaccat
    13621 atctcatcct cctagaaata gattgagcct attttatcac aaaatataag agaaagctat
    13681 gtgggatttc agaagaatac ttttaatact cttcgaaaat ctcttcaaat cacgtcagtt
    13741 ttatttgcaa cctcaaagct gtgctttgag caacctgcag ctagcttcct agtttgctct
    13801 ttgattttca ttgagtataa ctcaaaaaag taagtgaaaa agcaaccgca cctgcagttg
    13861 cttttatcgt tttcttattc ttgatagcgt gttggtcctg cacttgcact acggatattc
    13921 aagcgtttct tgagatagaa gcgaagggct aggagggctg ctccaataat agctagtggc
    13981 aatggatcca gtactgggtt aaggctagct ggtaggaagc ttgttgcaaa gaagacaagc
    14041 aaccaaagga acatagaagc taggataact agtacagatt tccagaaagg tggacgttga
    14101 ctgcgatcca tatctggtcc atagtattgg taaacaaagt agtacatcaa gtagaaggca
    14161 aatccaccaa ccagtccaac taatagaaga gtaatcaatc catagccgaa agcttgatct
    14221 gccgcaaaga aggttgtgag ggcgctgaca agggcaaaga ggctagtgat gaaaagagct
    14281 gagtccataa tcatcagttt tgggtcatca ttttcttttg gatgctcttt ttcgtactgc
    14341 tctttgacag tgaagctatg agcccaatga gttggtgcgc cgtataggga acgggcagtt
    14401 gtacctttag attgctcctc aaggatttgg ggaatgactt cctcaaaaat agtctggatt
    14461 tcagcgtctg ttttcccatc tttgatgaat tgttgggtag caatgtggac aaactcttgg
    14521 tttttcttag ttaatttttg tagatcaatt tgagacatag gaactcctct tagaaccatt
    14581 ttttatggat gagatagaga gtgagcgaga cactcatagc aaaggcgata aagacgatta
    14641 accagaaggc atttggctct ccgtttaggg gaatttcatt atccttaaag ttcatcccgt
    14701 aggcagaaaa gaccatggtt gggatggaca tgacgatggt cacaagggcc aaggttttca
    14761 tgatgttgtt ctggttgtta gaaatgatag aggcaaaggt ctctgtcata gaatgcaaga
    14821 cgtttccata aatatctgcc atctcgatgg cctgttgggt ttcaatcagg gtgtcttcaa
    14881 gcaggtcttc gtcctcaagg tatttcttga tattgctggt tgaactggtc aatttcttaa
    14941 tcacgcgctc atttgttttg agggaggcct tgaaatagac gatagttttt tccaattcca
    15001 tgagctcaat caattcttca ttacgagttg attgatgcag ttgactttcg atttgttcac
    15061 tcttgcggtc gattgaacga agggctgtta ggtaaagctc tgcattgcga taaagaattt
    15121 gaaagataaa acgtgaacgc atgaaggtat agaaattacg caatcgacgg ttgataaaga
    15181 catcaaggac aggtagtggt tccaaacacg tagtgataat ggtttcctca gtgatgataa
    15241 taccaagcgg gatggttacg tagtaggtgc ggttatttct ttcctccgtg accggcacgt
    15301 ctacgataat cagggtatac tcgtcttcaa tggtaatacg agacatttct tccgcatcga
    15361 gcggtgctcg aaggtcggca atatcaatat cgaaggtgtt ggcgatttcg agtgattcat
    15421 tttgagtcgg attgacgaga ttgatccaag tacccggttc aagcgtatcg atctctttaa
    15481 attcagttgt tgtagagaga aaaacttgtt tcatatccct tatcctttct catttttcag
    15541 attttttcac actgtactat tatactacaa aatcggcctt ttgggtaata gaatctcact
    15601 ttttttggta taatggtaag caataatgga ctagaaagaa caaagatgca agataaaatt
    15661 gtcattcatg gggcgcgtgc ccataattta aaaaatattg atgtggagat tccgcgagac
    15721 aagttggttg tcgtgactgg cttgtcaggt tcagggaagt ccagtctggc ttttgatact
    15781 ctctatgcgg agggacaacg tcgctatgta gagagtttgt ctgcctacgc tcgtcagttc
    15841 ttgggaaata tggaaaaacc agatgtagat gccattgatg gtctcagccc agctatttcc
    15901 atcgaccaga aaacgactag taaaaatcct cgctcgacgg tgggaacgac gactgaaatc
    15961 aatgactatc tgcgtctcct ctacgcacgt gtggggacgc cttactgtat caacggacat
    16021 ggagctatca atgcttcttc tgtggagcaa atcgttgata aggttttgga gttacctgaa
    16081 cgccagcgct tgcagatctt ggctcctgtc atccgcaaga aaaaaggcca acataagagt
    16141 gttatcgaga aggttcagaa agacgggtat gttcgtgttc gtgtggatgg ggaagtctat
    16201 gatgtgaccg aagtgccaga gttgtctaag agcaagcaac acaatatcga tgtcgtggtt
    16261 gaccgtattg ttatcaagga gggcattcgt agccgtctct ttgattccat tgaagctgcc
    16321 cttcgtatcg cagaaggtta tgtcattatc gacaccatgg acgactcgga gttgctcttc
    16381 tctgagcatt atgcctgtcc agtttgtgga tttactgtcc cagagttaga acctcgtctc
    16441 ttctctttca atgctccctt tggctcttgt agtgagtgtg acggcttggg gattaagctg
    16501 gaggtggata ctgatttggt agtgccagat gccagcaaaa ccttacatga gggagcgctg
    16561 gcaccttgga atcctatctc atccaactac tatccaaaca tgttagagca ggccatgaaa
    16621 gtctttggag tggctatgga taagcctttt gaggacctgt cagaagaaga taagaacttg
    16681 attctctacg gctcagatgg taaggaattc catttccatt atgaaaatga atttggtggt
    16741 gtgcgcgata tcgacattcc gtttgaggga gttatcaata atatcaaacg tcgttaccat
    16801 gaaaccaata gcgattacac tcgcactcag atgcgtctct acatgaatga gctgacctgc
    16861 ggaacctgtc aaggctatcg tctcaatgac caggccttgt ctgtccgtgt gggcggccag
    16921 caagggccac atattggaga aatctcagac ctgtctatcg ctgaccactt ggacttggtg
    16981 agccagttaa ctttgtctga aaatgaagcc atcattgctc gtcccattct caaggaaatc
    17041 aaggatcgtt tgaccttcct taataatgtg ggtcttaact atctaaccct gtcacgttca
    17101 gcaggaaccc tttcaggtgg ggaaagtcag cgcattcgtt tggcaaccca gattggttcc
    17161 aacctatcag gtgtcctcta tatcctagac gagccgtcaa tcggtcttca ccagagggac
    17221 aatgaccgcc tgattgccag tctgaaaaag atgcgtgact tgggcaatac tcttatcgtg
    17281 gtggaacacg acgaagatac catgcgtgaa gctgattatc tgattgacgt tggtcctggt
    17341 gccggtgttt ttggtggtga gattgttgca gcaggtacac ctaagcaagt ggctcgcaac
    17401 agcaagtcta tcacaggtca gtacttgtca ggcaaacgtg tcatcccagt accagaagag
    17461 cgccgtgtcg gaaatggtcg ttttattgaa gtgacaggag cgcgtgagaa caacttgcaa
    17521 aatgtcacgg ctcgctttcc actaggaaaa tttatcgcag tgacaggtgt gtcgggttca
    17581 gggaaatcga ctctaatcaa cagcattctc aaaaaagcca ttgcccagaa gctcaaccgt
    17641 aattcagaca aacctggtaa gtttaagact attacaggga ttgagcatgt cgaccgtttg
    17701 attgatatta accagagccc tatcggacgg acgccgaggt ctaacccagc tacctataca
    17761 ggagtttttg acgatatacg tgaccttttt gctcagacaa atgaagccaa gattcgaggc
    17821 tacaagaagg ggcgcttcag tttcaacgtc aagggaggtc gctgtgaagc ttgctcaggt
    17881 gacgggatta tcaagattga gatgcacttc ttgccagatg tttatgtggc ttgtgaagtt
    17941 tgccacggaa cccgctataa cagtgaaacc ctagaagttc actacaagga aaagaatatt
    18001 tcgcaggtct tggatatgac ggtcaacgat gcggtagaat ttttccaaca cattccgaaa
    18061 attcaacgca aactccagac catcaaggat gtaggtctag gctatgtgac cttgggacag
    18121 ccagctacca ccctttctgg gggagaagcc caacgtatga agttagctag cgaactccac
    18181 aaacgctcga caggaaaatc tttctacatt ctggatgagc cgacgacagg gcttcataca
    18241 gaggacattg ctcgcctgct caaggtttta gctcgctttg tagacgccgg caatacagtc
    18301 ctcgtcatcg agcacaatct ggatgttatc aagacggcag accatatcat tgacttggga
    18361 cctgagggcg gtgtcggtgg tggaaccatc atcgtaacag gaactccaga agaagtagct
    18421 gccaacgaag ccagctatac aggacactat ttgaaaggaa agttacatca tgaataaacg
    18481 cgtacaagca tttctagcta aaatgcaaga aaaagaacta gatggtatca tcatcaacaa
    18541 tcttaaaaac gtctattatt tgactggttt ttggggctca aacggaacag tctttatcag
    18601 ccgtgaccgt caggtcttag tgacagactc tcgctatatc atcgcagcta agcaagaaac
    18661 cagtggtttt gagattgtgg ctgatcgtga tgaattggct gtcattgcag gaattgttaa
    18721 ggacatgaac ttgactcgta tcggttttga agatgagatt tcagtgtctt attaccaccg
    18781 tatgcaggca gcttttgcag gtttgaactt gcttccacaa actcagtttg tggaaggtct
    18841 tcgtatgatt aaggatgagg cagagattgc agcgattcgc aaggcttgtt ctatctcaga
    18901 ccaagctttc cgcgatgcgc ttgactttat caaaccagga aaaactgaaa ttgagattgc
    18961 caacttcctt gatttccgca tgcgtgagtt gggagcatct ggcttatctt ttgatacgat
    19021 cctagctagc ggtatcaatt cttctaaacc ccatgcccat ccaatgcaca aaccagtgga
    19081 gttgggagaa gccatcacca tggacttcgg ctgtctctat gaccactatg tcagtgatat
    19141 gacccggact atctatctag ggcatgttag cgatgagcag gcagagattt acaatacggt
    19201 tctaaaagct aaccaagcct tgattgacca agctaaggta ggcttaggtt tccgtgactt
    19261 tgacaaaatc cctcgtgata ttatcattga ggcaggttat ggtgactact ttactcacgg
    19321 cattggccac ggtattggtc tggatatcca tgaggaaccc tactttagtc agacttctac
    19381 agaaactatt aagacaggta tggccttgac cgatgaacca ggtatctata tcgaaggcaa
    19441 atatggcgtt cgtatcgagg atgatatcct gattacagag acaggttgtg aattattgac
    19501 cctagctcca aaagagttga tagtcattta gttgtttgtc aagaaaaaag tacgaaaatg
    19561 acgaaaaaag tcaaaaaaaa ttaaaaatag gtcgcaagtc ggatgttttt tatggtataa
    19621 tagactaaac tgatagtaac atgtagcgaa aggggtaggt acatgattaa aatttataca
    19681 gtctcaagtt gtactagctg taaaaaagca aaaacctggc tcaatgccca ccagttaagt
    19741 tataaagaac aaaaccttgg taaagaagga attacgagag aagaattact ggatattcta
    19801 accaaaacag ataacggaat agccagcatc gtttcgtcta aaaatcgcta tgccaaagcc
    19861 cttggagtgg atattgaaga tttgagtgtc aatgaagttc tcaatctgat tatggaaaca
    19921 ccgagaattt taaagagccc aatccttgta gatgaaaaac gcctgcaagt tggctacaag
    19981 gaagacgata ttcgtgcctt cctaccacgc tctgtccgta atgtagaaaa tgcagaagca
    20041 cgtttgcgtg cagctctata aacatcaagg ctgggagcac ttcccagtct tattctattt
    20101 taatttcaaa aagaaagaag aaagaaatga aaaaaatagt tcttgttagt ctagctttcc
    20161 tttttgtcct ggttggttgc ggacagaaaa aagaaactgg accagctaca aaaacagaaa
    20221 aagatacgct tcagtcggca ttgccagtta ttgaaaatgc tgagaagaat acagttgtaa
    20281 ctaagacttt ggtcttgccc aagtcagatg atggtagcca gcagacacaa acaattactt
    20341 acaaagacaa gacttttttg agtctaacta tccaacaaaa acgtccagtc tctgatgagt
    20401 tgaagactta tattgaccaa catggagtgg aggaaactca aaaagctctt cttgaagcgg
    20461 aggagaagga taagtctatc attgaagctc gtaaactggc aggtttcaaa cttgaaacaa
    20521 aactattgag cgcaacggaa cttcaaacaa cgactagttt tgattttcaa gttctggatg
    20581 tcaagaaggc ttcccagttg gaacatctga agaatattgg tttggaaaat cttttgaaaa
    20641 atgaaccaag caaatatatt tcagatagat tggcaaatgg cgcgacagaa caatagaaaa
    20701 tccaaataaa tagactgcct gaattgtaga aggtaaggaa ttatgctata atggtactat
    20761 tctaaggaaa gagggtgtta gaatgggatt tactgaagaa acagtacgtt ttaaattgga
    20821 cgattccaat aaaaaagaaa ttagcgaaac tttgacagat gtttatgctt cgttgaacga
    20881 taagggttac aacccaatta accaaatcgt aggttacgta ttgagtggag accctgccta
    20941 cgttcctcgt tataataatg cacgaaatca aatccgtaag tatgagcgtg atgaaatcgt
    21001 tgaggaattg gttcgctact acctcaaagg gcaaggagtc gatctataac ctatgagaat
    21061 tatgggattg gacgtcggtt caaaaacggt aggggtggcg attagcgatc cgcttggttt
    21121 tacagctcaa gggcttgaaa tcatccagat aaatgaagaa caaggcaaat ttggttttga
    21181 ccgcgttaag gaattggttg atacttacaa ggtggaacga tttgtagtgg gcttgcctaa
    21241 aaacatgaac aatacaagtg gaccgcgcgt agaagctagt caagcctacg gagcaaagct
    21301 agaagagttt tttggtttac cagtagacta tcaggatgaa cgcttgacaa cagtggctgc
    21361 tgagcgcatg ttgattgaac aagcagatat cagtcgcaat aagcgcaaga aagtcattga
    21421 taagttagca gctcagctga ttttacaaaa ttatttagat agaaaatttt aatataaagg
    21481 agaggctatg tcacacgatc ataaccacga ccacgaagaa cgtgaactaa tcacactagt
    21541 agatgagcaa ggaaatgaaa ccttgtttga aatccttttg acaattgatg gaaaagaaga
    21601 atttggtaaa aactatgttc ttctagtacc agttaacgca gaagaagacg aagacggaca
    21661 agttgaaatc caagcttact cattcattga aaatgaagat ggaacagaag gcgaattgca
    21721 accaatccca gaagactcag aagacgaatg gaacatgatt gaagaagtct tcaacagctt
    21781 tatggaggag tgatacagtc tgggagactg tatcagccca actcccagaa atggggaaga
    21841 gttggaacgt ccagtggatg tttttagccc agttccttta aataagagag aactggtagg
    21901 ttcgggggga cgtttttagc cctgcgctag aaattagaaa cgcaggtagt ctgagaaact
    21961 gtatcagccc aactcccaga aatggggaag ggttggaacg tccagtggat gttttactcc
    22021 acgttaatat tcatagttgc tagtgattag ctaaccaggt aagaggtcgg gacgaaaatc
    22081 ctggccttgt ttttattagt aaccatactt taatgcgata attgattgga cttttgtcaa
    22141 ggagatgtgt atgtttgaag tagaagaatg gctccatagt cggattggtt taaattttcg
    22201 atcaggtttg ggccgaatgc agcaagcggt ggatttgtta ggaaatcctg agcagtctta
    22261 ccctattatc cacgtaacag ggactaatgg gaaaggatct accattgctt ttatgaggga
    22321 attatttatg gggcatggca aaaaagttgc gacctttacc tcccctcata tcgtctctat
    22381 caatgaccga atctgcatta atgggcaacc tatagcagac gcagacttta tccgtttggc
    22441 tgatcaggtc aaggagatgg agaaaacgct tctgcaaact cctgaccagt tgtccttttt
    22501 tgaattgctg accttggttg cttttcttta ttttagggag caagaggtgg atttggtttt
    22561 attagaagtg ggaattggtg gcttacttga cacgaccaat gtggtaactg gagagcttgc
    22621 tgtcatcacc tccattgggc ttgatcatca agaaaccttg ggtgatagtc tagaagcaat
    22681 tgcagagcag aaagctggta ttttcaaggc tggtaagaag gcagtgattg cgaaattgcc
    22741 tccagaagct aggcttatct gtcagaaaaa agccgaatct ttagctgtta acctttatca
    22801 ggcaggtcaa gattttttaa tgctgaatgg tgatttttca agctctttac taaatctttc
    22861 acagctggac ataggcttag aaggagtcta tcagcaggag aatgcagcct tggcgttgca
    22921 aacttttctt ctttttatgg gagaaggaaa ggaagctgtt gatgaacagg ctgtaagaaa
    22981 ggccttggaa cagacccatt gggctggtcg cttggagcgt attcgcccac agatttactt
    23041 ggatggtgct cataacctcc ctgccttgac tcgcttggtt gagtttatca aagaaaaaga
    23101 gcaggaaggt tatcgacctc aaatcctctt tggatccttg aaacgtaagg attatcaagg
    23161 gatgttgggt tatctgactg aaaatttacc tcaagtggaa ctcaagatga ctggctttga
    23221 ctatcaaggg tctttggacg agacagatgt aacaggttac catgtaattc cctcttaccg
    23281 agaatttatc agcagtttcg aagaaagggc agacgctcaa gacttgctgt tcgtaacagg
    23341 gtctctctat tttatctcag aagtacgggg ctacctgctg gaccgtgagc agataaattg
    23401 accccctttt ttgaacttgt tatactaggg gaattgctgg ttagagaaaa tttctctaaa
    23461 ttggtagcag aaaggaaatc catcatgaaa ttaaaaagat tcacactttc tcttgcttct
    23521 ctagcaagtt ttagtctctt agtagcttgt tcacaaagag ctcaacaggt tcaacagcct
    23581 gctcaacaga ataccaatac tgcaaatgca gggggtaacc aaaatcaagc ggctccagta
    23641 caaaaccaac ctgttgctca gccgaccgat attgatggga cttatactgg tcaggatgac
    23701 ggagaccgta tcactttagt ggtaactgga acgactggta catggactga gctcgaatct
    23761 gacggggatc agaaagtcaa acaggttaca tttgattcag caaatcaacg catgattatt
    23821 ggcgatgatg tcaaaattta cactgtaaac ggtaatcaaa tcgtcgtaga tgatatggat
    23881 agagacccat cggaccaaat cgttttaact aaataagact atgcaagtag aaagggctgg
    23941 atttttcagc ctttttactt ttacctagaa aataatcata aatacttgcc ttctaccgaa
    24001 tacctgcgtt atactagtat caattacttg tttttagcat tcaaactatc aaggagggga
    24061 tatgaaatat aggaaatttc aattattgat gtccaagtat ggctttagtc tttcgattat
    24121 gttacttgaa ctttgtcttg tttttggtct ctttctttat ttaggacgca tggctcccat
    24181 tttatggatt actgtcctca ttctactgag tatcatcaca atcatttcga tagtcaaccg
    24241 taatacgact cctgagaata aggtaacctg gttgttagta gcctttgtgc cagtatttgg
    24301 tcccttgctc tatctgatgt ttggtgaaag gcgattgtcc aaaaaagaaa tcaaacaact
    24361 gaagaagcta ggctctatgc atttccaaga agcaaatagc cagctactaa aagagaaatt
    24421 aaaagaaagt gacaaggcag cttatggagt catcaagtcc ttattgagta tggataccaa
    24481 tgctgacatc tatgatcaaa ctgcctctac attttttcct aacggagaag ctatgtggaa
    24541 aaagatggta gaagatctta aaaaggctga gaaatttatt ttcttggaat attacattat
    24601 agaagaaggt ttgatgtgga atcgcatact agatatacta gagcaaaagg tagctcaggg
    24661 tgtagaggtt aagatgctct atgatgatat cggctgtatg gctactttaa caggagatta
    24721 tgcacatcga cttcgtcagc tgggcatcga ggcccataaa ttcaataaag ttattcctcg
    24781 tttgacagtg gcttataata acagagatca tagaaaaata ttgattgttg atggtcagat
    24841 agcctatact ggtggggtca atctggcaga tgagtacatt aaccacgtcg agagatttgg
    24901 ttattggaag gatagtggaa ttcgcttaga cggactagca gtaaaagctc tgacacgctt
    24961 atttttgacc acttggtaca ttaatcgagg agaaattagt gattttgatc aatatcattt
    25021 agaaaatcat tctatcccga gtgacggttt aaccattcca tacggaagtg gacccaagcc
    25081 aatttttcga gcgcaggtag ggaaaaaagt ttatcagagt ttaatcaatc aagcaacaga
    25141 atcggtctat attacgacac cttatttgat tatagattat gatttaacag agacaatcaa
    25201 aaatgcagct atgagagggg tcgatgttcg aattatcacc ccttacatac cagataagaa
    25261 gttcattcag ttagtcacga gaggagctta tcccgacctt ctttctgctg gtgttcggat
    25321 ttatgagtat agtccaggtt ttattcatag taagcagatg ttggtagacg aagattttgc
    25381 ggtggtgggg acaatcaatc tcgactaccg gagcttggta caccattatg aaaatgcagt
    25441 cttactctat aaaactcctt ctataaggga aatcgcccga gattttcgaa atatatttgc
    25501 agattctcag gaagtctatc ctcattctat caaaacgagc tggtatcaaa agcttgtaaa
    25561 agaaatcgcc cagctattcg cccctatctt ataagaaatc tagaacttag gatacaaacc
    25621 agacctattt ttcttgtctt ttacgaatag aaagaaagag gaggggaatt catgctaact
    25681 aaggaagaag tgaatgatat gattgagttc aaacagacac atttacaccg ctttcgggaa
    25741 attgaaacct ttgtgaaact atgcaaaaaa tcactcaaac agccatcgca agctgacaat
    25801 cctaggactt tttgatatac taagacagat aaattgaaca ggagaaacga tatgagtgtg
    25861 tatggtagag tagaagaagt tcataaggag aatcgtgaac ctctagaata ccaaatcgaa
    25921 caagaatcgc atcatcgtga atcaagtcgt cttcctttgg tgaaaatttt actatggagt
    25981 acgcttgtaa cggggataac tctaggagtg ccgctattac tcgacttaat gagtgcacaa
    26041 gaagtgcagg atttttatgc aggttgggcg cttcatcaga cagggaagat ttacagcgac
    26101 tattatggaa gtcaaggttt gctttattat ttgctgactt acgtgagtca gggcggattt
    26161 ttctttgcca tctttgagtg gttagccttg gtagcaggag gatttttcct ttttagatca
    26221 gcggacacct tgacagagca aggagaccaa gctggacatc tggtgactat tttttacatg
    26281 ctagttacag gtcttgcttt tggtggaggc tatgcgactc ttttagcgct tcctttctta
    26341 ttcgcagcct ttagtttagt tgcggcttac ctaagcaatc caagccatga taagggattt
    26401 gtacggattg ggctagcttt ggcaggcgga tttttctttg ctcccttatc atcgctcctg
    26461 tttattgctg tagtgagttt aggcttgttg gtctttaacc ttgggcatag acgttttgtg
    26521 catgggtttt atcagtttct tgcagtggct ttaggttttt cacttgtctt ttatccaact
    26581 gcctactata gtgctgcaac aggaagtttt ggggatgcga ttagtggtat tcgttatcct
    26641 attgacagta ttcgctttga ttttacttct aaaattttag agaatatgtt tttttatggc
    26701 ttgttgtccc ttggtttggg atttgtggtt tgtatctttt taggtctctt tcaatccaaa
    26761 ccctttaaac tatacgtcat ttcggttcct gcaagtcttg tggtaatttt aggtttgatt
    26821 ttacttttct tttcacaaga gcctctgcac gcttcttatt tgatggtcgt cttccctgtt
    26881 ttcctacttt tattggtaac caatattaag agtcaacaga gggggcgtag tgctagaaga
    26941 agccgaagag aaacgccagt tagcctatgg agtcgtttct tcaaaggaaa tctatatctg
    27001 ctagtttttg ggtttgtcta tcttttgtct gttccttttt tgatgaagtt tgtcctttat
    27061 ccagtacctt atcaagaacg taatcgtctt gctgatttgg taaaagagga gacaaatacg
    27121 gaagatgcta tctatgcatg ggatgatact gcgactcttt atcgtaagag tgagcgcttg
    27181 tcgccatcgg cgattttgtc cccgttgcac tatacagcaa ctgaggaaaa tcgtaataag
    27241 ttacttaatg acttgaaaga aaaacaacct aaggtgattg tggtaaatga caaggtggta
    27301 gtctggtctg aagtggaaac actcttaaaa gaaaattacc aacaagtaaa gactgattac
    27361 tcagagttta aagtctataa aattaaataa ccaaatcaat atcttgtgta tttttaaaaa
    27421 ttttaggatt tttaacacaa gatattgatt tttcttttta gagtggtata atacttttta
    27481 gaaagaacat tttagaaaag agcatgcgta tgattgcact agaagaaaaa attacaattt
    27541 tgccaactct cttcgtcgag aaacgagatg ggagacgtgt tgtatttgat gtggacaaga
    27601 ttgacaaggc tctccacaag gcggctgaca aggttatgga tgtgacaccc ctggttgaaa
    27661 aacgcctcaa tgatctgact gagcgaatta ttacagaaat tcatagtcgc tttccacagg
    27721 gaattaagat ttacgaaatt caaaatatcg tagaacatga actccttgaa gccaaagaat
    27781 atgcgctggc tgaggagtat attacttatc ggacacagag ggattttgag cgctcaaaag
    27841 cgacggatat caactttagt attcataaac ttctcaacaa agaccagaca gttgtcaatg
    27901 aaaatgctaa taaagacagt gatgtcttta acactcagcg tgatttgaca gcagggattg
    27961 ttgggaaatc aatcggactg caaatgcttc ctaagcacgt agccaatgcc caccaaaagg
    28021 gggatatcca ctatcacgat ttggactaca gtccctatac ccctatgacc aactgctgtt
    28081 tggttgattt taagggtatg ttggaaaatg gttttaagat tggaaatgca gaggtagaga
    28141 gtcccaagtc tatccagact gcgacagcac agatttctca aatcattgcc aacgttgctt
    28201 ctagccagta cggtggctgt tcagctgacc gtatcgatga aattttggcg ccttatgcag
    28261 agaagaacta tcaaaaacat ctcaaagatg cagaagagtg ggtattgcct gaaaaacggg
    28321 aagattacgc ttggaagaaa acgcaaaagg acatctacga tgccatgcaa tctcttgagt
    28381 atgaaatcaa tactctcttc acttcaaatg gacaaacacc ttttacttcg ttaggttttg
    28441 gtctgggaac cagtcgtttt gaacgagaaa ttcaaaaagc tattttaaac attcgaatca
    28501 agggtcttgg ttcagaacac cgtacggcta tctttcctaa acttattttc actctgaaaa
    28561 gaggccttaa tttagaggaa ggaactccca actacgacat caagcagttg gctcttgagt
    28621 gtgcaaccaa acggatgtat ccagatgttt tatcttacga taagattgtt gacttgacag
    28681 gatccttcaa ggtgcctatg ggttgccgtt ctttcctcca aggatggaag gatgaaaatg
    28741 gtgtagaagt caattcaggt cgcatgaatc tgggtgttgt gacggttaat ctgcctcgta
    28801 ttgctcttga gtctgaaggt gatatgaata agttctggga aatcttcaac gagcgaatga
    28861 atatcgcaga agatgctctt gtttaccgtg tcgaacgcac taaagaggcg acaccagcaa
    28921 atgctcctat tctttatcag tacggtgctt ttggccatcg tctaggtaaa gaagaaagtg
    28981 ttgaccagct ctttaagaat cgtcgtgcga ccgtttcgct gggctatatc ggcttgtatg
    29041 aagtagcgac agttttcttt ggtaacagct gggaaagtaa tccagatgct aaggaattca
    29101 cgctagacat cattcacgat atgaaacgcc gtgtagaaga gtggtcagac caatatggct
    29161 accatttctc tatctactca acaccatccg aaagtctgac agaccgtttc tgccgactag
    29221 atatagacaa gtttggctct attcctgata tcacagacaa ggaatactac accaactctt
    29281 tccactacga tgttcgtaaa aatccaacac cgtttgaaaa attggacttt gagaaagtct
    29341 atccagaagc aggtgcgtca ggtggtttca tccattattg tgagtatcca gtccttcagc
    29401 aaaatccaaa ggccttggaa gctgtctggg attatgctta tgaccgtgta ggctatctag
    29461 gcaccaatac tccgattgac cgttgctaca agtgtgactt tgaaggggat tttgaaccaa
    29521 ctgagagagg gtttgcttgt ccaaactgtg gcaatagcga tcctaaaaca gtagacgttg
    29581 ttaaacggac ttgtggctat cttggcaatc ctcaagcaag accgatggtc aacgggcgtc
    29641 acaaggaaat cgctgcgcgt gtcaaacata tgaatggttc aacgattaaa atagctgggc
    29701 atcaagtaac aaattagaaa gaaatgaaat ggggaaatat caactagacg ataaggggcg
    29761 cgcacaagtg acccgttatc acgagaaata ctctaaaggt ggagctggta agaaagaacg
    29821 cttgcttagc ttcagagaac aatttttaaa caagaacaag aaaaaataaa agtgagagcc
    29881 agctctcgct tttctcatag tgggaggtaa ggatggaatt acgcagacca agattagcgg
    29941 ataagaaagc tgttttagat atgatgacag agtttgaaaa gagccaatca gcccatgatg
    30001 gaggattttg ggatacagag aactttgtgt atgaagagtg gttggaaagc aatcaggaac
    30061 aggaaatggg gattaatctg cctgaaggat gggttcctgc aattcagtta gtggcttttt
    30121 ctgagaaagg tcaagcagtt ggatttctta atctccggtt gcgcctcagt aactttctac
    30181 tagaagaagg tgggcacatt ggctactcca tccgtccatc tgaaagaggc aagggttatg
    30241 caaaagagac tctccgtcag ggcttgcaag ttgctaagga aaagaacatc aagaaagctc
    30301 tggtgacctg tagcgtgaat aatcctgcta gcagagcagt cattctagca aatggtggaa
    30361 tatttgagga tgctcacaat ggagtcgagc gttattggat agaggtagcg aatgaataat
    30421 ccaaaaccac aagaatggaa aagcgaggaa cttagtcaag gtcgtatcat tgactacaag
    30481 gcctttaact ttgtggacgg cgaaggcgtg cgcaactctc tctatgtatc aggctgtatg
    30541 tttcactgcg agggatgtta taatgttgcg acttggtctt ttaatgctgg cattccctat
    30601 acagcagaat tagaagagca gatcatggca gaccttgccc aaccctatgt tcaaggcttg
    30661 actttgctgg gaggggaacc ttttctcaat actggcattc tcttgcctct agttaaacgc
    30721 atccgaaagg aattgtcaga caaggacatt tggtcctgga cgggctacac ttgggaagaa
    30781 atgatgttgg aaactccaga taaactggaa ctcttatcat tgattgacat tcttgtcgat
    30841 ggacgatacg atcgaactaa gagaaatctc atgctccagt ttcgaggttc gtccaaccaa
    30901 cgaattatcg atgtgcaaaa atcgctcaaa agtgggcaag tagtgatttg ggacaagctc
    30961 aatgacggaa aagaaagcta tgaacaggtg aagagagaat gaagaaaaaa gacttagtag
    31021 atcaactagt ctcagagatc gagacgggga aagtcaggac actgggaata tacggtcatg
    31081 gagcttcagg taaatcaacc tctgcacagg aattgtacca agctttagat tctactacag
    31141 taaatttgct agaaacagat ccctatatca cctcaggacg ccatctggta gtacccaagg
    31201 acgcgccgaa tcaaaaggtg acagccggtc tgccagtggc gcatgaactg gagagtttgc
    31261 agagagatat ccttgccttg caggcgagta tggatgtctt gacaattgaa gaaccttgga
    31321 aggctagtga ggtcttgtct ggagccaaac caattttgat tgtcgaaggg atgtctgttg
    31381 gctttctacc caaggaactc tttgaaaaaa ccatctgttt ctacacggat gaggagaccg
    31441 aattaaagcg acgccttgct agagatacga ctgtgagaaa tcgtgatgca tcctttatat
    31501 tggctagcca tcagatgaga cgggagcagt atctgcgata ctataaagaa actgagtcta
    31561 aggcggatat cttagtggat caatcagaag ataaatttga tgtcaagagg acttaactta
    31621 tgtatagaag aaatatagaa gaaaatccct aattttagaa agaaaaagta ggaaaacagt
    31681 ttcatcccaa aaaaacgaaa aaacctaaca aatcccttgc aatcgcaggg gatttgtgtt
    31741 attctattat ggtgctgtaa attacagcct tagttttgat gcaagaggtt gcgacacgct
    31801 cggttgcatt gccacgcaac accgtgtcgg ttttcttgtg gagctagcct attatcttaa
    31861 atagacgaaa aggagaaaaa gatggcaaac aaaaaaatcc gtatccgttt gaaagcttac
    31921 gaacaccgta cgcttgacac agcggctgca aaaatcgtag aatcagctac tcgtacaggt
    31981 gcacaagttg cgggtccaat cccacttcca actgaacgta gcctctacac aatcattcgt
    32041 gcgactcaca aatacaaaga ctctcgcgaa caatttgaaa tgcgtacaca caaacgtttg
    32101 atcgatatcg ttaacccaac tcaaaaaaca gttgatgcct tgatgaaatt ggatcttcca
    32161 agtggtgtaa acgtagaaat caaactttaa tctaaaatat aagagagcag aggctggtgt
    32221 ttcaatctaa ttgaacacgg gctaaacttg gtgtgaaaaa gataaacttc ctcgcgtctg
    32281 ctagacactg tgtcagtttc ctattttcac gttgagtttg acgccctttg tatcttagac
    32341 ttgagcataa aaaacgctcg ttaaaaactt tttgaataaa aaatatagaa aaggaactat
    32401 tttctcatga caaaaggaat cttagggaaa aaagtgggaa tgactcaaat cttcactgaa
    32461 gctggcgaat tgatccctgt aacagttatt gaagcaactc caaacgttgt tcttcaagtt
    32521 aaaactgttg aaacagacgg atacaacgct atccaagttg gtttcgatga caaacgcgaa
    32581 gtattgagca acaaacctgc taaaggacat gtagcgaaag ctaacacggc tcctaagcgc
    32641 ttcattcgtg aattcaaaaa cgttgaaggc ttggaagttg gtgctgaaat tacagttgaa
    32701 acattcgcag ctggagacgt tgttgacgta actggtactt ctaaaggtaa aggtttccaa
    32761 ggtgttatca aacgccacgg acaatcacgt ggaccaatgg ctcacggttc tcgttaccac
    32821 cgtcgtccag gttctatggg acctgttgca cctaaccgcg tattcaaagg taaaaacctt
    32881 gcagggcgta tgggtggcga ccgcgtaaca attcaaaacc ttgaagttgt acaagttgtt
    32941 ccagaaaaga acgttatcct tatcaaaggt aacgtaccag gtgctaagaa atctcttatc
    33001 actatcaaat cagcagttaa agctggtaaa taataaagaa aggggaaatc agtcacaatg
    33061 gcaaacgtaa cattatttga ccaaactggt aaagaagctg gccaagttgt tcttaacgat
    33121 gcagtatttg gtatcgaacc aaatgaatca gttgtgtttg atgtaatcat cagccaacgc
    33181 gcaagccttc gtcaaggaac acacgctgtt aaaaaccgct ctgcagtatc aggtggtgga
    33241 cgcaaaccgt ggcgtcaaaa aggaactgga cgtgctcgtc aaggttctat ccgctcacca
    33301 caatggcgtg gtggtggtgt tgtcttcgga ccaactccac gttcatacgg ctacaaactt
    33361 ccacaaaaag ttcgtcgcct agctcttaaa tcagtttact ctgaaaaagt tgctgaaaac
    33421 aaattcgtag ctgtagacgc tctttcattt acagctccaa aaactgctga atttgcaaaa
    33481 gttcttgcag cattgagcat cgattctaaa gttcttgtta tccttgaaga aggaaatgaa
    33541 ttcgcagctc tttcagctcg taaccttcca aacgtgaaag ttgcaactgc tacaactgca
    33601 agtgttcttg acatcgcaaa tagcgacaaa cttcttgtca cacaagcagc tatctctaaa
    33661 atcgaggagg ttcttgcata atgaatttgt atgatgttat caaaaaacct gtcatcactg
    33721 aaagctcaat ggctcaactt gaagcaggaa aatatgtatt tgaagttgac actcgtgcac
    33781 acaaactttt gatcaagcaa gctgttgaag ctgctttcga aggtgttaaa gttgccaatg
    33841 ttaacacaat caacgtaaaa ccaaaagcta aacgtgttgg acgttacact ggttttacta
    33901 acaaaactaa aaaagctatc atcacactta cagctgattc taaagcaatc gagttgtttg
    33961 ctgctgaagc tgaataatct aaggaggaaa tatcgtggga attcgtgttt ataaaccaac
    34021 aacaaacggt cgccgtaata tgacttcttt ggatttcgct gaaatcacaa caagcactcc
    34081 tgaaaaatca ttgcttgttg cattgaagag caaggctggt cgtaacaaca acggtcgtat
    34141 cacagttcgt caccaaggtg gtggacacaa acgtttctac cgtttggttg acttcaaacg
    34201 taataaagac aacgttgaag cagttgttaa aacaatcgag tacgatccaa accgttctgc
    34261 aaacatcgct cttgtacact acactgacgg tgtgaaagca tacatcatcg ctccaaaagg
    34321 tcttgaagta ggtcaacgta tcgtttcagg tccagaagca gatatcaaag tcggaaacgc
    34381 tcttccactt gctaacatcc cagttggtac tttgattcac aacatcgagt tgaaaccagg
    34441 tcgtggtggt gaattggtac gtgctgctgg tgcatctgct caagtattgg gttctgaagg
    34501 taaatacgtt cttgttcgtc ttcaatcagg tgaagttcgt atgattcttg gaacttgccg
    34561 tgctacagtt ggtgttgtcg gaaacgaaca acatggactt gtaaaccttg gtaaagcagg
    34621 acgtagccgt tggaaaggta tccgcccaac agttcgtggt tctgtaatga accctaacga
    34681 tcacccacac ggtggtggtg aaggtaaagc accagttggt cgtaaagcac catctactcc
    34741 atggggcaaa cctgctcttg gtcttaaaac tcgtaacaag aaagcgaaat ctgacaaact
    34801 tatcgttcgt cgtcgcaacg agaaataata ttaaactagt cgcttaagca actagtaaat
    34861 ccgccagctc ggtagcgctc cataggagtg caagccgctg tggtacaaca tttaaaggag
    34921 aaaatataaa aatgggacgc agtcttaaaa aaggaccttt cgtcgatgag catttgatga
    34981 aaaaagttga agctcaagct aacgacgaaa agaaaaaagt tattaaaact tggtcacgtc
    35041 gttcaacgat cttcccaagt ttcattggtt acactattgc agtttatgac ggacgtaaac
    35101 acgtacctgt ttacatccaa gaagacatgg taggccacaa acttggtgaa tttgcaccaa
    35161 ctcgtactta caaaggtcac gctgcagacg acaagaaaac acgtagaaaa taaggagaac
    35221 ataaatggca gaaattactt cagctaaagc aatggctcgt acagtacgtg tttcacctcg
    35281 taaatcacgt cttgttcttg ataacatccg tggtaaaagc gtagccgatg caatcgcaat
    35341 cttgacattc actccaaaca aagctgctga aatcatcttg aaagttttga actcagctgt
    35401 agctaacgct gaaaacaact ttggtttgga taaagctaac ttggtagtat ctgaagcatt
    35461 cgcaaacgaa ggaccaacta tgaaacgttt ccgtccacgt gcgaaaggtt cagcttcacc
    35521 aatcaacaaa cgtacagctc acatcactgt agctgttgca gaaaaataag gaggtaaaat
    35581 cgtgggtcaa aaagtacatc caattggtat gcgtgtcggc atcatccgtg attgggatgc
    35641 caaatggtat gctgaaaaag aatacgcgga ttaccttcat gaagatcttg caatccgtaa
    35701 attcgttcaa aaagaacttg ctgacgcagc agtttcaact attgaaatcg aacgcgcagt
    35761 aaacaaagtt aacgtttcac ttcacactgc taaaccaggt atggttatcg gtaaaggtgg
    35821 tgctaacgtt gatgcactcc gtgcaaaact taacaaattg actggaaaac aagtacacat
    35881 caacatcatc gaaatcaaac aacctgattt ggatgctcac cttgtaggtg aaggaattgc
    35941 tcgtcaattg gagcaacgtg ttgctttccg tcgtgcacaa aaacaagcaa tccaacgtgc
    36001 aatgcgtgct ggagctaaag gaatcaaaac tcaagtatca ggtcgtttga acggtgcaga
    36061 tatcgcccgt gctgaaggat actctgaagg aactgttccg cttcacacac ttcgtgcaga
    36121 tatcgattac gcttgggaag aagcagatac tacatacggt aaacttggtg ttaaagtatg
    36181 gatctaccgt ggtgaagttc ttccagctcg taaaaacact aaaggaggta aataaccaat
    36241 gttagtacct aaacgtgtta aacaccgtcg tgaattccgt ggaaaaatgc gcggtgaagc
    36301 aaaaggtgga aaagaagtag cattcggtga atacggtctt caagctacaa ctagccactg
    36361 gatcactaac cgccaaatcg aagctgctcg tatcgccatg actcgttaca tgaaacgtgg
    36421 tggtaaagtt tggattaaaa tcttcccaca caaatcatac actgctaaag ctatcggtgt
    36481 gcgtatggga tctggtaaag gggcacctga aggttgggta gcaccagtta aacgtggtaa
    36541 agtgatgttc gaaatcgctg gtgtatctga agagattgca cgtgaagcgc ttcgacttgc
    36601 tagccacaaa ttgccagtta aatgtaaatt cgtaaaacgt gaagcagaat aaggagaagg
    36661 catgaaactt aatgaagtaa aagaatttgt taaagaactt cgtggtcttt ctcaagaaga
    36721 actcgcgaag cgcgaaaacg aattgaaaaa agaattgttt gaacttcgtt tccaagctgc
    36781 tactggtcaa ttggaacaaa cagctcgctt gaaagaagtt aaaaaacaaa tcgctcgtat
    36841 caaaacagtt caatctgaag cgaaataata gactagggaa ggagaaattt caatggaacg
    36901 caataatcgt aaagttcttg ttggacgtgt tgtatctgac aaaatggaca agacaatcac
    36961 agttgtagtt gaaacaaaac gtaaccaccc agtctatggt aaacgtatta actactctaa
    37021 aaaatacaaa gctcatgatg aaaacaatgt tgccaaagaa ggcgatatcg tacgtatcat
    37081 ggaaactcgc ccgctttcag ctacaaaacg tttccgtctt gtagaagttg ttgaagaagc
    37141 ggtcatcatc taatcaaacc tgaaaggaga aaactgaaat gattcaaaca gaaactcgtt
    37201 tgaaagtcgc agacaacagc ggtgctcgcg aaatcttgac tatcaaagtt cttggtggtt
    37261 caggacgtaa atttgcaaac atcggtgatg ttatcgtggc atctgtaaaa caagctactc
    37321 ctggtggtgc ggttaaaaaa ggtgacgttg ttaaagcagt tatcgttcgt actaaatcag
    37381 gtgctcgtcg tgctgatggt tcatacatca aatttgacga aaacgcagca gttatcatcc
    37441 gtgaagacaa aactcctcgc ggaacacgta tctttggccc agttgcacgt gaattgcgtg
    37501 aaggtggctt catgaagatc gtgtcacttg ctccagaagt actttaattt ttaggaacaa
    37561 actagtcccc tagcttcaag ctagggtgcc cttatgggcg taagaaaaat caaggagaaa
    37621 cctaatgttt gtaaaaaaag gcgacaaagt tcgcgtaatc gctggtaaag ataagggaac
    37681 agaagctgtt gtccttactg cccttccaaa agtaaacaaa gttatcgttg aaggtgttaa
    37741 cattgttaag aaacaccaac gtccaactaa cgagcttcct caaggtggta tcatcgagaa
    37801 agaagcagct atccacgtat caaacgttca agttttggac aaaaatggtg tagctggtcg
    37861 tgttggttac aaatttgtag acggtaaaaa agttcgctac aacaaaaaat caggcgaagt
    37921 gcttgattaa tcacgaagga aaggagaagt ataatggcaa atcgtttaaa agaaaaatat
    37981 cttaatgaag tagttcctgc tttgacagaa caattcaact actcatcagt gatggctgtg
    38041 cctaaagtag ataagattgt tttgaacatg ggtgttggtg aagctgtatc aaacgctaaa
    38101 agccttgaaa aagctgctga agaattggca cttatctcag gtcaaaaacc acttatcact
    38161 aaagctaaaa aatcaatcgc cggcttccgt cttcgtgaag gtgttgcgat cggtgcaaaa
    38221 gttacccttc gtggtgaacg tatgtacgaa ttcttggata aattggtatc agtttcactt
    38281 ccacgtgtac gtgacttcca cggtgtccca acaaaatcat ttgatggacg cgggaactac
    38341 acacttggtg tgaaagaaca attaatcttc ccagaaatca acttcgatga cgttgacaaa
    38401 actcgtggtc ttgacatcgt tatcgtaaca actgctaaca ctgacgaaga gtcacgtgca
    38461 ttgcttacag gccttggaat gccttttgca aaataatata ggaggtaaat ctaatggcta
    38521 aaaaatcaat ggtagctaga gaggctaaac gccaaaaaat tgttgaccgt tatgctgaaa
    38581 aacgtgctgc attaaaggcg gcaggggact acgaaggttt atctaaatta cctcgcaacg
    38641 cctcaccgac tcgtttacat aatcgttgta gggttacggg gcgcccacat tcagtttacc
    38701 gcaaatttgg tctgagtcgt atcgcttttc gcgaacttgc gcataaaggt caaattcctg
    38761 gtgtaacaaa ggcatcttgg taatttaaga tatcaagagc gtcaaaactc caagtaaaaa
    38821 taggaaactt gacgaagaaa ctaaagtttc taggaaagtt tatctttttc acacagagtt
    38881 tagcccgggt tcaattgggc ttgccaattt gaacacgagc tacagctttg gcaaaaaaga
    38941 ccaatttgct ttggagcatt gcttctgcat taaattgtct atttttgctc gtgctgttac
    39001 gctctttgta tcatgtatta actagcaagt gcaacttgca aactactagt aagaggagaa
    39061 aaacaaaatg gttatgactg acccaatcgc agacttccta actcgtattc gtaatgctaa
    39121 ccaagctaaa cacgaagtac ttgaagtacc tgcatcaaac atcaaaaaag ggattgctga
    39181 aatccttaaa cgcgaaggtt ttgtaaaaaa cgttgaaatc attgaagatg acaaacaagg
    39241 cgtcatccgt gtatttctta aatacggacc aaatggtgag aaagttatca ctaacttgaa
    39301 acgtgtttct aaaccaggac ttcgtgtcta caaaaaacgt gaagaccttc caaaagttct
    39361 taacggactt ggaattgcca tcctttcaac ttctgaaggt ttgcttactg ataaagaagc
    39421 acgccaaaag aatgttggtg gtgaggttat cgcttacgtt tggtaaaatc aagatacaaa
    39481 gctcgtaaag aacaaagcaa aattaggaag ttggagaagt ttgtttacaa acaagccaac
    39541 ttatctattt tgcacagttc ttagagcgtg ttcagttcag ctcttgaact aaataagtat
    39601 ctgaaccccg tgaaaactgg ccgttctggc ctgacaattt aacaggagaa aataaacatg
    39661 tcacgtattg gtaataaagt tatcgtgttg cctgctggtg ttgaactcgc taacaatgac
    39721 aacgttgtaa ctgtaaaagg acctaaagga gaacttactc gtgagttctc aaaagatatt
    39781 gaaatccgtg tggaaggtac tgaagtaact cttcaccgtc caaacgattc aaaagaaatg
    39841 aaaactatcc acggaactac tcgtgccctt ttgaacaaca tggttgttgg tgtatcagaa
    39901 ggattcaaga aagaacttga aatgcgtggg gttggttacc gtgcacagct tcaaggatct
    39961 aaacttgttt tggctgttgg taaatctcat ccagacgaag ttgaagctcc agaaggaatt
    40021 acttttgaac ttccaaaccc aacaacaatc gttgttagcg gaatttcaaa agaagtagtt
    40081 ggtcaaacag ctgcttacgt acgtagcctt cgttcaccag aaccatataa aggtaaaggt
    40141 atccgttacg ttggtgaatt cgttcgccgt aaagaaggta aaacaggtaa ataatgttga
    40201 gtggttgatc atcaaccacc aacctatttt ccaactttgt gcatagcaca cgatttaaaa
    40261 ctaaagaggt gaaaactgtg atttcaaaac cagataaaaa caaactccgc caaaaacgcc
    40321 accgtcgcgt tcgcggaaaa ctctctggaa ctgctgatcg cccacgtttg aacgtattcc
    40381 gttctaatac aggcatctac gctcaagtga ttgatgacgt agcgggtgta acgctcgcaa
    40441 gtgcttcaac tcttgataaa gaagtttcaa aaggaactaa aactgaacaa gccgttgctg
    40501 tcggtaaact cgttgcagaa cgtgcaaacg ctaaaggtat ttcagaagtg gtgttcgacc
    40561 gcggtggata tctatatcac ggacgtgtga aagctttggc tgatgcagct cgtgaaaacg
    40621 gattgaaatt ctaataggag gacactagaa aatggcattt aaagacaatg cagttgaatt
    40681 agaagaacgc gtagttgctg tcaaccgtgt tacaaaagtt gttaaaggtg gacgtcgtct
    40741 tcgtttcgca gctcttgttg ttgttggtga ccacaatggt cgcgtaggat ttggtactgg
    40801 taaagctcaa gaagttccag aagcaatccg taaagcagta gatgatgcta agaaaaactt
    40861 gatcgaagtt cctatggttg gaacaacaat cccacacgaa gttctttcag aattcggtgg
    40921 agctaaagta ttgttgaaac ctgctgtaga aggttctgga gttgccgctg gtggtgcagt
    40981 tcgtgccgtt gtggaattgg caggtgtggc agatattaca tctaaatcac ttggttctaa
    41041 cactccaatc aacattgttc gtgcaactgt tgaaggtttg aaacaattga aacgcgctga
    41101 agaaattgct gcccttcgtg gtatttcagt ttctgatttg gcataagaaa ggggataaaa
    41161 tggctcaaat taaaattact ttgactaagt ctccaatcgg acgcattcca tcacaacgta
    41221 aaactgttgt agcacttgga cttggcaaat tgaacagctc tgttattaaa gaagataacg
    41281 ctgctatccg tggtatgatt acagcagtat ctcacttagt aacagttgaa gaagtaaact
    41341 aatgaagttt taggggatgt gcactgtacc atcccctaaa actagatata gtcatctatg
    41401 atgacatcgt ataggcgagt tgatggggga gacaaccttt tctcccttat cggcgctagc
    41461 attttacaaa agaggagaaa ataaaaatga aacttcatga attgaaacct gcagaaggtt
    41521 ctcgtaaagt acgtaaccgc gttggtcgtg gtacttcatc aggtaacggt aaaacatctg
    41581 gtcgtggtca aaaaggtcaa aaagctcgta gcggtggcgg agttcgcctt ggttttgaag
    41641 gtggacaaac tccattgttc cgtcgtcttc caaaacgtgg attcactaac atcaacgcta
    41701 aagaatacgc aattgtgaac cttgaccaat tgaacgtctt tgaagatggt gctgaagtaa
    41761 ctccagttgt tcttatcgaa gcaggaattg ttaaagctga aaagtcaggt attaaaattc
    41821 ttggtaacgg tgagttgact aagaaattga ctgtgaaagc agctaaattc tctaaatcag
    41881 ctgaagaagc tatcactgct aaaggtggtt cagtagaagt catctaagag aggtgaccta
    41941 tgttttttaa attattaaga gaagctctta aagtcaagca ggttcgatca aaaattttat
    42001 ttacaatttt tatcgttttg gtctttcgta tcggaactag cattacagtt cctggtgtga
    42061 atgccaatag cttgaatgct ttaagtggat tatccttctt aaacatgttg agcttggtgt
    42121 cggggaatgc cctaaaaaac ttttcgattt ttgccctagg agttagtccc tatatcaccg
    42181 cttctattgt tgtccaactc ttgcaaatgg atattttacc caagtttgta gagtggggta
    42241 aacaagggga agtaggtcga agaaaattga atcaagctac tcgttatatt gctctagttc
    42301 tcgcttttgt gcaatctatc gggattacag ctggttttaa taccttggct ggagctcaat
    42361 tgattaaaac tgctttaact ccacaagttt ttctgacgat tggtatcatc ttaacagctg
    42421 gtagtatgat tgtcacttgg ttgggtgagc aaattacaga taagggatac ggaaacggtg
    42481 tttccatgat tatctttgcc gggattgttt cttcaattcc agagatgatt cagggcatct
    42541 atgtggacta ctttgtgaac gtcccaagta gccgtatcac ttcatctatc attttcgtaa
    42601 tcattttgat tattactgta ttgttgatta tttactttac aacttatgtt caacaagcag
    42661 aatacaaaat tccaatccaa tatactaagg ttgcacaagg tgctccatct agctcttacc
    42721 ttccgttaaa ggtaaaccct gctggagtta tccctgttat ctttgccagt tcgattactg
    42781 cagcgcctgc ggctattctt cagtttttga gtgccacagg tcatgattgg gcttgggtaa
    42841 gggtagcaca agagatgttg gcaactactt ctccaactgg tattgccatg tatgctttgt
    42901 tgattattct ctttacattc ttctatacgt ttgtacagat taatcctgaa aaagcagcag
    42961 agagcctaca aaagagtggt gcctatatcc atggagttcg tcctggtaaa ggtacagaag
    43021 aatatatgtc taaacttctt cgtcgtcttg caactgttgg ttccctcttc cttggtgtga
    43081 tttccatttt accgattgca gctaaagatg tatttggtct ttctgatgtt gttgcctttg
    43141 gtggaacaag tctcttgatc attatctcta caggtatcga aggaatcaag caattggaag
    43201 gttacctatt gaaacgtaag tatgttggtt ttatggacag aacagaataa aagtatttac
    43261 tgaatcagta aatgctgaga gagtggaggt ttaaactctg acatttgtaa gagttggatc
    43321 tcccctcttc tattttgttt ttaaataggg gtgaaaagac tttttgcttc tatttaaaaa
    43381 taaaataagg agatcaaatc atgaatcttt tgattatggg cttacctggt gcaggtaagg
    43441 gaactcaagc agcaaaaatc gtagaacaat tccatgttgc acatatctca acaggtgata
    43501 tgttccgcgc tgcaatggca aatcaaactg aaatgggtgt tcttgctaag tcatatattg
    43561 acaagggtga attggttcct gacgaagtta caaatggaat cgtaaaagaa cgtctttcac
    43621 aagatgatat taaagaaaca ggattcttat tggatggtta cccacgtaca attgaacaag
    43681 ctcatgcctt ggacaaaaca ttggctgaac ttggcattga actagaaggt gttatcaata
    43741 ttgaagtgaa ccctgacagc cttttggaac gtttgagtgg gcgtatcatc caccgcgtaa
    43801 ctggagaaac tttccacaag gtctttaacc caccagttga ctataaagaa gaagattact
    43861 accaacgtga agatgataag cctgagacag taaaacgtcg tttggatgtt aatattgctc
    43921 aaggagaacc aatcattgct cactaccgtg ccaaaggttt ggttcatgac atcgaaggta
    43981 atcaaaatat caatgatgtc ttctcagata ttgaaaaagt attgacaaat ttgaaataaa
    44041 gcgtttttca cacttgcaaa aatccgctac aaatgttata ctgagatagt ctgacttata
    44101 attgttgtct ctgtgtctag aggcatcgaa tcgaaattta tggaggtgct tttgcgtggc
    44161 aaaagacgat gtgattgaag ttgaaggcaa agtagttgat acaatgccga atgcaatgtt
    44221 tacggttgaa cttgaaaatg gacatcagat tttagcaaca gtttctggta aaattcgtaa
    44281 aaactatatt cgtattttag cgggagatcg tgttactgtc gaaatgagtc catatgactt
    44341 gacacgtgga cgtatcactt accgctttaa ataatcgaaa aacttggagg gataagaaat
    44401 gaaagtaaga ccatcggtca aaccaatttg cgaatactgt aaagttattc gtcgtaatgg
    44461 tcgtgttatg gtaatttgcc cagcaaatcc aaaacacaaa caacgtcaag gataagatag
    44521 aaaggagaaa acatggctcg tattgctgga gttgatattc caaatgacaa acgcgtagta
    44581 atctcattga cttatgttta tggtatcgga cttgcaacat ctaagaaaat tttggctgct
    44641 gctggaatct cagaagatgt tcgtgtacgt gatcttacat cagatcaaga agatgctatc
    44701 cgtcgtgaag tggatgcaat caaagttgaa ggtgaccttc gtcgtgaagt aaacttgaac
    44761 atcaaacgtt tgatggaaat cggttcatac cgtggtatcc gtcaccgtcg tggacttcct
    44821 gtccgtggac aaaacactaa aaacaacgct cgcactcgta aaggtaaagc tgttgcgatt
    44881 gctggtaaga aaaaataata taggaggtaa aagtcttggc taaaccaaca cgtaaacgtc
    44941 gtgtgaaaaa gaatatcgaa tctggtattg ctcatattca cgctacattt aataacacta
    45001 ttgttatgat tactgatgtg catggtaatg caattgcttg gtcatcagct ggtgctcttg
    45061 gtttcaaagg ttctcgtaaa tctacaccat tcgctgctca aatggcttct gaagctgctg
    45121 ctaaatctgc acaagaacac ggtcttaaat cagttgaagt tactgtaaaa ggtccaggtt
    45181 ctggtcgtga gtcagctatt cgtgcgcttg ctgccgctgg tcttgaagta acagcaattc
    45241 gtgatgtgac tccagtgcca cacaatggtg ctcgtcctcc aaaacgtcgc cgtgtataat
    45301 catcgcatta cactgctttt cgtttaagag ggagtaacta aatgatcgag tttgaaaaac
    45361 caaatataac aaaaattgat gaaaataaag attatggcaa gtttgtaatc gaaccacttg
    45421 aacgtggcta cggtacaact cttggtaact ctcttcgtcg tgtacttcta gcttctctac
    45481 caggagcagc tgtgacatct atcaacattg atggtgtgtt acatgagttt gacacagttc
    45541 caggtgttcg tgaagacgtg atgcaaatca ttctgaacat taaaggaatt gcagtgaaat
    45601 cgtacgttga agacgaaaaa atcatcgaac tggatgttga aggtcctgct gaagtaacag
    45661 ctggtgacat tttgacagat agcgatattg aaattgtaaa tccagatcat tatctcttta
    45721 caatcggtga aggttcttct ctaaaagcga ctatgactgt taacagtggt cgtggatatg
    45781 tacctgctga tgaaaataaa aaggataatg caccagttgg aacacttgct gtagattcta
    45841 tttatacacc agttacaaaa gtcaactatc aagtggaacc tgctcgtgta ggtagcaatg
    45901 atggtttcga caaattaacc cttgaaatct tgacaaatgg aacaattatt ccagaagatg
    45961 ctttagggct ttcagcacgt attttgacag aacatcttga tttgtttaca aatcttactg
    46021 agattgctaa gtcaactgaa gtgatgaaag aagctgatac tgaatctgac gaccgtattt
    46081 tagatcgtac gattgaggaa ctggacttgt ctgtgcgttc atacaactgt ttaaaacgtg
    46141 ccggtatcaa tactgtgcat gatttgacag aaaaatctga agcagagatg atgaaagtac
    46201 gaaatcttgg acgcaagagt ttggaagaag tgaaactcaa actcattgat ttgggtcttg
    46261 gattaaaaga taaataaagg aggaatacat ggcttaccgt aaactaggac gcactagctc
    46321 acaacgtaaa gcaatgcttc gcgatttgac aactgacctt ttgatcaacg aatcaatcgt
    46381 gacaactgaa gctcgtgcta aagagatccg taaaactgtt gaaaaaatga ttactctagg
    46441 taaacgtggt gatttgcatg cacgtcgtca agcagctgct ttcgtacgta atgaaatcgc
    46501 atctgaaaac tatgatgaag caactgataa gtacacttct actacagcac ttcaaaaatt
    46561 gttctcagaa atcgcacctc gttatgctga acgtaacggt ggatacactc gtatccttaa
    46621 aactgaacca cgtcgtggtg atgcagcgcc aatggcgatc atcgaattag tataaaatca
    46681 tcaattttgt tgagtgttat gatgatggag tcttgtgctc ttagtctagc tctggtctac
    46741 cgctaggatt tcggtcctag cgggaacact catcataagt tgggatagta gacgcttgtt
    46801 tacgaaattg tttttttctt aagaacaact tcgtaagcag gcgtttttga gtattttcgt
    46861 tagaattatg ctatactatt tgaaaagaat cctgtttaat gttaaggttt cttattttaa
    46921 gaagaattgg agtttactta tgaaagccat tataactgtt gttggtaaag ataaatctgg
    46981 aattgttgca ggtgtttctg gtaaaattgc agaattagga ttgaatattg acgatatctc
    47041 tcaaactgtc ttggatgaat attttacgat gatggctgtt gtatctagtg atgaaaagca
    47101 agattttacc tatcttcgta atgaatttga agcttttggg caaactttga atgtaaaaat
    47161 caatattcag agtgcagcga ttttcgaagc tatgtataat atctaggagg tcatcatgga
    47221 tattagacaa gttactgaaa ccatcgccat gattgaggag caaaacttcg atattagaac
    47281 cattaccatg gggatttctc ttttggactg tatcgatcca gatatcaatc gtgctgcgga
    47341 gaaaatctat caaaaaatta cgacaaaggc ggctaattta gtagctgttg gtgatgaaat
    47401 tgcggctgag ttgggaattc ctatcgttaa taagcgtgta tcggtgacac ctatttctct
    47461 gattggggca gcgacagatg cgacggacta cgtggttctg gcaaaagcgc ttgataaggc
    47521 tgcgaaagag attggtgtgg actttattgg tggtttttct gccttagtac aaaaaggtta
    47581 tcaaaaggga gatgagattc tcatcaattc cattcctcgc gctttggctg agacggataa
    47641 ggtctgctcg tcagtcaata tcggctcaac caagtctggt attaatatga cggctgtggc
    47701 agatatggga cgaattatca aggaaacagc aaatctttca gatatgggag tggccaagtt
    47761 ggttgtattc gctaatgctg ttgaggacaa tccatttatg gagggtgcct ttcatggtgt
    47821 tggggaagca gatgttatca tcaatgtcgg agtttctggt cctggtgttg tgaaacgtgc
    47881 tttggaaaaa gttcgtggac agagctttga tgtagtagcc gaaacagtta agaaaactgc
    47941 ctttaaaatc actcgtatcg gtcaattggt tggtcaaatg gccagtgaga gactgggtgt
    48001 ggagtttggt attgtggact tgagtttggc accaacccct gcggttggag actctgtggc
    48061 acgtgtcctt gaggaaatgg ggctagaaac agttggcacg catggaacga cggctgcctt
    48121 ggccctcttg aacgaccaag ttaaaaaggg tggagtgatg gcctgcaacc aagtcggtgg
    48181 tttatctggt gcctttatcc ctgtttctga ggatgaagga atgattgctg cagtgcaaaa
    48241 tggctctctt aatttagaaa aactagaagc tatgacggct atctgttctg ttggattgga
    48301 tatgattgcc atcccagaag atacgcctgc tgaaaccatt gcggctatga ttgcggatga
    48361 agcagcaatc ggtgttatca acatgaaaac aacagctgtt cgtatcattc ccaaaggaaa
    48421 agaaggcgat atgattgagt ttggtggtct attaggaact gcacccgtta tgaaggttaa
    48481 tggggcttcg tctgtcgact tcatctctcg cggtggacaa atcccagcac caattcatag
    48541 ttttaaaaat taagaaaata ggagaaattt taagttctat ttaagattag atgtgtatac
    48601 tataatcatt aaataaagac ctcctaatat tatttgaaac agataacact gaattagttt
    48661 gaatttgatt ttcatctaat atctttattt aatgaactcc taaacttttt cataataatc
    48721 tccttcaaaa gtcgcctgta tgggtggctt ttattttatc attcatgata taatagaagc
    48781 aaacggagga cggaaaatgg taaaagtacg attgtatttg gtacgtcatg gcaagaccat
    48841 gtttaacacg attggtcgcg cgcaaggttg gagcgatact cccttaactg ctgaaggtga
    48901 acgagggatt caagagttag gaatcggttt gcgagaatct gatctacagt ttgagcgcgc
    48961 ttattcgagt gattctggtc gtaccattca gaccatggga attatacttg aagaacttgg
    49021 cttgcagggg aaaatccctt atcgcatgga caagcgtatc agagaatggt gtttcggtag
    49081 ttttgatgga gcctatgatg gcgatctttt catgggcatt attcctcgta tctttaatgt
    49141 ggaccacgtt caccaattgt cttatgctga actggctgag ggcttggtag aggtcgatac
    49201 agctggttgg gctgaaggtt gggaaaaact cagtggccga atcaaggaag gctttgaaat
    49261 aattgcaaaa gaaatggaag atcaaggtgg aggtaacgcc cttgttgtca gccatggaat
    49321 gactattgga accattgttt atctgattaa tggcatgcat ccgcatggtc tagataatgg
    49381 tagcgtgacg attcttgaat atgaggacgg tcagtttagg gttgaagttg tcggtgaccg
    49441 tagttaccga gagctaggac gtgagaagat ggaagaaggc tctatttaat cagtctagac
    49501 ttgcttgcca tgagctaggg atttgataag aatatcaaga taagaaaaaa cagccgaggg
    49561 tactcctttc ggctgttttt gatgtggaaa actaaagtgt aatgctattg cttttagaga
    49621 ttttcataaa caagagcaag gaacctactg ttagaacagt caggatagtt gacaaggctg
    49681 cggctacacc gtaatttcct ctgagaacct ctgtataaat agctacagtc attgttcttg
    49741 ttttgacatt gtagaggagg atagaggata gaagtagaga gttctgaaat cagtgtgacc
    49801 caagatagga tggctccaga aataataccc gatagcatca ttggagttgt aatcttagca
    49861 aaggtattga gacgactact tcctaagctt tcagcagctt cttcaatact tggtgctatt
    49921 tgttgtaagc tagcaacaga tgagcgaata gtatacggta atcttctgac agatagagac
    49981 ataatcaaga tgaaagcagt ccctgtaatc ataagaaatc cacttccaaa tagaccagta
    50041 ttgaaggaag aaatgaaggc aatccctaga acggttcctg gtacaatata aggtaccata
    50101 ctgaggctgt caattaagtt tgtaaacaaa ttccgttttc taacggctag gtaggagata
    50161 aatgtcgcaa atagaacaac tagaactaag gcaatcaaag ggatacgaat ggtattgaaa
    50221 atagcagatc ccatacgatt gaaagctagc ttgtaactgt ttggagaata acctttaaaa
    50281 aataccatac ctgatgtttt taggaaagag gtataaatta agtagatttg aggtaaaaca
    50341 gagataaaga taattccgta gactgttgca taaatggcag ccatttttcc ttttgtagtt
    50401 tttttaggct caattggatg gagcagattc atgctgaaac tgtagcggtt tgcaatgtgt
    50461 ttttggataa ggaaaattgc caaggcaatg ataatcgcca taattgcaaa agcagaattt
    50521 cctccaacct cgctaataaa ttgggtataa atcaggacag ggaaagtccg atatccttcg
    50581 ccaatcaaca taggcgttcc aaagtctgag aatgctctca taaatacaag caaggagctg
    50641 ctagtaaggt tggaactaga agaggtaaaa caaccgttac gataggttta aatccgaagg
    50701 accccatgct ttcagctgct tcaagtagag aattgtcaat actgttcatt gttccagcaa
    50761 catatagaaa taccagtggg aatagttgca gtgtaaagac aagtacaatt cctttgaatc
    50821 cataaatatc gatagctgga agatgaaggg catttgtcaa aaatttagtg atgaccccat
    50881 ttcgtcccag caagagaacc caggagtagg ctcctacgaa aggagctgac atggaagcaa
    50941 tgataatcaa tatttgtaga aatttcttcc ccttgaagtc atacatagag aagagataag
    51001 ctaatagggt tcctacaact aaggaagtga cagtagcggt aatggaaacc ctgaaactgt
    51061 tgactagtgt ctcagagtag taggctttac taaagaaagt gacaaaatta gctagtgaga
    51121 attgtccttc atgtataagt gcttgcttga gcacggtaat gataggataa acgagaaaga
    51181 caagataggt aagaaagagg aagaaagagg aggctgtcca aatatttagt tttttacgtt
    51241 ccatggttga ctccttttat caggttttgg gaaccatctg cagaaaagat gtttaatttt
    51301 tgcgtattga ttcgtagacg aatacgattg cctttttgta gttcttcttc aaaagttgat
    51361 tcttcactaa cttgaatttt tgaggcaaaa cctgtctcaa tgaaatactc cgtatttagt
    51421 ccaagataga cgctatcgct aatagttcct tcaatatctc cagattcatc tttgataaac
    51481 tcttcgggac gaatgcttac atgaatagct tgctcctcaa cctgatcaag agctggcatt
    51541 cgaagggcat agccatctga aaagacgata taagcgccgt cgctccgttt ttcaagattg
    51601 gcagggataa tatttgtgcg tccgataaag gttgccacaa actcattagc tggtttatga
    51661 tagagttctt ttggtcggcc gatttgttgg atcaccccat ctttcataac agcaatttga
    51721 tctgaaatag ccatggcttc ttcttggtcg tgggttacat aaacagttgt aattcccact
    51781 tcgtgttgga tttctcggat ggcttgacgc atatccaagc gaagtttggc ctccagatta
    51841 ctaagtggct cgtccatgag gagaacactt ggattaaccg ctaaggcgca tgccaaggtg
    51901 acacgttgtt gttgtccacc actgagttta tcgggctttc gatccgcata ttgagcaatt
    51961 tgcatgagtt caagatactt gttggtctgt tgaatcaatt cttcttttgg aaccttcttt
    52021 tgcttaagac caaaagcaac gttgtctcgg acagtcaaat gtgggaaaat agcgtagttt
    52081 tggaaaacca tcccgatatt gcgtttgctg ggttccatat tattgatttt tgtatcatcg
    52141 aagtaaaatt ctccatcttt gatactgttg aaacctgcaa tcatacgaag aagggtcgtt
    52201 ttcccacatc ctgaaggtcc aagaagggta aagagacttc cttttggaat tgtaatgttc
    52261 aaattctcaa taacagggac atcgtggtag attttttggc gttaataatt ttgatctcac
    52321 tcatagtgaa ccttttttac tgtttagatt ggatatctgt aaagacttcg ttgtatttct
    52381 taacgatatc tgatttattc ttgatgacat aatcataatc ttcagtgagt gttttgattt
    52441 tgtcaattgg tttcatgttt tcgcttgttt tagcattttt acgaacagga cggttagtag
    52501 tggttgtacc aagtgtatct tgtacttctt gagagataat aaaatcgata aatttcttgt
    52561 cattttccat atttttagct tttttaacga tagcagcact agcaggtagg aagacggttc
    52621 cttcttttgg atagactact ttaatgttag ctccgtcatt taagagttta actgctggat
    52681 cctcataaga gagaccaaca gccatttctc catcaacgac tgctttatag acaccagatg
    52741 aacttgaacc gattttatag tgtattgaaa ctagaatagt acacctctac ttctaaaaca
    52801 ttgttagaaa tcgatttgac tgtcctgatt aatttgtcct gttcttattt catttcacta
    52861 tgccatcaat aagtgtgaaa agatctttta cataagacca agccttatca tctttgtaac
    52921 caccttgagc ttgtagcata tttgttaatt gagcaaaggc gctagaagag tttgctgggt
    52981 cagcagttgc gattttttct tttagttcag gtttgagaag atcgttatat ccttcgatgt
    53041 tcatgccttt agttaaatca gggtggacga ttaaaacact accatctagt gtataaggag
    53101 tagagaagcc agttgtgttt tgatattctt tgataacatt atcattttct tttgaagtat
    53161 agttttcaaa gagttctccg tgggtagtat attgtgtata agaaccacca aagataacat
    53221 cagctacagg aacttctttt tctgactcta gttttttgaa aagttctcca gtaccagctt
    53281 gaatcagttc tactttgata ccatattttt cttcaaaggc aggaatagtt gctccaatta
    53341 agccctctga gtttggtgaa taaacgacta gcgaaccgcc gtctccttta tcagatgaac
    53401 tgtcatcggc agattcatta gaagaacaag cagcataata catccatttc tttttcatga
    53461 tggatacctc cgttgtgtta tttaagttta ttttaaaaca atgtaagcgt ttttaaaaca
    53521 tacaattcta ttctatagtg tattgaatct ataacagtac actttgactg ctaaaatatt
    53581 tctataaatt aatttgactt tcctgataga gatgttcaca tcttatttca attcactata
    53641 ttagagtaaa attctctaca aaaagaagaa tagcctattt tactattctt ctgagtgatt
    53701 tcaattcctt tggggaaata tggagatact ttttaaatcc tgacaaatgg ttgtttcttt
    53761 ttctaaatcg gtgatactgt atcggagaat gcgcgtgagg tcacaaaggc tgcgatagag
    53821 cttctatgga gaatttcttt ttggagagat tttttaaagg aatgagacat ccgctacctc
    53881 cttggaaggt ttttttgtga taagtatagc agtttaaaaa ttctagagca aggtaaaata
    53941 gaaaaggttt ggctaaaatt ttttagctaa accttttgaa aagttaagta tgtattatag
    54001 atgcttgtta tctgcaaata gctagagcat ttcaaggaaa taaactagaa ataacaatta
    54061 atatggtggt tcagaaatac cttttgataa tcaataagat cttcaggatg aagtgcgttg
    54121 attccatcca tttcatattt ccgatttaat aaacgatatt tgttttcacc aaattgatga
    54181 aaagggagta gttgaacttg gtcgatattt aatgagttaa atagagtagc gaatttttct
    54241 gcatcctcta aactattgtt aaaattagga ataactggga ttcttaaaac gatagtttta
    54301 ttttgtgaaa aagcataatg aatgttttta ataatcattt gattaaaaac cccagtcact
    54361 tttttatgtt ttatagaatt ataatgtttt aggtctgtgt agataaaatc cacatattga
    54421 attaaatcaa taaatttttc atgatcaaca aaggcagtag tttcaatggc agtgtgtatg
    54481 tgatgttctt tagctgattt taagatggct ttagcaaatt caaactgagc aaatatttca
    54541 cctcctgata aagttaaacc tccaccggat tcttcgtaaa attctttgtc ttttaatacc
    54601 tctgtaataa tttcttctac agtcttttct tcaccgacta aggtgaattt ctctcgttga
    54661 gcatctttca ttttttcagg tttcattctt tgagattcag gattagaaca ccatggacag
    54721 cgcagaggac atccttttaa aaaaacagtt gtacgaatac ccgggccgtc atgaattgaa
    54781 aagtgttgaa tattaaaaat aattccttta gatatttcca taagagttcc tcctcttcat
    54841 aagtctatcc tatcacaaaa acgaaagaaa aacaattacg aacgaaaggt taatttattg
    54901 ttttttgctt tttattttgg tagaataaaa cggaggtgta aaatgaaaag attggaacaa
    54961 attattaaat tagtatcaga atatgaaaag atcgacgtta atacattatc ggaaaaatta
    55021 aatgtatcga aagtaacgat tagaaaagat ttagataaat tagagtcaaa aggtttatta
    55081 cacagagagc atggatatgc tgtattaaat agtggagatg acttaaatgt acgtttgtca
    55141 attaattatg aaattaagag aaaaattgtt caggaagcag taaaattggt gtcagataat
    55201 gaaacaataa tgatagaatc tggatcgacc tgtgctttac ttgctgagga aatttgcaag
    55261 caaaaaagaa atgttacgat tgtaacaaat tcatttttta tagcaaattt tgtgagagct
    55321 tatgattcat gtcgtattat tgttcttggt ggagagtttc agaaggattc acaggttact
    55381 gtgggacctt tattaaaaga aatgatacag acttttcatg tgcatcaagc ttttgttggg
    55441 acagatggtt acgataaaga gatgggcttt acaggaaaag atttaatgcg cagtgaggta
    55501 gttcaatata tttcagcagc gtcggataaa gtcattgtac taactgactc aagtaaattt
    55561 gataaaaggg gtacagtaag aagatttgct ttaagtcaag tctatgaagt aataacagac
    55621 gaaaaacttt ctaaacaaaa tatagctaca ttagaaaatg ctgggataat ggttaaggta
    55681 gtttcgtaag aggttaagtg tatgaatcaa gataggaata aactgctctc taaaattgct
    55741 tatctgtatt atattgaaaa cttaaatcag tcacaaatag cagcaaaatt aggaatttat
    55801 agaacctcta ttagtagaat gttaacagaa gcaaggaatg caggaattgt taaaattgaa
    55861 atagagaatt ttgataccaa tatgtttaag ttggaaaatt atgtaaaaga aaaatatggt
    55921 ctggaaagtt tagaaattat tccaaatgaa tttgatgata atccaacaat tttatctgaa
    55981 agaatttctc aagttgcagc aggtgtcctt aggaatctaa ttgatgataa tatgaaaatt
    56041 ggcttttctt gggggaaaag tttaagtaat ttagtagatt taattcacag taaaagtgtc
    56101 cgaaatgttc acttctatcc tctagcaggt ggtcctagtc acatacacgc taaataccat
    56161 gtgaatacac tgatttatga aatgtctaga aaatttcatg gagagtgtac atttatgaat
    56221 gcaacgattg tgcaagaaaa taaattgtta gcagatggta ttttgcaatc aagatatttt
    56281 gaaaatttga aaaatagttg gaaagattta gatatagctg tagtcggaat tggtgatttt
    56341 agcaataaag gaaagcatca atggttagac atgcttacag aggatgactt taaagaattg
    56401 accaaagtta aaactgtagg agaaatttgt tgtcgatttt ttgattcaaa aggtaaagaa
    56461 gtctatgaaa acttacagga aagaacgata gcaatctctt tggaagattt aaaaaatatt
    56521 cctcaaagtt tagctgttgc ttacggtgat acgaaagtat cttcgattct ttctgtcttg
    56581 cgtgctaatt tagtaaatca tttgattaca gacaaaaata caattttaaa agttttggaa
    56641 gaagatgggg atttgacttt tagagagatt ctaggtgagt gaaaatgata gactgattca
    56701 gtttatcatt tttcttttta gttgattgca catttgtgct tatataaaca aaaatagttt
    56761 atctgttgtt tttggattga caactttatt atgtagttgt attctatagt tacaaaagaa
    56821 aattttaaaa tttcaaatga aaaaagcttt ttacatagtg aaatgaggag gaatttatgg
    56881 aaattattgt tccagatcaa attatcatgg gtttaatttt atatgctggc gatgcgaaac
    56941 aacatattta taaagcatta gattacataa agaacggtac atgtgaacga tgtgaagaag
    57001 aaatccagtt agctgatgca gccttattag aagctcataa tctacaaaca aaattcttgg
    57061 cacaggaagc gtctggtaca aagacagaaa ttacagctct ctttgttcat tcacaagatc
    57121 atctcatgac tagtatgacg gagattaatt taatcaaaga aattattagt ttgagaaaag
    57181 aacttcataa aaaataatac tagagtatta tcattgttat taacatagag gaggaaaaca
    57241 taatggtgaa gattggtttg ttttgtgcag caggtttttc tactggtatg cttgtaaata
    57301 atatgaaaat tgcagcgcaa tctagtggag ttgaggcaga aatagaggcg ttttctcagt
    57361 ctaaattagc ggattatgcg ccaaatatag atgttgcact attgggtcca caagttgctt
    57421 atacattaga taaatcaaaa gaaatttgtg ataagtgtga tgttccgata gctgttattc
    57481 cgatgatgga ctatggtatg ttagatggga aaaaagtatt agatttggcc ctatctttga
    57541 ttagtgggta agaaaaggag atttattatg tcaaagatgg atgttcagaa aatcattgca
    57601 ccgatgatga agtttgtgaa tatgcgtggc attatagctc taaaagatgg gatgttagca
    57661 attttgccat tgacagtagt tggtagtttg ttcttgatta tgggacaatt gccgttcgaa
    57721 ggattaaata agagcattgc tagtgttttt ggagctaatt ggacagagcc gtttatgcaa
    57781 gtatattcag gaacttttgc tattatgggt ctaatttctt gtttttcaat tgcctattct
    57841 tatgctaaga atagcggagt agaggcttta ccagctggag ttctatctgt atctgcattc
    57901 tttattttgc taagatcatc ttatatccct aaacaaggtg aggcgattgg ggacgctatt
    57961 agtaaagttt ggtttggagg ccaaggaatt atcggtgcta tcattatagg tttggtagta
    58021 ggaagtattt ataccttctt tataaagaga aaaattgtta ttaagatgcc agaacaagtt
    58081 ccacaagcta ttgcgaaaca gtttgaagca atgattccag catttgtaat tttcttatct
    58141 tctatgattg tatatatttt agcgaagtca ttgactaatg gcggaacatt catagaaatg
    58201 atttattctg ctattcaagt tccgttgcaa ggtttaactg gatctttgta tggtgctatt
    58261 ggaattgcat tctttatatc atttttgtgg tggtttggtg ttcatgggca atcggtagta
    58321 aatggagtag tgacagctct gcttttatct aatcttgatg ctaataaagc tatgttagcc
    58381 tctggtaatc tatcattaga aaatggtgca catattgtta ctcaacaatt tttagattca
    58441 tttttaattc tatcaggttc agggattacg tttggtcttg tagttgccat gctttttgca
    58501 gcaaaatcaa aacaatacca agccttagga aaagttgcag cttttccagc aatatttaac
    58561 gtaaatgagc cagttgtatt tggatttccg attgtcatga atccagttat gtttgtacct
    58621 ttcattcttg ttcctgtact tgcagctgtg atagtatatg gagctattgc aacaggtttc
    58681 atgcagccat tctcaggggt aacattgcct tggagtacac cagctattct atcaggattt
    58741 ttggtgggtg gatggcaagg tgttgtgact cagttattag tattggtgat atctacagtg
    58801 gtttactttc cattctttaa gatacaagat cgtttagctt accaaaatga aatcaaacaa
    58861 tcttagaggt atttgtgtgt tactgttaaa ctcacacatt tgtgctaaaa attagagagt
    58921 taaaattttt ctagttaaaa gcttgaaaat ttctataaaa atcggtatta tattttcgaa
    58981 agaaataaaa atattttcga aagaaaggtg cttacgatgg taaatacaga agtagcaaga
    59041 acaacaatca agacagaata ttttggcagc cttactgaaa ggatgaacaa atatcgagaa
    59101 gatgttttaa ataaaaaacc ttatattgat gctgagagag cagttctagc aacacgcgcc
    59161 tatgaacgat acaaggaaca acctaatgtc ctaaaacgtg catatatgct gaaagaaatt
    59221 ttggaaaata tgtctatcta tattgaagaa gaatctatga ttgcgggaaa tcaagcttct
    59281 tccaataaag atgctcctat ttttccggaa tatacgctag aatttgttct caatgagttg
    59341 gatctttttg aaaagcgtga tggagatgtt ttctatatta cagaagaaac aaaagaacaa
    59401 cttagaagta ttgctccgtt ttgggaaaat aataatttac gtgctagagc tggtgcctta
    59461 ttacctgaag aagtgtctgt ttatatggaa acaggattct tcggtatgga aggtaagatg
    59521 aattctggag atgctcactt agcagttaac tatcagaaac ttttgcaatt tggtttaaga
    59581 ggttttgaag agcgggctcg taaagcaaaa gtagctctag atttaacaga tccagcaagt
    59641 attgataaat atcattttta cgactctata tttatcgtaa tcgatgctat taaagtatat
    59701 gcaaagcgct ttgttgctct tgctaaaagt ttagccgaaa atgcaaatcc taaacgtaag
    59761 aaagaattac ttgagattgc agatatttgc tctagagtcc catatgaacc ggcaactact
    59821 tttgcagaag ctattcaatc agtttggttt attcaatgta ttttacaaat tgaatctaat
    59881 ggccactctc tttcatatgg ccgttttgat caatatatgt atccatatat gaaggctgat
    59941 ttagaaagtg gtaaagaaac agaagatagc attgttgaac gtctgacaaa tctttggatt
    60001 aagacaatta caattaataa ggttcgcagt caatcacata cattttcttc agcaggaagt
    60061 cctttatatc aaaatgttac aattggtgga cagactcgag ataagaagga tgctgttaac
    60121 ccattatctt atttggtatt aaaatcagtt gcacaaaccc atctaccgca acctaatcta
    60181 actgtacgtt accatgcagg tttagatgct cgtttcatga atgagtgtat tgaagtgatg
    60241 aaacttggtt ttggtatgcc tgcatttaat aatgatgaga ttattattcc ttcttttatt
    60301 gcaaaaggag tattggaaga tgatgcttat gattacagtg ccattggatg tgttgaaacg
    60361 gcagttccag ggaaatgggg ctatcgttgc acaggtatga gttatatgaa cttccctaag
    60421 gttctactta tcacgatgaa tgatggaatt gatccggctt cgggtaaacg gtttgcacca
    60481 agctttggtc attttaagga tatgaagaac ttttctgaat tagaaaatgc ttgggataaa
    60541 acactaagat atttgacacg aatgagtgtt attgttgaaa attctattga tttatcattg
    60601 gaacgagaag ttcctgatat tctatgttca gcattgactg atgattgtat tggtcgtgga
    60661 aaacacctta aagaaggtgg agcagtatat gattatatat caggattgca agttggaatt
    60721 gcaaatttgt cggattcgtt agctgcaatt aaaaaattgg tgtttgagga agaacgtata
    60781 agcccaagtc aactttggca tgcactggaa acagattatg ccggagaaga aggtaaggtc
    60841 attcaagaaa tgttgattca tgatgcacct aagtatggta atgatgatga ttatgctgac
    60901 aaattggtta ctgctgctta tgacatttat gttgatgaaa ttgctaaata tcctaataca
    60961 cgttatggaa gagggcctat tggaggaatt cgttattcag gaacatcttc tatatcagcc
    61021 aacgtagggc agggacgtgg aacattagca actccagatg gacgcaacgc gggtacaccg
    61081 ttagcagagg gttgttcacc atcacataat atggatcaac acggccctac atctgtttta
    61141 aaatctgttt caaaattacc aacagatgaa atcgtaggtg gggttctctt aaatcagaaa
    61201 gtaaatcctc aaacgttagc caaagaagaa gataaattaa aactaattgc tttgttacga
    61261 acattcttta atcgtttaca tgggtaccat attcaataca atgttgtttc cagagagacg
    61321 ttgattgacg ctcagaaaca tcctgaaaaa cacagagact taattgttcg tgttgcagga
    61381 tactctgcat tcttcaatgt tctttctaag gcaacccaag atgacattat aggacgtact
    61441 gagcatactt tgtaaaataa agaggttctt tttatggaat ttatgcttga cacattaaat
    61501 ttagatgaga ttaaaaagtg gtctgaaatt ttgccgctag ctggggtaac ttcaaatccc
    61561 actattgcaa aaagagaggg ttctattaat ttttttgaac gaatcaaaga tgtaagagaa
    61621 ttgattggct ctacaccctc tattcatgtt caggtgattt ctcaagattt tgaaggcatc
    61681 ttaaaggatg ctcatgaaat tcgaagacaa gcaggagatg atatatttat caaagtacct
    61741 gttactccag ctggattacg tgcaataaag gtgctaaaaa aagagggcta ccatatcact
    61801 gcaacagcta tttatacagt tattcaggga ttattagcta tcgaagtagg agcggattac
    61861 ctagctccat attataatag aatggaaaat ctgaacattg attcaaattc tgtcattcgt
    61921 caattagctc ttgctattga tagacagaac tctcctagta agattttagc tgcatccttt
    61981 aaaaatgtag cacaagtaaa taatgcttta gctgcaggtg cgcatgctgt tacagcagga
    62041 gcggatgttt ttgaatcagc tttcgccatg ccatctatcc aaaaggcggt tgatgatttt
    62101 tctgacgatt ggtttgttat tcaaaatagt cgttccattt agatagagag gaaatacata
    62161 tgagaatttt tgctagtcct tctagatata ttcaggggga aaatgccttg tttgaaaatg
    62221 ccaaatcaat tttggatttg ggaaattgcc ctattctatt atgcgatcag ttggtttatg
    62281 atattgttgg aaaacgattt gaagattacc tacataggta tggtttccat attgttctgg
    62341 cgctatttaa tggtgaagct tctgacaatg aaatcaatcg agttgttgcc ttggctgaga
    62401 aagaaaattg tgatagtatt atcggtcttg gtgggggaaa gacgattgat agcgcaaaag
    62461 ctattgcaga tttgattgaa aagcctgtta ttattgctcc aacaattgca tcgaccgacg
    62521 cacctgtatc tgctttatct gttatttata cagatgaagg tgcatttgat cattatctat
    62581 tttattctaa aaatccagat ttagttttgg ttgatacaaa agttatttca caagccccta
    62641 agcgtttatt agcgtctggt attgcagatg gtttagcaac ttgggttgag gcgcgtgcgg
    62701 ttatgcaggc aaatggaaaa actatgttgg gacaacagca aacattggct ggagttgcaa
    62761 ttgcgaagaa atgtgaagaa acgctgtttg cagatggttt acaggctatg gcagcttgtg
    62821 aagctaaagt ggtgacacca gcattagaaa atattgttga agctaatact ttattgagtg
    62881 gtctaggttt tgaaagtgga ggattagctg cggcgcatgc aattcataat ggttttactg
    62941 cattgacagg tgacattcat catttaacac atggtgaaaa agtagcttat ggaactttag
    63001 tacaactatt attggaaaat agacctaaag aagaacttga taagtatatt gagttttaca
    63061 aaaaaattgg tatgccaaca actctaaaag aaatgcattt ggatcaagtt ggatatgatg
    63121 atttaataaa agttggtaaa caagcaacta tggagggtga gacaattcat cagatgccgt
    63181 ttaagatttc gccttcagat gttgctcaag ctattatcgc tgtagatgcc tatgtaaatt
    63241 caaaataaac aataaggact actgttttcc aaatggtagt cttttattga tccctgtatt
    63301 gaattctata gaagattgaa ataggatgag aacaaatcga ttgggaaagt aaaattaatt
    63361 tctataaatg ttttagcaat tgtttcgtac tatttcagat tcagtctact atatgttctt
    63421 cataaatcaa aaagcgacat aggttgtcgg ctatttattg tgaatacatt aattagcatt
    63481 ccagttttat cttcggtcta aaataagtat tttgtgctat acgagataag cttcttgact
    63541 tactccttga tttactgcat aacaatggga taaaaagtgg gagatagagc aattcatagt
    63601 catcaaaatt aatgagatac agtatacagt ttttccttta aacacatttc aaattccctc
    63661 aaaaatggta taatagtaac atcacaaaat tggagagaga ccatgagttt ttacaatcat
    63721 aaagaaattg agcctaagtg gcagggctac tgggcagaac atcatacatt taagacagga
    63781 acagatgcat caaaacctaa gttttatgcg cttgatatgt tcccttatcc gtctggagct
    63841 ggtctgcacg taggacaccc agaaggttat actgcaaccg atatcctcag tcgttacaaa
    63901 cgtgcgcaag gctacaatgt ccttcaccca atgggttggg atgcttttgg tttgcctgca
    63961 gagcaatacg ctatggatac tggtaatgac ccagcagaat ttacagcgga aaacattgcc
    64021 aacttcaaac gtcaaattaa tgcgcttgga ttttcttatg actgggatcg tgaagtcaac
    64081 acaacagatc caaactacta caagtggact caatggattt tcaccaagct ttacgaaaaa
    64141 ggcttggcct atgaagctga agtgccagta aactgggttg aggaattggg aactgccatt
    64201 gccaatgaag aagtgcttcc tgacggaact tctgagcgtg gaggctatcc agttgtccgc
    64261 aaaccaatgc gccaatggat gctcaaaatc acggcttacg cagagcgctt gctcaatgac
    64321 ttagatgaac tagattggtc agagtctatc aaggatatgc aacgcaactg gattggtaaa
    64381 tcaactggtg ccaatgtaac tttcaaagta aaaggaacag acaaggaatt tacagtcttt
    64441 actactcgtc cggacacact tttcggtgcg actttcactg tcttggctcc tgaacatgaa
    64501 ttagtagacg ctatcacaag ttcagagcaa gcagaagctg tagcagacta taaacaccaa
    64561 gccagcctta agtctgactt ggctcgtaca gaccttgcta aagaaaaaac aggggtttgg
    64621 actggtgctt atgccatcaa ccctgtcaat ggtaaggaaa tgccaatctg gattgcagac
    64681 tatgtccttg ctagttatgg aacaggtgcg gttatggctg tgcctgccca cgaccaacgt
    64741 gactgggaat ttgccaaaca atttgacctt ccaatcgtcg aagtacttga aggtggaaat
    64801 gtcgaagaag ctgcctacac agaggatggc ctgcatgtca attcagactt cctagatgga
    64861 ctcaacaaag aagacgctat tgccaagatt gtggcttggt tggaagaaaa aggctgtggt
    64921 caggagaagg ttacctaccg tctccgcgac tggctcttta gccgtcaacg ttactggggt
    64981 gagccaattc caatcattca ttgggaagat ggaacttcaa cagctgttcc tgaaactgaa
    65041 ttgccgcttg tcttgcctgt aaccaaggat atccgtcctt caggtactgg tgaaagtcca
    65101 ctagctaact tgacagattg gcttgaagtg actcgtgaag atggtgtcaa aggtcgtcgt
    65161 gaaaccaaca ctatgccaca atgggctggt tcaagctggt actacctccg ctatattgat
    65221 ccacacaata ctgagaagtt ggctgatgag gacctcctca aacaatggtt gccagtagat
    65281 atctacgtgg gtggtgcaga gcatgctgtt cttcacttgc tttacgctcg tttctggcac
    65341 aaattcctct atgacctcga tgttgttccg actaaggaac cattccaaaa actctttaac
    65401 caagggatga ttttgggaac aagctaccgt gaccaccgtg gtgctcttgt ggcaaccgac
    65461 aaggttgaaa aacgtgatgg ttccttcttc catgtagaaa caggggaaga gttggagcaa
    65521 gcgccagcca agatgtctaa atcgctcaag aacgttgtta acccagacga tgtggtggaa
    65581 caatacggtg ccgataccct tcgtgtttat gaaatgttta tgggaccact cgatgcttcg
    65641 attgcttggt cagaagaagg tttggaagga agccgtaagt tccttgaccg agtttaccgt
    65701 ttgattacaa gtaaagaaat ccttgcggaa aacaatggtg ctcttgacaa ggtttacaac
    65761 gaaacagtca aagctgttac tgagcaaatt gagtctctca aattcaacac agctattgcc
    65821 caacttatgg tctttgtcaa tgctgctaac aaggaagata agctttatgt tgactatgcc
    65881 aaaggcttta ttcaattgat tgcaccattt gcacctcact tggcagaaga actctggcaa
    65941 acagtcgcag aaacaggtga gtcaatctct tatgtagctt ggccaacttg ggacgaaagc
    66001 aaattggttg aagatgaaat tgaaattgtc gtccaaatca aaggaaaagt tcgtgccaaa
    66061 ctcatggttg ctaaagatct atcacgtgaa gaattacaag aaatcgcttt agctgatgaa
    66121 aaagtcaaag cagaaattga cggtaaggaa atcgtgaaag taattgcggt accgaataaa
    66181 ctcgttaata tcgtcgttaa ataacgagtt tattagctct atctgccacc ttcaatagtc
    66241 cactggacta ttgaagccaa ctaaattagt taacattgtt gtgaaataaa aagaagtaac
    66301 tctagagtat gaactttaga gttactttta gaataggtca gaatgaatag attagtgaac
    66361 gatagcagga agtgtgaaat tttattattt aagatttcta ttattgtagt gtcagttata
    66421 gtattagtaa tgtcaatagt gggtgcatat tattttaaaa attgtctttg ggacaaggaa
    66481 agtttaaatt tgacactcac tttagacact ctctttgcag tttttattac tttctttagt
    66541 ttttcaaatg ataaatcaag caatattttt ggaatatctc caaatgaaat tataaggaca
    66601 cagtctgaag tgtatcaatt tttatttaca aaatcttttg caactctatt aatagtgaca
    66661 aatttgttat ccattattca ttattttgga gccaaaaatt taatagtttc ttttaatgaa
    66721 acttatcctc gtattcaatc cagtgttggt atcatgtggc taccactgtt tggtattctg
    66781 ataatctatt ttatttggct cttaatagag ggattaaaat atatcagaaa aataaattat
    66841 attattcaaa atagggaaat aatctatttt tattcgcagg taaaagttgt agataaactt
    66901 actgattctg aatcgaaaaa atacggtgaa tatattgatt tgtacgaatc gtgtcgtcag
    66961 aacaatttaa gtgttctact taactttctt caaaattcgg atgataaacg aggagttatt
    67021 gctagtaact cagtgagaat tatgtttgaa tctttggata aagcagacca aattgcggcc
    67081 ctgaatattt gtagaaacta tgtagcacga gaaaatgttt taaataaaga agatgcagaa
    67141 aaattgttaa atgaaattga taaaaaactt gaaattagca gataggacaa ttcaaataga
    67201 gttgagtaaa aagtttgaaa agtgaaacaa ggagaaagct atgccagtaa atgaatatgg
    67261 tcagatgatt ggtgagtcaa tggaaggtta tacacccggt gcactgcctt ctattgattt
    67321 cttagaaggg cgttatgcta ggatagaggc tctctcggta gaaaaacatg cggaggattt
    67381 gctagctgtt tatggcccgg atactcctcg ggagatgtgg acctatcttt ttcaggagcc
    67441 agtagcagat atgggggagc tggttagcct tttaaatcag atgttggctc gtaaggaccg
    67501 tttttactat gcaatcatag acaaggcgac tggtaaggct ttgggaactt tttctctcat
    67561 gcgcattgac cagaataacc gagtaataga agtcggagca gtcacttttt ctccagagct
    67621 aaggggtaca cggataggaa ctgaggctca gtatctccta gctcgctata tttttgagga
    67681 gctaaactat cgtcgctacg agtggaaatg cgatgcctta aatctgccat ccagacgagt
    67741 tgcggagcgt ttgggatttg tctatgaagg aaccttccgc caggcggtgg tttataaggg
    67801 gcgtacgaga gatacggatt ggttgtctat gattgatatg gactggcctc aagtcaaaga
    67861 tcgtttggaa acatggctgc gtcctgaaaa ttttgataaa aatggacagc agtacaagag
    67921 cttgagagaa ctttaagagg tgttgagatg attactatta aaaagcaaga aattgtcaag
    67981 ctagaggatg ttttgcatct ctatcaggct gtcggttgga caaattatac ccatcaacca
    68041 gagatgctgg agcatgcctt atcgcattca ttagtaattt atctggcact tgatggtgat
    68101 gctgtggtgg gcttgattcg tttggttgga gatggattct catcagtttt tgtacaggat
    68161 ttgattgttt taccaatcta tcagcgtcaa gggattggta gtgccctaat gaaagaggcc
    68221 ttaaaagatt acaaagatgc ttatcaagtc cagctagtga cagaagagac agaaaaaaac
    68281 gtgggatttt atcgttctat gggctttgaa atcttatcca cctataattg tatagggatg
    68341 acttgggcga atcgagaaaa ataacaaaac ttgttttttc ttaagcaaaa tttaaggatg
    68401 gtctagtatt atataat
//
LOCUS       DBEROU010000005        60213 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.005, whole genome shotgun sequence.
ACCESSION   DBEROU010000005 DBEROU010000000
VERSION     DBEROU010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 60213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60213
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.005"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..263)
                     /locus_tag="ACL8CL_000347"
     CDS             complement(<1..263)
                     /locus_tag="ACL8CL_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGS1329658.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRKYDKEPYE"
     gene            complement(385..2343)
                     /gene="ftsH"
                     /locus_tag="ACL8CL_000348"
     CDS             complement(385..2343)
                     /gene="ftsH"
                     /locus_tag="ACL8CL_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HGS1329659.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
     gene            complement(2359..2901)
                     /gene="hpt"
                     /locus_tag="ACL8CL_000349"
     CDS             complement(2359..2901)
                     /gene="hpt"
                     /locus_tag="ACL8CL_000349"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HGS1329660.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            complement(2905..4182)
                     /gene="tilS"
                     /locus_tag="ACL8CL_000350"
     CDS             complement(2905..4182)
                     /gene="tilS"
                     /locus_tag="ACL8CL_000350"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HGS1329661.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRDAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEKEDELVLHYQNQVAYQGYLFSFGLPLEG
                     ELIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            complement(4179..5447)
                     /locus_tag="ACL8CL_000351"
     CDS             complement(4179..5447)
                     /locus_tag="ACL8CL_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HGS1329662.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQY
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            complement(5440..5562)
                     /locus_tag="ACL8CL_000352"
     CDS             complement(5440..5562)
                     /locus_tag="ACL8CL_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0009 family protein"
                     /protein_id="HGS1329663.1"
                     /translation="MENLLDVIEQFLSLSDEKLEELADKNQLLRLQEEKERKNA"
     gene            complement(5567..5935)
                     /locus_tag="ACL8CL_000353"
     CDS             complement(5567..5935)
                     /locus_tag="ACL8CL_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HGS1329664.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            complement(5928..6194)
                     /locus_tag="ACL8CL_000354"
     CDS             complement(5928..6194)
                     /locus_tag="ACL8CL_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HGS1329665.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDAAGMYEIISETRVEENV"
     gene            complement(6252..9761)
                     /gene="mfd"
                     /locus_tag="ACL8CL_000355"
     CDS             complement(6252..9761)
                     /gene="mfd"
                     /locus_tag="ACL8CL_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HGS1329666.1"
                     /translation="MVTLLDLFSENDQIKKWHQNLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEISQLEPCRIEFFGDEIDGIRSFEVETQLSKENKT
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERDLAQYFTEELQNSKAFSDM
                     QYFSDIEQIYKKQSPVTFFSNLQKGLGNLKFDKIYQFNQYPMQEFFNQFSFLKEEIER
                     YKKMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVNLIEGNLRHGFHF
                     VDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVVHHIHGIGQYLGI
                     ETIEIKEIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKLNKLNDGHFKKAK
                     QKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYVETDDQLRSIEEI
                     KRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPTTVLAQQHYTNFK
                     ERFQNFAVNVDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSKDVVFADLGLMII
                     DEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLSVIETPPTNRYPV
                     QTYVLEKNDSVIRDAVLREMERGGQGYYLYNKVDTIVQKVSELQELIPEASIGYVHGR
                     MSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADHMGLSTLYQLRGR
                     VGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMRDLSIRGAGNLLG
                     KSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRIKGNAELILQIDAYLPDTYISDQ
                     RHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLVKSYLDKVFVQRV
                     ERKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENKGLMELVFDVQNKKDYEI
                     LEGLLIFGESLLEIKESKEENSI"
     gene            complement(9762..10331)
                     /gene="pth"
                     /locus_tag="ACL8CL_000356"
     CDS             complement(9762..10331)
                     /gene="pth"
                     /locus_tag="ACL8CL_000356"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HGS1329667.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTQVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKKFEKTMQRYNG"
     gene            complement(10402..11517)
                     /gene="ychF"
                     /locus_tag="ACL8CL_000357"
     CDS             complement(10402..11517)
                     /gene="ychF"
                     /locus_tag="ACL8CL_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HGS1329668.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            complement(11601..11795)
                     /locus_tag="ACL8CL_000358"
     CDS             complement(11601..11795)
                     /locus_tag="ACL8CL_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HGS1329669.1"
                     /translation="MYQVGNFVEMKKPHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKINKIID"
     gene            complement(11860..12996)
                     /gene="dnaN"
                     /locus_tag="ACL8CL_000359"
     CDS             complement(11860..12996)
                     /gene="dnaN"
                     /locus_tag="ACL8CL_000359"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HGS1329670.1"
                     /translation="MIHFSINKNLFLQALNTTKRAISSKNAIPILSTVKIDVTNEGIT
                     LIGSNGQISIENFISQKNEDAGLLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQN
                     QIVLTSGKSEITLKGKDSEQYPRIQEISASTPLILETKLLKKIINETAFAASTQESRP
                     ILTGVHFVLSQHKELKTVATDSHRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDD
                     IETVEIFFANNQILFRSENISFYTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSME
                     RARLLSSATQNGTVKLEIKDGVVSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYL
                     IDSLKALNSEKVTISFISAVRPFTLVPADTDEDFMQLITPVRTN"
     gene            complement(13155..14516)
                     /gene="dnaA"
                     /locus_tag="ACL8CL_000360"
     CDS             complement(13155..14516)
                     /gene="dnaA"
                     /locus_tag="ACL8CL_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HGS1329671.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYNYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
     gene            complement(14729..15487)
                     /locus_tag="ACL8CL_000361"
     CDS             complement(14729..15487)
                     /locus_tag="ACL8CL_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HGS1329672.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNHFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIISFSNQEEYSRIINSLK"
     gene            complement(15545..16726)
                     /locus_tag="ACL8CL_000362"
     CDS             complement(15545..16726)
                     /locus_tag="ACL8CL_000362"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HGS1329673.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            16909..17388
                     /gene="rlmH"
                     /locus_tag="ACL8CL_000363"
     CDS             16909..17388
                     /gene="rlmH"
                     /locus_tag="ACL8CL_000363"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HGS1329674.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            17432..17505
                     /locus_tag="ACL8CL_000364"
     tRNA            17432..17505
                     /locus_tag="ACL8CL_000364"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17466..17468,aa:Arg,seq:tct)
     gene            17670..17795
                     /gene="comC"
                     /locus_tag="ACL8CL_000365"
     CDS             17670..17795
                     /gene="comC"
                     /locus_tag="ACL8CL_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HGS1329675.1"
                     /translation="MKNTVKLEQFVALKEKDLQKIKGGEMRLSKFFRDFILQRKK"
     gene            17816..19141
                     /gene="comD"
                     /locus_tag="ACL8CL_000366"
     CDS             17816..19141
                     /gene="comD"
                     /locus_tag="ACL8CL_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system sensor histidine kinase ComD"
                     /protein_id="HGS1329676.1"
                     /translation="MDLFGFGTVIVHFLIISHSYHFICKGQINRKELFVFGAYTLLTE
                     IVFDFPLYILYLDGLGIERFLFPLGLYSYFRWMKQYERDRGLFLSLLLSLLYESTHNF
                     LSVTFSSITGDNFVLQYHFPFFFVVTVLTYFVTLKIIYYFHLELAYFDEDYLYPFLKK
                     VFFALLLLHIVSFVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKFQMEKEIA
                     LKQKKFEQKHLQNYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRIYNEV
                     LVKANHKLRSDKYTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIE
                     LLDLVRIMSVLLNNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFA
                     LGFSTKGRNRGVGLNNVKELLDKYNNIILETEMEGSTFRQIIRFKREFE"
     gene            19138..19890
                     /gene="comE"
                     /locus_tag="ACL8CL_000367"
     CDS             19138..19890
                     /gene="comE"
                     /locus_tag="ACL8CL_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence system response regulator
                     transcription factor ComE"
                     /protein_id="HGS1329677.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNIPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQV
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHTQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
     gene            19942..20013
                     /locus_tag="ACL8CL_000368"
     tRNA            19942..20013
                     /locus_tag="ACL8CL_000368"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19975..19977,aa:Glu,seq:ttc)
     gene            20019..20092
                     /locus_tag="ACL8CL_000369"
     tRNA            20019..20092
                     /locus_tag="ACL8CL_000369"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20053..20055,aa:Asn,seq:gtt)
     gene            complement(20133..20675)
                     /locus_tag="ACL8CL_000370"
     CDS             complement(20133..20675)
                     /locus_tag="ACL8CL_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGS1329678.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            20854..22457
                     /locus_tag="ACL8CL_000371"
                     /pseudo
     CDS             20854..22457
                     /locus_tag="ACL8CL_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip family protein"
     gene            complement(22487..25039)
                     /locus_tag="ACL8CL_000372"
     CDS             complement(22487..25039)
                     /locus_tag="ACL8CL_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HGS1329679.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLAPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYALMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTSLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYIIFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSAYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVNFDY
                     FSPIPYEKTEKIENTNDLISVTSSSNEDAAIQYQIEVPENSQVYLSFINLHFSNDKQK
                     KVDILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTK
                     TFTEAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQ
                     AQTGFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(25101..26723)
                     /locus_tag="ACL8CL_000373"
     CDS             complement(25101..26723)
                     /locus_tag="ACL8CL_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1329680.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
     gene            26929..27954
                     /gene="trpS"
                     /locus_tag="ACL8CL_000374"
     CDS             26929..27954
                     /gene="trpS"
                     /locus_tag="ACL8CL_000374"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HGS1329681.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            28106..29584
                     /gene="guaB"
                     /locus_tag="ACL8CL_000375"
     CDS             28106..29584
                     /gene="guaB"
                     /locus_tag="ACL8CL_000375"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HGS1329682.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(29635..30732)
                     /gene="recF"
                     /locus_tag="ACL8CL_000376"
     CDS             complement(29635..30732)
                     /gene="recF"
                     /locus_tag="ACL8CL_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HGS1329683.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTRTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSVQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNTTDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(30735..31103)
                     /gene="yaaA"
                     /locus_tag="ACL8CL_000377"
     CDS             complement(30735..31103)
                     /gene="yaaA"
                     /locus_tag="ACL8CL_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HGS1329684.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            31262..32512
                     /locus_tag="ACL8CL_000378"
     CDS             31262..32512
                     /locus_tag="ACL8CL_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGS1329685.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPAVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNALFGKSSADFKGWIAKLEQV
                     DKDAICRAANNVKLQAIYFMEGIE"
     gene            32509..33792
                     /locus_tag="ACL8CL_000379"
     CDS             32509..33792
                     /locus_tag="ACL8CL_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HGS1329686.1"
                     /translation="MTKVVFEKKYYPAVKEMIYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSVDTFVTEVDGDVKQYPAGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     RHHILLKLLFAMMFSWTSDRFQKCYESGKIDASLSLEVEVTSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHHLIDDGDIVDFTIFPS"
     gene            33834..34655
                     /gene="rodZ"
                     /locus_tag="ACL8CL_000380"
     CDS             33834..34655
                     /gene="rodZ"
                     /locus_tag="ACL8CL_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HGS1329687.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            34666..35211
                     /gene="pgsA"
                     /locus_tag="ACL8CL_000381"
     CDS             34666..35211
                     /gene="pgsA"
                     /locus_tag="ACL8CL_000381"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HGS1329688.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            35208..36035
                     /locus_tag="ACL8CL_000382"
     CDS             35208..36035
                     /locus_tag="ACL8CL_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HGS1329689.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            36020..36859
                     /locus_tag="ACL8CL_000383"
     CDS             36020..36859
                     /locus_tag="ACL8CL_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HGS1329690.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            36852..37646
                     /locus_tag="ACL8CL_000384"
     CDS             36852..37646
                     /locus_tag="ACL8CL_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS1329691.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            37705..38523
                     /gene="mreC"
                     /locus_tag="ACL8CL_000385"
     CDS             37705..38523
                     /gene="mreC"
                     /locus_tag="ACL8CL_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HGS1329692.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            38523..39017
                     /gene="mreD"
                     /locus_tag="ACL8CL_000386"
     CDS             38523..39017
                     /gene="mreD"
                     /locus_tag="ACL8CL_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HGS1329693.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            39111..40289
                     /gene="pcsB"
                     /locus_tag="ACL8CL_000387"
     CDS             39111..40289
                     /gene="pcsB"
                     /locus_tag="ACL8CL_000387"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan hydrolase PcsB"
                     /protein_id="HGS1329694.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            40513..41292
                     /gene="rpsB"
                     /locus_tag="ACL8CL_000388"
     CDS             40513..41292
                     /gene="rpsB"
                     /locus_tag="ACL8CL_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HGS1329695.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            41371..42411
                     /gene="tsf"
                     /locus_tag="ACL8CL_000389"
     CDS             41371..42411
                     /gene="tsf"
                     /locus_tag="ACL8CL_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HGS1329696.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            42657..42980
                     /gene="tnpB"
                     /locus_tag="ACL8CL_000390"
     CDS             42657..42980
                     /gene="tnpB"
                     /locus_tag="ACL8CL_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS1329697.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(43121..43408)
                     /locus_tag="ACL8CL_000391"
                     /pseudo
     CDS             complement(43121..43408)
                     /locus_tag="ACL8CL_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            43390..43919
                     /locus_tag="ACL8CL_000392"
                     /pseudo
     CDS             43390..43919
                     /locus_tag="ACL8CL_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_050084071.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(43994..44914)
                     /gene="cysK"
                     /locus_tag="ACL8CL_000393"
     CDS             complement(43994..44914)
                     /gene="cysK"
                     /locus_tag="ACL8CL_000393"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HGS1329698.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(45163..45798)
                     /locus_tag="ACL8CL_000394"
     CDS             complement(45163..45798)
                     /locus_tag="ACL8CL_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HGS1329699.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHGLMEL
                     DTNFTDQVDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            45854..47152
                     /locus_tag="ACL8CL_000395"
     CDS             45854..47152
                     /locus_tag="ACL8CL_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1329700.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     STILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQVVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            47149..47811
                     /locus_tag="ACL8CL_000396"
     CDS             47149..47811
                     /locus_tag="ACL8CL_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HGS1329701.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQFWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            47891..48439
                     /gene="raiA"
                     /locus_tag="ACL8CL_000397"
     CDS             47891..48439
                     /gene="raiA"
                     /locus_tag="ACL8CL_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HGS1329702.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            48575..50548
                     /locus_tag="ACL8CL_000398"
     CDS             48575..50548
                     /locus_tag="ACL8CL_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HGS1329703.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDETSESDISQINSFVANFISEFSEKHMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGRKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            50545..50997
                     /gene="rplI"
                     /locus_tag="ACL8CL_000399"
     CDS             50545..50997
                     /gene="rplI"
                     /locus_tag="ACL8CL_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HGS1329704.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            51041..52393
                     /gene="dnaB"
                     /locus_tag="ACL8CL_000400"
     CDS             51041..52393
                     /gene="dnaB"
                     /locus_tag="ACL8CL_000400"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HGS1329705.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            52395..52661
                     /locus_tag="ACL8CL_000401"
     CDS             52395..52661
                     /locus_tag="ACL8CL_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HGS1329706.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGDVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            52750..54096
                     /gene="cbpD"
                     /locus_tag="ACL8CL_000402"
     CDS             52750..54096
                     /gene="cbpD"
                     /locus_tag="ACL8CL_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HGS1329707.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRI
                     IKANTMTGFIHFKDLDGGSVGNSQSSTSTGETHYFKTKSAIKTEPLVSATVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSATVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENGSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      54179..54281
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            54352..54642
                     /locus_tag="ACL8CL_000403"
     CDS             54352..54642
                     /locus_tag="ACL8CL_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HGS1329708.1"
                     /translation="MKASIALQVLPLVQGIDRIAVIDQVIAYLQTQEVPMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYTETTH"
     gene            54605..55363
                     /locus_tag="ACL8CL_000404"
     CDS             54605..55363
                     /locus_tag="ACL8CL_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1329709.1"
                     /translation="MRNLRSILRRHISLLGFLGVLSIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            55402..56409
                     /locus_tag="ACL8CL_000405"
     CDS             55402..56409
                     /locus_tag="ACL8CL_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1329710.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEASKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            56409..57137
                     /locus_tag="ACL8CL_000406"
     CDS             56409..57137
                     /locus_tag="ACL8CL_000406"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329711.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            57256..57714
                     /locus_tag="ACL8CL_000407"
     CDS             57256..57714
                     /locus_tag="ACL8CL_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HGS1329712.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRELLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQIIQEVDRKGK"
     gene            57716..60148
                     /locus_tag="ACL8CL_000408"
     CDS             57716..60148
                     /locus_tag="ACL8CL_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HGS1329713.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVYAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSGKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
BASE COUNT        18681 a        10385 c        11854 g        19293 t
ORIGIN      
        1 tcttcatagg gttctttatc gtatttacgc ttctgacttt cctgcttacg gatatagtct
       61 aaaattcgat ttcgaaactt ggttttgaaa tatttcctta aacgtggaat atcgtctacc
      121 agtccttctt ctctactaat caattcatgt aagcagagca tgccttcttg gtcccaatcc
      181 gacaattccc ataaatgaag gtaatattca tttctacact tatacacagt cccttggact
      241 tcttcataca attctttaat cataattttc ccctttctag atacagtcta acagattaga
      301 aaacacattt gttccatttt ctccattctg ttccctttag ctcctaaact gcacaaaaaa
      361 gagaggctcc agcctctctc agggttattt ttcgtcattc atttttgact ttacttcatc
      421 ataggatagt gcatgagatt cctcttctac tgcttcaggc atctttcctg tttcgtaaag
      481 agctttaatt tgtgtactat ccaatgtttc gtatttcaat aatgcttctg caattaactt
      541 gtgagtttca cgatttgact gaataatttc agcagcttta tttcgtgcct catttaataa
      601 tgaacgaacc tcttcatcaa tttcataagc tgtttgttct gaaattgatt tttgaggact
      661 ctgtgcacca agcatagcat ggtttccttc atattgtact gggccaagtt tttcactcat
      721 accgtactct gtaaccattg cacgtgccat ttgtgtcgct tgttcaaagt cgtttgaagc
      781 tcctgtggtt tggacattaa agataatttc ttcagctaca cgtccaccca ttaagccagc
      841 caattgctct ttcatatctt ctttagatag aagcatttga tcctctttag gaagtgcaat
      901 catgtatccg cctgcacggc cgcgtggtac aattgtaacc ttatggacaa cgcgagcatt
      961 cgacaagact agaccaacaa tggtatgtcc tgcctcatgg taagcaacca attctcgttc
     1021 tttttgtgaa actgtcttat ctttcttaga aggtccagca ataactctat cttctgcttc
     1081 atcaatatct gaagcatcaa ttatcgattt attgcgacga gcagcaacta aagctgcttc
     1141 attcaagaca ttctctaaat cagcaccaac aaagcctgga gtttgttgag ccactaattt
     1201 caaatcaaca tcttctgcta aaggcttatt cttagcgtga actttcaaga ttgcttcacg
     1261 acctttaaca tcaggacgac caaccaatac ttttctatca aaacgtcctg gacgcaaaag
     1321 ggcagggtca agtacatctg aacggtttgt cgcagcgatg acgataatcc cttcatttcc
     1381 ctcaaaacca tccatctcaa tcaaaagttg gttcaaggtt tgttcacgtt cgtcattacc
     1441 tccgccgaga ccgactccac gttgacgtcc aacagcatca atttcatcga taaagatgat
     1501 agctggtgct gcttttttgg catcctcaaa aagagagcga acacgactag ctccaactcc
     1561 gacaaacatt tctacaaagt cagaacctga gatactaaag aatggaacac ctgcttctcc
     1621 agcgactgcc ttagcaagca aagttttacc tgtccccgga ggtccctcca aaagaacacc
     1681 tgctggaata cgggctccaa gttttgtgaa tcgttttgga tcttttaaga actcaacaac
     1741 ttcaactagt tcttgttttt cttcttcagc tccagcaaca tctgaaaatc ttactttaat
     1801 atcttcttta tttgctgctt tagccttact acgtccaaaa ctcattggat tacggccatt
     1861 gcctcctccc atatttccca tcatagagaa taggaagaag aatagaattc caaatggcac
     1921 aatggatacg agtagattaa tccatatacc acttgaactt tcatgcttaa cagttacttc
     1981 tgctttatgg tcagtagcaa gtttttgcaa ttctgatacg gtagtatctg caggaagaat
     2041 agtgctggta aatttctcta ccttagtaac agatggcgtg aaaaactgaa tacctgttcc
     2101 ttctttactt gttttaggat ttttatagac accagaaact tcgataacac taccatttgg
     2161 ttggtaagtt aattctttta cattaccatc ggtaatttct tgtaccaact cagtatagtt
     2221 gatttgctga cttcctcctg agttattccc agaatagaaa tactggaatc ctgtcacaag
     2281 gaaaaagata aataataacc atagaaaagg atttttaatt aaaccattat tttgtttttt
     2341 cattaaagat tattctttct aatttgaata cacttcctct ttcaatactc caatataagg
     2401 aagattacga taattttctt tgtagtctaa accataacct actacaaact catttgggat
     2461 agtaaagcag gtatagtctg cctcaatttc tacaacacgt ccttctggtt tatccaacaa
     2521 ggttgcaatt ttaacagaag ctgcttctct ttctttaaac atatctcgca aattcttcaa
     2581 agtttgacct gtatcaatga tatcttctac aaatagaaca tgtcttcctt tgatatcttg
     2641 agtcacatct tgtttaatat tgataacacc actacttgct gttccaccat ggtagctaga
     2701 aaccatcatg aagtccattt caatatgtgt atcaatatgt ttgaccaatt cagccataaa
     2761 aggaatagat ccttttaaaa tcccaactaa gattggattt tttcctgcat agtctttagt
     2821 taattgagca cctagttttt tagctgcttc tgtaatttca tcgtgtgaaa cgaggacttt
     2881 tttaatatcg ttttctaaca ttttttacct atctattttt tctatataaa gtacagtgtt
     2941 cattatatca tttttcgttt ttttactcaa attattggtc gcaattccca aaattgagac
     3001 aatttcacca aattgctcaa taataagagc agagtttctc ttttccatag ggattttcaa
     3061 atcaataaat aaacgtctga gtttttttct atgcccattt tgaatcaaaa catctcctgt
     3121 ttttcgatga cgaatgtgta tggatgtttc acgtgaaaca ggtatttgtt gaattaattc
     3181 accttctaat ggaagtccaa aggaaaataa atatccttga taagctacct gattttgata
     3241 gtgtaacaca agttcatctt ccttttcatc agcctgcgga ctgattttac aaatctgaaa
     3301 ctgttggtac tcttttatca attcatagcc atttttaatc ggatgacgat actggctttt
     3361 agattttaaa atctgctgaa cttcagcaaa ctgagctttt gtaagattca aatctggaaa
     3421 acgattcaga taagtttgaa gtaaaactct ttgtgtagac tcagagtaag aaaataactg
     3481 ctgtaaatct tccacattaa tattgttaga taattcagct attgccaaat cataatctaa
     3541 aatttcattg ccaatgccta agattgcatc cctaaatcga ggattttctt tttccaattc
     3601 tggtaagtaa gagtttcgaa tacgatttcg aaaataatga ttctcctgat ttgatgtatc
     3661 ttcaaagtga aaaattgatg gaaagtcttt tttctgaaaa tgcaagaagg gacgaatgat
     3721 ttctatctct ccgactactt gcttctcctt aattcctgat agatagcgca agcgagttcc
     3781 tcgaatcaag cgcatcaaaa tcgtttccac ctgatcatca gcatggtggg cagtgactaa
     3841 agctgtcgca cctgtctttt tcataacctc ttgaaaaaaa tcataacgaa aatttcgtgc
     3901 acgcgcttct gaaaattctc ctgaaaaatt gctgatataa ataggaagct ctgcttcagc
     3961 agccaacttc cttaattcct tttcttccca atctgattca attctctgct tatgattcac
     4021 atgagccaga atcaattcaa tctctaactc tttttgataa gtagacaata ccttaaatag
     4081 aaacatagaa tctaatccac cagaaagagc tagaaccgcc ttagcatgct ttttgaaata
     4141 tcccttcttg agaaaatgat ttaaaaaatc tggttccctc attttagaac ctcataaaca
     4201 tccttggcta tcttagaaat cgtatcataa tcagaattct tagtgaaaat agaaagaata
     4261 aatggagaat ctgcatagac aacacccgta tcatgcttaa attcatccgc atctccaatt
     4321 ttatgagcta ctttaacaga aacacctttg gcaattcgct gactatcaaa atctgtttta
     4381 gtcaaagact ctagcacaaa tccattttga ttataaatag cttccataaa cttcccggcc
     4441 atcttagaag aaatcaattt ttcttttgga tcccaatcat ctcccataat ggcagacatc
     4501 ttggatttga atgtggcatc agattggttt gaaatgtaat atcccaatag attatgagct
     4561 acattatcag attcttttga tactttcgta attaaatcct ttaaagaata ttctttatta
     4621 tcttcttttt taggaagact accacttccc tctggtttat aagaacctgg aaaatcattg
     4681 actgcagata cgtattttac agtcgtatct aactgataaa gaccctcatt tattttttct
     4741 tgcgtataat agagatagga gagtttcaaa acgctggctg catacatctt ttcatattga
     4801 ttgataccag cttcttttcc agtagtcagt tgcttaacat aaatagagaa agaatctttc
     4861 tgatattttt cagataacat ttcttgaact ttactcatcc gattatcttc ttcagaagtt
     4921 gattctttag ctacccatcc agcctgatca atatgtagaa attctcttcc ttctacaaac
     4981 atggtcttgt cgattgatac ttgcgaataa gctgataagg atgatttcac ttcttttaaa
     5041 tcataaggac tattgtacag tttaaagtca gattctaacc atactttttt tattgttgga
     5101 gttacctctg attgatcata taaaaatcgt ttgtccgcag ctataaattg atgatttgat
     5161 agcttaaata ctggaattcc ttgtttattt aagcgccact cggttatttg aaaacttgtt
     5221 ttgggagtca atttcccaga ttccactact aaatcttcat tcgcataaac aggaacctct
     5281 ccataaacca tgggagaact taatttttct ctaaaataaa taccaaagtc agattgtgaa
     5341 aggtaataaa tttctttcga agtatagacg acttcttttt ctgtgctaac gacttttgaa
     5401 atggtcaaaa aacttggtag caacaaaata attaagaatt tacgcattct tcctttcctt
     5461 ttcttcttgt aaacgcaata attgattttt atcagccaat tcttccagct tttcatctga
     5521 caaactcaaa aattgctcta ttacgtctaa taaattttcc attttatcac ctttgaagca
     5581 agtcaggaat cgtataaact ttttccctcg acttagaata atagtacttc gctcgtgtat
     5641 atttagcagc ataatcttca tctttcaact tggtagcaaa tgctgtctcc ttatcctttt
     5701 catcactcaa agtttgatac tgagtttgca agtctgctaa ttgctgacgt ctttggagta
     5761 attgctgata actctgcgct aaattaaaag ttggcaagat aaataatagc ataatcaaaa
     5821 tcaatacccc tcccataaaa cgattccgtt tttgtcgttc tttcatcagg tagcgacgac
     5881 gttggtattc attttgaata aaagaattat tcaattgtac aatattttta gacattttct
     5941 tctacccgtg tttcactgat aatttcatac attcctgctg catcttcttt ttttgtacta
     6001 tctttcatct ctagtacttt tacaagcagc aacttattgc caaagcgaat ttcaacttgg
     6061 tcattaactt tcaagtccgt tgaacttttg gccaagattc cattaacctt gattctacct
     6121 ttatctgcta cttcctttgc gactgtacga cgcttgataa ttcgcgatac ttttaaatat
     6181 ttatctaatc tcatccttat tacctcaatt tattattgta ccatttttat ctattttata
     6241 gaagaaaaat atcaaatgga attttcttcc ttagactctt ttatctctaa taaactttct
     6301 ccaaaaatca gcaaaccttc taaaatttca taatctttct tattttggac atcaaataca
     6361 agctccatta atcccttatt ctcagcgatg cctgctttta agttcgttac ggataaagct
     6421 ttaaaataat cttgagctaa aaacagtcgt tgagtgactt tttcaaattg aattgtaatt
     6481 ttattatctt ttctttccac acgttgaaca aagaccttgt ccaagtatga tttgaccaaa
     6541 ccaatctcta acagataggc tactacatct gggtattctc caaaacggtc tatcaactcc
     6601 tcttgtaact cttcataatt gacacggttg tcaatttgac gaattttctt gtaaatttca
     6661 atcttatgtc gttgatcaga aatataagta tcaggaagat aggcatcaat ttgcaaaatc
     6721 aactcagcat tccctttgat tcttgtgtta gcgttagcat taccgtttcg tttagcaata
     6781 gcttcctcta ataactgcga atacaattca aaaccaacag aatcaatgaa accagactgg
     6841 gattttccta aaagatttcc tgctccacga atcgaaagat ctcgcattgc aatcttaaag
     6901 ccagagccca attctgtaaa tcctttaatc gcttctaatc tcttttcaga gacttcactg
     6961 attgattttt ctggacgata catgagataa gcataagcaa tacgattact acgaccgact
     7021 cttcctctta actgatataa ggttgacaag cccatatggt ccgcattttc aataaataaa
     7081 gtattagcat ttggaatgtc cacccctgtc tcaataatag tagtcgtcac caagatatcg
     7141 tattgtccct caataaagtc taatagagta ttttccaact ggacttcact cattcgacca
     7201 tgaacatatc caatcgaagc ctctggaatc aactcctgta attctgaaac cttctgaaca
     7261 attgtgtcaa ctttgttgta aagatagtaa ccttgacctc cacgctccat ttcacgcaag
     7321 acagcatcac gaatgacact atcattcttt tccaaaacat aggtctgaac agggtagcga
     7381 ttagtcggcg gagtttcaat aacagacaaa tctctgattc ccagcataga catatggagg
     7441 gtacgaggga ttggcgtagc ggtcaaggtt aggacatcca cttgtttctt cagttctttc
     7501 aaagtttcct tatgcttgac accaaatcgc tgttcctcat caataatcat caagcccaaa
     7561 tcagcaaaca caacatcttt tgacaaaaca cgatgtgttc caatcaaaat atcgacttga
     7621 ccgtttttca atttttcaag tgttgcagtc tgctcttttt tacttctaaa gcgactcaac
     7681 acatcaacat taactgcaaa attttggaat cgttccttaa aattcgtata gtgctgttgc
     7741 gctaaaaccg tcgtcggaac tagaatgaca acctgtttgt gatcattgac tgctttaaag
     7801 gctgcacgca tagcaacttc agtctttcca aaaccaacat ccccaactaa aagtcgatcc
     7861 attggctgag aagcctgcat atccctcttg atttcctcaa tactacgaag ttgatcatcc
     7921 gtttcaacat aagggaaagc atcatcaaag gcatcttgat catcatcatc agctgagaaa
     7981 gcaaaaccct tcaactgact acgttcagag tagagtttga ttaaatcatc agctatatcc
     8041 tctacctggt tcttaacctt ttgcttggcc tttttaaaat gaccgtcatt taatttattg
     8101 agttttggag ctttaccatc acttgaaata tatttggaca gtagatgaat ctgttccacg
     8161 ggaatagaaa tttgatcacc attttggtac tggacactga cataatcacg atgaatttcc
     8221 ttgatttcaa tggtttcaat tcctagatat tgaccaatcc catggatatg atggacaaca
     8281 tagtcccctt tttcaagttc attgtaatct tttaatctct ctgcatttga aacatgttgt
     8341 cttcgaaaac gacgctttaa tttcttttga aaaatctcat gttcagttat caataaaatc
     8401 ttttcatcta caaaatgaaa accatgtctg agattaccct ctattaaatt tacagattct
     8461 ttacagatac ttgtcttatc tctagaatcc aatttaatct gatattcctc taacatatcc
     8521 tccaatgttt tacttcccat tgaattgcta gactgcagaa taatggtgta atccattttt
     8581 ttatatcgtt caatttcttc ttttagaaaa gaaaactgat tgaaaaattc ctgcatagga
     8641 tattgattga attgataaat tttgtcaaat ttgagatttc ctaaaccctt ttgaagatta
     8701 gagaaaaagg tcactggact ttgtttttta tagatttgtt caatatcaga aaaatactgc
     8761 atatcagaaa atgctttact attctgtaat tcttctgtaa agtactgcgc taagtctctt
     8821 tcaaagactt catactgatt catcaatttt tgataatcat caaagaatat tggagtatct
     8881 ttttcaatat aatcaaagac agtccatgtc ttatcatagc acaaagataa aaacttccga
     8941 gagtctgcat gactttgttt ttggtgaaaa cttgaaagaa tttcttctag gtatgatttc
     9001 aaaataggtg ataaagtttt tgaaatttgt ttttctaaag ctgactgtcc tcgttgataa
     9061 tccttttctc tcaaaagcat atcactagct ggaaagatag tgagttctgt cttattttct
     9121 ttcgataatt gtgtttctac ttcaaatgac ctgataccat caatttcatc accaaaaaac
     9181 tcaattcgac aaggttctaa ctgggatatt tcaaaaatat ctaaaatatc tcctcgaaga
     9241 ctaaattcgc cctgagtttg tacttgagta acttttcgat agccattttc ctttaactga
     9301 tggataaacg cgtgttgatc atattcttca ccaactgaga tttttacaat actatctttg
     9361 aatgcattgg gagacggtaa aatcaatcga cttgctgcga tattacaaac taaaatccct
     9421 ttcttagatg aatcagtcaa aaaacgcaag gcttcaaccc gtgaaataat tttttcctgt
     9481 gaagacatca aaaactccac cataggagca tcatctacca aaaatggata gacgagttcc
     9541 tcacccaaga tagaaataag atcactaaca agtccttctg cttctccata agttgacgtc
     9601 aataacacaa tcctatcttc tttttctaaa ctgcttgcaa ttgcaagagc cttagtagat
     9661 gttgataaac caagtattag ttgtcttttc ttatctgtta aattttgatg ccatttttta
     9721 atctgatcat tttctgagaa taaatctaat aaggtcacca tttatccgtt atacctctgc
     9781 attgttttct caaatttttt ctcttgtaaa tagtagttta cagaatcgtc aactttgtca
     9841 acagactgta aaataccgat ataatcatcc ctgtcaaact tactcaaaac atgatgaaca
     9901 actgacatac catttttagg tcttccaatt ccaatcttaa cacggttaaa gacctgagtt
     9961 cctatatgtt gaataataga cttgatacca ttatgaccac ctgctgagcc ttttgctctt
    10021 aaacgaattt tcccaacttc catgtcaaga tcatcgtaaa tgataagtaa atcgtcaata
    10081 tccaaaccat agtaagttaa taaagcatga actgcttttc cactttcatt cataaaggtc
    10141 gttggtttaa ccagataaat tttttctcca tttaggaaaa aggatgctag gtcagcttga
    10201 aatatcttat cgtgtgtaaa agtgacattc tgtttcttcg ctagttgatc aatcaacata
    10261 aaaccaacat tgtgttttgt ttcaaaatat ttatcccctg gatttcccaa gcctacaagt
    10321 aatttggtca tttatttttc ctttcaaaag ccaaaagggt tggaattttt ttccaaccta
    10381 attgacacca tttattaatt tttagacatt aaagcggaat tccatgatgt cgccatcttg
    10441 aacgatatat tcttttcctt cttcacgcaa gcgtccagct tcttttacgg ccttttcaga
    10501 tccgtatttc actagatctt catatgacat ggttactgca cgaataaagc ctttttcaaa
    10561 gtctgagtgg ataataccag ctgcttgagg agccttcata ccacgtttga aagtccaagc
    10621 gcgaacttct ttttcaccag ctgtgaagta agttcccaat ccaagcaagt ggtaagctgc
    10681 acgcgtcaac ttatctacac ctgattctgt caaaccaatt gcttcaagaa actctttttt
    10741 atcttcatca tccaattcag aaatttcttc ctcagcacgc gcagaaataa cgactacttc
    10801 agcattttct gtcgctgcaa attcacgaat ttgtttgaca tagtcgatag agtcaggttc
    10861 tgaaaccaca tcctcgtcca cattagctac ataaagaact ggtttagtcg tcaaaaggaa
    10921 aagacctttg acaacctttt gttcctcatc tgtaaattca atggtacgag ctgatttccc
    10981 gtcttctagg actggtttaa tcttttgaag aacattgaat tctgctactg attctttatc
    11041 tttttgcgta cgtgccatct tttctacacg cgcatatcgt ttgttcactg attctaagtc
    11101 agcaagaatt aattccagat taattgtatc aatatctgca agtggatcta caaaggcgtc
    11161 ttcacgtcct tgctcgcgca tcacattttc atcatcaaaa gcacgaacta cgtgaacaat
    11221 cgcatctact tcacgaatat tggccaagaa tttattccct agcccttctc cttttgaagc
    11281 tccttttaca atccctgcaa tatccgtaaa ttcaaatgtt gtgggaactg tctttttagg
    11341 agttatcatt tcagttagtt tttgtaggcg ttcatctgga acttccacca ttccaacatt
    11401 tggatcaata gtcgcaaatg ggtagtttgc tgcctctgct cctgcttttg taattgcatt
    11461 aaatagtgtt gatttaccaa cgtttggcaa accaacgata cctgctgtca aagccatatt
    11521 tctcattctc cgttttcatt tcaatcccta atattataac acaaaaagag ataaagtgct
    11581 aaccctctaa ctaagggttc tcagtcaata attttattta tttttcgctc aaaatcatat
    11641 cgccccatca tgacaacatg ctcacaatta ctgcatttta ttttgatatc tgctcctaca
    11701 cgtgtaattt cccaacgatt agccttttta ccagtcgact tgattgtaca agcgtgtggt
    11761 tttttcatct caacaaaatt tccaacttga tacatactac tctccttttc tttttcgatt
    11821 atatcataaa agaggcgagc caggctcaac ctctttcact taatttgtac gaactggtgt
    11881 aatgagctgc atgaagtctt cgtcagtatc tgctggcaca agagtaaatg gacgaacagc
    11941 tgagataaag ctaatagtca ccttttcgct atttaaagct ttaagagaat caatcaagta
    12001 agttgggttg aaactaatgg tcaaatcttc accagtaacc tgatcagtat cgatttcttc
    12061 gtttacttta ccaacttctg gagagtgaac atgggcgcta acaaccccat ccttaatttc
    12121 aagtttcaca gtaccatttt gagtcgcact tgataaaaga cgggcacgct ccattgactg
    12181 gcgtaagttt accacattaa aagtaatagt agtgttaaag tctgttggaa tcaagcgatc
    12241 tgtatcagga tagtttcctt ctaggagacg agtatagaag ctaatatttt cgcttctaaa
    12301 gaggatttgg ttattggcaa agaaaatctc tacagtttcg atatcatctg taaataccgc
    12361 tgaaaattcg cgtagagaac ggctaggaat tacgacatca aaatcatcac tatttttttc
    12421 aagagtcaat tttttctggc ttaggcgatg agagtctgtt gcaactgttt ttaactcttt
    12481 gtgttgactc aatacgaagt ggacacctgt taaaatcgga cgactctctt gtgtacttgc
    12541 agcaaaggct gtttcattaa taattttctt gagtaatttt gtttcaagta ttaaaggagt
    12601 gcttgctgaa atttcttgga ttcgtggata ttgttcgcta tcttttcctt ttagggtaat
    12661 ttctgatttg ccactggtta aaacaatttg attttgttca atttctttaa aatcaagagt
    12721 tacatcaggt aaactagata ctacattgat aaagaaagaa gcttcaagaa ggatcgaacc
    12781 taaagaagta attaacaaac cagcatcttc atttttttga gaaataaaat tttcaattga
    12841 aatttgacca tttgaaccaa ttaaagtaat accttcattg gtcacgtcaa tttttactgt
    12901 tgataaaata ggaatggcat ttttagaact aatagctctc ttagtagtat ttaatgcttg
    12961 tagaaataaa tttttattaa ttgaaaaatg aatcatggat tctcctttat ttatttttag
    13021 tattagaaag ataatagtaa taatagagtc tgtgaaatct gtggaaaaca gcctttaaac
    13081 aagtcatatc aagtttttta gcttgtttaa aaaatgtgga taaaaaagat aaaaaactaa
    13141 aagttatcca caaattattt gattttcttt ttgattgatt caatttctaa acgtaaatta
    13201 tcgtcttgat caatcaaaga ttttattttg gcatgggcat gaatgactgt ggtatgatct
    13261 tttcccccaa attccttccc aatttttgga agactattat ctgttagttc tctagataaa
    13321 tacatggcta cttgacgggc caaaacaata ttttgaaggc gtctacttcc cttcatttct
    13381 ttgatactaa caccataaaa gttaccaact tcagtttgga ttttatcaat tgggatgacg
    13441 agcatttggc taacatcttg tttgcgggct ctaatggctt ctgcagcaat atcaatagtg
    13501 atatccttga ttttttttac tctggcaatt aaagtgatgt cgttgatggc tccctcaaga
    13561 tctcgaacat ttgaatcaaa ttgcccagct aggtattcta gagtatcact ttggaaattg
    13621 tagcctaaat gttccgtttt actttgtaaa atggcaatac gtgtttcaaa gtcagggggt
    13681 gtgatagttt gtgtcaatcc ccaactaaaa cgcgtgacaa gcctctcctc gagcccttct
    13741 agatgttttg gactacgatc actcgttagg acaatctgtt tttgcttgtc atgaagggcg
    13801 ttaaaggtat tgaaaaattc ttcctgagtt gcgacttttt ttccgctgag tgactggata
    13861 tcatcgatta acaaaagatc aagactacga taggtctttt taaacttttc catttcccca
    13921 agtcttaggt gatcaagaaa gtcattaata aagctttcgg cagggatata tttaacacgc
    13981 gcattaggaa tattttttag aatttcattt ccaatagcgt ttaataagtg agtcttacca
    14041 aggcctggtc ctccatagat aaaaagaggg ttataggtca gagccaaatc ttcagagaca
    14101 gctaaagcgg ctgacacagc ccaaacattt ccatcccctt gaataaagtt atcaaaggta
    14161 tacttttctt ttaatcccgt atctgaataa ggaatagata ctaactttgg actatagtta
    14221 taaagagtta aatttgtagc ttcttcaact tgtgagctag tcgtatcttg aggtttggtg
    14281 aaaatatagt ggggagttat ttcagcgtca taaatttcaa aaccagctac tacaataata
    14341 tcttttagtt gtttttccca gaccatttcc atttcagagc gaggtagaaa tatagtggca
    14401 acattttcct ctaccttgat gagttcagct tgaatagcat agaaatcata catggatcga
    14461 gtcagtcttt cttgtgcaaa ttctaatata cgattccaaa attgtttttc tttcaatcct
    14521 ttctcctcct ttactattta aaagtttaat actagagata ttttaccata gatagcaaga
    14581 attttccaca agctgtgaaa aataatccac aatgttacca aactttatcc acaggttggg
    14641 gataaaagaa gaaattattg atttattaag ctttttattg aaaaacatag tggataacct
    14701 tgtgagataa aaaaataaaa gaacagcctt atttcaggct gttgataatt ctactatatt
    14761 cttcttgatt tgaaaaagaa ataatgattt ttccactgtc ttttttagat agtttaattt
    14821 ctacatctaa tccgagtagt tttcttaact gtttttcttc attttgtatg aaatgattag
    14881 ttttttgctg tttcttttgt tttttctctg tcagaagagc ttctaatttc cttacagaaa
    14941 tatcttcttc tataatccgt tgaaagaaat agtcttgttg ttccttattt aacccaacta
    15001 gggaacgcgc atgggcttgt gatagtttgc cattttctac ttctgaaaga atctgttctg
    15061 gcaaggaaag taaacgaatg gagttgctga tatatggacg agacttgccc atcttatctg
    15121 caatttcagc atgggtgaat cctttctcta cgagagattc ataggcgcgt gcttcttcta
    15181 ttgggtttaa attttctctc tgtaaatttt caataatgga ctggaccatc atctcttggt
    15241 ctgaaatctg tttaacaaca gctgggatag accgtagacc agctaaaagt gaagcccgat
    15301 agcgtctctc tcctgcaagg atttcataac caataacagg agattgacga acaataatcg
    15361 gttgaatgac cccattttct ttgatagact gtgctagttc atctagtttt tctctatcaa
    15421 attcttttcg gggttgatag ggattttttt gtatatctgt gatagaaatc atttcaaatt
    15481 tttccatgat tctacactaa cacatctttt ctcttatgta aagctttctt tacatagatg
    15541 tcaattaaga ttctaaatca ccagaactct tgttaagttt gatagaggta gtttcttctt
    15601 tcccgttacg atagtaggtt atcttaatgg tgtctccgat agaatggttg taaagagcac
    15661 tttgtaagtc tgttgatgaa gcaatctctt tgtcatctac ttttgtaatt acatcgtatt
    15721 tttcaaggtg accattggca ggcatattac tttgtaccga acgaacaact acaccagatg
    15781 taacattact tggaatattg agtcttctga tgtcgcttgt actcacatta gataaattaa
    15841 ccatctggat tcccaaagct ggacgcgtca cttttccgtt tttttctaac tgttcaataa
    15901 tattgatagc atcatttgca ggaattgcga aaccaagacc ttctacagat gttcctccat
    15961 ttgtagcaat tttacttgag gtaattccga taacctgccc ttgaatattg atcagtgggc
    16021 cgccagagtt acctgggtta atagcagtat cagtttggat ggcttttgta gaaatagctt
    16081 gtccatcttc cgattttaag gatacatttc tattgagact ggatacgata ccttgagtga
    16141 ctgtatttgc atattcagaa cctaacgggc taccgatggc aatagcagtt tctcctacag
    16201 ttaacttact agaatcacca aactcagcta ctgttgtcac tttttctgaa gagattttta
    16261 cgacagcaat atcagagaaa gtgtcagctc cgacaatttc tccaggtact ttagtcccat
    16321 ctgacaatcg aatatctact ttgctggcgc catttataac gtgattgttg gtgacgatgt
    16381 aagcttcttt atcattcttt ttataaataa ctccagatcc ttcactagag attcgctgag
    16441 aatctgtgtc agtatcatca ttgccaaata cgctattttg tctgtttgcc gaataagtaa
    16501 taacagaaac aacagcatct tttactttgt taacagcctg tgttgttgaa ttttcgttct
    16561 tataggcagt ttgtgtaata gtactattgt tgttagagtt gtttacacta cttttttgag
    16621 ttagttgagt tattgaaaaa ctacccaagg ctccactaaa aaagctaatg acgataacga
    16681 ctaataattg aaaccatttt ttgtaaaatg tttttagatg tttcatattt gcctccatat
    16741 gtttgaatta ctgaaagtat aaactgacta gcttaattat aacttaaaca caaaagtttt
    16801 acacaaactg tggataactc ttttgaaact gtgattttct taattgaaat ctatttttta
    16861 ttttgtgaat aagatgtgaa aaaatagaga atatgttaga atagagtcat gaaaattaaa
    16921 gttgtaacag ttgggaaact gaaagaaaag tatttaaaag atggtatcgc agagtattca
    16981 aaacgaattt ctagatttgc taagtttgaa atgattgagt tatcagatga aaaaacacca
    17041 gataaggcca gtgaatcaga aaatcaaaag attttagaaa tagaaggtca gagaatttta
    17101 tcaaaaattg ctgaccgtga tttcgttatt gtgttagcca ttgaagggaa aactttcttc
    17161 tcagaagaat ttagtaagca gttagaagaa acttctataa aaggattttc tactcttact
    17221 tttattattg ggggaagttt aggattgtca tcatctgtaa aaaatagagc caatctttct
    17281 gtcagttttg gtcgcctaac cttaccccat cagttaatga gactagttct tgttgaacaa
    17341 atctatcgcg cttttacgat tcagcaggga ttcccctacc ataaatagaa aattgacttt
    17401 taattgaatt tttggtagaa taattgtatt aggtctcata gctcagctgg atagagcatt
    17461 cgccttctaa gcgaacggtc gcaggttcga atcctgctgg gatcaatata atagcaaagc
    17521 tgggaatttt cccggctttt ttcttaaaaa agtacacttt gggagaaaaa aatgacagtt
    17581 gagagaattt tatctaaaac gaaattccat tttgtataat ggtttttgta agttagctta
    17641 caagaaaaaa cattttagga gattttatta tgaaaaacac agttaaattg gaacagtttg
    17701 tagctttgaa ggaaaaagac ttacaaaaga ttaaaggtgg ggagatgagg ttgtcaaaat
    17761 tcttccgtga ttttatttta caaagaaaaa agtaatgaaa taaggggaaa gagtaatgga
    17821 tttatttgga tttgggacgg ttattgttca ttttttaatt attagtcaca gttaccattt
    17881 tatttgtaaa ggtcaaataa atagaaaaga attattcgtt tttggtgctt atacattact
    17941 aacagaaata gtatttgatt ttcccttata tattctatat ttagatgggt tagggattga
    18001 aagattttta tttcctttgg gattatattc ctattttcga tggatgaaac agtatgagag
    18061 ggatagagga ctattcctaa gtttactact atctctttta tatgagagca ctcataactt
    18121 tctgtccgta actttctcct ctataacagg agataatttt gttttacaat atcatttccc
    18181 attctttttc gttgtgacgg tgttaaccta ttttgttaca ttaaaaatca tttactattt
    18241 ccatttggaa ctagcctatt ttgacgagga ctacctttat cctttcttga aaaaagtatt
    18301 ttttgcttta ctattgctac atattgtatc tttcgtttca gatatggtaa gtacgattaa
    18361 acatttgaat agttttggaa gtattttgtc atctattgtc tttatctctc tccttttgac
    18421 cttctttgca atgaattctc ataaatttca aatggagaaa gagattgctt tgaagcagaa
    18481 gaaatttgaa cagaaacatt tacagaatta cacagatgaa attgttggtc tgtataatga
    18541 aatccgtggt tttcgacatg attatgctgg aatgcttgtc agcatgcaga tggcaattga
    18601 cagtggtaat ttacaggaaa ttgacagaat ttacaatgaa gttttagtca aagcaaatca
    18661 taaattgcgt tcagataagt acacttactt tgatttgaac aacatagaag actcagcttt
    18721 acgaagtttg gttgctcagt caattgtcta tgctcgaaat aatggtgtag agtttacact
    18781 ggaagtaaaa gatacgatta ccaagcttcc aattgaacta ttggatttgg ttcgtatcat
    18841 gagcgtttta ttgaataatg ctgtcgaagg atcggctgat agctataaaa agcagatgga
    18901 agtagcagtt attaagatgg aaactgaaac agttattgtg attcagaatt catgtaaaat
    18961 gacgatgact ccttcaggag atctatttgc cttaggattc tccactaagg gaagaaatcg
    19021 cggagtcgga ttaaataatg tgaaagaact actagataag tacaacaata ttattttaga
    19081 aacagagatg gaaggcagta catttagaca aatcattaga tttaagaggg aatttgaatg
    19141 aaagttttaa ttttagaaga tgttattgaa catcaagtga gactagagag aatattggat
    19201 gaaatttcga aagaatcgaa tattccaata tcatacaaga caacgggaaa agtccgtgaa
    19261 tttgaagaat acattgaaaa tgatgaagta aatcagcttt atttcctaga tatcgatatt
    19321 catggaattg agaaaaaggg atttgaagtg gctcagctca ttcgtcatta caatccttac
    19381 gctattatcg tctttatcac tagtcgatca gagtttgcga ctctaaccta taaataccag
    19441 gtatcagccc tagattttgt tgataaggat atcaatgatg agatgtttaa gaagagaatt
    19501 gagcaaaata tcttctacac gaagagtatg ttacttgaaa atgaagatgt tgtagattat
    19561 ttcgactaca attacaaggg aaatgattta aaaattcctt accatgatat tttgtatatt
    19621 gaaacaacag gggtctctca taaattgcgc attattggta agaattttgc aaaagagttt
    19681 tatggtacca tgacagatat tcaggaaaag gacaaacata ctcagcgatt ttattctcct
    19741 cacaagtcat ttttggtaaa tataggcaat atcagagaaa ttgatcgaaa aaacttagaa
    19801 attgttttct atgaagacca tcgttgtcct atttcaagat taaaaattag aaaattaaaa
    19861 gatattttag agaaaaaatc tcaaaagtga ttgacaatta gcaagaaatt gatataatgg
    19921 ttatatctgc tacagataga aggtccgttg gtcaaggggt taagacaccg ccttttcacg
    19981 gcggtaacac gggttcgaat cccgtacgga ctattttatc cgccatagct cagttggtag
    20041 tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc ctactggcgg agtatagata
    20101 aaagggaaca cagctgtgtt cctctttttg tatcaatttg tatcaccaag cattttcata
    20161 aggaagtctg ttatttcttg agaactttct ttttttccat gtgcaatcca agtttggcag
    20221 acaccaaaaa gtgcatgagt tagatagatg ctactatatt ctaattcggt ggtatttaga
    20281 ttcagttgca taaatcgctt ttgtaaatct gtactaagca tgatatgaag tttatttcgt
    20341 aagaaatttt ggatttcttt agtcccattt tcagaaagaa gggcagccag aagtggttct
    20401 gactctagat attcaaaaac ttctaaaata gcgtctcttt tgtgatgagc atgtttttga
    20461 aaaatatatt caaatgtatg gaatagcttg ctttgatagt gctcaatcat atcatactta
    20521 tccttatagt gagtatagaa gctggaacga ctaattccgg ctttttctac taatttgaca
    20581 gtagaaattt tatcaaatgg ctgttccatc agtaattgta ccatagcatt ttcaatagtt
    20641 cgctttgttt ttaagcgttt gttactttct tgcatatttc ctccttgtaa acaaattaga
    20701 ctatatgtct aaaaatagat tttttatctt gtaatttaga ttttttaatg tataatctat
    20761 tatatcaaaa ttttagacaa tatgtttaaa aaaggagaaa ctaagtttaa agaatggaaa
    20821 gcaatttaaa aaaaaccaac ctttattatt gtcatgatcg ggatttctct tattccagat
    20881 ctgtacaata tcatattttt gtcatcaatg tgggatccat atgggcaatt gtctgactta
    20941 cctgtggcag ttgtaaataa tgataaagag gcttcctata atggtaatac tatggcaata
    21001 ggaaaagaca tggtgtccaa tttaaaagaa aataaaacct tggattttca ttttgtagat
    21061 gaagaggaag gaaagaaggg attggaagat ggcgattact atatggtagt gactttacca
    21121 agtgatttat ctgaaaaaac aactacatta tccaatattc aatcgacagc agcttatcaa
    21181 tcattgacaa gtgagcaaca aactgagata agtgattctg tatctcaaaa ttcaactgat
    21241 agtattcaat cggctcagtc aattgtagct ttagtacaag atttacaggg aagtttagaa
    21301 aacttacaaa atcaatcttc taatctttcg actttaaaaa atcaatctaa tcaagtatca
    21361 cctattactt ctacttcttt gataggattg tcaagtggat taacagagat acaaggagat
    21421 gttactagca aattagttcc tgccagtcag tcgattgcat caggtgtaaa cgcatatact
    21481 acaggtgttg ataaagtttc tcagggcgca agtcaactaa gtgaaaaaaa tgccaccttg
    21541 acaggtagtt tgaataaact agtttcaggc tcaaacacct tgacacaaaa atcttctaga
    21601 ttgacagcag gagttggtta attacaatca ggatctgggc aattagcaga caaatccagt
    21661 cagttacttt caggtgcttc tccattagag aatagagcta ataaattggc agatggatct
    21721 gggaaactag cagaaggtgg aacaaagtta acttctggat tggaagattt acagacagga
    21781 cttgcttctt taggacaagg actaggtaat gctagtgatc aactcaaatc agtatcaaca
    21841 gaatctaaaa atgcagagat tttgtcaaat ccactcaatc tttcaaaaac agacaatgat
    21901 caagttcctg taaatggaat cgcaatagct ccttatatga tatcagttgc tctttttttt
    21961 gcagcaatat caacaaatat gatatttgcg aaattgcctt caggacgtca tccagagagc
    22021 cgttgggctt ggttgaaatc ttgagctgaa ataaatggta ttatagctgt tttggcagga
    22081 attttggtat atggaggagt tcagcttatt ggtttaactg ctaatcatga gatgagaata
    22141 tttattctca tcatcctaac aagtttagta ttcatgtcta tggtgaccac tttagcaacg
    22201 tggaatagcc gtataggagc ttttttctca cttattttgc ttttactaca gttagcatca
    22261 agtgcaggta cttatccact tgctttgaca aatgatttct ttagatctat taatccctgg
    22321 ttaccaatga gctattcagt ttcgggatta cgacaaacaa tctctatcaa caagtcattt
    22381 tcctagctgt catactagct ctatttactg gtttaggtat gctagcctat caacataaga
    22441 aaatggaaga agattaaaaa aatcgaccga ttaactggtc gattttttat gccttagatg
    22501 actttcgtct gtgattatag attccaaata gtaagagaga agtaaaggaa cagattgctc
    22561 cagtaataaa accattggga atgaaggaaa gtgtaatagt tcctttcccc ttgggaatgt
    22621 caactttcat aaatccagtt tgagcttgtt taatttctat tttcttacca tcttggtagg
    22681 cagaccaacc tttgtcataa ggaatggtga agaaaataga tgtatcttgt tggacatcat
    22741 atgtagcaaa aaccttgttt ttagaagttg atactgtgac aggttgttct ttaatttttt
    22801 gaattgcctc ggtgaaagtt ttggtatcta aacgatagaa ggtaggagat tcaaatgata
    22861 cttgtgaatt tccagggaaa ctaacattga tattgaaagt ttttttctct ttagtatatc
    22921 ctagattaaa gaaggagaag acattatcag ttgtaaaagt ctttttttca ccatttacaa
    22981 ggatgtcaac cttcttttgt ttatcgttag aaaagtgaag gtttatgaaa gagagataaa
    23041 cttggctgtt ttctggaact tcaatttgat actggattgc tgcatcttca tttgaagaac
    23101 ttgtgacact aatcaaatca ttagtatttt ctattttttc tgttttttca taaggtattg
    23161 gagaaaaata atcaaaattg acgttagcaa gttgatttaa aaatgaggcc tgattatcca
    23221 aggtatgttc attgaacttg acatcattgt aaacagattg actagcaact gcaatcggaa
    23281 gagagtattg attttcatat agggtaagat tatctttttg atagatatct ttaaagccat
    23341 acttatcaat aggactgtct gagatattgt actggatacc aaataaacta tcagccaaaa
    23401 tactattatt tgcatatcgg agattgagat tagtcccaga ggatttaaaa ccaagtttat
    23461 ctaaagtaga gcttgatgaa cgatttcgaa cagatgaaaa ttgagagatt ccattgtagt
    23521 tgaatttcat actgtcattt cctgtctgag tttgtagttt ttcagtacga gtaaattgat
    23581 ttccaatata tgttgagaaa gattccatag ctgggatatc tcgactataa gcacttcgag
    23641 aagcaaatcc ccattcctta gcaattccgt ccatttgaga tgaagcattt aaactcattt
    23701 caaccagtat aaataaagag attagaatgg caaatagatt cacagatata aactttttga
    23761 taactgcaag gagtaaaaga gaatagacaa ccaaaaattc aagagtaagc agaatattca
    23821 aatctgttaa aaaagaataa tgcgatttta gatagatggt agctaaaaat cctgttacta
    23881 caagaaaaag cgaaactaaa aaattccaga ctttaagttc tttcagacgc tttaagactt
    23941 ctgctgctgt gtaaattaac aaggtagaga aaatccaagc atagcgatgt aaaaacatgt
    24001 ttggagtatg catgccttgc caaaataagt caagagcttc tatgtaaaag cttgcaatta
    24061 gaaatgcaaa gaatattata tatatgagtt tcacgtgaaa cttaatagat ttcagcgtaa
    24121 aaaataaaat ggtcaaaata aagggaagta gtccaacaaa aatcattggg atggccccat
    24181 actttgttgt gtcaaaggaa ccaatgaatt gcttagcaaa gagatcaaga taccagctac
    24241 tttcagtttg aaactttgta acttcagtca atttttcccc atgtgtctgt aaatcaaaca
    24301 gagtgggaag agtcataatc aaactagcca taccagctaa aaaggagata actatgaaat
    24361 caagaacaga tgattttcga gtcttaaagt cccacgaaat ttgacagaga taccagaaaa
    24421 taagaaacaa tactgtcata tatccaaaat aataattttg aataaataag attgacagac
    24481 ttgtaaagta caataggagt ttcttttcag ttatcagtag atgtaaacca gttataatta
    24541 aaggaatcaa gataaaaaca tctagccagg tttttatctc taattgactg acagtgaaac
    24601 tcatcagagc ataggaagta gataaggcta gctttaaagt ctgagggata gattgaaaca
    24661 atttattcaa actaaaaaag gttgacagac caatcaatcc aaattttaag agagtggtca
    24721 gatagacagc atctggcata ttcgttagat caaaaaagta aactagaggc gcgagaaaac
    24781 tacccaagta ataactagat agggcataga agtttagccc tagaccactt gtaaaggtgt
    24841 aaaacagact actatttcca tgtaggatat ttcgtaaggc tacatcaaaa ataacgtatt
    24901 gatgaaagcc atctcctaat agaggagagt tgtcgctatt ccagtagata ccttgagata
    24961 gatatactcc agacataatc actacaggaa tgatgaaaga aataaaatag gttcgatatg
    25021 tttttaaaaa tgatttcatg ttacctcgta gaatgataga aaactcagtt ggttaaccca
    25081 actgagtttt gaagttttat ttagtctttc caaagttctt taacttttgc ttgtacttct
    25141 gcattttcta ggaattcatc gtaggtttca tcgatacggt caatgacgcc atttttagat
    25201 aagacaatga tatggttagc caaagtttga ataaattcgt ggtcatggct agcaaagatg
    25261 attgattctt taaagttttt caatccatca ttcaagcttg agatagattc caagtccaag
    25321 tgatttgttg gatcatcaag tacaaggaca tttgatttta agagcatgag ttttgaaagc
    25381 atgacacgaa ctttttctcc ccctgacaag acatttacag gtttgttaac ttcatctcca
    25441 gagaagagca tacggccgag gaaaccacgt aggaaagtat tgtcatcttc ttctttactt
    25501 gcgaattgac gcaaccagtc aagaattgat tctcctcctg caaaatcagc tgagttatct
    25561 tttggtaggt aagattgact agttgtaact ccccacttga cagttccttc atagtcaata
    25621 tctcccatga ttgcacgaat taatgcagtt gtttgaatat cattttgtcc aataagtgct
    25681 gtcttatcat ctggacgcaa gatgaaacta atattatcca agatagtttc accatcaatc
    25741 tttacagtta gattttctac tgtcaagaga tcattaccaa tctcacgttc cgctttaaag
    25801 ttgataaatg gatatttacg actagatggt acaatctctt ctagctcaat cttatcaagc
    25861 attttcttac gtgatgttgc ctgccttgac ttagaagcat tggcagagaa acgagcaacg
    25921 aattcttgca attgtttaat tttttcttct gctttagcat tacggtctgc tagcaattta
    25981 gcagcaagct cagaagattc cttccagaag tcgtagtttc cgacatagag tttgattttt
    26041 ccaaagtcaa ggtcggccat gtgagtacaa actttgttta agaagtgacg gtcgtgggat
    26101 actacgataa ctgtgttatc aaagtcaatc aagaagtctt ctaaccaagt aatcgattgg
    26161 atatccaaac cgttagtagg ctcgtccaag agaagaacat ctggtttacc aaaaagtgct
    26221 ttggcgagga gaacctttac tttttcaccg ttggccaatt cgctcatgtt ttggtagtgt
    26281 aattcttctg gaatgtttag gttttgaagt agttgagagg cttcactctc tgcttcccaa
    26341 cctccaagtt cggcaaactc tccttcgagt tcggcagcac gaaccccgtc ctcgtctgag
    26401 aaatcttcct tcatgtagat agcatctttc tctttcatga tgctataaag tttttcattt
    26461 cccatgataa cgacatcaat ggcacgttca tcttcgtagt caaagtgatt ttgacgaaga
    26521 acagagagac gttcatctgg accaagagag atgtgaccag tagtaggttc gatatctcca
    26581 gctaaaattt ttaaaaaggt tgattttccg gcaccattag caccgattaa tccgtaagta
    26641 tttccttctg taaatttgat attgacatca tcaaaaagtt tgcgatcact aaaacgtagt
    26701 gaaacatcag atactgtaag caatattttt ctcctatatg tgtaatatat ttattctact
    26761 agaaaataca gaaatattca aatttttatt tgtcaatttt gtgtaaatta tatttacagt
    26821 atcctttaca caaatctgta aaaagcaagg ctgatttatt ttgataaatt acggttattt
    26881 cattaaaaaa atgctataat tgaaaggact atatcgaagg agaacaaaat gactaaaccc
    26941 attattttaa caggagaccg tccaacagga aaattgcata ttggacatta tgttggaagt
    27001 ctcaaaaatc gagtattatt acaggaagag gataagtatg atatgtttgt gttcttggct
    27061 gaccaacaag ccttgacaga tcatgccaaa gatcctcaaa ccattgtaga gtctatcgga
    27121 aatgtggctt tggattatct tgcagttgga ttggatccaa ataagtcaac tatttttatt
    27181 caaagccaga ttccagagtt ggctgagttg tctatgtatt atatgaatct agtttcgtta
    27241 gcacgtttgg agcgaaatcc aacagtcaag acagagattt ctcagaaagg atttggagaa
    27301 agcattccga caggattctt ggtctatcca atcgctcaag cagctgatat cacagctttc
    27361 aaggctaatt atgttcctgt tgggacagat cagaaaccaa tgattgagca aactcgtgaa
    27421 attgttcgtt cttttaacaa tgcatataac tgtgatgtct tggtagagcc ggaaggtatt
    27481 tatccagaaa atgagagagc agggcgtttg cctggtttag atggaaatgc taaaatgtct
    27541 aaatcactaa ataatggtat ttatttagct gatgatgcgg atactttgcg taaaaaagta
    27601 atgagtatgt atacagatcc agatcatatc cgcgttgagg atccaggtaa gattgaggga
    27661 aatatggttt tccattatct agatgttttt ggtcgtccag aagatgctca agaaattgct
    27721 gatatgaaag aacgttatca acgaggtggt cttggtgatg tgaagaccaa gcgttatcta
    27781 cttgaaatat tagaacgtga actgggtcct attcgtgagc gccgtattga atttgctaag
    27841 gatatgggag aagtttataa tatgattcaa aaaggtagtg aaagagcgcg tgaagttgcg
    27901 ggtcaaaccc tatctgaggt aaaaggggca atgggacttc attactttaa ctaagtttat
    27961 tttacaagaa actatcattt ctatctcgaa gaaaagatag ttttttattt acccctgtaa
    28021 catttgacaa atttttatag aatggtatga tagatacaat ataaaaagag tcaagctcaa
    28081 aaagaaagaa aagaggaaac ttcgaatgtc taattgggac actaaatttt tgaaaaaagg
    28141 ttttaccttt gatgatgtat tgcttattcc agctgaaagt catgtgttgc ctaacgatgc
    28201 agatttaaca actaaattgg cagataattt gactttaaat atcccaatta ttaccgctgc
    28261 catggacaca gttacagaga gtcaaatggc cattgctatt gctcgtgcag gtggtctcgg
    28321 agttatccat aaaaacatgt caattgctca acaagcagac gaggttcgta aggtaaaacg
    28381 ttctgaaaat ggagttatta ttgatccgtt cttcttgacg cctgaacata caattgctga
    28441 agcagatgag cttatgggtc gttaccgcat cagtggtgtt ccagttgttg aaacacttga
    28501 aaatcgtaaa ttggttggta ttttgacaaa ccgagatctt cgttttattt cagattataa
    28561 tcaaccaatt tcaaaccata tgactagtga aaatcttgtt actgctcctg tgggtacgga
    28621 tcttgcaacg gctgagagta ttcttcaaga gcatcgtatt gaaaaacttc cgttggtcga
    28681 tgaagaaggc agtctttctg gtttgatcac tatcaaagat attgaaaaag ttattgagtt
    28741 tccaaatgcg gctaaagatg agtttggtcg tcttctagtt gcaggtgcag taggtgttac
    28801 ttcagataca tttgaacgtg cagaggctct ttttgaggca ggagcggatg cgattgttat
    28861 tgatactgca catggtcatt ctgcaggtgt cttgcgtaaa attgccgaga ttcgtgctca
    28921 tttcccagat cggactttga ttgctggaaa tattgctact gctgaaggtg cacgtgccct
    28981 ttatgaagcg ggtgtagacg ttgttaaggt tggtattgga ccaggttcta tctgtactac
    29041 tcgtgtgatt gctggtgttg gtgttccgca agtaacagct atctacgatg ctgcagctgt
    29101 tgcgcgcgaa tatggtaaaa cgattattgc tgacggtggg atcaagtatt ctggagatat
    29161 tgtaaaagca cttgctgcag gtggaaatgc tgttatgctt ggatctatgt ttgctggaac
    29221 tgatgaagct ccaggcgaaa ctgaaatctt ccaaggacgt aaattcaaga cttaccgtgg
    29281 tatgggatca attgctgcta tgaagaaagg ttcaagcgac cgttatttcc aaggttctgt
    29341 caatgaagca aacaagcttg ttccagaagg aattgaaggt cgtgttgctt ataaaggagc
    29401 ggcagctgat attgttttcc aaatgattgg tggtattcgc tctggtatgg gttactgtgg
    29461 tgcagctaac cttaaagaac tacacgataa tgctcaattt attgaaatgt ctggtgctgg
    29521 tttgaaagaa agccatcctc atgatgtgca aattactaat gaggcaccaa attattctat
    29581 gtaaaaaaca atgaaaagaa ctccagtgaa aacaggagtt cttttacaat gttgtcaatt
    29641 tccatttaca gcagctttac catcctgaat agtgaagata cttagatttt ctggcagatt
    29701 ttgaagatgg tctaagcttg ttgttgtgat aaaggtttgg attgattgag aaatcgtttc
    29761 taataatttt aactgtctag tgttgtcaag ttcactcatc acatcgtcaa gcagtaatat
    29821 cggagattct gtggtaatgc tttccattaa ttcgatttct gctaatttta tcgagaggac
    29881 gagactacga tgttgacctt ggcttccgaa actagcatcc atcccattta tataaaaaga
    29941 aatgtcatct cgatgaggac cgacaccagt attcttttta aataaatctc tggatctact
    30001 tttttctaaa gcaattttga aagattcgga taagttttgt ttgtcagttg tattgacaga
    30061 agattgatag gatattgaca actcttcgat ctgattagag agttcaaaat gtttcttacg
    30121 cccaaatgat tctagttttt ttatgaaatc taagcggtga ttcattacac gacatccata
    30181 atcaactagc tgatcatcta acacagaaag gaatgtttca tctatttttt gaactgattt
    30241 taggtaagtg tttctttgct ttaggatgtg gttataattg gttaagtcag ataaatagat
    30301 tggcttaatt tgcccaagct ccatatcaat gaattttcgt cgaatcgaag gtgctccttt
    30361 aattagttgt aaatcttcag gagcaaataa gacaacattc atgtgtccta cataatctga
    30421 aaggcgtgcc tgttttaagt gattaacttt tgttacacgg cctttttgtg ttagttcgat
    30481 ttctagggga atggatccag tttttttctg aacgagacct gaaagatgaa gttgttcctc
    30541 atcaaaatga atgagatttt tatctgttcg agttcgatga ctacgcgtta aggctaaaaa
    30601 atagatagcc tctaacatgt ttgtttttcc ttgtgcatta cgtcccaaaa agacattcaa
    30661 tttaggatta aagtctattt tcgtttcttt gtaattacga aaagtcttga gagatagatg
    30721 ttgtagccac atgattatct accagggaat cgtggagctt gttggctttt aggtgatgaa
    30781 gcaggtttcg atttgtcttt tttaactccc ttattcatct ttttgacaag tttagcgatc
    30841 cgttcttttt caactttatc agcttggtat tcatcttgtt cttcagaagt aggttgtgtc
    30901 aacaagatgt caatgttcat gtcagggatg tcaactttat cgccaatacg aagttttttt
    30961 ccacgacgac tctctaattc cctattaaag taaacagaat gttcagagag aaatgattta
    31021 atagctcctc cgctatgtgt aattccaagt tctttgagta gtgcttggag ggtaataaat
    31081 tcttcaaata atttgtattc cataattcac ctcaatcttt ggtattatac catattttcc
    31141 taaaaatagt gagagagata gctagaaatg taagcgattt ttatttcaaa agtgttacag
    31201 ggaacaagaa aatttcaggt tttcgtgata taatagaagt ctgtatataa ggaggtaaat
    31261 catggagtta gtgcatggaa tttcaacaca ttttatccaa tcaaaaaagt ttaaaacaaa
    31321 caaaattacc gtgcgtttta ccgctccatt atcccttgat acgattgcag gtcacatgtt
    31381 gagtgcaagt atgctagaga ctgctaatca gatgtacccc acttctcaag atttgaggag
    31441 acacttggcc agtctatacg gtacagatat gtcaaccaat tgtttcagaa gagggcaaag
    31501 ccacattata gaattgacat ttacctatgt tcgtgatgag tttttaagta ggaaaaacgt
    31561 gctaacctct cagattttgg aacttgtaaa agaaactctt ttttcacccg cagtagttga
    31621 taatgggttt gatccggcct tatttgaaat tgagaaaaaa caattgctag caagtttagc
    31681 agctgatatg gatgattctt tttattttgc acataaagaa ttggataaat tgttttttca
    31741 tgatgaacgt cttcaattgg aatatagtga tttacgaaat cgtattttag ctgaaactcc
    31801 acaaagttct tattcttgtt tccaagaatt tttagccaat gatcgaatag atttcttttt
    31861 cctaggtgat tttaatgagg ttgaaattca aaatgtatta gaatcatttg gctttaaagg
    31921 tcgaaaagga gatgtgaagg ttcagtattg tcaaccttat tctaatatcc ttcaggaagg
    31981 tatggttcgg aaaaatgtgg gacaatccat tttggaatta ggttatcatt accgttctaa
    32041 atatggtgat gaacaacatt tacccatgat tgtaatgaat ggtttacttg gtggatttgc
    32101 tcactctaag ctctttacaa atgtccgtga aaatgctgga ttagcttata ccatttcaag
    32161 tgagcttgat ttatttagtg gattcttgag gatgtatgct ggtatcaatc gagaaaatcg
    32221 taaccaggct cgtaaaatga tgaataatca actgcttgat ttaaaaaaag gttattttac
    32281 agagtttgag ttaaatcaga ccaaggaaat gattcgttgg tcgttgttac tttctcaaga
    32341 taatcaatct tcattgattg aacgtgctta tcaaaatgcc ttatttggaa aatcttcagc
    32401 agactttaaa ggttggattg caaaactcga gcaagttgac aaagatgcta tttgtagagc
    32461 agctaataat gtgaaactac aggcgattta ctttatggaa ggaatagaat gacaaaggtt
    32521 gtttttgaaa aaaaatacta tccagctgta aaagaaatga tttatcgaac tcgtttggcc
    32581 aacggattga cagttgctct tttgcctaaa aaggaattta aagaggttta cgggagtgtc
    32641 actgtacagt ttggttcggt agatacgttt gtcacagaag ttgacggaga tgtaaaacaa
    32701 tatcctgcag gaattgctca ttttcttgaa cataaattat ttgagagaga agattctagt
    32761 gatttgatgt cggcttttac gagtctaggt gcagatagta atgcctttac aagctttaca
    32821 aaaacaaact atcttttttc agcaacggat tattttttag aaaatttata tttacttgat
    32881 gaattggtaa catcagcaca ctttactgaa gcttccattc tgacagagca ggatattatt
    32941 cagcaagaac gagaaatgta ccaagatgat ccagattcgt gtttattctt ttcaacttta
    33001 gcgaatttgt atcctggtac acctttagca actgatatag ttggaagtga ggagtccatt
    33061 tcccaaatca atctaactaa tttgcaagaa aattttacaa agttttacaa acctgtaaac
    33121 atgtctctgt ttttagttgg taattttgat gtggagcgag tacaggacta ttttgaaagc
    33181 aaagaactga aagattcaga ttttcaggaa gtagcaagag aaaagttgtt tttacagcct
    33241 gtaaagccaa cagatagtat gagaatggaa gtatcttctc ccaaactagc gattggagtt
    33301 agaggtaagc gagaagtttc tgaagcggat tgctatcgac atcatatttt attaaaatta
    33361 ttgtttgcaa tgatgtttag ttggacttcg gatcgttttc aaaaatgtta tgaatcaggt
    33421 aaaattgatg catccttatc tctggaagtt gaagtaacaa gtcgctttca ttttgtcatg
    33481 ttgacaatgg atacgaaaga gccagttgct ttgtctcatc aatttaggaa ggctattcgt
    33541 aattttacaa aggatttaga tattacagag gaacatttag atattatcaa aagagagatg
    33601 tttggcgaat ttttcagtag catgaactct cttgaattta ttgcaacgca atatgatgct
    33661 tttgaaaatg gtgagacaat ttttgatttg ccgaaaattt tacaggaaat tactttagag
    33721 gatgtccttg atgctggaca tcatttaata gatgatggtg acatagttga ttttacaata
    33781 ttcccatcgt agtaacctat tataatagac actagaaaga agggatgaca agtatgagaa
    33841 aaaaaacaat tggagaggtt ttacgattag ctagaatcaa tcagggattg agtttagatg
    33901 aattgcagaa aaagacagaa atccagttag atatgttgga agcaatggaa gcagacgatt
    33961 tcgatcaact tccaagtcct ttttacacgc gttctttctt gaaaaaatat gcatgggctg
    34021 ttgagttaga tgaccaaatt gttttggatg cttatgattc tgggagtatg attacttatg
    34081 aggaagtaga tgttgatgaa gatgagttga caggtcgtag acgttcaagt aagaaaaaga
    34141 agaaaaaaac atcattttta cctttatttt attttatcct ttttgcttta tcgattttaa
    34201 tttttgtgac ttattatgtt tggaactata ttcaaactca accagaggag ccttctcttt
    34261 ctaattacag tgtggttcaa tcaacaagtt caactagctc tgttccccac tcctcaagta
    34321 gtagttcttc tagtatagaa tcagctataa gtgtatcagg cgaaggaaat catgtagaaa
    34381 tcgcttataa gacaagtaag gaaacagtta aattgcaatt ggcagtttca gatgttacaa
    34441 gttgggtcag tgtttcagaa agcgaacttg agggcggtgt aaccttatcg ccaaagaaga
    34501 aaagtgcaga agcaacagtt gcaactaaaa gtcctgtaac aattacgtta ggtgttgtaa
    34561 aaggtgttga tttgacagta gataatcaga ctgttgattt atcgaaatta acagctcaga
    34621 ctggacaaat cactgtaacc tttactaaaa attaaggaaa aacgaatgaa aaaagaacaa
    34681 attcccaatc tcttaacaat aggtcgaatt ctctttatac ctatttttat ctttatttta
    34741 acgataggaa attcgataga gagtcatata gttgcagcta ttatctttgc tgttgccagt
    34801 attaccgact atttagatgg atatttagct cgtaaatgga atgtggtcag taattttggt
    34861 aaatttgcag atcctatggc ggataagtta ctagttatgt cggcttttat tatgttgatt
    34921 gagttaggta tggctccggc ttggattgtt gcagtgatta tctgtcgtga gttagctgtg
    34981 acaggtttaa ggcttttatt ggttgaaact ggtggaacaa ttttagcagc agcaatgcct
    35041 ggaaaaatta aaacttttag tcagatgttt gccattattt tcttgctatt acattggact
    35101 ttgcttggtc aagttctact ttatgtagcc ttatttttca ctatctactc tggctatgac
    35161 tatttcaagg gtagtgccta tgtatttaaa gggacatttg gttcgaaatg aaatcaataa
    35221 ttgatgtaaa aaatctttct tttcgctata aagaaaatca gaactactac gatgtgaagg
    35281 atattacgtt tcacgtgaaa cgtggagaat ggctttcgat tgtagggcat aatggtagtg
    35341 gtaaatcaac gacggttcga ttaattgatg gcttactgga agcagaatcc ggagagattg
    35401 taattgatgg ccaacgtttg actgaggaaa atgtttggaa tatacgtcgt caaatcggta
    35461 tggtttttca aaatccagac aatcaatttg ttggagcgac tgttgaagat gatgttgctt
    35521 ttggtttgga aaatcaggga ctttctcgtc aagaaatgaa aaagagagtg gaagaagctc
    35581 tggctttagt tggcatgttg gactttaaaa agagagagcc agcgcgtcta tcaggtggtc
    35641 aaaagcaacg tgtggccatt gcaggtgttg tagccctaag accagctatt ttaatcttag
    35701 atgaagcaac gagtatgttg gatcctgagg ggcgtagaga acttattggg acagtaaaag
    35761 gaattcgaaa agactatgat atgacggtca tttctattac ccatgatttg gaagaagtcg
    35821 ccatgagtga tcgcgtattg gtcatgaaaa aaggggaaat tgaatcaact agtagtccaa
    35881 gggagctttt ctctcgaaat gatttagatc aaattggatt agacgatcct tttgctaatc
    35941 aattaaaaaa atctttgagc cagaatggct atgatttacc tgaaaattat ttgacagaaa
    36001 gtgagctaga ggataagcta tgggaattgc tctagaaaat gtgaatttta catatcaaga
    36061 aggtactccc ttagcttcag cagctttgtc ggatgtttct ttgacgattg aagatggctc
    36121 ttatacagct ttaattgggc acacaggtag tggtaaatca actattttac aactcttaaa
    36181 tggtttattg gtgccaagtc aagggagtgt gagggttttt gataccttaa tcacctcgac
    36241 ttctaaaaat aaagatattc gtcaaattag aaaacaggtt ggcttggtat ttcagtttgc
    36301 tgaaaatcag atttttgaag aaacggtttt gaaggacgtt gcttttggac cgcaaaattt
    36361 tggagtttct gaagaagatg ctgtgaagac tgcgcgtgag aaactggctc tggttggaat
    36421 tgatgaatca ctttttgatc gtagtccgtt tgagctgtca gggggacaaa tgagacgtgt
    36481 tgccattgca ggcatacttg ccatggagcc agctatatta gtcttagatg agccaacagc
    36541 tggtctagat cctctaggga gaaaagagtt gatgaccctg ttcaaaaaac tccaccagtc
    36601 agggatgacc atcgtcttgg taacgcattt gatggatgat gttgctgaat atgcgaatca
    36661 agtctatgta atggaaaagg gacgtttagt aaaggggggc aaaccaagtg atgtctttca
    36721 agacgttgtt tttatggaag aagttcagtt gggagtacct aaaattacgg ccttttgtaa
    36781 acgattggct gatagaggcg tgtcatttaa acgattaccg attaagatag aggagttcaa
    36841 ggagtcgcta aatggatagt atgattttgg ggcgttatat cccaggggat tcgattgttc
    36901 accgattgga tccacgtagc aaattgcttg ctatgatgct actgattttg attgtgtttt
    36961 gggctaataa ccccttgacg aatctgattc tttttatagc gacagggata tttattgcct
    37021 tgtcaggagt atctctttca ttttttattc agggcttgaa gtctatgttt ttcttgattg
    37081 ccttcacaac tatttttcaa ctatttttca tttctaatgg gaatgtttta tttgagtttt
    37141 cgtttgtgag aatcacggat tatgctttgc aacaagctgg gattattttt tgtcgctttg
    37201 tattgattat tttcttttca actttgttaa ccttaacaac catgccttta agtttggcat
    37261 cagctgtcga agctttatta gcacctttaa agcgtgtgaa agttccagtt catgaaattg
    37321 gattgatgct gtctatgagt ttgcgttttg tcccaacctt gatggatgat acgacgcgga
    37381 ttatgaatgc acagaaagct cgtggagtgg attttggaga aggaagcatc gttcaaaaag
    37441 taaaggcgat gattcccatt ttgattcctc tttttgcgac aagtttaaaa cgtgcagatt
    37501 ccttggctat cgctatggaa gcgcgtggct atcagggtgg aaaaggcaga agtcaatacc
    37561 gacaattgaa atggactcta aaggatacgt tgaccattct tgttattctc gtacttggtt
    37621 gttgtttatt tttcttaaaa tcttagtagc tttcaggaat tgataaaaag ttactgtaac
    37681 agtttttgat ataaaggtag gaatatgaac cgttttaaaa aatcaaaata tgtcattatt
    37741 gtttttgtca ctgttctgct tgtgtcagct ctcttagcga cgacttattc aagtacaatt
    37801 gtgacaaaat taggagatgg aatctcattg gttgatagag ttgtacaaaa accttttcag
    37861 tggtttgatt ctgtcaaatc agatttggct catttgacac gaacatataa tgaaaatgaa
    37921 agtttgaaga aacagcttta ccaattagaa gttaaatcaa atgaggtgga aagtttaaag
    37981 acagaaaatg aacaactgcg ccaattgctt gatatgaagt ctaaattgca agccacaaag
    38041 actttagcag cagatgttat tatgcgttct ccggtatctt ggaagcagga gttgacctta
    38101 gatgcaggta gatcaaaagg tgcttctgag aacatgttag ctattgcaaa tggtggcttg
    38161 attgggagtg tttcaaaagt agaggagaac tctactatag tcaaccttct gacaaatacg
    38221 gaaaatgctg ataagatttc tgttaaaatt caacatggct ctactacaat ttatggaatt
    38281 attattggct atgacaagga aaatgacgtt cttaaaatta gccaattaaa tagtaatagc
    38341 gatattagtg cgggagataa ggtgactact ggtggattag gaaactttaa cgttgctgat
    38401 attcctgttg gtgaagtggt tgccacaacg catagtacag actatttgac acgagaagta
    38461 actgttaaat tgagtgcaga tactcataat gtagatgtga tagaattagt ggggaattca
    38521 taatgagaca gttgaagcga gttggagtat ttttattgct tcctttcttt gttctaattg
    38581 acgcccatat tagccagctt ctgggctcat ttttccccca tgtacatttg gctagtcatt
    38641 ttctttttct atttctctta tttgagacga tagaagtatc agagtatctc tacctagtct
    38701 attgttttgt tataggcttg gtttatgatg tttacttttt ccatctaata gggattacaa
    38761 ctctcttatt tatcttattg ggagccttcc ttcataaatt gaatagtgtt attttgttga
    38821 atcgttggac aagaatgcta gctatgattg tgctgacatt cctgtttgaa atgggtagtt
    38881 atcttttggc ttttatggta gggttgacag tagatagcat gtcgattttt atagtctata
    38941 gcttggtacc gacgatgatt ttaaattttt tatggattac tgtttttcaa tttatttttg
    39001 aaaaatatta tctataagaa cgacatataa atgtaacaaa ggcgtaatat ttattaggcc
    39061 tttttttggt atactagtat tgtctttaaa agaaggagta tctacgtaat atgaagaaaa
    39121 aaatcttagc gtcactttta ttaagtacag taatggtttc tcaagtagct gttttaacaa
    39181 ctgcgcatgc agaaacgact gatgacaaaa ttgctgctca agataataaa attagtaact
    39241 taacagcaca acaacaagaa gcccaaaaac aagttgacca aattcaggag caagtatcag
    39301 ctattcaagc tgagcagtct aacttgcaag ctgaaaatga tagattacaa gcagaatcta
    39361 agaaactcga gggtgagatt acagaacttt ctaaaaacat tgtttctcgt aaccaatcgt
    39421 tggaaaaaca agctcgtagt gctcaaacaa atggagccgt aactagctat atcaatacca
    39481 ttgtaaactc aaaatcaatt acagaagcta tttcacgtgt tgctgcaatg agtgaaatcg
    39541 tatctgcaaa caacaaaatg ttagaacaac aaaaggcaga taaaaaagct atttctgaaa
    39601 aacaagtagc aaataatgat gctatcaata ctgtaattgc taatcaacaa aaattggctg
    39661 atgatgctca agcattgact acgaaacagg cagaactaaa agctgctgaa ttaagtcttg
    39721 ctgctgagaa agcgacagct gaaggggaaa aagcaagtct attagagcaa aaagcagcag
    39781 ctgaggcaga ggctcgtgca gctgcggtag cagaagcagc ttataaagaa aaacgagcta
    39841 gccaacaaca atcagtactt gcttcagcaa acactaactt aacagctcaa gtgcaagcag
    39901 tatctgaatc tgcagcagca cctgtccgtg caaaagttcg tccaacatac agtacaaacg
    39961 cttcaagtta tccaattgga gaatgtacat ggggagtaaa aacattggca ccttgggctg
    40021 gagactactg gggtaatgga gcacagtggg ctacaagtgc agcagcagca ggtttccgta
    40081 caggttcaac acctcaagtt ggagcaattg catgttggaa tgatggtgga tatggtcacg
    40141 tagcggttgt tacagctgtt gaatcaacaa cacgtatcca agtatcagaa tcaaattatg
    40201 caggtaatcg tacaattgga aatcaccgtg gatggttcaa tccaacaaca acttctgaag
    40261 gttttgttac atatatttat gcagattaat ttacagaggg actcgaatag agccctcttt
    40321 tcaggtttta ccgtgacaat ccctattaaa aattatatca aaatagcttg aaaatattgg
    40381 aaaagtatgg taaaatgaaa attgtcgtgt gaacgataat actcattctt gatgaattgt
    40441 gaagcagttg cccttgggtc gttttgcgag ttgaagtcaa gaagaggaaa aaaacaaaaa
    40501 ggagaaatac tcatggcagt aatttcaatg aaacaacttc ttgaggctgg tgtacacttt
    40561 ggtcaccaaa ctcgtcgctg gaatcctaag atggctaagt acatctttac tgaacgtaac
    40621 ggaatccacg ttatcgactt gcaacaaact gtaaaatacg ctgaccaagc atacgacttc
    40681 atgcgtgatg cagcagctaa cgatgcagtt gtattgttcg ttggtactaa gaaacaagca
    40741 gctgatgcag ttgctgaaga agcagtacgt tcaggtcaat acttcatcaa ccaccgttgg
    40801 ttgggtggaa ctcttacaaa ctggggaaca atccaaaaac gtatcgctcg tttgaaagaa
    40861 attaaacgta tggaagaaga tggaactttc gaagttcttc ctaagaaaga agttgcactt
    40921 cttaacaaac aacgtgcgcg tcttgaaaaa ttcttgggtg gtatcgaaga tatgcctcgt
    40981 atcccagatg tgatgtacgt agttgaccca cataaagagc aaatcgctgt taaagaagct
    41041 aaaaaattgg gaatcccagt tgtagcgatg gttgacacca atactgatcc agatgatatc
    41101 gatgtaatca tcccagctaa cgatgacgct atccgtgctg ttaaattgat cacagctaaa
    41161 ttggctgacg ctattatcga aggacgtcaa ggtgaggatg cagtagcagt tgaagcagaa
    41221 tttgcagctt cagaaactca agcagattca attgaagaaa tcgttgaagt tgtagaaggt
    41281 gacaacgctt aatttataca aatagtaatt acctaggagg gcggggctta gcccggctct
    41341 cctattttca aaaaatatag gagaattaaa atggcagaaa ttacagctaa acttgtaaaa
    41401 gagttgcgtg aaaaatctgg tgccggtgtt atggacgcta aaaaagcgct tgtagaaaca
    41461 gacggtgaca tcgaaaaagc gattgaattg cttcgtgaaa aaggtatggc taaggcagct
    41521 aagaaagctg accgtgttgc tgcagaaggt ttgactggtg tttatgttaa cggtaatgtt
    41581 gcagcagtta ttgaagtaaa cgctgaaact gacttcgttg caaaaaacgc tcaattcgtt
    41641 gaattggtaa atactacagc taaagtcatt gctgaaggaa aacctgctaa taatgaagaa
    41701 gctcttgctt tgataatgcc ttcaggtgaa actcttgaag ctgcatacgt atctgcaaca
    41761 gcaactatcg gagagaaaat ctcattccgt cgctttgcat tgattgaaaa aacagacgca
    41821 caacactttg gagcatacca acataacggt ggacgtatcg gtgttatttc agttgttgaa
    41881 ggtggagacg aagcacttgc taaacaattg tcaatgcaca tcgcagcgat gaaaccaaca
    41941 gttctttctt acaaagaatt ggatgagcaa ttcgttaaag atgagttggc acaattgaat
    42001 cacgttatcg accaagacaa cgaaagccgt gcaatggtta ataaaccagc tcttccacac
    42061 ttgaagtatg gatcaaaagc tcaattaact gatgatgtta ttgctcaagc tgaagctgac
    42121 atcaaagctg aattggctgc agaaggcaaa ccagaaaaaa tctgggacaa aattatccca
    42181 ggtaaaatgg atcgcttcat gcttgataat actaaagttg accaagctta cacacttctt
    42241 gcacaagttt acatcatgga tgacagcaag acagttgaag cataccttga atcagttaac
    42301 gcttcggtag ttgagtttgc tcgctttgaa gttggtgaag gtatcgagaa agctgcaaac
    42361 gacttcgaag ctgaagttgc agctacaatg gcagcagcct tgaataacta ataatagaaa
    42421 ggaagcaatt tgcttccttt tttgtatagg gcttatattt ttcctgaaat tgtttttccc
    42481 ccagtctcac tcgattagaa taattaaaac aaaaagccct ataataaggg ctaagtgcat
    42541 ttaggagact atacttcaaa ttcatagtaa gcgcgtcata acaaggtatc tatcctaatt
    42601 ccccaacttc cagtatacta gataaaaaat aattatcgga ggaaaggtat tgtactatga
    42661 tgattcaact aagtgattta ggtcaagttc acattgtttg tggcaagaca gatatgaggc
    42721 agggaataga ctcattagcc tatgtagtta aaacccactt tgaattggat tctttctccg
    42781 gtcaagtctt tctcttttgt ggtggacgta aagaccgctt taaagtcctt tactgggatg
    42841 gtcaaggatt ttggctacta tataaacgct ttgagaacgg aaaactgact tggctaagta
    42901 cagaaaagga tgtcaaagct ctcacatctg aacaagtaga ctggcttatg aagggctttt
    42961 ctatcactcc ccaaatatag tggatttaaa ctagaatagt acacctctgc ttctaaaaca
    43021 ttgttagaaa tcgatttgac tatcctgttc ttatttcatt ttactataaa ttcatcagaa
    43081 agtcgtgatt tctattgaaa tgaggacttt ctttttatac tcatctgttt tcaaaaagca
    43141 ttctagtccg tctccgatta acgatggact ttatcacctc cttctccagt ccttgtatga
    43201 catcttccaa agttcgaaag gctttattct taaatccacg tttacgaatc tctttccaca
    43261 cttgttcaat ggggttcatc tctggtgtgt atggaggaat aaatgcaaag ccaatattag
    43321 tcggaatctt taaggtactt gatttatgcc atatagcatt gtccataacg agtaaaagat
    43381 aatcatctgg ataagcttgt gaaagctcct attcctaaag cccctttagc gcataacttt
    43441 ggctcagctt ctattatcgc tcacaccatc catcagaagt ttaatctgaa ggtacccaat
    43501 tatcgccaag aagaagattg ggctaggatg ggtttaccaa tcacacgtaa ggaaatctct
    43561 aattggcata tcaagacgag tcaatactat ttggagcccc tttataacct cttgcgagag
    43621 agactattga ctcagccctt acttcatgcg gatgaaactt cttatagggt gctagagagt
    43681 gatagtcagc tgacttacta ttggactttt ttgtctggga aagctgagaa acaagggatt
    43741 acgctttacc atcatgttct aatcgatttg ttcatatctt atttcaatcc attataaata
    43801 gtgagaaata tctatcctat cttctagaat gtcttccaaa cgtggaaact ctcgtaaaca
    43861 aagaggtttt agaggcctat ttaccatgga ctaaagttgt acaagaaaag tgcaaataag
    43921 aaatctccag attaggaact atccgtgagt tctctagtct ggagattttt caatagactt
    43981 cgttattgga cggttacaat tcataaagtg ctgtagagag ataacgttca ccgttatctg
    44041 gtgctagggc aaggactttt ttacctgtac ctaatttttt ggcaacctcg atggctccgt
    44101 agatagctgc agctgaggaa atccctacaa ggaagccttc ttttccacca atttcacgtc
    44161 cgagtgcaag agcgtcatct gatgttacac gaacgatacc atcataggct ttagtatcaa
    44221 gtgtatcagg aataaatcca gctgagatac cttgaatttt gtgaggacca ggtttttcac
    44281 cagatagaat agcagattca tctgcttcta ctgcaaaaac ttgaatgtta gaattttctg
    44341 atttgagtgc atgagaaaca ccagaaatcg ttccaccagt acctactcca gcaacaaagg
    44401 catctaatcc atctttaccg aaagcagcta gtatctcagc tcctgttgtt ctttcgtgta
    44461 cttctggatt agctggattg tcaaattgaa gaggaaggaa accatcacgt tcagcagcga
    44521 tttcttgagc cttagcaata gcacctttca ttccctcgct accaggagtt aggacgagtt
    44581 cagcaccata agcttggata attttacgtc gttctacact catagtttca ggcataacga
    44641 tgacgacttt atacccttta gcagcaccta cccatgaaag tccaataccg gtgtttccac
    44701 ttgttgcttc aacaatagta gaaccaggtt tcagaatacc atcttgttca gctttttcaa
    44761 tcatgctaag ggcaatacgg tcttttacag atgaaccagg attaaatgct tcaagcttta
    44821 tatagacgtc tgcagcacct tctggcacga tgttgttaag tttaacaatc ggtgtttgac
    44881 cgattaattc agtaatgttg ttataaatag acatatgaat acctctttgt ataagatatc
    44941 tttagtataa cgctatccat accatttcta tatatcatag atcaaagtca ttaattttca
    45001 agtgattgcg ttcagctaat tctagtaaat aggtgtgaag gaaaacgata tcaggtttcg
    45061 tttgaattgc ttcattttct agtatatttt ggtacgcctc tttttatttt gctcgctaaa
    45121 gtagttgtga gaatgtcaac gaaacgctgt attattcatt gtttacacta agtttacagg
    45181 aacttcaacc tctcgtaaac cttggtcagt taaagtgact tttccattaa aaaactccac
    45241 aagtgcagct ttaatagttt ctttttcttc tttatcaaca taaatcatcg tatcgacttg
    45301 atctgtaaag tttgtatcca gctccatgag accatgttct ttaaggaagt tactgtactc
    45361 ttggtactga gcataagaca tttgaatagc aatgccagcc tgttctttta tttcaataat
    45421 accaatttct ttgacagcta aggcgacact gccggcgtaa gcacgaatta gtcctccagc
    45481 gcctagttta ataccaccaa agtagcgtgt cacgaccaca cagacattgg tgagattgtg
    45541 attttctagt accccaagca tgggaacacc agcagtacca ctaggctcac catcatcact
    45601 tgtacgttta atttcactac gttctccaat aatgaaggca gagcagttat gtgtcgcttt
    45661 gtagtgttct tttttgatgg cagtaatgaa gtcacgagcc tcttcttcgc tataaacacg
    45721 cttggcatgg cagataaagc gagatttttt gatttcttct tggacttgac cgtcctcttt
    45781 aattgtccta aattccatga tttaattgta ctaaaaaatc atctaaagtg ctagttttta
    45841 cgaataaaga agtatgaaag taaatttaga ttatctcggt cgtttattta ctgagaatga
    45901 attaacagaa gaagaacgtc agttggcgga gaaacttcca gcaatgagaa aggagaaggg
    45961 gaaacttttc tgtcaacgct gtaatagtac tattctagaa gaatggtatt tgcccatcgg
    46021 tgcttactat tgtcgagagt gcttgctgat gaagcgagtc agaagtgatc aaactttata
    46081 ctattttccg caggaggatt ttccaaagca agatgttctc aaatggcgcg gccaattaac
    46141 tccttttcaa gagaaggtgt cagagggatt gcttcaagta gtagacaagc aaaagccaac
    46201 cttagttcat gcggtaacag gagctggaaa gacagaaatg atttatcaag tagtggctaa
    46261 agtgatcaat gcgggtggtg cagtgtgttt ggctagtcct cgcatagatg tttgtttgga
    46321 gctgtacaag cgcctgcaac aggatttttc ttgcgggata gctttgctac atggagaatc
    46381 ggaaccttat tttcgaacac cactagttgt tgcaacaacc catcagttat tgaagtttta
    46441 tcaagctttt gatttgctga tagtggatga agtagatgct tttccttatg ttgataatcc
    46501 catgctttac cacgctgtca agaatagtgt aaaggagaat ggattgagaa tctttttaac
    46561 agcgacttcg accaatgagt tagataaaaa ggtccgttta ggagaactaa aaagactgaa
    46621 tttaccgaga cggtttcatg gaaatccgtt gattattcca aaaccaattt ggttatcgga
    46681 ttttaatcgc tacttagaca agaatcgttt gtcaccaaag ttaaagtcct atattgagaa
    46741 gcagagaaag acagcttatc cgttactcat ttttgcttca gaaattaaga aaggggagca
    46801 gttagcagaa atcttacagg agcaatttcc aaatgagaaa attggctttg tatcttctgt
    46861 aacagaggat cgattagagc aagtacaagc ttttcgagat ggagaactga caatacttat
    46921 cagtacgaca atcttggagc gcggagttac cttcccttgt gtggatgttt tcgtagtaga
    46981 ggccaatcat cgtttgttta ccaagtctag tttgattcag attggtggac gagttggacg
    47041 aagcatggat agaccgacag gagatttgct tttcttccat gatgggttaa atgcttcaat
    47101 caagaaggcg attaaggaaa ttcagatgat gaataaggag gctggtctat gaagtgcttg
    47161 ttatgtgggc agactatgaa gactgtttta acttttagta gtctcttact tctgaggaat
    47221 gatgactctt gtctttgttc agactgtgat tctacttttg aaagaattgg ggaagagaac
    47281 tgtccaaatt gtatgaaaac agagttgtca acaaagtgtc aagattgtca attttggtgt
    47341 aaagagggag ttgaagtcag tcatagagcg atttttactt acaatcaagc tatgaaggat
    47401 tttttcagtc ggtataagtt tgatggagac ttcctgttaa gaaaagtttt cgcttcattt
    47461 ttaagtgagg agttgaaaaa gtacaaagag tatcaatttg ttgtaattcc cctaagtcct
    47521 gatagatatg ctaacagagg atttaatcag gttgagggct tggtagaggc agcaggcttt
    47581 gagtatctag atttattaga gaaaagagaa gagagagcca gttcttctaa aaatcgttca
    47641 gagcgcttgg ggacagaact tcctttcttt attaaaagtg gagtcactat tcctaaaaaa
    47701 atcctactta tagatgatat ctatactaca ggagcaacta taaatcgtgt taagaaactg
    47761 ttggaagaag ccggtgctaa ggatgtaaaa acattttccc ttgtaagatg aggaaaaaat
    47821 ttgcaaaaat agaatgaaag cgttataata aaatcataaa aacaaaaatg tttagaaaga
    47881 aggtactcat atgattaaat atagtatccg tggtgaaaac ctagaagtaa cagaagcaat
    47941 tcgtgattat gtagtttcta aactcgaaaa gatcgaaaag tacttccaac cagaacaaga
    48001 gttggatgcc cgaattaact taaaagttta tcgtgaaaaa acggctaaag tggaagtaac
    48061 gattccgctt ggatctatta ctctccgcgc agaagatgta tctcaagata tgtatggttc
    48121 aattgacctt gtaactgata aaattgaacg tcagattcgt aaaaataaaa caaaaatcga
    48181 gcgtaaaaat aaaaataagg tagcaactgg tcaattattt acagatgctt tggtggaaga
    48241 ttcaaatatt gtccagtcta aagttgttcg ttcaaaacaa attgatttaa aaccaatgga
    48301 tttggaagaa gcaattctac aaatggattt attggggcat gatttcttta tctatgtgga
    48361 tgttgaagat cagacaacca atgtgattta tcgtcgtgag gatggcgaaa ttggtttgtt
    48421 agaggttaaa gaatcttaat ttcaatatgt gtaaaggtag gtttactgaa ttgtaaacta
    48481 cctttttctt ataattgata gaattactga ttacactttt aaaaagtcgc tttttgaggg
    48541 gtattatggt ataatgggtg ccaagaggtt ttgaatgaaa aaattttatg taagtccaat
    48601 ttttcctatt ctagtaggat tgattgcgtt tggagtctta tccactttca ttatttttgt
    48661 taataataat ctgttgacgg ttttaatttt gtttcttttt gtaggaggct atgttttttt
    48721 atttaagaaa ctgagagtgc attatacaag gagtgatgta gaacagatac agtatgtaaa
    48781 ccaccaagcg gaagaaagtt tgacagctct attggaacag atgcctgtag gtgttatgaa
    48841 attgaattta tcttctggag aggttgagtg gtttaatccc tatgctgaat tgattttgac
    48901 caaggaagat ggtgattttg atttagaagc tgttcaaacg attatcaagg cttcagtagg
    48961 aaatccgtct acttatgcca agcttggtga gaagcgttat gctgttcata tggatgcttc
    49021 ttccggtgtt ttgtattttg tagatgtatc cagggaacaa gccataacag atgaattggt
    49081 aacaagtaga ccagtgattg ggattgtctc tgtggataat tatgatgatt tggaggatga
    49141 aacttctgag tcagatatta gtcaaatcaa tagttttgta gctaatttta tatcagagtt
    49201 ttcagaaaaa cacatgatgt tttctcgtcg ggtaagtatg gatcgatttt atctatttac
    49261 tgactacacg gtgcttgagg gcttgatgaa tgataaattt tctgttattg atgctttcag
    49321 agaagagtcg aaacagagac agttgccctt gaccttaagt atgggatttt cttatggcga
    49381 tggaaatcat gatgagatag ggaaagttgc tttgctcaat ttgaacttgg ctgaagtacg
    49441 tggtggcgac caggtggttg ttaaggaaaa cgacgaaacg aaaaatccag tttattttgg
    49501 tggtgggtct gctgcttcaa tcaagcgtac acggactcgt acgcgcgcta tgatgacagc
    49561 tatttcagat aagattcgga gtgtagatca ggtttttgta gtcggtcaca aaaatttaga
    49621 catggatgct ttgggctctg ctgtaggtat gcagttgttc gccagcaatg tgattgaaaa
    49681 tagctatgct ctttatgatg aagaacaaat gtctccagat attgaacgag ctgtttcatt
    49741 catagaaaaa gaaggagtta cgaagttgtt gtctgttaag gatgcaatgg ggatggtgac
    49801 caatcgttct ttgttgattc ttgtagacca ttcaaagaca gccttaacat tatcaaaaga
    49861 attttatgat ttatttaccc aaaccattgt tattgaccac catagaaggg atcaggattt
    49921 tccagataat gcggttatta cttatatcga aagtggtgca agtagtgcca gtgagttggt
    49981 aacggaattg attcagttcc agaattctaa gaaaaatcgt ttgagtcgta tgcaagcaag
    50041 tgttttgatg gctggtatga tgttggatac taaaaatttc acctcgcgag taactagtcg
    50101 gacatttgat gttgctagct atctcagaac gcgcggaagt gatagtattg ctatccagga
    50161 aatcgctgcg acagattttg aagaatatcg tgaggtcaat gaacttattt tacaggggcg
    50221 taaattaggt tcagatgtac taatagcaga ggctaaggac acgaaatgct atgatacagt
    50281 tgttattagt aaggcagcag atgccatgtt agccatgtca ggtattgaag cgagttttgt
    50341 tcttgcgaag aatacacaag gatttatctc tatctcagct cgaagtcgta gtaaactgaa
    50401 tgtacaacgg attatggaag agttaggcgg tggaggccac tttaatttgg cagcagctca
    50461 aattaaagat gtaaccttgt cagaagcagg tgaaaaactg acagaaattg tattaaatga
    50521 aataaaggaa aaggagaaag aagaatgaaa gtaatctttt tagcagatgt taaaggaaaa
    50581 ggtaaaaaag gcgaaattaa ggaagtacca acagggtatg cgcaaaactt tcttatcaaa
    50641 aagaatctag ccaaagaagc gactgctcaa gctgtaggtg aacttcgtgg taaacaaaaa
    50701 tcggaagaaa aagctcacgc tgagatgatt gcagaaggga aagcaattaa agcacaactt
    50761 gaagcagaag aaactgttgt agaatttgtt gaaaaagttg gtccagatgg tcgtaccttt
    50821 ggttctatta ccaataagaa gattgcagaa gaattacaaa agcaatttgg aattaagatt
    50881 gataaacgtc atattcaagt acaagctccg attcgagcgg ttggtttgat tgatgtgcca
    50941 gtgaaaatct atcaagatat cacaagtgta atcaatcttc gtgtgaaaga aggataagtt
    51001 tacaccttct tgacaagatt gtaaaaggaa gggaagtctg atggcagaag tagaagagtt
    51061 acgagtacaa cctcaagata tcttagctga gcaatccgtt ttaggggcta tctttattga
    51121 tgagagtaaa cttgtttttg tgcgagaata cattgagtct cgggactttt ttaagtatgc
    51181 ccatcgtttg attttccaag ccatggtcga tttatccgat cgtggtgatg ccatagatgc
    51241 aacaacggtt cgtactatcc ttgataatca aggtgattta cagaatattg gtggcttgtc
    51301 ttacttggtt gagattgtca attctgtgcc aacttctgct aatgcggagt attatgctaa
    51361 gattgttgca gaaaaagcaa tgctacgtcg tttaattgcc aagttgacag agtctgtcaa
    51421 ccaagcttac gaagcgtcac aaccagctga tgaaattatt gctcaggcag aaaaagggct
    51481 gattgatgtc agtgaaaatg caaatcgaag cgggtttaag aacattcgag atgtgttgaa
    51541 tctcaacttt ggaaatctgg aagctcgctc gcaacaaacg accgatatta caggtattgc
    51601 gacaggttat cgtgatttgg atcatatgac aacaggactt catgaggagg agttgattat
    51661 cttagcagct cgtccagcag ttggtaagac agcatttgcc ttgaatatcg ctcagaatat
    51721 tgggactaag ttggacaaaa cggttgctat tttttcactc gaaatgggtg cggaaagctt
    51781 ggtagaccgt atgttagctg cagaaggctt ggtggagtca cattctatcc gtacagggca
    51841 attgacagat gaggagtggc aaaaatatac tattgctcag ggtaatctag ctaacgccag
    51901 tatctatatc gatgatacgc caggtattcg gattacagag attcgttctc gttctcgtaa
    51961 attggctcaa gaaactggaa atcttggttt gattttgata gactatttgc aacttatcac
    52021 gggaactggt cgagaaaatc gtcaacaaga agtttctgaa atttctcgtc agttgaaaat
    52081 actagccaag gaattgaagg ttccagtaat cgctctgagt cagctttctc gtggtgtaga
    52141 acaacgtcag gacaagagac cggtcttgtc tgatattcgt gaatctgggt ctattgagca
    52201 ggacgctgat atcgtagctt ttctctatcg cgatgactac tatgaacgtg gtggtgaaga
    52261 agaggagggt atcccaaata ataaggtgga agttattatc gagaaaaacc gtagtggagc
    52321 tcgtggaaca gtggaattga ttttccaaaa agaatacaat aaattttcaa gtatctcaaa
    52381 gagggaggca taagatgtca gatgcattta cagatgtagc caagatgaaa aaaatcaaag
    52441 aagaaatcaa ggcacatgag ggacaagtcg tagaaatgac tttggagaat ggtcgtaagc
    52501 gccaaaaaaa tagattgggt aagctaattg aagtttatcc atccctattt attgtggagt
    52561 ttggggatgt ggaaggagat aaacaagtta atgtttacgt tgaatccttt acttactcag
    52621 atattcttac agaaaagaat ttgattcatt atcttgacta aagtgagaaa ttttctcact
    52681 ttttcttttt tctccgaata atttaggtga aggcaatcat cgctttatat tattttttaa
    52741 ggaggaagaa tgaaaatttt accgtttata gcaagaggaa caagttatta cttgaagatg
    52801 tcagttaaaa agcttgttcc ttttttagta gtaggattga tgctagcagc tggtgatagt
    52861 gtctatgcct attccggagg aaatggatcg attgcgcgtg gggatgatta tcctgcttat
    52921 tataaaaatg ggagccagga gattgatcag tggcgcatgt attctcgtca gtgtacttct
    52981 tttgtagcct ttcgtttgag taatgtcaat ggttttgaaa ttccggcagc ttatggaaat
    53041 gcgaatgaat ggggacatcg tgctcgtcgg gaaggttatc gtgtagataa tacaccgacg
    53101 attggttcca ttacttggtc tactgcagga acttatggtc atgttgcctg ggtgtcaaat
    53161 gtaatgggag atcagattga gattgaggaa tataactatg gttatacaga atcctataat
    53221 aaacgaatta taaaagcaaa cacgatgaca ggatttattc attttaaaga tttggatggt
    53281 ggcagtgttg ggaatagtca atcctcaact tcaacaggcg aaactcatta ttttaagacc
    53341 aagtctgcta ttaaaactga acccctagtt agtgcaactg tgattgatta ctattatcct
    53401 ggagagaagg ttcattatga tcaaattctc gaaaaagacg gctacaagtg gttgagttat
    53461 actgcctata atggaagcta tcgttatgtt caattggagg ctgtgaataa aaatcctcta
    53521 ggtaattctg ttctttcttc aacaggtgga actcattatt ttaagaccaa gtctgctatc
    53581 aaaactgaac ccctagttag tgcaactgtg attgattact attatcctgg ggagaaggtt
    53641 cattatgatc agatacttga aaaagacggc tacaagtggt tgagttatac ggcttataac
    53701 ggaagtcgtc gctatataca gctagaggga gtgacttctt cacaaaatta tcagaatcaa
    53761 tcaggaaata tctctagcta tggatccaat aatagttcaa ctgtcggttg gaagaaaata
    53821 aatggtagtt ggtatcattt caaatcaaat ggttctaaat caacaggatg gctgaaagat
    53881 ggttctagct ggtattattt gaaattatct ggtgaaatgc agacaggatg gttaaaggag
    53941 aatggttcgt ggtattatct gggtagttca ggggcaatga aaacaggctg gtaccaggtc
    54001 tctggtaagt ggtattattc ttactcttca ggcgccttag ctgttaatac gacggtggat
    54061 ggctacagag taaacagtga tggagaacga gtatagaaaa ttggagtagg agaaatttcc
    54121 tgctcttttc tgtaagcagt ttccttgaca tttttcttat tttgcgctat catatataca
    54181 tataatatat gggagtctgt gtacagtctg agaggaagtg ttaaacttcg accgcacctg
    54241 atctgggtaa tgccagcgga gggaacgata cttagtctaa ttttgcacct tttccatgta
    54301 tggtaaaggt ttttcttttt tttaaaagga aaacgagaag aggaggttct tatgaaagca
    54361 agcattgcct tgcaagtttt acccctagta caggggattg atcggatagc tgttattgat
    54421 caggtcattg cttatctgca aactcaagaa gtgccgatgg tagtgacacc atttgaaacg
    54481 gtcttggaag gggagtttga tgagcttatg cgcattctaa aagaagcgct ggaagtggca
    54541 gggcaggagg cagacaatgt ctttgccaat gtcaaaataa atgtaggaga gattttaagt
    54601 attgatgaga aacttgagaa gtatactgag acgacacatt agtctattgg gctttctcgg
    54661 agtcttgtca atctggcagt tagcaggttt tcttaaactg ctccccaagt ttatcctgcc
    54721 gacacctctt gaaattctcc agccctttgt tcgtgacaga gaatttctct ggcaccatag
    54781 ctgggcgacc ttgagagtgg ctttactggg gctgattttg ggagttttga ttgcctgtct
    54841 tatggctgta ctcatggata gtttgacttg gctcaatgac ctgatttacc ctatgatggt
    54901 ggtcattcag accattccga ccattgccat agctcctatc ctggtcttgt ggctaggtta
    54961 tgggattttg cccaagattg tcttgattat cttaacgaca acctttccca tcatcgtcag
    55021 cattttggac ggttttaggc attgcgacaa ggatatgctg accttgttta gtctgatgcg
    55081 ggccaagcct tggcaaatcc tgtggcattt taaaattcca gtcagcctgc cctactttta
    55141 tgctggtctg agggtcagtg tctcctacgc ttttatcaca acagtggtat ctgagtggtt
    55201 gggaggcttt gaggggcttg gtgtttatat gattcagtcc aagaaattgt ttcagtatga
    55261 taccatgttc gctattatta ttctggtatc gattatcagc cttttgggta tgaagttggt
    55321 cgatattagt gaaaaatatg ttattaaatg gaaacgttcg tagaattaga atgtttctga
    55381 aaaagaaaag aggaaatcaa aatgaagaaa acatggaaag tgtttttaac gcttgtaaca
    55441 gctcttgtag ctgttgtgct tgtggcctgt ggtcaaggaa ctgcttctaa agacaacaaa
    55501 gaggcagaac ttaagaaggt tgactttatc ctagactgga caccaaatac caaccacaca
    55561 gggctttatg ttgccaagga aaaaggttat ttcaaagaag ctggagtgga tgttgatttg
    55621 aaattgccac cagaagaaag ttcttctgac ttggttatca acggaaaggc accatttgca
    55681 gtgtatttcc aagactacat ggctaagaaa ttggaaaaag gagcaggaat cactgccgtt
    55741 gcagctattg ttgaacacaa tacatcagga atcatctctc gtaaatctga taatgtaagc
    55801 agtccaaaag acttggttgg taagaaatat gggacatgga atgacccaac tgaacttgct
    55861 atgttgaaaa ccttggtaga atctcaaggt ggagactttg agaaggttga aaaagtacca
    55921 aataacgact caaactcaat cacaccgatt gccaatggcg tctttgatac tgcttggatt
    55981 tactacggtt gggatggtat ccttgctaaa tctcaaggtg tagatgctaa cttcatgtac
    56041 ttgaaagact atgtcaagga gtttgactac tattcaccag ttatcatcgc aaataacgac
    56101 tatctgaaag ataacaaaga agaagctagc aaagtcatcc aagccatcaa aaaaggctac
    56161 caatatgcca tggaacatcc agaagaagct gcagatattc tcatcaagaa tgcacctgaa
    56221 ctcaaggaaa aacgtgactt tgtcatcgaa tctcaaaaat acttgtcaaa agaatacgca
    56281 agcgacaagg aaaaatgggg tcaatttgac gcagctcgct ggaatgcttt ctacaaatgg
    56341 gataaagaaa atggtatcct taaagaagac ttgacagaca aaggcttcac caacgaattt
    56401 gtgaaataat gacagaaatt agactagagc acgtcagtta tgcctatggt caggagagga
    56461 ttttagagga tatcaaccta caggtgactt caggcgaagt ggtttccatc ctaggcccaa
    56521 gtggtgttgg aaagaccacc ctctttaatc taatcgctgg gattttagaa gttcagtcag
    56581 ggagaattgt ccttgatggt gaagaaaatc ccaaggggcg cgtgagttat atgttgcaaa
    56641 aggatctgct cttggagcac aagacggtgc ttggaaatat cattctgccc ctcttgattc
    56701 aaaaggtgga taaggcagaa gctatttccc gagcggataa aattcttgcg accttccagc
    56761 tgacagctgt aagagacaag tatcctcatg aacttagcgg tgggatgcgc cagcgtgtag
    56821 ccttactccg gacctacctt tttgggcaca agctctttct cttagatgag gcctttagcg
    56881 ccttggatga gatgacaaag atggaactcc acgcttggta tcttgagatt cacaaacagt
    56941 tgcagctaac aaccctgatc atcacgcata gtattgagga ggccctcaat ctcagcgacc
    57001 gcatctatat cttgaaaaat cgccctgggc agattgtttc agaaattaaa ctagattggt
    57061 ctgaagatga ggacaaggaa gtccaaaaga ttgcctacaa acgtcaaatc ttggcagaat
    57121 taggcttaga taagtagaaa aatagggagt tggtgaagat tatcctttac cagcgccctt
    57181 tttcttttaa aaatgagaaa atttcggtat aatagtcaaa caaggtcaag gtttaaagag
    57241 agaggtgggt ttgttatgag atttaaaaat acatcggatc atattgaggc ctacatcaag
    57301 gcgattttag atcaatctgg tatcgtggag ttgcaacgga gtcagttggc agataccttt
    57361 caggttgttc ctagtcagat taactacgtg atcaagacac gctttacgga aagtagaggc
    57421 tacttggttg aaagtaagcg tggtggtgga ggctacattc gtataggacg aattgagttt
    57481 tctagtcatc atgaaatgct ccgggagctg ctttactcga ttggtgagcg agtcagtcaa
    57541 gaaatttatg aggatattct ccagcttttg gttgagcagg aattgatgac caagcaggag
    57601 atgaatttgc tagaatcagt agctttggat cgcgttttag gagaagaagc tccagttgtt
    57661 cgagcaaaca tgctacgtca gatcatacaa gaggtagata gaaaagggaa gtaagatgaa
    57721 ctattcaaaa gcattgaatg aatgtatcga aagtgcctac atggttgctg gacattttgg
    57781 agctcgttat ctagagtctt ggcacttgtt gattgccatg tctaatcaca gttatagtgt
    57841 agcaggggca actttaaatg attatccgta tgagatggac cgtttagaag aagtggcttt
    57901 ggaactgact gaaacggact atagccagga tgaaaccttt acggaattgc cgttctcccg
    57961 tcgtttgcag gttctttttg acgaagcaga gtatgtagcg tcagtggtct atgctaaggt
    58021 gctagggaca gagcacgtcc tctatgcgat tttgcatgat ggcaatgcct tggcgactcg
    58081 tatcttggag agggctggtt tttcttatga agacaagaaa gatcaggtca agattgctgc
    58141 tcttcgtcga aatttagaag aacgggcagg ctggactcgt gaagatctca aggctttacg
    58201 ccaacgccat cgtacagtag ctgacaagca aaattctatg gccaatatga tgggcatgcc
    58261 gcagactcct agtggtggtc tcgaggacta tacgcatgat ttgacagagc aagcgcgttc
    58321 tggcaagtta gaaccagtca tcggtcggga caaggaaatc tcacgtatga ttcaaatctt
    58381 gagccggaag actaagaaca accctgtctt ggttggggat gctggtgtcg ggaaaacagc
    58441 tctggcgctt ggtcttgccc agcgtattgc tagtggtgac gtgcctgcgg aaatggctaa
    58501 gatgcgcgtg ttagaacttg atttgatgaa tgtcgttgca gggacacgct tccgtggtga
    58561 ctttgaagaa cgcatgaata atatcatcaa ggatattgaa gaagatggcc aagtcatcct
    58621 ctttatcgat gaactccaca ccatcatggg ttctggtagc gggattgatt cgactctgga
    58681 tgcggccaat atcttgaaac cagccttggc gcgtggaact ttgagaacgg ttggtgccac
    58741 tactcaggaa gaatatcaaa aacatatcga aaaagatgcg gcactttctc gtcgtttcgc
    58801 taaagtgacg attgaagaac caagtgtggc agatagtatg actattttac aaggtttgaa
    58861 ggcgacttat gagaaacatc accgtgtaca aatcacagat gaagcggttg aaacagcggt
    58921 taagatggct catcgttatt taaccagtcg tcacttgcca gactctgcta tcgacctctt
    58981 ggacgaagca gcagcaacag tgcaaaataa ggcaaagcat gtaaaagcag acgattcaga
    59041 tttgagtcca gctgacaagg ccctgatgga tggcaagtgg aaacaggcag cccagctaat
    59101 cgcaaaagaa gaggaagtac ctgtctacaa agacttggtg acagagtctg atattttgac
    59161 caccttgagt cgcttgtcag gaatcccagt tcaaaagctg actcagactg acgctaagaa
    59221 atatctaaat cttgaagcag aactccataa acgggttatc ggtcaagatc aagctgtttc
    59281 aagtattagt cgtgcgattc gccgtaatca gtcaggaatt cgcagtcaca agcgtccgat
    59341 cggttccttt atgttcctag gacccacagg tgtcggtaag accgagttgg ccaaggcttt
    59401 ggcagaagtt ctctttgatg acgaatcagc ccttatccgc tttgatatga gtgagtatat
    59461 ggagaaattc gcagccagcc gtctaaatgg agctcctcca ggctatgtgg gttacgaaga
    59521 aggtggggag ttgacagaga aggttcgcaa taaaccctat tccgttctcc tctttgatga
    59581 ggtagagaag gctcacccag atatctttaa tgttctcttg caggttctgg atgacggtgt
    59641 cttgacagat agcaagggac gcaaggtcga tttttcaaat accattatca ttatgacatc
    59701 gaacctaggt gcgactgccc ttcgtgatga taagactgtt ggttttgggg ctaaggatat
    59761 tcgttttgac caggaaaata tggaaaaacg catgtttgaa gaactgaaaa aagcttatag
    59821 accggaattc atcaaccgta ttgatgagaa ggtggtcttc catagcctat ctagtgacca
    59881 tatgcaggaa gtggtgaaga ttatggtcaa gcctttagtg gcaagtttgg ctgaaaaagg
    59941 cattgacttg aaattacaag cttcagctct gaaattgtta gcaaatcaag gatatgaccc
    60001 agagatggga gctcgcccac ttcgcagaac cctgcaaaca gaagtggagg acaagttggc
    60061 agaacttctt cttaagggag atttagtggc aggcagcaca cttaagattg gtgtcaaagc
    60121 aggccagtta aaatttgata ttgcataaaa gaataaaagt atcagcatct gaccataagt
    60181 cacagtggag tgaaactcaa tgaaaatcaa aga
//
LOCUS       DBEROU010000006        59687 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.006, whole genome shotgun sequence.
ACCESSION   DBEROU010000006 DBEROU010000000
VERSION     DBEROU010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 59687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.006"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..456)
                     /gene="lytA"
                     /locus_tag="ACL8CL_000409"
     CDS             complement(<1..456)
                     /gene="lytA"
                     /locus_tag="ACL8CL_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HGS1329714.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYP"
     gene            complement(832..2202)
                     /locus_tag="ACL8CL_000410"
     CDS             complement(832..2202)
                     /locus_tag="ACL8CL_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGS1329715.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFLASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            complement(2509..3675)
                     /gene="recA"
                     /locus_tag="ACL8CL_000411"
     CDS             complement(2509..3675)
                     /gene="recA"
                     /locus_tag="ACL8CL_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HGS1329716.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            complement(3730..4986)
                     /locus_tag="ACL8CL_000412"
     CDS             complement(3730..4986)
                     /locus_tag="ACL8CL_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HGS1329717.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            complement(5071..6087)
                     /locus_tag="ACL8CL_000413"
     CDS             complement(5071..6087)
                     /locus_tag="ACL8CL_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HGS1329718.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            complement(6095..6613)
                     /locus_tag="ACL8CL_000414"
     CDS             complement(6095..6613)
                     /locus_tag="ACL8CL_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGS1329719.1"
                     /translation="MEYELLIREAEPKDAAELVAFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            complement(6603..7046)
                     /gene="tsaE"
                     /locus_tag="ACL8CL_000415"
     CDS             complement(6603..7046)
                     /gene="tsaE"
                     /locus_tag="ACL8CL_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HGS1329720.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            complement(7132..7752)
                     /locus_tag="ACL8CL_000416"
     CDS             complement(7132..7752)
                     /locus_tag="ACL8CL_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329721.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            complement(8135..8998)
                     /locus_tag="ACL8CL_000417"
     CDS             complement(8135..8998)
                     /locus_tag="ACL8CL_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1329722.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            9187..9357
                     /locus_tag="ACL8CL_000418"
     CDS             9187..9357
                     /locus_tag="ACL8CL_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhrA family quorum-sensing system peptide"
                     /protein_id="HGS1329723.1"
                     /translation="MKKNRGIQKLAILVLLGVFMFSNTIPYQQFIQKNRQLEIRVQSQ
                     KKSNGLDVGKAD"
     gene            9672..9968
                     /locus_tag="ACL8CL_000419"
     CDS             9672..9968
                     /locus_tag="ACL8CL_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGS1329724.1"
                     /translation="MLKFRKVVASSLAVLAFSLALPVLAVSHRGGEWTYGGHHDPNNW
                     GAFSNYYHGSQYHWAYVGSNGRNNQKTVYAGARSAAYAFVNTNFGEQVTFDAGW"
     gene            10071..12086
                     /locus_tag="ACL8CL_000420"
     CDS             10071..12086
                     /locus_tag="ACL8CL_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HGS1329725.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSHYYPNVVVRDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKNLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMHLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEVWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLTTSTEWHDY
                     SPNGNVLIVTPHYLERQNIPVDTTIKQKMNHLNVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            12088..12720
                     /locus_tag="ACL8CL_000421"
     CDS             12088..12720
                     /locus_tag="ACL8CL_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329726.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWEMADEVFTMDHLK"
     gene            12861..13139
                     /locus_tag="ACL8CL_000422"
     CDS             12861..13139
                     /locus_tag="ACL8CL_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComA"
                     /protein_id="HGS1329727.1"
                     /translation="MKKFDNYIIEKPCDSNSDKLQKILIIENLVDDILQFPLRINNSV
                     GEIFLLQPFQKKTIFIPCYFEEDIVKVKDDDKVEWNLLEFQKFRAFLA"
     gene            complement(13648..14061)
                     /gene="ndk"
                     /locus_tag="ACL8CL_000423"
     CDS             complement(13648..14061)
                     /gene="ndk"
                     /locus_tag="ACL8CL_000423"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HGS1329728.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYLPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEESLPGTI
                     RGDFAKAAGENEVIQNVVHGSDSEESAKREIALWF"
     gene            complement(14172..17849)
                     /gene="rpoC"
                     /locus_tag="ACL8CL_000424"
     CDS             complement(14172..17849)
                     /gene="rpoC"
                     /locus_tag="ACL8CL_000424"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HGS1329729.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMSYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            complement(17882..21493)
                     /gene="rpoB"
                     /locus_tag="ACL8CL_000425"
     CDS             complement(17882..21493)
                     /gene="rpoB"
                     /locus_tag="ACL8CL_000425"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGS1329730.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFIDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVVAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            21909..21979
                     /locus_tag="ACL8CL_000426"
     tRNA            21909..21979
                     /locus_tag="ACL8CL_000426"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21941..21943,aa:Cys,seq:gca)
     gene            complement(22177..23508)
                     /locus_tag="ACL8CL_000427"
     CDS             complement(22177..23508)
                     /locus_tag="ACL8CL_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HGS1329731.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTNTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPVIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFNVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKIQITEVEIEEETE"
     gene            complement(23799..24623)
                     /gene="endA"
                     /locus_tag="ACL8CL_000428"
     CDS             complement(23799..24623)
                     /gene="endA"
                     /locus_tag="ACL8CL_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HGS1329732.1"
                     /translation="MNKKTRQTLIGLLVLLLLSAGSYYIKQNELGNQAAKTKMSQKKQ
                     ASEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            complement(24662..24850)
                     /locus_tag="ACL8CL_000429"
     CDS             complement(24662..24850)
                     /locus_tag="ACL8CL_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HGS1329733.1"
                     /translation="MNKKSGYVIKRLLLVILVLILGLLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            complement(24843..26126)
                     /gene="murA"
                     /locus_tag="ACL8CL_000430"
     CDS             complement(24843..26126)
                     /gene="murA"
                     /locus_tag="ACL8CL_000430"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGS1329734.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            complement(26150..26380)
                     /locus_tag="ACL8CL_000431"
     CDS             complement(26150..26380)
                     /locus_tag="ACL8CL_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 family protein"
                     /protein_id="HGS1329735.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDDNTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            complement(26438..27475)
                     /locus_tag="ACL8CL_000432"
     CDS             complement(26438..27475)
                     /locus_tag="ACL8CL_000432"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepM family pheromone-processing serine
                     protease"
                     /protein_id="HGS1329736.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            complement(27459..27947)
                     /gene="coaD"
                     /locus_tag="ACL8CL_000433"
     CDS             complement(27459..27947)
                     /gene="coaD"
                     /locus_tag="ACL8CL_000433"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HGS1329737.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLCVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDELVVDVAKRLGATCLVRGLRNASDLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACYVPESILEEIRNEK
                     KD"
     gene            complement(27937..28476)
                     /gene="rsmD"
                     /locus_tag="ACL8CL_000434"
     CDS             complement(27937..28476)
                     /gene="rsmD"
                     /locus_tag="ACL8CL_000434"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HGS1329738.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEGGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            complement(28541..29533)
                     /gene="asnA"
                     /locus_tag="ACL8CL_000435"
     CDS             complement(28541..29533)
                     /gene="asnA"
                     /locus_tag="ACL8CL_000435"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HGS1329739.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            complement(29644..30327)
                     /locus_tag="ACL8CL_000436"
     CDS             complement(29644..30327)
                     /locus_tag="ACL8CL_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HGS1329740.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYFLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            complement(30383..31123)
                     /locus_tag="ACL8CL_000437"
     CDS             complement(30383..31123)
                     /locus_tag="ACL8CL_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HGS1329741.1"
                     /translation="MTIITSKANSVVKNTKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQREEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            31157..31435
                     /locus_tag="ACL8CL_000438"
     CDS             31157..31435
                     /locus_tag="ACL8CL_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HGS1329742.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYSDFKIAN"
     gene            31513..32439
                     /gene="yidC"
                     /locus_tag="ACL8CL_000439"
     CDS             31513..32439
                     /gene="yidC"
                     /locus_tag="ACL8CL_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGS1329743.1"
                     /translation="MKSIKRFAFSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPPKASAFSK
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(32514..33308)
                     /gene="pflA"
                     /locus_tag="ACL8CL_000440"
     CDS             complement(32514..33308)
                     /gene="pflA"
                     /locus_tag="ACL8CL_000440"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate-lyase-activating protein"
                     /protein_id="HGS1329744.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTRQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKKLMDTESYQDYMKRVHG"
     gene            complement(33434..34684)
                     /locus_tag="ACL8CL_000441"
     CDS             complement(33434..34684)
                     /locus_tag="ACL8CL_000441"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HGS1329745.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEAFAILKEKGAKTFGIHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     IWLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            complement(34751..35578)
                     /gene="purR"
                     /locus_tag="ACL8CL_000442"
     CDS             complement(34751..35578)
                     /gene="purR"
                     /locus_tag="ACL8CL_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0045982 - negative regulation of purine
                     nucleobase metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HGS1329746.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            complement(35845..36786)
                     /locus_tag="ACL8CL_000443"
     CDS             complement(35845..36786)
                     /locus_tag="ACL8CL_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HGS1329747.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVAHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMTNKIFAMDNRSFYKPDLD"
     gene            complement(36776..38032)
                     /gene="rmuC"
                     /locus_tag="ACL8CL_000444"
     CDS             complement(36776..38032)
                     /gene="rmuC"
                     /locus_tag="ACL8CL_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HGS1329748.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVVVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEAAYETGDKDEIERCRKSLLASVKRFARDIRNKYI
                     APPRTTNFGVLFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            complement(38034..38696)
                     /locus_tag="ACL8CL_000445"
     CDS             complement(38034..38696)
                     /locus_tag="ACL8CL_000445"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HGS1329749.1"
                     /translation="MSEYKLSENNWTRVAVFAGGNRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKGAQYFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGISQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            complement(38659..39315)
                     /gene="rpe"
                     /locus_tag="ACL8CL_000446"
     CDS             complement(38659..39315)
                     /gene="rpe"
                     /locus_tag="ACL8CL_000446"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HGS1329750.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGEVNERVQTLRKQLD
                     "
     gene            complement(39326..40204)
                     /gene="rsgA"
                     /locus_tag="ACL8CL_000447"
     CDS             complement(39326..40204)
                     /gene="rsgA"
                     /locus_tag="ACL8CL_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HGS1329751.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYQQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKIAPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            complement(40206..41078)
                     /gene="rsmA"
                     /locus_tag="ACL8CL_000448"
     CDS             complement(40206..41078)
                     /gene="rsmA"
                     /locus_tag="ACL8CL_000448"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HGS1329752.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPDLPIKVVANLPYYITTPILMHLIESGIPFCEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFTHRRKTLWNNLTGYFGKTEEVKDKLTKALDQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            complement(41209..41517)
                     /locus_tag="ACL8CL_000449"
     CDS             complement(41209..41517)
                     /locus_tag="ACL8CL_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329753.1"
                     /translation="MCEKIRIRRVSDYPSARGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEGQRQKHYRLKNLPQMVELTVDDVEEDVDLTLPENRSYQEADFFERMFRENC
                     "
     gene            complement(41510..42151)
                     /locus_tag="ACL8CL_000450"
     CDS             complement(41510..42151)
                     /locus_tag="ACL8CL_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329754.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            complement(42153..44183)
                     /locus_tag="ACL8CL_000451"
     CDS             complement(42153..44183)
                     /locus_tag="ACL8CL_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGS1329755.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREEVTEALSHLAVEHNSLIARRIVEPNEAGETRFTYATYGEGKLPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSL
                     LGLGILWYQGALFMATVQLVIIALLLYGLTLAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRIMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQSSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSVYYQGLFTDYL
                     QNFSSESVEVTSQKHYLPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEKGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            complement(44260..44556)
                     /locus_tag="ACL8CL_000452"
     CDS             complement(44260..44556)
                     /locus_tag="ACL8CL_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGS1329756.1"
                     /translation="MKQTVKKLALVASIAATLGGGVSVASAAVQYPEGGVWTYGSGNG
                     GAYSNYYHPSKYHSSTVVSRKTGSSDKGYAGAGGTSRAWIRTSWGEKVAFYYNV"
     gene            complement(44876..45775)
                     /locus_tag="ACL8CL_000453"
     CDS             complement(44876..45775)
                     /locus_tag="ACL8CL_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1329757.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     LPKLAYIARRLGVPVYSLMPDFSALPSAYLELKYQILREPIYGKEEEYDKKEACLEEI
                     YKTYFDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEAYSINDMLL
                     IRLFFYQMLIRKDLAKFINQIEKLMLFLLEQKKVTQIENYFIIRDTLISGMCCLEKVG
                     VTDCFNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAK
                     LIGDGFLVKKLTEEWQEDVKKYL"
     gene            complement(45847..46407)
                     /gene="rnmV"
                     /locus_tag="ACL8CL_000454"
     CDS             complement(45847..46407)
                     /gene="rnmV"
                     /locus_tag="ACL8CL_000454"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043822 - ribonuclease M5 activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HGS1329758.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLAGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            complement(46407..47180)
                     /locus_tag="ACL8CL_000455"
     CDS             complement(46407..47180)
                     /locus_tag="ACL8CL_000455"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HGS1329759.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALAAEMGVIQMNIVGFDKPTIE
                     RALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHPKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKILVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAVATTANAERIFGLDSK"
     gene            complement(47485..48378)
                     /locus_tag="ACL8CL_000456"
     CDS             complement(47485..48378)
                     /locus_tag="ACL8CL_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall surface anchor protein"
                     /protein_id="HGS1329760.1"
                     /translation="MVAQDPKAQDSTKPTAEKSTVKAPAQRVDVKDITHLTDEEKVKV
                     AILQANGSALDGATINVAGDGTATITFPDGSVVTILGKDTVQQSAKGESVTQEATPEY
                     KLENTPGGDKGGNTGSSDANANAGGGSQAGGSAHTGSQNSAQSQASKQLATEKESAKN
                     AIEKAAKDKQDEIKGAPLSDKEKAELLARVEAEKQAALKEIENAKTMEDVKEAETIGV
                     QAIAMVTVPKRLVAPNAAPKTTSAPQATAGTMQDVTYQSPAGKQLPNTGSASSAALAS
                     LGLVVATSGFALLGRKTRRRK"
     gene            complement(48662..48796)
                     /gene="rpmH"
                     /locus_tag="ACL8CL_000457"
     CDS             complement(48662..48796)
                     /gene="rpmH"
                     /locus_tag="ACL8CL_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HGS1329761.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            complement(48941..50155)
                     /locus_tag="ACL8CL_000458"
     CDS             complement(48941..50155)
                     /locus_tag="ACL8CL_000458"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS1329762.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            complement(50311..50604)
                     /locus_tag="ACL8CL_000459"
     CDS             complement(50311..50604)
                     /locus_tag="ACL8CL_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329763.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNLLYDSFLGLLFLGLSFGM"
     gene            50766..51218
                     /locus_tag="ACL8CL_000460"
     CDS             50766..51218
                     /locus_tag="ACL8CL_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HGS1329764.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIAHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            complement(51253..52641)
                     /locus_tag="ACL8CL_000461"
     CDS             complement(51253..52641)
                     /locus_tag="ACL8CL_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1329765.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKIVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDMLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            52707..53669
                     /locus_tag="ACL8CL_000462"
     CDS             52707..53669
                     /locus_tag="ACL8CL_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HGS1329766.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVNTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            complement(53698..53901)
                     /locus_tag="ACL8CL_000463"
     CDS             complement(53698..53901)
                     /locus_tag="ACL8CL_000463"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329767.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDRTDEVSNHTYGKAT"
     gene            complement(53965..54975)
                     /gene="ccpA"
                     /locus_tag="ACL8CL_000464"
     CDS             complement(53965..54975)
                     /gene="ccpA"
                     /locus_tag="ACL8CL_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001228 - DNA-binding transcription
                     activator activity, RNA polymerase II-specific [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HGS1329768.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGIAYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(55400..55999)
                     /locus_tag="ACL8CL_000465"
     CDS             complement(55400..55999)
                     /locus_tag="ACL8CL_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1329769.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDVESVLQAKNGQEAIQLLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRSERLETKVVVVTTFKRAGYFERAVKAGVDAYVL
                     KERSVADLMQTLHTVLEGRKEYSPELMEVVMTRPNPLTEQEIAVLKGIARGLSNQEIA
                     DQLYLSNGTIRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(56001..57098)
                     /locus_tag="ACL8CL_000466"
     CDS             complement(56001..57098)
                     /locus_tag="ACL8CL_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1329770.1"
                     /translation="MLERLKSIHYMFWASLIFMIFPILPVVTGELPAWHLLVDILFVV
                     TYLGVLITKNQRISWLFWGIMLVYVAGNTAFVAGNYIWFFFFLSNLLIYHFGVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYMLVILTFVDLMTLGSVRIRLVEDLKEAQ
                     VKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQMQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVETDNQLDTASLTQEL
                     ESMASMILLELVTNIIKHAKASKAYLKLERTEKELILTVSDDGCGFAFLKGDELHTIR
                     DRVFPFSGEVSVISQKHPTEVQVRLPYKERN"
     gene            complement(57098..57835)
                     /locus_tag="ACL8CL_000467"
     CDS             complement(57098..57835)
                     /locus_tag="ACL8CL_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1329771.1"
                     /translation="MKRWIALSKIEFLLTKRQLIYYLLSVGMPTAFYLFFSGIYQDTP
                     GELANFMRDYLISMTAFSMMSTTILSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMLVDYLVSILVVFTVGHFVRGVDMPLVKWVGAAILLIVGSIAFVALGLTLTLLPTS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAVGKCLPTYQLMELLKTFLNEGGINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(57837..58721)
                     /locus_tag="ACL8CL_000468"
     CDS             complement(57837..58721)
                     /locus_tag="ACL8CL_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329772.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVSIQGLPVTSSKLDYTRAYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLSNQEIDQLLQFVKQQKEQLAEKLSGGQKRLFSFVLTLIGRPKIVFLDEPTASMD
                     TSTRQRFWEIVQELKAQGVTILYSSHYIEEVEHTADRILLLNKGELIRDTTPLAMRSE
                     EIEKHFILPIAYKEVVEQSNLVENWTLKQDSLQVVTREADAFWELLAQAGCRMQEIEV
                     NNRSLLNTIFEETQKGDN"
     gene            complement(58708..58902)
                     /locus_tag="ACL8CL_000469"
     CDS             complement(58708..58902)
                     /locus_tag="ACL8CL_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329773.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            complement(58899..59105)
                     /locus_tag="ACL8CL_000470"
     CDS             complement(58899..59105)
                     /locus_tag="ACL8CL_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329774.1"
                     /translation="MGEEEMRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT        17407 a        13502 c        11140 g        17638 t
ORIGIN      
        1 agggtatgga tccacatggt ctgagtggtt gtttggttgg ttattcgtgc aatactcgtg
       61 cgttttaatt ccagctaaac tccctgtatc aagcgttttc ggcaaacctg cttcatctgc
      121 tagattgcgt aagagttcga tataaaggcg gtagtccgtc atgaactctt ctttagttga
      181 atggctttca atcagttcaa ccgctgcata ggtctcagca ttccaaccgc ccccaacgtc
      241 ccaggcacca ttatcaacag gtcctacctg catgatgcaa ccgttcccaa caatgtgcga
      301 gaaaaaacct aattctgggt ctttccgcca gtgataatcc gcttcattct gtacggttga
      361 atgcggattc ccagttgagt gtgcgtgtac ttgcctatat ggttgcacgc cgacttgagg
      421 caaatctgtt cttaatttac tcacattaat ttccatattc tactccttat caattaaaac
      481 aactcatttt ttacaatcca gaaccagaaa ctcctttatt tctaccttac aaagaagaca
      541 attttagtca cgattaggct tgtagataga acttcaaaac gcactatttt gacactgtaa
      601 ataggactga caaggtctgc attctatcta caataacacc ccaaactaaa aagcttttca
      661 aaagtatatt tttacagtct ctatatgtcc ttttcataaa tactatactt tattatatca
      721 tataaaagaa gtcaaaagtc tgttaaacta ttttcaacac caaactaaag aagagaacac
      781 aagttttttc gattttcact agaggaaatg gattttattc agtaaatcca actaggattg
      841 cactttggtt gccaaaattg cctttccttc ttttatcaag ggatgacgga atagtgagaa
      901 gtacagttga actgtcatgg caacccagat aagaggttca caaaggataa cacccttata
      961 tcctgcccaa ggaataatca aaaccacaaa aacgattttt ccgattagtt caataaagct
     1021 agaaactaga ggaaggatct tttgccccaa gccctgcaag caattgcgat aaatcaacaa
     1081 gaggctcaaa atgggataaa aggctgaact gatttgcaga tagagacttc cattttctat
     1141 caagtaacca tctgtcgaac tagccaagaa ggaaaccaaa gctggactgg ccaaaaagag
     1201 gaaaatacaa acaaaaactg cccaggatat acttaaacga ctgccgattc gaagaccttg
     1261 aacaatacgg tcaggtcgct tagctcctag attctgagaa gcaaaggtcg tcattgatgc
     1321 agaaatagcg gtcataggaa gaagggcaaa ggtcataatg cgtcgagctg ccgtctgggc
     1381 actaataatc actgcaccaa atgtattaac agaaaactgt aaaatcacac tgccgataga
     1441 tacaattgaa ctcatcaagc ccatagccaa accttgctcc aagagatccg cgtacaagct
     1501 tttgtcccat ttgaaatgtt taaactgtgg caagagttct ggcacacttt tacgaatata
     1561 ataaaagcag agaaccgctg ataaaccttg cgaaataatg gtagcaagtc ctgcggattg
     1621 aactcccaga tgcaattgcg taataaaata gagatccaga accacattaa ccaaggcaga
     1681 gaaaatcaga aatcccaggg ctgctagact gtcaccaata gaccgcaaca agcctgcaaa
     1741 aagattataa gcaaagctga cacctacaca ggtcacaatc atagaaatat attgataaga
     1801 ttgaggaaga atttctgcag gagtatctaa gtattgcaag agaggataca agccaagaaa
     1861 gcccagcaac ataactagaa tgctcaaaag agcacctaaa atccaagtgg ctgctactgc
     1921 ttccttgatt ttagtgaaat tccgagctcc ataataacga gcaatgacaa tccccatgcc
     1981 attgccaaca ccaagtgtaa aacctacaat caggtcaaaa atcgctgtcg tcgctcctac
     2041 tgcagccaag gattcttgac caagaaatcg tccaacaatc aagacatcag cagtgttata
     2101 gagctgttga aaaatatttg atagcaagat tggaaaggtg aagcttaaga gcgagggaag
     2161 aatcggacca tgtatcaggt ccactgttcg ttttttattc ataaagctat tatatcagct
     2221 tcctagagga gcgacaagaa acagcaaact tatatatagc ggaatgaaat aaggacagtc
     2281 aaatcgattt ttaacaatgt tttagaagta gatgtgtact attctagttt cgatctatta
     2341 tagttggttg agtttggaat agtacgctgt agctgctaaa acatttctag aaattaattt
     2401 gactttccta ataaagttgt tcatatctta tttcaagcta ctatatataa tctaaaaact
     2461 tgaatcaaaa atcgaaaaag tagcggattt ctccactgct ttaacagctt attcttcaat
     2521 ttcgatttca agttcatcgc ctaagtcaag cgtaacttct tcattcactg cttcttcttt
     2581 ctttggctca tcttttttgt tttcagtatc ttgttctgaa acttcttctc catcaatcaa
     2641 gccaaattta gaacggactt gcttatcaat ttcatcaaag atttctgggt gctctgccaa
     2701 gtatttctta gcattctcag aaccttgccc aattttttca tctttgtaag aataccaagc
     2761 ccctgctttt ttgataatat ccaaatcgct tgcaatcttc aaaagctcac cagtcttaga
     2821 aattccttct ccgtacataa tttcaactac ggcttcctta aacggtggag ctaccttatt
     2881 ttttacaacc ttaatcttag tttctttacc gacattggtt tctttttggt caccagttcc
     2941 cttaatttgt gtattaccac gaacatccaa gcggactgaa gcatagaatt tcaaagcacg
     3001 tccgcccggt gttgtttctg gatttccaaa catcactcca actttttcac gcaattggtt
     3061 gataaaaatg gcaattgttt tggttttatt gatagaggcg ccaagtttac gcatggcctg
     3121 gctcatcata cgagcctgca aaccaacatg gctatctccg atatctccat caatttccgc
     3181 acgaggaaca agggcagcaa ctgagtcgac tacgacaaga tcaactgcac ctgagtcaat
     3241 caattttccc gcaatctcaa gaccttgctc tcctgagtct ggttgagaca agagcaattc
     3301 gtcaatattg acaccaaggg ccgcagcata agctggatca agggcatgtt ccgcatcgat
     3361 aaaggcagca atcccacctt ctttttgcgc ttgtgcaact gcatgaaggg caaccgttgt
     3421 cttaccagat gactctgggc catagatttc gatgatacgt cccttaggat aaccacctga
     3481 gccaagggca atgtcaagag ctaaagaacc tgagctcatc acttgcacct tttgctccgc
     3541 acgttcaccc aaacgcatga ttgatccttt accaaagtct ttctcaatca atttaagagc
     3601 gtcattcaag gccttttcac gttctgcccc aaattttttt gaaatttctt ctaatttttt
     3661 tggttttttc gccattctat tctcctacat tctaatgatc ctcagaccta tctactatta
     3721 tatcaaaagt tagtcactta ataaagcctt gcgaactagg ttaaaggcat gcataaccgc
     3781 aatgtgacgt acatctgctc ggcttctgcc tccaatatta accttgataa cctcagttcc
     3841 ttgatcttgc gccaagccta tgaagactgt cccaactggg tgtccttcta ggctatctgg
     3901 tcctgccact ccagtcaaac taattccaaa atcagactgg gtcttgcttc gtgcctgctc
     3961 agccatcttc tgagctgtaa attcagacac cacaccatgt tcttccaaat tcttggcagg
     4021 aatatccaac atccttgatt tttcctccaa gctataggtc acaaaaccac ccttaaatat
     4081 acttgaaact ccagaaaaat tcgccacggt agcttggaaa agacctgccg tcaaactctc
     4141 tgcagccgcg atggttttcc cttgcctttt cagttcttct accacaatgc tggctaaact
     4201 agtttcttcc ccataaccat agcaaaagtc tcgtaaagaa attccttcga aagtctggca
     4261 gtccaagatt tgattttcca agatatccag cgcttgattc gcctcttctt gactgctagc
     4321 ctttgttgac agacgtagag tgacttctcc tgtcttggca taaggggcca aggtaggatc
     4381 gatctgatta tcaattaaat cagccaaaat cgtaaccaac tggctctcgc caatcccaaa
     4441 gaaacgaaga actcgggaat acagcttgct ccctgtcatc aacttgggta gaagttggtt
     4501 taagaccatg ggtttcaatt cacttggcgg acctggaagg acgacatagg tcactccgtc
     4561 tacttctaat tttcctccaa cagccagtcc tgtttcgttt ggcagtggaa tcgctccttc
     4621 tacaatttga gcttgtcttt cgttattcgg tgttcgggca tagtctggtc gcagggcaaa
     4681 aaagatatcc aacttctcct gagcctgagg atcaaagact aatgctttcc ctaaaaattt
     4741 agctagggtt tgtttggtta ggtcgtcctc agttggcccc aaaccgccag tcaaaatcac
     4801 cagactgcta cgttgactgg caatctcaag caaagacaag agacgaactt cattgtctcc
     4861 tacagccgtc tgaaaatata catctacccc aatctcagct agtttttccg acaaaaactg
     4921 ggcattggtg ttgacaatct gtcctgtcaa aatctctgtt ccaacagcaa tgatttctgc
     4981 tttcatgttt cctcctacct atctattcgt atttttttga aaaaatcgca ggaattttcc
     5041 tacgattgat ttttttattt gtatcaaaag ttaattatct tcatcaccaa caggtgctct
     5101 gccaaataaa tcttcaaata aaaccgcatt ggtttcaagc tgagtaactt cttcttgtcc
     5161 caaagaacgt cggagtatat tttgcatttc caacatatgt gctctcgaaa caatctggta
     5221 agaaacacct tgaagtatct ctccttcacc ctgcaactgc tgagtttcaa tggttttaaa
     5281 tgaatcttta tagcctagca agttagggat actttttgca gacaaatcaa tattggtctg
     5341 catattgtca ctcaaagctt ttagaatctc ttgataatga ccaacgctat ttaaactgag
     5401 agctttttcc atgacttttt gaataacttc acgttgacgt ttttgacgac cataatcccc
     5461 ctcaggatct tggtaacgca ttcgtgcata gactagggct tcttctcccc caatatgttg
     5521 ctccccaaca ccgatagaaa tagtattaaa ttcttcttgg tcactgatag aaattgggaa
     5581 acctaggata ttattgactg taatacctcc tactgcatcc actagttttt gcaaccctct
     5641 catattgacc atcacatagc gatcaatatg gatattcatc attttttgaa tggtttctat
     5701 agcaagctct gctccaccat ctgcatatgc tgagttcagt ttcgcttcat gagcctgacc
     5761 attccctgat tcaatgcgcg tcagaatatc ccgctctaaa ctcatcattg ttgttttttt
     5821 cgttttagga ttcactgtca tcaagatcat gctatcactt ctaccgaccc aagtttcagt
     5881 tcgttcagca tttccggtgt ccactcccat taacagaatg gttagaggtt cagtcgcttc
     5941 aataaccttg gtttcttcgc cgattttttt ataggtttta gctaaggttt ctgtcccttg
     6001 ttgataaata gtataagcaa aaacacctac tcctactaca gttacagaaa gtaaagctag
     6061 caccattcca ataatttttt taaccatatt tctactaacc tatcagttta cccatcaagt
     6121 aaacatcgat aaatttccct tcttctatat atgccccacg ctcttggcta ccttcaatga
     6181 caaagccatg cttttgataa agatggactg ctgcttgatt acgagtttgg acagtcagtt
     6241 ggagacgacg cagaatgcca cttgcttgtg cccactctat cgcttcttct agcaacaaac
     6301 ttcccaagcc attattccaa tatctttttc caatcacaat gaagagatct ccaatatgac
     6361 ggactctctt acgctgatca gctgtaatat ttacaatacc agcaattttg ccatttaaga
     6421 atgcaagtaa ggttatctga ttgtccgaac tagcttgctt gttgaggaat atttccatct
     6481 cctcactagt caagagaata ccatctccgt ctaggctggt aaagtctgtc tccaaactca
     6541 cacgatttaa aaaggccact aattcagctg catctttggg ctctgcttcc ctaatgagca
     6601 attcatactc catattgaag ctcctctaac aatttctcag cacgcaaacc ctttgcctga
     6661 aaatttaaac ggcgtccatc tgcttctttt agaatttcca attctaaata agcatctggc
     6721 aaggcatctc ctaagagatt tccccactca ataacagtca cgccgccacc aaagataaac
     6781 tcatccaagt cgatagaatc agcatctcct tcaatacgat aaacatctag gtgataaagt
     6841 ggaagtcgac cctcatactc tctcacgata gtataggtgg gacttttaat catttgagaa
     6901 atctgtaatc cttttgcaag tcctttagta aaggtcgttt tacctgcacc cagttctcca
     6961 gttaagatta aaacatcatt ctttgctaat agatggccca aacgctcccc taaggcttgc
     7021 aactcttctt cattttttgt gtacatactc ttattatacc aaaaactttt cttttgtgtc
     7081 tattttccta ctaaacttat catcataaca tccataaaaa acaggctttc tctaaaagaa
     7141 aatgagcgta acaatgacca atacaagatc tcggaaaata tgaccataaa aggaaacttc
     7201 cttcttaacc gaatttggga caagataggc tgcaaaaaac aagcccagtc caatataaat
     7261 cagaagtgag acaatggtca ttggatttct taagaaaaga agtgttgcta aaatagtcac
     7321 caacactgtc ttttttctgt ccagcatagc aagaaaatcg cgcacgtatt ttttcaaggg
     7381 taaaaaaatc agcaaatcta gcccaaatag gaaaaagaag gatggcaata aaaagtcaac
     7441 taattcttgc tgcagcgtat ttttgatgaa caagttatct gacaaaacaa gaacagctcc
     7501 taacaaatta attaagagta acatactgta aaaaagcttc accgacttct tactggctag
     7561 gacactatgg acttcttgct tacgagtata aagataattt actccagcac agattcctga
     7621 aacgaaaacc atgcttccga tgaaaaaagc tgtactttgt ttaaaggaca agatgcattc
     7681 cttccatagg aaacagctac tcaaactgat ttgaattaaa gctaacaaaa ataagattct
     7741 cattgatttc atcttctctc tcccttccta ccaatcatta tactaggaga aaagagagaa
     7801 ctgtttctaa tcttctcaaa tgtctcttta agacgctaaa caaacactag agactaatac
     7861 tcaatgaaaa tcaaagagca aactaggtag ctagccacag gttgctcaaa acactgtttt
     7921 gagattgcag atagagctga cgtggtttga agagatttac gaagaatata aatttgaaat
     7981 catgaaaatc cgtcaaacgg gtggttgttt tgtctcgcac ctcacggagc gagacggact
     8041 cagagtcaca taattataag gctgatagta ttaatctaac tatcagcctt acaggttatt
     8101 taacgtttca gaaaaactat aatgtcaaga ttaactaaac agtatctagt tccttcaaat
     8161 aattttctat cttcatcaac attaaaggat tgttataaat cttacataac tctcttgctt
     8221 ctatataata atttttgact tgttctctgt ctagaaattt ggctccagca tttcctacaa
     8281 gaataagtag aggagccaat tggtagcttg tctgtctttg tttacagagt tcaatcgttt
     8341 caagagcttc ttggatggct tcattatatt tttcctttga tactaggtag tgagcgtagt
     8401 tgtaacgaac tctgatgtag ccaaataaaa actcttgatg gtccaaattt tttgtctgat
     8461 acaactctat taaatgagag tagtttgcct catattcttg ttcacgaccc actaaggaat
     8521 agaaattaga tagagtattc aacgccttta aataaatcag agtatttgaa gagactttta
     8581 ataatatatt ttccaatgaa gaaattgcct cacacttact gtcatattga tagaagtcaa
     8641 taatagcttt aatccattca aggtaagttc ggtcttctag tgttagaaaa gtacttcgtt
     8701 caatctctat tctataaata tattccaaat cgtcataatt tctatcgtct aatagtcgag
     8761 cagacaattg cttgaaatta gagaggttag atttaatttc aatttgttca ttaaaaaaat
     8821 aatctaatgg tacttcaagt cgttgagaga gtttgaacaa aaggtctgcg gagggaatga
     8881 aatgccctct ctcaatttta ctaatctgac tttgttcaca aattccttct gcaagagttt
     8941 gttgggagag tctcaactct tttcttttca atctgaattt ctctgcgagt gtattcatta
     9001 aagataataa accttcctat ttgcttaatt gcattataaa atttttagtt caaaatagca
     9061 agttaaagga ataaataatt ctttatggta ataaactaaa aaaagtcaaa aattacataa
     9121 aaatgcttgg ctaaataaaa tatatctgtt aaaataagga taatgaaaaa gaaaagggtt
     9181 tcacgaatga agaaaaatcg tggaattcaa aaattagcta tattagtatt actaggtgtt
     9241 tttatgttta gtaatacaat tccttaccaa cagtttattc agaagaatag acaattggag
     9301 attcgagtgc aatcgcaaaa gaagtccaat ggtcttgatg ttgggaaggc tgattgattt
     9361 cgtattactt taaaattcaa ctatttagag aaaggtattc tgatgagagc ttgtaacaat
     9421 accagataga tataagaatg gagatgtttg ataaaatgct ttcatagcta accaactgtt
     9481 gaaaattatg agttacaaaa tctaaagtat ttaagattgg tttataaaat taggtctctg
     9541 cctgtagaaa ggaacgtgca gttagaaagg aggaaaaaat aggatactat aattcagact
     9601 gagatttcta attaaaatat atagttgcat ttcgctcatt ttcataatat ttcattcaga
     9661 ggagtaataa tatgttaaaa tttagaaaag tagttgcatc atcccttgcc gttttggcat
     9721 ttagtttggc tcttcctgtt ttggcagttt ctcaccgtgg tggcgagtgg acctatggag
     9781 gccatcatga tccaaataat tggggagcat tttcgaatta ttatcatggt tcacagtatc
     9841 actgggccta tgttggaagt aatggacgta acaatcaaaa aacagtttat gcaggagcac
     9901 gtagtgcggc ctatgcgttt gttaatacaa attttgggga gcaagttaca tttgatgcgg
     9961 gatggtaaag aataattgaa gaagattctt ttatcaatag ttcagtaagc ttgttagttt
    10021 atagagggca ggagtatttc tgccctctgt tttagaataa ggaatgcact atgaaaaaat
    10081 tatttatact catatccaac ctgcttgcta gtttgttctt tgtttgggta tttaccattt
    10141 ggactgatac atatgtctcc cattattacc ccaacgttgt tgtacgtgat tcttcccctg
    10201 aaacaacttt tcagcatgtt gctacacgct tggaaaaact agcagaagaa acagatagct
    10261 ttattgcgat tcaacaccaa gatcctaact cagaaggtac tacagttttt tcttatacga
    10321 cctttgggga cggaaagttg cctgatgggc ttcaggaaaa aaatctagaa gatgctcaaa
    10381 gtagtagtgt tgaaacaaac tattttgtat tcgatggaca cctagatatt cacttgctaa
    10441 gggaagagct aagtcaactt ggcttgacta acatgcacct gacaattcca tctaaactat
    10501 ctacgttaat ggctatcttt agtaacggat ttcagttaat cagtctattg attttcatct
    10561 taacctttgt agcactgact ttaattagtc aaattagcca attacggtca tcgggcattc
    10621 gcctcatttc aggagagaaa cggtggtcca ttttccttag accagttggt gaggatttga
    10681 aagggatagc tgttggtttc agtttagctg gcgtgctcgc cattcttatg caaaaaattc
    10741 tgtcgcttcc aacgcaatcc ctaatgacca taggagcagg cttgctcagt tataatctca
    10801 tcttgttgtc gatctccctg tttttcgctc aactcttcgc agttgggata aaaaagatac
    10861 acctgatgca gattataaaa gggcaagtgc ctgtcagagg aatcattagt ttgattttaa
    10921 tcggtcaact gttagcgatt attattgtaa cgctgggaat agggagtagc ttaaagtatt
    10981 cccaagcctg gcaacagcat cggattggac aagaggtttg gagtcaggaa agacaactga
    11041 ttaccctatc aatcagccgt gaaggaacga gtcctggttt tgatgaacaa gctcaaagga
    11101 aactcagaac ttggtatcaa ttgatggatc tggctgtttc agaacaaaag gctttcttat
    11161 ctagacacca gttaattgac cgtactttgc aaaatggcat ggcttcctcc aaaaacttga
    11221 caacctctac agaatggcac gattacagcc cgaatggcaa tgtccttatc gtcacaccgc
    11281 attacttgga gcgtcaaaac attcctgtag atacaactat taagcaaaag atgaatcacc
    11341 ttaatgtagg ggagtttgtc ttattgctgc ctgaacacct ccgttcagag gaagaacatt
    11401 ataaatctgt ttttgaagac gacttaacca gtcgcatgtc tagtcaagat gaacgacagc
    11461 aaatgactgc tacggtaggt tatttagaat caggtcagga tcgttttgtg tataatacga
    11521 cccctatttc ttaccagcag tttttgaaag atccaatcat cattgttata acaccccaat
    11581 caactggtcc acagtccatt ttgttttgga tagacgcagt acagaactac gttctcttta
    11641 atcaattgtc tgatgcccag gagcttatcc agagacaagg cattgaaaat tgggtctcag
    11701 aaatgcaaac aggttaccac aactacatca cattattgga taatatccag agggaacgtt
    11761 gggtaatgct agcaggagct gtgcttggga ttgcaacttc aatcttgttg tttaacacta
    11821 tgaataggct ctactttgaa gaatttagac gtgccatttt tatcaaacgc attgcaggtc
    11881 tcaggttctt agaaatccat cgcacttatc tctttgctca actgggtgtg tttttactgg
    11941 gatttgttgc gagtgtattt cttcaggtag agataggagt tgctttctta gtcttgttac
    12001 tctttactgg tctatctctt ttacagttac atgtccaaat gcagaaagaa aacaagatgt
    12061 ccattcttgt tttgaaggga ggttaatatg attgaactta aacaggtgag taaatctttt
    12121 ggagaacgag agttattttc gaatctttca atgacatttg aggctggaaa agtctatgcc
    12181 ttaattggtt caagtggtag cggaaaaaca accttgatga acatgattgg gaaattagaa
    12241 ccttatgatg ggacgatttt ttaccgaggt aaagacttgg ccaattataa atcgagtgat
    12301 tttttccgtc acgaattggg ctacctcttc cagaactttg gcttaattga aaaccaaagt
    12361 attgaagaaa accttaagct aggtctcatt ggtcaaaagt tgagtcggtc ggaacagcgg
    12421 ttgaggcaga agcaggcttt agaacaggtc ggcctggctt atcttgacct agataagcgc
    12481 atctttgagt tatcgggcgg agaatcgcaa cgagttgcct tggcaaaaat tatcttaaag
    12541 aatccaccct ttattctggc agatgagcca acagcttcaa tagacccagc aacctctcag
    12601 ttgattatgg agattttgct atctcttcga gatgataata ggctaatcat tatcgcaaca
    12661 cataatccgg caatttggga gatggctgat gaagtgttca cgatggatca tctgaaataa
    12721 aaatccttgt ttttaattgc acgatgagtt actgaaatat tatcatgaat caataattgg
    12781 agttaattta gaattgtact ttattaatat tgaggtaact ttttcttgat aaaggaggaa
    12841 atagtggaga ggaagttaga atgaaaaaat tcgacaatta tattattgag aagccttgcg
    12901 attctaattc agataaactg caaaaaatct taataattga aaatttggta gatgatattt
    12961 tgcaatttcc tctcagaatc aataatagtg taggagagat tttcctccta caaccgtttc
    13021 aaaagaaaac tatctttatt ccatgttatt ttgaggaaga tattgtgaaa gtcaaagatg
    13081 atgataaagt tgagtggaat ttgttagaat ttcaaaaatt tagagcattt ttggcttagt
    13141 aatctgtgtt gaaggctcaa aacctatggt aaaaaagtcg ctttgaaaac gtattgcctc
    13201 caaagattta gttaaataat gatttaacac aaaaagaaat tattgaagtt ctggaaagat
    13261 gttgtttcag tattgagaaa aggtgggaaa aacttgcgat tttcacagag aaaggaagaa
    13321 aaagtataga aatatagtca attgaaacaa gaacaggaca aaagaacctt ttgtgccata
    13381 tttttctcct ttcgctttac aattggcttg aacaccttta ttgtatcgcg tttggagttt
    13441 ttttggtata accttcgacg cgcacccgca tagcgggtgt ttttttgtct cgcacctaac
    13501 ggagcgagac gaactaatag tcacttaatc aaaaaacgca ccatatcaaa aactaaaaag
    13561 tttgatatca tgcgtcatgt cttaaactaa ttgactatac tttctattca aatgagcttt
    13621 taaccaattg attgagccaa tccactctta aaaccaaaga gcaatttctc gcttagctga
    13681 ctcttctgaa tctgaaccat gtacaacatt ttggataacc tcattttctc cagcagcttt
    13741 tgcaaagtca cctcgaatag tgcctggtaa agattcttct ggacgagttg cacccatcat
    13801 ggtccgccaa gtttcgatta ctttgggacc agaaatgaca cccacaagaa ctggacctga
    13861 agtcatgaat tcacgaatcg gtaggtaaaa actctgacca accaagtcct gatagtgctg
    13921 gtcaatcaac tcttctgaaa cctgtgaacg aaactccaat ttttcgattg taaatccacg
    13981 ttgttcgatg cgctttaaca cttcacccac tagccctctt tttacaccat ctggtttgat
    14041 gataaagaat gtttgttcca tacccgtctc ctttgtcagc ttctttcttt tattttacca
    14101 catttcgtgg aaaaatggag aaagttttca gaagagagaa tgagagaacc cgagggttct
    14161 ctcattctct cttattctac tgtttcttcc acagtttcaa cggcagtatc cacaactact
    14221 tctgttgttt cttcatttcc ttcttcctct actggaggat taaggtattc ttcttcgttg
    14281 acagcatgtg gttcaaggtt acggtaacgg gccataccag taccagctgg gatgatctta
    14341 ccgatgataa cattttcttt aagtccaagg agatggtctt tcttaccacg gatagctgcg
    14401 tcagtaagga cacgagttgt ttcttggaag gaagctgctg acaagaaact gtttgtttca
    14461 agtgaggctt tggtaattcc cataaggact gggcgacctg tcgctggaac tccacctgcg
    14521 ataaggacat ctttgttggc atctgtaaag tcattgatat ccatgagggt acccatgaga
    14581 agatctgtat cacctggatc catgacacgg actttacgga tcatttgacg aaccattacc
    14641 tcgatgtgtt tgtcaccgat ttctacccct tggctacggt aaactttttg tacttcaccg
    14701 agaaggtacg tttcaactga caagacatca cgaactgcaa ggagacgttt tggttggata
    14761 gaaccttctg tcagagcagc accacgcgct acttggcccc caacttcgac acgcatacga
    14821 gctgtaaatg gaacgacata ttcaccttcg ccagtttcac ccttaacaaa gactttcttg
    14881 gtacgagttg atgcatcttc ttcgatagca gtaacttgtc ctttaacctc tgtgataacc
    14941 gcttcccctt taggattacg ggcttcaaag atttcttgga cacgaggaag accctgagtg
    15001 atatcggtat ttgaggcaac cccacctgtg tggaaggtac gcattgtaag ctgtgtacca
    15061 ggttccccga tagattgggc agcaattgta ccaactgctt caccaacttc aaccgcatca
    15121 ccagtcgcca agttgatacc gtaacagtga cggcagacac cgtgacgagt gttacatgta
    15181 aatacagaac ggatagtcac ttcttccaca ccagcattga caatttcacg cgccttgtct
    15241 tctgtaatca attcatttgg accaataatc actgcaccag tttctggatg tttaacagtt
    15301 ttcttagtgt aacgaccgtt gagacgctct tcgagagact cgatcatctc ttttccttct
    15361 gcgatagaac ggatcaagag accacggtca gttccacagt cgtcctcacg gatgataacg
    15421 tcttgggcaa cgtcgaccaa acgacgagtc aagtaacctg agtcggctgt cttaagggcc
    15481 gtatcggtca tacctttacg agcaccgtga gttgagaaga acatttccaa taccgacaaa
    15541 ccttcgcgga agtttgaaag gattggcaat tccatgatac gtccattcgg agcagccatc
    15601 agaccacgca taccggcaag ctgtgagaag tttgagatgt taccacgggc tccagagtcc
    15661 atcatcataa cgattgggtt cttaggatct tgattagcaa tcaagcgttt ctcaagtttt
    15721 tcacgggcag cacgccattc agctgtaaca gcattgtaac gctcgtcgtc tgtgatcata
    15781 ccacgacgga attgtttggt gatttgttcg acacgtttgt gtgattcttc aatgatttca
    15841 gccttgtcat caacgactgg gatatcggca atacccactg tcaatcctgc aagagttgag
    15901 tggtggtaac cgaggttctt catgcggtca agtagggcag aagtttctgt cgtacggaaa
    15961 cgtttgaaga tttcagcgat gatatttccg aggtttttct tcttgaatgg agggttgagc
    16021 tcaagattgc tgatagcttc cttgatatct ccaccaagtg gcaagaagta tttagctgga
    16081 acaccttctg tcaagttggc attgtttggt tcttgcaagt atggtagccc ctctggcatg
    16141 atatcgttga agagaatttt accaactgtt gtaagcaaga ccttatgtct ttgctcttct
    16201 gtccaaggct tgttgaggct gtctgttgcg ataccaacac gtgagtggag gtgaacataa
    16261 ccattgcggt aagacataac cgcttcgtca cggtctttga agaccattcc ttcaccttcg
    16321 cgaccagctt cttccatggt caagtagtag ttacccaaaa ccatgtcctg agatggagta
    16381 actaccggtt tcccatcttt cgggttcaag atgtgctcag cagctagcat gaggatacga
    16441 gcttctgctt gtgcttcttc tgaaagtggt acgtggatgg ccatttggtc cccgtcaaag
    16501 tcagcattgt aggcttcaca gacaagtggg tgcaagcgaa gagccttacc atcaattaag
    16561 actggctcga aggcttggat acccaaacgg tgaagggtcg gtgcgcggtt caaaagcact
    16621 gggtgttctt taatcacttc ttcaaggata tcccagatac gctcatctcc gcgttccacc
    16681 aagcgtttag ctgctttgac gttttgcacg atatcacggg caacgatttc acgcatgaca
    16741 aatggtttaa agagttcaat cgccatttca cgtggcacac cacattggta catcttaaga
    16801 gttggaccaa cggcgataac tgaacgtcct gagaagtcaa cacgtttacc gagcaagttt
    16861 tgacggaagc gtccttgttt acctttaagc atgtggctca atgatttcaa tggacggcta
    16921 cctggtcctg tgattggacg accacgacga ccattgtcaa tcaaagcgtc aactgcttct
    16981 tgaagcatac gcttctcatt ttgaacgatg atacctggtg catttaactc aagcaaacga
    17041 gccaaacggt tgttacggtt gataacacgg cggtaaaggt cattcaagtc agatgaggca
    17101 aaacggccac catccaactg caacattgga cgaagatctg gtgggataac cggaaggatg
    17161 ttaagaatca tccattcagg tttgtttcca gacttgtaaa aggcatccaa aacatccaaa
    17221 cgacggatgg ctttgacacg tttttgtcca gtagctgttt tcaattcttc tttgagttca
    17281 gcaatttctt tttcaagatc tacttgcttc aaaaggtctt ggatggcttc cgcacccatc
    17341 ttggcaacaa atgaaccata accatattca cgcaagcgct ctcggtattc gcgctctgtc
    17401 atgatagact tgtgctcaag tggtgtatcc ttaggatcaa tcaccacata agccgcaaag
    17461 tagataactt cctcgagggc acgagggctc atatcaaggg tcaagcccat acggcttgga
    17521 atccccttga agtaccagat gtgagataca ggagctttca attcgatatg tcccatacgc
    17581 tcacgacgaa ctttcgtacg cgttacttca accccacagc ggtcacaaac aattcctctg
    17641 taacgaatgc gtttgtactt accacaagca cattcccagt cttttgtagg accaaagatc
    17701 acttcatcaa agagtccttc acgttctggt ttcaaggtac gataattgat tgtttcaggt
    17761 tttttgactt ctccataaga ccatgaacgg actttacttg gagaagctag ggtgatttgc
    17821 atacttttaa aacgatttac atcaaccact atttcttccc tttctattct aagtgaactg
    17881 cttattcttg ttcagcagct tcttctgttg cttccgcttt tgttgctttc tcagcttctt
    17941 cagcttcaaa ggctgcttta gcctcttggg ctgctttttc gcgggctttt tcaaggtcat
    18001 ctacgtggat gacatcttcg tccattcctt catccaagtc gcgaagttcc acttcttggt
    18061 catcttcgtc taggacacgc atgtcaagac caagagattg caattctttg acaagaactc
    18121 ggaaggattc tggaacacct ggttttggaa ttggtttgcc ttttgtaata gcttcatagg
    18181 ctttcaaacg tccgttgata tcgtccgact tgtaagtcaa gatttcttga aggacatttg
    18241 acgcaccgta ggcttcaaga gcccaaacct ccatctcacc gaaacgttgt ccaccaaact
    18301 gagctttacc tccgagtggt tgttgggtaa cagttgagta aggtccgact gaacgcgcat
    18361 gcaatttatc gtcaaccatg tggtggagtt tgatcatgta catgactcca acagaaacac
    18421 ggttatcaaa tggttcacct gtacgtccat cgtaaaggat tgtcttggca tcgctatcca
    18481 tacctgcttc tttaacagtt gaccaaagat cttcagaact tgctccatca aagactggtg
    18541 tcgcaatgtg aataccaaga gtacgagctg ccataccaag gtgaagctcc ataacctgac
    18601 cgatattcat acgtgatggc accccaagtg ggttcaacat gatgtcgact ggagttccgt
    18661 ctggaaggta aggcatgtct tctacaggaa cgatacgaga gacaacccct ttgtttccgt
    18721 gacgtccggc cattttatct ccgaccttaa tcttacgttt ttgagcgatg taaacacgaa
    18781 ccaacatgtt aacacctgat tgcaactcat ctccatttac acgtgtaaag atcttaacat
    18841 cacgaacgac accatcggca ccgtgtggta cacgaagaga agtatcacgc acttcacgag
    18901 acttgtctcc aaagatagcg tgcaagagac gttcttcagc tgaaagatct ttctcaccct
    18961 taggtgttac tttacctaca agaatatcac cttctttaac ctcagcacca atacggataa
    19021 tccccatttc gtcaaggtct ttgagggcat cttcaccaac gtttggaatt tcgcgagtga
    19081 tttcttcagg cccaagcttt gtatcgcgcg tttctgattc gtattcttca aggtgaacag
    19141 atgtgtagac atcgtccttc accaagcgtt cgctcatgat aacggcatcc tcgaagttgt
    19201 aaccttccca agtcatgtag gcaacgattg ggttttgtcc aagcgccatt tctccatttt
    19261 ccatagaagg tccgtcagcg atgaaatcgc ctttttcaac gacatcacca acttttacga
    19321 gagtgcgttg gttgtaagca gtacctgagt ttgaacgacg gaatttttgg atgtggtaaa
    19381 catccaatga accatcttca cgacgaactt ctaccttatc agcatctgcg taagtaactt
    19441 taccatcata ctgagcaatc acagccgcac cagaatcgtg ggctgcttgg tattccatac
    19501 cagtaccaac gtaaggtgcc tgaggattaa tcaatggcac agcctgacgt tgcatattgg
    19561 ctcccatgag ggcacggttg gagtcatcgt tttccaagaa aggaatacat gctgtcgcaa
    19621 cggcaactac ctgttttggt gaaacgtcca tgtagtcaac aatattagct ggatactctt
    19681 ggttgacccc ttggtgacgt cccatgacaa tcttctcagc aaaggttcca tcttcattca
    19741 gacgagagtt agcctgagct acagtatatt catcttcttc atcagctgtc aaccaaacaa
    19801 tttcgttcgt gacaacacct gtttcacggt caaccttacg gtatggtgtt tgaacaaaac
    19861 catatttgtt caagtgtccg taagatgaca agttattgat caaaccgatg ttaggtcctt
    19921 caggtgtctc gattggacac atacgaccat agtgagtgta gtgcacgtca cgcacttcat
    19981 atccggcacg gtcacgagtc aaaccaccag gtcctaaggc tgacaaacgg cgtttgtgag
    20041 acaactcaga aagcgggttg tgttggtcca tgaactgtga caactgtgat gaaccaaaga
    20101 attctttaac tgcagctgtt acaggacgaa tattgataat ttgttgtggt gtcaagactt
    20161 cattgtcctg aacagacata cgttcacgga cattacgttc catacgagaa agtcccaaac
    20221 gtacttggtt ggcaagcaat tcaccaaccg cacggatacg acgatttcca aggtggtcga
    20281 tatcatctac acggccaagt ccttcagcca agttgaggaa gtagctcatc tcagcaagga
    20341 tatctgcagg agtcaccgta cgaaccttgt catctgggtt agcattacca atgatcgtta
    20401 cgacgcgatc tggatcagtt ggagcaacaa ccttgaattt ttgaagaaca acaggctcag
    20461 tcacaacggc tgcatcgttt gggatgtaga caatcttgtt caagtcgcca tccaaatggc
    20521 tttcaatgct ttcaatcacg ctacgagtca taatcgtacc agcttctacc aagatttctc
    20581 cagtttcagg gtctaccaat ggctctgcaa tggtttggtt gagcaaacgt gttttaacat
    20641 tgagtttttt attgattttg taacgaccaa ctgctgccaa gtcatagcga cgtgggtcaa
    20701 agaagcgagc cacaagcaag ctacgtgagc tttcagccgt cttaggctca cctggacgaa
    20761 ggcgttcgta aatttctttc aaggcttcgt ctgtacgaga gtccattgga ttcttgtgga
    20821 tatctttttc aacagtgttg cgaaccaatt cgctgtcacc aaaaatatca aagatttcat
    20881 catcacctga gaaaccaaga gcacgaacca aggttgtaaa tggaatctta cgagtacggt
    20941 cgatacgagt gtaggtgata tcttttgagt cgctttcaag ttccaaccaa gctccacggt
    21001 tagggataac agttgaacca tagcccacct taccattttt gtctactttg tcgttaaagt
    21061 aaacacctgg tgagcggacc aactgagaaa cgataatacg ttcaccacca ttgatgatga
    21121 aagtacccat ttctgtcatg attgggaaat caccaaagaa aacttcttgg gtcttgattt
    21181 cgcctgtttc tttattgatc aagcggaagg ttacaaaaat tggtgctgag tagctagcat
    21241 cgtgaatacg agcttcttct agcgtgtatt ttggttcctt gatttcatat ccaacaaatt
    21301 ccaactccat tgtgtctatg aagtttgaaa ttggcaatac atcttcaaac acttccttaa
    21361 gaccgtggtc taggaaagct ttgaatgagt cagtttgaat ttcaatcaaa tttggtaagt
    21421 caagaacttc tttgattctt gaaaaactac gacgggtacg atgtttcccg tattgaacgt
    21481 catgtcctgc caagatgatt ctcctttgta aataagttcc aagccttgtc aatcaggctt
    21541 ttctaatcgt catatggttg taaacccctt atcaccgtgt cctcttgacg atttttcaga
    21601 atctttaagc ctctgttaca aatgctcaaa atcttgaaaa aaagcacaaa aagagcagct
    21661 aaatctgact ttttcagaag atttaactgc tgtgagcctt gtctggacaa tatttcagac
    21721 aaaacctacg acaaatgatt acccatatta taccctattt agctagattt ttcaaggggt
    21781 ttcagtaggt ttttggtaaa ttttttccca tagaaaactt ggcatcacat tcgaatcacg
    21841 ctatggtaca aaaaactgaa aaaactattg actgaaaatc attttcaagg tataataata
    21901 aacgttaagg cggtatagcc aagtggtaag gcacggctct gcaaaagctt gatcgtcggt
    21961 tcaaatccgt ctaccgcctt ctataacttg atttatcagg tttcaaatga acagaaagcc
    22021 caatttgaag ggcttttctt attttccctc gaataaatac gtataacttt aaaaactttt
    22081 ggagcgagtt tgtggcagag ttctttccat ggcataattc ccttttgaaa tcaattgaaa
    22141 aaagcttcgt cacgcctggt gacaaaagcc tctagtttat tctgtttctt cttctatttc
    22201 gacttctgtg atttggattt ttaccttggt aacacgtcca ttcttcactt tatcattggt
    22261 taagataaac tgtttgtttt ggctgaccaa ttcatagctg agtttctcag tcgttggaat
    22321 ggttcccact cctgtcaaat aataaccagc gatagtatca acgtcatcac tttccagttc
    22381 aacattaaag taattattga aatcattgag ggtcatagtt ccttgaacaa tgtaggtatc
    22441 ctcgccaatc tgatgaacat ctattgctgc tttgtccgtt tcatcatcaa tttcaccaac
    22501 aatttcctct aagaggtctt ccaaagtcac caaaccagcc ataccaccgt attcatccag
    22561 caagattgcc atttgtcttt gggtatttcg cagttctttt agcaagtcat ccacaaaaat
    22621 agtttcaggt acaaaaagtg gatcttgtaa aattctcttc caaacaatat tgtcaaaacc
    22681 gtccacaaag cctgccttaa ggagactctt ggtgtgaatg attccaatta cattgtcctt
    22741 atccccatca taaaccggga tacgagaata attttgtttt aaaatacttt ggataatggc
    22801 ttgactatca tcctgaatat ccaccataaa ggcatccgtt cgaggaacca taacctctcg
    22861 tgccatcagt tcatcgagcg aaaagacacc ttgtagcatc tcaatctcat cagcatccaa
    22921 tgtttcttca ctatttgtca gcatataggc aatttcatca cgggtcatct tttcatccgc
    22981 atcatcgaat gacataggag tcaaatggct caagaaattg gtcgaagcag ctaaaagcca
    23041 aacaaaagga ctgactagtt ttccgatccc aatgataacc ggcgctgtac gaattgccaa
    23101 ggcatccttt agattaagag cgattctctt aggatataat tccccaaaaa cgatggaaat
    23161 ataggtcaaa aatgccaagg atagaaaagt tgccacggct tgtgctgttt cgccattccc
    23221 aagccaagag gcaatcacac gtcctagagt attagttaaa ctcgcccctg ataagattgt
    23281 aatcagggtg attcctacct gaatggttga taaaaagtgg ttaggatttt ctagtacctt
    23341 cagcaggcgg atgtagcgtc tgtctccttc ttccgccttt tgttcaactc gggcacgatt
    23401 aagagaaacc atggccattt ctgtggctga gaaaaaggca tttaataccg tcaagataaa
    23461 caataaaaca aattgtagca acaaattctg actacttggg tcttccatag ttcttctcct
    23521 aaaatagtat cttatccttt attatatcac aaaatttaat ccaatacata ttattttttt
    23581 cttagcgtaa cttccatctc tctcccaaaa ctggaagtta gtctaagacg gatttccact
    23641 gatttcacgc gtttttctca tactaaaagc taggttgtgg gaaactggaa gttagtttta
    23701 gaagttacta ttttttctta gtacctgttc tagccctgct taaactagta aaaagaactc
    23761 ctacatccat aagtgacata ggagtttagg cttatctttt actgagttac agttacttct
    23821 ccagttcggt aatccagttg aagtcccttt tgaacattgg gaactagaac attgaattcc
    23881 aattctccat ccgaagactt ggcttcaatc tgtgaagctg agggaactaa atcctcgttt
    23941 gaagcgtagt aaagggttac acggtaacgg acacgcttgt tttggtccaa ggctttacgc
    24001 accttgcttt catagtagtt ttgaccagtc gaatactcgg cttgtgcctg atttgcccag
    24061 gctgtctgaa cagcaatgtt tttaggattg cttgttgagg catcaaaacc atccaaacca
    24121 ccgattaagg catagcctaa caaatgacct ctatcgactg catgggtata agagcccttt
    24181 agattcttga cctgatgcca acctggagga gtccaagaag ttgaaccatt cccagtttct
    24241 ttacgattct tgtactgacg agtggcctta gacaagagag cattagctac ggttggaaca
    24301 gtttccttgc ccactgtctt tgttttattg tcagcgtagg gcttacttga aaccttggca
    24361 tctagatttg ttttattacc attgacgata aaagcacctg agccattcca ctccagactc
    24421 ccctttattt gactcttgac tgcgtctgtt aagacactct ctgccaatgc ttgactagga
    24481 gcttcagacg cttgtttttt ctgactcatt tttgtcttag cagcctgatt tcctagttca
    24541 ttctgcttaa tataatagct ccctgcagac aaaagcaata acactagcag tccaatcagt
    24601 gtctgtcttg tttttttgtt catatttctc cttatcttta taaaaaggct ggtctcccag
    24661 cctaatttcc tgtaaattta tgaatcaatt cctgccattt tgccggagac aggatagccc
    24721 acggatcttg acccttgccc aagattccat agcctaccat taaaccgatt cctaaggcta
    24781 gaaggcctaa aatcagtacc agaatgacta aaagtaaacg cttaattacg taacctgatt
    24841 tcttattcat cttcatcact tgcctcaatc cgctgaatct tagcacctag ctgcgccaac
    24901 ttctcatgga aaccgtagta acctctatcc aagtgaacca atttaccgac cacagtttct
    24961 ccctgtgcta ccaaacctgt caaaatcaag gccgcactgg cacgaaggtc agttgaaaga
    25021 acttctgctc cctgcaaagg ctgtccacca acaatacgag ctgtatcacg gataatctca
    25081 gaatgcaagc ccatgcggcg catctcttct aggtgttgga aacgattttc aaaaactgtc
    25141 tccaccatgg ttgattcgcc ttttgcaact gtcatcaagg ctgtaaattg agcctgcata
    25201 tctgttggaa atcctgggtg gggcaaggtt ttcacatgaa cagcttttag attttctagt
    25261 tgagaacgaa cacgaattcc ttcgtcttct tcaattactt caacacccat ttcaagtaac
    25321 ttggcaatca agggacggtt gtgctcccag acagcgtctc gaatcaagac atcaccacca
    25381 gtcatggcag cagctaccat aaaggttcct gcttcgatac ggtcttggac tacattgtga
    25441 gtcgtgccat gaagtttctc aacaccagta atggttatag tctctgtacc agcacctttg
    25501 accttggctc ccatttcatt aaggagaatg gctaagtcaa caatctcagg ctcacgcgca
    25561 gcattctcaa tcactgtcac cccatcagcc agagtcgctg ccatcatcaa gttctgcgtt
    25621 gcaccaacac ttggaaagtc catatagata tgagcgccat gcaagcgttc tgccttggct
    25681 tcgatgtaac cagctgtctg actaatctta acccccatag cttccagacc tttcaaatga
    25741 agatcaatag gacggctacc aatcgtacaa ccacctggca tggatacctt ggcatgaccc
    25801 acacgggcaa ggattggccc taatacaacg atggaggcgc gcatcttgct gacatacttg
    25861 taaggggctt cctcagtgat gtcgccagta gcatccacct tgacaagatg agcttcctca
    25921 tcaaagtcaa ccttggcatt caaaccacca actacctgat tcataataaa gacatccgac
    25981 aaaatcggaa cattctgcaa gacggtcttt ccttcacttg ctagaatagt cgctgccaac
    26041 aagggtaaga ctgcattttt tgctccctcg atcgtcacgc ttcctaccag acgattatcg
    26101 ccaccttgaa ccacaatttt atccatactc gtttccttta ctcttgattt cataataatc
    26161 ctctaagcgc ttcttgccac aaatgataca ggctaataaa gaaagaactc aggatgtagc
    26221 ctatgacaat gctaaaaaag gctactaata aacgaacttt tcttgtatta tcatctgtca
    26281 cttttaaaac cttctcccat ctaacaagat cctttaaaag gtaaaaactc acataaataa
    26341 agaacatatg actacttaga gtgaataata attgaaccat ttgactatta taccatcttc
    26401 tctgtttgtg cgctagtttg gaaacttcac tcaaaaatta gggattgttt ttcaagtaat
    26461 caatcgcatc ttgtagggtt ttaacgggca cgattttcat atccgtcttg attgttttag
    26521 ccgcttctag ggctgtttgg tagttgtttt tcgcgtccgg atgcgccttt tgttcttctt
    26581 cgctaacagg gttatcagga gcaaagaaaa tagcagcacc tgccctagcc gaagctacaa
    26641 ccttcttatc aatacctcca atgtctccca cattaccatc gcggtcaatg gtacctgtac
    26701 cggcaacaat acgaccatta cgaagatctg ggtgagctat ttgagtatag atagctagac
    26761 taaacatgag accagcactt ggaccgccaa taccagctgt tgaaaagcta attgggacat
    26821 tgctgattac ctctgtacgg tcaatcaagc cgattccaat tccatttttg ccattttcca
    26881 aggtgatgat ttttccttct gcagacttgg tttgcccatc ctcttcatag gtgaccttga
    26941 cggaatcccc taatttttga gaactgacgt aatcaatcaa gtctttggaa ctatcaaagg
    27001 tctgatcatt gactgctgtg accgtatcag agatattgag aatcccttta aaggttgaat
    27061 tatccgtcac attcaaaaca taaactccaa agtacttgag ttcgatatcc ttaccagctg
    27121 tttttagtcc ttgatacttg gccatatttt gcgatgtttg catgtagaat tgattgattc
    27181 gcataaattc aacatcggaa gaaccacctg tagtctcctg agcactacga atatctgtaa
    27241 aaggtgtcaa ccaagcataa atcatatgag ctaaagtggc atgttgaaca ccaaccgtaa
    27301 cgaattgata ggcaccagct tccttatctt ctgtgtcatt tactttaagg acttggcgaa
    27361 tatcttccga accacctgga acctctatat aataaggcaa gggcactaca aatgccaaga
    27421 aagtcacaat caaggccgca atgacgtata agggccatct aatctttttt ttcatttctt
    27481 atttcctcca aaatactctc gggaacatag caggcaatat cctgaccaaa cttcaaaagc
    27541 tctctaacgc ctgatgaact gatatagaga tgttcaggtc gactatgtaa ataaatagtc
    27601 tctatatcag aagacagctg atgattgtag taatcaaaac tggcttcata ttgcaaatcc
    27661 gacgcattcc tcaaaccacg cactagacaa gtagcaccca atctttttgc aacatcgacc
    27721 accaattcat catgagaagc cacgacttca acattttcca aatgtcccaa agccttttct
    27781 agcccccgtt tacgattttc aagagggaga aatccttgtt tgtggggatt aaaaaaaata
    27841 cccacacaaa gcttatcaaa aagtctgctc gcccgttcaa tgatatccag atgcccattt
    27901 gtcatcggat caaatgagcc tgtgaataag ccaatcttat ctgacataga ctgtcacctt
    27961 actaattcca taaatctttt ccttccagat acccagacag gcaatttctt ctggaagttc
    28021 aacggcctta tccgtctcgc acacaaccat aacatcttca gaaaaaagct ctctctcagc
    28081 cattttttca atatctgcta cgatttgttc cttggcatag ggagggtcta agaaaacgag
    28141 gtcaaattcc ccagatacct gttccaatgc cctctctgca tccatcttga ggagttgaaa
    28201 ttttccaact tccttggtca tctggatatt ttcagccacg atggtctgag ccttacggtc
    28261 tcgctccacc aaaacagcac tggacatgcc acgcgatact gcttcgatag ataaaccacc
    28321 actacctgca taaaggtcca agactcgtcc cccttcaaag tagggaccaa tcatgttaaa
    28381 aatggctccc ctaaccttat ccgaagtagg tcttgttgtc ttgccttcta gtgtcttgag
    28441 gggacgtccc ccatagattc ctgatacgat tttcatactg tttattatac caaattatag
    28501 acaaaaagag aaagaaaacc gaaccttgcg gttcgattct ctacaaaata ttttcgtaag
    28561 tatcgcggac ttcttgaggc caaacacttg tttgcacttc tccgatgtgt ctcttgcgaa
    28621 gtaggaacat ggccatacga gattgtccaa ttcctccacc gattgtcaat gggaatagac
    28681 cattcaacaa agacttgtgc cattccaatt ccaagcggtc ttcatcacct gtaatttcca
    28741 cctgacgtct aagagtttct tcatctacac gaattcccat agaagacaac tcaaaggctc
    28801 cacctaaaga ctcattccag acaagaatat caccatttag acccttgtag ccattctcag
    28861 actcgcttgt ccagtcatca tagtctggtg cacgtccatc gtgcggttta ccatctggca
    28921 actcgccacc gataccaatc aaaaagacgg ctccaaattc tttacaaatc gcattttcac
    28981 gttctttcgg tgtcaagtct gggtagcgtt ctaccaattc ttctgtatgg ataaaggtga
    29041 tttgttttgg caagatagac tcgatgtcat agcgggcttc aacagctagc tcagtcaggc
    29101 gaatagcctt ataaatcttc tcaactgttt cttttagata aacgatgtta cgcttaccat
    29161 ttgggataac cttctcccag tcccactggt caacataaac agagtgggtt gcatccaagg
    29221 aatcctcatc tggacgaagg gctttcatgt ggacaaagag accctctcct tcaccaaagc
    29281 caaaacgagc caaggtgtgg cgtttccatt tagcaagtga gtgcaccact tcataagtag
    29341 catcagggat ttggagaacc ttgaccgata ctggattttc cacaccagac aggttgtcct
    29401 gcattccgtc accgacctta ctcaagatag gaccttgaac ttcgacaact tctagcttat
    29461 ctttcaaata ctgggtaaaa gtgtttttga caaaggaaat ttcttcttgt tgatggataa
    29521 aacttttctt catagacaac tcctcaaaaa tttaataaac gtattataca cctatttcca
    29581 agaaaagaaa agagaaaatt taaaaaaatc agtgtcactc aaaacactga tttcatagac
    29641 cttttagtca ttgcgaccaa agatacgtag aatacttagg aaaagattga taaaatcaag
    29701 atagatgctg agagccattg acaccaccca gcctgtcgct acacgacctt gtgattgttc
    29761 ataggcgagg cgaatctttt ggttatccca agcaatcaac cctgaaaaaa ccaaaaccat
    29821 ggctacgcta atcatataat caaagaagcc actagccaag aaaatattga ctaccatagc
    29881 aatcagtaaa ccaatgagag ctgccatcat tgctcgacca atcccactca agtctttctt
    29941 ggtaaagata ccaactgccg ccatgacaaa gaagagaagg gcgctcgata caaaggcaga
    30001 taaaactgta cccggagtat agaaagccac cacaaaacta agggtaaagc cgtttaaaac
    30061 ggagtaaagt aaaaatactg gaagagcagc tggactattt cttgaagcca tgctactggc
    30121 aacgaaaacc aaagccaact ccgcaaaagt agcaatggtc aaccagagac gcccctgcat
    30181 caaaaagtaa accaactgag actgaaagac cgtcaacata aggcctgata ccaaagcaga
    30241 tagtccgatt cccagaccaa caaaggcata aaccttagcg taaaattgat tgagacctgc
    30301 acggtcatgt ataatagtgt gattcatcca tttctccttt tctgtgaaca tctttccttg
    30361 attataacaa aaaaagttaa aattagctta aatggaagat taaaataccg gctgcaaccg
    30421 caacattcaa actctcggcc tgccccttca tgctaatatg gaccaactgg tctgcacttt
    30481 cagccataag gggactaatt ccttgaccct catttcccat aactagtaca aaattttcta
    30541 tagaaggcag ttctctgtaa tcaacagaat ctttagatag ggttgttgct agcactggga
    30601 tagctacctt tttagtttcg tcaagaagcg cttgactagt catccggtaa atgggcagat
    30661 ggaaatgact accttgcatg gaacgtaggg ttttgagact gtagatgtct gccgacttat
    30721 ctgaaacaat cactcctgta aaacctgctg catccgcagt tcgaatgata gttcctacat
    30781 taccaggatc ttgcacatct tccaaaaaca agaacttgcc ctgactcaaa tcagcttgtc
    30841 ctacttcttc tctttgaacc acggcaacaa ttccctgtgg agtctgagaa tccgccaaat
    30901 ctagcaaaat atcctctgac acccagacag tttgcgaaaa agtagctaac tgatctcgat
    30961 aattttctag ggtaaagatt ttctcaatcg tcactccagc ttgaacagct tcttcaaaca
    31021 agtgccagcc ttcaatcaaa taggctgact tgcggtattt tttttggtgt aatttcttgg
    31081 tattttttac cacagaattg gcttttgagg ttataatagt catagaaata ttataacaca
    31141 atcaaagggg tttggtatgc aaaaggttag aatgattgcc caaggtaggg tacagggagt
    31201 cggctttcgt tggggtgttt acagcttggc acttgaaatt ggtggcatca caggtcgagt
    31261 atggaataac gacgatggca cagtggaaat cttagcccaa gcagactcat ctgctatcat
    31321 ggcaaaattt atccaagaaa tccgaaaagg accgacacct ttttcaaaag taagctactt
    31381 agatgtcaaa ctaagcaact ttcctcccta ctctgacttt aaaatcgcaa attaggtctc
    31441 cagaactatt gtatattttg taaaaaaaca gtagaataga aaggtataat ttttaaagaa
    31501 ggaataaaaa cagtgaaatc tattaaacgt tttgcattct cggctatggg agtggctatg
    31561 ttgcttgtct tgactggctg tgtcaatgtc gataaaacca caggtcagcc aacaggattt
    31621 atttggaata cgatcggagc gcctatggct gaagccatca agtacttcgc tactgataaa
    31681 ggtctaggct ttggtgtcgc tatcattatc gtaaccatta tcgtacgctt gattatctta
    31741 ccacttggta tctaccaatc atggaaggca acgcttcact ctgaaaagat gaacgctctc
    31801 aagcatgtcc ttgagccaca ccaaacacgt ctcaaagaag caactactca agaagaaaaa
    31861 ctcgaagccc aacaagccct ctttgccgct caaaaagagc acggcatcag catgtttggt
    31921 ggtgtaggat gtttccctat cctccttcaa atgcctttct tctctgctat ctactttgct
    31981 gcccaacata ctgaaggggt tgctcaagca agctacctag gcattcctct aggttctcca
    32041 agtatgattt tggttgcctg tgctggtgtc ctttactatc ttcaatcgct cctttcactt
    32101 cacggagtag aagatgaaat gcaaagagaa caaatcaaga aaatgattta catgagccca
    32161 ctcatgatcg tcgtcttctc cctcttctca ccagctagtg tcacacttta ctgggttgtc
    32221 ggtggtttca tgatgattct ccaacagttt atcgtcaact atatcgttcg tccaaaactt
    32281 cgcaaaaaag tccgtgaaga actagccaag aacccaccaa aagcaagtgc tttctctaaa
    32341 ccaagtggac gaaaagacgt tacccctgaa caaccaactg ctatcacaag caagaaaaaa
    32401 cacaaaaatc gcaacgctgg aaaacaacgt tcgagataag aagaaaaagg cttagctgat
    32461 ttgctaagtc tttttgcaaa tcaaaagccc gatgtttcca tcaggcttct tttctatcca
    32521 tgtacacgtt tcatataatc ttgataactt tcggtatcca tgagtttttt agcgttcttg
    32581 acgcgatctg ctgttggtgg tttgactcct tcgagggaat atggaattcc aagttcacgc
    32641 cacttgaact cacccatggt gtgataaggt agaatttcaa acttatcaac atttttgagg
    32701 gtcttgacga acttaccaag ttcaatcaag tcatcatctc tgtctgtcaa tcctggaact
    32761 agcacgtggc gaatccagac aggttttcca atatctgata gatactgggc acaagccaag
    32821 atatttttat tagtttgtct agtgacaatc ttgtgctgtt cttcgttgat ttccttgata
    32881 tccaaaagaa ccaagtcagt gacagccatg agtttgtcaa acttctcaag gtaacgtggt
    32941 ttattacgga aaggaagagc acaggtatcc aaggtacagt ggattccttg ttccttagcc
    33001 ttggtgaaga gagcaatcag gaaatcaatc tgcaagagag cttctcctcc actgactgta
    33061 atcccaccct tatttcccca gaaaccacgg tagcgcaagg cctctgtcaa gacatcatct
    33121 accgtccgtt cacgtgactt attggactcc atagcccaag tgtctgggtt gtggcaatac
    33181 tggcaacgca tgtgacagcc ctgcaaaaag acaataaagc gaataccagg cccatctact
    33241 gacccaaagc tttctgtcga atgcaccatt cctgtcactt gtccataatc aattgtttct
    33301 tcagacatat cgttaccttc cttgaaaacg ttttatagtt ttattatatc acgacttgaa
    33361 ggaaaaacaa gatttttgcc tattttttag aaagcaatct gtttttcaat ttcattttca
    33421 atctattatc agattattct tcaaaatcga agccatataa ggttgcaaaa tagtcctcag
    33481 ggcgctctgc acggcggatt tgacgggctt taccttcttc ggtatagagg atttccgcag
    33541 aacgtaattt ggcattatac tggtagccca ttgaaaatcc gtgggcacct gtatcatgaa
    33601 ttaccagcaa atcaccgatt tctgtatgag gcagttcgcg attgactgca aatttatcat
    33661 tgttttcaca gagtgaacca accacatcta ccgcttcagc tggtccatct ggatgggtca
    33721 cgttgctaat atgatggtaa gctccgtaca tagctggacg catgaggttg actgctgagg
    33781 catccacacc tagataggta cggtaggttt ccttcttatg agtcactctt gtgactagag
    33841 caccgtgagg tgccagcata aaacgaccca attcggtgaa aatcttgacc tgaccaagac
    33901 ctgctgacgt aagaacttct tcatacacct tacgaactcc ctcaccaatc aaggcgatat
    33961 cgttcggctc ctggtctgga cgataattaa caccaatacc gccagaaaga ttgataaagt
    34021 ctagccaaat gcccaacttt tccttgattt caacagccag ttcaaagagc tgacgagcca
    34081 actctggata atagagatgg gtcacggtat tggacgctag gaaggagtga atcccaaaag
    34141 tcttggctcc tttttccttc aagatagcaa aggcttcaaa gagctggtcc ttggtcatgc
    34201 caaacttagc ctccccagga ttgtccataa tgtctgtccc cagttcaaaa acgcctccag
    34261 gattataacg acaagagatg atttctggaa tgcctgctac tctctccaga tgttcaatat
    34321 cttcaaaggc atccaagtta atggtcgcac ccaattcacg tgcataggcg tattccttgt
    34381 ctggcgtgtt gttggaagag aacataatct cagaacccag aaagtccagt ttatggctca
    34441 tcaaaagctc tacataacta gagcagtcca caccacaacc ttcttcttgg agaattttca
    34501 aaatagctgg agttggagta gccttaactg caaaatattc cttaaagccc ttgttccacg
    34561 aaaaagcttg gttgacggct cttgccttct cacgaatccc cttctcatca tacaagtgaa
    34621 agggagtcgg gaactcggca acaatcgctt ctaactcttc tctattgata aatggtgttt
    34681 tcataagctt ccttctattc tgtttgcaaa gaaaggctgg gacaatcgtt ccaaccttta
    34741 gttctatctc ttatttatct tcgtcaaaga tattgccaac ctcaacatcg atggcttggt
    34801 tcttgacatc aatattggtt accttcaaga gtgacttgta gtcaaactgc ttttcacgtt
    34861 cttcttgggc attgtccgca aagaccgcta cacctgccag ttctgagtcg aactcgcgca
    34921 agagactaat cataccattg accgttccgc cacctttcaa gaagtcatcc acaatcaaga
    34981 cacggctgcc tgccttaaga ctacgttttg aaaggaacat tttctcgata cggtcaccac
    35041 ttgaacctga aacatagttg acgctaacag ttgaaccttc ggtaattttc aggtcacggc
    35101 gcacaatgac aaaagagaca ttgaggacat tggcaactgc atttgcaagt ggcacaccct
    35161 tagttgctac ggtcataacc gcgtcaattt tttggtccat aaagcttttg gcaataatac
    35221 gaccaatatt tttcaagatg gctggtgtgc taagcaaatc agacagatag atataaccac
    35281 ctggcaagat acggtcactt tctgacaact tggtacgcaa gtcttcaacc atttccttag
    35341 catcctgact cgaaatagac ggtgtgaaga tgacacctcc gccagcccca gtcactgtct
    35401 ggatatgacc gatttcaatt tcctcaaagg cgcgtttgat aatgacgata tcttctgaga
    35461 tggatgattt agcagactca tacttttcag caaaagtatt gagactagtt agtttataag
    35521 gattattaat caaatagttg gaaatgacaa ccatccgatc acttcttctt aatttcattt
    35581 ctatttccta ttccattttt attcaaaaaa tcaaaaagca aactagaaag ctggtcgctg
    35641 gtggttcaaa acactgtttt gagattgtca atagaactga caaaccctgt aatatacctg
    35701 catatataca tacgacaagg cgatactacc ctagtttgaa gagattttcg aagagtattc
    35761 atttttgtct tttacttatt ataccatatt cacataaaaa aacgaacatt cttatcctaa
    35821 aaaatgctca tttttcttaa attatcaatc taaatctggt ttatagaagg aacgattatc
    35881 catagcgaag attttattgg tcatctctcc tttatccacc aaagccagag ctgttgacat
    35941 catcatcatg cttgcatcca gattgtcaat catatggata atctctgcct ccataatacg
    36001 tggacggact ggacttccat attcaagcaa gccgtggtga ctgaggatga catgacgaag
    36061 caaaacgact tcttccttgg tatcatcgat gccgagttcc ataactgtct tggtaatttc
    36121 gctatcaatg agagcgatat gtccaagaag attacctcgc actgtgtact ctgtctggtc
    36181 tggccccgtc aactcgatga ccttagccaa gtcatgcagc ataatccccg catagagcag
    36241 gctcttattg agctgaggat aaacttcgct aatagcgtct gccaaacgca ccatggtcgc
    36301 cgtatgatag gccaagcccg tttcaaaggc atggtggttg gtcttggcag ctggatagga
    36361 gtagaattcc ttatcatact tggtgtagag atttcggaca atccgttgcc agacaggatt
    36421 ttcaattttg aaaatcattt gcgacatgta gtcacgaatt tccttgacat caactggtga
    36481 cttgaccttg aaatcagctg ggtcattggg ttcaccagct tgaggcaggc ggagagtaat
    36541 ttgattgact tgaggggtat tgttataaac ttctcggcgt cctttcatgt gggcaacctt
    36601 acctgcggta aaggcctcaa tgttatgagg ttgggcatcc cagagcttcc catcaatctc
    36661 gccactatca tcttggaagg taaaggctag gtagtttttc ccagctcgag tttgcctcag
    36721 gtcagctgat ttgattaggt aaaagccttc aaataactca tcttttttca tgtgactaat
    36781 cttcatattc ttcctcattt tcttgaaaat ggagtagatc aagcgcaggc tcaccttctg
    36841 acaactcaat gtgacggagc gtccgctcga tagctatggt acgacggttt aataattcat
    36901 caatattgcc agaggcatgt tggagatgtt tttgtgcctt gaccagaata ccaccaaact
    36961 tgccaaactc ggtcttgaca ctggcaagag tcttgctgat atggtcggca ctcttttgga
    37021 tattaagggt cttgaaacca actgataggg agttaagaag ggctgatagg gtacttggtc
    37081 ctgcaacaat aatctgttct tcccgtctca aatcatcaaa gaagaccgga ttgcggacga
    37141 tttctgagta gagaccttct gtcggaacaa acaaaactcc aaaattggtc gtccgaggtg
    37201 gtgctatgta cttgttccta atatccctag caaagcgctt gacgcttgct aggagtgact
    37261 tacgacagcg ttcaatctca tccttgtcac ctgtctcata ggctgcttcc aagcggtaat
    37321 aatctgccag tggaaactta gagtcaattg gcagatagac gtattcttgg tcgccttgtc
    37381 cgggtaactt gatggcatac tccactcgtt cactagagtt ttcaaccgtt gcgtattctc
    37441 gttcgtactg ggcaggtgtc atgatgtctt caataatttg ccccagttgc aattctccca
    37501 gaatccctcg cgtcttggtt ccagagagaa ccttgttaag agctccgaca tcacgggcaa
    37561 ctgtctgcat ttctccaagg ccacgattga cagactccag ttgtttagaa actgtctcaa
    37621 aggaagcctg taagcgtgtc tgcaaggtct tttctagttt ttcctcgacc gtctggcgca
    37681 tttgttccaa acgttgctca tttgattcct gcaaggcttg gagacgttgg tctgtcttgt
    37741 ctctagtttg gaggagatta tctgtcattt cttgacggac ttgggtcaga ccttggtgca
    37801 attcaatccg cacttcttgc aaacggtcgc tgacaaccac ttccaaatct ttttggtcta
    37861 actggctggc ttgtctggct tggtcaaagc ggtaatccaa ctggtctgac aaatgatctg
    37921 cctgatcctc caagctctta cttaagtgtt tctcctgcct atcctgcctt tgccaaatca
    37981 gaaagagacc agctagattg gcaattaata atagaataag taaactttcc atcctacctc
    38041 ctgtccttgc tatgcagtac gaccacataa ccatctgggc aagttaccga cacttcccta
    38101 tctatatatt cgttagaagc gtacactttt ttaaagaaaa aattttcctc tgtcaactca
    38161 tacttggctc caagaatagt cagctggcta tcccgaactg gcataaaggc tagatagtcg
    38221 tagtctgaac gaggttctag ctgactgatt ccttctggac aataagtaat caagttttgc
    38281 ccatcctcaa tttctatttg atgcatatag ggtgccaact taggattgct aggcagaaag
    38341 acattggcca acatatggtc aatacggcca cccaaggcac cgaaaatagt gacctgagcc
    38401 tgaggatttt gttcaaagat ggttaagaga gccaattcca gatctgtatc atccttttct
    38461 ggtcgtgctt ggacaaaata ctgggcacct ttttgaatca cctgtcgctc ttcttccgtc
    38521 acagaatcaa aatctccgac tgctagagca agaggtaagt cttcttccaa gacccagagc
    38581 gagcctcgat ccaccccaac aaaagcatca aaatctgtcc gataatgacc gcggtttccg
    38641 cctgcaaaaa ctgcaaccct agtccagttg ttttctgaga gtttgtactc gctcattgac
    38701 ttctccctta aagacatagg aacctgctac aaaaacagtc gcaccggctt ctttggcttg
    38761 agcaatagtt tggtcatcaa tcccaccatc cacttcgatt tcaaagttca aacctttttc
    38821 ctcacgaaga gcaaccaact cacggacctt atccatggtt tctggcagaa aggcttgccc
    38881 accaaaacct ggattaaccg tcatgactaa aacttggtca actagatgaa ggacgtgctt
    38941 gatggcttca actgatgtgc caggattgat aacgactgaa ggcttaactc cgagtgaacg
    39001 aattttttgg agggcgccat gaatatgagg cgttgcttct acatggatac tgatgatgtc
    39061 tgcacctgca cgcgcaaaat cttccagatg atgctcaggg tttgacacca tcaagtggca
    39121 atcgaaaacc atcttactat gaggacgaag gctctcgacc acacctgcac caaaactgat
    39181 ttgcggtaca aaatgactgt ccatgatatc gatatgggca tattctgccc cagttgcttc
    39241 tagacgtttg atttcacgtt caaagttggc ataatctgct gccagaattg acggagcaat
    39301 cttgtattga gacataggtt tctccttatt ttggaatttt tttgctgact tttttatagg
    39361 tttctctacg attttcaatt tcactaagga attgcaggta attgtcaaaa cggaaggttg
    39421 caataacacc ctcttcaaca gctggtttga cggcacaaga cggctcatgg gtatgggtac
    39481 aagtacggaa cttacaatct cggctaacag tagcaatctc tgggaaagcc tgattgaggt
    39541 cttcagccct tgatacttca tagtccaagg atgaaaatcc tggtgtatct gcgattttac
    39601 ccccgttgag attgtaaaaa ctaacagctc gagtggtatg gcgaccgcga cctagactgt
    39661 ctgaaatttc tcccgtttca agattgaggt ctggtgcgat tttattgaga agagttgact
    39721 tcccaacacc tgtctgcccc ataaagaccg taaccttgcc tgttaacaaa gacaggagtt
    39781 cctctttact ggtcacaaag tcatagccga tgtcaccata ggtctgctgg taaaaatcca
    39841 gttctcccct atcttccaac aaatccattt tggaaatata gacaatggga tggatgccct
    39901 tgtgctccaa aagaaccaag aaacgatcca gcaaattgct gttaaaatca ggttccttga
    39961 cggacatgat tactacagct tgatcgatat tgacaatagg cggacgaacc agactgtttt
    40021 tccgttcgtg aattttgagg atatagcctt ctgaattttc ctcggcagag aaatctaccc
    40081 agtccccaac ataaggggta tggccttttt tacggaaatt cccacgcgcg cgtgtttgat
    40141 aaacctggcc atcactctcc acatagtaga aacctgccaa ggctttaatg atttgtccct
    40201 gcatcttaga gtccttgccc tttaagtgcg tctgctagac cggcaaattc tgccaagctg
    40261 agagcttccc cacgcacact tggtgacaag cctgcctggt ccaaagcctt ggtcagcttg
    40321 tccttgacct cttcagtctt accaaagtaa cctgtcaagt tattccacaa ggtcttgcgg
    40381 cgatgggtaa aactagcctt ggaaacctta aagaaaaagt tctcatcttc tactgctacg
    40441 gctggctctg gacgacgcac cattttcaag atggctgaat ccacatttgg cgctggcaca
    40501 aagaccgtac gaggcacgat aaaggcaacc ttggctgtca tgtaatactg cacggcgata
    40561 gacaagctac cgtaagcctt ggtgttaggc tgggctgaaa tgcggtccgc tacttctttc
    40621 tgcatcatga ccacaaactc acaaaaagga atgccactct caatcaagtg catgagaata
    40681 ggcgtcgtga tgtagtaagg caaattagcc actaccttga ttggcaggtc aggattttta
    40741 aaattctgga tatgttgcgc caaatcaacc ttgagaatat cttcgttaac tacggtcaca
    40801 ttatcaaaat cacgcagggt atctgccaaa attggcacca aacggtggtc aatctcaaaa
    40861 gccatgactt gggctgcacg ctcagccaaa aattctgtca aggcaccaat acctggcccg
    40921 atttcgatga cattgacctg atcatcaatt tcagccgtat ccacaatttt ttgaaggata
    40981 ttggtatccg tcaaaaaatt ttgcccaaag gactttttaa aggtaaaacc gtgacgctcc
    41041 agcactgcct tggtcacgct ataatctgca attctcatct attctctttt ctattatctg
    41101 ttactactat tgtaacacat tccccttgta ttttgggaat gaaaatcata ccaaaaaact
    41161 atctttcctc atgaagaaag atagtcttgg aaatctttaa aagtggcctt agcagttctc
    41221 tcgaaacatg cgttcaaaaa aatcagcttc ttgataactt cgattttcag gtagggtcaa
    41281 atccacatcc tcctccacat catccactgt cagttcaacc atctgaggta aatttttcaa
    41341 acgataatgc ttttgccttt gcccttcaat actagcaaaa tcaagcaaat agccatgcac
    41401 tcggatttga accaaaagga gatgattggt catattttcc atgatgagga tatcttctaa
    41461 accacctctg gcactaggat aatcagatac cctgcgaata cggatttttt cacacatgag
    41521 caagtttcct catatcaatg atttcatcag ccttattcca gactagggga ttatgggtcg
    41581 caatgatgat aattcgattt tcatctttca aatccaccaa gagattcata acctcttctg
    41641 aattttcagg atcaagagct gctgttggtt catctgccaa aatcaagggt ggatttttca
    41701 aaatagtctt agcaagggca actcgttggg cctctccccc agataaagta tagatttttt
    41761 gttctaaatc caaataccct agattaactt tttctaaagc ccccacttgc ctttccaaac
    41821 gttctacttt tgagattttc tgaccaacaa aacccaaatc caaattttct ttgattgatt
    41881 ggttttctaa gaggccgaaa ttttgaaaga gatagcccat ctggtctcga aaatactcac
    41941 gagtgggaat ggtttttaaa tctttcccct gatagagaac ccttccacca tctatctttt
    42001 ctagctttcc caagatattc agcagcgtcg tctttccgct tccactcttt ccgattaagg
    42061 cataaacctt gcccttctcc agcttgagat tcaaaccaga gaaaatcgcg cggtcattaa
    42121 atttcttttc caatccttga atatcaatca tcttattttc ctttcaatac tgccatgcta
    42181 ccagcttctt cttttttgtc ctgccttact aaaatcaaga gggcgttaag cgtaaacaaa
    42241 gctacaacta gagcgctaat caaaccatct cttgtcaaaa tactagatag gactaggcca
    42301 agcaagagaa ctcctccttg cgccagtaaa tacttgccat gaagctcata gattgtcata
    42361 ccagcaagac gtttaatcat aagttcccgt ctgaactgct caaaatagag aagattcatg
    42421 gaatcaaata acaagatagc cgtagacagg gtcaaaatcg tcccaataag gagagagtaa
    42481 aactccaccc gtttgtcatt tagtaccttg gcaaaaaata gctggctttt taccaagtaa
    42541 gaaaccttgt gatacagacc tttctctttt agagctgtga tgctgtctcc atatcgatct
    42601 agtttcaagg cactattagc cgtagttccc cacaacatcc ctgcaacagg tcttgttcca
    42661 gtcgcttgcg gcgttagaac tacaataatc ggatctttta ggtactggcg tgttgtcttg
    42721 tacccatcat tatagaggaa acgttcctgt cctgtttctg taaaagctag ccttacctgt
    42781 gggaggtagt gtttctgact cgtcacttct acactttcag atgaaaagtt ttgcaggtaa
    42841 tctgtaaaca atccttggta gtagacagac tgctctcgca agctctcagg caagatcagc
    42901 ccaaactctc cctcagacaa gttttgcatc ttgtccataa actctgaaga aacggtaatc
    42961 ttttcttcta tcagatagcg tggtgagaca tagataacat tccctgacgg tgtgtagtca
    43021 ctgagacgat tgccatctag gtccacttct gctccatctg agaaattgtc aacattgctc
    43081 ataatataaa aagagtctgt attggctaac cgttcttcag taaatgtctg ccactcacga
    43141 ttatccttac gcgttccttc ttcatcggca aatgcactag accaaccaaa ggatagacgg
    43201 taacggtctg aggactggct ccatttattg ctagctctct ccatttcctg catttcacgg
    43261 tagtggggta ggagagctgt cgcactcgat ccgaccacca atacagctaa gagttgcccc
    43321 accatcatca atgtcattat acgtttgaga gggagtttcc ctttcaatag atccaccaga
    43381 ctattttcct gtaaaccaag tagatagacg acacttagta aggtagaaat ccctgccagg
    43441 gtcaatccat aaagtagaag agcaatgatg accagttgca ccgttgccat aaacaaggca
    43501 ccttgatacc agagaatccc caatcccaaa agactggata ccagcactga gcagataagc
    43561 tgtctcacat cttctaacac tggtctgaga gcaactccaa acaagctctc accagctatt
    43621 aagcgaatcc ctgcctgacg aagggatttg atccgataaa tcagggtcag actcataaag
    43681 gtcagcagaa aaatagctaa actcagtagc accatagggg tggccgtcaa tagtaagact
    43741 atcgaaaatg gatcttctcc attcgaaaca aagccttgat aaccaagctc tttcaaggtg
    43801 gtctgtaagc tcactccatc caaacttcct gatacaatca agtaagaccc taataaatca
    43861 ctcgtttctg cactctcctt ggaggaaatg gtcagacctt ctggaagctt tccctcacca
    43921 taagtggcat aggtaaagcg tgtttctcca gcttcatttg gctcaacgat tcgacgagca
    43981 atgagactat tgtgctctac cgccaagtga ctcaaagcct ctgtcacctc ttcgcgacta
    44041 gctactggtt gatttatcat cattcctgtc acatcaacag aaggatagtt accaaataaa
    44101 atctcttctc gatggtcaac ggaagttatc accatataaa gcgatactaa taccattgaa
    44161 atcaaaataa ataaacgttt catcatttcc tccaaaaagt tacttggtca cttagtgaga
    44221 aggtgaccaa gtaaagctaa acaatagggc tataccattc taaacattat agtagaatgc
    44281 aactttctct ccccaagaag tacgaatcca cgcacgagaa gtccctccag caccagcata
    44341 tcccttgtca gatgaaccag tttttctgct aacaactgtt gaactatggt attttgaagg
    44401 gtgatagtag tttgagtaag caccaccgtt acctgaacca taagtccaaa caccaccttc
    44461 tgggtattga acggctgcag aggcaactga tacacctcca cctaatgttg ctgcaatgct
    44521 cgctacaaga gctaactttt taactgtttg tttcatattg atactcctct atttttgatt
    44581 acaagattgt tgagttactt gctcctcttt ccattatcaa cgttgatact attatattac
    44641 caaatgatat tctaaaatta tttgtttttc tctaaattgt caaaatagca ccctgaaatg
    44701 aacttttata gtcattatac tccatcttta tcacactatc aaggacatca atgtccttaa
    44761 aatctaacta tttcatatta tttttagctt atatctaaaa aaacagttct aaagacgaac
    44821 tttagaactg ttttgatacg aggacaagga catttttgtc actgattcac tatgtttata
    44881 aatatttttt gacatcctct tgccattcct ctgtcaattt ctttactaga aatccatctc
    44941 caattagctt cgcaaatgtt ttagaagact gataaaatga ttcagctaaa ctaaaatctc
    45001 tttctactct taatgcttgc ttccacaaaa acataaatac aagaggtttc ttttgataat
    45061 cttgagtttt atccataatt tcttgtaaac acgatagata atcattaaaa caatcagtta
    45121 ctcctacctt ttcaagacaa cacattcctg aaataagagt atctctaatt ataaagtaat
    45181 tctctatttg agttaccttc ttctgttcca aaagaaagag cattagcttt tcgatttgat
    45241 ttataaattt ggcaagatct tttctaatga gcatttgata aaaaaacaaa cgaatcaaca
    45301 acatatcatt tattgaataa gcttcttttt ctataatctg aggcatatgt tcctcaagta
    45361 ttaactctgc atattcaggc cttctagttc tagaagtatc caaacacgcc tgcaatactt
    45421 cacatgctaa ttgttcttct ttaggaagat tatcaaagta tgttttataa atctcttcca
    45481 aacacgcttc cttcttatcg tactcctctt ctttaccata gattggttca cgtaaaatct
    45541 ggtatttcaa ttctaaataa gcagaaggaa gagctgaaaa atccggcata aggctataaa
    45601 ctggaactcc tagccggcga gcaatatagg ctaacttggg caaactcggt tgcgaagctc
    45661 cactttcaat tcgcgacagt tgacgaactg tcagttcttg ctcatctcca caaaaatctg
    45721 gacgactaat tcctttttct atccgaatct ctttaatttt ttgaccaatt aacatcagac
    45781 gcctcctctt ctacctgctt cgaaaaatac tgtgaaactt aaaaaattgt aacggctaca
    45841 tctttcctac tcataagatt tcatagcttc ttccacttct gccaaagtaa ccccaaacaa
    45901 ctctaggcgt ttgaggagtt gcttgccgtt ggaatagccg attcggagag tctctccgag
    45961 atattctcta cgcttacggc tgtctgcccc tgctagaaaa ccaaggcgaa tcaagtcgct
    46021 acgactaatg tcaaactgac tctcatgttc aaattgttct gtcacctgag ctagagccgt
    46081 tttcaggtct tcatagctgg catgctcaat tcccagagaa cgccccttgg tcttggactt
    46141 gggaacagct tcatctcgct tgagaaaggc atgctgaact gttggaatgg ccgtcataat
    46201 catgcgccga atccgctccc cattaaaatc tgggtctgta aagacaatga ctccatgacg
    46261 ttggtgtaga cgctgaattc gctctatatc ctgatcattg atggcagaac ctcgtgtctc
    46321 ataggtctcc acatcgaaat aacgcttgag attgaccgta tcatcgcgac cttcaaccac
    46381 gataacttga gaaattttct ctttcattac ttgctgtcca atccaaaaat tcgttctgca
    46441 tttgcagtcg ttgctaccgc cagctcttct gtcgtcatac cacgcaagtc agcgataaag
    46501 tcgaccacat agcgagtata ggctgtttta ttttcacgac cacgcttggg tacaggtgct
    46561 aagtaaggcg catctgtttc caccaaaatc ttgtccaaag gtaactcttt agctgcttct
    46621 tggaggtcag ttgccttctt gaaagtcacc actcctgaga aggaaatggt cataccaaga
    46681 tccacaaact tctctgccca ctcaagcgtc cctgaaaatg aatgcatgat accaccacga
    46741 ggaccaacgc cctcactctt gataatctca taggtatctt ccagcgcatc acgggtatgg
    46801 acaacaaaag gcaaatccaa gtccttagat agctgaatct gacgacgaaa aacctgctcc
    46861 tgcacctctt tgggcgctgt catccaatgg tagtctaagc caatttcacc tagagccaca
    46921 accttgggat gttttaactt atccaacaag taagcctcaa cttcctctgt ataagtacca
    46981 gcttctgtag gatgccaacc aatagtcgca tagagctgct catactcatc taccaactcc
    47041 aaggcacgct caatcgtcgg tttatcaaaa ccaacaatat tcatctgtat cacacccatc
    47101 tcagcagcca aggcaatttc ttctgcctca cgacctgcaa attcttctac attcaagtgt
    47161 gtatgtgtat caaaaatcat ctcttctaac ctcgttttct atcttctatt ataccaaaaa
    47221 agaggaggag cacctaattt ttcggtttcc cctcctctct tcaatagaga gctattctgc
    47281 tatcttttct atccgatatt gcccatctcc tattccacag ttagagacag aagagattgg
    47341 ctacattgat agaatattcc tattcagcta aagtatgtga ttatcttggg ttctcacttt
    47401 tctcacccat cactaggatt gatgacttct caaaatcccc cttataatct aactttaagt
    47461 agagaataga attttctagc tgttctattt tctacgtcta gtctttcttc ctagcaaagc
    47521 aaaaccactt gttgccacca ctagaccaag actagcaagt gctgcacttg atgctgaacc
    47581 tgtgttaggt aattgtttgc cagcaggtga ctggtaggta acatcttgca ttgttcctgc
    47641 agttgcttgc ggtgcacttg ttgtcttagg agcagcatta ggagccacta gtctcttagg
    47701 aactgtaacc atggcaatgg cttgcactcc aatcgtttct gcttccttca catcttccat
    47761 agttttcgca ttttcaatct ctttgagagc tgcttgtttt tctgcttcca ctcttgctaa
    47821 aagttctgct ttttctttat cagaaagcgg tgcgcctttg atttcatcct gcttgtcctt
    47881 ggctgctttt tcaatggcat ttttagctga ttctttttca gtagctaatt gcttagaagc
    47941 ttgtgattga gctgagtttt gtgaacctgt gtgagctgat ccacccgcct ggctaccacc
    48001 gcctgcattc gcattagcat ctgagcttcc agtattgcct cccttatctc cacctggtgt
    48061 attttctagc ttatactctg gtgtagcttc ttgagttaca gattcacctt tcgcagattg
    48121 ttgaactgta tcttttccta gaatcgtcac tactgaacca tctgggaatg tgattgttgc
    48181 tgtaccatct ccagctacat tgattgtcgc tccgtctaat gctgaaccat ttgcttgtaa
    48241 aatagcaacc ttaacttttt cttcatctgt taaatgagtt atatctttta catctactct
    48301 ttgagcaggt gctttaacag ttgatttttc agcagtcggt ttagtgctat cttgtgcttt
    48361 tggatcttgt gctaccaatt gatttcctgg aattgtaata gctgaaccat ctttaaagac
    48421 gattgtcgct gtgccatctc cagctacttc aactttatag taaaatgaaa taataacagg
    48481 acaaatcgat caggacagtc aaatcgattt ctaacaatgt tttagaaata gaggtgtact
    48541 attctagttt caatctacta taacatcgct tccgaatcca tttgcatcta ctgaactttt
    48601 ttacaaaaat aaaaatcagt agactcgagt tcctactgat ttttgataga attcgtttgg
    48661 attatgcagc caaaactttg cgtcctttac gacgacgagc tgccaatacg cgacgaccat
    48721 ttttagttga catacggtta cggaatccgt gtttgcgcgc acgacgaagt ttacttggtt
    48781 gataagtacg tttcacgatg aatacctcct catagatttt gtattcgttt agccggctat
    48841 aaagtgatca gttactaaac atactttact attctatctg attctttcac ttttgtcaat
    48901 agctctaggc aaatgtttcc agctttttcg aatgcaagcc ctatctacga tactgtttca
    48961 agaaacgagt catcttttct tggatttggg ctaactcatc aacacgagga aggtaaacga
    49021 tacggaagtg gtctggttcc tgccagttaa agcctcgacc atgaaccaag agaacctttt
    49081 cctgcttcaa gaaatcaagg acaaactgct catcatcatc gatacggtac atattgcggt
    49141 cgatttttgg gaagatatag agccccgcct tgggtttaac ggcagacaaa cctggaatat
    49201 cttgaatggc attatagatg aaatttcttt gctcgtagat tcgtccacca ggaagaagca
    49261 attcatcgac tgattggtga ccccccaagg aagtttgtac gacttgttgg gccaaaacgt
    49321 tagagcaaag gcgcatattg gacagcatat tgagcccttc gatatagccc ttaacatgag
    49381 tcttagggcc agacaagacc atccaaccca cacggaaacc tgctatgcgg tgggattttg
    49441 acagaccatt catgctgaca cagaagacat ctggtgccaa gctcgccaca ggcgtatgca
    49501 catgtccgtc cattaccatg cggtcataga tttcatccgc aaagataatc aaatcgtttt
    49561 gacgggcaat ctcaataatc tccagcaaga gttccttagg ataaagggct ccagttgggt
    49621 tatttggatt gataaggaca attgccttgg tattggaagt gatttttgac ttgatatcgt
    49681 caatatctgg gtaccattcc acagcttcat cacagatata gtgaacggca tttcctccag
    49741 ctaggctgac agccgccgtc catagaggat agtctggcat tggcactaag acctcatccc
    49801 cattgtccaa aagcccctgc atggacatga caatcaactc actgacacca tttccaaggt
    49861 aaatatcatc aatatctacg tttgggaatt tcttcagctg gcaatactgc atgatggcct
    49921 tacgggctga gaaaatccct ttggagtcag aatagccttc actatcacgc gcattcataa
    49981 tcaagtcatg aatgacctcg tctggcgctg taaagccaaa ttctgctgga tttcctgtat
    50041 tcagacgtaa aatcttttct ccattcgctc gcatccgcat ggcttcttcc aatacaggac
    50101 cacggatatc ataagcaaca tgctctaact tactagactt gttatattct ttcatcttgt
    50161 ttcctcaatt catcaggata gtaacattat accactagaa agagaatgcg acaagtttac
    50221 tgaaatgtgt tactagtttc gtatagaaga aaaaccttgc taaaggcgag gttaagaacg
    50281 tagagatagc aggtattttg caaagacaca tcacatacca aaagataaac ctagaaaaag
    50341 caagcctaga aaggagtcat aaagaagatt aaacatgagg aaaagccccc atatcatcaa
    50401 gtaatcccaa cgcccacttc gttttgcaac tagaaataag attagatggt agagtacatg
    50461 aaagactaaa aatccaataa aacttggata aagaagcggt accagactct ctaattgcca
    50521 gatcatgatc acttctacca agactgaaac taggataatt ccataataaa ggaaatttga
    50581 tttttgaaaa gagtttttca tcattctccc ttccttcact tctttcctgc tatcctttct
    50641 tttattttat catatattta tatagatttt aagccctaat attcttttga aaaatacttt
    50701 ttttgcttta cttctatagt gaaaaggttt acaattatag taagaaaatt tcaggaggtt
    50761 tcattatggc acaacgttac caaaatatca tggtcgcaat cgatggttct aaggaagcgg
    50821 acttggcttt tgtcaaggga gttcattctg ctctacgaaa cgacgctaaa ctcaccatcg
    50881 cacatgtcat tgacacacgc gctctccaaa gcgtatccac ctttgatgct gaagtttacg
    50941 aagaactcca agtcgacgct gaaagtctga tgaaagagta cgaaaaacgt gctaaagatg
    51001 ctggggtagc agatgttcat atcgtcattg aaatgggaaa tccaaagacc ctgctagcac
    51061 gtactattcc agatgccgag gaagtggacc tcatcctcgt tggcgcaact ggtctcaacg
    51121 cctttgaacg cctcttggtc ggctcttcat ctgaatacat actccgccat gctaaggtcg
    51181 atttgctggt tgtaagagaa caagaaaaaa ccttataatc acaaagaaaa ggagccccta
    51241 gctccttttt gtttacgatt tatttctctc tttatggcgt tcgtaagcct tgagctggcg
    51301 ctgcagttcc tttttaatag caggttctgg agcatatttt tcttcccaat tatctggttt
    51361 taagatttta tgggtcactg gatcaaaatg agccttacca tctggaaaaa ttttccccat
    51421 attggcctga tggacaatat caaaaatacg ttctgggtcc acccccatca agacaaaact
    51481 gccgtaggtg aagtaaagca tgtcaatcaa ggcatccact tgccctatca aatcttgctg
    51541 agcaggtgtc ttcttggcta ctttatctgc tgccttatca agggcctgat gaagttgcga
    51601 tacagcttga ccaaaatctt cttcagaagg actggctgct cgaacaaact ccaccaattc
    51661 ttctatttta aaaccagccc tatgggttgc accctctaaa tcccaagctc gaggttcttc
    51721 ttgggttcgt tcatccatca tgtggtggaa ggtcttgacc ttattgaaat gatagtcacg
    51781 gctgacaaag actttttctg aagacaaaac accaaaatgt tccaaggcct tgtggatccc
    51841 atcttgttga ttgctagtgg taatgtgctt ggcagcttcc ttgacactgc tactaccatt
    51901 tcccatagcg accgacatac caacgccagc cagcatttca aggtcattat ctgagtcacc
    51961 aaaagccatg acttggttga ggtcaaagcc atattctttc ccaactcggc gaatgccttc
    52021 taatttagaa tttccctgat tgatgacatc cgatgcaaaa ggattgctac gtgtcaattt
    52081 caagtcttca aaatcagctg ccgccttctc agattcttct ggtgtcatca gcatcaaaac
    52141 ttggtagata ggctgattca tcaggtgaag caggtcttct tccttttggg gaacaatctt
    52201 gctgaccatg cgattaaaag actgactcac cgtccgagtt aaaacagagg gaacgaagcg
    52261 actaattcgt tgagaaaaag aacccagacc aaaggacatg attttagaac ccaacatggc
    52321 atccttggtc cctagggcaa tctccgtgcc ctctttttta gcatagctaa ttagatggcg
    52381 caaatgtaac ttagaaatag ggctcgtgaa caagactctg tctttactaa agatatactg
    52441 gccattatag gttaccgcaa aatccagatc caaatcgtcc atcaattcct taacaaaaaa
    52501 aggtcctcgc cctgtcgcta cgccaactag taccccttgt tctttgacaa tcttaatcgc
    52561 gtccttagtg gatttcaaaa cactcttgcg attgttgacc aaggttccat cgatatcaaa
    52621 aaaaacagct ttgacttcca tcctatccca atctcccctt ttgtgataca atgattatac
    52681 cacatttcag aaagagtgag taaatcatgc ctaagaaaat ccttgtttta catacgggtg
    52741 gaactatttc catgcaggcc gatgcttctg gcgctgttgt gacgagttca gataatccca
    52801 tgaaccatgt gtccaaccca cttgaaggaa tccaagtcca cgccttggac ttttttaacc
    52861 ttccaagtcc ccatatcaaa cccaaacata tgctggtcct ctaccagaaa attaaagagg
    52921 aagcagataa ctacgatgga gtggtgatca cacacggaac cgatacttta gaggaaacag
    52981 cctatttcct tgataccatg gaagttcccc atatgcctat cgttctaaca ggagccatgc
    53041 gtagctccaa tgagctcggt agtgatggtg tttataatta cctaagtgct ttacgagtgg
    53101 ccagtgatga cagggctgct gacaaaggag ttttggtcgt tatgaacgat gaaatccacg
    53161 ctgccaagta tgtcaccaaa acacatacga ctaatgtcaa caccttccag actccaacac
    53221 atggccccct cggtctcatc atgaaacagg aaatcctcta cttcaaaaca gctgaacctc
    53281 gtgttcgctt tgaccttgat cacatacaag gtttagtccc tatcatctcg gcttatgctg
    53341 gtatgacaga tgagctgatt gatatgctgg atttagaaca cttggacggt ttgattatcc
    53401 aagccttcgg agctggtaat attcccaaag aaacggctca aaaattagaa agccttctgc
    53461 aaaaaggaat cccagtcgct ctggtatcac gatgctttaa cggtattgcc gagcctgttt
    53521 atgcctacca gggtgggggc gtacagttgc aaaaagcagg cgttttcttt gttaaagaac
    53581 tcaacgccca aaaagcccgc ttgaaactcc tcatcgccct caatgccgga ctaacaggac
    53641 aggctttgaa agactatatg gaaggctaat actcttcgaa aatctctgca aaccacgtca
    53701 cgtcgcctta ccgtatgtat ggttactgac ttcgtcagtt ctatctacaa cctcaaaaac
    53761 atgttttgag ctgacttcgt cagttctatc tgcaacctca aagcagtgct ttgagctgac
    53821 ttcgtcagtt ctatctacaa cctcaaaaca gtgtgttgcg cagcctttaa ccagccgcct
    53881 agtccgctct atggtattca ttaagtcaac atctcttgtt taagagcaca aaatcaggaa
    53941 atcttctcga ttccctgatt ttttctattt acgttttcgt gttgagctac gttctgtcaa
    54001 accatgaggt aagagaactt cacgttcttc caactcttcc ttatgcataa tcttggtcaa
    54061 catacgcata ctaatggcac caaggtcata aagaggttgg gcaatcgttg tcaagtttgg
    54121 acgggtaaag cgtgagattt gtgaatcatc actagtaata atttcaaaat cttctggcac
    54181 agaaacaccc ttatcagcca aaccgttcaa gactcctgct gccaactcat cacctgtcac
    54241 aactgctgca gttgcatttg atgaaatcaa acgctctgct aaggcgtaac catcatcata
    54301 gctatattta gattcaaata ccaaaccctc actataagcg attcctgctt ttttcaaggt
    54361 ttccttgtag ccaactaaac gaaccttacc attgatgtca tccactagcg gaccgctaac
    54421 gaaagcaata cgctcatttt ctttagcaag gtaactcact gcatcaattg ttgcttgctt
    54481 atagtcaata ttgacacttg gcaactggtg ctcaacatcg acagttcctg cgagaacaat
    54541 cggagtacgt gaacgcgaaa attctgagcg aattttatct gtcaagtgat aacccatata
    54601 gataatgcca tctacctgct ttgaaaagag ggtattgaca acagaaactt ctttctcgtt
    54661 atcttcatcg ctattagcta ggacaatatt gtacttgtac atttctgcaa tatcatcaat
    54721 ccccttagcc aaactcgaaa aataaccatt ggtaatattt ggaatcacga caccgacagt
    54781 ggttgtcttt ttacttgcaa gaccacgcgc aactgcattt ggacgataat ccaagcgatc
    54841 aattacctct agcacttttt tacgggtatt ctcttttaca tttttattgc cattgaccac
    54901 acggctgacc gtcgccatgg aaacacctgc ttcacgagcg acatcataaa tggttactgt
    54961 atcatctgca ttcattcctt ttcctgtcct ttctatctca cacattcttt tacaagtaga
    55021 ggtactgatt gaagctctat atctacttac aaaagtgaag atgtgaaaat ttcgttttca
    55081 tatttctact tattccattc tatcactaat tgtaaacact ttcaagtgtt ttttgaagat
    55141 tgattgaaaa aatttcatag aaaacctagg tttagctcct tgctaccacc ttagactaaa
    55201 caaaaaggag gaaactaagc cctcctaaag ttatagtaaa atgaaataag aacaggataa
    55261 atcgatcagg acagtcaaat cgatttctaa caatgtttta gaagtagagg tgtactattc
    55321 tagtttcaat ctactatagg tattgttcca ttcactaccg tcaattttag cacatagtct
    55381 tcatgaaaat attatatcat cataaccaac cagattcttt tgcgatatta gctgcctctg
    55441 ttcgattacc agcatccaat tttgaaagaa tattggtgac atagtttcga atagttccgt
    55501 ttgagaggta aagctgatct gcgatttctt ggttggataa gccccgggcg atccccttta
    55561 agacagcaat ctcttgttct gtcaacggat tgggacgggt catcaccact tccatcaatt
    55621 caggcgaata ctccttgcgt ccttcgagga cggtgtgcaa ggtttgcatg aggtctgcaa
    55681 cgcttcgttc ctttaataca taagcatcca ctccagcctt gaccgcacgt tcaaaatacc
    55741 cagcacgctt gaaggtcgtc accacaacca cctttgtttc aagcctttct gatcgtatcc
    55801 actccaagac ttcaagacct gtcttaacag gcatttctac gtcaaggatg gcgatatcta
    55861 cagactcctt ttctaatagt tggattgctt cttgcccatt cttggcttga aggacagact
    55921 ccacatctgg ttgaagcgtg agcaattgac acatggcatc tcgcaacata ctttgatctt
    55981 ctgcgactaa tactttcatc ttagtttctc tccttataag gtagtcgaac ttgcacttcc
    56041 gttggatgtt tctgactgat tacacttact tctcctgaaa atggaaaaac acgatctcgg
    56101 attgtatgga gctcatctcc ttttagaaaa gcaaagccgc agccatcatc acttactgtt
    56161 aaaatgagtt ccttctctgt ccgttctaat tttaagtaag ctttagacgc tttggcatgt
    56221 ttgatgatat tggtcaccaa ctcaagcaaa atcatagaag ccattgactc caattcttga
    56281 gtaaggctag cagtatctag ttggttatcc gtttccacct caattccagc aatttctaac
    56341 atctttttca cagtctctag ttcggatgtc aaagttctag acttaagatt ttccacaatg
    56401 gttcgaactt cacgcattga ttctttgcta atttgctgta tttctcttaa ttccttttcc
    56461 acttgtggat aagcctgcat ttgaaatagc tgcaaggcta gatctgtctt gacactaagc
    56521 atagcaaagg tatgtcccag actatcatgc aaatcctgac cgatacgatt gcgctcattt
    56581 tctgctagca atagatttat ctgggtattt tgcttgacct gagcttcttt caaatcctcg
    56641 acaaggcgaa ttcgaaccga gcccaaagtc atcaaatcga caaaagtaag aattacaagc
    56701 atatagacta gaaactcaac ttcgattctc tgaaaaatca aaagtcgccc cacaacaagg
    56761 acttgagcaa ggagaaatgt ccatacatgt aaagacttta aactacgtac gccaaaatga
    56821 taaattaaga gattggaaag gaaaaagaaa aaccagatat aattcccagc aacaaaggcg
    56881 gtattcccag ctacatagac cagcatgatc ccccagaaaa gccatgatat gcgctggttc
    56941 ttagttatta aaacacccaa atacgtcact acaaatagaa tatcaaccaa taaatgccaa
    57001 gcaggaagct ccccagtcac tacaggtagg atggggaaaa tcataaaaat taaacttgcc
    57061 caaaacatat agtgtatgct tttcagtctt tcaagcatta agcattctcc ttatgacctt
    57121 gaaggtaaat ggtcaaacca aacaaaactg ctgaaaaaac aagtagataa actgtggctg
    57181 atagattgat gccaccctcg tttaagaagg tcttgagcaa ctccatcaat tgataggtcg
    57241 ggagacactt accaactgct tgcatccagt ctggaaataa agaaatgggc atccagagtc
    57301 cgcctaaaac agccaatcct agataaagaa gattgcccac aacagacatc agctgactag
    57361 ttggtaagag agtcaaggtc aagccaagtg ctacaaaagc aatactcccc acaatcagca
    57421 aaatcgcagc tccaacccat tttactagag gcatgtctac acctcttaca aaatgtccaa
    57481 ctgtaaaaac aaccagaatt gagaccaaat aatcaaccag catacttgtt atctttgata
    57541 gataatattc taccatattt acagggctat gacgcaatgt tttctgccag ttgttcatct
    57601 tatcggtatg taaaacagct ggaaatgaga ggatagttgt cgacatcatg gaaaaggcag
    57661 tcatggagat gaggtaatca cgcataaaat tcgcgagttc acctggtgtg tcctgataga
    57721 taccagaaaa aaataaatag aaagccgtcg gcatccctac cgacaataga taatagatta
    57781 attgtcgttt ggtcaataaa aattctatct tactaagtgc tatccatcgt ttcatcttag
    57841 ttatctccct tctgcgtctc ttcaaagatt gtattcaaca aactacgatt attaacttcg
    57901 atttcttgca tcctacatcc tgcttgggct aatagttccc agaaagcatc tgcttctcga
    57961 gtgactactt gtaaagaatc ttgctttagg gtccagtttt caaccaaatt tgactgctct
    58021 acgacttcct tgtaagctat aggaaggata aagtgctttt ctatttcctc gctacgcata
    58081 gctagaggcg tcgtatctcg aatcaactct cccttattta agagcaaaat gcggtctgca
    58141 gtgtgttcca cttcctcaat ataatgggac gaatagagaa tggtgactcc ttgcgctttt
    58201 aactcctgga caatttccca aaaacgttga cgagttgagg tatccatgga cgcagtaggc
    58261 tcatctaaaa aaacaatctt tggtcgccca attaaggtca agacgaaaga aaagagacgc
    58321 ttttgaccgc ctgataattt ttctgccaac tgttcttttt gttgcttgac aaactgcaat
    58381 agttgatcaa tttcctgatt gctcaaagga tttggataga tacgttgaaa gaaagcaatc
    58441 aactctttaa cttttaattt ctgaacgatt acattttctt gagggaggta agccctagta
    58501 tagtctaact tagaactcgt cactggtaaa ccttgaatag atacttgacc gcttgtgacc
    58561 agtttatctc caagcaaaca atctaagagt gttgtctttc cggcaccatt tggaccaatt
    58621 aaggcaacac attcaccatc gttgatttca aaggagatat tccgcaaaat ttccttgtct
    58681 tttattttct tactcaattt ctcaacttta accaccgtca tgagactctc ctttcaacca
    58741 ctccattccc ataaggaaaa cgacgaaaat cataaatcca aaccccaaag caccacgaat
    58801 gaattggcga agcaaggctt gatcaaacca atcagtaaac atttccacta accataccaa
    58861 gagtgacagg ccgataaaga aatagatgat ccctctcttc attcctcaaa ctcctttttc
    58921 acatctctga ctaatttcaa accttctcta acaagccaag acatgattcc aaagccagca
    58981 aagaccttcc aaggaaaatg gtagaaatcc tcatccaatc ccgaaaacat gagataggtc
    59041 ataactcctg ctactactaa acttactgcg ataatcattt tatttctcat ctcttcttcc
    59101 cccatttcat actacaatta tagtctttta aaatcagagg agacagaagc ttctgtcact
    59161 aaaaaatatg acaaatgtca taaaaaattc tgttcaaaac aagcaagata cactatacaa
    59221 taaaacacaa tcagaaaaat ctaaggcaac ttcctcaaaa gagatatcaa acccaattca
    59281 caccataatg taaactaata cttatttaaa atcaaaaaga gtaaaaattt ttatcagaca
    59341 aacacatata tagtgtattg aatctataac agtaggcctt aaatactaaa atatttctat
    59401 aaattaattt aactttcctg atagaactgt tcatatctta tttcaattct ctatattata
    59461 cgttgaacca aacccttcta ttgctttctt aaaaatttat aagagttata aaatctgtta
    59521 aatttcaatg tgtatatcta aactacggta tttattgaaa agactggaga caaaaagtat
    59581 acgctgccaa aatgaattac tgaaaatcaa aaaagagaga accaaactga ttccctctta
    59641 atgtatataa tatctagttt taaaaataca cactcacata tctctgt
//
LOCUS       DBEROU010000007        54399 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.007, whole genome shotgun sequence.
ACCESSION   DBEROU010000007 DBEROU010000000
VERSION     DBEROU010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 54399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54399
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.007"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            264..1643
                     /gene="bglA"
                     /locus_tag="ACL8CL_000471"
     CDS             264..1643
                     /gene="bglA"
                     /locus_tag="ACL8CL_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGS1329775.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNPQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KELSDNNGF"
     gene            1835..3643
                     /gene="glmS"
                     /locus_tag="ACL8CL_000472"
     CDS             1835..3643
                     /gene="glmS"
                     /locus_tag="ACL8CL_000472"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HGS1329776.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEKYLAGHHFKGQTDTEIAVHLIGKFAEEDGLSVLEAFKRALHIIRGSYAFAL
                     IDSENPDVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQAFDLVHELSIVAQSIESTLSEKET
                     IEAKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(3720..3857)
                     /locus_tag="ACL8CL_000473"
     CDS             complement(3720..3857)
                     /locus_tag="ACL8CL_000473"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329777.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEVVDRTDEISNHTYGKATLTWFEEI
                     F"
     gene            3914..4963
                     /locus_tag="ACL8CL_000474"
     CDS             3914..4963
                     /locus_tag="ACL8CL_000474"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HGS1329778.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVGLD
                     VYGIGEHHRADFAVSAPEIVLTAGAVNTKKIRLTSAVSILSSMDPIRLFQQYVTIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGPNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(5063..8860)
                     /gene="spuA"
                     /locus_tag="ACL8CL_000475"
     CDS             complement(5063..8860)
                     /gene="spuA"
                     /locus_tag="ACL8CL_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HGS1329779.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKSEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHIQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASHPAHQDPAPEARPDSTKPDAKVADAENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            9276..9689
                     /gene="rpsL"
                     /locus_tag="ACL8CL_000476"
     CDS             9276..9689
                     /gene="rpsL"
                     /locus_tag="ACL8CL_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HGS1329780.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            9709..10179
                     /gene="rpsG"
                     /locus_tag="ACL8CL_000477"
     CDS             9709..10179
                     /gene="rpsG"
                     /locus_tag="ACL8CL_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HGS1329781.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            10604..12685
                     /gene="fusA"
                     /locus_tag="ACL8CL_000478"
     CDS             10604..12685
                     /gene="fusA"
                     /locus_tag="ACL8CL_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HGS1329782.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            12789..17180
                     /locus_tag="ACL8CL_000479"
     CDS             12789..17180
                     /locus_tag="ACL8CL_000479"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HGS1329783.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQAANEEALRAMEQLEQMTPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLARLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVSRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNEIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNDYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEVIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            17282..17545
                     /locus_tag="ACL8CL_000480"
     CDS             17282..17545
                     /locus_tag="ACL8CL_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HGS1329784.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQVLTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            17538..17816
                     /locus_tag="ACL8CL_000481"
     CDS             17538..17816
                     /locus_tag="ACL8CL_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
                     /protein_id="HGS1329785.1"
                     /translation="MLKIRYHKQFKKDFKLAMKRGLKAELLEEVLNFLVQEKEHPARY
                     RDHSLTASKHFQGVRECHTQPDWLLVYKVDKSELILNLLRTGSHSDLF"
     gene            17837..17989
                     /locus_tag="ACL8CL_000482"
     CDS             17837..17989
                     /locus_tag="ACL8CL_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329786.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALREK"
     gene            18011..19252
                     /locus_tag="ACL8CL_000483"
     CDS             18011..19252
                     /locus_tag="ACL8CL_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HGS1329787.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVGGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            19262..19492
                     /locus_tag="ACL8CL_000484"
     CDS             19262..19492
                     /locus_tag="ACL8CL_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGS1329788.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            19715..20437
                     /locus_tag="ACL8CL_000485"
     CDS             19715..20437
                     /locus_tag="ACL8CL_000485"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS1329789.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
     gene            20654..21988
                     /gene="pepC"
                     /locus_tag="ACL8CL_000486"
     CDS             20654..21988
                     /gene="pepC"
                     /locus_tag="ACL8CL_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HGS1329790.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALERRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(22044..22955)
                     /locus_tag="ACL8CL_000487"
     CDS             complement(22044..22955)
                     /locus_tag="ACL8CL_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1329791.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(22979..23782)
                     /locus_tag="ACL8CL_000488"
     CDS             complement(22979..23782)
                     /locus_tag="ACL8CL_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HGS1329792.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(23810..24799)
                     /locus_tag="ACL8CL_000489"
     CDS             complement(23810..24799)
                     /locus_tag="ACL8CL_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1329793.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
     gene            complement(25032..26051)
                     /gene="adhP"
                     /locus_tag="ACL8CL_000490"
     CDS             complement(25032..26051)
                     /gene="adhP"
                     /locus_tag="ACL8CL_000490"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HGS1329794.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSIRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVVPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
     gene            complement(26390..27202)
                     /locus_tag="ACL8CL_000491"
     CDS             complement(26390..27202)
                     /locus_tag="ACL8CL_000491"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1329795.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKRTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKEMNAFYQHQLSINTWGGPLNILECTPKGVNKSFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            27392..28810
                     /locus_tag="ACL8CL_000492"
     CDS             27392..28810
                     /locus_tag="ACL8CL_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HGS1329796.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFAMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            28863..29570
                     /locus_tag="ACL8CL_000493"
     CDS             28863..29570
                     /locus_tag="ACL8CL_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1329797.1"
                     /translation="MKEKNMWKELLNRAGWIMVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVAGLSIVVLALFIIGARKTQLASFNFSFFRAKDLARLGLSYLVIVGSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            29665..30639
                     /gene="folP"
                     /locus_tag="ACL8CL_000494"
     CDS             29665..30639
                     /gene="folP"
                     /locus_tag="ACL8CL_000494"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HGS1329798.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAEARAQVVIMFNPVMARPQHPSSLI
                     FPHFGFNQTFTVEELADFEILPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRNLDKLHQKGYPIFLGVSRKRFVINILEENGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            30641..31963
                     /locus_tag="ACL8CL_000495"
     CDS             30641..31963
                     /locus_tag="ACL8CL_000495"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HGS1329799.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFNSPYLIHYTDQISINGESISEARLEALMADY
                     QSLMGGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLEAIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEGKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGRFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADY
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKSRNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIVTGSLYFLSQVRAYLMERKNENGYTKD"
     gene            31944..32498
                     /gene="folE"
                     /locus_tag="ACL8CL_000496"
     CDS             31944..32498
                     /gene="folE"
                     /locus_tag="ACL8CL_000496"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HGS1329800.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            32541..33353
                     /gene="folB"
                     /locus_tag="ACL8CL_000497"
     CDS             32541..33353
                     /gene="folB"
                     /locus_tag="ACL8CL_000497"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HGS1329801.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIEKMRARGIYILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(33398..33715)
                     /locus_tag="ACL8CL_000498"
     CDS             complement(33398..33715)
                     /locus_tag="ACL8CL_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HGS1329802.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            34072..34518
                     /gene="rplM"
                     /locus_tag="ACL8CL_000499"
     CDS             34072..34518
                     /gene="rplM"
                     /locus_tag="ACL8CL_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HGS1329803.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVVNAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            34537..34929
                     /gene="rpsI"
                     /locus_tag="ACL8CL_000500"
     CDS             34537..34929
                     /gene="rpsI"
                     /locus_tag="ACL8CL_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HGS1329804.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            35278..35487
                     /locus_tag="ACL8CL_000501"
                     /pseudo
     CDS             35278..35487
                     /locus_tag="ACL8CL_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            35643..35981
                     /locus_tag="ACL8CL_000502"
     CDS             35643..35981
                     /locus_tag="ACL8CL_000502"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HGS1329805.1"
                     /translation="MTLTIHVYYKGDGDSAIKFAREMLDSGLVEEIRNQKGNLKYEYF
                     LPIEKEGTILLIDQWINQEALDEHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPE
                     SDKSFIDTGN"
     gene            36171..37607
                     /locus_tag="ACL8CL_000503"
     CDS             36171..37607
                     /locus_tag="ACL8CL_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HGS1329806.1"
                     /translation="MTTFKDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITLGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSLAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYQEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPVTCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTDQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWKWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMIHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            37659..37898
                     /locus_tag="ACL8CL_000504"
     CDS             37659..37898
                     /locus_tag="ACL8CL_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HGS1329807.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            38028..38342
                     /locus_tag="ACL8CL_000505"
     CDS             38028..38342
                     /locus_tag="ACL8CL_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HGS1329808.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
     gene            38460..40439
                     /locus_tag="ACL8CL_000506"
     CDS             38460..40439
                     /locus_tag="ACL8CL_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1329809.1"
                     /translation="MNVLNIKEKDILQFLIKNKERFVTSKELAEYLSCSDRTVRNVLK
                     LIEKTMIIQGVRLISKQGQGYQIFFENQGAYQEFRQTYELEEDYTKTAVSKGDDRLVF
                     ILNKLLFEQVPVLFDDLADELYVSRSTLSHDFRKIRVMLSEYNLSIESRANKGVYVSG
                     EERDKRRFIINYFLENQFFKTIHCYVKFNFFDQTVPLEEFARIVLDECQEANLKLSDF
                     VLQNLVVHIALSVIRLKSGFEIKNIDCQMTDDAIERKVAQRILSKVREVTNQEFPVQE
                     IDYITLHLLAKSQQCQKNQKNISEEVLKKSLLKTFQNLGLDDMYNFSSDFQLIEGLIT
                     HLMTLQVRLESRITLNNPLVDEIKQNYSDIFFMTREILANMDKFLEWTISDDEVAYVS
                     LHFLAAMERSKESTKFNILAICATGFGAAQMLRNRLETEFGKRVEVVDVIGYYELNQE
                     KLKGIDFIVSAVDLSNLYFQIPVFKVSVFLKSDEMEMIRKAMDQMQVSSYVQSSKINK
                     FENNGFRQYFSKENFLICTESDKVNLLEKMVESLSVGESNEFEQSLLYGIKQREELSS
                     VVFSEKIAVPHPIQSFGTEGKVSVAICKDSLLWDNQSSHVQLVFLLSPSIYGNEGLAT
                     VTKKIVSLTENDELQNQLISCNNFEDFINIFEKIK"
     gene            40449..40763
                     /locus_tag="ACL8CL_000507"
     CDS             40449..40763
                     /locus_tag="ACL8CL_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HGS1329810.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            40794..41300
                     /locus_tag="ACL8CL_000508"
     CDS             40794..41300
                     /locus_tag="ACL8CL_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329811.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LSQFISPSPIIIFPIMLIVFSILATVEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLSLLDIRRIKKIYKRTDKAYQQFVQ
                     LEKNSLSL"
     gene            41380..42726
                     /gene="celB"
                     /locus_tag="ACL8CL_000509"
     CDS             41380..42726
                     /gene="celB"
                     /locus_tag="ACL8CL_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HGS1329812.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITAAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            43105..43281
                     /locus_tag="ACL8CL_000510"
     CDS             43105..43281
                     /locus_tag="ACL8CL_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329813.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALALGMT"
     gene            43683..45896
                     /locus_tag="ACL8CL_000511"
     CDS             43683..45896
                     /locus_tag="ACL8CL_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HGS1329814.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSQTGSFEDRTTQLIYNRDFGQ
                     ISLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYGSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVQTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(46118..46594)
                     /locus_tag="ACL8CL_000512"
     CDS             complement(46118..46594)
                     /locus_tag="ACL8CL_000512"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HGS1329815.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLQDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            46817..50017
                     /locus_tag="ACL8CL_000513"
     CDS             46817..50017
                     /locus_tag="ACL8CL_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HGS1329816.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTIGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSPWTGSKAQGWSAWVDQKNSADASTRVIEAKDGA
                     ITISSHEKLRAALHRMVPIEAKKKYKLRFKIKTDNKIGIAKVRIIEESGKDKRLWNSA
                     TTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVADQLSEDSQTD
                     KQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKEV
                     KKIPLKILASVKDAYTARLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSIS
                     SQADRTYLWEKFSNYKMSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSMEW
                     LHKYVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPE
                     HFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVEQGEGFY
                     QDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKSPIDKDKMQTMYHWIDKSFAPL
                     LVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQS
                     DSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGFG
                     LSLFSSRTLNYEHMNKENKHGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTET
                     DAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFLG
                     SNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDSK
                     KNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYML
                     IPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVLK
                     RGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKNH
                     SDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            50397..50773
                     /gene="tnpA"
                     /locus_tag="ACL8CL_000514"
                     /pseudo
     CDS             50397..50773
                     /gene="tnpA"
                     /locus_tag="ACL8CL_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            50955..51017
                     /locus_tag="ACL8CL_000515"
                     /pseudo
     CDS             50955..51017
                     /locus_tag="ACL8CL_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596946.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(51161..51790)
                     /locus_tag="ACL8CL_000516"
     CDS             complement(51161..51790)
                     /locus_tag="ACL8CL_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HGS1329817.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYVTLR"
     gene            complement(51800..52801)
                     /locus_tag="ACL8CL_000517"
     CDS             complement(51800..52801)
                     /locus_tag="ACL8CL_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HGS1329818.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITIAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKEAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(52832..53473)
                     /locus_tag="ACL8CL_000518"
     CDS             complement(52832..53473)
                     /locus_tag="ACL8CL_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HGS1329819.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKDYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKTIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(53492..54307)
                     /locus_tag="ACL8CL_000519"
     CDS             complement(53492..54307)
                     /locus_tag="ACL8CL_000519"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HGS1329820.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIEAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNPEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
BASE COUNT        16232 a         9881 c        12096 g        16190 t
ORIGIN      
        1 atccctattg acgacatcta taatttggat atttgtgtct ttgatatcga gtagttttgt
       61 gataaaatgt aattgttcca tatgaatctt tctaatgagt tgtttggtcg cttttcatta
      121 tagatcttat gggacttttt ttctacaata aaataggctc cataatatct atagtggatt
      181 tacccactac aaatattata gagccgtttt taccttgttt tttctgtata atgggagaaa
      241 tgagtagata aagaggtaaa tgtatgctaa gatttccaaa ggattttgtc tggggatcct
      301 ctacttctgg accgcaaaca gaaggacgtg tagctggtga cggtaaggga gacaatctct
      361 gggattactg gttccaagtg gagccaaatc gttactataa tgggattggt tcagataaga
      421 catcgacttt ttatgaaaat tgggagcggg atattgagct tttgttagag actggtcaca
      481 cagcctttcg gacttctatt cagtggtcac ggatttttcc acaaggctgt ggaaaagtca
      541 accctcaagg tgtggatttt tatcgtaagg tctttgaggc tattaaggct aaaggaattc
      601 gtctgttagt caatctctat cattttgatt taccttttgc ccttcaagag gatggtgatg
      661 gttgggaaaa taaggcgaca gtctcagcct atgaagacta tgctcgtttt tgttttgaga
      721 cttatggaga tttagtggat caatggatta cctttaacga gcccatcgtt cctgtagaat
      781 ttggttattt ttatgatgcc cattatccac ataaggtgga tgcagaggca gctgttaagg
      841 tagcctatca tacacaattg gccagcagcc gggctgttaa ggcttgccat gaacttttgc
      901 ctgattccaa gattgggatt gtcctcaact tgacaccggc ttatccacgt agccagcatc
      961 ctgctgatgt caaggcagct cgtattgcgg ccctttttca ggcccaatct ttcttagatc
     1021 catctgtctt gggaacttat ccacaggagt tagtagaaat cttgcatgaa cacggtcttt
     1081 tacctgatgc tacagaggaa gagttggaac tcattcgtga taatacggtg gacttccttg
     1141 gtgtgaacta ctatcaacct ttgcgtgtta tggctcctcg atttgctaag catccagaga
     1201 gtccactctt accagaacat ttttacgagc cttatgtgat gcctggacgt aaaatcaatt
     1261 ctcaccgtgg ttgggagatt tatgagcaag ggatttatga catcgcccaa aatatcaagg
     1321 aaaattatgg caatattgag tggatgttga ctgagaatgg tatgggtgtt gaaggggaag
     1381 aaaaattccg tcaagatgga atgattcaag atgattaccg tattgacttt gtaaaaggtc
     1441 atcttcgtga acttcaccgt gccattgaag atggtgccaa ctgtaagggc tacttgattt
     1501 ggacctttat tgactgctgg tcatggctca acagctataa aaatcgctat ggtttggtcg
     1561 aattagactt ggaaacgcaa gaacgtcgtc tgaagaaatc aggccactgg ttcaaagaat
     1621 taagcgataa taatggattt taaaaaggac tctgactgat tatcccaatc ggtcagagtt
     1681 ttttgcttac aggagcttaa tttgaactat accaattttt actcttgaca agtgaaagaa
     1741 acaagtatat actgtttttg ctgattctat aaaagatgaa ttagactata ccaattttaa
     1801 ggagaaagca cagcttgcct gtgtcgtata tactatgtgt ggaattgttg gtgttgttgg
     1861 aaacacaaat gcaactgata ttttgattca agggcttgaa aagcttgaat accgtggcta
     1921 tgattctgcg ggaatttttg tcctagatgg tgctgataac catttggtga aggctgttgg
     1981 tcgtatcgca gaattgtctg ccaagacagc tggtgttgag ggaacaactg gtatcggaca
     2041 tactcgttgg gcaactcatg ggaaaccaac ggaagacaat gctcacccac accgatctga
     2101 gacagaacgt tttgtcttgg ttcacaatgg ggtaattgaa aactaccttg aaattaaaga
     2161 aaaatacctt gctgggcacc acttcaaagg gcaaacagat acggaaatcg ccgtacattt
     2221 gattggaaaa tttgcggaag aagacggtct ctcagttctt gaagccttta aaagagctct
     2281 tcatattatc cgtggttcat atgcctttgc cttgattgac tctgaaaatc cagatgtcat
     2341 ctatgtagcg aaaaacaaat ctccactttt gattggtctt ggggaaggct acaatatggt
     2401 ctgctcagac gccatggcta tgattcgtga aactaaccaa tacatggaaa tccatgacca
     2461 agagttggta atcgtcaagg ctgatagtgt ggaagttcaa gactatgatg gaaatcgtcg
     2521 tgagcgtgct agctacactg ctgaacttga cttgtcagat atcggtaagg gaacttaccc
     2581 ttactacatg ctcaaggaaa ttgacgagca accaactgtt atgcggaaac tcatccaagc
     2641 ctacacagat gaggctggtc aagttgtcgt agacccagat atcattaagg ctgttcaaga
     2701 cgcagaccgc atctacatcc ttgcagctgg aacatcttac catgcaggat ttgcttctaa
     2761 gaaaatgttg gaagaattga cagatacacc agttgaactt ggaatctcat ctgagtgggg
     2821 ctacggtatg ccacttctca gcaagaaacc actcttcatc tttatcagcc aatctggtga
     2881 aacagcggat agtcgtcaag ttttggtcaa ggctaatgaa atgggaattc caagcttaac
     2941 agtgacaaac gttccaggtt caaccctctc acgtgaagcc aactatacca tgctccttca
     3001 cgcaggtcct gaaattgccg tggcatcaac taaagcctat acagcgcaaa tcgcagccct
     3061 tgccttcctt gcaaaagcag tcggagaagc aaatggtaat gctaaagcgc aagcctttga
     3121 cctggttcat gaattgtcaa tcgtagctca gtctatcgaa tcaactcttt cagagaaaga
     3181 aaccattgaa gccaaggttc gtgaacttct tgaaacaact cgtaacgcct tttacatcgg
     3241 acgtggtcaa gattactacg tagccatgga agcaagtctc aaactcaaag agatttctta
     3301 tatccagtgt gaaggttttg cggcaggaga actcaagcac ggaaccattg ccttgattga
     3361 agaaggaacg cctgttttgg ctctcttgtc agatccagtt cttgccaacc atactcgtgg
     3421 aaatatccaa gaggtcgcag cccgtggtgc caaagtcctc actatcgcag aagagaatgt
     3481 agccaaagat accgacgata tcgtccttac gaccgtacat ccatacctct caccaatttc
     3541 aatggtcgta ccaacgcaat tagtcgctta ctttgcaacc ctccaccgtg gcctcgatgt
     3601 ggataaacca cgtaaccttg ctaaatcagt aacggtagaa taagctaaaa aagtctagtt
     3661 atctaggctt tttctaatga gcaaattggt tgcttgtact taagattcgt gttatactct
     3721 tagaaaatct cttcaaacca cgtcaacgtc gccttgccgt aggtatggtt actgatttcg
     3781 tcagttctat ccacaacctc aaaacactgt tttgagcaac ctgcggctag cttcctagtt
     3841 tgctctttga ttttcattga gtattagaat aatttttcaa attgaaggac agagatagac
     3901 aaggagaatc acaatggtag aattgggaat ttcaacattt ggggaaataa cggagcttga
     3961 agggactgga caaacttaca gtcatgctga acgcattcgc cagttggtgg cagagattga
     4021 gctggctgac aaggttggtt tggacgtata tgggattggc gagcaccatc gggcggattt
     4081 tgcagtatca gccccagaga ttgtcctgac cgctggggca gtcaatacca agaaaatccg
     4141 tttgaccagt gcagtcagca ttctgtcaag catggacccg attcgtttgt tccaacagta
     4201 tgtcactatc gatgctttgt caaatggacg agcggagatt atggctggaa ggggctcttt
     4261 cacggaatct ttccctctgt ttggttatga cttgaaagac tacgactctc tttttgatga
     4321 gaaattagac ttgcttcagt tagtcaatga aaagaccaag ttagactggc aaggtcgatt
     4381 gactcaaacg attgctggta aagaagttta tcctcgtcca gttcaggaca aattgccctt
     4441 gtggatagct acaggtggtc atgtcgaatc aacagtgaag attgctcagg ctggtctacc
     4501 gattgtatat gctattattg gtggtaatcc acgttatttt aaaaagttga ttcaggctta
     4561 tcgtgagatt ggaagcgaag cgggccatgc tgacaaagac ttgaaagtgg gagcccattc
     4621 ttgggggtgg attgcggaag atggtgagca ggcggtgaaa gattatttcc atccgactaa
     4681 gcaagtggtg gatgctattt ccaaagaccg tccgcactgg caggaattgc gttatgagca
     4741 atatttggag caggtagggc caaatggcgc catgtttgtt ggcaatccag atcaggtggc
     4801 cgaaaaattg attcgcatga tagaggattt aggattggac cgcttcatgc ttcatctacc
     4861 gcttggttct atgcctcatg accaggttct gagagctatt gaactcttcg gaacacaagt
     4921 tgcacccaaa gtacgagctt attttgctat gaaagaggct taataaaaaa tcctaatcaa
     4981 gtttattagg acaacaaata ttgtacaaaa attaatcccc cgagctataa tgcttggggg
     5041 atttttctat aggagggcta gtttagttct cttttttatt ttttagtaag aaaccgagac
     5101 caaggagcgc aaggaggctg attcctgcaa ataggagttt gttttcgttt ttgattcctg
     5161 tatttggaag ttcagcttgt ttatctgggg ttgcaggtat attttccttg ctagagtttt
     5221 ctaccgattc gtttcgaacc gcttctttta cagatgatgc ttgtgcttct tgtgctggtt
     5281 gttcagcttg tgaatcagct gtagcttggc taggtttatt ttccgcatca gctactttgg
     5341 catctggttt agtagaatca ggtctagctt ctggagctgg gtcttgatgt gcagggtgag
     5401 aagcttcatt ttgatgttca ggcttggaag gggttgagtc tggtttctct tctgcagttg
     5461 actcatggct agttccattt tgagagactc gaagaacagt agctgtaagg gcattcaatt
     5521 tcaagccttt ttcagtccat tcaagtcctt tcgggttggc aattccgact gatcctgctt
     5581 ggttttcatc tgccaaaact tccgcatttc ttagatgtgc aaaggcagtt cccaaattaa
     5641 attcgcgagc tttttcatcc gcattgacaa agactgcgta gatatcgccg tttggagcag
     5701 tgatttggta gccaatcact acatcctctt tttccacacc attttggcct gggacagtga
     5761 tgaggtggac acggtctttg atatcttgga gactcttaag tcggaaggca tctgtagatt
     5821 gacgaagggc aatcaaacct ttcatatagt cacggctctt gacattttca ggataagctt
     5881 taccatctgt agccttagtc cagtcaaact tgttgactgc gtcgctagaa tcgtaagaat
     5941 catggatgaa gtaaggatag tcaaatgggt tgccgtcctt atcacgcaac aagtgagatt
     6001 tgtttggaac cttatcctct gctactggag tcttgtaggc tgggtcacgg aattgtttag
     6061 tacgtccata ttcctgaccg gagtggataa atggagttcc ttgagctgtc aagaccatga
     6121 gatttccaag tcgtaaacga cggtggattt cagcatagtt ctcagccttg cttgggtctt
     6181 ttttgataga ctgggcaatg atgtcaaaga gggtcaagtt atcatgggct gcgatgtatt
     6241 ggatgacatc tccagggctg tcagcttcaa agttagttgg ttgagcaatg agatttttaa
     6301 agatggtgtt gacatcacgc ttgccacctg tgataaaggc aggttgacct tcgtttggat
     6361 aaccagattt gaggttgtta cggatgtcat ctgaaaagac agcgacagta tcggtatgtt
     6421 tcatccaatc ttggtcagca gctttagtag gcatgttttc atcaccggca taggttctcc
     6481 aaccttcacc aagcatgatg aggtttggat tgagggcgcg tgcagccttg taagcttctt
     6541 cgatagaagc ggcgtcatgg tctcccatca tatcgaagcg gaagccatcc actttgtagg
     6601 tatcaactag gtatttgata gagtcaacta ggagccgttt ggtcatatgg tgggttgtcc
     6661 ccaagcgtcc accaccaaag ctagttcgag gtgtgccatc ggcatccata aagtggtagt
     6721 agtttggctc taggtcttca aagatatcga ctttggctgt gtggttataa acgacatcta
     6781 ggatagctcc cataccacgt ttgtggattt cgttgatgag gtttttaaat tctgcgattc
     6841 gtttttctgg attcttagga tcgcttgagt acataccagt caaggagaag tagttttgag
     6901 ggtcatatcc ccagttgtag ttgctgttgc ttgaagcgta gtcagacaag cgttcatggt
     6961 ttttcaattc attgacaaag tagtaagaca agactggaag gagctggata tgggttacac
     7021 ccaagtcttt gagatagtct agtttttcaa tgaaggcttc aaaagtccca aatggtttgg
     7081 tcaagtcttt tgcaatggca ggatctgaag tgaagtcacg cacatgagct tcgtagataa
     7141 cggcgtcttc acgagtcttg aaattgtgaa tcttaccata agtcaagtct tgaggtccga
     7201 gtttagctgg atctacaaag gcggctttag ccactttatg ggcatcgtca attttggcat
     7261 cgtcgctatt ccaagcagca agagatttag cgtaaggatc gagtgcaaga acagttttac
     7321 cttgacgctc gatttggtat tgataatagt agccagtgaa atctgtgatt ccgagtttgt
     7381 ttgtgctgtc tagagtttgt ttccaagttc ctctttcccc tttttcgaga gcgacagttc
     7441 caactacttt gtcagggtca ttcttgtcgt agacaacaac agaaacctta tcagcacttg
     7501 gtgaccaaag ggtcaaatca acttgttttc cttcttcttt taggtcagct cccagtttgc
     7561 catcatagct gtatgtctca tctttcaggc gccagcttgt tttggtagtg aattggtcgg
     7621 aattgtagct aacagtataa ggatgttttg tgtcagagaa atctccgctg taggtcactt
     7681 tcttaccagc ttcatcgatt gcaacatcgg taatagttac cttgtttcct aggtgattag
     7741 tgatgttgga gtgtttgagg atatcttctt ttttagcacc gacaagtgtt gaaaagctac
     7801 tttcaatgct agaagtgcct acgtgttggg ctcctgtcat acggatatca tggacatagt
     7861 atggatttgt gtaaatcgat tcatcatcgt cttttaggaa aatttggcta tgatttttca
     7921 aatctgtgaa cttataattt tctttacgga ttttcacgtc gtctccttgt ttgctctcat
     7981 ctagtaataa aaatccaaat tctcttgcgg cttcattaag aggaatgtcg atatagcggc
     8041 catatttgcc tgtagccgta aagtctgttc cgtcaggcca ttgagcgcta cttggatttt
     8101 ttacatctcc ccagtaccag agagatttct tgtcatagtt gccatctgtg cggtagtagt
     8161 tgacgcgaac agttcctgca ggctgtggct cgtaagagaa aaccttgtaa tcttggtcta
     8221 accaagcctc gttcattttt ggaactagtt tttctacaga tttatcgccg gttagatttt
     8281 ttccagctgt attgttgatg aggaagctaa ttttcttggc ttgttctccc tttaatttga
     8341 catctaggta atagccgtag tcatctttct tggcatcctt gaaggacaaa gctccgtttg
     8401 gccagttttc agatggtttt tcaacatcgt cccaagtcca tagtccttga gcatccttgt
     8461 tttcttcagg aagttttttg acatggatac ggaagtagtt atcttcgatg ggctcttctg
     8521 acttagtttc agttgttact ggactagtag aagtagttgg ctctgttgca ggtttttctt
     8581 ctgttttctc tagggaagcg tttgcatttc taggtgaaga aatatcactt tcgttcttac
     8641 cagagccagc tgctgtatca gtaagaggtt gggcaagggt ggttgtattt ctgttgttgg
     8701 gttcgttagt ggttggtgta ctttcgtcgg atgagatagt tggtgtagcc atggcaagca
     8761 agacaaggct tgcgccgata aggacagaac cagttccatt tttgagggaa cggatgctgt
     8821 agaccatttt tttctcagtg tgagatggtg tttttctcat aatgattctc caatcaataa
     8881 atttctatat cagtatagca tgtagtaaaa caatatgcaa gcgttttctt tgatttgtga
     8941 aaaaattctt acttttgaga agttttctgt actattttat atatcagtat aaatgacgga
     9001 gataaaaatg tcacataagc caagccgtat tattaggaag atgatgtgtt cccagctatt
     9061 tttattcaga aattgatctc taacattacc aataaaaaag aaaaatatct tgacaagcaa
     9121 gggaaaattt tgttacaata atagacggta ctttttactt ttggtctctc aagagtgtac
     9181 agggacgtgc tgacaaatgt tgcaaaagta cacacagatg atatctgtca ccaagtgtat
     9241 catcaccaaa aataaaaaaa cacaggagaa tgtagatgcc tacaattaac caattggttc
     9301 gcaaaccgcg taaatcaaaa gtagaaaaat ctaaatcacc agctttgaac gttggttaca
     9361 atagtcataa aaaagttcaa acaaacgttt cttcaccaca aaaacgtggt gttgcaactc
     9421 gtgttggaac aatgacacct aaaaaaccta actcagccct tcgtaaattc gctcgtgtac
     9481 gtttgagcaa ccttatcgaa gttactgcct acatcccagg tatcggacac aacttgcaag
     9541 agcacagcgt ggtgcttctt cgcggtggac gtgtaaaaga ccttccaggg gtacgttacc
     9601 atatcgtccg tggtgcactt gatactgcag gtgttaacga tcgtaaacaa ggtcgttcta
     9661 aatacggtac taaacgtcca aaagcataag gaaaggggat aaagagaaat gagtcgtaaa
     9721 aatagagctc caaaacgtga cgtattgcca gatccgctat acaattcaca actagttact
     9781 cgtcttatca accgcgttat gcttgatggt aaacgtggta ctgctgcttc aatcgtttac
     9841 ggtgcttttg agcaaatcaa agaagctact ggcaacgatg cacttgaagt atttgaaaca
     9901 gctatggaaa acatcatgcc tgtacttgaa gtacgtgcac gtcgtgttgg tggttctaac
     9961 taccaagtcc cagttgaagt tcgtccagaa cgtcgtacaa cacttggact tcgttggttg
    10021 gtaacaatcg ctcgtcttcg tggtgaacac acaatgcaag accgtcttgc aaaagaaatc
    10081 ttggatgctg ctaacaacac tggtgcagca gttaagaaac gtgaagatac tcaccgtatg
    10141 gctgaagcta accgtgcatt cgcacacttc cgttggtaag ataggatgcg aaagcgttaa
    10201 gaaagtccca gagaaaatag ggaatcgaag caggttgcgg ttgcaaccaa tgagattcat
    10261 ctttttctcc agacttttag cttgagctca actaaatcat gatgctagga acggtaagga
    10321 tgcaaggtga aaataggaaa ctgacgcagt attcgacgaa tacaaggagt tttatctttt
    10381 tcacgcagca tcccgttcca gctcacatcg gctaactaac tttagcccgg gttcaaatta
    10441 gctaaatcga ttagtattag ctataactca gcttaccatc tcgtaagttg aaaccaacaa
    10501 tagcatgaaa acattgagaa cgggtaggtc ctgcctatcc gtttttatta aaatcgtgtt
    10561 ataatagaat agaaatcaaa aataaatagg agaaacaaac ctcatggcac gcgaattttc
    10621 acttgaaaaa actcgtaata tcggtatcat ggctcacgtc gatgccggta aaacaacaac
    10681 tactgagcgt attctttact acactggtaa aatccacaaa atcggtgaaa ctcacgaagg
    10741 tgcgtcacaa atggactgga tggagcaaga gcaagaacgt ggtatcacga tcacatctgc
    10801 tgcgacaaca gctcaatgga acaaccaccg cgtaaacatc atcgacacac caggacacgt
    10861 ggacttcaca atcgaagtac aacgttctct tcgtgtattg gatggtgcgg ttaccgttct
    10921 tgactcacaa tcaggtgttg agcctcaaac tgaaacagtt tggcgtcaag caactgagta
    10981 cggagttcca cgtatcgtat ttgccaacaa aatggacaaa atcggtgctg acttccttta
    11041 ctctgtaagc acacttcacg atcgccttca agcaaatgca cacccaatcc aattgccaat
    11101 cggttctgaa gatgacttcc gtggtatcat tgacttgatc aagatgaaag ctgaaatcta
    11161 tactaacgac cttggtacgg atatccttga agaagacatc ccagctgaat accttgacca
    11221 agctcaagaa taccgtgaaa aattgattga agcagttgct gaaactgacg aagaattgat
    11281 gatgaaatac ctcgaaggtg aagaaatcac taacgaagaa ttgaaagctg gtatccgtaa
    11341 agcgactatc aacgttgaat tcttcccagt attgtgtggt tcagccttca aaaacaaagg
    11401 tgttcaattg atgcttgatg cggttatcga ctaccttcca agtccacttg acatcccagc
    11461 aatcaaaggt attaacccag atacagacgc tgaagaaact cgtccagcat ctgacgaaga
    11521 gccatttgca gctcttgcct tcaagatcat gactgaccca ttcgtaggtc gtttgacatt
    11581 cttccgtgtt tactcaggtg ttcttcaatc aggttcatac gtattgaata cttctaaagg
    11641 taaacgtgaa cgtatcggac gtatccttca aatgcacgct aacagccgtc aagaaatcga
    11701 cactgtttac tcaggtgata tcgctgctgc cgttggtttg aaagatacta caactggtga
    11761 ctcattgaca gatgaaaaag ctaaaatcat ccttgagtca atcaacgttc cagaaccagt
    11821 tatccaattg atggttgagc caaaatctaa agctgaccaa gacaagatgg gtatcgccct
    11881 tcaaaaattg gctgaagaag atccaacatt ccgcgttgaa acaaacgttg aaactggtga
    11941 aacagttatc tcaggtatgg gtgaacttca ccttgacgtc cttgttgatc gtatgcgtcg
    12001 tgagttcaaa gttgaagcga acgtaggtgc tcctcaagta tcttaccgtg aaacattccg
    12061 cgcttctact caagcacgcg gattcttcaa acgtcagtct ggtggtaaag gtcaattcgg
    12121 tgatgtatgg attgaattta ctccaaacga agaaggtaaa ggattcgaat tcgaaaacgc
    12181 aatcgtcggt ggtgtggttc ctcgtgaatt tatcccagcg gttgaaaaag gtttggtaga
    12241 atctatggct aacggtgttc ttgcaggtta cccaatggtt gacgttaaag ctaagcttta
    12301 tgatggttca tatcacgatg tcgactcatc tgaaactgcc ttcaagattg cggcttcact
    12361 ttcccttaaa gaagctgcta aatcagcaca accagctatc cttgaaccaa tgatgcttgt
    12421 aacaatcact gttccagaag aaaaccttgg tgatgttatg ggtcacgtaa ctgctcgtcg
    12481 tggacgtgta gatggtatgg aagcacacgg taacagccaa atcgttcgtg cttacgttcc
    12541 acttgctgaa atgttcggtt acgcaacagt tcttcgttct gcatctcaag gacgtggtac
    12601 attcatgatg gtatttgacc actacgaaga tgtacctaag tcagtacaag aagaaattat
    12661 taagaaaaat aaaggtgaag actaatccgt cctcactcta gaaggaagtc acttagtggc
    12721 ttccttttgt ctttagaaaa tacctctaaa tatggtaaaa tagtagaaga ataatgtgag
    12781 gaaaatgaat gtcaaatagt tttgaaattt tgatgaatca attggggatg cctgctgaaa
    12841 tgagacaggc tcctgcttta gcacaggcca atattgagcg agttgtggtt cataaaatta
    12901 gtaaggtatg ggagtttcat ttcgtatttt ctaatatttt accgattgaa atctttttag
    12961 aattaaagaa aggtttgagc gaagaatttt ctaagacagg caataaagct gtttttgaaa
    13021 ttaaggctcg gtctcaagaa ttttcaaatc agctcttgca gtcctactat agggaagctt
    13081 tctctgaagg tccatgtgct agtcaaggtt ttaagtccct ttatcaaaat ttgcaagttc
    13141 gtgctgaggg taatcagcta tttattgaag gatctgaagc gattgataag gaacatttta
    13201 agaagaatca tcttcctaat ttagccaaac aacttgaaaa gtttggtttt ccaactttta
    13261 actgtcaagt cgagaagaat gatgtcctga cccaagagca ggaagaggcc tttcatgctg
    13321 aaaatgagca gattgttcaa gctgccaatg aggaagcgct ccgtgctatg gaacaactgg
    13381 agcagatgac acctcctcca gcggaagaga aaccagtctt tgattttcaa gcgaaaaaag
    13441 ctgcagctaa acccaagctg gataaggcgg agattactcc tatgatcgaa gtgacgacag
    13501 aggaaaatcg tctggtattt gaaggggttg tttttgatgt ggagcaaaaa gtgactagaa
    13561 caggtcgtgt tttaatcaac tttaaaatga cggactatac ttcaagtttt tctatgcaaa
    13621 agtgggttaa aaacgaggaa gaggcccaga agtttgacct catcaagaag aattcttggc
    13681 tccgagttcg agggaatgtg gagatgaata acttcacacg cgatttgact atgaacgtac
    13741 aggatctgca ggaagttgtt cactatgagc ggaaggattt gatgccagaa ggtgagcgtc
    13801 gggttgagtt tcatgctcat actaacatgt cgactatgga tgctttgcca gaggtcgaag
    13861 agattgttgc aacagctgct aagtggggac acaaggcggt tgctatcaca gaccatggga
    13921 atgtccagtc ctttccacat ggctataagg cggctaagaa agcgggaatc cagctgatct
    13981 atgggatgga agccaatatc gtggaggacc gtgtccctat cgtctataac gaagtggaga
    14041 tggacttatc agaagcaacc tacgtggtct ttgacgtgga aacgacggga ctttcagcta
    14101 tctataatga cttgattcag gttgcggcct ctaagatgta caaggggaat gttattgctg
    14161 aatttgatga atttatcaat cctgggcatc ccttgtcagc ttttactaca gagttaactg
    14221 gaattacaga tgatcatgtc aaaaatgcca aaccactaga acaagttttg caagaattcc
    14281 aagaattttg caaggatacg gtcctagttg cccacaatgc tacctttgac gttggcttta
    14341 tgaatgctaa ttatgagcgt catgatcttc caaagattag tcagccagtt attgatacgc
    14401 tggagtttgc tagaaacctc tatcctgagt ataaacgcca tggtttgggg cctttgacca
    14461 agcgttttgg tgtggccttg gaacatcacc acatggccaa ctacgatgcg gaagcgactg
    14521 gtcgtctgct tttcatcttt atcaaagagg tagcagaaaa acatggtgtg accgatttag
    14581 ctagactcaa cattgatcta atcagtccag attcttacaa aaaagctcgg atcaagcatg
    14641 cgaccatcta tgtcaagaat caggtaggtc taaaaaatat ctttaagctg gtttccttgt
    14701 ctaataccaa gtattttgaa ggagtgtcac ggattccgag aacggttcta gatgcccatc
    14761 gagagggctt gattttaggt tcagcctgtt cagagggtga agtttttgac gtggtcgttt
    14821 ctcaaggtgt ggatgcggcg gttgaggtgg ccaagtatta tgactttatc gaggtcatgc
    14881 caccggctat ctatgcgccc ttgattgcta aagagcaggt caaggatatg gaggaactcc
    14941 agaccattat caagagtttg atagaggttg gagaccgcct tggcaagcct gttctggcta
    15001 cgggaaatgt tcactatatc gaaccggaag aagagattta tcgtgaaatt atcgtccgta
    15061 gtttgggaca gggtgcgatg attaaccgaa ctatcggtca tggtgaacat gcccaaccag
    15121 caccacttcc aaaggctcat tttcgaacga ctaatgagat gttggatgaa tttgcctttt
    15181 tgggagagga actggctcgt aaactggtta ttgaaaacac caatgccttg gcagaaatat
    15241 ttgaacccgt tgaagtcgtt aagggtgact tgtatacgcc tttcatcgac aaggctgaag
    15301 aaacagttgc tgagttgacc tataagaaag cttttgagat ttatggaaat ccgctgccag
    15361 atattgttga tttgcggatt gaaaaagaat taacatccat actggggaat ggatttgctg
    15421 tgatttatct ggcatcgcag atgctggtgc aacgttctaa tgaacggggt tatttggttg
    15481 gttctcgtgg gtctgtcgga tctagtttcg ttgcgaccat gattgggatt acggaggtca
    15541 atcctctctc tcctcactat gtctgtggtc agtgtcagta cagtgagttt atcacagatg
    15601 gttcgtacgg ctcaggattt gatatgcccc ataaggactg tccaaactgt ggtcacaaac
    15661 tcagtaaaaa cggacaggat attccgtttg agaccttcct tggttttgat ggggataagg
    15721 ttcctgatat tgacttgaac ttctcgggag aagatcagcc tagcgcccac ttggatgtgc
    15781 gtgatatctt tggtgaagaa tatgccttcc gtgcgggaac ggttggtacg gtagctgcca
    15841 agactgccta tggatttgtc aagggttacg agcgagatta tggcaagttt tatcgtgatg
    15901 cagaagtaga acgcctcgct caaggagcgg cgggtgtcaa gcggacaaca ggccaacacc
    15961 cggggggaat cgttgttatt ccgaactata tggatgtcta cgattttacg cctgtccagt
    16021 atccagcaga tgatgtcacg gctgaatggc agaccactca ctttaacttc cacgatatcg
    16081 atgagaacgt cctcaaactc gatgtactgg ggcatgatga tccgaccatg attcgaaaac
    16141 ttcaggattt gtctggtatt gaccctaatg aaattcctat ggatgacgaa ggcgtgatgg
    16201 cactcttttc tgggactgat gtgctagggg taacacctga acaaattgga acgcctacgg
    16261 gtatgttggg gattccagag tttggaacaa atttcgtacg tggaatggta gacgaaaccc
    16321 atccgacaac ctttgcggaa ttgcttcagc tgtctggtct gtcccacggt actgacgttt
    16381 ggttggggaa tgctcaggat ctgattaagc aaggaatagc ggacctatcg actgttatcg
    16441 gttgtcggga cgacatcatg gtttacctca tgcatgcggg tctggaacct aagatggcct
    16501 ttaccattat ggaacgggta cgtaagggtt tgtggctaaa gatttcagaa gaggagagaa
    16561 atgactatat cgaagccatg aaggctaata aggtgccaga gtggtatatc gaatcctgtg
    16621 ggaaaattaa gtacatgttc cctaaggccc atgcggcagc ctacgttatg atggccttgc
    16681 gtgtagctta cttcaaggtt caccatccta tttattacta ctgtgcttac ttctccattc
    16741 gtgctaaggc ttttgatatc aagaccatgg gtgcgggctt ggaggtcatc aagcgcagaa
    16801 tggaagaaat ctctgaaaaa cggaagaaca atgaagcctc taatgtggaa atcgatctct
    16861 atacaactct tgagattgtc aatgagatgt gggaacgagg tttcaagttt ggtaaattag
    16921 atctctactg tagtcaggcg acagagttcc tcatcgacgg ggataccctt atcccaccat
    16981 ttgtagcaat ggatggtctg ggagagaacg ttgccaagca actggtgcgg gcgcgtgaag
    17041 agggagaatt cctctctaaa acagaactac gcaagcgtgg tggactctca tcaaccttgg
    17101 ttgaaaagat ggatgagatg ggtattcttg gaaatatgcc agaggataac cagttgagtt
    17161 tgtttgatga gttgttttaa aaaattgctt aataatctat taaaagaggc taacgtatat
    17221 ccaatagatt tacattagct ttcttttttg ttaaaatagt ctatggaaag agggtgagag
    17281 tatgtcaaag atgagtataa gcatccgtct ggatagtgag gttaaggagc aggcccaaca
    17341 ggtgtttagt aatctgggaa tggatatgac aacagctatt aatattttcc ttcgtcaggc
    17401 aattcaatat cagggattac cttttgatgt tagactagac gaaaatcgga agttgctcca
    17461 agtgttaacg gatttagacc aaaatcgtaa tatgagccag tcttttgaat cagtctcaga
    17521 tttgatggag gacttacgtg cttaagattc gttatcataa acagtttaaa aaagatttta
    17581 agttggctat gaagcgtggt ttgaaggcag aattattaga agaagttttg aattttctgg
    17641 ttcaagaaaa agaacatcct gccagatatc gtgatcattc attgacggca tccaagcatt
    17701 ttcaaggagt tcgtgaatgc catacccagc cagattggct tttggtttat aaagtagaca
    17761 agtcggaatt gattttaaat ttgctgagga caggcagtca cagtgattta ttttaatcta
    17821 ttttaagggg gttctcatga aactaagaat atttgcggaa gataagccgg ctaagaaggt
    17881 atttgaatat caattagaac ttgctgatcg tacaattctt ctatcgacag cactcttgtc
    17941 aggtgctatt gctttagcag gaatcttttc tgctttgaga gaaaaataaa aatagaaaag
    18001 agaaaacaga atggttttac caaattttaa agaaaatcta gaaaaatatg cgaaattgtt
    18061 ggttgcgaac ggaattaacg tgcaacctgg tcacactttg gctctctcta ttgatgtgga
    18121 gcaacgtgaa ttggcacatc taatcgtgaa agaagcttat gccttgggtg cgcatgaggt
    18181 catcgttcag tggacagatg atgtgattaa ccgtgagaaa ttcctccatg ccccgatgga
    18241 gcgtttggac aatgtgccag aatacaagat tgctgagatg aactatctct tggagaataa
    18301 ggctagccgt cttggagttc gttcatctga tccaggtgcc ttgaacggag tggacgctga
    18361 caagctttca gcttctgcta aagctatggg acttgccatg aagcctatgc gtatcgcaac
    18421 tcaatctaac aaggttagct ggactgtagc agctgcagca ggacttgagt gggctaagaa
    18481 agtcttccca aatgctgcga gcgacgaaga agcagttgat ttcctttggg accaaatttt
    18541 caaaacttgc cgtgtctacg aagcagatcc tgttaaggcc tgggaagagc atgcagctat
    18601 cttgaagagc aaggccgata tgcttaataa ggagcaattt tcagcccttc actacacagc
    18661 gccaggaaca gatttaacac ttggtttgcc aaagaaccac gtttgggaat cagctggtgc
    18721 tgtcaatgca cagggcgaag gattcttgcc aaatatgccg acagaagagg tcttcacagc
    18781 gcctgacttc cgtcgtgcag atggttatgt cacttctaca aaaccgctta gctacaacgg
    18841 aaatatcatc gaaggtatta aggtaacctt taaggatgga caaatcgtag atatcactgc
    18901 tgagaagggt gatcaggtta tgaaagacct tgtctttgaa aatgcgggtg cgcgtgcctt
    18961 gggtgaatgt gccttggtac cagatccaag tccaatttct cagtcaggca ttaccttctt
    19021 taacaccctt ttcgatgaaa atgcgtcaaa ccacttggct atcggtgcag cctatgcgac
    19081 tagcgtcgtt ggtggagcag agatgagtga agaagagctt gaagctgcgg gacttaaccg
    19141 ttcagatgtt cacgtggatt ttatgattgg gtctaatcag atggatatcg atggtatccg
    19201 tgaggatgga actcgagtac ctcttttccg taatgggaat tgggcaaatt aaggagataa
    19261 tatgttagga agtatgttcg ttggtctcct agtgggattt ttagcaggtg ctatgaccaa
    19321 tcgtggagag cgaatgggat gttttggaaa aatgtttctc ggttggatcg gagcctttct
    19381 aggtcacttg ctctttggaa cttgggggcc agttttatca ggaacagcta ttatcccagc
    19441 tgttttaggt gccatgattg tcttagctat tttttggaga cgaggaagtt aatttcctaa
    19501 atctgatact caatgaaaat caaagagcaa actaggaaac tagccgcagg ttgctcaaag
    19561 cactgctttg aggttgcaga tagaactgac gaagtcagta acatatatac ggcaaggcga
    19621 cgttgacgcg gtttgaagag atttttgaag agtatgaaac gaaaaggagg ttggtcattg
    19681 tactagcctc cttttgatat gatataatag ttctatgaga ttagataaat ttttagttgc
    19741 ctgtgcggtg ggaagtcgga ctgaggtcaa aaacttgctc aaggctgggc gcgtgactgt
    19801 aaatggtaaa aaagaaaaat cagctaaatt gcagattgat gaaaaaatag atgagattcg
    19861 ctttgatggg caagtgttgg agtatgaaga gtttgtctac tacatgatga acaagcccaa
    19921 aggagttatc tcagcgactg aggatcccaa gcacagaacc gttctggact tgttggatga
    19981 cttggcgcgg agtaaggaag ttttcccagt aggacgcttg gatattgaca cgcatggtct
    20041 tttgctcttg accaatgatg gtcagctggc tcatgttctt ctttcgccca agcgtcatgt
    20101 ggacaagact tatctggcac aagtcaaggg aatcatgacc caagaagatg tggagacatt
    20161 tgctgagggt attcctctca aagactttac ctgtcaaccc gctatactgg agcttgtatc
    20221 catagataca gaaaagaatc aaagccaaat ccgtgtgacc attgcagaag ggaagtttca
    20281 tcagatcaag cgtatggtgg gctactgtgg caaggaagtg gtggacttgc aacgattgac
    20341 tatgggaact ttagtattag atgagaacct ggaacgaggg gaatggcgtc gcttgaccaa
    20401 ggaagaatta gaaattctcc gcgctaatat tatttaattt agtttaatac tcaatgaaaa
    20461 tcaaagagca aactaggaaa ctagccgcag gctgtacttg agtacggcaa ggcgacgttg
    20521 acgtggtttg aagagatttt cgaagagtat aaattcgaaa aaggtgaggg acaatgtcct
    20581 cgccttttat gttttttagt tgcttccttt gtgaaaagag ttataataga ctgtagaata
    20641 aaaggaggaa tctatgaacg cgattcaaga atcatttact gataaactat ttgccaacta
    20701 tgaagcaaat gtaaaatacc aagcgattga aaatgctgcc agccacaacg gaatttttgc
    20761 agctctggaa cgtcgccaaa gccatgtaga caacacacct gttttctcat tggatttaac
    20821 caaggacaag gtcactaacc agaaagcgtc tggtcgttgc tggatgtttg cggctctcaa
    20881 caccttccgc cacaaactca tctcgcaata caaattggag aactttgagt tgtcacaagc
    20941 ccacactttc ttctgggaca agtatgagaa atcaaactgg ttcttggagc aagtcattgc
    21001 gacttcagac caagaattga ctagccgcaa ggttagcttc ttactccaaa cacctcaaca
    21061 agatggcggt caatgggata tggtcgtttc cctctttgaa aaatacggtg tcgtgcctaa
    21121 gtcagtttat cctgagtctg tttcatctag cagcagtcgt gagctaaatg cgatccttaa
    21181 taaattgctt cgtcaagatg ctcaaatctt gcgtgatttg cttgtttctg gtgcagatca
    21241 agcgactgtt caagctaaga aagaagacct cttgcaagaa atctttaact ttcttgctat
    21301 gtcattagga cttccaccac gcaagtttga ctttgcttat cgcgataaag ataacaacta
    21361 caaaagtgaa aaaggaatca caccacaaga gttttacaag aaatatgtca atcttccttt
    21421 agaagactac gtttctgtta tcaatgctcc aactgctgat aaaccttacg gaaaatctta
    21481 cacagttgag atgttgggga atgtggttgg tagccgtgca gttcgctaca tcaacgttcc
    21541 aatggagcgc ttgaaagaat tggcgattgc ccaaatgcaa gcaggtgaga ctgtttggtt
    21601 tggttctgat gtcggccagc tcagcaaccg taaggctggc atccttgcga cagatgttta
    21661 tgactttgaa tcaagcatgg acattaaact tactcaagac aaggctggac gtttggacta
    21721 tagtgaaagc ttgatgaccc acgccatggt cttgacaggt gttgacttgg acgaaaatgg
    21781 taaatcaacc aagtggaagg ttgaaaactc atggggagac aaggttggta cagatggtta
    21841 ctttgttgcc tcagacgctt ggatggacga atacacatac caaatcgttg ttcgtaagga
    21901 attgctgaca gcagaagaac aagctgccta tggagcagaa ccaatcgtac ttgcaccatg
    21961 ggatccaatg ggagccttgg ctgaataaaa gcatagaaaa aaggaatcag attttagaac
    22021 ctggttcctt tttagttgct tgattacatg atgtgaagaa catgtgccac aatacccact
    22081 gcgaagaggg caaggataat agtgattgga gatacttttt tcttaagtaa gtacatgcaa
    22141 agtaaagtaa ggagtagtcc tgataatcca ggaatcaaca tatccaagtt ttgttggaaa
    22201 gtagtaactt tttcaggagt ttgagacaat ccttgtccta cttgtgcgaa tgcttcttgg
    22261 atacctttag acccttctgg caatttatcc caatggatat aagccttttc atctagttga
    22321 actttagaaa catcgaaagc aaatttaata tttacccaac gttgaacaag gacagcaaga
    22381 atgaacatcc caaggataga agctccttta gtgatatctt gaaggatacc accagacata
    22441 tctttagtga tttctgatcc agccttgtat ccaatctctt gaacatacca caagaatgac
    22501 atacgaatca agttccatgc aacaaagaag aggagtggcc ccaagatatt gccagtaagg
    22561 gcaagtgaag caccgagaga tccaaggatt gggcgtactg taaaccagaa tactgggtca
    22621 ccgataccag caagaggtcc catcataccg attttaaccc cttggatagc agcgtcatcg
    22681 atttccacac cgttagcacg ttcttcttca agcgcaagag taacccccat gactggagca
    22741 gctacgtatg gatgagtgtt gaagaactca aggtgacgct caagagcagc gatttgatct
    22801 tcttttttag tatagagttt tttgatagct ggaatgagtg tataagccca gcccaagttt
    22861 tgcatccgtt caaagttcca agacccttgt aagaaggttg aacgccacca aactttttta
    22921 cgatctgatt tagttaattg aagtttttca gtcatgatgt tttcagtcct ttcttatctt
    22981 agtagtcttc taggatatcg ccgattgggt cgttagaagt tgcggctcct ccgccaccat
    23041 ttccaccagt tttagaaagg tgaaggtaga taagagcgat agcaacaccg atagcaccga
    23101 atccgattag agtaatatct gacacagcag cgagaacgaa accaagagcg aagaatggcc
    23161 atacttcacg agttgccatc atgttgataa ccatggcgta accaacggca acgaccatac
    23221 caccaccgat agccatacca tctttgagcc agtctggcat ggcactaagg atactttgta
    23281 cagtttcagt tggtaccata aggagaagag ctgcaggaag cgcgatacga agtccttgga
    23341 aaagtagcgc aatgaaatga gcacgctcca cagcgccgaa gtcacctttt ttagcggcag
    23401 catcagcagt gtgaaccaat ccaactgaaa ttgtacgaac aatcatagtt aagaatagac
    23461 cagctacggc aagagggata gcaaccgctt gggcaacacc gataccagtc ttggtaaagt
    23521 caccaccaag aaccatgata atggcagcag cgacagaagc aagtgcagca tcaggagcga
    23581 tagcagcacc gatatttgac caaccaaggg caatcatttg aagcgatcca ccgaggataa
    23641 tccctgcttc caagtgacct gttacaagcc caataagggt acaggctaca agtggttggt
    23701 gaaattggaa ctggtcgagg atgccttcaa gacctgcaaa gaaggctaca acgactacta
    23761 aaaccataga aataatagac atgtttaaaa tcctttcata aataatggct tatttgacat
    23821 tggctttgtt aatcaagtca aacaaatctt ttttagaatc atttggtact ttacggacat
    23881 caaattcaac acccaagtca cgcatttttt caaatgtagc aacatcttct ttgtccatag
    23941 acaaaacggt attgaccaat gttttacctg ttgagtgagc catagaacca acgttaagag
    24001 tcttgattgg cacgccgcct tcgatggcac gaagggcatc ttgaggtgtt tcaaacaaga
    24061 taagggcatg tgtttctcca aaacgtgggt cttttgaaat ctcaatcagt ttttgaattg
    24121 gaaccacgtt agccttgaca ttacctggag ctgcttgttt aatcaattct ttacgaaggt
    24181 cgtctttagc cacgttatct gaagcaacga tgatacgatt tgcttttgaa tctggagtcc
    24241 aagcagttgc aacctgaccg tgaagtagac gtgtgtcaag acgggcaaga ttgattttca
    24301 atttaccgtc tccgataaca gttccttctg ggatagcagt ttgggcaact ggagcagctg
    24361 cagcgcttgc aacttcttca actggattta gctcttctgg aagagctttg atgccatctt
    24421 tggcttcttt aatgatatta gcagcgactt tttctacacc tgcagcagcg tccatgaggc
    24481 gctctgtgta ggcttgaatc aacatcggta agttaagtcc tgtgatgatg gcaaacttac
    24541 gctcaggatt ttctcccatc acgcgactag cttggttaaa tggagaacca ctccaaaggt
    24601 cagccaaaac tagaacctca tcttctgcgt caaatgcagc aacagcgtta ttaaacttag
    24661 cgtatagatc atcaggacct tcatttggca taaaggtcac aacttgaacc ttttcttgtt
    24721 caccaaagat catagatcct gactgatgaa tacccgcagc aaattcgccg tggctcgcaa
    24781 taatgattcc gatactcatt attgtcattc ctcctttttt gttttagttt tcatttaaga
    24841 aaactttaag actgtttaag tataaaccgt tttcataaaa aaagcaacta tttatcataa
    24901 aaaattaaaa aatttttatt tttgtatgtt gatatgttga gtttttgagg agtaatcttg
    24961 aattcttatt taaaatttaa tataaaaaag ttggaagaag cttccaactt ttaaaatagg
    25021 tttctattag attagtgggt gaagtcgagt accatacgtc cttggatttg gcctttttcc
    25081 atttcgtcga aaatggcaac agcatcttct actggacgtt tttgaacaac tgggactacc
    25141 agaccttctg caccaaattg gaaggcttct tctaagtctt tacgagttcc gacaagagaa
    25201 ccgatgactt ggattccatc gaggactgtt ttaacgatgc tgagttccat catttcagaa
    25261 ggaagaccaa cagcgacgac gcgaccacca gcacgaatgg agtcaacagc ctggttgaag
    25321 gcaactttag acacagcagt tacgacagct gaatgagctc ctccatcagt tttttcttta
    25381 atgagtccag ctacatcttc aacttcgagg ccgttaatca caatgtcagc gcctacttct
    25441 tttgcaaggg caagtttgtc attgttgata tcgactgcga taacatgagc attgaatact
    25501 tttttagcgt attgaacagc gaggttacca agtccaccag caccgtaaag aacaacccat
    25561 tggcctggtt caacttttgc ttctttgata gctttatagg ttgttactcc agcacatgtg
    25621 atagaagaag cttgggctgg atcaagtccg tcaggaactt tgacagcata gtcagcagtt
    25681 acgatacatt gttcagccat accaccgtct actgagtagc cagcattttt cactgtacgg
    25741 caaagggttt cgcgaccagt tgtacagtat tcgcaagtgc cacatccttc aaagaaccaa
    25801 gcaacgctga cgcggtcacc gactttaagg cttttcacat ctggagcaat ctctttaacg
    25861 ataccgatac cttcgtgccc aagaacacgt cctgggactt gaccaaagtc accatgagca
    25921 acgtggaggt cggtgtggca aacaccacag tattcaactt ctacaagtgc ttccccagtt
    25981 tcaagtggac ggagtacttt ttcttcaata gcaacaccag tgctttctgg atttacaaca
    26041 acagctttca tagctatcct ccttgatatt gaccgagaac agggacaacg ggactggatt
    26101 gattttttgt caacagagtc gagtgtgacg agctctcttg tatttatatg tgaagtatat
    26161 cacaaaagaa agccttttcc aaggttttgg agacaaaaag tagaacaatc gattaactgg
    26221 ttgatgagac tgtgaaaaga gcacaaagac cagcttgcaa gctggtcata ctcttcgaaa
    26281 atctcttcaa accacgtcag cttcatctac aacctcaaag cagtgctttg agcaacctac
    26341 ggctagcttc ctagtttgct ctttgattct cattgagtat cagtataggt tataagaata
    26401 agtcttgtag ggttttggca accccatctt ggtcgttggt aagggaaatt tgctcatctg
    26461 cataagggag tagctctgga ttggcatttt tcatggcata acccttccca gcaaaagcga
    26521 gcatttcggt atcattgtgt tcatctccaa aggcaatcaa atcttttttg tcacgattca
    26581 ttatcttgag caagtagtcc aaagcaaagg acttgttgac accttttggg gtacattcaa
    26641 ggatattgag cggacctccc caggtattga tagaaagttg atgctggtag aaggcgttca
    26701 tttcttttgc caaggcatat ttgtcactgg ctctggtctg caagaggata cagttagggt
    26761 ccttggtcac caattcaggc tggaattgat cttcaggctg gaaagcttct acaccaaata
    26821 gtttgggatt ggcaatttct tcattgggat ttgtaatgta gaatttttta cgatattctc
    26881 cagcgataaa atcggcttga atgtcctctg aacgttgaac catatctagc agatattttt
    26941 tgtctacagt caaacacttt tcaaaatccc aaacttggtc tggtaaatga gtaagggatc
    27001 cgttgaagtt aatcatagga gtgtctaagc ccagttcacg gtaaaaatct tttgacatac
    27061 ggtaagggcg acctgtcgta ataataacct gatggccttt ttcagcaact tttttgatgg
    27121 ttcttttggt aaagtcagaa atttgactgt ctgagttgag cagggttcca tccaggtcaa
    27181 ctgcaataat ttttttcgtc atatggacct ttctagtgtg ttttcagata agatgtcttc
    27241 tattataaca gaaagcaggc agaaaagcag attccaaaca aaaaatgaaa ttggattaat
    27301 ttcttaaaca aatcaaacaa agacattgaa aaagtgaatg ataaatgata taattctatt
    27361 attgttcgta aaaattaaaa ggagattgat gatggacaaa ttatttaaac taaaagagaa
    27421 cggtacagac gttcgtacag aggttctcgc tggtttaaca actttctttg caatgagcta
    27481 tattctcttt gtaaacccac aaatactttc acaaacagga atgcctgctc agggcgtctt
    27541 cctagcgacg attattggtg cagtagcggg taccttgatg atggcttttt atgctaactt
    27601 accttatgcc caagcgccag gtatgggact caatgccttc tttaccttta cagttgtatt
    27661 cgggcttggt tattcttggc aagaagccct agctatggtc ttcatctgtg ggattatttc
    27721 attgattatt accttgacaa atgttcgtaa aatgatcatt gaatcgattc ccaatgctct
    27781 tcgctcagct atttcagctg gtatcggtgt cttccttgcc tatgtaggga ttaagaatgc
    27841 tggacttttg aaattcacga ttgatccagg caactatact gttgtaggag aaggggctga
    27901 caaagctcaa gcaacgattg cagcaaactc ttcagcagtt ccaggattgg tcagctttaa
    27961 taatccagct gttttagtgg ctcttgcagg acttgccatt actatcttct ttgtcatcaa
    28021 agggattaaa gggggaatta ttctctctat cttgacaaca actgttcttg ctattgcagt
    28081 tggtttggtt gatttgtcta gtatcgattt tgctaataac catgttggtg cagcttttga
    28141 agatttgaag acaatctttg gtgcagctct tggttcagaa ggtttgggag ctttggtttc
    28201 agatacagct cgcttgcctg aaactctgat ggccattctt gccttctcat tgacagatat
    28261 ttttgacaca attggtacct tgatcggtac aggtgaaaaa gttggtatcg tagcgacaaa
    28321 tggtgaaaat caccaatcag ccaaattgga taaggctctt tactctgatt tgattggaac
    28381 gacagtcggt gctattgcag gtacttcaaa cgtaacgact tatgttgagt ctgctgctgg
    28441 tatcggtgca ggtggacgta ctggtttgac agccttggtt gtagctatct gttttgcgat
    28501 ttcaagcttc tttagcccac ttctagcgat cgtaccaaca gcggctacag ctccaatctt
    28561 gattatcgtt gggattatga tgcttggtag cttgaaaaat atccattggg atgatatgtc
    28621 tgaagcagtt cctgccttct tcacatctat ctttatggga ttcagctact ctatcactca
    28681 agggattgca gttggtttct tgacttacac tttgactaag cttgttaaag gtcaagttaa
    28741 agatgttcat gccatgattt ggattttgga tgccttgttt atccttaact acatcagcat
    28801 ggccttataa tagaatgacc cagggggatt tccccccttt tttaatacaa aggagatagg
    28861 tgatgaaaga gaaaaatatg tggaaagaat tgttgaatcg tgcaggctgg attatggtct
    28921 ttttacttgc cgtcctttta tatcaggttc ccctagtggt tacctctatt ttgactttaa
    28981 aagaagtagc cctgctacag tcagggctga tagttgctgg cctttcaatt gtggttctgg
    29041 ctctatttat tattggagct cgtaaaacgc aattggcaag ttttaatttt tcttttttta
    29101 gagctaaaga tttggcacgt ttgggcttga gttatttagt tattgtcggg tcaaatatac
    29161 ttggttctat cttgttgcaa ctgtcaaatg agacgacaac agctaaccag tctcagatta
    29221 acgatatggt tcaaaatagt tcgttgattt ccagtttctt cttgctagcc ttgcttgctc
    29281 cgatttgtga ggaaatcttg tgccgtggga ttgttcctaa aaagattttc cgaggcaagg
    29341 agaacttggg atttgtagtc ggtacgattg tgtttgcttt attgcatcaa ccaagtaatt
    29401 taccttcttt attgatttat ggaggtatgt cgacagttct atcttggacg gcctacaaga
    29461 cccaacgttt ggaaatgtcg atcttgcttc acatgattgt taatgggatt gctttctgtt
    29521 tgttggctct tgtggtgatt atgagtcgga cattaggaat ttctgtgtaa aagtttttat
    29581 gtaggaaccg acctctttct actagggaaa gatgaatgca atcgtgtcca tctttttctt
    29641 tttatggtaa aatagaaaaa taatatgatg aaaatccttg agggagtgac cgatatgtca
    29701 agtaaagcca atcatgcaaa gacagttatt tgcggaatta tcaatgtaac cccagactcc
    29761 ttttcggacg gtggtcaatt ttttgctctt gagcaggcac tccagcaggc tcgtaaattg
    29821 atagcagaag gagccagtat gctcgatatc ggcggagaat cgactcggcc gggcagtagc
    29881 tatgttgaga tagaagagga aatccagcgt gttgttccag tgatcaaagc gattcgcaag
    29941 gaaagtgatg tcctcatctc tattgatact tggaagagtc aagtagcaga ggctgctttg
    30001 gctgctggtg ccgatctagt caatgatatc actggtctca tgggcgatga aaaaatggct
    30061 catgtggtag ctgaagcgag agcgcaagtg gtcatcatgt ttaatccagt tatggcgcga
    30121 cctcaacacc ctagctcgct catattccct cattttggat ttaatcaaac ttttacagta
    30181 gaagagttag ctgactttga aatattgcca atcgaagact tgatggtggc tttctttgaa
    30241 cgagcactag cgagagcggc agaagctggt attgcaccag aaaatatcct gttggatcca
    30301 ggaattggct ttggtctgac caagaaagaa aatctgcttc ttttacggaa cctggataaa
    30361 ctacatcaga aaggctatcc aatctttctc ggagtttcgc gcaagcgatt tgtcatcaat
    30421 atcctagagg agaatggttt tgaagtcaat cctgagacag agcttggttt ccgaaatcgg
    30481 gacacggctt cggctcatgt aactagtatc gctgcgagac agggtgtaga agtggtgcgc
    30541 gtgcatgacg tagctagtca caggatggca gttgaaattg cctctgccat tcgtctggct
    30601 gatgaagcgg aaaatttaga tttaaaacaa tataaataag atgaaagaaa ttgaaaacaa
    30661 tcagtggatt gctaactacc ggacggatca accgcatttt ggtttggaac gaatggtgga
    30721 actgttagct ttgcgtggca atccccatct caaactcaag gtcctccata tcggagggac
    30781 taacggcaag ggctcgacta ttgctttttt gaaaaagatg ctagaaaagc tagggctgag
    30841 agttggcgtg tttaactcgc cctatctcat tcattacaca gaccagatta gcatcaatgg
    30901 ggaatcgatc tcagaagcga ggctagaagc tctcatggca gactatcagt ctttgatggg
    30961 gggagaagcg gtcgccaatt tacagggcac aaccgagttt gagattatca cagccctggc
    31021 ctatgactac tttgcctcag agcaagtaga tgtggccatc atggaagttg gcatgggtgg
    31081 acttttggat agtaccaatg tctgtcagcc cattttgaca ggaattacaa ctattggctt
    31141 ggatcatgtg gccctacttg gtgacacctt ggaggccata gcagagcaga aggcaggtat
    31201 tatcaaacaa gggatgccct tggtaacagg gcgtattgct ccagaagcct tggctgtgat
    31261 tgaccgcatt gcggaaggga aagatgcgcc gagacttgcc tacgggacag attatcaggt
    31321 tcgtcatcaa gaaagtgtgg tgacagggga agtctttgac tatacaagtg ctgtcagaca
    31381 aggtcgcttc cagactagcc tgcttggttt gtaccaaata gagaatgctg ggatggccat
    31441 agctttactt gatacttttt gtcaagaaga tggtcgagag ctagcaagca atgactttct
    31501 tggtcaagcc ttggaagaaa caagttggcc agggcgtttg gaaatcgtgt caagagatcc
    31561 cttgatgatt ttggatggag cccataatcc ccatgctatc aaggccttgt tggtaacctt
    31621 gcaagaacgt tttgcggatt atcataagga aatcctcttc acttgtatca aaaccaaagc
    31681 cttggaggat atgttggact tgctgggagc catgccagat accgagctta ctctaacaca
    31741 ttttgcggat agtcgggcga cggatgaaag cgtgctgaaa gaggcagcta agtctagaaa
    31801 tctcagctac caagattggc atgattttct agagcagaat ttgacagata aaaaagaaga
    31861 gaaacaaaca gttaggattg tcacaggttc cttgtatttc ttgagccaag tgagggccta
    31921 tctgatggag aggaagaacg agaatggata cacaaaagat tgaagcagct gtaaaaatga
    31981 ttatcgaggc tgtaggagag aacgctaatc gcgagggctt gcaggaaaca cctgctcgtg
    32041 tagcccgtat gtatcaagag attttttcag gtcttggtca aacagcagag gaacatttgt
    32101 caaaatcctt tgaaattatt gacgataata tggtggtaga aaaggatatc tttttccata
    32161 ccatgtgtga acaccacttc ttgccatttt atggtagagc gcacattgcc tacattccag
    32221 atggtcgtgt ggcaggcttg tctaagctag cccgtacggt tgaagtttat tcgaaaaaac
    32281 cacaaattca agaacgtttg aatatcgaag tggccgatgc cttgatggac tatctaggtg
    32341 ctaaaggagc ctttgttgtc attgaggcgg aacatatgtg tatgagtatg cgtggtgtta
    32401 gaaaaccagg tactgcaacc ttgacgacag tagctcgtgg tctatttgaa acagataagg
    32461 acctccgcga tcaagcttat cgtttaatgg ggctataaaa agaatccgct ttaagcggat
    32521 ttttctagaa aggaatcatt atggatcaac tgcagattaa ggatttggaa atgtttgcct
    32581 atcatggtct ttttcctagt gagaaagaat tggggcagaa atttgtcgtt tcagccatcc
    32641 tatcctatga tatgaccaag gcagctacag acttggattt aacagcctct gtccattacg
    32701 gagaattgtg tcagcagtgg acgacttggt ttcaggaaac gagtgaagat ttgattgaaa
    32761 cggtagccta taaactggtg gaacgtacct ttgagtctta tcctcttgtc caagaaatga
    32821 agttggaact gaaaaaacct tgggcaccgg tgcatttgtc actagatact tgctcggtaa
    32881 ccattcatcg ccgcaagcaa cgagccttta tcgccctagg aagcaatatg ggagataaac
    32941 aagcaaactt gaaacaagcc attgagaaaa tgcgagctcg tggcatctat attctcaaag
    33001 agtccagtgt cttagcgacg gagccttggg gtggagtgga gcaggatagc tttgccaatc
    33061 aagtggttga ggtggaaacc tggctaccag cacaagactt gttagaaacc ttgttagcca
    33121 ttgagtcaga gctgggacgg gtgagagaag tgcattgggg acctcgtttg attgatttgg
    33181 acttgctctt tgtggaggac cagatccttt atacagacga cctcatattg cctcatcctt
    33241 acatagcgga acgccttttt gtccttgagt ccttacagga aattgcgcct cattttatcc
    33301 atccgatatt aaaacaaccg atccgcaact tgtatgatgc tttgaaaaaa tagaaaaact
    33361 ctagttttca gttacttgca actgaaggct agagttttta aaataggtca ttttcttctg
    33421 ggaggaggat agtttctcta ccgtccatgt ctaaaaccag tactcttggg ggataacgag
    33481 ggtcgaaagg gtggttaaag tcaaaatcaa tggctgtagg gaggtgttga cttgaaaagt
    33541 ggaaggtaat ctttccttgg ttattaagca attgaaactc gagttcttct tccaattcaa
    33601 agacattttt taagaaatga tcgatgatat accaaaaaga gtcaatgatg tcatcgggca
    33661 agctggtaac aatgccaaaa ctagcagacc gcatgtgggt attggtaaaa gccatatctc
    33721 tgtccccttt cttttccctt atcatacagc aaataggatt aaaaatcaag aaaaggtgat
    33781 tttttgaaaa ggattttaac tacaaggaaa aagaaggaaa aaaattccca aaaatctacc
    33841 gaagttaata ggtaaattcc caaattaact tgattatata actttcagtt actttgagaa
    33901 gttaccgaaa aatatttttc atatctattg acttttaggg gtgaaatttg gtatgatagt
    33961 aggcggtatt gtttacccca tttgaaaggc cccggaacct tccaaatact tttcgatggg
    34021 aaggaacacc catcaccgta aacaaaaatc gaactatata taggagaaat catgaacaaa
    34081 acaacattta tggctaaacc aggccaagtt gaacgtaaat ggtacgtagt tgacgcaact
    34141 gatgtaccac ttggacgtct ttctgcagta gttgctagcg tacttcgcgg aaaaaacaaa
    34201 ccaacattta caccacacac tgatacaggt gacttcgtga ttgttgtcaa tgctgaaaaa
    34261 gttaaattga ctggtaaaaa agcaactgat aaaatctact acactcactc aaaccaccca
    34321 ggtggattga aacaaatctc tgcaggtgaa cttcgttcta aaaatgcagt acgtttgatt
    34381 gagaaatcag ttaaaggtat gcttccacac aatacacttg gacgcgctca aggtatgaag
    34441 ttgaaagtat ttgttggagc tgagcacact cacgctgcac aacaaccaga agttcttgac
    34501 atttcaggac ttatctaagg aaaggaacaa taaagtatgt cacaagcaca atatgcaggt
    34561 actggacgtc gtaaaaacgc tgttgcacgc gttcgccttg ttccaggaac tggtaaaatc
    34621 actgttaaca aaaaagatgt tgaagagtac atcccacacg ctgaccttcg tcttgtcatc
    34681 aaccaaccat tcgcagttac ttcaactgta ggttcatacg acgttttcgt taacgttgta
    34741 ggtggtggat acgctggtca atcaggagct atccgtcacg gtatcgctcg tgcccttctt
    34801 caagtagacc cagacttccg cgattcattg aaacgcgcag gacttcttac acgtgactca
    34861 cgtaaagttg aacgtaagaa accaggtctt aagaaagctc gtaaagcatc acaatttagt
    34921 aaacgttaat tcgaaagaat tactatactt atacagagca cctttcgggg tgttcttttt
    34981 ttatactttc ttactaaatt ggtgcaattg acacagttgt tgcgacttta gtcgcttaca
    35041 aatgtggctg caacctgaca aggtcagttg cctcaaaacg ttaatcaata cgattatatc
    35101 aatgtttcaa agcactcaaa agtttacctt atgggtgctt ttttcgtgct tttttgaaaa
    35161 gtttacctta tttttatagt aatgtgaaat aagaatagga caaatcgagg atagtcaaat
    35221 cgaattctaa caatgtttta gaagtagagg tgtactattc tagtttcaat ctactatatt
    35281 atggataatg caagattcca tagaatgggg aagctagaac tcttgtgtga agagtttggg
    35341 tataaacttt tacctcttcc tccctactca cctgagtaca atcctattga gaaaacatgg
    35401 gctcatatca aaaagcacct caaaaaggta ttaccaagtt gcaatacctt ttatgaggct
    35461 cttttgtcct gctcttgttt caattgacta taaatcgcta taagaaaaaa tcattttatt
    35521 ggtataaaga agtgattgaa agtaatggcg aaacgttaga taattagttc ataaaaaaca
    35581 agtttagatc ctcatctaaa cttgtttttt taaaattgaa ggatgatttt ggaggatata
    35641 agatgacatt gactattcat gtatattata aaggtgatgg agatagtgca ataaagtttg
    35701 caagagaaat gcttgatagt ggccttgttg aagaaattag aaatcaaaaa ggtaacttga
    35761 aatacgaata ttttttacca attgaaaaag agggaactat tctccttatt gatcagtgga
    35821 taaatcaaga agctttagat gaacatcatc aatcaaagac aatgcagaaa atcttagatt
    35881 taagaaaaaa atatcacttg cagatgcaag ttgaacgtta tatagaagat gattctggga
    35941 tgccagaaag cgataagtcg tttatagaca cagggaatta gaattattgt gagagttttt
    36001 gtagtttgaa agtattgaaa atcgtaaaga tatcattacg agaatacaag aagatattgt
    36061 tctatttcca ttaaaaatta cttctttcct taacaataag gaaaaagaat gcgttttcat
    36121 cttttgattt gaggtttata ataagagtat caaagtcagg aggaaaaaag atgactactt
    36181 ttaaagatgg atttttatgg ggtggtgctg ttgctgctca tcaacttgaa ggtggatggc
    36241 aagaaggtgg caagggaatt agtgttgctg atgttatgac tgctggtcgt catggagtag
    36301 ctcgtgaaat tactttggga gttttagagg gtaaatatta tccaaatcat gaggcgatag
    36361 atttttatca tcgttataaa gaagacatag cactttttgc tgaaatggga ttcaagtgct
    36421 tccgtacctc tcttgcatgg acacgtatct ttccaaaagg tgatgagtta gagccgaatg
    36481 aagaaggatt acagttttat gataatcttt ttgatgaatg cttaaagaat ggtattgaac
    36541 ctgtcatcac tctatctcat tttgaaatgc cttatcactt agtgaccgaa tatggtggtt
    36601 ggaaaaatag gaaattgatt gatttctttg ctcgttttgc agaagtcgta tttaaacgtt
    36661 acaaagataa ggttaaatat tggatgactt tcaatgaaat caataatcaa gcgaattatc
    36721 aggaagattt tgcaccattt actaactcag gtattgtata tgaggaaggt gataatagag
    36781 aggcaattat gtatcaagca gcacattacg aattagttgc ttctgcacga gctgtaaaaa
    36841 ttggtcatga gattaatcca gattttcaaa taggttgtat gattgcgatg tgtccaattt
    36901 atccagttac ttgcaatcct aaggatatct taatggcaat gaaagctatg cagaagcgtt
    36961 attattttgc tgatgtgcat gttttaggta aatatcctga gcatattttc aagtattggg
    37021 aacgaaaagg tatttcagtt gattttactg accaggataa agaagattta cttggtggga
    37081 ctgtagatta cattggtttc agttactata tgtcctttgc tatagactct catcgtgaaa
    37141 ataatcctta ttttgattat cttgaaacag aagatttagt gaaaaataat tatgttaagg
    37201 cttctgaatg gaagtggcaa attgatccag aaggtttgcg ttatgcgtta aattggttta
    37261 cagaccacta tcacttacca ctctttattg ttgaaaatgg ttttggagct atagatcaag
    37321 ttgcagcaga tggtatgata catgatgatt atagaattga atatctaggt gcccatattc
    37381 gtgaaatgaa aaaggctgta gttgaagatg gtgttgattt aatgggttat actccatggg
    37441 gatgtattga tttggtttca gctggtaccg gtgaaatgcg gaaacgttat ggctttattt
    37501 atgtagataa agatgataat gggaagggaa gttataatcg ttccccgaaa aaatcttttg
    37561 gctggtataa ggaagttatt tcatctaacg gtgaatcagt agaatagtat aaacaaactg
    37621 agattattct cagtttgttt agcatattgg ggtgacatat gattggaagt attgcagcaa
    37681 ttttaacaac atttgcattt ttaccacaag tttttcgtgt agttaagaca aaggacacag
    37741 gatctattgc tctaggtatg tatgttatgc aagttattgg tattgcattg tggcttgctc
    37801 atggtattcg tataggagac ttgcctttga ttttagcaaa tagtgtttca tttctactct
    37861 caggaataat tttattttat aaattgaagt ataaataatt tttttccata tcatttagga
    37921 aaatgaggga attgtcattg gaaacgcttt attttataat aaaaatagat aacaaataag
    37981 ccaacttagc acagctttga ttggatttaa gaaaaggaga aaatattatg gcaaaagctt
    38041 taattatttg tgcagcagga atgtcttcat cacttatggc acagaaaaca acagagtttc
    38101 taaaaggaaa agggaaagat attgtagttg atgctattag tgctactgaa ggagggaaag
    38161 ctatcgcagc agcagctttt gatttgtatc ttgtcagtcc tcaaacaaaa atgtacttta
    38221 agcaatttga agaggctggt tctaaagcag gaaaaccagt tgtacaaata cctcctcaag
    38281 cctatattcc tattccaatg ggaattgaaa aaatggctaa tttgattctt gagaatattt
    38341 gatgaacaca tgaaatgtca ataaatattt aagttaagat aaattgtttt gagtttgata
    38401 taaattgatt taagtgtata atatgcttca atatatgaaa acatacgttt taggagggaa
    38461 tgaatgtgtt aaacattaaa gagaaagata tccttcaatt tttaataaaa aataaggaac
    38521 gttttgttac aagcaaagaa ctggcagaat atttatcctg ttcagataga actgtccgta
    38581 atgtactgaa attaattgag aagacaatga ttattcaagg agtccggttg atttcaaaac
    38641 aaggtcaagg gtatcaaata ttttttgaga atcaaggcgc ttaccaggag tttagacaaa
    38701 cttatgaact ggaagaagac tatacgaaaa ctgcggtttc aaaaggtgat gatcgtttag
    38761 tatttatctt aaataaatta ttatttgagc aagttccagt tctttttgat gatttagctg
    38821 atgaacttta cgttagccgt tcaacattat ctcatgattt cagaaagata cgagtgatgt
    38881 tatcagagta taatttgtcc attgagagta gggcgaataa aggagtttat gtctcaggcg
    38941 aggaacgtga taaacgacgt tttatcataa attatttcct agagaatcaa ttttttaaaa
    39001 caatacattg ctatgtaaaa tttaactttt ttgatcaaac tgttccatta gaagaatttg
    39061 ctagaattgt tttggatgaa tgccaagagg ccaacctaaa actatctgat tttgtactac
    39121 agaatttagt agttcacata gcattgtctg tgattcgttt aaaatccgga tttgaaataa
    39181 aaaatataga ctgtcagatg actgatgatg caatagagag aaaggttgcc caaagaattc
    39241 tttctaaagt aagagaagta acaaatcagg aatttccagt tcaggaaata gactacatta
    39301 ctttgcattt gttagctaag agtcaacaat gtcaaaaaaa tcagaagaat atttctgaag
    39361 aagttttaaa aaaatcttta cttaaaacat ttcagaattt aggattagat gatatgtata
    39421 acttttcttc agatttccaa ctaattgaag gattgataac tcatctaatg acactacaag
    39481 tacgtttaga aagccggatt accttaaata accctttggt agatgaaatt aaacagaatt
    39541 atagtgatat attctttatg actagagaaa ttcttgcaaa tatggacaag ttcttggagt
    39601 ggacaatatc agatgatgaa gtagcatatg taagtcttca ctttttagct gctatggaac
    39661 gtagcaaaga gagtactaag tttaatattc ttgctatttg tgcaacagga tttggtgcag
    39721 cccaaatgtt aagaaatcgt ttggaaacgg agtttgggaa gcgagtagaa gttgttgatg
    39781 taataggcta ttatgaattg aatcaagaaa aacttaaagg aatagatttt atagtgtcag
    39841 ctgttgattt atcaaatcta tattttcaaa tcccagtttt taaagttagt gtgtttctta
    39901 agagtgatga gatggaaatg attagaaaag ctatggatca gatgcaagtt tctagttatg
    39961 tacaatcatc aaagattaac aaatttgaaa acaacggctt tagacagtat tttagtaaag
    40021 aaaacttttt aatatgtact gaatcagaca aggtaaattt acttgaaaag atggttgaga
    40081 gtttatctgt tggtgaatca aatgagtttg aacaatcttt actttatgga attaaacagc
    40141 gcgaagaatt aagttcagtt gttttttctg aaaaaattgc agttccgcat cctattcagt
    40201 cctttggtac agagggaaag gtttcggttg caatttgtaa agattcgtta ctctgggata
    40261 atcaaagttc acatgtccag ctagtatttc ttctatctcc atcaatatat gggaacgaag
    40321 gtttggcaac tgtgactaaa aagatagtct ctctgacaga gaatgatgaa ttgcaaaatc
    40381 agctaatatc ttgcaataac tttgaagatt ttattaacat atttgagaag ataaaatagg
    40441 aggttcatat gaatcaagag gaactgcagg ttgcagcatt tgaaattatc ctgcattcgg
    40501 gaaatgcaag gtcagaaata catgaagcat ttgcaaaaat gagagaaggt agttttgatg
    40561 atgcagaaag taaattaaat caatcaaatg agataattct tgaagcgcat catgcacaaa
    40621 ccaaactctt gcaagaatac gcaagtggag tggaaattaa aattgaaatt atcatggtac
    40681 atgctcaaga tcatttaatg accactatga cattattaga agtagcaaag gaaatgttag
    40741 ctctttataa gaaagttaac taaatatgtg ttgttatata aggaggaaat gatatggata
    40801 aaaaagaacg acaaaagatt gaacaacaac gtcgagagat ggctctaact aatacttttt
    40861 tcaatcgata tcttcttcta cgttattcaa tagcactctt cttttttggg aatatttatt
    40921 ggcttttgag tcagtttatt agtccctcgc ctattattat tttccccatt atgctcatag
    40981 tattttctat tttagcaact gttgaacaat ttaaactcta tggaaatagg aaagaaaagt
    41041 tgggaataac actaatgttt gttagaatac aaatgcttat ttcaatagga ttacttgttt
    41101 taacttggac aagttggttt aagaatcttt ttccgatttt tgaaaataac caagtagcac
    41161 gtttatttgt ttttgtagtt cttctattag gtttagttct gagtttgtta gacattagaa
    41221 gaattaaaaa aatttataaa cgaacagata aggcttatca acaatttgtc caattggaaa
    41281 agaactcact tagtttataa tcctgatcta gtataatttg tatttgaaac tagtctttag
    41341 aaagtaaaaa ttattatgtt ttattaagga ggtctattta tggatcaaca aaacgggttg
    41401 tttggttttc ttgaaaacca tgttatggga ccaatgggca aacttgctca gtttaaagta
    41461 gtacgtgcta tcacggctgc aggtatggct gctgtaccat ttactattgt aggatcaatg
    41521 tttttggtat tcagtatttt gccacaagct ttctcatttt ggccaattgt ggcagatatt
    41581 ttctctgctt catttgataa attcacatca ctttacatgg ttgcaaacta tgcgactatg
    41641 ggttctctat ctctttattt cgttctatca cttgcatatg aattgacaaa aatttatgca
    41701 gaggaggaag aactcaatat gaatcctctt aatggtgcct tgcttgcctt gatggctttt
    41761 gtcatgacag taccgcaaat catttttgat ggtggaatga tgaagactgt gacaagtcta
    41821 aaagaaggag cagtaattgc agatggatgg gcaatgggaa atggagtcgc acgttttggg
    41881 acaacaggga tttttaccgc aatcattatg gcaattgtga ctgttcttat ttatcgtatg
    41941 tgtgttaaac ataattgggt tattaaaatg cctgaagctg ttccagaagg agtttctcgt
    42001 ggatttaccg ctttggttcc gggatttgtt gttgcatttg ttgttatctt tatcaacggt
    42061 cttcttgtag caatgggaac agatattttt aaagtcattg caattccatt tggttttgta
    42121 tccaatctga ctaattcgtg gattggttta atgattattt atctattgac tcaactactt
    42181 tggattgtag gtatccacgg tgcgaacatt gtttttgcat ttgttagtcc aattgctctt
    42241 gctaacatgg ctgaaaatgc tgctggcggg cacttcgctg ttgcaggtga attttctaat
    42301 atgtttgtaa ttgcaggtgg ttctggtgca actttaggac tatgtttata tattgctttt
    42361 gcctctaaat ctgaacagct taaagcaata ggacgagcat ctgtagttcc agccttattt
    42421 aatattaatg aaccattaat ttttggatta cctattatct ataatccagc cttggctata
    42481 ccatttattt tagcaccaat ggttactgct actatttatt acgtagcgaa ttctctaaac
    42541 tttattaagc caattatcgc acaggttcca tggccaactc cagtagggat tggagctttc
    42601 ttagggacag cagatcttcg agctgtatta gttgctctag tatgtgcatt tgcagcattc
    42661 ttagtctatc ttccattcat ccgtgtatat gatcaaaaat tggtgaaaga agagcaaggt
    42721 atctaataag taaaaaagta tactcgatga tagaatccat atagtttaaa taatgagcgt
    42781 taacattagc agtgaagagg tctttttaag atattcttct ctgctttttc atattttttc
    42841 gctatgtagg gtatgaagca aaatagaaag tagttcgaat agttgataaa cagggaattg
    42901 ctgttattta aaaaaacaat aaagtaaagt atgttatcgt agaatacgat acaatagtaa
    42961 aaaagcaaaa agaatgcgtt ttatatttgg gtttgaggtt tataaaatag gataagcgtg
    43021 ctgaaaactt agatatgaca aacaaaataa aatttttaaa agctacagag aaacatggta
    43081 taatgtatct aaaggaggta gcatatgtcc ttagatatag ataaagaaaa gatgacaatc
    43141 atgggaatag cttttgaaaa ccgatccgtt tttaagagtg tttggtatgc tttaagtaca
    43201 aatatgattg aaggatggcg acctacggtt agtgatgttg aaaaattacg agatgaagct
    43261 cttgctctgg ggatgactta atgaagcgca actgttatga gtcttatgac tatgttgatc
    43321 cggataattt atataccgat ccgggttctt cagttctaag aaataagcag gaaggaaaaa
    43381 agcacgcgag cttgaatatc gtatgattgc tagtaagagt ttgaaattat ttatcaatcc
    43441 tatcttagtt aatagtttaa aagtagtttg tttcacaact gcataagctt agaaaagttg
    43501 aaaagtgata actctttaat aaactcttgg tttgttaaaa gatattttgt ccactacatt
    43561 tttttcaatt gactattatt tgtcatagat ataacagata gaacattgca ttcagaataa
    43621 tgaaaaacgc atcaaaaatg gtgcgttctg tttttatgct accataaaag gaggaaaaca
    43681 aattgaaaat ttttaaggga gagttttatc gaatctctgt attaacagac aagctagtaa
    43741 ggttagaata ctctcaaact ggaagttttg aggatagaac gacacaactt atctataata
    43801 gagattttgg ccaaatttcg ttagattata tcgagacatc aaacgtacta gatattatga
    43861 cggactattt tcatctgcac tttaataaag gagaatttaa cgccgaaaat ttatttatag
    43921 aattaaaagg aaattttgcc gtatatggta gtcgctggta ttttggtgaa tctattgaaa
    43981 cgttaaaagg aacagctcgg actctggata aggcagatgg agcaatctcg ttagaagatg
    44041 gaattattag ccgaaatggt atagccttat tggatgattc tcaaggattt atttgggatg
    44101 aacaatctgg ttatattgag agagaaaatc aaattgacct gtatttcttt gcctatgggc
    44161 atgattatag aggagcaatc agagattttt accatttgac tggttcaaca cccttgttgc
    44221 caagatatgc tttaggcaat tggtggagta gatattggcc ttatacgtcg gatgaatact
    44281 tggatttaat agacagattt gaaacagaga aaattccatt atctatcggt gtgttagata
    44341 tggattggca tataactgac attccagctc gctttggaag tggctggaca ggatatagtt
    44401 ggaatagaaa cttaatacca aatccagaac agttattgca acaacttcat gatagaaagc
    44461 taaaactctc cttaaatgtc catcctgctg atgggatacg ggcttatgaa gaagcttatc
    44521 ctcaaatcgc aaaacggttg gggttaaatg tagaactaga agaacctgct atttttgatt
    44581 tttttaatcc ctcttttagg gaagcctact ttaaagatgt tcattatgaa ctagaaaagc
    44641 agggagtaga tttttggtgg attgactggc aacaagggac acaaggtatg ctagatccac
    44701 tttggctttt aaaccattat cactatcagg atagttgtaa aaatgcagaa ggtggtttga
    44761 ttttatcaag atatgcaggt cctggtagtc accgctaccc tgttggtttt tcaggggata
    44821 ctattattag ttggaattcc ttaagatttc aaccctattt tacagcgaca gcatctaata
    44881 tcggttatag ttggtggagt catgatatcg gtggacatat gttgggggat tatgacgaag
    44941 agctacaaac tagatggcta cagtttggtg tttttagtcc gattactcga ttacatagtt
    45001 ctagaagtcc ttttaatagt aaagaacctt ggtttttttc agaaacaaca tctaagatta
    45061 tgaagaaata ccttcgtttg agacatcaga tgattcccta tctatatacc atgaatgtac
    45121 agacacatga ggaaggtgcc ccattaatca gtccaatcta ttatttctat ccagagaatg
    45181 atgagagcta taatgttcca aaccaatact tttttggaac agaactgatg gtggctccca
    45241 ttgtagaaaa gatggatttg acattccaat ctgcaaaagt agatgtatgg ttccctgaag
    45301 gtgaatggta tgacttcttt tcagagaaaa aatacacagg tggtgtgaag ttaagtgttt
    45361 atagggacat ctcgactacg cctgtgtttg caaaaagtgg tgcaatcatt cccttggttg
    45421 gttctgagat aggtatgggt gttgatttac ctgaagttgt agattggtat gtattcccag
    45481 gaaaacaaca ttcttttgaa atgcttgaag atcaaaatgg tcaaagatat aaaacaagat
    45541 tatcaatcga ttgggaaatg ggaatggtag agttagcatt acaaggagat tctagtatcg
    45601 ttccaagcaa tagaaaacat agaattcatt ttaaaggaac gaatgtgtct ataattgaat
    45661 tgccaaataa gaatgataca gctaaatttg aatggaaaga taataaaagg acatctttaa
    45721 atgacgaagt ttttagacta ctaaagacag cttctcttcc atatgaatta aaagatagat
    45781 tgttaaatca attcatcaat gcgaaaaact ctcatgactt aatgaatatc ttgcatcatc
    45841 aggataagga attgagaggg cgtttgttgg aaatgatatt tactagccaa aactaattga
    45901 aataattaga aagtcatttg tttagtgtat atgaagattc tgtatggtat tcgttatcac
    45961 tggtcacatt gaaattgtga aataagcacc tacaacatag actttacaac acttcatgtt
    46021 tcatatcatg gggtgttttt ctatttaagt caaagcccat caaaaaatct cactattcat
    46081 caggttaaag gaaacctaat catagtgaga ttgtgaatta tagtagagtt tgtatcgcct
    46141 cttcaatttg ttttgggtct gtttttgaag aaaagcgttc aaagacttgc ccatctcgac
    46201 cgatgagaaa cttagcgaaa ttccattcga ctcgttttcc tagtgggccg gatttctggt
    46261 cttgtaacca gacatagaga gggtctgctt ccttaccgtt gaccttaatc ttggcaaaac
    46321 gtgggaaggt ggtttgaaaa tgtaggctac agaaggcgtt gatttcctct gcgctgccgg
    46381 gtgcttgtcc cataaactga ttgcaaggga aatccaatat ttcgaagccc tgatcttgat
    46441 agcgttcata gagttcttga agtccctggt actggggcgt taaaccacat ccagtagcag
    46501 tgttgacaat caagagaact ttgccacgat agctatccaa gggagttgct tggttgtttt
    46561 ggttcaaaac ggaaaaatca taaagtgaag tcatgagggc ctcttttctt tttctacatt
    46621 gtaacagttt ttactttttt cgtcgagtcg gatggaagat attgttgaag aataagcctt
    46681 caatatgata gaaaccgttt acaaataact ttaaaaatgg tatgatttaa ttaaaccggt
    46741 ttacttagtt gctataaatc tctaatttga gatttagttc ctattgattt tacaatatgt
    46801 ttattggagt gtatacatgc aaacaaaaac aaagaagctc attgtgagtt tgtcttcact
    46861 tgttttatca ggatttttat taaaccatta tatgacaatt ggagcggaag aaacgactac
    46921 gaataccatt cagcaaagcc agaaggaagt tcagtatcag caaagggata caaaaaattt
    46981 agttgaaaat ggtgattttg gtcagacgga ggacggaagc agtccgtgga caggaagcaa
    47041 agctcagggg tggtcagctt gggtagacca gaagaatagt gcagatgcct caactcgagt
    47101 cattgaggct aaggatgggg ctatcactat ctcaagccat gagaaattaa gggcagcgct
    47161 tcaccgtatg gttcctattg aagctaagaa aaagtataaa ctgcgtttca agattaaaac
    47221 agataataaa atcgggattg ccaaagttcg tatcattgag gaaagtggta aggacaagcg
    47281 attgtggaat tctgcaacga cgtcaggaac aaaggactgg cagaccattg aagcagacta
    47341 tagcccgact ttagatgttg ataaaatcaa gctggagtta ttctatgaaa caggaactgg
    47401 gactgtttcc tttaaggata ttgagctggt agaggtagca gaccagcttt ctgaggattc
    47461 tcaaacagat aaacagcttg aggaaaagat tgatttacca attggaaaaa aacatgtttt
    47521 ttctcttgcg gactatactt ataaggtaga aaatcctgac gttgcttcag tcaaaaatgg
    47581 aattttagaa cctcttaagg aagggacaac caatgtcatt gtcagtaaag atggcaagga
    47641 agtgaaaaag attcctttga agattctagc ctctgttaag gatgcataca cagcccgttt
    47701 ggatgactgg aatggcatca tcgctgggaa tcaatactat gattctaaaa atgaacagat
    47761 ggccaaatta aaccaggaat tggaaggaaa ggtagctgat agcctatcta gtatttcaag
    47821 tcaggcggac cgcacctatt tgtgggaaaa attttcaaat tataaaatgt ctgcaaatct
    47881 gactgccact tatcggaaat tggaggagat ggccaagcaa gtgaccaatc cttcttctcg
    47941 ttattatcaa gatgaaactg tcgttcgaac agtcagggat tccatggaat ggctgcataa
    48001 atatgtctac aatagtgaaa agagcattgt tgggaactgg tgggattatg aaatcggtac
    48061 acctcgtgcc atcaacaata ccttgtctct gatgaaagaa tacttctctg atgaggaaat
    48121 taaaaaatat acagatgtga ttgaaaaatt tgtaccagat cccgaacatt tccgaaagac
    48181 gactgataac ccattcaagg ctctaggtgg aaacttagtt gatatgggaa gggtaaaagt
    48241 aatagctggt ttactgcgta aggatgatca agaaatttct tctaccattc gctcgattga
    48301 gcaagtgttc aagttggtag agcaaggtga aggtttttat caagatggat cctatatcga
    48361 ccacaccaat gttgcctata cgggtgctta tgggaatgtt ttgattgatg gcctgtctca
    48421 actgttgcca gtcattcaaa agaccaagag tccaatcgat aaagataaaa tgcaaaccat
    48481 gtaccactgg attgataaat cgtttgctcc tttgctggtg aatggagagt tgatggatat
    48541 gagtcgtgga cgctcgatca gtcgtgcaaa tagcgagggg cacgtggccg cagtagaagt
    48601 actaagaggg attcaccgaa tagcggatat gtctgaagga gaaaccaaac aacgtttgca
    48661 gagtcttgtg aagaccattg ttcaatcgga tagttattat gatgtcttta agaatttgaa
    48721 gacttataag gatatcagtt tgatgcaatc cttgttaagt gatgcaggag tcgcaagtgt
    48781 tccaagaaca agttacctat ctgcctttaa caagatggat aaaacagcca tgtacaacgc
    48841 agagaaaggg tttggatttg gcttgtcact cttttccagt cgtaccttga attacgaaca
    48901 catgaacaag gaaaataaac atggttggta tacgagtgat gggatgttct atctttacaa
    48961 tggcgatttg agtcactata gcgatggcta ctggccaaca gttaatccat ataagatgcc
    49021 tggtacaaca gagacggatg ctaagagagc ggatagcgat acaggtaaag ttttaccgtc
    49081 tgctttcgtt ggaacgagca aactagatga tgccaatgcg acagcaacca tggatttcac
    49141 caactggaat caaacattga ctgctcataa gagctggttt atgctaaagg ataagatcgc
    49201 ctttttagga agcaatatcc aaaacacttc aacagatact gctgcaacta caattgacca
    49261 gagaaaactg gaatcaagta atccatataa agtctatgtc aatgataaag aagcctccct
    49321 tacagaacaa gaaaaggatt atcctgaaac ccaaagtgtc tttttagaat cgtccgattc
    49381 gaaaaagaat attggttact ttttctttaa gaagagttca atcagtatga gtaaggcttt
    49441 gcaaaaggga gcctggaagg atatcaatga aggacagtca gacaaggaag ttgaaaatga
    49501 atttcttacg attagtcagg ctcataagca aaatggagat tcttatggct atatgcttat
    49561 ccctaacgtg gatcgtgcca ccttcaatca aatgataaaa gagttagaaa gtagcctcat
    49621 cgaaaataac gaaacccttc agtctgttta tgatgctaaa caaggagttt ggggcattgt
    49681 gaaatatgat gattctgtct ctactatttc caaccaattc caagttttga aacgtggagt
    49741 ctatactatt cgaaaagagg gggatgaata taagattgcc tactataatc ctgaaaccca
    49801 ggaatcagct ccagatcagg aagtctttaa aaagctagag caagcagctc agccacaagt
    49861 acagaattca aaagaaaagg aaaaatctga agaggaaaag aaccattcgg atcaaaagaa
    49921 tctccctcag acaggagaag gtcagtcaat cttggcaagt ctagggttct tgctacttgg
    49981 ggcgttttat ctattccgta gaggaaagaa caactaattt gttcatcatc tagatgaatt
    50041 acatgaaact gtggaaaata gaaaaaatcc gaagaaggcc aaatatcttc ggatttttct
    50101 tttaaaaaag ataagttgat acttatcctt ttttacttca ttatgtgact atagcgtttt
    50161 gaaactagaa taggacgtca taactgctaa aagatttcta taaattcatt tgattttcct
    50221 aatcaatttg ttcgtatcct atttcactcc actatattag tccgtctcgc tccgtgaggt
    50281 gcgagacaaa acaaccaccc gctatacggg tgcgcgtcga aggttatacc aaaaaaactc
    50341 caaacgcgat acaataaagg tgttcaatcc aattgtaaag cgaaaggaga aaaaatatgg
    50401 cacaaaagac tcatagttta tcatacacaa agtggatgtg ttctatcata ttgtgttcac
    50461 ccctaagtat agacgaaaag tcatctataa tcaatataga agtagtttag gcgaaatatt
    50521 tcatcgcttg tgtagttata aaggtgttga aattatcgag ggtcacttaa tgccagacca
    50581 tgtacacatg ttagtcagta ttccactaag gctaagtgtt tcaagtttta tggggtattt
    50641 aaaaggcaaa agtgcactca tgatgtttga caaacacgcc aatctcaagt acaagtttgg
    50701 gaatcggcat ttctgggcgg aaggttatta tgtaagtaca gtagggctta ataaagccac
    50761 acttaagaaa tatagtcaag attaaagaaa ttatccagtg gatgatttct tcacgagtat
    50821 gaaaatttga gaactagtaa agtatgatat atagtaaaat gaaataagaa caggacaaaa
    50881 tcgatcagga cagtcaaatc gatttttaac aatgttttag aagtagaggt gtactattcc
    50941 agtttcaatc tactatagca ctagataaat tgagtgtaaa agaatatgag gatcccttta
    51001 gggatagtgg taagtaatac caaagtctct taaagaggca agtgacgagt caagagcaat
    51061 aaggcttgaa caacgtgaaa gccagcgtct ttaggcgctg gctgatgatt tgggcttata
    51121 gctctgagat aaaccacccg ttagacgggt agttatgatt ttatctgagt gtaacatact
    51181 gttgggcaat ctcgctgatg cggtcaaagt tgccttggga agcgagttta ttgagttcgc
    51241 caccaattcc aacggcatct gcaccagcag cgaaccattg agggatgttg tttaggccga
    51301 ctcctccggt taccattacg gaaacttgtg ggatcggtgc cttgactgca gagatatatg
    51361 ctggactgag agtactacct gggaagagtt tgatgatttc actaccggct tcaagtgcag
    51421 tcgtgatctc tgtgagggta atacagcctg gaatgtacgg tgtgctgtag agattgcaca
    51481 ttttcgcagt ttcagcatgg aaagatggag aaacaacgta atttgctcca gctagaatgg
    51541 catctctagc agttacggca tcaagcacag tacctgcacc gatacaaaca ctctgatcgt
    51601 cctgatacaa gtctacaagt tccttgatga tttgtcctgc atactgattg gtataggcga
    51661 tttcaatagc tttgataccg cccttgatac aagcaatcga ggcttgtagt ccttcttcct
    51721 ttgtatttcc tcgaatgaca gcgacaattt tctgtttttt tagttcaata atcgtatctg
    51781 atttggtcat gtaattctcc taacgaatga tatcttgtgc atttgccagt aaattttcaa
    51841 tactagttgc ggaagtggag agatggtctc ctggaagagt gcatttgaga gttgcgctcg
    51901 caactgcaaa gtcaatggta gtttttaggg aggaatgatg gagtagttgg taaagggcac
    51961 cagatataaa ggcatccccg ctaccaattc gttgatagac tgcagttttt agttggacag
    52021 actcttcaaa tcgttcttcc agagcatagg cttgatagac atttttgtct tgctcatcac
    52081 tagatcggag ggtatggaaa atggccttga aaccataggc ttcttttaaa agtcgcatgc
    52141 gattttccac ctcttctagg ctagcactgt ctcttggaaa catctctaga ttttggtcat
    52201 caatcatgag aggatcaata ccgaagcaat agtcagtaaa acgtgcaaac ttagaaaatt
    52261 catacttggc ttctaggact gaaatcatct ttgttctcag attgagatcc attgaaacga
    52321 caattcctcg gcgcttggct tcttccaaga gtaggagaag gatcgcacgg acctcttgac
    52381 cgatagctat ggtgattcca ctaaaatgaa aatggctaat cccctgaaag agagaatcca
    52441 tatctagcat gtttggccga atctggctga tactcgtatg cttacgatcg tagaaaactt
    52501 cactttgacg acaaccaaag ccgttctcca aatagtagag gccgattcga tcgccaagcc
    52561 gacagattga actggtatcg atttggtgct gtttcaagaa tgtgagaaaa cgatctccaa
    52621 tctcgttggc agggagtgcg gtaaagactt tcgttgagat acccagggct tgcaaattac
    52681 aagcgatgtt aatttctgat ccgccaaaat aagtcgaact ggcaacatga tcgccgatac
    52741 tggtggcatc taatggtgag attcgaatta atggttcgcc aaacagtaat attttcgtca
    52801 tccattagtt cctttctggt ttagaatagc tttacttggc taatacttct ttcaaataag
    52861 cagcgacttc atcatcttgg cagttttcaa agaagtattc ttggaagtat ttgccagaga
    52921 tggtgtcttt gaggaagtct tggtcgattg ttttaaggat ggtcatcaaa tcattgtggg
    52981 tgatttgttt cacctcgttt aagatacgag cgttgcgttg ttcagggatt acacgttctc
    53041 ttgggtagcc accgcccatt tcttcagcaa ataagcgttc aaacatcaat ttgagggtca
    53101 gttctgcccc ccagccaaat cctttggcat aagggataga caaggcgtta ccaccattga
    53161 tttgagaata aaggtaagcg tcagttgggt ccactgctag accacagaca acaccaggga
    53221 agctgtttaa agcaagcata gcccctacac ccgtaccaca gccggtaaca acaaagtcaa
    53281 ctgcctttgt atttaaaagg atggcagcca ttagtccgtt ctgcacataa gttaattgac
    53341 tttctccttc ttctccacgc ataccatagt taaataattg gtagtctttt ttatctgtcg
    53401 cttcttttag actatcgtaa ataatgtgat tcttgctagc ttgactattt tcattgatta
    53461 atgcgatttt cataagatct tctttctatt tttactcagg ttgttttccg atgtaggcta
    53521 agataccgcc atctacatat aggatgtggc cattgacaaa attgctggca tcactagcga
    53581 gaaagacagc agggcccatc aaatcttcag gatttcccca acgtgcagca ggtgtttttg
    53641 caatgatgaa ctggtcaaat gggtggcgag aaccatcttc ttgcaactca cgaagaggtg
    53701 ctgtttgagg agtggcaata taacccggtc caattccgtt acattggata ttggctccac
    53761 cgtattcaga cgcaatgttg cgggtcaaca ttttcaagcc ccctttagca gcagcataag
    53821 cgctaactgt ttcacgtccc agttcgctca tcatcgaaca aatattgata atctttccat
    53881 gccctttctt tatcatagaa ggaataactg cctttgaaac gataaatggt gcgtttaagt
    53941 caatatcgat gaccttacgg aaatcagcgg cgctcatttc gcacattgga actcggcgga
    54001 taataccagc gttattaacg aggatgtcaa tgacaccaac ctcttgttcg atttgcttga
    54061 ccatggcttg gataccgtcc tcgtctgtca cgtcacagac atatccatgg gcttcgatgc
    54121 caacttcacg ataagcttca atccctttat tgaccagatc ttgattgata tcgttaaaga
    54181 caatagtagc gccggcttca gcgtaggatt tggcaatagc aaatccaatt ccataagaag
    54241 cgccggtgat gagagcaatt tttcctttta aagaaaactg ctcaattgag aatgatgtat
    54301 ttgtcatttg atacttcctt tcaaatttgt taagacttct ctataatcaa attatattct
    54361 agtaaaccgg ttttgtcaat aaataaatat ttttattta
//
LOCUS       DBEROU010000008        53752 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.008, whole genome shotgun sequence.
ACCESSION   DBEROU010000008 DBEROU010000000
VERSION     DBEROU010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 53752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53752
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.008"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            153..500
                     /locus_tag="ACL8CL_000520"
     CDS             153..500
                     /locus_tag="ACL8CL_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGS1329821.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            complement(779..1615)
                     /locus_tag="ACL8CL_000521"
     CDS             complement(779..1615)
                     /locus_tag="ACL8CL_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1329822.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKSSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(1628..2257)
                     /locus_tag="ACL8CL_000522"
     CDS             complement(1628..2257)
                     /locus_tag="ACL8CL_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1329823.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(2267..2908)
                     /locus_tag="ACL8CL_000523"
     CDS             complement(2267..2908)
                     /locus_tag="ACL8CL_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1329824.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(3054..4283)
                     /locus_tag="ACL8CL_000524"
     CDS             complement(3054..4283)
                     /locus_tag="ACL8CL_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HGS1329825.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSNLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLVL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(4273..5439)
                     /locus_tag="ACL8CL_000525"
     CDS             complement(4273..5439)
                     /locus_tag="ACL8CL_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HGS1329826.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFLAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            5583..6596
                     /locus_tag="ACL8CL_000526"
     CDS             5583..6596
                     /locus_tag="ACL8CL_000526"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HGS1329827.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNNTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(6641..7060)
                     /locus_tag="ACL8CL_000527"
     CDS             complement(6641..7060)
                     /locus_tag="ACL8CL_000527"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HGS1329828.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(7071..8477)
                     /gene="atpD"
                     /locus_tag="ACL8CL_000528"
     CDS             complement(7071..8477)
                     /gene="atpD"
                     /locus_tag="ACL8CL_000528"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HGS1329829.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTKIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(8563..9441)
                     /locus_tag="ACL8CL_000529"
     CDS             complement(8563..9441)
                     /locus_tag="ACL8CL_000529"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HGS1329830.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(9457..10962)
                     /gene="atpA"
                     /locus_tag="ACL8CL_000530"
     CDS             complement(9457..10962)
                     /gene="atpA"
                     /locus_tag="ACL8CL_000530"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HGS1329831.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYAGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(10977..11513)
                     /locus_tag="ACL8CL_000531"
     CDS             complement(10977..11513)
                     /locus_tag="ACL8CL_000531"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HGS1329832.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(11513..12031)
                     /gene="atpF"
                     /locus_tag="ACL8CL_000532"
     CDS             complement(11513..12031)
                     /gene="atpF"
                     /locus_tag="ACL8CL_000532"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HGS1329833.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKNQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKA
                     LIDQYIDQLGEA"
     gene            complement(12045..12761)
                     /gene="atpB"
                     /locus_tag="ACL8CL_000533"
     CDS             complement(12045..12761)
                     /gene="atpB"
                     /locus_tag="ACL8CL_000533"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HGS1329834.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(12796..12996)
                     /locus_tag="ACL8CL_000534"
     CDS             complement(12796..12996)
                     /locus_tag="ACL8CL_000534"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HGS1329835.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(13071..14078)
                     /locus_tag="ACL8CL_000535"
     CDS             complement(13071..14078)
                     /locus_tag="ACL8CL_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGS1329836.1"
                     /translation="MQDNYTTKAKHLTIDSRRLIERWKKEGKSNREIASLLGKAPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLTKELKEKILHYHNQ
                     KFSPEMMVMAKGVNVGISTIYYWIHHGKLGLSKQDLPYPRKGKALKKQASTNFKPAGQ
                     SIEQRPEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGTKKMTPKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSKMEIIFIKRF
                     Q"
     gene            complement(14499..14897)
                     /locus_tag="ACL8CL_000536"
     CDS             complement(14499..14897)
                     /locus_tag="ACL8CL_000536"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1329837.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
     gene            complement(15406..15888)
                     /gene="greA"
                     /locus_tag="ACL8CL_000537"
     CDS             complement(15406..15888)
                     /gene="greA"
                     /locus_tag="ACL8CL_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HGS1329838.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(15959..17614)
                     /gene="mltG"
                     /locus_tag="ACL8CL_000538"
     CDS             complement(15959..17614)
                     /gene="mltG"
                     /locus_tag="ACL8CL_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HGS1329839.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSTEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETPGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(17695..18189)
                     /locus_tag="ACL8CL_000539"
     CDS             complement(17695..18189)
                     /locus_tag="ACL8CL_000539"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1329840.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(18186..18650)
                     /locus_tag="ACL8CL_000540"
     CDS             complement(18186..18650)
                     /locus_tag="ACL8CL_000540"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1329841.1"
                     /translation="MEIPIKIIQASKSDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEATLLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(18660..19994)
                     /gene="murC"
                     /locus_tag="ACL8CL_000541"
     CDS             complement(18660..19994)
                     /gene="murC"
                     /locus_tag="ACL8CL_000541"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HGS1329842.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSITGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(20005..20622)
                     /locus_tag="ACL8CL_000542"
     CDS             complement(20005..20622)
                     /locus_tag="ACL8CL_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGS1329843.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPIAEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTAPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(20670..23768)
                     /locus_tag="ACL8CL_000543"
     CDS             complement(20670..23768)
                     /locus_tag="ACL8CL_000543"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1329844.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLIFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKALPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGPVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVTVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(23861..25027)
                     /locus_tag="ACL8CL_000544"
     CDS             complement(23861..25027)
                     /locus_tag="ACL8CL_000544"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HGS1329845.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IHQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVVDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKC"
     gene            complement(25035..26129)
                     /locus_tag="ACL8CL_000545"
     CDS             complement(25035..26129)
                     /locus_tag="ACL8CL_000545"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HGS1329846.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSVFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGHFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQALEG"
     gene            complement(26287..27009)
                     /locus_tag="ACL8CL_000546"
     CDS             complement(26287..27009)
                     /locus_tag="ACL8CL_000546"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329847.1"
                     /translation="MNFNHVYFGYDENRPVLKDITCSIFKGQKIAFVGPSGSGKSTIV
                     RLLEQFYKPLSGDILMEQSSIYDFNLKEWRSKIAWVSQNNAVLSGSIRDNLCLGLNRL
                     VTDDELMKVLDLVSLGDEIRSMKEGLDTEVGERGRLLSGGRSQRLQIARAYLKDAEIL
                     IFDEATANLDADSEYAIISSLYSVLKEKTVVIIAHRLSTVKDVDCIFFLEEGKITGSG
                     THKELLENHERYARFVQEQMIE"
     gene            complement(27196..27969)
                     /locus_tag="ACL8CL_000547"
                     /pseudo
     CDS             complement(27196..27969)
                     /locus_tag="ACL8CL_000547"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(28141..30099)
                     /locus_tag="ACL8CL_000548"
     CDS             complement(28141..30099)
                     /locus_tag="ACL8CL_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1329848.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRATTNDIVTNLVDGLMENDQYGNYVPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGI
                     KALDDQTVQYTLARPEPYWNSKTTSTILFPVNADFLKSKGDDFGKVDPANILYSGPFL
                     MKAFVSKSVIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKAVGLDVYTKMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(30304..31926)
                     /locus_tag="ACL8CL_000549"
     CDS             complement(30304..31926)
                     /locus_tag="ACL8CL_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGS1329849.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLAMGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKVQADRLRAKFKLS"
     gene            32032..33477
                     /locus_tag="ACL8CL_000550"
     CDS             32032..33477
                     /locus_tag="ACL8CL_000550"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HGS1329850.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVIFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTATYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQAYLVHRVYGLTFDVGVFLNITPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGTTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(33523..33717)
                     /locus_tag="ACL8CL_000551"
     CDS             complement(33523..33717)
                     /locus_tag="ACL8CL_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGS1329851.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTK"
     gene            complement(33818..34466)
                     /locus_tag="ACL8CL_000552"
                     /pseudo
     CDS             complement(33818..34466)
                     /locus_tag="ACL8CL_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000614493.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
     gene            complement(34450..34962)
                     /locus_tag="ACL8CL_000553"
     CDS             complement(34450..34962)
                     /locus_tag="ACL8CL_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HGS1329852.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACRHLGAPYNASFYPDAAGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(35049..35984)
                     /locus_tag="ACL8CL_000554"
     CDS             complement(35049..35984)
                     /locus_tag="ACL8CL_000554"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HGS1329853.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(36055..36354)
                     /locus_tag="ACL8CL_000555"
     CDS             complement(36055..36354)
                     /locus_tag="ACL8CL_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HGS1329854.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(36344..37093)
                     /locus_tag="ACL8CL_000556"
     CDS             complement(36344..37093)
                     /locus_tag="ACL8CL_000556"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016430 - tRNA
                     (adenine-N6)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HGS1329855.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPHSNLNESEHYLLARHEITTNLEEICHSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(37147..37506)
                     /locus_tag="ACL8CL_000557"
     CDS             complement(37147..37506)
                     /locus_tag="ACL8CL_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HGS1329856.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(37508..38908)
                     /locus_tag="ACL8CL_000558"
     CDS             complement(37508..38908)
                     /locus_tag="ACL8CL_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HGS1329857.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVYLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(39203..39442)
                     /gene="rpsR"
                     /locus_tag="ACL8CL_000559"
     CDS             complement(39203..39442)
                     /gene="rpsR"
                     /locus_tag="ACL8CL_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HGS1329858.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(39474..39944)
                     /gene="ssbA"
                     /locus_tag="ACL8CL_000560"
     CDS             complement(39474..39944)
                     /gene="ssbA"
                     /locus_tag="ACL8CL_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HGS1329859.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(39956..40246)
                     /gene="rpsF"
                     /locus_tag="ACL8CL_000561"
     CDS             complement(39956..40246)
                     /gene="rpsF"
                     /locus_tag="ACL8CL_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HGS1329860.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(40399..41745)
                     /gene="asnS"
                     /locus_tag="ACL8CL_000562"
     CDS             complement(40399..41745)
                     /gene="asnS"
                     /locus_tag="ACL8CL_000562"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HGS1329861.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(41761..42126)
                     /locus_tag="ACL8CL_000563"
     CDS             complement(41761..42126)
                     /locus_tag="ACL8CL_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1329862.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKLAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGLEAGGRDNGAPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            complement(42119..43306)
                     /locus_tag="ACL8CL_000564"
     CDS             complement(42119..43306)
                     /locus_tag="ACL8CL_000564"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS1329863.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVTDFTTVILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(43303..43791)
                     /locus_tag="ACL8CL_000565"
     CDS             complement(43303..43791)
                     /locus_tag="ACL8CL_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HGS1329864.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQADQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            44112..44660
                     /locus_tag="ACL8CL_000566"
     CDS             44112..44660
                     /locus_tag="ACL8CL_000566"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGS1329865.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIQ"
     gene            complement(44897..52015)
                     /locus_tag="ACL8CL_000567"
     CDS             complement(44897..52015)
                     /locus_tag="ACL8CL_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGS1329866.1"
                     /translation="MKHSHKKSFDWYSMQQRYSIRKYYFGAASVLLGTALVLGAAASV
                     QTVQAEENKQETTNSISVGRGEAATKPAEVSASNKEKTYAAPTVANPVETTPVKTEEV
                     TKPAEKVEEAKDKKEEVTHQDAVDKSKLLTALSRAKKLESKLYTEASAANLQTSIQAG
                     QSLLGKADATEAELSAAESSIQSFIIGLELRSNSNKETVSETPVAKKADAVESKEGAK
                     PAATTERSAVDSAILPTSTADKVETTSAPASINEILKLGLSLSDARQNPAIRKEDVNR
                     GYSGFRAASNPANPIVSGSGNTVAFADISQGGRSYSFRGYGNSRGGNSIHYDVTTVRS
                     GNSVNFTISYSAPGDSREFVNNNFILDKGDGFGNPSNATITSSNPIVREQSKSISQGA
                     NYVSHSGYSMTSAISTNTEQTIRFSLPIINLNGDLSVRLKPVTFNVDQGGGGAATSND
                     PYSNSNYYYRANPLYLDANPYGGTNNKTVSEDIDFQTVDLPTSKLPEGQTRLVREGEK
                     GQRQITYKVHRFGNETLLGLPISNSVTKEAKPRIMQIGVAKDLIDTVKPRVDQNKVGD
                     TNNLTFYLDNDGNGVYTEGVDELVQKIAIKDGAKGEKGAQGERGLTGAKGEKGDRGER
                     GLTGAQGAKGEKGAQGERGLTGAQGAKGEKGDQGERGLTGAQGAKGEKGAQGERGLTG
                     TQGAKGEKGDRGERGLTGTQGAKGEKGARGERGLTGAQGAKGEKGARGERGLTGAQGA
                     KGEKGAQGERGLTGAQGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKGDQGER
                     GITGAKGEKGAQGERGLTGAKGEKGDQGERGLTGAQGEKGDRGERGLTGAKGEKGAQG
                     QAGRDGVTPTVTVKDNKNDGTHTITINDGRGNVTSTVVRDGFDGASPLVATQRNDADK
                     TTTVIFYYDKNGNNELDASDKKLKEVVIADGAKGEKGDKGEQGLQGRDGEQGPKGEDG
                     KTPTVKVTDGQDGTHTITINDGKGGITTTVVRDGFDGASPLVSTHRNEADKTTTVIFY
                     YDLNDNNQFDEGDTKLKEVVIADGKQGPKGDKGDNGKDGFTPEVTVTDNNNGTHTITI
                     TQPDNRPSLTTIVKNGEDGKTPKVKAERDDAKKQTTLTFYIDKDGDGSYTAGKDELVQ
                     TTVVKDGQDGAAGASGRDGKEVLNGKVDPTTEGKDGDTFVNTQTGDVFVKKGNTWEPA
                     GNIKGPKGDKGADGAKGEKGAQGERGLTGAQGVKGEKGDQGERGLTGSKGEKGAQGER
                     GLTGAQGAKGDKGEQGLQGRDGAQGPKGADGQRGPAGPQGPKGEQGNPGTPGKDGKSL
                     IAVKNGTETKVYVEDPARPGQPLNPNQPLATITDGRNGTNGQSPTITATRSVQNGKNG
                     VLVTITPVGGRPQTTFVEDGQKGADGKTPTVTITEGQNGTHTLTVHNPGSPDVTTTIR
                     DGATGQAGRDGKDVLNGKVNPQPNQGKNGDKYINTETGDVYVKNNGNWDKEGNIKGPK
                     GDKGADGAKGEKGDQGERGLTGAQGAKGADGAVGRDGRDGKDVLNGKANPEAHQGKDG
                     DKYVNTETGDVFVKNNGNWDKEGNIKGPKGDKGERGEDGKTPEVTVTPGKDGHSTDIT
                     FTVPGKDPVTVNVKDGENGLNGKTPKVDLLRVQGKNGNPSHTIVTFYTDENNDGKYTP
                     GTDELLGSEMIKDGAKGADGRDGKSLLTVKDGKETKVYQEDPANPGQPLNPEKPLAVI
                     RDGVDGKSPTVTAVRKDEAGHKGVEITVDNHDGSQPTTVFVQDGAKGETGATGQDGQT
                     PTITTQRGQDGQSTVVTITTSGKDPVTFTVKDGKNGKDGRAPKIKVEDITSPSRIRRD
                     TDAAATPTRNGIRVTVYDDVNDNGVYDEGVDKVLNSKDIYNGIDGRDGSAPTITTKDN
                     GDGTHTITVQNPDGSESTTVVKDGKDGKTANITTTENPDGSHTITVTNPDGSTKETVV
                     KNGKDGKTPKVEVTDNNDGTHTVKVTDGDGNVTNAIIKDGKDGKAATATTTENPDGSH
                     TVTITNPDGTKNEFVVKNGRDGVDGRTPTASVRDNGDGSHTIVITNPEGVTTETTVRD
                     GKSPKVTITDEQNGTHKISVLNGDGTTTTTTVRDGREPKLEVIDNNDGSHTIKVTGAD
                     GKGTTTTIFDGKSPKANIVDNGDGTHTLTIVDSDGREYKSIIKDGKDGKDSVSPTVTV
                     KNNNDGTHVVTITNPDGSKTEMVIKDGKDGKSPKVSVEDNGDGSHTITIINSDGTVTK
                     TVIKDGRDGRDGRDGKDGKDGKDGKCGCQDKPVTPSNDKPVPPTPNVPTPEVPVKPVP
                     AVPEQPVVPTPAQPATPVNANPVAPTTGKENRGDKLPETGSQSDYISVLLGSGILLSL
                     YVGRRKEA"
     gene            52231..53715
                     /locus_tag="ACL8CL_000568"
     CDS             52231..53715
                     /locus_tag="ACL8CL_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003096885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator PRD
                     domain-containing protein"
                     /protein_id="HGS1329867.1"
                     /translation="MRNLLSTKVQRQLRLMETLIQNRNWMKLHELAEKLGCTERILKS
                     DLNELRIAFPSINIQSSVNGIMIDLEVNTSVEDIYQYFLANSQSFQLLEYMFFNEGLP
                     IYRTIENLYFSSANLYRLGRNITKVLSSQFQIELSFTPSEIRGNEIDIRYFFAQYFSE
                     RYYFLDWPFPDLPEEDLTEFADFFYKITNYPMRFSIYRMYKLMIAISIHRVKNGHFID
                     LPNHFYKEYYPLLKSIPNFQETLAYFSKHFGLEMTPDTIAQIFISFLQNDIFLDPQEF
                     FNSLEDNSQARYSYQLLSQILEGLSKQYKITFTNHDELIWHLHNTAFFERQEIFSTPI
                     LFEQKALTIKKFEVYFPDFMGSARQELAQYRQAIGQHDHPEQLEHLMYTILTHAENLS
                     TQLLENRPPIKVLIISNFDHAISLTFVDMLSYYCNNRFTFDIWDELKTSPEILNQTDY
                     DIIVSNFYIPGITKKFICRNHLSIMNLVNHLNTLSNEIHLSNTL"
BASE COUNT        15263 a        12013 c         9772 g        16704 t
ORIGIN      
        1 gctccataat atctataagg gatttaccca ctacaaatat tatagagcct aaaagatgag
       61 accaaccaac ctcatctttt ttcgtatcaa atctaagcaa ttttagaaac tttatggcat
      121 actagactta tcaatgaaag cgaggtaatc tcatggaaca atttttagat aacatcaaag
      181 accttgaagt cactacagtt gtgcgtgcgc aagaagctct tgataaaaaa gaaactgcaa
      241 ccttctttat cggtcgcaaa acttgccctt actgccgtaa atttgcaggt acattgtcag
      301 gtgtcgtagc tgaaaccaaa gctcacattt acttcatcaa tagtgaagaa ccaagccaac
      361 tcaatgattt gcaagcattc cgctcacgct atggaatccc aactgtacca ggttttattc
      421 atattacaga tggacaaatc aacgtccgtt gcgactcttc aatgtcagca caagaaatca
      481 aagatttcgc aggattgtaa aaaatcagct aacaagtctt aaaatcacaa aaacgcataa
      541 tgtaaggtgc aaaaaccttg atattatgcg ttttattgtt cctaaaacag ttacatcagc
      601 atttcattac agaacatttt aattttggaa gtcaaatcaa tttttaacaa tattttagaa
      661 atcacagtgg gctattccat attcaattat ccagattcaa tcgctattat cgttttccta
      721 gattacaaaa aaaactacgt tttgctaatc aaaacgtagt ctcagccact attttatctt
      781 actgtccttc ttttacttct ttggttgcta catcttctcc aaaccatttt tggctgattt
      841 cttggaactt gccgtccttg taaagactag aaaaagcttc atttatcttc ttaaccaagg
      901 ttgtatcttc cttacgggct ccaaccgcaa aagcttctgt ttctagtcca actgtaaaga
      961 cattataatc gtttaaaaca ccttctgctt ctaaataata gtttgcatag acacggtcaa
     1021 tcaatagacc atcaattcga tcgtttttca aatcaatcaa ggcttcatta aaggtttggt
     1081 attgattcgc ttccttatta gcgacaatat tcttcaaaag ttctggattt gcttcaaagt
     1141 ccgcataacc agatgaacca gcttgagctc ctaatgtctt tccagtcata tcctttgcag
     1201 tcgtgatacc agatgatttc ttcgtaacca atacctgctc attcttcata tatgagttac
     1261 tgaaagccac cttttcacgg cgttcgtctg tagcggaata gccattccaa atcagatcaa
     1321 tcgttccttt tgtcaattca gcttctttca aatcccaatc aatcggttgc caatttaccg
     1381 tgattccgta tttttcaaaa acagctgtag ctaaatcaat atcaaatcct gcataagaac
     1441 catctttctg agcaaatccc attggaacaa aagtactatc aaatccaata gtaatagact
     1501 tgttagactg gtactttgac caattatctc cactagtttc gctagaattt ttcccacaag
     1561 ctactaagaa caaagcagtc atcagactga ctaatacaag catccatttt ttcatccatt
     1621 tttcctccta tttaggttct actttcaata atacatctgc gatattttca gcaaactgca
     1681 aatcatgggt aaccacaatc tgggtcatcc caagttccct attttgcaag attagcttct
     1741 ccacttccaa acgtaattct ggatccaggg cagaagttgg ttcatcgtag ccaatgattt
     1801 ctgggtcaat catcatagca cgcgccaaag ccactcgctg cttttgccca ccagatagtg
     1861 agaaaggata ggcctctgcg tgtcctccta gtcctaactg ttccaagagt ccactcgcct
     1921 tcttctcagc ctcttcctgc ttcattccca tggtcttcac aggcgataaa gtcaaatttt
     1981 ccagaactga tagatgagga aatagttgaa aatcttggaa gacaaatccc agtaggttgc
     2041 gcttctgcaa ttcatccagc tctaaaggtt gtccattata aaagatttgc cctgaatcaa
     2101 tggtttcaag acctgcaagc atacgtaaaa gagttgtctt acctccacca gaaggtccaa
     2161 cgatagccag gatttgcttt tcaggaatac ttagactgaa attagacagg atttgtttgt
     2221 ctccaaagac tttattgata tttcgtaatt ctaacatggc agcctcctat ctataataac
     2281 tatacttctt ctcaactttt ttggaaataa ttgtcacaat cccaatcaaa atcaaataaa
     2341 tggctcctgc caagaacata ggaactagac tagcatcgcg gttagcagct gttcgactag
     2401 ccaagataag gtctgaaatt ccgagagcat agaccaaaga agtatccttg accaaactca
     2461 taacttcatt aaagacacta ggaagaacaa tcttggtcac ttggggcaag ataatatagc
     2521 gcactctgtc aaaagggcta aactttaaga ccttggcagc ctcatactgt cctcttggaa
     2581 tagtgtcaat tcccccacgg aaaatttctg caaagtaagc tgcatagttg agaacaaagg
     2641 caataatagc tgcaggaagg cggtctaaac gaatcccaat acttgggagc acataataga
     2701 taaaaatcag ttgcaagagt aagggggttc ctcgcataac ccagatgtaa atgttaatca
     2761 gataatggag gggcttccaa tggacttgca aggcaaaggc aatcaaaacg cccaagggaa
     2821 tcgaaaatag caagaccagt gcaaagacct gtacagtcgt gctcgcacca ttcagtaaac
     2881 tcggtaatat ctcaaataaa taagacatac tgcacctcct aaaagtaatt gttcctatta
     2941 tagcatgaat aaacaagatt accaagtatt tttgtaaaaa gattctacca tatacaaata
     3001 aaaaccgtag ctatcaaaag agataaacta cagttctata tgtttaaaca ttcttacaat
     3061 ctctcaaaca gttcttgcat ttgcacatca tctggaacca gttttaagta agtgtgagca
     3121 tggacttttg cttcttcaaa atgtcccaat tcacgcaaga gatagatata gtgttccaga
     3181 aattctggat tgtccttcaa atctcctgtc aactcttgat aatgctcata agcagtatcc
     3241 aaatcgtcca tttcttgata agaacgagca atcatccact tggtcaaaag attttctggc
     3301 tcctcactct gcaagtctag aatatcctca taacgctcct gctccagata aatagtggct
     3361 aaacgaagca agatttcttc tgtatcctca gcgtcttctt ttgcagtaag gagataattt
     3421 tctgcaccac tagcatcatg caattcataa gaaaattgtg aagcagctag caagaggcga
     3481 gtttcaaagg gatttttctc taatccttgc ttagcgatac gcagggcttc ttgaacttga
     3541 tgttccttat gtaaagcctg actgtaccca tactcatagc cttcaaagtc aggagaaatg
     3601 gtatcaagct gcttaaagta gagggtggct ttttgatatt cttcttgatc aaaataaaga
     3661 ctggccaact caaaagctgt taagtcatcg tattctaact ccagggcttt ttctaaaaac
     3721 tcagtagccg tttcaaattt ccctaactga gcataggcaa agccaattcg ttgataggtg
     3781 gaaatgcccg tttgctcata aatcgagcga ttatctaact gggcataggc ttgaatagcc
     3841 gcttggtaat tttccaactc actatccaac tctgccaaac ccaatatcaa gagagaatcc
     3901 tctgagtagg tcaaggcctc caataatttc tcacgtgcca catctgtcaa accttccagc
     3961 tggtaaaggt ctgccttcaa aaccaaagac gagacatacc agtcactgtc agcttggatt
     4021 tcctcaagat aggtaaaggc ttcttctatt tgaccatcct cgctagcaat tgcagctaga
     4081 ttaagatgaa cctctggaaa atcctctaca attttcaggt aaatttcctt ggcctgagga
     4141 tagaaaccaa tcccttcaag ataagttgcc aactcataca gaagattact tgaatcattt
     4201 tctaaagctt tggcgaaata atgctcagcc ttagttaaat cttgctcctc caaagcctgt
     4261 aacatctgtt gactattgtt cactcttgac ttcctcaccc tctaattcat atagaccact
     4321 gactacctta taccattcga aaatggctga aatgacaacc ttagcagagg cataaaccgg
     4381 aataccaagt aaaactcccc agataccaaa catagatcct gaagttaaca aaacaaagag
     4441 aacattaata gggtggatgt ttaattgact tcccaaaatc aatggagaga caaaacggcc
     4501 ttcaatagtt tgttctacga taaagacaat cactactttc aaaagcatga ctggaccagc
     4561 aatcaaaccc aatactagag caggaagcat ggctagaaag ctaccaagat aagggaccag
     4621 atttaaaata ccagcagtaa cccccagcgt aaccgcatag cgtagaccaa taatcttgaa
     4681 gaagatgata aacattactg ctacaataat agccactgtc acttgccctc gaacatagtt
     4741 ggacaactgt tgattcacat ctgataaaac ttgtccaaca ggttccttca attttcttgg
     4801 aatgaattgg gtcaaatagt tacgcaagcc tttcccatca cgcaagagat aaaagagcat
     4861 gaaaggaacg ataatcaagg caacaatcac ttgagaagcc ccgctaataa aggcactcac
     4921 ccagttgact gcctgagatg aaaccttact tgccaaaact gtagcctggc tagaaaaatt
     4981 ggtcaaaact tgctctaatt gaggtctgaa atcatctggc aggtgctggg ctaccaatcc
     5041 attaacaatc ctatctatat cttctaagta aacaggaacg tttcttgcaa aggtcaaaac
     5101 ctgacgttgc agatttggaa tggcgactgc caagccccaa atgataaaga gagcgatgat
     5161 aacaaagaca atagtgatag ctataacacg attaacctta tgcttctcca tccaatcaac
     5221 aataggattc aacaaataat ataacaaacc agacaaaatg actggcaaca tcacaactgc
     5281 taaaaagtct aaaacaggtg aaaatagaaa actaatctta cttaaaataa aaagattcag
     5341 tcccaataat aaggttacta aaaataccgt aattgccttg ttatctaaaa accacttgaa
     5401 aaaccaagat aggctaaaat gtttctcttt ttgctccata aatacttcct ttctattgtt
     5461 ctctcattat accatttttg aaacaaaata actctttttt aaagtgctta catctaattg
     5521 gatatcttta atttacttgt ttttgtgata taatagtctt atctttacac agaggtatag
     5581 taatgaaaga aactgtttat tttggaactt atacacgtcg tacttctcaa gggatttaca
     5641 aggcagactt tgatacagaa actggtcagc tttcaaatct agaacttttt gcagctgagc
     5701 caagtccaac ctaccttgcc tttgaccagc accaacattt atacactgtt ggtagccaag
     5761 acgataaggg gggaattgca gcctatcaaa ctgacgggac tgtgttaaat catgttgttg
     5821 aagaaggagc tccccactgt tatgttgctg tcgatgaaaa gcgtgatttg gtttacgcag
     5881 ctaactatca caagggacaa gtccttgttt ataaacgcca ggaagatggt agtcttctac
     5941 ttagtgatat ggatcaacac agtggccaag gtccacatga aaatcaagct tccccccatg
     6001 ttcactatac agatttaaca cctgaccact atctagtgac ctgcgacttg ggaactgacc
     6061 aagtcatcac ctatgacctc gatcaagaag gaaaattatc taagctctat acctatcaca
     6121 gcaagccagg agcaggctca cgccatatca ttttccataa ccactataaa atcgcttatc
     6181 tcatttgtga actcaataat actatcgaag ttttaatcta cgatggcgtt ggcgaatttg
     6241 aacgtatgca ggtcatttca actttaccgg aagcttacga aggctttaat ggaaccgctg
     6301 ctattcatct ctctaaagac ggtaaatacc tctacgcttc taaccgtggc catgattcta
     6361 tcgcagtata taccatcctt gcggacggta gcttagagtt attagaaatc gttccaacgc
     6421 atggtcagac tccacgtgat tttgatttga cacccgacca aaaatttctc attgttgtcc
     6481 atcaagactc tgacaatgca actgtcttta aacgtaattg tgacaatggt cgtctagcag
     6541 aactctccaa cgacttccat gttcccgaag cagtctgcat ccgttttgct ccttaaaaca
     6601 ccataaaaaa tgaacttggt attttcaagt tcattttttc ttatagtcta tttccgacat
     6661 taatacggtt aatggcacgt tgcaaagcaa tcttagcacg acgttcttgg tcaatcaaat
     6721 gtttgtcttg tgcttcttca attgcacgtt ctgcacgaag tttggcacgt tctgcacgac
     6781 tgatatcgat atcacgagca cgttctgcag agtcagcgac gattgtgatc atatcattgg
     6841 caatttcaat aacgccaccg tttactgcaa tccagttcac gtgatcttta tcatcgatac
     6901 gttttacctt tacttcatca actgctaaaa ccgcaatcat attttcatgt cgtggcaaga
     6961 tccccatctc accatccaga gttcgaaccg atacatagct ggcatggtga tcatagacga
     7021 gaccatctgg tgtcacgatc tggacagtta actgagccat agatcacctc ttaaaatccc
     7081 attttttcag cttttgcaat cacatcttcg atagaaccta caccacggaa ggcatcttct
     7141 ggcaagtggt cgtatttacc atcaaggatt tccttaaagc cacgtacagt ttcagcaact
     7201 ggaacataag aacctggctg accagtaaat tgttccgcaa cgttgaagtt ttgtgacaag
     7261 aagaactgga tacgacgggc gcgagcaacc aaggtctttt cttcatcaga aagctcatcc
     7321 ataccaagga tagcaatgat atcttgcaat tcatggtaac gttgaaggac acgttttact
     7381 tcagcagcaa ctgcatagtg ctcttctcca acgatttcag gtgccaaggc acgtgagctt
     7441 gaagcaagtg ggtcaacggc tgggtagata cccaattgta ccaacttacg ttccaagttt
     7501 gttgttgaat ccaagtgagc gaaggctgtt gctggcgctg ggtcagtata gtcatccgct
     7561 ggcacataga tagcctggat agaggttaca gaacccttct tggttgatgt gatacgttct
     7621 tgcaattgac ccatttccgt agcaagtgtt ggttggtaac caacggctga tggcatacga
     7681 cccaaaaggg cagatacttc tgaaccagcc tgagtgaaac ggaagatatt atcgataaag
     7741 agaagcacgt cttggccttc cacatcacgg aagtattcag cgattgtcaa accagtaagg
     7801 gcaacacgca tacgtgctcc tggtggctca ttcatctgac caaagaccat ggctgttttc
     7861 tcgataacgc ctgattcttt catttcccag taaaggtcat tcccctcacg agtacgttcc
     7921 ccaacaccag taaatactga aataccaccg tgctcttggg caatgttgtg aatcaattct
     7981 tggattaaga cggttttacc aactccggca ccaccgaaaa gtccaacttt accaccttta
     8041 aggtaagggg caagaaggtc aataaccttg atccctgttt caaggatttc agaagaggta
     8101 gacaactcat caaaagttgg agctttttta tgaattggct gacgctctgc gtcttctgta
     8161 aaaggagctt ccaaatcaat ggtatctccc aaaacgttga agacacgtcc caaagtttct
     8221 ttacctactg gtacagagat tggacgacct gtgtccaata cttccattcc acgagtcaac
     8281 ccatctgttg attccatggc gatagtacga accataccat ctcctaactc caaggctact
     8341 tcaaggacga tttttgtttt tctttcgtca tttttgtaga cgacaagtgc attgttaatc
     8401 tcaggaagtt tttcccctgc tgcaaacaaa acgtctacaa cgggaccgat aacctgagca
     8461 attttacctg aactcatctc cttctcctat tctatataag atactgtcgg ttcctagctc
     8521 aactaggtcc taagttcatt ttcatacgag ctggactaga gcctattcta aggcactagc
     8581 acctgctacg atttctgtaa tttcttgtgt aatcgccgcc tgtctggcac ggttatactg
     8641 aattgtcaaa tcattgatga ctttcttagc attatctgtc gctgtttgca tggctgtcat
     8701 acccgcagca ttctcagctg tcttggcatc gataatggca ccgtaaatca tactttctgc
     8761 aaactgaggc aacaactgct ccagaatttc ttctcggctg gtttccaatt caaaagtcaa
     8821 gctgtactct tcatccgctt catttggatc caagtcaaca atcggaagca tttgttccac
     8881 acgcatttga ctggttagcg tattgacatg gtggttgtag caaacataaa gctcatcaaa
     8941 gagttcattt tggtacattt caacagtttt tgaaataatc ttacgaactt gatcaaagct
     9001 aggttggtct gccaagccac gtaattcata aagtggttga ataccgcgag ccttaaagaa
     9061 atcagctccc atcccaccga tacagatcat ttcaaaacct ttaccgtctg ggtggtattc
     9121 ttctttcaac tccataacag ctttcaaaat agaggaatta taacctccaa ccaaaccgcg
     9181 gtctgaagtg ataacgatat agcctgtctt cttcacagaa cggctaatca acatcggatt
     9241 agttgaagca ccagctccat taccatgaag gatatctgtc aaaagtttac gcactttctg
     9301 agcgtaaact tggaagttgc gagcagcttc ttcagaacga cctagcttag cagccgatac
     9361 catttgcatg gcattagtga tttgactcgt attttttgtt gaggcgattt ttgttttaat
     9421 atcatttaga gatactgcca tctgacacct ctattcttat tggaagctag attgattgag
     9481 aaactctgta atcgcagcat ccaagactgc ttcttctggc aagtcttttg tatcacgaat
     9541 ggtttccaaa atctctggat gttgagcatc aaagaaggta tggaactctt cctcgaaacg
     9601 aacaatatca tctactggaa cagtatccaa gaaaccatgt gtcaaagcat aaagaatggt
     9661 tacttgtttc tcaacaggta atggtttgtg aacaggttgt ttcaagacct caacggtacg
     9721 acgtccacgg ttcaacttag cctgtgttgc tgcgtccaag tcagaaccaa acttagtaaa
     9781 ggcttccaac tcacggtatg aagcaaggtc gatacgaagt gtaccagcaa ccttcttcat
     9841 ggctttgatt tgtgcagaac cacctacacg agatacagat gaacccgcat cgatggctgg
     9901 acgaatacct gcattgaaga ggccatcgcc aaggaagatt tgtccatcag tgatagaaat
     9961 cacgttggtt gcgatatagg ctgagatatc tcctgcttgt gtctcgataa atggtagggc
    10021 tgtaattgat ccaccaccaa gttcatcaga aactttagct gagcgctcaa gcaaacggct
    10081 gtggagatag aaaacatccc ctgggaaggc ttcacgacct ggaggacgac gaagcaagag
    10141 cgacagttca cgataagcta ccgcttgttt tgatagatca tcgtatacaa tcaaaacatg
    10201 cttaccttga tacataaatt cttccgccat agcaacccca gcataaggag ctaggaagag
    10261 caatggagat ggttgtgaag cagaggctgt cacaacgatt gtgtagtcca aggcaccgta
    10321 ctgacgaagt gtttctactt gcgtacgaac tgttgattct ttttgtccaa tcgcgacgta
    10381 gatacagatc atatcttgat ctttttggtt caagattgta tcaatcgcaa tggttgtttt
    10441 ccctgtctga cggtcaccga taatcaactc acgttgacca cgaccaatcg gtacaagggc
    10501 gtcaatagct ttcaaaccag tttgcaatgg ttctgaaaca gacttacgtt gcataacacc
    10561 aggagctggt gcttctactg gacgagtttt atcagtgtgg atttctccaa gaccgtcaac
    10621 tggacgacca agcggatcca caacacgacc aatcagactt tcacctacag ggacttccat
    10681 gattttccct gtacggcgga ttgtatcgcc ttcacggata tctgtaaagt cacctaggat
    10741 gataatacca acgtctgttg actccaagtt ttgagccata ccataagagc cgttttcaaa
    10801 attcaacaac tctccactca tgacattttc aaggccgtga gcacgcgcga taccgtcccc
    10861 gatataggtt acaacacctg tttcagtcac atcaaaattg ggtttgaaat tttcaatttg
    10921 ttgcttaatt aaagcgctga tttcttgtgc gttaattgcc aaaagaacac cactttctat
    10981 ttcaaatttt ctttaacaac tttaagttgt tgtttaatac tcacatcaat tgtcttgtga
    11041 ttggcaaaaa tgacaaaacc accaatgaga ctttcatcga tttgttcttt tacactcctt
    11101 actttcagag acattttttt ctcaatcaaa gggagcaaac gagtcttctg ttcatcagtt
    11161 agaggatgag cagacgtaat cgtcacttca aatcgatttg tttctttttc aagtcggttc
    11221 aagcaatcta caagcacatc ataaaaaaga tttgctctat gattgtaggc cagaacctgg
    11281 ataaagtttt gtaataaagg tgacacagaa tcttggaaaa aagcaattgt tttttcctta
    11341 tccgactcgt ctactgccac ttgctttaaa aaagaaggca gacctgtttt ttcaacaact
    11401 tgcttgattt gagtcaagtc tgaaaagata cggtcttctt ctcctttttc aagtaccaat
    11461 tggacaaaag gcatgctata tttttcaatt acctttactg ttttcttgtc cattaagctt
    11521 ctcctagctg atcgatatac tgatcaatga gtgctttatg ggcatgactg tcaaggtttt
    11581 gtgagatgat tttaccagct aagctgatgg tcaaatctgc gacctcaccc ttaacactct
    11641 gtaaagcttc tactttattt tgagcaattt cttgattttt attttgagca atttcttgat
    11701 tggctttttc ttttaagtgt cctgcttcta gtttagcatc tgctaagata ttagccttac
    11761 tttgctcagc tgtttccttt gcattttcaa tgattgtctt agcttcttta cggctaccag
    11821 ccaattcatc ttcgcgtttt tgagccaata cttctgcttt ttgacgggct tcttcagctc
    11881 tgtcaatatc tgaagcaatt ttttcagctc tttcttcgaa aatgcctgta atattagacc
    11941 atgcaaattt tttaatcaag actagcaaaa gaataaaaga gccagtgatt aaaataaaat
    12001 taccaattaa ttcacctact gttacgtgca tcagttactc ctttctattc ctcttcaata
    12061 ttaatcttat tccctaaata cacagatgtc aaaagagtaa aaacataagc ttggatgcag
    12121 gaaataaaga cagaaaatgc agtccaagcc aaattagctc caaaggctac tggataccaa
    12181 taaatagctt ggtgggaaag aagaagtaac aaactcgtca tgacctctcc tgcaaagata
    12241 ttcccaaaaa tccgcaaagc caaagataag aagtttgtaa attcttccaa gatattcatc
    12301 ggtatgacaa aaacaggact cataaaagat tttatacttt ttttaaatcc acgacgacga
    12361 acgctttcta tatgtgtcaa caaaatgaca agaaaagata aggttaagtc atactgtaaa
    12421 ttagcggttg gcgaactcca ccagttagtc ccatcgatcg tttgaagctt tgtcattaag
    12481 ccaaggttat tggcaatcac catgaaaagg aataaacaaa ggaaaaagag tgagtaatct
    12541 ttcatgtagc gcgaaccaat gttaggttct gtaaatccaa taacaaagtc atagacatac
    12601 tcaagtacat tttgctttcc tttgggtttc aaggtcatat tgcggcttgc ccaataaata
    12661 aagacaaaaa taactcccac aatcaacaaa gtcatggcta acatagtcag attgaagata
    12721 acaggaccaa tagagatgat tggattaata ctttcttcca tctaaaatcc tcccttttct
    12781 tctcgttcca tgtatttatt tgataataaa tgagaagaca agagttacaa agaaagttcc
    12841 ttcaataaag gcaacaccta aaaacatcaa actacgaaac tcagaaagca tatctggttg
    12901 gcgtgctact gatttaaaca gtccattcat caataaacct tcaccgacag atacgcccat
    12961 acaggcaata cataagccta aaaatgttaa attcataaca aattctcctt taaaataaaa
    13021 tttacttact tagtgtagtc ctaaaattta aatttgtcaa cttttaaccg ctattggaag
    13081 cgtttaataa aaattatttc cattttgcta aatttcaagt tcaagttagc catccagaag
    13141 tcttctctgg gtgacttgta gtccaagcat tttttaggat agttgttaat ccacttttcg
    13201 atgaatgcga cttctttggg agtcattttc ttggttccct taggtaacca tctacgaatg
    13261 agcctgttat gattctcatt agttcccctt tcccaagagg cataggggtg cgcataatag
    13321 atgtgctcct cagaaaatac atcaaacaag cgattgaatt ccgttccatt atctgccgtg
    13381 atggaaagaa tcttgtgttg ttttaagatg agttttagag cctgattgac cacctcagca
    13441 cttttatttg gaatcaatcg gatgatctga tgtctgctct ttcgatccgt caagacaagc
    13501 aagcagtagt ttttcgctct cgtaagtaga accgtatcaa tctcataatg cccattctcc
    13561 aagcgaagat tgatagcttc aggccgctgt tcgatggatt gaccagcagg tttaaagttg
    13621 gtgctagcct gtttcttaag cgcttttcct tttctagggt aaggcaaatc ctgcttgctt
    13681 aaccccaatt ttccatgatg aatccaatag taaatggttg aaattcccac gttaacccct
    13741 ttagccatca ccatcatttc aggcgaaaat ttttggttat gatagtggag aatcttttcc
    13801 tttagttcct tggtcaagct tgatttcttg accgagcgct tgcgattgtt ttcataagac
    13861 tgttgagcat agtcggcaga ataaacttct ttgaagcgcc cttttccaag acattgtcgg
    13921 actgtcccgc gcttgatttc agtgtggata gtttgaggag cttttccaag tagagaggca
    13981 atttctctat ttgatttccc ttcttttttc catctttcga ttaagcgacg gctatcgatt
    14041 gtcaaatgtt tggcttttgt agtataattg tcttgcatct ctgtgccttt cttgtgtttg
    14101 tggttgaaca acaagtataa tacagaggtg ttttcttatg cctacaagag ctttcattat
    14161 ttccattttc ttttggattt cactctattc tgaaaaactt gtgtatattt actgaaacta
    14221 gcaagtctta cctgtaaatt taatgaaagc aacacaaaat ccgagagggg aatctcggat
    14281 taatgtcaat ttaaaaagat agagagtttt tagtttaaat aaattgtttt aaaatatcaa
    14341 caacatcact tcttttctta acctgataag ccttaattcc taattttggg gctacgatta
    14401 tattgtcctc aatatcgtct agaaagacac aatttctagg ttataactgg tatttatcga
    14461 tagttactca tatatatcag tccacttcca tactcacctt acagattctt ttggtaataa
    14521 tatctttgcc taatgtagag ataatcttgc aaagaaaaaa cttccttgta gccatgtttc
    14581 tgataaaagt ccggtgcctg gaactggtaa gtattgacaa aggcaaaaca acaatttcga
    14641 ttcttagctt cactttctgc ctgttgcaat agttttgaac cgattccttg ccctcgcagt
    14701 tcctctttta caaacaaata ctcgatttct agccaatttc caaaagtctc tgctatcaaa
    14761 cctgccagga gattgccctt ttcatcttcg acataaagat taagtggctc actttcagcc
    14821 tcttctcttt ttgaacggtt ataaacacga atcagattcc ctatttcttg cgatttatgt
    14881 gattccttat tttccaatct aaagtatagt gaaatgaaat aaaacatgcg caaatcgatt
    14941 aaggaattta atctaatttc taacaatgtc ttagaaatca aagtgtacta tttttaactt
    15001 caatgcacta tacatctaat actcaataaa aatcaaagag caaactagga aactagccgc
    15061 aggttgctca aaacactgtt ttgaggttgt agatagaact gacgaagtca gctcaaagca
    15121 tggttttgag gttgtagatg aaactgacga agtcagctca aagcatggtt ttgaggttgt
    15181 agatgaaact gacgaagtcg gctcaaaaca tggttttgag gttgtagatg aaactgacga
    15241 agttagctca aaacactgtt ttgaggttgt agatgaaact gacgaagtca gtaaccatac
    15301 atacggcaag gcgacgctga cgtggtttga agagattttc gaagaatagc aaaatggaaa
    15361 aaggagtgag tgaagcacat cgcctcccca ctcctttttc tgtttttagg ctgttttttc
    15421 aaccttcaag atttttacat catagctacc aacaggcgtt tcaatggttg ctgtgtcacc
    15481 tgttttcttg ccaatcaagg cctgcccaat tgggctttca tttgaaacct tacctgcaaa
    15541 ggcatccgca ccagctgaac ctacgataat ataaacttct tcttcgtcct caccaatttc
    15601 ttggatggtg actgttttac caatcgctac ttcgtcctgg gcaactgcgt cgctattgac
    15661 gatttcagca tagcggattt ttgtttctaa gctagagatt tgtccttcga caaaggcttg
    15721 ttcatcctta gctgcttcgt actcactgtt ttctgaaaga tcaccgtatg aacgggcaat
    15781 cttaatgcgt tctaccactt ctggtcgacg aaccaatttc aattcttcta attctttttc
    15841 aagtttctcc ttttcctcaa gggtcatagg atatgttttt tctgccattt ttctcaactt
    15901 tcttctgata atattttcta aagaaaatta tgtgaagtat cacataattt tagtttgttt
    15961 agtttaattt gctgttgaca tgttcagcga cattgcggtc gtggtcttct tgattgttag
    16021 catagtagac cttgccttct gtgacatctg ctacaaagta gaggttatcg ctcttagttt
    16081 gattgatgct tgactcaatc gcatccagac ttggactatc gactggacca ggcatgagac
    16141 ctacattttt ataaacatta taaggtgaat caatgttggt atcaatcgca acatcctcag
    16201 ctagactgat attttgcccc agttttcctt gggcatacaa gattgcaata ttactttgaa
    16261 gtggcatatc acgattcaaa cgattgtaaa atacacctgc aatgagctta cgatcttctg
    16321 tcttggcacc ttctttttcg accaaggaag caatggtcaa caactcattg acagtcaagt
    16381 ttttagattt gatagtacta tagtaaggag ataggttctt atccatagca gctaacatct
    16441 catcaatcaa gctctcaata gttgtgcttt ccttgataga gtatgtagct gggaaaaggt
    16501 atccttccaa acgataacgc gcaccgctgt cttttacagg caaactttcc agtaaagtag
    16561 gatatttcgc tactgcttga ctgataaacg tctcatcttg aactttagcc aagaaagcct
    16621 ccgctgtcaa agactctttg aagtcacctt gcaattgacc cacagtttga gcaatctgat
    16681 ccaaggtata accttctgga attgtcaaag tcgcaagtac aggttcttgc ggttcatctg
    16741 ttccaccttt ttgcaactct ttgagtaagt cttctgtact catactcttt tgcaaattgt
    16801 agtaacctgc tttcaagtcg gtataatttt tatacttggc ataaaaacta aaaatcagac
    16861 catgctttac caaaccagct ttttcaagcg tcgtaccgat ttcttgaacg tttgaacctt
    16921 ctggaattcc aaccgtcaca tatttcttag aattagcatc gataggtaat aaggaatcta
    16981 gcacgtactg gtaaccaaag taaccacctg ctgagagcaa ggcaaggaat acaaggaatg
    17041 aaatcacaaa agctttcaca tgtgacttct tctctttctt aggcttaacg ggttttgctc
    17101 cttctctacg gctacgacgt gatgtatctc tgatgatatc cactgtttct gtagcaggcg
    17161 taggtgtttc tggacctgct tgttctgctt ttttctctgc cgtcgtatag gaaacagcta
    17221 cccttgttgg ggtttcattg tattctcttt caagtttctt aggtctaaca ggacctggac
    17281 ctggtcttga tccactttct tccgctggag aagaaggtac atcttgactt ggatgacttg
    17341 gaacaccagg agtttctctt tgaatctcat ctgctggaga agctggtaca ccttgacttg
    17401 ggtgagtagg cacggtagga gcttttctca taatctcctc taccgttgac aaggaatcag
    17461 ccatgagttc ttcagttgaa ggttcatttg caggagtgcg aactactgcc tcatcttctt
    17521 tcagaacttc atcatagcct tttacttttt ctaaatctct cagaatctgc tctttaaagc
    17581 ttaatttctc ttcttctctt gacttttcac tcaaaagttt ttcctccttg ttgataatcc
    17641 ataatattat atcttaaaag tccaaaaaaa gcaatctatg atacttttcc taacttattt
    17701 tttcatttcc cagaccatat cataccatgt ttcccctgca aaggttgact gggactggcc
    17761 ttcgttgaca aatccatgtt tttcatagta ggcgatgaga tagtcatgac aggttagatt
    17821 aatgccatct ctctcatctt caagagcaac ttctttcaag gctgtcagca atttctgacc
    17881 cagtccgagt ccctgtgcct ccttggcaat agacagacag gtcacagaga tataaccacc
    17941 aggctcatga ctatagtctt ttatttcttc tgtaaaagac tggtcttgca gatggcggtg
    18001 caggccaact ggtccttcga tataacccat gattcttcct tctttttcag caaccagaaa
    18061 agaggtctga atttctctca aatgtgcttc aaagacagaa ggaggaatgg cttcttcgac
    18121 cgaaaaattt tcaaattcaa gttcaacaat ccgatccaaa tcttctaatc ttgcttgtct
    18181 gattttcatt gttcctccag ataaaaggga ttaaaccaaa tcatactata gccctggcta
    18241 gttgcataga gcaaagttgc ttcttcatca acaaaaccgt tcatttcaaa ataggaaagc
    18301 agctcatcag gactctccaa acgaatccct ttgtaatcca gctcaactgc cacctctttc
    18361 aaggctgcaa gaagaagtgt tcccaggccc tgtctctgat ggtcagactc gatgactaaa
    18421 gaatgtactt ttagacattg cggattgtct gactggggac ttgataaaat atagcctaaa
    18481 agttgatttt catccctagc tagaagaaag gtatccgcac acttacggat actttcttct
    18541 aaaatatggg aaagttgctg cttttcagct ggaaaagacg aggtctgaag tgcccctatc
    18601 tcaggcaaat cagacttgct tgcctgaatg atcttaattg gaatttccat gggaacatcc
    18661 tattgaacat tgcttgtcaa gttagacaag agacgctcaa atgagtattc ataggtttgg
    18721 atgtctcctg ctcccataaa gacgtaaaca gcattgtcat ggtctaggag tggagaaaca
    18781 ttttcaacag taatcacttg gtgttttttg ttgattttgt tggctaggtc ttctacctta
    18841 acgtcaccat gatctacttc acgagccgag ccataaattt gcgctagata aacagcatct
    18901 gcttggttta aagcatgggc aaagtcgtcc aacaaggcaa tggttcttgt aaaggtatgc
    18961 ggttgaaaga ctgctacaat ttccttgctt gggtatttct gacgagccgc atccaaggtc
    19021 gcaataattt ctgttggatg gtgggcaaag tcatcgataa tcactgtatc attgacaatt
    19081 ttctcagtga aacgacgttt aacaccggca aatgttttca agtgctcacg caccaagttc
    19141 aaatcaaatc ctgctgtgta aagaagacca ataacggctg tcgcattcat gatattgtga
    19201 cgaccaaagg ttggaatgtg gaattgcccc aagttttgtc cacggaaatg aacggtgaag
    19261 gttgaaccag ttattgaacg aagaagatca ctagctacaa agtcattgcc ttcagcttca
    19321 aaaccataat aataaattgg tgcatcagac gtaatcttac gcaattcagc atcttcacca
    19381 tagacaaaaa gacccttggt gatttgtttg gcatagtcgt taaaggcatt aaaaacatcc
    19441 tcgagacttg tgaaataatc tggatggtca aagtcaatgt tggtgataat agagtattct
    19501 gggtggtaag gcatgaagtg acgctcatat tcgtcagatt caaagacaaa atatttggca
    19561 ttggccgaac cacgacctgt cccatctcca atcaagaagc tggtatctgt aatgtgagac
    19621 aagacatgag acaacatacc tgtcgttgaa gtttttccat gtgctcctgc tactcccatg
    19681 ctaacaaagt cacgcataaa gctacctaga aactcatggt aacgtttgta gctgatacca
    19741 ttttggtccg cataggcaat ttcgacgttg ttatctggac gaaaggcatt tccagcgata
    19801 atttccatat caccgtctag atttttttca tcaaaaggaa gaatggtaat tcctgcctgc
    19861 tcaagaccgc gttgggtaaa gtagtacttt tcaacatctg atccctgaac cttgtgcccc
    19921 atctggtgca acatcaaggc caaggcactc atccctgatc ccttaattcc gataaaatga
    19981 tatgtctttg acatgttttc tcccctattc tgtcattctg gtcagattca actcttgggc
    20041 aacccgacgt tcttgttctg tttgtttact ttttttattg tagatttggc tcttctttag
    20101 aaaatcataa ttgtttttct ttggagcagg tgctgacact tcttcattct tggtagggat
    20161 agaatgaact tcttccgcca agatataatg agactgggtc aatttttggc tatatttgac
    20221 aaattcacca ggattttcct tttggaaagg agctgtcggt tgattgccct gtctaactag
    20281 actgggctga gaatgacgtc tcgcaaggct gaaatcctga gttaggtagt tagcagagcg
    20341 tttctttttc aagtccgcac gcgcttcttc acgcgccacc tccgcatagc tctttccttc
    20401 ttttttaacc cctaaaggag cctttttagg tttttcgact tgcttttcaa tcggttttac
    20461 tggtttttct tcagcaatag gagcccattc taaataattt ttatctcgat actcaccctt
    20521 gatattactg atcagatcag actcatcata gagattcatg actggcattt cagtcaacat
    20581 gacctcgtca tctgacacca atggaaatcg ttcttgtttc attttctatt tcctttcaac
    20641 acttcattat agcgtattgt cttgattttt caagtgctgg cttcagaaat tcccaaaatt
    20701 tctctaattt ctgctagggt cagactacca cgtgactctg tgccgtccaa tacttgtgac
    20761 accagatgtt tcttttgttc ttggagttcc tgaatttttt cttcaatggt tcccttggtc
    20821 accaagcgat agacctcaac cgtttcttcc tgacccatcc gatgggcacg gccaatggct
    20881 tgcgcttcca ccgcaggatt ccaccaaagg tcaaccaaga tcactgtatc tgcacctgtc
    20941 aggttcagac cgaccccacc agccttgagg gaaatcagaa aggcatctct ttctccttgg
    21001 ttaaaggcct tggtcatgtc ttgtctttcc ttggctgggg ttgaacccgt aattttaaag
    21061 gaagtcaggc ccaagtctgg cagttcttgt tcaatttttt ccaacattcc cttgaactga
    21121 gagaaaatca agacacggtg tccgccgtct gccacctgta ccaataggtc tcggagacta
    21181 tctagtttgc cgctggctcc ctgataatct tccataaaca gggcaggagt gtcacatatt
    21241 tgacgcaagc gcatcaaacc agataaaatt tccacacgac ttcgctgaaa ttcctgttct
    21301 gacacttgag ccagatggtc tcgcatctgt tgtaactggg caaggtaaat agccttttgc
    21361 tggtcttcca gttcattttt ataaaccacc tcaatcaagt ctggcaattc agtcagaact
    21421 tcttctttct tgcgtcgcat cacgaaaggc ttgataaact gagccactcg ctctgctggc
    21481 aatttcataa attctttctt gcttggcaaa agtccgggca tgacgatttg gaaaatagac
    21541 cacaactcac ccagatggtt ttcaatcgga gttcctgaca aggcaaagac cgacggcacc
    21601 acaaattgtc tcaaggtctg ggcaatcttg gtctgggcat ttttcatgac ctgagcctca
    21661 tctaagaaaa ggaagtcaaa ggccatccct tgataaaact cactgtcctg acggaaggtg
    21721 gcatagctag tcacatagat ttgatggctc tcggcaagaa tctcctcacg acttgctttc
    21781 aaaccatgaa caacagtcac atccaactgt ggagcaaatt tctgaaactc atctgcccag
    21841 ttgtaaatca aacccgacgg agcgagaatc aaaacccgac tttcttttgt cacttgacta
    21901 gtcaaaaaag caatggtctg aagggttttc ccaagtccca tatcatcagc caaaatccca
    21961 ccaaaaccat aatgatggag catctgcaac cagccaattc ccttttcctg ataatctcgc
    22021 aagtcagcct tgacctgagt tgcttgcaaa ggaaagtcct ctggatgcgt caaatcctgg
    22081 gccagattct ggaattcttg tgaaaaagaa acacggtctc gcccttcaaa gagatgagct
    22141 aaactgtagg ccaaggattt ccgagcctgc aaggtcccat cttttaattc aaattgcccc
    22201 agttcctgta gattttggcg aattttcttg gtttcttcat cgaaaaagta aacttgatta
    22261 gacgaatcaa tataaaaatc ctgattggca accaaggcct gcatggcttg gtcgatttcc
    22321 tcctgggcaa tatcttgaaa atcaaactgg atttccaaga gacctccctt ggaggcaatt
    22381 tgcacctgag gactcgctag gctataaagc tcttctagtt tatctgatag gtcaacatgc
    22441 ccgagttttt caaagactgg aatgatatca tgaaaaaaat gatagacaga ctccgctttt
    22501 aaggcctgac gccaagattg aaaatcggct tcaaagcccg cagccaaaca gacttggaaa
    22561 attctttctt ctaagtccgc gtcacttgaa aatggtaatt cttctagctc ttgtcggcta
    22621 gatacctgtc tatttccata atcaaactga atttctaaac gaatccgatt atcttcttcc
    22681 ctgtcaaagt aaaaagaggg cgcaaaagtt ttgatttgta gacgttctgg agctgaaacg
    22741 gggcccatct ggataaaaag agtcagacag gaggccaatt tgtctcgatc actgctatca
    22801 aattgcaggt atttctttcc ttgttgaccc acaggtaacg ctttaatttc cttgagaaga
    22861 cgcatctgct ggtctgttaa aaaataaacc tgacctttat ggaaaagtac tgctccttga
    22921 taaaagacat tgaccctagg actctcactg atttccattt caaaatagtc cgaatattct
    22981 gttactgtaa aggcaaatag attggcatca gcatgcatat cctgaaaaag cagggtttgg
    23041 tagctatcca cttgatggtc aaattgaaaa tggggcaagg ccatcagtaa attcacaccc
    23101 tgctcaaaaa aggtcagagg gaaaaagagg tgccgacctt ggttttggaa aaagaggtct
    23161 ggaaccagcc cttcctccgt tagtccgtgc aagaaagtca aaagttcttg gctggcatca
    23221 tcaaaggctt cccaagaaag agactcctca taaatcttgc caatcatata cgactttctc
    23281 tgctcgacaa tccttaaaaa aagtggaata tcccgaatga catagtattt ttggctattg
    23341 atttggccga ttctcagagt ccacaagata tgattggttc ctgcttccac ctgacccaca
    23401 gctgataact cataggcgca ttctgatttt ggagataaaa ttcgatccaa aaacttgcca
    23461 cccaaggtca ccttggtttc aacggcctct ttttcttcat gaccttcttc cagactccac
    23521 aagatttcct gaccacgctc atcatttttc agaaaatgct ctagcgctgc caaatgcaca
    23581 cagtagcccc tcttttgaaa aaaatcacag gcacaaaaaa tcaaatcatc ctctaaacta
    23641 tagcgcagtt cttcttctgc aacgcgagcg tagagccgat tgttcttttc cttgatgata
    23701 tcaaccttac cagtttcata aagggcaaca ccttcgatac gaattttccc cggaatcaat
    23761 ttagccatat tttcaccttt accttatctt tttattatac catattttcg cctatgaaaa
    23821 tagccttcta ggaagacttt tctcctagaa ggctggattt ttaacatttg gcaaaagtag
    23881 ccacaatccg ctgacagact tcttgcaaca gagatttggg catagctata ttgatgcggg
    23941 catggagact tccttcctct ccaaaatcca aaccacggtt gaggataacc ttggcttcat
    24001 ttctcaacaa ctcttgcaat gtttcatcag tcaggtcata agctgaaaag tcaagccaaa
    24061 tcaagtaggt accttgcggt ttcatgacct tgattttagt ctcttttcca aatagatcca
    24121 ccacataatt gatgtggtct tcaaagactt gcttgagttc ctctagccaa tctttaccgt
    24181 atcgataggc agcttctgtc gccaaataac ccaagcctga aatttcatgc tgattattgg
    24241 ccaacaggcg tttctggaaa gccagtctca acttaggatt ttcaatgact gcataggaat
    24301 tttttgttcc agcaatatta aatgttttag tggcactgct caagacgata gcaaaatttt
    24361 tgaaggcagg attgatggta ttgaaagact ggtgtttgtg accaaagagg gtcaaatctt
    24421 ggtgaatctc atccgaaact aacaaaacac cgtgtttttg gcagagttgg ccaatcttct
    24481 ccaacacttc tttttcccaa acacgtccac caggattgtg agggttgcaa agaacataga
    24541 gtttaacctc ctcttccacc aaatcctttt caagttggtc aaagtcaatc tcaaacagac
    24601 tatccttttc cactaaggaa ttagtaatca atctacgatt attcaacttg acactgcgag
    24661 caaagggtgg gtagacaggc gtgttaatta aaaccgcctc gccttctttt gtaaaggttt
    24721 gaatagctgt tgagatggct ggtaccacac cctcgataaa gacaagagcc tctttgtcaa
    24781 agttgtaacc gtattgtgta gcttcccact tttgaacttc cttaattaag tcttcactgg
    24841 cataggtata accataaacc agttggtctg cgtaagtttg cacggcttgg tggatttcag
    24901 gcaagactac aaagtccata tccgctatcc aagctggtag aacttcacta tccgtttctg
    24961 tttctttcca tttataggta tggtgcccta aacggttggg caggcttgta aaatcatatt
    25021 ttcccatctt tgtcttatcc ttctaaggct tggcgcaaat ctgcaatcaa atctctagca
    25081 tcctcaatcc caatagacaa acgcaagagg tcatctgtca aaccataaga atggcgtact
    25141 tctgctggaa tatcagcatg agtttgagtc gttggataag taataagact ttccactcca
    25201 cccaaacttt ccgcaaaaga gaagaccttg agactgttca aaatatgagg aatgcgtgtt
    25261 tcatcggcta ctttaaagga aatcatgcct ccacgaccag tgtagagaac ttccttaact
    25321 gctggagaat ccttcaaaaa ggcaaccact tcttgggcgt tagctgttga gcgctccata
    25381 cgaagagaca aggtcttgag accacgaagc aactggtagc tgtcaaatgg agacaagact
    25441 gcccctgttg tattaagatt gtaaaaaagc ttctcgtata gttctaaact attggtcaca
    25501 accactccag ccaagacatc attgtggcct gctagatact tggttgctga atggagaacg
    25561 atatctgctc catcttcaat cggacgttgg tagatagggc tatagaaggt attgtccacc
    25621 accactttgg cacccttagc atgagccaat tttgctagtt tttcgatatc aaattccaac
    25681 atcaagggat tggttggggt ttcgatatag agaacatcca catccttttc taactcggca
    25741 atcaactctt cttctgtatt ggcataggta aaatggaaat gaccttcctg ctccacttgg
    25801 ttaaaccagc gaaaagaacc accgtaaaga tcacgcactg ccaagacctt acttcctact
    25861 ggaaagacgc taaaggccag tacaatagct gacatccctg agctagtcgc tagggcatag
    25921 tctgctgact caatagccgc caagacttcc tcagccttac tacgagttgg atttttagtg
    25981 cgcgtatagt caaacccagt agatcgacca aactctggat gctgataggt cgttgaaaaa
    26041 tgaagtggtg tcaccaaagc acctgttgcc tcatcagact tgatccctgc ttgtgctaaa
    26101 attgtgttaa tgtgtaattc cttgctcata caattcctcc aaatctatag taactattgt
    26161 accacttatt ttcatcaact cattttcttc ttttcaagag ctagttatag tttcaaacta
    26221 tataaaaagg gagtttttcc tgctcccttt aatagactat aaaatggtga atctcaaaag
    26281 acaccttcac tctatcattt gctcctgcac aaaacgagca taacgctcat gattttccag
    26341 tagttcctta tgagttcctg agccagtgat tttcccctcc tctaagaaga aaatacaatc
    26401 cacatctttt accgttgaca aacgatgcgc tataatcaca accgtcttct cctttagtac
    26461 agaatagagg ctactgataa tcgcatactc agaatccgca tcaagattag cagtggcttc
    26521 atcaaatata agaatttcag catcttttaa gtaggctcta gctatttgaa gtctttggct
    26581 tcgcccccct gacaagagtc gtccgcgttc accaacttca gtatctagtc cctctttcat
    26641 ggagcgaatc tcatcaccta gtgatactaa gtctagcact ttcatcaatt catcatcagt
    26701 tactaagcga ttcaaaccga gacaaagatt gtcacgaata ctgccagata agactgcatt
    26761 attttgtgaa acccaagcga ttttacttct ccattctttt aagttaaaat catatatact
    26821 tgattgctcc attagaatat ctcctgaaag cggtttataa aactgctcta acaaacgcac
    26881 aatcgttgat tttcctgatc cagatggtcc aacaaaagca attttttgcc ccttgaaaat
    26941 tgaacaagta atatccttta agacaggtcg attttcatca taaccaaaat agacatggtt
    27001 aaaattcaac ccttgtcctg ataccgattt tcctccctca aatttttctt taggaactgc
    27061 aagcaagttc tccagtgcaa ctgaagatcc cttgctccta gaataaacag ttacaaaatt
    27121 agctatatta ctaataggat taagtaattg aaagaggtaa atcaaaaacg aaaccaaggt
    27181 tcccacagat atatatcctg cgctgacccg ataaccccca taggttagca tcacagctat
    27241 agtcgcaaag ataaataaga gagcaaacgg ggtctcaaaa gaagtaaccc tatctgattt
    27301 cagtgaattg ttttgtaccc tttcaataca attatccaaa acatcctgta cacttttctc
    27361 tgcttggtta gtcttaatta attcatgttc ttgaatcttt tcagtcaatt gccctgttaa
    27421 aaatccgatt tcctcctgta aacgacgact atacttttca ctgatattgg aaaggggcaa
    27481 gataataaac atcatacaag gaagagtgat gaataaaagt agagaaagat tccaatcaag
    27541 actaaataag actacaatgg aaccaagtac cataactaaa ctcagaataa tatttgggaa
    27601 agtcgtaatt aaaaactcac gaatgacact cgtgtcattg acaatggcag aagtcaactc
    27661 cccactttgg ctcttatcaa agaaggattt ctctgcataa atcaacccct ctgtcacttt
    27721 tttcctgatt tttgctatct ttttttcacc cgattgacta aacagatagt aaccaataga
    27781 agaaaacaag gcttgaccaa taaaaatcaa aaacgattga aatactttgg aacctatatt
    27841 ttcaatagaa ctcccatcta ttaaaccctt taagataagg ggaagcaaca aagcaagtag
    27901 actagacaga acaagtaaga aactccccat aatcacctta gtatctactc ttaataattt
    27961 taatttcata aatactcctt ataatatttc aacggataaa gtcgggaata actcaatttg
    28021 aggataaaat ctaataaatc ttcctataac aaaacgcata acatctagga ttttatatac
    28081 ctgatattat gcgtttttaa gcacaaagac ttcttacaca aacttatcta caattagatt
    28141 ttatttgaca tgttttgcca attcttcttg ggctttttta ttggattctt ctttttcttt
    28201 caaccatttt tctctggctt ttgcatattc gtctgttgtg acaatcttat cttgtacttt
    28261 gaggtattta tatgattcaa ccccttttgt accggttaaa ccataggcag cagcaaatgg
    28321 tacggttctt ctcaatgatg gtgttccccc acgcgaaaca cttggaagaa ctaaagaact
    28381 atcaatcaac caagcttgaa tatcagcata tttctcataa cgtttggctg gatcttgctc
    28441 tttattagct tcttccaaca ttttagtata gacatccagt ccaactgcct tagccttgtc
    28501 attggcttct ccaggctcta gtccaagatt ttgtagaact cctccactat tagtattaaa
    28561 aatatcgaga taggttgacg ggtcttgata atcaggtccc caaccgccat gatataaatc
    28621 atagtctttc tgagcagctg tttgagcaaa gtagcctgaa ctgtcaaact catctgatgt
    28681 taattgctga atgtcaatca ctacattatc agaacctaaa acagattcaa ttgattgttt
    28741 gatagaacta actccttgta tgcctacttt atctgttact tccacagtct tatccaagtg
    28801 gattgggaat tgaacaccct ttgcttcgag ttctttctta gcttccgcaa acttagcctt
    28861 ggctttctca ggattgtagt aagggtcttg accatccgca aagttgatac cttgccattc
    28921 cttaccatag ttgaccatct tagaggctac aacttcacca aagtcttttc ccttgatact
    28981 gacaaagttt ggaggaacca ctaggttacg caaaatcttt gttgcacctt ctttcccttc
    29041 agactgagcc ccataagatg ttctgtcaaa agcaaaattg atagcctgac ggaaattttt
    29101 attgagaact gcttcctgag tcgatttctt ttcaatgtca cttgttttag aagtataatt
    29161 gtaagacttc ctatctaggt taaaattaaa gaaatatgaa gttgaatttt gcatactata
    29221 gatgatattg tttttgtatt tttctttaat cccttcatag ctggagctgt taggaaaaag
    29281 acgagccgta gtataagcac cagctgtaaa attacgttcc agtgattctt ggtcgctacc
    29341 atcatagtag gtcaatttca catcgtctac aaagacattt ttagcatccc agtagttagg
    29401 atttttcttg tattcgatga cagattttga aacaaaagct ttcatcaaga aaggtccact
    29461 gtacaaaata ttagcagggt ctaccttgcc aaaatcatcc ccttttgatt tcaagaaatc
    29521 tgcattgact gggaaaagaa tggttgaggt tgtttttgag ttccagtaag gttctggtcg
    29581 agccaaggtg tattgaacgg tttgatcatc aagagccttg ataccgacag ttgaaaagtc
    29641 gcttgtttta ccagtgatat agtcatccaa acctgcaaca gagtcctgca ctagatacaa
    29701 ggcttctgat tttttatcag ctgcatattg caatcctgtc acaaaatcct gggcagttac
    29761 aggtgcatat tcttctccct cagaagtaaa ccacttggca tccttacgaa gtttgtaggt
    29821 ataggtcaaa ccatcctgag aaacagtcca atcttctgcc aaggatggca catagttccc
    29881 atattggtca ttttccatga gaccatctac cagattggtc acaatatcat tggttgttgc
    29941 gcggttttct gctagatagt tcaaactaga tggatcactt gaataaacat agttataggt
    30001 ttttgatgca gtgctagaat ttccacacgc gctcaataaa actcctgtac ccaggacaag
    30061 acctgccaag gttagatatt tgctcttaga ctttttcatt tccagaacct cctgctttaa
    30121 atatttgttt tattatacaa ctctttgttt gtttagtcaa ttgttttttc caaaaaatcg
    30181 aaaaaaacct cattttctat ttttgaaaga taattttagc tattttgacg ctaaaaaaag
    30241 gaataagttt caagttctcc taaaagattg ttcaacttat tccttttatt tataaatgcc
    30301 accttacgaa agcttaaatt ttgctcgcag gcgatctgct tggacttttc ctattacctt
    30361 atctaataaa taggtacgca gactcataac catataaagt ccacccccca tggcaccgac
    30421 aagagctaca taaaagaagc tccacaaacg tccacttggt tggaagaaaa atcctaacag
    30481 ccactggatg gttcctatta acagaaacat gactagggtc agcaaactga ttaaaatggt
    30541 tcgcttcaaa atcaccttgc gcttgacacc agttacttta caaatatccc gatacatcaa
    30601 gacgttagga atgatgagag caatggttgt tgaaatcaaa ggaccataac tgtggaagag
    30661 ggcgatggta ggtagttgca agactagctt ggcaatagaa ccatagataa aatagagaac
    30721 ggccttgcgg ttgcggaaca tggcctgaag cattggagac aagaccatgt acaaacctaa
    30781 aataatagac tgcaaaactg caaagacaaa taagcccata gccaaactat ctggcttacc
    30841 atagaagacc gtataaagag gttctcctac cataaccact ccaaccgttg ctggtagcaa
    30901 gaacataaag agtagggtga gactgtcctg aaccagacga gaagctgctt tcaagtcccc
    30961 cttgacatag ttttccgtca aaagtggcaa accaacactc ccaatcgaaa cccctacaga
    31021 aatcaaaatc atcgtgattt tattaggatt ggctgagaaa taagaaaaca tgacaaccaa
    31081 gtcctcattg ctgtagttgg taaaccagct catactattg ataaaggtca gctgatccaa
    31141 aatctggaag agctggatgg cagaccctgt caggataaaa ggaatggctt ccttaatggt
    31201 atcaaccaag agacgcttac tgttaatctt atctcctgtt tcaaagactc ttttgagtga
    31261 accttcttgg gcaaggaaat aaatcaagac tgcaaaactg gctaccatac cgacaaaggc
    31321 agcaaaggtt gattgggtaa cggctgctag ataatctcct gaaccgagct tcataatgat
    31381 aaaggttgct aggagcatcc agataacacg aatgacctgc tcagcaattt ggctcatggc
    31441 atagggtttg aggttattca tcccttggaa aaatcctcgg ataacactca tagacgggaa
    31501 aatcaagact ccccaagcca agctttgcat gattgggatc aagtctttgc ccacgccaga
    31561 caagtctgct agccaaggag caaagacata caagactaaa gcaaaaacca ggcctagtcc
    31621 tgtcataaag cctaagaagc tccgaatcag ggcaaagcta tgctcttctt ctcgcatggt
    31681 attatacttg gcaacttgct tggccaccgc aactggaatc cccgctgttg aaaccaacaa
    31741 gaaccaagca tagatattgt aacccatggt aaagagacca tttgccttag ctgcataagc
    31801 ccccatccag atgtaccaag ggataatgta aacagccccg agtaggcgac tgataaagtt
    31861 actagccgtt agccaagcag tcccccgtaa catctgggcc tgctggtgat tgttttcgtg
    31921 cgacatagat tcctcattca attttgataa ctaggaaaaa ctccttatct tccattataa
    31981 acttttcctt gttacttgta aatttacgac tttcggttta caatagaaag tatgattaag
    32041 attgaaaccg tattagatat tttaaagaaa gatggccttt ttcgcgaaat tattgaccaa
    32101 ggtcattacc actacaacta cagcaaagtt atttttgata gcatcagcta cgacagccga
    32161 aaagtaacag aagacactct tttttttgca aaaggcgctg cctttaaaaa agaatacctt
    32221 ctttctgcta taacacaagg tttagcttgg tatgtagctg aaaaggacta cgaagtcggt
    32281 atccctgtca tcattgtgaa cgatataaag aaagccatga gtttgattgc catggagttc
    32341 tatggtaatc cacaagagaa actcaaactc cttgccttta ctggtactaa gggtaagaca
    32401 acagcaacct atttcgccta taacatctta tctcaagggc atagacctgc tatgttgtcg
    32461 accatgaaca caactcttga tggcgagact ttctttaagt cagcgttgac aacccctgag
    32521 agtattgacc tctttgacat gatgaatcag gctgtgcaaa atgaccgtac ccacctcatc
    32581 atggaagtct ccagtcaagc ctatctagtc catcgagtct atggactgac ctttgatgta
    32641 ggagtctttc ttaacatcac tcctgaccat atcggcccga ttgaacaccc tagctttgaa
    32701 gactatttct accacaagcg tctcttgatg gaaaatagcc gagcagtcat cattaacagt
    32761 gacatggacc acttctcagt cttgaaagaa caggttgaag atcaagacca tgatttctat
    32821 ggtagccaat ttgataacca aatcgagaat tccaaagcct ttagcttttc agctacgggt
    32881 aaactcgctg gagattatga tatccaactc attggcaact tcaaccaaga aaatgcagtt
    32941 gctgctggac ttgcttgtct ccgtctcgga gcaagtcttg aggacatcaa aaaaggcatc
    33001 gctgcaaccc gcgttcctgg tcgtatggaa gtcctcactc agaaaaatgg agccaaggtc
    33061 ttcatcgact atgcccacaa tggggatagt ctgaaaaaac tcatcaatgt ggttgaaact
    33121 catcaaaccg gaaagattgc tctggttctg ggaacaacag gaaacaaggg agaaagtcgt
    33181 cgtaaggact tcggcctcct cctcaatcaa caccctgaga ttcaagtctt tctgactgct
    33241 gatgacccta actatgaaga cccaatggcc attgcagatg aaattagtag ctacatcaat
    33301 catcctgttg aaaagattgc ggatcgccaa gaagccatca aggcggcaat ggctatcaca
    33361 aatcacgaat tagatgcagt tattattgcg ggtaagggag ccgattgtta ccaaatcatc
    33421 cagggcaaga aagaatccta cccaggagat acagccgtcg cagaaaatta tttataagaa
    33481 tagaaaaaat caagggaaaa tgaaaccctt gattttttcc tattatttag taaaaacgtt
    33541 tttcaaacct tcaacggcac cttctacagc accttttgcg tcttctacaa cgtcttttgc
    33601 cttagcaact gttttttcaa cagctccttc aagttctgtc ttgctgtctc cagtaacttt
    33661 accaaaacct tctttgacag cgcctgttgc ttgttccaat ttgttttcaa gtgacatatg
    33721 attgtctcct ttagtttatt ccgatatgtg ttaccggtta catatagttt ataccgaata
    33781 ctaaggaaag tcaaacaatg tgctcagaaa caagaaatca cgctaagtag aaagttaatc
    33841 tctccttatc aaaatcgata gccaactcat actttctatc ttcattttgc acaatatagc
    33901 ctaagacaac caaactgtcc acaaagatat cacggcattt ctgcataagc tggtcttttt
    33961 tgagaaattt caacaaaaaa gccgtcatat acttgagtgc atactcagga ttaacatctc
    34021 ctaaaatgtc atagagctcc tgctgttttt ctgtcaaagg atactgctgt ttgaccttgt
    34081 aaaaataatt ggacagggtc attttaatgc gcgcaaagtc cgtctttcaa ctaaaatagc
    34141 tgcattgatt tgattgcgca attccgtctc aaaactctac tccaacaagg cttgatagac
    34201 cggactagct tctctgacaa aaatctcttg atccagttca agactatcaa gtgattcaag
    34261 cgtaggaaaa ttgaggtaat agcgcttatt ttcacgtaga atcaagcttg cctttatata
    34321 ctcttccatg agtttatcaa ctgcaacatc tggaaattga gccttaattt cccgtagaat
    34381 cacatcctca tactggtcca gatagcggat caattctcca aaaatggctg tctcgtcaaa
    34441 cgagatggat taaaaatctg aatcatgaag attcctttct ttacattgta acagaggcga
    34501 tgctgccaac tgactaggat tggtcccagc ttggttcaga ggaacaggca gacctagttc
    34561 tatgtaatac tccctccaaa aatcactaat ctcctgttcc ccatctccaa atttcatagg
    34621 aatagtttca aaaataggta ggatttctgc tatatactgg gagcagtaga aaccagctgc
    34681 atctggatag aaagaagcat tgtagggtgc tccaagatgt ctgcaagctc tttccttcac
    34741 cgactggata tccatttctg ggtaaacata gaggtcgtac aaatgattgg actcaaagaa
    34801 gtctgccggt tcttgacaga caacaccagc ctgtccacta gcatgataaa tcatcccatc
    34861 caaataaatg gcaacatggc tatagttacc tgtggaagtc tggatggcct gtcccatgtc
    34921 tgacccatct ctcacaaaaa tcaaatcgcc attttctaac atacttcact cctagaaaaa
    34981 aaggagccga aactcctttc gatataaggc aaactagact ttccgatttt gagctgacac
    35041 ccaaaatctt acgcattaaa gctttcagtt aattgaggta ccacttgttt cttacgtgaa
    35101 acggcaccag caaggaaggc atgattgttt tcaagtttga aattgaaagc cgcttcgacc
    35161 ttgtccatat tggcaccaag agccaatatt tctgagtttg agttgacgat atctgtaatc
    35221 atcaagacaa agtcagagta gccgtttgat tcgttggcag cttgcattgc agcttcaatt
    35281 tctgcttggc gttccaaaac ttcagcgatg tcaactgtgt tcacttgggc aacacggaca
    35341 ttatttccgt tgagttcaaa agtcttagca tcgatgtcaa tcaattcttc agcagatttg
    35401 ctagccaagt tggtaccagc tttcaacatt gccaaaccat attcttccaa gttcacacca
    35461 gccaattcag ccaattcagg agcaatgatt ttatctgttg ggtgtgttgt tggtgatttc
    35521 aaaagaaggg tatctgaaat caaacctgaa agcatcaaac ctgcaatctc tttaggcaca
    35581 gctacaccat gttctttgaa catacggtaa acgattgaag acgctgatcc aactggctcc
    35641 aaacgcatgt aaagtgggct tgcagtttca aagttagcca cacggtggtg gtctacaaca
    35701 ccgtaaactt ctacttcagc gatatctgat acagattgtt ggaattcatt gtggtcagtc
    35761 aagataactt gctctgcccc ctctgctttg gcagaagtga taacacgtgg tgcttccaca
    35821 ccaaaatagt tcaagacaaa ggctgtttct tcatttggag ttccaagggc aacagcttcc
    35881 gtatccaaac cgtaagcttc ttttgcaagg taggcaaaag ctacagatga tccgatggca
    35941 tctgagtctg gattttggtg accaaatact agaatcttgg acatgataat acctcatttt
    36001 gtttattttc tttattgtag catttttttc gctttttgac aaaggtaaga ggagtcaaag
    36061 gactccccac gattcttcaa aataactgag taaatttcta tcttgatttt cagataaaaa
    36121 ttcttccttc tgtggcctct tcttacgctt gagaagagct tcagccgaca tggcttcttc
    36181 cttactggca aaaccttgag catagataag tttgactggc aagcgtgctc gtgtatattt
    36241 ggctcccttc ccactattgt ggatagcgag gcgtcttctc acatcagtcg tatagcctat
    36301 atagtaggat ccatcacgac actccagaac gtacatataa gccttatgat ccataataaa
    36361 tctcttcgat ttcgggcgta taagagccat catcattgtg gacaatcaaa ggaggtaaga
    36421 ccttaaagcc acttgttgag ccatccttga tcgcctcaat caaaagcata ttggcttcct
    36481 tttctctttt tggataaaca aactgcaggc gcttaggggc tagattatgt cgttttaacg
    36541 tatccaaaat atccagaagt cgatcaggac gatgaaccat ggccaaacgc ccattagact
    36601 tgagaatact ctgggcacta tgacagattt cttccaaatt agtcgtgatt tcgtgtcgag
    36661 ccaagagata atgttcactc tcgttcagat tagaatgagg atccaccttg aaatagggtg
    36721 gattacacaa aatcatatcc accttactcc cctgaatgtg agcaggcata tttttcaaat
    36781 catcgcagat gacctgcatt tgttcctcta atccattcaa acggacagag cgttcagcca
    36841 tatccgccaa acgctcctga atctcaacag acaatatctg tgcttgagta cgagtgctag
    36901 caaaaagccc cactgctcca ttcccagcac aaaaatccac aatcaacccc ttcttaggaa
    36961 aacgtggaaa tcgtgataag agaacactat ccaccgaata actaaaaacc tctctatttt
    37021 gaatgatttt gatatctgtc gaaaagagct ggttaatgcg ctctcctgat tttaataatt
    37081 gttcttcttc catggtccta ttatagcaaa tttatattaa cattacaaaa aatataaaac
    37141 tctaaactac ttcttctttt ttaaatggtg cagggcttct ccagtccaga ttggtagcat
    37201 tcgtcgaaag ggagcaaagc cgtagttaaa gcggtcgctt gaaaagcgtc tccgtctagg
    37261 aaactggtac ttttcttcct ccaaagtgcg gatagaaaga ctggctttcc ctgtaaattc
    37321 atctaaatcc actacctgaa cttgaacctc ttcatcgact ttcaaggttt catgaatatt
    37381 ttcaataaat cctgtccgaa tctctgaaat gtgaatcagc cccgtatcac ccgtctctaa
    37441 ctcaacaaag gcaccgtagg gctgaatccc tgtaatacgc ccctttagct tatcaccgat
    37501 tttcatctta gtcctcgatt tcaatagttt caattacaac atcttcaact ggcttgtcca
    37561 tagctcctgt ctcaacagca gcaatggcat ccaagacagc gtaagatgct tcatcagcta
    37621 actgaccaaa aaccgtgtga cggcggtcta ggtgaggtgt cccaccttga ttggcataga
    37681 tttctgcaat cggttctggc caaccaccac gagtaatttc tttcttagaa taaggtaggt
    37741 gttggttttg cacgataaag aactggctgc cgttggtatt tggaccagca tttgccatgg
    37801 aaagagcacc acggatattg taaagctctt ctgagaattc atcctcaaaa gattcgccgt
    37861 agattgactc gccacccata ccagttccag ttgggtctcc accttggatc ataaagtcct
    37921 tgataatacg gtggaaaatg acaccatcat agtagccatc ttttgaaaga tatacaaagt
    37981 tagccactgt tttaggagca tgttcaggga aaagcttgat acgtaagtct ccgtgattgg
    38041 tcttaatagt cgcaagagga ccttctactg tttcaatgtc tacttgtgga aaatgcaatt
    38101 ctttttctac cataccaaat acttctaagg cagcaaaaat gccatcttct tctaatgttt
    38161 ttgtaatata atctgctttt tctttgattt tatcatgaga aattcccatg gcaacgctga
    38221 ttccagcata atcaaagagt tccaagtcgt tgagaccatc tccaaaaacc atgaccttct
    38281 ctggtttcaa gccaaggtgt tccacaacct tttccacccc cgtcgctttg gagcctgaaa
    38341 tcggcacaat atcagacgaa tgttgatgcc aacgaaccat gcgaagtttg tctgagagac
    38401 tgtcaggcaa gtgcaagtca tctcccttat cttcaaaagt ccacatctga tagatatctt
    38461 ctttttcatg gaaatcggga tctacatcta agtcgggata aattggattg atagcttcac
    38521 tcatcatatc ggtgcgagtc gacaacttgg catcatgact cccaaccaag ccatactcaa
    38581 ttccttcttg cttagcccaa gagatatact cctcaacatc tgacttttca atctgatgct
    38641 gataaatgac ctgacctttt ttatcttcga tataagcccc attcaaagtt acaaaaaagt
    38701 caggcttgag atcacgaatc tctggaacaa caccaaaaat gccacgtcca gaggcgattc
    38761 ctgttaaaat tcctttttca cgcaactgtt taaaaacagt gggaattgta gttggaataa
    38821 accctgtctt tgaattccgc aatgtatcat caatatcaaa aaagacaatc ttgatcttct
    38881 ttgccttgta tcttaatttc gcgtccatct cactacctct ttcaatctaa ctctttccat
    38941 tatatcataa agtaggcaaa tcccctattt tcaaaaagtt tatcattttt attttaattt
    39001 cttggatgag aaaagagaca tatttatgaa aaagctccat cgtgctttta atgtgttctc
    39061 ttgttttcaa actcgtaaaa agggagccac tgagcctaac tcgctctctc atttcaaagc
    39121 ttgtgaaaaa agacccgttg gggtcttaat tcgctttctt gttttcaagc tcatgaaaaa
    39181 gagacccaac tgggtctttt ctttaatctt cgtttacgaa aggcatcaaa gccattacgc
    39241 gagcgcgttt gatagctgtt gttactttac gttggttttt agctgaagtt cctgttacac
    39301 gacgaggaag gattttccca cgttctgaaa cgaaacggct aagaagctca gtatctttgt
    39361 aatcaacata ttcaattttg tttgctgcga tgtaatcaac ttttttacgg cgtttgaatc
    39421 cgccacgacg ttgttgagcc atgttttttc tcctttataa gtttagttgt ccattagaat
    39481 ggtaaatcat catctgaaat atccaatggg tttgttgctc caaatggatt ttcattacgt
    39541 gaaaagtctg gtactgaatt tgtaggtgct gaatagtttg cagttggtgc agagtaagct
    39601 ccacctgtgt gaccctcacg cacactacgg ctttccaaca tttggaaatt ctcagccacg
    39661 acctctgtca cgtagacacg ttgtccttgc tggttatcgt aactacgagt ctggatacga
    39721 cctgtcaccc cgataagtga gcctttttta gcccagttag caagattttc agcctgttgg
    39781 cgccacataa cgacattgat aaaatcagcc tcacgttcac cattttgact cttaaatgta
    39841 cggtttactg caagagtaaa agtcgcaact gctacatttg atggggtata acgcaactca
    39901 gcgtcacgtg tcatacgccc tacaagtaca acattgttaa tcatagttta ccttcttacg
    39961 cgtcaatttt gacgatcatg tgacgaagaa tgtcagcgtt gatttttgaa agacggtcaa
    40021 actctttaag agctgcatcg tcatttgctt caacgttaac gatgtggtaa agtccttcac
    40081 ggaaatcttg gatttcgtat gcaagacgac gtttttccca agtttttgat tcaacaacag
    40141 ttgcaccgtt gtcagtcaaa atagagtcaa aacgtgctac caaagcgttt ttagcttctt
    40201 cttcaatgtt tggacgaatg atataaagaa tttcgtattt agccattgat atgttcctcc
    40261 ttttggtcta atgaccccaa gactttgcaa ggggtaagtg aggttcgctc acaataaact
    40321 attatactag aaaaaatttt tttacgcaag taaaaacact agaattcgaa aaaacgccac
    40381 atgggcgttt tcctgttctt atggtttgat acggtgcaac atacgtggga atggaatagc
    40441 ttcacggata tgttttgttc ctgctgcgaa ggttaccata cgttcgatac cgataccaaa
    40501 tcctccgtgt ggaactgtac cgtatttacg aaggtcaagg tagaattcat attctgtacg
    40561 atccatgcca agttcatcca tcttagcgac aagggcatcg taatcttcct cacgcataga
    40621 cccaccgata atttctccat agccttctgg agcaagcaag tctgcacaaa gcacgcgctc
    40681 tggatttcca ggaactggtt tcatgtagaa ggccttgatg gctgctggat agttcatgac
    40741 aaatgttggc acaccaaagt ggtttgaaat ccaagtttcg tgtggtgacc caaagtcatc
    40801 accatgctca agatgctcgt agtcagcatc ttcatcattt tcatgctctt gcaagaggtc
    40861 aatggcttga tcgtaagtga tacgtttgaa tggctctgca atgtagcgtt tcaagagttc
    40921 tgtatcacgt tccaaggttt ccaaggcttg aggcgcgcgg tcaagaacgc cttgtagaag
    40981 agctttcaca taagcttctt gcaagtcaag cgactcatca tgtgtcaagt atgagtactc
    41041 agcatccatc atccagaact cagtcaagtg acggcgtgtt tttgattttt cagcacggaa
    41101 aactggacca aagtcaaaga cacgaccaag agccatagcc cctgcttcta ggtaaagctg
    41161 acctgattgg ctcaagtagg ctggcgttcc gaagtagtca gtttcaaaga gttctgtaga
    41221 atcttctgcc gcatttcctg aaagaattgg gctgtcaaac ttcataaaac cgttcttgtc
    41281 aaagaactca taagttgcat agataatagc gttacggatt tgcaacacag ctacttgctt
    41341 acgagagcgt agccacaagt gacggttatc catcaaaaag tctgttccgt gttcttttgg
    41401 tgtgattggg tagtcttgag attcaccgat cacttcgatg tctgtgatgt ccaactcata
    41461 gccaaattta gaacgttcgt cctctttgac aatacctgtc acataaacag acgtttcttg
    41521 gctcaagcgt ttgataacat caaacttctc aagtcccact tcttcaccaa atttttcgac
    41581 aaagtttggt ttaaaagcca caccttgaaa gaaggctgtt ccatcacgca attgtaagaa
    41641 agcgattttt ccttttcctg atttgttggc aacccaagcg ccaatcgtca cttcctgacc
    41701 aacatagtct tttacgtcaa taatcgttac acgttttgtc attatttttc cttttctttt
    41761 ttattcttta tggcaaacca cctctatatt gttcccatcc aggtcaatca taaaagcagc
    41821 atagtaaatc ggatgctcac ttcgataacc aggagcccca ttgtctcgcc cacctgcctc
    41881 taagccagcc tcataacaag cctgaacttc ttccttattt tctgctaaaa aagcaaaatg
    41941 aacaggatct tgtgttccct gagtcagcca aaaatcacca ccaggatgag ggctgttcgg
    42001 ggatagaaaa ctaattagag aactagtctt aaaagccaat ttatagtcca aaggagcgag
    42061 aaaactccta taaaatcctt ctgaaatttg taaatccttt accttaatct caaaatgatc
    42121 aatcattctc actacccata aatgctttca agcgttcgac tgcttcttta agcgtgtcta
    42181 ggtctgtcgc atagctgagg cggacatttt ctggtgctcc aaatccagct cctgttacca
    42241 aggccacttc ggcttcttct aagataacag ttgtaaagtc tgtcacatcc gtgtagcctt
    42301 tcatctccat ggcctttttg acatttggga agagatagaa ggccccttgc ggtttgacca
    42361 cttcaaatcc tggtacctct gcaaggaggg gatagatggt attaagacgt tcctcaaagg
    42421 cctgacgcat gctttctaca gtatcttgct cacctgatag agcctcaact gctgcatatt
    42481 gggctactgc tgacggattc gaagttgttt gacctgcaat cttggacatg gcagcgataa
    42541 tgtctgcttc tccaacggca taaccaatcc gccaaccagt catggcataa gttttagaca
    42601 caccattgat gaccactgtt tgcttgcgaa tcgcttccga taggctagaa atcggtgtga
    42661 actcatgacc attataaacc aagcggccat agatatcgtc tgctaggatg agaatatcat
    42721 tttctacagc ccagtttcca attgccaaga gttcctcacg ggtgtaaatc atacctgtgg
    42781 gattagatgg cgaattcagc accaaaacct tggtcttgtc agtgcgagct gcttctaact
    42841 gctctacggt caccttaaag tgattgtctt ccttagcaga aacaaagacg ggaacgcctt
    42901 ctgccatctt gacctgatct ccatagctaa cccagtatgg ggttgggatg atgacttcat
    42961 cacctggatt gaccacagcc ataaagaagg tatagagaga atatttggct cccgcagcga
    43021 ctgtcacttg atttgacgct acagaatagc cgtaaaagcg ctcaaagtag ctattgaccg
    43081 ccgccttaag ctctggcaga cctgaggtta ctgtataaaa agaagcacgc ccatctcgaa
    43141 tcgatgcaat ggcggcatct tggatatttt tgggagtagt gaaatctggc tcacccaagg
    43201 ttagagacaa aatatctcta ccctcagcct tcagtgcttt ggcacgggct ccagcagcca
    43261 aagtcacact ttcttccatt tctaaaacac ggttggatag tttcataggc cctccttgtt
    43321 gaccaatgct cctgtttcaa aatctactag ataaaaatca gatcctgact taacttccca
    43381 gattggctta tcttgataac ggccaaaggt tatcttgtca atctcgccag ctcccttttc
    43441 cttagaaacc gtttctgctt tttcttgtga aacaccctga tttagctgat aaacgtaaat
    43501 cttatggtca tctttaccaa tcaggacagc aagcgcttct tgctgtttgt tacgaccaag
    43561 aacgctgtaa taagattcca agccattgta taaatcaacc tgatcagcct gctctaatcc
    43621 tgcatactgc tgagctaatt tttctccttc acttttagct gtttgatagg gtttcatgct
    43681 aagagaaacc atatacagaa aggaaccact gataaccaca aacaaaatcg tcatccctag
    43741 accatactgc cacagtagat tattttttgc tttgttttgt ctttttttca ctcgtctatt
    43801 ttaccatcta ttaagcttta ttacaagtga atataagaat actcttcgaa aatctcttca
    43861 aaccacgtca gctttatctg caacctcaaa gctgtgcttt gagcaaccaa ttctatttct
    43921 cccttcaaac aaaaccgatt ttgaaagtga aacagttctt actttttcag tcacaaatga
    43981 ttagagtttc cgaaatttta caattttcaa ataaactctg agcaaatttc ttgcaagttc
    44041 ttttgttttg ttgtaatata ttttataaca acgagagagt tctcgaaatt ttaagaaaaa
    44101 ggagacacat catgtctaaa aaagtattat ttatcgtcgg atcactacgt caaggttctt
    44161 tcaaccacca aatggcgctc gaagctgaga aagcacttgc tggtaaagcg gaagttagct
    44221 accttgatta ttcagccctt cctctcttca gccaagattt ggaagttcca acacatccag
    44281 ctgtagctgc tgctcgtgaa gcagttctcg ttgcggatgc tatctggatt ttctctccag
    44341 tctacaactt ctctatccct ggtacagtga aaaacttgct tgactggcta tctcgtgccc
    44401 ttgacttgtc tgatacacgt ggcgtttctg cccttcaaga caagtttgtc acagtatcat
    44461 ctgtagccaa tgcagggcac gatcaacttt tcgctatcta caaagacctc ttgccattta
    44521 tccgtacaca aggcgttggt gatttcactg ctgcacgtgt taatgactct gcctgggcag
    44581 acggaaaatt ggttcttgaa gaaacagtcc taaactcact tgaaaaacaa gctcaagact
    44641 tggtcgaagc gattcagtaa aaaatagaaa attttagaaa gtctaaatca attatagcca
    44701 cctaataggg tggcttttta tttctcaatc aagattttca gacaaacatc agccatccaa
    44761 aaaaaccttg agcgttacca ctcaaggttt ttagcagtct tttccatcat aggaaaatca
    44821 ttctaagaat attctttcat tcgagatcta tcacttatca tcttctttcg agaaattagt
    44881 gactgctttt ggctgattaa gcttcttttc ttcgtcctac atataggctc aatagaatac
    44941 cgctacctaa aagaacagag atataatcag attggcttcc agtttcaggt aatttgtccc
    45001 cacggttttc tttacctgta gttggtgcta ctgggttagc atttactgga gttgctggtt
    45061 gagccggtgt tggtactact ggttgttctg gaaccgctgg cacaggttta accggtactt
    45121 ctggtgtcgg cacatttggt gttggaggaa ctggtttgtc atttgatggt gttactggtt
    45181 tgtcttggca tccacatttt ccatctttac cgtctttacc gtctttgccg tctcgaccat
    45241 cacgtccatc tctaccatct ttaataactg tttttgtcac agttccatca gaattgatga
    45301 ttgtgattgt atgactacca tcaccattat cttcaacaga aacttttggt gatttaccat
    45361 ctttaccgtc tttaatcacc atttctgtct tacttccatc tggattagtg attgtaacaa
    45421 cgtgagttcc atcgttatta tttttaacag ttacagttgg tgaaacgcta tctttgccgt
    45481 ctttaccatc tttgataata gatttgtatt cacgaccatc agaatctacg attgttaatg
    45541 tatgagttcc atctccgtta tcaacgatgt tcgcttttgg tgatttacca tcaaagattg
    45601 tagttgtcgt tcctttacca tcagcacctg tcactttaat agtgtgtgaa ccatcgttgt
    45661 tatcaataac ttcaagtttt ggttctctac catcacgaac tgtagttgtt gtagttgttc
    45721 cgtcaccatt tagaacagag atcttatgag ttccattttg ttcatcagtt atagtcactt
    45781 ttggtgattt accatcacga actgtggttt cagttgtcac accttctgga tttgtaataa
    45841 cgattgtatg acttccgtct ccattatcac gaacagatgc ggttggagta cgtccgtcaa
    45901 caccgtcacg tccattctta acaacaaact cattcttagt tccgtctggg ttagtgattg
    45961 ttactgtgtg gcttccatct ggattttcag tagttgttgc tgttgcagct ttaccgtctt
    46021 taccatcttt gatgatagcg ttggtaacat tgccgtctcc atctgtaact ttaacagtat
    46081 gagttccatc gttgttatcc gttacttcaa ctttaggagt cttaccgtct ttaccgtttt
    46141 taacaacagt ttctttagtt gaaccatctg gatttgtcac cgtaattgtg tggcttccat
    46201 ctgggttttc tgttgtagtg atattcgcag ttttaccgtc tttaccatct ttaacaactg
    46261 ttgttgattc agaaccatct gggttttgaa ctgtgatagt gtgagttcca tctccattat
    46321 cttttgtagt aatagttgga gctgaaccat cacgtccatc tataccgtta taaatatctt
    46381 tactatttaa tactttatcg acaccttcgt cgtatacccc attgtcatta acatcatcat
    46441 aaactgtaac acggataccg ttacgcgttg gagttgcagc agcatctgta tcgcgtctaa
    46501 ttcttgaagg tgaagtaata tcttctactt tgattttcgg tgcacggcca tctttaccat
    46561 tcttaccatc ttttacagtg aaggttactg gatctttacc tgatgttgtg atagttacaa
    46621 cagtgctttg gccatcttgt ccacgttgag tagtgattgt aggagtttgt ccatcctgac
    46681 cggttgcacc agtttctccc ttagcaccat cttgaacaaa gactgtagtt ggttgtgaac
    46741 catcatggtt atcaacagtg atttctacac ctttatgccc tgcttcatct ttacgaacag
    46801 ctgtaactgt aggtgatttt ccatctactc catctctaat taccgcaagt ggtttttctg
    46861 gatttaatgg ttgtcctggg ttagctggat cttcttggta aactttagtt tctttaccat
    46921 ccttgacagt aagcaatgat ttaccatctc gtccgtccgc gcctttagca ccatctttaa
    46981 tcatttctga acctagaagt tcatcagttc ctggtgtata tttgccgtca ttgttttcat
    47041 ctgtatagaa tgtcacaatt gtatgagatg gatttccgtt ttttccttgg acacgaagta
    47101 aatcaacttt tggagtttta ccgttcagac cattttctcc gtccttaaca tttactgtaa
    47161 ctggatcttt acctggaaca gtgaatgtaa tgtcagtact atggccatct ttacctggag
    47221 ttacagttac ttctggagtc ttaccatctt ctccgcgttc acctttgtca cccttagggc
    47281 ctttgatgtt gccctcttta tcccagttgc cgttattctt aacgaagacg tcgcctgttt
    47341 ctgtattaac gtatttatcg ccgtctttac cttgatgtgc ttctgggtta gctttgccgt
    47401 tcaacacgtc tttaccgtca cgtccatcac gacctactgc gccatccgca cctttagctc
    47461 cttgcgctcc agttaggccg cgttcgcctt ggtctccttt ttcgccttta gcaccatctg
    47521 cacctttgtc ccctttaggg cctttgatgt tgccttcttt atcccagttt ccattgtttt
    47581 taacatagac atcaccggtt tcggtattaa tatatttatc tccattttta ccttggtttg
    47641 gttgtggatt tacttttccg tttaatacat ctttaccatc acgacctgct tgtcctgtag
    47701 ctccatcacg gatcgtagtt gtcacatctg gacttcctgg attatgaacc gttaatgtat
    47761 gtgtgccgtt ttgcccctca gttattgtta ctgttggggt tttcccatca gcaccctttt
    47821 gtccatcctc tacaaatgtt gtttgtggac gacctcctac tggtgtaatt gttactaata
    47881 caccattttt gccattttgc acacttcgtg tcgctgtaat tgttggtgat tgtccattag
    47941 taccgtttct tccatcagtg atagttgcta atggttggtt tggattaagt ggttgtcctg
    48001 gtctagccgg atcctctaca tagacttttg tttcagttcc atttttaaca gcaattagag
    48061 attttccatc tttacctgga gttcctggat taccttgttc tccttttggt ccttgtggtc
    48121 ccgctggtcc tctttgtcca tctgcgcctt ttggtccttg agctccatca cgaccttgaa
    48181 gaccttgttc acctttgtca cctttggcac cttgcgctcc agttaaaccg cgttcacctt
    48241 gggctccttt ttcaccttta gatccagtta gaccgcgttc accctgatct cccttttcac
    48301 ccttgacacc ttgcgctcca gtcaggccgc gttctccttg ggctcctttt tcacccttgg
    48361 caccatctgc acctttgtca cctttcggtc ctttgatgtt tcctgctggt tcccaagtgt
    48421 tacctttctt aacgaataca tctcctgttt gtgtatttac gaatgtgtct ccatctttac
    48481 cttctgttgt tgggtctact tttccgttta atacttcttt tccatcacgt ccagatgctc
    48541 ctgcagctcc gtcttgtccg tctttaacta ctgttgtttg aactaactcg tcttttcctg
    48601 ctgtgtaact tccatctcca tctttgtcaa tgtagaatgt taatgttgtt tgtttcttcg
    48661 catcatctcg ttctgctttg acttttggtg tttttccatc ttcaccgttt ttaacgattg
    48721 ttgttaatga tggtctgttg tctggttgtg tgattgtgat ggtgtgtgtt ccgttattgt
    48781 tatctgtaac tgttacttct ggtgtgaatc catcttttcc gttatcacct ttgtcacctt
    48841 ttggtccttg ttttccatct gcgataacaa cttctttaag ttttgtatct ccttcatcaa
    48901 attgattatt atcatttaga tcataataga aaataacagt tgttgtttta tctgcttcat
    48961 ttctatgagt agaaacaaga gggcttgcac catcaaatcc atctcttact actgtagtag
    49021 ttataccacc tttaccatcg ttaattgtaa ttgtatgcgt tccatcttga ccatcagtta
    49081 ctttaactgt tggggttttc ccatcttctc cttttggtcc ttgttcacca tcacgccctt
    49141 gaagaccttg ttcacctttg tccccttttt cacctttagc accatctgca attacaactt
    49201 cttttaattt cttatcagaa gcatctaatt cattgtttcc atttttatcg taatagaaga
    49261 taacagttgt tgtcttatct gcgtcatttc gttgagtcgc aactaatgga cttgcgccat
    49321 cgaaaccatc tcttacaaca gtacttgtaa cattacctct accgtcgtta atagtgatag
    49381 tatgagtgcc gtcattttta ttatctttaa cggttacggt tggagttaca ccgtcacgac
    49441 ctgcctgacc ttgggctcct ttttcacctt tagctccagt taaaccacgt tcgcctcggt
    49501 ctcctttttc accttgggct ccagtcagac cgcgttcacc ttggtcccct ttttcacctt
    49561 tagctccagt taaaccgcgt tcaccttggg ctcctttttc acctttagct ccagtgatac
    49621 cacgttcgcc ttggtcccct ttttcacctt tagctccagt taaaccgcgt tcgcctcggt
    49681 ctcctttttc accttgagct ccagttagac cgcgttcgcc ttggtcccct ttttcacctt
    49741 tagcaccttg ggctccggtt agaccgcgtt caccttgggc tcctttttca cctttagcac
    49801 cttgggctcc agtcagaccg cgttcgcctc gggctccttt ttcaccttta gcaccttggg
    49861 ctccagtcag accgcgttcg cctcgggctc ctttttcacc tttagctcct tgagttccag
    49921 tcagaccgcg ttcgcctcgg tctccttttt cacctttagc tccttgagtt ccagttagac
    49981 cgcgttcacc ttgggctcct ttttcacctt tagcaccttg tgctccggtt agaccgcgtt
    50041 caccttggtc ccctttttca cctttggcac cttgtgctcc ggttagaccg cgttcacctt
    50101 gggctccttt ttcaccttta gctccttgag ctccagttag accgcgttcg cctcggtctc
    50161 ctttttcacc tttagctcca gttagaccgc gttcaccttg ggctcctttt tcacctttag
    50221 ctccatcttt aatagcaatt ttttgaacaa gttcgtctac accttcagta taaacaccgt
    50281 ttccatcgtt atcaagatag aaagtgaggt tatttgtatc accgacttta ttttgatcaa
    50341 cacgtggttt tactgtatcg attagatctt tagccacacc aatttgcata atacgtggct
    50401 tagcttcttt agtaacacta ttactaatcg gcaatcctaa aagtgtttcg ttaccaaatc
    50461 gatgaacttt ataggtaatt tgacgttgtc ctttttcacc ttctcgaact aatctagttt
    50521 gaccttctgg taacttacta gttggaagat cgacagtttg gaagtcaatg tcttctgaaa
    50581 cagtcttatt attagtacca ccataagggt ttgcatccaa gtatagtggg tttgctctat
    50641 aataatagtt agagttactg tatgggtcat tgctagtagc ggcaccacca ccaccttggt
    50701 ccacgttaaa agtaacaggt ttcaaacgaa cagacaaatc accatttaga ttaatgatag
    50761 gcaaactaaa tctgatagtt tgttccgtat ttgttgaaat agcagaggtc atactgtatc
    50821 ctgagtgaga tacgtagttg gcaccctgac taatagattt tgattgctcc cttactattg
    50881 gatttgaact tgtgatcgtt gcatttgaag gatttccaaa tccatcccct ttatccaaga
    50941 taaaattatt attaacaaac tctctagaat ctcctggcgc agaataacta attgtaaagt
    51001 taacactatt accactacgt actgttgtta catcgtaatg aatagaattt ccaccacgtg
    51061 agttcccgta gccacggaaa ctatagctac gaccaccttg gctaatatct gcaaatgcaa
    51121 ctgtatttcc agaacctgag acaattgggt tggctggatt gctagccgct ctaaaaccac
    51181 tataccctct attaacatct tccttacgga tagctggatt ttggcgagca tcagaaaggc
    51241 tcaaacctag tttcaagatt tcattaatag atgctggagc tgaagttgtt tctactttgt
    51301 cagctgtgct agttggcaaa atagcgctat caacagctga acgttcagtt gttgcagctg
    51361 gtttagcccc ttcttttgat tcaactgcat cagctttctt cgctacaggc gtttctgata
    51421 cagtttcttt attagagtta gaacgaagtt ctagaccaat aataaatgat tgaatagatg
    51481 actctgctgc tgataattca gcttcagttg catctgcttt tccaagcaag ctttgaccag
    51541 cttggatact tgtttgcaag ttagcagcac ttgcttctgt atataacttg ctttctaatt
    51601 ttttagcacg cgaaagagcc gttaataatt ttgacttgtc aacggcatct tgatgcgtta
    51661 cttcctcttt tttgtctttt gcttcttcaa ctttttctgc tggtttagta acttcttcag
    51721 ttttaactgg agtcgtttct actggattag ctacagttgg agctgcatag gttttctctt
    51781 tattagacgc agaaacctct gctggtttag tagctgcttc tcccctacca acagaaatac
    51841 tattggtagt ttcttgtttg ttttcttccg cttgtactgt ttggacacta gctgctgcac
    51901 ctaataccaa agcggtaccg agcaagacac tagctgcacc aaagtaatac ttacgaatag
    51961 aataacgttg ttgcatgcta taccagtcaa atgatttttt atgtgaatgt ttcattttaa
    52021 actcctagat agtattttat agtttacaat gtacaactat ttgaggcatt aatcaatatt
    52081 ttctgaacaa ttaatatatt ctgtttttgc acttaaaagc cacttatatt attctaattt
    52141 ttccgattac taacgacaat tttcttgctc taattttttt atactactta gacaaaaaaa
    52201 gatataatga attatggagg aaatacccgt atgagaaacc ttttatccac aaaagttcaa
    52261 agacaattac gcttaatgga aaccctaatt cagaatcgta attggatgaa attacatgaa
    52321 ctagccgaaa aattaggatg tacagaacga atcttaaaaa gtgatttaaa tgaattacgt
    52381 attgcctttc catctattaa catccaatct tctgttaacg gaatcatgat agatttagaa
    52441 gttaacacta gtgtagaaga tatttatcaa tactttcttg ctaattctca atcttttcaa
    52501 ttactagagt atatgttctt caatgagggg ctacctattt atcgaacaat agaaaatctt
    52561 tactttagta gtgccaacct ctatcgactg ggcagaaata ttacaaaggt tctttcatct
    52621 cagtttcaaa ttgaattatc ttttactcca tccgagatac gtggaaatga gatcgatatt
    52681 cgttactttt ttgcccaata cttttctgag cgctattatt tcctagattg gccttttcct
    52741 gaccttcctg aggaagattt aacagagttt gctgacttct tctataaaat cacaaattat
    52801 ccaatgcgat tttcaattta tagaatgtac aaactgatga tagctatcag tatccacagg
    52861 gttaaaaatg gtcacttcat tgacctcccc aaccactttt acaaagaata ttatcctctt
    52921 ttaaagagca ttccgaattt ccaggagaca cttgcttatt tctctaaaca ttttgggctt
    52981 gaaatgactc cagatacaat cgctcaaatt tttatttctt ttcttcagaa tgatattttc
    53041 ctagatcctc aagaattctt caactcttta gaagataaca gtcaagcaag atactcttac
    53101 caattactta gtcaaatatt agaaggccta tcaaaacaat ataagattac ttttactaac
    53161 cacgatgagc tgatttggca tttacacaac actgctttct ttgaaagaca ggaaatcttt
    53221 tctaccccaa tcttatttga acaaaaagca ttgacgatta aaaaatttga agtttacttc
    53281 ccagacttta tgggaagtgc gcgtcaagaa ttggctcaat accgtcaggc aattggacag
    53341 catgaccatc ctgaacagtt ggaacacttg atgtacacca tcttaaccca tgctgaaaac
    53401 ctctctactc agttgttaga aaatcgacca cctatcaagg ttttgattat cagtaacttt
    53461 gaccatgcta tatcccttac ctttgttgat atgctttcct attactgtaa taaccgcttt
    53521 accttcgata tttgggatga attaaaaaca agtcctgaga ttttaaatca gacagattac
    53581 gatatcatcg tgtctaattt ctatattcca gggattacca agaagtttat ttgccgaaat
    53641 catctgtcaa tcatgaattt ggttaatcat cttaacacct tatcaaatga gattcatttg
    53701 tccaatactt tataagttat caaaaaagtc agcataatcg tgctgacttt tc
//
LOCUS       DBEROU010000009        50872 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.009, whole genome shotgun sequence.
ACCESSION   DBEROU010000009 DBEROU010000000
VERSION     DBEROU010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 50872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.009"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            144..1163
                     /gene="galE"
                     /locus_tag="ACL8CL_000569"
     CDS             144..1163
                     /gene="galE"
                     /locus_tag="ACL8CL_000569"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGS1329868.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            1165..2118
                     /locus_tag="ACL8CL_000570"
     CDS             1165..2118
                     /locus_tag="ACL8CL_000570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1329869.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTSWSFRQLFFYSIE
                     GIVNFSDFPLTIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSDLPDFTEKIK"
     gene            complement(2180..2386)
                     /locus_tag="ACL8CL_000571"
     CDS             complement(2180..2386)
                     /locus_tag="ACL8CL_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HGS1329870.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDDPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            2438..2914
                     /locus_tag="ACL8CL_000572"
     CDS             2438..2914
                     /locus_tag="ACL8CL_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1386/EF1546 family surface-associated
                     protein"
                     /protein_id="HGS1329871.1"
                     /translation="MAKEPWQEDIYDQEESRAGHRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSEPESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            2923..3594
                     /gene="cmk"
                     /locus_tag="ACL8CL_000573"
     CDS             2923..3594
                     /gene="cmk"
                     /locus_tag="ACL8CL_000573"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HGS1329872.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            3725..4063
                     /locus_tag="ACL8CL_000574"
     CDS             3725..4063
                     /locus_tag="ACL8CL_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HGS1329873.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            4066..4695
                     /locus_tag="ACL8CL_000575"
     CDS             4066..4695
                     /locus_tag="ACL8CL_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HGS1329874.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            4935..6086
                     /locus_tag="ACL8CL_000576"
     CDS             4935..6086
                     /locus_tag="ACL8CL_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1329875.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            6399..7148
                     /gene="truA"
                     /locus_tag="ACL8CL_000577"
     CDS             6399..7148
                     /gene="truA"
                     /locus_tag="ACL8CL_000577"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HGS1329876.1"
                     /translation="MTRYKATISYDGYAFAGFQRQSHARSVQEEIEKTLTRLNKGQTN
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFIVDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGTHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            7138..7911
                     /locus_tag="ACL8CL_000578"
     CDS             7138..7911
                     /locus_tag="ACL8CL_000578"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HGS1329877.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LIKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            7889..8350
                     /locus_tag="ACL8CL_000579"
     CDS             7889..8350
                     /locus_tag="ACL8CL_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1329878.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
     gene            complement(8609..8857)
                     /locus_tag="ACL8CL_000580"
     CDS             complement(8609..8857)
                     /locus_tag="ACL8CL_000580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1329879.1"
                     /translation="MASTSLQAPAKICFILIKLYSPYAFLSKYLGAILLSFFCYYAKE
                     IGCNNLTLSVWNDNEGALRFYQRQGMKPQETTMEMIID"
     gene            complement(8805..9887)
                     /locus_tag="ACL8CL_000581"
     CDS             complement(8805..9887)
                     /locus_tag="ACL8CL_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HGS1329880.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFDLFTSTSKDLLYFD"
     gene            complement(10013..10795)
                     /gene="gatD"
                     /locus_tag="ACL8CL_000582"
     CDS             complement(10013..10795)
                     /gene="gatD"
                     /locus_tag="ACL8CL_000582"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit GatD"
                     /protein_id="HGS1329881.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(10795..12138)
                     /gene="murT"
                     /locus_tag="ACL8CL_000583"
     CDS             complement(10795..12138)
                     /gene="murT"
                     /locus_tag="ACL8CL_000583"
                     /EC_number="6.3.5.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_function: GO:0140282 - carbon-nitrogen ligase activity
                     on lipid II [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HGS1329882.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILEAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(12237..13553)
                     /locus_tag="ACL8CL_000584"
     CDS             complement(12237..13553)
                     /locus_tag="ACL8CL_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HGS1329883.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYLEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGTFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            13747..14973
                     /locus_tag="ACL8CL_000585"
     CDS             13747..14973
                     /locus_tag="ACL8CL_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HGS1329884.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPKVMGSLSAFLYAGGNILAGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSSSGKEKTASLTQGMAANQALKS
                     NRFYMLWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            15172..16746
                     /locus_tag="ACL8CL_000586"
     CDS             15172..16746
                     /locus_tag="ACL8CL_000586"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1329885.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQDIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
     gene            17010..17798
                     /gene="codY"
                     /locus_tag="ACL8CL_000587"
     CDS             17010..17798
                     /gene="codY"
                     /locus_tag="ACL8CL_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HGS1329886.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
     gene            17798..18373
                     /locus_tag="ACL8CL_000588"
     CDS             17798..18373
                     /locus_tag="ACL8CL_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HGS1329887.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            18455..18540
                     /locus_tag="ACL8CL_000589"
     tRNA            18455..18540
                     /locus_tag="ACL8CL_000589"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18489..18491,aa:Leu,seq:aag)
     gene            18823..19953
                     /locus_tag="ACL8CL_000590"
     CDS             18823..19953
                     /locus_tag="ACL8CL_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1329888.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(20100..20246)
                     /locus_tag="ACL8CL_000591"
     CDS             complement(20100..20246)
                     /locus_tag="ACL8CL_000591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329889.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            20454..21212
                     /locus_tag="ACL8CL_000592"
     CDS             20454..21212
                     /locus_tag="ACL8CL_000592"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1329890.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKALTGEMTLMKLSGMIYDEGLLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            21300..21812
                     /locus_tag="ACL8CL_000593"
     CDS             21300..21812
                     /locus_tag="ACL8CL_000593"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HGS1329891.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            21994..22938
                     /gene="metA"
                     /locus_tag="ACL8CL_000594"
     CDS             21994..22938
                     /gene="metA"
                     /locus_tag="ACL8CL_000594"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HGS1329892.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            22947..23624
                     /locus_tag="ACL8CL_000595"
     CDS             22947..23624
                     /locus_tag="ACL8CL_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HGS1329893.1"
                     /translation="MTYLDAFKSGTLVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTSQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFRN
                     AMDLWKD"
     gene            23722..24480
                     /gene="tpiA"
                     /locus_tag="ACL8CL_000596"
     CDS             23722..24480
                     /gene="tpiA"
                     /locus_tag="ACL8CL_000596"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HGS1329894.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            24518..26023
                     /gene="lytC"
                     /locus_tag="ACL8CL_000597"
     CDS             24518..26023
                     /gene="lytC"
                     /locus_tag="ACL8CL_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
                     /protein_id="HGS1329895.1"
                     /translation="MSVTFFIGEQRLKTKIGLASICLLGLATSHVAANETEVAKTSQD
                     TTTASSSSEQNQSSNKTQTSAEVQTNAAAHWDGDYYVKDDGSKAQSEWIFDNYYKAWF
                     YINSDGRYSQNEWHGNYYLKSGGYMAQNEWIYDSNYKSWFYLKSDGAYAHQEWQLIGN
                     KWYYFKKWGYMAKSQWQGSYFLNGQGAMMQNEWLYDPAYSAYFYLKSDGTYANQEWQK
                     VGGKWYYFKKWGYMARNEWQGNYYLTGSGAMATDEVIMDGARYIFAASGELKEKKDFN
                     VGWVHRDGKRYFFNNREEQVGTEHAKKIIDISEHNGRINDWKKVIDENEVDGVIVRLG
                     YSGKEDKELAHNIKELNRLGIPYGVYLYTYAENETDAENDAKQTIELIKKYNMNLSYP
                     IYYDVENWEYVNKSKRAPSDTDTWVKIINKYMDTMKQAGYQNVYVYSYRSLLQTRLKH
                     PDILKHVNWVAAYTNALEWENPYYSGEKGWQYTSSEYMKGIQGRVDVSVWY"
     gene            26520..27038
                     /locus_tag="ACL8CL_000598"
     CDS             26520..27038
                     /locus_tag="ACL8CL_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HGS1329896.1"
                     /translation="MVELKKEAAKDVATLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLAIYRYLITLFQKALDVTDEEGDDVTNDIFVGAKAELEKTVW
                     MLAAELGQAPGL"
     gene            27168..27674
                     /locus_tag="ACL8CL_000599"
     CDS             27168..27674
                     /locus_tag="ACL8CL_000599"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HGS1329897.1"
                     /translation="MTKKIVAIWAQDEEGVIGKDNRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEAYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            27674..27844
                     /locus_tag="ACL8CL_000600"
     CDS             27674..27844
                     /locus_tag="ACL8CL_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329898.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            27876..29108
                     /gene="clpX"
                     /locus_tag="ACL8CL_000601"
     CDS             27876..29108
                     /gene="clpX"
                     /locus_tag="ACL8CL_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HGS1329899.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            29117..29704
                     /gene="yihA"
                     /locus_tag="ACL8CL_000602"
     CDS             29117..29704
                     /gene="yihA"
                     /locus_tag="ACL8CL_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HGS1329900.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            29715..30095
                     /locus_tag="ACL8CL_000603"
     CDS             29715..30095
                     /locus_tag="ACL8CL_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HGS1329901.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            30147..31037
                     /gene="rapZ"
                     /locus_tag="ACL8CL_000604"
     CDS             30147..31037
                     /gene="rapZ"
                     /locus_tag="ACL8CL_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HGS1329902.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            31034..32011
                     /locus_tag="ACL8CL_000605"
     CDS             31034..32011
                     /locus_tag="ACL8CL_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HGS1329903.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDFRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            32008..32919
                     /gene="whiA"
                     /locus_tag="ACL8CL_000606"
     CDS             32008..32919
                     /gene="whiA"
                     /locus_tag="ACL8CL_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HGS1329904.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(32953..33921)
                     /locus_tag="ACL8CL_000607"
     CDS             complement(32953..33921)
                     /locus_tag="ACL8CL_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS1329905.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEEFAITTS
                     KGSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSA
                     VDWTLAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKL
                     EITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYA
                     IGDCCYYDGKIDLIATGLGEAPTAVNNAINYIYPEQKVQPKHSTSL"
     gene            34057..34914
                     /gene="cdaA"
                     /locus_tag="ACL8CL_000608"
     CDS             34057..34914
                     /gene="cdaA"
                     /locus_tag="ACL8CL_000608"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HGS1329906.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYMSTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            34901..35680
                     /locus_tag="ACL8CL_000609"
     CDS             34901..35680
                     /locus_tag="ACL8CL_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HGS1329907.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            35704..37056
                     /gene="glmM"
                     /locus_tag="ACL8CL_000610"
     CDS             35704..37056
                     /gene="glmM"
                     /locus_tag="ACL8CL_000610"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HGS1329908.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            37130..37504
                     /locus_tag="ACL8CL_000611"
     CDS             37130..37504
                     /locus_tag="ACL8CL_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HGS1329909.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            37506..38354
                     /locus_tag="ACL8CL_000612"
     CDS             37506..38354
                     /locus_tag="ACL8CL_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS1329910.1"
                     /translation="MKLAVFTDSSAYLSAETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGAGSIAL
                     GYIPVI"
     gene            38620..39387
                     /gene="dapB"
                     /locus_tag="ACL8CL_000613"
     CDS             38620..39387
                     /gene="dapB"
                     /locus_tag="ACL8CL_000613"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HGS1329911.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLTDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            39384..40583
                     /locus_tag="ACL8CL_000614"
     CDS             39384..40583
                     /locus_tag="ACL8CL_000614"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HGS1329912.1"
                     /translation="MRLTQMPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIH
                     DVDIATSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSA
                     VSFVRSLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDA
                     LRIMRGFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLA
                     SMIESQAYDYLPDMASSQNKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKS
                     WKTSRQFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQARGKEVNPQAI
                     TEKYQSLTIHDKKDIQINGDILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIH
                     AYLREKK"
     gene            40580..42451
                     /locus_tag="ACL8CL_000615"
     CDS             40580..42451
                     /locus_tag="ACL8CL_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS1329913.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFLKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYRGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKEVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKAEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIEALEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(42594..43778)
                     /gene="mntE"
                     /locus_tag="ACL8CL_000616"
     CDS             complement(42594..43778)
                     /gene="mntE"
                     /locus_tag="ACL8CL_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HGS1329914.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREETVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLENRF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            44082..46778
                     /locus_tag="ACL8CL_000617"
     CDS             44082..46778
                     /locus_tag="ACL8CL_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGS1329915.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDITPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            46791..47582
                     /gene="yghU"
                     /locus_tag="ACL8CL_000618"
     CDS             46791..47582
                     /gene="yghU"
                     /locus_tag="ACL8CL_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HGS1329916.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIANRPAVKRGLEVTYTEIK"
     gene            47698..48108
                     /locus_tag="ACL8CL_000619"
     CDS             47698..48108
                     /locus_tag="ACL8CL_000619"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGS1329917.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANRDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
     gene            48120..49715
                     /locus_tag="ACL8CL_000620"
     CDS             48120..49715
                     /locus_tag="ACL8CL_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329918.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPKSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVNDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTN
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESIKGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKVQ"
     gene            49882..50046
                     /locus_tag="ACL8CL_000621"
     CDS             49882..50046
                     /locus_tag="ACL8CL_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HGS1329919.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGIALV
                     LAAVWLGWPK"
     gene            50125..50853
                     /gene="yaaA"
                     /locus_tag="ACL8CL_000622"
     CDS             50125..50853
                     /gene="yaaA"
                     /locus_tag="ACL8CL_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HGS1329920.1"
                     /translation="MKILIPTAKEMNTDLPSIEAIPLKPESQTVLDALALYSASQLES
                     FYKVSAEKAAEEFQNIQALKRQTAQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
BASE COUNT        14811 a         9527 c        11604 g        14930 t
ORIGIN      
        1 cgacgttgac gtggtttgaa ttggattttc gaagagtata agcattttac tgaaatttta
       61 tcaggtagtt taggatggca aatgacattc cctgtcaaaa atggtaaaat aagaaaaaat
      121 aatctgagaa tcgaggaaaa aagatgcaag aaaagatttt ggtaactggt ggtgccggtt
      181 ttattggaac ccacactgtt attgaattga tccaagcagg ccatcaagtt gttgtggtgg
      241 ataatcttgt caatagcaat cgtaagagtt tagaagttgt tgaaagaatc acaggagttg
      301 agattccttt ctatgaggca gatattcgtg acacagctac cctcagagat attttcaagc
      361 aggaagaacc gactggtgtc attcactttg ctggtttgaa ggctgttggc gaatcaacac
      421 gtatccctct tgcctactat gacaacaata tcgctggaac tgtcagcctt ttgaaagcca
      481 tggaagaaaa caactgtaaa aacatcatct tcagttcttc tgcgacagtt tacggggatc
      541 cgcacacagt gcccatcttg gaagatttcc cactttcagt gaccaaccca tacggtcgta
      601 ctaagctcat gctagaggaa attttgactg atatttacaa agcagactca gaatggaatg
      661 ttgtcttgct tcgttacttt aacccaatcg gagcccatga gagtggtgat ttgggagaaa
      721 atccaaacgg tattccaaac aatctcttgc catatgtgac tcaagtagcc gttggaaaat
      781 tagagcaagt gcaagtgttt ggagacgatt acgatacgga agatggaaca ggtgttcgtg
      841 actatatcca cgttgtcgat ttggctaagg gtcacgttgc agctttgaaa aaaatccaaa
      901 aaggttcagg actaaacgtt tataaccttg gaactggtaa aggttactca gttcttgaaa
      961 ttatccaaaa catggaaaaa gcggtgggac gtcctattcc ttaccgcatc gtagaacgtc
     1021 gcccaggtga tatcgctgcc tgctactcag acccagcaaa agctaaagca gaactcggtt
     1081 gggaagcaga actcgacatc acccaaatgt gtgaagacgc atggcgttgg caaagcaagc
     1141 atccaaatgg atttgaagac taagatgatg atttcaatca tcgtcccttg tttaaacgaa
     1201 gaggaagtac ttcctctttt ttatcaggct ctggaagctt tacttccaga tttggaaaca
     1261 gaaatcgagt atgtctttgt cgatgatgga tcaagtgatg ggaccttgga actcttaaag
     1321 gcctatcggg agcaaaatcc ggcagtccat tatatttctt tctctcgaaa ttttggcaaa
     1381 gaagcagccc tttatgcagg cttgcaatat gcgacaggag atttggtggt ggtgatggat
     1441 gcagacctcc aagatcctcc tagtatgttg tttgagatga aaaatgtact agacaaaaat
     1501 gtagacttgg actgcgttgg gacacggaga actagtcggg agggagaacc cttctttcgc
     1561 agtttctgtg ctgttctctt ttatcgcctc atgcaaaaaa tcagcccagt agctctgccg
     1621 tcgggtgtcc gtgattttcg tatgatgaga aggtctgtag tcgatgccat tttaagcttg
     1681 accgagtcca atcgtttttc aaaggggctc ttcgcctggg tgggtttcaa aacctactat
     1741 ttggactatc caaatgtcga aaggcaggct ggcaagacca gttggagttt taggcaactc
     1801 tttttttact ccattgaagg gattgttaat ttttcagatt tccctttgac tatagccttt
     1861 gtagctggtc tcctatcttg ttttctttct ctgctgatga ccttttttgt tgtggttcgg
     1921 accctcattt tgggcaatcc gacatctggt tggacctctc tgatggctgt tattctcttt
     1981 cttggaggca ttcaactctt gaccattggg attctcggta agtatatcag taagatttat
     2041 ttagagacta aaaaaagacc actttatctt atcaaagaaa aaagtgacct tcctgatttt
     2101 acagaaaaaa taaagtgaaa agactataat tttacatgga aatgtgctaa actagaggga
     2161 gtggattatc gccattgatt taggcttcct ggtttccaat cagggcgcga gttgggcaat
     2221 ttttaacagc ctctaagaca tcctgactag gagaaatttc tttttccagt tggtcagggt
     2281 catcgtaaaa acgcacgatt ccattatcgt ggtaatcaaa taaatcagaa taagtttggc
     2341 aaagtccaca ggcgatacat cgttcaggta taagtgtgat tttcatgttc atatttatat
     2401 tgtaataaga aaagtaaaaa aaaacaagga gtaaggtatg gcaaaagaac cgtggcaaga
     2461 agatatttat gatcaagaag aatcaagagc agggcatcgg catcgaaacc acggaggggc
     2521 tgataggatg gctaatcgta ttttgacgat cctagctagt attttctttg taattgtggt
     2581 ggtgatggtc atcgttctca tctatctatc atcggggggg agtaatcgca cagcagcctt
     2641 aaaagacttt catgattctg atgcaagtgt agtacaaatc tcatcttcaa gcagttctca
     2701 gcctgagcag agttcagagc cagaatctac ttctagtagt tcagaagaag ctgctaatcc
     2761 tgaaggaacg attaaagttc tcgcaggaga aggggaagca gctattgccg ctcgtgcagg
     2821 aatctccatt gctcagttag aggccttgaa tcctgggcac atggctacag gatcttggtt
     2881 tgctaatcca ggtgatgtta taaaaataaa ataggagccg atatgaaaac aattcaaatt
     2941 gctattgatg gtcctgcttc cagcggtaag agtacggtcg caaagattat tgctaaggat
     3001 tttgggttca cctaccttga tacaggagct atgtatcgtg cagcgaccta tatggctctt
     3061 aagaaccaat taggagttga agaagtcgaa gcccttctag ccttgttgga ccagcatcca
     3121 atcagctttg gacgttcaga aactggagac cagcttgttt ttgtaggaga tgtggatatt
     3181 acccatccta tccgtgaaaa tgaagtgacc aatcatgttt ctgctattgc agcaattcct
     3241 gaagtgcgtg agaaactggt ttctctccaa caagaaattg cccagcaagg cgggattgtc
     3301 atggatggtc gcgatattgg aactgttgta ttgccacaag cagaattgaa aattttccta
     3361 gtagcttctg ttgatgagag agcagagcgt cgttacaagg aaaatattgc caagggaatt
     3421 gaaacagacc ttgaaaccct aaaaaaggaa attgctgcgc gtgactacaa ggatagtcat
     3481 cgtgagactt ctcctctcaa acaagcagag gatgctgtct accttgatac aactggtttg
     3541 aacattcaag aagtagttga aaaaatcaaa gcagaagctg aaaaaagaat gtaaatgtag
     3601 aaaagccgat agattgatat cggttttttt ctaaatttgt caaaatgctg attttagggt
     3661 ataatagttg ctgttcgaga aattttgatt ttcaaagaat agtgaatgat gataaggaga
     3721 aaccatgaac aatttaccaa attgtccaaa atgtaactca gagtatgtct acgaagacgg
     3781 tgctctactg gtttgcccag agtgtgctca tgagtggaat cctgctgaag ttgcagaagt
     3841 agaagaaggt cttgtcgcta tcgatgccaa cggaaataaa ttgactgatg gtgatacagt
     3901 aactctcatc aaggacttga aagtaaaagg tgcgccaaaa gatttgaaac aagggacgcg
     3961 cgtgaaaaat atccgcatcg tagaaggcga tcacaatatc gactgtaaga ttgatggttt
     4021 tggtgccatg aaacttaaat cagagtttgt gaggaagatt taagcatgtc aaaacactat
     4081 aaacttgtat tttatagccg tatcttcttg tttctagcgg cttttacggg agtttatctt
     4141 gaaattacta ggcatggtgg ttttgggatg cttctctatt acacggttct gtccaacctc
     4201 ttggtaacta tttttaccct ttatcttcta aaggttatga gccgtgtagg tgaaaattgg
     4261 caaaggccaa gtctcttgcg cttaaaaggt ggggtcacca tgagtatcat gattacctgt
     4321 gtgatttacc atttcctctt ggcgcccatt gcgactaatt tctataccct agaaaatttc
     4381 ctttgccact atatcgttcc catctggttt ttagcggata ccctcttttt tgacaaacag
     4441 ggtcaataca agatttggga tccagcagtg tggaccattt taccctttct gtatatgatg
     4501 tttgctcttt ttaatggctt ggttctaaaa ctcaatattc caaatgccaa ggataatcct
     4561 ttcccttact tctttttgaa tgtgaacaag ggttggaatg ttgtgtttaa gtggtgtctg
     4621 attatctttg ttgcctatat ggtagcagga tttattttct actttatcaa gcaaatcaag
     4681 agaaagtcat cctaatactc ttcgaaaatc tcttcaaacc acgtcagttt ccatctgcaa
     4741 cctcaaaaca ctgttttgaa caacctgcgg ctagcttcct agtttgctct ttgattttca
     4801 ttgagtataa gatacaaggg attcattttc gaatttaaga aggagtctac aaaccaatga
     4861 ggcttcttct tttcttgctt atttgataac tatcaaaaaa gtagaagatt aagagaaata
     4921 tagaaagaga tttcatgaaa caatttttag aacgggccag cattttggct ctctccctcg
     4981 ttttgattac ctccttttcc atttcgagtg ccctaccagc catgtttgac tattatcagg
     5041 gttattctaa ggaacaaatt gagctcttgg tgagcttgcc ttcctttgga atcatgatga
     5101 tgttactgct aaatggtttc ttagaaaaaa tatttcctga gcgcttacag attagtttgg
     5161 gcttgctgat tttatcattg agcggtacag ctcccttctg gtaccaagcc tatccctttg
     5221 tctttggaac acggcttctc tttggtttgg gtcttgggat gatcaatgcc aaggccattt
     5281 ctattatcag tgaacgctac caaggaaaaa ggcgaattca gatgttaggg ctacgcgctt
     5341 ctgcagaggt cgttggagct tctctcatta ccttggccgt cggtcagttg ttggcctttg
     5401 gttggacagc tatctttcta gcctatagtg ctggattttt ggtgctgccc ctttatctgc
     5461 tctttgtccc ttatggaaaa tcaaagaaag aagtcaagaa aagagcgaag gaagcaagtc
     5521 gtttaactcg agaaatgaaa ggcttgattt ttaccttagc tatcgaagcg gcagttgtag
     5581 tttgtaccaa tacagctatt accatccgta ttccaagttt gatggtggaa agaggattgg
     5641 gggatgccca gttatctagt tttgttctta gtatcatgca gttgatcggg attgtggctg
     5701 gggtgagttt ttctttcttg atttctatct ttaaagagaa actgctcctc tggtctggta
     5761 ttacctttgg cttggggcaa attgtgattg ccttgtcttc atccttgtgg gtggtagtag
     5821 caggaagtgt tctggctgga tttgcctata gtgtagtctt gacgacggtc tttcaacttg
     5881 tctctgaacg aattccagct aaactcctca atcaagcaac ttcatttgct gtattaggct
     5941 gtagtttcgg agcctttacg accccatttg ttctaggtgc aattggctta ctaactcaca
     6001 atgggatgtt ggtctttagt atcttaggag gttggttgat tgtaatctct atctttgtca
     6061 tgtacctact tcagaagaga gcttaggatt gattcctaag tttttctttt gatactcaat
     6121 gaaaatcaaa gagcatagtt gattgagttt ggaatagtac gctgtagttg ctaaaatatt
     6181 tctagaaatt aatttgactt ttctaataga gttattcata tcttatttca atctactata
     6241 gaaagctagc cgcaggttgc tcaaaatagt gttttgaggt tgcagatgga aactgacgtg
     6301 gtttgaagag attttcgaag agtatgagat ttttataccc atacaatatt tgttttaggt
     6361 ccttgtttac agactaattt cttgataaaa tagaggttat gacgagatat aaagcaacta
     6421 tttcctatga tggttatgcc tttgctggct ttcagcgcca gtctcatgcg cgtagcgttc
     6481 aggaagaaat tgaaaaaacc ttgaccagat taaataaagg gcaaaccaat actgttcacg
     6541 gtgctggtag gacagatagt ggggttcatg ccctgggaca ggtcattcat tttgacctgc
     6601 cttatcagat ggatgaggag aaactccgtt ttgccttgga tacccagtct cctgaagata
     6661 ttgatgtgat ttcgattgag cttgtggcag atgattttca ttgccgttat gccaaacata
     6721 gcaagaccta tgagtttatt gtggatagag gacgtcccaa aaatcctatg cgccgtcact
     6781 atgccaccca ctttccctac ccactcgatg tggaacgaat gcagattgca atcaaaaagc
     6841 tagagggaac ccatgatttt accggtttta cagcctctgg gactagtgta gaggataagg
     6901 ttcgcaccat cacagaagct agtttaatag tcgatgagac aggacaattt ttgaccttta
     6961 ccttttcagg aaatggtttc ttgtataaac agattcgcaa tatggtgggg actcttctca
     7021 aaatcggtaa caaccgcatg ccagtagagc agattgatct catcttggag aagaaggaca
     7081 ggcaacttgc aggtcccact gcagcaccaa atggtttgta tttaaaggag attcgttatg
     7141 aagaataatc gtattttagc actttctgga aatgatattt ttagtggtgg tggactgtca
     7201 gctgatttgg ctacctatac cttgaacggc ttgcatgggt ttgtagcagt gacttgtttg
     7261 acagccttga cagaaaaagg atttgaagtc tttccaactg atgataccat ttttcaacat
     7321 gaattagata gcttgcgtga tgtggaattt gggggaatta agattggtct tctccctact
     7381 gtcagtgtgg ctgagaaggc cttggacttt atcaaacaac gcccaggagt acctgtggtg
     7441 ttggatcctg tcttggtctg caaggaaacg catgatgtag ctgtcagtga gctctgccaa
     7501 gagttgattc gctttttccc ttatgtcagt gtgattacgc ctaatctccc agaagcagaa
     7561 ttattatccg gtcaggaaat taaaaccttg gaagacatga aaactgcagc gcagaaattg
     7621 catgatttag gagcgccagc agtcattatc aagggaggca atcgtcttag tcaggacaag
     7681 gctgtggatg tcttttatga tggacagacc tttactatcc tagaaaatcc agttatccaa
     7741 ggccaaaatg ctggtgcagg ttgtaccttt gcctctagca ttgccagtca cttgattaaa
     7801 ggtgataaac ttttgccagc agtagaaagc tctaaggctt tcgtttatcg tgctattgca
     7861 caagcagatc agtatggagt aagacaatat gaagcaaaca aaaacaacta aaatcgccct
     7921 tgtatcccta ttaaccgccc tttctgtggt tctaggttat ttcttaaaaa tcccaacacc
     7981 tacaggaatt ctaactcttt tagatgctgg tgtcttcttt gcggcctttt actttggtag
     8041 tcgtgaagga gcggtagtcg gaggactagc aagtttcttg attgacctct tatcaggcta
     8101 ccctcagtgg atgttcttta gcttggtcaa ccatggcttg cagggatttt tcgcaggatt
     8161 taaaggaaaa agtcagtggt taggccttat tttagcaact attgccatgg taggaggcta
     8221 cgccttgggt tctactttga tgaatggctg ggcagcagcc ctcccagaaa ttctaccaaa
     8281 tttcatgcaa aatatggtag ggatgattgt aggatttatt cttagtcaaa gtatcaagaa
     8341 gattaagtaa tactcttcga aaatctcttc aaaccacgtc agtttccatc tgcaacctca
     8401 aaacactgtt ttgagcagcc tgcggctagc tttctagtct gattttcatt gagcataaag
     8461 aggttgagta aaaagtcttt caaataagaa aaacgcatag tatcaggtgt tgaatgaaca
     8521 cctgatacta tgcgttttat tgtgggaaga ttttcctcat tttctagtga aattgaactt
     8581 tttctaacat cttttgatag atgacttctt aatcaattat catttccatt gttgtttctt
     8641 ggggtttcat cccttgtctt tgataaaagc ggagagcacc ctcgttatca ttccagacac
     8701 ttagggttag attattgcag ccaatttctt tagcataata gcagaaaaat gacaataaaa
     8761 tagcccctag atattttgaa aggaaagcat aggggctata tagtttaatc aaaataaagc
     8821 aaatctttgc tggtgcttgt aaagaggtcg aagccatcct tggtaacaac accgcagtct
     8881 tcaatacgaa caccgacttt accagggata tagatacctg gttcaacaga gaagcacatg
     8941 ccttcttcga tgaccatgtc gtttccttcc atgatagatg ggaattcatg gacatccata
     9001 ccgataccat gcccgagacg gtggttgaag tactcaccat aaccagcttt ttcgatgacc
     9061 tcacgggcag cgcggtccac ttcatgagca gtcacacctg gcttgataaa gtcaagagca
     9121 gcttgttggg cttcaagagt caagttgtaa atatctttct tgaattggtc tggtttgccg
     9181 acagcgactg tacgagtcat atctgacgca tagccattga ccagaacacc caggtcaaag
     9241 aggagaagag catcattttc aaccttatta gctgctggaa tgccgtgtgg attcgcagca
     9301 ttatcaccag tcaagaccat ggtatcaaag ctcatttcgt aaccttcacg tttcatggca
     9361 aagtcaattt gtgcgatgat atctgtctca gtcttatcaa gagaaatatt gtcaaaacca
     9421 acatgaacag ccttgtcagc ataaagacct gcaaccatca ttttttgcac ttcatcagct
     9481 gatttgatga ggcgcatgcg ttggatacga ggagtgaggt tgtcaaactc agcagtctca
     9541 aaaactgttt tcaaaccatg gtatttggtc aagatgagat tgtcaaactc aacagcgaca
     9601 cgtttgaagt caagttgtgg aagagcatgt ttgatttttt gccatggatt ttcagaatcg
     9661 acatagccca ctactgggaa ggaaacggtg ctacttgcac gttctacttc aagagctggg
     9721 acaaagagga gaggttcctg atctgctagg acaaagagga acatttggcg ttcatgggga
     9781 tcactgtaaa aaccagtgag gtaattgatt gtgacggggt cagatacgac agcgacgtct
     9841 agtttttctg attcaagata tgttaggatt tgttgtaatt tagacatatg ctaccttctt
     9901 tctatccctc tattttggca aaaaatgaga gaaaatgcaa gggagaaggg taaaagaaaa
     9961 gacaaaaaac tccgacaaga taacggagtt ctttgatatc attttccttt gtttaagaaa
    10021 agtcagcctt gcttttgacg tcactgtact cttcagcgat ttcttggctg aggatatcct
    10081 cataggcagg gagttggata tcctgaccat atttcttctt gagggcagta gtaactaggc
    10141 gataagccag attggcatta cgagagagga taggcccgtg gaagtaggaa ccaaagacat
    10201 tcttataatg aaccccttca ccgacctttt cttcgttgtt tccatttcca tagacaacct
    10261 gtcccagcgg tttttggtca tcagagagga aggtacggcc ttggtgattt tcaaatccat
    10321 agtaggtttc atcgaaatct tcattgtgaa tcttgatgtc accgataaaa cggttattgg
    10381 tctggttgag cgtgtagtgt cccatgaccc ctagcccttc gatacgtttt cctgaagctt
    10441 caacataata ttgacccaat agttggaaac caccgcagat agccagaact acaccgtcgt
    10501 tttggatgta gttgtcaatg ctctcttttt tagcaggtag gtcgtctgca atgatacttt
    10561 gttcaaagtc ttgaccacca ccgaaaaagg cgatgtcgta gtgattttca tcaaagtcat
    10621 catggagaga aacgatgtca acggtcacat gggctcccag tttttcagcc acatacttga
    10681 gcatgaggat gtttccattg tccccgtagg tattcatgag atttccgtag aggtgggcaa
    10741 tgttgagctg ataggggtaa ttgccatctt ttgaggaaag tgaagtataa accattagtt
    10801 catctccttt ctaacaatct gacgactagc cagcagttca cgaaattcca gcatggcagt
    10861 ataagttgcc agaatatagg catgcttgca gtcttgattc tcaatggtct tgagaacttg
    10921 ctccagatta ctcgtttcag tgattttctc agctggatag ccagtcactc ggaggcgacg
    10981 agcgatttca gaatgacgaa caccgccagc gttgatttca ggaatgtcca tgtcagtgat
    11041 ttgttcaaag tctgcatccc agatccagct agtgtcaatt ccatctgcat agttggcatt
    11101 aaggaggaca gataggctaa atggataagg tgctagtttg atcatttcga tagcttgggt
    11161 tgcaccgact ggatttttaa tcaagacaag ggtacattcc ttgtcaccga tatgaaaggt
    11221 ttcttggcgt ccaaagacag cacggctctt gtcaaatccc tgtttgatga gttgcgaatc
    11281 ggcacctagg aaacgggcga tggccacagc agctagggcg ttatagatat tatagagccc
    11341 gccgatttgg ataccgtatt cttggccgtc tatgacaaag cgagagcgat tgttggtcaa
    11401 ctcaaccagt tttgtcaaac gatagtcgag atcaggacgt ttgcatccac aaccttcaca
    11461 gatataggca cccaagtttg cataggtatt atgctcatat ttgaggatgc cttggcagtc
    11521 aggacagaga atcccttcgg tattgtagtg agccagttgg gctggtcctt tttccaagtc
    11581 aaaaccaaaa tactctatag ggtttggaat agttggcttg tagaaaagtg gactgtctcc
    11641 gttaaggaga acagtagcag ttggaacttt ccgaatggct tccaatatca tgttataggt
    11701 agtatagatt tcaccgaaac ggtccatctg gtcacggaag atattagtaa tgacaaaaag
    11761 actaggctgg atatagtcac agatacgaga tagactggct tcgtcaattt cgaggacggc
    11821 aatatttttc ccagtttttg aagatttggc tgttaggaag gttgttgcaa tccctgtaat
    11881 catgttggca ccgcttgggt tggttagaac ttgaccataa acctctttta aaatgccgac
    11941 agtgagggca gttgtcaggg tttttccatt tgttccagtg acaacgacaa tctcgtagtt
    12001 cttagctagg ttttgtaaaa tatctttatc aaattgaagg gcgactttac ctgggagcgt
    12061 acttccacgt ccaagacggc ttaaaacgaa gtgggaagaa cgcccagcaa gaaggcccaa
    12121 agtagttttt aagttcatgt ttctattata tcacaaaaga gggaaaagac agatttggtg
    12181 tttcgtagaa gtaaaaacag cccaaagagg gctgtcagtt aagatgagat ttcaacttaa
    12241 atcgcaaaca agtcattcaa gttctcagcc aaggttgggt gagtgaagat ttgttttgtg
    12301 aagtaagtgt aaggaatctt gttgtccata gcaacagtaa tgatgttgat gatttcttga
    12361 gaaccttctg agaagatgct tgcaccaaga atttcttttg tttcagtatt aacaacagct
    12421 ttgaaagttc cgcgaaggtc tccatttacg tgaccacgag gcatggctgc aacagggatt
    12481 tctttcactg cgtatggaag tttcaaatca gctgcttggc tttcagtcaa accaacttgt
    12541 gaaagtgcag gtgtgatgaa catagtattt ggcacattga gacggtcctc aagtgtgtag
    12601 ctgccatctc cagcaaggta gctgtaaaca acacggaagt catcaagtga aatgtaagta
    12661 aattgaagac caccgttgac atctccaact gcaaagacac caggaacgtt tgtttgacag
    12721 tgtttatcta ctttaatagc gccacgttca gttagttcaa tatctgtatt ttcaagttga
    12781 agtggttcta catttggttt gcgtccagtt gcgtagagaa gggcgtcgaa acggtaagtt
    12841 tcgtcttcag ttacgacaag cacttggtca ccgtcgtttt taatttcagt agtatggata
    12901 ttttgaagca attcaatacc gtcttcttcc aggtattgtt tagcaagagc tgcgatggaa
    12961 ggttctgcac gaggtaggaa tgtatccaag gcatctagga ctgtaacctt gcttcctagt
    13021 ttattgtaaa ggccagcaaa ttcaagaccg atatttccgc caccaaggac tccaagtttt
    13081 tcaggcaatt tatccaagct ttggatacct gttgagtcaa agacgttttt gcttgtagca
    13141 agtccaggga ttggcaagac gtttgaaaca gcaccagtgt tgatgacgat agtttcagca
    13201 gtcaattctt gtttttcatc accagcttgg atttcgatga ctttatttga aaggaagtga
    13261 gcttcagcat caaagatatc gacgccagta ccagcaacag tcgtatagtt tttaccgttg
    13321 aggcgaccag tgatcgtgtt tttggtagca atgacttctt caaaagacaa gtctttctca
    13381 gcagcaacca gcaaagtttt agttgggata caaccgatgt tgatacaagt tccaccgtac
    13441 atagctttgc tacgttcaac gagggcaact tttttgccag ctgaagccaa tttaccagct
    13501 agtgttttac cagctttacc aaatccgata acgattaaat cataagttaa catttagaat
    13561 tcctcctatt cgaattacat tctagcacaa gaaaaaaact tttgcacaat tctgctacga
    13621 aaaagacaga aaaatatgaa aacgctttcc aggcaaaaaa ctagtaattt tttgacaagc
    13681 ttgataaact aaaatcctat ctatttttat ataagataga ataaatgttc gaaaatagga
    13741 ggttttatga aatcgaatcg ttatattatt gcctttgctg gggtcatttt acacttaatg
    13801 ctgggttcga cttatgcctg gagtgtttat cgtaacccta tcattgaaaa aacgggatgg
    13861 gatcaggctt ctgttgcctt cgcctttagt ctagcaatct tttgtttggg cttatcggct
    13921 gcatttatgg ggcgtttggt agaaaaattt ggtccgaaag tcatgggaag tctatctgct
    13981 tttctatacg caggtggaaa tatcttagca ggatttgcaa tagaccgtca ggagctgtgg
    14041 ttgttgtatc tcgcttatgg cattttaggt gggcttggtt tgggagcagg ctatattacc
    14101 cctgtgtcga cgattataaa atggtttcct gataaacgtg gtctcgcaac aggtttagcg
    14161 attatggggt ttggttttgc ttctttattg actagtccca tagcgcaaca cctcatcgca
    14221 ggggtagggc ttgtagaaac tttttatatt ttaggagcaa gttactttat tatcatgctc
    14281 ctagcttcac aattcattaa gcgtccaaat gagcaagagc ttgcaatttt atcttcttca
    14341 gggaaagaaa aaacagcctc tttgacgcaa ggaatggctg caaatcaggc tctgaaaagc
    14401 aatcggtttt atatgctttg gattattttc tttatcaaca tagcttgtgg tttaggctta
    14461 atttcagcgg catcgccaat ggcacaggag atggctggct tgtctacaag tcatgcagca
    14521 gtaatggtgg gtgttttggg gattttcaat ggatttggtc gcttgctctg ggcgagtttg
    14581 tctgactata tcggtcgccc tctaaccttt agtatattac tgcttgtcaa tcttttcttt
    14641 tctctctcac tttggctctt tacagattcc gttttatttg tagttgctat gtctattttg
    14701 atgacttgct atggagctgg tttttctttg attccagctt atctcagtga tatttttgga
    14761 accaaggaat tggccgctct gcatggttat attttaacag cttgggcaat ggctggttta
    14821 gcgggaccta ttttattagc agagacttat aaaatggctc attcgtacac acaaaccttg
    14881 ttcgtttttc tcattttata cagtatcgcc ttggctttgt cttattatct aggtcgttca
    14941 atcaaaaaag aaagtcaaaa accgcttaca tgattttctg tagaaatggg gctatctttt
    15001 gctctggtct tgtgttatac tagataggtt gcaaagaaaa cagtactttt cttttgtgga
    15061 aaagaagcaa cacgatttta tatctagtta tatgaaaata cgtcataaaa agaaaagtat
    15121 aaactaagcc ctatagggtg gcttcgcacc acctttagaa agaagaataa cgtgaaattt
    15181 aatgaattaa acttgtctgc tgatttgcta gcagaaattg aaaaagctgg ttttgtagaa
    15241 gctagtccta tccaagaaca aactattcct ttggcccttg aaggcaagga tgttatcggt
    15301 caagctcaga ctggtacagg aaaaactgca gcctttggct tgcctaccct tgaaaaaatc
    15361 cgtacagaag aagcgactat ccaagccttg gtcatcgctc caactcgtga actagctgtc
    15421 caaagtcaag aagaactctt ccgctttggt cgtagtaagg gagtcaaagt ccgttcagta
    15481 tatggcggat caagcattga aaaacaaatt aaggctctta aatctggtgc ccatatcgtg
    15541 gtgggaactc caggtcgcct cttggacttg attaaacgca aggccttgaa attacaagac
    15601 attgaaaccc ttatccttga cgaagcggat gaaatgctta acatgggctt ccttgaagac
    15661 atcgaagcca ttatttcccg tgtacctgag aaccgtcaaa ctttgctttt ctcagcaact
    15721 atgccagatg ccatcaaacg tatcggtgtt cagtttatga aagcccctga acatgtcaag
    15781 attgcggcta aggaattgac aacagaattg gttgaccagt actatatccg tgttaaggaa
    15841 caagaaaaat ttgacaccat gactcgtctc atggatgtgg cacaaccaga actcgctatt
    15901 gtatttggtc gtaccaaacg ccgtgtggat gaattgactc gtggtttgaa aattcgtggc
    15961 ttccgtgcag aaggaattca tggcgaccta gaccaaaaca aacgtcttcg tgtccttcgt
    16021 gactttaaaa atggcaatct tgatgttttg gttgcgacag acgttgcagc gcgtggtttg
    16081 gatatttcag gtgtgaccca tgtctacaac tacgatattc cacaagatcc tgagagttat
    16141 gttcaccgta tcggtcgtac aggtcgtgct ggtaagtcgg gtcaatctat tacttttgtt
    16201 gctccaaacg aaatgggtta ccttcaaatc attgaaaact tgactaagaa acgcatgaaa
    16261 ggtctcaaac ctgcaagtgt agaagaatcc ttccaatcaa aaaaacaggt agctctcaag
    16321 aaaatcgaac gtgattttgc agatgaaacc attcgtgcca actttgagaa atttggtaag
    16381 gatgctcgca aattggctgc tgagtttact ccagaagaat tggcaatgta tatcttgagt
    16441 ctgacagtcc aagacccaga tagccttcca gaagtggaga ttgcacgtga aaaaccacta
    16501 ccgtttaaac catcaggtaa tggtttcggt ggtaaagcta agggaggtcg tggaggccgt
    16561 cgtggggacg accgtcgaga gcgtgatcgc cgtggcaatg gtcgccgtga tgagttcaaa
    16621 aaaggaagtc gtggcaacga tcgttttgat aaggaaaaac gttaccgtaa ggataataaa
    16681 aaaccacgca atactttaag cgaaaagcaa acaggctttg ttattcgtaa caaaggcgat
    16741 aaataagaaa aagcggttca aaatgaatcg cttttttata taaggagacc gaatgtgaaa
    16801 agacaaataa aattattata ttatcttttg taatgttatg atacacaaaa ataggcaact
    16861 tgtcaataga aaaggaacaa tttaattaaa aatattctgg tttttaatta aattgtaagt
    16921 caatgcaagt caataaggaa ttttcagata attattgaaa aagagacgtt tttatgttat
    16981 aatgatagcg attaaattcg aggtgaaaaa tggcacattt attagaaaaa actagaaaaa
    17041 ttacatcaat tttgaaacgc tcagaggagc agttgcagga tgagctccct tacaacgcta
    17101 ttacgcgtca gttagcggat attattcatt gcaatgcctg cattatcaat agtaagggac
    17161 gtctgcttgg ctattttatg cgttataaaa caaatacaga tcgcgtagag caattcttcc
    17221 aaactaagat tttcccagat gactacgttc aaggggctaa tatgatttac gaaacagaag
    17281 caaacttgcc tgttgagcat gatatgagta ttttccctgt tgagagtaga gatgattttc
    17341 cagatggctt gacgactatt gcaccgattc atgtatcggg gattcgcctt ggttctttga
    17401 ttatttggcg taatgataaa aaattcgaag atgaggactt ggttcttgtt gagattgcca
    17461 gtaccgttgt tgggattcag cttcttaact tccaacgtga agaagatgag aaaaatattc
    17521 gtcgtcgtac tgctgtcacc atggcggtca ataccctttc ttactccgaa ctccgtgctg
    17581 tttcagcaat tttaggggaa ttaaatggaa atgaagggaa gttgactgcg tcagtgattg
    17641 cagatcgtat cggaatcact cgctctgtga ttgtcaatgc tcttcgtaaa cttgagtctg
    17701 cggggattat tgaaagtcgc tcacttggaa tgaagggaac ctatcttaag gtcttgattt
    17761 cagatatttt tgaagaagtg aagaaaagag attactaatg acaaaggctt taatttcgat
    17821 tgattataca gaagattttg ttgctgatag tgggaagctg acagcaggtg ctccagctca
    17881 ggcgatttcg gatgccatta gcaaggtaac tagattagct tttgaacgag gagattatat
    17941 cttctttact attgatgctc atgaagaaaa cgattgtttc catccggaaa gtaagttatt
    18001 tcctcctcat aatctgattg ggacgagtgg acggaattta tatggagatt tggggatctt
    18061 ttatcaagag catggttcag acagtcgtgt cttttggatg gataaacgcc attactcagc
    18121 tttttcaggg actgacctag atattcgttt gagagagcgt agagtgtcta ccgttatctt
    18181 aacaggtgtc ttgacggata tctgtgtcct acatacagct atagatgcct ataatctagg
    18241 atatgacatc gagattgtta aaccagctgt tgcttccatc tggcctgaaa atcatcaatt
    18301 tgccctaggt catttcaaaa atacactcgg agctaagtta gtagatgaaa atctaaatga
    18361 actttctgag taacctggtt ggtgaaaatg aaaaaatttg aaaaaaagtg ttgacaagca
    18421 tcccaaaagt tgatatacta gtaaagtaat cgacgcgggg atggcggaat tggcagacgc
    18481 gcaggactaa ggatcctgtg accgctttag gtcgtgaggg ttcaagtccc tctctccgca
    18541 taggaaaaga gttagctaaa gctagctctt ttgtatttct ctaaaaaatt agaatccagt
    18601 caaatgtagt ggagaatcag cgaaaatatg ggaaaactga tccttgtttc tgcttttgtc
    18661 atagaagaat aataaagatt atcttcttca aattctccga tattctctaa agttttgtgc
    18721 aagttgcaca gaactttttt atttttttgg tcatcttgcc atagaaatat aaagcgtttt
    18781 catctataat ataattatca aaagacaaaa ggagttcacc tcatggtaga attgaatctt
    18841 aaaaatattt acaaaaaata tccaaacagc gaacactatt cagttgaaga tttcaacttg
    18901 aacatcaaag ataaagaatt tatcgttttc gtaggacctt caggatgtgg taaatcaact
    18961 acactccgta tgattgctgg tcttgaagac attacagaag gtactgcatc tatcgatggc
    19021 gtggttgtca acgacgtagc tccaaaagac cgtgatatcg ccatggtatt ccaaaactac
    19081 gctctttacc cacacatgac tgtttatgac aacatggctt tcggtttgaa attgcgtaaa
    19141 tacagcaaag aagacattaa caaacgtgtt caagaagcag ctgaaatact tggattgaaa
    19201 gaattcttgg aacgtaaacc agctgacctt tcaggtggtc aacgtcaacg tgttgccatg
    19261 gggcgtgcga ttgtccgtga tgcgaaagta ttcttgatgg acgaaccttt gtcaaacttg
    19321 gatgccaaac ttcgtgtatc aatgcgtgct gaaatcgcta aaattcaccg tcgtatcgga
    19381 gctacaacta tctatgtaac tcacgaccaa acagaagcga tgacacttgc agaccgtatc
    19441 gttattatgt cagctactaa gaaccctgct ggtacaggta ctatcggacg tgtagaacaa
    19501 atcggtactc ctcaagaagt ttacaaaaat ccagttaaca aattcgttgc aggattcatc
    19561 ggaagcccag ctatgaactt catcaacgtg aaattggttg gtagcgaaat tgtttctgac
    19621 ggtttccgtt tgaaagtgcc agaaggagca ttgaaagttc ttcgtgaaaa aggctacgaa
    19681 ggaaaagaat tgatctttgg tatccgtcca gaagacgtga atgcagaacc tgctttcctt
    19741 gaaacattcc cagactgtgt tgtaaaagcg actatctctg tatcagaact gcttggttca
    19801 gaatctcacc tttactgcca agttggtaaa gacgagtttg ttgcaaaagt tgatgctcgt
    19861 gactacttgc aaacaggtgc aacagttgag cttggatttg acttgaacaa agcacacttc
    19921 ttcgatgtag aaactgaaaa aacaatctac taaaataaat aaaattcaaa gcactacaag
    19981 aaaagatatc tctttatcaa ttgtagtgga gagatatcag ttaatctagg gagagaaaca
    20041 aaatgcttct ctcctttttg ctagggaagt catattatgc atctatattg tgatgctctt
    20101 taatactctt cgaaaatctc ttcaaaccac gtcaacgtcg ccttgccgta cgtatgatta
    20161 ctgatttcgt cagttttatc tgcaacctca aagatgtact ttgagcagct tacggctagt
    20221 ttcctagttt gctctttgat ttccattgag tattatttgt gggtaccatc tacaagtgaa
    20281 gctatatgcg taaactacgt gagcaattga attcgaacta gagaggtaat aataaattta
    20341 tgctatagtt atggtgactt gtatgctttt gattctagtt tatcaaataa tagattagaa
    20401 ttgtcagata atatcatttt gtgttataat gaagaaaaaa cagaggtgtt caaatgtcag
    20461 aagcaggtca taagttttta gcaaaattgg ggaaaaaacg cttacgtcca ggtggaaagc
    20521 gtgccacaga ttggttaatt gcagaaggag gattttcaaa agaaaagaga atactagagg
    20581 ttgcgtgtaa taggggaact acagcaattg agttggcaca gcgttttggt tgcaagataa
    20641 ctgctgttga tatggatgct caagctttag aagtggctaa aaaatctgct ggaacggcag
    20701 gtgttgctca tttaatcagt tttgaaagag caaatgcaat gaaacttcct tatcaagatg
    20761 ctagttttga tattgttata aatgaagcta tgctgactat gcaagccgat caagctaaga
    20821 aaaaatgtgt aatggaatat ctaagggtat taaaacctgg aggtcttctc ttgacacatg
    20881 atgtgcttct taaggaagct aaagagtcta tcagacagga attatcacaa gcaattcatg
    20941 taaatgtagg tcctttaact caagatggtt gggaacaggt gatgatagaa tcaggttatt
    21001 gtgatgtgaa agcattgact ggtgaaatga cattaatgaa attatcgggt atgatttatg
    21061 acgaaggttt gctaggaact ttgaaaattt gtgtaaatgc ttgtaaaaag gagaatagaa
    21121 agcagttttt aactatgtat aaaatgtttg ctaagaataa acagaaattg ggctttattg
    21181 cgatggctag ttataaatcg tcaaaacgtt agataattat tgaagttaac ttttcctttt
    21241 ttctttctta aaaaatatgc tataatagag agtaaaaaac tttgaaagaa agaaaaaaga
    21301 tgaatttaaa agattacatt gcaacaattg aaaattatcc aaaggaaggc attaccttcc
    21361 gtgatattag tcctttgatg gctgatggaa atgcttatag ctacgctgtt cgtgaaatcg
    21421 ttcagtatgc tactgacaag aaagtcgaca tgatcgtggg acctgaagct cgtggattta
    21481 tcgtgggttg tccagttgcc tttgagttgg gaattggttt tgcgcctgtt cgtaagccag
    21541 gtaaattgcc acgtgaagtt atttctgctg actatgaaaa agagtacggt gtcgatacct
    21601 tgactatgca cgcggatgcc attaagccag gtcaacgtgt tcttattgta gatgaccttt
    21661 tggcgacagg tggaactgtt aaggcaacta tcgagatgat tgaaaaactt ggtggtgtta
    21721 tggcaggttg tgccttcctt gttgaattgg atgaattgaa cggccgtgaa aaaattggtg
    21781 actacgacta caaagttctt atgcattatt aatgaaaaca gtccctaggg ctgttttctc
    21841 tacactatga tataaaaata gactataact agttagagaa aaactataat tgaaaactat
    21901 atcttcttgc agtataataa aaggactaag tgtttgagat ttgtcttcaa acatatgcaa
    21961 ttattcctga aagagtacag ttaggagagg gttatgccga ttcgaattga taaaaaattg
    22021 ccagctgttg agattttacg gacagagaat atctttgtca tggatgatca acgtgctgcc
    22081 caccaagata tccgtccttt gaagatttta attttaaatc tcatgccaca gaaaatggtc
    22141 acagagaccc agttgttgcg ccacttggct aatacacccc tacaactgga tattgatttt
    22201 ctctatatgg agagccaccg ttctaaaaca actcgttcag agcacatgga gaccttctat
    22261 aaaacttttc ctgaagtcaa ggatgagtat tttgatggga tgatcatcac gggtgctcca
    22321 gttgagcatt taccatttga ggaagtggac tattgggagg aatttagaca gatgcttgag
    22381 tggtctaaga ctcatgtcta ttcgaccctt catatctgtt ggggggctca ggctgggctt
    22441 tatctgcgct atggtgtaga aaaataccag atggacagta agctatcagg tatttatcct
    22501 caggacaccc taaaagaggg tcaccttcta tttagaggct ttgatgatag ctatgtatcc
    22561 cctcattcac ggcacacgga gatttctaag gaagaggtct taaacaagac caatctcgag
    22621 attttatcag aaggacctca ggttggggtt tctattttgg ccagtcgtga tttacgagaa
    22681 atttatagtt ttggtcattt ggagtatgac cgtgatacct tggcaaaaga gtattttcga
    22741 gatcgtgatg caggtttcga cccacatatt ccagaaaatt actttaaaga tgatgatgtt
    22801 aatcaggtac cttgtctttg ttggtcttca tctgcagccc tctttttcag taattgggta
    22861 aaccatgcgg tctatcagga gacgcctttt gattggagaa agatagaaga tgatgcatct
    22921 gcatatgggt atttataaga ggaattatga catatttaga cgcttttaaa tcaggtacct
    22981 tggttttacc gagtgccctg ctcttgcatt ttaaggaact ctttccttct agcgacgatt
    23041 ttctggtttg gcaatttttc tatttgcaaa atacgacagg cttagaagaa atgtcgccaa
    23101 gccagattgc tgaaaggatt ggcaaggaaa tttcggatgt caaccagtcc atttctaatc
    23161 tgacggaaag gggactgctc cagtatcgta ctatcgaatt aaatggcgaa attgaattgc
    23221 tctttgatgc tagtttggcc ttggaacgtt tggatgacct gtttggagca gttcattcaa
    23281 gttcagacca gctaacatct caaaaccagc tcaaggattt ggtggaaacc ttccagcagg
    23341 agttgggacg attgttgacg ccttttgaga ttgaggattt gaccaagaca ctaaaggaag
    23401 atggaaccag tgctgacttg attaaggagg ctcttcgtga agctgttttg aatggaaaac
    23461 caaactggaa gtacattcag gcgattttga gaaactggcg ccatgaagga atcaagagtg
    23521 tggctcaaat tgaggccaag agagcagaaa gagaagcaag caatcctcag ttgacacagg
    23581 tatctgcaga tttcagaaat gccatggatc tctggaagga ttaatccatg caagtaggct
    23641 tgaaatccga gtaagatttg caagctgtgt ataattgtga tagaataaat agaaaataaa
    23701 ttgaaaaaag aggtatgtga aatgtcacgt aaaccattta tcgctggtaa ctggaaaatg
    23761 aacaaaaatc cagaagaagc taaagcattc gttgaagcag ttgcatcaaa acttccttca
    23821 tcagatcttg ttgaagcagg tatcgctgct ccagctcttg atttgacaac tgttcttgct
    23881 gttgcaaaag gctcaaacct taaagttgct gctcaaaact gctactttga aaatgcaggt
    23941 gctttcactg gtgaaactag cccacaagtt ttgaaagaaa tcggtactga ctacgttgtt
    24001 atcggtcact cagaacgccg tgactacttc catgaaactg acgaagatat caacaaaaaa
    24061 gcaaaagcaa tctttgcgaa cggtatgctt ccaatcatct gttgtggtga atcacttgaa
    24121 acttacgaag ctggtaaagc tgctgaattc gtaggtgctc aagtatctgc tgcattggct
    24181 ggattgactg ctgaacaagt tgctgcctca gttatcgctt atgagccaat ctgggctatc
    24241 ggtactggta aatcagcttc acaagacgat gcacaaaaaa tgtgtaaagt tgttcgtgac
    24301 gttgtagctg ctgactttgg tcaagaagtc gcagacaaag ttcgtgttca atacggtggt
    24361 tctgttaaac ctgaaaatgt tgcttcatac atggcttgcc cagacgttga cggtgccctt
    24421 gtaggtggtg cgtcacttga agctgaaagc ttcttggctt tgcttgactt tgtaaaataa
    24481 tcagtaagta gcaaaagcta ggtggaacag cattcagatg tctgttacat tttttatagg
    24541 agagcaaaga ttgaaaacaa aaattggatt agcaagtatc tgtttactag gcttggcaac
    24601 tagtcatgtc gctgcaaatg aaactgaagt agcaaaaact tcgcaggata caacgacagc
    24661 ttcaagtagt tcagagcaaa atcagtcttc taataaaacg caaacgagcg cagaagtaca
    24721 gactaatgct gctgcccact gggatgggga ttattatgta aaggatgatg gttctaaagc
    24781 tcaaagtgaa tggatttttg acaactacta taaggcttgg ttttatatta attcagatgg
    24841 tcgttactcg cagaatgaat ggcatggaaa ttactaccta aaatcaggtg gatatatggc
    24901 ccaaaacgag tggatctatg acagtaatta caagagttgg ttttatctca agtcagatgg
    24961 ggcttatgct catcaagaat ggcaattgat tggaaataag tggtactact tcaagaagtg
    25021 gggttacatg gctaaaagcc aatggcaagg aagttatttc ttgaatggtc aaggagctat
    25081 gatgcaaaat gaatggctct atgatccagc ctattctgct tatttttatc taaaatccga
    25141 tggaacttat gctaaccaag agtggcaaaa agtgggcggc aaatggtact atttcaagaa
    25201 gtggggctat atggctcgga atgagtggca aggcaactac tatttgactg gaagtggtgc
    25261 tatggcgact gacgaagtga ttatggatgg tgctcgctat atctttgcgg cctctggtga
    25321 gctcaaagaa aagaaagatt ttaatgtcgg ctgggttcac agagatggta aacgctattt
    25381 ctttaataat agagaagaac aagtgggaac cgaacatgct aagaaaatca ttgatattag
    25441 tgagcacaat ggtcgtatca atgattggaa aaaggttatt gatgagaacg aggtggatgg
    25501 tgtcattgtt cgtctaggtt atagcggtaa agaagacaag gaattggcgc ataacattaa
    25561 ggagttaaac cgtctgggaa ttccttatgg tgtctatctc tatacctatg ctgaaaatga
    25621 gaccgatgct gagaatgacg ctaaacagac cattgaactt ataaagaaat acaatatgaa
    25681 cctgtcttac cctatctatt atgatgttga gaactgggaa tatgtaaata agagcaagag
    25741 agctccaagt gatacagaca cttgggttaa aatcatcaac aagtacatgg acacgatgaa
    25801 gcaggcgggt tatcaaaatg tgtatgtcta tagctatcgt agtttattac agacgcgttt
    25861 aaaacaccca gatattttaa aacatgtaaa ctgggtagcg gcttatacga atgctttaga
    25921 atgggaaaac ccttattatt caggagaaaa aggttggcaa tatacctctt ctgaatacat
    25981 gaaaggaatc caagggcgcg tagatgtcag cgtttggtat taagcgatga tttgaaagag
    26041 ggatgtgata gtagcaccct ctttttcttt gttttatgat agttcatcct cgagtaaatt
    26101 caagatcttg ctcggaaatg aagcttatat agtagattga atatagacaa ataccttgtg
    26161 attggtaaaa cattttagaa attcatttac ctttcctaat cgacttggtt tcatcttatt
    26221 tcaatctatt atagtattgg ggaatttctt caaaccacat cagcttggtc agttctacct
    26281 gcgacctcaa aacttgtgct ttggtcaagc tgggtttagt ttcctagttt gctgatggat
    26341 ttccattgac tataagcatc caaccctctt tttgtcttct aaagaattct taaattatca
    26401 gtctattgca acttttctca tataagttct ttgtcttgct attggttttc cttagtagta
    26461 tactaaggta gtaatcatta agaagtggtt acaaaaaata atgaatgagg taaaataaaa
    26521 tggtagaatt gaaaaaagaa gcagcaaaag atgtcgcaac attgacaaaa gcagcgccag
    26581 tagcattggc aaaaacaaag gaagtcttga accaagctgt tgctgatttg tatgtagctc
    26641 acgttgcttt gcaccaagtg cactggtata tgcatggtcg tggtttcctt gtatggcatc
    26701 caaaaatgga tgagtacatg gaagctcttg acggtcaatt ggatgaaatc agcgaacgct
    26761 tgattacact cggtggaagc ccattctcta ctttgacaga gttccttcaa aacagtgaaa
    26821 tcgaagaaga agctggtgaa taccgtaatg ttgaagaaag cttggaacgt gttctagcta
    26881 tttatcgcta cctcatcact cttttccaaa aagcgttgga tgtgactgac gaagaaggcg
    26941 atgatgttac aaacgatatt tttgttggtg ctaaggctga acttgagaaa acagtttgga
    27001 tgcttgccgc agaacttgga caagcacctg gtttgtaaga aacaagacaa catagataaa
    27061 tctgtaggaa atttcttaca gattttttct attttgtaag ctattccaaa ccagtctttt
    27121 tttgagtttt ttgataaaat agtactatca gtgaaaagga tggaagcatg actaagaaaa
    27181 tcgtagctat ttgggcccag gatgaagagg gtgtgattgg taaggacaat cgtctgcctt
    27241 ggcatttgcc agcagaattg cagcacttta aagaaacaac tctgaatcat gctatcttga
    27301 tggggcgggt aacctttgat ggaatggggc gtcgcttgct tccaaaacgg gagaccctga
    27361 ttttgacgcg taatccggaa gaaaagatag atggggttgc tacttttcag gacgtccagt
    27421 ctgttcttga ctggtatcag gctcaagaaa agaatctcta cattatcggt gggaagcaaa
    27481 tttttcaggc ttttgaagct taccttgatg aagtgattgt cactcacatt catgctcggg
    27541 tggaaggaga tacctatttc cctgaagagc ttgacttgtc tctttttgag acagtttcaa
    27601 gcaaatttta cgccaaagat gagaagaatc cttatgattt taccatccaa taccgcaaga
    27661 gaaaggaagt ttaatggaac ggagtttatt tggtctattc acggcttttc tgtgtttcat
    27721 ctgctttctg gcaggagcac aagcttttcg taaaaagcgt tacgggcttt ctatcctcct
    27781 ttggttaaat gcctttacca atctggtcaa tagtattcat gctttttaca tgaccttatt
    27841 ttagatagaa tgacagtata gaatggaagg aaatcatgtc tacaaataga aaaaatgata
    27901 tgatggttta ttgctcattt tgtggcaaaa accaagaaga agtacaaaaa ataattgctg
    27961 gcaacaatgc ttttatttgt aatgaatgcg tggagttagc tcaggaaatc attcgagaag
    28021 aattggttga ggaagtcttg gcagacttgt ctgaggtgcc aaaaccaatt gaactcctcc
    28081 atatcttgaa ccactatgta attggtcaag atcgtgccaa gcgtgccttg gcagtggcgg
    28141 tttataacca ctacaaacgc atcaatttcc acgatacacg cgaagagtca gaagatgtgg
    28201 atttgcagaa gtcaaacatt ttgatgattg gcccaactgg ttcagggaaa actttccttg
    28261 cccagacctt ggctaagagc ttgaatgtac cttttgctat tgcggatgcg acagctctga
    28321 cggaggctgg ttatgtgggt gaggatgtgg aaaatatact cctcaaactc ttgcaggttg
    28381 ctgactttaa catcgaacgt gcagagcgtg gcattatcta tgtggatgaa attgacaaga
    28441 ttgccaagaa gagtgagaat gtgtctatca cacgtgatgt ttctggtgaa ggggtgcaac
    28501 aagcccttct caagattatc gagggaactg ttgctagcgt accgcctcaa ggtggacgca
    28561 aacatccaca acaagagatg attcaagtgg atacaaaaaa tatcctcttc atcgtgggtg
    28621 gtgcttttga tggtattgaa gaaattgtca aacaacgtct gggtgaaaaa gtcatcggat
    28681 ttggtcaaaa caataaggcg attgacgaaa acagctcata catgcaagaa atcatcgctg
    28741 aagacattca aaaatttggt attatccctg agttgattgg acgcttgcct gtttttgcgg
    28801 ctcttgagca attgaccgtt gatgacttgg ttcgcatctt gaaagagcca agaaatgcct
    28861 tggtgaaaca ataccaaact ttgctttctt atgatgatgt tgagttggaa tttgacgacg
    28921 aagcccttca agagattgct aataaagcaa tcgaacggaa gacaggggcg cgtggacttc
    28981 gctccattat cgaagaaacc atgctagatg ttatgtttga ggtgccgagt caggaaaatg
    29041 tgaaattggt tcgcatcact aaagaaactg tcgatggaac ggataaaccg atcctagaaa
    29101 cagcctagag gtgactatgg aacttaatac acacaatgct gaaatcttgc tcagtgcagc
    29161 taataagtcc cactatccgc aggatgaact gccagagatt gccctagcag ggcgttcaaa
    29221 tgttggtaaa tccagcttta tcaacactat gttgaaccgt aagaatctcg cccgtacatc
    29281 aggaaaacct ggtaaaaccc agctcctgaa cttttttaac attgatgaca agatgcgctt
    29341 tgtggatgtg cctggttatg gctatgctcg tgtttctaaa aaggaacgtg aaaagtgggg
    29401 gtgcatgatt gaggagtact taacgactcg ggaaaatctc cgtgcggttg tcagtctagt
    29461 tgaccttcgt catgacccgt cagcagatga tgtgcagatg tacgaatttc tcaagtatta
    29521 tgagattcca gtcatcattg tggcgaccaa ggcggacaag attcctcgtg gtaaatggaa
    29581 caagcatgaa tcagcaatca aaaagaaatt aaactttgac ccgagtgacg atttcatcct
    29641 cttttcatct gtcagcaagg cagggatgga tgaggcttgg gatgcaatct tagaaaaatt
    29701 gtgaggaaaa gaaaatggca aaaacaattc atacagataa ggccccaaag gctatcggac
    29761 cctatgttca aggaaaaatc gttggcaacc ttttgtttgc tagcggtcaa gttcccctat
    29821 cccctgaaac tggggaaatt gtaggagaga atatccaaga acagacagag caagtcttga
    29881 aaaacatcgg tgctattttg gcagaagcag gaacagactt tgaccatgtt gtcaaaacaa
    29941 cttgtttctt gagcgatatg aacgactttg ttccttttaa tgaggtttac caaacggcct
    30001 tcaaagagga attcccagct cgttcagctg tggaggtagc tcgtcttcct cgtgatgtaa
    30061 aagtcgaaat tgaagtcatc gcagagattg gataagctag ttgaagtttg gtgttgccaa
    30121 acttcttttg atataaggag aaaaagatga caaagaaaca acttcacttg gtgattgtga
    30181 cagggatgag tggcgcaggg aaaactgtag ccattcagtc cttcgaggat ctaggttatt
    30241 tcaccattga taatatgccg ccagctctct tgcctaagtt tttgcagctg gttgaaatta
    30301 aggaagacaa tcctaagttg gccttggtag tggatatgcg tagccgttct ttcttttcag
    30361 agattcaagc tgttttggat gagttggaaa atcaagatgg tttggatttc aaaatcctct
    30421 ttttggatgc ggctgataag gaattggtcg ctcgttacaa ggaaaccaga cggagtcacc
    30481 cactagcagc agacggtcgt attttagatg gaatcaagtt ggaacgtgaa ctcttggcac
    30541 ctttgaaaaa tatgagccaa aatgtggtgg atacgactga actcactcca cgtgagctgc
    30601 gcaaaaccct tgcagagcag ttttcagacc aagaacaagc ccagtctttc cgtatcgaag
    30661 tcatgtcttt cggatttaag tatggaatcc cgattgatgc ggacttggtc tttgatgtcc
    30721 gtttcttgcc aaatccctat tatttaccag aactgagaaa ccaaacgggt gtggatgaac
    30781 ctgtttatga ttatgtcatg aaccatcctg agtcagaaga cttttatcaa catttattgg
    30841 ccttgattga gccgattctg ccaagttacc aaaaggaagg taagtccgtt ttgaccattg
    30901 ccatgggatg tacgggtgga caacaccgta gtgtggcatt tgctaaacgc ttggtgcagg
    30961 acttatccaa gaattggtct gttaatgaag ggcatcgcga caaagaccgc agaaaggaaa
    31021 cggtaaaccg ttcatgagaa aaccaaagat aacggtgatt ggtggaggga ctggaattcc
    31081 cgtcattcta aaaagtctgc gggaaaaaga tgtggaaatc gcagctatcg tgacggtggc
    31141 agatgatggt ggttcttcag gtgaactccg aaaaaatatg caacagttga caccgccagg
    31201 tgattttcgt aatgtccttg tggccatgtc ggatatgcct aagttttatg agaaggtctt
    31261 tcagtatcgg ttctctgagg atgccggagc ctttgctggc catccattgg gaaatctcat
    31321 cattgctggc ttgtcagaaa tgcagggttc aacctataat gccatgcagt tattgagcaa
    31381 atttttccat acaacaggga aaatttatcc ttccagtgac catcctttga cccttcatgc
    31441 agtctttcag gatgggacag aagtggctgg agagagtcat attgtagacc atcgaggcat
    31501 aattgacaat gtctatgtga ccaatgccct aaacgatgat acgcctctgg ccagccgtcg
    31561 agtagtgcag accatccttg aaagtgacat gattgtccta gggccaggtt ccctctttac
    31621 ctctattttg cccaatatcg tgattaagga aattgggcgg gctcttttgg aaaccaaggc
    31681 agaaattgcc tatgtctgca atatcatgac ccaacgtggg gagacggaac actttacaga
    31741 tagcgaccac gtggaagtct tgcatcgtca ccttggtcgc ccttttatcg acactgtctt
    31801 ggtgaatatt gaaaaagtgc ctcaggaata catgaattcc aaccgttttg atgaatactt
    31861 ggtgcaagtg gaacacgatt ttgtaggtct ttgtaagcaa gtttcgcgcg tgatttcatc
    31921 taacttcctt cgtctggaaa atggcggtgc cttccacgat ggagatttga ttgtggacga
    31981 gttgatgcgc attatacagg tgaaaaaatg agtttcacag tagcagtaaa agaagaaatc
    32041 ctgggccaac accatctgag ctggcatgaa ttatctgcca ttatcaaaat gtctggtagc
    32101 atcggtctct cgacttcggg cttgactttg tcagttgtga cagaaaatgc caaactggcc
    32161 cgtcacctct atgagtcctt tctccatttc tatgaaatca aatctgaaat tcgtcaccac
    32221 caacggagca atcttcgcaa gaatcgcgtc tataccgttt ttacagatga aaaggtgcag
    32281 gatttgttaa gtgatttgca cttggcagac tctttctttg gcctggaaac aggtattgat
    32341 gaggcgattt tatcggatga ggaagcaggt cgtgcctatc tctgtggcgc tttcttggca
    32401 aatgggagca ttcgtgaccc tgagtcaggc aagtaccagt tggaaatcag ttctgtttat
    32461 ctggaccacg cgcaaggaat tgcctccctt ctccaacagt ttttactgga tgccaaggtg
    32521 cttgagcgca agaagggggc tgtgacctat ctccagcgag cagaagacat tatggacttc
    32581 ttgatagtaa tcggagccat gcaggcacgt gatgattttg agcgggttaa gattttgcga
    32641 gaaacccgta acgacctcaa tcgggctaat aatgccgaga cagctaatat cgctcggaca
    32701 gtttctgcca gcatgaagac cattaacaat atcagtaaaa tcaaagatat catgggctta
    32761 gaaaatttgc cagtagattt gcaggaagtg gcgcaactga ggattcagca cccagactac
    32821 tctatccagc agttagcaga tagtctcagc acccctctga ccaaaagtgg tgtcaatcac
    32881 agactcagaa aaatcaataa gatagcggat gaattataaa accacgaatc ctatgtgact
    32941 cgtggttctt ttttataaac tggtagagtg ttttggttgt actttttgtt cagggtaaat
    33001 gtagttgata gcgttgttga cagcagttgg agcttctccg aggcctgtcg caatcagatc
    33061 aatttttccg tcatagtagc agcagtcacc gatagcatag atacctgctt ggctggattc
    33121 ctgtttgctg tttacgataa tcttgtgacg gttgaggtcg agcccccagt ttttaaggtt
    33181 accgacagaa gatttgaaac catagttgac aaagaggtgg tctaggtcaa tggtttcagt
    33241 ttcatcagat ttgacttttg tgatttcaag tttatcaagt gtttttccat ttccaaggag
    33301 ttggctaggg gcgaatggtg tcttgatggt tacagatgat tcttgcaagg cttgaacact
    33361 gtgttccaag gcacggaaat tatctctgcg gtgaacaagg gtagttggtg cgattttttc
    33421 aaaagccaaa gtccaatcca cagccgagtc tcccccgcca agaatcgtca ctttcttacc
    33481 agcgtattgc tgaatgttag aaacgtggta gtggatattt tcatagccct caaccccttc
    33541 aagttccagc ggacgtggtt tgaaggcacc gccacccata gcaatgataa ctgttttagt
    33601 caggtgactt cctttagaag ttgtgatggc aaattcttct tcttgtttgt caatttcaag
    33661 aaccgtttca ttgagatgaa taggggtatc aaatccattt agctgttcaa tcaagcggtt
    33721 agtcaactct tctccagtca ggtttgggaa gcctggtacg tctaggattt ccttttcagg
    33781 gtagagaata gcaggttgtc cacctagctg gggaagagag tcgatgattt gaaccttggc
    33841 ttggcgtagg tgggcataaa aggctgcaaa aagcccgaca ggaccaccac ccacaatggt
    33901 aatatcatag agttgagaca tagtttctcc tttgtttttt ctagtcagtt tattttatca
    33961 tatttttgtt caatgtaaaa tggactagga tagggaaaaa aatgccagaa aatggtataa
    34021 taggacgaaa gtgttttgat agggaggata tcagggatga attttcaaca attatccaat
    34081 ctgcaatatt ggaccagttt gtttgcaagt ccatggacga tagctatcaa tctgattgat
    34141 attttgattg ttgcttatat tttataccat tttacaaaag ctattgcagg aaccaagatt
    34201 atgattttgg tacgtggagt tttggtgttt attttagctc aaatccttgc aaatatgatt
    34261 ggtttgacta cgatttcttg gttaatcaat caaattatta cttatggggt tattgcggcg
    34321 gttgttatct tctctccaga gattcggact ggtttggaac gtttgggaag agcgacagat
    34381 ttcttttcca atgcccctat tagtgctgag gaacagatga ttcgtgcctt tgttaagtct
    34441 gttgaataca tgagtcctcg taaaatcggg gccttggttg ctattcagcg tgtacgtacc
    34501 ttgcaggagt atatgtcgac aggaattccc ttggatgcta agatttctgc agaacttctc
    34561 attaacattt ttattcccaa cactccccta catgacggtg cggtgattat caaagaagaa
    34621 cgtatcgctg tgacgtctgc ctatctgccc ttgacaaaaa acacagggat ttccaaggaa
    34681 tttgggacca gacaccgggc ggctatcggt ttatcagaag tctcagatgc cttgactttt
    34741 gtcgtatcag aggaaacggg aggaatttcg ataacctata atggaaggtt taagcacaac
    34801 ctaacacttg atgaatttga aacagaatta cgtgaaatct tacttccaaa agaggaagtg
    34861 ggtcttagtt ttaaagaacg attgctagga ggatggaaac atgaaaaaaa atagtttata
    34921 tatcatatcc tcactctttt ttgcttgtgt cttatttgtc tatgctacgg cgacgaattt
    34981 tcaaaacagt accagtgcta ggcaggtaaa aacggaaacc tatactaata cagtaacaaa
    35041 tgtccctatt gacatacgct ataatagtga taagtatttt attagcggtt ttgcttcaga
    35101 agtatcagtg gtcttgactg gtgcaaatcg tctatcgcta gctagtgaaa tgcaagaaag
    35161 tacacgtaaa ttcaaggtta ctgctgacct aacagatgcc ggtgttggaa cgattgaagt
    35221 tcctttgagc attgaagatt tacccaatgg gctgaccgct gtggcgactc cgcaaaaaat
    35281 tacagtcaag attggtaaga aggctcagaa ggataaggta aagattgtac cagagattga
    35341 ccctagtcaa attgatagtc gggtacaaat tgaaaatgtc atggtgtcag ataaagaagt
    35401 gtctattacg agtgaccaag agacattgga tagaattgat aagattatcg ctgtcttgcc
    35461 aactagcgaa cgtataacag gtaattacag tggttcagta cctttgcagg caatcgaccg
    35521 caatggtgtt gtcttaccgg cagttatcac tccgtttgat acaataatga aggtgactac
    35581 aaaaccagta gcaccaagtt caagcacatc aaattcaagt acaagcagtt catcggagac
    35641 atcttcgtca acgaaagcaa ctagttcaaa aacgaattaa aaatagaaaa ggattttata
    35701 acaatgggta aatattttgg gactgatgga gtccgtggag aagctaacct agaactaaca
    35761 ccagaattag cctttaaact aggacgtttt ggaggctatg ttcttagtca acatgaaacg
    35821 gaagcgccga aagtctttgt aggacgtgac acacgtattt caggggaaat gctggaatcg
    35881 gccttggtgg caggtctcct ttcagtaggg attcacgtat acaaacttgg tgtccttgca
    35941 acaccagcag tagcttactt ggttgaaact gaaggagcaa gtgccggtgt catgatttct
    36001 gctagccaca acccagccct tgataacgga atcaagttct ttggcggtga tggcttcaaa
    36061 ctagatgatg aaaaagaagc agaaattgaa gccttgctag atgctgagga agacactctt
    36121 cctcgtccaa gtgcagaagg tttaggaatt ttggtagatt atccagaagg cttgcgtaag
    36181 tatgaaggat accttgtttc aactggaact cctcttgatg gaatgaaggt tgccttggat
    36241 acagctaatg gagcagcttc taccagtgcc cgtcaaatct ttgcagacct tggtgcccaa
    36301 ttgacggtta tcggggaaac accagacggt cttaacatca accttaatgt tggttcaaca
    36361 catccagaag cccttcaaga agtggtcaaa gaaagtgggt cagctattgg tttggccttt
    36421 gatggagaca gtgaccgctt gattgctgtt gatgagaatg gtgacatcgt cgatggtgac
    36481 aagattatgt acatcatcgg aaaatacctt tctgaaaaag gacaattggc tcaaaataca
    36541 attgtgacaa ctgttatgtc taaccttggt ttccacaagg ccttgaatcg cgaaggtatt
    36601 aacaaggcag ttactgcagt tggtgaccgc tacgttgttg aagaaatgag aaaatcaggt
    36661 tacaaccttg gtggtgaaca gtctggtcac gttatcttga tggattacaa taccacaggt
    36721 gatggtcaat tatcagcagt tcaattgact aaaatcatga aggaaactgg taagagctta
    36781 tcagagttgg cggcagaagt aacgatttat ccacaaaaat tagttaatat ccgagtggaa
    36841 aacgtcatga aggaaaaggc catggaagtg ccagctatca aggccatcat cgagaagatg
    36901 gaagaagaaa tggcggggaa cggccgtatc cttgttcgtc caagtggaac agaacccctc
    36961 ttgcgtgtta tggcagaagc gcctacaaca gaagaagtag actactatgt tgataccatc
    37021 acagatgtag ttcgtgctga aattgggatt gactaaatcc tagaaaacta gatgaaaata
    37081 aaaaattatg gtacaatagc ttataatatt gtagccgatg gagaaagaca tgaatcttaa
    37141 acgtgaacaa gaatttgtta gtcagtatca ttttgatgcg cgtaattttg aatgggaaaa
    37201 tgaaaatgga gctcctgaaa ccaaggtgga tgtgaatttc caattgctcc aacatgacca
    37261 agaaaatcaa gtgacttcct tgattgtcat cttgagcttt atgattgtat ttgataagtt
    37321 tgtcattagt ggaacaatct cacaagtaaa ccatatcgat ggccgtattg tcaatgaacc
    37381 aagtgaattg aaccaagaag aggtggaaac cttggctcgt ccatgtttga acatgctcaa
    37441 tcgtttgact tacgaagtga ctgaaattgc attagactta ccaggaatta atttggagtt
    37501 ctaatatgaa attagctgtt ttcacagatt cctctgctta tctcagtgca gagaccttgc
    37561 aaagagaaga cttgtttgtc ttggatattc ctgtcaatat tgatggtgag gaatatgtcg
    37621 aaggcatcaa tctgtctgct gaggaatttt accaaaaaat ggctcaggct tctgaattgc
    37681 ctaagaccag tcaaccaagt attgccaagt tagatgaaat cttaacttcg ctcaaagaac
    37741 aaggctatac acatgccttg gggcttttcc tatcttctgg aatttcaggt ttttaccaaa
    37801 atatccagta tatggtcgat gactatgagg gcttaaccat tgctttccca gacactttga
    37861 ttacaagtgc tcccctaggc atcatggttg aaagcgtctt taattggcgt gatcagggcg
    37921 atgattttgc cagcattcag gataagctag ccattcaaat cagccgtacg tcagccttta
    37981 tcatggtaga tgacttggat catttggtaa aaggtggacg cctttcaaat ggggctgcca
    38041 ttttgggcaa tttgcttagc attaagccaa tcctttattt taacgatcaa ggtgtgattg
    38101 aagtttacga aaaagttcgt actgaaaaga aggccaccaa acgcttaatt gaaattatca
    38161 aggaaacaac ggcttcaggt caataccggg tcattgtcat tcatgggaat gctcctgaaa
    38221 aggctgaaga attgcgtcag catttgcttg attttggctt gggttcggat gtttcacttg
    38281 ctacatttgg tagtgtcatt ggaacgcacc taggagcagg aagtattgct ctgggttata
    38341 ttccagtgat ttagtaggca cttctaggct agttaatctt agcaattgaa cacggactac
    38401 ctgcctcttg aaaaaagatg tctgtcttgc ctttcgtgga aagtcagtgc cattccctat
    38461 ttttcatggg tggctaagat cctttgtatc ttgataattg aacacgcctg gaaccctgtg
    38521 tgaaaaagat agttcttcca aggagtagac acttcttgat cagaattcct attttcactt
    38581 tgtgttctta caggctttgt atcttagaca ggagtagaga tgagtattcg agtaattatt
    38641 gccggtttta agggaaagat gggtcaagct gcttgccaga tggtcttgac tgattcagat
    38701 ttggacttgg tagcagtttt ggatcctttt gagtctgagt cagaatggca gggtattcct
    38761 gttttcaagg ataaggctga tttagctggt tttgaagcgg atgtctgggt agactttact
    38821 actccagctg ttgcctacga aaatacacgt tttgctcttg aaaatggctt tgctccagta
    38881 gttggaacga ctggtttcac gagtgaagaa attgcagagc taaaagaatt ttctcgtgcc
    38941 caagacttgg gtggcctgat tgcccctaac tttgccttgg gtgctgtctt actcatgcaa
    39001 tttgcgacgc aggctgccaa atatttccca aatgtggaga ttattgagct ccatcatgac
    39061 aagaaaaagg atgctccgag tggaacagcc attaaaacag ctgagttgat ggcagaggtt
    39121 cgagagtcaa ttcagcaagg tgcagcagat gaggaagagc tgattgctgg tgctcgtggt
    39181 gctgactttg atggtatgcg catccactca gttcgtttgc caggtttggt agcccatcag
    39241 gaagtcatct ttggcaatca aggagaagga ttgaccctcc gtcatgactc ctatgatcgc
    39301 atctccttca tgacaggagt caatttggga attaaagaag ttgtcaagcg tcatgagctt
    39361 gtctatggat tagaacactt attatgagat taacgcaaat gccttctgaa tttcagaagg
    39421 ctttaccagt attagaaaaa attaaagaag caggctttga ggcttatttt gttgggggct
    39481 ctgttcgaga tgcccttctc catagcccta tccacgatgt ggatattgcg acgtcttctt
    39541 atccagaaga gaccaagcag atttttccgc gaacagccga tatcggaatc gagcatggaa
    39601 ccgtcttggt cttagatggg gatgaggagt atgaggtaac aacctttcgg acagaggatg
    39661 tctatgtgga ctatcgcaga cccagtgcgg tttcctttgt gcgctcgcta gaagaagacc
    39721 tcaaacgccg tgatttcaca gtcaacgcct ttgccttgga tgagacagga gaaatcgttg
    39781 acttgttcca tggtttagaa gatttggaaa agcaagtctt gcgagcagtt ggagtggcta
    39841 gtgagcgttt caacgaagat gctttacgga ttatgcgtgg tttccgtttt caggctagtc
    39901 ttggttttgc acttgagcca gaaacattta aagctatgaa gaccttgacg ccgcttttgg
    39961 agaaaatttc tgtagagcgt accttcgtcg agtttgataa actcttgctg gctccatttt
    40021 ggagaagggg cttggcttcc atgattgaga gtcaagctta tgactatctc cctgatatgg
    40081 catctagcca gaacaagctc aacagactgt ttgatttgga gactgatttt acttttgaat
    40141 cctctgaaca agcctgggcg gctttactat gggctttgga gattgaaaat gcgcagtcat
    40201 ttttgaaatc ttggaagacc tcacgccagt ttgccaagca agttcaggat ttgctgatta
    40261 ttttggctct gcgtgaaaat ggagaattga gcaagcgaga ttgttatcgc tttgacatag
    40321 atttgctttt acaggctgaa aatcttcgtc aagctcgagg aaaagaagtc aacccacaag
    40381 ccatcacaga aaaataccaa agcttgacta tccatgacaa gaaagatatt cagattaatg
    40441 gcgatatttt gatcaaggaa tatggctatc agccaggccc agacttggga gagattttaa
    40501 cagagattga gtttgccatt gtcgatggag aattggagaa taatcgtgaa gccatccatg
    40561 cttacctgag ggagaaaaaa tgagtgattt tatcgttgaa aaactaagta aatccgttgg
    40621 tgacaagacc gtttttaggg atatttcctt tattatccat gacttagaca gaattggttt
    40681 aatcggtgtc aatgggactg gcaagaccac ccttttggac gtcctttctg gtgtttctgg
    40741 ctttgatggg gatgtcagtc ctttttcagc taaaaatgat taccagattg gttacttgac
    40801 tcaggatcct gattttgatg atagaaagac agttttggat acggttctat ctagtgaact
    40861 caaggaaatc cagctcattc gtgagtatga attgattatg ctcgactata gtgaggacaa
    40921 gcaggcgcgt ttggaacgtg tcatggcaga gatggactct ctccaagctt gggaaatcga
    40981 gagtcaggtc aagaccgttc ttagcaaatt gggcattcaa gacttatcta ctcctgttgg
    41041 ggaattgtca ggtggtctga gaagacgggt acagttggca caagtcttac ttggtaacca
    41101 cgacctcttg cttttggatg agccgaccaa ccatctggat attgcgatta ttgagtggct
    41161 gaccctcttt ttgaaaaatt ctaagaagac cgtccttttt atcactcacg atcgttattt
    41221 cttagacgct ttgtcaacac ggattttcga gttggatcgt gcaggcttga ccgagtaccg
    41281 gggaaattac caggactatg ttcgcctaaa ggcggaacag gatgagcgcg acgcggctct
    41341 tcttcacaaa aaagaacaac tctacaaaca agaattggcc tggatgcgca gacaaccgca
    41401 ggcgcgtgcg accaagcagc aggcccgtat caatcgattc catgatctga aaaaggaagt
    41461 ttcaggcagt agtgctgaga cagacttgac catgaacttt gaaaccagtc ggattgggaa
    41521 gaaagtcatc gagtttcagg ctgtttcctt tgcctatgaa aacaagccta ttttgcaaga
    41581 ttttaatctc ttggtgcagg ccaaagaccg tatcggaatc gttggggaca atggtgttgg
    41641 aaaatcaacc ctgctcaact tgattgcagg aagtcttgag ccaactaaag gacaagttgt
    41701 gattggggaa actgttcgca tcgcctattt ctctcagcaa attgagggtt tggatgaaag
    41761 caagagagtt atcaattacc tgcaggaagt ggcagaagag gtcaagacca gtggcggttc
    41821 tacgacttcg attgcagagt tgctggagca gttccttttc ccacgttcga cgcatggaac
    41881 cttgattgag aaattgtcag ggggtgagaa aaaacgtctt tatctcctca aactgctttt
    41941 ggaaaaacca aatgttcttc ttttagacga gccaaccaat gacctagata ttgcaacttt
    42001 gacagtctta gagaatttct tgcaaggttt tgcaggtccc gttttaacag tcagtcacga
    42061 ccgctatttc ttggataagg tagcgaccaa gattctcgct tttgaggatg gcaagattcg
    42121 tcctttcttt ggtcattaca ccgactatct tgatgaaaaa gcttttgaaa cagatatggc
    42181 caatcaagtg caaaaggccg aaaaggaaaa agtggtcaag gttcgagaag acaagaaacg
    42241 catgacctac caagaaaagc aggagtgggc aagtattgaa ggtgatattg aagccttgga
    42301 aaaacgtatc gctgctattg aagaggaaat gcaggctaac ggctctgact ttggtaagct
    42361 ggctactctc caaaaagaat tggatgagaa aaatgaagca ctccttgaaa aatacgaacg
    42421 ctatgagtat ctcagtgaat ttgatagttg atactattcg aaaatcaaag tgcaaactat
    42481 gtcggcttca cattgccgac tcaagtacag cctgtgaccg gcttcctaat ttgcactttg
    42541 atttttattg agtataaacg aatagaaaaa tcccgtctca tgaaaggatt tctctattct
    42601 tttttggttt cttgatgaaa gatattttgc caggtttcgt ggcgacgcca gatactggta
    42661 tggatgatac catctaactc atagcagata agtttggttt tttgactgat ggaagtaatt
    42721 tgaatgtcct tgatagcaga atttaactct tttttggtct tataagcctc tttatccatc
    42781 tgctctccat cttggcgaat atagacaaaa tcatcagtca agagttcttc tagttggttt
    42841 ccttggtcga tcaattgttc acgcataagc aattttttat agacattgtc taaaatttca
    42901 tcctcaggga taggtgctgg ttcgatatgg acatcggtat caaagacgcc aaaacgattc
    42961 tccagcatag actcgacctg atccgcgatt tcatggcttt caaaaacaga caagtcagga
    43021 ttcatctcta gtgtaatatc caggtagatg ttgctaccgt aggtgcgacc tctttgcgat
    43081 ttgaccttgc tgattttggg aatttccatg atagcctttt ggtagtcctc gagcaggcgg
    43141 tcgtcaaagc catctgaaag actaaaggaa gactcgatga agatatcata ggcagtcttc
    43201 aagataaaga aagtgatgat gatagcaacc agtttatcca caatcggata attgaaacta
    43261 ctagctagga tggcaatggc ggttccaagt gaggtaacag cgtcagaaag attgtcctta
    43321 gcagctgcct tcagcgcctt ggagttggat ttcttactga ggcgagtatt gtagagatag
    43381 accacaaaca taatcgctgc agaaatgatt cctagagttg caccaagagg atcaatgacc
    43441 gtttcttccc gactgagaat cttttgaatg gtatctctta gaacatcgaa accgacatag
    43501 aacatgatga tagaagtgat caagcttgcc aaatcttcaa tcttccaatg accaaaacgg
    43561 tggtcacggt ctgcaggctg gcgcgccatc cgaatcccga ttaagagggc cacatttcca
    43621 atgatgtccg atacgttatt aaaaccatcg gccaccaaac tggatgaatg aaggagatga
    43681 ccagctgcta atttggctgc agacaagatc aaataggtcg aaatactgat aatggctcca
    43741 cgctcagcta acttgagatt tgagatagat tgcttcattc aacttccttt ctagtcacat
    43801 agcattctac cattatactg gttttcaagg aaaaattcaa ataaggcagt ttttactctt
    43861 tgaaaactcc ccaaaatttg gtataatggt actactcaag ggagtagctg gcagaaacct
    43921 gtgatagtgt cgtcattccg aattttatgc tgaaaagtat gctttccggc cctatcttaa
    43981 acagcgagac ttgttatgat taacaggtct ctttttgttt gtcgtgaaaa gatatgacag
    44041 aaagagagtc tgttttgcac acaatgtcaa ggaggagaca catgtcaaaa gaacaaaaac
    44101 gccaagcgtt ttatactcag agccctgaag aggtcttgca ggctgtggat gcgaccgagc
    44161 aaggtttgtc atcaagtgag gcggaaaagc gccttgccga atttgggcac aatgaactcg
    44221 aagaaggcga gaaacgatca atcttggtca aattcatcga gcaatttaag gatttgatga
    44281 ttatcatcct agttgcggca gcaatcttgt cagtcgtgac ttctggtggg gaagatatcg
    44341 cagatgccat tatcatccta gctgtggtta tcatcaacgc tgcctttggt gtttaccaag
    44401 aaggaaaagc tgaagaagct attgaagccc tcaaatccat gtctagtcca gttgcccgcg
    44461 ttcttcgtga tggacacatg gcagagattg actctaaaga attggtacca ggcgatatcg
    44521 ttgcccttga agcaggtgac gtggtaccag cggacctacg tttgatagaa gccaactctc
    44581 ttaaaattga agaagcagcc ttgacaggtg aatctgtacc agtcgaaaaa gacttgtcag
    44641 tcgagcttgc gacagatgct ggtattggtg accgtgtcaa catggccttc caaaactcaa
    44701 acgtaaccta tggtcgtggg atgggtgttg ttgtcaatac aggtatgtac actgaagttg
    44761 gtcatattgc tggtatgctt caagatgcgg atgagactga tacaccactc aaacaaaatt
    44821 tgaacaacct ttctaaggtc ttgacctatg ctatcttggt cattgccctt gttacttttg
    44881 tagtgggtgt cttcattcaa gggaaaaatc cacttggtga gttgttgact tctgttgccc
    44941 ttgccgttgc agccattcca gaaggacttc ctgctatcgt taccatcgtt ctttctcttg
    45001 gtactcaagt tttggccaaa cgacattcca tcgttcgtaa gttgccagca gttgaaacac
    45061 ttggttcaac tgaaatcatc gcttctgata agactggtac gctgactatg aacaagatga
    45121 cagtcgaaaa agtcttctac gatgcggttc tacatgactc agctgatgat attgaactag
    45181 gtcttgaaat gccactactt cgttcagttg tcttggccaa tgatacgaaa atcgatgtgg
    45241 aaggtaactt gattggtgac ccaaccgaaa cagcctttat ccaatatgcc ttggacaagg
    45301 gctatgatgt caaaggtttc ttagagaaat atcctcgtgt agctgaattg ccatttgact
    45361 ctgaccgtaa gctcatgtca acagttcacc cattgccaga tggtcgtttc cttgtagcag
    45421 tcaagggtgc gccagaccaa ctcttaaaac gttgtcttct tcgtgataag gctggggata
    45481 ttactccgat tgatgagaag gttacaaatc tcattcatac aaacaattct gaaatggctc
    45541 atcaagcctt gcgtgtcctt gcaggtgctt ataagattat cgatagtatt ccagaaaatc
    45601 tcacttctga agagcttgaa aatgatttaa tttttactgg tttgattggg atgattgacc
    45661 ctgaacgtcc tgaagccgct gaggctgttc gtgtggctaa ggaagcggga atccgtccaa
    45721 ttatgattac aggtgaccac caagacacag cggaagccat tgccaaacgt ttgggaatca
    45781 ttgacgcaaa cgatacagaa ggtcacgttt taactggtgc tgaactcaat gaactgtcag
    45841 atgaagaatt tgaaaaagtc gttggtcaat actctgttta tgcccgtgtg tctccagaac
    45901 acaaggttcg tatcgtcaag gcttggcaaa aacaaggtaa agtcgttgcc atgacaggtg
    45961 acggtgtcaa tgacgcgcca gctctgaaaa cagccgatat cggtatcggt atgggaatca
    46021 ctggtacaga ggtttctaag ggggcttctg atatgattct tgcagatgat aactttgcga
    46081 ctattatcgt cgcagtggaa gaaggacgta aggtcttctc aaacattcaa aagactattc
    46141 agtacctact ttctgctaat actgctgaag tattaaccat cttcctatca accttgtttg
    46201 gttgggatgt cttacagccg gttcatcttt tgtggatcaa cttggtaacg gataccttcc
    46261 cagctatcgc tcttggtgtt gaacctgcgg aacctggtgt catgaatcat aaaccacgtg
    46321 gacgcaaggc aagcttcttc tcaggtggtg ttttgagttc tatcatttat caaggtgtac
    46381 tccaagcagc tcttgttatg agtgtttatg gccttgcgat tgcttaccca gttcatgtgg
    46441 gtgacaatca tgctattcat gcagatgccc taacgatggc ctttgcaacc cttggtttga
    46501 ttcagctctt ccatgcctac aatgtcaagt ctgtctacca atccatcttg acagttggcc
    46561 cattcaagtc taagaccttt aactggtcca tcttggtatc ctttatcctt ctcatggcaa
    46621 caatcgttgt agaaccgctt gaaggaatct tccacgtaac caaactagac ttgtcacaat
    46681 ggggaattgt tatggctgga agcttctcaa tgattatcat cgtcgaaatc gttaagttta
    46741 ttcaacgcaa actcggtttt gacaagaatg cgatttaagg gaggtttcgg atgtcagcat
    46801 atcaattacc gaccgtatgg caggatgaag ctagtaatca aggagctttt acggggctaa
    46861 acagaccaac agcaggtgcc cgtttcgaac aaaacttgcc aaaaggagaa caagcttttc
    46921 agctttattc actgggaaca ccaaatggtg tgaaggttac tatcttattg gaagaattac
    46981 tagaagctgg ttttaaggaa gcggcttacg acttgtataa gattgctatc atggatgggg
    47041 atcaattcgg ctcagacttt gtgaagctca atccaaattc caagattcca gccttattgg
    47101 accagtcagg tactgaaaac gtaagagtct ttgagtctgc tcatattctt ctttaccttg
    47161 ctgagaaatt tggagccttt ttaccaagta atcctgtgga aaaggtagaa gttttgaatt
    47221 ggctattctg gcaagcaggt gcagcacctt ttctaggtgg gggatttgga catttcttca
    47281 attatgctcc tgaaaaattg gaatatccta ttaaccgttt tacgatggaa gtgaaacgcc
    47341 agttggattt attggataag gaattggctc agaaacctta tattgcaggc aatgactata
    47401 cgattgcaga tattgctatc tggtcttggt atggacagtt agttcaagga aatctttacc
    47461 aaggttctgc aaaattcttg gatgcctcaa gttatcaaaa tctagtaaaa tgggcagaaa
    47521 aaattgccaa tcgtccagct gttaagcgtg gcttggaagt aacttataca gaaattaaat
    47581 agcaaaaaag tcatcttcgg atggcttttt gctacagttg agggaaaggg tttgcagtta
    47641 tcggcttttg tgctataatt gctataatat agtaaaaagc aagaggagtg tgaggaagtg
    47701 gaaaagaaaa ttgtgaagga tatcttattt ttatctcaag tgtctcagcc ggcaagtcag
    47761 gaggaccttt atcttgccag agatttgcag gatacactct tagcaaatcg tgatacctgt
    47821 gttggtctag ctgccaatat gattggggtg cagaagcgcg tgattatctt taatcttggc
    47881 ttggttcccg tggtcatgtt taacccagtg cttctgtcct ttgaaggatc ctatgaggca
    47941 gaagaaggct gtttgtcctt ggtaggtgtg agatcaacta agcgttatga aaccataagg
    48001 gttgcctatc gtgacagcaa gtggcaggaa cagaccatta ccttgacagg cttcccagct
    48061 cagatttgcc agcatgagct ggatcacttg gaaggacgaa tcatttagga ggaaagcaaa
    48121 tgaaacgaat agtctttgaa cttattttta tcgcaacgac ctggtatatc tttttaccgc
    48181 cccttaacct gaccagctgg gaatttctct tcttcctctg tgggcatttg ttagttgtgg
    48241 caatcttatt tggctttggc aaggggataa accttgtcaa aacggttcat gtgcgccacg
    48301 gtaaggcgga agctgcctta aatcttgagg gtttcaaaat caatcggtta gggaaaattc
    48361 tgttagcttc gattggagga attcttctct tggcagcttt ggtttccttg gtaacttcca
    48421 gcatgtttca ggctaaaaat tatgccaatg tagtcacggt tacggaaaaa gactttactg
    48481 aatttcctaa gagtgacacc agtaaggttc ctatcctaga tagaagtact gctgaaaaaa
    48541 ttggagaccg ctacttgggt tccctaaccg ataaggtgtc gcaatacgta gcggcagata
    48601 cctataccca attgacaatt gatgggaaac cttatcgggt cacaccacta gaatatgcag
    48661 accctatcaa atggtttaac aatcaagcca agggaatcgg tgagtatatc aaggtagaca
    48721 tggtaactgg aaatgcggat ttggtggact tgaagacacc aatcaagtat tcagactcgg
    48781 agtattttaa ccgtgatgtc aaacgtcacc tgcgcttgaa gtacccgacc aaaatcttta
    48841 aaactccatc ttttgaggtg aacgatgagg gcaatccttt ctatgtagca acggtttacc
    48901 aaaagcaatt tggacttgcg gttcctcgtc ctgcttcagt cattatcttg gatgctacaa
    48961 atggagaaac caaggaatac agcttatcag atgttccaga atgggtggac aggatctatc
    49021 cagcagagga aaccattgag caaatcaact acaacggcaa gtacaaggac ggtttcttga
    49081 atgccatgat ttccaagaaa aacgtgaccc agactaccaa tggctataat tacttgtcta
    49141 tcggtaatga catctatctc tacacaggtg tgacgtcggc taatgcggat gagagtaatc
    49201 ttggtttcat ccttgaaaat atgcgaacag gagaaattac taagtatagc ttggcttctg
    49261 cgacagaaga atcagcccgt gaatcagcag aaggtgctgt tcaggagaaa tcctacaaag
    49321 caaccttccc aatcctcatc aacctcaatg acaagcctct ctacatcatg ggcttaaagg
    49381 acaatgctgg cttggtcaaa gagtacgccc tggtagacgc agtcgagtac caaaatgtta
    49441 tcgttgctac tacagtggaa gagatgctca gcaagtatgc caataaaaac gaccttgaaa
    49501 ttgacaatgc aacgacagaa agcatcaagg gagtagtagc agacctcaaa tcagctgtta
    49561 tcaagggaga cactgtctac ttctttaaag ttgatggcaa gatatacaag gtaaaggctt
    49621 cagtatccga tgaccttcct taccttgaaa atggtaaaac cttcgaaggt caagtaggaa
    49681 aagacaatta tctcaagacc tttaaagtac aataatactc ttcgaaaatc tctagctcta
    49741 tctgcaacct caaaacagtg ttttgagcag cctgcggcta gtttcctagt ttgctctttg
    49801 attttcattg agtataaaaa aggtttattt aataaatata tgttataata aggtaaatta
    49861 agcctaatgg aggacaaaga aatgggtttt tatttgatgg ttgcttcaat gctactcgga
    49921 ctgctcgctt tgaagatagg tttttctcag ttcaaggaga aaaaagataa attcttatct
    49981 atcttaacaa gtttagctgg catagccctt gttctcgcgg ctgtctggtt aggatggccc
    50041 aaataaatag aaaaacctct gtcaaaccga ggtttttcaa atgaatttct tggttgtggc
    50101 taaaaaatat gctacactat caatatgaaa attttaatcc caacagcaaa agaaatgaac
    50161 acagacttgc caagtatcga ggcaattcct ttaaaaccag aaagtcagac cgtgcttgat
    50221 gccttggctc tctattctgc cagtcaattg gagagtttct acaaggtatc agctgagaaa
    50281 gcggcggaag aatttcaaaa tatccaagct ttgaaaaggc aaactgctca acactatcca
    50341 gccttgaaac tttttgatgg gcttatgtac cgcaacatta agagagataa gctgaccgag
    50401 gcggaacaag attatcttga aaatcatgtc ttcattacct cggctttgta cggtgttgtt
    50461 ccagccttgt cacccatggc tcctcaccgt ttggattttt tgatgaaatt aaaagtcgct
    50521 ggtaagactt tgaagagcca ttggaaggca gtctatgatg aagctctgaa gaaggaagaa
    50581 gtgattttct ctctcttgtc atcagagttt gagactgtat tttctaagga aatcagagca
    50641 aagatggtga ccttcaaatt catggaggat agaggcggtc agctgaagat tcactcaact
    50701 atctccaaga aagcgcgcgg ggcctttcta acagctttaa tagaaaatca agtacaaact
    50761 gtgggggaag cacgtcgctt gaactttgct ggatttgttt accgagaaga tttgtcacaa
    50821 ccacagggat tggtttttgt aaaggaagta tagaaatcag ataagagaaa ag
//
LOCUS       DBEROU010000010        45438 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.010, whole genome shotgun sequence.
ACCESSION   DBEROU010000010 DBEROU010000000
VERSION     DBEROU010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 45438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45438
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.010"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..546)
                     /locus_tag="ACL8CL_000623"
                     /pseudo
     CDS             complement(1..546)
                     /locus_tag="ACL8CL_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            523..795
                     /locus_tag="ACL8CL_000624"
     CDS             523..795
                     /locus_tag="ACL8CL_000624"
                     /inference="COORDINATES: protein motif:HMM:NF036719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGS1329921.1"
                     /translation="MFTCFIIHRTTIPYFVSQEVYWKVRNIEAEAIRRNCERGAIFSG
                     KIKYHEDSQFKGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            989..2323
                     /gene="trmFO"
                     /locus_tag="ACL8CL_000625"
     CDS             989..2323
                     /gene="trmFO"
                     /locus_tag="ACL8CL_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HGS1329922.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNNGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKEESEVIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            2407..3144
                     /gene="pyrH"
                     /locus_tag="ACL8CL_000626"
     CDS             2407..3144
                     /gene="pyrH"
                     /locus_tag="ACL8CL_000626"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HGS1329923.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            3153..3710
                     /gene="frr"
                     /locus_tag="ACL8CL_000627"
     CDS             3153..3710
                     /gene="frr"
                     /locus_tag="ACL8CL_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HGS1329924.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAVRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            3770..4624
                     /gene="cvfB"
                     /locus_tag="ACL8CL_000628"
     CDS             3770..4624
                     /gene="cvfB"
                     /locus_tag="ACL8CL_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HGS1329925.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKFRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            4633..4848
                     /locus_tag="ACL8CL_000629"
     CDS             4633..4848
                     /locus_tag="ACL8CL_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HGS1329926.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            4934..5902
                     /locus_tag="ACL8CL_000630"
     CDS             4934..5902
                     /locus_tag="ACL8CL_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HGS1329927.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEYSTEVAHD"
     gene            6095..6595
                     /locus_tag="ACL8CL_000631"
     CDS             6095..6595
                     /locus_tag="ACL8CL_000631"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1329928.1"
                     /translation="MIDQLSKYYSCRILTEKDIPSILSLYESNPLYFQHCPPEPNFAT
                     VKEDMLCLPEGKAKADKFFVGFWNGSDLVAVMDFVYAYPDEETVFIGLFMVDQAYQRK
                     GIGSHIVTEALAYFAKNFRKARLAYVKGNPQSQHFWEKQGFKSIGCEVKQELYTVVIA
                     EQSLED"
     gene            6598..6924
                     /locus_tag="ACL8CL_000632"
                     /pseudo
     CDS             6598..6924
                     /locus_tag="ACL8CL_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035616.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
     gene            complement(7225..8336)
                     /gene="ald"
                     /locus_tag="ACL8CL_000633"
                     /pseudo
     CDS             complement(7225..8336)
                     /gene="ald"
                     /locus_tag="ACL8CL_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003018852.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
     gene            8513..8936
                     /locus_tag="ACL8CL_000634"
                     /pseudo
     CDS             8513..8936
                     /locus_tag="ACL8CL_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            9004..9654
                     /locus_tag="ACL8CL_000635"
     CDS             9004..9654
                     /locus_tag="ACL8CL_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGS1329929.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDSSTGKEVNKEEKEEPVEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGSGTASSTSKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKKVSGIGAKTIEKLKDYVTVD"
     gene            9638..11878
                     /locus_tag="ACL8CL_000636"
     CDS             9638..11878
                     /locus_tag="ACL8CL_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HGS1329930.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSTSYLALLGFVFLLVCL
                     FFQFPWKSASKVLVICGIFGFWFVFQNWQQSQASQNLADSVEGVRILPDTIKVNGDSL
                     SFRGKSNGRAFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQGIYQTLNIKTIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPGPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLIAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWLLILLLI
                     SLALVYDLRKNIKGLTVLSLLITGLFFLTKYPLENEITMLDVGQGESIFLRDVTGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKENVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKHYPDLKVNVLKASQHGNKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            12057..12245
                     /locus_tag="ACL8CL_000637"
     CDS             12057..12245
                     /locus_tag="ACL8CL_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329931.1"
                     /translation="MTVTKSYKYDWNTVWEYSTNYHDHQYAWIPSWSRYDSYSEYKVG
                     GGWNYGRYEVINYYSGGY"
     gene            12277..12864
                     /locus_tag="ACL8CL_000638"
     CDS             12277..12864
                     /locus_tag="ACL8CL_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1329932.1"
                     /translation="MLNITHVTLKTRQVILQDVDFTFKKGRVYGLLAINGSGKTTLFR
                     AISNLIPISSGNISAPPSLFYYESIEWLDGNLSGMDYLRLIKNIWKSGLNLRDEIAYW
                     EMSDYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            12868..14048
                     /locus_tag="ACL8CL_000639"
                     /pseudo
     CDS             12868..14048
                     /locus_tag="ACL8CL_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     misc_feature    14208..14339
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            14355..14885
                     /gene="infC"
                     /locus_tag="ACL8CL_000640"
     CDS             14355..14885
                     /gene="infC"
                     /locus_tag="ACL8CL_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HGS1329933.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     gene            14918..15118
                     /gene="rpmI"
                     /locus_tag="ACL8CL_000641"
     CDS             14918..15118
                     /gene="rpmI"
                     /locus_tag="ACL8CL_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HGS1329934.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            15170..15529
                     /gene="rplT"
                     /locus_tag="ACL8CL_000642"
     CDS             15170..15529
                     /gene="rplT"
                     /locus_tag="ACL8CL_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HGS1329935.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            15587..15967
                     /locus_tag="ACL8CL_000643"
     CDS             15587..15967
                     /locus_tag="ACL8CL_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1329936.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            16217..17017
                     /locus_tag="ACL8CL_000644"
     CDS             16217..17017
                     /locus_tag="ACL8CL_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HGS1329937.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMVEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            17028..17966
                     /locus_tag="ACL8CL_000645"
     CDS             17028..17966
                     /locus_tag="ACL8CL_000645"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HGS1329938.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFVFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            18008..20041
                     /locus_tag="ACL8CL_000646"
     CDS             18008..20041
                     /locus_tag="ACL8CL_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HGS1329939.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYADNEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGVFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGAKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMAANEWIWDKESWFYLKFDGKIAEKEWVYDS
                     HSQAWYYFKSGGYMTANEWIWDKESWFYLKSDGKIAEKEWVYDSHSQAWYYFKSGGYM
                     TANEWIWDKESWFYLKSDGKMAEKEWVYDSHSQAWYYFKSGGYMAKNETVDGYQLGSD
                     GKWLGGKATNENAAYYQVVPVTANVYDSDGEKLSYISQGSVVWLDKDRKSDDKRLAIT
                     ISGLSGYMKTEDLQALDASKDFIPYYESDGHRFYHYVAQNASIPVASHLSDMEVGKKY
                     YSADGLHFDGFKLENPFLFKDLTEATNYSAEELDKVFSLLNINNSLLENKGATFKEAE
                     EHYHINALYLLAHSALESNWGRSKIAKDKNNFFGITAYDTTPYLSAKTFDDVDKGILG
                     ATKWIKENYIDRGRTFLGNKASGMNVEYASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(20102..21757)
                     /gene="pavA"
                     /locus_tag="ACL8CL_000647"
     CDS             complement(20102..21757)
                     /gene="pavA"
                     /locus_tag="ACL8CL_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HGS1329940.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            21862..22359
                     /gene="ybeY"
                     /locus_tag="ACL8CL_000648"
     CDS             21862..22359
                     /gene="ybeY"
                     /locus_tag="ACL8CL_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HGS1329941.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAKKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            22340..22735
                     /locus_tag="ACL8CL_000649"
     CDS             22340..22735
                     /locus_tag="ACL8CL_000649"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGS1329942.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFAITGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            22752..23651
                     /gene="era"
                     /locus_tag="ACL8CL_000650"
     CDS             22752..23651
                     /gene="era"
                     /locus_tag="ACL8CL_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HGS1329943.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            23700..24524
                     /gene="mutM"
                     /locus_tag="ACL8CL_000651"
     CDS             23700..24524
                     /gene="mutM"
                     /locus_tag="ACL8CL_000651"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HGS1329944.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFYLTDKVLISHLRMEGKYFYYPDQGPERKHAHVFFHFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            24524..25129
                     /gene="coaE"
                     /locus_tag="ACL8CL_000652"
     CDS             24524..25129
                     /gene="coaE"
                     /locus_tag="ACL8CL_000652"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HGS1329945.1"
                     /translation="MRKIIGITGGIASGKSTVTNFLRQQGFQVVDADAVVHQLQKSGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSKQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            25116..26315
                     /locus_tag="ACL8CL_000653"
     CDS             25116..26315
                     /locus_tag="ACL8CL_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HGS1329946.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYLVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            26315..26464
                     /gene="rpmG"
                     /locus_tag="ACL8CL_000654"
     CDS             26315..26464
                     /gene="rpmG"
                     /locus_tag="ACL8CL_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS1329947.1"
                     /translation="MRVKINLKCSSCDSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            26504..26737
                     /gene="secG"
                     /locus_tag="ACL8CL_000655"
     CDS             26504..26737
                     /gene="secG"
                     /locus_tag="ACL8CL_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HGS1329948.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            26839..29193
                     /gene="rnr"
                     /locus_tag="ACL8CL_000656"
     CDS             26839..29193
                     /gene="rnr"
                     /locus_tag="ACL8CL_000656"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HGS1329949.1"
                     /translation="MKDRIKEYLQDKGKVTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEAKKKHEITLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTIIQTVIKTSFRMTYSDVNDILAVDEEKRKEYHK
                     IVSSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSHSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKPFYKEVAKKGAKHGKGRGKGRRTK"
     gene            29156..29623
                     /gene="smpB"
                     /locus_tag="ACL8CL_000657"
     CDS             29156..29623
                     /gene="smpB"
                     /locus_tag="ACL8CL_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HGS1329950.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            29639..30499
                     /gene="tehB"
                     /locus_tag="ACL8CL_000658"
     CDS             29639..30499
                     /gene="tehB"
                     /locus_tag="ACL8CL_000658"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HGS1329951.1"
                     /translation="MEKLVAYKRMPLWNKQTMPEAVQQKHNTKVGTWGKITVLKGALK
                     FIELTEEGEVLAEHLFEAGADNPMAQPQAWHRVEAATDDVEWYLEFYCKPEDYFAKKY
                     NTNPVHSEVLEAMQTVKQGKALDLGCGQGRNSLFLAQQDFDVTAVDQNGLALEILQSI
                     VEQEDLDMPVGLYDINSASIEQEYDFIVSTVVLMFLQADRIPAIIQNMQEKTSVGGYN
                     LIVCAMDTEDYPCSVNFPFTFKEGELADYYKDWELVKYNENPGHLHRRDENGNRIQLR
                     FATLLAKKIK"
     gene            30572..31525
                     /gene="coiA"
                     /locus_tag="ACL8CL_000659"
     CDS             30572..31525
                     /gene="coiA"
                     /locus_tag="ACL8CL_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HGS1329952.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHIANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGKKLWLKERLTCLQQGFLYFSQNMGF
                     YVWELDKEKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIK
                     QDLTSYYQHFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            31544..33346
                     /gene="pepF"
                     /locus_tag="ACL8CL_000660"
     CDS             31544..33346
                     /gene="pepF"
                     /locus_tag="ACL8CL_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGS1329953.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQMEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYAYLNQEYYGLSKEDNPEIQYEWA
                     RIPHFYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            33348..34061
                     /locus_tag="ACL8CL_000661"
     CDS             33348..34061
                     /locus_tag="ACL8CL_000661"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HGS1329954.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGFLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            34128..35069
                     /gene="prsA"
                     /locus_tag="ACL8CL_000662"
     CDS             34128..35069
                     /gene="prsA"
                     /locus_tag="ACL8CL_000662"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HGS1329955.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(35188..35529)
                     /locus_tag="ACL8CL_000663"
                     /pseudo
     CDS             complement(35188..35529)
                     /locus_tag="ACL8CL_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            35948..36043
                     /locus_tag="ACL8CL_000664"
                     /pseudo
     CDS             35948..36043
                     /locus_tag="ACL8CL_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(36296..36916)
                     /locus_tag="ACL8CL_000665"
     CDS             complement(36296..36916)
                     /locus_tag="ACL8CL_000665"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS1329956.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPGSIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPAERILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(36916..37398)
                     /locus_tag="ACL8CL_000666"
     CDS             complement(36916..37398)
                     /locus_tag="ACL8CL_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HGS1329957.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKMSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(37398..38240)
                     /locus_tag="ACL8CL_000667"
     CDS             complement(37398..38240)
                     /locus_tag="ACL8CL_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HGS1329958.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDLLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(38335..39135)
                     /locus_tag="ACL8CL_000668"
     CDS             complement(38335..39135)
                     /locus_tag="ACL8CL_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HGS1329959.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPFYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            39279..40658
                     /gene="glmU"
                     /locus_tag="ACL8CL_000669"
     CDS             39279..40658
                     /gene="glmU"
                     /locus_tag="ACL8CL_000669"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002899862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HGS1329960.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIASEVQIEA
                     NVILKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVIVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGNNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            40668..41213
                     /locus_tag="ACL8CL_000670"
     CDS             40668..41213
                     /locus_tag="ACL8CL_000670"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS1329961.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            41225..41539
                     /gene="macP"
                     /locus_tag="ACL8CL_000671"
     CDS             41225..41539
                     /gene="macP"
                     /locus_tag="ACL8CL_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall synthase accessory phosphoprotein
                     MacP"
                     /protein_id="HGS1329962.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            41556..42248
                     /locus_tag="ACL8CL_000672"
     CDS             41556..42248
                     /locus_tag="ACL8CL_000672"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HGS1329963.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLTFLKALD"
     gene            42325..42816
                     /gene="rocS"
                     /locus_tag="ACL8CL_000673"
     CDS             42325..42816
                     /gene="rocS"
                     /locus_tag="ACL8CL_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HGS1329964.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            42914..43504
                     /locus_tag="ACL8CL_000674"
     CDS             42914..43504
                     /locus_tag="ACL8CL_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HGS1329965.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            43513..45276
                     /locus_tag="ACL8CL_000675"
     CDS             43513..45276
                     /locus_tag="ACL8CL_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HGS1329966.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINFLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFEFMKKLTGAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDKYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAKLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT        13737 a         8318 c        10095 g        13288 t
ORIGIN      
        1 gccaagattt taccagcttg tccgatattt ctgcaactca ttttgaacaa cttcatatca
       61 tgactatagt tcacagcgat atccaaagaa acaattctcc cttgacttgt gacaatcgct
      121 tgagccttca tagcgtgaaa tttcttttta ccagaatgat tcgctaattc tttttttagg
      181 gcgattgatt tgtacttccg tcgcatcaat cattaccgtg tcctcagaac tgagaggagt
      241 tcttgaaatc gtaacaccac tttgaacaag agttacttca acccattggc tccgacggat
      301 aaagttgctt tcgtgaatac caaaatcagc cgcaatttgt tcataagttc gatattctcg
      361 cacatattga agagtggcca taagaaggtc ttctaggctt aatttaggtt ttcgtccacc
      421 ttttgcgtgt ttaagttgat aagctgtttt taatacagct aatatctctt caaaagtcgt
      481 gcgctgaaca ccaacaagac gcttaaatcg tgcatcagtt agttgtttac ttgcttcatc
      541 attcatagaa ctactatacc atattttgtt tcgcaggaag tctattggaa agtaagaaat
      601 attgaagctg aggctattag aagaaattgt gagcgtggtg ctattttttc aggtaaaata
      661 aaatatcacg aagattcaca gtttaaagga gatcactatg ttgaatgtta tgctgtttta
      721 gataatacgg ttatagcaag agatagaata acagtcccta tcgatccgtt atgtggaaaa
      781 gattttatag agtagcatat aattgattct taactggaat actcactatc tctttacatc
      841 aagaaaatga ctaaacaggg aagtttgcct tcttcccttt ttttgttata ctagtagaag
      901 aaaaaattag aaagatttgt gggtgtcaaa cagcccagtg gggtgtttta atatggactt
      961 aggtcccacc caaagaggta ttagtgtcgt gtctcaatct tatatcaatg ttatcggtgc
     1021 tggtttggca ggttctgaag cagcttacca aatcgcagag cgtggtattc cagttaaact
     1081 atatgaaatg cgtggtgtca agtctacacc ccagcataaa acagacaatt ttgctgagtt
     1141 ggtttgttcc aattctttgc gtggggatgc tttgacaaat gcagttggtc ttctcaagga
     1201 agaaatgcgt cgcttgggtt ctgttatctt ggaatctgct gaggctacac gtgttcctgc
     1261 aggtggtgcc cttgcagtgg accgtgatgg tttctctcaa atggtgaccg aaaaagttgc
     1321 caaccacccc ttgattgaag tggttcgtga tgaaattaca gaattgccga cagatgttat
     1381 tacggttatc gctactggtc ctttgacaag tgatgccttg gctgaaaaga ttcatgccct
     1441 taataatggc gatggcttct atttctacga tgcagcagcg cctattatcg atgtcaacac
     1501 tatcgatatg agtaaggtct atctcaaatc tcgttatgat aagggagaag cagcctatct
     1561 caatgctcca atgaccaagc aagagtttat ggatttccat gaagccttgg tcaatgcgga
     1621 agaagcaccg ctcaattctt ttgaaaaaga aaagtacttt gaaggatgta tgccaatcga
     1681 agttatggcc aaacgtggca ttaaaactat gctttatggc cctatgaagc cagtcggtct
     1741 tgagtatccg gacgactaca caggacctcg tgatggagaa tttaaaacac cttatgcggt
     1801 tgtgcaactt cgtcaggata atgcagctgg tagcctatac aatattgttg gtttccagac
     1861 ccacctcaaa tggggagaac aaaagcgtgt cttccaaatg attccgggtc ttgaaaatgc
     1921 ggagtttgtc cgttatggtg tcatgcatcg caattcttac atggattcac caaatcttct
     1981 tgagcagact taccgttcca agaaacaacc aaatctcttc tttgctggtc agatgacggg
     2041 ggtggaaggc tatgttgagt cggcggcttc gggcttagtt gcgggaatta acgcagctcg
     2101 tctcttcaag gaagaaagcg aggttatttt ccccgagacg acagcgattg gaagcttagc
     2161 tcattacatt acccatgccg acagcaaaca tttccaacca atgaatgtca attttgggat
     2221 catcaaggag ttggaaggcg agcgtatccg tgataagaag gctcgttatg aaaaaattgc
     2281 agagcgtgcc cttgccgact tagaggaatt tttgactgtc taattttttt gaaagaattg
     2341 ttcatgatac tataaaaatc ttagaaattg tgataaaata ggtaggatga aagaaggaga
     2401 gtgaaaatgg cgaatcccaa gtataaacgt attttaatca agttatcagg tgaagccctt
     2461 gccggtgaac gtggcgtagg gattgatatc caaacagttc aaacaatcgc aaaagagatt
     2521 caagaagttc atagcttagg tatcgaaatt gcccttgtta ttggtggagg aaatctctgg
     2581 cgtggagaac ctgcagcaga agcaggtatg gaccgtgttc aggcagatta cactggaatg
     2641 cttgggactg ttatgaatgc tcttgtgatg gcagattcat tgcaacaagt tggggttgat
     2701 acgcgtgtac aaacagctat tgctatgcaa caagtggcag agccttatgt ccgtggacgt
     2761 gcccttcgtc accttgaaaa aggccgtatc gttatctttg gtgctggaat tggttcacca
     2821 tacttctcga cagatacaac agcggccctt cgtgcagctg aaatcgaagc agatgccatc
     2881 ctcatggcta aaaatggcgt cgatggtgtg tacaatgccg atcctaagaa ggacaagaca
     2941 gccgttaagt ttgaagaatt gacccaccgt gatgttatca acaaaggtct tcgtatcatg
     3001 gactcaacag cctcaaccct ctcaatggac aacgacattg acttggttgt cttcaacatg
     3061 aaccaatcag gcaacatcaa acgtgtcgta tttggtgaaa atatcggaac aacagtttca
     3121 aataatatcg aagaaaagga ataagaaaga atatggctaa cgtaattatt gaaaaagcta
     3181 aagagagaat gacccagtct caccaatcac ttgctcgtga atttggtggt atccgtgctg
     3241 gtcgtgccaa tgcaagcttg cttgaccgtg tacatgtaga atactatgga gtcgaaactc
     3301 ctcttaacca aatcgcttca attacgattc cagaagcgcg tgttttgttg gtaacaccat
     3361 ttgacaagtc ttcattgaaa gacatcgaac gtgccttgaa cgcttctgat cttggtatca
     3421 caccggctaa tgacggttct gtgattcgct tggttatccc agctcttaca gaagaaactc
     3481 gtcgtgacct tgctaaagaa gtgaagaagg tcggcgaaaa tgctaaagtg gctgtccgca
     3541 atatccgtcg cgatgctatg gacgaagcta agaaacaaga aaaagcaaaa gaaatcactg
     3601 aagacgaatt gaagactctt gaaaaagaca ttcaaaaagt aacagacgat gctgttaaac
     3661 acatcgacga catgactgct aacaaagaga aagaactttt ggaagtctaa aaataaacag
     3721 aaaaactcag ttggcattgc tggctgagtt ttattcgaaa gaaggaaata tgaatacaaa
     3781 tcttgcaagt tttatcgttg gactgatcat cgatgaaaac gaccgttttt actttgtgca
     3841 aaaggatggt caaacctatg ctcttgctaa ggaagaaggc caacatacag taggggatac
     3901 ggtcaaaggt tttgcataca cggatatgaa gcaaaaattc cgcctgacaa ccttagaagt
     3961 gactgccact caggaccaat ttggttgggg acgtgtcaca gaggttcgta aggacttggg
     4021 tgtctttgtg gatacaggcc ttcctgacaa ggaaatcgtt gtgtcactcg atattctccc
     4081 tgagctcaag gaactctggc ctaagaaggg cgaccaactc tacatccgtc ttgaagtaga
     4141 taagaaagac cgtatctggg gcctcttggc ttatcaagaa gacttccaac gtcttgctcg
     4201 tcctgcttac aacaacatgc agaaccaaaa ctggccagcc attgtttacc gtctcaagct
     4261 gtcaggaact tttgtttacc taccagaaaa taatatgctt ggttttattc atcctagcga
     4321 gcgttacgca gagccacgtt tggggcaagt attagatgcg cgcgttattg gtttccgtga
     4381 agtggaccgc actctgaacc tctccctcaa accacgctcc tttgaaatgt tggaaaacga
     4441 tgctcagatg attttgactt atttggaaag caatggcggt ttcatgacct taaatgacaa
     4501 gtcatctcca gacgacatca aggcaacctt tggcatttct aaaggtcagt tcaagaaagc
     4561 tttaggtggt cttatgaagg ctggtaaaat caagcaagac cagtttggga cagagttgat
     4621 ttagggaggc ttatgagaaa atcattttac acttggctca tgaccgagcg caatcctaaa
     4681 agtaacagtc ccaaagcaat tttggcagac ctcgcttttg aagaggcagc ttttccaaaa
     4741 catacagatg attttgatga ggtcagtcgc tttttggagg agcatgccag tttctctttt
     4801 aacctaggag attttgacag catttggcag gaatatctag aacactagca tttattcatt
     4861 gggtttgggc tagtaatttc tccatccctc tgctataata aaaagaaata aaaggattag
     4921 agaggttctt tatttgaagg aacattcaat agacattcaa ctgagtcatc cagatgacct
     4981 gtttcatctt tttggttcca atgaacgcca tcttcgtttg atggaagaag agcttgatgt
     5041 tgtgattcat gctcgtacgg agattgtcca ggttttggga gaagagtctg cctgtgagga
     5101 agcccgtcaa gttattcagg ctttgatggt cttggtaaat cgtgggatga ccgttggtac
     5161 gccagatgta gtcactgcga ttagcatggt caaaaacgat gaaattgata agtttgtcgc
     5221 cctttacgaa gaagaaatta tcaaggataa tactgggaaa ccgatccgcg tcaaaacctt
     5281 aggtcaaaaa ctttatgtgg acagtgtcaa acagcatgat gtgacctttg gaattgggcc
     5341 tgcagggacg gggaagacct ttcttgcagt tactttggca gtgactgccc ttaaacgtgg
     5401 gcaagtcaag cggattatct tgacacgtcc agcggtggaa gcaggagaga gtctaggttt
     5461 tcttccgggt gatcttaagg agaaggtaga tccttacctt cgtcctgttt acgatgcctt
     5521 gtatcagatt ctggggaaag accaaacgac tcgtctcatg gagcgtgaaa ttatcgaaat
     5581 tgcgcccctt gcctatatgc gtggtcggac cttggatgat gcctttgtca ttctcgatga
     5641 ggcgcaaaac acgaccatca tgcagatgaa gatgttcttg acgcgtttag gctttcattc
     5701 taagatgatt gtcaatggag atatcagtca gattgacctg ccacgcaatg tcaagtcagg
     5761 tttgattgat gcccaagaga aactcaagaa catccatcag attgactttg ttcatttttc
     5821 agctaaggat gtggttcgcc atccagttgt cgctcagatt atccgagctt atgaatattc
     5881 tactgaagtt gcacacgact gattttgagg aagttcgcct gcaaaagaat atacttttga
     5941 gagaggaaaa tccagttgta taggctaaag gttttatcca aaggtttgag acaacgatta
     6001 ggcacgatgg aaagaacttt tatgtggctg atgacgatca gtgcatcttc ctgtgtcata
     6061 atcacagggc acaagaaagt aggaatttga aaagatgatt gaccaactat ctaagtatta
     6121 cagttgtagg atactaactg aaaaggatat tccaagtatt ttatctttat atgaaagtaa
     6181 tcctctgtat tttcagcatt gtccaccaga accaaatttt gcaactgtaa aagaggacat
     6241 gctttgtcta cctgaaggta aagctaaggc tgataagttt tttgttggat tttggaatgg
     6301 atctgacctt gtggctgtta tggattttgt ctatgcatat cctgatgagg agactgtttt
     6361 tattggtttg tttatggttg atcaagccta tcagaggaaa gggattggta gtcatattgt
     6421 gacagaagca ctagcttatt ttgctaagaa ctttcgaaag gcacgtttgg cttatgttaa
     6481 gggaaatccg caatctcagc atttttggga aaagcagggc tttaaatcaa ttggatgcga
     6541 ggttaagcaa gaactctata cggttgttat cgctgaacag agcctagaag attagaaatg
     6601 gcatcaagta agaactattt ggaatttgtt ttggaacaat tatcaggatt agatgatgtg
     6661 acttaccgtt ccatgatggg ggagtatatt ctttacttcc gcggcaagat tattggcggc
     6721 atttatgacg atcgcttttt agttaaaccc gtgcaagcag tcttagataa gattgaccaa
     6781 tcttcttttg agtttccata caaaggtgcc aaagaaatga tttgagtgga agaacttgat
     6841 aataagatgt ttctataaga cctgatttta gctatgtata accaactgcc aacgcccaaa
     6901 cctaaaaaga aaaagcaagt gtgaacgaag taaaaaagaa gtctgctaag gccctgtctt
     6961 tgcacgggta aaattttata tataaaaaga agctgggact aaagagctca gcttcctttg
     7021 gtttatataa ttgtcattac aagacgaagt ggttgggcga aactctgttg actttattca
     7081 atttagagtt tcttatgcac gattgagtct ggaacgaaag tctccagttg caaagtatac
     7141 agtacaataa accaacgatg taatagctga tgacacaaag cacagtgggt aggacttgcg
     7201 aagtcaccct tttcttttca aaatttatac taaatcattg atatcagtgt agtcacgatt
     7261 aagtccttga gcaactggta ggctagtcaa gtaaccttga taagtggtca caccttgacg
     7321 caagccttca tcttcagaga ttgcttgtgc gaatcctttg ccagccaaag cttcgatata
     7381 aggaagagtg acattggtta gggcgatggt tgaagtgtgg gcaaccgcac cagggatatt
     7441 ggcaacggca tagtggagaa caccgtgttt ttcatagacg ggttcatcgt gcgttgtcac
     7501 acggtcagct gtttcgataa ggccaccttg gtcaacagca acgtcaacga tacagagcct
     7561 ggacgcattt gtttgaccat ctcatctgtc accaattccg gtgcttttgc accagggatg
     7621 agaatggctc caatcaccac atcagcatct ctcacacttg cttcaatgtt gaatgaatta
     7681 gatataagag tttgaatttg acttccaaag acttcttcta gaactgagag acgcttggca
     7741 ctaatatcta aaatagtcac ttgagcacca agaccaaggg cgatgcgggc agcatgtgta
     7801 ccgacgacac catcaccgat gatagttact tttccttttg gaacacctgg tacaccacca
     7861 ggtagaacac cagagccacc agcttgctta gtaaggaagt gagctccgat ttaaacagcc
     7921 atacgacctg caacctcact cataggaacg aggagcggta gttgtccttg attgtcacga
     7981 acagtttcat aggcaattcc tgttgttttt gctgctaaca tagcatctgc taattctgga
     8041 gcagcggcca tgtgcaagta ggtgaagaga agaagatcgt cgcgcaagta accgtattca
     8101 gaacttaaag gttcttttac tttcacaacc aactctgcta cccaagcttc accagcagta
     8161 gcgacaatct cagctccttg cttttgatag tcagcatcag taaagccaga accgagacca
     8221 gcatttgttt cgataaggac acgatgacca cgactaacta agctatgaac acctgcaggt
     8281 gtgagggcga cacggttttc gttattttta atttcttttg ggattccgat taacattgag
     8341 ataacctacc tttcaattga cggtcttgtt ttggttgtca cattccagtt cataaatcaa
     8401 aaatgtgacg gtttcattgt atatgaaacc gcttcaaaaa tcaagaaaaa cttgtcatcc
     8461 caattttttt atgctagact agtgaaaatc aagctctaat ggagggaaaa gtatggaatc
     8521 aatttttgta aaacttgccc agtatccgtc tatagaaacg gagcgtttat tgcttagacc
     8581 tgtaactttg gatgatgcgg aagcaatgtt tgactatgcc tcggacaagg gtaatacacg
     8641 ttacactttt ccaaccaatc aaagcttgga agaaaccaag aataacattg ctcagttcta
     8701 cttggctaat cccttgggac gttggggaat agaactaaaa agcaatggtc agtttattgg
     8761 aaccattgac ttgcacaagt aaggctaatc ccgcgtcagg aaaggtcatg gagaaatcag
     8821 gcatgcgttt ttcccatgca gaaccatatg cttgtatgga ccagcatgaa aaaggccgaa
     8881 tcgtgacaag agttcattat gtcttgacca aggaagacta ttttgcaaat aaataagcag
     8941 ttgaaaagaa atttttcgac tgttttttct tcctcttacg aataatctaa gagaggagaa
     9001 aatatggaag caattatcga gaaaatcaaa gagtataaaa tcatcgtcat ctgtactggt
     9061 ctgggcttgc ttgtaggagg atttttcctg ctaaagccag ctccacaaac acctgtcaaa
     9121 gagacgaatt tgcaggctga agttgcagct gtttccaagg actcatcgac cggaaaggaa
     9181 gtgaacaagg aagagaagga agaaccagtt gaacaagatc taatcacagt agatgtcaaa
     9241 ggtgctgtca aatcgccagg gatttatgac ttgcctgtag gtagtcgagt caatgatgct
     9301 gttcagaagg ctggtggctt gacagagcaa gcagacagca agtcgctcaa tctagctcag
     9361 aaagttagtg atgaggctct ggtttacgtt cctactaagg gagaagaagc agttagtcaa
     9421 cagactggtt cggggacagc ttcttcaaca agcaaggaaa agaaggtcaa tctcaacaag
     9481 gccagtctgg aagaactcaa gcaggtcaag ggactgggag gaaaacgagc tcaggacatt
     9541 attgaccatc gtgaggcaaa tggcaagttc aagtcagtag acgagctcaa gaaggtctct
     9601 ggcattggtg ccaagactat agaaaagtta aaagactatg ttacagtgga ttaagaattt
     9661 ctctattccc ctaatttacc tgagttttct gttactttgg ctttactacg ccattttttc
     9721 aacatcctat cttgctttgt tgggatttgt ttttctgcta gtctgtctct ttttccaatt
     9781 tccttggaaa tctgctagca aagttctagt gatttgtgga atctttggat tttggtttgt
     9841 ttttcaaaat tggcaacaga gtcaagcgag tcaaaatctg gcggattctg ttgaaggggt
     9901 acggattttg cctgatacta ttaaggttaa tggtgatagt ctatcctttc gtggcaagtc
     9961 taacggtcgt gctttccaag tctattataa actccagtcc gaggaggaga aagaagcctt
    10021 tcaagcttta accgacctgc atgagatagg actagaaggg aagctttcgg agccagaagg
    10081 gcagagaaat tttggtggct ttaattacca agcctatctg aagactcagg gaatttacca
    10141 gactctcaat atcaaaacaa tccagtcact tcaaaagatt ggcagttggg atataggaga
    10201 aaacttgtcc agtttacgtc gaaaggctgt ggtttggatt aagacgcact ttccaggccc
    10261 tatgcgcaat tacatgacag gactcttgct gggacatctg gacaccgact ttgaggagat
    10321 gaatgagctt tattccagtc taggaattat ccacctcttt gccctatctg gcatgcaggt
    10381 aggttttttc atgaatggat ttaagaaact tctcttgcga ttgggcttga cccaagaaaa
    10441 gttgaaatgg ctgacttatc ccttttccct tatctatgcg ggactaactg gattttcagc
    10501 atcggttatt cgcagtctct tgcaaaagct actggctcaa catggggtta agggcttgga
    10561 taattttgcc ttgacggtgc ttgtcctctt tattgtcatg ccaaactttt tcttgatagc
    10621 aggaggagtc ttgtcctgcg cttatgcttt tatcctgacc atgaccagca aagaagggga
    10681 ggggctcaag gctgttgcta gtgaaagtct agtcatctcc ttgggcatat tgcccattct
    10741 atccttctat tttgcggaat ttcaaccttg gtctatcctt ttgacctttg tcttttcctt
    10801 tctatttgac ttgaccttct taccgctctt gtctatttta tttgtccttt cctttctcta
    10861 tccagtcatt cagctgaact ttatctttga atggttagag ggcattattc gcttggtctc
    10921 gcaggtggca aggagaccgc ttgtctttgg tcaacccaac gcatggcttt taatcttatt
    10981 gttaatttcc ttggctttgg tctatgattt gaggaaaaac attaaaggat taacagtatt
    11041 gagtttattg attacaggtc tctttttcct taccaagtat ccactggaaa atgaaatcac
    11101 catgctggat gtggggcaag gagaaagtat tttcctacgg gatgtaactg ggaaaaccat
    11161 tctcatagat gtaggtggta aggcagaatc ttataagaaa atcgaaaaat ggcaagaaaa
    11221 gatgacgacc agcaatgccc agcgaacctt gattccctat ctcaaaagtc gaggagtagc
    11281 taagattgac cagctaattt tgactaacac ggacaaggag aatgttggag atttgtcaga
    11341 ggtgaccaag gctttccatg taggggagat tctagtatca aaagacagtc tgaaacagaa
    11401 ggaatttgtg gcagaactac aggcgactca aacaaaggtg cgtagtatga cagtagggga
    11461 gaacttgccc atttttggaa gtcagttaga agttctatct ccaaggaaaa tgggagatgg
    11521 aggacacgat gataccctag ttctgtatgg gaaattcttg gataagcaat ttctcttcac
    11581 gggaaatttg gaggagaaag gagagaagga cttgctgaag cactatccag acttgaaagt
    11641 aaatgttttg aaagctagcc aacatggcaa taaaaaatca tcaagtccag cctttctaga
    11701 aaaactcaaa ccagagctta ctcttatctc agttggaaag agcaatcgaa tgaaactccc
    11761 ccatcaggaa acattgacac gactggaagg tatcaatagc aaagtttatc gaactgacca
    11821 gcaaggagct atacgtttta aggggttgga tagttggaaa atcgaaagtg ttcgatagga
    11881 aggataaatg ttgtagatta gtgaaataaa ctaaaaattt gttgcataat aatgataaaa
    11941 atggtataat gaaaacgtat tcaatattga ggatgtaaaa tcattaaaaa ttagcaaaaa
    12001 tcgtttaatt agttagttta taatctattg gtcttcttca gtccagtgta tctgctgtga
    12061 cagtcactaa aagttacaag tatgattgga atacggtttg ggaatatagt accaactatc
    12121 acgaccatca gtatgcttgg attccgtcat ggtctcgtta tgacagctat tctgagtata
    12181 aagttggcgg aggctggaac tacggtcgtt atgaggtcat aaactattac agcggaggct
    12241 attaatcctt aaagagtgag aaaaggaggg ctggatatgt taaatattac tcatgttacc
    12301 ttaaaaacgc gacaagtcat cttgcaagat gtggatttca cctttaaaaa gggtagggtt
    12361 tatggtcttc ttgctatcaa tggctctgga aagacgaccc tgttccgtgc cattagcaat
    12421 ttaattccca taagtagtgg aaatatctca gcccctcctt ctttatttta ttatgagagt
    12481 attgaatggc tggatggaaa cttaagtggg atggactacc ttcgtcttat caaaaacatc
    12541 tggaagtcag gtctgaactt gagggatgaa atcgcctatt gggaaatgtc tgactatatc
    12601 agtcttccca ttcgcaagta ttccttaggc atgaagcaac gcttggtgat tgccatgtat
    12661 ttcctcagtc aggccaaatg ctggctcatg gatgagatta caaatggctt agatgagtat
    12721 tatcgacaga agttttttga taggttagca caaatcgata gacaagaaca gctggttctt
    12781 ttaagttccc actataagga agagttggtt gatatctgcg atagagtagt aaccattcat
    12841 caggggcaga tagaagaggt ttagtttatg aaagatgtta gtctattttt attgaaaaaa
    12901 gttttcaaaa gccgcttaaa ctggattatc ttagctttat ttgtatctgt actcggtgtt
    12961 actttttatt ttaatagtca gactgcaaac tcagtcagct tggagagaga gttggaaact
    13021 cgtcttgtag accgtgagag agtcatcaat gaatatgaag agaaactctc ccaaatgtct
    13081 gataccagct cggaggaata ccagtttgct aaaaataatt tagacgtgca aaaaaatctt
    13141 ttgacgcgaa agacagaaat tctaacttta ctaaaagaag ggcgctggaa agaggcctac
    13201 tatctgcagt ggcaagatga agagaaagat tatgaatttg tatcaaatga cccgactgct
    13261 agctctgaat taaaaatggg ggttgaccgc gaacggaaga tttatcaagc cctgtatccc
    13321 ttgaacataa aagcacatac tttggagttt ccgacccacg ggattgatca gattgtctgg
    13381 attttagagg ttatcatccc aagcttgttt gtggttgcga ttatttttat gctaacacaa
    13441 ctatttgcag aaagatatca aaatcatctg gacacagctc acttatatcc tgtttcaaaa
    13501 gtgacatttg caatatcctc tcttggagtt ggagtgggat atgtaactgt gctgtttatc
    13561 ggaatctgtg gcttttcttt tctagtggga agtctgataa gtggttttgg acagttagat
    13621 tatccctacc caatttatag cttagtgaat caagaagtaa ctattgggaa aatacaagat
    13681 gtattatttc ctggcttgct cttagctttc ttagccttta tcgtcattgt ggaagttgtg
    13741 tacttgattg cttacttttt caagcaaaaa atgcctgtcc tctttctttc actcattggg
    13801 attgttggct tattgtttgg catccaaaca ataagcctct tcaaaggatt gcacatctga
    13861 ttccctttac ttacttgcgt tcagtggaga ttttatctgg aagattatct aagcagattg
    13921 ataatgtcga tctaaattgg agcatgggga tggtcttact tccttgcctg attatccttt
    13981 tgttagtggg gattctattt atcgaaagat ggggaagttc acgtaaaaag gaagttttta
    14041 aagtttagtt ttcctatagg gaagctaaag aaaaactaac atagggagag aatcaacttg
    14101 gttctctctt tttgattcga aaaccaaacc aaaatacaaa cacaaacttt tcaaaaaata
    14161 actttttatc ttgacaagag ctagaaaact tggtatcata taaaagttga gaaaagcaga
    14221 agtgagagct tctcgccttg tgacattaag ttgcctggcc ctacggatga aaagtttcga
    14281 agaaacgcta tcataacgtg cgggcttgta tatttacgag tccgctattg tttttctcta
    14341 ataaaacaaa agaggtgaaa accatagcaa agcaagactt attcatcaat gatgagattc
    14401 gtgtacgtga agttcgcttg attggtcttg agggagaaca gctaggcatc aagccactca
    14461 gtgaagcgca agctttggct gataacgcta atgttgacct agtattgatt caaccccaag
    14521 ccaaaccgcc tgttgcaaaa attatggact acggtaagtt caaatttgag taccagaaga
    14581 agcaaaaaga acaacgtaaa aaacaaagcg ttgttactgt gaaagaagtt cgtctaagtc
    14641 caacaattga caagggtgac tttgatacaa aacttcgtaa tgcacgtaag ttccttgaaa
    14701 aaggaaataa agttaaggta tctattcgct ttaagggtcg tatgattacc cataaagaga
    14761 ttggtgcaaa agttttagcc gagtttgctg aagcaactca agatattgcc atcatcgaac
    14821 aacgtgctaa aatggatgga cgtcaaatgt tcatgcaatt ggcgccagcg actgacaaaa
    14881 aataattgtc agaaagttaa ataaaggaga aaaaatcatg ccaaaacaaa aaacacaccg
    14941 cgcatcagct aaacgtttca aacgtacagg ttctggtgga cttaaacgtt tccgtgctta
    15001 cacttctcac cgtttccacg gaaaaactaa gaaacaacgt cgtcatcttc gtaaagcatc
    15061 tatggtgcat tcaggagatt acaaacgtat caaagcaatg cttactcgct tgaaataata
    15121 gcttgactgt aagtaaacaa ttctaggaaa tatttggagg aaataaaaca tggcacgtgt
    15181 taaaggtggc gttgtatcac gcaaacgtcg taaacgtatt cttaaattag caaaaggtta
    15241 ctatggagct aaacacatct tgttccgtac tgcaaaagaa caagtaatga actcttacta
    15301 ctatgcatac cgtgaccgtc gtcagaaaaa acgtgacttc cgcaaattgt ggattactcg
    15361 tatcaatgcg gcagctcgta tgaacggact ttcatactca caattgatgc atggtttgaa
    15421 attggctgag atcgaagtta accgtaaaat gcttgctgac ttggctgtta acgatgcagt
    15481 agctttcaca gctcttgcag atgcagctaa agcaaaactt ggtaaataac aactgatgag
    15541 actggaacag gattgtccag tctttttaaa aataaaggag aaagatatgg cttcaaaaat
    15601 gctacacact tgcttacgag tagaaaatct tgaaaaatca atcgcatttt atcaagatgc
    15661 ttttggtttt aaagaattgc gtcgcagaga ttttccagac catgccttca cgattgtcta
    15721 tctaggtctt gagggtgatg attatgagtt ggagttgact tataactacg atcacggtcc
    15781 gtatgtggtt ggtgatgggt ttgcccatat cgccctcagt acacctgatc ttgaggcact
    15841 tcatcaagaa cacagtgcaa aaggctatga agtgactgag ccaaatggtc taccaggaac
    15901 tacaccaaac tattactttg ttaaagaccc tgatggatat aaggtcgaag tcattcgtga
    15961 aaaataattt cgtgcaagtt aagtagaata ttcgttttct acttgacttt ttttagctga
    16021 aagagtatac taataaaaat tcaaccttta aggggagtcc agagagactc acaaggtgtc
    16081 agataaaaga atagtgcaat tttctagagg agaccttttg agtgtgctct cttgtgttgt
    16141 acgattttaa ctgaggcctt gcactagcaa ggtcttttct ttatctggtc cccttaaaat
    16201 ttaaggagga aaagttatga atctcacatg taagaagcgt ttgggtgtca ttcggttgga
    16261 aaccatgaag gtggttgcac aagaggaaat cgcgccagca atctttgaat tagtcctaga
    16321 aggagaaatg gttgaagcca tgcgagcagg ccaatttctt catctgcgtg taccagacga
    16381 tgcccatctc ttacgtcgtc ctatttcaat ttcgtctatt gacaaggcaa acaagcagtg
    16441 tcacctcatt tatcggattg agggagctgg gactgcaatt ttttcaacct taagtcaggg
    16501 agacactctt gatgtgatgg ggcctcaggg aaatggtttt gacttgtctg accttgatga
    16561 gcagaatcag gttctccttg ttggtggtgg gattggtgtt ccacccttgc ttgaggtggc
    16621 caaggaattg catgaacgtg gagtgaaagt agtgacagtc ctcggttttg ctaataagga
    16681 tgctgttatt ttgaaaacgg aattggctca gtatggtcag gtctttgtaa cgacagatga
    16741 tggttcttat ggcatcaagg gaaatgtttc cgttgttatc aatgatttag atagtcagtt
    16801 tgatgctgtt tactcgtgtg gggctccagg aatgatgaag tatatcaatc aaacctttga
    16861 tgatcaccca agagcctatt tatctctgga atctcgtatg gcttgtggga tgggagcttg
    16921 ctatgcctgt gttctaaaag taccagaaag cgagacggtc agccaacgcg tctgtgaaga
    16981 tggtcctgtt ttccgcacag gaacagttgt attataagga gaaaattatg actacaaatc
    17041 gattacaagt gtctctacct ggtttggatt tgaaaaatcc gattattcca gcatcaggct
    17101 gttttgtctt tggacaagag tatgccaagt actatgattt agacctttta ggttctatta
    17161 tgatcaaggc gacaaccctt gaaccacgtt ttgggaatcc aactccaaga gtggcagaga
    17221 cgcctgctgg tatgctcaat gcaattggct tgcaaaatcc tggtttagag gttgttttgg
    17281 ctgaaaagct accttggctg gaaagagaat atccaaatct tcctattatt gccaatgtag
    17341 ctggtttttc aaaacaagag tatgcagctg tttctcatgg gatttccaag gcaactaatg
    17401 taaaagctat cgagctcaat atttcttgtc ccaatgttga ccactgtaat catggacttt
    17461 tgattggtca agatccagat ttggcttatg atgtggtgaa agcagctgtg gaagcctcag
    17521 aagtgccagt ttatgtcaaa ttaaccccga gtgtgaccga tatcgttact gtcgcaaaag
    17581 ctgcagaaga tgcgggagca agtggcttga ccatgatcaa tactctggtt ggaatgcgct
    17641 ttgacctcaa aaccagaaaa ccaatcttgg ccaatggaac aggtggaatg tcaggtccag
    17701 cagtctttcc agtagccctc aaactcatcc gccaagtagc ccaaacaaca gacctgccta
    17761 tcattggaat ggggggagtg gattcggctg aagctgccct agaaatgtat ctggctgggg
    17821 catctgctat cggagttgga acagctaact ttaccaatcc ttatgcctgc cctgacatca
    17881 tcgaaaattt accaaaagtc atggataaat acggtattag cagtctggaa gaactccgtc
    17941 aggaagtaaa agagtctctg aggtaaactg caatcaatct gttcttgatt ttttattagt
    18001 ttgtaatatg aatttaggag aattttggta caataaaata aataagaaca gaggaagaag
    18061 gttaatgaag aaagtaagat ttattttttt agctctgcta tttttcttag ctagtccaga
    18121 gggtgcaatg gctagtgatg gtacttggca aggaaaacag tatctgaaag aagatggcag
    18181 tcaagcagcg aatgagtggg tttttgatac tcattatcaa tcttggttct atataaaagc
    18241 agatgctaac tatgctgata atgaatggct aaagcaaggt gacgactatt tttacctcaa
    18301 atctggtggc tatatggcca aatcagaatg ggtagaagac aagggagtct tttattatct
    18361 tgaccaagat ggaaagatga aaagaaatgc ttgggtagga acttcctatg ttggtgcaac
    18421 aggtgccaaa gtaatagaag actgggtcta tgattctcaa tacgatgctt ggttttatat
    18481 caaagcagat ggacagcacg cagagaaaga atggctccaa attaaaggga aggactatta
    18541 tttcaaatcc ggtggttatc tactgacaag tcagtggatt aatcaagctt atgtgaatgc
    18601 tagtggtgcc aaagtgcagc aaggttggct ttttgacaaa caataccaat cttggtttta
    18661 catcaaagaa aatggaaact atgctgataa agaatggatt ttcgagaatg gtcactatta
    18721 ttatctaaaa tccggtggct acatggcagc caatgaatgg atttgggata aggaatcttg
    18781 gttttatctc aaatttgatg ggaaaatagc tgaaaaagaa tgggtctacg attctcatag
    18841 tcaagcctgg tactacttca aatccggtgg ttacatgaca gccaatgaat ggatttggga
    18901 taaggaatct tggttttacc tcaaatctga tgggaaaata gctgaaaaag aatgggtcta
    18961 cgattctcat agtcaagctt ggtactactt caaatccggt ggttacatga cagccaatga
    19021 atggatttgg gataaggaat cttggtttta cctcaagtct gatggcaaaa tggctgaaaa
    19081 agaatgggtc tacgattctc atagtcaagc ctggtactac ttcaaatctg gtggctacat
    19141 ggcgaaaaat gagacagtag atggttatca gcttggaagc gatggtaaat ggcttggagg
    19201 aaaagctaca aatgaaaatg ctgcttacta tcaagtagtg cctgtcacag ccaatgttta
    19261 tgattcagat ggtgaaaagc tttcctatat atcgcaaggt agtgtcgtat ggctagataa
    19321 ggatagaaaa agtgatgaca agcgcttggc tattactatt tctggtttgt caggctatat
    19381 gaaaacagaa gatttacaag cgctagatgc tagtaaggac tttatccctt attatgagag
    19441 tgatggccac cgtttttatc actatgtggc tcagaatgct agtatcccag tagcttctca
    19501 tctttctgat atggaagtag gcaagaaata ttattcggca gatggcctgc attttgatgg
    19561 ttttaagctt gagaatccct tccttttcaa agatttaaca gaggctacaa actacagtgc
    19621 tgaagaattg gataaggtat ttagtttgct aaacattaac aatagccttt tggagaacaa
    19681 gggcgctact tttaaggaag ccgaagaaca ttaccatatc aatgctcttt atctccttgc
    19741 ccatagtgcc ctagaaagta actggggaag aagtaaaatt gccaaagata agaataattt
    19801 ctttggcatt acagcctatg atacgacccc ttacctttct gctaagacat ttgatgatgt
    19861 ggataaggga attttaggtg caaccaagtg gattaaggaa aattatatcg ataggggaag
    19921 aactttcctt ggaaacaagg cttctggtat gaatgtggaa tatgcttcag acccttattg
    19981 gggcgaaaaa attgctagtg tgatgatgaa aatcaatgag aagctaggtg gcaaagatta
    20041 gtactataag tgaatatgat ttgagtgaat agtaagttaa aaatcctgat ttcaagtaaa
    20101 atcaggattt tttcatggat gcaatttttt tggagtctgg tgtgacgcgg agggtctttt
    20161 gtcctgtgta agtgacaaag ccgggttttc caccagttgg tttattgagt tttttgactt
    20221 caatcatatc tacctgcacc agattcgaca ggcgcccttg agagaagtag gcagctaact
    20281 ctgctgcgtc tgtcttgact gcatcagatg ggtcaagatt tcctgagatg acaacatggc
    20341 ttccaggaat gtccttagca tggaaccaaa gttcctcctt gcgggccatt ttaaaggtca
    20401 attcctcatt ttgaagattg tttcgtccga catagatgat ggttttgcca tcgcttgcta
    20461 gatattgttc tagttttttg cgtttctgga ttttctcccg ttgtcttctg cggataaaac
    20521 ctgtttgaat caattcttca cggatttcag cgatttcttc cagtccagct tggttgagga
    20581 cggtttctac actttccaga tagagaatag tggctttggt ttcttcaatc aaatcagtca
    20641 agtatttgac agcttctttg agtttctgat accgtttaaa atagcgttgg gcattctggt
    20701 tgggagtcag agccttatca agcgcaatca tgataggttg gttggtatag tagttgtcta
    20761 ggataacctg gtcttggtcg ttaggcactt ggtggaggaa ggttgtcagc aattctcctt
    20821 tttgacgaaa ttcttcagcg ttgtctgtcg ccagtaactc tttttcctgt tttttgagtt
    20881 tgtgtcggtt tttctgaagt tcattttcaa cacgacgaat cagttcactg gcctgctgtt
    20941 tgacgcggtc gcgctcagcc ttatccttat agtaggtgtc caacaaatca gaaagatttg
    21001 caaaaggctc tcccacctga tttgcaaaag gaactggact gaaggaagtc tcagtcaagc
    21061 atggcttggt ttcttgattg aaaaaatttc ggaaagcgga aagtttttca ctaaccagta
    21121 tcctttccaa ttcatttgcc gtatcgcgtc ccagaccttg aaagaggctt tgaagatttt
    21181 ttgctgttag ttcttgggtt tgtaggattt caaagagctt ttcatccttg atagtaaaag
    21241 gattgagaga ttttgtactt ggcggagcga tataggtcga tcctggaagt aaggtgcggt
    21301 agctattttg tgaaaaaccg acgtgtttga taacttcgag gattttatga ctgcttttat
    21361 cgaccagtag aatattactg tgttttccca taatttcaat aatcaaggta gcctggatat
    21421 ggtctccaat ctcgttttta ttggaaactg taatttccac aatacggtca ttttccactt
    21481 gctcaatcga ctcaatcagg gccccctgca aatactttct caaaaccatg ataaaggtag
    21541 aaggttgagc tggattttca aaagtcgttt gggtcagctg aatgcgtcca aaaactggat
    21601 gggcagaaag gagcaggcga tggctttggc gattgctgcg gatttgcaag accaactctt
    21661 gttcaaaagg ctgattgatt ttctggatgc gaccattcac taattcgctt cgcaattcct
    21721 caactatgtg gtgtaaaaaa aatccgtcaa atgacatcgt tctctccttg tgattgtatt
    21781 ccatagtatt atatcaaaaa ggtagaataa aatcatggaa atgtggtata ataaagccaa
    21841 gtaaagagaa acgagaagca catgtatatt gaaatggtag atgaaactgg tcaagtttca
    21901 aaagaaatgt tgcaacaaac ccaagaaatt ttggaatttg cagccaaaaa attaggaaaa
    21961 gaagacaagg agatggcagt cacttttgtg accaatgagc gtagtcatga acttaatctg
    22021 gagtaccgtg acaccgaccg tccgacagat gtcatcagcc ttgagtataa accagaattg
    22081 gaaattgcct ttgacgaaga ggatttgctt gaaaatccag aattggcaga gatgatgtct
    22141 gagtttgatg cctatattgg ggaattgttc atctctatcg ataaggctca tgagcaggcc
    22201 gaagaatatg gtcacagctt tgagcgtgag atgggcttct tggcagtaca cggcttttta
    22261 catattaacg gctatgatca ctatactccg gaagaagaag cggagatgtt cggtttacaa
    22321 gaagaaattt tgacagccta tggactcaca agacaataaa cgaaaatgga aaaatcgtga
    22381 cttgatatcc agtttagaat ttgccatcac agggattttt actgctatca aggaagaacg
    22441 aaatatgcga aaacatgcag tgacggctct agtggtcatc cttgcaggtt ttgtttttca
    22501 ggtgtcacga atcgaatggc tctttctcct attgagtatt ttcttggtag tagcctttga
    22561 gattatcaac tctgctattg aaaatgtggt ggatttggcc agtcactatc acttttccat
    22621 gctggctaaa aatgccaagg atatggcggc cggcgcggta ttagtggttt ctcttttcgc
    22681 agccttaaca ggcgcattga tttttctccc acgaatctgg gatttattat tttaaacagt
    22741 aagaggaaat tatgactttt aaatcaggct ttgtagccat tttaggacgt cccaatgttg
    22801 ggaagtcaac ctttttaaat cacgttatgg ggcaaaagat tgccatcatg agtgacaagg
    22861 cgcagacaac gcgcaataaa atcatgggaa tttacacgac tgataaggag caaattgtct
    22921 ttatcgacac accagggatt cacaaaccta aaacagctct cggagatttc atggttgagt
    22981 ctgcctacag tacccttcgc gaagtggaca ccgttctttt catggtgcct gctgatgaag
    23041 cgcgtggtaa gggggacgat atgattatcg agcgtctcaa ggctgccaag gttcctgtga
    23101 ttttggtggt gaataaaatc gataaggtcc atccagacca gctcttgtct cagattgatg
    23161 acttccgtaa tcaaatggac tttaaggaaa ttgttccaat ctcagccctt cagggaaata
    23221 acgtgtctcg tctagtggat attttgagtg aaaatctgga tgaaggtttc caatatttcc
    23281 cgtctgatca aatcacagac catccagaac gtttcttggt ttcagaaatg gttcgcgaga
    23341 aagtcttgca tctaactcgt gaagagattc cgcattctgt agcagtagtt gttgactcta
    23401 tgaaacgaga cgaagagaca gacaaggttc acatccgtgc aaccatcatg gtcgagcgcg
    23461 atagccaaaa agggattatc atcggtaaag gtggcgctat gcttaagaaa atcggtagca
    23521 tggcccgtcg tgatatcgaa ctcatgctag gagacaaggt cttcctagaa acctgggtca
    23581 aggtcaagaa aaactggcgc gataaaaagc tagatttggc tgactttggc tataatgaaa
    23641 gagaatacta agtagaggta ggctcatgcc tgcttcttgt ttttacagaa ggaggactta
    23701 tgcctgaatt acctgaggtt gaaaccgttt gtcgtggctt agaaaaattg attataggaa
    23761 agaagatttc gagtatagaa attcgctacc ccaagatgat taagacggat ttggaagagt
    23821 ttcaaaggga attgcctagt cagattatcg agtcaatggg acgtcgtgga aaatatttgc
    23881 ttttttatct gacagacaag gtcttgattt cccatttgcg gatggagggc aagtattttt
    23941 actatccaga ccaaggacct gaacgcaagc atgcccatgt tttctttcat tttgaagatg
    24001 gtggcacgct tgtttatgag gatgttcgca agtttggaac catggaactc ttggtgcctg
    24061 accttttaga cgcctacttt atttctaaaa aattaggtcc tgaaccaagc gaacaagact
    24121 ttgatttaca ggtctttcaa gctgcccttg ccaagtccaa aaagcctatc aaatcccatc
    24181 tcctagacca gaccttggta gctggacttg gcaatatcta tgtggatgag gttctctggc
    24241 gagctcaggt tcatccagct agaccttccc agactttgac agcagaagaa gcgactgcca
    24301 ttcatgacca gaccattgct gttttgggcc aggctgttga aaaaggtggc tccaccattc
    24361 ggacttatac caatgccttt ggggaagatg gaagcatgca ggactttcat caggtctatg
    24421 ataagactgg tcaagaatgt gtacgctgtg gtaccatcat tgagaaaatt caactaggcg
    24481 gacgaggcac ccacttttgt ccaaactgtc aaaggaggga ctgatgagaa aaatcatcgg
    24541 aatcactggg ggaattgcct ctggtaagtc aactgtgaca aattttctaa gacagcaagg
    24601 ctttcaagta gtggatgccg acgcagtcgt ccaccaacta cagaaatctg gtggtcgtct
    24661 gtttgaggct ctagtacagc actttgggca agaaatcatt cttgaaaacg gagaactcaa
    24721 tcgccctctc ctagctagtc tcatcttttc aaatcctgaa gagcaaaaat ggtctaagca
    24781 aattcaaggg gagattatcc gtgaggaact ggctactttg agagaacagt tggctcagac
    24841 agaagagatt ttcttcatgg atattcccct actttttgag caggactaca gcgattggtt
    24901 tgctgagact tggttggtct atgtggaccg agatgcccaa gtagaacgct taatgaaaag
    24961 ggaccagttg tccaaagatg aagctgagtc tcgtctggca gcccagtggc ctttagaaaa
    25021 aaagaaagat ttggccagcc aggttcttga taataatggc aatcagaacc agcttcttaa
    25081 tcaagtgcat atccttcttg agggaggtag gcaagatgac agagattaac tggaaggata
    25141 atctgcgtat tgcctggttt ggtaattttc tgacaggagc cagtatttct ttggttgtac
    25201 cttttatgcc catcttcgtg gaaaatctag gtgtagggag tcagcaagtc gctttttatg
    25261 caggcttagc aatttctgtc tctgctattt ccgcggcgct cttttctcct atttggggta
    25321 ttcttgctga caaatacggc cgaaaaccca tgatgattcg ggcaggtctt gctatgacta
    25381 tcactatggg aggcttggcc tttgtcccaa atatctattg gttaatcttt cttcgtttac
    25441 taaacggtgt atttgcaggt tttgttccta atgcaacggc actgatagcc agtcaggttc
    25501 caaaggagaa atcaggctct gccttaggta ctttgtctac aggcgtagtt gcaggtactc
    25561 taactggtcc ctttattggt ggctttatcg cagaattatt tggcattcgt acagttttct
    25621 tactggttgg tagttttcta tttttagctg ctattttgac tatttgcttt atcaaggaag
    25681 attttcaacc agtagccaag gaaaaggcta ttccaacaaa ggaattattt acctcggtta
    25741 aatatcccta tcttttgctc aatctctttt taaccagttt tgtcatacaa ttttcagctc
    25801 aatcgatcgg ccctattttg gctctttatg tacgcgactt agggcagaca gagaatcttc
    25861 tttttgtctc tggtttgatt gtgtccagta tgggcttttc cagcatgatg agtgcaggag
    25921 tcatgggcaa gctaggtgac aaggtgggca atcatcgtct cttggttgtc gcccagtttt
    25981 atttagtcat catctatctc ctctgtgcca atgcctctag cccgcttcaa ctaggacttt
    26041 atcgattcct ctttggattg ggaaccggtg ccttgattcc cggggttaat gccctactca
    26101 gcaaaatgac tcccaaagcc ggcatttcga gggtctttgc cttcaatcag gtattctttt
    26161 atctgggagg tgttgttggt cccatggcag gttctgcagt agcaggtcaa tttggctacc
    26221 atgctgtctt ttatgcgacg agcctttgtg ttgcctttag ttgtctcttt aacctgattc
    26281 aatttcgaac attattaaaa gtaaaggaaa tctagtgcga gtaaaaatta atctcaaatg
    26341 ctcctcttgt gacagtatca attacctaac cagtaaaaat tcaaaaaccc atccagacaa
    26401 gattgaggtt ttaaagtatt gtcccaagga aagaaaagta accctacatc ttgaatctaa
    26461 gtagatttta tggtaaaata atagggattt taaggagttt gatatgtata acctattatt
    26521 aaccatttta ttagtattat ctgttgtgat tgtgattgca attttcatgc aaccaaccaa
    26581 aaaccaatcc agcaatgtat ttgatgccag ttcaggtgat ttgtttgaac gcagtaaagc
    26641 tcgcggtttt gaagctgtaa tgcagcgttt gacagggatt ttagtctttt tctggctagc
    26701 cattgcctta gcattgacgg tattatcaag tagataagaa aataatgggc aggactaggt
    26761 ctttgcctct ttttattttt aaaggatgtt tgagaaggtt ttacagtaaa agaaaattaa
    26821 aaaatctaga aagaaaatat gaaagataga ataaaagaat atttacaaga caagggaaag
    26881 gtgactgtta atgatttggc tcaggctttg ggaaaagaca gttccaagga ttttcgtgag
    26941 ttgattaaaa ccttgtcctt aatggaaaga aagcaccaga ttcgctttga agaagatggc
    27001 agtctgacct tggaagccaa gaaaaaacat gagattaccc tcaaggggat ttttcatgcc
    27061 cataaaaatg gttttggttt tgtcagccta gaaggggaag aggacgacct ttttgtaggg
    27121 aaaaatgatg tcaactatgc tattgatggt gataccgtcg aggtagtgat taagaaagtc
    27181 gctgaccgca ataagggaac agcagcagaa gccaaaatta ttgatatcct agaacacagt
    27241 ttgacaacag ttgtcgggca aatcgttctg gatcaggaaa agcctaagta tgctggctat
    27301 attcgttcaa aaaatcagaa aatcagtcaa ccgatttatg ttaagaaacc agccctaaaa
    27361 ttagaaggaa cagaagttct caaagtcttt atcgataaat acccaagcaa gaaacatgat
    27421 ttctttgtcg ctagtgtact cgatgtagtg ggacactcaa cggatgtcgg aattgatgtt
    27481 cttgaggtct tggaatcaat ggacattgta tccgagtttc cagaagctgt tgttaaggaa
    27541 gcagaaagtg tgcctgatgc tccgtctcaa aaggatatgg aaggtcgtct ggatctaaga
    27601 gatgaaatta cctttaccat tgacggtgcg gatgccaagg acttggacga tgcagtgcat
    27661 atcaaggctc tgaaaaatgg caatctggag cttggggttc acattgcaga tgtttcttat
    27721 tatgtgaccg aggggtctgc ccttgacaag gaagccctta accgtgcgac ttctgtttac
    27781 gtgacagacc gagtggtgcc aatgcttcca gaacgactat caaatggcat ctgctctctc
    27841 aatccccaag ttgaccgcct gacccagtct gctattatgg agattgataa acatggtcgt
    27901 gtggtcaact ataccattat acaaacagtt atcaagacca gttttcgtat gacctatagc
    27961 gatgtcaatg atatcctagc tgtcgatgaa gaaaagagaa aagaatatca taaaattgta
    28021 tcaagtatcg aactcatggc caagcttcat gaaactttag aaaacatgcg tgtgaaacgt
    28081 ggagctctca attttgatac caatgaagcg aagattttag tggataaaca aggtaagcct
    28141 gttgatatcg ttcttcggca gcgtggcatt gccgagcgga tgattgagtc ttttatgttg
    28201 atggctaatg aaacagttgc cgaacatttc agcaagttgg atttgccttt tatctatcga
    28261 attcacgagg agcctaaggc tgaaaaggtt cagaagttta ttgattatgc ttcgagtttt
    28321 ggcttgcgta tttatgggac tgccagtgag attagtcagg aggcacttca agacatcatg
    28381 cgtgctgttg agggagaacc ttatgcagat gtattgtcca tgatgcttct tcgctctatg
    28441 cagcaggctc gttattcgga gcacaatcac ggccactatg gactagctgc tgactattat
    28501 actcacttta ccagtccaat tcgtcgttat ccagaccttc ttgttcaccg tatgattcgg
    28561 gattacggcc gttctaagga aatagcagag cattttgaac aagtgattcc agagattgcg
    28621 acccagtctt ccaaccgtga acgtcgtgcc atagaagctg agcgtgaagt cgaagccatg
    28681 aaaaaggctg agtatatgga agaatacgtg ggtgaagagt atgatgcagt tgtatcaagt
    28741 attgtcaaat tcggtctctt tgtcgaattg ccaaacacag ttgaaggctt gattcacatc
    28801 actaatctac ctgaatttta tcatttcaat gagcgtgatt tgactcttcg tggagaaaaa
    28861 tcaggtatca ctttccgagt gggtcagcag atccgtatcc gtgttgaaag agcggataaa
    28921 atgactggag agattgattt ttcattcgta cctagtgagt ttgatgtgat tgaaaaaggc
    28981 ttgaaacagt ctagtcatag tggcagaggg cgtggttcaa atcgtcgttc ggataagaag
    29041 gaagacaaga gaaaatcagg acgctcaaat gataagcgta agcattcaca aaaagacaag
    29101 aagaaaaaag gaaagaaacc tttttacaag gaagtagcta agaaaggagc caagcatggc
    29161 aaagggcgag ggaaaggtcg tcgcacaaaa taaaaaggca cgccacgact atacaatcgt
    29221 agatacgcta gaggcaggga tggtcctgac tggaactgaa atcaagagtg tacgagctgc
    29281 tcgaattaat ctcaaggatg gctttgctca agtgaaaaat ggagaagttt ggctgagcaa
    29341 tgttcatatc gcgccttacg aagagggcaa tatctggaac caggaaccag aacgtcgtcg
    29401 taaactcctg ctccataaaa agcaaattca aaaattggaa caagagacca aagggacagg
    29461 aatgacctta gttcccctta aggtctatat aaaagatggc tacgctaagc ttcttttagg
    29521 acttgccaaa gggaagcatg actatgacaa acgggagtct atcaaacgtc gtgagcaaaa
    29581 tcgagatatc gcgcgtgtga tgaaagctgt taatcagcga taaaaagagg aattgaaaat
    29641 ggaaaaatta gttgcctata aacgcatgcc tttgtggaat aaacaaacaa tgcctgaagc
    29701 tgttcagcaa aagcacaata caaaagttgg gacttggggg aaaattactg tcttgaaggg
    29761 agctctcaag tttattgaat tgacagaaga aggggaagtt ctagctgaac acctctttga
    29821 agcaggggca gacaatccaa tggcccaacc tcaagcctgg caccgagtgg aagctgccac
    29881 agatgatgtg gaatggtact tggaatttta ttgtaaacct gaggattatt ttgctaaaaa
    29941 atacaatacc aatcctgttc attcagaggt cctagaggcc atgcagacag tgaaacaagg
    30001 gaaagctttg gatttgggtt gtggtcaggg acgtaattct ctttttctag cccagcaaga
    30061 ttttgatgtg acggctgtag atcaaaatgg actagctctt gaaatcttgc aaagcattgt
    30121 ggagcaggaa gatttggaca tgcctgttgg cctttacgat atcaattcag ctagcattga
    30181 acaagaatat gattttatcg tttcaacagt tgttctcatg tttctacaag cggaccgcat
    30241 tccagctatt attcaaaata tgcaggagaa aaccagtgtt ggtggttaca accttatcgt
    30301 ttgtgccatg gacacggagg attatccttg ctcggttaac ttcccattca cctttaaaga
    30361 aggagaactg gcagactatt acaaggattg ggaattggtt aagtacaatg aaaatccagg
    30421 ccatttgcat cgtcgcgatg agaatggcaa tcgtattcaa ctacgctttg cgaccttact
    30481 agctaagaaa atcaagtaaa cacacatgaa gattaggaat tttcctgatc ttttttcttt
    30541 tttacgaatg atatagaaaa ggagggaatt catgtttgtt gcgagagatg ctaggggaga
    30601 attggtaaat gtgttagagg ataaacttga gaagcaagca tacacctgcc cagcttgtgg
    30661 aggccagatc catttgcgtc aaggaccaag tgtacggacg cattttgccc ataaatcctt
    30721 aaaagactgt gattttttct ttgaaaatga aagtccagaa cacatagcca ataaggaatc
    30781 cctctatcac tggttgaaaa aagagacaaa ggttcaatta gagtacccgc tttcagaact
    30841 taaacagatt gcggatgtat ttgtaaatgg caatctagct ctagaagttc agtgtagtcc
    30901 cttgcctcag aaagtcctta aagagcgaag tgagggctat cgtagtcagg gttaccaagt
    30961 actgtggttg ctgggtaaaa aactgtggct caaggagcgt ttgacttgtc tacagcaagg
    31021 ttttctttat ttcagtcaaa acatgggctt ttatgtttgg gaattagaca aggaaaaaca
    31081 agttttaaga ctcaaatacc tgatttacca ggatctccgc ggtaaactcc attatcaaat
    31141 caaggaattt tcctatggtc aaggtagttt attggaaata ttgcgtcttc cctataagag
    31201 acaaaaaata tctcatttta cagtttctga ggacaaggac atctgtcgct atatccggca
    31261 acaactttat tatcaaaatc tcttttggat gaaagaacaa gcagaagcct atcaaaaggg
    31321 agaaaatatc ctgacttatg gactgaaaga atggtatcca caaattcgac caatagtggg
    31381 caaatttttc cagattaaac aagacttgac tagctattat cagcactttt atacctatta
    31441 ccaaaaaaat cctcaaaatg attggcaaaa gctttatcca ccagcctttt atcagcaata
    31501 tttcttgaaa aatatggtag aatagaaagg atggaggaat ctaatggtat tacaaagaaa
    31561 tgaaataaat gaaaaagata catgggatct atcaacgatc tacccaactg accaggcttg
    31621 ggaagaagcc ttaaaagatt taacagaaca attggagaca gtagcccagt atgaaggcca
    31681 tctcttggat agtgcggata acctactaga aatcactgaa ttttctcttg aaatggaacg
    31741 ccagatggag aagctttacg tttatgctca tatgaagaat gaccaggata cacgtgaagc
    31801 taagtatcaa gagtactatg ccaaggccat gacactctac agccagttag accaagcctt
    31861 ttcattctat gagcctgaat ttatggagat tagcgaaaag cagtatgctg actttttaga
    31921 agctcaacca aagctgcagg tttatcaaca ctattttgac aagctcttgc aaggcaagga
    31981 tcacgttctc tcacaacgtg aagaagaatt attggctgga gctggagaaa tctttggttc
    32041 agcaagtgaa accttcgcta tcttggacaa tgcggatatt gtgttccctt atgtcctaga
    32101 cgatgatggt aaagaagttc agctatctca tgggacttac acacgtttga tggagtctaa
    32161 aaaacgtgag gttcgccgtg gtgcctatca agctctttat gcgacttacg aacaattcca
    32221 acacacctat gccaaaacct tgcaaaccaa tgttaaggtg caaaattacc gtgctaaagt
    32281 tcgtaactac aagagtgctc gtcatgcagc cctcgcagcg aattttgttc cagaaagtgt
    32341 ttatgacaat ttggtagcag cagttcgcaa gcatttgcca ctcttacatc gctatcttga
    32401 gcttcgttca aaaatcttgg ggatttcaga tctcaagatg tacgatgttt acacaccgct
    32461 ttcatctgtt gaatacagtt ttacctacca agaagccttg aaaaaagcag aagatgcttt
    32521 ggcagtcttg ggtgaggatt acttgagccg tgttaaacgt gccttcagcg agcgttggat
    32581 tgatgtttac gaaaatcaag gcaagcgttc aggtgcctac tctggtggtt cttacgatac
    32641 caatgccttt atgcttctca actggcagga caatctggac aatctcttta ctcttgttca
    32701 tgaaacaggt cacagtatgc attcaagcta tactcgtgaa actcagcctt atgtttacgg
    32761 agattactct atctttttgg ctgagattgc ctcaactacc aatgaaaata tcttgacgga
    32821 gaaattattg gaagaagtgg aagacgacgc aacacgtttt gctattctca ataacttcct
    32881 agatggtttc cgtggaacag ttttccgcca aactcaattt gctgagtttg aacacgccat
    32941 tcaccaagca gatcaaaatg gggaggtctt gacaagcgat ttcctaaata aactctacgc
    33001 atacttgaac caagagtatt atggtttgag taaggaagac aatcctgaaa tccaatacga
    33061 gtgggctcgc attccacact tctactataa ctactatgta tatcaatatt caactggctt
    33121 tgcggccgcc tcagccttgg ctgaaaaaat tgtccatggt agtcaagaag accgtgaccg
    33181 ctatatcgac tacctcaagg caggtaagtc ggactatcca cttaatgtca tgagaaaagc
    33241 tggtgttgat atggagaagg aagactacct caacgatgcc tttgcagtct ttgaacgccg
    33301 tttaaatgag tttgaagccc ttgttgaaaa attaggattg gcataaaatg gttgaatcgt
    33361 atagtaagaa tgctaaccat aacatgcgtc gtcctgtcgt caaagaagaa attgtagact
    33421 tgatgcgtca gcgtcaaaag caagtcacag gtttcttgaa agaattggaa gactttgccc
    33481 gcaaggaaaa tattcctatt attccccatg aaacggttgc ttatttccgt tttcttatgg
    33541 aaaccatgca gcctaaaaat attctggaaa ttgggacggc tatcggtttt tcagctctct
    33601 tgatggctga acatgcgcca aatgctaaga ttacaactat tgatcgtaat ccagaaatga
    33661 ttggttttgc caaggaaaat tttgcccagt ttgacagtcg caagcaaatc actctcctag
    33721 agggagatgc ggtggatgtc ttatctacac tgacagagtc ttatgatttc gtctttatgg
    33781 attctgccaa gtctaaatac atcgtctttc tgccagaaat cctcaaacat ttggaagttg
    33841 gtggtgtggt tgtcttggat gatatttttc aaggtggtga tgttgccaag gatattatgg
    33901 aagtccgtcg tggtcagcga accatttatc gaggccttca aaaattattt gatgcaacct
    33961 tagacaatcc agaactcacc gcaacattag tgcctttagg agatggtatt ctcatgcttc
    34021 gtaaaaatgt agcagatgtt caactgtctg aaagcgaatg attttcagaa aaatttaaga
    34081 aaaaatagta aaatagatag agtaacactt atctcaaagg agtagacatg aagaaaaaat
    34141 tattggcagg tgccatcaca ctattatcag tagcaacttt agcagcttgt tcgaaagggt
    34201 cagaaggggc agaccttatc agtatgaaag gggatgtcat cacagaacat caattttatg
    34261 agcaagtgaa aaacaaccct tcagcccaac aagtcttgtt aaatatgacc atccaaaaag
    34321 tttttgaaaa acaatatggc tcagagcttg atgataaaga ggttgatgat actattgccg
    34381 aagaaaaaaa acaatatggc gaaaactacc aacgtgtctt gtcacaagca ggtatgactc
    34441 ttgaaacacg taaagctcaa attcgtacaa gtaaattagt tgagttggca gttaagaagg
    34501 tagcagaagc tgaattgaca gatgaagcct ataagaaagc ctttgatgag tacactccag
    34561 atgtaacggc tcaaatcatc cgtcttaata atgaagataa ggccaaagaa gttctcgaaa
    34621 aagccaaggc agaaggtgct gattttgctc aattagccaa agataattca actgatgaaa
    34681 aaacaaaaga aaatggtgga gaaattacct ttgattctgc ttcaacagaa gtacctgagc
    34741 aagtcaaaaa agccgctttc gctttagatg tggatggtgt ttctgatgtg attacagcaa
    34801 ctggcacaca agcctacagt agccaatatt acattgtaaa actcactaag aaaacagaaa
    34861 aatcatctaa tattgatgac tacaaagaaa aattaaaaac tgttatcttg actcaaaaac
    34921 aaaatgattc aacatttgtt caaagcatta tcggaaaaga attgcaagca gctaatatta
    34981 aggttaagga ccaagccttc caaaatatct ttacccaata tatcggtggt ggagattcaa
    35041 gctcaagcag tagtacatca aacgaatagt ccaaatcaat gagtcaggga aaaaactcga
    35101 cttcaggaaa aaatgaagca aacattccca caataaaacg catagtacaa ggtttgtact
    35161 gccccccaaa aaagttagac aattaattta tccgaaggat ttagttctgt attgcacaga
    35221 gctaagtcct tttagtttta tcttaattct cttattgttg taataatcaa tatagtctat
    35281 aatggcttgt tccaattgat taagtgattt aaatgtttgc tcatagccat aaaacatttc
    35341 ggattttaaa atgccaaaga aagattccat cctaccgttg tcttggctgt ttcctttgcg
    35401 tgacatagat gcttgaattc ccttactctc taggaagcga tgataagaat cgtgttggta
    35461 ttgccagcct tggtcactat ggagaatcgt attctcgtag tgcttctctt tgaatgcctg
    35521 ttccaacatt aacgatcaat caatttaatc atgtacctaa gattagaatt gtttatccca
    35581 aatttatttg aaagcttctc taagcttaag ctatatcctt gttttctaag ttcatagatc
    35641 tgaactttat catcataagt taatttcata ataaaaacac cccaaaagtt agattttttc
    35701 tgtctaactt ttggggtgta gttcatgtac acctgatatg atgcgtttta taattttaaa
    35761 gactttttga ccagcctcat ttttttaact tgatactcag tgaaaagcaa agattaaact
    35821 aggaagctag ctgtaggctg ctcaaagaac agctttgagg ttgcagataa aacttgtgag
    35881 gtaaccaaca tatataatgt gaagctgacg tggtttgaat agattttaga agggtatgag
    35941 tctggaagtt ttaatggata atgcaagatt ccatagaatg ggtaagctag agttcttatg
    36001 tgaagagttt gggcataaac ttttaccttt tcctccctac tcatcttagt atagaaaagt
    36061 gaatctaaaa tagtacataa ctgcttctaa aacattctta taaattgatt taaattctca
    36121 aatcatatta ttcagttctt atttcatttt gctctacaat cctgttgaga agacacgtgt
    36181 tcatatcaaa aaggtattgg caagttgcaa taccttttta caaggttctt ttgtcttatt
    36241 tttgtttcaa ctgactatat ctcctatggt tctagttcag aaggctaggc tataattatg
    36301 attgataaga agtatcattc caagtattga gagtgaatgt ttcaaaatca tgggtttcta
    36361 taatggtcag gctggcattt gctagaccgc catctttacg aagaagtggt tctttatagc
    36421 ctaggagagt acgaagactg gcagtaagat tggcgccgtg tccgacaatt agaatacgct
    36481 cagctggact atcttttaat gatttgataa attggatggt ccgctgagtt gtactataga
    36541 gggattcggc tccgaacatt cgagtgtcaa attgagcaag atttgaacga aaagcctgga
    36601 tttgttgcgg gtaaatagct tccagggttg caattttcaa accttctaac ttcccaagtt
    36661 gccattcacg gagattagga acgatttcta aagaacaggg ggtatggagt tgactttgga
    36721 taatctcagc agatttgacc gctcgaggta aatcacttga ataaatctga tcaaaaggaa
    36781 tttccttgag atactggcca agtcgtttta gggtttcaat ggatccagga agaagaggag
    36841 aatcaccgct agcaccttga aaacgacctt cttggttcca gagggtacga ccgtggcgga
    36901 caaagtagag tttcattact tgatgtcctc caaaatatct acaaagtctg cctttacaaa
    36961 gctagccaag tcttgtggcg cgacgataat gctgtgtccg acttcgcctg cagagacaat
    37021 cattcgatcc aaatctagag caattttatc gataaaaatg ggataattgt gtttctgacg
    37081 aattccgaca ggattattgg ctccatgaat gtaaccagtt gttttttcta agtccttttg
    37141 tggaatcatg ctcatttttt tattgccaga aattttagct agtttctttt cagacaagtg
    37201 ctgagtgata gggacaattc cgataatcgg tccggtcttg tctcccaaaa gcgccaaggt
    37261 tttgaaaatc tgatctcgtt cataaccttg aggaagctct ccttctaggg cattgatttg
    37321 aatcccctga tgagggatag ctgctttaga taggatttgt tccaccaatg tttttttgat
    37381 tttaactttt tttgccatta tttatattta tcctccaatt gactcatcca aataccaagc
    37441 cagattccca gcgcaaagaa gaaggcgatg atgacataac cgacaagtga aagtcctgtg
    37501 tattggatac tttcagcgtt tcctgcattt ggaattaaga tcaaaagggt acttgatagg
    37561 acgataccga tgatgaaatg atagacgcgt gagtggtagt tgtttaaggc ataatccatc
    37621 aattttgaaa aaacgatgag agttgcacct gcaccaattc caatcggaaa gaaggttccc
    37681 aagagatcaa aagttttaaa accagtcaac ataggagcat agagtcccaa aatcaaaagt
    37741 aaattcgatg ggctgaggcc aggaaccaag acgccaaggg ccaatagtgc gcctgctagg
    37801 acgaagttaa gaaagctggc gcttaaggtt ccaacgacaa aatttaaggc atagagtcct
    37861 aatccagaaa tgataaaggt tgtccataac caagctaaat caatcttgtc tcggtcagat
    37921 tctcgagttg attctttgag gaggctagga actgtaccga taatagctcc cgcaaagctc
    37981 cataatacaa agacctgata attttcaagc aggtattcaa tcgggtagga aaataagccg
    38041 attcccagaa gcataccgat ggcaactgga ataaagtaca aaacattttc tttaaagtct
    38101 ttaaagggat gggccagaaa gccaatcatt cgttcataga ttcctaagat tgctgctaga
    38161 acccctccgg aaattcccgg taggataaat ccaagagcaa ttacaatccc tttaataacg
    38221 cgtgctaacc atgagagcat atatttcctc caactatttc tatttcaaaa aatccttact
    38281 atattgtatc acaatcagac acaaaaagaa aaagcaaatg ataaacaaat gcttttaagg
    38341 ttttaaaaaa agttttcgaa aggtttcttc tttatttttt aagggagaga taacgttgat
    38401 atctaaatcg tggtcaaagc cggcaatttt tcctttagat gtgtattggt gaatatcata
    38461 atctaaatca gttttaggac tgctttccaa aaatcctgag tctgagccgt agaacggaat
    38521 ccaaacagag gtaaacttgc ctgtatcaat actgtgttct tccatgaagt agacaccaac
    38581 gtagatgccg atgtttttag cacccagtga tgctagtttt gctcgaaagt tttcgacacc
    38641 ttcgttcata ttagacatgg ttttgtcttc cacgtcaagc caatagtaac tagggctgta
    38701 aggagaggca gcattgtaga aaacttcggc agccttttcc atttcttgga cactttttcc
    38761 agctacataa gcgtagacag caactgggac attccgcttt tgaagttcag tgatatgact
    38821 cttataggcc ttgtctattc cattgataaa tgaagcatca ttttcttttg tcgtttgagc
    38881 accactgtga acacgaacaa tagcacctga aatattttgt gagagggcat cgtagttgat
    38941 ttcctcagga cgctgccagc cagagaggtc aataatcggt ttgtctaagt gtttcaaagc
    39001 ctgtgcttca atctgtgcta tattggattt tgttttaaac gattggctgt cattaagtgg
    39061 gcgattgatg attaaaatga acatcataat cccaaaaaaa ctaaataaaa taagtggatg
    39121 aatttgtttt ctcatatctt ataattctac cctaaaaatc aaaaaaaatc aaaaaaatgg
    39181 gttaaggaag agactttaga gcattttttc attcaagagt gcggaatgat ttgaaatatg
    39241 gtataataaa agggaatttc tacagaaaag ggaagattat gtcaaatttt gccattattt
    39301 tagcagcggg taaagggact cgcatgaaat ctgatttgcc aaaagttttg cacaaggttg
    39361 cgggtatttc tatgttggaa catgttttcc gtagtgtggg agctatccaa cctgaaaaga
    39421 cagtaacagt tgtaggacac aaggcagaat tggttgagga ggtcttggct ggacagacag
    39481 aatttgtgac tcaatctgaa cagttgggaa ctggtcatgc agttatgatg acagagccta
    39541 tcttagaagg tttgtcagga cacaccttgg tcattgcagg agatactcct ttaatcactg
    39601 gtgaaagctt gaaaaacttg attgatttcc atatcaatca taaaaatgtg gccactatct
    39661 tgactgctga aacggataat ccttttggtt atggacgaat tgttcgtaat gacaatgctg
    39721 aggttcttcg tattgttgag cagaaggatg ctacagattt tgaaaagcaa atcaaggaaa
    39781 tcaacactgg aacatacgtc tttgacaacg agcgtttgtt tgaggctttg aaaaatatca
    39841 ataccaataa cgctcaaggc gaatactata ttacagacgt cattggtatt ttccgtgaaa
    39901 ctggtgaaaa agttggcgct tatactttga aagattttga tgaaagtctt ggggtaaatg
    39961 accgtgtggc gcttgcgaca gctgagtcag ttatgcgtcg tcgcatcaat cataaacaca
    40021 tggtcaacgg tgttagcttt gtcaatccag aagcaactta tatcgatatt gatgttgaga
    40081 ttgcttcgga agttcaaatc gaagccaatg ttatcttgaa agggcaaacg aaaattggtg
    40141 ctgagactgt tttgacaaac ggtacttatg tagtggacag cactatcgga gcaggagcgg
    40201 tcattaccaa ttctatgatt gaggaaagta gtgttgcaga cggtgtgata gtcggtcctt
    40261 atgctcacat tcgtccaaat tcaagtctgg gtgcccaagt tcatattggt aactttgttg
    40321 aggtgaaagg atcttcaatc ggtgagaata ccaaggctgg tcatttgact tatatcggaa
    40381 actgtgaagt gggaagcaac gttaatttcg gtgctggaac tattacagtc aactatgacg
    40441 gcaaaaacaa atacaagaca gtcattggaa acaatgtctt tgttggttca aattcaacca
    40501 ttattgcacc agtagaactt ggtgacaatt ccctcgttgg tgctggttca actattacta
    40561 aagacgtgcc agcagatgct attgctattg gtcgcggtcg tcagatcaat aaagacgaat
    40621 atgcaacacg tcttcctcat catcctaaga accagtagga gcctatcatg gagtttgaag
    40681 aaaaaacgct tagccgaaaa gaaatctatc aaggaccaat atttaaactg gtccaagatc
    40741 aggttgaatt accagaaggc aagggaactg cccaacggga tttgattttc cacaatgggg
    40801 ctgtctgtgt tttagcagta acggatgaac aaaaacttat cttggtcaag cagtaccgca
    40861 aagctatcga ggctgtctct tacgaaattc cagccggaaa attggaagta ggagaaaaca
    40921 cagcccctgt ggcagctgcc cttcgtgaat tagaggaaga aacagcctat acagggaaat
    40981 tagaactctt gtacgatttt tattcagcta ttggcttttg taatgagaag ttaaaactat
    41041 atttagcaag cgatttgaca aaagtggaaa atccgcgtcc gcaggatgag gatgaaacct
    41101 tggaagtcct tgaagtgagc ttagaagaag cgaaagaatt aatccaatca ggtcatattt
    41161 gtgatgccaa gacaattatg gctgttcagt attgggagtt gcagaaaaaa tagaggaggt
    41221 cagtatgggt aaatctttat taacggatga aatgattgaa agagctaata gaggcgaaaa
    41281 aatttcaggt cctcctttgc tagatgataa tgaggaaact aagattttac caacctcttc
    41341 ttcccgtttt ggttatgcca atcctaagga tcatggtttt agccaggaaa ccttgaagat
    41401 tcaggtcgaa ccatctattc ataaaagccg tcgtattgaa aataccaaga gaaatgtctt
    41461 caattctaag ttgaataaaa tcttatttgc ggtcatcttt ctcttgattt tgcttgtttt
    41521 agcaatgaaa cttttgtaat agaaaaggaa ttgaaatgaa aataggaatt attgctgcta
    41581 tgccagaaga actggcttat ctggtccagc atttagataa tgcccaggag caagttgttt
    41641 tggggaatac ctatcataca ggaaccattg cttctcatga agtcgttctt gtagaaagtg
    41701 gaattggtaa ggtcatgtct gctatgagtg tggcgatttt ggctgatcat ttccaggtgg
    41761 atgcccttat taatacgggt tcagctgggg cagtagcaga aggtatcgct gttggggatg
    41821 tcgtgattgc tgacaaatta gcctatcatg acgtggatgt cacagctttt ggctatgctt
    41881 atggacaaat ggcgcaacaa ccgctttatt tcgaatcaga caaaaccttt gttgctcaaa
    41941 tccaagagag tttatctcaa ttggaccaaa actggcatct tggtttgatt gctacaggag
    42001 atagttttgt tgcaggaaat gacaagatag aagcgattaa gtcccatttc ccagaagttt
    42061 tagccgtgga gatggagggg gcagctattg ctcaagcagc gcatgccctc aatctcccag
    42121 tcttagtcat ccgagctatg agtgacaatg ccaaccatga agcaaacatc ttttttgatg
    42181 agtttattat cgaagctgga cgtcgctctg cccaagtctt gttgaccttt ttgaaggctt
    42241 tagattaagc ggaaatttga cagtttttct agcttatgat aagatttaag taaagaaaag
    42301 ctagaaaacg tttcagagga tattatgagt attgaaatga ccgtcagtga gattgcagag
    42361 gtcttaggat tatctcgcca agcaatcaat aaccgtgtca aagaattacc agaagaagac
    42421 acagataaaa atgacaaagg ggtaacagtt gttaccagaa gtggcttgat taagctagaa
    42481 gaaatctata aaaaaacgat ttttgaagat gagcctgtca gtgaagatgt caaacaacgt
    42541 gaactgatgg agattctagt ggatgagaag aatgcagaaa ttcttcgtct ttatgaacaa
    42601 ttaaaagcca aggatcgtca gttatcagaa aaagacgagc agatgcgtat caaagaccgt
    42661 cagattgctg agaaagacaa acaattagat cagcaacaac aattgaccct tcaagccatg
    42721 aaggatcaag aaaatcttaa gctagagctg gaccaagcaa aagaagaagt ccaatccact
    42781 aagaaaggct tttttgctcg tttatttgga ggataatcaa ggagctgttt aggttaaatg
    42841 ctaacctgat ggcttctttt gtttgtgaat agtatagttg attagaacag agacagtaga
    42901 ggagaggtac agaatggaac gattagaaga ttacattgct tttgacttag aatttaatca
    42961 gcacgaagga caaacacact tgattcaagt atcggcagtg cgttttagag atggtcggga
    43021 aatcgatgcc tatgattcct atgttcatac cagcgtccct ctaaaaagct ttatcaatgg
    43081 tttgaccggg attacagctg agaccttaaa agatgctcct ccagttgaga aagtcttaga
    43141 agaattccaa gagtttgtgg gttctttgct gatgataggc tacaatgctg ctaaaagtga
    43201 tttacctatt ttagcagagc atggtttaga ctatagccaa cagtacaagg tggatttgta
    43261 tgatgaagct tttgaacgtc ggagttcaga tttacatggt attgccaatc tcaaattgca
    43321 aactgttgcc tcatttcttg gatttcaagg tcagtctcat aatagtttag aggatgctcg
    43381 gatgacggcg cgtgtttacg aatccttctt agagtcggat caagctaggg aattgttaga
    43441 gacagaaagt agcttatcaa ataatccttt tggtggcttg gacttgtctc aattatttga
    43501 ctaggagtgc ctatgaaacc tgaaaaacct aaaaaattgg gtcttaaaca gatttacctc
    43561 aatcttgata aaatcctctt tctatttttc ttgattttta tgatagtgga gtatttgtgg
    43621 gtaccattca attcatggtt ggctagcttc ttattagatc agacaggtta tctttttatt
    43681 tcctataaca atgtcttagc catccttact cattctccat tgattagtct agctttttta
    43741 gccttgattg taattaactt tctggttgct tattttcaaa taggactcct ctttattggt
    43801 gctcgtcatc tcctctataa tgaaaagcga aggctaattg agtatatccg caaggttttc
    43861 catgaaagtt ttgaatttat gaaaaagcta actggcgcta aagccttgtt tatctttttc
    43921 tatatagcta tgctgtttcc ttttatacgg aaaatgctga agatttatta tcttaataaa
    43981 atcgtcattc cagagtttat ccaagcttat ttagaagata aatattggat gtggtggctg
    44041 agcatcctac ttctatctct tatctttctc tatgtctctg tcagattgat gtttgccctt
    44101 ccgaagattg tatatgatca gctgactgtt cgagaagcag tgatgtttag cttggagaaa
    44161 accaagaaga gagttacttt ttatgcttgg aatttatttt acatcttgct caaagcacat
    44221 ttattatttt atcttccttt gattccttta ttattggctc aaactttggt agatgatatc
    44281 actcagagag agtctctgat tcttggtatt ttgaattttg ttgtgattaa aaatctctat
    44341 tatatggctc tgacttattt tttggttaaa tttgtttcat ttttgacagg aaaggagctg
    44401 gatatgcttc ctaggagaga aaaagatcac atcgtgcgat ggggtgtcat gacttgtgcc
    44461 agtcttttct ttgccttaga aggttatatt tatctggagg ctcccatggt tcatctacct
    44521 caacttattt ctcaccgagg ggtttccaat gcaaatggga ttcaaaatac agtagagtcc
    44581 ttggaaacta cagcacaact caaaccagac ttggtggaga cggacgtgca ggaaacaaaa
    44641 gatgggcagt ttgtcatgat gcatgatgct aacttgaaaa atctagcagg tatcaataaa
    44701 agtcctcaag acttaaactt ggaggagctt aaagggattg atatttttga aaatggctac
    44761 cagactaaaa tttcaagctt tgaagattat ctcagtcgag ccaacgaact tggtcaaaaa
    44821 ttactaattg aaattaaaac cagtaaaaaa gatagtccag acatgatgaa ccgcttttta
    44881 gcccgttatg ctgcaaagct caagatttat ggacatcaaa tccagtcttt agactaccat
    44941 gttgtcgaaa aagtaagaca gtatgatgcc gaactgccag tttatttcat catgccctac
    45001 aattctgtct ttcctaaaac aagagcgaca ggatatacta tggagtactc aaccttggat
    45061 gaatattttg taagcaaact atggacaacg gatcagaaac tttatgtctg gactgtcaat
    45121 gattcagaag ctatcagcaa atctcttcac ttaggagtag atggagtgat tacagatgat
    45181 ttggaaaaag ttcagcggga aatagaagta gcccaagaag atccagaata tacggatttg
    45241 ctcttgaaaa aagcattgga attctttgaa ttttagtaat aaaaaagagg aacggttgtt
    45301 cctcccatga taggtaagta ttcttggctc tttgtcaact gtagtgggtt gaaaaaaagc
    45361 taagcttgag aaaggacaaa tttcgtcctt tcttttttga tattcagggc gataaaaatc
    45421 cgttttttga agttttca
//
LOCUS       DBEROU010000011        43510 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.011, whole genome shotgun sequence.
ACCESSION   DBEROU010000011 DBEROU010000000
VERSION     DBEROU010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 43510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.011"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            584..958
                     /gene="dhaM"
                     /locus_tag="ACL8CL_000676"
     CDS             584..958
                     /gene="dhaM"
                     /locus_tag="ACL8CL_000676"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002960428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HGS1329967.1"
                     /translation="MESIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGGIG
                     TSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNAAA
                     LLQAGAELSVIQTQLAELEINK"
     gene            1416..2129
                     /locus_tag="ACL8CL_000677"
     CDS             1416..2129
                     /locus_tag="ACL8CL_000677"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HGS1329968.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFSHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIERDYDLYAATNGITAIQTGRLAQSGLVPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            2163..2765
                     /locus_tag="ACL8CL_000678"
     CDS             2163..2765
                     /locus_tag="ACL8CL_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329969.1"
                     /translation="MTIEEYNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDNLRVIGNIFPNYLTNRKYSLNMNRNGCMGDFPHDFF
                     DIYLDHVAKYAYEQKVNNIKEYYPLKRAILHQENALYFRLFSNFDDFLEKNYLKTIWQ
                     VSKETPFSEMDFNMFKNISEKIIFEIGSKMLNDLKSNYKK"
     gene            2903..3556
                     /locus_tag="ACL8CL_000679"
     CDS             2903..3556
                     /locus_tag="ACL8CL_000679"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HGS1329970.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGTIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANAFLKETGQEPIDWLR"
     gene            3566..4030
                     /locus_tag="ACL8CL_000680"
     CDS             3566..4030
                     /locus_tag="ACL8CL_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HGS1329971.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPDLDWYTYVFKVTEFEGDLIDCNEGT
                     LEWVPYDEVLSKPTWEGDHTFVEWLLGDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            4043..5311
                     /locus_tag="ACL8CL_000681"
     CDS             4043..5311
                     /locus_tag="ACL8CL_000681"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HGS1329972.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGLVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFEAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            5325..5585
                     /locus_tag="ACL8CL_000682"
     CDS             5325..5585
                     /locus_tag="ACL8CL_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329973.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            5596..6957
                     /gene="pdrM"
                     /locus_tag="ACL8CL_000683"
     CDS             5596..6957
                     /gene="pdrM"
                     /locus_tag="ACL8CL_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HGS1329974.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTVRFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            7111..8079
                     /locus_tag="ACL8CL_000684"
     CDS             7111..8079
                     /locus_tag="ACL8CL_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HGS1329975.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            8095..9087
                     /locus_tag="ACL8CL_000685"
     CDS             8095..9087
                     /locus_tag="ACL8CL_000685"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HGS1329976.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDNNSVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            9305..10348
                     /locus_tag="ACL8CL_000686"
     CDS             9305..10348
                     /locus_tag="ACL8CL_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HGS1329977.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     RVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPCINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGVQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            10394..12097
                     /gene="lpdA"
                     /locus_tag="ACL8CL_000687"
     CDS             10394..12097
                     /gene="lpdA"
                     /locus_tag="ACL8CL_000687"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003001382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HGS1329978.1"
                     /translation="MALEVIMPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKV
                     SMELEAEEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTS
                     NDDGKSDDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTY
                     LHNAEIIENISHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTV
                     HKGIGTITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNE
                     VPESLVIIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDAEVSKNLRLILERKGM
                     TILTGTKLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCI
                     KVNEYMETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIY
                     TLPEVAAVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGV
                     HIIGPAAAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKK
                     K"
     gene            12161..13150
                     /locus_tag="ACL8CL_000688"
     CDS             12161..13150
                     /locus_tag="ACL8CL_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HGS1329979.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAEINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFVGISREEIAEAVVG"
     gene            complement(13223..14293)
                     /gene="xerS"
                     /locus_tag="ACL8CL_000689"
     CDS             complement(13223..14293)
                     /gene="xerS"
                     /locus_tag="ACL8CL_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HGS1329980.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETAGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVSDEQKNALDSL"
     gene            complement(14877..16427)
                     /locus_tag="ACL8CL_000690"
     CDS             complement(14877..16427)
                     /locus_tag="ACL8CL_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HGS1329981.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHLIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(16443..17222)
                     /locus_tag="ACL8CL_000691"
     CDS             complement(16443..17222)
                     /locus_tag="ACL8CL_000691"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HGS1329982.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFSGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(17209..18060)
                     /gene="ylqF"
                     /locus_tag="ACL8CL_000692"
     CDS             complement(17209..18060)
                     /gene="ylqF"
                     /locus_tag="ACL8CL_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HGS1329983.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEAPVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
     gene            18142..18327
                     /locus_tag="ACL8CL_000693"
     CDS             18142..18327
                     /locus_tag="ACL8CL_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329984.1"
                     /translation="MMSKQFLFYDIEIYFILYHAFFILSDDSLLPYLLPYLLPYIEKS
                     HQMMTFQREQKTVPVIL"
     gene            complement(18291..18467)
                     /locus_tag="ACL8CL_000694"
     CDS             complement(18291..18467)
                     /locus_tag="ACL8CL_000694"
                     /inference="COORDINATES: protein motif:HMM:NF012948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS1329985.1"
                     /translation="MQAGIPTDNEVSYDLIYQQETLPATGSSTSELTALGLLAVGSLV
                     LLVHNMTGTVFCSL"
     gene            complement(18519..24533)
                     /gene="iga"
                     /locus_tag="ACL8CL_000695"
     CDS             complement(18519..24533)
                     /gene="iga"
                     /locus_tag="ACL8CL_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin A1 protease"
                     /protein_id="HGS1329986.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAAMGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSESEVSNQKSSVATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPSPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGEPEVQPTLPEAVVTDKG
                     ETEVQPESPDTVVSDKGEPEQVAPLPEYKGNIEQVKPETPVEKTKEQGPEKTEEVPVK
                     PTEETPVNPNEGTTEGTSIQEAENPVQPAEESTTNSEKVSPDTSSKNTGEVSSNPSDS
                     TTSVGESNKPEHNDSKNENSEKTVEEVPVNPNEGTVEGTSNQETEKPVQPAEETQTNS
                     GKIANENTGEVSNKPSDSKPPVEESNQPEKNGTATKPENSGNTTSENGQTEPEPSNGN
                     STEDVSTESNTSNSNGNEEIKQENELDPDKKVEEPEKTLELRNVSDLELYSLSNGTYK
                     QHISLEQVPSNPNSYFVKVKSSSFKDVYLPVASISEERKNDKILYKITAKVEKLQQEI
                     ESRYKDNFTFYLAKKGTEETTNFTSFSNLVKAINQNPSGTYHLAASLNANEVELGPDE
                     RSYIKDTFTGRLIGEKDGKNYAIYNLKKPLFENLSGATVEKLSLKNVAISGKDDIGSL
                     ANEAQNNTKIKQVHVDGVLAGERGIGGLLAKAEQSSITESSFKGRIINTYETTAAYNI
                     GGMVGHLTGDKALLTKSKATVAISSNTNTSDQTVGGLAGLVDRDAQIQDSYAEGDINN
                     VKHFGRVAGVAGNLWDRTSGDVRHAGSLTNVLSDVNVTNGNAITGYHYNEMKVKDTFS
                     SKANRVYNVTLVKDEVVSKESFEERGTMLDASQIASKKAEINPLILPTVEPLSTSGKK
                     DSDFSKVAYYQAKRNLTYKNIEKLLPFYNKATIVKYGNLVNENSLLYQKELLSAVMMK
                     DNQVITDIVSNKQTANKLLLHYKDDLSEKLDLKYQNDFAKLAEYSLGNTGLLYTPNQF
                     LYDQTSIIKQVLPDLQKVDYHSEAIRKTLGISPNVKQTELYLEDQFAKTKQQLEDSLK
                     KLLSADAGLASANPVTEGYLVDKIKRNKEALLLGLTYLERWYNFSYGQVNVKDLVLYH
                     LDFFGKGNASPLDTLIELGKSGFNNLLAKNNVDTYGISLASQHGTTDLFSTLEHYRKV
                     FLPNTSNNDWFKSETKAYIVEEKSTIEEVKTKQGLAGTKYSIGVYDRITSATWKYRNM
                     VLPLLTLPERSVFVISTMSSLGFGAYDRYRSSDHKAGKALNDFVEENARETAKRQRDH
                     YDYWYRILDDNAREKLYRNILLYDAYKFGDDNTVGKATEVADFDNPNPAMQHFFGPVG
                     NKVGHNQHGAYATGDAVYYMGYRMLDKDGAITYTHEMTHDSDQDIYLGGYGRRSGLGP
                     EFFAKGLLQAPDHPDDATITINSILKHSKSDSTESRRLQVLDPTTRFNNADDLRQYVH
                     NMFDVVYMLEYLEGNSILKLDTNQKQQLLRKVTNEYHPDPDGNKVYATNVVRNLTVEE
                     VERLRSFNDLIDNNILSSREYASGKYERNGYFTIKLFAPIYAALSNDIGTPGDLMGRR
                     IAYELLAAKGFKDGMVPYISNQYEEEAKQKGKTINLYGKTRGLVTDDLVLEKVFNNQY
                     HTWSEFKKAMYQERQDQFDRLNKVTFNDTTQPWQTFAKKTTSSVDELQKLMDVAVRKD
                     AEHNYYHWNNYNPDIDSEVHKLKRAIFKAYLDQTNDFRSSIFENKK"
     gene            complement(24742..25920)
                     /locus_tag="ACL8CL_000696"
     CDS             complement(24742..25920)
                     /locus_tag="ACL8CL_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1329987.1"
                     /translation="MTLPVRKSLHNAVLQASEADTWEQATKEWNEVSLIFNGIGRSNC
                     VCGNVIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLERLVI
                     "
     gene            complement(26090..29740)
                     /gene="addA"
                     /locus_tag="ACL8CL_000697"
     CDS             complement(26090..29740)
                     /gene="addA"
                     /locus_tag="ACL8CL_000697"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HGS1329988.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMAERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVELKQHLSRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKETFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIDSLNQESDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADTYNEERKTQ
                     FAKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDTNHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRSRNDQILLALSEYGIPVKTDGEKNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKVHENLYEKLVNAQKMAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKTHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLEAKQYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTVLGQEILANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
     gene            complement(29737..33012)
                     /gene="rexB"
                     /locus_tag="ACL8CL_000698"
     CDS             complement(29737..33012)
                     /gene="rexB"
                     /locus_tag="ACL8CL_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HGS1329989.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKVSRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQTFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQTEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKRLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDQQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLHPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKNLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLKKLDLADSKRLVGEKLKQAWLEKIREE
                     LNR"
     gene            complement(33212..33367)
                     /locus_tag="ACL8CL_000699"
                     /pseudo
     CDS             complement(33212..33367)
                     /locus_tag="ACL8CL_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            33465..33848
                     /locus_tag="ACL8CL_000700"
     CDS             33465..33848
                     /locus_tag="ACL8CL_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS1329990.1"
                     /translation="MFVSYFNLLFSIDYINCLSETTDKNWHKSDRIFVTNTGKPVHSS
                     ILSKSLQRANERLKKPIPKHLSPHIFRHTTISILSENKIPLKTITDRVGHSDSEVTTS
                     IYTHVTKNMKDEAINVLDKVMKKIF"
     gene            complement(33986..35290)
                     /gene="eno"
                     /locus_tag="ACL8CL_000701"
     CDS             complement(33986..35290)
                     /gene="eno"
                     /locus_tag="ACL8CL_000701"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-displayed alpha-enolase"
                     /protein_id="HGS1329991.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            35454..35900
                     /locus_tag="ACL8CL_000702"
     CDS             35454..35900
                     /locus_tag="ACL8CL_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HGS1329992.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(35897..36996)
                     /locus_tag="ACL8CL_000703"
                     /pseudo
     CDS             complement(35897..36996)
                     /locus_tag="ACL8CL_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(37005..37334)
                     /locus_tag="ACL8CL_000704"
     CDS             complement(37005..37334)
                     /locus_tag="ACL8CL_000704"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HGS1329993.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVEQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCAKEVLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(37279..37653)
                     /locus_tag="ACL8CL_000705"
     CDS             complement(37279..37653)
                     /locus_tag="ACL8CL_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HGS1329994.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVCGVW
                     WIPIVGEISKIPWYCLFHCQPA"
     gene            complement(37712..39145)
                     /gene="glgA"
                     /locus_tag="ACL8CL_000706"
     CDS             complement(37712..39145)
                     /gene="glgA"
                     /locus_tag="ACL8CL_000706"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HGS1329995.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGFERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(39142..40281)
                     /gene="glgD"
                     /locus_tag="ACL8CL_000707"
     CDS             complement(39142..40281)
                     /gene="glgD"
                     /locus_tag="ACL8CL_000707"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HGS1329996.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAETFNMSTDIFVVDTPWLIEHLEEEAKKEHPEK
                     FRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GEGAQVEYAILDKGVEVEHGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(40271..41413)
                     /locus_tag="ACL8CL_000708"
     CDS             complement(40271..41413)
                     /locus_tag="ACL8CL_000708"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HGS1329997.1"
                     /translation="MKNEMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGTVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(41403..43331)
                     /gene="glgB"
                     /locus_tag="ACL8CL_000709"
     CDS             complement(41403..43331)
                     /gene="glgB"
                     /locus_tag="ACL8CL_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HGS1329998.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAILTELPEKKWKDGLWLARRKRWGFEERPVNIYEVHAGSWKRNSDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFASLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT        12569 a         8743 c         8617 g        13581 t
ORIGIN      
        1 gcggaggcct tgctagaaaa agtaacagat cctagtatta gacaaaatgc tatggagaca
       61 ttgactggtc taaaaagtag tcttcttctc ggaacgaaag ataataacac tatttcagca
      121 gaagtagata gtctcttggc tttattaaag gagagtaagt aaaggtagca gcattttcta
      181 actcctaaaa acaggatagg agaacgggaa aacgaaaaat gagagcagaa tgtgagttct
      241 agttctcatt tttttcatga aaatgtgcaa aatatagtag attgaaacta gaatagtata
      301 cctctacttc taaaacattg ttagaaatcg atttgattgt cctgttctta tttcatttta
      361 ctatatctta acagatagtg taaataaaga taaactattt actggctaat taatcagtta
      421 aacactagtt aaggagtaat gatgaaaaaa agaacaatac tattattgat ggccagtctg
      481 ttagctcttg tcttaggagc atgtggtttc ttggacatat tgttcctgga tcattctcat
      541 caggattact atttactgct attttagaaa ctggggtggt ttgatggaaa gtattggtct
      601 tgttatcgtt tcacattcca aacatattgc agaaggtgtt gttgaactaa ttagtaaagt
      661 agctaaagat gttccgatta cttatgtagg aggaaccgag ggcggaggaa ttggaacgag
      721 ttttgatcaa gtagataggg ttgtttccga aaatccagca gatactttac ttgccttttt
      781 tgacctaggt tctgctaaaa tgaacttaaa aatggtgact gatttcagtg ataaaagtat
      841 catcatcaac agggttccaa ttgtagaagg tgcctataat gcagctgctc ttcttcaggc
      901 tggtgcagaa ctgtcagtta ttcaaacaca gttagcggag cttgaaatca ataaataagg
      961 aattttacta taactctttt tataggtaag ctattgatta tctcaactat aaaatgttaa
     1021 gtaaatgatt tcaaaatcag aaagggattg ctttatgcag ttccttttta ttttaacagg
     1081 agtgaaacta tagtgtttct aaattgtgaa tcaatcaaaa ctgattgtga tggggctatt
     1141 ctagctttag aatccttcaa aaattaaaat ttaaggcaat caattacttg gaagagtatg
     1201 aaagcattta gtttatagga attctaggtc tagaattaca tatgtattta tgaagacggg
     1261 gtgttcgata gttagtattg ttctattctg aaagatttga gctgtcagtt gtatagaaag
     1321 tgttcgaatt tttttaagtg attaaattag ttaattgtat gaggtgcttt atgatataat
     1381 gttcttaatg aattttcaga aaggaaaacc tcaaattgtt ctacaaattt ctactcttcg
     1441 acctcgacca cactcttctt gattttgatg ctgctgagga tgtggctttg acccaacttc
     1501 taaaagaaga aggagttgcg gatattcagg cttataaaga ttattacgtt cctatgaaca
     1561 aggctctctg gaaagacttg gagctgaaga aaatcagtaa acaagagttg gttaacacgc
     1621 gcttttctcg tttattttct cattttggac aggaaaaaga cggtagtttt cttgcccagc
     1681 gttaccaatt ttatctcgct cagcagggac aaacactatc gggcgctcat gatctcttgg
     1741 acagcctcat tgagcgtgat tatgacttgt atgctgcgac aaatggcatt actgccattc
     1801 agacaggacg tttggctcaa tctggtctag taccttattt caatcaagtc tttatctcag
     1861 aacagttgca aactcaaaag ccggatgctc tcttttatga aaagattggc cagcaaattg
     1921 ctggatttag taaagaaaag acgctgatga ttggagattc tctaaccgcc gacattcaag
     1981 gtggcaataa tgcggggatt gacactatct ggtataatcc tcatcacctc gaaaatcaca
     2041 cacaagccca gccgacttac gaagtctatt cttaccaaga cttgctggat tgtttagata
     2101 aaaatattct tgaaaagatc acattttaaa ggagacgagc taatgactac aaaaaagcta
     2161 atattactat tgaagagtac aatgaagctg ttaattatga atttacatct gacacttgtc
     2221 aattagcaaa tagtatttat caatctcttt ttaagttttt tgataagaaa aatttctctg
     2281 gcgatttaat ttttacttgg aaatctccat cattagtcaa agaaggggat tatattggga
     2341 gaagggattc acaagtagat aatcttagag taataggaaa tatatttccg aattatctta
     2401 ctaatcgaaa atatagcctc aatatgaatc gtaatggctg tatgggagat tttcctcatg
     2461 acttttttga tatataccta gatcatgtag caaaatatgc ctacgaacaa aaagttaata
     2521 atattaaaga gtattatcct ttaaaaagag cgattttaca ccaagagaat gcattgtatt
     2581 ttcgattgtt ttctaatttt gacgactttt tagaaaaaaa ttatttaaag actatatggc
     2641 aagtttctaa agaaactcct ttttctgaaa tggattttaa tatgtttaaa aatatttcag
     2701 aaaagataat cttcgaaata ggtagtaaaa tgcttaatga tttaaaaagt aattataaaa
     2761 aatagccagt ctttataaac tgtattcacg tattcaaatc gtatatatag tcgatttatt
     2821 aaatctttag ataaactgta aaaagtggtt tccatagcca ctttttgcta taatagaggc
     2881 agtaaaaatg aaggagttgg ctatggaaca ctcgtcttgg cacgctttga ttaaggcgca
     2941 attacctgag ggttatttcg ggaaaatcaa tcagtttatg gagcaggtct attctcaggg
     3001 gactatttat ccacccaagg aaaaggtttt tcaggctctc ttgacaacac tgcttgaaga
     3061 agttaaggtg gtaattctag ggcaagaccc ctaccacgga ccaggtcaag cgcagggctt
     3121 gagtttttct gtacctgact ctatcccagc tccaccatcc ttgcaaaata tcttgaaaga
     3181 attgtcagat gatatcggag ttaagaaatc tcatgatttg acagcttggg ctgagcaagg
     3241 agtcttgctt cttaatgctt gtttgactgt tcctgctgga caggccaatg gtcatgctgg
     3301 tcaaatctgg gagcctttta ctgatgctgt gattcaggtg gtcaatcatc tagatagacc
     3361 agtcgttttt gtactctggg gagcttatgc acgtaagaag aaggcattag ttaccaatcc
     3421 tcatcacttg attatcgaat cagcccatcc aagtcctttg tcggtttata gaggattttg
     3481 gggttccaag cccttttcca aggccaatgc attcttaaaa gagacaggac aagagccaat
     3541 cgattggctt agataaggag aaaatatgcc tcagttagcg acgatttgct acattgataa
     3601 tgggaaagaa ctgctcatgc tccatcgtaa taagaaaccc aatgatgtcc atgaagggaa
     3661 atggattggt gtgggtggta agctagagag aggagaaacg ccccaggaat gcgcggtgcg
     3721 tgaaatcctt gaagaaacag ggctcaaagc caagccagtt ctaaaaggtg tcatcacttt
     3781 tcctgaattt acaccagatt tagactggta cacctatgtt tttaaggtga cggagtttga
     3841 gggcgacttg attgactgca atgaggggac gctagaatgg gttccctatg atgaggtttt
     3901 gagcaagccg acttgggaag gtgaccacac ctttgttgag tggcttttag gggataaacc
     3961 cttcttttca gccaagtttg tttatgatgg ggataaattg ttggataccc aagttgattt
     4021 ctatgaataa aggagaaagc agatgctact aatcaaaaat ggtcgtgtaa tggatcctaa
     4081 gtctggtttg gatcaagttt gtgatgtctt agttcaagat gggaaaatta tcaaaattgc
     4141 gcctgagatc acggaagaag gagcagaaac gattgatgct actggtcttg tggttgctcc
     4201 tggtttggtc gatattcatg tgcatttccg tgaacctggt caaacacata aagaagacat
     4261 tcatactggt gccctggcag ccgctgcagg tggttttact actgtcgtca tgatggctaa
     4321 taccagtcca accatttcag acgtggagac tttgcaagca gttctccagt cagctgccaa
     4381 agagaagatt aatgtcaaga cagttgcgac cattactaaa aactttaatg gtaaaaactt
     4441 gactgacttt aaggcgctct tagaagctgg tgcggttggt ttctctgatg acggtattcc
     4501 gcttgagagc agtaagattg tcaaggaagc catggaggaa gccaaaaaac tcaatacctt
     4561 tatcagcctt catgaggaag atccaggttt gaacggtgtt cttggcttta atgaaaatat
     4621 tgctagagaa catttccata tctgcggtgc tactggggtg gctgagtacg ctatgatggc
     4681 gcgtgatgtc atgattgcct atgcaactaa agcccatgtt cacatccagc atttgtctaa
     4741 ggaagaaagt gttaaagtag tggagtttgc tcaggggtta ggtgcagaag tcacagcaga
     4801 agtagcgcca cagcatttct ctaagacaga agcacttctt ttaacacaag gtagcaatgc
     4861 taagatgaat ccaccgcttc gtttggaatc agaccgtcgt gccgttatcg aaggtctcaa
     4921 atcaggtgtc atcacagtta ttgcgactga ccacgcgcct catcatgtag atgaaaaaaa
     4981 tgttgaggat attaccaaag cgccatctgg tatgactggc ttagaaacat ccctgtctct
     5041 cggcttgacc tatttagtag aagctggtga gttgagcttg atggaattac ttgaaaaaat
     5101 gacatacaac ccagccaagc tttacaactt tgaagcaggt tacttggctg agaatggtcc
     5161 agcagatatc actatttttg atgccaaggc tgaccgcctt gtggactccc attttgcttc
     5221 caaagcagct aattcaccat tcatcggtga aaccttaaaa gggcaggtta aatataccat
     5281 ctgtaaggga caaatcgtct atcaagcttg ataggagtta gaatatgaat tattttcgaa
     5341 aacgtaggga gagacaagcg aaatctaata gtggaattta ctgtcccgca gctaatcgtt
     5401 ccaaaattca atatgaaact aaagaaaaag ctgaccatgc ggttcaatat gataacggag
     5461 gtttagttag aagctattat tgtcgtacat gtgcgtgttg gcatactact tcaaaatcta
     5521 ataaacctcc gctaagtttg aaaaactatg gtctgaaact ctttggttta gataaatcag
     5581 aatagaaaga gaagtatgta taagacaaag tgtttacgag agaagttagt attattttta
     5641 aaaattttct tcccaatcct gatctaccaa tttgccaatt attctgcctc ttttgttgat
     5701 actgcaatga caggtcaata caacactatg gacttggctg gtgtatctat ggcaaccagt
     5761 atctggaatc ctttctttac atttctaaca gggattgtgt cagccttggt gcctatcatt
     5821 ggtcaccatc ttggtcgagg caaaaaggaa gaagttgcgt ctgattttta ccaatttatt
     5881 tatttggcct tgggcctatc tgtggtcttg ctggggatgg tacttttctt ggcaccaaca
     5941 atcttgaatc atattgggtt agaagcagca gtagcggcag tagcggttcg ctatctttgg
     6001 tttttatcta tcgggattat ccccttgttg ctctttagcg tcattcgttc cttgctggat
     6061 tcgctgggct tgaccaaact gtccatgtac ctcatgcttt tgttactccc tctcaatagc
     6121 ggatttaact atctcttgat ttacggtgcc tttggtgttc cagaactggg aggggctggt
     6181 gctggtttag gaacatcctt ggcctactgg gtcttgcttg ggatttctgt tctggtttta
     6241 tttaaacagg agaagctcaa agccttacac cttgagaaac gaattccact taatatggat
     6301 aacattaagg aaggagttcg tttaggtctg cctattgggg gaactgtttt cgcggaagtg
     6361 gctatctttt cagtggttgg cttgattatg gctaagtttt cgtccttgat tatagctagt
     6421 caccagtcag ctatgaactt ttcaagtctt atgtacgcct ttcctatgag tatctcatcg
     6481 gctatggcta ttgtcgtttc ctatgaagtg ggagccaagc gatttgatga tgcgaaaacc
     6541 tatattggtc taggaagatg gactgccctc atttttgcgg ccttcacctt aaccttcctt
     6601 tacattttta ggggaaatgt ggccagtctt tatggtaacg acccaaaatt tatcgatttg
     6661 acagtgcgtt ttttaactta tagtcttttc ttccagttag cagatacctt tgcggcgccg
     6721 cttcagggaa ttttgcgggg gtataaggat acagttattc ctttttacct tggtttgctt
     6781 ggttattggg gcgtagcaat ccctgtggct acgctatttg attctctaac agattttgga
     6841 gcttattctt actggatcgg cttgattatt agtttgattg tgagtggggc gctctaccgt
     6901 tggcgtttaa ctgtgattat gaagagattt gaatctttag caaaatccaa acgctaaaaa
     6961 gtttgtgaaa aaatattctt gataagaaaa tcccttgttt tcacggggtt ttctttttta
     7021 ttttgtgaaa tttcttttag aaaaactttg acagtatttg ccaaaaacgg taaaatagta
     7081 aggtaagaag aaagttagaa aggcaagaag atgtcaactt tagataaaaa tcttttgcta
     7141 gaaatgttcc gtaagatgga agaaatccgt cgcatggact taaaaattgc acaattagta
     7201 aagaaaggga aagtgccagg aatgacgcac ttttctgttg gtgaagaggc agctaacgtg
     7261 ggggctatgt tagctctcaa tccagatgat ctgattacct caaaccaccg tggacacggg
     7321 caagctattg ctaaagggat tgacctcaac ggaatgatgg ctgaaatcct tggtaaatac
     7381 actggaacct gtaaagggaa aggtggatct atgcatatcg ctgaccttga tgctgggaac
     7441 cttggtgcca atggtatcgt aggtggtggt atggggatcg ctgtcggtgc agccctcagt
     7501 cagcaaatgc aaaataccgg taaaatcgtt gtttgcttct ttggagatgg tgcgaccaat
     7561 gaaggtgttt tccacgaagc agtgaacatg gcttctatct ggaacctgcc agtcattttc
     7621 tattgcatta acaacggtta cggtatctct gcggatatca agaaaatgac caatatagaa
     7681 catatccatc aacgtagcgc cgcttatgga attcctggaa tgttcatcga agacggtaac
     7741 aatgtcatcg atgtctatga aggatttcag aaagctgtag accatgttcg cagtggcaat
     7801 ggtccagtct tgattgaaag tgtaacttat cgctggctcg gtcactcatc atctgaccct
     7861 ggtaaatatc gtacgcgtga agaagtggaa ttgtggaaac aaaaagatcc aatcgaaaac
     7921 ctccgcaatt accttattga aaataacatt gcaagtgccg aagaattgga agaaatccaa
     7981 gcgcaagtaa aggaagcagt agaagcttct gttaaatttg cagaggaaag cccattccca
     8041 ccgcttgaat cagcctttga agatatttac gcagactaag gagaaagaga taaaatggaa
     8101 acaaaaacaa tgtccttccg tgacaccatt atccttgcta tgtctgagga aatgcgtcgc
     8161 gatgaaaatg tgttcttgat gggagaagac gtcggtgtct tcggaggaga cttcggtact
     8221 tctgttggaa tgcttgaaga atttggtcca gaacgcgtcc gtgactgtcc gatttctgaa
     8281 gctgccatct ctggagcagc agcaggagca gccatgacag gacttcgtcc aatcgtcgat
     8341 atgaccttca tggacttctc tgttattgcc atggacaata tcgtcaacca agctgctaaa
     8401 acacgttaca tgtttggtgg taaaggtcag gttccaatga ctgttcgttg tgcagctggt
     8461 aacggagttg gctctgccgc ccagcactca caatctctag agtcttggtt tactcacatt
     8521 ccaggactta aggttgtggc acctggaaca cctgcggaca tgaaaggcct gcttaagtct
     8581 tctatccgtg ataacaactc agttattata cttgagtaca agtcagaatt taaccaaaaa
     8641 ggggaagtgc cagttgatcc agactacaca attccacttg gggttggtga aatcaaacgc
     8701 caaggaacgg atgtaacagt tgttacttat ggaaaaatgc ttcgccgtgt ggttcaagct
     8761 gctgaagaat tagcagaaga aggaatttca gttgaaattg ttgacccacg tacccttgtt
     8821 ccgcttgata aggatatcat cataaactca gtgaagaaaa ctggtaaggt tgttctggtc
     8881 aacgatgccc acaaaacaag cggctatatc ggtgaaattt cagctattat ttcagaatca
     8941 gaagcatttg actatctaga tgcaccaatc cgccgttgtg caggagaaga tgtgccgatg
     9001 ccttatgcac aaaacctaga aaatgcaatg attccaacag ttgaaagtat caaagatgca
     9061 atccgaaaaa cttataacaa agaatagagt tgcataagat aagttatgta aataaaataa
     9121 agctgacgag aaactttgta tcttttaggt gcagagctct tcgtcattaa tatcttagat
     9181 tagagcacgg gctaaaagct cggaaaaaag ataaatctcc ttggcttcat cgaagccatc
     9241 gtcgatttcc tatttttcag tcgcttttga cgccctttgt atcataaata gaattggaga
     9301 ggtcatggct gatgacaagc taagagcgac tcctgcagct agaaagttag cagatgattt
     9361 agggataaac ctctacgacg tttctggctc aggtgcaaac ggtcgtgtcc acaaagaaga
     9421 cgtggaaact tataaagaca caaacgtggt tcgcatttcg ccacttgcaa aacgaattgc
     9481 cctcgaacat aacattgctt ggcaggaaat ccaaggaacc ggtcatcgtg gtaaaatcat
     9541 gaagaaggat gttttggccc tgcttcctga aaatatcgaa aacgatagca tcaaatctcc
     9601 tgctcagatt gaaaaagtgg aagaagtccc tgataacgta acaccatatg gtgaaatcga
     9661 gcgtattcca atgacaccaa tgcgtaaggt tattgcccaa cgcatggttg aatcttactt
     9721 gactgcgcca accttcaccc tcaactatga agttgatatg actgaaatgt tggctcttcg
     9781 taagagggtt cttgagccaa tcatggaagc aactgggaag aagactactg taacagacct
     9841 tctttcactt gcagttgtta agactcttat gaaacatcca tgcatcaacg cttcattgac
     9901 agaagatggc aagactatta tcactcacaa ctatgtcaat cttgccatgg cagttgggat
     9961 ggataatgga ttgatgacac ctgttgttta caatgctgag aagatgagtc tttcagaact
    10021 ggttgtagcc tttaaggatg ttattggccg taccttggat ggtaaattgg ctccaagtga
    10081 gttacaaaat tcaacattca caatcagtaa tttgggaatg tttggtgttc agtcctttgg
    10141 tccgattatt aaccaaccca actcagctat ccttggtgtc agttcgacaa tcgagaagcc
    10201 agttgtcgtc aatggtgaaa ttgtgattcg cccaatcatg agtttaggat taaccattga
    10261 ccaccgtgtc gtagatggta tggctggtgc taagtttatg aaggacttga aagagttgat
    10321 tgaaacgcca atctcaatgt taatttaaga gcaagagtat tcatttttaa gatatagata
    10381 aaggaaggaa gacatggcct tagaagtaat tatgccaaaa gccggcgtgg atatgacaga
    10441 aggacaaatc gtccaatgga ataaaaaagt cggagaattt gtaaaagaag gagaaatcct
    10501 tttggaaatc atgactgata aagtcagcat ggaattggaa gccgaagaag atgggtactt
    10561 gattgccatt ctcaaaggag atggtgaaac tgtccctgta acggaagtta tcggttacct
    10621 tggtgaagaa agggaaaaca tcccaacagc tggagcagca tcgccagaag ctagccctgt
    10681 acctgtagca agtacctcaa acgatgatgg taagagcgat gatgcttttg atatcgttgt
    10741 gattggtgga ggtcctgctg gttatgttgc agccattaaa gctgcccaac tcggcggtaa
    10801 ggttgccctt gttgagaaat ctgaacttgg tggaacctgc ttaaaccgtg gatgtattcc
    10861 aaccaagacc taccttcata acgctgaaat tattgaaaat atcagtcatg ctgcaaatcg
    10921 tggtatcgtg attgaaaatc ctaacttcac tgttgatatg gaaaaacttt tagaaactaa
    10981 atctaaagtt gttaatactc ttgttggtgg tgttgcagga cttcttcgta gctacggagt
    11041 tactgttcat aaaggaattg gtacaatcac taaagacaag aacgtcttgg taaatggttc
    11101 tgaattgctt gaaaccaaga aaatcattct tgctggtggt tcaaaagtca gcaagatcaa
    11161 cgtccctggt atggaatctc cacttgtcat gactagtgat gacattcttg aaatgaacga
    11221 agtgccagaa agccttgtta tcatcggtgg tggagttgtc ggtatcgaac tcggtcaggc
    11281 cttcatgaca tttggttcaa aagtgactgt tatcgaaatg atggaccgta tagttccagc
    11341 tatggatgcg gaagtttcta agaaccttcg cttgatcctt gagcgtaaag gaatgaccat
    11401 cttgactggt actaaactgc aagaaatcat tgaggaaaat ggtcaacttc gtatcaaggt
    11461 tgaaggaaaa gacgatatca tcgcaagcaa agctcttctt tcaattggtc gtatgccaga
    11521 ccttgaaggt attggagagg ttgagtttga attggatcgt ggttgtatca aggtcaacga
    11581 atacatggaa acttcagttc caggcattta tgcaccaggt gacatcaacg gtactaagat
    11641 gttggctcac gcagctttcc gcatgggtga agtttccgct gaaaatgccc ttaaaggaaa
    11701 tcatgcagtt gccaaattga atttgactcc tgcagccatc tacactctcc ctgaagtagc
    11761 agcagtaggt ttgacagaag aacaagcccg tgagaaatac gatgttgcca tcggtaagtt
    11821 taactttgct gctaacggtc gtgctattgc atctgacgca gctcaaggtt tcgtaaaagt
    11881 tatcgctgat aagaaatatg gagaaatcct tggtgtacac attattggtc ctgcagccgc
    11941 agaattaata aacgaagcat caagcatcat cgaaatggaa atcactgttg aggaaatgct
    12001 gaagaccatc cacgggcacc caacttactc tgaagtgatg tacgaagcgt ttgcagatgt
    12061 tctaggaatg gccatccatt cacctaagaa aaaataaaac gaagatagat tagactggaa
    12121 ggttatttcc agtctctatc atataaagta aagggatatc atgaaataca ttatcaatca
    12181 ttcaaatgac actgctttta atattgcctt ggaagaatac gcctttaaac accttttgga
    12241 tgaggatcaa atcttcctac tttggattaa caagccatct atcattgttg gtcgtcacca
    12301 gaacactatc gaagaaatca accgtgatta tgttcgcgaa aatggtattg aggtagttcg
    12361 ccgtattagt ggtggtggag ctgtttacca cgatttaaat aacctcaact acacgatcat
    12421 ctccaaagaa gatgaaaata aggcatttga cttcaagagc ttctcaactc cggttatcaa
    12481 taccctggct caactcgggg ttaaagctga gttcacaggt cgtaatgacc ttgagattga
    12541 tggtaagaag ttctgtggca atgcccaagc ctatatcaac ggccgtatca tgcaccacgg
    12601 ttgcttgctc tttgacgttg atttgtcagt cctcgcaaat gcccttaagg tttcaaaaga
    12661 taaatttgaa tcaaaaggtg tgaaatccgt ccgtgcccgt gtaactaata ttatcaatga
    12721 attaccaaaa aaaatcacag tcgaaaaatt ccgtgattta ctcttggaat acatgaaaaa
    12781 agagtaccca gagatgactg aatacgtctt ttcagaagaa gaattggccg aaatcaatca
    12841 catcaaggat actaagtttg gaacttggga ctggaactat ggtaaatcac ctgaatttaa
    12901 cgtccgtcgt ggaaccaaat tcactagtgg taaggttgaa gtctttgcta acgttactga
    12961 atcaaaaatc caagacatca agatttatgg tgacttcttt ggtattgaag acgttgctgc
    13021 agtagaagat gtccttcgtg gggtgaaata cgaacgcgaa gatgttctta aggcgctaaa
    13081 aaccattgat atcacacgct acttcgttgg tattagccgt gaagaaattg ctgaagcggt
    13141 agttggataa atcaaaaaga agttggccaa ctaaccaact tctttttttg atatttattt
    13201 acataattta aaatacgtaa aatcataaac tatccagagc attcttttgt tcatcactaa
    13261 caatatgggt atagaggtca gtgacttgtg tgctagcatg tcctagttgg tgactgacta
    13321 aaacttgtga tttagtcgca tcatagagcc tagttgctag tgtatggcgc agtttatggg
    13381 gtgttacacg cactttaaaa tcctctgagt atttagcaac cattttctca acgctagaag
    13441 catcgatacg attaggaaca cctctgtaga gagttaaaaa aagggctgta tctgtttttt
    13501 ccgttttata gcgttgattc cgaatggcca gataattctc taaataaggt ttagcaaaag
    13561 cagcgacatt gactgagtca cgtttgccac cttttcgagt aacatcaata accatcattt
    13621 ttagattgag atctcttaga tctagattaa cagcttcaga taagcgaaca ccagatgcca
    13681 agagaagggc aataatggct aaatctcgtt ctttattttt gttgaatgat gagagagctc
    13741 gatttgaaag ctgttgtggg tgctcttgat cgatataagt tagaaaacct gctgtttcat
    13801 cacctagaaa gagtttttgc ttgatatttt cagctctggc agcaagggtt tctttctttt
    13861 tcttggttga aacttttttc attacattac gatagaaata aggttccccc tgatcgtttt
    13921 caacctcctc ggttagatac ttgtaaagac tagaaagtgc tgataaggtt cgattgatag
    13981 ttgtctgtga aacaccttgt tttgttgtat tagcattcag caagggacgt tcacgtagat
    14041 aaaggataaa ggattccatg tctttcttag acatattttc caaaactgat aaaggaatat
    14101 cggatatttt atcagcgttt gaaataccag actccaaaac ccagctgaaa aatcggtcat
    14161 attccttaag atattcgtac agggttgtaa aactgtaggg cacagccagc ttagattggt
    14221 agtattccag aacataccag ggcatgagtt gttttagttt gtcgattcgt tccagtaaaa
    14281 tctcacgttt catgtatttt ctccataatt aagtatacta gtagtatagc atagacttgt
    14341 atgtttttca agaaaattat agtttttcag aaagatgtta taggagtttt tcaaagtgat
    14401 aaaaagactt agacctacaa acaaaacaga ataaggaaaa taataaaaat ctcacccact
    14461 aaacttctgt taatactaca taactgaaga gctatataag gagctatgag atacaaaaca
    14521 cattgtataa tagattgaaa ctagaatagt acatctggac ttctaaaaca ttgctagaaa
    14581 ttgatttgat tgtcctgatt gttttatcct atttctattt catttctcta tataattgct
    14641 attgttttct ttttcatgat tttaatctta ctgtacagat ttgatacagt aaactccttt
    14701 ctagctttat actttatcat ttctaactta attatagtct tatttttaat aaagttcaat
    14761 tatttatatg taaaattcct catcagtaaa aaataaccga tctcatttct gagaatcggt
    14821 tttctaaata aatcaaactg atcatttaca taacataaat tatgtaaatg atcatactac
    14881 aacaacaaat ctttgacttt tccaatttca ctttttggaa taaccaggtg aatcatatca
    14941 cccagataca ttctggttga gccgttaact gtttggctct tgccattatg gacttgagtt
    15001 gtgatgagga cgttgtgtgg taagttgagt tcatgaactt gtttcccagc aattttatca
    15061 gaaactggta tttcgataag tgtaacttct ccttcgctag atacttcttc tggaagcatt
    15121 ttttccagca tggcttcata gactggcgta cctttgagca aatccatgat aatataagaa
    15181 acaagagtga caagaccaag tggcataagg ttgcgaatat ctcctaccat ctcagttacg
    15241 aggatcatag cggttaaggg agcttttgat atggctccaa aatagccact cattcctaga
    15301 atgacaaata tagggaattg ctcttgactg acaagtccaa gattgacaca gataacacca
    15361 actaaggcac caagcaaaga accaagagct aaaatgggga ggaaaattcc tcctggcagt
    15421 ccacttccat agctaatcat actccaaata aagcggatta aaaagtaagc taataaaact
    15481 tggaaactaa aattttgttc agttaaagaa aggacaagct gatttccgcc accaatgatt
    15541 tgaggtaaga agattccgac tggtatgata aggataaaag ccaagatggg ataataagcc
    15601 ctatccaaat ggattttttg accaatcaag tcataaactc ttccaacgtt taatacagct
    15661 ttctcataga gaaaacctga aaatccaagg aaaattccca tgacgagata tatccaatac
    15721 tggtctaggg tcatgggagg aatgttatct ggcatatcca atactggtgt caaaccgaac
    15781 atgagtagag acacaaagtt tgctacgatg ctggctgcta gagttgagac ccagaaaaag
    15841 cgcgaaaaat ggtgatagac ttcttctaca acaaagagaa gtgctgcaat aggagcatta
    15901 aaggctgcgg ctaaacctgc tgcagctcca ctggcaatca aggaacgttc ctctactgga
    15961 ctggatttga gccacttggc aattccttta ccaccaactg ctccaagttg aatgctgggt
    16021 ccctctcgac ccagcatgag tccactggca atagcaagaa tacctagcac atattttttc
    16081 caaagaatgc cccaccagtt gagggacatg aggcctttca gttcggcttc gacttgagga
    16141 attcctgagc ctttaatatc tttttctgac cgtgttagtt tggcactgag ccaacagatg
    16201 agtatataaa acaaaaccag tacaaaaaga ttgcgcacta agtacccttg atcttgataa
    16261 actccttgta tcaggtggaa gcccttttca attaagaaac gaaaggatcc gacgataatt
    16321 ccaacgatga ggccgacaat gattcctcga ccaacttggg atagtatagt gctggatgca
    16381 aaggcgaatt ctttcttaga acggactatt tccgactgtt cctccatggt atttcctttc
    16441 aattaacttt cttttttacc taaaaccagt gatttaacgg gttcaaagct ggttcggtga
    16501 attggggtaa ctcctagttt tgtgaggcct tccagatgtt tagctgtgcc atatcctgca
    16561 ttagtagcga aatcatagcc agagaactgc tgatcgtatt ctttcatcaa ttcatcacgt
    16621 gttaccttgg ctactataga tgctgctgcg atagagaggg agttggcatc tcctttgata
    16681 atggaggttt gtgaaatggg caagtccagt ttcatggcat caatcaaaag gtgctctggt
    16741 tgagggctga gctgggagat tgcttcttgc atggctagtt tggttgcttc atagatgttg
    16801 acttggtcga tgacctgatt atctatgata ccaattccaa tcgacaaggc ttggtcttga
    16861 acggcttgga aaatctccag atgtttcttt ttaggaattt tcttgctgtc gttgagacct
    16921 ttaatcttac aatttttagg taaaataacg gccgcagcga ctacaggacc agcaagagga
    16981 ccacgaccaa cctcatcaat acctgcaatt aaggtcaatc cttgcttata aagttctttt
    17041 tcataagaaa gcatggattc caagcgcaaa ttttcatcta attcagcttg aatggctctt
    17101 ttacgcttgc tgatttcctt ttgaactcct gagcgattat ccttttcaag ctctaaaaaa
    17161 atagggcttt ctaactcctt gactgtcaca aggagttctt tgatttcttt aatcgttgcc
    17221 atcgaggtct tccaatgtat ctaaggtata gttaccgagt ttgccatcac ggacttcctt
    17281 cacgaagaga ctgtaaaaac ggtcatagtc atcacggaaa ccgagggcgc gggtcatatc
    17341 cataataatc acaggcgctt cttcttcaat tttcatttgt ttgaagcgtt cagccagctt
    17401 ttctggataa tgttctttga aataattgat accaaaaatg gtaacctcat ccataggaag
    17461 caactgatcc ttgatagctc cagtcaatgc caacttaagt gcaacagttt catcctcaaa
    17521 cttaggccag agaatccccg gtgtatccaa gatttccaga tctttattgg ttttaagcca
    17581 ttgttgacct tttgtgaccc ctggcttgtt tccaacaaca gcaatctttt taccagccaa
    17641 acggttcatc agagttgatt taccagcgtt tggaatccca ataatcatgg tacgcaaggt
    17701 ttcaatctga atcccacgtt ctttctggcg agcaatctta tcagccatga gcttcttggc
    17761 cgcatctgtt acaactttta cagtcacttg ctctttggag ttgatagcta gcgtctggat
    17821 tccttgtgat tcaaaatact gacgccattc cttggtcatt gctggatcag ccaagtcggc
    17881 cttgtttaaa atcaagagtt ttggtttatc accaacaatc ttggtcaaca taggattttg
    17941 actagataga ggcaagcgtg catctactaa aatcgtcaca aaatcaacaa attttaaatt
    18001 ctcctgcacc tgtcgacgag ctttagacat gtgaccagga aaccattgaa tagtagccat
    18061 tgagtttttt cctttcatag caaggattta gatcccctat tttattttac tattgtttga
    18121 acatagagag ttttcaaatg aatgatgtcc aaacagttct tgttttatga catagaaatc
    18181 tactttatat tatatcatgc cttctttatt ttgtccgatg atagcctttt accctatctt
    18241 ttaccctatc ttttacccta tatagaaaaa agccatcaaa tgatgacttt tcagagggag
    18301 caaaaaactg ttcccgtcat attatgaacc aaaagaacta aactaccaac agctaatagg
    18361 cctaaagctg taagctcaga agttgatgaa cctgttgcag gaagagtttc ctgctgataa
    18421 ataagatcat aacttacttc attatcagta ggaattcctg cttgaatctt ggtttgttct
    18481 tcatcacctt tttaaacttt atttcctaat agtagacact attttttatt ctcaaaaatt
    18541 gaacttctaa aatcatttgt ttggtcaaga taggctttaa agattgctct cttgagcttg
    18601 tggacttcac tatctatgtc tggattgtag ttattccaat ggtagtaatt gtgttctgca
    18661 tccttacgaa cagcaacgtc cattaatttc tgtaattcat ctacactgct tgtagttttc
    18721 ttggcaaatg tttgccaagg ctgtgttgta tcattaaaag taactttgtt caatctatca
    18781 aactgatctt gtcgttcttg atacatagct ttcttaaact cactccaagt atgatattgg
    18841 ttattaaata ccttttccaa aaccaagtca tctgtaacca aacctcttgt tttaccgtag
    18901 agattgattg tcttgccctt ttgtttggct tcttcttcgt attggtttga gatatatggt
    18961 accataccat ctttaaagcc tttagcagct agtagttcat aggctatacg acgtcccatc
    19021 aggtcacctg gtgttcctat atcattactt aatgcagcat aaatcggtgc aaataactta
    19081 atagtgaagt agccatttct ttcgtattta cctgaggcat attccctaga cgaaagaata
    19141 ttattatcaa tcaaatcatt gaatgaacgt agtctttcaa cttcttctac tgttagattt
    19201 ctgacaacat ttgttgcata gaccttattt ccatcaggat caggatggta ctcatttgta
    19261 acttttctaa gaagttgttg tttttgattc gtatccaatt taagaattga atttccttcg
    19321 agatattcca acatataaac aacgtcaaac atgttgtgga catattgcct caaatcatct
    19381 gcattattaa atcttgtagt tggatcaagt acttgtaatc gtcgactttc tgtactatca
    19441 gattttgaat gtttcaagat ggagttgatg gtaatggtcg catcatctgg atggtctggt
    19501 gcttgtaata atcctttagc aaagaactct ggtcccaagc cacttcttcg accatatcct
    19561 ccaagataaa tgtcctgatc tgagtcatgt gtcatctcat gcgtataagt aatagctcca
    19621 tccttatcca acattcgata acccatataa taaactgcat cacctgtagc ataagcaccg
    19681 tgttgattat gcccaacttt atttccaaca ggtccaaaga aatgttgcat tgcaggattt
    19741 ggattatcaa aatctgccac ttctgtagct ttccctacgg tattatcatc gccaaattta
    19801 taagcatcgt aaagcaaaat atttctataa agtttttcac gtgcattgtc gtctaaaata
    19861 cgataccaat aatcgtagtg atctcgctga cgtttggctg tttcacgcgc attttcttca
    19921 acaaaatcat tgagagcctt gcccgcttta tggtcactac tgcggtagcg atcataagct
    19981 ccaaatccta gactagacat ggtcgagatg acaaatacgg atctctctgg caaggtcagg
    20041 agaggcaaga ccatattgcg gtatttccat gtggcactcg tgatacgatc ataaacaccg
    20101 atagaatact tggtgccagc taacccttgc ttcgttttca cctcttcgat agtggatttt
    20161 tcttcgacaa tgtaagcctt agtctctgat ttaaaccagt cattattgct tgtatttggt
    20221 aaaaagactt ttcggtaatg ttccagcgtg ctaaacaaat ctgtcgttcc atgttgactg
    20281 gcaagactga taccataagt atcgacatta ttcttagcta gaagattgtt aaagccagat
    20341 ttacccaact caatcagagt atctaatggt gaagcattcc ccttaccaaa gaagtccaaa
    20401 tggtacagaa ctaggtcttt gacattcacc tgaccatagc taaagttata ccaccgttcc
    20461 agataggtca agccaagtag caaggcttcc ttgttgcgtt tgattttatc tacaagataa
    20521 ccttcagtga cggggttagc actagccagt ccagcatccg ctgacaagag ttttttcaaa
    20581 ctgtcttcca gttgttgttt tgttttggcg aactggtctt ctagatagag ctcagtttgc
    20641 ttgacgtttg gagaaatacc cagcgtcttt ctgatggctt ctgaatgata gtcaaccttt
    20701 tgtaagtcag gtaagacttg cttgatgata gaggtttggt catacaggaa ttggtttggc
    20761 gtatagagaa gtccagtatt gcccagacta tattctgcta atttggcgaa atcattctgg
    20821 tatttgagat ccagcttctc agataaatca tccttgtagt gaagcaagag tttgtttgca
    20881 gtctgtttgt tagaaacaat gtctgtgatg acttggttgt ccttcatcat gactgctgac
    20941 aagagttctt tttgatataa aagactgttc tcattgacca ggtttccgta tttgacgatg
    21001 gttgccttgt tgtagaaagg tagcaatttt tcaatgtttt tataagtcaa gttgcgctta
    21061 gcttgataat aggccacctt agaaaaatca ctgtcttttt tgccacttgt tgaaagtggc
    21121 tccactgttg gtagaatgag aggattgatt tctgcttttt tgcttgcaat ttgagaagca
    21181 tctagcattg ttcctctttc ttcaaaggat tccttgctga cgacctcatc cttgaccaag
    21241 gtgacattgt agactctgtt ggccttgctg ctgaatgtgt cctttacctt catttcgtta
    21301 tagtggtaac cagtgatggc atttccgttg gttacattaa catcgctgag aacattggtc
    21361 aaacttccag catgcctaac atcaccagaa gttcgatccc acaaattgcc tgccactcca
    21421 gcgactctac caaagtgctt gacattgttg atatcacctt cagcatagct atcttggatc
    21481 tgtgcatctc ggtctactag gcctgcaagt ccacccacag tctgatctga agtatttgtg
    21541 ttagatgaaa tggctactgt cgcttttgac ttagtaagta aagccttgtc acctgtcaaa
    21601 tgaccgacca taccaccgat attgtaggca gcagtcgttt cataagtgtt gataattctt
    21661 cccttgaaac tgctctctgt gatgcttgat tgctcagcct tagccagcaa accaccgata
    21721 ccacgttcac cagccagaac accatcgacg tgaacttgct taatttttgt gttattctga
    21781 gcttcatttg ccagtgaacc gatatcatct ttccctgaaa tagcaacatt ttttagactc
    21841 agtttttcta ctgtagcacc actcaagttt tcaaacagag gttttttcaa attatagata
    21901 gcataattct tgccatcttt ttcaccgatt aaacgaccag taaaggtgtc cttgatatag
    21961 gatctttcat caggaccaag ctccacttcg ttagcattca ggctggccgc taaatgatag
    22021 gttccagagg gattttggtt tatagctttg accagattac taaaggaagt aaagtttgtt
    22081 gtttcttctg ttcccttctt agctagatag aaggtaaaat tatctttata tctgctttct
    22141 atctcctgct gaagcttctc tacttttgct gtgattttat aaaggatttt atcatttttt
    22201 ctttcctctg atattgatgc tactggtagg tatacatctt tgaatgaaga agatttcact
    22261 ttaacaaagt agctatttgg attgcttgga acttgctcta acgaaatgtg ttgtttataa
    22321 gtaccatttg acaaactgta taactctagg tcggaaacat ttcttaattc aagtgttttc
    22381 tctggttctt ctaccttttt atcagggtct agttcatttt cttgtttaat ttcttcgttt
    22441 ccatttgaat tggatgtgtt tgattcggtt gaaacatcct cagttgaatt tccgtttgat
    22501 ggttctggtt ctgtttgtcc attctctgat gttgtattac ctgaattttc tggttttgtt
    22561 gcagttccgt ttttttctgg ttgatttgat tcttcaactg gtggttttga atcactaggt
    22621 ttattggata cttctccagt attttcgtta gctattttcc cagagtttgt ttgtgtttct
    22681 tctgcaggtt gaactggttt ttctgtttct tgatttgagg taccttctac tgtgccttca
    22741 tttggattta ctggaacttc ttctacagtt ttttctgaat tttcattttt agagtcatta
    22801 tgttctggtt tatttgattc tccaactgag gttgtcgaat cactaggatt actggacact
    22861 tccccagtat ttttgctaga tgtatctggt gatactttct ctgaattcgt tgttgattct
    22921 tctgcaggtt gaactggatt ttctgcttct tgaattgagg ttccttctgt agtaccttca
    22981 tttggattta ctggtgtttc ttctgttggt tttactggaa cttcttcagt tttttctgga
    23041 ccttgttctt tggtcttctc aaccggagtt tcaggtttta cttgctcaat attaccctta
    23101 tattctggaa gcggtgctac ctgctctggt tcacctttat cacttaccac agtatctggc
    23161 gactctggtt gaacctcagt ctcacctttg tcggtcacaa ctgcttcggg taatgtaggt
    23221 tgaacttctg gttcgccttt gtcacttact acagcttcgg gcaactcagg ctgaattgcg
    23281 ggttcaacaa tagctccaga ctgtacgtcc ttatgttcta caccagtctc aggttgttcc
    23341 tttataactt gagttttttt agtacctttt tcgactattc ttggactagg cgcagtcgtt
    23401 gaagttgaaa caatttctcg cgaaacttct tccttgttta cagagaatat tctgacgatt
    23461 tcaactttct tacctaattt accttcttgt tttactctta cagttccttc agctaaatca
    23521 ggattttctt gaatttcttc ttgaaaatct atttttgtct ctatagtttc ctcacgatat
    23581 aagagttcag gtttgttcaa ttgacctgat aaaacttcat cctgtggatt taatgtattt
    23641 accccagtct tttcttttgg agaaatcttc tcctctttct tcgtttctag attcttatgt
    23701 tcggctaatt gttcttgaga atctgaagat tgtttctctt cttttcttgg attgattaat
    23761 tcagtagaga aaggtttttc aactacttga acttctgtcg gcttagttga agaaacaggt
    23821 gtttgttcct gaatagcttg tactgttgat ggatggtcta caaaattcgg tgtaacatta
    23881 taatccacct tttgttgttt tgtaggagtg gcaactgaac tcttttgatt acttacttca
    23941 gactcagaag tcgtttttcc ctctttgata tatccaatat aagtgtaacc tgaaatctct
    24001 ttaggaagag gtaatttttc tccagaggtc aattcatagt ccgtattgta atttagcaaa
    24061 agatgatttt ctaaagcatg gactgaaact aagacaccat ttcctatccc tgcaaccaat
    24121 actaaatgta ataccgtttt attcttaacc tttttcttgg aaacagcaaa aattaaaatt
    24181 cccatagcag ctaagctagc accagcaact agggcttgcc tctcattctt gcttccagta
    24241 tttggcaatt ccgccagttg attttgagaa tttaacttat aaacaagata ataagtttca
    24301 tcatcattct ccacgtatgt cggaatatca tagacaagct gcttcttttc ttctgatgat
    24361 agctctgaat ctgccacata tttatagtga actcccgcag tttcttgagc atccacagat
    24421 gaactagcta atacagacat aaaaaataaa cttgaaatcg ttgcagatac aagtcctact
    24481 gataattttc taaatgaaaa acgctcttgt ttttcaccaa aatacttttc cattattcct
    24541 ccttgaaata aaatttatat atgttacaaa gacctttatt atattagtgt attatctatt
    24601 atctatagaa aaggcagtat accttaatta tactcttaat ttacaaaaaa gtcttaaaat
    24661 tgagatgcgc tttcatactt tgttttatat tatttggagg tacaataaca cctaccatga
    24721 aatttacacg gtaggtgtta ctcatatcac taatcgttct aaaaatggtt tgaggcagtt
    24781 gaggagaatt ccttctatcc agcttccttg tgctgatgag cgatggtctt cctgcaggct
    24841 tttttttaga aaatctcgga cttgttctgg tgcgatttca aattcaaagg ctttcatttt
    24901 atagaaaaag tcgatgagat gatctgacag gtattcagtt gaaaagggta cttcaccact
    24961 ttttctatat tctaataaga gtctagaaaa tcgagctttt tcttcaggaa gctcacgaaa
    25021 ataggaattg aggagccaag tctgcttctg ttttctttca attggatcct gactggcaat
    25081 tcgttggtct ttttccagct ctttttggta ttgtttggcc ttgatagccc gttctgctct
    25141 atttttacca aaaagaattt tttcccactt gcgttcttct tgagtcaggg tctctgtaaa
    25201 gccaaagtaa tcttgataag cacgctctgc gggtcccatg gctagaacca gattgtctgc
    25261 atattgcttg gcgattttat ccctcttctt gcgttctttc tctgcctgga tacggagttc
    25321 ttgttcgtag tcaattttct ccttgcctag ctttacaagg tagagttggt catccgattt
    25381 cccaagtaaa aagggtttga tacacttttc aaggacttct tccatccgag cctttttctt
    25441 tggttccgcc ttggtccaac ttcctccctg aaagacttct aggaaaagct ggtagtctct
    25501 ctcaggcgca aattgattgc cacgattggg tttgaaaaca cctttttccc agagccattt
    25561 tagaagtcgc tcgtcaaagt cacttttatt gaccttgatt ttttcctttt tctgagcttt
    25621 tctggttaga ttttcaacct ttctgagcag tttttcttcc tcttccaatt gctggtcaag
    25681 ggacaatcga tgaaaatgac gaacacagtc gctaccaatt ggaaagaggc gttggcctgt
    25741 gacaccgtta aagagttcat aagcgtattt gatgacattt ccacagacac aattgctacg
    25801 gccgataccg ttaaaaatca aggaaacttc attccattcc ttggtagctt gttcccaagt
    25861 atccgcttct gaagcctgta aaaccgcatt gtgtagggat tttctaactg gaagtgtcat
    25921 gaggtctcct ttctaatact caataaaaat caaagagcaa actagaaagc tagccgcagt
    25981 cagctcaaaa cactgttttg aggttgtgga tagaactgac gaagtcagta accatatata
    26041 cagcaaggcg aagctgacgt ggtttgaaga gattttcgaa gagtataagt tatactttta
    26101 caacttgaac ctcgtcttta ccgagtaaaa tcaagtattt ttcaatattt tcaatcgaat
    26161 aggctcgtga taaagcctct tcgtatagag ctaactgacc acgatagcgg tctacgagtt
    26221 gacttggttc atcatagcgg tctgtcttgt agtcgaacag aacaattttg ttttcgtaaa
    26281 gcagatagcc atcaaggata ccacggacaa caaagtcttc ctgactcttt tggtctcgtt
    26341 tgagcatgga gaaaggttgc tcgcgataga gatggtcggt attagcaaga atttcctgac
    26401 cgagtactgt gtcaaagaaa gcaagaattt tatcaagatt gatcttgtct ctgacagctt
    26461 ggctagtttg aacttgtttg agtgtttctg ttaggctagc aagggttagt tgctggctga
    26521 ggtcaattct ctgcatgagt tcgtgagtag cactaccaat ctcagctcca gttacctttt
    26581 cttttgttga aaaatctggc aaatcgaagc tgattttctt gcctactgac tgaccttgac
    26641 cagcaatctc gacaccttcc atatccataa ctggttcgta gaatttcttg atttgacttg
    26701 gggtttgaac actaggaagt tcaatagctg cgcggtgaag agtattataa acttccacct
    26761 ccttcagcat ttccagagct tctttgatgg tatctgactg acgattgtct gcttgggagc
    26821 tatcttggag aggactcttg gtttccaact ctccgatagc ttctctggtc aactgatctt
    26881 cgccaataaa acgataacta aagttgagct tgtccttagt aaacacttta ctgatagccc
    26941 aaagccaatc ttggaaattc cgtgcttgca gtctagtatt gctatttagt ttcccatttt
    27001 tggctgctgg gtattgcttg gcttccagct tttcacgaga acccttgccg acaagataga
    27061 gctttttctc agcccgcgtc atagcaacat acagcaaacg catctgctca gaatagcttg
    27121 ctagctgtaa ttcctcttca ttctgcctat aggtcaggct aggaatggag agtttgatgg
    27181 ttttaggata gtggtcttct actgcccctg tctccatctt ggcaatatat ttgacaccaa
    27241 gaccattctg acgactgaga atgacttctg acatagagtc ttgcttgttg aaatcttgat
    27301 ccatattgag gataaagacg taaggaaact ccagcccctt actcttgtgg atggtcatga
    27361 gctctactgc atcttttggc ggtgcgacgg ccacgcttgc caaatcgtgc tgggcttcta
    27421 agacttggtc aatcatacga ataaaacgcg ataaaccttt gaaattgctc ttttcaaatt
    27481 gatcagcacg cagtgctagg gcatagagat tggcctgcct agcaggacca ttcggcaaag
    27541 ccccaacata gtcataataa aaacggtcgt tgtaaatctt ccaaatcaag tcatagagag
    27601 agtgggtttt ggcatacaag cgccaagaag ctaggatatc catgaattgc tttagttttt
    27661 cagctagagc tgtgtgaatc aaaccttttt gactacttgc cattttttgt gcattgacca
    27721 gtttctcata gagattttcg tggactttat cctctgcttt ctgaagggac aaacgtgcta
    27781 gctcatcctc atcaaaacca aacattggag acttcataag ggcaaccaag gcgtagtctt
    27841 gcaggggatt gtgaatgaca cgaagagtgt ctagcatgac ttgcacttct agggattgga
    27901 gataattgtt tttctctccg tcagttttga caggaattcc gtactcagac agggcgagga
    27961 gaatctggtc attacgactg cggctggagg tcagaagggc aatttcctta aaggcaacac
    28021 ctttttcttg atgaagtttc agaatctcct tgataactaa gcgcatttcg cctgttagtt
    28081 tcgtttctgt ttgactctct tcttcctcac ctgtatcgtc cttatcgtag aggagaaatt
    28141 cggccttgtt gtctggattg ggagtcagtt tggtattggc aaaaacaagc tggtgcatgt
    28201 tatcatagtt gatttcgccg acctcttggt ccatgagacg ttcaaagaca tcattggttg
    28261 ctgacagcac ttctgaacta ctacggaaat tttccttgag gagaatgagc ttgccttctt
    28321 gaggattttg cgcatagcgt tggaatttct cattgaaaat ctgcgggtct gcctgacgga
    28381 aacgatagat ggattgcttg atatctccca ccataaagcg attgtggcca ttagacaaca
    28441 attccagcat ccgttcttga atatggttgg tatcctgata ctcatcgacc atgacttcat
    28501 ggaagcgctc ctgataagac tcacgaactt gtgggaaatt ctctaaaatc tcaatggtgt
    28561 aatggctgat atcagcgaat tcaaaggcat tttcctgtcg ttttctctga cgataagcct
    28621 ctacaaaatc gctcatgaaa gattggaagg ttttagctag tttccaagtg tctccatgat
    28681 aacgttcttg atagtcgaga atcgctatct ggtctgataa ttgtcctagt ttagcaaact
    28741 gggtctttct ctcttcgttg taggtatcag ccaggggctt caaatcagcc ttacggctgg
    28801 cattagtcag agctcgaccg tttttctcct tagagatggc gacaacacgc gcaagcactg
    28861 cctgataagc ctgactatcg gactcctgat ttagggagtc aatttcatcc agaattaact
    28921 gaacattttc taaataggca gcctttgcaa actccttggc atcgttatcc agatggtaac
    28981 ggaaaaagct ttccaaatcc caaagggctt gtttgatttg ctcagtcagt ttttcttttt
    29041 cactggtaaa atcagctttc tcaaatcctt tgaggaaaga ttcactcagc cacttttgag
    29101 gattactggt ggattggagg aagtcataga ttttatagac ctgctggcgc agaccccgtt
    29161 cgtccttgcc acgcccagca aagtttttca gcaaatgact aaaggtctct ttctgtttac
    29221 cttggtaatg cgcttcaaag acctcatgaa agacttcgtt ttcgagaaga agttgctcgc
    29281 tttggttttg taaaatacgg aaattaggtg caatatcaag cagataacca tgtttgccaa
    29341 ggaatttttg tgtgaaagag tccatggttc cgatggcagc gttgggtagg tctgccaact
    29401 ggcgactcaa gtgttgtttg agctcgacat catctgtttc ttggattttc ttgctgattt
    29461 ttttctctaa acgttcttta agttcagttg cagccttgac ggtaaaggtt gagataaaga
    29521 gttgagaaat ttcgacacca cgcgccaatt ggtccagaat gcgctctgcc atgacaaagg
    29581 tctttccaga accagccgat gctgagacca ggatattctg gccagaagtg tagatagctt
    29641 cgatttgctc ggcagttttc ttctgttcct tgctcgaatt tgcttctgct tcttgcagtt
    29701 tttgaatctc ctcctcactt aaaaagggaa taagcttcat cgattcaact cctctcttat
    29761 tttttcaagc caagcttgct tgagtttttc tccgaccaga cgcttgctat cagctaggtc
    29821 caactttttt aggaaacggg cttggcccag atggtaattg gcttcaaagc ctgtaatagc
    29881 ctgatgttgc tggacgtatg gggcaatgct tctgccattt tcagtataag gattgatggc
    29941 gaaccggcct gctaaaatct tctcagcagc tttcttgtaa agataggcat tgtagtccag
    30001 taggagctga aattcctcat ctgtcagttg attagccttg tttttgttat aaaattcgcc
    30061 taaataactg ctttcttttt ccaagaagag cccttggtat ttcatagatt tgctggcttc
    30121 taccactgct cctgcaagat tttttaccgc catcagagat tggacaggtt cagccatttc
    30181 caagtacatg gcgccgaaaa agttctgctc cccttctctt tttagggcag caagataggt
    30241 tggtaactga gaattgagcc cattaaagaa atgaggaaac tggaactgag tcagactgga
    30301 tttgtagtct actactccta tcgctccatt agctttcaaa cggtcaatcc ggtccacctt
    30361 gcctcgtaca aagacactgc gtccattgtc taattgaata aaggcttggt cttttccacc
    30421 aaaatttgct tcttctttga tggtttcgat ggctggattg tgtcggagaa tatgtccagt
    30481 cgtccgtgca acatcaagca aaacttcctt ggtaaactgg gcttccaaac tttcttgata
    30541 aatagcttca aattcgcgtt cttgactggt ttcttgaata gcttgttcta gacgttggtc
    30601 aaaggaatct tcattaggca actgtaaggc gcgttcaaag atacgatgca agaaattccc
    30661 gtgactacgg gcatcaggat gcaaacgtaa ttcctcctgc aagcctaaaa cgtagcgtag
    30721 gaaataactg tattcattgc gataaaactc tgtcaaaccc gacgtagaca ggtaaaactc
    30781 ctgtttggca ggatagagag cttgcaaggt gtccttggct aaggtcttgc tgcttggact
    30841 ggttgggata gctggatttt ccagaccttg ctgatctagt tttttaccta tgacacgcga
    30901 cagaaccttg acaaaagtca aatcttgctc agtatcgctc atctcaccct gctggtgata
    30961 ggcaaccaga ctagacaaaa gactgtgata ggaccccata tcctccttag acagtctttt
    31021 gtgattcatc ctcttctctc tccgcctaaa tccaaaatgg atcaactctt gaagataggc
    31081 agattcctta ctttcacttt cgttaaaaag gcttggagcc gacaagaaca actgcttacg
    31141 agcagaattg accaaggaaa gcatagtgta gcgatttttc ttgagatttt cactgctggc
    31201 aatcagtaat tgaacgcctt cttcggtcgc ttggtttagg ttttgccttt cttcatctgt
    31261 cagaagactg gtgttttgag aaatttttgg taaattgtcc tgagttagtc caatagcata
    31321 gacaaagtca gcagtcaatg gtgcaatcaa atcgtaactc tgcaccagaa cagtgtccac
    31381 tgttgctgga atggtacggt attgggacaa actcattcca gaatggagca aggctaggaa
    31441 gtcttccaga ctaacctgtg aaccagcaaa aacagtcgca aattgttcta aaacatggca
    31501 gaaagccttc caaacttcgg tttgtctttc ctgttctaca gcttccaaag tggttgtcaa
    31561 atcttgtaac tgcttagtca cagctccttc ttttagaaag acactccatt tttgtaggag
    31621 gttttcagcc ttttgttttc ggctggcaaa gagggtttca agaggtgcta aaattctcag
    31681 gcggaggaca ttcaaacgct caagattaaa ttttccatgg tgggatttgg tgaaggtttg
    31741 ctgaaaggct ggcaagccat tgataccaag atagcggata tattgctcaa aagcatcaat
    31801 atcagactga ctgaggtcag tatacaaatc agttctaaga agattaatca aatcctcctg
    31861 acgaaaacgg taacgtttta aagctaaaat agactcgaca aactgagtca agggatgatg
    31921 agccatggct tcgcttctac caagataaaa aggaatctga tactggtcaa aaatagtttt
    31981 gagagataac tggtaagaag ctacatcccc caagagaata cgaaaatgct tgtagctcag
    32041 gtctgagttc tcatgtaatt tctgacgaat actacgggct actagctcca actcctcctt
    32101 ttgcgtcaaa caagaccaga tttgtaaatt ttcacggtct ttctcatcga catccaaagc
    32161 gagttctgaa aagtcataag aagactccaa caaacgagag accttgtcaa aactatccat
    32221 cttctcatga gtttgagaac agtcctgagc aggcgtttgg tatttagaag ccagatgatg
    32281 gagaaatttt acgctggctt ggtagagatt gccctcgcta aaaggactgg tataggcttt
    32341 cttactagca taagccccga taacaatctc aacacctttg ccgtgaagta agtccacaac
    32401 ccgctcttcc tcagcagaaa aacgagtaaa gccgtcaatg accaaggtga tttgattaaa
    32461 atcactactt accttgtcat tctcaatagc ctcaatcaaa tgggacaact gacttccctg
    32521 ggctaactga ccttgattaa gataggctgt tactttctca aaaatcaaga gtaaatccgt
    32581 cctcttatcc tcatctgtta aattctccaa gtccaaaaaa ctcatctgag atttggtcat
    32641 ctcatggtaa agctcaatta actgctggat caattgagga tcctgcttaa tagcgccata
    32701 aacacgcaag tccttgggat cgagttcggc aaggcatttg taaaaggcca acccaagacc
    32761 gatatcatca agagtagttt taactggtaa atcattcaag accagataac gagccatttg
    32821 agcaaagcgc gtgacggtaa tcgaaaaaga agcctgctgg gacaagcatt ccagcacggc
    32881 gcgttccttt tcaaaagaaa gagagttggg ggcaatgtag aagacccgct tgccagctgc
    32941 aactagctct tctgcctctc ttgttagaat ttctgtcaaa gaagttcgaa tatcagtata
    33001 aagtaatttc atctcagcct cgttggaatt tttcatcacc ctatattata ccatgattag
    33061 cctcgtaaat ctgttaaaat atttaggcca tcctttcttt tcttcatcat ctgctaaatc
    33121 ttaaatactt agctttactt gtattagata gaataagtct ggccactgaa aatcatataa
    33181 taaaaaagcc tcggtaacaa ggctttgagt tttatgattg tttcttaggt acggaataca
    33241 cttcaatgtg ttgtcccagt gtcttaatgt cgacgggtag attgtctgat ttattgccat
    33301 caacatcgga ctctaattcg atatcagaag aagttttaat attacgtgcc tttatatatt
    33361 caatattctt gatagaatga ttgaactata gtaaattgaa actataatag tacaccgtgg
    33421 atgctaaaat atttctagaa attaatttga tttccctaat caagctgttc gtatcttatt
    33481 tcaatctact attttcaatt gactatataa attgtctaag cgaaacaacc gataagaatt
    33541 ggcacaaaag cgaccgtatt tttgttacca atacaggaaa accagttcat agttctatct
    33601 tgagcaagtc tctccagcga gcaaacgaac gccttaaaaa accaattccc aaacatctgt
    33661 cccctcacat cttcagacac accactatta gcatcttatc agaaaataaa attcctttaa
    33721 aaacaatcac ggacagggtt ggtcattccg actctgaagt cactacttcc atctacaccc
    33781 acgtcacaaa gaacatgaaa gatgaagcaa tcaatgtact ggataaagtt atgaaaaaga
    33841 ttttttaaaa agttttgccc cttttttgtc ctctaaatac aaaaatagcc cttcggataa
    33901 aatccgaggg gctagaaacg ttgttaaatc aacggccgaa cttttgaatt tcatggttcg
    33961 ggataaaata gttcactgaa ctattttatt ttttaaggtt gtagaatgat ttcaatccac
    34021 gatattcagc tacttcacca agttggtctt cgatacgaag caattggttg tatttagcga
    34081 tgcggtctgt acgtgaaagt gaaccagtct tgatttgtcc tgcgttagtt gcaactgcga
    34141 tatcagcgat tgttgaatct tcagtttcac ctgaacggtg tgatacaaca gcagtgtaac
    34201 cagcttcttt agccatttcg atagcttcaa aagtttcagt aagagtaccg atttggttaa
    34261 ctttgataag gattgagtta gcagcacctt cttggatacc acgtgcaagg tagtcagtgt
    34321 ttgttacgaa gaagtcgtca ccaacaagtt gtactttctt accaagacgt tcagtaagag
    34381 ctttccaacc atcccagtcg ttttcatcca taccatcttc aatagtgatg attgggtatt
    34441 tgttaaccaa ttcttcaagg tagtcgattt gttctgcaga tgtacgaaca gcagcacctt
    34501 caccttcaaa tttagtgtag tcgtaaactt tacgttcttt atcgtagaat tctgatgaag
    34561 cacagtcaaa tccaataaat acgtctttac ctggtacata tccagcagct tcaatcgcag
    34621 caaggatagt ttcaacacca tcttcagttc cttcgaaacg aggagcgaat ccaccttcgt
    34681 cacctacggc agtttccaaa ccacgtgatt taaggatttt cttaagagcg tggaagattt
    34741 cagcaccgta acgaagggct tctttaaatg ttggcgcacc aactggcaag atcatgaact
    34801 cttggaaagc gattggagcg tcagagtgag aaccaccgtt gatgatgttc atcattggag
    34861 ttggaagaac tttagtgttg aatccaccaa ggtagctgta aagtgggatt tcaaggtagt
    34921 cagcagcagc acgagctaca gcgatagaca caccgaggat tgcattcgca cccaatttac
    34981 ctttgttagg agtaccgtca agtgcgatca tagcacggtc aatagcttgt tgatcacgta
    35041 catcgtagcc aatgatagct tcagcaatga tgttgtttac gttgtcaaca gctttttgtg
    35101 taccaagacc accgtaacga gatttgtcac cgtcgcgaag ttcaactgct tcgtgttcac
    35161 cagtagaagc tcctgatgga accataccac gtccgaaagc acctgattca gtgtaaactt
    35221 ctacttcaag tgttgggtta ccgcgtgagt ctaggacttc gcgagcgtaa acatcagtaa
    35281 taattgacat tttttactct ccttatgagt taaatttttt acacctctat aataccttaa
    35341 aacccctcct ttttcaagaa aaaacgttat ctttgtgcaa cttttcctta actttataaa
    35401 gtaatcgctt tcttttgtct gttttattct aacttttatg atatactgtt ttcatgacag
    35461 atttatcaaa acaattactt gaaaaagctc atggtgggtt aaaaataaat ccggatgagc
    35521 aaagacgcta tcttggtact tttgaggaaa gagttcttgg atatgtagat attgacacag
    35581 caaatagccc tcagttagaa aaaggctttt tatttatttt agaaaacctt caggaaaaag
    35641 cagagccact atttgtgaag atttcaccaa ctatcgaatt tgataagcaa gttttctact
    35701 taaaagaagc aaaagaaact gatagtcaag ccaccatagt atctgaagag catattactt
    35761 ctccttttgg cctggttatt catagcaatg caccagttca agtagaagaa aaagaccttc
    35821 gacttgcttt tccaaaactt tgggaagtta aaaaggaaga accagccaaa acatccttat
    35881 ggaagaaatg gtttagctaa atcttgcaca tatttaataa gtgcccaata ttggcagccg
    35941 tgcgctccag atagagactg gcatttttca aactatcttc taaaggttca cttttctcca
    36001 aaatagaaaa gacagcttgg atattttcaa atggtagggg aggtaaatct tcagcaagac
    36061 taccgcaaat agcaataaca ggaactccaa caggggttct ttttgcaaca cctataggcg
    36121 ctttcccagc aaagctttga ctatcaagtc ttccttctcc aacaacaacc aagtcagcat
    36181 ctgaaacttt cttatcaaag ttgattaagt ccaagcaggt atcaattcca gacacgatac
    36241 ttgcctgagc aaaggcacac aaaccaccag caaggcctcc acctgctcct gctcctttaa
    36301 tttctaatgt tgcaggtgag aatttttcat aaaaatcttg gatcgcctga tctacgactg
    36361 caaacatagt cggatgtaga cctttttgat tgccaaaagt gtaagtcgca ccttgatgac
    36421 cacataaggg actcacgaca tctgctagaa tatgaatttg aacaccttca ggaattttat
    36481 agcaattttc tgttgaaaca gaagctaagt ctaataagga ttgaccggaa gcaggcaaga
    36541 catttccatc cctatcataa aattgataac ctaaaccagc agcaatcccc agtcctccat
    36601 cattactggc cgtgccacca acaccgatat aaatatcttt aatcccttta gaaatgagat
    36661 ggagaatcaa ctctccaata ccataagttt ggatttgaag tggatttcgt ttctctagcg
    36721 gaatttttcc aagaccaacc aagtcagcta cttcaaatag tgccagttcc cctttttgaa
    36781 aatagcgcat ggcttctttt tgtccaaaag ggtctgtcat tttaggtcaa gagaatgtcg
    36841 gatagcatct acagtacctt ctcccccatc accaacaggg cagaggagat attctacatc
    36901 tgctatcgat tgttggaagc ctctttttat tgcttcagct acctgttgag ctgtcaaact
    36961 ttccttaaac gaatctggtg caattacaat cttcatattt tccctcattc taaacagtca
    37021 atcaaaggaa gaacttctaa aaaatccctc ttgtcaacat gatgtggtat ttcttttttg
    37081 agcacttctt tggcacaaaa ggcgattcct aactccgccg acttcaacat taatagatta
    37141 ttgaccccat caccgattgc caccgttctt tctttagaaa gttttagttt ctttctccat
    37201 ttttccagag tctctttttt gacctgggga cttataattt gttcaactaa ttttcctgtt
    37261 aaaagacctt ctttgacttc aagctggttg gcagtgaaat aggcaatacc aagggatttt
    37321 gctaatctct ccaactattg gtatccacca gacaccacac accagaccaa ctaggatgcc
    37381 attcttttgg agaatagaga tgaactctgg gacatttagc gatagatgaa ttgagttgaa
    37441 gacgttatca aagaccaaaa taggaagacc ttccaacaag gacactcttt ttcttaaact
    37501 gctttcaaag accaactctc ctcgcattgc ccgacttgta atctgcgaaa tttccgcctc
    37561 atgacctgcc tctctcccta aaagatcaat cacttcttct aggattaagg ttccatctac
    37621 atccaaaaca cacaagcctt ttacttgaga catcagttct cctctctaaa cagcctaaaa
    37681 atcgtatgaa gtcatcatac gattttatct attaattaac taaactatgg tacaagtcaa
    37741 ggtatgactt gcaggctgta tcccatgaga agtcactctc catagcttgt ttttgtaggt
    37801 ttctccaaat gtctggatgg tttctataca agtccaatgc tgtttggaaa gtccaattta
    37861 accaataagg agatagattg tcaaagctaa agccagtacc gcttccttcg attggattga
    37921 aagcgcgaac tgtatctcgc aagcctccaa cttcatggac caatggcaag gttccataac
    37981 gcatagccat catttgagac aagccacacg gttcaaaacg acttggcatg aggaagaggt
    38041 cacaagcagc gtagatttcc tgagcaagtt tgacatcaaa agtgatattt gttgatagct
    38101 tgtctgggta aatctgagca aaccatgaga aagctccttc aaaggctgga tcgccagttc
    38161 ccaaaagaac aatctgaaca tcttcttgca agatatggtg aagactttcg accaccacat
    38221 caaaaccttt ttgacgtgtc aaacgagaaa caattcccac cagtggaacg tctgctctaa
    38281 caggcaagcc aactctttct tgcaattttg ccttattttt ggctttccca gacaaatctt
    38341 cctgattgaa atgatagtct aaaagagcat ccgtctgagg attataaaga tcagcatcaa
    38401 tcccattcac gataccagat actttaccag actccatttt aagaatctga tccaaattac
    38461 atccaaactg actagtcata atttcatgag catagctagg tgaaacggtt gaaacacggt
    38521 tcgcatagag aatacctgcc ttcatccagt tcagacagtt gttccatcga agggtgccat
    38581 cagcgtaacg ttcaaagcca actccaaaca aatcacccaa cattccttct gaaaattgtc
    38641 cttggaattc taaattatga atggttaaaa ctgtttcaat gtcctcatag gcttgaatcc
    38701 aacggtattt ttccttcaac aagaaaggaa tcatagctgt atggtagtca tgaacatgga
    38761 gaagatcagg aataaagtca atcctttcca tagcctcaat ggcagccagt tggaaaaagg
    38821 caaagcgttc tccgtcatca aaatcaccgt aaacatgacc acggaagaaa taatattgat
    38881 tgtcaataaa gtagaaggtt acaccattta atactgtttt cttaattcca caatactgtc
    38941 tgcgccaacc aacgctcacc tcaaaatgaa gtacatcttc aatctgattt ccaaatttag
    39001 cctctaccat atcatagtag ggtaaaatca ctgcaacttc gtgcccagct tttaccagtg
    39061 attttggaag agcgccaatg acgtctccca aaccacctgt ttttgaaaag ggtgcaccct
    39121 ctgctgctac aaataaaatt ttcatgaatg aatatcctct gttactttag cacctttctt
    39181 aaccacaact ggatgttctg cagttcctcg aatcacaaca ccatgctcaa cttcaacccc
    39241 tttgtccaag atagcatatt cgacctgagc cccttctcca ataacaacac gagggaagag
    39301 caggctatct ttaaccaagc tatccttatg gacatgaata ttacgtgata gaacagaatt
    39361 agctacttga ccttcaataa tactaccaga ggcaaactga gaagtgctta ccttagatgt
    39421 attagcatag taagttggct cttcgttttt gacctttgta taaatctttt ggtttggtga
    39481 gaaaagagaa tagaattttt gtgattcaag catatcgata ttcgcttgat aataagattt
    39541 aacagagtga atattggcta gatagcccgt gtactcgtag gcgaaagctc cctcttttac
    39601 agccaaatcc cgtaaaacat agcgaaattt ctctggatgt tcttttttag cttcttcttc
    39661 caagtgttca atcaaccaag gtgtatcaac gacaaagata tctgtagaca tattgaacgt
    39721 ttcagctgtt gacttgctat caaagagttt atgagaaaga acatggtctg tttcatctac
    39781 atccaagatt gcatttactt ctgaaatatc tttcttagct agttttttat aaactacagt
    39841 gataggctct tttgttgtac tatgtaggtg gaaaacttgg ttcaaatcaa tgttaataag
    39901 aacatcgcag ttgagggcaa ccgtttggtt tgagccagaa cgtttcaaat aagtaagaag
    39961 ctgttggtag tattcttttc caactgtact actttctaca cgggtattgt aaattcctag
    40021 atagtaatga ctaagaaggg ttgataagcc ccactcgcgt cctgaacgaa tatggtcaaa
    40081 tactgagctg atattatcct gctggaaaat accaaagaca ctacgaacac ctgcattagc
    40141 aaggcttgaa agtgggaagt caatcaaacg atatttccca ccaaatggca aacttgctac
    40201 tggacgatgg tctgtcaatg tcgacatatt gtgaaaacca actgtatttc ctaaaatggc
    40261 agaatattta tcaatcttca tctgttgcta cccccactac ttcattatat cctacaactt
    40321 gtacttcatc tgttccatca atttcgacac cgtcagaaat aatcgcacct tcaccaataa
    40381 tggcacgttt aatcttagct ccttgaccaa tgatagctcc actcatgata actgaatcaa
    40441 ggacttccgc tccttcgcga acttgcgcgc ctgttgaaag gatagaatgt ttaacagttc
    40501 catcaacgaa acatccgtct acaactaatg agtcttccac atgagcattt gccccgagga
    40561 agtttggtgg tgaaatcaag tttcttgagt aaatcttcca ttgacggtta cgactatcca
    40621 aggcattttc tggagaaata tactccatgt tcgcttccca aagtgactca atagtaccaa
    40681 catctttcca ataaccacta aattcgtaag cataaacact ttcacctgac tcaaggtaat
    40741 ttggaatgac atttttacca aagtctgaca tgccaacctt gctcttttca gcagcgacta
    40801 acatattacg aaggcgttgc caatcaaaaa tgtagattcc catagaagct tttgtagatt
    40861 taggttgagc tggtttttct tcaaattcaa caatacgatt gttagcatct gtgttcatga
    40921 taccaaaacg gcttgcttct ttaagaggga cgtctaaaac tgctactgtc aagctggcat
    40981 tattatcctt atgagactgg agcatatcat catagtccat tttgtagatg tggtccccag
    41041 acaaaatcaa gacatactca ggattgacac tgtcgatata gtcgatattt tggtaaatag
    41101 cgtgactagt tccctcaaac caacgatttc cttcacttgc agaataaggt tgaagaatag
    41161 agacgcctga attaataccg tctagtcccc agcttgaacc attcccaata tggttgttga
    41221 gagcaagtgg ttgatactgt gtaacgaccc caacattatg aatccctgag ttggcacagt
    41281 ttgatagggc aaagtcaatg atacggtagc gcccaccaaa ttgcacagct ggttttgcga
    41341 tgctttgagt gagtttaccg agacgagttc cttgcccacc agcaagaatc aaagctaaca
    41401 tttcattttt cattttctac tcctttttgg tttttatttg tgacggtttt agtagatttc
    41461 aagcgacgtt tgattttcca tacacttgct cccatagccg gtagagtaaa ggttaaggtc
    41521 tgctcataat ctttccatag gccttcttgc gtttgaacag tttgattatg ttctttccaa
    41581 acgcctcccc actcttccaa ctcagtattc catacttctt cgtaaattcc tgcaacgggt
    41641 agtccgattg taaaatcttt ccgctcaaca ggtaccatgt taaagataca gactaacatt
    41701 tctccctttt tacccttacg aataaaggaa agaacactct ggtctcgatt atccgcatca
    41761 atgatttcaa taccatcata gctggtatcg atttcccaca gacagcgatg atctttgtaa
    41821 aactggttta gctgagaagt gaaatacttc atcttagcat tcattgggtc ttctaggtta
    41881 gaccattcca actgttcttc agatttccat tctaggaatt gaccgtattc gctacccatg
    41941 aagagcaatt tcttaccagg gtgacaaatt tggtacgtat agagattgcg caagcttgcg
    42001 aattgattgt aacgatctcc ccacatctta tgcatcatac tcttcttgcc atgaaccact
    42061 tcatcgtgcg agaatggcaa gagataattc tccttgaaaa catacataaa gctgaaagtc
    42121 accaggttaa agtcatattt acgataaatc ggatcttctt cgtagaaacg gaggatatca
    42181 ttcatccagc ccatgttcca tttgtagtca aatcctagac caccaatctc tttcattccc
    42241 gtaatcttgg tcgcagacga actttcttct gcaatcatca tcacatctgg atattctaac
    42301 ttaataactt cattcaagcg ttgaagaaaa taataacctt catagttgag atttccgcca
    42361 tctttattag gtgtccatgg agcattatca tagtccaaat agagcatgtt gctaacagca
    42421 tccacacgaa taccatccaa atgatagaca tcaatccaat gcttaatgca agaaattaag
    42481 aaggactgga cttcattttt tccaaggtca aaattaaggg caccccaacc atggttatga
    42541 gccttattat ggtcttggta ttcaaaagtc ggtgtcccat cataataggc taaggcatca
    42601 tcgttgatgg taaagtgacc tggtacccag tccacaataa ccccaatatt atgggtatga
    42661 cactcctcga caaaatcttg aaactcctct ggtctgccat aagcatgctc taaagcgaag
    42721 taacccataa gctgataccc ccaactcaag cccaaaggat gggacatcaa gggcataaac
    42781 tcaatatgag tatagttcat ttcaacgaga taaggaatga gttcatcctt gagctgggca
    42841 aaactataag gactgccatc agaatttctt ttccatgatc cagcgtgaac ttcataaata
    42901 ttgacaggac gctcttcaaa gccccaacgt tttcttcgtg ccagccaaag tccatccttc
    42961 catttcttct caggaagctc tgttaagatt gcccctgttc ctggacgagc ctcatacctg
    43021 acagcaaaag ggtcaatctt catcagttga tgaccatttt gacgtgtgac atgatatttg
    43081 taaatatgcc cttcttgagc catattggta aagacttccc agaccccaaa atcatttctt
    43141 accattggaa tctgattttc aatccagttg gtaaaatcac caaccaagtg aacagcctga
    43201 gcattaggtg cccaaacacg gaaggtatag ccatgctctc catttagttc ttccctatgt
    43261 gctcctagat aatgttggag ataaaaattt tcacccgtca taaaggtttt taatgcttct
    43321 ctattatcca tatactcccc ttctcctgta agcgttttct atgtttttat tatactacct
    43381 ttttagagaa gattcaagta aattactata cttctttaat tattttgaaa atctacaaca
    43441 agttcactta ctcgttcaat tgtaaatcaa tattttttca aaaaattgcg aaaacgcctt
    43501 tctttttcta
//
LOCUS       DBEROU010000012        43053 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.012, whole genome shotgun sequence.
ACCESSION   DBEROU010000012 DBEROU010000000
VERSION     DBEROU010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 43053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43053
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.012"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            22..95
                     /locus_tag="ACL8CL_000710"
     tRNA            22..95
                     /locus_tag="ACL8CL_000710"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56..58,aa:Ile,seq:gat)
     gene            102..189
                     /locus_tag="ACL8CL_000711"
     tRNA            102..189
                     /locus_tag="ACL8CL_000711"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:136..138,aa:Ser,seq:gct)
     gene            complement(315..1250)
                     /gene="birA"
                     /locus_tag="ACL8CL_000712"
     CDS             complement(315..1250)
                     /gene="birA"
                     /locus_tag="ACL8CL_000712"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HGS1329999.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTAIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(1247..2107)
                     /locus_tag="ACL8CL_000713"
     CDS             complement(1247..2107)
                     /locus_tag="ACL8CL_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS1330000.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKAPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            2215..4377
                     /locus_tag="ACL8CL_000714"
     CDS             2215..4377
                     /locus_tag="ACL8CL_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HGS1330001.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHSVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATIASYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIVTLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKVGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPDYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHEIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            4469..5728
                     /locus_tag="ACL8CL_000715"
     CDS             4469..5728
                     /locus_tag="ACL8CL_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS1330002.1"
                     /translation="MEWYKKIGLLATTGLALFGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEEITRDFEKENPKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            5742..6608
                     /locus_tag="ACL8CL_000716"
     CDS             5742..6608
                     /locus_tag="ACL8CL_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1330003.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAAFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSMVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFLLIVVIS
                     IIQLRVSKKFEI"
     gene            6622..7455
                     /locus_tag="ACL8CL_000717"
     CDS             6622..7455
                     /locus_tag="ACL8CL_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1330004.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAIMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIISGMSNGAV
                     K"
     gene            7766..9208
                     /gene="gtfA"
                     /locus_tag="ACL8CL_000718"
     CDS             7766..9208
                     /gene="gtfA"
                     /locus_tag="ACL8CL_000718"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HGS1330005.1"
                     /translation="MPIQNKTMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKDLFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYALNEFYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRPVVKALLNLFSFRNRSEAFDLEGTTEIETPTAHSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            9346..10539
                     /locus_tag="ACL8CL_000719"
     CDS             9346..10539
                     /locus_tag="ACL8CL_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008754 - O antigen ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HGS1330006.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            11224..13203
                     /locus_tag="ACL8CL_000720"
     CDS             11224..13203
                     /locus_tag="ACL8CL_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1330007.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KAVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            13270..14766
                     /locus_tag="ACL8CL_000721"
     CDS             13270..14766
                     /locus_tag="ACL8CL_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1330008.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSIALPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            14766..15692
                     /gene="oppC"
                     /locus_tag="ACL8CL_000722"
     CDS             14766..15692
                     /gene="oppC"
                     /locus_tag="ACL8CL_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HGS1330009.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSIRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            15701..16768
                     /locus_tag="ACL8CL_000723"
     CDS             15701..16768
                     /locus_tag="ACL8CL_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330010.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIVQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPRHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            16779..17705
                     /locus_tag="ACL8CL_000724"
     CDS             16779..17705
                     /locus_tag="ACL8CL_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1330011.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQNIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            18195..19133
                     /locus_tag="ACL8CL_000725"
                     /pseudo
     CDS             18195..19133
                     /locus_tag="ACL8CL_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512061.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate binding protein"
     gene            19145..20034
                     /locus_tag="ACL8CL_000726"
                     /pseudo
     CDS             19145..20034
                     /locus_tag="ACL8CL_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            20047..20850
                     /locus_tag="ACL8CL_000727"
     CDS             20047..20850
                     /locus_tag="ACL8CL_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330012.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSMDEGDLLLAGKSIKNLSVRKRAKDIARVFQAPKMGTASRLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGGQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQLTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLR"
     gene            complement(20882..21592)
                     /gene="treR"
                     /locus_tag="ACL8CL_000728"
     CDS             complement(20882..21592)
                     /gene="treR"
                     /locus_tag="ACL8CL_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HGS1330013.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            21777..23744
                     /gene="treP"
                     /locus_tag="ACL8CL_000729"
     CDS             21777..23744
                     /gene="treP"
                     /locus_tag="ACL8CL_000729"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HGS1330014.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIAA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVNHEPVELTSVEII
                     SPLTGQVKELSQATDPIFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATIPGTYPTTIQAGASLMVATRI"
     gene            23922..25547
                     /gene="treC"
                     /locus_tag="ACL8CL_000730"
     CDS             23922..25547
                     /gene="treC"
                     /locus_tag="ACL8CL_000730"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HGS1330015.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNSNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDTSENAGFSTGTPWLKVGKSYKDINVENEIKGLIFTFY
                     QDLIRLRKEMPIISEGNYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            25803..26051
                     /locus_tag="ACL8CL_000731"
     CDS             25803..26051
                     /locus_tag="ACL8CL_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HGS1330016.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            26258..27052
                     /gene="racE"
                     /locus_tag="ACL8CL_000732"
     CDS             26258..27052
                     /gene="racE"
                     /locus_tag="ACL8CL_000732"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HGS1330017.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            27049..28020
                     /locus_tag="ACL8CL_000733"
     CDS             27049..28020
                     /locus_tag="ACL8CL_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HGS1330018.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            27996..28517
                     /locus_tag="ACL8CL_000734"
     CDS             27996..28517
                     /locus_tag="ACL8CL_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HGS1330019.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWMEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            28514..28975
                     /gene="cbpB"
                     /locus_tag="ACL8CL_000735"
     CDS             28514..28975
                     /gene="cbpB"
                     /locus_tag="ACL8CL_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0180001 - cyclic-di-AMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP-binding protein CbpB"
                     /protein_id="HGS1330020.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            28972..29706
                     /gene="xerD"
                     /locus_tag="ACL8CL_000736"
     CDS             28972..29706
                     /gene="xerD"
                     /locus_tag="ACL8CL_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HGS1330021.1"
                     /translation="MRDRISAFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETSLK
                     IYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEILYL
                     DSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVTIP
                     TALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFILRQ
                     IENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            29706..30434
                     /locus_tag="ACL8CL_000737"
     CDS             29706..30434
                     /locus_tag="ACL8CL_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HGS1330022.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLIGRDQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            30453..31022
                     /gene="scpB"
                     /locus_tag="ACL8CL_000738"
     CDS             30453..31022
                     /gene="scpB"
                     /locus_tag="ACL8CL_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HGS1330023.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            31009..31731
                     /locus_tag="ACL8CL_000739"
     CDS             31009..31731
                     /locus_tag="ACL8CL_000739"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HGS1330024.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            31731..31973
                     /gene="yidD"
                     /locus_tag="ACL8CL_000740"
     CDS             31731..31973
                     /gene="yidD"
                     /locus_tag="ACL8CL_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HGS1330025.1"
                     /translation="MKRILIALVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPIPDHFSLKRNQEGE"
     gene            complement(32198..33163)
                     /locus_tag="ACL8CL_000741"
     CDS             complement(32198..33163)
                     /locus_tag="ACL8CL_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1330026.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETSTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(33259..34011)
                     /locus_tag="ACL8CL_000742"
     CDS             complement(33259..34011)
                     /locus_tag="ACL8CL_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330027.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINNKKICIYS"
     gene            complement(34008..34964)
                     /locus_tag="ACL8CL_000743"
     CDS             complement(34008..34964)
                     /locus_tag="ACL8CL_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HGS1330028.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSLFFLALQGYLKTLMKQDLVFVLLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFSFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(34954..35913)
                     /locus_tag="ACL8CL_000744"
     CDS             complement(34954..35913)
                     /locus_tag="ACL8CL_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1330029.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGDLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(36210..36320)
                     /locus_tag="ACL8CL_000745"
     CDS             complement(36210..36320)
                     /locus_tag="ACL8CL_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase"
                     /protein_id="HGS1330030.1"
                     /translation="MNYLFQEDSSSQIFNLGTKKGYTIKEIFKTAEELLN"
     gene            complement(36336..37037)
                     /locus_tag="ACL8CL_000746"
     CDS             complement(36336..37037)
                     /locus_tag="ACL8CL_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS1330031.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SHASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTTNVAGMNQV
                     LKVLSEVGIQKIMFSSTASLCGNNCIDKPVTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
     gene            37438..38502
                     /gene="pepA"
                     /locus_tag="ACL8CL_000747"
     CDS             37438..38502
                     /gene="pepA"
                     /locus_tag="ACL8CL_000747"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HGS1330032.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKAEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
     gene            38524..38820
                     /locus_tag="ACL8CL_000748"
     CDS             38524..38820
                     /locus_tag="ACL8CL_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGS1330033.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETTKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            39502..39945
                     /locus_tag="ACL8CL_000749"
     CDS             39502..39945
                     /locus_tag="ACL8CL_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGS1330034.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            39949..40713
                     /locus_tag="ACL8CL_000750"
     CDS             39949..40713
                     /locus_tag="ACL8CL_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HGS1330035.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERHPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHDIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAQYDIFRLENGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            40728..41456
                     /locus_tag="ACL8CL_000751"
     CDS             40728..41456
                     /locus_tag="ACL8CL_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1330036.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKIFLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVA
                     NPETILKAPATDFVADLFGGSVHD"
     gene            41449..42969
                     /locus_tag="ACL8CL_000752"
     CDS             41449..42969
                     /locus_tag="ACL8CL_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HGS1330037.1"
                     /translation="MTNLIATFQDRFSDWLTALSQHLQLSLLTLLLAILLAIPLAVFL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENVIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
BASE COUNT        12477 a         7982 c         9666 g        12928 t
ORIGIN      
        1 aattaccaag ttttttaact tgggcgcgta gctcaggtgg ttagagcgca cgcctgataa
       61 gcgtgaggtc ggtggttcga gtccactcgt gcccattaat tggagaatta ctcaagaggc
      121 tgaagaggac ggtttgctaa atcgttaggt cgggtaactg gcgcaagggt tcgaatccct
      181 tattctccgt tttaatgaga gtttgtaact ctctttttta tttttagagc caataaaact
      241 tcagatccgt aaaacttaat tgaattacta atctgaagtt atttttatat cgtttatatt
      301 ataattgtgt tattttactt ccaactattg agagaaattt cgccactatt tagccagatt
      361 tcttttccgt tatcacattg aactaaaagt tttccatttt ctgagatgtc tttagcaagt
      421 cccttgtagt ctttttgctc tagtgtgaaa gtgacttctt ttcctagaat gaatgactgt
      481 tttttgtata ggtataatag ctcttctgct ggtgtttcga agaaagcacg ccagatttct
      541 atgatcaatt cattccttgt tataggagct gtagctttaa ataagctggc agctttttct
      601 tttaattcct gagggaagtc tttaatagtg aaattgatac ctactccaat aatgatatct
      661 gtgactaagc cagtttctac agaggtcatt gcttcagtaa ggattcctcc aattttatga
      721 ttgtttagat agatatcatt gacccatttt atgtcgacat ctattaaagt taggttctta
      781 atggctttgt agacagctcc agctacaagt agtgtgtagg atggtaattt gtcatagggg
      841 agatttggtt taagatggag tgtcatataa ataccacctt gtggtgagta gaaggaacgt
      901 tgaaaacggc ctcggcctgc tgtttgatag gaagctagat agagggtatt tgcttcatgg
      961 cctaaatcaa ttgcttcttt tgcatctagt tgtgttgatt ttgtttcggg tttaaagctg
     1021 actttaattg gaagattttc ttctagaatc tctggaagaa taaggtcacc attcatcagt
     1081 ttatatcctc tatttttgat actatcaatt tcaatgcctt cttgttctag tcgcttgatg
     1141 gctttccaaa ttgctgttcg gcttagggat agtttttctg cgattttttc tccgctgata
     1201 tagtcggttt ctttagatag gatttggtag acagcttggt aggatttcat aatgttgcct
     1261 ttcttactag ttggtattga gagtattctt ttcttgtatg acttggagtc tgattaaagt
     1321 attgtttata agctttcgaa aaatggagtg gatctgaaaa acctaccgag tatgcaatta
     1381 ccttgatgga ctcttgggta ttttcgagaa gttgtctagc tcggtgcatt cgaacgtaga
     1441 gtaggtattc tttgggtgat aaggtattaa attctttgaa tacgcttgat aagtagcttc
     1501 tgtgaacgga tagttctttt gctaaatctt gaattgtaag tgattgagga tagtggctat
     1561 caattaatcg tttgcattca agatagagtt ggtgggttga tgaaatattc ttttttttct
     1621 gattgggagc aatagttccc agatgaaaca tcagttcatg aagttgtccc atgatatgga
     1681 gttgagctaa ttcacttgat tttgtaatct gagcgaagcg gacaatgtct gagatgagtt
     1741 ttgcagtagt ctgggtatga caagtttcag attggatgag ataggattga tcagaaattt
     1801 gggaaagagc aaaataatca ggggctttcc ctccagtgat tcctaaccag tagtaggccc
     1861 aaggttcttt actatctgct tgataaaagg ttagttcctc tggttttaat agaaagaaat
     1921 ctccttcttt taaatcaaca attttaccct tgtaatgaaa ttttccttgt cctttagtaa
     1981 tgtaatgtag aacgtatgta tcacgaatgg ctggaccaaa agagtaatta ggtgtgcatt
     2041 cctcatatcc ataaaagctt agggcaaggt cgattgttcc agtctggtat tctgaaaaaa
     2101 ctaacatgtg ctacctccta cctaacattt taccatattc ttgagacatt tttctatttt
     2161 ggaaagcgat ttcaggtgat aaaatataat caataaagtg atgaggtgaa gtaaatggga
     2221 gttaggatag agaataatct attttatgtt gagagtaaaa atctaagttt gattattgaa
     2281 aatcgaaatg gctacttact tttgaaacat ttaggaaaga ctattaagaa ctataaaggt
     2341 tccaatagtg tttatgaacg agaccatgcc ttttcaggaa atccaacggc tactaatcga
     2401 acctttagtt tagatactca gcgacagatt tttggacaac atggcttagg agattttagg
     2461 aaaccaacca tacaggttca gcatagtgta actgaagtaa cagactttcg atttgtagaa
     2521 gcaaagattt taaaaggtca gaatggtcca cagggcttac cttctccaca tagcatggac
     2581 gatacagaga ctcttgtctt aatgttagaa gattctaagg ctcaacttag tctgactttg
     2641 tattatacta cttttaataa tgatgcgact attgctagct acagtaaatt agataataat
     2701 agtaatcagg aagttgtcat ccataaagat ttttctttta tggctgattt tccagctgca
     2761 gattacgaaa tagtaactct gcagggtgct tatgctcgtg aaaagactgt tagacgtcaa
     2821 caggtagaac aaggaatctt ttcgattagc tcaaaccgag gtgcttctgg tcatgctcaa
     2881 acaccagctc ttctactatg cgaacaagga gtcacagagg atgctgggaa tgtgtttgct
     2941 attcaactaa tgtatagtgg caactttgaa gcttttgttc aaaaaaatca attgaatgaa
     3001 gttcgggtgg ctattggcat taatccagaa aacttttctt ggaagttagc ccctgaggaa
     3061 tactttgaaa caccggtagc tttagtgact cattcagatc agggattaac tggtattagt
     3121 catgaaagtc agaattttgt actgaagcac attatgctaa gtgaattttc taaaaaagaa
     3181 cgtccaattc taatcaataa ctgggaagct acttactttg actttcagag agaaaaactg
     3241 ttagagttag cagatgaagc taagaaagtt ggcattgaac tttttgtatt agatgatggt
     3301 tggtttggca atcgttttga tgataatcgt gctttaggtg attgggttgt taatgaggaa
     3361 aaactgggtg gaagtctaga aagtctgatt tcagctatcc atgaaagagg tttgcagttt
     3421 ggactttggt tagaacccga aatgatttct gtagatagtg atttgtatcg tcaacatcct
     3481 gactgggcta ttcaggttcc tgattatgag catacttatt ctcggaatca attagtactt
     3541 aatcttgcca atcctcaggt agtagaatac ttgaaaagtg tcttagatca actcctatct
     3601 tatcatgaga ttgattacat taaatgggat atgaaccgca atatcactaa gctagggaat
     3661 ggattaactt atctagagac acagatgcaa tctcatcagt acatgctggg actttacgaa
     3721 ctcgtttctt atctgacaga gaagcacagc catattctct ttgagtcctg ctctggtggt
     3781 ggtggacgaa atgatcttgg tatgatacgc tatttcccac aagtctgggc tagtgataat
     3841 actgatgcca ttgcacgttt accaattcaa tacggttcat cctatctcta tccaaccatt
     3901 tctatggggg ctcatgtgtc agcagtaccg aatcatcaga tgggacgaat gacaccatta
     3961 gaaacacgtg gccatgtagc aatgatggga aatttgggct atgagcttga tttgacaaat
     4021 ttatcagatg aagagaaagc tacgattgct aatcaggtga acttgtataa agaattacga
     4081 ccagtagttc agttaggaca gcagtataga ctaattaatc ctgatactgc atccaatgaa
     4141 gcagctgtac aatttaatta cggaaatcaa acgattgtaa cctacgttcg cgttttatct
     4201 gttgtagaga ccatggaaac aactttaaaa ttaaaagatt tggatgaaga gggactatat
     4261 aaattacagg aaaatggcga agtttactca ggtgcagaac taatgtatgc gggcttaact
     4321 gttatcttat cccaaggaga ttttttgagt agacagtata tttttagaaa actatgatat
     4381 gtactgaccc caaaagttag atttattctg tctaactttt ggggtgcagt tcaataaggg
     4441 tgtataaatt tataaaggag gctttctaat ggaatggtat aaaaaaatcg gacttcttgc
     4501 aactacaggt ttagctttgt ttgggctcgg cgcttgctcc aactatggta aatctgcgga
     4561 tggcacagtg accatcgagt atttcaacca gaaaaaagaa atgaccaaaa ccttggaaga
     4621 aatcactcgt gattttgaga aggaaaaccc taagatcaag gtcaaagtcg tcaatgtacc
     4681 aaatgctggt gaagtattga agacacgcgt tctcgcagga gatgtgcctg atgtggtcaa
     4741 tatttaccca cagtccatcg aactgcaaga atgggcaaaa gcaggtgttt ttgaagattt
     4801 gagcaacaaa gactacctga aacgcgtgaa aaatggctac gctgaaaaat atgctgtaaa
     4861 cgaaaaagtt tacaacgttc cttttacagc taatgcttat ggaatttact acaacaaaga
     4921 taaattcgaa gaactgggct tgaaggttcc tgaaacctgg gatgaatttg aacagttagt
     4981 caaagatatc gttgctaaag gacaaacacc atttggaatt gcaggtgcag atgcttggac
     5041 actcaatggt tacaatcaat tagcctttgc gacagcaaca ggtggaggaa aagaagcaaa
     5101 tcaatacctt cgttattctc aaccaaatgc cattaaattg tcggatccga ttatgaaaga
     5161 tgatatcaag gtcatggaca tccttcgcat caatggctct aagcaaaaga actgggaagg
     5221 tgctggctat accgatgtta tcggagcctt cgcacgtggg gatgtcctca tgacaccaaa
     5281 tgggtcttgg gcgatcacag cgattaatga acaaaaaccg aactttaaga ttgggacctt
     5341 catgattcca ggaaaagaaa aaggacaaag cttaaccgtt ggtgcgggag acttggcatg
     5401 gtctatctca gccaccacca aacatccaaa agaagccaat gcctttgtgg aatatatgac
     5461 ccgtccagaa gtcatgcaaa aatactacga tgtggacgga tctccaacag cgatcgaagg
     5521 ggtcaaacaa gcaggagaag attcaccgct tgctggtatg accgaatatg cctttacgga
     5581 tcgtcacttg gtctggttgc aacaatactg gaccagtgaa gcagacttcc ataccttgac
     5641 catgaactat gtcttgaccg gtgataaaca aggcatggtc aatgatttga atgccttctt
     5701 taacccgatg aaagcggatg tggattagga gtagagagac tatgaaaaaa gtattacaaa
     5761 aatattgggc atgggctttt gtggtcatcc ccctcttgtt acaagcaatt ttcttctatg
     5821 tgccgatgtt tcaaggagcc ttttacagtt ttaccaactg gacaggattg acttataact
     5881 acaaatttgt cggcttaaac aactttaagc tcctcttcat ggatccaaaa ttcatgaatg
     5941 cgattggctt taccgcaatc attacgattg ccatggtggt tggtgagatt gcactcggga
     6001 tcttcattgc gcgtgtcttg aactctaaaa tcaaaggcca aaccttcttc cgtgcttggt
     6061 tcttcttccc agctgtttta tctggtttga cagtggcttt gatcttcaag caagtcttca
     6121 actacggtct tccagcgatt gggaatgccc ttcatattga atttctccaa accagtcttt
     6181 tagggactaa gtggggagca atctttgcgg ctgcctttgt ccttctttgg caaggggtgg
     6241 ctatgcccat catcatcttc ctagctggtt tgcaatctat tccaactgag attacagagg
     6301 cagcaaggat tgatggtgcg actagcaagc aagttttctg gaacattgaa ttgccttact
     6361 tgctaccaag tgtctctatg gtctttatcc tagccctaaa aggtgggctg actgcctttg
     6421 accaagtctt tgccatgacc ggtggtggtc caaacaatgc cacaacctca cttgggctct
     6481 tggtttataa ctatgccttt aaaaacaacc aattcggtta tgccaatgcc attgccgtaa
     6541 tcttgttcct cttaattgta gtgatttcga tcatccaatt gagagtatct aagaaatttg
     6601 aaatttaaga ggagaagcat gatgaaacaa gatgaaagaa aagccctgat tggcaaatac
     6661 attctattga ttctaggatc ggttctgatt ttagtgccgc tccttgctac cctctttagt
     6721 tccttcaaac ccactaagga tattgtagat aatttctttg gctttccaac caacttcaca
     6781 tgggacaact ttagccgtct cttagctgat gggattggag gctattattg gaactctgtc
     6841 gtcatcactg tcttgtcttt acttgcaata atgatcttta tccctatggc agcctactcc
     6901 atcgctcgca atatgagtaa aagaaaagcc tttaccatca tgtataccct cttaatcctc
     6961 ggaatcttcg tacctttcca agtcatcatg attccgatta cggttatgat gagtaaactc
     7021 ggtttggcta atacctttgg tttgatcttg ctctacttga cctatgcgat tccacagacc
     7081 ctctttctct atgttggcta tatcaaaatc tcgattccag aaagtctgga tgaagcagca
     7141 gagatcgatg gggctaatca atttacaacc tatttccgca tcatcttccc aatgatgaaa
     7201 ccgatgcatg cgacaaccat gatcatcaat gccctttggt tctggaatga cttcatgttg
     7261 ccactccttg tcttgaaccg ggattccaaa atgtggactc tgcctttgtt ccaatacaac
     7321 tacgcaggcc aatatttcaa cgactacgga ccaagctttg cctcttacgt ggtcggcatt
     7381 atcagtatca ccattgtcta tctcttcttc caacgccata tcatttcagg aatgagcaac
     7441 ggggcagtga agtaatactc aatgaaaatc aaagagcaaa ctaggaagct agccgcaggc
     7501 tgtacttgag tacggtaagg cgacgctgac gtggtttgaa gagattttcg aagagtataa
     7561 gaagagctca tggaatctat agtctttgac acagaggaag agttcagctt gttgataatt
     7621 ctatgtagaa aacaagaaag tagtactttg tttatcacag tctatattag tacgagtttg
     7681 atgataaata gagatttaaa atgaatttta tttatgaagc cagatcatat gagacttggc
     7741 ttcaattaga aaaaggagag taatgatgcc aattcaaaat aaaaccatgt tgattaccta
     7801 ttctgatagc cttggaaata atcttaaaga cttatatgat aatttggaag agcattttgg
     7861 agatgctatt ggaggagttc accttttacc atttttccca tcaacaggtg atcgtggatt
     7921 tgcgccagtt gactacgacg aagtggactc agcttttggt gattgggagg atgttaagcg
     7981 tttaggtgag agatattatc ttatgtttga ttttatgatt aatcatattt ctcgtcaatc
     8041 caagtattat aaggactatc aagaaaaaca tgaagccagt gaatttaaag atctcttttt
     8101 aaactgggat aagttttggc cagaaaaccg tccgacacag tctgatgtag atttaattta
     8161 caagcgtaag gatcgtgcac caaagcaaga gattgtgttt gaagatggtt cagtggaaca
     8221 tttgtggaat acctttggtg aggagcagat tgatcttgat gtgaccaaag aagtaactat
     8281 ggaatttatc cgtaagacca ttcagcactt ggcaagtaat gggtgtgatt tgattcgtct
     8341 agatgccttt gcttatgcag tgaagaaatt ggatactaat gatttctttg tggaaccaga
     8401 tatttgggat ttattggaca aagttcgaga tatcgctgct gagtatggga cagagctttt
     8461 acctgagatt catgaacact attcgattca gtttaaaata gcagaccatg attactatgt
     8521 ttatgatttt gctcttccaa tggtgacact ttatactctt tacagttcca gaacagagcg
     8581 tttggctaag tggttaaaga tgagcccgat gaagcaattt acgacgctag atacccatga
     8641 tgggattgga gtagtagatg tcaaggatat cctgaccgat gaggagattg actatgcttt
     8701 aaatgaattc tataaggttg gagccaatgt caaacgtaag tactctagtg ccgagtataa
     8761 caacttagat atctaccaaa tcaattcaac ctactattca gcgcttggag atgatgatgt
     8821 caagtatttt ctcgctcgtc taattcaagc ttttgcccca ggtattcctc aggtttacta
     8881 tgtgggtcta ttagcaggca agaatgactt gaaattatta gaagaaacta aagaaggtcg
     8941 aaatattaat cgtcattact atagcaacga ggaaatagca aaagaagtgc aacgacctgt
     9001 tgtgaaggcc cttctcaatc tattttcttt ccgtaaccgt tcagaagcct ttgatctaga
     9061 agggactact gagatagaga caccaacagc tcacagcatt gtaatcaaac gtcaaaataa
     9121 agataagtcc gtaacagcag tagcagaaat tgatttgcaa aatcagactt atcgggtaat
     9181 tgagaatgga gttgaagtaa cattttgaag ccttgatatg gtggataaaa tagggttttt
     9241 attttggaaa acgtttcctt tgttttcaaa ttgctaaaaa agtggtacaa tataggatag
     9301 cttactatta tctgaatcag ctgatttgga gagaaaggat tcattttgaa atcaataggc
     9361 tttattgaaa agctgaaggg gttgtctagt aaagagctga ttttattggg aattatcctg
     9421 agtatctttt taccctttta tctttttgta gttgtactct gtttatatat tatcagtttg
     9481 atttttacag gagacatgaa aagtattctt cagaaaatgg gggagcatcc gatgctgctt
     9541 ctttttctta gctatagtac tgttatatcc attcttgcac aaaattggat gggtcttgtg
     9601 gcttcagtag gaatgtttct atttactatt ttctttttgc actatcagtc gattttatcc
     9661 cataaattct ttcgattgat tttgcagttc gtcttgtttg gtagtgtctt gtcagctgct
     9721 tttgccagtt tagaacattt ccaaattgtg aagaaattta actatgcttt tctttcaccc
     9781 aatatgcagg tgtggcatca gaaccgggca gaagtgacct tctttaatcc taattattat
     9841 ggaattattt gttgtttctg tattatgatt gctttctatc tgtttacaac gaccaagttg
     9901 aattggttga aagtattctg tgtgattgca ggctttgtta atctctttgg tttgaacttt
     9961 actcaaaatc gaactgcctt tcctgctatt atcgctggag caattatcta tctctttacg
    10021 actattaaaa actggaaggc tttttggctt agtattgggg tcttcgcgat tggtttgagt
    10081 ttcctctttt ctagtgattt gggagttcga atgggtactt tagactcttc tatggaagaa
    10141 cgcatttcta tctgggatgc tgggatggcc ttgtttaagc aaaatccttt ttggggtgaa
    10201 gggccattga cctatatgca ctcttatcct cggatacatg ctccttatca tgaacatgcc
    10261 cacagtcttt atattgatac gattctgagt tacggaattg tgggtaccat tttattagtt
    10321 ctgtcttctg ttgctcctgt tcgtttgatg atggatatga gtcaggagtc ggggaaacgt
    10381 ccgattatcg gcctttatct atctttcctt acagtggttg ctgtgcacgg aatttttgac
    10441 ttggctctct tctggattca gtcaggcttt attttcttgc tagttatgtg cagtattcca
    10501 ttggagcatc gaatgttggt atcggacatg acggattaag ttttataagt ttgaaatctt
    10561 ctaccttggg taggagattt tttatacgat gggcatgtcg tgcatctgag gtgtaagtcc
    10621 tctgtaggga tccactaccg gtgaatacaa tagcgctccc caagcctgac tatcgtgagg
    10681 tagcggatta aaatggtctg gggatagatc gttttaagtc tgacgcagga aataagaatt
    10741 gtcagagaaa gcaatagtga aatcgtaggc tatgaataga aacgtgatag cgaggagcat
    10801 atagtggaca aggtagcgag aaattttaaa gtataaaaga tagtcttaat ctatatgtgt
    10861 aaatcacgaa agtaattgaa ttcggaataa ggttggtgtg aaaagacata gtaaactgaa
    10921 tcttgaatac tagaatgata cttctacaat attgttataa tttaatttga attccaaaat
    10981 cacattgttc cgtccgtatt tcattttact gtatgtcttc ctaagctcca aaatttctgt
    11041 gtcaaatttc ctattttcac tgtagtcttt gacacttcga acgacaattt gaatcatata
    11101 ttcaaaaaaa atgaaaaatt tgtgccaaca agcctaaata ttctgaaaat ttgtttgatt
    11161 tttaatggat aatgtgatat aatggttgtc aatctataat tcacataaac agaggagtag
    11221 aaaatgaaaa aaaatcgtgt atttgctaca gcaggtcttg ttttattagc agcaggtgta
    11281 cttgcagcat gcagttcttc aaaatcatct gattcatcag cccctaaagc ttatggctat
    11341 gtttatacag cagacccaga aaccttggac tacctgattt caagtaaaaa tagtacaaca
    11401 gtagtgactt caaatgggat tgatggttta ttcactaacg ataattacgg taatcttgct
    11461 cctgcagttg cagaggattg ggaagtctct aaggatggtt tgacctacac ttataagatt
    11521 cgtaaagggg ttaaatggtt tacctctgat ggagaagaat atgcagaggt gacggctaaa
    11581 gatttcgtga acggtttaaa acacgcagca gataaaaaat cagaagctat gtatttagct
    11641 gaaaattcgg ttaaaggctt ggcagattat ctatcaggaa cttcaacaga tttttcaaca
    11701 gttggtgtca aggcggttga tgattatacg ttacaataca ctttgaacca gcctgaaccg
    11761 ttctggaact ctaagttgac ctattctatt ttctggcctc tgaatgaaga attcgaaaca
    11821 tcaaaaggaa gcgattttgc taaaccaaca gatccgacat ccttgcttta taatggtcca
    11881 ttcttgttga aagggttgac tgcaaaatct tctgtagagt ttgtaaaaaa tgagcaatat
    11941 tgggataaag aaaatgtcca cctagatact atcaatctag cttactatga tggatcagat
    12001 caggagtcgc tagagcgtaa cttcactagt ggagcttata gttatgcccg tctttaccct
    12061 accagctcca actattctaa ggttgcagaa gaatacaagg acaatatcta ttacacacaa
    12121 tcaggctctg ggattgctgg tctgggtgtg aatattgatc gccaaagtta caactatact
    12181 tctaaaacta cagattcaga gaaagtagct actaagaagg cattgcttaa caaagatttc
    12241 cgtcaagcct tgaattttgc tcttgatcgc tcagcttact cagctcaaat caatggtaaa
    12301 gatggagcag ctttagcagt tcgtaattta tttgtaaaac cagactttgt ttcagctggt
    12361 gagaagacct ttggtgattt agtcgctgct caacttcctg cttatggtga tgagtggaaa
    12421 ggtgtgaatt tagctgatgg gcaggatggt ttattcaatg ctgacaaggc caaggcagag
    12481 tttgcgaaag ctaagaaagc tttagaagca gacggcgttc agtttcctat tcatctggac
    12541 gttccagtag accaagcatc aaaaaactat atatctcgta ttcagtcctt taaacaatct
    12601 gtagaaacag ttcttggtgt tgaaaatgtc gttgttgata ttcaacaaat gacaagtgat
    12661 gaattcctta atattactta ctatgctgcc aatgcttcat ctgaggattg ggatgtatcg
    12721 ggaggagttt catgggggcc agactatcaa gacccatcta cttacctgga tattttaaaa
    12781 acaactagca gtgaaactac aaaaacatat ttaggatttg ataatccaaa tagcccttca
    12841 gtagttcaag ttggtttgaa agaatacgat aaattagttg atgaagctgc caaagagaca
    12901 agcgacttga atgtccgtta tgaaaaatat gcagcggctc aagcatggtt gacagatagt
    12961 tcactcttta ttcctgctat ggcttcttct ggtgcagcac cagtgctttc acgaattgtt
    13021 ccatttactg gagcttctgc gcaaacaggc tctaaggggt cagatgttta cttcaaatat
    13081 ttgaaattac aagataaagc ggtgactaag gaagagtatg aaaaagctcg tgaaaaatgg
    13141 ttgaaagaaa aagctgaatc aaatgagaaa gctcaaaaag aattggcaag tcatgtgaag
    13201 taaataattt ctaatagaac tttctctcca tgaggagaag gttcttttgg gatttttaaa
    13261 ggaaataata tgaaaaaata tatttttatg cgtgttttgc ggtcattggt ttcgattttc
    13321 ttagtaacga ctttgaccta cacgattatc tataccttgg ttcctcgaaa attgattttc
    13381 aagcaggatc ctaactataa taaaattgcg acaacggctg ataaacgtga taactatgaa
    13441 aatactgtgt ttgagcgtat gggctacatt gagtattacg atactaaaga gttgcaagaa
    13501 aaggcaagta gcatggattc ttctgtaaca gtagaagcaa atgcgaccaa taaagctatt
    13561 tatgaaaagt acatcaatca attaggtcat ggttggactt tgggagaatt tactgaaagt
    13621 ggtcaattct atgctactcg tgaaattcca atttttgaac gtgtttttca cttctatgct
    13681 aacttgattg acattgacca tacaaataaa atccaagacc ctgaaaatcc agacttgaaa
    13741 cgctaccttc gttttgaaaa tgatccagct atcggatggt cattggtcgg ttcaggaact
    13801 aaacataaat atctcttgta ctttaacagt cagttcccat ttgttcatca aaactttgtg
    13861 aacttgaatt taggtgactc ttacccaacc tatgctaata caccagttct tcaggttatt
    13921 actcaaggtc aaggacaaac caaaactgcc caagttcagt tcccaacagg taagaaaacg
    13981 tcttctgtaa atatttactc aagaacctac aagtcaccta gtcaggctga ctctcgtgaa
    14041 gtagctagct atgggaaaga tgatccttat acagcgactg aaagtaatta ccaatatcca
    14101 tctatgattg tcagctctgc tattactggt ttgattggtt tggttcttgc ctatgctctt
    14161 gccgtgccac ttggttcagc catggctcgt ttcaagaaca cttggattga tagcctctca
    14221 acaggggctt tgaccttctt gcttgctctt ccaacgattg ccttggttta catcgttcga
    14281 ttgattggat catctattgc ccttccagat tcattcccta tcttgggagc tggagattgg
    14341 cgttcttacg ttttaccagc agtcatcctt ggtttgttgg gtgctcctgg tacagccatt
    14401 tggattcgtc gttacatgat tgacttgcaa tctcaagact ttgttcgttt cgctcgtgca
    14461 aaaggtttgt ctgaaaaaga aatttcaaac aaacacatct ttaaaaatgc catggttccg
    14521 ctggtttcag gaattcctgg tgccatcatt ggggttatcg gtggtgcaac ccttactgaa
    14581 acagtcttcg ccttcccagg tatgggtaaa atgttgattg actctgtaaa agcatctaat
    14641 aactctatgg tcgttggtct tgtcttcatc tttacatgta tttctatctt ctcacgtctt
    14701 ttgggagata tttggatgac tattattgac ccacgtatta aattgactga gaaaggaggc
    14761 aaataatgtc tacaatcgat aaagaaaaat ttcagtttgt aaaacgtgac gattttgcct
    14821 ctgaaactat tgatgcgcca gcatattctt actggaaatc agtgtttaaa caatttatga
    14881 agaaaaaatc aactgtagtc atgttgggaa tcttggtagc catcattttg ataagtttca
    14941 tctacccaat gttttctaag tttgatttca atgatgtcag caaggtaaac gactttagta
    15001 ttcgttatat caagccaaat gcggagcatt ggttcggtac tgacagtaac ggtaaatcgc
    15061 tctttgacgg tgtctggttc ggagctcgta actccatcct catttctgtg attgcgacag
    15121 tgattaactt ggttatcggt gtttttgtcg gtggtatttg gggtatttca aaatcagttg
    15181 accgtgtcat gatggaagtt tacaacgtca tctcaaacat cccacctctt ttgattgtta
    15241 ttgtcttgac ttactcaatc ggagctggat tctggaatct gatttttgcc atgagcgtaa
    15301 caacatggat tggtatcgcc ttcatgatcc gtgtgcaaat cttgcgctat cgtgacttgg
    15361 aatacaactt ggcgtcacgt actttgggaa caccaacctt gaagattgtt gccaaaaata
    15421 tcatgcctca attggtatct gttattgtga caaccatgac tcaaatgctt ccaagcttta
    15481 tctcatacga agccttcttg tctttcttcg gtcttggatt accgattaca gtgccaagtt
    15541 tgggtcgttt gatttcggat tattcacaaa acgtaacaac caatgcttac ttgttctgga
    15601 ttccattgac aacccttgtc ttggtatcct tgtccctttt cgtagttggt caaaacttag
    15661 cggatgctag tgatccacgt acacatagat aggagtagaa atgacaaaag aaaaaaatgt
    15721 aattttgact gctcgcgata ttgtcgtgga atttgacgtt cgtgacaaag tattgacagc
    15781 cattcgcggc gtttcccttg aactagttga aggagaagta ttagccttgg taggtgagtc
    15841 aggatcaggt aaatctgttt tgacaaagac cttcacaggt atgctcgaag aaaatggtcg
    15901 cattgtccaa ggtagtattg actaccgtgg tcaggatttg acagctttat cttctcacaa
    15961 ggattgggaa caaattcgtg gtgctaagat tgcgactatc ttccaggacc caatgactag
    16021 tttggacccc attaaaacaa ttggtagtca gattacagaa gttattgtaa aacaccaagg
    16081 aaaaacagct aaagaagcga aagaattggc cattgactac atgaataagg ttggcattcc
    16141 agacgcagat agacgtttta atgaataccc attccaatat tctggaggaa tgcgtcaacg
    16201 tatcgttatt gctattgccc ttgcctgccg acctgatgtc ttgatctgtg atgagccaac
    16261 aactgccttg gatgtaacta ttcaagctca gattattgat ttgctaaaat ctttacaaaa
    16321 cgagtatcat ttcacaacaa tctttattac ccacgacctt ggtgtggtgg caagtattgc
    16381 ggataaggta gcggttatgt atgcaggaga aatcgttgag tatggaacgg ttgaggaagt
    16441 cttctatgac cctcgccatc catatacatg gagtctcttg tctagcttgc ctcagcttgc
    16501 tgatgataaa ggggatcttt actcaatccc aggaacacct ccgtcacttt atactgacct
    16561 gaaaggggat gcttttgcct tgcgttctga ctacgcaatg cagattgact tcgaacaaaa
    16621 agctcctcaa ttctcagtat cagagacaca ttgggctaaa acttggcttc ttcatgagga
    16681 tgctccaaaa gtagaaaaac cagctgtgat tgcaaatctc catgataaga tccgtgaaaa
    16741 aatgggattt gcccatctgg ctgactagga ggaaggaaat gtctgaaaaa ttagtagaaa
    16801 tcaaagattt agaaatttcc ttcggtgaag gaagtaagaa gtttgtcgcg gttaaaaatg
    16861 ctaacttctt tatcaacaag ggagaaactt tctcgcttgt aggtgagtcc ggtagtggga
    16921 aaacaactat tggtcgtgct atcatcggtc taaatgatac aagtaatgga gatatcattt
    16981 ttgatggtca aaagattaat ggtaagaaat cgcgtgaaca agctgcggaa ttgattcgtc
    17041 gaatccagat gattttccaa gaccctgccg caagtttgaa tgaacgtgcg actgttgatt
    17101 atattatttc tgaaggtctt tacaatcacc gtttatttaa ggatgaagaa gaacgtaaag
    17161 agaaagttca aaatattatc cgtgaagtag gtcttcttgc tgagcacttg actcgttacc
    17221 ctcatgaatt ctcaggcggt caacgtcaac gtatcggtat tgcccgtgcc ttggtcatgc
    17281 aaccagactt tgttattgca gatgagccaa tttcagcctt ggacgtttct gtacgtgccc
    17341 aagtcttgaa cttgctcaaa aaattccaaa aagagctcgg cctgacctat ctcttcatcg
    17401 cccatgactt gtcggttgtt cgctttattt cagatcgtat cgcagttatt tacaagggtg
    17461 ttattgtaga ggttgcagaa acagaagaat tgtttaacaa tccaattcac ccatatactc
    17521 aagccttgct ttcagcggta ccaatcccag atccaatctt ggaacgtaag aaggtcttga
    17581 aggtttacga tccaagtcaa cacgactatg agactgataa gccatctatg gtagaaatcc
    17641 gtccaggtca ctatgtttgg gcgaaccaag ccgaattggc acgttatcaa aaaggactaa
    17701 actagtaatg gttttataat ttccatgtca atttttatgg aaattataaa actttttttg
    17761 ttggactgat ttgaaaaact ttgaaagctt cctgaaagaa tttttgaaaa attttaaaaa
    17821 ttatgaaata ttattgacag gtgacgaaaa aggtggtagg ataggaagca tcaaaaaaga
    17881 aagcagaaaa agaaatgaat aagagacaag ccgtaactat gaatcaaaac ttttacttta
    17941 gctactttag atagtgtttg tagacatgtc accatggatg caaacagttc aagcaatttg
    18001 tttgtatcta tgtggctaag atttttgatt gtggtccata tagatgaata tctaaatgga
    18061 ctagctaagt tgtaacttgt taggttattt caagtatccg tttagatagt atctaggcgg
    18121 ttttttgttt tatcttttct gagaaggagt ttcattatga aagttgttcg aaaatcacta
    18181 gccccctctt ggtagtgggg atcctcttga cttctctgat cagtttgcat cagttgaagg
    18241 cagataagaa aagagatgtc tttcgtatcg gtatttcgca gtttattacc caccagtcct
    18301 tagacgctac tagagaagga tttgtggatg agctggccaa acaaggctat gttgaggggg
    18361 aaaatattga gattgatttg caaaatgcac agggagaaca aagaaatcta aaaacgattt
    18421 cccagcaact agcagaatcc agcgatgtcg ttctagccat cgcaaggcct tctgctcaga
    18481 gcttggccaa tacgacgcaa acgacaccag ttatcttttc agctgtaaca gacccagtca
    18541 gtgccaagtt ggttgagtca agagaacatc ctgggggcaa tgtaactggg acaagcgatc
    18601 agtcatcaga tgccatttca acccaaatca atttgataaa gaaagtgttg ctaaaggcta
    18661 aaacgattgg aatcctctat actcagagcg agcccaattc ggttgtttaa aaggatgaag
    18721 ctaagcgact tctgaaagaa aaaggcttta ccgttgttga aaaaacaatc ttggacagta
    18781 ataacatcaa ggcggcagca gagagtttga tggcagaggt ggatatggtt tttgtcccaa
    18841 ctgataatat catttcgtca accatggaaa cagttaagca ggtttctatt aaacacaagg
    18901 ttccagtatt cggtggttca acagaaatga ttgctgttgg tgacttgtat aactacggaa
    18961 ccaattatga agaattggga agacagacag ctcgcatgct gattcgtgtt ttaaaaggtg
    19021 agaaaccaga aaatatagca gttgagttgc ctgaaaaact ggaattgcat actaatcaag
    19081 aaatggcaga tgcattggga attgatatta gtaagttaga aggcaaggaa taaggaggat
    19141 taagatgaat tttgtgttat ctagtttatc agaaggtttg ttgtggtcga ttgtggcgat
    19201 tggggtctac ttgactttcc gtattttgga tattgcggat atgactgctg agggagcctt
    19261 tcctctgggg gcagcagtcg ttgtatcaca gatacaggca ggggcaaatc cttggcttgc
    19321 gattttactt gctttgctgg caggtatggt agcaggtctt gtatcaggaa tgctccacac
    19381 caagatgaaa atcccagctc tcttgacagg gattgtgacc ttgacagggc tctattcaat
    19441 caatattaaa atcatgggca gtgtactcaa tctttccttg ggagattctg caaccgtctt
    19501 taaacagttg gcgagcttgg gtctgacaaa tgaaggagct gttttctcac ttagtttagt
    19561 ctgtctctta cttgtttggt cttgaccctt ttgatgaaaa cagagattgg tttggtcttg
    19621 cgttcgactg gggataatat cccgatgagt gaggccaatg gagtcaatgt agacaccatg
    19681 aagattgttg gttacatgat ttcaaacggt ttgattgccc tatgtggttc cttgtttgcc
    19741 caaaatgatg gattttcaga tgtgacttct gggacaggaa ccattgttgt tggtttgagt
    19801 tcggtgatta ttgcggaagt cttgatacac gacttgacca ttggaggccg cttattatcc
    19861 atcggaatcg gtgctattgt ttaccgtttg attattttaa atatctatga aattccaaat
    19921 ctagatcaaa acttggtacg tctctttaat gcaatcttgc tcgctttggt tttatttgca
    19981 ccagaattgc aaaagagatt aaagattcgt ggtctgaaat tgagaaatga ataggaggag
    20041 aaagttatgg caagtttgtt aacacttgaa aatattcaca agacatttga agcagggacg
    20101 gtcaatgaga accatgtcct caaaggatta gatttagagg ttgaagaggg agattttatc
    20161 tctgtgattg gtggaaatgg agcaggaaaa tccactttga tgaatatctt ggctggcaat
    20221 ctatcgatgg acgaagggga ccttctactg gcagggaaat ccattaaaaa tctgagtgtc
    20281 agaaagagag ccaaggatat tgcccgtgtt tttcaagctc ctaagatggg aacagcttct
    20341 cgtttgacga ttgaggagaa tatggctatt gccttgagac gtgggcaaaa gagagggctt
    20401 ggttggggcg tgaaggagaa ggatagaatc cagttccaag aggccttgaa ggagttgaat
    20461 attggtcttg aaaaccgctt aaaagtagat actcaatatc tctcaggagg acaaagacag
    20521 gctttgaccc tagtcatggc agctctggtg aaacccaaac ttttgctttt ggatgaacat
    20581 actgcggcac ttgacccgaa aaccagtcaa atggtgatgg acttgacgca aaagattgtg
    20641 gagcaccatc agttgacgac tttgatgatt acccatgata tgaatcatgc gattgagtac
    20701 ggcaatcgtc tcattatgct ctatcaaggc aagatagtgg tggatgtcaa gggagaagag
    20761 aaaaagcatc tgacagttga agaccttatg catctcttcc agaaaaatag tggccaaagt
    20821 ctggtcagtg atgaattagt tttgagataa agaaaaaggc tgagattggt gtctcagtct
    20881 tttatttctt gcgttttgca aaatctacaa atctaaattt ttctaactta tggcgacttt
    20941 cggtaaactg aaattggcgt ccgtcttgga gatagacttt tgatttaatc gaaacgacat
    21001 aagggtcttt gccaatgtcc atgagaatct tgtctcggtc gcttgagtgg tcaatggtga
    21061 tttccttctg agcataatca ataaggagtt tgaggccatt ttctatataa gaatagatag
    21121 actgctcagc aatttggcga gtgagatttg ggatgagttc catatccaga tagtctgtat
    21181 ccagaacgga taccagatca tccaccacac gctggcggac caccttccaa accatccgaa
    21241 actctgggaa accggttatc agtgaggatt ttttatcaat aataatcttg tccagactga
    21301 ccacgttggt tttagagcgc agtccaagtt ctttaactag ttcttggtag ctggttaggt
    21361 tggatacagg gaaattgacg gtttcttctt tgacgacttg agaaccttgc cctcttatct
    21421 ttttgatcaa tccttcctct tggaggagag acagggcctt tcggacggta tcacgactca
    21481 cttgatattg ctccataagg tcgtgctcgc taggaaggaa atctccgaca gcgtaagtct
    21541 cgttttgaat ggtttcttgg atttgcttaa ataattgttg gtatttcttc attttgcctg
    21601 cttaaatagg tttttagtct agtttaatga acacctgtat ctattttacc atacaagtgc
    21661 tagaaaattt acaaaaaaac acccttttta taccaaacaa gttgcagaca agtttggtat
    21721 cgtttacaat atacttatca aatcaaactt gctttgacaa gtataaggag aatcaaatgg
    21781 gaaaatttga acaagaagcc aaagatctgc ttcaggcaat tggaggcaaa gaaaatgtga
    21841 ctgccgtcac ccactgtgcg acacggatgc gctttgtttt aggagatgat aagaaggcta
    21901 atgttaaagc tatcgagtca attccagctg ttaaaggaac ttttacaaat gcaggtcaat
    21961 ttcaggtaat cattggaaat gacgtgccca tcttttataa tgattttaca gccgtttcag
    22021 gtattgaggg tgtttccaaa gaagcagcca agtctgcagc taagagtaat caaaacgtgg
    22081 tccaacgtgt tatgaccact ctggcggaga tttttactcc gattattcca gccttgatag
    22141 tcggaggatt gatcctcggt ttccgtaatg tcttggaagg tgtccattgg tcgatgttgg
    22201 atggcaagac catcacagaa tcctctcagt tttgggcagg tgtcaatcac ttcctctggt
    22261 tgcctggtga agctatcttc cagttcttac cagtagggat tacttggtct gtttctcgta
    22321 agatgggaac cagccaaatt ttgggaattg ttctcggaat ctgtttggta tcgcctcagt
    22381 tgctcaatgc ctatgcggtt gcttcaacgc cagcagctga tatcgcggca aactgggttt
    22441 ggaattttgg ctattttact gttaatcgta tcggttacca agcccaagtt atcccagcct
    22501 tgcttgcagg tttgagtctg tcttatcttg aaatcttctg gcgcaagcat atcccagaag
    22561 tcatttctat gatttttgta cctttcttgt cattgattcc agccttgatt ttggctcata
    22621 ctgttttggg accaatcggt tggacaattg gacaaggact ttcatcagtt gtcttggcag
    22681 gtttaactgg tccagttaaa tggctcttcg gtgcaatttt tggtgccctc tacgctccat
    22741 ttgtcatcac aggtctgcac catatgacca atgccattga tacacaattg attgcggatg
    22801 ctggtggcac tgccctatgg ccaatgattg ctctttctaa tattgctcaa ggctcagccg
    22861 tgtttgccta ttatttcatg catcgccatg atgagcgtga ggctcaggtt tcacttcctg
    22921 caaccatttc agcctatctc ggtgttacag aaccagctct ttttggggtt aacgtaaaat
    22981 atatttatcc atttgttgct gggatgactg gttcagccct tgcaggcatg ttatccgtta
    23041 cttttaatgt aactgcggct tctattggta tcggtggttt gccaggtatt ctctctattc
    23101 aacctcaata catgctgcca tttgcaggaa ctatgctagt tgcgattgtt gttccaatgc
    23161 tcttgacttt cttcttccgc aaggctggtc tctttacaaa aacagagggc gatacgaact
    23221 tgcaggcaga attcgttgct caagaagaag cagaatttgt gaaccatgaa ccagtagaac
    23281 ttacttcggt agaaattatc agcccactaa ctggccaagt gaaagaattg agtcaagcga
    23341 cggatcctat ttttgcatca ggtgtcatgg ggcaaggtct agtcattgaa ccaagccaag
    23401 gtgagttgac ctctccagtt aatgggacag tgacggttct tttccctacc aagcatgcca
    23461 tcggcattgt ctctgacgag ggagttgaat tgctcatcca catcggtatg gatacagtag
    23521 gtcttgatgg caaaggtttt gaaagtcttg tagtccaagg agatcacgtt acagttggtc
    23581 agcaactgat tcgttttgat atggatgtca ttaaggctgc aggtctggtg acagaaactc
    23641 ctgttatcat caccaaccaa gatgcttata cagcgactat tcccggaact tatccgacaa
    23701 cgatccaagc tggagcatct ctcatggtcg ctacacgaat ctaatactct tcgaaaatct
    23761 cttcaaacca cgtcagctct atctgcaacc tcaaaatagt gttttgagca acctgtggct
    23821 agcttcctag tttgttcttt gattttcatt gagtataatt gaaatagcac aaatagggac
    23881 ggaagactgc ttcctccctt ttatcagaaa aggagaaaca gatgacactt gataaaggaa
    23941 aagtagtcta tcaaatctat ccaaaatctt acaaagacac cactgaaaac ggtattgggg
    24001 atttccgtgg gattatcgaa aaaattccct atttagccaa actaggtgtg gatatggtct
    24061 ggctcaatcc attctatcca agccctcaac gggataatgg ttacgatatt tcagattata
    24121 tggcagtgga tcctcttttt ggtgatatgg ctgattttga ggaaatggtg tgtgtcggta
    24181 aagagcacaa gattgacttt atgctggaca tggtacttaa tcattgttcg acagagcatg
    24241 aatggtttca gaaagcccta gctggtgata agtattatca agactttttc ttcatccaag
    24301 accaaccaac agattggcag tctaagtttg gtggttctgc atgggcgcct ttcggggata
    24361 ctgggaaata ctatcttcac ctatttgatg agacccaggc tgaccttaac tggcgcaatt
    24421 ccaatgtccg taaggagctt ttcaaggttg ttaatttctg gcgcgacaag ggtgtcaaag
    24481 gtttccgatt tgatgtgatc aatttgatcg gtaaggacga ggtttcagtg gactgtcctg
    24541 aaaatgaagg gaagccagct tatacagaca aacccatcgt tcataactat ttgcgtatga
    24601 tgaaccaagc cacttttgga tctgacgata gctttatgac agttggggaa atgtcttcta
    24661 ccaccatgga aaactgtgtc ctctattcgt cgcctgatcg tcaggaatta tccatgacct
    24721 ttaattttca tcacctcaag gtggactata aggatggaca aaagtggacc ttggctccct
    24781 ttgattttga agagttgaag agtctttacc atagttgggg taaggaaatg agtgataaag
    24841 acggttggag tgccctcttt tggaacaatc acgatcaacc acgtgccttg aaccgttttg
    24901 tcgatattca aaacttccgc aaggaaggag ctaccatgct agcagccagc attcacctgt
    24961 cacgtgggac accttatatc tacatgggtg aggaaatcgg gatgattgac ccagactatg
    25021 attctatggc tgattatgtg gatgtcgaat cgcttaatgc ctatcagatg ctcttagaag
    25081 aaggaaagag tcagcaagaa gccttccaga ttattcaagc taagtcgcgt gataattcac
    25141 gtattcccat gcagtgggat acttctgaaa atgcaggatt ttcaacaggc actccatggt
    25201 tgaaagttgg taaatcctat aaagatatca atgtagaaaa tgaaatcaaa ggtctgattt
    25261 tcaccttcta tcaagacttg attcgactgc gtaaggaaat gccaatcatt tcagaaggaa
    25321 attacaaacc agcctttgaa gatagcaaac aagtctacgc ttttgaacgc cagtttgagg
    25381 atcaaaagtt actagtgctc aataactttt atgccaaaga agtggaaatc gacttgccag
    25441 cagtctacca aaatggacag attttgattt caaactatga agatgcagaa gtgtctgaga
    25501 aaattctact caaaccctat caaacactgg ctatttatgt aaattaatac tcttcgaaaa
    25561 tctcttcaaa ccgtgtcagt tttatctgta acctcaaagc ggtgctttga gcaacctgtg
    25621 gctagcttcc tagtttgctc tttgattttc attgagtata aacttagcaa ggagacctct
    25681 ttttcagaaa agcagggttc tttttgctgt ctattccatc cttgaaagtg tagaacaaac
    25741 tggtcagggt ggctagtttg tggtataatg aaagggactc aaaaagaaac aggagaaaaa
    25801 tgatggattt acttttagca attgtattga ttgtgctagc ttttctagga ggagctcttg
    25861 gaggaatgta cttggttcgt aagcaaatcg aaaaagaatt tgctgacaac ccacgtttga
    25921 atgctgaagc agttcgtact cttttgagtg caaatggtca aaaaccaagc gaagctaagg
    25981 tacaacaagt ttaccaccaa atcatccgcc aacaaaaggc agcccttgct aacaataaaa
    26041 agaaaaaata aataaggaaa agtctgggat gaaagttcca gacttctcac tatgttggtt
    26101 atggtttagg gatgagactt tttccttgca ttctattgat atttgattat gattaagaga
    26161 gaggtagttg acattctatc aagctgtcgg tcagttcgtt cagtacaagg aatcataatg
    26221 aacgatcaat cagaaaaaag actagaaaga agactgtatg gataatcgac caattggttt
    26281 tttggattcg ggtgtcgggg gcttgaccgt tgtgcgcgag ctcatgcgcc agcttcccca
    26341 tgaagaaatc gtctatattg gagattcggc gcgggcgccc tatggccccc gtcctgctga
    26401 gcaaattcgt gaatatactt ggcagctggt caactttctc ttgaccaagg atgtcaaaat
    26461 gattgtcatt gcttgtaaca ctgcgactgc ggtcgtctgg gaagaaatca aggctcaact
    26521 agatattcct gtcttgggtg taattttgcc aggagcttcg gcagccatca agtccagtca
    26581 aggtgggaaa atcggagtga ttggaacgcc catgacggta caatcagaca tataccgtca
    26641 gaaaatccat gatctggatc ccgacttaca ggtggagagc ttggcctgtc ccaagtttgc
    26701 tcccttggtt gagtcaggtg ccctgtcaac cagtgttacc aagaaggtgg tctatgaaac
    26761 cctgcgtccc ttggttggaa aggtggatag cctgattttg ggctgtactc attatccact
    26821 ccttcgccct attatccaaa atgtgatggg gccaaaggtt cagctcatcg atagtggggc
    26881 agagtgcgta cgggatatct cagtcttact caattatttt gaaatcaatc gtggtcgcga
    26941 tgctggacca ctccatcacc gtttttacac aacagccagt agccaaagtt ttgcacaaat
    27001 tggtgaagaa tggctggaaa aagagattca tgtggagcat gtagaattat gacaaataaa
    27061 atttatgaat ataaggatga ccaggactgg tatgttgggt cttatagtat ttttggtggc
    27121 gttaacagtt tgagcgacta taagacagat tttcctctgt ttgaattctc caaaatattt
    27181 ggagatgaag agtatggttt cccgctttca gttactgttt tacgctatgg ttctacctac
    27241 cgtttgttct cctttgtggt agacatgctt aatcaagaaa tgggacgaaa cttggaagtt
    27301 attcaacgtc atggggccct gctcttggtt gaaaatgggc aactcttgta tgtagaattg
    27361 cctaaagaag gggtcaatgt tcatgatttc tttgagacaa gcaaggtcag agaaaccttg
    27421 ttgattgcga ctcgtaacga aggtaaaacc aaggaattcc gagctatctt tgataagtta
    27481 ggctacgatg tggaaaatct taatgactac cctgacctgc ctgaagtagc agaaacaggt
    27541 atgacctttg aagaaaatgc ccgccttaag gcagaaacca tttctcaatt aacgggcaag
    27601 atggttttgg cagatgattc tggtctcaaa gtcgatgtcc ttggtggctt accaggcgtc
    27661 tggtcagctc gtttcgcagg tgtgggagca actgaccgtg aaaataatgc caaactcttg
    27721 cacgaattgg ccatggtctt tgaactcaag gaccgctcgg ctcagttcca cacaacccta
    27781 gtcgtagcca gcccaaataa ggaaagttta gttgttgaag cagactggtc aggttatatt
    27841 aactttgaac ctaagggtga aaatggcttt ggctatgatc ccctcttcct tgtaggagag
    27901 acaggtgagt catcagctga attaaccctg gaagaaaaaa atagtcaatc tcaccgtgcc
    27961 ttagccgtta agaaactttt ggaggtattt ccatcatggc aaagcaaacc atcattgtaa
    28021 tgagcgattc ccatggcgat agcttgattg tggaagaagt ccgtgatcgc tatgtgggca
    28081 aagtcgatgc cgtttttcat aacggcgatt ctgaactacg tccggattct ccactttggg
    28141 agggcatccg cgttgttaaa gggaacatgg acttctacgc cggctaccca gaacgtctgg
    28201 tgactgagct tggttcgacc aagattatcc aaactcatgg tcacttgttt gacatcaatt
    28261 tcaactttca aaagttggac tactgggctc aggaggaaga ggccgctatc tgcctctatg
    28321 gtcacttgca tgtgccaagt gcttggatgg aaggcaagat cctctttcta aatccaggtt
    28381 ctatcagcca accacgaggt accatcagag aatgtctcta tgctcgtgtg gagattgatg
    28441 atagttactt caaagtggac tttttgacac gagatcacga ggtgtatcca ggtttgtcca
    28501 aggagtttag ccgatgattg ccaaggagtt tgagactttc ttgttggggc aggaggaaac
    28561 ttttttgacc cctgctaaaa atctagctgt gttgattgat acccacaatg cggatcatgc
    28621 gaccctcttg ctcagtcaga tgacctatac ccgtgttccc gttgtgacag atgaaaaaca
    28681 gtttgttggg acgattggac tcagagatat tatggcttat cagatggagc atgacttgag
    28741 ccaagaaatc atggcggata cggatatcgt tcatatgaca aaaacggacg tagcggttgt
    28801 ttcgcctgat ttcaccatta cggaggtctt gcacaagcta gtagatgagt ccttcttacc
    28861 ggttgtggat gcagagggta ttttccaagg gattattacg cgcaagtcca tcctcaaggc
    28921 cgttaatgcc ctcttgcatg actttagtaa ggaatatgag attcgatgcc aatgagagac
    28981 aggatttcag cctttttaga ggaaaagcag ggcttgtctg tcaattccaa gcagtcctat
    29041 aagtatgatt tggagcaatt tttagacatg gtaggtgagc ggatttctga gaccagtctc
    29101 aagatttacc aagcccagct agccaatcta aaaatcagcg cccagaagcg aaagatttcg
    29161 gcctgtaacc aatttctata ctttctctat caaaaaggag aggtggacag cttttaccgc
    29221 ttggaattag ccaaacaagc tgaaaagaag acggaaaagc cagagattct atacctagac
    29281 tctttttggc aggaaagcga ccatccagag ggccgcttgc tagcgctctt aatcctagaa
    29341 atggggctct tgcccagtga gattttagcc atcaaggttg cggacatcaa tctggatttt
    29401 caggtgttgc gaatcagcaa ggcttcccaa cagaggattg tcaccattcc cacggccttg
    29461 ctttcagaat tggaaccctt gatggggcag acctatcttt ttgaaagagg agagaaaccc
    29521 tattctcgtc agtgggcctt tcgtcagtta gaatcttttg tcaaggagaa aggttttcca
    29581 tccttatcag ctcaagtctt acgtgaacag tttattctaa gacaaataga aaacaaggtc
    29641 gatttgtacg aaattgcaaa aaaattagga ttaaaaacag tcctgacctt agaaaaatat
    29701 agataatgga tattaaatta aaagattttg aaggacccct ggacttgctc ttgcatctgg
    29761 tttctaagta ccagatggat atctacgatg tgcccattac ggaagtcatc gaacagtatc
    29821 tagcctatgt ctcaaccctg caggccatgc gtctggaagt gacgggtgag tacatggtca
    29881 tggctagtca gctcatgctg attaagagtc gtaaactcct tccgaaggta gcagaagtga
    29941 cagacttggg ggatgacctg gagcaggacc tcctctctca aatcgaagaa tatcgcaagt
    30001 tcaagctctt gggtgagcac ttggaagcca agcaccaaga acgggcccag tattattcca
    30061 aagcgccgac agagttgatt tacgaagatg cggagcttgt gcatgacaag acgaccattg
    30121 acctcttttt ggctttttca aatatcctag ccaagaaaaa agaggagttt gcacaaaatc
    30181 acacgacgat cttgcgggat gagtataaga ttgaggacat gatgattatc gtgaaagagt
    30241 ccttgattgg acgagatcaa ttgcgcttgc aggatttgtt caaggaagcc cagaatgtcc
    30301 aagaggtcat caccctcttt ttggcaaccc tagagttaat caaaacccag gagctgatcc
    30361 tcgtgcaaga ggagagtttt ggagatatct atctcatgga aaagaaggaa gaaagtcaag
    30421 tgcctcaaag ctagacttga tagagaggaa agatgagtac tttagcaaaa atagaagcgc
    30481 tcttgtttgt agcgggtgaa gatgggattc gggtccgcca gttagctgaa ctcctctctc
    30541 tgccaccgac aggcatccag caaagtttag gaaaattagc ccagaagtat gaaaaggacc
    30601 cagattccag tttggctttg attgagacaa gtggtgctta tagattggtg accaagcctc
    30661 aatttgcaga gattttgaag gaatactcta aggcgcctat caaccagagc ttgtctcggg
    30721 ctgcccttga gaccttgtcc attattgcct acaaacagcc gattacgcgg atagaaattg
    30781 atgccatccg tggagttaac tcgagtggag ccttggcaaa gttgcaggct tttgacctga
    30841 taaaggaaga cgggaaaaag gaagtattgg ggcgccccaa cctctatgtg actacagatt
    30901 atttcctaga ttacatgggg ataaaccatt tagaagaatt accagtgatt gatgagcttg
    30961 agattcaagc ccaagaaagc caattatttg gtgaaaggat agaagaagat gagaatcaat
    31021 aagtatattg cccacgcagg tgtggccagt aggagaaaag cagaagagct gattaagcaa
    31081 ggcttggtga cggttaacgg tcaagtggtg cgtgaactag caaccactat caagtcaggc
    31141 gacaaggtcg aagttgaagg tcaacctatc tacaacgaag aaaaggtcta ctatctgctt
    31201 aacaaaccac gcggtgtcat ttccagtgtg acagatgata agggtcgcaa gacggttgtc
    31261 gacctcttgc ccaatgtcaa agagcgtatt taccctgtgg gtcgtttgga ctgggataca
    31321 tcaggagtct tgattttgac caatgatggg gactttacag acgagatgat tcaccctcgt
    31381 aatgagattg acaaggttta tgtcgcgcgt gttaaaggtg tggccaataa ggacaatctt
    31441 cgccccttga cccgtggtct tgagattgat ggtaagaaaa ccaagccagc tgtttatgaa
    31501 attctcaaag tggacccagt caaaaatcgc tctgtggtgc agttgaccat ccatgaaggg
    31561 cgtaaccatc aggttaaaaa gatgtttgaa gctgttggtc tccaagtaga taagttgtct
    31621 cggactcgtt tcggacacct agacttgaca ggactccgtc caggagaatc ccgtcgtctt
    31681 aataaaaaag aaatcagcca actacacacc atggctgtaa ctaagaaata atgaaacgaa
    31741 ttttaatagc gcttgtgcgc ttttaccaac gttttatctc accagtcttt ccaccctctt
    31801 gtcgctttga gctgacttgt tccaactaca tgattcaggc tattgaaaaa catggtttta
    31861 agggggtatt gatgggcttg gctcggattt tacgttgtca tccctggtcg aaaacaggta
    31921 aggaccccat tccagaccac ttttccctta aacgaaatca agaaggggaa tgaggtgggg
    31981 taaatagatt tcaaaatgat aaaaacgcat cctatcaggt ttgagtaaac ttgataggat
    32041 gcgttttaga atgtcaaaat tttatactct tcgaaaatct cttcaaaccg cgtcagcttt
    32101 catctgcaac ctcaaaacag tgttttgagc aacctgcggc tagtttccta gtttgctctt
    32161 tgattttcat tgagtattaa attgagtttg aagtggctta tttcaaagct ttttgtatgt
    32221 cttcaatcat gagttttgtt gattcaagtc cgcctccgct tagataccag aggtctggtg
    32281 ttagttggat aatcttacca tttttagcag caggtgtttc agcgataagg gcattttcta
    32341 ggacaccgtc gttgctagag ttgtccccac cgatggcaag ggtacggttg atgacaaaga
    32401 ggatgtcagg gttgatttct ttgacacttt caaagctgac ttcttgtccg tggcgtgagt
    32461 cttcaaattt tgtatcagtt ggtttgaatt tcaaggtttg gtacaagaaa gagaaacgag
    32521 atttggcacc aaaggctgcc atttttcctt cattaaggag gatcgcaagg gcttttttgt
    32581 cagagctttc atttttagta gcgacttctt ggatgctctt gtctagcttg gccaattctt
    32641 ccttggcttt ttgtgtacta gtttcgccga aggcacttgc taaggattcg atattagcct
    32701 tggtagaagt ccagtagtca tccttgcttg cttggaagag aacggttggg gcgatttctt
    32761 tgaatttgtc tacgaatttt tgtgtacgtg gtgaagcgat aatcaaatca ggctcaaggg
    32821 cggcgatagc ttctaaatca ggttctttca tagaaccaac atttttgaca gttcccacta
    32881 ggtcttttag ataagtcgga acagtttttg taggcattcc gacgatattt ttttcaaatc
    32941 ctaaagcgcg aatagtatcc gcagcgccga ggtcaaaggt cacaatcttt tcaggaactt
    33001 tggaaagttt gacctcgtcc agtgaacttt taatggttat ctctgttgga gcagagctac
    33061 tggtctctgt ctgactagtg cttgagtttg tactacatgc accaagtagg agcaagaagc
    33121 tggccactag ggcagtgaaa taaagtttaa gggatgtttt cataatttct cctttttaaa
    33181 atgtgataac gatttaggga gtctcttaat cttattgact aagagactga aggttctcta
    33241 acttgagctt ttatgttact agctatagat acagatcttt ttgttattga tatcagctag
    33301 cgtgatggga atctcataaa gttgactgag caggtcagcc tgcatgattt gatcggttct
    33361 tcccttgcta aagacctggc cgtccttgaa ggcgacaatt tcatctgcat actgactggc
    33421 catgttgata tcgtggagga cgatgataat ggtcttgccg agttcctcca ccagtcgtcg
    33481 aagaatctgc atcatgctga cgctttgctt gatatcgaga ttgttgagtg gttcgtccag
    33541 caagataaag tccgtatcct gggccagtac catagcgata aagacgcgct ggagttgccc
    33601 ccctgacagg ctattgatgt agcggtcttt taagttggtc agttccaaat agttcagagt
    33661 ttctcggatt ttttcccagt cttctgatct aagtcgacct cggctgtagg gaaaacgtcc
    33721 aaaactgacc agttcttcaa cagtcaattt ggcttggtaa ttgattttct gttttaggat
    33781 ggttagttct tgagccagtt cttgcgaatt ccagctctcg atttcacgtc ctttgatact
    33841 gagaactccc tgatctttct tggttagtct gctcatgatg gagaggagag tcgattttcc
    33901 agcaccattt ggaccaataa aggctgtcag tttttgagga ctgacttcaa gcgaaatgcc
    33961 ttgcaaaata tcctgttttt gaatggattt gtcaatgttt tccagtttca ctgacgagcc
    34021 ctcctatata gtaagataaa gaataagaag ccacccacac tctcaatgat catactgata
    34081 cgaatttcca gtgcaaagac tcgttcaatt aaggcttgcc ccaaggttaa gctaataaat
    34141 ccaaccagaa tggccactat aaagagtaac ttgtgctgat agtctttgac aatcaggtag
    34201 gtgaggttgg ccagcataaa gctgaagaag gccataggtc ctaccaaggc agtggccgtt
    34261 gaggtcaaaa gcacgattcc ccagagtaac tctttctgtt ctttttcaac atcgagtccc
    34321 aatatctgag ccgtttctct ttgcaggtgc aagacatcta gaacgactgc ttttcgaaag
    34381 aaaaagattg tcaaagcgag gatgatcaga gaaccgatgg ctaggatgga agtgttgaga
    34441 tgttgaaagg aggcaaaaag actattttgc agtttatcgt attcgtttgg atccattagg
    34501 acttgaagga aggtgctgat atttcgaaag agacttctga gcgctagaca gatcagcagg
    34561 acgaagacca ggtcttgctt catcagtgtc ttcaagtaac cttgtaaggc gagaaagaag
    34621 agggactgga caagaagtaa gactaggaat tctaagatag gggatttgcc aagttgaaga
    34681 aacttgcttt caaaaaccag tagtagggtt tgtagtagga cgtagaagga ttcaattccc
    34741 aaaatactag gcgtcaggaa gcgattttcc gtcagggttt gaaaactaat ggtcgaaatc
    34801 ccagtcgcga tggctaccaa gagataaacg atgatctttt gggaacgcaa cttccaagca
    34861 aaggctgaca agtgagtgat gggccaaaag tagagaagac aagctccgat ggcaagaata
    34921 atgagaatcc agaagagctt ggtatgtttg cttttagtct gcatcttttc gtccccctct
    34981 ccagagaagt aggataaaga cgagactacc gatgattcct agcaagagac tgacagacaa
    35041 ctcatagggc ctaatcagaa ctcgggatag gatatcgcaa gccagaacta gattggcacc
    35101 aaccagtgcg accatgagtt tggtttgact tagattatct ccatagcgct tgcgaacaag
    35161 attgggaacg ataactccga gaaatggtag gtcacccacg gtaatcatgg tgacgcttgt
    35221 cgttagcgcc accagaaaga gggccagttt ttcaagtagg gagtaggaaa tccccaaact
    35281 ctcgctggtt tctttcccta gattcatgat ggtgaaggtt tgggataatt tccaaacggt
    35341 tatcaggatg atgaggccta agaagagcca ctcatactga tgggtctgaa tcatggagaa
    35401 ggagccctgg gtccaggcag tcatactctg aaccagattg aaacgatagg cgataacttc
    35461 tgtgactgag ccgataatcc cgctatagat gatcccaatc agaggcaaca tccacctttc
    35521 ctttacagta aaaatggtca taaaggctag gaagaagagg gtgaatacga tggatgaaac
    35581 aaaagcgaag agcatctttt gggtcagact agccgatgga aagacaaaaa ggctcagcac
    35641 cattcccagt ttggcggctt cagtcgttcc aactgtactc ggtgcagcaa actgattttg
    35701 ggtaatagtc tgcatgagaa ggcctgccat actcatacta gaggcagtca ggagaatact
    35761 gatagttctt gggagacggg actcttgaaa gaggagccag gtctgctggt cgaaatcaaa
    35821 tagctttccc catgaaaaat cactggtccc aatgctaata gagagaaaga ctaggagtag
    35881 aagtaagcca attaaataat gagaaagttt cataccccgt cctttcatgt agatttggta
    35941 tcgaaagata tctgcggata taaatgtaac attatttttc taatctgtca ataaaatttc
    36001 tgacaattta ataaatacaa caaggagaga gcaacaagac tttctccttt gttatcctat
    36061 tctaaaatgt ttttacctta atctgataaa ataatatctt cgagggagta gctagccgtc
    36121 caatcaagat attgtttagc ttttgaagca tctgctagga cactggctgg gtcactagca
    36181 cgtcgagcaa caatctcgtg tgggattttt taatttagta attcttcagc agttttaaag
    36241 atttctttga tagtatagcc ttttttagtt cctaagttaa agatttgaga agaactgtct
    36301 tcttgaaata ggtagttcat tcctttaaca tgagcctatg caaggtccaa gacataaatg
    36361 taatctcgaa tacatgaacc gtcacgtgta tcgtagtcat ctccaaatat ttttaagcta
    36421 tcatgttgtc ccaatgcggt cttgttgata tttggaatga tgtgagttgg atttttcaca
    36481 cgcagaccgt ttgaagcatc catttcagcc ccagcaacat taaagtaacg gaaaataaca
    36541 tatttccagt cgtagcgatt ggccatccag taaatcattc gttcgcccat cagttttgtc
    36601 tctgcataag ggttgacagg gtcgagcagg gtatcttcag tcaccggctt gtcaatacag
    36661 ttatttccac agagagaagc agtcgaagag aacatgattt tttgaatgcc aacttcagat
    36721 aagactttga gaacttggtt cataccagca acgttggtag tgaagtattt acttggattt
    36781 tcaatacttt cgctcacaac aatttcacct gcacaatgga ggacagcatc aatctgattt
    36841 tcttctaagt aagcctttaa ggcgctagca tcataaacat ccagttcttt aaagctagca
    36901 tgactatcca cagccgctcg atttcctgtg gagagattat cgaggacatg cacctgatag
    36961 ccagcattta aaagagcttt aacggtatgg gagccaatgt agctagtccc acctgtaaca
    37021 agaattgttt tggtcatgat tttttccttt tctagttttg tactatagta aattgaaata
    37081 agatatgaac aactctatta ggaaagtcaa attaatttct agaaatgttt cagcagctac
    37141 agcgtactat tccaaactca accaactata tatttaaaga aagaagaaag ggaagtcaac
    37201 tattttctgt aaatttcatg aaaaatccaa acaactttga tggtgtattg tttttttgtt
    37261 tggaaataaa tattcgagtt aattttggaa tgtttactag attttgcact acatgcaaga
    37321 gaatactatc cttttctaag atagagcagg actaagatac taacttagaa atccaccttc
    37381 ccactatcct tctcctataa aaagtggtaa aatggatgaa agaaagaagg aactgaaatg
    37441 acaacattat tttcaaaaat taaagaagta acagaacttg ctgcagtctc aggtcatgaa
    37501 gcgcctgtcc gtgcttatct tcgtgaaaag ttgacaccgc atgtggatga agtggtgaca
    37561 gatggcttgg gtggtatttt tggtatcaaa cattcagaag ctgtggatgc accgcgcgtc
    37621 ttggtcgctt ctcatatgga cgaagttggt tttatggtca gcgaaatcaa gccagatggt
    37681 accttccgtg tcgtagaaat cggtggctgg aaccccatgg tggttagcag ccaacgtttc
    37741 aaactcttga ctcgtgatgg tcatgaaatt cctgtgattt caggttctgt tcctccgcat
    37801 ttgactcgtg gaaagggggg accaaccatg ccagccattg ccgatatcgt ttttgatggt
    37861 ggttttgcgg acaaggctga ggcagaaagt tttggcatcc gtcctggtga taccatcgta
    37921 ccagatagtt ctgcaatttt gacagccaat gaaaaaaata tcatctcaaa agcttgggat
    37981 aaccgctacg gtgtcctcat ggtaagcgag ctagctgaag ctttatcagg tcaaaaactc
    38041 ggcaatgaac tctatctggg ttctaacgtc caagaagaag ttggtctgcg tggcgctcat
    38101 acctctacaa ccaagtttga cccagaagtc ttcctcgcag ttgattgctc accagcaggt
    38161 gatgtctacg gtggtcaagg caagattgga gatggaacct tgattcgttt ctatgatcca
    38221 ggtcacttgc ttctcccagg gatgaaggat ttccttttga caacggctga agaagctggt
    38281 atcaagtacc aatactactg tggtaaaggc ggaacagatg caggtgcagc tcatctgaaa
    38341 aatggtggtg tcccatcaac aactatcggt gtctgcgctc gttatatcca ttctcaccaa
    38401 accctctatg caatggatga cttcctagaa gcgcaagctt tcttacaagc cttggtgaag
    38461 aaattggatc gttcaacggt tgatttgatt aaacattatt aaaccataag ggaggtggta
    38521 gggatggcag aaccaaacct agaaagcctt ataaaagatc tttacaatca tgctcgacat
    38581 gatttgagtg aagatttagt tgctgctctc ctagagacta ctaaaaaact acctactaca
    38641 aatgagcaat tgcaggcagt tcgtctctca ggcctggtca atcgtgaatt gctcctaaat
    38701 cccaaacatc cagcacctga gttgctcaac ttggctcgct ttatcaaaag agaagaagcc
    38761 aagtacagag gaactgcgac ttctgcgctt atgtatgagg aactctttaa aatgctttga
    38821 ttccattgtg gatcaaagca ttttttgtaa gatctatctc gtgagcgttc aaatagtagt
    38881 aacaaggaat catgagaagt cagccgagcc catagtagtg aagaaacttc tgtaatggaa
    38941 gtggagcgaa gtgaggaatc gtcaaacggt aggggatagg ctgtttgaag ggtgctacga
    39001 aaaagatagg gtaaggtgaa attatagcgg aatgaaataa gatatgaaca aattgattga
    39061 ggaaattaaa tgaatttata gaaatctttt agcagtcatg gtgtcctatt ccagtttcga
    39121 aatgctataa atacgaacaa attgattggg aaattcaaat cgctttctat caatgtttta
    39181 gaagatacag tgctctagtc taacttcaat atactataaa agaacagatg gaagcaatta
    39241 tgatttactt ctatctttaa aataaatggt ctgataggaa aaatgtgtcc cctcataaaa
    39301 tataggatag tacgaatgga aggcttttgt agactggagg acgaaggagt ttagaaactc
    39361 tcgttaaaag ttagttgagc cggagtatgg tggtgtggat aattatcaaa aattatttct
    39421 aaatcgaaat agttgacagt tagaacgatg agtgttacaa ttgttttatt catttcgata
    39481 tcgaaataaa ttggaggtgt tatgaaagat ggtcatttgc tagcccatca tattcgtttg
    39541 ttgaatgggc ggatttttca aaagttactg agtcaagatc ctgaggctct ttataggggt
    39601 gaacagggca agattttagc ggttttatgg aatagtgaaa ctggctgcgc aactgcgaca
    39661 gatatcgcgc ttgcgactgg acttgcgaat aatacgctga cgactatgat aaaaaagcta
    39721 gaggaacaaa agcttgtaat tgttagtccg tgtggaaaag acaagcgtaa gaagtattta
    39781 gttttaacgg agttaggcaa gtcccagaaa gaagtggggc atcgtgtcag tcagaaattg
    39841 gatactatct tttacaaagg attttcagag gaagaaattc accaatttga aggttttcaa
    39901 gaaagaattt tggcgaatct gaaagagaag ggaaatgagg tttagaatat gtcacaacaa
    39961 gtgaaaaatg ctcacaacct atacatccat gccattcaag acggacgagt tgctgaggct
    40021 caagcccagt ctgtagggga tacctatatc caacattcga caggtgttcc tgacgggaaa
    40081 gaaggatttg cagccttctt tgcagatttc tttgagcgtc atccagagcg tcagattaag
    40141 attgtccgca ccattgagga tggcaatctg gtctttgttc atgttcatca atatctgaat
    40201 ggtggagaag ctcaatgggt gacgacggat actttccgtg cggatgagaa tggtcgtatt
    40261 gttgagcatt gggatgtcat tgactactat cgaacacctg aaaatgacca actagatcaa
    40321 atttttggag attttgaaat caaggatttg gacaagaaag cagaaaataa aaagctggtt
    40381 cgccgtttct tgacagaaat tttccaaaat ggggagctag agcagtggag tgattatgtg
    40441 gcagacgatt tgattcagca taatcatgat attggacaag gaagtgctgc ttataaaaac
    40501 tatgtggctg aatatagtgt cacttttgac tttgttttcc aactcttagg acaaggaaac
    40561 tatgtggtta gctatggtca gactcagatt gatggcgttg cttatgccca gtacgatatc
    40621 ttccgtttag agaacgggaa aattgtggag cattgggata ataaggaagt catgcctaag
    40681 gtagaagact tgaccaatcg agggaagttt taaattgagg acaaagaatg attgaataca
    40741 aaaatgtagc actgcgctac acagaaaagg atgtcttgag agatgtcaac ttacagattg
    40801 aggatgggga atttatggtt ttagtagggc cttctgggtc aggtaagacg accatgctca
    40861 agatgattaa ccgtcttttg gaaccaactg atggaaatat ttatatggat gggaagcgca
    40921 tcaaagacta tgatgagcgt gaacttcgtc tttctactgg ttatgtttta caggctattg
    40981 ctctttttcc aaatctaaca gttgcggaaa atattgctct cattcctgaa atgaaggggt
    41041 ggagcaagga agaaattacg aagaaaacag aagagctttt ggctaaggtt ggtttaccag
    41101 tagccgagta tgggcatcgc ttacctagtg aattatctgg tggagaacag caacgggtcg
    41161 gtattgtccg agctatgatt ggtcagccca agattttcct catggatgaa cccttttcgg
    41221 ccttggatgc tatttcgaga aaacagttgc aggttctgac aaaagaattg cataaagagt
    41281 ttgggatgac aacgattttt gtaacccatg atacggatga agccttgaag ttggcggacc
    41341 gtattgctgt cttgcaggat ggagaaattc gccaggtagc gaatcccgag acaattttaa
    41401 aagcgcctgc aacagacttt gtagcagact tgtttggagg tagtgttcat gactaattta
    41461 attgcaactt ttcaggatcg ttttagtgat tggttgacag ctctatctca acatttgcag
    41521 ttgtcacttt tgaccttgtt actagctatt ttgcttgcga ttcccttggc tgtttttctt
    41581 cgctatcatg agaagctggc cgactgggtc ttgcagattg caggtatttt ccagaccatc
    41641 ccgtctctgg ccttgttggg actctttatt cccttgatgg gaattgggac tttaccggca
    41701 ttgacagctc tagtgattta tgcgattttt ccgattttac aaaatactat tactgggctg
    41761 aagggaattg atccgagtct tcaagaggct gggattgcct ttgggatgac cagatgggag
    41821 cgtctcaaga agtttgaaat tccacttgcc atgcctgtta tcatgtctgg aattcggacg
    41881 gcagcggtct tgattatcgg tacggctacc ttggcgacct tgattggggc agggggactg
    41941 ggttccttta tcctgttggg aattgaccgt aataatgcca gtttgatttt gattggggcc
    42001 ctttcttctg cagtgctagc tattgccttt aacttcctac taaaagtaat ggaaaaagcg
    42061 aaattgcgga cgattttctc tggttttgcc ttggtggctt tattactggg tctgtcttat
    42121 agtccagccc ttttggctca aaaagagaag gaaaacgtga ttattgctgg gaaaataggc
    42181 ccagaaccag aaattttggc taatatgtat aaattgctga ttgaagaaaa taccagcatg
    42241 actgcgactg ttaaaccgaa ttttgggacg acaagtttcc tttatgaagc tctgaaaaaa
    42301 ggtgatattg acatttatcc tgaatttacc ggtacggtga ctgaaagttt gcttcaacca
    42361 tcacccaagg tgagtcatga gccagagcag gtttatcaag tggcgcgtga tggcattgcc
    42421 aagcaggatc atctagccta tctcaaacct atgtcttatc aaaataccta tgctgtagct
    42481 gttccgaaaa agattgctca agaatatggc ttgaagacca tttcggattt gaaaaaagtg
    42541 gaagggcagc tgaaggcagg ctttacgctt gagtttaacg accgtgaaga tggaaataag
    42601 ggcttgcaat caatgtatgg tctcaatctc aatgtggcga ctatgcagcc agctcttcgt
    42661 tatcaggcaa ttcagtcagg ggatattcaa atcacggatg cctattcgac tgatgcggaa
    42721 ttggagcgtt atgatttaca ggtcttggaa gatgacaagc aactcttccc accttatcaa
    42781 ggggctccac tcatgaaaga agctcttctc aagaaacacc cagagttgga aagagttctt
    42841 aatacattgg ctggtaagat tacagaaagc cagatgagcc agctcaacta ccaagtcggt
    42901 gttgaaggca agtcagcaaa gcaagtagcc aaggagtttc tccaagaaca aggtttgttg
    42961 aagaaatgat gggacagaga ctttaaacat actgaactca acttccttaa acgactagat
    43021 aaaagaatgc tcagacaatt tcgtctgggc ttt
//
LOCUS       DBEROU010000013        42536 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.013, whole genome shotgun sequence.
ACCESSION   DBEROU010000013 DBEROU010000000
VERSION     DBEROU010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 42536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42536
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.013"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(294..2021)
                     /gene="ezrA"
                     /locus_tag="ACL8CL_000753"
     CDS             complement(294..2021)
                     /gene="ezrA"
                     /locus_tag="ACL8CL_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HGS1330038.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
     gene            complement(2103..4049)
                     /gene="gyrB"
                     /locus_tag="ACL8CL_000754"
     CDS             complement(2103..4049)
                     /gene="gyrB"
                     /locus_tag="ACL8CL_000754"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HGS1330039.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFASHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            complement(4064..4627)
                     /locus_tag="ACL8CL_000755"
     CDS             complement(4064..4627)
                     /locus_tag="ACL8CL_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1330040.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQT
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDILNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            complement(4712..5266)
                     /locus_tag="ACL8CL_000756"
     CDS             complement(4712..5266)
                     /locus_tag="ACL8CL_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1 family glyoxalase III"
                     /protein_id="HGS1330041.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            complement(5277..6500)
                     /locus_tag="ACL8CL_000757"
     CDS             complement(5277..6500)
                     /locus_tag="ACL8CL_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGS1330042.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVSLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            complement(6537..8987)
                     /locus_tag="ACL8CL_000758"
     CDS             complement(6537..8987)
                     /locus_tag="ACL8CL_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HGS1330043.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDVHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTAASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEGFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSCRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPFVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIIGKRYGKQIVASLAEEATIK
                     TISRSEVDEAIDRFFNEL"
     gene            complement(9187..10116)
                     /locus_tag="ACL8CL_000759"
     CDS             complement(9187..10116)
                     /locus_tag="ACL8CL_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HGS1330044.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELLVAVNALMHQDMMDRIKPFLDFLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARNWIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            10256..10543
                     /locus_tag="ACL8CL_000760"
     CDS             10256..10543
                     /locus_tag="ACL8CL_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HGS1330045.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRR"
     gene            complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="ACL8CL_000761"
     CDS             complement(10577..11911)
                     /gene="ciaH"
                     /locus_tag="ACL8CL_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HGS1330046.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="ACL8CL_000762"
     CDS             complement(11901..12575)
                     /gene="ciaR"
                     /locus_tag="ACL8CL_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HGS1330047.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            complement(12684..15230)
                     /locus_tag="ACL8CL_000763"
     CDS             complement(12684..15230)
                     /locus_tag="ACL8CL_000763"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HGS1330048.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLSLRFDQAEGELALSNMP
                     EIDVENRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            complement(15353..15793)
                     /locus_tag="ACL8CL_000764"
     CDS             complement(15353..15793)
                     /locus_tag="ACL8CL_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HGS1330049.1"
                     /translation="MIPQEMWNKYKQINPLIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQSVCIVEITKVSVELFNQVSAQHAFKEGE
                     GDKSLAYWRQAHEDFFTDCLGEVGLTFTPESKVVLEEFRKVYPL"
     gene            complement(15768..16637)
                     /locus_tag="ACL8CL_000765"
     CDS             complement(15768..16637)
                     /locus_tag="ACL8CL_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HGS1330050.1"
                     /translation="MRNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNIILAS
                     CQQFVKDYLDTICSFYLGKEVWYRFSELTNTEANCLVGTREFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPSVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDMESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            complement(16679..17407)
                     /locus_tag="ACL8CL_000766"
     CDS             complement(16679..17407)
                     /locus_tag="ACL8CL_000766"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1330051.1"
                     /translation="MELFKTWKKNMVLYGLKSQIGTVYRNNDRTTSFYDVGNFLYLAG
                     ELDSRFWEDFVRKYGLDYKIIISENTNWQDFLHRKVGLNSFTRYSFKDKANFQVEFLN
                     NLVTHLEEGYNIVPIDNHIYNCFSTEEWSQDLQGDFESYQDFVLKGGFGFVILKNNEL
                     IAGISSGLVYRKAVEVEVATRPNEQGNGLAKKLGAAMILESLNRDMFPLWDAHNEASK
                     KVAEFLGYELSEPYEAFELEEILI"
     gene            complement(17415..17894)
                     /locus_tag="ACL8CL_000767"
     CDS             complement(17415..17894)
                     /locus_tag="ACL8CL_000767"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS1330052.1"
                     /translation="MEIKKHFGVYAVCFENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELTNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVK"
     gene            complement(17905..18603)
                     /locus_tag="ACL8CL_000768"
     CDS             complement(17905..18603)
                     /locus_tag="ACL8CL_000768"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HGS1330053.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKPLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQEIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            complement(18596..18832)
                     /locus_tag="ACL8CL_000769"
     CDS             complement(18596..18832)
                     /locus_tag="ACL8CL_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018367459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HGS1330054.1"
                     /translation="MTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTIEQNGLALEMT
                     PYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            complement(18928..19854)
                     /locus_tag="ACL8CL_000770"
     CDS             complement(18928..19854)
                     /locus_tag="ACL8CL_000770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HGS1330055.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEEFVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSVAFTPGFESAFHVNMEDSQAAMRDGSIFEYCKLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSQAANFSLTRKEWYEIYLAAGNNLP"
     gene            complement(19929..20744)
                     /locus_tag="ACL8CL_000771"
     CDS             complement(19929..20744)
                     /locus_tag="ACL8CL_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330056.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKFGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPDPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFLLFHGFKKIKWSEV"
     gene            complement(20748..21068)
                     /locus_tag="ACL8CL_000772"
     CDS             complement(20748..21068)
                     /locus_tag="ACL8CL_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGS1330057.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            complement(21158..21394)
                     /locus_tag="ACL8CL_000773"
     CDS             complement(21158..21394)
                     /locus_tag="ACL8CL_000773"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330058.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDRTDEVSSKHVFEVVDETDEVS
                     SKHVFEVVDETDEVSNHTYGRATLTWFEEIFEEY"
     gene            complement(21444..23441)
                     /gene="metG"
                     /locus_tag="ACL8CL_000774"
     CDS             complement(21444..23441)
                     /gene="metG"
                     /locus_tag="ACL8CL_000774"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HGS1330059.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLAKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            complement(23565..24824)
                     /locus_tag="ACL8CL_000775"
     CDS             complement(23565..24824)
                     /locus_tag="ACL8CL_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1330060.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIASIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESL
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            complement(24826..25527)
                     /locus_tag="ACL8CL_000776"
     CDS             complement(24826..25527)
                     /locus_tag="ACL8CL_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330061.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            complement(25511..26710)
                     /locus_tag="ACL8CL_000777"
     CDS             complement(25511..26710)
                     /locus_tag="ACL8CL_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HGS1330062.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAQAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            26996..28342
                     /gene="gor"
                     /locus_tag="ACL8CL_000778"
     CDS             26996..28342
                     /gene="gor"
                     /locus_tag="ACL8CL_000778"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HGS1330063.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNREAYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDDVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGLYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSSCTRNRQESRFKLI
                     TAGSEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTASEEFVTMR"
     gene            28539..29075
                     /locus_tag="ACL8CL_000779"
     CDS             28539..29075
                     /locus_tag="ACL8CL_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HGS1330064.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            29136..29282
                     /locus_tag="ACL8CL_000780"
     CDS             29136..29282
                     /locus_tag="ACL8CL_000780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330065.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDETDEVSNHTYGKAKLMRFEEI
                     FEEY"
     gene            complement(29279..29494)
                     /locus_tag="ACL8CL_000781"
     CDS             complement(29279..29494)
                     /locus_tag="ACL8CL_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HGS1330066.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGASAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            29592..29912
                     /locus_tag="ACL8CL_000782"
     CDS             29592..29912
                     /locus_tag="ACL8CL_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330067.1"
                     /translation="MRHKFQQVLNKIHDFLNGYDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFSKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            complement(29943..30281)
                     /locus_tag="ACL8CL_000783"
     CDS             complement(29943..30281)
                     /locus_tag="ACL8CL_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HGS1330068.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(30293..31012)
                     /gene="trmD"
                     /locus_tag="ACL8CL_000784"
     CDS             complement(30293..31012)
                     /gene="trmD"
                     /locus_tag="ACL8CL_000784"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HGS1330069.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDEPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            complement(31002..31520)
                     /gene="rimM"
                     /locus_tag="ACL8CL_000785"
     CDS             complement(31002..31520)
                     /gene="rimM"
                     /locus_tag="ACL8CL_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HGS1330070.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            complement(31590..32338)
                     /locus_tag="ACL8CL_000786"
                     /pseudo
     CDS             complement(31590..32338)
                     /locus_tag="ACL8CL_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819199.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
     gene            complement(32442..32766)
                     /locus_tag="ACL8CL_000787"
                     /pseudo
     CDS             complement(32442..32766)
                     /locus_tag="ACL8CL_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654120.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
     gene            complement(32915..33154)
                     /gene="kphA"
                     /locus_tag="ACL8CL_000788"
     CDS             complement(32915..33154)
                     /gene="kphA"
                     /locus_tag="ACL8CL_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein KphA"
                     /protein_id="HGS1330071.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            complement(33174..33446)
                     /gene="rpsP"
                     /locus_tag="ACL8CL_000789"
     CDS             complement(33174..33446)
                     /gene="rpsP"
                     /locus_tag="ACL8CL_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HGS1330072.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            33616..33825
                     /locus_tag="ACL8CL_000790"
     CDS             33616..33825
                     /locus_tag="ACL8CL_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330073.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LADDSLAGDIILLWRIQFGTFTTET"
     gene            34304..34525
                     /locus_tag="ACL8CL_000791"
     CDS             34304..34525
                     /locus_tag="ACL8CL_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330074.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEEVEDFQDDDEIPTII"
     gene            34609..35412
                     /locus_tag="ACL8CL_000792"
     CDS             34609..35412
                     /locus_tag="ACL8CL_000792"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HGS1330075.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIGGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(35469..37010)
                     /locus_tag="ACL8CL_000793"
     CDS             complement(35469..37010)
                     /locus_tag="ACL8CL_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS1330076.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHELDFYEGWIDQIWD
                     FNNLT"
     gene            complement(37012..37494)
                     /locus_tag="ACL8CL_000794"
     CDS             complement(37012..37494)
                     /locus_tag="ACL8CL_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGS1330077.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPGIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            complement(37494..38579)
                     /gene="rlmN"
                     /locus_tag="ACL8CL_000795"
     CDS             complement(37494..38579)
                     /gene="rlmN"
                     /locus_tag="ACL8CL_000795"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HGS1330078.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERISHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSTSGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRGVTFEYIMLNEVNDGVEQALE
                     LTELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            complement(38600..39130)
                     /locus_tag="ACL8CL_000796"
     CDS             complement(38600..39130)
                     /locus_tag="ACL8CL_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HGS1330079.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKGQRHFVIRQK"
     gene            complement(39286..39891)
                     /gene="sodA"
                     /locus_tag="ACL8CL_000797"
     CDS             complement(39286..39891)
                     /gene="sodA"
                     /locus_tag="ACL8CL_000797"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase SodA"
                     /protein_id="HGS1330080.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            complement(40064..41101)
                     /gene="holA"
                     /locus_tag="ACL8CL_000798"
     CDS             complement(40064..41101)
                     /gene="holA"
                     /locus_tag="ACL8CL_000798"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HGS1330081.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQMLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKADSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            complement(41135..42070)
                     /locus_tag="ACL8CL_000799"
     CDS             complement(41135..42070)
                     /locus_tag="ACL8CL_000799"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HGS1330082.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
BASE COUNT        12663 a         9270 c         7623 g        12980 t
ORIGIN      
        1 aagttactca aagtacagat ttgaagctgc agataagact gacgaaatca ctttaatact
       61 aaatgaaaat caaaaagcaa actaagaagc tagcctcagg ctgtacttga gtacggtaag
      121 gcgacgctga cgtgatttga agagattttc gaagagtata aaacactgtt ttgaggtggt
      181 ggataaatcg gctgggtaaa aactcaatct caggagaaaa tgaagtaaat ctccccacaa
      241 ctatagaaaa agatttgatt ctgctcctca cacaataaaa tctttttctt ttattaaaaa
      301 cgaatcgttt cacgtgtttt ctcatatgag gtaacaaagc gattggttac accaggctct
      361 gccacttcca atgcttgaga aatcttgtca aatgaagcgt gataatcaaa ttctttttca
      421 aaaatatcta aagcttcgtt aaatgcttct tgaatgcgtt catcaaatga gcgatagcgg
      481 ttagaatatt gcaagagttg ctctgtcaaa gttgcatatt gtacaatatt ataagtttcc
      541 gtttctaaag cttccatatc attcgttgca atttcaagaa ctcgggtaac agattcaatg
      601 ttaatcattt tttgttctaa ctcaaccatt aaatcctcgg tattattgct tgccgtaaag
      661 aataacttca agaaagtttg tggaatacct ggcagattgc gtttttccat gtatcgcttg
      721 atagtatgga gacgattgac ataaacattg gccttttgac gtgcattaat atcatctttc
      781 tcaatttgtg tcaggcgctc actaactgaa atttgctcat cttcaatatc ttttagttga
      841 gtttgtaaat cctcaagatt ttcttcaaga actgaataag cttgggttgg ttcttcttga
      901 tttgaagtta cctcaacaat agctgcctca aaactctcta attctgtctg aatacgacga
      961 acatggcttg cagctgtctc aggaagtaaa taggtcttgt tcaaacgtgc aatatcttct
     1021 cccaataaag tattattctc tttcatatgt tgaaggtaag ttggaagagt tgcaagtaga
     1081 ttttccacta ctttctgagc agcaatttct cgagtaaaaa tatcatacaa ggcattgatt
     1141 tcctcttgtg cctgtccatt ctcatattcg gcattatcca attccaactg acgaatattc
     1201 tcttggtttt tcttgaatgc ttcataaagc aagtggaaac gcgcttcaat atccgtttca
     1261 acaaaatgat aattagcatc aattagttta cgataaccgg attccaaatc ctgtaattga
     1321 tctggcaatt ctgtagaaag cgtcgtaacc aaggctggaa cacgatccac aatatgactt
     1381 aaggccaaaa tgtgattttc tgtattatcc aaaatcactg cggcttccac agggtcaccc
     1441 gatgaattca aggttacaaa ttgtgaaaat tcagattgga tattttctaa ttgtttttca
     1501 atttcatcca aggcttgacc atactgttct gaattttcag caactctatg ctgaagttcc
     1561 tcaaataaat ccaaagcatg aagaacacga ccactatttt tagattcttg cttctctaag
     1621 tctgccaaag cattgcgaat tgccgcaata tcttcttcaa tcaaagtaat ttgactctca
     1681 atttggtcaa tttgatgact ggccttgaga aaacgaaatg aatggttata accttctgct
     1741 tcaaagagat tattttcaat atcggcaaaa gagttgagag ataaatcgac ccatttttga
     1801 ttccattcac ggaaagccac ttgactttgt ccaatcaagt gcatattttt tacagcttct
     1861 acttcatcat ttactggaag attgtatagt tcttcttttc tttcttctag cgcctctaat
     1921 ctcccctcgt ttcgcttacg tagaaagatt gccactacat aagccagaac taaaatgact
     1981 gcaattgcaa ccattaaata aattagttgt ccattagaca tatcaaactc cttttttact
     2041 tgaaacaatc gtaacgatta tatcatattt tttagaccaa gggaactact tctccctatt
     2101 ttttagacat caagtgtact atagacagca ttttcttcga taaactcacg acgaggctct
     2161 actcgatccc ccatcaacat atcaaagatt ttatctgctt ctgcagcatc atctacagaa
     2221 actctagcca tcaagcgatg ttcgggatcc atggttgttt cccacagctg atgatcgtcc
     2281 atttcaccta gccccttata acgctgaata gtcggtttgg tacgaccttc actataacgg
     2341 gctaaagctt cttggagttt gatttcttga tctgcacccg gctggatata ttctttaatc
     2401 tcgctcccaa ccttgacacc atagattggt ggttgggcaa tataaacata accagcttct
     2461 aggattggtt tcatataacg ataaatcaag gttaaaagaa gggtacgaat atgggctcca
     2521 tcgacatcgg catcggtcat caaaacgagt ttttggtaac gggctttcga aacatcaaat
     2581 tctgcgccaa atcctgttcc catggctgtg aaaagactac gaatttcttc gttagctaga
     2641 atcttatcca tacttgcttt ttcaacgttc aaaatcttac cgcgaattgg aaggatagcc
     2701 tgaaactcac ggttacgacc agatttggct gatccaccag ctgagtctcc ttcgacgatg
     2761 aagagttctg tttcagcagg gttattagaa gaacagtctg ctagtttccc tggaaggttg
     2821 gaaatttcca aaccagattt tttacgtgtg acttcacgcg cacgcttggc agccacacga
     2881 gccttggcag ctaaaatccc tttttccacg atacgtttgg caatctgtgg attttccatg
     2941 aggaaatcgg agaaggcttc actgaagagg cgattggtaa tcttgaccac ttcgctattt
     3001 cccaatttgg tcttggtttg tccttcaaac tgtggatttg ggtgtttaac tgagataact
     3061 gcagttaatc cttcgcgaac atcttcccct gttaaattat cttcattgtc tttcagtaac
     3121 ttattcttac gagcataatc attgataaca cgagtcagtg ccgtacggaa accttgttca
     3181 tgcgttccac cttcatgggt atgaatatta ttggcgaaac tcatgacatt ttcatggtaa
     3241 cctgttgtgt actgcatggc tacctcaact gtaatatcat ccatctcacc gtctgtatag
     3301 attggtgtat caaagattac atccttgttc tcgttgatat attcaacgta actagcaatc
     3361 ccaccttcat aatgataatg cttggtttgt tccaaacctt ggcgcttatc agtgatagaa
     3421 atttgaagac cgcgatttag aaaggccaac tcttgaatcc gtttatttaa tttatcaaaa
     3481 tcaaaggttg ttgtttcagt aaagattttt gggtccggta taaagtgaac cgttgttcca
     3541 gttctatctg tatccccaac cacctcaagg tctgcaacaa catgaccacg acggtattct
     3601 tggtaatgaa tcttaccgtt tttatggaca tgaacgtcta attgagtgga aagggcatta
     3661 actactgacg accccacccc gtgaagacca cctgaaacct tgtatccacc accgccgaac
     3721 tttcctccag catgaaggac tgtaaagacg gtctcaacag caggacggcc tgttttttcc
     3781 tgaatatcga ctgggatacc acgcccatca tccacaacag taatcgaatc atctggctca
     3841 ataaaaactt gaatatggct ggcaaatcct gccaaggcct cgtcaattga gttatcaaca
     3901 atttcccaga ctagatggtg aagaccttct tttgaggttg atccaatgta catccctgga
     3961 cgcatacgaa cagcctctaa gccctctaaa acttgaattt gactggcatc ataatcctgt
     4021 gcctgcagat ttttgatttc ttctgtcatc ttattccttt ttcttatatg tctaatactt
     4081 gtcttaaatt cacgatactg gtaaacaagt gggtatcaag tcctgcagct tgacctgctt
     4141 cgatatcaat cggccgatca ccaatgacaa gaccagagct aatctgatac ttttctctta
     4201 aataaagcat ggattcggga tttggctttc tcttaaagcc tgagctagaa gtcaccactt
     4261 ctgtaaaata agctgctata gaggtttttt ctaaaatttc caaaacctga tcatttcgat
     4321 gagagaccaa aaaatgacgg ccaccttgat ttaaaatgtc ttccaatagg tcagaaactc
     4381 cttcaaataa aatcgggtgt tcaagctctc tggcttcatt ttccttgtac ttttctaaaa
     4441 aattctctaa attgggagcg aatgtctcaa tcgcaaaagg agtagaaacc tttaaagttt
     4501 gatagacact gtcatggtct tgtgtgatac catacagtgc caatgtttca acaaatgcag
     4561 ctgttgaagt ttcataatta tccagtaaag ttccacctaa atcccagatg taatcgtgat
     4621 atttcatacc cttcattata gcattttttt atgaaaaaag cgatgatttc cctgagtttt
     4681 ccacataaaa aacgagagaa taactcccgt tttactgatt tttaccaaag acatctcgat
     4741 agagcattcc tgttcgtaaa ctctctgcgt cccctcctag ttgctccacc aactcgtagg
     4801 caaaggcaag ggctgttgaa ggaccccgac tggttgtcaa ctgaccatct accactactg
     4861 tttccttgac gtagtgacca tcaaggattt gctcttgaac gccgtcataa caagtgtatc
     4921 gcttattttt caatatctct gcttgattga gggcaattgg tgccgcacaa atggctgcta
     4981 gtttcttccc ttcttgttcg aagctttgca attcttgaat caaggtctga ttatcacgta
     5041 aatgtgcaga accaggcata cctccaggaa gaacaatcat atcatagtct gataaatctc
     5101 catcaaagac atgatctgct cttacttgga ttgcatgcga acccgttact tgctcttcaa
     5161 aaccaaccat atcacatgtg atattggctc gacgcaagac atctacaact gtcaaggctt
     5221 caatttcttc aaagccctga gctaacataa ctgctacttt taccatgatt ttctccttat
     5281 ttaatttgtt ttaatacaac ctttttccgt ttgaatggga cttttccgct cttttcttca
     5341 gctagattag tctggtaact catcgataaa agcaaaccaa caccaatcag attactgata
     5401 atagctgatc ccccttgcga aatgaaaggc aagggaatcc ccgtcaaagg aagtagtcca
     5461 gtcacagcac cgatattctc aaagatgtgg aagagcaaca tcataatcaa acctgtggaa
     5521 atataagtgt agaactggtt atttgattta agagtaatct tcaacatacg gtaaatcaac
     5581 atgagataga gggcaataac caggacagag ccaataaagc caaaatcttc tgcaataacc
     5641 gtaaaaatca tatctgactc tcgaactggg ataagcagat tcgaagcatt aaatccctga
     5701 ccaaataagc caccactccc aatggcaatc tgcccttgag cctgctggta agtcgttgtt
     5761 tgggcaaact caaagggatt gagccaagcc aaaatccgat tgatttggta ggtcggcatt
     5821 ccaatctggt gaagaaaagc tcgtccgtcc ttgctaataa agatagctaa gaaaccagca
     5881 actcctgtta cagcagtcac aaatactggg ataataattt tccaagaaac ccctgataat
     5941 aaaacgattc ctgagaaaat ggctacaaaa accaaagccg tccccaagtc actttgaagt
     6001 gctaaaagaa ctaggactgg aatggtaaag agaatcatcc agaaaattaa caaaaagtcc
     6061 agcgaaaccg tgcgtctcca ttccttatgt ttctttgtaa attggacaat gacacgagcc
     6121 aacatgagga tataggatat cttcataaat tctgacggtt gaaatagggt aattccattt
     6181 attgataccc agtttttggc acccgttgat gcaactaagc ttggattata aaatacaatc
     6241 ggcaagatca taagtcccaa gcctaaaata tatagaaagg gggtcacctt ccaaagaaat
     6301 tctgtattaa agagcatgac cacaaaacca atcacaagcc ccaaggcaat ccaggcgacc
     6361 tgctgcccta aaatgggcag aatattattg ggataatcat gactaacggc tatatagata
     6421 gccaccacac cgatgaccag tagaaaaaat actggcaaga gcaaactata atcaactcta
     6481 gagtcgagag aacgtttcat atatactaac cttatacttt catacaatac tatttatcaa
     6541 agttcattaa aaaatctatc aatagcctcg tcaacttcgg atcgagagat ggttttaata
     6601 gtcgcttctt ctgctagaga tgctactatt tgtttgccgt atcgttttcc gatgattctc
     6661 ctatccaaaa taagagttaa ggaacgttgg tattcacgtc tcatacttct tcccaaagcc
     6721 tgttttaaac gaataatggc cattggcaat tgataatcat aaaaggcatt tttcccttct
     6781 tgattcagtt cttgattaat ctttttcgtc aagggttctt gaggattttg gaaaggaagc
     6841 ctcggtacaa cttgaatcac aaaaggatgg cttgaaaaat caactccctc ccagaaactt
     6901 gctgcaccaa gcaagatttg ttgttcacct ttttcaaagc gtttctttag ctgatgaaca
     6961 tccccatttt tatactgggc caagtggcta actgtaagta aatccgatac tgctagaagc
     7021 atgtctttag cggtaaagag aaccaaaatc ggttgctgga aagcttgaat ttccactagt
     7081 aaagcagcta cctctctggc atagacttct aaggaggttt ctgttaccag agggaaatct
     7141 ttgaccataa ccacttcttg ttcctgtttt ccccgagatt caatcttgac aaatttggct
     7201 tcaggatagc ctaaaaggtc tgccaaagaa accctctgac taatctcaag agtagccgat
     7261 actcccaaga cttggcaact ctcaggaagt aaagaggaca gaagagtacg gcatgatttt
     7321 gtagaagaaa tctgaatttt cttttgactc gtttcagtta cttcaagcca gtaatcacct
     7381 tcagctgtaa aatagcgaac cagctcatca aagccttcta cttctaattc tgaaaaatac
     7441 tgatggagat tgtccagaga atctaagata tttttcctag atttaccaga ctgaaattgt
     7501 tctatcaagt agagacactc aaagcgaata ctttctagta tccgttgttg aaccctgttt
     7561 tcttctccta ctaaagcctt atcaattaaa tcgataatag attgtatatc gtaggtctct
     7621 tgaagcagat tttctagagc taacaaaatc ttttggactt catcaataat cagtaaacgg
     7681 tcactgacaa attcaggatt atcttcaagt ctggtcacga gataggcatg attagtcact
     7741 aaaagcttgc aagtctctgc cctttcttga ctacgtttcc aaaaatcttc cgtcacaaat
     7801 aagctctggg atgataaatt cccatcatga cgaaggtctg ctagaaaatg ttggtaacgg
     7861 tagagttgcc cgatttcatc caaatctcct gtctctgtct cagtaagcca gaccaagact
     7921 tgcattttaa agcgtctaaa taagcgattt tcatcatttt cctgcaagga atgataaaag
     7981 gcatccaact tcagataatt ttgtggtccc tttaagctat gaatatctgt atggaacact
     8041 tccttgaggc gtttaccttc ttcttccatg atttgatttt gaagaatctt tgtcggaaca
     8101 ctaagaacaa tttgtcgctc tttggattga gataaagcgg gtaagagata gccataggtt
     8161 ttcccaatcc ctgtcggcgc ttgaatcaga gagacaggtt catctttcaa tagcaagcca
     8221 acctctttag caaaactttc ttgttcctcc ctcacttcaa ggttcaacag agaaatattt
     8281 ttagaaaagt cttgagatag ttttcgtggc tccagagaag ctgccgtttt cttaaaatac
     8341 agaccttgaa cttggaccaa gtctggagaa ctcaggatag attggttgcg ataagtttcc
     8401 tcaataacca ggtaggactc atataggaga gcgtcagcca tttccagcaa gcgttccaag
     8461 agacctttag gaagctgagt catcttttcc cgtaaaaaaa gaagtaattc tgctgtagct
     8521 tgggcatctg aaagggctgt gtgtgcgtgt ttaagaggaa ttcctaattc tcgacacaaa
     8581 atcggcaagc tatatttttc cagttcaggg aaaaagacct gggccaattc gaccgtatca
     8641 acacgagggt ttcttagctc atagccttca aaaaataaat tttccgccaa gagattagca
     8701 tcaaactgaa cattatgggc tacaaaaatc ccatcctcca ccaagtcaaa tatttttctg
     8761 gcaacttgcg aaaaatcagg tgcttgcgcc agacgttggt ctgtcaatcc tgtcagttct
     8821 ttgatatgaa catccaaggg ttcatgtgga ttgacatccg tcgtatagtg atcgacgatt
     8881 tctccgtcct caatcacgac aattcccact tggataattt tagccttact acctgtgcta
     8941 gttgcctcta aatccaccac aacatagcga ttaccaatcg ttttcattat aaaaaattat
     9001 accaaaaaaa gggccatttg gcacctgcaa acataggata attttcaaac tcaagaacac
     9061 tacttttctt aaaaatccca tcgaatagag cattctagcc tgctgatttt tgtaaaatag
     9121 tgaagaaata cgaaaaattg agagaagaaa tgtttgcatc ttccttctct caacgaatca
     9181 tgtattttat ctaaccatgt caggatcgta gtcataaaat cctgtatcaa ggaaacggtt
     9241 tttagggtga agtttacgaa cttcttcatc cagcaagaag gcttgatcat ggctaaagtt
     9301 gccctcttga atccaattac gcgcttggat aaagagtttt gcaatctcaa caaagttctg
     9361 accaggagtg tagagccctt ctagtttcca gcgagtaaag ccatgctcca ccaattctgt
     9421 taatttgatc atcaaatcaa ggtcattgtt ggcaaagata tgggtcccat gattatcttc
     9481 aaaaatggaa tagtggctct ctggatcact tggctcagcc aagaagaggt cacgtttatg
     9541 cgtcttttca tcatcgatat gtgtaaagtt atagtagttt tgcaagagtg gacgtttaga
     9601 atgatggatg acgctagcac cgtaaaccaa aacttcagca ggaatttcca aaatctctgg
     9661 cattttgaaa agttcagctg atggaatttc acgcgccaaa acagcctcag atgcgccagc
     9721 cttttgtccc cagaagttaa tctgacgact gctagttacc atggttgaag catcgtagat
     9781 ggtcttaaat gaataaccat cgcggttaac tacgtaaaag acgcctgcat ccccaatcgt
     9841 aatatagtct gtcttgattt cttccaagaa gtctaagaaa ggcttgatac ggtccatcat
     9901 atcttggtgc atgagagcat tgaccgcaac gagcaattcc ttaccagcat catgaaccaa
     9961 cttagcgatt tcacgtaatt ggtcataact aaaggtcgtt ggcagacgaa ggccaaaatc
    10021 tttctcaccg acatagatac ggtctacgcc agcttcgagt agttgttcaa cttgttcaat
    10081 actttcagca gttgctgtaa tgacaatctt ttccataaga aaaattatac cacatttctc
    10141 caaaatcagc catgctttga aaatgaaaag ggactttatt ctttttgtaa cctttctgta
    10201 ataattctct gccgaaaaaa tgataaaata ggtatgtact gttaaggaga gaattatgcc
    10261 cgtaagaaaa ttacaatcct atgaggtaga ctatcaagaa gaattaaacc agcagcttcc
    10321 tcattaccaa gcttatacac ctgaagcgca atctgatgcc aatctcaagg aaatcctatt
    10381 ttttattaat atcgctgttt tttgtatctg tattgctatc tttagtttta tctttttagc
    10441 attaaaatta tcaactgctc ttgcctttgc cgcagcaatc ggattcagct tacttgtttt
    10501 aaaagttcaa cggtctatta tcaaacgaaa acgtagaaga taaatccaaa atatcgcccc
    10561 agttggagcg atatttttat tttttctttt tagatggtgt ctgaatggca atcttcactt
    10621 caaagattgt tcccttgggt ttattatctt tgacagtaac agttcctttt agagcatcta
    10681 caatttgctt ggctagggat aatcctaaac caaaaccacc tttttgccga gttctagcct
    10741 tgtctactcg ataaaaacgg tcaaaaattt tctttttatc ttctgtcgaa ataccgattc
    10801 cattatcaga aacaagtaaa taaagattgc gatcggtcgc cgagataaga aaatcaattt
    10861 caccatcctc ctcagtatac ttgacggcat tatcgaaaag aatggtcatc agttgtttca
    10921 gaagaagctg atctgtgaca attgttcgat ggatacgatt ttcaaaacgg aagacacgat
    10981 tattttccga agcaatcatc tcgtagtttg tgaaagttgt attaaaaaag ctagttggaa
    11041 cttctgcaag ctccggctta atcccatcat ctctccgagc taagttcagc aagcttgtcg
    11101 ttaaaaaacg catatttcgg acttcttcca aactcgatgc aatgctttcg ctcacatcca
    11161 taatggtagc ttctggctta cgaaaaaggg tctctaagcg attttgcaaa actgcgagtg
    11221 gagttcgtaa ctcatgactg gcattttcca caaaagactg ttgcttctgc atactctcaa
    11281 gcaggggcct aacactgacc ctagctagat agagactggc aagtaaagac aaaatccaga
    11341 aactagccat cacgaccaca atcaattgct catgcttttg actagcctgc tccaactgac
    11401 tggtattaat caagacagca gcatacttga tattggttga aaccgaacta atattggtct
    11461 ccatcaaaat cacacgatag atttcttcct gcccatagct attaaaaacc tgaatctggt
    11521 agatatgtcc tagttctttc ttttctaact taatcttatc caagcccaaa aatcgatttc
    11581 cagaaagaag ttgagtaaag tccttatcaa agagaatgac ttccgtattg gaactgacat
    11641 taggttttat ttctacttta ctagcgtccg ccctagcatt ttctaaatct ttaatctctt
    11701 ctgttgccct atttatagcc agctgaataa ctgcttgagg attttcactc agtccatgaa
    11761 gcttatcgtc caccgaagta taaagactcg aatgcatgac ttgtaaaata atcagagtca
    11821 ttgtagaaaa aatcagggtg aagacaccga agttgcggat aaaataacta aagtcatccg
    11881 cataccatgt ttttttaagt ttactgaaca tcttttaaaa gatacccaac actacgcaag
    11941 gtttgcaaat tctctgcaaa agtggttccc tttaatttct tacggacttt tgaaacatag
    12001 acttcgacaa ccgaaatcgt tgtatcacta tcaaatcccc atagacggtc aaaaatctgc
    12061 gtcttaggca aaatcacatt ttgattttga aggaaataaa ctagtaaatc gaactctttc
    12121 cccagcaatt cgacaggagt atcttcaact ttaacggtat tggttgataa attaaccacg
    12181 atattcccat aagtcaaggt gttttcatta aacttccctg aacgtttgag aagggcctga
    12241 atccgcattt taagttcttc taggtagaaa ggtttggtca gataatcatc cgctcccagt
    12301 tcaaatccat gtcccttgtc atccaaactt tccttggcag tcataatcag aactggtgtc
    12361 gtaattccct tttcacgcaa ttcttttaag acttggaaac catttttttc tggcaacatc
    12421 aaatccagca aaatcaaatc atagacacca ctctcagctt cgtagagacc ttcttctcca
    12481 tcaaatacct gcataacatc cgcaaaatcg tctaaaaagt caaatactga atttgacaga
    12541 cctaggtcat cctcaaccaa taagattttt atcatgagaa actcctcctt attaaaacta
    12601 ttataccaaa tttgccttaa aaaaaactca actctctgca ttttacatga gatagctgag
    12661 ttttcttttt attttaggct tatttatgca tttccgtatt gaagaacaac tgcttcgact
    12721 gcagcttttt cacggctaat caagtcaaca cgtgctgcaa tttccttgat tcccataccg
    12781 atgttacggc taagagcaag gtcagaaagt tgcggctcaa agaactcctt gtattccgcc
    12841 aagcgttgct gagtcttaaa tacatgagca ggaaggataa caaagctatc aaagctcata
    12901 tctcctccaa gggctgcctt aatccaagcc cagttttcac gcgcccaaga ccaagctgtt
    12961 ttctgagttg cttgatgagc taggaattgg taataccaag cagacaagtc ctgtggtttg
    13021 accacaaatt tgtccttcca agaagtaatc aagttttgga tattatccgc atctgtactg
    13081 tatgcaagag ctgctgccaa ctggcgttta aagacagcat ctgttgcgtg agtataagta
    13141 tcaagataaa gtgctaacaa gtctttagtc tcatgatgtt tcatctcatt aatcagaact
    13201 tgtgaacgaa tagctgctgg gagtcctgca agattctcct tgtgtgttgc gaagatttgg
    13261 ctagcgactt gactagcttc tgcatcattt gagcgaatca tcatcgaaac agccagctga
    13321 cgaaccaatt catcctcatc tgattctccg tctttagctt caaaaccaag acggtcatag
    13381 ttatgacgag ccaatttagc aaccagtcct ttgaaggctg tttcagcatc cgttccttca
    13441 tcaataaagc gctcaagggc tgaaatcact tgagaaacag ctgaaaccac cagataagac
    13501 tcttccttag caagtttatc aagaactgga agcaagtctg cataagaaat gtgccctgcc
    13561 tcagccaaca aacgacgttc ttgaacaatt tgcagtttgc ttgtgttatc aagtgtctct
    13621 agctcagcaa gaacagcttc taacaagtct ccttgatagt cggtaatata gtgggcagta
    13681 ttttcagtgt tgagacgaag agctccttca ttttcagcaa gaagagctgc gtagccaggg
    13741 atttcgatac tttcagtttc gagtgtatca ggcaagcctt tccagttgct attgaggggc
    13801 accacccaga gacggttctt gtcttcgttc tcaccgatga agaattgttt ttgtgaaatc
    13861 ttcaagacat cattttcaac tttaacagta agaactgggt aaccaggctg ttccaaccaa
    13921 gaatccatga aggctgcgac atcacgtcct gacgcttgac caagggcatc ccaaaggtca
    13981 ctaccaatgg tgttgctgta ttggtgtttt tcaaagtagg cgtgcaaacc tttagcaaaa
    14041 tcagcatctc ctagccaacg gcgaagcatg tgcatgagac ggcttccttt ggcatagacg
    14101 atagcgccgt caaagagtgt attgatttca tctggatgtt taacttcgac gtggacagac
    14161 tgaacgccat cagtagcgtc acgttcaaga gcaagaggta ctccacctgt ttggaaatct
    14221 tcaaagatat tccagcttgg ttcgatggta tccacacaga cgtattccat catattagca
    14281 aaactttcat tgagccaaag gtcatcccac catttcatag tcacgaggtt cccaaaccat
    14341 tggtgagcca attcatgggc cacaacaagg gcaacttgtt gacggctagc aaatgtagag
    14401 ttctcatcga caaccaagta aacttcacgg taggtcacaa gaccccagtt ttccatagca
    14461 ccagctgaga agtcaggaag ggcgatgtgg agagattgag gaattgggta cttaactcca
    14521 tagtaatctt cgtaaaactc gatagagcga acagcgatat ccagtgagaa atcaagattt
    14581 gaaagtggat gtgctttggt tgagtagaca cctaccaggg taccattttt agttttagcg
    14641 gtcacccctt gcaaatcacc agcaacaaag gccaacaagt aagaagacat gcgaggtgtt
    14701 gtctcaaact tccagatacc tgtttcctta cggttttcaa catcgatttc tggcatgttt
    14761 gacaaggcca attcaccttc tgcttggtca aagcgaagag agaggtcaaa agttgctttg
    14821 gcttcaggct catccacaca tgggaaagct tcgcgcgcaa aatggctctc gaactgagta
    14881 gacaagacct ccttcttgac tccatcaact gtataataag aagggtaaat ccctgtcatg
    14941 ttgtctgtaa ttttaccaga aaaggcaaga accaattcaa cttgaccagc ctcagccaat
    15001 tcgatatgaa gggcttcatt gtcatggtca actgtaaatg gacgagcttg acctgcaact
    15061 tctacagagg tgatttccaa gtctttttgg tggagggaga tgcggtcact ctgtgcttga
    15121 ccagtgatgg tcactttccc agaaaaagtc ttggtctcac gactcaaatc taaaaataaa
    15181 tcataatgtt caggaacaaa ttgcttaata aaatgttcaa ctgcttgcat agttttctcc
    15241 tattctaagt ttaagagctg ctgctcttct tcaaacaaac ttattatatc atattttgct
    15301 tgagaaaaaa ggattggaca gcgatttcca aaactttctt ccttctaata gtctacagtg
    15361 ggtagacctt gcgaaattct tctaaaacaa ccttgctttc aggtgtaaaa gtcagcccta
    15421 cttcacccaa acagtctgtg aaaaagtcct catgagcctg gcgccaatag gcaagtgatt
    15481 tgtctccctc accttcctta aaggcatgtt gcgcagagac ttgattgaag agttcaacag
    15541 aaacctttgt aatttcgaca atgcagacag actgattttg actatctaaa atgacatcga
    15601 aggtccctac ttggggaagt ggttcgtctt ctagtacata gaggtcatag gctgatgctg
    15661 ttgctgtctt ttctccttta aacaccaaat ccgctaaaag gtctggttca actccaaaag
    15721 cccaagcatc gatttcatct ccgatcaaag gattgatttg cttgtattta ttccacattt
    15781 cttgcggtat catgggtact cctttgtaat tttttacttt cttcttttat gtgtttaaga
    15841 tgatctggat ggtcaatctc taaatcaaaa atctctggaa tagaactgta gtggataatg
    15901 cacttgatac ccaactgatt cattttttgt atgaaagaag tattcagata gcctgctaca
    15961 gcaaaattaa tcttgttctt tcttgcttta tcctgcatat ctcttagcat atctaacatt
    16021 attggacttt ccatatcatg ccattgactg tttctcatag tcgcaaaaac aaaggaagtc
    16081 aaatcattca ttccaactac aatctttgaa atgcccgttt ccagtatact agataagtca
    16141 aaatacgctg acggtaattc aatcatcgtt ccgactttcc cagtaaagcc atactgacgc
    16201 aatactgtaa tagcttgttt taattggtcg gcatcattga cagaaggaaa gataacagat
    16261 agattggggt tggtttgata aacttctgta acgacatgtg cttcagcctg aaattcatcc
    16321 aaacacgcca gtaaacgtct ggttcctcta tagccaaaca agggatgccc ttcgtcaaaa
    16381 aactctctag tccccactaa acaattggct tctgtattcg ttaattcaga aaaacgatac
    16441 caaacttcct tacctaagta aaaggagcaa atagtatcaa gataatcttt tacaaattgc
    16501 tgacaacttg ctaatatgat gttttgattg agctctctca ataagtattc cccacgaatc
    16561 atgccgacgt ggtgaaatag ttgaggataa attttttcaa gaattttttc gccactaagg
    16621 gcaagttgat ttctcatagc cattcacctc gtcttaaaag atacaatatc atatataatt
    16681 atatcaaaat ttcctctagt tcaaaagctt cataaggttc agataactca tatcctaaaa
    16741 attctgctac ttttttggaa gcctcattat gagcatccca aagtggaaac atatctctat
    16801 ttaaactctc tagaatcatt gcagcaccaa gctttttagc aagtccattt ccttgttcgt
    16861 ttggtctagt tgcaacttcc acttcaactg ctttacggta aactaaccct gaggaaatcc
    16921 cagcaatcag ttcattattt ttaagaatca caaagccaaa tccacctttt aaaacaaaat
    16981 cctgatagga ctcaaaatcc ccctgtaaat cctgtgacca ttcttccgta gaaaagcagt
    17041 tataaatatg attatcaata ggcacaatat tgtaaccttc ctctaaatga gtaactagat
    17101 tatttaaaaa ttcaacttga aaatttgcct tatctttaaa agaatagcga gtaaaagaat
    17161 ttagccccac ttttcgatgc agaaaatctt gccaattagt attttctgaa ataataatct
    17221 tataatctaa accatatttt ctaacaaaat cttcccaaaa tctggaatcc aactcccctg
    17281 ctaggtatag aaaattccca acatcataaa agcttgttgt cctatcattg tttcggtaga
    17341 cagttccaat ttgagattta agaccataga gaaccatatt tttcttccat gttttaaata
    17401 gttccattat ccctctactt caccttccaa ttcatgtaag aagtcttgtc cagttctgga
    17461 aatcctaata attcagactt aaccttcaag actaatggcg atgcattttc ttctgtaatt
    17521 tcttgaatat ccatccaaat atatccaagt gaatcattcg caccatcaga cacagcttcc
    17581 gaagtcgtaa cttgaggtgc actctcattc atttcaacat catacaaggc catgacatgg
    17641 tgaaccataa aatttgttaa ctcttctctg acgaaaacat cgtagattcg aggattagag
    17701 tagcttctaa cagtaaatcc cgtctcttcc ataacttctc tagtcagcgt ttccgtcagt
    17761 ccttcaccaa gttgctgact gcctccaggt agatcatacc gatgttgata agggcctctc
    17821 gttttttcaa tgcaaagtaa ctttccattt tcaaagcaaa cagcgtagac tccaaagtgt
    17881 tttttgattt ccatccaact cctcctactt caaagaccag ccaccatcta ttgtcaagat
    17941 ttgtccttgc atggcgctcg cttttccact tgctaaaaaa agactaagct ctgctatttc
    18001 ctctggctca atccagcgct tgattggggt ttcactagcc acccagtcag ccaaaccacc
    18061 tggttcaaaa tccgcagcgg tcatagctgt cttgactgct cctggagcga taccaaagac
    18121 ctgaatccca gcttcagcat agtctagagc caactgcttg gtgaagccag ccaaggcatg
    18181 cttggatgaa gtataggcgt gaccacctcc acctgctagg ctagaagcaa tggaacacat
    18241 attgatgatg attccctttt tattttccag catttgtgtc aaataatacc gagtcaactc
    18301 tactggaata atgtagttga tttcaaaaat ctcttgaatg tcctgcgccg tttgttccaa
    18361 cagtggtttg taatcatcca aaactccagc agtattacac aaaacatcca cctgagggca
    18421 ccagtcaaaa ataggttcca agtccaaggt caaatctctc tgtaaaaagt gaaaatcacc
    18481 ctctaagagt ggcttttcac cttggtcaac tccataaact tgatagccct tctctaaaaa
    18541 gaggcgagct tgagccaatc cgatccctga actcactcct gtaatgagta cacgtttagt
    18601 catgcacttc tacccaatcc gttgccaaaa catcacaaac tgtcgggctc cacatggaaa
    18661 aaccttctcc ttcgccagaa acgttgatta ggaaataagg tgtcatttca agtgcaagcc
    18721 cattttgctc gatggtatca aagagttgga catagttttc cgcacctccc caaccagttc
    18781 gtacatattt tctcttagcc tttaacccag gcaggatctc ttcaaatgtc atgtttttct
    18841 cctttaattc tacattcttc atttaattat agcaaaaaac cgctttatac ggctttttga
    18901 atgtgagtta ttcaaacctg ctactactta cggcaaatta ttccctgcag caagataaat
    18961 ttcataccat tcttttcttg ttaagctaaa gtttgccgct tggctaactt ctctcaagtg
    19021 cttaggattt gtggtaccta caactgcctg catttttgct ggataacgca atatccaaga
    19081 aatggcaata gttgaagagg ttactccata tttaatagct aaacgatcaa gtacttgatt
    19141 taaagcttga aatttctcat ttccaacaaa attcccttta aaatacccga attgtaagac
    19201 agaccatgct tgaatgacca catcgtgtaa tttgcaatat tcaaaaatgc tgccatctcg
    19261 catagctgct tgactatctt ccatattaac atgaaaagct gattcaaatc ctggagtaaa
    19321 agccacactc aattgtagct gattaacagc taacggctgc ttgacatctt ttttaagcaa
    19381 ctccatcatc ataggatttt gattagaaac tccaaaatct cgaactttac cttgtttata
    19441 aaggagatca aaggcttctg ctacttggtc agattccatc aaagcatctg gtcgatgaag
    19501 gagcaagcta tctagatgat caatcttcaa tctttgcaaa ataccgtcta ctgattttat
    19561 aatatagtcc ttagaaaaat caaaataggt aaattcttca atgcgaatgc cacatttgga
    19621 ctgaatccac atcttttctc ttaaatctgg acgatttttt aggacaagac ctaacagttc
    19681 ttcacaacga ccacgaccat aaatatcagc caagtcgaag gcattgattc caacagaaag
    19741 tgctgtttct acaaactctt caacttcttt tacagactta tcttttattc tcatcattcc
    19801 gagaacaatt tctgataatt ctttgtcatc ttgaccaaga gttatgtatc tcatcaaatt
    19861 tttctccttt aatttctaac attcttccct tcattataac aaaaaaccgc tttgcaacgg
    19921 ctttttgact atacttcact ccattttatc ttcttaaacc catggaacaa gagaaaaatg
    19981 ccaataaaga ggacagctaa aggaataact tttgtaagga aaacatttga aattcccatc
    20041 cactcataat aacggagcag agaacccacc acaagatggg caataatcat actgacaaat
    20101 ggacgaaaga ccgcttcttt ccaattccaa ataccgataa ctagcgaaat cgtaaagaca
    20161 gacaaactat cccagggatc cggaatataa aaggctcctt cttgtatgaa gcttgccatt
    20221 cctacatatc ctaaaacaac tagaagaact atagtcccaa caacaatgta agtgccaaat
    20281 ttcattttag gagaatcttg gactaaactt cttcgtaaaa ttgtggccac aagtccaaat
    20341 ccaatcagaa aaataagaag ttgccctaaa aatgtgagca aattgactgt taagagagga
    20401 cctttagaaa aatcacttag tagttgataa taacgtaata ccgccaggac aagaattggc
    20461 gtcaaaaggg actctttgat agaactgcga ggtgctccct tgagaatctc tttcattatt
    20521 tttttaggat tcttacctag ataatcctct gcactcatgc catctcgttc tgcttctgag
    20581 aaatctagca tcatcaaata gatctgctct ctgagatagt cttcatcata gagaaatcca
    20641 gcaagattaa aactttccca caactcctca aaatactttt gattctcctc agaaaactca
    20701 tgtagcaaag cgcttgtttc ttcgtaatac ttcattttct tcatggttta acccccattc
    20761 ttaatccctt ctactttttg actcaaatcg tcccattgtt gccaaaagac tgagacacgc
    20821 tcttctcctt ctttcattaa tgaaaaatac ttccgatctg gaccatctgg cgacgggcgc
    20881 atgtcgcctc ttatccattg atttttttct aacttttgca acaaaggata aatagttcct
    20941 ggaacgatag tatcaaatcc agcctctcgc aaagtctgaa ccaactcata accataccgc
    21001 tctttttgac caatcatatc caagacacaa ccttcaagaa caccttttaa tagctgagtt
    21061 tctttcatca cttctccctt ctaatctatt ttgtaatacc tactagtaac ttcacctata
    21121 gtatattact tctacactag tttgtaaagc ataatagtta atactcttcg aaaatctctt
    21181 caaaccacgt cagcgtcgcc ctaccgtatg tatggttact gacttcgtca gtttcatcta
    21241 caacctcaaa aacatgtttt gagctgactt cgtcagtttc atctacaacc tcaaaaacat
    21301 gttttgagct gacttcgtca gttctatcta caacctcaaa gcagtgcttt gagcaacccg
    21361 cggctagctt cctagtttgc tctttgattt tcattgagta taaataaaaa aacagaacta
    21421 gtctgaacta gtcctgtcta cttttaccca atcacacttc catttggtac agctggatca
    21481 actgtgagaa gggttaattt gccatcatgt tcagctgaga gaatcatacc ctggctgaca
    21541 tattttttca tcattttacg tggtttgagg ttagcaacga tttgaacttt cttgccgacc
    21601 aattcttgtt catttggata gtattttgca attcctgaaa gaatctgacg atcttctcca
    21661 tcaccagcat ccaagcggaa ttgaagcaac ttatctgaac cttctacttt agacacttct
    21721 ttgacttctg cgacacggat ttcaaccttg tcaaagtctt caaacttgat ttcatccttg
    21781 tttagtttga gctcaacttc gtccggattc cattcttttt cgactgctgg tttattgcct
    21841 tccatttgtt ccttgatata ggcgatttct tcttccatat ttagacgtgg aaagataggt
    21901 gttcctttgg caactacagt cacatctgct gggaagtcag ccaaactcaa gttttcaaga
    21961 ctagaaactt cttccaaacc aagttgagtc aaaactgcac gactagtttc catcataaat
    22021 ggttcaatca agtgagcaac tacacgaatg ctggctgcca agtggctcat gacacttgcc
    22081 aattggtcac gaagagcttc atccttggcc aagacccatg gtgcggtctc atcgatgtat
    22141 ttattggtac gagagatcag agtccagact gcttcaagcg cacgtggata gtcaactgct
    22201 tccatgtgtg tatggaagtc tgcgattgat tgttctgcaa cctcagcaag aacatgatca
    22261 aattcagtca caccttctac ataggcaggg atttgtccat caaagtactt attaatcatg
    22321 gaaaccgtac ggttaaggag gttcccaagg tcattagcca attcatagtt gatacggccg
    22381 acatagtctt caggagtaaa ggttccgtct gaaccaactg gaaggttacg catgaggtag
    22441 taacgaagtg gatctagtcc ataacgctct accaacattt cagggtaaac gacattccct
    22501 tttgacttag acatttttcc gtctttcatg acaaaccaac catgggcaat caaacgatca
    22561 ggtaatttaa catccaacat cataagaagg attggccagt agatagagtg gaagcgaagg
    22621 atatcttttc ctaccatatg gaagactgtt ccattccaga acttgtcaaa gttaccatgt
    22681 tcgtcttgag cgtagccaag agctgtcgca tagttaagaa gggcatcaat ccaaacgtag
    22741 acaacgtgtt ttggatttga tgggacaggc actccccatg taaaggttgt acgagatacc
    22801 gccaaatctt ccaagcctgg ctcgatgaag ttgcgtagca tttcattaag gcgaccatct
    22861 ggcgtgataa attcaggatg agctttgaaa aattcgacca aacggtcttg gtatttgcta
    22921 aggcgaagga agtatgattc ttcagaaacc cattcaacct catgacctga tggagcaata
    22981 ccaccagtca catttccagc ttcatcacgg aaaacttctg ccagctggct ttctgtaaag
    23041 aattcttcgt ctgatactga ataccaacca gagtattcac ccaagtagat atcatcttga
    23101 gcaagtaagc gttcaaagac ttgtgcgaca actttttcat ggtagtcatc agttgtacgg
    23161 ataaatttat cgtatgagat atctagtaat tgccagagtt ctttaactcc aaccgccatt
    23221 ccatcaacat aggcttgagg tgtaatacca gcttcttccg ctttctgctg gattttctga
    23281 ccatgttcat caagacctgt cagataaaat acatcgtagc ccatcaggcg tttgtaacgt
    23341 gctaggacat cacatgcgat agttgtgtag gcagaaccga tatgaagttt cccagatgga
    23401 tagtaaatcg gcgttgtaat ataaaaattt ttttcagaca taatttttcc tttccaggca
    23461 aatgaaacct gtttttctaa cacttcatta tatcacattt ttaatgaatt tcaataggga
    23521 aatccataca aaaacaagat agacgagtgt ccatcttgtt gatctcattc ataacgaagg
    23581 gcttcaattg gatcaagttt cgatgccttg ttggctggca agactccaaa aatcatacca
    23641 acactagccg aaactgcaag actaaatagg gcgactggga ttgatactcc aacttctata
    23701 ccttctatta aaccttgcag taacaaacct gctaaggcag ttaaaccact tgcaattgtc
    23761 aagccaatta agccacctaa caaggtcaaa atcatggatt caatcaaaaa ctgaattaaa
    23821 atattggcac gtgttgcacc caaagcctta cgaagaccaa tctcacgagt gcgctctgtc
    23881 accgaaacca gcatgatgtt catgacacca gttcctccaa caaagagaga aatccctgcg
    23941 atggaactaa taatcgtcgt cataaaacta aacgattgtt gaatttctgc aaatacaagg
    24001 gactcatctg ccacctggta ttctccctgt tgtaagcctg caagctctgt catttttcgt
    24061 gccagttctg gacccagagt tggggttaaa ctggtatcat tcactcgaaa gacaatacta
    24121 gctatttcat ctacattaaa attcgcagca agggagatat tggtagtaat aggcaagcca
    24181 ccaaacccat atatttttga tcttttagcc tccggactag tataaacccc aatgacccgg
    24241 taactaaatc cattgacttc tacaaccttg ttaatagcct cttgaggaga ttcaaataaa
    24301 ctaatggaca attcctcatc tagcaaaata acacttgcaa actctttgaa atcttgctct
    24361 ctcagactac gacctgcaat aatttcattc ttaacagcgt ccatgtaagt tctgtttcca
    24421 cctgtcaaat tagcattctc aaccttttta tcttgatagg tcaagatggc attcgttgaa
    24481 ttggttacat agtaactatc cactcccttc agtttagctg cctcttggac ccaggattct
    24541 tgcggttttg gcggttcaac aggaacttcc tcttcctttc cagaaaccgt aaaagctgat
    24601 tgtttctgag taaaagaccc atctttactt tttttaggag agaaaaagac gctaatattt
    24661 ttctgagatt tagtcatatc tttattgact tgacgagata gggaatcacc caaagccata
    24721 atcacaacaa ctgatgaaac accgataata atcccaatca tagtaagcaa agaacgcatc
    24781 ttgtgagcca tgatagatga aaaggcaaat ttcagattct gcatcttagt tttcctcctt
    24841 tcctaactga gcactgtcag acgaaatgac cccatcccga atgacaatct gacgtttggc
    24901 ataggcagca atctcaggct catgcgttac catgataatg gtttttcctt ctttattcaa
    24961 atcaaccaat aattgcataa tttggttacc tgttttggta tccaaggctc ctgtcggttc
    25021 atccgctagg ataatagaag gattgtttac caaggcacgc gcaatggcta cacgttgctt
    25081 ttgaccacca gataattctg aaggtaaatg gtgactacgt tctgtcaatt caaccttgtc
    25141 taaatattcc tcagccaact tgcgacgttt tgaagacgaa actcctgcgt aaatcaaggg
    25201 caattctaca ttttgcagag cattgagctt cgatagaaga aagaactgct gaaagacaaa
    25261 accgatttgt tggttacgga ccttagctag ttgtttttca ccaagcccag ccacttcttg
    25321 accttcaaga taatattctc cactggttgg tgtatccaac atgccaatcg tattcatcag
    25381 agtggactta ccagacccag atggtcccat gatggctaca aattcaccct cattcacttc
    25441 tagattgata tttttgagaa cctgcagttc ttggtcacca ttacggtaac ttctgaagat
    25501 attttttaga ctaattagtt gcttcatcag ccttcacctc ttttccttct tccaaggaag
    25561 atgttggatt actgatgacc ttagcaccgt tcgttaaacc agaagtgatt tcttgatttt
    25621 ctgcgtcagc atttcccaat gaaacctcaa cttttttagc cttttgttgt tcatccacaa
    25681 tccagacata atttttacta tcatccatta ctagactgct aacaggaaca agaatagcct
    25741 tagttttgct tttaacctca atgttgacag aaaaaccttg tttcaaatca ccaacctcgc
    25801 ctgtcacatc aatagtataa gggtatttag aacctgtatt attcccggct gctggactag
    25861 ctgcttcacc attgttttta ggatagtcag aaatatagct taatttccca gtccattttt
    25921 tatcaggata cactttagaa gtaaagctta cttcttgacc tacagaaagg ttggctagat
    25981 tgtactcaga caattctccc ttgacttgta aattttcatt gctgacaata tgaaccataa
    26041 cttgactcgc ccctgttgga gatttagaaa cattgctatt gacttcgact acagttccct
    26101 ctagggtact gagaacagtt gttgcatcca attgactttg agccttgctt aattgcgctg
    26161 cagcatctgc acgcgcatca cgggcatcac ccaattgagc atcaatagaa gcaacagaat
    26221 ttccagccac tggagttggg ctttgcaccg ttgcatcttc tcctcctact ggcgctggta
    26281 actgtggagc ctgagctgaa gcggcttcat ttcgtgcttg attgagttca ttgatatgac
    26341 gatctgcctt agctactgct cgactagctg aatcataggc cgcctgcgct tctgaactac
    26401 tgtacttgac taaagcctgc ccttcgctga ccttatcgcc cacagaaaca aggatttcat
    26461 ctaaatcacc cttactagca tcaaaataaa catattgttc attttttgct gttactgtcc
    26521 ctgacaataa aacagaggat gccacgcttc cttccttggc aacaacaaga tgagtaggct
    26581 catcttttag agcagtctga gaaggttgtc taaaaagtaa aatccccccg gcacccaata
    26641 caactacact cgcagcaccg attgctgcat acagttgcca ctttttagct ttaccattct
    26701 ttttcttcat aatgaaactc cttttctttt ttacaatact ttgctattat accaaatttc
    26761 cctccagcaa acaatgcagt tcagatggaa ataatggcag ttcaggatta aacaatcgtt
    26821 cggaattttg cttttctatt gtactagata tataatacac tatcttgact tatttggcaa
    26881 gcttttttgc aattttttgt gcgtagcaga tttttgcaat caaatcaaaa aaagaataaa
    26941 atataactat caaaaaagac gctctactat ttaaaagatt acaaaggagt tttcaatgag
    27001 agaatatgat atcattgcta tcggtggagg tagcggagga atcgctacca tgaaccgtgc
    27061 tggtgaacat ggagccaaag cagccgttat tgaggaaaag aaattaggtg gaacctgtgt
    27121 caacgtcggt tgtgttccta aaaaaatcat gtggtacggg gcgcaaatcg ctgagacttt
    27181 ccatcaattt ggagaagact acggctttaa gactactgat cttaactttg actttgcaac
    27241 cctacgtcgc aatcgtgaag cctacattga tcgcgctcgt tcttcttatg atggtagttt
    27301 taaacgcaac ggtgtagact tgattgaagg tcatgctgaa tttgtagatt ctcatactgt
    27361 aagcgtaaat ggtgaactga ttcgtgctaa acatatcgtg attgctacag gtgcccatcc
    27421 aagtattcct aatattcctg gtgctgagct aggtggctct tctgatgatg tatttgcctg
    27481 ggaagaactt ccagagtcag ttgccattct aggcgctggt tatatcgccg ttgaattagc
    27541 tggcgtactc cacacttttg gtgtcaagac agatctcttt gttcgccgcg atcgtccttt
    27601 acgtggtttt gattcctaca tcgttgaagg tttggtcaag gaaatggaaa gaacaaactt
    27661 accacttcac actcacaaag tccctgtcaa gttagaaaaa actactgacg gcattaccat
    27721 tcatttcgaa gatggtacta gtcacacagc tagccaagtt atctgggcta caggccgccg
    27781 tccaaacgtt aagggcttgc aacttgaaaa agctggagtg actctgaacg aacgtggctt
    27841 tatccaagtg gatgaatacc aaaatactgt tgttgaggga ctctatgctc taggtgatgt
    27901 aacgggcgag aaagaactga ctccagttgc aatcaaggcc ggacgtaccc tatctgaacg
    27961 tctctttaac ggaaaaacta ctgcaaaaat ggattactca actattccaa ctgttgtctt
    28021 ttcacaccct gctatcggaa ctgttggttt gacagaagag caagctatta aagaatacgg
    28081 tcaagaccaa atcaaggttt acaaatcaag ctttgcatct atgtactctt cttgcactcg
    28141 caaccgtcaa gaatcccgtt tcaaactcat aacagctggt tcagaagaaa aagttgtcgg
    28201 acttcatgga attggctacg gcgttgatga aatgattcag ggatttgccg ttgctatcaa
    28261 aatgggagca accaaggctg actttgatgc aactgtggca attcacccaa ctgcatctga
    28321 agaatttgta accatgcgtt aatcgaaaca aaagaagctt atataaatat gtctgcttct
    28381 tttttaatga ttttcgaatc aacaaaaaaa ttatccaact tgactagtca cgaaaatcgg
    28441 ctgttacagt ttttgttacc ttgtttttaa aaaatggttg acatcgactc ctatatgagt
    28501 ataatagtta ctatacatta gaaaagaggt taactatttt gaaaaaagct cacgtttatg
    28561 ctatccctgc tattggggct gctctcattg ctgttttggc acaaatcagt cttccaattg
    28621 gacctgttcc cttcactctg caaaactttg caatcggctt gattgctact gtctttagac
    28681 cgagagaggc tgtactttct gctggactct atcttcttct aggtgctatc ggtcttcctg
    28741 tctttgcagg aggtggagct ggttttcagg ctttagttgg ccctactgca ggctatcttt
    28801 ggttttatct cgtttactct ggacttactt cctctctaac caacagcaag agtggtgttg
    28861 ttaagatttt tcttgcaaac ctcttgggtg atgcccttgt ctttgtcggc gggattctca
    28921 gcttgcattt cctagctgga atggcatttg aaaaagctct tgttgtgggg gttcttccct
    28981 ttatcattcc agaccttggc aaacttctgg ctattagttt tattagccgt cccctacttc
    29041 aacgccttaa aaatcaggct tactttacta actaaaaaag gataccgagt tatcatgact
    29101 caatatcctt tttttatttt gaaaacttat actcaatgaa aatcaaagag caaactagga
    29161 agctagccgc aggctgctca aaacactgtt ttgaggttgt ggatgaaact gacgaagtca
    29221 gtaaccatac atacggcaag gcaaagctga tgcggtttga agagattttc gaagagtatt
    29281 agttgccttt aaaggcatcc accatcgttt gaaattcttc atttgagaga gtaatccctt
    29341 tgcccatttt agtatggtct ggactccaag cacgaatatc aaactttgca gaggcaccat
    29401 taaagctcac acggttaatt tccttggtcc aacctttttc gttttcagaa agagtcaaca
    29461 agtgctcttc gatttcaaat gtaaattctg ccattttctt ctcctttttt agtttcatta
    29521 gtttattcgt aaaatcttgt agattttagg aaaattttat ataatattga tataaaagaa
    29581 gggaggccaa tatgagacat aaattccagc aagttctaaa taaaatacat gattttttaa
    29641 atggatatga ccaacctgac cagactgaaa ccaactccct tacagccact attgaagagg
    29701 ctatccagaa acaaaccgct gttcacctta tcttatctga gacaagcttt acaggtgaca
    29761 tcatcaaata tgatcagcaa ggccagcaaa ttatcgtgaa aaatttttcc aaaaatgtga
    29821 gtcggattat ccgtataagc gatattcaac gcctgcgatt tgtcccctca actgtccaaa
    29881 cagcccaaaa aaatagattt aagaaagagt gagatgtagt tgcttcatcc cactcttttt
    29941 tcttagcgaa tttgttcaaa atgtaaatga actgcaatat gatctccata accacttctt
    30001 tccaagtcac gttgtaaacg ataggaaatg tagtgttctg caatggtaat gtaacctgcg
    30061 cccaataaac gatgttcaac catagattga atcatactga tagtcgcacg ttccaccttg
    30121 gcttcttgta aatccaaaac taccttctta gtgacttgag caagattttg acgcaaatca
    30181 tctgtcaaaa cataaacagt ttgggctgcc ttcaagatgg cttggtaaat cttatctgga
    30241 ttaaattcag caatttcgcc attacgtttg attacttgca taggtttctc ctttattctt
    30301 tgttttcttt gatttctgcc agcatttttt cttcttctac tgtcagttga taatgttcaa
    30361 gtaaatccgg tctgcgctcg taggttttct ttaaactctc gtacaatcgc cactgacgaa
    30421 tcttttcatg gtggccactc atcaatacat ctggcacgac catgcctcga taatcatagg
    30481 gacgtgtgta ctgaggatat tctaaaagac ctgaagaaaa actatcatct tggtggctag
    30541 actccttgcc aatcacttct ggaatcaggc gaactgtagc atcaatcatg gtcatagctg
    30601 ccaattctcc accagtgagg acatagtcgc ctagggaaat ctcatctgtt accaaggtct
    30661 taatgcgctc atcataaccc tcatagtgcc cacagataaa gattagctct tcctcttgag
    30721 ccaaatcttc agcataagcc tgatcaaact gctttccagc aggatcgagg agaataacgc
    30781 gcggattttt cttttcaata gcatcaaagg aatcgaaaat aggttgtgct ctgagcaaca
    30841 tgccctgacc gcctccgtag ggctcatcat ctacatgacg ggccttttca gcattttctc
    30901 gaaaattatg atactggata tccaagagcc ctttttctcg agcctttcca acgattgagt
    30961 gctccagtgg agaaaacatc tctggaaaga gggttaaaat atcaatcttc atcgtctaac
    31021 ccttctaaga tttccacatc gacccgttta tttggaatat caacattgag aaccactggt
    31081 gggatataag gtaaaagcaa atcacgtttg ccttttcgtt tgaccaccca gacatcatta
    31141 gcacctggtt gcaggatttc cttgatggtt ccaaccaagc tatcaccctc atagacttcc
    31201 aaaccgataa tctcgtgata gtaaaattca ccatcgtcta ggtcattcaa atcttcctca
    31261 gcgaccttga gactgtatcc cttgtacttt tcgatagtat tgatatggta catatctttg
    31321 aatttaataa tgtcaaagtt cttctgttta cggtggctag cgatggtcac tgtttggaca
    31381 aactgatctt tttcatcaaa caaagccagc tcagctcctt ttttaaaccg ttcttctgca
    31441 aaatccgtca cagacaagac tcgcatctca ccctgtaatc cctgcgtatt aacgattttc
    31501 ccaacattaa agtagttcat cttgtctcct gtaatctcct tttttccatc ttattctgac
    31561 aattctcgaa taatagccgc aattttttcc tattctgacc attgtaaata atggtgattc
    31621 cttcctaaaa tgagtttagt attggaagtc caatattctg attctctgta ctctttttct
    31681 ctataagtct gacaaaaaat aaatacaggg acatgagatt ctatcgacaa attctcaaaa
    31741 tcttcctcag taatctctcc agatatctga aattctggat cttgattttc caactctaag
    31801 cctttttctt gcattaattc ccagattttt ttattcgttt caggactaaa tgttgcttga
    31861 gttaagttct taaaataaag ttcaggacca cactcgtcaa tcagcctcat ctgctcttcc
    31921 atttctggat aagaattttc taaaaaatca gcaaacatga ctttttagtt gtcggttcaa
    31981 ttgctactaa agtctgacgc ttaattggtt tctcgagtaa tttgcaagct aaaattccac
    32041 tccaactatg tgcacaaagt atatattcag aaattcctaa ttcttcaagt acttcataaa
    32101 ccgcatctgc aagattatct agattttttc cagcttggtc atgaatcgga ctcctacctg
    32161 tgttcggaaa atcaattgtc aaataaccaa ttgtaggagg aggtttttca agtataagtg
    32221 aaaaattttc ataacttggt agcaaacctg ctccgtttag acaaactagc actttctttt
    32281 gcttttgata agcaacagag agactaccaa tttctgtaga tacttcaaac ctcttcataa
    32341 agaaatccac tcattctata taatgaatta ttaaaaatcc ttatctttta ttttatcacg
    32401 ttccaaggat tttctcaagt tggaggaagg ggacaacatc tctactttcc cttcaaaaat
    32461 ccttccaaat catgtttatg ttggtaatta acggctgcgg ttttgtcttt ctcaaagaca
    32521 gtcttggtaa ggtcaatatg attaatagct acgattgcga cggtgtagta aatgatatca
    32581 gccagttctc tggcaagttc ctcgttcgaa tcctatccct tcttttcgac cagagcgcct
    32641 attcaaaacc tcggctactt ctccgacttc ctccactaac ttcataaaga gaccttcatc
    32701 agtccgagac tgctgttaat gttcgagtaa gtagtcttgg aattgcctaa acgttcaatc
    32761 ttttatagta tattgaaact agaatagtac acctttactt ctaaaacatt gttagaaatc
    32821 gatttgactg tcctgatcga tttgtcctgt tattatttca ttttactata tcttctattc
    32881 cacacaaaaa agcgagacat ccgtcccgcc cttcttattt ttcgtcaata acgattctta
    32941 cttttttgta ttcagttggg acagagtaga caatcgttct tatcgcagaa atagtgcgac
    33001 ccttacgacc gattacacga cccacatcgc tttgatcaag attcaaatga tattccaaaa
    33061 attctggtgt atcttcaatc ttgatagtta aggcatctgg ttgtgaaatt aagggtttca
    33121 caatcgcaat aatgagattt tcaatcgtat ccatctgtca acctacttta aacttatttt
    33181 gagaatttag aatcgtggaa ttttttcaat acgccttctt ttgaaaggat gttacgtact
    33241 gtgtctgaag gttgagctcc attagccaac catgcaagaa cgcgatcttc tttcaaagtt
    33301 acttggtttt cagcaacaag tgggttgtaa gttccaactg tttcgatgaa acgtccgtca
    33361 cgtggtgaac gtgaatctgc tacgttgata cggtagaaag gttttttctt agaacccata
    33421 cgagtcaaac ggattttaac tgccattttt aaagtctcat ttctttaatt ttttatttcg
    33481 gtgaaatagc tgagctattt agcacatgtt ctattatagc agatttctgg catgtgtcaa
    33541 gaaaaaaact tgacagacta taaatttctt aaagtattta gaaatttgtt agaataggaa
    33601 ataaatgttt gaaagatgct atccgtgaat actattttag aaaaatttta taaagaacac
    33661 caagtcaaac ccttcatatc gcctgaacga gagttggata cttggcttct aagtcccaag
    33721 cccgttccca agcgaaatat ggacttatta gcagatgatt cactagcagg agatatcatt
    33781 ttactatgga gaatccagtt tggtactttt accactgaaa catgatttta aaaactccca
    33841 ttcgaatatg attcaaaatt cgcttagaat caatgttttc ccactttttc aaaaaataaa
    33901 ttttcatcta atttaaataa ctttcaatta agtgtgaatt ttgcttaaaa tcattaaaaa
    33961 acaccccatg gtgtgaacca tgaagtgttg taaatgctgt gttgtagcta gttcttagcg
    34021 tttagagaac tggctagctt tacgagcttt cttaagatct ggtttccaaa atactttgag
    34081 tacacttatg gtattgatgc ccctaaacat ctaaaaactc tggtagaaaa agggtatgct
    34141 cttgtcgaaa cttcttttga ctcacttgac catctaaata ctaccatgaa aaagaatatc
    34201 ctgaagaaga agggagttac tggactttct aaaatgaaat cttgcgacct ggatcaagcc
    34261 cttcatgagc atttttcaga agaagaatta gctggtcact ttcatgtcct tctatggact
    34321 tttttacaat ggcattgcta tcacacccaa tacctatcta agcgcctggt tcgtaaactt
    34381 tattgcagct cttcctctaa atttcctaat tgttgaacca attgcccgtt ttatactaag
    34441 ttcttttcag aaaccattta ctggggaaga agttgaagaa gttgaagatt ttcaagatga
    34501 tgatgaaatc ccaactatta tctaagccag ttctgtaaac tactaatatt tgaaatccac
    34561 ttccttttag ggtacaatgg ttataaatga atttttgaga ggatcagaat gaaaaaacta
    34621 gcaacccttc ttttactgtc tactgtagcc ctagctgggt gtagcagcgt ccaacgcagt
    34681 ctgcgtggtg atgattatgt tgattccagt cttgctgctg aagaaagttc caaagtagct
    34741 gcccaatctg ccaaggagtt aaacgatgct ttaacaaacg aaaacgccaa tttcccacaa
    34801 ctatctaagg aagttgctga agatgaagcc gaagtgattc tccacacaag ccaaggtgat
    34861 attcgcatta aactcttccc taaactcgct cctctagcgg ttgaaaattt cctcactcat
    34921 gccaaagaag gctactataa cggtattacc ttccaccgtg tcatcgatgg ctttatggtc
    34981 caaactggag atccaaaagg ggacggtaca ggtggtcagt ccatctggca tgacaaggat
    35041 aagactaaag acaaaggaac tggtttcaag aacgagatta ctccttattt gtataacatc
    35101 cgcggtgctc ttgctatggc taatactggt caaccaaaca ccaatggcag ccagttcttc
    35161 atcaaccaaa actctacaga tacctcttct aaactcccta caagcaagta tccacagaaa
    35221 attatcgaag cctacaaaga aggtggaaac cctagtctag atggcaaaca cccagtcttt
    35281 ggtcaagtga ttggcggtat ggatgttgtg gataagattg ctaaagccga aaaagatgaa
    35341 aaagacaagc caactactgc tatcacaatc gacagcatcg aagtggtgaa agactacgat
    35401 tttaaatctt aaaaaccaaa aaaaatacag tatccacatt cggtactgta tttcttttac
    35461 tctcattctt aagttaaatt attaaaatcc catatttggt ctatccagcc ttcataaaag
    35521 tctagctcgt ggcagaccat aaggatagat cccctatatt ctttgagagc gcgtttgagc
    35581 tcatcctttg catccacatc caaatggttg gtcggctcgt ccagcactaa aacgttgttt
    35641 tcacgattca tcaagagaca gaaacgaacc ttggcttgct ctccccctga taatacttga
    35701 atctggcttt caatatgttt ggttgtcaaa ccacaacggg caagggctgc acggacttct
    35761 gcttgattaa gggcaggaaa ggcattccag acagcttcaa gaggagtttg gcgattaccg
    35821 ccttctactt cctgttcaaa ataaccaagt tctaaataat ctccacgctc cacttcccca
    35881 gcgattggcg agataatgcc caagagactc ttcaagagag ttgtttttcc aataccatta
    35941 gcaccaataa tcgcaacctt ttgattgcgt tcgaaggtaa gatttaaagg cttagtaaga
    36001 ggacggtcgt aaccaatttg caagttcttg gcttggaaga taaagcgccc tggtgtacga
    36061 gctggtttga aatcaaagga tggttttggt ttctcacttt ggagttcgat aatatccatc
    36121 ttatccaatt tcttttgacg agacatagcc atattacgag ttgcaacacg ggctttatta
    36181 cgagccacaa agtccttgag gtctgcaatc tctttctgct ggcgttcgta ggctgcctct
    36241 agctgagatt tcttcatagc ataaacttct tggaactggt agtagtcacc agagtaacgc
    36301 gtcagctgtt gattttccac atgatagaca atattaataa cgtcattgag gaatggaata
    36361 tcgtgcgaaa tgagaacaaa ggcattctca tagttttgga gatagcgctt gagccaatca
    36421 atatgctcag catccaagta gttggtcggc tcgtccaaca gcaagatatc aggcttttca
    36481 aggagaagtt ttgccaaaag caccttggtt ctttgcccac ctgacaaaga agttacatcc
    36541 gtatccatgc caaagtccat aacaccaaga gcacgcgcta cttcgtcaat cttagcatcc
    36601 aaggtataga aatcacgact ctccagacgg tcttgaagtt ctcctacttc ttccatgaga
    36661 gcatcaacat ccgcgccgtc ttcagccatt ttcatataga ggtcattgat acgagcttca
    36721 gctttgaaaa gctcatcaaa agccgtacgg agaacatcac gcaccgactg tctttcagca
    36781 aggacagagt gctgatccaa gtaaccagcc gtcacatatt tggaccactc aacctttcct
    36841 tcatctggca gcattttacc agtcacgata ctcataaagg ttgattttcc ttcaccattg
    36901 gcaccgacca ggccgatatg ttctcccttg aggagacgga aggacacatc ttcaaaaatt
    36961 gcacggtcac caaaaccgtg actcagattt ttaacttcta aaatactcat tttaattcct
    37021 tatcttgttt ttatgtaatc gtttataaag gagccaagcc agatagccac ccaaagtgtt
    37081 ggtccacaaa tcatcaatct caaagacgcg attgaaatca aagaaaaagt ctaagattaa
    37141 ttgcgtacac tcgattccaa gactcacaag aaaactaaaa agaaggacct tttttgtttt
    37201 ccgcaaattt ggaaatagat aaaggagttg gaaaatcaga ggaaaaaaca agaagacatt
    37261 gaggatattt tgtaaaaaaa tccaacataa ttgtccaatg tcactcactt cgcccagttt
    37321 ccagagagaa ttgaaaggag tcaaaagaaa aaccaggcgt ccaagatgct gaatacctgg
    37381 agttcccact cccacggtag attgttcttg aggagtaaag caaaaacaga caatgcaaat
    37441 gctatagaaa atgactcccc agaccaaaat atgattataa gtcttcttca tcattaagga
    37501 tttaccgctg cgactgcctt ctggcggtca cgtttcattg tgttagagcg caattgtcca
    37561 caagctgcgt caatatctgt accatgctct tgacgaacca cacagttgac cccttttttc
    37621 ttaagcgtat catagaaagc caacacgcgc tctttgggac tacggctata ttggtcatgc
    37681 tcactaactg ggttataagg aatcaagttt acataagaca atttcttgat gttcttgagc
    37741 aattcagtca attccaaggc ttgttctaca ccgtcgttga cttcattaag catgatatat
    37801 tcaaaggtta caccacggtt tgttgtctca atgtagtatt caatagcagc aaagagtttt
    37861 tcaatcggaa aggcacggtt aatcttcatg atacttgaac gaagttcatt gttaggtgcg
    37921 tgaagagaca cggcaagatt gacctgaacc ccttcatcag caaagtcacg aattttatga
    37981 gccaaacctg aggttgaaac cgtgatgtga cgagcaccga tagccattcc tttatcatca
    38041 ttgatagtac gaaagaaatt caagacattg ttgtaattat caaagggctc accgattccc
    38101 atgacaacga tatggctgat gcgttcatcc tgaccacgct catcaaagta tttctgaacc
    38161 agcatgattt gcgctacgat ttcaccgtta ttgaggtcac gttgcttctt aatcaaacca
    38221 gaggcacaga aggtacaacc gatattacag ccgacctgag tggtcacaca gacagataaa
    38281 ccatagtgtt gacgcatgag tacagtctca attaacatac cgtcgggcaa ttcaaagaga
    38341 tatttgactg taccatcagc agactcttgc acaatacgtt gtttcaaggg attgaccaca
    38401 aactggtcat tgagcttagc aatcaaatcc ttggaaaggt tggtcatttc ttcaaatgac
    38461 tgcacacgtt tacggtagag ccattcccag atttgatctg cacggaattt cttttctccc
    38521 tgctccaata cccattcctg catggtttga tgtaccaaac tatgaattga gggtttcatt
    38581 tcttctcctt attctctact cacttctgac gaatgacaaa atgacgttgt cccttgtcct
    38641 ctttctgaga atgctggtct ttctgacgac gtctattttt cttatctgca ttcgactttc
    38701 gtttagtttg agtcggtttc tttccttttc tagaaggtgt ttcttcttcc gtcttacgca
    38761 ttttcttgtc aaatgatgct cgcttagggg cttcattttc taagacaaaa taggcacaac
    38821 cataactaca atactctaaa aggtagtctt gtaaacgact gattttttca agtttttctt
    38881 ctgttcggtc atccttgtaa aaacctcgta ggcgaagctg ttcgttgctc cagtccccca
    38941 cgatataatc aaacttggtt aagacttctg aaaaacgctg attaaaagtc gtcacatcaa
    39001 aggcatcctt gatattttca accaaggaaa aagctatccc ttccgtttcg accttgtccc
    39061 cgtgtaaatg gaactccgga ccaggaaact tgttatagtt gtataattca ggtgcaattt
    39121 cttttcgcat agatatcctt tttccacgat tacttaatac tttattctac cataatttct
    39181 agcagttagc acgtttctca taaaaatgaa aaaagtctga cgattttgtc agaccagaat
    39241 cttataacct aaaaagagaa gaacaattct tccctccaac tatcattatt tagcagctgc
    39301 gtacaattca tctactttat tccagttgat tactgaaaag aaagctttga tgtagtcagg
    39361 acgcacgttg cggtatttca cgtagtaagc atgttcccaa acgtccaagc ccaagattgg
    39421 ttttttacct tctgagattg gtgtgtcttg gtttgctgtt gaagtcactt caagtttccc
    39481 ttctttgttg acaaccaacc atgcccaacc tgaaccaaaa cgagttgttg ctgctgcagt
    39541 gaaggctgct tggaattctt caaatgaacc aaatgttgca tcgattgctg ctgccagttc
    39601 tgctgaagga gctgttttct cgggagtcat caattcccag aaaagagcgt ggttcaagtg
    39661 tccgccacca ttgttgataa gtgcttgacg gatatcagct gggatagatt ctacatcagc
    39721 aagcaaggct tcaaggtctt caccgatttc agggtgtttt tctaaagctg cattggcatt
    39781 gttgacataa gtttgatggt gtttgtcatg gtgtaaatgc attgtttccg catcgatgta
    39841 tggttccaaa gcgtcgtatg catatggaag ttctggtaag ataatagcca tctgtaatac
    39901 ctctttttct ttctatatga aaatgataac gcaaacattc acttttttca agttttttgc
    39961 ttatagataa ggaaatcact gacatacttt ctaataaaat aagcaaatca gcaagaaacc
    40021 cacgacaaga taatcctgac gcgggttgta gtttcaacaa atgtcaattg acctgactag
    40081 caatctgtaa gagtgccttt tcaaaaagga aacctttttc ataaagacct gtcttaatct
    40141 gatagtctgt ctcaatcaaa taggaaatag cttgcttcag aaagctcaaa gaaagtcctc
    40201 tcgaatctct taatgcaaac ttgatttgat aaggatttgg gttacgtccc agataactac
    40261 ctaaactact tgcaatctgc gattctgttt ggccagactc cgccaaaatc ttcacctgag
    40321 taaaagtccg aaattgtccc agcatgactg caatcagttt gatttcatct tccccttgca
    40381 aggtcaagtc tctcaccaaa tcgcgcgcct gatccatctt tttagtcaga ataaactgag
    40441 ttaaatcaaa aatattgtcc tgcaaggtct tgggaattgc gttaacaata tcctcttcct
    40501 cgataacaga atccgcctta taggactgta aaaagagaag atttttctgg atttcgctaa
    40561 attgaaaccc cgacttgatg aggagatttt caaaagaatg attggtaaac tgcagacctt
    40621 gtttctgact ccacttttgg aaatactggc gcaattcttg ttcttttact tctactgcat
    40681 cgaagacctt ggcatcacgc ttaagtaatt tgactaaccg tcttttgcta tccagctttc
    40741 cttctgcaaa gattatcaac ttggttgttg gagaaggatt gtcaaggtat tcctcaaatg
    40801 acttaagctc atcatctgtc aaaaagcgtt tcttagcagt cgtgatatcc ataaaataat
    40861 ctaatatcac tattttctca tccgcaaaga aaggaaggct gaccaactcc agttccacat
    40921 ccttgtaaac tacttctttc atatcaaagt aggcaaagtt gaggtcagca gaatcatacc
    40981 caatctgttt caacatttga ctcttcatca cttcaaactg accctgatct gtccctgtaa
    41041 ataggctcag gctcggtaaa tttgataaag tcaacttctg actttcttca atggctagca
    41101 tcgactctcc tttcttcaga tttttcgatt taatttagtc aatatagcgc aatttcccac
    41161 ggaaatcttc taagctctcg tagccttttt ccaccatgat tgctttcagt tcattggtaa
    41221 tgcggtcaaa agcactgacg ccttctttgt gaagggtcgt tcccacctgc accatacttg
    41281 ctccacagag gatgtgttca aaggcatctc gaccagtcag aacgccacct gttccgataa
    41341 tttggatttg aggatttaaa cgttgataaa aggcgtgaac attggctaga gcagtcggtt
    41401 tgatgtattc tccaccaatt ccaccaaaac cattcttagg ccgaataacg acagattcgt
    41461 cttctatata gaggccgttt ccgatagagt taacgcagtt gacaaacttg agcggatatt
    41521 tgttgaaaat agctgccgct tggtcaaagt gaacaatatc aaaataaggt ggcaatttaa
    41581 ttccaagagg tttggtgaag taagcaaaca cttctgccaa aatccggtct gttgtctcaa
    41641 aatcataggc aatctgaggt ttacctggaa catttggaca ggaaagattt agctcagtca
    41701 gaccacgaaa atcactctct tggacttttt tcaaaatagt atgggtttcc tctggagaca
    41761 tgccgaccag agataagaag aaagttcggt tcgactcttt ttcctgcaaa tctaaaagat
    41821 aatccaaata atagtctaag ccattatttg gcaagcccat agagttgatg gaaccaagtg
    41881 gaacatcttg gtagcgtggc tcaggattcc cctgacggaa gtccaaggtc gctgtcttag
    41941 taacaaaggt tcctgccgct gagtttttga cctcttctaa ctcctctatc gtcatacaag
    42001 ccacacctgc tgcattcatc aagcaattgt caaactcaaa accagcaatt tgtgttttcg
    42061 ttgataccat gattctaatc ctccagattt cttttattgc taatattata ccataatttt
    42121 aattctaggt ttactagatt ctgtggttct atctatttat tcggaaaaga aagtctagta
    42181 cagaagtcat gctatggaaa cagctagtgt attggaagtt tgaacaaggc ttgggtaaca
    42241 gacttttgaa tgaagtccaa gcaacaagga gaaatctcaa ccatgagtcc attccatgac
    42301 tctgttgctc tagaaaacct tgcaatagtc aagtgataac gaataaaact ttggattgaa
    42361 atatccccat gattatctat tacgaggaga aatctcaacc tttatcattt ttagagaaat
    42421 atagtaaaat gaaacaagaa caggagaaat cgatcaggac agtcaaatcg atttctaaca
    42481 atgttttaga agttgaggtg tactattcta gtttcaatct actatacatg taaata
//
LOCUS       DBEROU010000014        41021 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.014, whole genome shotgun sequence.
ACCESSION   DBEROU010000014 DBEROU010000000
VERSION     DBEROU010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 41021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.014"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(109..918)
                     /locus_tag="ACL8CL_000800"
     CDS             complement(109..918)
                     /locus_tag="ACL8CL_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS1330083.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHPRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYITPPSKEMQEWYSHSIKAYRKN"
     gene            complement(920..1723)
                     /locus_tag="ACL8CL_000801"
     CDS             complement(920..1723)
                     /locus_tag="ACL8CL_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS1330084.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            complement(1733..3220)
                     /gene="tacF"
                     /locus_tag="ACL8CL_000802"
     CDS             complement(1733..3220)
                     /gene="tacF"
                     /locus_tag="ACL8CL_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HGS1330085.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYSYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNRIVAPEYYLLTTML
                     SEASLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFVYPVDMVINLP
                     FLINTGLIILLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            3556..4263
                     /locus_tag="ACL8CL_000803"
     CDS             3556..4263
                     /locus_tag="ACL8CL_000803"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HGS1330086.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGDRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIENIIEAIDAYRPL
                     TSEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITGIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            4268..5290
                     /locus_tag="ACL8CL_000804"
     CDS             4268..5290
                     /locus_tag="ACL8CL_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribitol-5-phosphate dehydrogenase"
                     /protein_id="HGS1330087.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            5307..6176
                     /gene="licA"
                     /locus_tag="ACL8CL_000805"
     CDS             5307..6176
                     /gene="licA"
                     /locus_tag="ACL8CL_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HGS1330088.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDATSI
                     KTKFDKIVPILQTIHASGKELRGEFAPFEEIKKYESLIEEKIPYANYEAVREEVFSLE
                     KRLADLGVYRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGADFGYYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            6166..7044
                     /locus_tag="ACL8CL_000806"
     CDS             6166..7044
                     /locus_tag="ACL8CL_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS1330089.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLAFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFTAVANGQL
                     LGLMIVFAAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAVVFLGAPLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            7056..7745
                     /locus_tag="ACL8CL_000807"
     CDS             7056..7745
                     /locus_tag="ACL8CL_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HGS1330090.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            7841..8689
                     /gene="dprA"
                     /locus_tag="ACL8CL_000808"
     CDS             7841..8689
                     /gene="dprA"
                     /locus_tag="ACL8CL_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HGS1330091.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPASILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            9528..10571
                     /locus_tag="ACL8CL_000809"
     CDS             9528..10571
                     /locus_tag="ACL8CL_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HGS1330092.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEYFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            10738..12825
                     /gene="topA"
                     /locus_tag="ACL8CL_000810"
     CDS             10738..12825
                     /gene="topA"
                     /locus_tag="ACL8CL_000810"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HGS1330093.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQNGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCRHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            13046..13405
                     /locus_tag="ACL8CL_000811"
     CDS             13046..13405
                     /locus_tag="ACL8CL_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HGS1330094.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQAYVADFRETKSIARERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            13498..14130
                     /locus_tag="ACL8CL_000812"
     CDS             13498..14130
                     /locus_tag="ACL8CL_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HGS1330095.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGALTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQPEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILKYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            14174..14830
                     /locus_tag="ACL8CL_000813"
     CDS             14174..14830
                     /locus_tag="ACL8CL_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HGS1330096.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVYMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            14900..15943
                     /locus_tag="ACL8CL_000814"
                     /pseudo
     CDS             14900..15943
                     /locus_tag="ACL8CL_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261078.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
     gene            15955..16992
                     /gene="leuB"
                     /locus_tag="ACL8CL_000815"
     CDS             15955..16992
                     /gene="leuB"
                     /locus_tag="ACL8CL_000815"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HGS1330097.1"
                     /translation="MAKKIVALAGDGIGPEIMEAGLEVLEALAEKTGFDYEIDRRPFG
                     GADIDAAGPPLPDETLKASREADAILLAAIGSPQYDGAVVRPEQGLMALRKELNLYAN
                     IRPVKIFDSLKHLSPLKLERIAGVDFVVVRELTGGIYFGDYILEERNARDINDYSYEE
                     VERIIRKAFEIARNRRKIVTSIDKQNVLATSKLWRKVAEEVAQDFPDVTLEHQLVDSA
                     AMLMITNPAKFDVIVTENLFGDILSDESSVLSGTLEVMPSASHSENGPSLYEPIHGSA
                     PDIAGQGIANPTSMILSVAMMLRDSFGRYEDAERIKRAVETSLAAGILTRDIGGQAST
                     KEMTEAIIARL"
     gene            16989..17258
                     /locus_tag="ACL8CL_000816"
     CDS             16989..17258
                     /locus_tag="ACL8CL_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HGS1330098.1"
                     /translation="MKLDEKIALVLLIWNVIIFLIYGIDKFKARRRAWRIQEKILLIL
                     ALICGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYFIWR"
     gene            17262..17621
                     /locus_tag="ACL8CL_000817"
     CDS             17262..17621
                     /locus_tag="ACL8CL_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HGS1330099.1"
                     /translation="MAGSSREHAAWVLADYGFKVVIAGSFGDIHYNNELNNGLLPIVQ
                     PREVREKLAQLKPIDQATVDLEQQKIISPVEEFTFEIDSEWKHKLLNTLDDIGITLQY
                     EELIAAYEKQRPAYWQD"
     gene            17783..18353
                     /locus_tag="ACL8CL_000818"
                     /pseudo
     CDS             17783..18353
                     /locus_tag="ACL8CL_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004915491.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
     gene            18447..18512
                     /locus_tag="ACL8CL_000819"
                     /pseudo
     CDS             18447..18512
                     /locus_tag="ACL8CL_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849983.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
     gene            18675..20606
                     /locus_tag="ACL8CL_000820"
     CDS             18675..20606
                     /locus_tag="ACL8CL_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HGS1330100.1"
                     /translation="MNKRKVSLEDFYKWYSLNKEELLNKATVGEKFNDKLKEEFLQEW
                     PLDRILTMSIDEYVIGKGQQNKSLCYALEKGKYKNLFLGISGGSASKFGIYWNKKTNK
                     YKDQANNEISELDQRFSKLKSDLYEIIKEGIRFNFENPIFDMKRSTNEFIGRSAMVTK
                     LLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPDYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNEGDENDDADY"
     gene            20593..21909
                     /locus_tag="ACL8CL_000821"
     CDS             20593..21909
                     /locus_tag="ACL8CL_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HGS1330101.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKDQKIFETVNQKIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYQRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGAQLKKWSIRIPQNASFYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            22075..23061
                     /locus_tag="ACL8CL_000822"
     CDS             22075..23061
                     /locus_tag="ACL8CL_000822"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HGS1330102.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            23125..23196
                     /locus_tag="ACL8CL_000823"
     tRNA            23125..23196
                     /locus_tag="ACL8CL_000823"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23158..23160,aa:Arg,seq:ccg)
     gene            23379..24077
                     /gene="rnc"
                     /locus_tag="ACL8CL_000824"
     CDS             23379..24077
                     /gene="rnc"
                     /locus_tag="ACL8CL_000824"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HGS1330103.1"
                     /translation="MKELQTVLKNHFEIEFADKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            24068..27607
                     /gene="smc"
                     /locus_tag="ACL8CL_000825"
     CDS             24068..27607
                     /gene="smc"
                     /locus_tag="ACL8CL_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HGS1330104.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKYDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLATAKEKASQQKDELETAKVQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDSLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDVYYQTALEIALGASSQHIIVEDEESATKAIDFLKR
                     NRVGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHAREAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAADEASLGSEEAALKTLQDQMAALTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            27604..28398
                     /locus_tag="ACL8CL_000826"
     CDS             27604..28398
                     /locus_tag="ACL8CL_000826"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1330105.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYV
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNEHVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPVAPENARPEILELAKTVIGHHKERSVIAYMEGL"
     gene            28398..29216
                     /locus_tag="ACL8CL_000827"
     CDS             28398..29216
                     /locus_tag="ACL8CL_000827"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1330106.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKETLKAARDRGIKVVLTT
                     GRPIKAMDFFLHELGTDGQEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFEDLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAAANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            29220..30497
                     /gene="ftsY"
                     /locus_tag="ACL8CL_000828"
     CDS             29220..30497
                     /gene="ftsY"
                     /locus_tag="ACL8CL_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HGS1330107.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLETIE
                     ENNSEVLEPERPQAEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKQAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEAPHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(30543..32030)
                     /gene="zwf"
                     /locus_tag="ACL8CL_000829"
     CDS             complement(30543..32030)
                     /gene="zwf"
                     /locus_tag="ACL8CL_000829"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HGS1330108.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYKSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATANKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(32179..32919)
                     /locus_tag="ACL8CL_000830"
     CDS             complement(32179..32919)
                     /locus_tag="ACL8CL_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1330109.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEKLGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            complement(32919..35084)
                     /locus_tag="ACL8CL_000831"
     CDS             complement(32919..35084)
                     /locus_tag="ACL8CL_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGS1330110.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALSEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            35257..36210
                     /locus_tag="ACL8CL_000832"
     CDS             35257..36210
                     /locus_tag="ACL8CL_000832"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HGS1330111.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLATYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            36287..38275
                     /gene="uvrB"
                     /locus_tag="ACL8CL_000833"
     CDS             36287..38275
                     /gene="uvrB"
                     /locus_tag="ACL8CL_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HGS1330112.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAVAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTMGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            38285..38848
                     /locus_tag="ACL8CL_000834"
     CDS             38285..38848
                     /locus_tag="ACL8CL_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS1330113.1"
                     /translation="MIYLRKLKKEDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKETRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNLGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            38814..39302
                     /locus_tag="ACL8CL_000835"
     CDS             38814..39302
                     /locus_tag="ACL8CL_000835"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1330114.1"
                     /translation="MTVIIKSMETPEEIEGKSLVHWQTWREAYDDLLPAEFQETMTLE
                     RCRFFSQKYPENTLIAIGDLKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
     gene            39376..39843
                     /locus_tag="ACL8CL_000836"
     CDS             39376..39843
                     /locus_tag="ACL8CL_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGS1330115.1"
                     /translation="MFRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
     gene            complement(39840..40355)
                     /locus_tag="ACL8CL_000837"
     CDS             complement(39840..40355)
                     /locus_tag="ACL8CL_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HGS1330116.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(40367..40894)
                     /locus_tag="ACL8CL_000838"
     CDS             complement(40367..40894)
                     /locus_tag="ACL8CL_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1330117.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
BASE COUNT        12787 a         7159 c         8926 g        12149 t
ORIGIN      
        1 aaaaaccttg atatgatgcg ttttattgtg ggaatatgta tttcattttc tactaaaatt
       61 atgtttttga ataacctcta tcttagtagt ttgtataatc cccctcaatc agtttttgcg
      121 ataagcttta atgctatgac tataccactc ttgcatttct tttgatggtg gtgttatata
      181 atcgccatac atctgggtta aaaattggtc atattttttg ggaacaggca acatacggcc
      241 ctcaaactca gttaaaatca gttctttaaa ggtatcaact gggaagattt ctttcatccc
      301 ttccttaccg atcccaactc ctccttcata ttgaggagtg ttggttacag catttttgac
      361 tagttgatca attttcttgt aaaagtagcg aggattgaca aaccggagag cgtaccagct
      421 acataatcta agaaaatctt ttagtttgct atcaccgtga actgctcgtg attttttgat
      481 ataagctagt tgacgaaggg ctacatactt atagctcttg tcgacaatgc tcaagtctgt
      541 aaatcgatca attgggaaga catcgatgaa aaggctggta tcatgacgct tgtacttaac
      601 atggtcttct ataacagtag aagtgtccaa aatcgatgcg aaattatgga agtaccaaga
      661 agatgtatcg taggaaagaa ccttgtagcg agggtgattt tcttcttcaa taatcttcag
      721 taaacgctca taatcctcac gataaaggga aatatcaata tcatcatccc aaggaatcat
      781 acctttgtgg cggatggctc caagcatggt tccataactg agaaaataag gaatatcatg
      841 tttcttacaa gtctcatcaa tatagtccag cagggctagt tgaatttctt taatttcttt
      901 tttttctaaa tattgcatcc taatcctcca atttataagc gtgaaattca tgactgtaga
      961 agcgtttttc ttctggtggt agggtcatat aatctccata aaattgtgtc aaaatagtat
     1021 caaatttttc aggtgcagga aggcttaaat tctcaaaggg taaatcgatt gttttatcaa
     1081 aggtaccact tgggaagact tccttttcct taaattttga aggaataaaa gccatatact
     1141 gcccattttc acgactatat ttttgaattt ctttctcgat tttatttgca aaataacgag
     1201 gagaaaccgg tcgaaggagc aaccaaaagg ctgttcgtat ccaatctttt aaaaggctat
     1261 ccttatagac aatattttta tgtttactga aagacagcag tttgaagctt tccagtttat
     1321 aacaagtatc aatgacctta ggatcatcaa agcgatctat agggaaaata tcgataaaga
     1381 taccagactc ataggttttt gtatttcgag tatcaataat tttagtcgta ctgtcggtta
     1441 tcttgataaa gttgttaaag tagttcttat cagtttctaa ggataggagc ttatacttgc
     1501 ttttttcctt ttgaaaaatg ttaataaatc gttggtagtc ttctctaggc atggataaat
     1561 caatatcgtc gtcccaaggg ataaagccct catgtcgaac cgccccaatc agagtaccgt
     1621 agttaataat atagttgata ttgtgctttt tacagagagt atcaatataa tccaaaattt
     1681 ctaattcaat ttgtttggca tcttcaacgg ttagttgttt cattttaaac tcctatgatt
     1741 ttttaaattt attttttaag gctagaacat ggtttaaaaa ttcatagaaa atgctatctt
     1801 ttgtgaagac aagtagacta atataagaga tagctgatag caagataatc aaaccagtat
     1861 taatcaaaaa tggcaaatta atgaccatat ccacaggata cacgaaatta atcaggaaat
     1921 aaattgctac aaaggaaagt gaaaagagag agtatcgaac agtatagcta aagatatgtc
     1981 ccaagtggat gagttgtttt ctatggatga aaatgatata gaaaacaagt agagaagcct
     2041 ctgataacat agttgtcagt aagtagtatt cgggagccac gatacggttg aaaaagagga
     2101 gactattcaa gcccaaattg agtagcccag caaagactgt ataaactgtg atacgttttt
     2161 catagccatt ggtaaagaga atttgggaac caagaatggt atctaaggcc aggataatcg
     2221 tacgaaaagc gaagagagag gtcaagatgc cgcctccgat atatttttca ctaccgtaaa
     2281 gtaggatggc atttggtcct aaaaccatga gtccaaaact cagtggaatg ataaagaagt
     2341 taaagattcg actacctcta ttaaccagag aaacataggc ttctttgtct cctttcccca
     2401 gatagtaact gagacgaggc acactcactc caattgcacc tgttacaacc ccagctataa
     2461 cggtcacaat tcgctgagct atggtatagt aactaacgtt gacatcaatc cctgttttaa
     2521 cgaggaagag gcgatctaaa aaagtgaaga gcatattggc attggcaaag actaacatgg
     2581 ctgtcagagg gagaaagagt ggtttaaaat cacttaggtg aatcttaaca agtttgatgt
     2641 ctcttttaat ccaaaaataa ctaatcaggt agttaatcag cgtcgataaa ctcatcacaa
     2701 gtgtatagac aacaatatcg tgttcatttt taacaaataa gaaaatagag accagcatca
     2761 ggatacggat gaaggcagtt ttgtaaaaga gaaaactgta attttccaga gcttcattga
     2821 cccattcgat tgaaaaaatc tgggcaatga gttgaatccc cataacaagg tagacctttt
     2881 tgacgattgg attatcagta aagaagagag gataggctag gatatagaca gcagtggtca
     2941 aaatcgtaca agcgatgcac aaataaaaaa gactagaaaa ggttctgtta agatcttttt
     3001 tgttatcctt gacattactg atagccctta aaccgtagct atagacacca taagttgcaa
     3061 agggcaagaa aaatgacaaa atagtgtcga ctgagttgaa gtaaccatag tcagttcggt
     3121 ccaagacacg cgcgacatag gttccagtta ggatgggaaa aataatattc aagacacgaa
     3181 ttcccatgta agatagagca tttaatttta tacttttcat tcaatttacc tcgtttttca
     3241 ttatatcata aagttagcta ataagaaatg aagggcagta agccaagcaa tcactttgaa
     3301 gtttcaaatc ttaaatttta agttttcttt aaggaaagta tattattctg aaggactcta
     3361 aaatttcgca gtcatttatt agtaattgct acagaattcc tagtcattac tagaaatgga
     3421 ctagtttctt tgaataatag aactgcataa ttctcctatt ctagaagggg aggaccagta
     3481 tttcttttat gataggacta gattgtggta taatagagag aataagtttt tttagtaaga
     3541 caaaggagaa aatagatgat ttatgcagga attcttgccg gtggaactgg cacacgcatg
     3601 gggatcagta acttgccaaa acaattttta gagctaggtg atcgacctat tttgattcat
     3661 acaattgaaa aatttgtctt ggagccaagt attgaaaaaa ttgtagttgg tgttcatgga
     3721 gactgggttt ctcatgcaga agatcttgta gataaatatc ttcctcttta taaggaacgt
     3781 atcatcatta caaagggtgg tgctgaccgc aatacaagta ttgagaacat cattgaagcc
     3841 attgatgctt atcgtccgct tacttcagag gatatcgttg ttacccacga ttctgttcgt
     3901 ccatttatta cacttcgcat gattcaggac aatatccaac ttgcccaaaa tcatgacgca
     3961 gtggacacag tggtagaagc agtggatact atcgttgaaa gtaccaatgg gcaattcatt
     4021 acaggtattc caaatcgtgc tcacctctat cagggacaaa cacctcaaac attccgttgc
     4081 aaggacttca tggaccttta tggatctctt tctgatgaag agaaggaaat cttgacagat
     4141 gcatgtaaaa tctttgtgat caaaggaaaa gatgtggcct tggccaaagg tgaatactca
     4201 aatctgaaga ttacaaccgt aacagatttg aagattgcaa aaagtatgat tgagaaagac
     4261 tagtaaaatg attaatcaaa tttatcaact aactaagcct aagtttatca atgtcaaata
     4321 tcaggaagag gctattgacc aagagaatca tatccttatc cgtcccaact acatggctgt
     4381 ctgtcatgcg gatcagcgtt actatcaggg aaaacgtgat cccaagattt tgaataaaaa
     4441 gcttccaatg gcaatgattc acgagtcatg tggaaccgtc atttctgacc cgaccggaac
     4501 ctacgaggtt ggtcaaaaag ttgtcatgat tcccaatcag tctcctatgc agagtgatga
     4561 agaattctat gaaaactaca tgacagggac ccatttcttg tctagtggat ttgatggctt
     4621 tatgagagag tttgtttctc tccctaaaga tcgtgtggtg gcttatgatg ctattgaaga
     4681 tacggttgca gccattacag agtttgtcag tgtgggcatg cacgctatga atcgtctatt
     4741 gactcttgct catagcaagc gggagcggat cgccgttatt ggagatggaa gtttagcttt
     4801 tgtggttgcc aatattatca actatacttt gccagaatca gagattgtgg ttattggtcg
     4861 tcattgggaa aagttggaac tcttctcatt tgccaaagaa tgctatatta cggataatat
     4921 tcctgaagat ttggcctttg accatgcttt tgaatgttgt ggtggtgatg gtactggacc
     4981 agctattaat gacttgattc gctacattcg tcctcaggga acgattctca tgatgggagt
     5041 tagcgaatat aaagtcaatc tcaatactcg cgatgcctta gaaaagggct tgattttggt
     5101 tgggtcatct cgttctggtc gcattgattt tgaaaatgct atccaaatga tggaagtcaa
     5161 gaaatttgcc aatcgtctta aaaatatcct ttatctagaa gaacctgtaa gagaaattaa
     5221 agatattcat cgtgtctttg caaccgattt aaacacagcc tttaaaacag tgtttaagtg
     5281 ggaagtataa gtactggagg ttaattgtgg agaaaatcat taaagaaaaa atttcttcct
     5341 tacttagtca agaagaggaa gtcctcagtg ttgaacaact gggtggaatg accaatcaaa
     5401 actatttggc caaaacaaca aataagcaat acattgttaa attctttggt aaagggacag
     5461 aaaagcttat caatcgacaa gatgaaaagt acaatcttga actactaaag gatttaggct
     5521 tagatgtaaa aaattatctt tttgatattg aagctggtat caaagtaaat gagtatatcg
     5581 aatctgctat tacgcttgat gcgacttcaa ttaagaccaa atttgataaa attgttccaa
     5641 tattacaaac tattcatgct tctggcaagg aattaagagg agaatttgct ccttttgaag
     5701 aaatcaaaaa atatgaatcc ttaattgaag agaaaatccc ttatgccaac tatgaagctg
     5761 ttcgagaaga agtcttctcc ttagagaaaa gactggctga cttaggtgtt tacagaaaat
     5821 cttgtcatat cgatttggtg cctgaaaact ttatcgaatc acctcaagga cgactttatt
     5881 tgattgactg ggaatattca tcaatgaatg atccaatgtg ggatttggct gccctctttt
     5941 tagagtctga attcacttcc caagaggaag aaactttctt atctcactat gagagtgacc
     6001 aaacaccggt ttctcatgaa aagattgcta tttataaaat tttacaagat actatttgga
     6061 gtctatggac tgtctataag gaagagcaag gtgcagattt tggttactat ggtgtgaatc
     6121 gttaccaaag agctgttaaa ggtttggctt cttatggagg ttcagatgaa aagtaaaaac
     6181 ggagttcctt ttggccttct ctcaggtatt ttctggggct tgggtctaac ggttagtgct
     6241 tatatctttt cgatttttac agatttgtca ccctttgtgg tggctgcagc tcatgatttt
     6301 ttgagcatct ttatcttact agcttttctc ttggtaaaag aagggaaagt tcgcctctca
     6361 attttcttaa atattcgcaa tgtcagtgtt atcatcggag ccttgctagc aggccctatc
     6421 ggtatgcagg ccaatcttta tgcagttaag tatatcggaa gttctttagc ttcatctgta
     6481 tcggctattt accctgcgat ttcagttcta ttggctttct tctttttgaa gcacaagatt
     6541 tcgaaaaata ctgtatttgg gattgtcttg attattggag ggattattgc ccagacctat
     6601 aaggttgaac aggttaattc tttctacatt gggattcttt gtgctttggt ttgtgctatt
     6661 gcatggggaa gtgagagtgt tcttagctct tttgccatgg aaagtgaatt gagtgaaatc
     6721 gaagccctct taatccgtca agtaacttcg ttcttgtcct atcttgtgat tgtgctcttc
     6781 tctcatcagt catttactgc agtagccaat ggacaattgc taggtctcat gattgttttt
     6841 gcagcctttg atatgatttc ctacttggct tattatatcg ctatcaatcg cttgcaacca
     6901 gccaaggcta caggcttgaa cgtgagctat gtagtatgga cggtcttgtt tgcagttgtt
     6961 ttcttgggtg caccgctaga tatgctgacc attatgacgt cacttgtcgt cattgctgga
     7021 gtttatatta ttattaaaga ataaaggaga ttcgtgtgaa agccattatc ttagcagcgg
     7081 gattgggaac tcgcttgcgt cctatgactg aaaatacccc taaagccttg gttcaggtta
     7141 atcaaaaacc cttaattgag taccaaattg agtttctcaa agaaaaagga atcaatgaca
     7201 tcatcatcat tgttggttat cttaaagaac aattcgatta cttgaaagag aaatacggtg
     7261 ttcgtctcgt tttcaatgat aaatacgctg actacaataa cttttactct ctctatcttg
     7321 taaaagaaga attggccaac agctatgtta ttgatgcaga taactatctc tttaaaaata
     7381 tgttccgcaa tgatttgact cgttcgactt attttagtgt ttatcgtgaa gattgtacca
     7441 acgaatggtt cttggtctat ggagatgact acaaggttca agacattatt gttgatagca
     7501 aggcaggtcg catccttagt ggtgtatcct tctgggatgc tccaactgca gaaaagattg
     7561 tcagctttat cgataaggct tatgcaagtg gcgaatttgt tgatctctac tgggacaata
     7621 tggttaagga taatatcaaa gagctagatg tctatgttga agaattagaa ggcaatagca
     7681 tttatgagat cgatagtgtc caagactatc gtaaattaga agaaattctt aaaaacgaaa
     7741 attaaagatt ccaacatctg acaaaatagt cggatgtttt ttgatttttt acgaactttt
     7801 acgaatagat agatgagtag aaaaagaaat ggagttattt atgaaaatca caaactatga
     7861 aatctataag ttaaaaaaat caggtttgac caatcaacag attttgaaag tgctagaata
     7921 cggtgaaaat gttgatcagg agcttttgtt gggtgatatt gcagatatat caggttgccg
     7981 taatccagcc gtttttatgg aacgttattt tcagatagac gatgcgcatt tgtcgaaaga
     8041 gtttcaaaaa tttccatctt tctctatttt agatgactgt tatccttggg atttgagtga
     8101 aatatatgat gcgcctgtac ttttatttta caagggaaat cttgacctcc tgaaattccc
     8161 gaaggtagcg gtcgtgggca gtcgtgcttg tagcaaacag ggagctaaat cagttgaaaa
     8221 agtcattcaa ggcttggaaa atgaactggt tattgtcagt ggtctggcca agggcattga
     8281 cacagcagct catatggcag ctcttcagaa tggcggaaaa accattgcag tgattggaac
     8341 aggactggat gtgttttatc ctaaagccaa taaacgcttg caagactaca tcggcaatga
     8401 ccatctggtt ttaagtgaat atggacctgg cgaacaacct ctgaaatttc attttcctgc
     8461 ccgtaatcgc atcattgctg gactttgtcg tggtgtgatt gtagcagagg ctaagatgcg
     8521 ttcaggtagt ctcattacgt gtgagcgagc aatggaagaa ggacgcgatg tctttgctat
     8581 tcctgctagc attttagatg gactatcaga cggttgccat catttgattc aagaaggagc
     8641 aaaattggtc accagtgggc aagatgttct tgcggaattt gaattttaaa aatgacctaa
     8701 gctagaattc taagaaaaaa tcaattttaa gagaaaatga acccaacatt tccataataa
     8761 aacgcatatt agcaagtttt taacacttga taatatgcgt tttttctaag tggattagta
     8821 gagtagagga tttttctcat ataatactct tcgaaaatct cttcaaacta cgtcagcttc
     8881 catctgcaac ctcaaaacag tattttgagc aacctgtggc tcgcttccta gtttgcgctt
     8941 tgattttcat tgagtataag ggaaagtata gtgaattgaa ataagatgtg aacaactcta
     9001 tcaggaaagt caaattaatt tatagaaata ttttagcagc caaggtgcac tgttatagat
     9061 tcaatacaca ataatttagt gtaattgaga aaggagaaat gattgtgatt gatgttggct
     9121 aggttatgtt caatgattcc taccgtctca aatcttgtca gtaaggaaaa ataaattctt
     9181 caaaagtaga gattacaagg cttgtttaag aaagaattca aagaccttga caaataaaaa
     9241 taaaatggtt attataaaaa atggtctgaa atagatgatg atacttttcg aaaatctttt
     9301 tacgtcagct cagctttgcc ttgctgtgtt ttgagcaagc tacggttagc ttccgagttt
     9361 gattttcatt tactagaaat gaaactgatg agagatatca gtagacatct gagttaggat
     9421 attatggaaa atgataaaaa gagctcgtga gattggcata tcagactact aaagtattga
     9481 gtttgttagg attttagcga ctagttagct gggaaaggaa gatatttgtg acaaataata
     9541 aactgtattc gttgatagaa tttagaaata aaatatatga agaattagaa ctttccagaa
     9601 gtgatttagc gattttacta tgtgccatgc ttatcgcctc tatcggatta aatatggatt
     9661 cgactcccgt gattattgga gccatgttaa tctctccttt gatgacacct attctgggag
     9721 tggggctctc tctagctata tttgatttta aattgttaag aaaatctttt aaaatattag
     9781 ctattcaaat tcttgccagt ctaatagctt caacacttta tttttatctt tctcccattt
     9841 cgtatgctag ttcggagatt gttgctagaa cctctccgac tatttgggat gttctcattg
     9901 cttttgtagg agggatagca ggtattattg gtgctaggaa aaaagagacc aataatattg
     9961 ttcctggtgt tgctattgca accgccttga tgcctcctct ttgtacagta ggttatgcta
    10021 ttgcttctgc taatctaaaa tttatcatag gctcctctta cctattcctc atcaattgta
    10081 gctttattgt cattgcgact tatataggtg ttaggttgat gatggttaag aaacattatt
    10141 ttaaagataa tgaagaagac tctaaaatgc gtaggatttt gcttctagtt gctgttttgc
    10201 tgatgattcc gagtttcatc tctgcaacga ctttagtgag agaaacgttg aaaaaagagt
    10261 cccttaagaa atttatatca gagcagtttc aggggtataa tattttgaaa aaaacctatt
    10321 ctaaaaagac tcatacccta aagctaacca tttcaggaaa ttatttgaca gaagaagaac
    10381 tcgatatgat ttcaagtaag agaggtgact atggtttaag tgatgtttct gttcaagttt
    10441 cacaattgtc tgattcagaa caacttagta aggaagagct ggtggagtat ttcttccagt
    10501 atatcaagga taaggaagca aaagaaaagg agaaagctaa taaatttgat acagagtctg
    10561 aggagcaata atttcttgag aatagctggt ttctctcgtg agtcttctat gtatatcaaa
    10621 ggaagactga ggttttaagt ataaaacttt tcttctatta tagtagctaa gtcttgacag
    10681 ggttcaaaaa atggtttaca ctttataaag tttattactt tgaaaaggtg tgatactgtg
    10741 gctacggcaa caaaaaagaa aaaatcaaca gttaaaaaaa atctagtcat cgtggagtcg
    10801 cctgctaagg ccaagacgat tgaaaaatat ctaggcagaa actacaaggt tttagccagt
    10861 gtcgggcata tccgtgattt gaagaaatcc agtatgtccg tcgatattga aaataattat
    10921 gaaccgcaat atatcaatat ccgaggaaaa ggccctctta tcaatgactt gaaaaaagaa
    10981 gctaaaaaag ctaataaagt ttttctcgcg agtgacccgg accgtgaagg agaagcgatt
    11041 tcttggcatt tggcccatat tctcaacttg gatgaaaatg atgccaaccg tgtggtcttc
    11101 aatgaaatca ccaaggatgc agtcaaaaat gcttttaaag aacctcgtaa gatcgatatg
    11161 gacttggtcg atgcccaaca agctcgtcgg atcttggatc gcttggtagg gtattcgatt
    11221 tcgcctattt tgtggaagaa ggtcaagaag ggcttgtcag caggtcgcgt tcagtccatt
    11281 gcccttaaac tcatcattga ccgtgaaaat gaaatcaatg ccttccagcc agaagaatac
    11341 tggacagttg atgctgtctt taaaaaggga accaaacaat ttcatgcttc cttctatgga
    11401 gtagatggta aaaagatgaa actgaccagc aatgacgaag tcaaggaagt cttgtctcgt
    11461 ctgacgagta aagacttttc agtagatcag gtggataaga aagagcgcaa gcgcaatgct
    11521 cctttaccct ataccacttc atctatgcag atggatgctg ccaataaaat caatttccgt
    11581 actcgaaaaa ccatgatggt tgcccaacag ctctatgaag gaattaatat cggttctggt
    11641 gttcaaggtt tgattaccta tatgcgtacc gattcgactc gtatcagtcc tgtagcgcaa
    11701 aatgaggcgg caagcttcat tacggatcgt tttggtagta agtattctaa gcacggtagc
    11761 aaggtcaaaa atgcatcagg tgctcaggat gcccatgagg ctattcgtcc gtcaagtgtc
    11821 tttaatacac cagaaagcat cgctaagtat ctggacaagg atcagctcaa gctatatacc
    11881 cttatctgga atcgttttgt ggctagccag atgacagcgg ccgtttttga taccatggct
    11941 gttaaattgt ctcaaaatgg agttcaattt gctgccaatg gtagtcaggt taagtttgat
    12001 ggttatcttg ccatttataa tgattctgac aagaataaga tgttaccgga catggttgtt
    12061 ggagatgtgg tcaaacaggt caatagcaaa ccagagcaac atttcaccca accgcctgcc
    12121 cgttattctg aagcaacact gattaaaacc ttagaggaaa atggggttgg acgtccatca
    12181 acctatgcgc caaccattga aaccattcag aaacgttatt atgttcgcct ggcagccaaa
    12241 cgttttgaac cgacagagtt gggagaaatt gtcaataagc tcatcgttga atatttccca
    12301 gatatcgtaa acgtgacctt cacagctgaa atggaaggta aactggatga tgtcgaagtt
    12361 ggaaaagagc agtggcgacg ggtcattgat gccttttaca aaccattctc taaagaagtt
    12421 gccaaggctg aagaagaaat ggaaaaaatc cagattaagg atgaaccagc tggatttgac
    12481 tgtgaagtgt gtggcagtcc aatggtcatt aaacttggtc gttttggtaa attctacgct
    12541 tgtagcaatt tcccagattg ccgtcatacc caagcaatcg tgaaagagat tggtgttgag
    12601 tgtccaagct gtcatcaggg acaaattatt gagcgaaaaa ccaagcgtaa tcgcctcttc
    12661 tatggttgca atcgctatcc agaatgtgaa tttacctctt gggacaagcc tgttggtcgt
    12721 gactgtccaa aatgtggcaa cttcctcatg gagaaaaaag tccgtggtgg tggcaagcag
    12781 gttgtttgta gcaaaggcga ctacgaggaa gaaaagatta aataagagga ggggcccgaa
    12841 agtaaatttc aggctccttt tggttagaac ttgacaaaat acatcatttt atgcgaaact
    12901 agaagaagat tattttatac tcaatgagaa tcaaagagca aattagaaag ctagtcgcag
    12961 gttgctcaaa gcactacttt gaggttgaag atggaagctg acctggtttg aagagatttt
    13021 cgaagagtat taactaggag aagttatgcg tcttatctat ctgataattg gttttttatc
    13081 actggccttg gctattgttg gggttgtttt acccttgttg ccgacaacac ctttcctttt
    13141 gttgtctatt gcttgtttct ccagaagttc caagcgattc gaagattggc tttatcatac
    13201 caagctctat caagcatatg tagctgattt tcgtgagacc aagtctattg cgcgtgaacg
    13261 aaagaaaaaa atcatcgtct ctatctacgt cttgatggga atttctattt attttgcacc
    13321 tcttttacca gtcaaaatcg gtctgggtgc tttgaccatc tttattactt attatctctt
    13381 caaggtcatt ccagacaaag aatagttaaa acagtagtta tttgccttga taaaattgaa
    13441 agcatattca taacaatatg atataataaa attgaagtaa tattcaagga gaatcaaatg
    13501 atttacgaat tttgtgctga aaatgtgact ttacttgaaa aagcgatgca ggctggagct
    13561 cgtcggattg aactctgtga taatctagca gttggtggga caacacccag ctatggagtg
    13621 actaaggcag cggttgaact ggcagctaac tacgatacaa ccatcatgac catgattcgg
    13681 ccacgtggtg gtgactttgt ctataatgac ctagaaattg ctatcatgct agaagacatt
    13741 cgtttgactg ctcaggctgg aagtcaaggg gttgtatttg gagctttaac tgctgataaa
    13801 aagttggata agcctaatct ggaaaagtta attgctgcat caaaaggaat ggaaattgtc
    13861 tttcacatgg cctttgatga actaagtgat gaagatcaac cggaagctat tgactggctc
    13921 agtcaagccg gtgtcactcg tatcctaact cgtgctggtg tgtctggcga ctccttagaa
    13981 aaacgttttg ttcactatca cagaattttg aagtacgcta aaggtaaaat tgaaattcta
    14041 ccaggtgggg ggattgacct tgaaaaccgt caaaccttta tcgaccaggt gggggtaaca
    14101 caattgcatg gtactaaggt tgttttttaa aaaatagaaa ggaactgcta gctttgggta
    14161 gcagttttca cttatgtttg aaatttttaa atcctatcaa tttaatcaag aaaaggctca
    14221 tgattatggt tttatagaaa atagcgaagt ctggacatat agttgccaga ttttgcaagg
    14281 tgactttgtc atgactgtgt ccatcactgc tgataatgtg aactttcaag tctttgacca
    14341 agagactggt gacctctatc ctcacgttta tatggaaagc atgaggggaa gttttgtcgg
    14401 aaatgtccgt gaggcttgtc tggagattct ttaccagatt cggaaggctt gttttgatgt
    14461 gcaagatttt atctgtcatc agactaagcg tatcatgact caagttcagg aaaagtatgg
    14521 aaaccagttg gagtatctgt gggaaaaatc gcctgataca gctgtattgc gccatgaagg
    14581 caatcaaaag tggtatgccg tcttgatgaa aatctcttgg aataagctgg aaaagggcag
    14641 agaaggacaa gtggaagcag tcaacctcaa gcatgaccaa gtagctaatt tgctttcaca
    14701 aaaggggatt tatccagcct tccatatgag caagcgctac tggattagtg tgtcccttga
    14761 tgatacttta tcagatgaag aagtactgga attgatagaa aaaagttgga acttaacctc
    14821 taaaaaatga aatattttaa taattttcat gaactttcaa ttagctaaat attctttact
    14881 gaagaaattt tcagaaaata taggatttac cacaccagag gaatgtggtg ccatcttcaa
    14941 atacctgatt gagaatgtca agacggatcg tcagatcatc tattcgcctc actgtcatga
    15001 tgacctcgga atggcagtgg caaatagcct tgctgctgtc aagaatggtg caggacgtgt
    15061 tgaagggact atcaatggta ttagggagcg agctgaaaat gctgctttgg aagaaattgc
    15121 agtggctctc aatattcgcc aagattacta ccaagtagaa accagtattg tcctaaatga
    15181 gaccatcaat acgtcagaaa tggtttctcg cttctctggt attccagttc ctaaaaacaa
    15241 agccgtcgtt ggtggcaata ccttctccca cgaatctggt attcaccaag atggagtcct
    15301 taaaaatcct ctcacttatg agatcatcac acctgaattg gttggtgtta agattccgct
    15361 tggaaaatta tctggtcgcc atgcttttgt tgagaaactg agagaattgg ccctagattt
    15421 tacagaagag gatatcaaac cactctttgc taagttcaag gcactggccg ataagaagta
    15481 agaaatcaca gatgcagata ctcgagcttt ggtagctgga accatggttg aaaatccaga
    15541 aggcttccac tttgatgatt tacaacttca aactcatgca gataatgaca ttgaagcgct
    15601 cgttagccta gccaatatgg atggtgagaa agtcgaattt aatgcgacag ggcaaggttc
    15661 cgttgaagca atctttaacg ctatcgataa gttctttaac caatctgttc gtttggtgtc
    15721 ctacactatc aatgcggtaa cagatggaat cgatgcccag gatcgggttt tggtcactgt
    15781 tgaaaacaga gatacagaaa ccatctttaa tgcagcaggg cttgattttg atgtgttgaa
    15841 ggcttctgct attgtctata taaacgctaa tacctttgtt caaaaagaga atgcaggtga
    15901 gatgggacgc agtgtttctt accacgatat gcctagtgtg taaaggagaa ggctatggca
    15961 aagaaaatag tagctctagc aggagacgga attggcccag aaatcatgga ggctggttta
    16021 gaagttctgg aggctctagc tgaaaaaaca ggttttgact atgagattga cagacgaccg
    16081 ttcggaggtg cagatattga tgcagcaggg cctcccttac ctgatgaaac ccttaaggca
    16141 agtagggaag cagatgctat cctactagca gctatcggta gtcctcagta tgatggagca
    16201 gtggttcgcc ctgaacaagg cctgatggct ctccgtaagg aactcaatct ttacgctaat
    16261 attcgtcctg taaaaatctt tgacagtctc aagcatttgt caccactcaa actggaacga
    16321 attgctggtg tagactttgt cgtggtgcgt gaattgacag gcgggattta ctttggagat
    16381 tatattcttg aagagcgcaa tgcgcgtgat atcaacgact atagctatga ggaagtggag
    16441 cggattattc gcaaagcctt tgaaattgca agaaatcgca gaaaaatcgt tactagtatc
    16501 gataagcaaa atgttctagc gacctcaaaa ctctggcgga aagtagctga ggaagtcgca
    16561 caggatttcc cagatgtaac cttggaacac cagctggtag actcagctgc tatgcttatg
    16621 attaccaatc ctgctaagtt tgatgttatt gtaacggaga atctttttgg agatatttta
    16681 tctgatgaat caagcgtctt atctggtaca cttgaggtta tgccatcagc cagtcattct
    16741 gaaaatggac caagtctcta tgaacctatt cacggttcag cacctgatat tgcaggtcaa
    16801 ggaattgcca atcctacttc catgatttta tcagttgcca tgatgttgag agatagtttc
    16861 ggacgttatg aggatgcaga gcgtatcaaa cgtgctgttg agacaagtct ggcggcagga
    16921 attttaacga gagatatagg aggtcaggct tcaacaaagg aaatgacgga agctattatt
    16981 gcaaggttat gaagttagac gaaaaaattg ctctagtcct tttgatttgg aatgtcatca
    17041 ttttcttgat ttatggcatt gacaaattca aggcaaggag aagagcttgg cgcatccaag
    17101 agaaaatctt actcatttta gcccttattt gtggtggttt tggggcctgg ttagctggaa
    17161 tcacttttca ccacaagact cgaaaatggt actttaaaac agtttggttt cttgggatgg
    17221 tgaccacact agtagcctta tattttattt ggaggtaatg gatggcaggg tcttcgaggg
    17281 aacacgctgc ttgggttcta gcggactatg gttttaaggt cgtgattgca ggatctttcg
    17341 gtgacattca ttacaataat gaactcaata atggcctgtt gccaatcgtt cagcctagag
    17401 aggttagaga gaaattagcc cagctaaaac caatcgacca ggcaactgtg gacttggaac
    17461 aacaaaaaat catctcacca gttgaagaat tcaccttcga gatagatagc gagtggaaac
    17521 ataaactcct aaatactttg gatgatatcg gtattacctt gcagtatgaa gagttgattg
    17581 ctgcttatga aaaacaacga ccagcctact ggcaggatta gaaaaaatag aaaaggaaat
    17641 atagtaaact gaaataagat gtaaacaaat gaattagagc ttaacatcca tttccagcaa
    17701 ttttttagaa actacagtgg actattctgg attcaacaca ttataaaatt atgacaaaac
    17761 acattcacaa gaaggctacg acattttaaa aggtgagggc ggatgtatcg tttgccctac
    17821 taaagttggt tacattatca tgaccagtga caaggcagga cttgagcgta agttcgcagc
    17881 caaagaacgt aagcgtaaca aaccaggtgt tgttctctgc ggtagcatgg atgaacttcg
    17941 cgctttagcg caactcaacc cagaaattga agcattctac taaaaacatt gggatgaaga
    18001 tattcttctt ggttgtatcc ttccttggaa accagaagcc tctgaaaaac tcaaagcata
    18061 cggggatggc cgtgaagaac ttatgactga tgtacgtggt actagctgtt ttgttatcaa
    18121 gtttggaaaa gcaggtgaac aattggctgc caagctttgg gaagaaggta aaatggtcta
    18181 cgcctcatct gcttcaatga caaaacgatt gaaactcgct atgagagcaa ggtgtaatgg
    18241 tgtctatggt cgataaggac ggcaaactca tcccagaaca aggaggagca cgttcaactt
    18301 caccagcacc agttgtgatc cgtaaagggc ttgacattga tatagtaaaa tgaagtaaga
    18361 gcaggacaaa ttaatcagga cagtcaaatc gatttctaac aatgttttag aagtagaggt
    18421 gtactattct agtttcaatc cactataaaa tcatgatgca cctgtcagat acttttaact
    18481 catgggacta ccgtcaggtt gagtattatt aggatagaga agaagtctag tgttaaggac
    18541 attacagctc ataatactag gcttttttat agaaattctt ttctttttct ataggatatg
    18601 gtattctatg tagaaaatat atgttaataa gtaatgccaa tatttaaaca tcattagtaa
    18661 aaggagttag attgatgaat aaaagaaaag ttagtttaga agatttttat aaatggtata
    18721 gtctaaataa agaagagtta ttaaataagg caactgttgg tgaaaagttt aatgataaat
    18781 taaaagaaga gtttctccag gaatggcctt tggataggat tttaacaatg tcaatcgatg
    18841 aatatgtaat aggaaaggga cagcaaaata agtctttatg ctacgctctt gagaagggaa
    18901 aatacaaaaa tctatttctt ggaatttctg gtggctcagc ttcaaaattt ggtatttatt
    18961 ggaataaaaa aacaaacaaa tataaagatc aagctaataa tgagatttca gagttggatc
    19021 agcgattttc aaaattaaaa tcagatttgt atgaaattat caaagaaggt attcgtttta
    19081 actttgaaaa tcctattttt gatatgaaaa gatcaacaaa tgaatttatt ggtcgttctg
    19141 ctatggtgac aaaattactt tgtatctatt ctgagggaga tcctttcttt ggtgtaaata
    19201 ttaatagtca gaaagaattt tggaaccact ttgtttctca gacaaatcaa ggtggacctt
    19261 atctgcaaaa tcataaaata attgaactgg tgtccaaaac ttatcctgag ttggagccat
    19321 cgaaattagg aactatgctt tttgagtatt ctaagctttt tatggaaaat aaggaagaca
    19381 atagtacaat ggattcatca aacaattttc gtcatcaatt aactcaatct ctattaaagt
    19441 ctccaaacct tatccttcgt ggtgctcctg gcacaggaaa gacttatctt gctaaagaaa
    19501 ttgccaaaga attaacggat ggcaacgaag accaaatcgg atttgtacaa tttcacccat
    19561 catatgatta tacggatttt gtagaaggtt taagaccagt atcaaatggg gatggagcta
    19621 ttgagtttag gctacaggac ggtattttta aagatttttg tcagaaagca aaagaaaccc
    19681 aattgattgg aggacaagat aattttgatg aggcttggga ttcttactta gaatatataa
    19741 atgttgctga agaaaaagaa tatataacaa aaacatctta cttatctgtt aatagtagac
    19801 aaaatttgtc agtaaattat gatagtggtg ttccaggatg gtcactacct agcaaatatg
    19861 tttacgagtt gtataaagat aaaaattata ataagcaaga atactacaaa agtggtggaa
    19921 aaactgtcct agaaacattg agaaagagat ttggtttgaa agactatgtt tccccaacag
    19981 aaattgatac tgataagaaa ttcgtcttca tcatcgatga aatcaatcgt ggggagattt
    20041 ctaagatttt tggcgaactc tttttctcta tcgaccctga ctatcgtggt gaaaaaggaa
    20101 gtgtttctac ccaatatgca aatctacacg aaactgatga aaagttctat atccccgaaa
    20161 atgtttacat catcggaact atgaatgata ttgatcgttc agtggatacc tttgattttg
    20221 ctatgcgtcg tcgttttcgt tttgttgaag ttactgccga gggtcaagtt ggcatgttgg
    20281 ataaagagct gaatatccat gcagaagaag caaaaattcg tctaagaaac ttgaacgctg
    20341 ctatcgaaaa tgttcaggaa ttaaacagtc attatcatat tggaccaagt tattttctta
    20401 agttgaagga tgtagatttt gactatgaat tactctggtc tgattatatt aagcctctcc
    20461 tagaagacta cttgcgaggt tcttatgatg aggttgaaac tttggaaact ttgaaaaaag
    20521 catttgaact gacaaataat gagcaaaaag atcaggcagt agctgatgac aatgaaggcg
    20581 atgaaaacga tgatgcggat tactgataat caacacaaga ttattaaaga aaaatttgtt
    20641 gaagaatatc ctaaactaag caatcttctt ttagacagaa ccttggaaag tctatcccaa
    20701 gatgaacgta ttttcatttt tccaaatgat ttgactcata ctcctgattt ggataaggac
    20761 caaaagattt ttgaaacagt caatcagaaa atcaagacag ggaacgtgat tggttttctt
    20821 ggatatggtc aggaaagatt aacgatttcc tcacgatttt ctgatgagag taatgaccat
    20881 tttttgcatt atctcttaaa caaggttctt catatcaatc tcactagttt agatgttgct
    20941 ttgtctcgtg aagagaggct ttatcaactt ttgatgtatc tcttccccaa gtatctacaa
    21001 gctgctattc gaaaaggtct ttataaggaa taccagagat tttctcataa cgacagtcat
    21061 gttaagggag tgattgatgt aagaaaccat ctcaagaaaa atcttccttt cacgggaaat
    21121 attgcctaca caacgagaga gttcacctat gataatcccc tcatgcagtt ggtccgtcac
    21181 actattgaat acattaagaa tcagaaaagc attggtcaag gggtactaga taatctctca
    21241 actagtcgtg aaaacgtatc tgaaatcgtg cgtgtaacgc cctcttataa actagctgat
    21301 cgtgctaaga ttactcggag aaatcaatct aaacctatac gtcatgcata ctttcacgag
    21361 tacagaaact tacaagaact ttgtctgatg atcctaaacc aagaaaagca cggtttaggg
    21421 tatcaagatc aaaaaatcta tggtattctc tttgatgttg cctggctttg ggaagagtat
    21481 gtttacacct tgttgccaaa aggttttgta catcccagaa ataaggataa gacggatgga
    21541 atttcagtat tttctgttgg gaaacgaaaa gtatatccag atttttatga cagagaacga
    21601 aagattgttc tagatgcaaa atataaaaaa ctggaattga ctgaaaaagg aatcaaccgt
    21661 gaggacttat tccagctgat ttcctattct tatattttaa aagctgagaa ggctggactt
    21721 gtttttccga gcaaggacaa ggttattgat aatgagatgg gaaacctagc aggttatgga
    21781 gctcaattga agaagtggtc tattcgaatc cctcagaatg cctcattcta tagtgcattt
    21841 tgtaaaatga tggaaaattc agaagaaaat tttaaagcga ttattgatga agaagtgggg
    21901 agaaaataat ttcccctctt tcttttattt ttaacacaag caaaacccga acaatatatt
    21961 ttaattcata aaataatttg acaaaaactg tactttagtt tataataaat taaggaaaag
    22021 gcgtagtgat gcgagagcat aggtaggtca ttacaaaaga aacgagacat cgatatgtta
    22081 aatgaatttc caatttttga ttacgaagat attcaattga ttccaaataa atgtgtcatt
    22141 aaaagccgtg cagaagcgga tacaagtgtc actttaggaa atcacacctt taaactacct
    22201 gttgtgccag cgaatatgca gacgattttg gatgaaaatg tagcagagca actggctaaa
    22261 ggtggttact tctacattat gcaccgtttt gatgaggcag gacgcattcc ttttattaaa
    22321 cgcatgcacg atcaaggact cattgcttct atctctgtcg gtgttaagga ctatgagtat
    22381 gattttgtta gccagctcaa ggctgatgct cctgagtata tcactattga tattgctcat
    22441 ggtcatgcgg atagcgtgat ttctatgatt caacacatca agaaagaatt gccagatacc
    22501 tttgtcattg ctggaaatgt gggaacacca gaagctgtgc gtgaattgga aaatgctggt
    22561 gcggatgcta ctaaggtcgg aatcggtcct ggtaaggttt gtatcaccaa ggttaagact
    22621 ggttttggta caggtggttg gcagttggct gctctacgct ggtgtgccaa ggctgcgcgt
    22681 aaaccgatta tcgctgatgg aggaattcgt actcacggtg atattgccaa gtctatccgc
    22741 ttcggtgcta gcatgatcat gattggttcc ctctttgcag gacacattga aagtccaggg
    22801 aaaacgattg aagtcgatgg tgaacagttc aaagaatatt atggttcagc ctcacaatat
    22861 caaaaaggtg cttacaaaaa tgtggaaggc aaacgtatct tgcttcctgc taaaggtcat
    22921 ctgcaagaca ctttaactga gatggaacaa gaccttcaaa gtgctatttc ttatgcaggt
    22981 ggacgtcagg ttgctgacct taaacacgtt gattatgtga tcgtgaaaaa ctccatctgg
    23041 aatggggatg cttcccacta agacggggtc tttgaccaaa aaacttgtta ttagagcaaa
    23101 tttctgttat aataaaacaa gtttccaccc ttagtgtaat ggatatcacg taagattccg
    23161 gttcttgaga tgggggttcg attccctcag ggtggatgta aacatcctaa aaagccttta
    23221 atagggcttt tttctttatc ctgacccaaa tttgccgcta aaactgagaa tctctgtcta
    23281 aagaattccc aaatgctttc cagtcccgtt ttagagtgac tcagggggct ttttttgata
    23341 taataaaaag gactattatc agttagaaag aagttggtat gaaagaatta caaactgtac
    23401 taaagaatca ttttgaaatc gaatttgcag acaaaaagtt actggaaact gcctttactc
    23461 atacgagtta tgccaatgag caccgcctct taaaaatttc acacaatgaa cgcttggaat
    23521 ttttaggaga cgctgttcta cagttattga tttcagaata tctatataaa aaatatccta
    23581 aaaagcctga aggtgaccta tcaaaactcc gtgctatgat tgtccgtgag gagagtttag
    23641 ctggttttgc gcgtgattgc cagtttgacc agtttatcaa gttgggtaaa ggggaagaaa
    23701 aatctggtgg tcgcaatcgt gacaccattc ttggtgatgc ctttgaagcc tttcttggtg
    23761 cccttctttt ggataaggat gtggcaaagg tcaaggaatt tatctaccaa gttatgattc
    23821 ctaaggtaga agcaggcgaa tttgagatga ttacagacta taaaacccat ctccaagagt
    23881 tgcttcaggt caatggtgat gtggctattc gttatcaggt gatttctgaa acagggcctg
    23941 ctcacgataa ggtctttgat gtagaagttc ttgttgaagg taagagcatc ggtcaaggcc
    24001 aaggtcgttc taagaaatta gcagagcagg aagctgccaa aaatgccgtt gagaaagggc
    24061 tggattcatg tatttaaagg aaatcgaaat tcaggggttc aagtcttttg ctgataagac
    24121 caaggtcgtt tttgaccaag gtgtgacggc agttgttgga cccaatggat ctggaaagtc
    24181 caatattaca gaaagtctgc gttgggcttt gggggagtct agtgtcaaga gtctccgtgg
    24241 gggcaagatg ccggatgtca tctttgctgg aacagaaagt cgcaaaccgc tcaattatgc
    24301 ttctgtagtt gtgactctgg ataatcatga cggatttatc aaggatgcag gtcaagaaat
    24361 cagggtggaa cgccatatct atcgtagtgg agatagcgaa tacaagattg acggcaagaa
    24421 agtccgtctg cgtgatattc atgacctctt cttggatact ggattgggac gagattcctt
    24481 ctctattatt tcccaaggga aggttgagga gatttttaat tccaagcctg aggaacgacg
    24541 agctattttt gaagaagctg ctggagtttt aaaatacaag actcgcagaa aagaaaccga
    24601 gagtaaactg cagcaaactc aggataatct ggaccgctta gaggacatta tctacgagtt
    24661 ggataatcaa atcaagcctc ttgagaagca agctgagaat gcccgtaagt ttttagactt
    24721 ggaaggacaa cgtaaggcta tttatttaga cgttctggtt gctcaaatca aggaaaataa
    24781 ggcagaacta gagtcgacag aagaagagtt ggctcaggtt caagaactct tgatgagtta
    24841 ttaccaaaag cgtgaaaaat tagaagaaga aaatcaaact cttaaaaagc aacgccaaga
    24901 tttacaggct gaaatggcca aagaccaagg cagtttgatg gacttgacta gtctgattag
    24961 tgatttagaa agaaaattag ccctatcgaa actggagtcc gagcaagtgg ccctgaatca
    25021 acaggaggca caagctcgtt tggctgcttt ggaggataag agaaattcac tcagcaaaga
    25081 aaagtatgat aaagaaagct ctttagctct gttagaggga aatctagtcc aaaataatca
    25141 aaaactcaat cgtttagaag ctgaattgct ggctttctca gacgatcctg atcagatgat
    25201 tgagctctta cgtgaacgct ttgtagctct tttacaagaa gaagcggatg tctcaaacca
    25261 gttgacccgt attgagaatg agttggaaaa tagtcgtcag ctttctcaaa aacaagcaga
    25321 tcaactagaa aagctgaaag agcaattagc tacagctaaa gagaaggcta gtcagcaaaa
    25381 agacgagctt gaaactgcca aggtgcaggt tcagaaatta ttggctgact atcaagctat
    25441 tgccaaggag caagaggagc agaaaacttc ctatcaagct caacaaagtc aactctttga
    25501 ccgtctggat agtctcaaaa acaagcaggc cagagctcaa agtttggaaa atatcctgag
    25561 aaatcatagt aacttttatg caggtgttaa gagtgttctc caagaaaaag atcgcctagg
    25621 tgggattatt ggtgcagtca gtgagcatct gacctttgat gtttattatc aaactgccct
    25681 agagattgcc ttaggggcaa gtagccagca tatcatcgta gaagatgaag agtcggcaac
    25741 caaagctatt gatttcctca aacgaaacag agtcggtcgt gcaacctttc ttcctttgac
    25801 cactattaag gcgcgtacga tttctagtca gaaccaagat gctatcgctg taagcccagg
    25861 tttccttggg atggcagatg agttggtgac ttttgatact agactggaag ccattttcaa
    25921 gaacttgcta gctacgacgg ctatctttga taccgtagaa catgcgcgtg aagctgctcg
    25981 acaagttcgt tatcaggttc gtatggtgac attggatggg acagaattac gcacgggtgg
    26041 ttcctatgcg ggtggtgcca atcgccaaaa taacagtatt ttcatcaagc cagaactgga
    26101 gcaattacaa aaagaaattg ctgcagatga agcaagcttg ggttcagaag aagcggcttt
    26161 gaagaccttg caagaccaga tggctgcatt gacagaaaga ttagaagcca tcaaatctca
    26221 aggagagcag gcacgtattc aggagcaagg cttgtccctc gcttatcagc aaactagtca
    26281 gcaagttgaa gaactggaaa ctctttggaa actccaagaa gaggaaatag atcgtctttc
    26341 tgagggagat tggcaagcgg ataaggaaaa atgtcaagag agccttgcta ctatcgccag
    26401 tgaaaagcaa aatctggaag ctgagattga agaaattaag tctaataaaa acgccatcca
    26461 agaacgctat caaaatttgc aggaagaggt ggcgcaagct cgcttgctta agacagaact
    26521 gcaagggcaa aaacgttatg aagtagctga tattgagcgt ttaggcaagg aattggacaa
    26581 tcttaatatc gaacaagaag aaattcagcg catgctccaa gaaaaagttg acaatcttga
    26641 gaaggttgat acagaattgc tcagtcaaca ggcggaagaa tccaaaactc agaaaacaaa
    26701 tctccaacaa ggtttgattc gcaagcagtt tgagttggat gatatagaag gtcaactgga
    26761 tgatattgcc agtcacttgg atcaagctcg ccagcagaat gaggagtgga ttcgcaagca
    26821 aacacgtgct gaagccaaga aagaaaaggt cagcgagcgc ttgcgccatc tacaaaatca
    26881 attaacagac cagtaccaga ttagctatac tgaagcacta gaaaaggcac atgaattgga
    26941 aaacctcaat ctggcagagc aagaggtgca ggatttagag aaggctattc gctcattggg
    27001 acctgtcaac ttggaagcta ttgaccagta cgaagaagtt cacaaccgtc tggactttct
    27061 aaatagtcag cgagatgata ttttgtcagc gaaaaatctg ctccttgaaa ccattacaga
    27121 gatgaatgat gaggtcaagg aacgctttaa atcaaccttt gaagctattc gtgagtcctt
    27181 taaagtgacc ttcaagcaga tgtttggcgg aggtcaggca gacttgatat tgactgaggg
    27241 cgacctttta acagctggtg tggagatttc tgttcaacct ccaggtaaga aaatccagtc
    27301 gcttaacctc atgagtggtg gtgaaaaagc tctatcggct cttgccttgc ttttctccat
    27361 tattcgtgtc aagaccattc cttttgtcat cttggatgag gtggaagctg cgctggatga
    27421 agccaatgtt aaacgttttg gggattacct caaccgtttt gacaaggaca gccagtttat
    27481 cgttgtaacc caccgtaagg gaaccatggc agcggctgat tccatctatg gagtgaccat
    27541 gcaagaatcg ggtgtttcaa agattgtttc agttaagtta aaagatttag aaagtattga
    27601 aggatgacaa ttaaactagt agcaacggat atggacggaa ccttcctaga tgggaatgga
    27661 cgctttgata tggatcgtct caagtctctc ttggcttcct acaaggaaaa agggatttac
    27721 tttgcggtgg cttcgggtcg gggatttctg tctctggaaa aattatttgc tgatgttcgt
    27781 gatgacatta ttttcatcgc ggaaaatggc agtttggtag agtatcaagg tcaggacttg
    27841 tatgaagcga ctatgtctcg tgacttttat ctggcaactt ttgaaaagct gaaaacgtca
    27901 ccttatgtag atatcaataa actgctcttg acgggtaaga agggttcata tgttctagat
    27961 acggttgatg agacctattt gaaagtgagt cagcattata atgaaaatat ccaaaaagta
    28021 gcgagtttgg aagatatcac agatgacatt ttcaaattta caaccaactt cacagaagaa
    28081 acgctagaag ctggtgaagc ttgggttaat gaacatgtcc ctggtgttaa ggccatgaca
    28141 actggctttg aatctattga tattgttctg gactatgtcg ataagggtgt agctattgtt
    28201 gaattagcta aaaaacttgg tatcacaatg gatcaggtca tggcttttgg agacaatctt
    28261 aatgacttac atatgatgca ggttgtggga catcctgtag ctcctgaaaa tgcacgacct
    28321 gaaattttag aattagcaaa gactgtgatt ggtcaccata aggaacggtc ggttatagct
    28381 tatatggagg gcttataatg gcagatataa aattgattgc attggacttg gacgggacct
    28441 tgctgactac tgataaaagg ctgacggatc gtaccaagga aaccttgaaa gctgcgcgtg
    28501 atcgtggtat caaggtcgta ttgacaactg gtcgtcccat aaaagccatg gatttctttc
    28561 tccatgagtt agggactgac ggtcaggaag atgagtatac cattactttt aatggtggat
    28621 tagttcagaa aaatacagga gaaatccttg ataaaacagt cttttcatat gatgatgtgg
    28681 cacgtttgta tgaagaaaca gagaaattat cactgcctct tgatgccatc tcagaaggaa
    28741 cagtttatca aatccaatcg gaccaagaaa gtctttatgc caaattcaat ccagctttga
    28801 cctttgttcc agtggacttt gaagacttat ctagtcaaat gacctacaac aaatgcgtga
    28861 ctgcctttgc tcaagaaccc ttggatgcag ccattcagaa gatttctcca gaattgtttg
    28921 accaatatga aatctttaaa tcacgtgaaa tgttgctaga atggtcacca aagaatgttc
    28981 ataaagcaac aggtttggca aaactaatca gccatcttgg aatcgaccaa agtcaagtga
    29041 tggcttgtgg tgacgaggcc aatgacctct ctatgattga atgggcaggt cttggtgttg
    29101 ctatgcaaaa cgctgttcct gaagtaaagg cagccgcaaa tgtagtgacg ccgatgacca
    29161 acgatgagga agctgtcgcc tgggctatcg aagaatatgt gctaaaggag aactaagata
    29221 tgggattgtt tgaccgtcta ttcggaaaaa aagaagaacc taaaatcgaa gaagttgtaa
    29281 aagaagctct ggaaaatctt gatttgtctg aagatgttga tcctaccttc acagaagttg
    29341 aggaagtttc tcaggaagaa gcagaggttg aaattgttga acaagctgtg ttccaagaag
    29401 aggaaatcca agacacagtt gaagaaagtc tggatttaga gccagttgta gaagtttctc
    29461 aaaaagaagt cgaagaattt ccacactcag aagaagggaa tactgagttt ctagagacta
    29521 tagaagaaaa taattctgaa gttcttgaac cagaaaggcc tcaagcagaa gaaaccgttc
    29581 aggaaaaata tgaccgcagt cttaagaaaa ctcgtacagg tttcggtgcc cgcttgaatg
    29641 ccttctttgc taacttccgc tctgttgacg aagaattttt cgaggaactg gaagaactgc
    29701 tgattatgag tgatgttggt gtccaagtcg cttctaactt aacggaggaa ctacgttacg
    29761 aagccaagct tgaaaatgcc aagaaacctg atgcacttcg tcgtgtcatc attgagaaat
    29821 tggttgagct ttatgaaaag gatggtagct acgatgaaag catccacttc caagataact
    29881 tgacagttat gctctttgtt ggtgtgaatg gtgttgggaa aacaacttct atcggaaaac
    29941 tagcccaccg ctacaaacaa gctggtaaga aggtcatgct ggttgcagca gataccttcc
    30001 gtgcgggtgc agtagctcag ctagctgaat ggggccgacg agtagatgtt ccagtagtaa
    30061 ctggacctga aaaagctgat ccagccagcg tggtctttga tggtatggaa cgtgccgtgg
    30121 ctgaaggtat cgatattctc atgattgata ctgctggtcg tctgcaaaat aaggataacc
    30181 ttatggctga gttggaaaag attggtcgta ttatcaaacg tgttgtgcca gaagcaccac
    30241 atgaaacctt cttggcactt gatgcatcaa caggtcaaaa tgccctagta caggccaaag
    30301 aattttcgaa aatcacacct ttaacgggaa ttgttttgac taagattgat ggaactgctc
    30361 gaggaggtgt ggttctagcc attcgtgaag aactcaatat tcctgtaaaa ttgattggtt
    30421 ttggtgaaaa aatcgatgat attggagagt ttaactcaga aaactttatg aaaggtctct
    30481 tggaaggttt aatctaatca gaagcaaaaa tcctgcaagg cataaacttg caggaaattt
    30541 ttttattcta agcgaccatc ttgacgatag gtgatatctg gttgccaagt ccatttggca
    30601 ccgaattttt caagtaggtc aaagctggct tgaggtccca tgcttccagc tttatagtca
    30661 tgaagtgggg caccattttc agcccagagc ttttcaatac ggtcaatcaa cttccatgac
    30721 gcacaaactt catcccagtg gctaaagtta gttgagttgt tatttaggac atcataaatc
    30781 aatttttcgt atggttctgg agaagcacca gttgcagtcg catctgtacg gtaatcaagt
    30841 gagttaggag ccaagttaaa ttcttctcct acttgcttcc catttaggct aagagagaag
    30901 ccttctgttg gttgaatata gatggtcaaa atatttggag caagtggttc tccaaagata
    30961 gaatccattt gtttaaagac gatgttgaca tgagttcctt tttcagtcag tcgtttacct
    31021 gtacggaaaa agaaaggaac accacggaat cgatcgctgt ctacaaagaa ggcaccagat
    31081 gtaaaggttt cagttgttga ttctggattc acatttggct cgctacgata agagatgtat
    31141 ttcatgccat caatcttacc agagcggtat tgcccacgga taaagtgttc tttgagttct
    31201 tcatcagttg gatgatagag gtttttaaag accttaatct tttcagcacg aatctcgtct
    31261 tttgtgaagc ttgctggttt gtccatggcg aggagcgaaa gaagttgtag agtgtggttt
    31321 tggaccatgt cacggagggc accggattgg tcatagtagc caccacgttc ttctacaccc
    31381 aagcgctccg caaaggtaat ttgaacattg tcgataaaat ccttgttcca aacgttttca
    31441 aaaatcaagt ttgcaaagcg aactgcaaag atgctttgga tcatttcctt accaagataa
    31501 tggtcgatac ggaaaatttg ttcttcgtca aatgttgcta ggagttcgtc attcaacttg
    31561 tttgcagttg cgtaatctgt accaaatggt ttttcaacga tcaagcgctc aaaacctttg
    31621 ccatcgacaa tgttttcaga tttgaggtgt ttggcaatgg ttccaaagaa ctgaggtgcc
    31681 atagacaaga agaagagctt attgtgttca gcttggtatt tttcattaag ctcagcttgt
    31741 aattgacgca aagcaatata atgttccgaa tcattgacat catggctttg atagtagaag
    31801 tggctagcaa attcttgggc ttgctcggta ctatctgcca aatcaaggat ggactcgaca
    31861 actacagatt caaaatattc cttactccaa ggtctacggg cagttccaat aacggcaaag
    31921 tgcttggaaa gattgccgga tttatatagt ctaaaaaggg aagggtagag cttgcgttta
    31981 gccaggtctc cactcgcacc gaaaattgta acaataacct tagatgacat ctagctacct
    32041 aatttctatt tttattccta gtcttgctag gtatgaggaa acctcatttc ataaacttaa
    32101 ttctaacata attttccgaa aaatcaaaaa ttccaatacg aggtagaaaa actgcaagga
    32161 aatccttaca gtttgagttt agacgtttaa gaccttatct aagaactctt tcagacgagg
    32221 gtgttgtggg ttatcaaaga tttggtcagg tgttccgtct tcaaggaact cgccatctgc
    32281 agtaaagata acgcggttgg caacctgacg agcaaatccc atctcatggg ttacgataat
    32341 catggtcatg ccttgctcag ccaattcctt cataacgtta agtacgtctc caaccatctc
    32401 agggtcaagg gcagaagttg gttcatcgaa gagcatgatg tctggattca ttgctaggcc
    32461 acgagctatg gccacacgtt gtttttgacc acctgatagg ctatctggat tggcattagc
    32521 tttatctgct agtccaacct tttcaagcaa ctccattccc aatttctcag cttcttcctt
    32581 agtcatcaac ttgtgctcaa taggagcaaa ggtgatgttg tccaatacag acatatgagg
    32641 gaagaggttg aagtgttgga ataccatgcc gatattttca cggacgtggt caacattggt
    32701 tgttttttca gttaaatcat agccgttcac agtgatgtga ccgctagtga cttcttctaa
    32761 aagattgagg ctacggagga aagttgactt accagaacct gaaggaccga tgatacaaac
    32821 aacatctcct tcatagaact tagtcgtaat tccttttagg acttcatttt ttccatagtg
    32881 cttgtgtaaa tcatttacat caatttttaa ttttgccatt aacgaatcct cttttctaag
    32941 cgtttcgcta gtctagtcaa aagtgtgata attacaagat agaagatagc aaggattgca
    33001 tacatcttga aactttggta gttacgagca atgataatct taccagtttg gaagagttca
    33061 accaaaccga tagcagatac gatagttgta tctttaagag cgataacgaa ttggttgaca
    33121 aagtttggca acatcaattt agttgcttgt ggcaagataa tcttacgcat ggtttttcca
    33181 taagagatac ccaagcttcg gctggcttcc atttggccaa ctggaacggc ctgaatacca
    33241 ccacgaacga tttcagcgat ataagcagcc gcattgagtg agagggcaat ggttccagct
    33301 acaaagtcgt taattgggct ttgttggcct gtgatagact cgatgaagtt tggaattccc
    33361 cagaagatga aggctgcaag aatcatcaat ggaataccac gaataacgtc aacgaaaatc
    33421 tcagagatga cgcgaagaga tttgtatggg ctaacgctaa acataccgaa gataattccg
    33481 atgacaatgg caatagcaaa tgagataaga gctagagcaa gagtgatacc aagaccgcta
    33541 aggagttgtt tgtagttgtt ttgaagcaag ccccagagcg ttgtttcgtc aacagtactt
    33601 gttgaagcag ttgaagattc gctagctagg tatttgtcaa gaatcttttg gaattcaccg
    33661 tttgctttaa ggtttgcaag tccgttgttg aacatttcaa tcagttctgg atttgctcct
    33721 tttttaacgg caaaggctgt ttcaccgatt ggagttccag agattggagt tttcaatttt
    33781 tgaccttggc tgatagaata tttgagaaca ggttcatcat ccataacggc atcaatggca
    33841 ccagtgttta aactgtcata cattgaagaa ccatcagcaa aggttttgat tttgtagccg
    33901 tatttgcttt gattttctgt taggaaggtt tgagaagcag ttccgttttt aacaccgact
    33961 gtctttcctt ttagatcttc ataagaagca atattacttg attctttgac accaagaatg
    34021 gtattagcag tgtagtatga ttctgagaag tcaaaagttg ccttacgagc atctgtgaca
    34081 gacataccag cgatgatacc atcggcttga ccagcttgga cagcactgat agcagcatca
    34141 aaaccagggt tggtgatttc aatttcaaaa ccttggtctt tagcgattgc cttaatcaat
    34201 tccatatcaa taccagtgta ttggttgctt gaattttgga aaacaaaagg ggcaaaagaa
    34261 gaatcgctgg caatgatata tttagcctta acaggaatag cttttaatcc tgcgagagta
    34321 gttgtagttg gcactgctga agatgatgaa gcagtccatt tcttgataat tttatcaaga
    34381 ctaccatctt ttttcatttc agacaaggct tggttaaatt cagtaaccag gtgctcgtat
    34441 ttacttcctt ttttcacacc gaaagcaaaa cttcctacag cttcaccatc catttcaata
    34501 tggaggtctt gaccttggtt aatggcatat tcgataacag gtttgtcatc catcatggca
    34561 tcgatggcac cagcactcaa gctgttgttc attaaatcac cagtgtcaaa tgttttaata
    34621 gtaaagccat atttatcttt gattgtttca aggaaacgtt gagcggcagt tccgttttta
    34681 acaccaacgg ttttgccagt taattggtcg tacttgctaa ttttgtgtga ctttgtagta
    34741 gcaatgacaa cttttgtatc atagtaagta tcagacatgg tgaagacttt ttcacgttct
    34801 ttagtctttg tcatccctgc catgatagcg tcggcttgcc cagcttgaac cgcattgact
    34861 gctgcgtcaa atccaggata ggacatctga atgttccagc ctttaatctc agcgactttg
    34921 ttaataatgt caacatcaat tcctttataa gtttgatctg aatctttaaa ctcaaaaggt
    34981 gcataggcgg tatcagaaac aatcttaatc gtttctgctt tggcaatacc taatgagaaa
    35041 attgggaata aaattagcaa aaatgctaga aattttttct tcatttttag tctccttttc
    35101 cgaatattct ctcattatat cagaaaaagt aaaaaaaggc aaattgttat atttttcgtg
    35161 tcagaaaata taacattgat ttttcctttt ctttcttgaa ttgctattaa aaagtgctat
    35221 aataatagta tatatagaag aaaagaggac ggggatatga agaacaaaag aatatttaaa
    35281 gacttccaag cttcaaaaat gagtttaaac atttacacaa gccccttgtt agcctttgtt
    35341 tttgtcttca taggagagtt tgtggctttt actttgtatg gtattggctt gttagctctc
    35401 atcggacttg ctagaaattt tggagaggct ggtcaaaatc ttgcaaccta cttgcagacc
    35461 ttgcatcaga gcttgacgga taaaacaagt gactttcgtt taattttagg attactggcc
    35521 tttggtttta ttcttaacac tgtgttcaga tggacaagaa aagttgagaa aagacctatt
    35581 cgaaccttgg gattttatag agagaatttc ctcagcaatc ttctgaaagg atttagtcta
    35641 ggcttggcac tttttcttct gaccttgtta ggtttagtgg tcttaggtca atatcgtttg
    35701 gaatccattc acttgaatcc ttattctctt gcctttgtcg tctttactat cccattttgg
    35761 attttacagg ggacagcaga agaagtggtg gcccgtgctt ggctacttcc tcaattggcc
    35821 tcaagaacca atctaaaact agctattctt atatctagcc tgttctttac cctgcttcat
    35881 atgggcaatt ctggcctcac ccctctatct ctagtaaatc tctttttatt cggagttgcc
    35941 atggctcttt atcttctcaa aactgataca gtttggggtg ttgcaggtat tcatggtgct
    36001 tggaattttg ctcagggtaa tctctttggg attttagtta gtggtcaacc gtcaggaacg
    36061 tctctgatga cctttttacc acaaggcaat caagattggc tatcaggtgg ttcttttggc
    36121 atagaaggtt ccattatgac aagtctggta ttactactgc tgattgtcta tcttgctaat
    36181 aaattaaaga aagaaaatga aaggatgtga cttcggtccg tccttttctt cgtgaaaata
    36241 ctataagtat gctaaaatag gaatagcaca tggagagagg attcttatga tcaatcacat
    36301 tacagataat caatttaaac tagtatcaaa atatcaacca tcaggagatc aaccccaagc
    36361 tatcgagcag ttggtggata acattgaggg gggagaaaaa gctcagattc tgatgggggc
    36421 gactggaaca gggaagacct atactatgag tcaggtcatt tctaaagtca ataaaccaac
    36481 tctggttatt gcccacaata aaactctggc tggtcagctc tatggggagt ttaaggaatt
    36541 tttccctgaa aatgcagttg agtatttcgt atcctactat gattattacc agccagaggc
    36601 ctatgtccct tctagcgata cctatattga gaaggatagt tctgtcaatg acgagattga
    36661 caagcttcgc cactcagcta cctcagccct tttggagcgt aatgatgtta ttgtcgtggc
    36721 ctcagtctct tgtatctatg gtttgggttc gcccaaggaa tacgctgata gtgtcgttag
    36781 tctccgtcct ggtctagaga tttctcgtga taaactcttg aatgacttgg tcgatattca
    36841 gtttgaacgt aatgatattg atttccaacg cggaagattt cgcgttcgtg gggatgtggt
    36901 agagattttc ccagcttccc gagatgaaca tgcctttcga gtagaatttt ttggagacga
    36961 aattgaccgt atccgtgagg ttgaggctct gacaggtcag gtattgggag aggtggatca
    37021 tttggcgatt ttcccagcta ctcactttgt gaccaatgac gaccatatgg aagttgccgt
    37081 tgctaagatt caagcagaat tagaagagca gttagctgtc tttgagaagg aaggtaaatt
    37141 gcttgaagcc cagcgcttga aacaacggac agagtacgat atcgaaatgc tgcgcgagat
    37201 gggctataca aatggggttg aaaattattc tcgccacatg gatggacgta gtgaaggtga
    37261 gcctccttat acgcttctcg actttttccc agatgatttt ttgattatga ttgacgagag
    37321 tcatatgact atgggacaaa taaaaggcat gtacaatgga gaccgttcgc gtaaggaaat
    37381 gctagtaaat tatggtttcc gtttaccgtc tgccttggac aatcgtcctc tccgtcgtga
    37441 ggagtttgaa agccatgtgc atcagattgt ttacgtatca gcgacacctg gtgactatga
    37501 aaatgaacag actgagacgg tgattgagca aatcattcga ccgacagggc ttttggatcc
    37561 tgaggtggaa gtccgtccga ctatgggaca gattgatgat ctcttaggtg aaatcaatgc
    37621 ccgtgttgaa aagaatgaac gaacctttat caccaccttg accaagaaaa tggcagagga
    37681 cttgaccgac tatttcaagg aaatgggcat caaggtcaag tacatgcact cggatatcaa
    37741 gaccttggaa cggacggaga ttatccgtga tctccgcttg ggtgtctttg atgtcttggt
    37801 tggaatcaat ctccttcgtg aagggattga cgttcctgag gttagcttgg ttgccattct
    37861 cgatgctgac aaggaaggtt tccttcgtaa cgagcgtgga ctcatccaga ccattgggcg
    37921 tgccgcccgt aacagtgaag gtcatgtcat catgtatgca gatacggtta cccagtctat
    37981 gcaacgtgct atcgatgaaa ctgcccgccg tcggaaaatt cagatggcct ataatgaaga
    38041 gcatggtatc gtaccacaga caatcaagaa agaaatccgt gacctgattg ccgtgaccaa
    38101 ggcagttgct aaggaagagg acaaggaagt cgacatcaat agcctcaaca aacaagagcg
    38161 caaagaactc gtcaagaaac tagaaaaaca aatgcaagaa gccgtcgaag tgcttgactt
    38221 tgaactggcg gctcagatcc gtgatatgat gttggaagtc aaggccttgg attaggggaa
    38281 tagtatgatt tatttaagaa agttaaagaa agaagatttg atgtctttat gggaaatggc
    38341 ttattcacag cttaatccag tttggaaaca gtatgatgct ccctattatg atgattatca
    38401 gtatttttca aattttaaag aattcgaact acaaaaatca gaatccattt taagcaactc
    38461 aaatcgcctt ggtatttttg ttgatgataa actagttggg actgtttcgc gttattgggt
    38521 atgtaaagaa acaagatgga tggaattggg aattggtatt tatgataaaa aattctggaa
    38581 cactggtatt gggaaagttg ctatgttgca gtggatagat aggacgtttc aggattactt
    38641 ggagttggag catctgggtt tgacaacttg gtcaggaaat cttggtatga tgaaacttgc
    38701 tgaaaaatta agaatgaaaa aagaagctca tattccaaaa gttcgttatt atcaaggtaa
    38761 atattttgat agtattaaat atggtatttt gagagaagac tgggagaaaa taaatgacgg
    38821 ttattatcaa atcaatggaa actcctgaag agatagaagg taaatccctc gttcactggc
    38881 aaacgtggag agaggcttat gatgaccttt tgcctgcaga atttcaggag acaatgacat
    38941 tagaaagatg tcgattcttt agtcaaaagt atccagaaaa tacattgatt gcgataggtg
    39001 atctgaaggt agttggtttt atcagttatg gtaactttcg tgatgagact attcaagctg
    39061 gtgaaattat tgctttatat gttttaaaag actattatgg aaaaggtatc gcacaaaagt
    39121 taatgaaagc agctttgact gctcttaatc atttttctga aattttctta tgggtattga
    39181 aagataacaa gcgcgccatt gctttctatc aaaaaatggg ttttactttt gatggacaag
    39241 aaaaaatact tgaacttgga aagcctataa aggaaaaacg aatggtgttc tattctaaat
    39301 aattttaaaa aagtaaaaga taatatctct ggtagtaagt ctgaaaattt aataaattag
    39361 aaagcgagta aatttatgtt ccgttcccaa ttaacaattt taacaaatat ctgtctgatt
    39421 gaagacctcg aaactcagcg cgtggtgatg cagtatcgcg cccctgaaaa caatcgctgg
    39481 tctggttatg cctttcctgg aggtcatgta gaaaatgatg aggcttttgc ggagtctgtc
    39541 attcgtgaaa tctacgaaga aacagggttg actatccaaa atcctcaact tgtcggcatt
    39601 aaaaattggc cactagatac aggtgggcgc tatattgtca tttgttataa ggcgactgag
    39661 ttctctggta cccttcaatc ttcagaagag ggagaagttt cttgggtgca aaaagaccag
    39721 attccaaact taaatctggc ctatgatatg ctaccattga tggaaatgat ggaagctccc
    39781 gacaagtcag agtttttcta tcctcgccgt acagaagacg attgggaaaa gaaaatcttc
    39841 tagtctttta ctaaataacc tagctgatcc aaggcctcct cgatatagtg gaggtcttgt
    39901 tgtgtttcgg cttcgactag gtggtaatgg atgccatctg tcaattcaga tagaggtcta
    39961 aagtcagaat gctcgacttg ttctagaaaa tgttgcacat ctctgcgaca agacagttta
    40021 agcaaggttt caatttctcc ataaacggga tggtcaatca aggtattctg gacatgccca
    40081 ccattatcga caatagcaag gagttcctga ccgatttctt caacttcatg tttcactttg
    40141 aaaagtttgt gaacataagg attggtatca atttgcttat agatgtagcc acgattggtg
    40201 gataggatag gagcgccatc ggctctcaaa attgcaatgt cctgcacaat gacctgacgt
    40261 gtgacatgaa aatgttccgc caaactttgg ccattaaggg ctttaggagc ttctttcaag
    40321 agttggagca gggcttgttt gcgatctttt gtcatagctt ttccttttaa cggcgttttc
    40381 gaagcacttt atagacagct agtgctaatg tatagtctac catactatgg ataattgtgc
    40441 caaatccaac tagtacaaat agaacataga acatgttttc tacattggta cccgaagttg
    40501 cgtaaaaaag gacacaggct aatacttcag caagggcatg aacaacagcc aaaacaaaat
    40561 tgaaaaccca ggaagatttt ggtttatcta gggtatcggg gaatttttgt aggtaaagag
    40621 ctcctaaagc accaaaagat atatgggaaa aagcccgaaa aacgataacc atgggatagc
    40681 cagccatcaa aaatccaaaa ctagaggcta ggatgacaaa aactgccatc aagggcgaca
    40741 agaacatggc tataaaaata gcgatgtggc tccccaaagt ataggaagca ggtggaatga
    40801 caatcttgaa aggcataaca attggaatca aaatcgcaat agccgttaaa agggctgtca
    40861 ttgtcataaa ttgtgtcttt ttccgtgtat tcacaagaat ctccttttta actgcatata
    40921 cactagtatg gtacaataaa ccagacaata aagcaagaat ttacttgggt ttatagatca
    40981 ttttttagtt aaaagttata gtagattgaa actagaatag t
//
LOCUS       DBEROU010000015        39915 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.015, whole genome shotgun sequence.
ACCESSION   DBEROU010000015 DBEROU010000000
VERSION     DBEROU010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39915
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.015"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..2465)
                     /gene="pbp1b"
                     /locus_tag="ACL8CL_000839"
     CDS             complement(<1..2465)
                     /gene="pbp1b"
                     /locus_tag="ACL8CL_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1B"
                     /protein_id="HGS1330118.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSARPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGVGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVSPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            2607..3863
                     /gene="tyrS"
                     /locus_tag="ACL8CL_000840"
     CDS             2607..3863
                     /gene="tyrS"
                     /locus_tag="ACL8CL_000840"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HGS1330119.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            complement(3941..6004)
                     /locus_tag="ACL8CL_000841"
     CDS             complement(3941..6004)
                     /locus_tag="ACL8CL_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HGS1330120.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTTTDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLVDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(6006..6284)
                     /locus_tag="ACL8CL_000842"
     CDS             complement(6006..6284)
                     /locus_tag="ACL8CL_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6110 family protein"
                     /protein_id="HGS1330121.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            6436..7284
                     /locus_tag="ACL8CL_000843"
     CDS             6436..7284
                     /locus_tag="ACL8CL_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative RNA methyltransferase"
                     /protein_id="HGS1330122.1"
                     /translation="MNTNLKPKLQRFASATAFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTESHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            7907..8479
                     /locus_tag="ACL8CL_000844"
     CDS             7907..8479
                     /locus_tag="ACL8CL_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330123.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLAQLWDKNDAIPNNGRINF"
     gene            complement(9524..11782)
                     /gene="glgP"
                     /locus_tag="ACL8CL_000845"
     CDS             complement(9524..11782)
                     /gene="glgP"
                     /locus_tag="ACL8CL_000845"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HGS1330124.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINSNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGEENIYIFGENSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(11808..13340)
                     /gene="malQ"
                     /locus_tag="ACL8CL_000846"
                     /pseudo
     CDS             complement(11808..13340)
                     /gene="malQ"
                     /locus_tag="ACL8CL_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
     gene            13875..15146
                     /locus_tag="ACL8CL_000847"
     CDS             13875..15146
                     /locus_tag="ACL8CL_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS1330125.1"
                     /translation="MSSKFMKSTAVLGTATLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKVTLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIAGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            15257..16549
                     /locus_tag="ACL8CL_000848"
     CDS             15257..16549
                     /locus_tag="ACL8CL_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HGS1330126.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            16551..17393
                     /locus_tag="ACL8CL_000849"
     CDS             16551..17393
                     /locus_tag="ACL8CL_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HGS1330127.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            17646..18446
                     /locus_tag="ACL8CL_000850"
     CDS             17646..18446
                     /locus_tag="ACL8CL_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HGS1330128.1"
                     /translation="MLPYPFSFFSSIWGFRKPLSKRFELNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTDKVVQDLSEHATIVDGTLTYTGTVSQASSIVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            18456..19442
                     /locus_tag="ACL8CL_000851"
     CDS             18456..19442
                     /locus_tag="ACL8CL_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1330129.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDETKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            19584..19736
                     /locus_tag="ACL8CL_000852"
     CDS             19584..19736
                     /locus_tag="ACL8CL_000852"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330130.1"
                     /translation="MKIKVQTRKLAAGCSKHCFEVVDETDEVSSKHGFEVADRTDEVS
                     NIYTAR"
     gene            complement(19778..20719)
                     /locus_tag="ACL8CL_000853"
     CDS             complement(19778..20719)
                     /locus_tag="ACL8CL_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGS1330131.1"
                     /translation="MKQAKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINALFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(20697..22460)
                     /gene="aspS"
                     /locus_tag="ACL8CL_000854"
     CDS             complement(20697..22460)
                     /gene="aspS"
                     /locus_tag="ACL8CL_000854"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HGS1330132.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEKEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEAPAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETSKTN"
     gene            complement(22611..22814)
                     /locus_tag="ACL8CL_000855"
     CDS             complement(22611..22814)
                     /locus_tag="ACL8CL_000855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330133.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHGFEIVDETDEVS
                     SKHGFEVVDETDEVSNRTTVRRR"
     gene            complement(22856..23086)
                     /locus_tag="ACL8CL_000856"
     CDS             complement(22856..23086)
                     /locus_tag="ACL8CL_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330134.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(23104..23745)
                     /locus_tag="ACL8CL_000857"
     CDS             complement(23104..23745)
                     /locus_tag="ACL8CL_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1330135.1"
                     /translation="MKLLKNLGWFLLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRPLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYISRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(23757..24491)
                     /locus_tag="ACL8CL_000858"
     CDS             complement(23757..24491)
                     /locus_tag="ACL8CL_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HGS1330136.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIILSFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(24494..24691)
                     /locus_tag="ACL8CL_000859"
     CDS             complement(24494..24691)
                     /locus_tag="ACL8CL_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330137.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            complement(24691..24897)
                     /locus_tag="ACL8CL_000860"
     CDS             complement(24691..24897)
                     /locus_tag="ACL8CL_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1330138.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(24907..25374)
                     /locus_tag="ACL8CL_000861"
     CDS             complement(24907..25374)
                     /locus_tag="ACL8CL_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330139.1"
                     /translation="MIDKVVRNLLLTFFFCKMTKIINFLTTIIVKKKKMCYNEFKLRN
                     RKQKGVIMWVLVFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRLLQEMIGK
                     EPIITGVYIGPDNWEVVDVDEEWVKLRSVDKKGKEKFKLQRIEDIQTVEFDGE"
     gene            complement(25374..25610)
                     /locus_tag="ACL8CL_000862"
     CDS             complement(25374..25610)
                     /locus_tag="ACL8CL_000862"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330140.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDRTDEVSSKHGFEVVDETDEVS
                     SKYCFEVVDRTDEVSNHTYGKVKLTWFEEILEEY"
     gene            complement(25607..26896)
                     /gene="hisS"
                     /locus_tag="ACL8CL_000863"
     CDS             complement(25607..26896)
                     /gene="hisS"
                     /locus_tag="ACL8CL_000863"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HGS1330141.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIAMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFAAKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(26974..28221)
                     /locus_tag="ACL8CL_000864"
     CDS             complement(26974..28221)
                     /locus_tag="ACL8CL_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HGS1330142.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWMVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLALSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKFSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNDYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLARTSNFLDLLFTLSIPVGTACFSLVAVWNMQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(28412..29263)
                     /gene="rgg"
                     /locus_tag="ACL8CL_000865"
     CDS             complement(28412..29263)
                     /gene="rgg"
                     /locus_tag="ACL8CL_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HGS1330143.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIIIKSYMKSIDERVELTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKSDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKMEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            29387..29728
                     /locus_tag="ACL8CL_000866"
     CDS             29387..29728
                     /locus_tag="ACL8CL_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGS1330144.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
     gene            29792..29956
                     /locus_tag="ACL8CL_000867"
     CDS             29792..29956
                     /locus_tag="ACL8CL_000867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330145.1"
                     /translation="MKIKGQTRKLAAGCSKHCFEVMDRTDEVSPKHCFEVVDRTDEVS
                     NHTYGKATLT"
     gene            complement(29982..31685)
                     /gene="ilvD"
                     /locus_tag="ACL8CL_000868"
     CDS             complement(29982..31685)
                     /gene="ilvD"
                     /locus_tag="ACL8CL_000868"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HGS1330146.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIIDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(31918..32850)
                     /locus_tag="ACL8CL_000869"
     CDS             complement(31918..32850)
                     /locus_tag="ACL8CL_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HGS1330147.1"
                     /translation="MMRSTKELRYVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     DFGDRYVNVGIMEAEMVGLAAGLSIQGFRPYLHTFGPFSSRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSIIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPEAVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(32847..33704)
                     /locus_tag="ACL8CL_000870"
     CDS             complement(32847..33704)
                     /locus_tag="ACL8CL_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGS1330148.1"
                     /translation="MILSKNREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEERQMLTSVVER
                     LSQELEETE"
     gene            complement(33708..35054)
                     /locus_tag="ACL8CL_000871"
     CDS             complement(33708..35054)
                     /locus_tag="ACL8CL_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS1330149.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACMIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMVIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(35067..35351)
                     /locus_tag="ACL8CL_000872"
     CDS             complement(35067..35351)
                     /locus_tag="ACL8CL_000872"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1330150.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(35355..37385)
                     /locus_tag="ACL8CL_000873"
     CDS             complement(35355..37385)
                     /locus_tag="ACL8CL_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1330151.1"
                     /translation="MFDYRALVILMTLMDHCEVSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLSDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLIDQVVKDYYEKLQLVPIVNQLKVYLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKCYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYNDIKMIIKQED"
     gene            37577..38584
                     /locus_tag="ACL8CL_000874"
     CDS             37577..38584
                     /locus_tag="ACL8CL_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGS1330152.1"
                     /translation="MTEKLINSKPNGVFALILIELTLVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNDNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            38587..38787
                     /locus_tag="ACL8CL_000875"
     CDS             38587..38787
                     /locus_tag="ACL8CL_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS1330153.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            38939..39121
                     /gene="rpmF"
                     /locus_tag="ACL8CL_000876"
     CDS             38939..39121
                     /gene="rpmF"
                     /locus_tag="ACL8CL_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HGS1330154.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKITKAASAE"
     gene            39137..39286
                     /gene="rpmG"
                     /locus_tag="ACL8CL_000877"
     CDS             39137..39286
                     /gene="rpmG"
                     /locus_tag="ACL8CL_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS1330155.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(39778..>39915)
                     /locus_tag="ACL8CL_000878"
     CDS             complement(39778..>39915)
                     /locus_tag="ACL8CL_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330156.1"
                     /translation="ESGSMATGWFTVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWV
                     G"
BASE COUNT        12403 a         8589 c         7090 g        11833 t
ORIGIN      
        1 tatcgtctcg cccttgaaga agaaggtcgt gtagtacttg agttactgct atcgctagaa
       61 ctactacttg aactgctgga gctggatgga gttggtagac tccccacaat actagaccaa
      121 gcattctgat aatccgcatc acttccgcca atagcaaagc gataacttgt cgctggcgct
      181 cctgacttat tagcccaata gctggtaaca gtcgaacctg tgacctctac ttcttttcct
      241 tcaacagaaa ccttccctgg tttttgacct gttgatttca agacttccga tttcactaca
      301 ctaggatcta aagcaaagcg ctcgttcccc caaatgcttg gggaagcttg ctgaatcgca
      361 tttaccagat gagccatgta attagagtta ttagaataac ctgctctacg tgacaatgaa
      421 tgattatcat catgcccaat ccagccacct agggttaatc taggtgtcga aagcatgagc
      481 cacatatttt cgtcttggtt ggttgtacca gtcttcccaa tccaatctgc attagccaga
      541 gtaggattta aagaagtcag gttagacttg aaggttgttg tcacacgaga ggatagaact
      601 tctcgtagca atccctgcat aatcgtcgca gtagcttttg aatagacttg aaccggttta
      661 tcctgatact catacaccac tctaccatct gctgcttcaa tctttgaaat cacatgcttc
      721 tgatgataaa ctccattatt agctaaggtc tgatagccat tggtatgctg ggcaactgtg
      781 acttcaatac caccacccat tggcaagctc tcaataccgt actcaggaat ctcgtaaccc
      841 atcttttcca tataaccctt gacatcaaca cccttttcac ggagcatacg ataggtccag
      901 taagcaggga tattccatga atagttcaga gcttctccca aggtcatcat tcctgttccc
      961 ttgctattag catacataat cggattgcca ttagcaaagt ttgttggata gttagataga
     1021 atcgtttcac ttcccatcaa gccctggtca atagcaatac cgtaggccag caagggcttg
     1081 gtagtagaag ctggcgaacg tttggtatca aaggcatgat tattttgatt ttcttgataa
     1141 ttacgaccac ctacaaagcc tagaatagca cctgtttggt tatccatcaa gacattccct
     1201 acttctacac gacctgttcc atcgtctaaa agatagccat aatcagcaac cgcactttgc
     1261 atggcagaat gaattttctg atctatggta gtagtaatct tataaccacc attttcaatt
     1321 tccttggctg ccaaatctcg ataaaacttc tgagttgcct catttttcaa ctccttagcg
     1381 gagacattgt ctctctgagc tagatagtca tacatacgtt cttgagcttc tgccaaagtt
     1441 gtaaagtata aatagtctcg tgaaattcct gtaaccgtgc ccgatggtaa aaagtcctgt
     1501 ttaaggtcat aatccttgta ctgagaatac tcgtctttgc ttaatgcacc tgtacgatac
     1561 atactgtaaa gaactgcctt agcccgtctt aagccaattt ctaggtcttc atcactcttc
     1621 aactccccag tattttcata aggagagtaa gtaatgggac tctgtggaag tcctgctaaa
     1681 aatgctgctt gaggaacagt caactgactg gcatctacac cgaaaattcc ctcagctgct
     1741 tgccgagccc ctgcaatatt ctgtccctta ttatttcggc caaagggaga cacattgaga
     1801 taggtcgtta aaatctcatc tttattcatg gcgcgttcca aggcaagagc atccacaatc
     1861 tctgccgcct tacgagccaa ggtcggcgca tccccaacca cctgctgttt aattagttgc
     1921 tgggtcaagg ttgaaccccc actagaggaa cccaaaccta caaatttccc caaggtcgca
     1981 cgaatcaccg ccttgggtac tacaccctta tgttctttaa agtgttcatc ttctgtcgca
     2041 atgatagcct tcttcagatt ttccgaaatt tgctcagatg agatagaagt gcgcaacaaa
     2101 tcactctcta tggaagcaat caccgtcccg tccgaatagg taatctctga aatagaagag
     2161 atgtccttga cctgattcac caattcttct gtctgaggca cccgaacctt gtcaaataag
     2221 gccactccgt accccaaagc aatcccaact cccaacattc ctcctagaaa accgagtaca
     2281 aagagtaagt taaataaggc ttttatactc agtaaaatag ctgggaaaat ggctgactta
     2341 tctaaggttt tagatttttt ggtacttgaa cctttcttgc caggtctagc tgatttttta
     2401 tttttttgtt tttgctggaa aaattccagc atttttcgtt ttaattcatt taattgattt
     2461 tgcatggatt tcctcactct atctattata ccacaaaagg gaaattttca ataaaatagc
     2521 cactttcttc cctattctgc taggctattg cccaagtttg tgatacaata ggtagaaaca
     2581 ataattttaa aaaggagaaa agacacatgc acatttttga tgagctaaaa gagcgtggtt
     2641 tgatatttca aacgactgat gaagaagctt tgcgtaaagc cctagaagaa ggtcaagttt
     2701 cttattatac tggctacgat ccaactgctg acagccttca cctaggccac cttgtcgcaa
     2761 tcttgacaag tcgtcgcttg caactagcag gtcacaaacc ttatgcgctc gttggcggtg
     2821 ctacaggtct catcggagat ccgtccttca aagatgctga acgtagtctc caaacaaaag
     2881 acacagtaga tggctgggtc aagtctatcc aaggacaact ttctcgtttt cttgactttg
     2941 aaaatggcga aaacaaggct gtcatggtca acaactacga ctggtttggc agcatcagct
     3001 tcattgactt cctccgtgat attggaaaat acttcacggt caactacatg atgagtaagg
     3061 aatctgttaa aaaacggatc gaaacaggaa tttcttacac tgagttcgct taccaaatca
     3121 tgcaagggta cgacttcttc gtccttaacc aagaccataa tgtcactctt caaatcggtg
     3181 gttctgacca gtggggaaat atgacagctg gtaccgaatt gcttcgtcgt aaggcggaca
     3241 agactggtca cgttatcact gttccactaa tcacagatgc aactggtaag aaatttggta
     3301 aatcagaagg aaatgccgta tggctcaatc ccgaaaagac ttctccatac gaaatgtacc
     3361 aattctggat gaacgtgatg gacgctgacg ctgttcgctt cttgaaaatc tttactttct
     3421 tgtcacttga tgagattgaa gatattcgta aacaatttga agcagcgcca cacgaacgct
     3481 tggctcaaaa agtcttggct cgtgaagttg ttacacttgt tcacggagaa gaagcctaca
     3541 aagaagcact taacatcact gagcaactct ttgcaggaaa catcaaaaac ctttctgtca
     3601 aagagctcaa acaaggactt cgtggtgtgc caaactacca agtacaggca gacgaacata
     3661 acaatatcgt ggaactgctc gtctcatctg gtatagttaa ctcaaaacgc caagcccgtg
     3721 aagacgtcca aaacggagcc atctacgtaa acggcgaccg catccaagac cttgactatg
     3781 tcttgagtga cgctgataag ttagaaaatg aactgactgt tatccgtcgt gggaagaaaa
     3841 aatactttgt attgacttac taaactattc aacatttatc tataaacaaa ggagttaacc
     3901 tcgagaaagg taactccttt ttgctgttaa taactctcat ctatctattt ttaatagaca
     3961 agctacgcaa gacaatgcgc aaggttgtta gattatgtaa gatagagaga tttgaaggac
     4021 tgaaccaatt aaataagcca aagccaatca aactactatt tacgacaacg gtatcttgaa
     4081 tatttttctt gatgagtgtt tgcaaagatg atgataacga atccaactct tggaagaaat
     4141 ccaaacgatt atctaacaat aagatatcac tcatctgctt agaaatatct gcactctcat
     4201 tcatcaccac accgatatct gatagagtta aagccgctga gtcattcaat ccatctccaa
     4261 ccatcaaaat agtgtgacct gctttctgca gtttctctac taactcaaat ttcccatcag
     4321 gtttcaagtc tgtatagacc tgatcaaagg gcaaatcttt gactaattcc tctgtcctaa
     4381 tcaaggtgtc tcctgttgcc agaattaatt ttttcccctg tgccttaagt ttatccaagg
     4441 ctgtttttgc ttcttttctc aaaggagtat gaatgcagaa cattccaatc aattcatttt
     4501 gataagccaa gaataagaga ttgtagtgac tcttgtactc ttcaattaaa gcattttgtt
     4561 ctgaactgat atgaatctgc tcatcctgca tcaagacata attcccaata agaactggtt
     4621 ggccatctat atgagatttg atccccttgc ttgcgatata ttggagtttc ccatgcattt
     4681 cctcatgttc aattccctct atctcagctt gcttgacgat ggcattagca ataggatgat
     4741 aaatgtgttc ctcaagacag gcactgattc tgagaatatc ttcctcacta tagtctccaa
     4801 aaggtaacac cttttcaact ataggataac tagttgtgat tgttcctgtc ttatcaaaca
     4861 agaaagtatc aacttccaga tatttctcca gaacatctcc atccttaatc accatttcac
     4921 ggttcaaccc ttccttgata actgtcaaat aagctacagg agtagagatt ttcaaagcgc
     4981 aggagaaatc gaccaatagg aaagaaatag ccttagaaaa agaacctgtc aataggtaag
     5041 tcagcccagc ccccaagaaa ttatatttga cgactttatc cgccatcttg atgaaatagc
     5101 gttgtttcgt tttcttgttt tcttcagatt tcttcatcaa ctcaatcagc tgtaaaatac
     5161 ggctgttcat ctgattatct gttacacgaa tgcgtaactc tccagtttct aatactgtat
     5221 ttgcacaaac caaatcagac tctctttttt caactggaaa actctctcct gtcaaggaac
     5281 tttcgttgac catacctaaa cctgaaacta cttgcccatc aaacagaatt tcatttcctt
     5341 gagataagat caagacatct cctatttgaa catcggaact ctttatacta acaaccgtat
     5401 cgccctgtac taggaataca tcgctctctt ttgcaagaag actctgttct aaatctgttg
     5461 tagttttttt caaggaccac tgatctaaat gattccccaa atcaagcata aacatgatat
     5521 tgctagctgt cttggattgg ttcataaaca aggacaataa aatagccgaa cagtccaaga
     5581 cttccatcgt tagttcctta cgcgctagtg tttgataggc ttctctaata taacccaaag
     5641 cctgataaca agtccatata tagcgaatag gatacggcac aaaactacga aaaagtacac
     5701 gcttaaccgc tgcacctgaa acaatagaat aagcactctc ttctctacga atgggaagag
     5761 tcatcaactc agaaactttc cctttatcaa ttctttttaa aaaggcttct gcattatcta
     5821 atacagaaaa gccttctttt atgcgtagag taaagtgctg ttgatccatg taaaactgga
     5881 tagactcaat ccccttttca tctctcgcca aggaacgaag atagtcttga atatccaagg
     5941 taagtgaaaa agaagatgat agtcggatat gttggtatcc tctatgtagc actttaaaag
     6001 acatattatt cacctataag gctatctaat tgctcttctt ttttctcttg ctcgtacaaa
     6061 tatttggcat cttgcaagac atcgtctcca tgttgcttca caacagaaac agatgcatct
     6121 agctcgtctt tcaacttgta agccttagcc aaagctttag aataaccttt tttagcttcc
     6181 ttacttgcta agattttcaa accaagggta ccaaatgcga caccacccaa aaataatgaa
     6241 gattttttcg caacttttgc aacggttaat acttctttta acatacttat ttcctcctta
     6301 tcattattat atagcaattt agatataaaa gcaatttcat tctataaatt ggagaattag
     6361 ttttatttct actgaatctc ccatctactt attcctaaag ttgctttcat ttgcctcttt
     6421 tgatacactt aaactatgaa tacaaatctc aagcccaaac ttcagcgttt tgcttctgcg
     6481 actgcctttg cctgtcctat ctgtcaagaa aatctgactc tgttagagac taatttcaag
     6541 tgctgcaacc gtcattcttt tgacttggcg aaatttggct atgtcaatct agcacctcaa
     6601 atcaagcaat ctgctaacta tgacaaagaa aattttcaaa accgtcaaca aatcctagaa
     6661 gccggctttt accaagctat cttagatgct gtatctgact tgcttgcaag ctcaaaaact
     6721 accacaacaa ttttggatat cggttgtggt gaaggattct attctcgcaa actacaagaa
     6781 agtcactctg aaaaaacttt ctatgccttt gacatctcca aagattcagt ccaaatcgct
     6841 gctaaaagtg aacccaactg ggcagtcaat tggttcgttg gcgacttggc acgacttcct
     6901 ataaaagacg ctaacatgga tattctgctt gatatcttct cacctgccaa ctatggagaa
     6961 tttcgtcgcg ttttatccaa agacggtatc ttgataaagg ttatcccaac tgaaagtcac
     7021 ctcaaagaaa tccgtcaaag agtacaggac cagctgacaa acaaggagta ttctaaccaa
     7081 gatatcaagg agcatttcca ggaacacttt accatcctat ctagtcaaac tgcctctctg
     7141 actaagacta tcacagcaga acaactccaa gccctactca gtatgactcc tctcctcttt
     7201 cacgttgacc agagcaagat tgactggagc caactgacag agattaccat cgaagcagag
     7261 attttggttg ggaaagcatt ctaaactaga cttcccaaag gtataaaact taccgaaaat
     7321 cttcaatatt acaaaaacgc ataagaacag gtacacaaac cttaatctta tgcgttttgt
     7381 tatagtttcc atacacatta aggggaaata gttgaaatca gttctcttaa taaccattga
     7441 ataccatata ctacatttac cccaaatatg atataggccg tataaatcaa gtatttcgat
     7501 ttctcccgtc tcaaagaata gatcttgtac aaaatagata aatttaaaat aataaataat
     7561 agagacaaaa tcttcctcca aaatcaatat taaaagtagc cagtcaatca tagttctaat
     7621 ctttattttc gattcgaaat tacaaactta tcaatgaaca ataagtgata taaggccagt
     7681 accagacccc aaacgctacc acgaaaagag aattcttgat gattgaaagt aaacaaatca
     7741 tggttaccaa tatagaggca ggtcaaaata acaaataaaa tccaaaataa atccgtccat
     7801 ttatattttt aaaaatattt ttttaacata atagcatatt tttcttgact ataataacag
     7861 tatataataa aatgtaagca gatacaaata aaaaggagaa aagctcatga acaaattaat
     7921 gaaatttatt tcggtttttt tgacgtcaat tgtgttaatt gtatcagcga ttccaagtgt
     7981 ttcagctgta tacgcttctg aacaagtatc acaaattgaa acaaatatgg aacttcaacc
     8041 tgtcacttct ctaacagaag aacaaatcaa tacacttgca aacgaaatcc aatcttttca
     8101 tccagacgtc tcacaacaat ggatcaaaga agtaattaac cgacaattac aaggcgatta
     8161 tacaatccca cctacatact ctccatttag agcagcttgg caaggtatta cagttaatca
     8221 aatgggtgct ctattagata ctgcaatagc tttagcatta ggaggaacta ctgcaggcct
     8281 tgcaaatcta attaaagtaa aaggaaaaca tgcagcaaaa agtgctattc gttcagcaat
     8341 ttctagatat ctaggtagtt ggtttgtaaa tgatgttgct ttagaatccg ctatgaattt
     8401 attatcaccg gggacttatt tagcacaatt atgggataaa aatgatgcca ttcctaacaa
     8461 cggaaggatt aacttttaat catgagatat acaataaaat taattgggta taacgtattt
     8521 tcgttgttta tattctttat cttatcaatg ttgtttaaag ttccctttag gtggtggatc
     8581 agcattgcaa tgacgacttc gtcgtacatt gtatataatt acttaaagaa aaagagagaa
     8641 aaggaatgat cttataccag atcattcctt ttctgctttc tgtaaatccc tcggagtgtg
     8701 tttggcgctt tgatacgaag tgtcaaagct gtataaacgt tactatttcc aaaaaagtaa
     8761 cagaaaattc ctgctttgta ggactgcttt gggtgagtaa tagacatgtc gactcaccct
     8821 atctcctgat ggcgaaaaga atttagcaat ggtgcaagtt gttctgaact cgagaaaaaa
     8881 catgccgaaa tcgaagaaaa gcatgcagaa cttgaagcga agaagcaaga aatgttgaag
     8941 cgcttggata aatagaattt agaaaacaga aaaaactaaa aagaatgtag ggtttatttc
     9001 cctacattct taaaataact tagtacataa atgacaatgc tgccactgcc gtcaatattg
     9061 caaaaactat tttccaaatt tttttatttt ttccaaaaat cttatcaggt aacaagctta
     9121 ttacaagaaa ggtaataaac aacaccaatt taagtgtgta tatcataaaa acaccttctt
     9181 tcctacaatt atattataat actttatgta agcgaataca agagatatga ggtaaccaac
     9241 tacgaaaaaa tataaggctg aagacagtaa aaaccagcat ttaaaacctt cactgtatct
     9301 aggatttccc tatttttaga caagattgac ccaaatccac agcatttaaa aaagcgaatt
     9361 tcaatcgaaa ctcgcttttt tacaaaatgt attagtataa atttgtatct tatactcaat
     9421 gaaaatcaaa gagcaaacta ggaagctagc cgcaggttgc tcaaagcacc gctttgaggt
     9481 tgcagataaa gctgacgtgg tttgaagaga ttttcgaaga gtattagttc aagtgccaga
     9541 tgtcttcgtt atactgagcg attgtacggt cagatgagaa gaatcctgct ttagaaatgt
     9601 taacgatgac tttatccaac catgcgtcac ggtcttcgta gtcagcaagc atttgctctt
     9661 tgactttgat gtagtcttcc aaatcaagaa gagtcatgaa ccagtctttg ttgatcaatt
     9721 cattgtaaag acgttccaag cgctctttgt ttccagctgc aagaactgca tcactaacga
     9781 tgaagtcaac caatggtttg atagcttcac gagcgtagaa ttcgcttgat ttgtaagctg
     9841 cttttgcgta aaggtcgata acagtttctg aattttcacc gaagatgtag atgttttctt
     9901 ctccaaccaa ctcagcgatt tccacgttag caccgtccat agtaccaagt gtcaaagctc
     9961 cgttcaacat gaatttcatg ttaccagtac ctgaagcttc tttagaagca agtgagattt
    10021 gttctgagat atcacatgct gggataagga aacttgctgc agtaacgttg tagttttcaa
    10081 ccataactac ttgcaagtgt ggagctactg ctggatcgtt agcaataact tctgacatgc
    10141 aaaggattaa atggataatg tcttgagcga ttgtgtaggc tggagctgct ttaccaccaa
    10201 agaagattgt gattggacga gcagggatgt taccagcttt gatgtcaagg tatttgtgga
    10261 tcacgtacaa agcgttcatt tgttggcgtt tgtactcgtg aagacgtttg atttggatat
    10321 caaagataga atttgaattg atttccacac cttggtgttc tttcaagtga cgagccaatt
    10381 tacgtttgtt gtgagccttg atgctttcca atttttcttt gacagctgct ttgtcttcat
    10441 aagacaacag tttttcaagc tcatctgctt catggtgcca accatctcca agaatctcat
    10501 ccaagtagtg agacaatctt gggttagcat gcataagcca acgacggaaa gtgataccgt
    10561 ttgttttgtt gttgaacttt tctgggtaaa ggtcgtagaa ggctttcaac tcagaatttt
    10621 tcaagatttc agtatgaagt gctgcaaccc cgttaacact gtatccgtag tggatatcca
    10681 tgtgagccat gtgaacacgt ccgctctcat cgatgatttg aacagctgga tctttgtact
    10741 ctgccttcac acgacggtcc aattcttcga tgattggtac caagtgagga accacttctt
    10801 gcaagaattc aagaggccat ttttcaagcg cctcagcaag gattgtgtgg ttagtgtagg
    10861 cagtcatgct acgaacaatt gagattgctt cgtcaagctc gataccacgt gcagtcaaaa
    10921 gacgaatcaa ttcaggaatc accattgatg ggtgagtatc gttgatttgg acaactgcgt
    10981 agtcagcaag gtcatgcaag ttgcttcctt tttcgattgc ttcgtcgatg atcaattgcg
    11041 caccgtttga aaccatgaag tattgttgga agatacggag caattcacct tgacggtcac
    11101 tatcatctgg gtaaaggaag agagtcaagt tgcgagcgat atctgtcttg tcaaagttga
    11161 taccatcttt aataatagaa gaatcaactg aatccaagtc aaacaaacgc aagcggtttt
    11221 tagtcgctgt ttcataacca gtaacatcaa tatcgtaaag agttgatgtc aaagtaaagt
    11281 ctgcaaatgg tacttggtag ctacggcttg agcgaaccaa ccagttttgc tctgtcaacc
    11341 atgcatttgg aattgtttct tgttggttgt ttttaagaac ttgttggaaa agaccaaagt
    11401 ggtagttaag accaacaccg tcaccattca aaccaagagt agcaattgag tcgataaagc
    11461 aggcagccaa acgtcccaaa ccaccattac caagagatgg ttccaattca acttcttcaa
    11521 cttcgatcaa gtctttacct gcagctgcaa gttctttttt aacatcgtcg taaagaccaa
    11581 ggttaatcaa gttgtttgac aagagtttac caatcaagaa ctcagctgag atgtagtaaa
    11641 cttttttctt accagtgttg actggttttt ggctgcttgc aagcttgctg tagttaagaa
    11701 gagcaaggta aagctcttca ttgctacatt ctgcaatggt tttattgtaa cgattttgta
    11761 caaattcttg tagtgataac atgtttagtt gtctcctgat tattgtctta tttctttaaa
    11821 tctaccaaat tttcattaat tcggcgataa attgttgtca agtcaagcaa accttcctcg
    11881 acagctggtg tcaattgatc ttcagtcata cgccaagacc agtttccacc aagggtagat
    11941 gggaagttca tacgagctgc ctcatccaat tctagtaaat cttgcatagt tgcaattgcc
    12001 ataaagctaa ctgatgaaaa tactgtacga agcatagcgt gtaccactgt ttcgtattct
    12061 ttacggttcg tgtaacgagc catgtactca cgagtcgcat catcaatctc attacggtac
    12121 caaccaagaa ccgtattgtt atcgtgtgtt cctgtgtaca taactgagtt agcaggtgcc
    12181 aagtgtgggc tatcaatgct ttcgtcttct gggttgaagg caaattgaag aatcttcatt
    12241 cctgggaagc cagtacgttc acgcaattcg atcacttcat ctgtcatgaa gccaaggtct
    12301 tctgcgatga tgtttagctc accaagttct tccttaacgg ctgcaaaaag cttgtagcct
    12361 ggacctttca cccactcacc aggtgctgct gtatcggaac cagcagggat ttcccagtaa
    12421 gattcgaagc cacggaagtg gtcgatacga acgatatcgt agattttgaa gctttcacgc
    12481 aagcgttcaa tccaccattt gtagccgtct ttgtccattg cttcccagtc atagattggg
    12541 ttaccccaaa gctgaccagt tacagaaaac tcatctggtg ggcatcctgc gatacaagta
    12601 gccttaccat tgacatctgt tttgaagaga tgtggatttg cccacatatc acttgaatct
    12661 tccgctacgt agattggcat gtccccaacg atttcgatgt ggttgtcgtt agcgtaagct
    12721 ttcaatttca accattgttg gaagaagaag tattgagtca cacggtggta aaccaacttg
    12781 tctgccaatt gctcacgata gctttcaagt gctgaagctt tacgagcacg agcatctgca
    12841 tctggccatt cagtccaagc aagattgtca aaatactctt tgatagccat atactcagca
    12901 aagagctcaa gccatgattg gttgtcttga gcaaatttct caaaatcttt aacatctccg
    12961 acttcaaaga aacgtttcac cgctttttct aaaagaggac gacgtgcata gtagatttta
    13021 gcatagtcaa cttcagacgc atcgctacca aagtcaactc cttcaaggtc acttgcttcc
    13081 aacaaacctt gctccaccaa gatatctaaa tcgataaaat gagtgtttcc tgcgaaggct
    13141 gagaaagatt ggtaaggaga atccccgtaa ctagttgctc ctaatggaag gatttgccag
    13201 taacgttgtt ttgtacggac caagaaatca acgaagtcgt aagcactttg accaaatgat
    13261 ccgattccgt aagctcctgg aagagaagag atgtgcatca acacaccact ttgacgtttt
    13321 ttcataatag cacctcgtgt gttaaaataa tggaacgttg cgtattttgc agacgcaaac
    13381 gtttgcgtta cttataagta tactcctttt caagcataat tgcaagcgtt tttaaaaaac
    13441 ttttttgatt atttttaagg agactaactc attttagcaa agataactta aaactatcta
    13501 atcctaaagt ttgactctca cgagacagtc tacaaagtac aaaacctcct tagggagctt
    13561 gaatttatat gcaacaaagc acaaacgtct atagaaaata gatagggatt agaaacaggg
    13621 aggtagcccc tcctgatttc cctctttaga cagattccat atatttttat taaaaatact
    13681 gtattcttat ctatttcata cttttcttct attaaaatgc aaaattatgc tatcaaatca
    13741 agcttaaaaa ttttttaaaa tttttacaaa aaatacttgc aaccgttttc tatttgtgct
    13801 atactaagct cataaaggaa aacgtttgcg tttccttatc aataaaactt gctattcttt
    13861 gggaggaata cactatgtca tctaaattta tgaagagcac tgcggtgctt ggaactgcta
    13921 cacttgctag cttgcttttg gtagcttgcg gaagcaaaac tgctgataag cctgctgatt
    13981 ctggttcatc tgaagtcaaa gaactcactg tatatgtaga cgagggatat aagagctata
    14041 ttgaagaggt tgctaaagct tatgaaaaag aagctggagt aaaagtcact cttaaaactg
    14101 gtgatgctct aggaggtctt gataaacttt ctcttgacaa ccaatctggt aatgtccctg
    14161 atgttatgat ggctccatac gaccgtgtag gtagccttgg ttctgacgga caactttcag
    14221 aagtgaaatt gagcgatggt gctaaaacag acgacacaac taaatctctt gtaacagctg
    14281 ctaatggtaa agtttacggt gctcctgccg ttatcgagtc acttgttatg tactacaaca
    14341 aagacttggt gaaagatgct ccaaaaacat ttgctgactt ggaaaacctt gctaaagata
    14401 gcaaatacgc attcgctggt gaagatggta aaactactgc cttcctagct gactggacaa
    14461 acttctacta tacatatgga cttcttgccg gtaacggtgc ttacgtcttt ggccaaaacg
    14521 gtaaagacgc taaagacatc ggtcttgcaa acgacggttc tatcgcaggt atcaactacg
    14581 ctaaatcttg gtacgaaaaa tggcctaaag gtatgcaaga tacagaaggt gctggaaact
    14641 taatccaaac tcaattccaa gaaggtaaaa cagctgctat catcgacgga ccttggaaag
    14701 ctcaagcctt taaagatgct aaagtaaact acggagttgc aactatccca actcttccaa
    14761 atggaaaaga atatgctgca ttcggtggtg gtaaagcttg ggtcattcct caagccgtta
    14821 agaaccttga agcttctcaa aaatttgtag acttccttgt tgcaactgaa caacaaaaag
    14881 tattatatga taagactaac gaaatcccag ctaatactga ggctcgttca tacgctgaag
    14941 gtaaaaacga tgagttgaca acagctgtta tcaaacagtt caagaacact caaccactgc
    15001 caaacatctc tcaaatgtct gcagtttggg atccagcgaa aaatatgctc tttgatgctg
    15061 taagtggtca aaaagatgct aaaacagctg ctaacgatgc tgtaacattg atcaaagaaa
    15121 caatcaaaca aaaatttggt gaataaaaaa tttgttcaag gggggtggaa atcaaatccc
    15181 cctttgaatt tatcaataga gacacaaata atttagcttt cttataaaaa agtagtatcc
    15241 tatgaaagga gttaatatgg aaaagcaaca acctagtaaa gcagccctgc tgtctatcat
    15301 tcctgggtta ggacagattt acaataaaca aaaagccaaa ggttttatct tccttggtgt
    15361 aactatcgta tttgtccttt acttcctagc acttgcaacc cctgaattga gcaacctcat
    15421 cactcttggt gacaaaccag gtcgtgataa ttccctcttt atgctgattc gtggtgcctt
    15481 ccatctaatc tttgtaatcg tttatgtact cttttatttc tcaaatatca aagatgcaca
    15541 tacgattgca aaacgcatta acaatggaat tccagttcca cgcacactca aagacatgat
    15601 caaagggatt tatgaaaatg gcttccctta cctcttgatc attccatctt atgttgccat
    15661 gaccttcgcg attatcttcc cagttatcgt aaccttgatg atcgccttta ccaactacga
    15721 cttccaacac ttgccaccaa acaagttgtt ggactgggtt ggtttgacca actttacaaa
    15781 catttggagc ttgagtacct tccgttctgc ctttggttct gttctttctt ggactatcat
    15841 ttgggctttg gcagcttcta ctttacaaat cgtaattggt atcttcacag ctatcattgc
    15901 caaccaacca tttatcaaag gaaaacgtat ctttggtgtt attttccttc ttccttgggc
    15961 tgtcccagcc ttcatcacta tcttgacatt ctcaaatatg tttaacgata gtgtcggtgc
    16021 tatcaacact caagtattgc caatcttggc taaattcctt cctttccttg atggagctct
    16081 tattccttgg aaaacagacc caacttggac taagattgcc ttgattatga tgcaaggttg
    16141 gctcggattc ccatacatct acgttttgac tttgggtatc ttgcaatcta ttcctaacga
    16201 cctttacgaa gcagcttata ttgacggtgc caacgcttgg caaaaattcc gcaacatcac
    16261 tttcccaatg attttggctg ttgcggcacc tactttgatt agccaataca ccttcaactt
    16321 taacaacttc tctatcatgt acctcttcaa tggtggagga cctggtagtg tcggaggtgg
    16381 agctggttca accgatatct tgatctcatg gatctaccgt ttgacaacag gtacatctcc
    16441 tcaatactca atggcggcag ctgttacctt gattatctct atcattgtca tctcaatctc
    16501 tatgatcgca ttcaagaaac tacacgcatt tgatatggag gacgtctaag atgaataact
    16561 caattaaact caaacgtaga ctgactcaaa gccttactta cctttacctg attggtctat
    16621 caattgtaat tatctatcca ctgttgatta ccattatgtc agcctttaaa gcaggtaacg
    16681 tctcagcctt taaactagat actaatatcg acctcaattt tgataacttt aaaggcctct
    16741 tcactgaaac cttgtacggt acttggtacc tcaacacttt gattatcgcc ttaattacca
    16801 tggctgttca aacaagtatc atcgtacttg ctggttatgc ttacagccgt tacaacttct
    16861 tggctcgtaa acaaagtttg gtcttcttct tgatcatcca aatggtgcca actatggccg
    16921 ctttgacagc cttcttcgtt atggcgctta tgttgaacgc ccttaaccac aactggttcc
    16981 tcatcttcct ctacgttggt ggtggtatcc cgatgaatgc ttggctcatg aaaggctact
    17041 tcgatacagt gccaatgtct ttagacgaat ctgcaaaact agacggtgca ggacacttcc
    17101 gccgcttctg gcaaattgtt ctaccacttg ttcgcccaat ggttgccgta caagctctct
    17161 gggccttcat gggacctttc ggggactaca tcctctctag tttcttgctt cgtgagaaag
    17221 aatactttac tgttgccgta ggtctccaaa ccttcgttaa caatgcgaaa aacttgaaga
    17281 ttgcctactt ctcagcaggt gctatcctca tcgcccttcc aatctgtatt ctcttcttct
    17341 tcctacaaaa gaactttgtt tcaggactta caagtggtgg cgacaaggga taatttatcc
    17401 ccgccaccct ttttcatttt atactcttcg aaaatctctt caaaccacgt cagctttatc
    17461 tccaacctca aagttgtgct ttgagcaacc tgtggctagt ttgcactttg attttcattg
    17521 attattagca attgtcactg taaataatat ccttgtagca agcaattttt ctcctagact
    17581 tgaaataaag cgcatttctc tatataataa tacttatata gaaaacacct tttagaaaga
    17641 tacctatgct tccatatcca ttttcctttt tttcaagtat ttgggggttt cgtaagcccc
    17701 tgtccaaacg tttcgagctc aactggtttc aacttctctt taccagtatc ttccttatca
    17761 gcttgtctat ggtacccatt gctatccaaa acagctccca ggagacctat ccgctagaaa
    17821 cttttatcga taatgtctat gaacctctga cagataaggt tgtccaggat ctctctgaac
    17881 atgctacaat tgtcgatggc acattaactt atactggaac agttagtcaa gcctcttcta
    17941 ttgtgattgg tccaagtcaa atcaaggaat tacctaagga cttgcaactg catttcgata
    18001 caaatgagct agtcatcagc aaggaaagca aggaactgac ccgcatctct taccgagcca
    18061 ttcagactga gagtttcaaa agcaaagaca gcttgaccca agcaatttct aaagactggt
    18121 accaacaaaa tcgtgtctat atcagcctct tcctagttct cggtgcgagc ttcctctttg
    18181 gtttgaattt ctttatcgtc tctctaggag ctagtcttct cctttatatc actaagaaat
    18241 cacgcctctt ttcatttaga acctttaaag agtgctatca ctttatcttg aactgtttag
    18301 gattgccgac tctgattaca cttattttgg gattatttgg ccaaaatatg acaaccctga
    18361 ttactgtaca aaatattctt tttgttctgt atctggtcac tatcttttat aaaacacatt
    18421 tccgtgatcc aaattaccat aaataggaga tttttatgcc cgttacgatt aaagacgtgg
    18481 ccaaggctgc tggtgtttcg ccttcaaccg taacccgtgt tattcaaaat aaatcaacca
    18541 ttagcgacga aacaaaaaaa cgcgttcgca aagctatgaa ggaactcaac taccacccaa
    18601 acctcaacgc tcgtagcttg gtaagcagct atactcaggt tatcggatta gttcttcctg
    18661 atgactcaga cgccttctac cagaatcctt tctttccatc ggttctacgt ggcatctctc
    18721 aagtcgcatc tgaaaaccac tatgccattc agatagcaac agggaaagat gagaaggagc
    18781 gtctcaacgc tatttcacaa atggtctacg gcaagcgtgt agatgggcta atctttctct
    18841 atgcccaaga agaagaccct ctcgtaaaac tcgtcgcaga agaacagttc cccttcctta
    18901 tcttaggtaa atctctatct cctttcatcc cacttgtcga caacgacaat gttcaagctg
    18961 gttttgatgc gactgaatat ttcatcaaaa aaggctgcaa acgcattgcc tttatcggag
    19021 gaagtaaaaa gctcttcgtg accaaagacc gtttaacagg ctatgaacag gcgcttaaac
    19081 attacaaact taccactgac aacaatcgca tctactttgc cgacgagttt ctggaagaaa
    19141 agggctataa atttagcaag cgattattca agcacgatcc acaaattgat gctatcatca
    19201 caaccgatag cctcctagct gaaggtgttt gtaactatat tgccaaacac cagctggatg
    19261 tccctgttct cagctttgac tcggttaatc ccaagctcaa cttggcagcc tatgtcgata
    19321 tcaatagttt agagcttggt cgtgtttccc ttgaaactat tctccagatt attaatgata
    19381 ataaaaacaa taaacaaatt tgttaccgtc aattgatcgc ccacaaaatt atcgaaaaat
    19441 aagagactgg gcaaaaagtc gttaaaagca aaaacgcata ctatcaggta ttgaaaaaac
    19501 ttgatactat gcgttttatt gtgggaagat ttacttcctt ttctactgaa attgagtctt
    19561 ttcccaagat ctttttatac tcaatgaaaa tcaaagtgca aactaggaag ctagccgcag
    19621 gttgctcaaa acactgtttt gaggttgtag atgaaactga cgaagtcagc tcaaaacatg
    19681 gttttgaggt tgcagataga actgacgaag tcagtaacat atatacggca aggtgaagct
    19741 gacgaggttt gaagagattt tcgaagagta ttaatcacta attatctatc tcaacaaatc
    19801 ttcctagaat atgaacattt tccgagacag agacaaagga gcttggatcc acttgtgtca
    19861 taatctgttt aaattcatta aactctgcac gtgtaatgac agtgattaaa actgcctttc
    19921 tctcgtgatt ataggttcct tctgcatcgt ggatcatggt tgctccgcgg tgcaattttt
    19981 tatggatttt ttcaattacc ttctctggat gatttgtcac aatcatggcc tgcatacgct
    20041 tttgcttagt aaagactgcg tctgtcacac gactagagac aaagatggta atcatagaat
    20101 aaagagcgta tttccaacca aaggtcaaac ctgctatcag catgatagtt ccatttacca
    20161 agaaagaaat actaccgaca ttcttacccg ttttcttgcg aatagtcagg ctgacgatat
    20221 ccgtcccacc actggagata ttgtttcgaa gagcaaaacc aatccccaaa cccataacaa
    20281 cacccccaaa aagggcattg ataatgggat cctccgtcaa ggttgccaca gggacaaact
    20341 ggataaagaa ggaactcata gataccgtga taaaggtaaa gacggtgaac ttatggccaa
    20401 tctgatacca agctaagacc atcaaaggga agttaatggc gtagaagctt agcgaaatcg
    20461 gaatatgaaa accaaaccag tgattactca aggcagagat aatctgtgcc agacctgttg
    20521 caccactcga atacacatgc cctggttgga aaaagaaatt aactgctact gctgataaaa
    20581 aaccatagac cagagaggcc gaaatcttct catcatactt ttctcgagag atactttgta
    20641 agacacgtaa aatttttatc tgataagcaa agcggcgcag ataatagcgc caccgcttaa
    20701 ttcgttttgc ttgtttcatc ttcttctact tgtaagctga gttcctctag ttgtttgaga
    20761 gcgactgttg atggagcttg tgtcattggg tcagttgcct tgttgttctt aggaaaggca
    20821 atgacttcac ggatattttc ttctccagca agcaacatga caaaacggtc aagcccgata
    20881 gccaaaccac cgtgtggtgg gaaaccatag tccatggctt caagaaggaa accaaactgg
    20941 tcattggctt cttcagctga gaaaccaaga gccttgaaca tgcgttcttg aaggtctttt
    21001 tggttgatac gaaggctacc accaccaagc tcataaccgt tcaagacgat atcgtaagca
    21061 atggcacgaa ccttagccaa atcaccttct aattcgtgag cagtctcttc ctgtggaagt
    21121 gtgaatggat ggtgggcgct catgtagcgg ccttcttctt cagaccattc aaacatcggc
    21181 cagtcaacca cccaaaggaa gttgaactta tcattatcaa tcaagccaag ctctttagca
    21241 atacgtccac gaagggcacc cagtgttgca ttagccactt caagcgtatc cgccacaaag
    21301 agaaccaagt ccttatcttc aagagcaagc gctgttgtca attcttcttg gataccagtc
    21361 aagaacttgg caactggtcc gtttaattct ccatcaacca ccttgaccca agcaagacct
    21421 ttggcaccat actgtttggc tacttccgtc atcttgtcga tgtctttacg tgaatagttg
    21481 tccgcagctc ctttgaccac aatcgctttt acagcaggtg cttctgaaaa gactttaaag
    21541 tctacacctt tgaccacttc tgtcaagtcc tgaagcaaca tgtcaaaacg agtatctggc
    21601 ttgtcagaac cgtaaagagc catagcatca tcgtatttca tacgagggaa cggtagcgtt
    21661 acttcgatgc cttttgtttc cttcatcacg cgcgcgatca agccttctgt aatatcttgg
    21721 atttctttct cagtaaggaa ggacgtttcc aagtcgacct gagtaaattc aggctggcgg
    21781 tctccacgca agtcctcgtc acggaaacat ttaacgattt ggtagtaacg gtcaaaacca
    21841 gcattcatca agagctgttt cgtgatttgt ggactttgag gaagagcgta aaaatgcccc
    21901 ttattaacac gagacggcac taaataatca cgcgcccctt caggcgttga cttagaaagg
    21961 aatggtgtct ccacgtcgat aaactccaac tcatccaagt agttgcggat agagtgggtc
    22021 accttggcac gaagtttaag attttccaac atttctggac gacgaaggtc aaggtaacgg
    22081 taacgcaaac gtgtatcgtc atttgcctca atgccatcct taatctcaaa tggtgttgtc
    22141 ttagctgtgt taagcacaat aagagctgtc acgtttaact caaccgcacc agttggcaac
    22201 ttatcattgg cttgctcacg cgcagcgacc tgaccagtca cctcaataac aaattcgcta
    22261 cgaaggcttt cagctgttgc cataacctct gcagatactt tttcagggtt gataaccaac
    22321 tgcatgattc cttcacggtc acgaagatcg ataaagatca aaccaccaag gtcacgacga
    22381 cggccaaccc atcctttcaa ggttatttct tgtccgatgt gttcctcacg aacacgacca
    22441 gcatacatac tacgtttcat tatttctctc ctcttttatt ctgttactat tttaccataa
    22501 aagcgcaggc tcttcatgaa aatcatcaga aaagtttgcc agtctttaaa agtcaggtga
    22561 aagccctaaa aattagcgct aatactcttc gaaaatctct tcaaaccacg tcagcgtcgc
    22621 cttaccgtag tacggttact gacttcgtca gtttcatcta caacctcaaa accatgtttt
    22681 gagctgactt cgtcagtttc atctacaatc tcaaaaccat gttttgagct gacttcgtca
    22741 gtttcatcca caacctcaaa acagtgtttt gagcaacctg cggttagctt cctagtttgc
    22801 tctttgattt tcattgagta taatacaaaa atccgatgaa cttcaccgga ctcttttatt
    22861 ttgaattttt gcctgcttta cgcttttcag cgatttcggc tgcctttcga ggcaagacaa
    22921 tttccgttat gtaagccgtc ccaaaacgca gtacacctgc aataggagca aagacaactg
    22981 ctagatagtt atagaagaaa tcgcctttga aggcataagc tagcgctcca atgatgaaaa
    23041 atagaacgac tgcctgaatc actgctaata aaattactcg tttcatgtga cctcctgact
    23101 ctattatagc atgagaatca tcaaaaagcc gactaaatta ttcaaagcgt gaagagaaat
    23161 actgtagacc agaccttttc tgctaatgta agccaaaccc aaactaaaac caaggctaaa
    23221 atagacaaaa aattgttgca catcacctgg aaaatgaatc aaggcaaata gaagactaga
    23281 taccagaaga aaaatcaggg gtcgtttact attgtcctgc ttaggaaaga gatagcgtgc
    23341 taacatccct ctaaaaacaa tctcttccgt caaaggagca aaaataacca cagcaaagaa
    23401 tgagaaaagt ggttgagaca aggtcaagtc tgtcgctatt tgctgattta ctgaaggatc
    23461 atctggcaag aagaattgaa cgaccagaga taagaaccaa accaaggcag gaagccaaat
    23521 aaatcggtta aagccgctct tctcaatata aacaggagct ttctgatacc atttgtaaat
    23581 gccgtacaca tatactccag ccaagaccac atagagtaga gtaacagcat agggtgaagc
    23641 gcctaaagca agcgacgcag tcgcgagccc ctgaataaag ccatagataa agaaaaagga
    23701 tagaagggct agaagaaacc agccaaggtt tttaagtaat ttcatagata actcctttat
    23761 ttgaaataac gttttaccat aggtaactgc atcacattga tataaacatg gatggctcct
    23821 acaagcaaga aagctagtaa ctgaatctct cctgtcaaga aagaaaggat aataagaaaa
    23881 atatataagg ctggtaagac atattggtgt aattggaata aaattcgaaa actctgttcc
    23941 aaattagcct gacgctcccc ttcatcataa gaatttatat agttcaagac atcctttggt
    24001 gtagcgaaaa attccaaatc aaactgacga acaatcgcaa tggttttaaa aagagatttt
    24061 tgagcgacta agaataccac aaagagtaag aaagaaagga aaaatgtttg agggtttgta
    24121 tgcaatataa tcacctcact taatgaaata aaaatagcca atggaatcgc tacacctgta
    24181 atattaaaag caatggttcc aaactcaaga ttccgataca tttgcacata ataggtttca
    24241 ttcagatcgt catccatttc ctcttgatac aaggaatgaa attttctgct tttctttaag
    24301 aaattgaaag tcaaaaacat actaatgaaa cctatcagta aacaaatagc tgatatccat
    24361 ggcatcaagg cttttacatc taaaataatt tcgtgggatt cgacacgtgc cttaaacatc
    24421 cctacaaaca tgcccaagaa cccccccaag acaatagaca tcaaaaataa caatctacgt
    24481 ttctttttca tattcattct cctttttcac ttgctagatt tttggatttc ttttcaatcc
    24541 attcagttac tgggatgaga gcaaagtaga cccaaacaaa ttggtcgctt tgataaggat
    24601 taaaccagct taggtccatc ccaatcagta gaaatacgct gactaataaa gctatgacca
    24661 ctacataata aatcacttta tacttgttca tcactcgtcc tcctccaaac gaaataccga
    24721 ttcgactgtt tcgttgaaaa tttgagatat tttcagggca atgataatgg atggggtgta
    24781 ctcatcccgt tctagtaggc taatggtctg tctggaaacc cctgccagtt tggctaggtc
    24841 ggtttgattg agaccatcgc gagctcgaag ctcttttaga cgatttttta gttgcatgtt
    24901 acacacctac tctccgtcaa attcaacggt ttggatatct tcaatacgtt gcaacttgaa
    24961 tttttctttc ccctttttat caacactacg tagtttgacc cattcctcgt caacatccac
    25021 aacttcccag ttatctggcc caatatacac tcccgttata attggttcct ttccaatcat
    25081 ttcttgcaat aatctcgaca tttctgcgtt tcctttctct tttcgctcaa gttttttgat
    25141 tttattctct agtttcttga tttttttaga attattagaa taaaagaaaa tcataaatag
    25201 tataaatact agtacccaca ttataactcc tttctgcttc ctatttctta acttgaattc
    25261 attgtaacac atctttttct ttttgacaat tatagttgtc aaaaagttta tgatttttgt
    25321 cattttgcaa aagaaaaagg tcaggagtaa gttcctgacc actttatcta tcattaatac
    25381 tcttctaaaa tctcttcaaa ccacgtcagc ttcaccttgc cgtaggtatg gttactgact
    25441 tcgtcagttc tatctacaac ctcaaaacag tattttgagc tgacttcgtc agtttcatct
    25501 acaacctcaa aaccatgttt tgagctgact tcgtcagttc tatccacaac ctcaaaacag
    25561 tgttttgagc aacctgcggc tagcttccta gtttgctctt tgattttcat tgagtataaa
    25621 atcctagttt ttcaaagatt tctgagaagt tttggctgat tgtctcaagt gacacttgca
    25681 cttcttctcg ggtttggttg ttcttgaccg tcacttgtcc gctttcgact tcgctctctc
    25741 ctagggtgat gagggtctta gccgcaaaga catctgctga cttgaactga gctttgagct
    25801 tacggttgag gtaatcacgc tctgctttga aaccttgttg gcgaagagct tggactagtt
    25861 ctagggcttt gacatttgct ccatcaccca agactgcgat atagacatct agggcgtttt
    25921 cgatagggag ggccacgcct tgtttttcaa ggatgagaag caggcgctct acaccaagcc
    25981 caaaaccaaa tccagcagtt tcagggcctc caaagtaagc aaccaaacca tcgtagcgac
    26041 cacccgcaca gactgtcaag tcattgccct caatctctgt gataaactcg aaaatggtgt
    26101 ggttgtagta gtccagacca cgcaccatat tggtatcgat gatgtaatct actccaagat
    26161 tttccaacat ctgacgcaca gcatcaaaat gagcttggct ttcttcatca agaaagtcca
    26221 agatagacgg cgcattctct actgctacct tgtcttcttt ttccttagag tccaagacac
    26281 gaagaggatt ttcctccaag cgacgttggc tatccttaga caaggtctcc ttgagcggtg
    26341 tcaaatagtc aatcaaggct tggcggtagg ctgcacggct ctcaggattt ccaagagtgt
    26401 tgaggtgcaa tttgacacct tgaataccga tttccttcaa aaaatgggct gccatagcga
    26461 ttgtttccac atcggtagct ggattgctag agccaaaaca ctcaacacca atctggtgga
    26521 attggcgcaa gcgccctgcc tgtggacgct cataacggaa cataggtccc atgtagtaga
    26581 acttgcttgg cttttgcact tctggggcga agagtttatt ttccacatag gaacggacaa
    26641 cgggcgcagt tccttctgga cgaagggtaa tatggcggtc acccttgtca tagaaatcgt
    26701 acatttcctt ggttacaata tctgttgtat ctccgacaga gcggctgata acctcgtaat
    26761 gctcgaaaat aggcgtacgc acttctgcgt agttgtagcg tttgaaaatt tcacgggcaa
    26821 agccctcaac gtactgccac ttggcagatt cagcaggtaa aatatcctgc gttccttttg
    26881 gtttttgtaa tttcataggg aatcctcttt aaacttaata gtcttatttt accataaata
    26941 gagggattaa aacagtgaga aaaaaattag gatttagata tcatttttga gattaagaat
    27001 tgttaaaaaa atagctagca aggaaagacc aacaaatagc atccaagtca actgcatgtt
    27061 ccatacggct actagtgaaa aacaagctgt tcccacaggt atggataagg taaacaatag
    27121 atctaaaaaa ttactagtac gagctagaac ctctggagct agatttttca tgagcatggc
    27181 actaatcttt ggttgaactt taccagacac atacagagta aagaagagaa atagcaaacc
    27241 aagcacgact tgattgaata aattagccaa accaactaga ctaagtccta cggtcgccca
    27301 catcatcaat ctaggcaagg actgcttccc aaaataatca ttgcccgtaa ggctactgat
    27361 gatgattgct actaaaacac agaattgatt gataaatagt gcctctgtat aagaaaaatt
    27421 caagagagaa tggctcaaaa agaagatatt ataaattcca cccaaagcgc cacccaagga
    27481 attaataagc aagacagcaa agagcataaa accaaagttt ttctgtccac ttttaagaaa
    27541 aacgagacgt aaatttcggt aaattgttag gaactggtct ttgatagaaa acttctcatt
    27601 ttttaagttt tcaccatcag cagatgacat tgacaggctc aatttgcttt ttcctaaaaa
    27661 gaggatagtg gctgatacta ggaaaaagca ggcattgatt cccgcaacga gagaaaaatt
    27721 gttgaccgat agagctaaga gccagactcc gaaagcttga ccaccaatag ctgaaatata
    27781 ggtgatgaac tgtgaaaaag aataagcctc catcagatca tcttcagcta ctttttcctt
    27841 aataagaggc atacgcaggc cacctgcaaa atcactgatg atatcactaa tgacattgat
    27901 caaacacagg ctagaaaagg caaagagact agcttgctga acaactaggg ctgctagaaa
    27961 aaatagaacc gcctgaaaca aaccgctata gaccatccat ttgaccttgt ccctcgtgta
    28021 atctgcccga atccctgcaa aaactgtaaa gaaggtcgga agaatcatga caatattcgc
    28081 catagcaaca gcaaaagatg cttgtgacaa ggtcgatgca tagacgataa agaccaggtt
    28141 gaaaatcgaa gcaccaaaag cattgaagaa gcgtgataaa gtcaaaattc tataagctgg
    28201 atttctaaac aatagtttca tagataatct ccctccttgt tctaagttct acatcaagaa
    28261 tagctttagt atagcactta tgaatttgcg aaacgattct tttgaatata tgcaacaaac
    28321 aaaatgagca aaaaaacgat agaacttttg taatgacctg agagagggca acagttctat
    28381 cgcttcaaat tttttcttgg tttgcagata ctcaacattc ggtcgtattt ctatagtatt
    28441 cggcagattt attacagcca agcatctcaa aaatacggac agcatcctcc atctttttct
    28501 gaccttcctt gactctacct tgcttgctat caaggagacc ttctgcccac agataaacaa
    28561 ttcggaaata ggtctcattt tccttgtaga aatgctcttc gataacacgt ttaaaataat
    28621 aggcattggt aaattcttca cactcaatac tagctaaaaa gccattcaat agtatagtat
    28681 gaaaaaggtt tcgattgcca gacatttcca ttagaaaatc agatttacgt accatttctc
    28741 gtacatatct agtaaaaaga gaaacagata aaaatggaga actgactgaa aataaattga
    28801 gttcatagat tccccagatc tcggtagaaa acaaataatc atgaaggact tttccttcct
    28861 ctgctgttaa ctctaccctt tcatctatgc tcttcatata agacttgata ataatggcat
    28921 ttagaatatg tttctgtttg ttgtgagaat gggcatgctt ttgatactcc ctgcgatata
    28981 agtcctcaag aggtgctata ttctttggtt ccaagacatc tgtaatttct tttctcaact
    29041 cagaatctgt atcatactgg aaacctctcg ccagaaagag gatctcctcc acactggcag
    29101 atatattttc cagagcaaat agaaactttt ccaccgaaag ctcactctga cctgtttcaa
    29161 aacgggacaa catagacggc gaaaattgtc ctccggttgc ttgtctcagt gagatatttc
    29221 ttgactctcg taattgtcta aagacttttc caatctgctc catagacttc cccttgattc
    29281 cgtattttct tcattttatc atatttttca gaaaattcat caaaaacttg ccaaattgtc
    29341 agaattatga gaaaatagag gatatttatc acgtggaggg actgctatga gagacgatat
    29401 caaaatcaat gaccgtgctt tggccttgca agaccaaatc atcgaaaaac tagagaaagt
    29461 ttttgataca gatgtggaat tggatgttta caatctaggt ctgatttatg aaatcaatct
    29521 ggatgaaacg gggctctgca agattgtcat gaccttcacc gatactgcct gtgattgcgc
    29581 cgaaagcctg cctattgaaa tcgtggcagg tctgaaacaa atcgagggta tcaaagatat
    29641 caaggttgaa gttacctggt cgcctgcttg gaaaatcaca cgaatcagtc gctatggccg
    29701 tattgccctt ggactaccac ctcgttaagc aggccaatca tttttaaaga tgaaaaaagc
    29761 aagccaaaaa tggcttgctt tttgatactc aatgaaaatc aaagggcaaa ctagaaaact
    29821 agccgcaggt tgctcaaaac actgttttga agttatggat agaactgacg aagtcagccc
    29881 aaaacactgt tttgaggttg tggatagaac tgacgaagtc agtaaccata cctacggcaa
    29941 ggcgacgttg acgtgatttg aagagatttt caagtatgag tttatttttt acctgacttg
    30001 tccatattcc agaagtctgt cacggctccg cgtgaagcag atgatacgat atgggcatat
    30061 ttaccgagga caccacggct gtaaagtggt ggcaaggttg tttctgcctt gcgtttttca
    30121 agttcttctt cggatacggc catggaaatt tctttggtat cttggtcaac cgtaacgata
    30181 tcgccggtac ggagataggc aattggtcca ccatcctgag cttcaggagc gatatgtcca
    30241 acaaccagac cataagtacc accagagaaa cgtccgtccg tcaagagggc caccttatct
    30301 ccctgacctt taccaacaat cattgaagaa agtgacagca tctcaggcat accaggacca
    30361 cctttaggtc caacaaaacg aacaacgact acatcgccat caatgatttc atctgtcagg
    30421 acggcctgaa tcgcatcttc ttctgagtca aagaccttag ctggcccaac gtgacgacgc
    30481 actttaacac ctgatacctt ggcaactgca ccgtcaggag caaggttccc gttcaagatg
    30541 ataagcggac catccgcacg ttttggattt tcaagtggca tgataacttt ttggcctgga
    30601 gtcaagtctg caaagtcagc caagttttca gctacagtct taccagtaca tgtgatgcga
    30661 tctccgtgaa ggaaaccatt tgccaacaag tacttcataa ccgcagggac accaccgact
    30721 tcgtagaggt cttggaagac atactgacca gatggtttca agtcggccaa gtgaggcaca
    30781 cgttcttgaa tcgtattgaa gtcctcaagt gacaagtcaa catttgcggc atgggcaatg
    30841 gcgagcaagt gaagagtggc gtttgtagaa ccaccgagag ccatcgttac agtaatagca
    30901 tcttcaaagg cttcacgagt caagatatct gatggtttga gaccaagttc caacatctta
    30961 acaacagcac gtcctgctgc ttcgatatct tctttcttat cagctgattc agctgggtga
    31021 gaggatgacc ctggcaaact catccctaga acttcgatag cagttgccat ggtattagca
    31081 gtatacatac caccacaacc accagggcca gggcaggcat tacattcaag acgtttcacg
    31141 tcctcagctg tcatgtcacc gtggttccat tttccgatac cttcaaagac agaaaccaag
    31201 tcgatatctt taccatcaag atttcccggt gcaatagttc caccataggc gaaaatagct
    31261 gggatatcca tattagcaat agcaatcata gatccaggca tgttcttgtc acagccaccg
    31321 atagcgacga aggcatccac gttgtgacca ctcatagccg cctcgatgga gtccgcgatg
    31381 atgtcacgag atgttagaga gaaacgcata ccaggcgttc ccatagcgat cccgtccgct
    31441 acggtaatgg ttccaaactg tacaggccaa gcgcctgcag atttgacacc ttctttagcc
    31501 agtttcccga aatcatgcaa gtgaatgtta catggtgtat tttccgccca agtcgaaatc
    31561 actcccacaa tcgatgtttc aaagtcctta tctgtcatac cagtcgcacg aagcatagca
    31621 cggttaggtg atttaaccat gctgtcatag atgctactgc ggtgacgttt atctaattca
    31681 gtcatcttat ccctcccatt tcagttttta ctattatagc acaatttaaa ggcaatgaac
    31741 agaataaaat tcttgaattt tcagaaaatt ctatacgaat gtgaaatatt taaaattaaa
    31801 aacaacaaag cggattagtg cactttctga tgaccagaat atgcttttta atccgctttc
    31861 tttaaataac gtaatgtaat ttttacagaa attctttcag ataagtgtat ttaacatcta
    31921 tcttgcatta taaatttcta gaaccttctc ttttatattc gattcactca aaccatactc
    31981 attaagaaga taatccattt tccctacttg accaaatctt tcttgaacac ccatccgatg
    32041 aatttttgtt attccatcat cagagaataa ttcacataaa gcactgccaa ttccacctat
    32101 ctgattgtgg ttttctacag taaatatagt ttttccactt aacattgttt ttatctgttc
    32161 tggtatcggt ttgattctaa ataaatctat cacacctact gaataaccta atttagacag
    32221 ttcatctgca actcgaatac ttggagcaac cattatacca gaagcaacga ttacaagatc
    32281 ttcaccatgc cttaactcaa tgtagccttt agaaaaatct tctccacctt gatatacagc
    32341 ctctggagct tttctaattg ttcgaatata ttttagtcct tttaagtcta atgtctggtt
    32401 caatatttca cgaaattgga tatcatcagt tgcttcaaaa atgatcgatt taggaattaa
    32461 acgtaacaat ccaatttctt caaatggcat atgtgtccca ccattcattt ctgcagttac
    32521 tcctgcatct gatccaatca cagtggcatc caattgtgcg tatccaagag aaataaataa
    32581 ttgatcaaat acccttcgtg aagaaaaagg accaaatgta tgaagataag gtctaaaccc
    32641 ctgaatagac aagcctgctg caaggccgac catttctgct tccataatcc caacattcac
    32701 ataacggtct ccaaagtcct tttcaagatt atgagtagcc atcgaacttg acaaatcggc
    32761 ttctaagact actatatcag aataactttg attagcctct aaaaggaagt ctctatatac
    32821 ataccgtaat tctttcgtac ttctcatcat tctgtttcct ccaattcctg acttaatctt
    32881 tctacaactg aagttaacat ttgtctctcc tctacagtag ggcgaagatg atgattggat
    32941 ttcatttctt ccagctcttg aaccccttga cctttaatag tatctaatac aatgcactta
    33001 ggtgatgaat tatttgactg ttttaattgg acaatccctt cataaatttc tctaatatct
    33061 gaacccttga ccctaatgga ttcaaatcca aatgctgaaa atttttctac gaaatcacct
    33121 ggattacaaa tatcctttgt aaaaccatct aattgttttt tgttatcatc aacaaataca
    33181 attaagttgg ataactgttg atgagaagca aactgtatag cctcccaaca ttgtccctca
    33241 tttaactcac catctccaac aatagcataa gtataaaagg gactctttct tattctctga
    33301 ccatatgcaa gtccagttgc aacactaatt ccttgtccta aagagcccgt tgtcatatct
    33361 atgcctggcg ttagatttct atcaggatga gacggtaatt tggttccatt tgtatttaaa
    33421 gaatataaga attctttgtc aaagaaacca ttcaaataga gtgtactgta tagagctggt
    33481 cctccatgac cttttgataa tatgaaataa tctctatctc gtgctgcaaa tatttctgga
    33541 gtcattggca ttatttcacc ataaagcact gctaaaactt ctacgataga cagacttcct
    33601 ccgtaatgtc cgaatccaag atgattcaat gttctaagag tatttaatcg gatgttggtc
    33661 gcaaattttc ttaactcatc ttctctattt ttacttaaaa tcatccctta ttcctccttt
    33721 gcagatggct ttttaataaa ggatactcca aacataactg ctaaaataag aacaagacca
    33781 atcacaatgc ctgcttgtga gccaaattga tttaacattc ctaaaataat tcctgataga
    33841 ccaaaatctg catctgagaa agttgatcct tggaaaccga gtcctcccaa aactggcatt
    33901 aaaaagactg ggagaaaact gattaaaata ccttgtaaaa atgctccaat agtggctcca
    33961 cgaacaccac cagatgcatt cccaataaca cctgcagtcg ctccacagaa gaaatgaggc
    34021 acaacgcctg gtaagataac aaccgttcct gaagcaatca taattaccat acttactaaa
    34081 ccaccaataa aactagagat aaatccaatt agaactgcat taggtgcata agtataaaca
    34141 atcggacaat ccaaagcagg ttttgaatta ggtacaagac gctctgaaat acctttaaag
    34201 gctggaacaa tttcgcccaa aataaggcga acacctgcta aaataacaaa tacccctgct
    34261 gcaaattgac ctgctaattg taaagcataa actagaccac ttgtaccact actgatttct
    34321 ttttctatat attctgaccc tgcaaagata gctacaataa tgtaaataac tgccatagat
    34381 aaagtgatac taacagtact atcacgtaaa aaagctaaac tctttggaaa tttaatgtcc
    34441 tctgttgatt ttgatttgtc accgataagg ctaccagtaa aaccactcaa ccaatatccc
    34501 aaagaactga aatgacctaa agccaccttg tcatttccag ttaattgaac catatatttt
    34561 tgcacaaatg ctggggaaat actcataata ataccgagtg ctaatcctcc tagtaagatg
    34621 agaggcaagc tagtaaagcc agcaactgat aaaatgaccg caatcataca tgccatatat
    34681 agagtgtggt gccctgttaa aaaaatatat ttaaatcgag taaaacgagc gattaagata
    34741 ttgaacacca ttcctgcaaa cataatcatt gcagtagctg agccatatgt tgttaaagct
    34801 acagctacaa ttgcttcatt attcggcaca acgccagata aatgaaaagc atgctcaaac
    34861 atggtaccaa atggatttaa agaattttgt acaattcctg caccaccaga tacaactaag
    34921 aaaccaacaa aggtcttaat tccaccttta ataatatcag gtaatttctt cttctgaaga
    34981 actaatccta aaattgcaat taaagctact aaaatagctg gtgtactaac aatatccaat
    35041 atgaacttca tcatgacgct agcctcctat ataagtcctt tttcttcaca aagtttagta
    35101 attaattctc gtagttcatc catatcaata atactattta agatacgaac atctccaaga
    35161 tgactagctg aatcagctag atcacgacca acaatccaaa tatcagctgc atttggatct
    35221 gctccaccta aatcataatg ttcaacttct acatccgaaa cattcaaatc actcaataca
    35281 gattcaatat tcatctgtac cataaaactt gaacctaatc ctgaaccaca agctgtacca
    35341 atttttaaca ttatctaatc ctcctgttta attatcattt taatatcatt atagtttttt
    35401 gatgatatta aagtttgaac atgattttta tctcttaaaa ttgttgttaa atgtgacaaa
    35461 gcctttaaat gactctcatt atcaatggct gcaatacaaa tcaacaatct tacctcttgt
    35521 tctggattat ccaataaata aatcggttct tccaaaacta acattgacat tcctatttca
    35581 ttcacacctt catctggccg agcgtgagga attgctactc ccttccctaa attaataaaa
    35641 ggtccaaact cttctacttt ttgaatcatt gcctcagggt agttctcagt tatcttatct
    35701 tgatccaaaa gcggtttagc tgctaaacga atcgcctcct tccatcctaa tttttgcgaa
    35761 ctaacctgat aggtttcttt ggtaatgagt tgttctagca ctggtacaat ttcctttcta
    35821 tcattttttt ggtaaagata attctttaac gccaatctta attccaattc ttgtgtaata
    35881 attccatatc ttttgacaat attcaggatt tgttcaatct caaaatcccc atactctaaa
    35941 ttcggaaaat cttttaacac tagttctact agttgtattg cttgctcttc agtcatcata
    36001 accgaaacta gataatttgg cttttctgtc tccaccttta tggtagaaaa aaccatatca
    36061 tagtcactac tagctttcac ctgtaaatca tcaatctttg aggttcctat aaactcaatt
    36121 tgaggaaata atgctaatag attctctttt aacatcaatg aagaactaac accattagga
    36181 caaatgattg ctgctttata gcatttttga ggcaaagtat ctgctttctt taaataacct
    36241 ccgaaatgga taacaaaata tgctgtttca ctatcaggta tgggattgtc aatagcgtcc
    36301 atcaagggca tcaaagaatc tttgactaat tcaaataaat caggataatg ttctttaaca
    36361 tgcaatacat attcatttga actaggtagg ccgaacttta atctatagta agccggtata
    36421 aggtggcggc gaagattttc tctcaatcct tccctttgtt taaaatgtaa caaagaaata
    36481 tcttccattc tacttataat agcctctgtt aattgattaa agtaaaccgg agcaacatct
    36541 acttcacctt caaagcaact tgataataaa acggtgatat agcgataatc atcctcagaa
    36601 aacaccgtat ctataattcc caaatcaacc actgtatcca ataaaatagt cgttatatct
    36661 tgaataacag gagatactaa tgtctctgaa agacatactc tttcaacatc cctttgatac
    36721 ctacacagaa tgaatactaa accgaaaagg taaactttta attgattaac aataggtact
    36781 agctgtagct tctcataata atctttaact acctgatcaa tcaaatcata agttaatgaa
    36841 tacccccaac tggataaaac ataatccaaa ccccaaatcc ctatggagga ttccagcaac
    36901 tcactaacca tttgaaaagc taaacggtgc ttattccact ctgaaccgtg taaagtataa
    36961 ccttttgctc tactgtaccc tagctccaaa tcattatcta acataatctt tcttaatgat
    37021 tgaatatcag ataaggttgt attcttactt actttcaaaa agtcttggta atgattattc
    37081 gatataaaat ctaatcggca aaaagtgtaa agatagatta aagctaagcg agtcgacttt
    37141 ggtaaaacca attcatccga cttaataata tctgtcaaag actgcttcgt acgatttgat
    37201 aaactatagc gaccttgctt tttatccagc actatccctt tattagctaa ataaggcact
    37261 aaataatcta ttccttcttt gacttccttt ataggtaagc tcaccttaac agataactca
    37321 tataatgata cctcacaatg atccatcaaa gtcatcaaaa taactagtgc tctataatca
    37381 aacatttctc ttcctttcta actacaatta tagtgtaaaa gttataagaa taaaagagta
    37441 tttgagcaca atataatctc accttttaaa agaaattatt gggctgaaag tttttagtga
    37501 ttatttttca actttctatt gacttttaaa aacaaagtga tatcatttag atatcaaata
    37561 atgggaggtc attcttatga ctgaaaaact aatcaattca aaaccaaatg gtgtattcgc
    37621 attgattctc attgaattga cgctcgtact tggtatcttt atatttataa tgggtgttgg
    37681 ttcggaaaac atttttggaa ttattatcgg acctttacta atcgtaattg caggtctagc
    37741 tcatgctggt ttaaaagttg tcaaacctca ggaagctctg gttctgacac tctttggtaa
    37801 ttatacaggt accatcaaag aacctggttt ttactttgtc aatcccttca gcgtagcagt
    37861 caaccctgca aaccacactc gacttggaca aagtggtgat gttagcacaa aatctccttt
    37921 tttaggtgct aaatcatcaa atgacaatga tgtaaatctg gaaattggca agaaacacat
    37981 ttccctcaaa gtcatgacct tgagcaattc tcgtcaaaaa atcaatgatt gcttaggaaa
    38041 ccctgtagaa atcggtatcg cggtaacttg gagagttgtc gataccgcta aggcagtctt
    38101 caacgttgat aactacaaag aatatctttc attgcaatgt gatagcgccc tccgtaatat
    38161 tgtccgcatc tatccttacg atgtgtctcc taatgtggat actacgggtg acgggcaagc
    38221 agatgaaggt agtctccgtg gctctagcga aattgttgct aaccgtattc gtgaagaaat
    38281 ccaaagtcgt gttgaggatg ctggcttgga aatccttgaa gcacgtatca cttacctagc
    38341 ttatgctcca gaaattgctg ccgttatgct tcaacgccaa caagcatctg ccattattga
    38401 tgcacggaag atgattgtag atggtgctgt aggaatggtt gaaatggcac tagaacggct
    38461 caatgaaggg gaattggtag aacttgacga agaacgaaaa gctgccatgg tttcaaatct
    38521 ccttgtcgtt ctttgtggca atcatgatgc acaaccaatt gtcaacacag gaagtcttta
    38581 ctaaagatgg ctgaagctaa aaaaaagcag atcccccttc gactctcaac aaagttatac
    38641 gctgcactcg catcatgggc agaagacgac tttcgttcag tcaatgggca aatcgaatat
    38701 ctcctcacag agtgtgtcaa gcaacggaag aaagacggaa aatacgtatc agaaaccatt
    38761 gacgaaccat ttgagattga tatttaacac cttctcctgt ctgccgtgtc ggataggaga
    38821 attttttcat ctttttttgt caagtaactt tactttacaa aaaaaatgtg ttatcctagt
    38881 atggttgatg aagatcagta gattgaatcg aatataaata cctaaaggag aaatcaaaat
    38941 ggcagtacct gcacgtcgca cttcaaaagc gaagaaaaac aaacgtcgta cacactacaa
    39001 agtaacagct ccatctgtaa actttgacga aactactgga gattactcac gttctcaccg
    39061 tgtatcactt aaaggatact acaaaggacg taaaatcact aaagctgcat cagctgaata
    39121 atagaaggga gataccatgc gcgtaaatat tacacttgaa cacaaagaat ctggtgaacg
    39181 cttgtacctt acttctaaaa acaaacgtaa cactccagac cgccttcaat tgaagaaata
    39241 ctcaccaaaa cttcgcaaac acgttgtgtt tacagaagtt aagtaagcat tcaatacgaa
    39301 tcaatacaga agaaaaacgc ttgatcagcg ttttttcttt ttgttaattt cactatattg
    39361 caagggaaat cattcgaatg actacctttt tcactacctt ttttgaaata agaatacgga
    39421 ttcggacaat taaaagcaag aggaactcaa atgtaagttg gggttccttt tttgtatatt
    39481 tttcaaataa cgcctctaca ctatttgtag caaattcacc aactacagtt gtatcttagt
    39541 taaaataagt tagaatatgt aagtgagtac cagatatacc aagacatcgt caccatctaa
    39601 ggtatattca aaatacaaaa gttgaccaac tagatttctg aatatcctta tatatccatt
    39661 cttaaaattg gtttaaatag cgtagtcttt taaactagtt ttgagaatcc aaaaaatctt
    39721 cctacatatg taagaagatt ttttagttca gaatgattag atttagctaa tggataccta
    39781 tcctacccac tcaccgttag cattgactcg atagccatcg ggtgtagtcg tgtttactaa
    39841 taaatctcct gaattatagg tatagtacca tttaccagaa actgtaaacc aaccagtagc
    39901 cattgaacct gattc
//
LOCUS       DBEROU010000016        39844 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.016, whole genome shotgun sequence.
ACCESSION   DBEROU010000016 DBEROU010000000
VERSION     DBEROU010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39844
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.016"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            9..89
                     /locus_tag="ACL8CL_000879"
     tRNA            9..89
                     /locus_tag="ACL8CL_000879"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43..45,aa:Tyr,seq:gta)
     gene            94..164
                     /locus_tag="ACL8CL_000880"
     tRNA            94..164
                     /locus_tag="ACL8CL_000880"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:126..128,aa:Trp,seq:cca)
     gene            179..251
                     /locus_tag="ACL8CL_000881"
     tRNA            179..251
                     /locus_tag="ACL8CL_000881"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:212..214,aa:His,seq:gtg)
     gene            262..333
                     /locus_tag="ACL8CL_000882"
     tRNA            262..333
                     /locus_tag="ACL8CL_000882"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:294..296,aa:Gln,seq:ttg)
     gene            343..426
                     /locus_tag="ACL8CL_000883"
     tRNA            343..426
                     /locus_tag="ACL8CL_000883"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:377..379,aa:Leu,seq:caa)
     gene            533..1810
                     /locus_tag="ACL8CL_000884"
     CDS             533..1810
                     /locus_tag="ACL8CL_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HGS1330157.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     MKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDETDFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATSEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            1886..3370
                     /gene="thrC"
                     /locus_tag="ACL8CL_000885"
     CDS             1886..3370
                     /gene="thrC"
                     /locus_tag="ACL8CL_000885"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HGS1330158.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPDTYPKVDLNF
                     DKLKDASYQEVAKLVLSAFLDDFTVEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALRERLAA
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKVNFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAKLMNALNSQGQYELTNFDAEILDLFAAEYATEEETAAEIKRVYESDS
                     YIEDPHTAVASAVYRKYQSATGDVTKTVIASTASPYKFPVVAVETVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLEIAPIRHKTTVAAADMKAAVEAYLGL"
     gene            3431..4711
                     /locus_tag="ACL8CL_000886"
     CDS             3431..4711
                     /locus_tag="ACL8CL_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HGS1330159.1"
                     /translation="MFKKNKDILNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIGLLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLLGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            4715..5335
                     /locus_tag="ACL8CL_000887"
     CDS             4715..5335
                     /locus_tag="ACL8CL_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1330160.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQDLLVRVAEDRNLDVEVLSQVRAQSLTEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            5501..6643
                     /locus_tag="ACL8CL_000888"
     CDS             5501..6643
                     /locus_tag="ACL8CL_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGS1330161.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETTRPVEEATPQETTPAEKQETQASPQAASAVEVTTTS
                     SEAKEVASSNGATAAVSTYQPEETKTISTTYEAPAAPDYAGLAVAKSENAGLQPQTAA
                     FKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIAEYAIQNMASRGI
                     SYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG"
     gene            7071..7494
                     /locus_tag="ACL8CL_000889"
                     /pseudo
     CDS             7071..7494
                     /locus_tag="ACL8CL_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890080.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            7505..7864
                     /locus_tag="ACL8CL_000890"
     CDS             7505..7864
                     /locus_tag="ACL8CL_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HGS1330162.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKDLLGVFLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            7971..8614
                     /gene="pcp"
                     /locus_tag="ACL8CL_000891"
                     /pseudo
     CDS             7971..8614
                     /gene="pcp"
                     /locus_tag="ACL8CL_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
     gene            complement(8642..9499)
                     /locus_tag="ACL8CL_000892"
     CDS             complement(8642..9499)
                     /locus_tag="ACL8CL_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HGS1330163.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQAYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVP
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            9629..10771
                     /gene="tgt"
                     /locus_tag="ACL8CL_000893"
     CDS             9629..10771
                     /gene="tgt"
                     /locus_tag="ACL8CL_000893"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HGS1330164.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            10869..12686
                     /locus_tag="ACL8CL_000894"
     CDS             10869..12686
                     /locus_tag="ACL8CL_000894"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HGS1330165.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPSYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTA
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHRMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            12839..13990
                     /gene="nagA"
                     /locus_tag="ACL8CL_000895"
     CDS             12839..13990
                     /gene="nagA"
                     /locus_tag="ACL8CL_000895"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HGS1330166.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14153..15211
                     /locus_tag="ACL8CL_000896"
     CDS             14153..15211
                     /locus_tag="ACL8CL_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HGS1330167.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPKKAIKEIYDLTDGRGVDVAIEAVGIPETFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15362..15727
                     /locus_tag="ACL8CL_000897"
     CDS             15362..15727
                     /locus_tag="ACL8CL_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HGS1330168.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            15803..16744
                     /gene="comGA"
                     /locus_tag="ACL8CL_000898"
     CDS             15803..16744
                     /gene="comGA"
                     /locus_tag="ACL8CL_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus ATPase ComGA"
                     /protein_id="HGS1330169.1"
                     /translation="MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKIASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAN
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            16692..17708
                     /gene="comGB"
                     /locus_tag="ACL8CL_000899"
     CDS             16692..17708
                     /gene="comGB"
                     /locus_tag="ACL8CL_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus assembly protein ComGB"
                     /protein_id="HGS1330170.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLGFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            17710..18036
                     /gene="comGC"
                     /locus_tag="ACL8CL_000900"
     CDS             17710..18036
                     /gene="comGC"
                     /locus_tag="ACL8CL_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="comG operon protein ComGC"
                     /protein_id="HGS1330171.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLTKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLRKLQADGRITEEQVKAYNEYYTKNGGA
                     NRKVND"
     gene            18029..18433
                     /gene="comGD"
                     /locus_tag="ACL8CL_000901"
     CDS             18029..18433
                     /gene="comGD"
                     /locus_tag="ACL8CL_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGD"
                     /protein_id="HGS1330172.1"
                     /translation="MIKAFTMLESLLVLGLVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLTVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            18396..18698
                     /gene="comGE"
                     /locus_tag="ACL8CL_000902"
     CDS             18396..18698
                     /gene="comGE"
                     /locus_tag="ACL8CL_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGE"
                     /protein_id="HGS1330173.1"
                     /translation="MEKLNALRKQKIRAVILLEAVVALAIFASIATLLLGQIQKNRQE
                     EAKILQKEEVLRVAKMALQTGQNQVNINGVEIQVFSSEKGLEVYHGSEQLLAIKEL"
     gene            18661..19122
                     /gene="comGF"
                     /locus_tag="ACL8CL_000903"
     CDS             18661..19122
                     /gene="comGF"
                     /locus_tag="ACL8CL_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGF"
                     /protein_id="HGS1330174.1"
                     /translation="MVQNSCWQSKSYKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVRFHFQFQKGLEREFIYRVEKEES"
     gene            19100..19513
                     /gene="comGG"
                     /locus_tag="ACL8CL_000904"
     CDS             19100..19513
                     /gene="comGG"
                     /locus_tag="ACL8CL_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence type IV pilus minor pilin ComGG"
                     /protein_id="HGS1330175.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQIAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTSKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKVEKKKSEEKPEKKENS"
     gene            19650..20237
                     /locus_tag="ACL8CL_000905"
                     /pseudo
     CDS             19650..20237
                     /locus_tag="ACL8CL_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            20298..21251
                     /locus_tag="ACL8CL_000906"
     CDS             20298..21251
                     /locus_tag="ACL8CL_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1330176.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFKEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGL
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKEWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21302..22492
                     /locus_tag="ACL8CL_000907"
     CDS             21302..22492
                     /locus_tag="ACL8CL_000907"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HGS1330177.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKYIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            22493..22624
                     /locus_tag="ACL8CL_000908"
     CDS             22493..22624
                     /locus_tag="ACL8CL_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330178.1"
                     /translation="MKLKKYSIQGVGKVIFPASFSDEIVQNLAMIGFFEKYGIIVVI"
     gene            22641..23012
                     /gene="rnpA"
                     /locus_tag="ACL8CL_000909"
     CDS             22641..23012
                     /gene="rnpA"
                     /locus_tag="ACL8CL_000909"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HGS1330179.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            22987..23811
                     /locus_tag="ACL8CL_000910"
     CDS             22987..23811
                     /locus_tag="ACL8CL_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HGS1330180.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            23830..24816
                     /gene="jag"
                     /locus_tag="ACL8CL_000911"
     CDS             23830..24816
                     /gene="jag"
                     /locus_tag="ACL8CL_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HGS1330181.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QAESQELEDLGLKVETNFDIEQVATEVMAYVQTIIDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNCYSRTFYITINVNDYVEHRAEVLQTYAQKL
                     AIRVLEEGRSHQTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            24867..25490
                     /locus_tag="ACL8CL_000912"
     CDS             24867..25490
                     /locus_tag="ACL8CL_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HGS1330182.1"
                     /translation="MLNKIRDYLDFAGLQYRNPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQRLRPHFWVYLQRDGQVTEPMMALRLYGTSTDF
                     GISLEVSFIERKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEARKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            25621..27078
                     /locus_tag="ACL8CL_000913"
     CDS             25621..27078
                     /locus_tag="ACL8CL_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HGS1330183.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27101..27382
                     /locus_tag="ACL8CL_000914"
     CDS             27101..27382
                     /locus_tag="ACL8CL_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1330184.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            27459..27944
                     /locus_tag="ACL8CL_000915"
     CDS             27459..27944
                     /locus_tag="ACL8CL_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002267837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1330185.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELDDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            27961..28626
                     /locus_tag="ACL8CL_000916"
     CDS             27961..28626
                     /locus_tag="ACL8CL_000916"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HGS1330186.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDVFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            28630..29493
                     /locus_tag="ACL8CL_000917"
     CDS             28630..29493
                     /locus_tag="ACL8CL_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HGS1330187.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIN
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SRGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            29495..30199
                     /locus_tag="ACL8CL_000918"
     CDS             29495..30199
                     /locus_tag="ACL8CL_000918"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HGS1330188.1"
                     /translation="MNQVINAMRKRVCAANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVAWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPENAVYHSVVLEEVSKMNRFTEQINPRVEPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            30376..32049
                     /locus_tag="ACL8CL_000919"
     CDS             30376..32049
                     /locus_tag="ACL8CL_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1330189.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QNFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32161..33252
                     /gene="ulaG"
                     /locus_tag="ACL8CL_000920"
     CDS             32161..33252
                     /gene="ulaG"
                     /locus_tag="ACL8CL_000920"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HGS1330190.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            33366..35342
                     /gene="tkt"
                     /locus_tag="ACL8CL_000921"
     CDS             33366..35342
                     /gene="tkt"
                     /locus_tag="ACL8CL_000921"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HGS1330191.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAEYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVISMPSTDVFDKQNAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
     gene            35459..35758
                     /gene="yajC"
                     /locus_tag="ACL8CL_000922"
     CDS             35459..35758
                     /gene="yajC"
                     /locus_tag="ACL8CL_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGS1330192.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            35805..36233
                     /locus_tag="ACL8CL_000923"
     CDS             35805..36233
                     /locus_tag="ACL8CL_000923"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HGS1330193.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQEYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            36235..36645
                     /locus_tag="ACL8CL_000924"
     CDS             36235..36645
                     /locus_tag="ACL8CL_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HGS1330194.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            36945..39596
                     /gene="adhE"
                     /locus_tag="ACL8CL_000925"
                     /gene_synonym="adhC"
     CDS             36945..39596
                     /gene="adhE"
                     /locus_tag="ACL8CL_000925"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HGS1330195.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKTRQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARILGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT        12123 a         7121 c         8957 g        11643 t
ORIGIN      
        1 tatattttgg aagggtagcg aagaggctaa acgcggcgga ctgtaaatcc gctccttcgg
       61 gttcgggggt tcgaatccct ccccttccat tttacgggca tagtttaaag gtagaactaa
      121 ggtctccaaa accttcagtg tgggttcaat tcctactgcc cgtgttaata gaattatggc
      181 gggtgtggtg aagtggttaa cacaccagat tgtggctctg gcatgcgtgg gttcgatccc
      241 catcactcgc ctattttata ttggggtata gccaagcggt aaggcaaggg actttgactc
      301 cctcatgcgt tggttcgaat ccagctaccc cagttactat ttgccggcgt ggcggaattg
      361 gcagacgcgc tggactcaaa atccagtgtc cgcaaggacg tgccggttcg accccggccg
      421 ccggtatagt atagtgttag gaacgttgtt attcttcgtt ccttttttat attatttttg
      481 gtataattat agttattcaa attttattta gattaagaaa gtgtagggga gtatgtcttg
      541 ttctatcgat ttattaaaac atcggtattt gaaaaatatt aaagaaaatc ctgaattgtt
      601 tgtcggaatt gagttggagt atcctgttgc aagtttagaa ggggatgcta cagatgttga
      661 agttatgaag gatctatttc attatttagt ttctactttg gatctcaccg tagcaaaggt
      721 agatgatttt ggcaatctga tccagttagt agatccgata agtcaggatg ctattttatt
      781 tgaagtttcc tatacaacga ttgagtttgc atttggtaag gctgaaacga ttcaagaggt
      841 cgaaaatcgt ttcaataatt atatgaatgt aattcagaga aagttagctg aatcaaatca
      901 tgctattgtt ggctgtggta tccatcccaa ctgggataaa aatgagaatt gtccagtggc
      961 ttatccacgc tatcagatgt tgatggatta tttgaatttg agtagaaata ttattaaatc
     1021 agatttacat catttccctg aatatggtac ttttatctgt gggagccagg ttcagctgga
     1081 tatttcaaaa accaactact tacgggtgat taatgctttt actcaaattg aagcggctaa
     1141 ggcttattta tttgcaaact ctgaattttc gggtgcggat tgggatacga aaatttcaag
     1201 ggatattttc tgggaagaat ctatgcatgg tatctatcca gagaatgttg gggtcaatgc
     1261 tagactcctt aatgatgaaa ctgatttttt tgactatcta aatcattctg cgatttttac
     1321 tgcggaacgt gatgggcaga cctattattt ttatcctatt caggctgggg actatttggc
     1381 tacgtccgaa atccaagcat ttgctctgaa tggggatgag gttattattt acccccaaga
     1441 gaaggatttt gaaactcatc gtagttacca gtaccaagat ttaacgactc gaggaacagt
     1501 tgagtttcgt agtgtgtgta cacagccact tgataggact tttgcttctg cagcttttca
     1561 cttgggatta ttggttaatt tagacaagtt agaagcttac ttagaaacag cacctttctt
     1621 taaagtattt ggttatgatt acaagtcttt aaggagacaa ttttctaaga aaaatcttac
     1681 agatgaggaa gaaactacga ttattgaatt ttccaaagac ttactcctac tagctgagga
     1741 gggactagtg gtgagaaata aggaagaaat gacctattta cagcctttga gagaagaatt
     1801 gagcctataa tttctcttat aaagggagaa ttttctgaaa aatcatgata taatggacga
     1861 gactatagat aaaggataga gagtaatgac attagtttat caatcaacgc gtgatgccaa
     1921 caatacagta actgccagcc aagcaatttt gcaaggtttg gcgacggacg gcggtttgtt
     1981 tacaccggat acttatccaa aggtagattt gaactttgac aaattgaaag atgcttctta
     2041 ccaggaagtt gctaagctag ttttgtcagc atttttagat gactttacag ttgaggagtt
     2101 ggactactgt atcaacaatg cctacgatag caaatttgat actccagcta ttgcaccatt
     2161 agtgaaatta gatgggcaat acaatttgga acttttccat ggttcaacga ttgcctttaa
     2221 ggatatggcc ttgtctattt tgccatactt tatgacgact gctgctaaga aacatggttt
     2281 ggagaacaag attgttatct tgacagcgac atctggtgac acggggaaag ctgctatggc
     2341 ggggtttgcg gatgtgcctg gtactgagat tatcgtcttt tatccaaagg gtggtgtcag
     2401 caaggtacaa gagttgcaaa tgaccactca gaatggtgac aatactcatg ttatcgctat
     2461 agatggtaac tttgacgatg cgcaaaccaa cgtgaagcac atgtttaacg acgtggctct
     2521 tcgtgaaaga ttagcggcca acaagttgca attttcatct gctaactcta tgaacattgg
     2581 tcgtctggta ccacagattg tttattatgt ttatgcctac gctcagttgg tcaagactgg
     2641 tgagattgtg gctggcgata aggtcaactt cacagtacca acaggaaact ttggaaatat
     2701 cttggctgcc ttttatgcca aacaaatcgg tctcccagtt ggtaaattga tctgtgcttc
     2761 aaatgacaac aatgttttga cagacttctt caagacacgt gtttacgaca agaaacgtga
     2821 gtttaaggta acaaccagcc catctatgga tatcttggta tcttcaaact tggagcgttt
     2881 gattttccat cttttgggga atgatgcggt taagacggct aaactcatga atgctttgaa
     2941 tagccaaggt caatatgagt tgactaattt tgatgcagag attttggacc tctttgcagc
     3001 tgaatatgcg actgaggaag aaactgcggc agaaatcaag cgtgtttatg agtcagattc
     3061 ttatatcgag gatcctcata cagcggttgc ctcagcagtg tatagaaaat accaatcggc
     3121 cactggagat gtaactaaga cagtgattgc ttcaacagct agtccataca agttcccagt
     3181 agttgcagta gaaactgtaa ctggaaaagc aggtttaaca gactttgaag ccttggctca
     3241 attacatgaa atctcaggcg ttgcagtgcc accagcagtt gatgggcttg aaatagctcc
     3301 aattcgtcac aagacaacag tggcagctgc tgacatgaaa gcagcggttg aggcttattt
     3361 aggactttaa gacagaggga gcaaactcgg ttgggaaacc aactgagttt cttttcatca
     3421 ggaggagaga ttgtttaaga aaaataaaga cattcttaat attgcattgc cagctatggg
     3481 tgaaaacttt ttgcagatgc taatgggaat ggtggacagt tatttggttg ctcatttagg
     3541 attgatagct atttcagggg tttcagtagc tggtaatatt atcaccattt atcaggcgat
     3601 tttcatcgct ctgggagctg ctatttccag tgttatttca aaaagcatag ggcagaaaga
     3661 ccagtcgaag ttggcctatc atgtgactga ggcgttgaag attaccttac tattaagttt
     3721 ccttttagga tttttgtcca tcttcgctgg gaaagagatg ataggacttt tggggacgga
     3781 gagggatgta gctgagagtg gtggactgta tctatctttg gtaggcggat cgattgttct
     3841 cttaggttta atgactagtc tgggagcctt gattcgtgca acgcataatc cacgtctgcc
     3901 tctctatgtt agttttttat ccaatgcctt gaatattctt ttttcaagtc tagctatttt
     3961 tgttctggat atggggatag ctggtgttgc ttgggggaca attctgtctc gtttggttgg
     4021 tcttgtgatt ttgtggtcac aattaaaact gccttatggg aagccaactt ttggtttaga
     4081 taaggaactg ttgaccttgg ctttaccagc agctggagag cgacttatga tgagggctgg
     4141 agatgtagtg atcattgcct tggtcgtttc ttttgggacg gaggcagttg ctgggaatgc
     4201 aatcggagaa gtcttgaccc agtttaacta tatgcctgcc tttggcgtcg ctacggcaac
     4261 ggttatgctg ttggcccgag cagttggaga ggatgattgg aaaagagttg ctagtttgag
     4321 taaacaaacc ttttggcttt ctctgttcct catgttgccc ctgtccttta gtatatatgt
     4381 cttgggtgta ccattaactc atctctatac gactgattct ctagcggtgg aggctagtgt
     4441 tctagtgaca ctgttttcac tacttggaac ccctatgacg acaggaacag tcatctatac
     4501 ggcagtctgg cagggattag gaaatgcacg cctccctttt tatgcgacaa gtataggcat
     4561 gtggtgtatc cgcattggaa caggatatct gctggggatt gtgcttggtt ggggcttgcc
     4621 tggtatttgg gcagggtctc tcttggataa tggttttcgc tggttatttc tacgctatcg
     4681 ttaccagcgc tatatgagct tgaaaggata ggaaatgcaa aaaacagctt ttatttggga
     4741 tttagacggg actttattgg actcttacga agcgatttta tcagggattg aggagacttt
     4801 tgctcagttt tctattcctt atgataagga gaaggtgaga gagtttatct tcaagtattc
     4861 ggtgcaagat ttgcttgtgc gggtggcaga agatagaaat ctggatgttg aggtgctaag
     4921 tcaggtgcgt gcccagagtc tgactgagaa gaatgctcag gtagttttga tgccaggtgc
     4981 gcgtgaggtg ctagcttggg cagacgaatc aggaattcag cagtttatat atactcataa
     5041 ggggaacaac gcttttacca ttctcaagga cttgggggtg gaatcctatt ttacagagat
     5101 tttaaccagt cagagtggct ttgtgcggaa gccaagtcca gaagcggcta cctatctgct
     5161 agataagtat cagttgaatt ctgataatac ttattatata ggggatcgga ctctggatgt
     5221 ggaatttgcc cagaatagtg ggattcaaag tatcaacttt ttagagtcta cttatgaagg
     5281 gaatcacagg attcaagcgt tagcagatat ttcccgtatt tttgagacta agtgataaaa
     5341 agattgtgtc agttttgtga cagagaccta acaaactatt tcaagtaacc tagtttgtta
     5401 caaggaatag acagttctgt taaataggcc cgagagggct ttttttctac attttttgtg
     5461 ttatcataga caggtactca tttgaaagga atttgaaaga atgaagaaaa gaatgttatt
     5521 agcgtcaaca gtagccttgt catttgcccc agtattggca actcaagcag aagaagttct
     5581 ttggactgca cgtagtgttg agcaaatcca aaacgatttg actaaaacgg acaacaaaac
     5641 aagttatacc gtacagtatg gtgatacttt gagcaccatt gcagaagcct tgggtgtaga
     5701 tgtcacagtg cttgcgaatc tgaacaaaat cactaatatg gacttgattt tcccagaaac
     5761 tgttttgaca acgactgtca atgaagcaga agaagtaaca gaagttgaaa tccaaacacc
     5821 tcaagcagac tctagtgaag aagtgacaac tgcgacagca gatttgacca ctaatcaagt
     5881 gaccgttgat gatcaaactg ttcaggttgc agacctttct caaccaattg cagaagctcc
     5941 aaaagaagta gcatcaagtt cagaagttac aaagacagtg attgcttctg aagaagtggc
     6001 accatctacg ggcacttctg tcccagagga gcaaacggcc gaaacaactc gcccagttga
     6061 agaagcaact cctcaggaaa cgactccagc tgagaagcag gaaacacaag caagccctca
     6121 agctgcatca gcagtggaag taactacaac aagttcagaa gcaaaagaag tagcatcatc
     6181 aaatggagct acagcagcag tttctactta tcaaccagaa gagacgaaaa caatttcaac
     6241 aacttacgag gctccagctg cgcccgatta tgctggactg gcagtagcga aatctgaaaa
     6301 tgcaggtctt caaccacaaa cagctgcctt taaagaagaa attgctaact tgtttggcat
     6361 tacatccttt agtggttatc gtccaggaga cagtggagat cacggaaaag gtttggctat
     6421 cgactttatg gtaccagaaa gttcagaatt aggggataag attgcggaat atgctattca
     6481 aaacatggcc agccgtggca ttagttacat catctggaaa caacgtttct atgctccatt
     6541 cgatagcaaa tatgggccag ctaacacttg gaacccaatg ccagaccgtg gtagtgtgac
     6601 agaaaatcac tatgaccacg ttcacgtttc aatgaatgga taaacccgac ttggtaacat
     6661 cattttgacg aatgagatct agctttcgtg ataggaagcg attctcgttc gttttttctt
     6721 tgtcatactc ttcgaaaatc tcttcaaaca acgtcagttt tatctgaaac ctcaaagctg
     6781 tgctttgagc aacctgcgac tagcttccta gtttgctttt tgattttcat tgagtatcaa
     6841 tttgaatgga aaatggaaag ttatcatctt gtaatgagtt aagcaacatt cttgcaatct
     6901 attttatagt agattgaaac tagaatagta cacaactgct tctaaaacat tgttagaaat
     6961 cgatttgact gtcctgatcg atttgtcctg ctcttatttc atttcactat actttatatt
     7021 ataattgact ggtcaaatat tgataaatca ataaaaagag aggggaagaa atgctagaga
     7081 ttcaagattt actgtatcaa ctccgcttgt ctgagcaagt gagtacgcaa ttgtttgaaa
     7141 aagcttggga ttagtttgac acggtatcag attttactgt ttttgctgga gcattctctt
     7201 tgtaaccaaa tggcggttca ggagcgtttg aaaattgatc aggctgcttt gacacggcat
     7261 ttcaagattt tggaaacgga aggtttggtg gagcgtcatc gtaatcctga aaatcagcgg
     7321 gaagtgttgg tagaggctgc gaagtatgcc aaggagcagt tagtggtgaa tccccctctg
     7381 caacatatca aggttaagga agagatggaa agtatcttaa cagagtttga gagaacagaa
     7441 ctcagccgtt tattaaataa attggttttg ggtattgaaa atatagaaat ttaaggagaa
     7501 atagatgtca attattttaa caacgatcgt tgctttggag catttttaca ttttttattt
     7561 ggaaagtatt gccacgcaat cagatgcgac tagtcgtgta tttaatatgg aaaaggaaga
     7621 attggctcat ccgtcagtaa gttcattgtt caaaaatcaa ggaatttata aggatctgct
     7681 aggagtcttt ctcttgtatg gcatttattt ctcacagaat ttagaaattg tgactatttt
     7741 tgtcttattt gtgattggtg ctgcgactta cggctcttta acagcggata aaaaaattat
     7801 tttgaaacaa ggtggatcag ctattttggc cttgattagt attttactct ttaaatacac
     7861 ttgaaggtcg attctaatct cgctaatcct tttcaatcca gaataaggga aatatgttat
     7921 acttgttttt aagaaaaaag tctcattgaa ttggttttga ggagttagaa atgaaagtat
     7981 tagtgacagg ttttgagccc tttggagggg aaaagggcaa tccagctttg gaggccatta
     8041 aaggtttacc agctgaaatc catggtgctg aggtccgttg gctagaggtg ccgacagttc
     8101 ttcacaaatc tgctcaagta ttggaagaag agatgaatcg ttatcaacct gactttgtcc
     8161 tttgtattgg gcaagctggt ggaagaacta gtttgacacc tgaacgagtg gccattaatc
     8221 aagacgatgc acgtacttct gataacgaag ataatcaacc gattgaccgt cccattcgcc
     8281 cagatggtgc ttcggcctac tttagtagtt tgccgattaa agcgatggtt caagctataa
     8341 aaaagaagga ttgccggcct ctgtttccaa tacggcaggg acttttgtct gcagccattt
     8401 gatgtatcag gctctctatt tggtagaaaa gaaatttcca tatgttaagg caggttttat
     8461 gcatattcct tatatgatgg aacaggtggt gaacagaccg cctattccag ctatgagttt
     8521 agtggatatt cggcgaggga tagaagcagc aatcggcgct atgatagaac atggagatca
     8581 ggaactcaag ttggtaggcg gagaaactca ttgatagaaa aaagcttgag gggaaaaacc
     8641 ttcaagcttt tggacgtttt cgagccaata ctgctcggta aaacataatt ttagtgcatt
     8701 ggatataagg taggagtgaa aaactagcaa tgccaaaggt aatccaattg aggaagtacc
     8761 aaggaagaag ctgtaaatct aggacaaagt gctggaactt gtagcccttc ataaaggaac
     8821 ggctagtttt taggattcgt cttggtggga cctgtcctag gtctagacta taacagagaa
     8881 gaaattccac ctgtgaatag gcataatact gtggaatata gaggatattc cctacaatga
     8941 tcaagatgag acttgcaaga aagtagagtc caaagaccat gaggaaacgc tcggtttcaa
     9001 ctgatgagag atctagattt ggaaactcag gatgtagggt gacgaatttt ttggctaaaa
     9061 agctactata aaagaggagg taaatcccaa gtaaattagg gatactccat aaaaagagat
     9121 agaaacgttt gagaagtagg gtcaaaaagg tttgagaaaa gcgctcctca tcaaagagag
     9181 ctaggctgtt ttttacagat ggctccgttt tagaatcttt catgagtgtc agtgttgcat
     9241 agacggaact ggtcaaaaga atagtcccga taaaggagac tagtagagga aagaggtagg
     9301 cttgaagtat ttggccaagt atgctgaaaa atggctgttc taaaacagtc ccgtggatcc
     9361 gagataaggg attaagaaaa ctagataaga tgaccagcat actgggaagg atatagagga
     9421 gaaagagacg gggggtgtca gcctgaaaat gttttgactc ctgacgaatt gtttttaaat
     9481 caatttttgg atagttcatt ctcttattat accatagttc ttatacatag ttcgtgacag
     9541 ttcctacttt ttttgataaa atcatacagt gtgtccttgg gcacactgta tgaactggga
     9601 ctgtctttcc cagcttcgga ggtaaaaaat gtcagattca ccaatcaaat atcgtttgat
     9661 taagaaagaa aaacacacag gagctcgtct aggagaaatc atcactcccc acggtacctt
     9721 tccgacacct atgtttatgc cagttgggac acaagccact gtcaaaactc agtcacctga
     9781 agaattgaag gagatgggtt cgggaattat cctatcaaac acctatcatc tctggcttcg
     9841 ccctggagat gaactcattg cacgcgctgg tggtctccac aagttcatga attgggacca
     9901 gcctatcttg acagatagtg gtggttttca ggtttattct ttagcagata gccgtaatat
     9961 cacagaagaa ggagtaacct ttaaaaatca tctaaatggt tctaagatgt tcctatcccc
    10021 agaaaaagcc atctctattc agaataatct gggttcagac atcatgatgt cctttgatga
    10081 atgtcctcag ttttatcaac cttatgacta cgttaagaaa tcgatcgagc gtaccagccg
    10141 ttgggctgag cgtggtttga aggctcaccg tcgtccacat gaccaaggtt tgtttggaat
    10201 tgtgcaaggt gcaggatttg aagaccttcg ccgccaatca gctcatgatc ttgtcagcat
    10261 ggatttctca ggctactcta tcggtggttt ggcagtggga gaaacccatg aagagatgaa
    10321 tgcggtcttg gactttacaa ctcaactgct acctgaaaat aaacctcgtt atctgatggg
    10381 tgtgggagcg ccagatagct tgatcgatgg ggtcattcgt ggggtggata tgtttgactg
    10441 tgtcttaccg actcgaattg ctcgtaacgg gacttgtatg accagtcaag gacgtttggt
    10501 tgtgaaaaat gcccagtttg ctgaggactt tacgccactg gatcctgagt gtgactgcta
    10561 cacatgtaat aactatacac gcgcttacct tcgtcacctg ctcaaggctg atgaaacctt
    10621 tggtatccgc ttgactagct accacaatct ttacttcttg cttaacctga tgaagcaagt
    10681 gcgacaagcc atcatggatg acaatctctt ggaattccgt gagtattttg tggaaaaata
    10741 tggctataat aagtcaggac gtaatttcta aaatggaatt gatataaaaa aatcctaagt
    10801 tttctcttag gatttttctt ctttttttga tagaataaag tgtacaatga aaggaagaat
    10861 aaactcgtat gcgcattaaa tggttttcct tgattaggat tataggttta cttttggtac
    10921 tcttgtatca cttctttcag acgatctttc ctggaggatt tttcggggta gatgtctttt
    10981 tcacattttc aggtttcctg attacagctc tactcattga agaattttct aagaacaatg
    11041 agattgattt gataggattt tttagaagac gcttttatcg gattgtgcca cctgtggttt
    11101 tgatggtctt ggtgaccatg ccttttactt tcttggttcg ccaagactat gttgctggaa
    11161 ttggtggcca gattgcgggc gtcttaggct ttatgaccaa cttctatgaa ctcctaacag
    11221 gtgggagtta tgaatctcag ttcattcctc atttgtttgt tcataattgg agtttggcag
    11281 ttgaggttca ctactatatt ctttggggat tggcagtttg gttcttatcc aaacaagcta
    11341 aatcaaatgg tcagttgaag gggatggtct ttctcttatc tgctgctgcc ttcttgatca
    11401 gtttcttctc catgtttatt ggtagttttc tagtgacctc ttattcctct gtttatttct
    11461 ccagtttaac tcatgtctat ccattctttt tgggaagtat gttagcaact attgtaggcg
    11521 ttcgtcagac gacttccctc gtcaagcagt tggataaaat ctgggattta cgaaagactt
    11581 tggtagtttt tggaggaggc tttggtttcc tagttctttt gactttcttt gtcaaattca
    11641 cttatctttt tgcctatctt atcggcttct tgcttgccag tcttgcagct cttgccatga
    11701 ttctggcggc gcgtgtctta catgaaaaga cacatcatat acaggagccg aagattatca
    11761 gctttttagc ggatactagc tatgcggttt atcttttcca ttggccttcc tatatcattt
    11821 tctcacagtt gacatcaaat cttcttgctg tattactgac tctgatttgt tcttatggct
    11881 ttgccagtct gtcattctat gtattggaac cttggattgc aggcaagaac acacctattt
    11941 tacaaactct tcgtcccctg ccttatattc acgcaattct tgcagcaggt acaggaatct
    12001 tgaccttcat tgtctgcatg gtgaccttgt tggcaccaca agtgggagcg tttgagacag
    12061 acttgactgt taatggcttg aagcaagctg caacaaatat tggccagacc aaggtgatgg
    12121 cagaacgggc agatgcaaac agtttgggaa ttgctgatgg cactatgtta attggtgact
    12181 cagtggcttt aagggcaaat acagcactac agacagctct tcctggagca cagattaacg
    12241 cgcaggtcag cgtaacaacc aagaccgcaa atgaaatcat gctaaataat agccagaata
    12301 aatttttacc taagacggtg gtcattgcga ctggggtaaa taatcctgag aattacaagg
    12361 atgactggga cagtatcgtg aaaaatcttc ctaagggaca ccgtatgatt ttggtgactc
    12421 cttatgaggg agataagaca aaagagacct atgccatcgt tgagaaggct gctgcctata
    12481 tgagagaatt ggcagagaag acaccttaca ttacgatagc agattggaat caagttgcga
    12541 aagagcatcc agaaatttgg gcaggaacag accaggttca tttcgggagt gagagtagca
    12601 ctatcgaagc aggagcaaaa ttgtatgcag atacgattgc cacagctttg cagacagctc
    12661 aagacaagcc ggttaaatca aaataacttg tattaaaaaa gagaagagtt ggagaaatcc
    12721 agctctttta aaatatctcc aaaaaatagg tctataccat ttacaaataa aaaagaaagg
    12781 tttataatgt aattgacata ataaattgta gaatcaatct tttaaggagg ttaacattat
    12841 gcctaactat attaaagcgg atcagttttt ctacccacac ggagttcgtc gaggtggtta
    12901 cttggaactt gtggacggca agtttgggaa acatgtagag cagattcctg aaggggctga
    12961 ggtgattgac tatacaggtt atagcattgc cccaggtctt gtggatactc atattcatgg
    13021 atatgcaggt gtagatgtga tggacaacaa cattgaaggt acattgcata ctatgagtga
    13081 aggacttctt agtaccggtg ttaccagttt cttacccaca actttaacag ccacttatga
    13141 gcaattgctt gcagtcactg aaaatcttgg aaaccattat aaagaagcaa caggtgctaa
    13201 gattcgtggg atttattatg aaggtccata tttcacagaa acttttaagg gggcacaaaa
    13261 tccaacttat atgagagacc cgggtgttga ggagtttcat tcttggcaaa aagcggcaaa
    13321 tggcttgctt aataaaattg cccttgcacc agaacgtgat ggggtggaag actttgttcg
    13381 tacagttacg ggcgaaggtg tgacggttgc tcttggacat tcaaacgcga cttttgatga
    13441 agccaaaaaa gcagtcgatg ctggagcgag tgtttgggtg catgcctaca atggaatgcg
    13501 tgggttgact caccgtgaat tgggtatggt tggagccatg taccaattgc cacataccta
    13561 tgcagagttg atctgtgatg gtcaccacgt agatccaaag gcttgcgaaa ttcttatcaa
    13621 acaaaaagga acagaaaaca tcgctcttat cacagactgt atgacagctg ggggattgga
    13681 agacggagat tatatgttgg gagaattccc agttgtcgtt gcaaatggaa ctgcacgcct
    13741 caaatcgaca ggtaacttgg caggttctat cctcaaactc aaagatggtt tgaagaatgt
    13801 ggtcgaatgg ggaattgcga atccgcatga agcagtcatg atggccagct tcaacccagc
    13861 taaatccgtt cacatcgatg acgtctgtgg ccaaatccgt gaaggctacg acgctgactt
    13921 catcgtatta gataaagatt tggaattggt agcaacctac ctagatggtg tgaaacgtta
    13981 tcaagcataa gagagaaggc agaagttaga gactagcttc tgctttttta tacagggtac
    14041 tttactggta aataaaaagt taaaaaaatc acaaaaaaag cttgaagaaa caaagaaaaa
    14101 ggcttacaat tgaactataa atattacaaa taaaaaaacg gaggagtgct ttatgaaagc
    14161 ctatacttat gttaaaccag gacttgcttc ttttgttgat gtagacaaac cagttattcg
    14221 caagccaaca gacgctattg tgcgtattgt aaaaaccact atttgtggaa cagacctcca
    14281 tattatcaaa ggggatgttc ctacttgcca aagtggtacc attcttggcc acgaagggat
    14341 tgggattgtt gaagaagttg gggaaggagt ttccaacttc aaaaaaggtg acaaggtctt
    14401 gatttcttgc gtctgtgcct gtggtaaatg ctactactgt aaaaaaggaa tttatgccca
    14461 ctgtgaagac gaagggggct ggattttcgg tcacttgatt gatggtatgc aggctgaata
    14521 tctacgtgtc cctcatgcag ataatactct ttaccatact ccagaagact tgtcagatga
    14581 agctttggtt atgctgtcag acattctgcc tactggatat gaaattggtg tcttaaaagg
    14641 gaaagtagaa cctggttgca gcgtagccat tattggttca ggtccagttg gattggctgc
    14701 tcttttaaca gcccaattct attcaccagc taaattgatt atggtagacc tagacgataa
    14761 ccgcttggaa actgccctat cattcggtgc gactcataag gttaattctt cagaccctaa
    14821 aaaagccatt aaagaaattt atgatttgac agatggtcgt ggtgtggatg tcgctatcga
    14881 agctgtaggg attcctgaaa catttgattt ctgtcaaaag attatcggtg tagacggaac
    14941 ggttgccaac tgtggtgtgc atggtaaacc agttgaattc gatttagata aactttggat
    15001 tcgcaacatc aatgtaacaa ctggtttggt atctacaaat acgactccac aattgttgaa
    15061 agcacttgaa agtcataaga ttgaaccgga aaaattggta actcactatt tcaaactcag
    15121 tgaaattgaa aaagcctacg aagtcttcag taaggcagca gaccaccatg ccattaaggt
    15181 cattatcgaa aacgatatct cagaagccta agtagtaaaa atatttttgt acataagcaa
    15241 atagaaattc agtcatccat cagatggctg gattttttat caaaaaatta agaaatgagc
    15301 atatttcttt ccttgtctgg cggaattggt tataatatac ggtacaaagg aatgaatgaa
    15361 tatgtatcgt gttatagaaa tgtacggaga ttttgaaccg tggtggttct tagaaggttg
    15421 ggaagaagat attgtagcaa gtagaaaatt tgaccagtat tatgatgctc tcaaatacta
    15481 caaaacttgc tggtttagat tggaacaaga atcccctctt tataaaagta gaagcgactt
    15541 gatgaccatt ttttgggacc cggaagacca acgctggtgt gatgaatgtg atgagtattt
    15601 acaacaatac cattctttgg ctcttttgca ggatgagcag gttatcccag acgaaaaact
    15661 acgctcaggc tatgaaaaac aaaccagtca ggaaaggaat cgttcttgcc gtatgaaatt
    15721 aaaatagaga aaagtaactt ttttggagtt gcttttttta tttttctaac tctttgcgaa
    15781 tagtataggt gaggaggtaa gtatggttca agaaattgca caagaaatca ttcgttcagc
    15841 tcggaaaaaa gggacgcagg atatttattt tgtccctaag ttagatgcct atgagcttca
    15901 tatgagggta ggagacgagc gctgtaaaat tggtagctat gattttgaaa agtttgcagc
    15961 cgttatcagt cactttaagt ttgtggcggg tatgaatgtg ggagaaaaaa gacgtagtca
    16021 actgggttcc tgtgattatg cctatgacca gaagatagcg tctctacgtt tatctactgt
    16081 aggcgattat cgggggcatg agagtttggt tatccgtttg ttgcacgatg aggagcagga
    16141 cttgcatttt tggtttcagg atattgaaga attaggcaag cagtacaggc aacggggact
    16201 ctatcttttt gctggtccgg ttgggagtgg taagacgacc ttgatgcatg aattgtccaa
    16261 gtcactcttt aaaggacagc aagttatgtc catcgaagat cctgtcgaaa tcaagcagga
    16321 cgacatgctt cagttgcagt tgaacgaagc aatcggccta acctatgaaa atctaatcaa
    16381 actttccttg cgtcatcgac cagatctctt gattatcgga gaaattcgtg acagcgagac
    16441 ggcgcgtgca gtggtcagag ctagtttgac aggtgcgaca gtcttttcaa ccattcacgc
    16501 caagagtatc cgaggtgttt atgagcgtct gctggagttg ggtgtgagtg aagaagaatt
    16561 ggcagttgtt ctgcaaggag tctgctacca gagattaatc gggggaggag gaatcgttga
    16621 ctttgcaaac agagattatc aagaacacca agcagccaag tggaatgagc aaattgacca
    16681 gcttcttaaa gatggacata tcacaagtct tcaggctgag acggaaaaaa ttagctacag
    16741 ctaagcaaaa aaatatcatc accctattta acaatctctt ttctagcggt tttcatctgg
    16801 tggagactat ctccttttta gataggagtt ccttgttgga caagcagtgt gtgacccaga
    16861 tgcgtacagg cttgtctcag gggaaatcat tctcagaaat gatggaaagt ttgggatgtt
    16921 caagtaccat tgtcactcag ttatctctag ctgaagttca tggcaatctt cacctgagtt
    16981 tgggaaagat agaagaatat ctagacaatc tggccaaggt caagaaaaag ttaattgaag
    17041 tagcgaccta tcctttgatt ttgctgggtt ttcttctctt aattatgctg gggctacgga
    17101 attacctgct cccacaactg gatagtagca atattgccac ccgaattatc ggtaatctgc
    17161 cacaaatttt tctaggcatg gtagggcttg tttccgtgct tgccctttta gcactaactt
    17221 tttataaaag aagttctaag atgagtgtct tttctatctt agcacgcctt ccctttattg
    17281 gaatctttgt gcagacctac ttgacagcct attatgcacg tgaatggggg aatatgattt
    17341 cacagggaat ggagctgacg cagatttttc aaatgatgca ggaacaaggt tcccagctct
    17401 ttaaagaaat cggtcaagat ctggctcaaa ccctgaaaaa tggccgtgaa ttttctcaga
    17461 cgataggaac ctatcctttc tttaggaagg aattgagtct catcatagag tatggggaag
    17521 ttaagtccaa gctgggtagt gagttggaaa tctatgctga aaaaacttgg gaagcctttt
    17581 ttacccgagt caaccgcacc atgaatttgg tgcagccact ggtttttatc tttgtggcac
    17641 tgattatcgt tttactttat gcggcaatgc tcatgcccat gtatcaaaat atggaggtaa
    17701 atttttaaaa tgaaaaaaat gatgacattc ttgaaaaaag ctaaggttaa agcttttaca
    17761 ttggtggaga tgttagtagt cttgctgatt atcagcgtgc ttttcttgct ctttgtacct
    17821 aatctgacca agcaaaaaga agcagtcaat gacaaaggaa aagcagctgt tgttaaggtg
    17881 gtggaaagcc aggcagaact ttatagctta gaaaagaatg aagatgctag cctaagaaag
    17941 ttacaagcag atgggcgcat cacggaagaa caggttaaag cttataatga atactatact
    18001 aaaaatggag gggcaaatcg taaagtcaat gattaaggcc tttaccatgc tggaaagtct
    18061 cttggttttg ggacttgtga gtatccttgc cttgggctta tccggctctg tccagtccac
    18121 ttttgcagca gtagaggagc agattttctt tatggagttt gaagaactct atcgggaaac
    18181 ccaaaaacgc agtgtagcca gtcagcaaaa gactagtcta aatttagatg ggcagacgct
    18241 tagcaatggc agtcaaaagt tgacagttcc taaaggaatt caggcaccat caggccaaag
    18301 tattacattt gaccgagctg ggggcaattc gtccctggct aaggttgaat ttcagaccag
    18361 taaaggagcg attcgctatc aattatatct aggaaatgga aaaattaaac gcattaagga
    18421 aacaaaaaat tagggcagtg attttactgg aagcagtagt cgctctagct atctttgcca
    18481 gcattgcgac cctccttttg ggacaaattc aaaaaaatag gcaagaggaa gcaaaaatct
    18541 tgcaaaagga agaagtcttg agggtagcta agatggcctt gcagacaggt caaaatcagg
    18601 taaacataaa cggagttgag attcaggtgt tttctagcga aaagggattg gaggtctacc
    18661 atggttcaga acagttgttg gcaatcaaag agctataagg tcaaggcttt taccttgtta
    18721 gaatccctgc ttgccctgct tgtcatcagt gggggcttac tcctttttca agctatgagt
    18781 cagctcctca tttcagaagt tcgctaccag caacaaagcg agcaaaagga gtggctcttg
    18841 tttgtggacc aacttgaggt agaattagac cgttcgcagt tcgaaaaagt agaaggcaat
    18901 cgcctataca tgaagcaaga tggcaaggac atcgccatcg gtaagtcaaa gtcagacgat
    18961 tttcgtaaaa ccgatgctag tggacggggg tatcagccta tggtttatgg cctcaaatca
    19021 gctcagatta cagaagacaa tcaactggtt cgctttcatt tccagtttca aaaaggctta
    19081 gaaagggagt tcatctatcg tgtggaaaaa gaagaaagtt aaggcaggtg ttctcctcta
    19141 tgcagtcacc atggcagcta tctttagtct tttgttgcaa ttttatttga accgacaaat
    19201 cgcccactat caagactatg ctttaaataa agaaaaattg gttgcttttg ccatggctaa
    19261 acgaaccaag gataaagccg agcaagaaag tggggaacag gtctttaatc taggtcaggt
    19321 aagctatcaa aacaagaaaa caagcttggt gacgactgtt cgtacgtcta agagccaata
    19381 tgagtttctg tttccttcag tcaaaatcaa agaagagaaa agagataaaa aggaagaggt
    19441 agcgaccgat tcaagcgaaa aagtggagaa gaaaaaatca gaagagaagc ctgaaaagaa
    19501 agagaattcc tagtcaattc aactataatg cgttgaatcc agaatagtcc actgtagttt
    19561 ctagaaaatt gctggaaatg gatgttaagc tccaattcat ttgtttatat cttatttcag
    19621 tttactatac tttgtgctaa actaaagata tgaaacatga ttttaaccac aaagcagaaa
    19681 ctttcgactc ccctaaaaat atcttcctcg caaacttggt atgtcaagca gtcgagaaac
    19741 agattgatat tttatcagac aaagtaattt tagatttcgg tggtggcacg ggtctattag
    19801 ccttgcccct agccaagcag gctaagtcag ttactcttgt agacatttct gagaaaatgt
    19861 tggagtaagc tcgtttgaaa gcggagcagc aagcaatcaa gaatatccag tttttggagc
    19921 aagatttacc gaaaaatccc ttggagaaag agtttgattg ccttgctgtt agtcgggttc
    19981 ttcatcatat gcctgatttg gatgcggatc tctcactgtt tcatcaacat ttgaaggaag
    20041 atgggaaact catcattgct gattttacca agacagaagc taatcatcat ggatttgatt
    20101 tagctgaact ggaaaacaag ctaattgagc atggtttttc atctgtgcat agtcagattc
    20161 tctatagcgc tgaagacctg tttcaaggga atcactcaga attcttttta acagtatccc
    20221 aaaaatcact cgcctagtca gggagtgatt tttctataag gatggaaaaa agaagggaaa
    20281 tttgataaga taggaatatg gattttgaaa aaattgaaca agcttatatc tatttactag
    20341 agaatgtcca agtcatccaa agtgatttgg cgaccaactt ttatgacgcc ttggtggagc
    20401 aaaatagcat ctatctggat ggtgaaactg agctaaacca ggtcaaagac aacaatcagg
    20461 cccttaagcg tttagcacta cgcaaagaag aatggctcaa gacctaccag tttctcttga
    20521 tgaaggctgg gcaaacagaa cccttgcagg ccaatcacca gtttacaccg gatgctattg
    20581 ctttgctttt ggtgtttatt gtggaagagt tgtttaaaga ggaggaaatt actatcctcg
    20641 aaatgggttc tgggatggga attctaggcg ctactttctt gacctcgctt gataaaaagg
    20701 tggattactt gggaatggaa gtggatgatt tgctgattga tctggcagct agcatggcag
    20761 atgtaattgg tttgcaggct ggctttgtcc aaggagatgc cgttcgtcca caaatgctca
    20821 aagaaagcga tgtggtcatc agtgacttgc ctgtcggcta ttatcctgat gacgccgttg
    20881 cgtcgcgcca tcaagttgct tctagccaag aacatactta cgcccatcac ttgctcatgg
    20941 aacaagggct taagtacctc aagtcagacg gatacgctat ttttctagct ccgagtgatt
    21001 tgttgaccag tcctcaaagt gatttgttaa aagaatggct gaaagaagag gcgagtctgg
    21061 ttgctatgat tagtctgcct gaaaatctct ttgctaatgc taaacaatct aagactattt
    21121 ttatcctaca gaagaaaagt gaaatagcag tggagccttt tgtttatcca cttgctagct
    21181 tgcaagatgc aagtgtttta atgaaattta aagaaaattt tcaaaaatgg actcaaggta
    21241 ctgaaatata aaatagattt tgttataata gttgaaaacg cttaaaaagg ggtatcatat
    21301 tatgacaaaa acaattgcaa tcaatgcagg aagttcaagt ttgaaatggc aattatactt
    21361 aatgccagaa gaaaaagtat tggcgaaagg tttgattgaa cgtatcggtt tgaaagattc
    21421 aatttcaact gtaaaatttg acggccgttc tgaacaacaa attttggata ttgaaaatca
    21481 tatacaagcc gttaaaattt tattggatga cttgattcgt ttcgatatta tcaaggctta
    21541 tgacgagatt acaggtgttg gacatcgtgt tgttgctggt ggagaatatt tcaaagaatc
    21601 aacagttgtt gagggagatg ttttagaaaa agttgaagag ttgagtttgt tggctcctct
    21661 acacaacccg gccaatgcag caggtgttcg tgccttcaag gaattgttgc cagacattac
    21721 cagtgtagtt gtttttgata cttccttcca cacaagtatg ccagagaaag cttatcgtta
    21781 ccctctacca acaaaatatt acacagaaaa caaggttcgt aaatacggtg ctcatggtac
    21841 aagtcaccag tttgtagcag gagaagctgc aaaactcttg ggacgtccat tagaagactt
    21901 gaagttaatt acctgtcata ttggtaacgg aggctcaatt acagctgtga aagccggcaa
    21961 atctgtagac acttctatgg ggttcactcc tcttggtggt attatgatgg gaacgcgtac
    22021 aggggatatt gatccagcta tcattcctta tttaatgcaa tatacagagg attttaacac
    22081 accagaagat atcagtcgtg ttcttaaccg tgaatcaggt cttttgggag tttctgctaa
    22141 ttctagcgat atgcgcgata tagaagcagc tgtagcagaa gggaatcacg aggctagctt
    22201 ggcttatgaa atgtatgttg accgtatcca aaaatatatc ggtcagtacc ttgcagtgct
    22261 aaatggagca gatgccattg ttttcacagc aggtgtcggt gaaaatgcag agaatttccg
    22321 tcgtgatgta atctcaggga tttcgtggtt tggttgtgat gttgatgatg aaaagaatgt
    22381 ctttggcgtt acaggagaca tctcaacaga ggcagctaaa atccgtgtct tggttattcc
    22441 aacagatgaa gaattagtca ttgcccgtga cgttgaacgc ttgaaaaaat aagtgaaact
    22501 aaaaaaatat tcaatacaag gagttgggaa agttattttt ccagcttctt tttctgatga
    22561 aattgtccaa aaccttgcta tgattggctt ttttgaaaaa tatggtataa tagtagtaat
    22621 ttaatagatg gagttgagtt ttgaagaaaa actttcgtgt aaaaagagag aaagatttta
    22681 aggcgatttt caaggagggg acaagttttg ctaatcgcaa atttgtggtc taccaattag
    22741 aaaaccagaa aaaccatttt cgagtaggtc tatcagttag caaaaaactg gggaatgccg
    22801 tcactagaaa tcaaattaag cgacggattc ggcatattat ccagaatgca aaagggagtc
    22861 tggtagaaga tgtcgacttt gttgtcattg ctcgaaaagg agtcgaaacc ttgggatacg
    22921 cagagatgga gaaaaatcta ctccatgtat taaaattatc aaagatttac cgggaaggaa
    22981 atgggagtga aaaagaaact aaagttgact agtttgctag gactgtctct gttaatcatg
    23041 acagcctgtg cgactaatgg ggtaactagc gatattacag ccgaatcggc tgatttttgg
    23101 agtaaattgg tttacttctt tgcggaaatc attcgctttt tatcgtttga tattagtatc
    23161 ggagtgggga ttattctctt tacggtcttg attcgtacag tcctcttgcc agtctttcag
    23221 gtgcaaatgg tggcttctag gaaaatgcag gaagctcagc cacgcattaa ggcgcttcga
    23281 gaacaatatc caggtcgaga tatggaaagc agaaccaaac tagagcagga aatgcgtaaa
    23341 gtatttaaag aaatgggtgt cagacagtca gattctcttt ggccgatttt gattcagatg
    23401 ccggttattt tggccttgtt ccaagcccta tcaagagttg actttttaaa gacaggtcat
    23461 ttcttatgga ttaaccttgg tagtgtggat acaacccttg ttcttccgat tttagcagca
    23521 gtattcacct ttttaagtac ttggttgtcc aacaaagctt tgtctgagcg aaatggcgct
    23581 acgactgcga tgatgtatgg gattccagtc ttgattttta tctttgcagt ttatgcgcca
    23641 ggtggagtcg ccctatactg gacagtgtct aatgcttatc aagtcttgca aacctatttc
    23701 ttgaataatc cattcaagat tatcgcagag cgcgaggccg tagtacaggc acaaaaagat
    23761 ttggaaaata gaaaaagaaa agccaagaaa aaggctcaga aaacgaaata aataaggagg
    23821 aatctggtaa tggtagtatt tacaggttca actgttgaag aagcaatcca gaaaggattg
    23881 aaagaattag atattccaag aatgaaggct catatcaaag tcatttctag ggagaaaaaa
    23941 ggctttcttg gtctatttgg taaaaaacca gcccaagtgg atattgaagc gattagtgaa
    24001 acgactgttg tcaaagcaaa tcaacaggta gtaaaaggcg ttccgaaaaa aatcaatgat
    24061 ttgaacgagc ctgtgaagac ggttagtgaa gaaaccgttg accttggtca tgtagttgat
    24121 gctattaaaa aaatagagga agaaggtcaa ggtatttctg atgaagtcaa ggctgaaatc
    24181 ttaaaacatg aaagacatgc cagcactatc ttagaagaaa ctggtcacat tgagatttta
    24241 aatgaacttc aaatcgagga agcgatgagg gaagaagcag gcgctgatga ccttgaaact
    24301 gagcaagacc aagctgaaag tcaagaacta gaagacttgg gcttgaaagt tgaaacgaac
    24361 tttgatattg aacaagtagc tacggaagta atggcttatg ttcaaacgat tattgatgac
    24421 atggatgttg aggctacact ttcaaatgat tataaccgtc gtagcatcaa tttgcaaatt
    24481 gatactaacg aaccaggtcg tattatcggc taccatggta aagtcttgaa ggccttgcaa
    24541 ctgttggctc aaaattatct ttacaactgc tattcaagaa ccttctatat cactattaat
    24601 gtcaatgatt atgttgaaca ccgtgcagaa gtcttgcaga cctatgcgca aaaattggcg
    24661 attcgtgttt tagaagaagg acgtagtcat caaacagatc caatgtcgaa tagtgaacgc
    24721 aagattatcc accgtattat ttcacgtatg gatggcgtga ccagttactc tgaaggtgat
    24781 gagccaaatc gctatgtcgt tgtagataca gaataagtaa aatcagggtt gacctgattt
    24841 tttgctagct agaggaggtt aaactgatgt tgaataagat aagagactat ttagactttg
    24901 ctggtttgca gtaccgtaat cctgataaag cgggagcaga gcgagagaag atgctggcat
    24961 tccgccacaa aggacaagag gcccgaaagg cttttacaga actggccaaa gcctttcaag
    25021 caagccatcc agaatggcaa ctccaacaga ctagtcagtg gatgaatcag gcccagcgtt
    25081 tgagaccaca tttttgggtt tatctacaga gagacggaca agtgacagaa cctatgatgg
    25141 ccttacgttt gtatgggaca tctactgact ttggaatttc tttggaagtc agtttcatcg
    25201 aacgtaagaa ggatgagcaa acactgggca agcaggccaa agttttagac attccaaccg
    25261 ttaaagggat ttattatcta acctactcta atggtcaaag tcaacggtgg gaggcgaatg
    25321 aagaaaagcg tcgtacttta cgcgagaagg tgagaagtca agaagctcga aaagttttag
    25381 tgaaggtaga tgttcctatg acagaaaatt cgtctgaaga agaaatcgta gaaggcttat
    25441 tgaagtctta ttctaaaatt cttccctatt atctagctac gagaaaataa gataatttgt
    25501 aaaacatcat aaatcataca gtccaagagt gaacagtccg ctgtgtaatt cttggtcttt
    25561 ttgtttgcgc tttcgcatta tataataaac ttacaaaaac aattcaaaag gagaacaatt
    25621 atggaagtcg tttcaagtgt tctaaattgg ttttctagca atattttgca gaatcccgca
    25681 tttttcgtag gtttattggt gttgatagga tatgcacttt tgaaaaaacc tgcccatgac
    25741 gttttttcag ggtttgttaa agcaacagta gggtatatgt tgcttaacgt gggtgctggt
    25801 ggtttggtta caacctttcg tccaatctta gcagctctta actacaaatt ccaaattggt
    25861 gcagcggtta tcgaccctta ctttggactt gctgcagcaa acaacaaaat tgcagcagag
    25921 tttccagatt ttgttggaac tgcaactaca gctctattga ttggttttgg aataaatatc
    25981 ttgctcgtag ctcttcgaaa gattacgaag gtaagaaccc tctttattac tggtcacatc
    26041 atggtacaac aagctgcaac agtatctctt atggttctat tcttagtacc acaattgcgc
    26101 aatgcttacg gtacagcagc gattggtatc atctgtggac tttactgggc agttagttca
    26161 aatatgactg ttgaggcaac tcaacgcttg actggtggtg gcggatttgc gattggtcac
    26221 caacagcaat ttgcaatctg gtttgtagat aaagtagcag gacgctttgg taagaaagaa
    26281 gaaagtttag acaatcttaa attacctaag ttcctctcaa tcttccacga tacagttgtt
    26341 gcatctgcta ccttgatgct cgtattcttc ggagccattc ttttaatctt gggtccagac
    26401 attatgtcta ataaagaagt catcacttca ggaactctat tcaatcctgc taaacaagat
    26461 ttctttatgt acattatcca aacagccttt accttctcag tttacttgtt cgttttgatg
    26521 caaggtgtcc gaatgttcgt atctgagttg acaaacgcct tccaaggtat ttcaaacaaa
    26581 ttgttgccag gttcattccc agcggttgac gttgcagctt cttatggatt tggttctcca
    26641 aatgctgtct tgtcaggatt tacctttggt ttgattggtc aattgattac aattgtcttg
    26701 ctcatcgtct ttaaaaatcc gattcttatt attacaggat ttgtaccagt gttctttgac
    26761 aatgcagcca ttgcggtcta cgctgataaa cgcggcggat ggaaagcggc tgttatcctt
    26821 tcctttatat caggtgtcct tcaagttgct ctaggagctc tttgtgtggc ccttctcgat
    26881 ttggcatctt atggtggcta ccatggaaat atcgactttg aattcccatg gcttggattt
    26941 ggatatatct tcaaatacct tggtattgtt ggttatgtac ttgtgtgtct cttcttgctt
    27001 gttattcctc aacttcaatt tgccaaagca aaagataaag agaaatatta caacggtgaa
    27061 gttcaagaag aagcttagta tctagaaaag gagaaataaa atggttaaag tattagcagc
    27121 gtgcggaaat ggaatgggtt catcaatggt tatcaagatg aaggttgaaa atgctctccg
    27181 taagcttaat caaacagatt ttacagtcaa ttcatgcagt gtcggtgaag ctaaaggttt
    27241 agcagtagga tatgacatcg taatcgcttc tcttcatttg attcaagaat tggaagggcg
    27301 aactaatggg aagttaattg ggcttgataa cttgatggat gataaagaaa tcaccgaaaa
    27361 actcagtcaa gcactacagt aaaaggttgg agggggctgg acagaaactg agagttatcg
    27421 tttctgtcct tctccctctt taaataaagg aggcagatat gaatttaaaa caagctttaa
    27481 ttgacaatga ctcgatccga ctaggtttag aggctaacaa ttggaaagaa gcagtcaagg
    27541 tagcagtaga tcccttaatt gaaagtgggg caattttgcc agagtattac gatgctatca
    27601 ttgaatcgac tgaagagtat gggccttact atatcttgat gccaggtatg gctatgcccc
    27661 acgctagacc tgaagctggt gtgcaaagtg atgccttttc attgattacc ttacaaaatc
    27721 ctgttgtatt ttcagatggg aaagaggtat ctgttttgtt ggcactagca gcaacaagct
    27781 caaaaattca cacaagtgta gccattccac aaattattgc cctgtttgaa ttagacgatt
    27841 ctattgcacg tttacaggct tgccagacta aagaagatgt cttagctatg attgaagaat
    27901 ctaaggatag cccttatctc gaaggattgg acttggaaag ttagaaagag gaataaagaa
    27961 atgacaaaaa gaatacctaa tttacaagtt gcattagacc attcagactt gcaaggagcg
    28021 attaaagcag ctgtttctgt tggtcaggaa gtagatatta tcgaagctgg aactgtttgc
    28081 ttgcttcaag ttggaagtga actggctgaa gtcttgcgta gccttttccc agataagatt
    28141 attgtggcag acacaaaatg tgctgatgct ggtggaacag ttgctaaaaa taatgcggtt
    28201 cgtggagcag actggatgac ttgtatctgt tgtgcaacca tccctactat ggaagcagct
    28261 ctaaaggcta tcaagactga acgaggagaa cgaggcgaaa tccagatcga gctttatggc
    28321 gattggactt ttgaacaagc tcagctttgg ctagatgcag gtatctcaca agctatttat
    28381 caccaatctc gtgatgctct tcttgctggt gaaacttggg gtgaaaaaga ccttaataag
    28441 gttaaaaaac tcattgacat gggcttccgt gtatctgtaa caggtggtct agatgtagat
    28501 actctcaaac tctttgaagg tgttgatgtc tttaccttta tcgcaggtcg tggaattaca
    28561 gaggctgcgg atccagcagg agcagcgcgt gccttcaagg atgaaatcaa acgaatttgg
    28621 gggtaaatca tggtacgtcc aattggaatt tatgaaaagg caaccccaac acactttact
    28681 tggctagaac gtttaaattt tgccaaggag ttaggctttg attttgtcga gatgtctatt
    28741 gacgaacgtg acgagcgttt agcaagactt gactggagta aggaagaacg cttggaagtt
    28801 gtcaaagcaa tctatgaaac tggtgttcgt attccttcta tctgtttttc aggccatcgt
    28861 cgctacccat tgggttcaaa agatccagtt ctagaggaaa aatctctaga actcatgaaa
    28921 aaatgtatcg aattagctca agacttggga gttcgtacga ttcaattagc tggttacgat
    28981 gtttactatg aggaaaagtc accccagaca cgccaacgtt ttatcaaaaa tttgagaaaa
    29041 gcctgtgact gggctgaaga agctcaggtg gtacttgcta ttgaaattat ggatgatcct
    29101 ttcatcaata gcatcgaaaa atatttggct atagaaaaag agattgactc tcccttcctc
    29161 tttgtatatc cagatattgg taatgtgtct gcatggcata atgatatcta tagtgagttt
    29221 tatcttggtc atcatgccat cgcagctctc catctcaagg atacttatgc agtgacagaa
    29281 agttcaaggg gccagttccg agatgtacct tttgggcaag gttgtgtcaa atgggaagaa
    29341 gctttcgata ttttaaagga aaccaattat aatggacctt tcctaatcga aatgtggtct
    29401 gaaaattgtg aaacagtaga agaaacacgc gcagccattc aagaggcgca agcttttctc
    29461 tatccactca ttaagaaagc aggtttgatg taagatgaat caagtaatca atgctatgcg
    29521 taaacgagtc tgtgctgcca atcaatcatt gccaaaacat ggacttgtca aatttacctg
    29581 ggggaatgta tctgaagtca atcgcgaact cggtgtcatt gttatcaaac catcaggcgt
    29641 ggattatgac gaattgacac ctgaaaacat ggtagtgact gatctagatg gtaagatcct
    29701 agaaggggat ttaagaccat cttccgacct cccaactcat gtgcaattat ataaggcttg
    29761 gtcagaaatt ggtagtgtgg ttcacaccca ttcgacagaa gctgttgctt gggctcaggc
    29821 aggtcgtgat attcctttct acggaacaac ccatgcagat tatttctacg gttcaatccc
    29881 ttgcgcccgt agtttgacca aggacgaagt agaagtggcc tatgaaaaag atactggcct
    29941 ggttatcgta gaagagtttg aacatcgcgg acttaacccg gttgaagtac caggaattgt
    30001 tgtacgcaat cacggtccat tcacctgggg caaaaatcca gagaatgctg tttatcactc
    30061 tgtcgtacta gaggaagtat caaagatgaa tcgctttaca gaacaaatca atccaagagt
    30121 tgaacctgct ccccagtaca tactagaaaa acactaccaa cgtaaacatg gaccaaatgc
    30181 ttattatggt caaaagtaag aacgatgaag gaggagaaaa agataaattt agctcctctt
    30241 tttacatttg atttttattg agagtaaagt tggagttgaa gtaattttga aagatttttt
    30301 agaaatagcg cttgatatat atggtaaaat aaaaagaatt gctgtgatat caatagattt
    30361 gggggatttt ttaatatggt actggataag gcaagttgtg atttgcttca atatttgatg
    30421 gatcaagaaa cgtccaaaac gattatggcg atttcgaaag atttgaaaga gtcaagaagg
    30481 aaaatttatt atcacattga caaaatcaat gctgctctgg ttgacgaggc gcttcacatc
    30541 attagtattc cacgaattgg tattcactta acggaagagc agagagatgc ttgttgtaaa
    30601 ctattatcgg aagtagattc gtacgattat atcatgagtg cgcatgaacg tatgatgata
    30661 atgttactat ggataggtat ttctaaagaa cgtattacga ttgaaaaatt gatagagtta
    30721 acagaggtat ctaggaatac tgttctcaat gatttgaata gtattcgtta tcaactaact
    30781 ttggaacaat atcaggtgac cttgcaagtg agcaagtcac agggatacca ccttcatgcc
    30841 caccctctta ataaaattca gtatcttcaa tcgcttctat atcatatttt tatggaagaa
    30901 aatgccactt ttgtatctat tttagaagat aagatgaaag agaggttaga tgatgagtgt
    30961 ttgctttctg ttgaaatgaa ccaatttttt aaggaacagg ttcctttagt tgaacaagat
    31021 ttagggaaga aaataaacca tcatgaaata acttttatgt tgcaggttct accttatttg
    31081 ctgttaagct gtcataatgt tgaacagtat caagaaagac atcaggatat agagaaagaa
    31141 ttttctttga taagaaaaag aatagagtat caggtgtcta agaaattagg agaacggttg
    31201 tttcaaaatt ttgaaatttc tttgtcagga cttgaagttt ctcttgtagc tgttctcctc
    31261 ctctcctatc gtaaagattt ggatattcat gcagaaagtg atgattttcg gcaattaaaa
    31321 cttgctttag aagaatttat ctggtatttt gaatcacaaa tccgaatgga gattgagaac
    31381 aaggatgatt tgttacgaaa tttgatgatc cactgtaaag ccttgttatt tagaaagact
    31441 tacggtattt tttctaaaaa tcctctaaca aaacaaattc gatccaagta tggagaatta
    31501 tttttagtca ctagaaaatc tgcggaaatt ttagaaggag catggtttat tcggctaaca
    31561 gacgatgata ttgcctattt gacgattcat attggaggat ttttaaagta tacaccatcg
    31621 tctcaaaaaa atatgaaaaa agtttatctc gtttgtgatg aaggtgttgc ggtttcgaga
    31681 cttttgctga aacaatgcaa actttatttt ccaaatgagc aaattgacac tgtatttaca
    31741 acagaacaat ttaagagtgt ggaagatatt gcacaagttg atgtagtgat tactactaat
    31801 gatgatttgg atagcagatt tccgatttta agggttaatc ctatccttga agcagaagat
    31861 attttgaaaa tgctagacta tcttaaacac aatatatttc gtaataagag caaaagtttc
    31921 agtgaaaatc tttctagtct tatttcgtct tatattgtag acagcaagtt ggctagtaag
    31981 ttccaagaag aggttcaaac acttataaat caagaaatag tagttcaagc ttttttggaa
    32041 gatatttgaa ggacagtcca atgatgaaca caaacctgtg tttttcttgg tcttttttag
    32101 tgttttgaag ggtggtatac taatctcaaa gataacaatt atatccaaag gaggcaacat
    32161 atgccaaacg tcaaagaaat tacaagagag tcatggattt tagccacttt cccagagtgg
    32221 ggaacatggt tgaacgaaga aatcgaagaa gaagtcgtac ctgaaggcaa ctttgccatg
    32281 tggtggctag gcaactgtgg tacttggatt aagacaccag ctggtgctaa cgttgtcatg
    32341 gacctttggt caaaccgtgg aaaatcaacc aaaaaagtga aagatatggt tcgtgggcac
    32401 caaatggcaa atatggcagg tgttcgtaag ttgcaaccaa acttgcgtgt tcagccaatg
    32461 gttatcgatc catttgctat caacgaacta gactattact tagtttcaca cttccacagt
    32521 gatcatatcg acccatacac agctgcagca attctcaaca atcctaagtt agagcatgtt
    32581 aagtttatcg gtccttacca ctgtggacga atctgggaag gatggggtgt tccaaaagaa
    32641 cgtatcatcg ttgttaaacc aggtgacact atcgaattaa aagatatgaa gattcatgca
    32701 gtagaatcat ttgaccgtac ttgcttggta actctcccag tgaacggtgc tgatgagaca
    32761 ggcggtgaac ttgctggctt ggctgttaca gatgaagaaa tggctcaaaa ggctgttaac
    32821 tatatctttg aaacaccagg tggaaccatc tatcatggtg cagattctca cttctcaaac
    32881 tattttgcaa aacatggtaa agactttaaa attgatgttg ctttgaataa ctatggtgaa
    32941 aatccggtag gtatccaaga caaaatgaca tctatcgacc ttcttcgtat ggcagaaaat
    33001 ctgcgtacca aagtcattat cccagttcac tatgatatct ggtctaactt catggcttct
    33061 actaatgaga ttctagaact ttggaaaatg cgaaaagatc gcttgcaata cgatttccat
    33121 ccatttatct gggaagttgg cggtaagtac acttatcctc aagatcaaca cttagtagaa
    33181 taccatcatc cacgtggttt tgatgattgt tttgaacaag actctaacat tcaatttaaa
    33241 gctttgctat aatataaaaa tattctttgg aggttatccg tatgttgcat gatacggata
    33301 attttcatat aatagtaaaa tagaatgtgt gattcaataa tcacctcaaa tagaaaggaa
    33361 attctatgtc aaatctatct gttaatgcaa ttcgttttct aggtattgac gccattaata
    33421 aagccaactc aggtcatcca ggtgtggtta tgggagcggc tccgatggct tacagcctct
    33481 ttacaaaaca acttcatatc aatccagctc aaccaaactg gattaaccgc gaccgcttta
    33541 ttctttcagc aggtcatggt tcaatgctcc tttatgctct tcttcatctt tctggttttg
    33601 aagatgtcag catggatgag attaagagct tccgtcaatg gggttcaaaa acaccaggtc
    33661 acccagaatt tggtcatacg gcagggattg atgctacgac aggtcctcta gggcaaggga
    33721 tttcaactgc tactggtttt gcccaagcag aacgtttctt ggcagccgaa tataaccgtg
    33781 aaggctacaa tatctttgac cactatactt acgttatctg tggagacgga gacttgatgg
    33841 aaggtgtctc aagcgaggca gcttcatacg caggcttgca aaaacttgat aagttggttg
    33901 ttctttatga ttcaaatgat atcaacttgg atggtgagac aaaggattcc tttacagaaa
    33961 gtgttcgtga ccgttacaat gcctacggtt ggcatactgc cttggttgaa aatggaacag
    34021 acttggaagc catccatgct gctatcgaaa cagcaaaagc ttcaggaaag ccatctttaa
    34081 ttgaagtgaa gacggttatt ggatatggtt ctccaaacaa acaaggaact aatgctgtac
    34141 acggcgcccc tcttggagca gatgaaactg catcaactcg ccaagccctc ggttgggact
    34201 acgaaccatt tgaaattcca gaacaagtat atgctgattt caaagaacat gttgcagacc
    34261 gtggcgcatc agcttatcaa gcttggacta aattagttgc agattataaa gaagctcatc
    34321 cagaactggc tgcagaagta gaagccatca tcgacggacg tgatccagtc gaagtgactc
    34381 cagcagactt cccagcttta gaaaatggtt tttctcaagc aactcgtaat tcaagtcaag
    34441 atgccttgaa tgttgtggca gctaaattac caaccttcct aggtggatca gctgaccttg
    34501 ctcactcaaa catgacttat atcaagacgg atggacttca agacgatacc aatcgcttga
    34561 atcgtaacat tcagtttggt gttcgtgaat ttgcaatggg aacaatcttg aacgggatgg
    34621 cccttcatgg tggacttcgt gtatacggtg gaactttctt cgtcttctct gactatgtga
    34681 aggcagctgt ccgcttgtca gccttacaag gacttcctgt gacttatgtc tttacccatg
    34741 attcaatcgc agttggggaa gatggtccga ctcacgaacc agttgagcat ttagcaggtc
    34801 ttcgtgctat gccaaatcta aatgttttcc gtccagcaga tgcgcgtgaa acgcaagcag
    34861 cttggtacct tgcagtgaca agtgagaaaa caccaactgc ccttgtcttg acacgtcaaa
    34921 acttgactgt tgaagatgga acagacttcg acaaggttgc taaaggtgct tatgttgtat
    34981 atgaaaatgc agccgacttt gataccatct tgattgcgac aggttcagag gttaatcttg
    35041 ctgtctcagc tgctaaagaa ttggctagtc aaggcgaaaa aatccgcgta atcagcatgc
    35101 catctacaga tgtctttgat aaacaaaatg cagcttacaa ggaagagatt cttccaaatg
    35161 cagtccgtcg tcgtgttgca gtcgaaatgg gtgcaagtca aaactggtac aaatatgttg
    35221 gtctcgatgg tgccgttcta ggtattgata ccttcggagc ctctgcccca gcaccaaaag
    35281 tattggcaga atatggcttt actgtagaaa atcttgtaaa aattgttcga aacttgaaat
    35341 aatcctaaaa atcagggcgt aagctctggt ttttcttgcc agaaaagtaa ggtacaatct
    35401 tgtaaaagta gctgaaattt gatatagtag tcctatgtaa aagacaaagg agaaaacaat
    35461 gaatccaaat attacttttt taatcatgct tgtaggtatg atggccttga tgttctttat
    35521 gcaacgttct caaaagaaac aagctcaaaa gcgtatggaa agcttaaata aactacaaaa
    35581 aggctatgaa gtgattacaa tcggtggact ttatggaaca gtcgatgaag tagatacgga
    35641 gaagggaaca atagttcttg acgtagatgg agtttacttg acttttgaac tagctgctat
    35701 caagacagta ttaccgctga aagaaacagc ttcactagaa ggcgcaattg aaaaataaga
    35761 cgggatcacg aactcccgtt tttctataaa agaaaggaaa tgggatgaaa aaattagtct
    35821 ttgtctgtct gggaaatatt tgccgtagcc ctatggccga gtttgttatg aaatcaatga
    35881 cagataacta cgaaatccaa agtcgagcaa cttcctcttg ggaacatggc aatccgattc
    35941 ataaggggac tcagggaatt tttcaagagt atgagattcc ttatgacaag aacaagacat
    36001 cgcttcagat tagtaaggaa gattttgaag cctttgatta tattatcgga atggacgctt
    36061 caaatatttc tgacttacgt cagatgtgtc cagtagactg tcaagataag atttactcat
    36121 tttcatctga aagtgttcca gacccttggt atacaggaga ttttgaagaa acctatcgac
    36181 gtgttcaaga gggctgtcaa gcttggttag aacgtttaga aaaggagagt taaggtggaa
    36241 aatcttacga atttttacga aaagtatcgt gtctatctga ctcgtccacg tttagagctt
    36301 ttggcagtag ttaccattgt tttctgtgct gtactcgtct tttttctaaa tattccagga
    36361 aaaggtgtct taaaactcga taatggaacg attgtttatg atggcagtct tgtccgtggt
    36421 aaaatgaatg gccaaggtac cattaccttc caaaatggag accaatatac aggtggcttc
    36481 aacaatggag ccttcaacgg aaaaggtacc tttcaatcta aagaaggctg gacctacgaa
    36541 ggtgattttg taaatggtca ggctgaagga aaagggaaac taacaacaga acaagaagtc
    36601 gtttatgaag gaacttttaa acaaggcgtt tttcaacaaa aataaagcct ccttatcaaa
    36661 ggaggtatta ttagaatcac aaggtaagcg cttacctgta aatccctttc tttccaaatc
    36721 cctcttccaa gcaagtttgt gaaataaaaa atatttgaaa taaatttcac aaacttcaaa
    36781 gataaaactt gataagaaaa gaaaatgaga aaagtttcac aagagtttaa aaattctttg
    36841 tgaaatgttt atgaaactgt ttacaaagtt gaaataatgc gttataataa acttgtgaaa
    36901 aaattaacaa aggatatcgt tccttgaaag ctatggagga aaatatggct gataaaaaaa
    36961 ctgtgacacc agaggaaaag aaactcgttg ctgaaaaaca cgtagatgag ttggttcaaa
    37021 aagctctagt tgcccttgaa gaaatgcgta aattggatca agaacaagtt gactacatcg
    37081 ttgccaaagc atcagtagca gctttggatg cccacggaga attggcttta catgcctttg
    37141 aagaaacagg acgtggtgta tttgaagaca aagcaactaa gaacttgttt gcctgtgaac
    37201 acgtagtaaa caacatgcgc cacactaaga cagttggtgt tatcgaagaa gacgatgtaa
    37261 caggattgac tcttattgct gaaccagttg gtgttgtttg tggtattact ccaacaacaa
    37321 acccaacatc aacagcaatc ttcaaatcat tgatttcatt gaagacacgt aacccaatcg
    37381 tctttgcctt ccatccatca gcacaagaat catctgctca tgcagctcgt atcgtccgcg
    37441 atgcagctat cgcagctggt gctcctgaaa actgtgtgca atggattact caaccatcta
    37501 tggaagcaac aagtgccctt atgaaccacg aaggtgttgc gacaatcctt gcaacaggtg
    37561 gtaatgccat ggttaaggcg gcttattcat gtggtaaacc agctcttggg gtaggtgccg
    37621 gaaacgttcc agcttatgtt gaaaaatcag caaacattcg tcaagcagca cacgatatcg
    37681 tcatgtctaa atcatttgat aacggtatgg tctgtgcatc tgaacaagca gttatcattg
    37741 ataaagaaat ttacgatgaa tttgtagcag agttcaaatc ttaccacact tactttgtaa
    37801 acaaaaaaga aaaagctctt cttgaagagt tctgcttcgg tgtcaaagca aacagcaaaa
    37861 actgtgctgg tgcaaaattg aacgctgaca tcgttggtaa accagcaact tggattgcag
    37921 aacaagcagg atttacagtt ccagaaggaa caaacattct tgctgcagaa tgtaaagaag
    37981 ttggcgaaaa tgagccattg actcgtgaaa aattgtcacc agttattgca gttttgaaat
    38041 ctgaaagccg tgaagatggt attactaaga ctcgtcaaat ggttgaattt aacggtcttg
    38101 gacactcagc agctatccac acagctgacg aagaattgac taaagaattt ggtaaagctg
    38161 ttaaagctat tcgtgttatc tgtaactcac cttctacttt tggtggtatc ggggacgttt
    38221 acaatgcctt cttgccatca ttgacacttg gatgtggttc ttacggacgc aactcagttg
    38281 gggataacgt tagtgccatt aacctcttga atatcaaaaa agtcggaaga cggagaaata
    38341 acatgcaatg gatgaaactt ccttcaaaaa catactttga acgtgattca attcaatacc
    38401 ttcaaaaatg tcgtgacgtt gaacgtgtca tgatcgttac tgaccatgcc atggtagagc
    38461 ttggtttcct tgatcgtatc atcgaacaac tggaccttcg tcgcaataag gttgtttacc
    38521 aaatctttgc ggatgtagaa ccggatccag atatcacaac tgtaaaccgt ggtactgaga
    38581 ttatgcgtgc cttcaaacca gataccatca tcgcactcgg tggtgggtct ccaatggatg
    38641 ctgccaaagt aatgtggctt ttctacgagc aaccagaagt ggacttccgt gaccttgtcc
    38701 aaaaattcat ggatatccgt aaacgtgcct tcaagttccc attgcttggt aagaagacta
    38761 aattcatcgc gattccaact acatctggta caggatctga agtaacacca tttgccgtta
    38821 tctctgataa agcaaacaac cgtaaatacc caattgctga ctactcattg acaccaactg
    38881 tggcaatcgt agaccctgct ttggtattga cagttccagg atttgttgct gctgatactg
    38941 gtatggacgt attgactcac gcaacagaag catatgtatc acaaatggct agtgactaca
    39001 ctgatggttt agcacttcaa gccattaaat tggtctttga aaatctcgaa agctcagtta
    39061 agaatgcaga cttccactca cgtgagaaaa tgcataacgc ttcaacaatc gctggtatgg
    39121 cctttgccaa tgccttctta ggtatttctc actcaatggc ccataagatt ggtgcgcaat
    39181 tccacacaat ccacggtcgt acaaatgcta tcttgcttcc gtacgttatc cgttacaatg
    39241 gtacacgtcc agctaagaca gcaacatggc ctaagtacaa ctattaccgt gcagatgaaa
    39301 aataccaaga tatcgcacgc atacttggac ttccagcttc tactccagaa gaaggggttg
    39361 aatcttacgc aaaagctgtc tacgaactcg gtgaacgtat tgggatccaa atgaatttta
    39421 gagaccaagg aattgacgaa aaagaatgga aagaacattc tcgtgaatta gccttcctgg
    39481 cttatgaaga ccaatgttca ccagctaacc cacgtcttcc aatggtagac catatgcaag
    39541 aaatcatcga agatgcatac tatggctaca aagaaagacc aggacgccgt aaataattgt
    39601 ttatcagtct agaagcaaga caaaaactca atttgaggga aagatccagt aatttttcta
    39661 tgataaaagg catcctatca aggtttttga acacctgata ggatgccttt ttatgatatt
    39721 gaggcctttt tgcccttttt gaaaaactag aatagaaaca aaatatataa tagattgaaa
    39781 ctagaatagt acacatctgc ttctaaaaca ttgttagaat tcgatttgac tgtcctgatc
    39841 gatt
//
LOCUS       DBEROU010000017        39531 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.017, whole genome shotgun sequence.
ACCESSION   DBEROU010000017 DBEROU010000000
VERSION     DBEROU010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 39531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39531
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.017"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            308..2461
                     /gene="comA"
                     /locus_tag="ACL8CL_000926"
     CDS             308..2461
                     /gene="comA"
                     /locus_tag="ACL8CL_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter ComA"
                     /protein_id="HGS1330196.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLIANIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLIALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2474..3823
                     /gene="comB"
                     /locus_tag="ACL8CL_000927"
     CDS             2474..3823
                     /gene="comB"
                     /locus_tag="ACL8CL_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence pheromone export protein ComB"
                     /protein_id="HGS1330197.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQAEIGNLISQTEAKIRDYQTAKSAIETGTSLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            3993..4700
                     /gene="purC"
                     /locus_tag="ACL8CL_000928"
     CDS             3993..4700
                     /gene="purC"
                     /locus_tag="ACL8CL_000928"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HGS1330198.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKEQIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            4757..8482
                     /locus_tag="ACL8CL_000929"
     CDS             4757..8482
                     /locus_tag="ACL8CL_000929"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HGS1330199.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLVEDLFAPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITIGIAKQEFS
                     ESDKTIPKLPFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTATHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDTFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDINGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTIFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAVGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEDANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8575..10017
                     /gene="purF"
                     /locus_tag="ACL8CL_000930"
     CDS             8575..10017
                     /gene="purF"
                     /locus_tag="ACL8CL_000930"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HGS1330200.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGTGAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAVCSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSMTYLSIDGLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKTSFYK"
     gene            10054..11076
                     /gene="purM"
                     /locus_tag="ACL8CL_000931"
     CDS             10054..11076
                     /gene="purM"
                     /locus_tag="ACL8CL_000931"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HGS1330201.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLVSSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11073..11618
                     /gene="purN"
                     /locus_tag="ACL8CL_000932"
     CDS             11073..11618
                     /gene="purN"
                     /locus_tag="ACL8CL_000932"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HGS1330202.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            11702..12211
                     /locus_tag="ACL8CL_000933"
     CDS             11702..12211
                     /locus_tag="ACL8CL_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HGS1330203.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12236..13783
                     /gene="purH"
                     /locus_tag="ACL8CL_000934"
     CDS             12236..13783
                     /gene="purH"
                     /locus_tag="ACL8CL_000934"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HGS1330204.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     ETIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAANENQIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYTVVLDELSAN
                     GETTYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQAMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDRPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIAVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLDGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYDLTMVFT
                     GVRHFRH"
     gene            13905..15167
                     /gene="purD"
                     /locus_tag="ACL8CL_000935"
     CDS             13905..15167
                     /gene="purD"
                     /locus_tag="ACL8CL_000935"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HGS1330205.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIENHGAPIVVKADGLALGKGVVVAETV
                     EQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPMAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIKGLFYRTDIGSKAIK"
     gene            15424..15540
                     /locus_tag="ACL8CL_000936"
     CDS             15424..15540
                     /locus_tag="ACL8CL_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HGS1330206.1"
                     /translation="MKERLDDNPIVQGNWKTLGFQFRHETPLVAAAVPHKLR"
     gene            15570..16058
                     /gene="purE"
                     /locus_tag="ACL8CL_000937"
     CDS             15570..16058
                     /gene="purE"
                     /locus_tag="ACL8CL_000937"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009567721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HGS1330207.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVISAHRTPD
                     LMFKHAEEARSRGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDRSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16045..17136
                     /gene="purK"
                     /locus_tag="ACL8CL_000938"
     CDS             16045..17136
                     /gene="purK"
                     /locus_tag="ACL8CL_000938"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HGS1330208.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17146..17373
                     /locus_tag="ACL8CL_000939"
     CDS             17146..17373
                     /locus_tag="ACL8CL_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HGS1330209.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17436..18734
                     /gene="purB"
                     /locus_tag="ACL8CL_000940"
     CDS             17436..18734
                     /gene="purB"
                     /locus_tag="ACL8CL_000940"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HGS1330210.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCDKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(18789..22673)
                     /gene="strH"
                     /locus_tag="ACL8CL_000941"
     CDS             complement(18789..22673)
                     /gene="strH"
                     /locus_tag="ACL8CL_000941"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored beta-N-acetylhexosaminidase StrH"
                     /protein_id="HGS1330211.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTPTTTEN
                     QPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEEV
                     SSTPSDKAEVVTPTSAEKETANKKAEEASPKKDKPAKKDEAKAEADKPATEAGKERAA
                     TVSEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYTDLHLLVGNDGLRFMLDDMS
                     ITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMTDLINYAKDKGIGLIPTVNS
                     PGHMDAILNAMKELGIQNPNFSYFGKESARTVDLDNEQAVAFTKALIDKYAAYFAKKT
                     EIFNIGLDEYANDATDAKGWSVLQADKYYPNEGYPVKGYEKFIAYANDLARIVKSHGL
                     KPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVASSKLLAEKGHQILNTNDAW
                     YYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADIPIIGGMVAAWADTPSARYS
                     PSHLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNRYTAESVAAVKEAEKAIRSL
                     DSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAKREVEKLAKNKVISIDAGRK
                     YFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMTITANGKTYASDDVKKAIIE
                     GTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINSPGHMDAMLVAMEKLGIKNP
                     QAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTKIFNFGTDEYANDATSAQGW
                     YYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYYEDKDDVQFDKDVLISYWSK
                     GWWGYNLASPQYLASKGYKFLNTNGDWYYILGQKPEDGGGFLKKAIENTGKTPFNQLA
                     STKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAFADHNKDYFRANYNALREEL
                     AKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDTLVANLKAALQGLKPAATHS
                     GSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPAGQENIITAGVKGERTHYIS
                     VLTENGKTTETVLDSQVTKEVINQVVEVGAPVTHKGDESGLAPTTEVKPRLDIQKEEI
                     PFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSADGKEVKTLVNSVVAQEAVTQ
                     IVEVGTMVTHVGDENGQAAIAEEKPKLEIPRQPAPSTAPAEESKALPQDPAPVVTEKK
                     LPETGTHDSAGLVVAGLMSTLAAYGLTKRKED"
     gene            complement(22988..23704)
                     /locus_tag="ACL8CL_000942"
     CDS             complement(22988..23704)
                     /locus_tag="ACL8CL_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1330212.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEQFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24052..25839
                     /locus_tag="ACL8CL_000943"
     CDS             24052..25839
                     /locus_tag="ACL8CL_000943"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HGS1330213.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPREGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPAYIEAVGRYYDQLLPRLVPRLLDNGGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKWLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            25836..26312
                     /locus_tag="ACL8CL_000944"
     CDS             25836..26312
                     /locus_tag="ACL8CL_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1330214.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26340..27245
                     /locus_tag="ACL8CL_000945"
     CDS             26340..27245
                     /locus_tag="ACL8CL_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS1330215.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27232..28047
                     /locus_tag="ACL8CL_000946"
     CDS             27232..28047
                     /locus_tag="ACL8CL_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1330216.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28054..28458
                     /locus_tag="ACL8CL_000947"
     CDS             28054..28458
                     /locus_tag="ACL8CL_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1330217.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            28757..29923
                     /locus_tag="ACL8CL_000948"
     CDS             28757..29923
                     /locus_tag="ACL8CL_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HGS1330218.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVAKPAT
                     YLKKDVATVLVSFARSGNSPESVATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTALLVFDPTEFAVKSERFEVVSSLARKVLDKV
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTAYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30039..31076
                     /locus_tag="ACL8CL_000949"
     CDS             30039..31076
                     /locus_tag="ACL8CL_000949"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HGS1330219.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     IYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHIYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            31284..32138
                     /locus_tag="ACL8CL_000950"
     CDS             31284..32138
                     /locus_tag="ACL8CL_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HGS1330220.1"
                     /translation="MTYEYKSHIYLAETVLNVKDLASQTAFYQQVIGLEILSQTETES
                     ILGLGGKVLVQLIQAQESGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEAIYLEDLEGNGIELYRDKPVSTWNIREDGRIIGVTEALAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSSQFYQKVLGLENKFSVPGASWIAAGDYHHHLAVNEW
                     GGKGLASRKQGLPGLAYYVIEVAHKKELLTIAQRAQEVDAPIKWMTSSQLEITDSDGI
                     VTRIRLAR"
     gene            32288..32545
                     /locus_tag="ACL8CL_000951"
     CDS             32288..32545
                     /locus_tag="ACL8CL_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HGS1330221.1"
                     /translation="MTNSKYITCLKRSEGQLCGIQKMIEGDCDCADIVTQLTAVRSSV
                     ERVIEMIITENLTECINQPLDDSEAQKERLEKAIRYLIKRK"
     gene            complement(32587..32949)
                     /locus_tag="ACL8CL_000952"
     CDS             complement(32587..32949)
                     /locus_tag="ACL8CL_000952"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330222.1"
                     /translation="MKIKVQTRKLAAGCSKHCFKVVDGTDEVSSKHCFEVVDRTDEVS
                     NHIYGKVKLTWFEEIFEEYKSLHNKTHITKVLMNCTPKVRQKKSNFLGSVHSTPDTMR
                     FFNFKDFLQLLLTHIKLF"
     gene            complement(33003..33299)
                     /locus_tag="ACL8CL_000953"
     CDS             complement(33003..33299)
                     /locus_tag="ACL8CL_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HGS1330223.1"
                     /translation="METSISMADFYGKYQNENLELIDVREAHEFQAGHAPGAKNLPLS
                     TLEQGYKELKPDHEYYVICQGGVRSASTCQFLSSQGLTVTNVEGGMNAWPGQVE"
     gene            complement(33289..33471)
                     /locus_tag="ACL8CL_000954"
                     /pseudo
     CDS             complement(33289..33471)
                     /locus_tag="ACL8CL_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
     gene            33520..34284
                     /locus_tag="ACL8CL_000955"
     CDS             33520..34284
                     /locus_tag="ACL8CL_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HGS1330224.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVINYKGEEVCLAQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPCRYMEMQPEAIAAIEEVLE
                     DRGIPYEEVMTWTTDGFYRETAEKVAYRKEEGCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            complement(34524..34676)
                     /locus_tag="ACL8CL_000956"
     CDS             complement(34524..34676)
                     /locus_tag="ACL8CL_000956"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330225.1"
                     /translation="MRTKASLRKLVTGCSKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTAR"
     gene            34894..36273
                     /locus_tag="ACL8CL_000957"
     CDS             34894..36273
                     /locus_tag="ACL8CL_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGS1330226.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLV
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            36287..36952
                     /locus_tag="ACL8CL_000958"
     CDS             36287..36952
                     /locus_tag="ACL8CL_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HGS1330227.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            37262..37801
                     /locus_tag="ACL8CL_000959"
     CDS             37262..37801
                     /locus_tag="ACL8CL_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1330228.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGGEQVGILIINDGSQDQT
                     QEIAECLASKYPNIVRAIYQENKCHGGAVNRGLAEASGRYFKVVDSDDWVDPRAYLKI
                     LETLQELESKGQEVDVFVTNFVYEKEGQSRKKSMSYDSVLPVRQIFGWDQVGNFSKGQ
                     YTMMHSLIYRTDLLRASQF"
     gene            37868..38278
                     /locus_tag="ACL8CL_000960"
     CDS             37868..38278
                     /locus_tag="ACL8CL_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HGS1330229.1"
                     /translation="MYYLPVDFYRYLIGREDQSVNEQVMIKCIDQQLKVNRLLIDQLD
                     LSQVSHPKMREYLLNHIELTTVISSTLLNRSGTAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            38394..>39531
                     /gene="pavB"
                     /locus_tag="ACL8CL_000961"
     CDS             38394..>39531
                     /gene="pavB"
                     /locus_tag="ACL8CL_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HGS1330230.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKLEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAVKDNVKDSIDVPAAYLEKAKGEGPFTAGVN
                     HVIPYELFAGDGMLTRLLLKASDKAPWSDNGDAKNPALSPLGENV"
BASE COUNT        10860 a         7717 c         9269 g        11685 t
ORIGIN      
        1 tattttctag gtagaacgtt tgttctgcct atttttttat ccaaaaagtg cagttgggag
       61 ggagataggc tcatttggga aggaagtcca gtttttgttt agtgattggg gtaagatagt
      121 tgttatcaga tgagtttata ctcttcgaaa atcaaattca aaccacgtca acgtcgcctt
      181 gccgtatata tgtgactgac ttcgtcagtc ctatctacaa cctcaaaaca gtgttttgag
      241 cagcctgcgg ctagtttcct agtttgctct ttgattttca ttgagtatta gggaaaagga
      301 gatgaatatg aaatttggga aacgtcacta tcgtccgcaa gtggatcaga tggactgcgg
      361 tgtagcttca ttagccatgg tttttggcta ctatggtagt tattattttt tggctcactt
      421 gcgagaattg gctaagacga ccatggatgg gacgacggct ttgggcttgg tcaaggtggc
      481 agaggagatt ggttttgaga cgcgagccat taaggcggat atgacgcttt ttgacttgcc
      541 ggatttgact tttccttttg ttgcccatgt gcttaaggaa gggaaattgc tccactacta
      601 tgtggtgact gggcaggata aggatagcat tcatattgcc gatccagatc ccggggtgaa
      661 gttgactaaa ctgccacgtg agcgttttga ggaagaatgg acaggagtga ctctttttat
      721 ggcacctagt ccagactata agcctcataa ggaacaaaaa aatggtctgc tctcttttat
      781 ccctatatta gtgaagcagc gtggcttgat tgccaatatc gttttggcaa cactcttggt
      841 aaccgtgatt aacattgtgg gttcttatta tctgcagtct atcattgata cctatgtgcc
      901 agatcagatg cgttcgacac tagggattat ttctattggg ctagtcatcg tctacatcct
      961 ccagcaaatc ttgtcttacg ctcaggagta tctcttgctt gttttggggc aacgcttgtc
     1021 gattgacgtg attttgtcct atatcaagca tgtttttcac ctccctatgt ccttctttgc
     1081 gacacgcagg acaggggaga tcgtgtctcg ttttacagat gctaacagta tcatcgatgc
     1141 gctggcttcg accatccttt cgattttcct agatgtgtca acggttgtca ttatttccct
     1201 tgttctattt tcacaaaata ccaatctctt tttcatgact ttattggcgc ttcctatcta
     1261 cacagtgatt atctttgcct ttatgaagcc gtttgaaaag atgaatcggg ataccatgga
     1321 agccaatgcg gttctgtctt cttctatcat tgaggacatc aacggtattg agactatcaa
     1381 gtccttgacc agtgaaagtc agcgttacca aaaaattgac aaggaatttg tggattatct
     1441 gaagaaatcc tttacctata gtcgagcaga gagtcagcaa aaggctctga aaaaggttgc
     1501 ccatctcttg cttaatgtcg gcattctctg gatgggggct gttctggtca tggatggcaa
     1561 gatgagtttg gggcagttga ttacctataa taccttgctg gtttacttta ccaatccttt
     1621 ggaaaatatc atcaatctgc aaaccaagct tcagacagcg caggttgcca ataaccgtct
     1681 aaatgaagtg tatctagtag cttctgagtt tgaggagaag aaaacagttg aggatttgag
     1741 cttgatgaag ggagatatga ccttcaagca ggttcattac aagtatggct atggtcgaga
     1801 cgtcttgtcg gatatcaatt taaccgttcc ccaagggtct aaggtggctt ttgtggggat
     1861 ttcagggtca ggtaagacga ctttggccaa gatgatggtt aatttttacg acccaagtca
     1921 aggggagatt agtctgggtg gtgtcaatct caatcagatt gataaaaaag ccctgcgcca
     1981 gtacatcaac tatctgcctc aacagcccta tgtctttaac ggaacgattt tggagaatct
     2041 tcttttggga gccaaggagg ggacgacaca ggaagatatc ttacgggcgg tcgaattggc
     2101 agagattcga gaggatatcg agcgcatgcc actgaattat cagacagaat tgacttcgga
     2161 tggggcaggg atttcaggtg gtcaacgtca gagaatcgct ttggcgcgtg ctctcttgac
     2221 agatgcgccg gtcttgattt tggatgaggc gactagcagt ttggatattt tgacagagaa
     2281 gcggattgtc gataatctca ttgctttgga caagaccttg attttcattg ctcaccgctt
     2341 gactattgct gagcggacag agaaggtggt tgtcttggat cagggcaaga ttgtcgaaga
     2401 aggaaagcat gctgatttgc ttgcacaggg aggcttttac gcccatttgg tcaatagcta
     2461 gaaagaggag aggatgaaac cagaattttt agaaagtgcg gagttttata atcgtcgtta
     2521 ccataatttt tccagtagtg tgattgtacc catggccctt ctgctcgtgt ttttacttgg
     2581 ctttgcaact gttgcagaga aggagatgag tttgtccact agagctactg tcgaacccag
     2641 tcgtatcctt gcaaatatcc agtcaactag caacaatcgt attcttgtca atcatttgga
     2701 agaaaataag ctggttaaga agggggatct tttggttcaa taccaagaag gggcagaggg
     2761 tgtccaagcg gagtcctatg ccagtcagtt ggacatgcta aaggatcaaa aaaagcaatt
     2821 ggagtatctg caaaagagcc tgcaagaagg ggagaaccac tttccagagg aggataagtt
     2881 tggctaccaa gccacctttc gcgactacat cagtcaagca ggcagtctta gggctagtac
     2941 atcgcaacaa aatgagacca tcgcgtccca gaatgcagca gctagccaaa cccaagccga
     3001 aatcggcaac ctcatcagtc aaacagaggc taaaattcgc gattaccaga cagctaagtc
     3061 agctattgaa acaggtactt ccttggccgg tcagaatcta gcctactctc tttaccagtc
     3121 ctacaagtct cagggcgagg aaaatcccca aactaaggtt caggcagttg cacaggttga
     3181 agcacagatt tctcagttag aatctagtct tgctacttac cgtgtccagt atgcaggttc
     3241 aggtacccag caagcctatg cgtcagggtt aagcagtcaa ttggaatccc ttaaatccca
     3301 acacttggca aaggttggtc aggaattgac ccttctagcc cagaaaatct tggaggcaga
     3361 gtcaggtaag aaggtacagg gaaatctttt agacaagggg aaaattacgg caagtgagga
     3421 tggggtgctt catcttaatc ctgagaccag tgattctagc atggttgcag aaggtgccct
     3481 actagcccaa ctttatccat ctttggaaag agaagggaaa gccaaactca cagcctatct
     3541 aagttcaaaa gatgtagcaa gaatcaaggt cggtgattct gttcgctata ctacgactca
     3601 tgatgccggg aatcaacttt ttctagattc tactattaca agtattgatg cgacagctac
     3661 taagactgag aaagggaatt tctttaaaat cgaggcggag actaatctaa cttcggagca
     3721 ggctgaaaaa cttaggtacg gggtggaagg ccgtctacag atgattacgg gcaagaaaag
     3781 ttatctacgt tattatttgg atcaattttt gaacaaagag taatgttcgt gtttttagag
     3841 ttaaataatt tttaaactgt gagaaagatt cttcttgcag ttttttcttt acaatttttg
     3901 aaaaacatct gctatttatt cggttaaatt cttgtgtttt ttggtttttt gtggtaaaat
     3961 gtgctcaagt aatacgaaag gcgaacttta aaatgtcaaa acaattgatc tattcgggaa
     4021 aagctaaaga tatctataca actgaggatg aaaatcttat tatttcaact tacaaggacc
     4081 aggcgactgc tttcaatggt gtcaagaagg agcagattgc aggtaaggga gtcttgaata
     4141 atcagatttc atcttttatt tttgagaaat taaatgcggc tggtgtggcg actcactttg
     4201 tggagaaact ttcagacacg gaacaactca ataaaaaggt taagattatt cctttggaag
     4261 tcgtgctccg caactatact gctggttcct tttcaaaacg ttttggtgtg gatgagggaa
     4321 tcgccttgga gactccgatt gtcgaatttt actacaaaaa tgatgatttg gatgatccat
     4381 ttatcaatga tgagcatgtg aaattcctac agattgcgga tgaccagcag attgcctact
     4441 tgaaggaaga aacgcgtcgt atcaatgaac tattgaaagt ctggtttgct gagattgggc
     4501 ttaaattgat tgactttaag ctagagttcg gttttgacaa ggatggcaag attatcttgg
     4561 cagacgaatt ttcaccagat aactgccgct tgtgggatgc tgatggcaac cacatggata
     4621 aggatgtttt ccgtagagga ttgggagaac taaccgacgt ttatgagatt gtttgggaaa
     4681 agttgcaggg tttaaaataa caacctcaag gctgtttggg aatattgcaa gagctgaaat
     4741 aaaggaataa gaattgatgg ataaacgtat ttttgttgaa aaaaaggctg attttcaggt
     4801 caagtcagag agtttggtta gagagctcca gcacaacttg ggactgtcaa gcttgaaaag
     4861 tattcgtatt gtgcaagtat atgatgtatt tgacttggtt gaggacttgt ttgcacctgc
     4921 agagaagcac attttctctg agcaggtaac cgaccatgtt ttagatgaag tatctgtgca
     4981 ggcggatctt gctaactatg ctttctttgc cattgaaagt ctaccagggc agtttgacca
     5041 gcgtgcagct tcgtcacagg aagccttgct tttgttggga agttcgagtg acgtgacagt
     5101 caacacagcc caactttact tggtgaataa agatattgat gcgactgagt tggaagctgt
     5161 caaaaactac ctgctcaatc cagttgattc tcgtttcaag gatatcacga tagggattgc
     5221 caagcaggag ttttcagagt cagacaagac cattccaaaa ttgcctttct ttgaaagcta
     5281 tacagcagaa gactttgctc gctacaaggc cgaacaaggg atggccatgg aagtggatga
     5341 tttgctcttt atccaagact actttaagtc aatcgggcgc gtgccaactg agactgaact
     5401 caaggttttg gacacttact ggtctgacca ctgccgtcat acgacttttg agacagagtt
     5461 gaaacacatc gacttttcag cttctaaatt tcaaaagcaa ttgcagtcaa cctatgacaa
     5521 gtatattgcc atgcgcgagg aattaggtcg gtctgaaaaa ccacaaacct tgatggatat
     5581 ggcgactatt ttcggtcgtt atgagcgtgc taatggacga ttggatgata tggaagtctc
     5641 tgacgaaatc aatgcctgct cagttgaaat tgaagtggac gttgatggtg tcaaggaacc
     5701 ttggctcctc atgtttaaaa acgaaactca caaccatcca acagaaattg agccatttgg
     5761 tggagcggct acctgtatcg gtggagctat tcgtgatccg ttgtcaggcc gttcctatgt
     5821 ttaccaagcc atgcgtattt caggtgctgg tgatattaca gcaccgattt cggaaactcg
     5881 tgctgggaaa ttgccacaac aagtcatttc taaaacagca actcatggtt attcttcata
     5941 tggtaaccag attgggcttg ccacaaccta cgttcgtgaa tacttccacc caggctttgt
     6001 agctaaacgt atggaacttg gtgccgttgt tggtgcggct cccaagggca atgttgtccg
     6061 tgaaaaacct gaagcaggtg atgtgatcat ccttctcgga ggtaaaacag gtcgtgatgg
     6121 tgtcggtggt gcgacgggct cttctaaggt tcaaacagtt gagtctgtag agactgctgg
     6181 tgctgaggtt caaaaaggaa atgccatcga agaacgcaag attcagcgcc tcttccgtaa
     6241 tggcaatgtc actcgtctga tcaagaagtc caatgacttt ggggcaggcg gtgtctgtgt
     6301 ggctatcggt gaattggcag acggtcttga aatcgacctc aacaaggtgc ctcttaaata
     6361 ccagggcttg aatggtacag aaattgccat ctctgaatca caagaacgga tggcggtcgt
     6421 ggttcgtcct gaagatgtgg ataccttcgt tgccgaatgt aacaaagaaa atattgatgc
     6481 tgttgtggtg gcgacagtaa ctgaaaaacc aaatcttgtc atgcactgga atggtgagac
     6541 aatcgttgac ttggagcgtc gtttccttga catcaatggt gtgcgcgtgg ttgtcgatgc
     6601 caaagttgtg gacaaggatg tcaaactccc agaagagcgt caaacatctg ctgaaacact
     6661 ggaatcagat acccttacgg ttctatctga cctcaaccat gcaagtcaaa aaggattaca
     6721 gactatcttt gactgctctg ttggacgctc aacggttaat cacccacttg gtggtcgtta
     6781 ccaactcaca ccaactgagg catctgtgca gaaattgcca gttcaacacg gtgtgactca
     6841 tactgcgtcg gtcattgctc aaggtttcaa cccatatgta gctgaatggt ctccatacca
     6901 cggtgctgct tatgcggtta tcgaagcaac tgctcgtttg gtggctgttg gtgccaactg
     6961 gtccaaggct cgtttctctt accaagaata tttcgagcgt atggacaagc aagcagagcg
     7021 ttttggtcag ccagtagccg ctcttctagg ctctattgaa gctcagattc aacttggctt
     7081 gccatctatc ggtggtaagg actccatgtc tggtaccttt gaagaattga ccgttccgcc
     7141 aaccttggtt gcctttgggg tgacgacggc agatagccgt aaggtgctct ctccagaatt
     7201 taaagctgtt ggggaaaata tctactacat cccaggtcaa gccctctctg cagagattga
     7261 ttttgacttg attaagaaaa attttgctca gtttgaagca agccaagctg accataaagt
     7321 gacatctgca tcagctgtca aatacggtgg tgtagttgaa agtttggctc ttgctacctt
     7381 tggaaactat attggtgcag aggtgacctt gcctgaactt aaaacagctt tgacagctca
     7441 attaggcggc tttgtcttca catctcctga agaaattgct ggagtagaga agattggaca
     7501 aacgaaagca gactttacac tgactgtcaa cggtgtgaag ctagatggac acaagcttga
     7561 cagtgcattt caagggacat tggaagaagt ttacccaaca gaatttaccc aagcgaaaga
     7621 actagaagaa gtaccagctg tggcatcaga tgttgtgatt aaagccaaag aaaaggttga
     7681 aaaacctgtg gtttacatcc cagtctttcc aggaaccaac tcagaatatg attcagctaa
     7741 ggccttcgaa aaagaaggtg cagaggtcaa tttggtgcca ttcgtgacct tgaatgaaga
     7801 agctattgtc aagtcagttg aaactatggt tgacaatatc gacaagacta atattctctt
     7861 ctttgctggt ggattctcgg ctgcggatga accagatggt tcagctaagt ttatcgtcaa
     7921 tatcctgctt aatgaaaaag tgcgtgtggc tattgatagc tttatcgccc gtggtggttt
     7981 gattatcggt atttgtaatg gattccaagc cttagtcaaa tcgggtctcc taccctacgg
     8041 aaactttgaa gatgctaaca atactagccc aaccctcttc tacaatgatg ccaaccaaca
     8101 cgtggccaag atggtggaaa ctcgcattgc caataccaac tcaccatggt tggctggtgt
     8161 gcaagtgggc gatatccacg ctattcctgt ttcgcacggt gaagggaagt ttgtcgtgac
     8221 ggctgaggaa tttgcagagc tccgtgacaa tggacaaatt ttcagccaat acgttgactt
     8281 taacggtaaa ccaagtatgg attctaagta caatccgaat ggttctgtcc atgccatcga
     8341 aggaattacc agcaagaatg gtcaaatcat cggtaagatg ggccattcag aacgttatga
     8401 ggacggtctt ttccaaaata tcccaggcaa taaagaccag cacctgttcg cgtcagcggt
     8461 taaacatttc actggaaaat aagacttaca gattttctaa tagatagtat cagtaatgta
     8521 aaagtcatgt aaatctagct cttgatgatt acaaatgaaa attaggtata aaaaatgaca
     8581 tacgaagtaa aatctcttaa tgaagaatgt ggtgttttcg gtatttgggg acatccagat
     8641 gctgctaagt tgacctattt tggactccac agtcttcaac accgtggtca ggagggggca
     8701 ggaatcctct ccaatgatca aggacaactg aagcggcatc gcgatatggg tcttctatca
     8761 gaagtcttca gaaatcctgc taacttagat aaattgacgg gaactggtgc gattgggcat
     8821 gtgcgttacg cgactgctgg cgaagcttct gtagataata tccagccctt cctcttccgt
     8881 tttcacgata tgcagtttgg cttggctcat aatggaaatc tgaccaatgc agcctctctc
     8941 aagaaagaac tggaacaaag aggagcaatt ttcagcgcga cttcggactc tgaaatcttg
     9001 gctcacctca ttcgtcgcag tcataatcct agcctgatgg gcaaaatcaa ggaagcgctc
     9061 agccttgtca aaggtggttt tgcctatatc ttgctgtttg aggacaagtt gattgcggct
     9121 cttgacccaa atggattccg accgctttcg attggtaaaa tggctaatgg agcagttgtt
     9181 gtatcttctg aaacctgtgc ttttgaggtc attggtgccg agtggattcg tgatttgaag
     9241 ccaggtgaga ttgtgatcat tgatgacgag ggcattcagt atgacagcta tacagatgat
     9301 acccagttgg cggtttgttc tatggagtat atctactttg ctcgccctga ttctaatatc
     9361 cacggtgtca atgtccatac ggcacgtaag agaatgggag cgcaattggc gcgagaattt
     9421 aagcatgagg cagatattgt agttggtgtg cccaattctt ccctaagcgc ggctatggga
     9481 tttgcggaag aatcaggctt accaaatgaa atgggtctga tcaaaaacca atacacccag
     9541 cgaactttta tccaaccgac tcaagaattg cgggagcaag gagtgcggat gaaactgtct
     9601 gctgtttcgg gtgttgtcaa aggcaaacgt gtggtcatgg tggatgattc cattgtacgt
     9661 ggaacaacct ctcgtcgtat cgttcagctc ttgaaagaag cgggtgcgac tgaggttcac
     9721 gttgccattg gaagtcctgc actagcgtat ccatgtttct acgggattga tatccagacc
     9781 cgtcaggagc tgattgcagc caatcatacg gtcgaagaaa ctcgccaaat cattggtgcg
     9841 gacagtatga cttatctttc aattgatggc ttgattgagt cgattggtat cgaaacagat
     9901 gcgccgaacg gtggtctctg tgtcgcttac tttgacggtg actacccaac gcctctctat
     9961 gactacgaag aagactatcg tagaagtttg gaagaaaaga ccagttttta caagtaggcg
    10021 acagattctc cattaaagaa aaggaaaaaa caaatgacaa ataaaaatgc atatgcccag
    10081 tcgggtgtgg atgttgaagc gggttatgaa gttgttgaac ggattaaaaa gcacgtggcc
    10141 cgtacggagc gtgcaggtgt catgggagct cttggtggct ttggtggtat gtttgacctt
    10201 tccaagactg gggttaaaga acccgtcttg atttcaggga ctgacggtgt cggaaccaag
    10261 ctcatgttgg ctatcaagta cgacaagcac gataccatcg ggcaggactg tgtggccatg
    10321 tgtgtcaacg acatcattgc tgcaggtgcg gaacccctct attttctcga ctacgtagcg
    10381 acagggaaga atgaaccagc taagctagaa caagtggttg ctggtgtggc agaaggttgt
    10441 gtgcaggctg gtgctgccct catcggtggg gaaacggctg aaatgccggg catgtacggc
    10501 gaagacgact atgacttggc tggttttgcg gtcggtgtgg ctgaaaaatc tcaaatcatt
    10561 gacggttcaa aggtggtaga gggagatgtt cttctcggac ttgtttcaag tgggattcac
    10621 tcaaatggtt actctttggt tcgtcgtgtc tttgcggatt acacaggtga ggaagtccta
    10681 ccagaattgg aaggcaagaa acttaaggaa gttctacttg agccgactcg tatctatgtc
    10741 aaggctgtct tgccgctcat caaagaagag ttggtcaacg gcattgccca catcacaggt
    10801 ggtggcttta tcgaaaatgt ccctcgtatg tttgcagatg acctagctgc tgaaattgat
    10861 gaaagcaaag ttccagtgct tccaattttc aaagcccttg aaaaatatgg tcagattaaa
    10921 cacgaagaaa tgtttgaaat cttcaatatg ggtgtgggac ttatgttggc ggtcagccct
    10981 gaaaatgtag agcgtgtaaa agaattgttg gatgaagcag tctatgaaat tggtcgcatc
    11041 gtcaagaaag aaaacgaaag tgtcattatc aaatgaaaaa aatagcggtt tttgcctctg
    11101 gtaatggctc aaattttcag gtgattgccg aagaatttcc agtggagttt gtcttttcag
    11161 accatcgtga tgcctatgtg cttgagcgtg caaagcagct cggcgttctg tcctatgctt
    11221 ttgaactcaa ggagtttgag agcaagacag actacgaagc agcccttgtc gaactcttgg
    11281 aagaacacca gattgacttg gtttgcctag caggctacat gaaaatcgtt ggaccaacct
    11341 tattgtcggc ttatgaaggt cggattgtca acattcatcc agcctatttg ccagaatttc
    11401 caggagctca tgggattgag gatgcttgga atgctggcgt gggtcagtct ggtgtgacca
    11461 ttcactgggt ggattcgggt gtggatacag gccaggtcat caaacaggtt cgtgtgccac
    11521 gactagctga tgataccatt gacagatttg aagctcgcat ccatgaagca gagtacaggt
    11581 tgtatccgga agtagtgaag gctctattta cagattgact ttttgatgat tcatatgata
    11641 tctttgattt taaattggag tcagtgtttg ttgaagacgg cttcaaacgg aggtatttgt
    11701 aatgttagaa tctaaaaaaa caactcgata tgtattttat gtctatctga tgttattaac
    11761 ttggggaatc ttatttaagt ttgaaacaaa tcctgaattt atagcatttt tcttagctcc
    11821 aaggtatatc aattggattc cattttcaga accactaata gtcgatggaa aaattgtttt
    11881 tgctgaaatg ttatttaatc tgatttcctt tattccatta ggtgtttgtt tccctttgat
    11941 aaaaactaat ttatctagtt taagaatagt cgggacaggt ttcttgatta gtttattgtt
    12001 tgagtgctta cagtatattt tagcaatagg tataacagat ataacggatt tgactttaaa
    12061 tacgctaggt gtctgtgtag gcttactgat ttatcaaatt tttataagag tgttcaaatc
    12121 acagactaga aaatggatca atatcttagg tatgcttagc cttggttttg cttatcttgt
    12181 tttactgtta ctgcatttaa ttggtgttta actaatgatt aaaaaggaga atataatgac
    12241 taaacgcgtc ttaatcagcg tctcagacaa agcgggcatt gttgaatttg cccaagaact
    12301 aaaaaaactt ggttgggaga ttatctcgac aggtggaact aaggttgccc ttgataatgc
    12361 tggggtggag accattgcga tcgatgatgt gacgggcttc ccagagatga tggatggccg
    12421 tgttaagact cttcatccaa atatccacgg gggtcttctc gctcgtcgtg atttggatag
    12481 ccacttggaa gcagccaatg agaaccaaat tgagcttatt gaccttgtag tggtcaacct
    12541 ttatcctttc aaggaaacga ttctcaaacc agacgtgacg tacgctgatg ctgtggagaa
    12601 tattgatatt ggtgggccat ctatgcttcg ttcagcagcg aagaatcatg ccagcgtgac
    12661 ggttgtggta gaccctgctg actatacagt ggttttggac gagttgtcag ccaatggtga
    12721 aacgacttac gaaacgcgtc aacgtttggc agccaaggtt ttccgtcaca cagcggctta
    12781 tgacgctttg attgcagaat acttcacagc tcaagtgggc gaaagcaaac ctgaaaagtt
    12841 gaccttgacc tatgacctaa aacaagctat gcgttatggg gaaaatcccc agcaggatgc
    12901 ggacttctac cagaaagctt tgccgactga ttactccatt gcatcagcca aacagcttaa
    12961 cggtaaggaa ttgtcattca acaatatccg cgatgcagat gctgccattc gtattatccg
    13021 tgacttcaaa gaccgtccaa ccgttgtggc tctcaagcat atgaatccat gtggtatcgg
    13081 ccaggcggat gacatcgaga cggcttggga ttatgcttat gagtctgacc cagtgtctat
    13141 ctttggtggt atcgctgttc ttaaccgtga ggtggatgct gcgacagctg agaagatgca
    13201 cggcgttttc ctcgaaatca tcattgcacc aagctatacg gatgaagcgc tagccatttt
    13261 gatcaataaa aagaaaaact tgcgtatcct tgccttgcca tttaatgctc aagaggctag
    13321 cgaagtggaa gcagaataca caggtgtagt cggtggactt ctcgtgcaaa atcaagacgt
    13381 ggtcaaggaa agcccagctg actggcaagt ggtgaccaaa cgtcagccaa ccgagacaga
    13441 agcgactgct cttgagttcg cttggaaggc tatcaagtac gtcaaatcaa atggtattat
    13501 cgtgaccaac gaccacatga cacttggtgt tggtccaggt caaaccaacc gtgtggcttc
    13561 tgttcgcctt gccattgacc aagccaaaga tcgtctggac ggggcggtcc ttgcttcaga
    13621 tgccttcttc ccatttgcgg ataacgtgga agaaatcgcc aaagcaggaa ttaaggccat
    13681 catccagccc ggtggctctg tccgtgacca agaatccatc gaagcagcgg ataaatacga
    13741 cttgactatg gtctttacag gtgtgagaca ttttagacat taagaagata aaagggaaga
    13801 aaacagtttc tttccttttt tggcttaaaa tgctaactga aacaagatta aaacgaactt
    13861 ttttgatata atgttggtaa ataattcgca aaagaggttg aggaatgaaa ctacttgttg
    13921 tcggttctgg tggtcgtgag catgcgattg ctaaaaagtt acttgaatca aaagacgtgg
    13981 aaaaagtctt tgtagctcct gggaatgatg ggatgactct ggatggtttg gaattggtaa
    14041 atatctctat ttccgaacat tctaaattga ttgacttcgc aaagaccaat gatgttgctt
    14101 ggacctttat cggtccagat gatgcccttg ctgctggtat cgtggatgat tttaaccaag
    14161 ctggacttaa ggcctttggt ccgactaggg ctgcagcgga gctggagtgg tccaaggatt
    14221 tcgccaagga aatcatggtc aaatacggcg ttccgacagc aacatatggc acattttcag
    14281 atttcgagga agccaaggcc tatatcgaaa atcatggtgc gcctatcgta gtcaaggcgg
    14341 atggcttggc acttgggaag ggtgtcgtcg ttgctgagac ggttgagcaa gcggtcgaag
    14401 ccgctcatga gatgcttttg gacaataaat ttggtgactc aggtgcgcgc gtggttattg
    14461 aggaattcct tgaaggagag gaattttcac tctttgcctt tgtaaatggt gataagttct
    14521 acatcatgcc aatggctcag gaccacaaac gtgcctatga tggcgacaaa gggcctaaca
    14581 cgggtggtat gggtgcctat gcgccagtcc cacacttacc acagagtgta gttgatacag
    14641 cggttgacac cattgtcaag ccagttctag aaggggtgat taaagaaggt cgcccttatc
    14701 tgggagttct ttacgcaggg cttatcctga cagctgatgg accgaaagtc attgagttca
    14761 acgctcggtt cggagatcca gaaactcaga ttatcttgcc tcgcttgacc tctgactttg
    14821 ctcaaaatat cacagatatc ctggatagca aggagccaaa tatcatgtgg acggacaagg
    14881 gtgtgactct gggtgtggtt gtcgcatcca agggctaccc gctagactat gaaaggggcg
    14941 ttgagttgcc agcaaagaca gaaggcgatg tcatcaccta ctatgcaggg gctaagtttg
    15001 cggaaaatag cagagcactg ctctcaaacg gcggacgagt ttatatgctc gttaccacag
    15061 cagataccgt caaagaagcc caagccagca tctaccaaga actatcccaa caaaaaataa
    15121 aaggactctt ctaccgaaca gatatcggaa gcaaggcaat taagtaaaga tataagaata
    15181 acgcgccgta gtcgccaaac acgataatgg tcgtcgtggt gaaaagacca gaacagtgaa
    15241 tgttctggtc agggggaaac ttggagacct taggctcaaa gtttaggaat gaaaccgaag
    15301 gtttgcttcc gcctccatca cctaagacca ttatcaaaaa gaaaaataaa aattcacaaa
    15361 atacgttaat gatcgtatgg tttgcgagcg ttagcgagct aatatagaac aatcaccgcc
    15421 gttgtgaaag aacgattgga tgataatcca atcgttcagg gaaattggaa gaccttgggt
    15481 ttccaattta ggcatgagac acctttggtg gctgctgccg tccctcacaa gctaaggtga
    15541 ttgttgaaaa agaggaaaaa ggagaagaaa tgaaaccagt aatttccatc atcatgggct
    15601 caaaatccga ctgggcaacc atgcaaaaaa cagcagaagt cctagaccgc ttcggtgtag
    15661 cctacgaaaa gaaagttatt tccgcacacc gtacaccaga cctcatgttc aaacatgcag
    15721 aagaagcccg tagtcgtggc atcaagatca tcatcgcagg tgctggtggc gcagcgcatt
    15781 tgccaggcat ggtagctgcc aaaacaaccc ttccagtcat tggtgtgcca gtcaagtctc
    15841 gtgctcttag tggagtggat tcactctatt ctatcgttca gatgccgggt ggggtgcctg
    15901 ttgcgaccat ggctatcggt gaagctggag cgactaacgc agctctcttt gccctccgtc
    15961 tcctctctgt agaagatagg tccattgcgg atgcacttgc caactttgct gaagaacaag
    16021 gaaaaatcgc agaggagtcg tcaaatgagc tcatctaaaa caatcggaat tatcggtggc
    16081 ggtcaactgg gtcagatgat ggccatttct gctatctaca tgggccacaa ggttatcgcg
    16141 ctggatcctg cggcggattg cccggcctct cgcgtggcgg aaatcattgt ggcaccttat
    16201 aacgatgtgg acgccctccg tcagttggca gaccgttgcg atgtcctcac ttacgagttt
    16261 gaaaatgtcg acgctgacgg tttggatgcc gttatcaagg atggacaact ccctcaagga
    16321 acagatctgc tccgcatttc gcaaaatcgt atttttgaaa aggacttttt gtcaaacaag
    16381 gctcaagtca ctgtggcacc ctacaaggtc gtgacttcta gcctagactt ggcagatatc
    16441 gacttgtcga aaaactatgt cctcaagact gcgactggtg gctacgatgg tcatggacaa
    16501 aaggttattc gttcagaagc agacttggaa gcagcctatg cgctagcaga ctcagcagac
    16561 tgcgtcttgg aagaatttgt caactttgac cttgagattt ctgtcatcgt gtcaggaaat
    16621 ggcaaggagg tgacgttttt cccagttcag gaaaatatcc accgcaacaa tatcctgtct
    16681 aagaccatcg taccagcccg catttctgaa agtctagcag acaaggctaa agctatggca
    16741 gtgcgaatcg cagaacaact caacttgtct ggaactctct gtgtggaaat gtttgcgact
    16801 gatgatgacg ttattgtcaa tgaaatcgcc ccacgaccac ataactctgg gcactattct
    16861 attgaagcct gtgatttctc tcagtttgac acccatattc tgggtgttct cggagcacca
    16921 ttaccagtca tcaaactcca tgcgccagcc gttatgctta atgtcctcgg tcagcatgtc
    16981 gaggctgctg agcaatatgt gacagaaaat tcaagcgccc acctccacat gtatggtaaa
    17041 atagaagcga agcacaaccg caagatggga catgtgacct tgtttagtga tgtaccggat
    17101 agtgtggatg agcttgggga agggattgat ttttaggaca agtctatgat acaaattatc
    17161 gttaatacat ttattgaaaa ggataagact ggagcagttg ttgaagtgtt gtatgccagt
    17221 gctgaccaag ataaggtaca agctaaatat gaagaactag ctgcacaata ccccgaaaat
    17281 tatttagcta tctatgatgt accgctggat acggatttga atacactaga tcattacccg
    17341 tctgtgttta ttgaaaaaga ggagtttgag tagaaatctt ggtttaccta gatagcttat
    17401 tcccaacagc ttaagaagaa aggaaaaatt aacacatgat caaccgttac tctcgccctg
    17461 agatggcgaa tatttggagt gaagaaaata aataccgtgc ttggcttgag gtggaaatcc
    17521 tctctgacga ggcatgggct gagttggggg aaatccctaa ggaagatgtg gctttgattc
    17581 gcaagaaggc ggactttgac atcgaccgta ttttggaaat tgagcaggag acgcgccacg
    17641 atgtggtggc tttcacgcgt gcggtttctg agactcttgg tgaagagcgc aagtgggttc
    17701 actatgggtt aacttctacc gacgtggtgg atactgctta tggttacctc tacaagcagg
    17761 ccaacgacat catccgtcgt gaccttgaaa acttcactaa tatcatcgct gacaaggcca
    17821 aggagcacaa gttcaccatc atgatggggc gtactcatgg tgtgcacgct gagccgacaa
    17881 cctttggtct taaattggca acttggtaca gcgaaatgaa acgcaatatc gagcgtttcg
    17941 agcatgcagc tgctggtgta gaagctggta agatttctgg tgcggttggg aactttgcca
    18001 atataccacc atttgtagag gagtatgtct gcgataaact tggcatccgt gcccaagaaa
    18061 tctctacaca agtccttcct cgtgaccttc acgctgagta ctttgcggtt cttgccagca
    18121 ttgcgacttc aatcgaacgt atggcgactg agattcgtgg tctacaaaaa tctgagcaac
    18181 gcgaagtaga agagttcttt gctaaagggc aaaaagggtc ttcagcaatg cctcacaaac
    18241 gcaacccaat cggttctgaa aatatgactg gtctggcgcg tgtcattcgt ggtcacatga
    18301 ttacggctta tgaaaacgtc gctctctggc acgaacgcga tatttctcac tcatcagctg
    18361 agcgtatcat cacaccagat acgaccattt tgattgacta catgctcaac cgttttggaa
    18421 atatcgtcaa gaacttgaca gtcttcccag aaaatatgat ccgaaacatg aactcgactt
    18481 ttggtcttat ctttagccaa cgggctatgt tgacattgat tgaaaaaggc atgacccgtg
    18541 agcaagccta tgacttggtg caaccaaaaa cagcctactc ttgggacaac caagtagact
    18601 ttaaaccact tcttgaggca gattcagaag taacatcacg tctcactcaa gaagaaatcg
    18661 atgaaatctt caacccagtt tattacacca aacgagtgga tgatatcttt gaacgtcttg
    18721 gactaggtga ttaattaaaa aataaacagc gagcttcaat ctcgctgttt attttttatc
    18781 gaaaagactt agtcttcttt tcttttagtg agtccatagg ctgctagtgt ggacatgagt
    18841 cctgcgacta ctagtcctgc agaatcgtga gttcctgttt caggaagttt tttctctgtt
    18901 accacaggag ctggatcttg aggaagagct ttgctttcct cagcaggagc agttgatgga
    18961 gctggttgtc ttgggatttc tagttttggt ttttcttcag caatagcggc ttgtccgttt
    19021 tcatcgccta catgtgttac catagttccg acttcgacta tttgagtaac ggcttcctgt
    19081 gctacgacac tatttacaag tgttttcact tccttaccat cggcagaagt gctcacagag
    19141 tagaagttgc tacgatgtcc attgacgccc ttagtaatga cttgtgtttt tcctttgagt
    19201 aagagtggat tttcacgagt cactgtggta aatggaattt cttctttttg gatatccagt
    19261 ctaggtttta cctcagtagt tggtgcaaga ccactttcat cacccttgtg agttacagga
    19321 gcgccaactt caaccacttg gtttataact tctttggtta cctggctatc aaggactgtt
    19381 tctgttgttt ttccattttc agtgagtaca gagatgtaat gagttcgttc acctttgact
    19441 cctgctgtga taatattttc ctgaccagct gggaggttag gattttcttt cttgataact
    19501 tcaaatggaa tttcttcagt tcttgtgatg agttctggtc tggtttcaac attggcagcc
    19561 acttcatttt catctaggct tcctgaatga gttgcagctg gtttgaggcc ttgaagagcg
    19621 gcttttaggt tggctacaag tgtgtcaagc tcagcttgtt tattacggtt gaggttgtaa
    19681 tttagagctg ttttagctgc gtcaagggcc tcaagacttt ctttactata tccttctaag
    19741 tttgtaggaa ttttagctaa ttcttcgcgg agagcattat aattagcacg gaagtagtct
    19801 ttgttgtggt ctgcaaaggc agtcatgagt tcaaagattt cctcttcctt gtattcagcg
    19861 cttggtctat ctgcccagat tgaaagcata cttccgactg ttggaagatc tacttcagga
    19921 tatttggtag aagctagttg attgaatggt gtttttccag tattctcaat agctttcttg
    19981 aggaaaccac caccatcttc tggtttttga ccaagaatgt agtaccagtc accgttggta
    20041 ttcaagaatt tatagccttt gcttgctagg tattgaggtg atgcgaggtt atatccccac
    20101 cagcctttag accagtaaga aatcaagaca tctttgtcaa actgaacatc gtccttgtct
    20161 tcatagtaga agccatcgtt gaaggccatt ggttgaagcc ctctttcttt ggccatagct
    20221 gcgagggtgt tggcatattc ggcaaatttg ccatagagtt gataccactt gaggtagtac
    20281 cagccttggg cactagtcgc atcgttggcg tattcgtcag taccaaagtt gaaaatcttt
    20341 gttttacctg caaagaagtc catgtattta ccgatgaggg cttttacaaa gttcatcgct
    20401 tcttcgtttt tcaagtccat agttgttttt gaaactttat caaagtgggc ttgaggattt
    20461 ttaataccta atttttccat ggcaaccagc atagcatcca tgtgacctgg actgttaata
    20521 gctgggatga gaccaatgtc cttagattta gcgtattcaa ttagctctgt cacttctgcc
    20581 tgtgttagtg cagtaccgtt tggatcgtcg tagtaagctt tagttccttc gataatagct
    20641 tttttaacgt catcactagc ataggttttt ccgttggcag taatggtcat atcattgagt
    20701 agaaagcgaa gtccgtcatt tcctagaagg agatggacat cagaatatcc gagctcactg
    20761 gccttgtcta cgatgcgttt gagctggtcc agagtaaagt atttgcgtcc agcatcgatt
    20821 gagattacct tgtttttggc aagtttttca acctcacgtt tagcttcttc ttctttttga
    20881 gcttcaggcg tgagggtcaa gttgttgaca gtttcttgaa gtttagcaat ggcttgatca
    20941 atcgtatctt gttgggcacg gctaaggttg ctatcgagag agcgaatagc tttttcagct
    21001 tcttttacgg ccgcgacgct ttctgcagta taacggttca ggtcttttgg tacctcgtta
    21061 agtgcttgct ctgcagattc ataatcagct gcgaagtatt cagcgttggc atttgcaaaa
    21121 tgacgcatga gtttgaagag gtgtgatggt gaataacgtg cagatggagt gtcagcccaa
    21181 gcagctacca taccaccgat gattgggata tcagctcctt ctgtttttgg tacagaagtg
    21241 attggtgtgt ttttaatacc attgagcccc tgatcgagat tgtaccagcc ttggccatca
    21301 gcgtttcgtc caagaacgta gtaccaagca tcattggtat taaggatttg gtgacctttt
    21361 tcagctagta gtttagaaga agcgacatcg tagcctcccc aaccaccagt ccacatagaa
    21421 acgatgatgt ctttgtcaaa actaccaaag cttgtgtcgc tattgtagta gataccgtcg
    21481 ttaaaggcca ttggtttgag accgtgcgat tttacaatac gagcgaggtc attggcgtag
    21541 gcaataaatt tttcatagcc ttttacaggg tagccttcgt ttggatagta tttatcagct
    21601 tgaagcacac tccaaccttt agcatctgtc gcatcattgg catattcatc aagtccgatg
    21661 ttgaagattt cagtcttttt cgcgaaataa gcagcatact tgtcgataag ggcttttgta
    21721 aaagcgacag cttgttcgtt gtcaagatcg acagtacggg ctgattcctt cccaaaatag
    21781 ctaaagttag ggttttggat tcccaattct ttcatggcat tgagaatcgc atccatgtga
    21841 ccaggactat ttactgtcgg aatgagaccg atacctttat cttttgcata gttaatcaga
    21901 tctgtcattt gactttctgt taagtgattg ccgtttggat cgttgtaata atcatttgta
    21961 cctttttcaa tggcgcgttt gacatcgtca ctggcatagg tcttgccgtt agctgtgatg
    22021 ctcatatcgt ccaacatgaa acggagtcca tcatttccga ctaataggtg taaatcagtg
    22081 tagccataat gtttcgcttt atcgatgatt tccttgagct gttctggtga gaaatattta
    22141 cgtccagcat caatagaaac aattttcttt ttcgctagtt tttcacttac agttgcagca
    22201 cgttcctttc ctgcctctgt tgccggtttg tcagcctctg ctttcgcttc atctttttta
    22261 gctggtttat cctttttagg gctagcttct tctgcctttt tattagcagt ttctttttca
    22321 gcagaagttg gagttaccac ttctgcttta tcactaggag ttgaactaac ttcctcttgt
    22381 ggtttttctt ctgtttttgg aagactagct accttatcag tagctggagt ttctgtttct
    22441 acagtttttg gagcttctgg ttgaagctct gctttaggtg tttcctcagt ccgattttcg
    22501 gatgattgag gggaatcaga aaccgtatgg atggtcggtt ggttttctgt agtagtagga
    22561 gtaactccat cggctgcaac agtctgtgct tggaaggcaa atccaattag aacagaagct
    22621 gctcctacag cgtatttacg aatagaaaaa cgctgttgtt tttcatgttt cattgcaaaa
    22681 cctcctgatt gcattgttat attgatagcg attatataaa tcaacgcctt tattttattt
    22741 cttgttttaa cgagagtcaa gaggagatga caaaaaacta taataagtat aaaaaaatat
    22801 aaaatttaaa cttaagattt cagattggtc gaaaaaatac atatatatat ctagtataat
    22861 ttttggttct atttctataa aatattccac aaattataga atcttccaaa aataggtaag
    22921 cgctaccttt ttggtgtggt ataataagca tagaaaaagc ccgagcgatt aggctcaggc
    22981 tttcttctta gtgatcacgg tcacatgaga tgaatttaat cttgtagtaa tcagatcgtt
    23041 tgtaagtttc actgtattct aaaacttggc cagttgattc gagtttggtg attttagttt
    23101 gtaggacagt agggaattgt tcatcgactc cgaggactga agccgcatgt tctggagttg
    23161 gaaagactat ttcgttgatt tcttcaaagt gttcatcatt catgtgaatg tggtagtcta
    23221 acttgaaacg attatagata gaactatagt attcaaggtt tggataattt gcgttgatat
    23281 attgttctgg gatgtaggat gtatggtaga tataaacgac accgtttgat tcgcggatac
    23341 gttcaatctt gtagtagaat tgatcgccgc gtagacccaa tttttccaag taaacaagct
    23401 tgtttccgcg ttcaattgaa agaacagtta ccttatcatc tttagcattg aagagttcaa
    23461 tatctgaaaa ctctacaagc ttgtgtttgc gtgcacgtga aacgaaggtt ccttttcctt
    23521 gttggcggac aatatagcca tctttggcaa ggtcgtttaa ggcgcgaaca actgtgatag
    23581 agctgacatc gtacattgaa atgagttctg cttcagtgta aaatttatct ccactgctaa
    23641 actgcccaga gatgatttta ttttttaatt cgtcttttat gtattgatat ttgggaattg
    23701 ccatattttt acctctattt tccttctcca tagaaaattt taccataaaa gcgaaaaaaa
    23761 tagtagtctt ttgaataaaa aattagaata atagtttgaa aaattaagcg atatagggat
    23821 tgctcatatt ttatcaatgt attctataca aaattatttt agatcctata ttccgaattt
    23881 aaagagcgtt tgaaatgggt ctaaaaatgt ttgaaactaa ttgaatgtgt gtataatagt
    23941 aaaaatgcag taaatatccc gacaatttct attgacaatt caaacagatt ggtttatgat
    24001 taatataaca acaaatgaaa tcgcaaactt tcgcggtcgg aaggtagttt tatgacacga
    24061 tttgagatac gagatgattt ctatctcgat ggaaaatcat ttaagatttt atctggtgcc
    24121 attcattatt ttagggttcc tccagaggat tggtatcatt cgctctataa cttgaaggct
    24181 cttggtttta atacggtaga gacttatgtt gcttggaatt tacacgagcc tcgtgaaggt
    24241 gagtttcatt ttgaaggtga tctggattta gagaaatttc tccaaatagc gcaggatttg
    24301 ggtctctacg caattgtgcg tccgtctcca tttatctgtg cggaatggga attcggtggc
    24361 ttaccagctt ggctcttgac caagaacatg cgaattcgct catccgaccc agcatatatc
    24421 gaggcagttg gtcgctacta tgatcagtta ttgccaagac tggtgcctcg tttgttggac
    24481 aatggtggca atattctcat gatgcaggtt gaaaatgagt atggttctta cggagaagat
    24541 aaggcttacc tgagagcgat tcgacagcta atggaagagt gtggcgtaac ctgtcccctc
    24601 tttacatcag atggtccatg gcgagctact ctgaaagctg gaaccttaat tgaagaggac
    24661 ctctttgtaa caggaaactt tggttctaag gcaccttaca acttttcgca gatgcaggaa
    24721 ttctttgatg aacatggtaa gaaatggcca ctcatgtgta tggagttctg ggatggttgg
    24781 ttcaatcgct ggaaagaacc gattatcaca cgggatccta aggaattggc agatgcagtt
    24841 cgagaggttt tggaacaagg ctctatcaat ctttacatgt tccacggtgg tacaaacttt
    24901 ggtttcatga atggttgctc agctcgagga actttggacc tgccacaagt tacgtcttat
    24961 gattacgatg cccttctgga tgaagaagga aatccaactg ctaaatatct tgcagtcaag
    25021 aagatgatgg caacacattt ttcagagtat ccgcagttgg aaccactcta caaagagagt
    25081 atggagttgg atgctattcc actagttgaa aaagtttctt tgtttgaaac cttagatagc
    25141 ttgtcaagtc ctgtagaaag tctctatcct caaaagatgg aggagctggg acaaagttat
    25201 ggctacctac tttatcgaac agaaacaaac tgggatgcag aagaagaaag acttcgtatc
    25261 attgatggtc gagatagggc ccagctgtat gtcgatggtc agtgggttaa aactcaatat
    25321 cagacagaga ttggggaaga tattttttat caaggtaaaa agaaatggct atctaggtta
    25381 gatatcttga tagaaaatat ggggcgtgtc aactatgggc ataagttctt agcggatacg
    25441 caacgtaagg gaattcggac aggggtctgt aaggatctgc atttcttact aaactggaaa
    25501 cactatccac tcccactaga caatcctgag aaaattgatt tttcaaaagg atggactcaa
    25561 ggacaaccag ccttttacgc ttatgacttt acagtcgaag agccaaaaga tacttaccta
    25621 gacttgtctg agtttggtaa gggggttgcc tttgtcaatg ggcagaatct aggacgtttt
    25681 tggaacgttg gcccaactct ctcactttat atccctcata gctatctcaa ggaaggtgcc
    25741 aaccgcatca ttatctttga aacagaaggt caatataaag aagagattca tttaactcgt
    25801 aaacctacac taaaacatat aaagggggaa aacttatgac aattgtagga tgccgtattg
    25861 atggacgttt gatccacgga caagtagcca atctttgggc tggaaaacta aatgtttcac
    25921 gcattatggt tgtagacgac gaagttgtca acaacgatat tgaaaagagt ggtttgaaac
    25981 ttgcgacacc accaggtgtg aaattgagta ttttgccagt tgagaaagct gcagccaata
    26041 ttcttgctgg caaatacgat agccaacgtc tctttatcgt ggctcgtaaa ccagaccgct
    26101 tccttggttt ggtagaagca ggtgtaccac ttgaaaccct taatgttggg aatatgtctc
    26161 aaacaccaga aactcgttct attacacgtt ctatcaacgt agtagacaag gatgtggaag
    26221 acttccacaa actggcagaa aaaggtgtta aacttactgc tcagatggtt ccaaatgatc
    26281 caatttcaga ctttttgagc ttattaaaat aggaaaaaat tttttaggag gtcattgtta
    26341 tgatacaatg gtggcaaatt ttacttctca ctttgtactc agcttatcaa atctgtgatg
    26401 agttgacgat cgtttcatct gcaggttccc ctgtatttgc tggtttcatt actggtttaa
    26461 tcatgggaga tgtgactact ggtttactta tcggtggtaa cttgcaactg ttcgttcttg
    26521 gggttggtac cttcggtggt gcttctcgta tcgacgcaac ttctggtgcg gttcttgcga
    26581 cagccttctc tgtttcacaa ggaattgatg caccgcttgc aattactaca atcgctgtac
    26641 cagtagcagc tctcttgact tacttcgacg ttcttggtcg tatgactact accttcttcg
    26701 ctcaccgtgt ggatgctgca atcgaacgct ttgactataa aggtattgaa cgcaactact
    26761 tgcttggtgc gattccgtgg gctctatctc gtgcccttcc agtcttcttt gcccttgctt
    26821 ttggtggtgc ctttgtacaa tcagtagtag acttcgttga agcctacaaa tgggttgcag
    26881 atggcttgac acttgcagga cgtatgcttc caggtcttgg atttgcaatc ttgcttcgtt
    26941 accttccagt taaacgtaac cttcactacc ttgctatggg atttggtttg acagctatgt
    27001 tgactgttct ttactcatat gtaacaggtc ttggtggcgc tgttgctggt atcgtaggta
    27061 ctcttcctgc tgaagttgct gaaaaaattg gtttcgtgaa caacttcaaa ggtttgtcta
    27121 tgattggtat ttctatcgta ggtattttcc ttgcagtgct tcacttcaaa aatagccaaa
    27181 aagtagctgt agcagcacct tctacaccat cagaaagtgg ggaaatcgaa gatgacgaat
    27241 tctaattaca aacttacaaa agaagatttt aatcaaatca acaaacgtag cttgtttact
    27301 ttccaattag gttggaacta cgaacgtatg caagcttctg gttaccttta catgatcttg
    27361 cctcagttgc gtaaaatgta tggtgatgga actcctgaat tgaaagaaat gatgaaagtt
    27421 catactcaat tcttcaatac ttcaccattc ttccatacca ttatcgctgg ttttgacctt
    27481 gccatggaag aaaaagatgg tgtaggttca aaagacgctg ttaacggtat caagacaggt
    27541 ttgatgggac cattcgctcc tcttggggat acaatctttg gttcacttgt acctgctatc
    27601 atggggtcag tcgcagcaac tatggctatc gctggccaac cttgggggat cttcctttgg
    27661 attgcagttg cagtagcgta tgacatcttc cgttggaaac agttggaatt tgcttacaaa
    27721 gaaggggtta accttatcaa caacatgcaa agtaccttga cagctttgat tgacgctgca
    27781 tctgtacttg gtgtcttcat gatgggtgct cttgtagcaa cagtgattaa ctttgaaatt
    27841 tcttacaagt tgccaatcgg tgaaaagatg attgatttcc aagacatctt gaaccaaatc
    27901 ttcccacgtt tgcttccagc aatctttact gcctttatct tctggttgct tggtaaaaaa
    27961 ggtatgaact ctactaaagc tatcggtatt attatcgtac ttgctttggc tctttctgcc
    28021 cttggtcact ttgcacttgg aatgtaattc cttatgacta aatcattaat tttggtgagc
    28081 catggtcgct tctgtgagga gcttagaggt agcacagaaa tgattatggg cccacaagac
    28141 aacatttaca cagtagctct tcttccagaa gatggcccag aagaatttac tgctaaattt
    28201 gaagctgtta ttgaaggatt ggatgatttc ctagtctttg cggatcttct cggtgggaca
    28261 ccttgtaatg tggtgagtcg cttgatcatg gaaggtcgtg atattgacct ttacgcaggg
    28321 atgaatcttc caatggtgat tgaatttatc aatgcgagcc ttacaggcgc agatgcggac
    28381 tacaagagcc gtgctgcaga aagcattgtg aaagttaatg acctgttagc gggcttcgat
    28441 gatgacgaag atgaataata ctcttcgaaa atctcttcaa actacgtcaa cgtcgccttg
    28501 ccgtaggtat atgttactga cttcgtcagt cttatccggc aacctcaaaa cggtgttttg
    28561 agctgacttc gtcagtctta tccggcaacc tcaaagcagt gctttgagca gcctgcggct
    28621 agtttcctac agattttagt tggaactcga ttcaattcat gtgacaacgt gaaaatcgtt
    28681 agagcatttt atatagaata tacatgggaa tggagcttac tcccattccc atatttaata
    28741 gaaaaagagg aactcaatgc tacattatac aaaagaagac ttgctcgaat tgggtgcaga
    28801 aatcactacg cgtgaaatct accaacagcc tgatgtatgg agagaagctt ttgaatttta
    28861 tcaagcaaaa cgtgaagaaa ttgcagcctt tctacaagaa atcgctgata aacatgacta
    28921 tattaaggtt atcttgacag gtgctgggac ttctgcttat gtgggagata ccttgctacc
    28981 ttactttaag gaagtctatg acgaacgcaa atggaatttc aatgctattg cgacaacaga
    29041 tatcgttgcc aaaccagcaa cctatttgaa aaaagatgtg gcaactgtcc ttgtgtcttt
    29101 tgctcgtagt gggaactcgc ctgaaagtgt ggcgactgtt gatttggcca aatccttggt
    29161 ggatgagctt tatcaagtga cgattacttg tgcagcagat ggtaaattgg ctcttcaagc
    29221 tcacggtgat gaccgtaatc tcttgctctt gcaaccagct gtctctaatg atgctggatt
    29281 tgccatgact tctagcttta cgtctatgat gttgacagct ctcttggtct ttgatcctac
    29341 agaatttgct gttaagtctg aacgttttga agttgtatct agtcttgccc gtaaagtttt
    29401 agacaaggta gaagatgtca aagagctcgt tgatttagac tttaaccgtg tcatctatct
    29461 aggcgctggt cctttctttg gacttgctca tgaagctcag ctcaagattt tggaattaac
    29521 tgctggtcaa gttgcgacca tgtatgaaag cccagttggc ttccgtcacg gtccaaaatc
    29581 tcttatcaac gacaatacag ttgttttggt ctttggtaca acgacagcct acactcgtaa
    29641 gtacgacttg gacttggttc gtgaagttgc tggtgaccag attgctcgtc gtgttgtgct
    29701 tttgagtgat caagcttttg gtcttgaaaa tgtcaaagaa gtggcccttg gttgtggcgg
    29761 tgtcttgaat gatatttacc gtgtcttccc ttacatcgtt tatgcccaac tctttgccct
    29821 attgacttca ctcaaggtag aaaataaacc agatacaccg tctcctacag gtacagtaaa
    29881 ccgtgtagta caaggtgtca taattcacga atatcaaaag taagacagtg tttatgaatt
    29941 cttgacaaga ggatttgtaa attatcagat aaaccataga ttgtcagtac gctttctatg
    30001 gtttgtttgc ttgagagaaa tagtaaaagg agaacagaat gaaagcatac acagagcgtg
    30061 tatttggaaa tgttgagggc gaggatgtct tggcctatcg atttgagaca gacggtggct
    30121 accaacttga ggttatgact tatggcgcga ccatcttgcg ctatgtcgca cctgacaagg
    30181 ctggaaattt tgccaatgtt atcttgggat ttgatgactt tgatagttat gtaggcaata
    30241 gtcccaagca tggagcaagt gtaggtcctg tagcgggtcg tattgcaggt gcgacctttg
    30301 agctcaatgg taagacctat gaccttgagg ttaataatgc tagcaactgt aatcacagtg
    30361 gttcaactgg ttgggattcc agcttgtttg aagttgaaga agtaagcgat catggcttga
    30421 ctctctacac agagcgtaca gatgggacag gagggttccc tggaaatctc aagatttgga
    30481 tcagttatca cttggaagaa actggtgcct atgaaatcat ctacaaggta acgaccgatc
    30541 aggatacgct ggtcaatcca accaaccaca gctatttcaa cttgtctggt gatttcacgc
    30601 agacgattga ccgtcatgtc ttccaactaa acacagaggg catttactca atcgctcctg
    30661 acggtgttcc tgccaaaact ccagaagcca accgtgatgt ggtcaaacac atctacaatg
    30721 gtaccttgtt gaaggatatc tttgcagaag aagatgagca aatccagctg gcatcaggtt
    30781 tggatcaccc atttgccctt cctgcaggcc atgacaatgc tggattcctt tatgaccaaa
    30841 attcaggtcg cttcctgctt ttcaagacag aagctccttg ctttgtggtc tacacagcaa
    30901 actttgtgga tgaaagtgtc atcataggag gtcagccaat gctacagcac aatgggattg
    30961 ctcttgaagc gcaagcttta ccagatgcca ttcacagtga ccttaaaggc caagtcattc
    31021 ttaaagctgg tcaaaccttc accagtaaga cacgttatga acttgttgtg aaataaaaaa
    31081 agctggtcaa aaagtcttta aaattaaaat aacgcatatt gtcaggtgat gaaaagtttg
    31141 ataatatgcg ttttattatg aaatgattta ctttatctta tcctgaaatg gtgttatttt
    31201 tgagttatct ggcttgactt tcttgatgga aattatataa tagttgtaat aacaataatt
    31261 acaacgagaa aagaggacca tttatgacct atgaatacaa gagtcacatt tatttggcag
    31321 agacagtttt aaatgtaaag gatttggcca gtcaaacagc cttttatcag caagttattg
    31381 gtttagaaat cttatctcaa actgagacag agtccattct aggccttggt ggaaaagtct
    31441 tggtacagct gattcaagca caagagagcg gagaagtgag ggaacattat ggtctttacc
    31501 atttggccat tctcttgccc acacgcaagg ctttggctga tgtcttgaaa cacctgacag
    31561 atttgcaaat tcctcttgtc ggcggtgcag accatggtta cagtgaagct atttacttgg
    31621 aagatttgga gggaaatggc attgaactct atcgagataa gccagtttcc acatggaata
    31681 ttcgagaaga tggacgcatt atcggagtga cggaggctct tgcggcgcag gacatttatg
    31741 agttggggga aagagtagag ccctttatcc tggcagaagg tacaagaatg gggcatattc
    31801 atctttccgt caaggatagt cgaaagtcca gccagtttta tcaaaaggtg ttagggctag
    31861 agaataaatt cagtgtgcct ggcgctagtt ggattgcggc tggagattac catcatcatt
    31921 tagcagttaa cgaatgggga ggaaaaggtc tggcttcgcg taagcaaggc ttaccaggtt
    31981 tagcctacta tgtcatcgaa gtcgcacaca aaaaagaact attaacgatt gcccaacgag
    32041 cacaagaagt tgacgcacca atcaaatgga tgacatcgag ccaattggaa atcacagact
    32101 cagatggcat cgtgacccgt attcgtttag caaggtaaaa ttaggaattt ttaataagtt
    32161 ataagtattg ctagtttgct ttttgaacgg tctttggtaa cagaactgcg tgagctggat
    32221 gggtgagaat tttgacaaag aagctgatta gagatataat gaagaaaaat cgagcaagga
    32281 aaaagaaatg acaaattcaa agtatattac ttgtttgaaa cgttcagagg gccagttgtg
    32341 tgggattcaa aagatgattg aaggagattg tgactgtgct gatattgtga cgcaactgac
    32401 agcagtgaga tctagtgtag agcgcgtgat tgagatgata attaccgaaa atcttactga
    32461 atgtatcaat cagcccctag atgattctga agctcaaaag gaacgcctag aaaaggctat
    32521 ccgatacttg attaaacgga aataaatgaa tgttttgaga cgatatgtgc aaaggtctat
    32581 taactattag aatagtttaa tgtgagtcaa aagaagttgt aaaaaatctt taaaattaaa
    32641 aaaacgcata gtatcaggtg ttgaatgtac tgaccccaaa aagttagatt ttttctgtct
    32701 aacttttggg gtgcagttca taagaacctt ggtaatatgc gttttattat gtagagactt
    32761 atactcttcg aaaatctctt caaaccacgt cagtttcacc ttgccgtaga tatggttact
    32821 gacttcgtca gttctatcta caacctcaaa acagtgtttt gagctgactt cgtcagttcc
    32881 atccacaacc ttaaaacagt gttttgagca gcctgcggct aacttcctag tttgcacttt
    32941 aattttcatt gagtattact tcatcttctc ctgaaattga gtttttgccc agctcctctt
    33001 gattattcta cttgaccggg ccaagcattc ataccacctt ctacgttggt aacggtgagg
    33061 ccttgggagc tgagaaattg gcaggtagat gcagagcgga ctcctccttg acaaataaca
    33121 tagtattcat ggtcaggttt gagttctttg tagccttgct ctaaggtact taatggaaga
    33181 tttttggcac ctggtgcatg tcctgcttgg aattcatgtg cttcacggac atcgataagt
    33241 tctagatttt cattttggta ttttccataa aagtcagcca tgctgatact agtttccata
    33301 ttctactcct tctgggttaa ccattttgta tagtgaataa gcgccgtcaa ggttttgggc
    33361 ggtaaatcct gcttgcttga ggataggctc tgcgatatag ctgcgcaaac cactgtggca
    33421 gctgacgatg taggcttgtt ttttgtccag ttcatcagct tcctccttga cactttgtca
    33481 gtttttgata caatagtaca aaattagagg aggcaggcta tgattcagaa acatgcgatt
    33541 cctattttag agtttgatga caatcctcag gcggttatca tgcccaatca cgaggggttg
    33601 gacttgcagt tgccaaagaa gtgtgtctat gcttttttag gtgaggagat tgaccgctat
    33661 gcgagggaag taggggcgaa ctgtgttggc gaattcgttt ctgctaccaa gacctatcca
    33721 gtctatgtca tcaactacaa gggtgaggag gtctgtctgg ctcaggctcc tgttggctcc
    33781 gctccagcag cccagtttat ggattggttg attggctatg gtgtggagca gattatctct
    33841 actgggacct gtggtgtcct agctgatata gaggaaaatg cctttctagt ccctgttcgc
    33901 gctttgcgag atgagggagc cagttaccac tatgtggcac cttgtcgtta tatggaaatg
    33961 cagccagagg ctattgctgc tattgaggaa gttttggaag acagagggat tccttatgaa
    34021 gaagtcatga cctggacgac agacggtttt taccgagaaa cggctgaaaa ggtggcttat
    34081 cgtaaggaag aaggctgtgc tgttgtggag atggagtgtt ctgctcttgc ggcagtagct
    34141 caattgcgtg gggttctctg gggtgaattg ttgttcacag cagattctct agcggacttg
    34201 gaccagtacg acagtcgtga ctggggctcg gaagctttta ataaggcgct agaactgagt
    34261 ttagcaagtg ttcaccacct ttagttgtac aggcaaagga tttgttttat cataaaatgt
    34321 ctagatcata cttttcaaaa atatgtttaa acgaggtcac cttcctcttg tcctaggcat
    34381 gttgaggttg ggaaaaatct ttaaaatcag aaaaacgtat catatcaggt gatgaaaatt
    34441 ttgatactat gcgttttatg ttggtaagat ttagagtgag atgaaatgat actcttcgaa
    34501 aatctcttca aaccaggtca gcttcacctt gccgtataga tgttactgac ttcgtcagtt
    34561 ctatctgcaa cctcaaaaca gtgttttgag ctgacttcgt cagttctatc cacaacctca
    34621 aaacagtgtt ttgagcaacc tgtgactagc tttctaagcg atgccttggt tctcattgcc
    34681 tataatcaaa aagagaaatt ttctcctgaa aagcatatag agtagctggc gttaaaagct
    34741 cctgtcttgc ttttttgatc tatagtcaca tctatcaagt attgttcttg cctaagctat
    34801 caataaaaag gtggcatttt ttaggcttgg tgttagtgaa ttttgcctta tcctatctaa
    34861 gtcatttcga gctttttatg gtacaatgga aacatgttat tcaaattatc taaggaaaaa
    34921 atagagctag gcttatctcg tttatcgcca gcccgtcgta tttttttgag ttttgccttg
    34981 gtcattttac taggctctct tcttttgagc ttgccctttg tccaagttga aagctcacga
    35041 gcgacttatt ttgatcatct tttcactgct gtctctgcag tctgtgtgac aggtctctca
    35101 acccttccag tagctcacac ctataatatc tggggccaaa taatctgttt gctcttgatt
    35161 cagatcggtg gtctagggct catgactttt attggggttt tctatatcca gagcaagcag
    35221 aaacttagtc ttcgtagccg tgcaactatt caggatagtt ttagttatgg agaaactcga
    35281 tctttgagaa agtttgtcta ttctattttt ctcacgacct ttttggttga gagcttggga
    35341 gctattttgc ttagttttcg ccttattcct caacttggct ggggacgtgg tctttttagt
    35401 tccatttttc tagcgatctc agccttctgt aatgccggtt ttgataattt agggagcacc
    35461 agtttatttg cttttcagac cgatttactg gtcaatctgg tgattgcagg cttgattatt
    35521 acaggcggcc ttggttttat ggtctggttt gatttggctg gtcatgtagg aagaaagaaa
    35581 aaaggacgtc tgcactttca tacgaagctt gtactattat tgactatagg tttgttgtta
    35641 tttggaacag caactactct ctttcttgag tggaacaatg ctggaacgat tggcaatctc
    35701 cctgttgccg ataaggtttt agttagcttt tttcaaacag tgacgatgcg aacagctggc
    35761 ttttctacga tagattatac tcaggctcat cctgtgactc ttttgattta tatcttacag
    35821 atgtttctag gtggggcacc tggaggaaca gctgggggac tcaagattac gacatttttt
    35881 gtcctcttgg tctttgcacg aagtgagctt ctaggcttgc ctcatgccaa tgttgcgaga
    35941 cgaacgatcg cgccgcgaac ggttcaaaaa tcctttagtg tctttattat ctttttgatg
    36001 agcttcttga taggattgat tctgctaggg ataacagcca aaggcaatcc tccctttatc
    36061 cacctcgtat ttgaaaccat ttcagctctt agtacagttg gtgtaacggc aaatctgact
    36121 cctgaccttg ggaaattggc tctcagtgtt atcatgccac ttatgtttat gggacgaatt
    36181 ggtcccttga ccttgtttgt tagcttggca gattaccatc cagaaaagaa agatatgatt
    36241 cactatatga aagcagatat tagtattggt taagaaagga aagagcatgt cagatcgtac
    36301 gattggaatt ttgggcttgg gaatttttgg gagcagtgtc ctagctgccc tagccaagca
    36361 ggatatgaat attatcgcta ttgatgacca cgcagagcgc atcaatcagt ttgagccagt
    36421 tttggcgcgt ggagtgattg gtgacatcac agatgaagaa ttattgagat cagcagggat
    36481 tgatacctgc gataccgttg tagtcgcgac aggtgaaaat ctggagtcga gtgtgcttgc
    36541 ggttatgcac tgtaagagtt tgggggtacc gactgttatt gctaaggtca aaagtcagac
    36601 cgctaagaaa gtgctagaaa agattggagc tgactcggtt atctcgccag agtatgaaat
    36661 ggggcagtct ctagcacaga ccattctttt ccataatagt gttgatgtct ttcagttgga
    36721 taaaaatgtg tctatcgtgg agatgaaaat tcctcagtct tgggcaggtc aaagtctgag
    36781 taaattagac ctccgtggca aatacaatct gaatattttg ggtttccgag agcaggaaaa
    36841 ttccccattg gatgttgaat ttggaccaga tgacctcttg aaagcagata cctatatttt
    36901 ggcagtcatc aacaaccagt atttggatac cctagtagca ttgaattcgt aaagagggat
    36961 gacccctctt ttttgatgcc taagatggca aatagagaca gaagcccctt gtcttctagt
    37021 aaaagttctt caaaggctgg actttatggt aaaatagaaa gaagtgacaa gagagagtaa
    37081 tactcaatga aaatcaaaga tcaaactagg aaactagcta cgggctgctc aaaacactgt
    37141 tttgaggttg cagatagaac tgacgaagtc agtaacatct atacggcaag gcgacgttga
    37201 cgcggtttga agagattttc gaagagtata agaaaaaatc agtcccctaa aggagtagat
    37261 tatgaagtta ttgtctatcg caatttctag ctataatgca gcagcctatc ttcattactg
    37321 tgtggagtcg ctagtgattg gtggtgagca agttgggatt ttgattatca atgacgggtc
    37381 tcaggatcag actcaggaaa tcgctgagtg tttagctagc aagtatccta atatcgttag
    37441 agccatctat caggaaaata aatgccatgg cggtgcggtc aatcgtggct tggcagaggc
    37501 ttctgggcgc tattttaaag tagttgacag tgatgactgg gtggatcctc gtgcctactt
    37561 gaaaattctt gaaaccttgc aggaacttga gagcaaaggt caagaggtgg atgtctttgt
    37621 gaccaatttt gtctatgaaa aggaagggca gtctcgtaag aagagtatga gttacgattc
    37681 agtcttgcct gttcggcaga tttttggctg ggaccaggtc ggaaatttct ccaaaggcca
    37741 gtataccatg atgcactcgc tgatttatcg gacagatttg ttgcgtgcta gccagttcta
    37801 actgcctgaa catacttttt atgtcgataa tctctttgtc tttacgcccc ttcagcaggt
    37861 caagaccatg tactatctgc ctgtcgattt ctatcgttat ttgattgggc gtgaggacca
    37921 gtctgtcaat gagcaagtga tgattaagtg cattgaccag caactcaagg tcaatcgact
    37981 cttgatagac caacttgatt tgtcccaagt gagtcatccc aaaatgcgag aatatctgct
    38041 gaatcatatt gaactcacga cggtgatttc cagtaccctg ctcaaccgat ctggaacagc
    38101 ggagcatctg gcaaaaaaac gccaattgtg gacctatatt cagcagaaaa atccagaagt
    38161 ctttcaggct attcgtaaga ccatgttgag ccgtttgacc aaacattctg tcttgccaga
    38221 tcgcaaactg tccaatgtcg tctatcaaat caccaaatct gtttatggat ttaattaata
    38281 taagtgtttt ataagaggga tttaagaaaa attttaactt tttcttagtc ctttttaatt
    38341 tcaggagatt atactagagt catcaaataa agaaagactc taaggagaat cctatgaaat
    38401 tcaatccaaa tcaaagatat actcgttggt ctattcgccg tctcagtgtc ggtgttgcct
    38461 cagttgttgt ggctagtggc ttctttgtcc tagttggtca gccaagttct gtacgtgccg
    38521 atgtggtcaa tccgacccca ggtcaagtct tacctgaaga gacatcggga acgaaagagg
    38581 gtgacttatc agaaaaacca ggagacaccg ttctcactca agcgaaactt gagggcgtta
    38641 ctggaaatac gaattcactt ccgacaccta cagaaagaac tgaagtgagc gaggaaacaa
    38701 actcttctag tctggataca ctttttgaaa aagatgaaga agctcaaaaa aatccagagc
    38761 taacagatgt cttaaaagaa actgtagata cagctgatgt ggatgggaca caagcaagtc
    38821 cagcagaaac tactcctgaa caagtaaaag gtggagtgaa agaaaataca aaagacagca
    38881 tcgatgttcc tgctgcttat cttgaaaaag ctgaagggaa aggtcctttc actgccggtg
    38941 taaaccaagt aattccttat gaactattcg ctggtgatgg tatgttaact cgtctattac
    39001 taaaagcttc ggataatgct ccttggtctg acaatggtac tgctaaaaat cctgctttac
    39061 ctcctcttga aggattaaca aaagggaaat acttctatga agtagactta aatggcaata
    39121 ctgttggtaa acaaggtcaa gctttaattg atcaacttcg cgctaatggt actcaaactt
    39181 ataaagctac tgttaaagtt tacggaaata aagacggtaa agctgacttg actaatctag
    39241 ttgctactaa aaatgtagac atcaacatca atggattagt tgctaaagaa actgtcgaaa
    39301 aagctgttaa agataacgtt aaagacagta tcgatgttcc agcagcctac ctagaaaaag
    39361 ccaagggtga aggtccattc acagcaggtg tcaaccatgt gattccatac gaactcttcg
    39421 caggtgatgg tatgttgact cgtctcttgc tcaaggcatc tgacaaggca ccatggtcag
    39481 ataacggtga cgctaaaaac ccagctctat ctccactagg tgaaaacgtg a
//
LOCUS       DBEROU010000018        37004 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.018, whole genome shotgun sequence.
ACCESSION   DBEROU010000018 DBEROU010000000
VERSION     DBEROU010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 37004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37004
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.018"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            82..315
                     /locus_tag="ACL8CL_000962"
     CDS             82..315
                     /locus_tag="ACL8CL_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HGS1330231.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            315..1649
                     /locus_tag="ACL8CL_000963"
     CDS             315..1649
                     /locus_tag="ACL8CL_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HGS1330232.1"
                     /translation="MTDERIHILRDILLELHNGASPESVQDRFDATFTGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYESMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTAKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQIFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKAIMKGLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDENGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            1659..1913
                     /locus_tag="ACL8CL_000964"
     CDS             1659..1913
                     /locus_tag="ACL8CL_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HGS1330233.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            2009..2404
                     /locus_tag="ACL8CL_000965"
     CDS             2009..2404
                     /locus_tag="ACL8CL_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HGS1330234.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLDLKGIGPATIKKLKENGIKFK"
     gene            2687..3264
                     /locus_tag="ACL8CL_000966"
                     /pseudo
     CDS             2687..3264
                     /locus_tag="ACL8CL_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
     gene            3251..3811
                     /locus_tag="ACL8CL_000967"
     CDS             3251..3811
                     /locus_tag="ACL8CL_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HGS1330235.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIHAYASLPEVAYPAGFL
                     PVKTLEDEIYYLVHILPNRNQKENFPAGYGIVVKGTDKVIGSVDFNHRHEDDVLEIDY
                     TLHPDYWGRGYVPEAARTLIDLAFKDLGLHKIELTCFGYNLQSKRVAEKLGFTLEARI
                     RDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            3811..4389
                     /locus_tag="ACL8CL_000968"
     CDS             3811..4389
                     /locus_tag="ACL8CL_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS1330236.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYHLIYLKQIQSLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKYKPLKRHIEVSEREIIETAETLQLNSQHQPNELNH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            4382..7033
                     /locus_tag="ACL8CL_000969"
     CDS             4382..7033
                     /locus_tag="ACL8CL_000969"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HGS1330237.1"
                     /translation="MSKELSPKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYATTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VNLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPEFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTEDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNADGEMYVGEEAPEGDGWTQDEDVLDTWFSS
                     ALWPFSTMGWPEVDSEDFKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGRQPFQNVL
                     IHGLIRDEQGRKMSKSLGNGIDPMDVIEKYGADALRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEDDKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAAYPTVNLAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAQSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIYLPLADLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVVNAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            7114..8205
                     /locus_tag="ACL8CL_000970"
     CDS             7114..8205
                     /locus_tag="ACL8CL_000970"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HGS1330238.1"
                     /translation="MGKIVAIDLFSGAGGTTSGLKKSGIDVQVAVEIDSVAVKTYKLN
                     NPEVSVIDKDINVVSGDEVKEYLKIDTDDKLMLVACPPCQGFSTIGTNNENDERNQLV
                     FQFLRLINELNPDFLLMENVVGMTRTRNEKIFKKFLTSIDNNYSVIYDILNTADYGVP
                     QSRKRLVLHGIRKEILDNTNLTLNLPSKTHSNIPELDLLSWINADIIMDLPPIEGGEE
                     YIGEGIYNHVANKLSNLNIERITHIRNNGGSRTSLPKSLELNCHKNRLGHSDVYGILD
                     INKPSITITGGCMSYSKGRFGHPTQNRALSAREAARLQSFDDDYIFVGNRGQLAKQIG
                     NAVPVKLAQASGNYFMSVYREIANGQSDN"
     gene            8414..10018
                     /locus_tag="ACL8CL_000971"
     CDS             8414..10018
                     /locus_tag="ACL8CL_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS1330239.1"
                     /translation="MSEYWFSTNVDQIDEVDGKQCLIYSYYNIKASRNVEVLKGRSGT
                     KKGLDYWEPYAPQKQYEMERLPKNKYIGSSSTDRWDGIEKNVVFCDCKEYVSAFDLFF
                     YHYNFKKISTQRSKQDFIRLRSKPVADILKNNTSSYTRYKKEMVIDNVKVDDKVCEII
                     SEIMDESYTDIQILTHKLYSKGDDIKASKTIWMKKSGKEYSEAFAGTGEARIILLVND
                     IVNAQSNSLILIDEPEISLHPSAIYKFKEFLLQECLNKKHQIIITTHSTQLIKDFPRE
                     AVKLLVKNGEKVDVIENIDYQDAFFELGDVYHSRKMIYVEDRLAKYILEFVITHSGSE
                     NLKQNLVVRYIPGGANQIICNNILNSSYLDSDNHYFWLDGDQNTNVSESNNLMNYLEN
                     GVVISDKIPESDNKNLDDIIKLITGCPIKFNVSGNKGQKNNIELIAKQRSFIDYWAKY
                     VSYLPFPTPEFFLANLCNSVDREGYDFSKDGNGKEYFRKKTQVALGIENITSEDIFQE
                     QRRAVSKIQPESSMFQCIKEKLEALF"
     gene            10039..10180
                     /locus_tag="ACL8CL_000972"
                     /pseudo
     CDS             10039..10180
                     /locus_tag="ACL8CL_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155197.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            10398..11201
                     /locus_tag="ACL8CL_000973"
     CDS             10398..11201
                     /locus_tag="ACL8CL_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HGS1330240.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATVYHHTVDASTEEADLVSLRIVELLSRRGFRFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
     gene            11473..12843
                     /locus_tag="ACL8CL_000974"
                     /pseudo
     CDS             11473..12843
                     /locus_tag="ACL8CL_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397831.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
     gene            12855..14255
                     /locus_tag="ACL8CL_000975"
     CDS             12855..14255
                     /locus_tag="ACL8CL_000975"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGS1330241.1"
                     /translation="MSERKTKTIILVHNKQLLDQWLDRLNCFLTFEEEEAIRYTASGR
                     EKVIGYVGQYGGTKKWLSKLVDVVMIQSLFKLENSQSLLDEYEMMIVDECHHVSALMF
                     EKVVAQFRGKYLYGLTATPERKNGHEPIVFQRIGEILHTADKRETDFKRQLQLRFTSF
                     AHLEIEKTKASNFIQLSDWIATDSARNQLILKDILAQVAEGRNILVLVNRIQQIDVFE
                     KLLKEKEVDGCYIISGKTKVRERTSLLEILEQLDKGFVLLSTGKYIGEGFDLPQLDTL
                     ILAAPFSWKNNLIQYAGRIHRNYKDKSLVRIFDYVDIHVPYLEKMFQKRQVAYRKMDY
                     RAIEGEEKQFVYVDSRYENVLRKDLAGERQECLLVLPYVHQTKLMNFLKEFRISQIEI
                     CIPETVANKAWLDQLKSKKIKVSFTQSKIVTPILLVNKTIVWYGAMPLLGKVDEMTIL
                     RLESASIVSELVAGLR"
     gene            14555..15394
                     /gene="licT"
                     /locus_tag="ACL8CL_000976"
     CDS             14555..15394
                     /gene="licT"
                     /locus_tag="ACL8CL_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator LicT"
                     /protein_id="HGS1330242.1"
                     /translation="MIIKRILNHNAVIAQSKKDIDILLFGRGIAFGRKTGDKVNPIDI
                     EKSFFLKNRDNMTRFTEMFINVPLELVYITEKIINLGKITLGNNFDEIIYINLTDHIS
                     SSIERYKEGIIISNPLRWEISKYYKEEFELGKRALQIIKKELGIELPIDEAAFIALHF
                     VNANLENNFQESYKITEIIMGIEKIIQDFYCTEFNQDSIDYYRFITHIKLFAHRLVEN
                     TTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTEYNYLLTSSELVYLMAHIRRLTK
                     NLD"
     gene            15412..17250
                     /locus_tag="ACL8CL_000977"
     CDS             15412..17250
                     /locus_tag="ACL8CL_000977"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015573 - beta-glucoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015759 - beta-glucoside transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucoside-specific PTS transporter subunit
                     IIABC"
                     /protein_id="HGS1330243.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFVVPVTPASYGSSVVPILIMV
                     WLMKYIEKMIAKLTPAVTKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVKAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            17263..18678
                     /locus_tag="ACL8CL_000978"
     CDS             17263..18678
                     /locus_tag="ACL8CL_000978"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGS1330244.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLIGIDFYHKYKEDITLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWVDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDELNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            19269..20315
                     /gene="pheS"
                     /locus_tag="ACL8CL_000979"
     CDS             19269..20315
                     /gene="pheS"
                     /locus_tag="ACL8CL_000979"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HGS1330245.1"
                     /translation="MSTIEEQLKALREETLASLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            20315..20824
                     /locus_tag="ACL8CL_000980"
     CDS             20315..20824
                     /locus_tag="ACL8CL_000980"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1330246.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTVSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            20901..23306
                     /gene="pheT"
                     /locus_tag="ACL8CL_000981"
     CDS             20901..23306
                     /gene="pheT"
                     /locus_tag="ACL8CL_000981"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HGS1330247.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVESVESPAADLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETNEAAADALSVIIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDN
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASLIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VRVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTVTQKLRRQVRTIAE
                     GAGLTEIITYALTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFTRLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEGALQPAVPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(23374..24374)
                     /locus_tag="ACL8CL_000982"
                     /pseudo
     CDS             complement(23374..24374)
                     /locus_tag="ACL8CL_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            24530..25019
                     /locus_tag="ACL8CL_000983"
                     /pseudo
     CDS             24530..25019
                     /locus_tag="ACL8CL_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            25104..25370
                     /locus_tag="ACL8CL_000984"
     CDS             25104..25370
                     /locus_tag="ACL8CL_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1330248.1"
                     /translation="MEGCLSELFSKEEILESDMRVAIMSELIEARNKQGISQKKLEEV
                     SGVSQPVIARMETGKTSPQLDTVLKVLASLGKTLAVVPLEQGKS"
     gene            25630..27879
                     /gene="metE"
                     /locus_tag="ACL8CL_000985"
     CDS             25630..27879
                     /gene="metE"
                     /locus_tag="ACL8CL_000985"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HGS1330249.1"
                     /translation="MSTTIVGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVSAYQEVFAKLAELGATRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLTVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPAFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEVILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            27943..28809
                     /gene="metF"
                     /locus_tag="ACL8CL_000986"
     CDS             27943..28809
                     /gene="metF"
                     /locus_tag="ACL8CL_000986"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HGS1330250.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNDNIISALQDMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIELIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDNERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDIAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(28991..29260)
                     /locus_tag="ACL8CL_000987"
     CDS             complement(28991..29260)
                     /locus_tag="ACL8CL_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine methyltransferase"
                     /protein_id="HGS1330251.1"
                     /translation="MLGSLSFFLRNETKIQLLKILSRQATQRKIGNRTTERLLLGRFI
                     FFHRVVGKFSFQDTSLERFNTKVSKAFTLIAIGRLAKCFTNSLVK"
     gene            29494..31707
                     /gene="pnp"
                     /locus_tag="ACL8CL_000988"
     CDS             29494..31707
                     /gene="pnp"
                     /locus_tag="ACL8CL_000988"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HGS1330252.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSTDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQVVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            31723..32340
                     /gene="cysE"
                     /locus_tag="ACL8CL_000989"
     CDS             31723..32340
                     /gene="cysE"
                     /locus_tag="ACL8CL_000989"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HGS1330253.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            32352..33236
                     /locus_tag="ACL8CL_000990"
     CDS             32352..33236
                     /locus_tag="ACL8CL_000990"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1330254.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            33318..34661
                     /gene="cysS"
                     /locus_tag="ACL8CL_000991"
     CDS             33318..34661
                     /gene="cysS"
                     /locus_tag="ACL8CL_000991"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HGS1330255.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            34654..35040
                     /locus_tag="ACL8CL_000992"
     CDS             34654..35040
                     /locus_tag="ACL8CL_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HGS1330256.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            35044..35928
                     /locus_tag="ACL8CL_000993"
     CDS             35044..35928
                     /locus_tag="ACL8CL_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1330257.1"
                     /translation="MREKELQDWFPDARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVTDILSDMIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            36065..36664
                     /locus_tag="ACL8CL_000994"
     CDS             36065..36664
                     /locus_tag="ACL8CL_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330258.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGHNFSSVSSS
                     SQTAKYEETGYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKNAIIEKLKEANKNHP
                     AGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVTKYAR"
     gene            36807..36977
                     /locus_tag="ACL8CL_000995"
                     /pseudo
     CDS             36807..36977
                     /locus_tag="ACL8CL_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
BASE COUNT        11501 a         6542 c         8081 g        10880 t
ORIGIN      
        1 ctgttttgag caacctgcgg ctagcttcct agtttgctct ttgattttca ttgagtatta
       61 gaacatacaa tggaggtcgt catggacaat atcatcgatg tgtcaattcc tgttgcagaa
      121 gtggtggaca agcatccaga agtcttggaa attctagtgg agttgggttt taaacccctt
      181 gccaatccct taatgcgcaa tacagttggt cgtaaagtat cacttaaaca gggttctaag
      241 ctagcaggaa ctcctatgga caagattgta cgcacactgg aagcgaatgg ctacgaagtg
      301 attggattag actaatgaca gatgaacgga ttcatatcct acgggatatt ttgttagaat
      361 tgcacaatgg cgcctctcct gagtcggttc aagatcgctt tgatgcgacc tttacgggcg
      421 tgtcagccat cgagatttcc cttatggagc acgagctgat gaactcggat tcgggcgtca
      481 cttttgaaga tgttatggaa ctctgtgatg tccatgccaa tctttttaaa aatgctatca
      541 aaggtgtcga agtttcagat actgagcatc caggtcaccc agttcgtgtc ttcaaagaag
      601 aaaatctggc tctccgtgcg gccttgattc gcattcgtag attgttagat acctatgagt
      661 ctatggaaga cgaggaaatg ctggcggaga tgcgtaaggg tttggtgcgt cagatgggac
      721 ttgtgggtca atttgacatc cattaccaac gtaaggaaga actcttcttt cctatcatgg
      781 agcgctatgg acacgattca cctcccaaag ttatgtgggg agtggatgat cagattaggg
      841 aactctttca aacagctcta acgacagcca agtcactacc agaagtgtca attagcagtg
      901 taaaggaagc ttttgaagct tttgcgacag agtttgaaag tatgattttc aaggaagagt
      961 ccatcctgct catgattctc cttgagtctt ttactcagga tgactggctt cagattgcgg
     1021 aggagagtga tgcctatggc tatgccatca tccgtccgtc agagaaatgg gtgccagaac
     1081 gacagatctt tattgaggaa aagattgcag aggagcctgt acaactagat acggcagaag
     1141 gtcaagttca acaagtcata gatacgccag aaggccattt taccattacc tttaccccta
     1201 aggaaaagga agctgtgctg gaccgccata gtcaacaggc ttttggtaat ggctatcttt
     1261 cagtcgagca ggccaatctc atcctcaatc atctccctat ggagattacc tttgtcaata
     1321 aagaagatat tttccagtat tacaatgaca atacgccagc tgatgagatg attttcaaac
     1381 ggacgccgtc ccaagtcggg cgcaatgtcg aactctgtca tccgcctaag tatttggaca
     1441 aggtcaaggc tatcatgaag ggacttcgtg aggggaccaa ggacaagtat gaaatgtggt
     1501 tcaagtctga gtcgcgagga aagtttgtcc acatcaccta tgctgcagta cacgatgaaa
     1561 acggagaatt ccaaggtgta ctggaatacg ttcaggatat ccagccctac cgtgagattg
     1621 atacggacta tttccgtgga ttagaataag gagaaaaaat gagttacgaa caagaattta
     1681 tgaaggaatt tgaagcttgg gtcaataccc aaatcatgat taacgacatg gcgcacaagg
     1741 aaagtcaaaa agtttacgaa gaagaccagg acgagcgtgc caaagatgcc atgattcgct
     1801 acgagagtcg tttggatgct tatcagttct tgcttggtaa gtttgaaaac ttcaaagtag
     1861 gcaagggatt ccatgatttg ccagaaggct tgtttggtga gcgaaattat taaacgagaa
     1921 agattcttga tttttcacta aaatcttgat agaatgttta tgttaaatcc ttgtcagagc
     1981 agggattttt tattgaaagg attttatcat gtcaaagaaa ctcaatcgta aaaaacaatt
     2041 acgaaatggc ctccgtcgcg caggtgcctt ttcaagtact gtgactaagg ttgtagatga
     2101 gacaaaaaaa gtcgtgaagc gtgcagaaca gtcagcaagc gcagctggta aggctgtttc
     2161 taaaaaagtt gaacaagcag tagaagctac caaagagcaa gctcaaaaag tagctaattc
     2221 tgtagaagat tttgcagcaa acttgggtgg acttccactt gatcgtgcca agactttcta
     2281 tgatgaagga atcaagtctg cttcagattt caaaaactgg actgaaaaag aactccttga
     2341 cttgaaagga atcggcccag ctaccatcaa gaaattgaaa gaaaatggca tcaagttcaa
     2401 gtaatttttc ttgagccttg catttccgag aaaaacttgc tacaatagag ccattagagg
     2461 tgttttgaat cccacatttt acagaaagtg gcagcgctga gaagtccaca aatgtgtcaa
     2521 aactggttgc taatgactga aaaaatgaaa tatttctgtc tttttatttt aaagactaaa
     2581 gatgcgggct tggcccgaaa ttgggtggta ccgcggataa acacattcgt ccctgtcatg
     2641 tagatggcag ggcttttctt ttgtgtctta gtcaaaggag gttgttataa aacaatcatt
     2701 taaaactaat aaactatatt atggttttcc tgtttttatt ttaggatatc aagatcagaa
     2761 ttttagctat aacatcatga cctgtagttc ctcttatagt ctaggagatt gggctgtgat
     2821 tggagtcgtt gcgagagaga atgccgcaga gcagatcaaa caatatcaaa aatttactgt
     2881 gaatatttct gatgaaactt ctatgcttgc gatggagcag gctggtttta tcagtcatca
     2941 ggagaaattg gaacgtttgg gagtgcatta tgaaatttct gaacgaactc agactcctat
     3001 tttagacgcc tgtccacttg ttttagattg tcgggtagat aggattgttg aggaagacgg
     3061 tatttgccac atctttgcca agattcttga gcgacttgtt gccccagaac tcctggatga
     3121 aaagggacat tttaaaaatc aactgtttgc ccaacctatt tcatgggaga tggatatcag
     3181 cgcgtttatt gctatctgga caagcgtgta gatatgaagg gcagtttcat caaaaaagcg
     3241 agaaagaagg atggcaagaa ctgagctgcc agataaaatc gaaacagaac gtctcgtttt
     3301 acgagtccgc acagtggcgg atgctgagga tatccatgcc tatgctagtt tgccagaggt
     3361 cgcctaccca gcaggttttc tgcccgtcaa gaccttggaa gatgagattt actatctggt
     3421 gcatatcctt cccaatcgca atcaaaagga aaatttcccg gctggctacg gaattgtcgt
     3481 caaaggaacc gataaagtca ttggttctgt tgatttcaac catcgccatg aagatgatgt
     3541 gctggaaatc gactatacct tacacccaga ctattggggg cgaggttatg tgccagaagc
     3601 tgcgcgtacc ttgattgact tagcctttaa agatttgggt cttcacaaga ttgaactaac
     3661 ttgctttgga tataaccttc aaagtaaacg agtcgcggaa aagcttggct ttaccctcga
     3721 agctcgcata agagaccgca aagatgctca aggcaactgc tgtgatgatt taagatatgc
     3781 cttgctgaag agtgaatggg aggtgatttg atgcatcttt tcatcattgg tgctccagca
     3841 tcaggaaaaa tgaccattgg tcaagaatta tctcgactga cggatgctac cctattttat
     3901 aaccatcaag ccatcgattt tgcactagaa atctatcagg actatacaga ggaaatgtgg
     3961 gaatttgttc gtggaatcac tttttcattc cttggagcga gtgcaagaaa tcagcgatca
     4021 gtaattttaa cagatgtaat tgatttttca aatcagtatc acctgatata tttaaagcaa
     4081 attcagagtt tgttggatga ttatcatcaa gagattcttt ttgttgagtt ggaaacaagt
     4141 cttgaagagt gcatacgtcg aaatcgaacg gagaatcgat taaagtacaa acccttaaaa
     4201 cgacatattg aggtatctga aagagaaatt atagagaccg ctgaaacact tcaattaaat
     4261 tcccaacatc aaccgaatga gttgaaccac tactttaaaa taaataatac gaatttgtct
     4321 gcagaagaag ctgctaagca gattcaaaat aaaataaaca aaatagagaa aggacacaca
     4381 catgtctaaa gaactttcac ctaaatacaa tccagccgag gttgaggctg gtcgttacca
     4441 aaaatggctt gatgccgatg ttttcaagcc ttcaggcgat caaaaggcta agccttattc
     4501 aatcgtgatt ccaccaccaa acgtaactgg gaaactccac cttggtcacg cttgggatac
     4561 aactttgcaa gatattatca tccgtcaaaa acgcatgcaa ggttttgata ccctttggct
     4621 tcctgggatg gaccacgcag ggattgccac tcaggctaag gttgaggagc gcttgcgtgg
     4681 tgagggcatt tcccgctatg accttggtcg tgagtctttc ttgacgaaag tctgggaatg
     4741 gaaagacgaa tatgccacta ccatcaagga acaatggggc aagatggggc tctctgtaga
     4801 ctattctcgt gagcgtttca ctcttgacga aggtttgtca aaagctgttc gtaaggtctt
     4861 tgtgaacctt tacaagaaag gctggatcta ccgtggtgag tttatcatca actgggaccc
     4921 agcagctcgc acagcccttt ctgatattga ggtgattcac aaggatgtgg aaggtgcctt
     4981 ctaccacatg aattacatgc tggaagatgg ttcacgcgcc cttgaagttg ctacaactcg
     5041 tcctgagact atgtttgggg acgttgcggt tgcggttaat ccagaagacc cgcgctacaa
     5101 ggacttgatt ggtaaaaacg tcatccttcc aatcgctaat aaactcatcc caatcgttgg
     5161 agatgagcac gcagatcctg agtttggtac tggtgtcgtg aaaatcacac ctgcccacga
     5221 tccaaatgac ttcttggttg gccaacgtca taacttgcca caagtcaacg tcatgaacga
     5281 cgacggaact atgaatgagc ttgcctttga attttcaggc atggatcgtt ttgaagctcg
     5341 taaggcagtc gttgctaagt tggaagaaat cggtgccctc gtcaaaatcg aaaaacgtgt
     5401 ccacagtgtt ggtcactcag agcgtacagg tgttgtggtt gaacctcgct tgtctactca
     5461 atggttcgtc aagatggacc aattggctaa gaacgccatt gccaaccaag acacagagga
     5521 caaggtcgaa ttctacccac ctcgtttcaa cgataccttc cttcaatgga tggaaaatgt
     5581 ccacgactgg gttatctctc gtcagctctg gtggggtcac caaatccctg cctggtacaa
     5641 tgctgatggt gaaatgtatg tcggcgaaga agctccagaa ggtgacggat ggactcagga
     5701 cgaagacgtc ttggatactt ggttcagttc tgccctctgg ccattttcaa ccatgggctg
     5761 gcctgaagtc gactcagaag actttaaacg ttatttccca acttcaacct tggtaacagg
     5821 ttacgacatc atcttcttct gggtgtctcg tatgatcttc cagtccttgg aatttactgg
     5881 tcgtcagcca ttccaaaacg tgcttatcca cggtctcatc cgtgacgagc aaggacgcaa
     5941 gatgtctaaa tctctcggta acgggattga cccaatggat gtcatcgaga aatacggtgc
     6001 tgatgccctt cgttggttcc tttcaaacgg ttccgcacca ggtcaagacg ttcgtttctc
     6061 ttacgagaaa atggatgctt catggaactt tattaacaaa atttggaaca tttctcgcta
     6121 tatcctcatg aacaatgaag gtttgacgct ggatgtggcg catgacaatg tcacaaaagt
     6181 tgcaacaggt gaggctggta atgtgacgga ccgctggatt ctccacaatc tcaacgaaac
     6241 cattgcaaaa gttactgaaa actttgataa gttcgagttt ggtgtggctg gtcatatcct
     6301 ttacaacttc atctgggaag aatttgccaa ctggtatgtt gagttgacca aggaagttct
     6361 atacagcgac aatgaagacg ataaggtcat tactcgatct gttctcctct atacgctgga
     6421 caagatcctt cgtctccttc acccaatcat gccattcgtg acagaggaaa tctttggaca
     6481 aatctcagaa ggttctatcg tgacagctgc atacccaact gttaatctag cctttgaaga
     6541 ccttgcggct cacactggtg tggaaagtct caaagacttg attcgtgctg ttcggaatgc
     6601 gcgtgcggaa gtaaacgtag cacaaagcaa acctatcacc atccttgtta agacaagcga
     6661 tagcgacttg gaagccttct ttaacagcaa tgtcaactat atcaaacgct ttacaaatcc
     6721 agaacacttg gaaatcgcat caaccatccc tgcacctgaa ctggctatgt caagtgtcat
     6781 cacaggagca gaaatctacc tgccactggc cgatctcctc aatgtcgaag aagaattggc
     6841 acgtctggaa aaagaacttg ccaaatggca gaaagaactg gacatggttg gtaagaaact
     6901 ctctaacgaa cgcttcgtag tcaacgccaa accagaagtt gtccaaaaag aacgcgacaa
     6961 acaagctgac taccaagcca agtatgatgc gaccgtagca cgtattgatg agatgaagaa
     7021 gttggtgaaa tgatgttaaa aacctaaccc tatcgggttg ggttttacat ttgataatgt
     7081 tataataaaa aggagaaact ttaggagaat actatgggga aaattgttgc tattgattta
     7141 tttagcggtg caggtggcac tacaagtggc cttaaaaaat caggcataga tgttcaagtt
     7201 gctgttgaaa ttgattccgt agctgttaaa acttataagt taaataatcc tgaggtgtct
     7261 gtaatagata aagatataaa tgtagtatca ggagatgaag taaaagagta tcttaaaatt
     7321 gatacagacg ataaactaat gctcgtggca tgcccaccat gtcaaggttt ttcaactatt
     7381 ggtactaata atgaaaatga cgagaggaat caacttgttt ttcaatttct tcgtttgatt
     7441 aatgaattga atcctgattt tctattaatg gaaaatgttg tgggtatgac tagaacaaga
     7501 aatgaaaaaa tctttaaaaa atttttgaca tcaatcgata ataattatag tgttatttat
     7561 gacattttaa atacagctga ttatggagtt cctcaatcta gaaaaagatt ggtgttacac
     7621 ggaattagaa aagaaattct tgataacacg aatttaactt tgaatttgcc ttccaaaact
     7681 cattcaaata taccagaatt ggaccttctt tcatggatta atgcggatat aataatggac
     7741 ttgcctccta ttgaaggtgg tgaagaatat attggagaag gtatctacaa tcatgttgca
     7801 aacaaactat ctaatttaaa tattgagaga attactcata ttagaaataa tgggggaagt
     7861 agaacctctt tgccaaaatc tttagaatta aattgtcata aaaatcgttt aggtcatagt
     7921 gacgtatatg gaatattaga tatcaacaaa ccttccatta ctattacagg agggtgtatg
     7981 tcatattcaa aaggtagatt tgggcaccca actcaaaata gagctttaag tgcaagagaa
     8041 gctgcaagat tacagtcttt tgatgatgac tatatttttg ttggaaatag aggtcaatta
     8101 gctaagcaaa ttggtaatgc agttccagta aaattagctc aagctagtgg aaattatttt
     8161 atgtcagttt atagggagat agcaaatgga caatcagata attgagagtt tagaaggaat
     8221 aactaaaaat ttttttaaaa ataaaaatca attttatcct tatattgatt atatacgatt
     8281 tcctcattac aagagcttat cattaaattc aagaataaat tttaatttcc ctattacttt
     8341 attagttggt agaaatggag taaataaaac ttcaatttta caagctttat atggttctcc
     8401 agccaataaa agtgtgtctg aatattggtt tagtacaaat gttgatcaaa tagatgaagt
     8461 ggatgggaaa caatgtttaa tctattcata ttataacatt aaagcttcta gaaatgtaga
     8521 agttcttaaa ggccgttctg gaacaaaaaa aggattagat tattgggaac catatgctcc
     8581 tcaaaaacaa tatgagatgg aaagattacc caaaaataaa tatattggaa gttcttcgac
     8641 agacagatgg gatggcattg aaaaaaatgt tgtcttttgt gattgtaaag aatatgtttc
     8701 tgcttttgat ttatttttct atcactataa ttttaaaaaa atttctactc aacgtagtaa
     8761 acaagatttt ataagattac gatctaaacc agttgctgat attttaaaga ataatacaag
     8821 tagctatacg cgttataaaa aggaaatggt tatagataat gttaaagttg atgataaagt
     8881 ttgtgaaatt attagtgaaa taatggatga atcttataca gacattcaaa ttttaacaca
     8941 taagttgtat tcaaaaggtg atgatataaa agcttccaaa actatttgga tgaaaaaatc
     9001 tggaaaagaa tattccgagg cttttgcagg aacaggtgag gctagaatta ttttgttagt
     9061 aaatgatata gtaaatgcgc aatcaaattc attaatatta atagatgaac ctgaaatctc
     9121 acttcatccg agtgcaatct ataaatttaa agagttttta cttcaagagt gtttaaataa
     9181 aaaacatcaa attattatca ctacacattc tacacaactt ataaaagatt ttcctagaga
     9241 agccgtgaaa cttttagtga aaaacggaga aaaggtagat gttattgaaa atattgatta
     9301 tcaggatgca ttttttgaat taggtgatgt gtatcattct aggaagatga tttatgttga
     9361 agatagacta gctaaatata ttctagagtt tgttatcact cattcaggta gtgagaatct
     9421 taaacagaat ttagtagtga gatatattcc tggtggagca aatcaaataa tttgtaataa
     9481 tattttaaac tcatcgtatt tagattccga taaccattat ttttggcttg atggagatca
     9541 aaacactaat gttagtgaat caaataattt aatgaactat cttgaaaatg gtgttgttat
     9601 atcagataaa attcctgaat cagataataa aaatcttgat gatattataa aattgataac
     9661 gggatgtcca attaaattta atgtttcagg taataaaggg caaaaaaata atattgaatt
     9721 aattgcgaaa caaagaagct ttatagatta ttgggctaaa tacgtttctt atcttccttt
     9781 cccaacccca gagttttttt tagctaattt gtgtaacagc gtagataggg aaggttatga
     9841 tttttctaaa gatggcaacg gaaaagaata ttttagaaaa aaaactcaag tagcgttagg
     9901 aatagagaat ataacttctg aagatatttt ccaggagcaa agaagagctg tttcaaaaat
     9961 acagccagaa tcatcaatgt ttcagtgtat taaagaaaaa ttagaggcat tgttctaatt
    10021 ccttagatta ggtactacat aacaaatttt ttaaattcaa acccgcacca aaaacaacat
    10081 tgcaactttc ttctcaggta atgtgttggg atttgaacag accaacgagt tcatagtggt
    10141 gtatgccaat gactttgata agtctacgcg tcagacttat tagttcattt atccagatat
    10201 atctcgataa tcatattatt tagtcaaaga acatcccata tgagggcatg aaatgctaag
    10261 tttagcttgc tcactattaa gatgaagtat atatatttca tagaacctat tgtgaaggat
    10321 ttttataatt ttcgccaatt ccatacctat ttcacaattt accgatgaca cagaaaaaca
    10381 attgaaagga tatttctatg caatcaactt acaacattga caatccaaac ttgtcttatg
    10441 aagcgaagcg tgatttatgg cggataggtt ttggtctgca gaaagttgac aatctagtgc
    10501 catcagcgta tatggaatct ttggctgaga aacagtcccg gggtgaactg acttatgagc
    10561 aggtttatga ggatgcaacg gtttatcatc ataccgttga tgcgagtacg gaggaggcag
    10621 acttggtttc tttgcgtatc gtagaactct tgtctcgaag aggttttaga ttcagtcctg
    10681 cgactttact tgctattcat aaggagttgt ttcaagatat atttgaaccc tctattccgg
    10741 tggggcaatt tcgtcagacc aatatcacaa agaatgaacc tgttttgaat ggtgaaagtg
    10801 ttgtgtactc tgattactcc atgattcaaa tgaccttaga ttatgatttt aatcaggaaa
    10861 aacaagttgc atatgcgaca ctaactcagg cggatgtggt taaacaaatc cagcatttta
    10921 tttcaggaat ctggcagatt catccatttc gcgaaggaaa cactcggaca gtaacggtct
    10981 ttttgattca gtatcttcgt gagtttggtt ttgatattga taatacacca tttcagcaac
    11041 attccaaata ttttcgtgat gccttggtat tagataatgc aaagatttta cagcgacgtt
    11101 ctgagttttt aacagctttt tttgaaaatc tcttgctcgg tggtcaaaat gatttgtctt
    11161 cagaaaaaat gtatctagat ttagacctcg atttttcata atccaaatac tgagtaaaca
    11221 ttgaatttta ggcaaaaatg aagtaaatat tctcacaaga aaacgtatat catcaaagtt
    11281 ttaggtcgct tgataatatg cgttttttga attttataga ctgctcgttt aaactctatt
    11341 tacctcgtac attccggagc gagatggact attggtcgca tacaaaatga gtgttattcg
    11401 gattttattt tcgtgtacaa tttcagccat tcttgttata atcagcctat aggaatcaag
    11461 gaggtgattg ttatggctgt ttttgtgtct ttggatggaa ttgtggtaga agtacttgat
    11521 gtcttttctt cttttaatgg ggatagtgag tttttcttgt gtaaacgtct taaagacaag
    11581 agtcagtttg tgatggaacg ctctcggttc gaggagatgt tccaacttca aagtagtcac
    11641 ttgacgacgc aagaaaaatt acaattgttt acctccgtgt ttgctggacg ttatgatgtt
    11701 tatgctaaga attttatcaa tgaacaaggg aaaattcagt attttccttc ctatgattat
    11761 ggttggaagc agttgccacc tgaaaaacgg agtttccaga cattgacgaa ctccgttttg
    11821 aaatctcatt ttcgtgggga ggcagctatc ggtatctttc ctatgcactt agatgatagc
    11881 tgttattttt tggtactgga tttggatgaa ggagattgga aagaagctgg tttaaccatt
    11941 cgaagaatag ccagggaacg ccagatggaa gcccatttag agatttctcg ttcgggttac
    12001 ggactccata tttggttctt ctttgaggaa gcgattctga gtcgaaaggc tcgcctgttt
    12061 ggaaagaaac tattagaatt ggctatgcag gaaagtatgc aactgtcctt tgattctttt
    12121 gatcgcatgt ttccaaatca ggatgttctt cctaaaggtg gatttgataa tttaatttcc
    12181 cttccttttc aaggagaagc ttatcatcaa gggcgaacgg tctttgtgga tgaacagttt
    12241 cagccttatg aagatcaatg gaggtatcta caagaaattc agaggatttc aactgctaaa
    12301 gtggcactgt taatccaaga ggagttaggc aagcaagaat tggataagga gttgaaggtc
    12361 gttttatcca atatgatcca acttgaaaaa tcgtctgtga catccaaggc actttttttc
    12421 ttgaaaaata tggcttcctt ttctaatccc gaattttata gtagattgaa actagaatag
    12481 tacacctctg cttctaaaac attgttagaa atcgatttga ctttcctgat cgatttgtcc
    12541 tgttattatt tcattttact atatttaaag caggctatgc gacagccaat ctatcaaatt
    12601 cctgagcgaa tgtatttatt tggagaatcc gattattatt tatggctgcc aagaggcttg
    12661 ttagatccat tgcaagataa atttaagcag gtagttgtgg aggataggag aaaagtacaa
    12721 aggtctatta gagtagcatt taagggagag tttactttgg agcaagaatt agccctgtca
    12781 gatatgaatt ctaaagaaaa tggtttactt catgcgggac aggttttggg aagaccgttt
    12841 taggtgctgc tcttatctct gaacggaaaa caaaaacaat tattctagtc cataataagc
    12901 aactcttaga ccaatggcta gatcgcttaa actgcttttt gactttcgaa gaggaggagg
    12961 ctatccgtta tacggcatca ggtcgtgaaa aggtaatcgg ctatgttgga cagtacggtg
    13021 ggactaagaa atggctgagt aaactggttg atgtcgttat gattcaatct ctatttaagt
    13081 tggaaaatag tcaaagtctt ttggatgagt atgagatgat gattgtggat gagtgtcatc
    13141 atgtctctgc cttgatgttt gaaaaagttg ttgctcagtt tagagggaag tatctttacg
    13201 gtttgacggc tacgcctgag cgtaagaatg gtcatgagcc tattgttttt cagagaattg
    13261 gtgagatact ccatactgct gataagaggg aaacggattt taaacggcaa ttgcaattaa
    13321 gattcacttc ttttgctcat ttggaaattg aaaagaccaa agcaagtaat tttatacagc
    13381 tcagtgattg gattgctaca gactcagcga ggaatcagtt gattctcaag gatattctag
    13441 cccaagtggc agaaggtcgg aatatcttgg ttctagttaa tcgaattcaa cagatagatg
    13501 tttttgaaaa gttattgaaa gagaaagagg ttgatggctg ttacattatt agcggaaaaa
    13561 ccaaagtccg agagagaaca agtttactgg agatattaga acagctagat aaagggtttg
    13621 tattgttgtc aactggaaaa tacattggcg aaggttttga cttacctcag ttggacacgc
    13681 tgatcttggc agcgcccttt tcttggaaaa ataatttgat tcagtacgcc ggtcggattc
    13741 atagaaacta caaagataag tctttggtac gtattttcga ttatgtggat attcatgttc
    13801 cttatttaga aaagatgttt cagaaacgac aagtagctta tcgaaagatg gattatcgtg
    13861 ccattgaggg tgaggagaaa caattcgttt atgttgatag tagatatgag aatgtgttga
    13921 ggaaggactt agcaggggaa agacaggaat gtctgcttgt tttaccttat gtgcaccaga
    13981 caaaactgat gaattttcta aaagaattta ggattagtca aattgagata tgtataccag
    14041 agacggttgc aaataaagca tggctagacc agttgaagag caagaaaatt aaagtgtctt
    14101 ttactcaatc aaaaatagta acgcctattc ttttggtgaa taagactatt gtttggtatg
    14161 gtgcaatgcc attattaggg aaggtagatg agatgaccat attacgtttg gaatcagcta
    14221 gtatagtttc tgaactagtg gcaggtttac gatagagaaa atttttaaaa atttctatgt
    14281 atgattttca tttttttagt gagactgttg ccattatcac attcgaatca cacaaaataa
    14341 aaaaatttct acaagtactt gacaaataga ttgaaatatc ttaaaatgaa aatggttaca
    14401 gagttattaa ttatttaagc ttcatgtcac cattaaaaat tgaaataaaa ggatgttatc
    14461 actaatacaa gtgagcagga acctatttaa tcacatcgga agaagtttct tgatgttttt
    14521 aagtaggttc cttttatttt aaaagggaaa ttttatgatc ataaaacgaa tactaaacca
    14581 caatgccgta attgcgcaaa gtaaaaaaga tatcgatatt cttctttttg gaaggggaat
    14641 agcttttgga agaaaaactg gagataaagt aaatccaatt gatattgaga aaagtttttt
    14701 tctcaaaaat agagataata tgacccgttt tacagagatg tttattaacg ttcctttgga
    14761 gttggtgtac atcaccgaaa aaataattaa cctaggtaaa ataacattgg gtaataattt
    14821 tgatgaaatt atctatatta atttaacgga tcatatttct tcgagcatag aacgttataa
    14881 agaagggatt attatttcga atcccctacg ctgggaaata tcgaaatatt ataaagaaga
    14941 atttgaactt gggaaaaggg ctttacaaat aataaaaaaa gagttaggta ttgaacttcc
    15001 aattgatgaa gctgcattca tagcgctaca ttttgttaat gctaatttag aaaataattt
    15061 tcaagagtcg tataaaatca ctgaaataat tatgggaatt gagaaaatca ttcaagattt
    15121 ctattgtact gagtttaacc aagattctat tgattattat agattcataa ctcatataaa
    15181 attatttgcc catcgtttgg ttgaaaatac aacttattgt gacgatgatg atgaagactt
    15241 gttagcatta atgaaaaata aatatcctag agaatatgaa tgtggtgaac aggtggcaat
    15301 gtttatacaa acggagtaca attatttgct gacttctagt gaattagttt atttgatggc
    15361 tcatattcgt cgcttaacaa aaaatttaga ttaatagaat aggagaatac catgagttat
    15421 aaagatacgg tacaaaaaat cctcgatgta attggaggcg aaaaaaatgt caatagagtt
    15481 acccattgtg taacacgttt aagattagaa ttaaaagatg aaaatttagt caatgatgat
    15541 gatgtgaaga agataccagg tgtaataggt attatgaaaa agaatggaca atatcaaatt
    15601 atacttggta atgatgtagc taattattat aaagaattcg ttaaacttgg caattttgaa
    15661 tccgattcag ttgttcaagg gcacaaaggg aatattttag aaagaatcat tgagtatatc
    15721 gctggttcca tgactccaat cattccagca atgttagggg gaggtatgct gaaagtcttg
    15781 gtaattattt taccaatgct tggtatattg caatcagatt ctcagactat tgcttttttg
    15841 acattttttg gggatgctcc atattatttc ttaccgctgt tattagctta ttctgcatca
    15901 caaaaattaa aagtaacatc tacattagct atgtctgtag caggtgtact tctccatcca
    15961 aattttgttc aaatggtgca atcagggaat cctcttagtt tatttgttgt acctgtgaca
    16021 ccagctagtt atggttcgtc agtcgttcca attcttatta tggtttggtt gatgaaatat
    16081 attgaaaaaa tgattgctaa attaacacca gctgttacta agagtttttt gcaacctacg
    16141 ctagtattat tagtatcaag ctgtattgcc ttagttgtag tcggacctat tggagtaatt
    16201 gttggtgaag gattatcaaa tctagttggg caaatgtatg gtgtagctgg atggcttaca
    16261 ttagctattc ttggcgctat tatgccattt attgttatga ctggaatgca ttgggctttt
    16321 gcacctattt ttttggcggc atctattgct actccagacg tattaattct tccagcaatg
    16381 ttagggtcaa acttagctca aggggctgct tcgatggctg ttgcattaaa gagtaaaaat
    16441 aataatacaa aacaaattgc ttttgcagca ggtttctcag ccttacttgc agggattacc
    16501 gaacctgcat tatatggtgt gactttaaaa tataaaaaac cgctttatgc agctatgatt
    16561 ggtggtggat tagcgggatt atttgcaggt cttactagtg ttaaagcgta tctatttgct
    16621 gtcccatctt tgatagcgtt gcctcaattt atttattctg atgtgccatc aaatattgta
    16681 aatgctttaa ttgtggcggt catttcggtt gttattacct ttgtattagc ttatatattt
    16741 ggaatcgatg aagaagagag ttctagcaat ttagaagtta aagctggagt ttcaaataaa
    16801 aaaatgatat tttctcctat atcaggagaa atcattccgt taagcgatgt ccaggataaa
    16861 acattttcag ataaactaat tggagacgga gtagcgatta tcccaagtga aggtaaggtt
    16921 tatgcaccat ttgatgggaa aattacaaat atttttccga ctaagcacgc aattggattg
    16981 aagagtgatg agggtgttga gttactaatt catattggat tagatactgt tgagctaaaa
    17041 ggtcaaggtt ttattagtca tgtagaagaa ggagacagag ttttcaaaaa tcagttgatt
    17101 tttgaaatgg acttgaattt aatcaagact aaaggctacg aaacagttac accggtaatt
    17161 gtaacgaata ccaatgattt tctagatgta ttagtattac ctaataatca gacaatcgag
    17221 cattctaagg aattactggt aatattataa tggaggaaag ttatgaccat ttttccagat
    17281 gattttcttt ggggtggagc tgttgcagct aatcaagtag agggagcata taatgaagat
    17341 ggtaagggct tatcagttca ggatgtgtta cccaaaggtg gattaggaga agcaacagaa
    17401 aatcctacag aagataactt aaaattgata ggtattgatt tttatcataa atataaggaa
    17461 gatataacct tgttttctga aatgggcttt aatgtttttc gtacttctat tgcatggagt
    17521 agaatttttc caaaaggaga tgaggaagaa cctaacgaag ctgggttgaa atattatgat
    17581 gaattgtttg atgaactaca tgctcacggg atagaaccac ttgtaactct ttcacactat
    17641 gagactccat tatatttagc aagaaaatat catggatggg ttgataggag aatgattcat
    17701 ttttatgaaa aatttgctcg aacagtttta gaaaggtata aagataaagt taaatattgg
    17761 cttacattta acgaagtaaa ctctgttttg gaattaccat ttactagtgg aggaatagat
    17821 atacctaagg agaatctttc gaaacaagaa ttatatcaag ctatacatca tgaattagtc
    17881 gcctcgagtt tggttacaaa aattgctcgt gagattaatt cagagtttaa ggtggggtgt
    17941 atggtattag ctatgccagc ttatccaatg actccaaatc caaaagatgt atgggcgact
    18001 catgagtatg agaatctaaa ttatctattt tcagatgtgc atgttagagg ttattatccg
    18061 aattatgcaa aaagatattt taaggaacat gacattaaca tagagtttgc agctgaagat
    18121 gcagagttat taaaaaatta tactgtagat tttttatcct ttagctatta catgagtgtg
    18181 actcaatctg ctcttccaac acagtataat tcaggagaag ggaatattat tggtggttta
    18241 gtaaatcctt atttagagtc ttctgagtgg ggatggcaaa ttgatccaat tggactacgt
    18301 ataattttaa atagatatta tgaccgttat caaatcccat tatttatagt agaaaatgga
    18361 ttaggtgcta aagatcaact aataaaagat gaacttaata acttaaccgt ccaagatgat
    18421 tatagaattc agtatatgaa agaacattta ttgcaagttg ctgaagctct acaggatggt
    18481 gttgaaatta tgggctatac gtcttgggga tgtattgact gtgtttctat gtccactgca
    18541 cagctttcta agaggtacgg tctcatttat gttgatcgaa atgatgatgg aaatggcaca
    18601 ttcaatcgat ataagaaaat gtcctttact tggtataaag gagtgattga atcaaatgga
    18661 gaatccttat tcaaatagtt ttttattgat gactagctat tatcacggtt aaggtggcat
    18721 ttcattaaat atgaaataaa gaggtttgga agacatcaaa ttctaaacct ctttatttta
    18781 tagggggatt aattaattta attttgttac agagagccga ttataaatag ttaacttagg
    18841 atagattggt tatatatagt agactgaatc taaaatagta cgaaataatt gctaaaacat
    18901 ttatagaaat taattttact ttcccaatcg atttgttttc atcttatttc aatccgctat
    18961 aaagttagat aagaataagc tgacaagcag aagtaagata ggtcttgcaa gaatggtaaa
    19021 aatctgataa actaagtagt aattgaatag gaatctgaaa gactagtagc ccaagggaag
    19081 tatatcaggg aggagagccg tgactgcaag ctctctatat gaaagctggg tgaattcact
    19141 tgcgcatgga tttagaaatg aaggtctgac ttgtcagatg aaaacggatg gtaccgcgtg
    19201 tcaacgctcc gagtggagtt tttggcatgt ggttttcttt ttatctacga gagactgatg
    19261 gaggaaatat gtcaactatt gaagaacaat taaaagcgct tcgcgaagaa acgctggcta
    19321 gcttgaagca gattactgct ggaaatgaaa aagagatgca agatttgcgt gtctctgtcc
    19381 ttggtaaaaa gggttcgctc actgaaatcc tcaaagggat gaaagatgtt tctgctgaga
    19441 tgcgtccaat catcgggaaa cacgtcaatg aagctcgtga tgtcttgact gctgcttttg
    19501 aagaaacagc taagctcttg gaagaaaaga aagtcgcggc tcaactggct agcgagagta
    19561 tcgatgtgac gcttccaggt cgtccagttg cgactggtca ccgtcacgtt ttgacacaaa
    19621 ccagtgaaga aatcgaagat atcttcatcg gtatgggtta tcaagtcgtg gatggttttg
    19681 aagtggagca agactactat aactttgaac gtatgaacct tccaaaagac cacccagctc
    19741 gtgatatgca ggatactttc tatatcactg aagaaatctt gctccgtacc cacacgtctc
    19801 cagttcaggc gcgtgctatg gatgcccatg atttttctaa aggtcctttg aagatgatct
    19861 cgccagggcg tgtcttccgt cgcgatacgg acgatgcgac ccacagtcac caattccacc
    19921 aaatcgaagg cttggtagtt gggaaaaata tctctatggc tgatcttcaa ggaacgcttc
    19981 agttgattgt ccaaaaaatg tttggtgaag agcgtcaaat tcgtttgcgt ccatcttact
    20041 tcccattcac agagccatct gttgaggtgg atgtttcttg cttcaagtgt ggtggagaag
    20101 gctgtaatgt atgtaagaaa acaggttgga tcgaaattat gggggccggt atggttcacc
    20161 cacgtgtcct tgaaatgagt ggtatcgatg cgactgtata ctctggcttt gcctttggtc
    20221 ttggacaaga gcgtgtagct atgctccgtt atggaatcaa cgatatccgt ggattctacc
    20281 aaggagatgt ccgcttctca gaacagttta aataatgatt agaaaagtag aaatggcaga
    20341 tgttgaggtg ttggctaaaa ttgccaaaca aacctttcgt gaaacctttg cgtatgataa
    20401 tacggaagag cagttacagg aatactttga agaggcttat agtctgaaaa ctgtgtcaac
    20461 tgagttggga aatcctgact ctgaaaccta tttcattatg catgaggagg agatagctgg
    20521 ttttctcaaa gtcaactggg gaagtgctca aactgagaga gaattagagg acgcttttga
    20581 aattcaacgc ctctatgtgc tacaaaaatt ccaaggattt ggactaggta agcaactgtt
    20641 tgaattcgca cttgaacttg ctacaaaaaa tagtttttct tgggcttggt taggtgtttg
    20701 ggagcataat acaaaagctc aagcgtttta taatcgatat ggttttgaaa aatttagcca
    20761 acatcatttt atggttggtc aaaaagtaga tacggattgg ttactgagaa agaaattaag
    20821 gtaagaagag gattcttcta ttgaaatgat aggaaaggaa aagaactaga gtgacaactg
    20881 tcattctgga gaactaaatt atgcttgtat cttataaatg gttaaaagaa ttggtggaca
    20941 ttgatgtgcc atcacaggag ttggctgaaa aaatgtcaac cacagggatc gaggtagaga
    21001 gtgtcgaatc accagctgct gatctctcaa aaattgttgt cggtgaggtc ttgtcttgcg
    21061 aagatgtgcc agagactcac ctccatgttt gtcaggttaa cgttggcgaa gaagaagccc
    21121 gtcagatcgt ttgtggtgcc ccaaatgtgc gtgctgggat caaggtcatg gtggctcttc
    21181 caggagctcg tatcgctgat aactacaaaa tcaaaaaagg aaaaatccgt ggtttggagt
    21241 cacttggaat gatctgttca cttggtgaat tggggatttc tgactcagtt gtgcctaagg
    21301 aattcgcaga tggcatccaa atcttgcctg aggatgccgt gccaggtgag gaagtctttt
    21361 cttacctaga cttggatgat gaaatcatcg aactttccat cacaccaaac cgtgcagatg
    21421 ccctttctat gtgtggagtg gctcacgaag tggcagccat ctatgacaag gcagtcaact
    21481 ttaaagaatt tactctaaca gaaactaatg aagctgcggc agatgccctt tctgtcatca
    21541 ttgagacaga caaggcgcct tactatgcag ctcgtatctt ggacaatgtg accatcgcac
    21601 caagtccaca atggttgcaa aaccttctca tgaacgaagg aatccgtccc atcaataacg
    21661 tagtggacgt gaccaactac atcctgctct attttggtca accaatgcat gcctttgact
    21721 tggataactt tgaagggact gacatccgtg tgcgtgaagc gcgtgctggt gaaaaattgg
    21781 tgaccttgga cggtgaagaa cgtgacttgg acgtgaatga cctagtcatc actgtcgcag
    21841 acaagccagt agcccttgca ggtgtcatgg gtggtcaagc aacagaaatc tctgaaaaat
    21901 ctagtcgtgt tgtccttgaa gctgctgttt tcaatggcaa atctatccgt aagacaagtg
    21961 gtcgcctgaa ccttcgttct gagtcatctt ctcgctttga aaaaggaatt aatgtggcaa
    22021 cagttaatga agcccttgat gcggcagcta gcctgattgc ggaacttgca ggtgcgacgg
    22081 tgcgtaaggg catcgtttca gcgggtgagc ttgatacttc agatgtagaa gtttcttcaa
    22141 cccttgctga tgttaaccgt gtcctcggaa ctgagctgtc ttatgctgat gtagaagacg
    22201 tcttccgtcg tcttggcttt ggtctttctg gaaatgcaga cagctttaca gtcagagtcc
    22261 cacgtcgtcg ttgggatatc acaatcgaag ctgatctctt tgaagaaatc gctcgtatct
    22321 atggatatga tcgcttgcca accagccttc caaaagacga tggtacagct ggtgaattga
    22381 ctgtgacaca aaaactccgc cgtcaagttc gtaccattgc tgaaggagca ggtttgacag
    22441 aaattatcac ctatgctctg acaactcctg aaaaagcagt tgagttcaca gctcaaccaa
    22501 gtaaccttac tgaactcatg tggccaatga ctgtggatcg ttcagtcctc cgtcaaaata
    22561 tgatttcagg gatccttgat accgttgcct acaacgtggc tcgtaagaat aaaaacttgg
    22621 ccctttatga gattggaaaa gtctttgaac aaacaggtaa tccaaaagaa gaacttccaa
    22681 atgagatcaa cagctttgcc tttgccttga caggcttggt tgctgaaaaa gatttccaaa
    22741 cagcagcagt tccagttgat ttcttctatg ctaagggaat ccttgaagcc ctatttactc
    22801 gtttgggact ccaagtaacc tatacagcaa catctgaaat cgctagcctt catccaggtc
    22861 gtacagccgt gatttcactc ggtgaccaag ttcttggttt ccttggccaa gtgcatccag
    22921 tcactgccaa ggcttacgat attccagaaa cgtatgtagc tgagcttaac ctttcagcca
    22981 tcgaaggggc gctccaacct gctgttccat ttgtggaaat caccaaattc ccagcagtca
    23041 gccgtgacgt tgcccttctc ctcaaggcag aagtgactca ccaagaagtt gtagatgcta
    23101 tccaagctgc cggcgtgaaa cgtttgacag atatcaaact ctttgacgtc ttctcaggtg
    23161 aaaaactggg acttggtatg aagtcaatgg cttatagctt gaccttccaa aatccagaag
    23221 acagcttaac ggacgaagaa gtcgcacgct acatggaaaa aatccaagcg tctctcgaag
    23281 aaaaagtcaa tgcagaagtg cgttaaacga tagtaggata caaattcccc ctctagttta
    23341 tgaacactag aggggtgatt ttatataaaa taattaaaat gtgatttctg taaaaataca
    23401 tctatgatca gagttttgaa aacttacaat gtctaaatct ttaacatagt agtggttttt
    23461 aggcaataaa atatgatcta ttgttgcatt aaaaagtttt ggactttggc tattccaagt
    23521 tcctctttca aaaggtggaa gtgcattcaa tgcgtcccta tgagagctta tttttgcaag
    23581 tgctccatga cgcatagttg cattaaaatc acctgcaata atagcctttg gatattttga
    23641 agccaattga ttatgaatga tgtttaagtc ttgcttccag atttccatta aacctggcag
    23701 aggaggagca gtatgcaagg caatgatatc tggtatattt tgttttctcg aatgtaatac
    23761 aattgtcccg aaccgtgttg tatgaaaagt tttagcttct gtatagaaac cataactttt
    23821 cttgacaatc acagtcacag gagctattcc actattggta ggtggagaag tgaaaatatc
    23881 atagttggcc atagaaagtc caacttgatg aaacaatagt ttgattctct ggttttcttg
    23941 ctcacctctg atattggtag ctagttcagg gaatatagcc atatcggcgt caaaatggct
    24001 aaaaattcgc tcaatatgtt gtgcttctat ttgattagcg acgttccaag ttactaattt
    24061 taaggtttta gtcttatttg atgcagaaga aagggtttta tctgttccga ataatagtaa
    24121 tatgagcgat agtaagagca aaatgccaga gtaaaaccgt atttgtttat ttttcttgta
    24181 aaaagatagg aaaaaaataa gaaacttaaa atagcagcca tagttgcagt agctaagggg
    24241 aaggcataaa tctgtgccaa gataggttgg gtagatagcc aaatgttaag attagaagat
    24301 gggaaaaaag aataagttat gatagggata gaaaagagaa tgaccgaaag ggcagagaga
    24361 aatgttttaa tcattgtgtg agtacctcaa tttgcaatgg ttgctcggag ctaatgtgac
    24421 tttgtgactt tacttgattg atattctttt tattgtcttc cttatagatt aagtacaacc
    24481 caagtaaaaa aagtccaatt taagattgaa atgattaaaa ggcacagaga tgcaagacaa
    24541 ttatactaca aaaggtaaac atttgacaat cgatagccgt cgcttaatcg aaagatggaa
    24601 aaaagaagga aaatcaaata gagaagttgc ctctctactt ggaaaagctc ctcaaactat
    24661 ccacactgaa atcaagcgtg ggacagtccg aaaatgtctt ggaaaagggc gcttcaaaga
    24721 ggtttatttt gccgactacg ctcaacagtc ttatgaaaac aatcgcaagc gctcggtcaa
    24781 gaaatcaagc ttgaccaagg aactaaagga aaagattctc cactatacat aaccaaaaat
    24841 tttcgcctga aatgatggtt atggctaaag gggttaacgt gggaatttca accatttact
    24901 attggattca tcgtggaaaa ttggggttaa gcaaacagga tttgctttat cctagaaaag
    24961 gaaaatctgt taagaaacaa gttagtccca attttaaacc cgctgatcaa tctatcgaat
    25021 cataattcgc tcattttttc aaaaaataca tagatctaaa agaaagaggg gtaagtgatg
    25081 aagaatagtg ctattgggag taattggaag gatgtctatc tgagctcttt agcaaggagg
    25141 aaatccttga aagtgatatg cgagtagcta tcatgagcga gttgattgaa gccagaaata
    25201 agcaaggaat cagtcagaaa aagctagagg aagtcagtgg agtgagtcag cctgttatag
    25261 ctaggatgga gacaggtaag actagtccac agttggacac agtcttaaaa gtcctagcta
    25321 gtctaggaaa gacactagca gtcgttccac ttgaacaggg gaaaagttga ctagaatcag
    25381 ttatagtctt ttctaataac aagccatagt cacttataag aattactaat aacgcgtttg
    25441 agcattctaa ctaaaataca gctaaaaaca agttataaaa gggatttgag acgtttgctt
    25501 caagtctttt tcttttgtcc gaaacagata tatagtttca aactatatca aaccctaatt
    25561 ttttacaatt atacagacgc tttcttttct gttaagatag tttcaacaac aaattttgga
    25621 ggacacatca tgtcaactac aatcgtcggt ttccctcgtt tgggcgaatt ccgtgaatta
    25681 aaatttacaa ctgaaaaata ctttagaaaa gaaatctcag aagaagaact tttagcagca
    25741 gcaaaagact tgcgtgctaa acactggaac attgtcaaag aaaaaggcat cactgaaatt
    25801 ccatcaaatg acttttctca ctatgataac ttcctagatg cggctttcct tttcaacgtg
    25861 gtgcctgcat ccgttcaaaa cttggacttg tctgaccttg agcgctactt cgctttggga
    25921 cgtggttacc agggagaaaa aggggacgtt cgcgcccttc caatgaagaa atggttcaac
    25981 actaactacc actacatcgt tcctaaattt gaaaaagaca ctcaagtaaa attggctggt
    26041 cacaagattt tcgatgagtt ccaagaagcc aaagaacttg gattgaacac tcgtcctgtt
    26101 cttgtaggtc ccttcacttt ccttcaattg tcagactttg aagaaggcgt gaaagcagac
    26161 gacttcgtag atagcttagt gtctgcttac caagaagttt ttgctaaatt ggctgaactt
    26221 ggtgcgactc gcatccaatt ggatgaagcg gctcttgtaa aagacttgac agctgaagaa
    26281 aaagctcttt tcttgaacct ttacaacaaa ctcttggctg acaaaaaagg tcttgaagtc
    26341 ttgcttcaaa cttatttcgg tgatgttcgt gacgtatacg ctgaccttgt aaacttacca
    26401 gtagatgcta tcggtcttga ctttgttgaa ggtaagaaaa ctcttgaact cgttaaaggt
    26461 ggcttcccag ctgacaagac tctctatgta ggtattgtca atggtaaaaa catctggcgc
    26521 aacaactacg aaaagagctt gactgttctt gagcaaatcc cagctgaaaa catcgttttg
    26581 acaagctctt gctcacttct tcatgtgcca tttacaactg ctaatgaaga atttgaacca
    26641 gcactcttga accactttgc ctttgcagtt gaaaaattgg atgaaatccg tgacttggat
    26701 gctatccgaa atggtcaagg ttcagaagct cttgcagcca acaaagaact ctttgcgact
    26761 gagcgtgttg gtgaaaatgc tgaacttcgt gcgcgtatcg ctggcttgac agacgcagac
    26821 tacactcgtt tgccagcctt tgcagaacgt gaagctatcc aagaagaagc tttcaaactt
    26881 ccagctcttc caacaacaac gattggttca ttccctcaaa caaaagaagt tcgtgccaaa
    26941 cgtttggctt accgtaaagg tgaattgtct caaaaagagt acgacgcttt ccttgctgaa
    27001 acgattgacg aatggatcaa gtggcaagaa gatattgact ttgatgtcct tgttcacggt
    27061 gaatttgagc gtaatgacat ggttgagtac ttcggtcaaa acttgtcagg atacctcttc
    27121 tctaaaaatg gatgggtaca atcatacggt atgcgtgggg taaaaccacc aatcatctgg
    27181 ggtgatgtca ctcgtcttaa ccctatcact gttaaatggt ctagctatgc acaaagtcgt
    27241 acaaacaaac ctgttaaagg tatgttgact ggacctgtta ccatccttaa ctggtcattc
    27301 ccacgtgaag acatctctat caaggattca actctccaaa tcgcccttgc tatcaaggat
    27361 gaagtacttg accttgaagc tgctggtgtg aaaatcatcc aaatcgacga ggctgctctt
    27421 cgtgaaaaat tgccactccg ccgtagcgac tggtacgaag actaccttga ctgggctatt
    27481 cctgccttcc gcttggtaca ctcaacagta gcgccagaca cacaaatcca cactcacatg
    27541 tgttactcag aatttacaga tatcatccca gctatcgata acatggatgc agacgttatt
    27601 tcctttgaag ctagccgttc aaaccttgaa atcttggacg aactcaaagc gaaaaacttc
    27661 caaacagaag tgggacctgg ggtttacgat atccactcac ctcgtgtacc aaatgaaggc
    27721 gaaatcgaca acacaatcga agtcatcctt gctaaagtgc caagcaagaa agtttggatc
    27781 aaccctgact gtggtttgaa aacacgtggt atcccagaaa caaaagaaag cttgatccgc
    27841 cttgttgaag cagctaaagc tgcgcgtgag aaattgtaag attggatttc tctccataca
    27901 gtggttgttc aaaaagaaat caactagaaa aggttattac atatgtcacg ccaaacaccg
    27961 tcactctcat ttgaagtgtt ccctccaaac ccagccgtgg gtaatgataa cattatttca
    28021 gccttgcagg atatgcagga gttggctccc cattttatca gtgtaactgc cagcaataat
    28081 aaatttaata tcaaggaaac gacggttcgt ttggctgact ttatccaaaa tgatttggcg
    28141 attccgacca ttgcccactt gccagctatt tatctaacta aggacaaggt tgctgaaact
    28201 attgctgact tggacaaagt tggggtgcag aaaatcttgg ctcttcgtgg ggatattatt
    28261 ccagacgtgg aaccacaaaa ggatttccgc tatgcaaccg acttgattga gcttatcaag
    28321 gaacgagccc ctcactttga tattattgga gcttgctatc cagaagggca tccagattcg
    28381 ccaaatcaga tttcagatat tcaaaatctt aagaagaaag tggatgcagg ctgttcgagc
    28441 ctcgtaactc agctcttctt tgacaatgag cgcttctatg attttcagga caagtgcatc
    28501 ttggctggga ttgatgttcc cattcatgca ggaattatgc caattctgaa tcgaaatcag
    28561 gctctccgac tcttgaagac ttgtgagaat atccatcttc cacgcaaatt taaagccatc
    28621 ttagacaagt atgagcatga ccctgagtcg ctcagagcag caggacttgc ctatgcagtg
    28681 gaccaaatcg tggacttggt aactcaggat attgccggtg tgcatctcta tacgatgaat
    28741 aatgctgata cagcaaaata catccatcaa gcaacccatg ccttgtttaa tcaccagtct
    28801 ctaggataat aaaagcaaac cattcttctc aggtgagggg aatggttcct ttttaatggt
    28861 aaagacctca ctttttaaga aaaatatgat aaaataaatt ctgtacgtac ttgatacaaa
    28921 gatgaaggta ttaaataata caatgcattt aaactgtaag atttaacgac acgagtgagt
    28981 caaatcgatg ttatttgacg agagagttag taaagcattt agctaatcgc ccgatagcga
    29041 ttagcgtgaa agctttagat actttagtat taaacctttc taatgatgta tcttgaaaac
    29101 tgaacttgcc tacaactctg tggaaaaaga taaatctccc taggagcagt cgctccgtcg
    29161 ttcgatttcc tattttcctt tgagttgctt gacggcttag tatcttgagt aattgaattt
    29221 tggtctcatt tcttaggaaa aaagataagc ttcctagcac tcgtcgagtg cgacgtcacc
    29281 ttcctatttt tctacagaaa tgttcggcaa gccgaaccgt ccaaaatatc ttgtgtaatt
    29341 gaacacggcc gaaaagctgt gtaaaaaaga taaactgtct tgtcttcatc gaagacttcg
    29401 tcagtttcct atttttactt tgcttttgac gtccttggta tcttgatctt tgtaggcaag
    29461 gcgtataatt tcatcaatcc aaaggggatt aaaatggcaa aacaagtgtt tcaaacgact
    29521 tttgcgggtc gtgagttaat tgtagagact ggtcaggttg ctaagcaagc aaatggatct
    29581 gttgtcgtac gttacggtga gtcaactgtc ttgactgctg ccgttatgtc taagaaaatg
    29641 gcaactgggg atttcttccc actccaagtc aactacgaag aaaaaatgta tgcggctggg
    29701 aagtttcctg gtggctttat gaaacgtgaa ggacgtcctt caacagatgc gaccttgaca
    29761 gcgcgtttga ttgaccgtcc gattcgtcct atgtttgcgg aaggtttccg taatgaggtc
    29821 caagtcatca atacagtgct ttcttatgat gaaaatgcat ctgcaccaat ggctgctatg
    29881 tttggttcat ctttggcact gtctatttca gatattccat ttgacggacc aattgctggg
    29941 gtacaagtgg gatatgtaga tggccaaatc atcatcaacc caagtcaaga acaagcagag
    30001 caatctcttc ttgaattgac agtagctgga accaagcacg ctatcaacat ggtagagtct
    30061 ggtgccaaag aattgtcaga agaaatcatg ttggaagcgc ttcttaaagg gcacgaagct
    30121 gtcaaagaat tgattgcctt ccaagaagaa atcgttgctg ctgtcggtaa agaaaaagca
    30181 gaagtggaat tgcttcacgt ggatgctgaa ttgcaagctg aaatcattgc agcctacaac
    30241 agtgacctcc aaaaggcagt tcaagtagaa gagaaattgg cccgtgaagc tgcgactcaa
    30301 gtagtgaaag accaagtgac tgccgtttac gaagaaaaat atgcggacca cgaagaattt
    30361 gaccgtatta tgcgtgatgt ggctgaaatc ttggaacaaa tggaacacgc agaagtgcga
    30421 cgtttaatta cagaagacaa ggtgcgtcct gatggtcgta aggtcgatga aatccgtcct
    30481 ttggatgcgg ttgttgactt ccttcctcgt gtacatggtt caggtctctt tactcgtggg
    30541 caaactcaag ctctttcagt cttgaccttg gctccgatgg gagaaactca aatcattgat
    30601 ggtttggatc cagagtacaa gaaacgcttt atgcaccact ataacttccc tcaatattct
    30661 gtaggggaaa caggtcgtta cggtgcgcca ggtcgtcgtg aaatcggtca cggtgccctt
    30721 ggtgagcgtg ctcttgctca agtcttgcca agcttggaag aattcccata cgctatccgt
    30781 ctagtagcag aagttttgga atcaaacggt tcttcatctc aagcttctat ctgtgcggga
    30841 actcttgccc ttatggctgg tggtgtgcca atcaaggcgc cagtagctgg tattgctatg
    30901 ggacttatct cagatggaaa taactacaca gtattgacag atatccaagg tttggaagat
    30961 cactttggag atatggactt caaggttgca ggtactcgtg atgggattac agcccttcaa
    31021 atggatatca agattcaagg gattactgca gaaatcttga cggaggctct tgctcaagcc
    31081 aagaaagcgc gttttgaaat ccttgatgtc attgaagcaa ccattccaga agttcgtcca
    31141 gaattggctc caactgctcc gaaaattgat acgatcaaga ttgatgtgga caagattaag
    31201 attgtcatcg gtaagggtgg agaaaccatc gacaagatta tcgctgaaac aggtgttaag
    31261 attgatatag acgaagaagg aaatgtgtct atctactcta gtgaccaaga tgctattaac
    31321 cgtgccaaag aaattattgc tggtttggtt cgtgaagcca aagtggatga agtttaccgt
    31381 gctaaagtcg ttcgtatcga gaaatttggt gcctttgtta acctctttga taagacagat
    31441 gcccttgttc atatctctga gatggcttgg actcgtacca atcgtgtaga ggatttggta
    31501 gaaatcgggg atgaagttga tgttaaggtt atcaaaattg atgaaaaagg ccgtatcgat
    31561 gcctctatga aggctcttct acctcgtccg ccaaaacctg agcatgatga aaaaggtgaa
    31621 aagtctgagc gccctcaccg cccacgtcat caaaaggatt acaaacctaa gaaagaattt
    31681 acagaaacat caaaagattc agaataagaa aaggagaaat gtatggggtg gtggcgcgaa
    31741 accattgata ttgtaaaaga aaatgatcca gcggcccgca ccactttgga ggttttgctg
    31801 acttatccag gtgtcaaggc cttggcggcc caccgtctct cgcattttct ctggaagcac
    31861 ggcttcaaac tattagctcg tatgtacagt cagttttggc gcttttggac tcagattgag
    31921 attcatccag gagcccagat tgattcaggt gtttttattg accatggttc tggtctggtg
    31981 attggagaga cagcgattgt tgaaaaaggc gttcttctct atcacggagt gactctcggg
    32041 ggaacaggga aagactgtgg caaacgccat ccgactgtac gaaagggagc cctcatatca
    32101 gcccatgccc aagttatcgg gcctgtggaa attggtgaaa atgccaaggt cggtgctgca
    32161 gcagttgtcg tagcagatgt acctagtgac gtgacggttg tcggtattcc ggccaagatt
    32221 gtccgtcttc atggtaagaa agatgagcct gtcattcacg aagtcgaaga aaaacgagag
    32281 tattatgtca ataaactcga gcaggctaaa gatgccagtc acagatcgtc tggtttgtag
    32341 aggataccta tatgattcaa gcaagaaaca agttaagcca agaggagcta tctgaggcga
    32401 aaaaagtaat taactgttgc caaaactatg acggtaccta tcgcgatccc tatctctcta
    32461 acatgcttaa ttttgaccca aacatgcccg cctttttcct ttattatgaa aaaggcgaac
    32521 ttgttggttt attaactgtc tatgcagatg accaagatgt agaagtgacg atactggttc
    32581 atccaggtca tcgccgtcag gggattgcgc gtgcattgtt tactagtttt gagagagaaa
    32641 cagcttcttt ccccattcgg tcagtcactt ttcagacaga acgtattttt ttagagaatc
    32701 atcctgactt tgccagtaac tggggactaa tcgaggatga agagacagaa acttggttag
    32761 gtaaggatag aagaccttat ccgttagcaa atgtttccca tcttgaagtt ttgttagcag
    32821 atagttcgta tcaggatcaa attagtcaat taaaatttca ggcattttca gaggaacatg
    32881 aatcgagaga agttgtggat agatatgtcg ctgaagctct gaaagatcca gaaagccgac
    32941 tatatatttt attaaaagct ggtcaggtta ttggaacttg tacggttgat ttatcgacta
    33001 atacgaatta cctctacggt ttagcaatat tggaacctga acgtggaaaa ggctatggaa
    33061 gctacttagc aaaatccctc gtcaaccaac taattgagca aaatgacaag gaatttcaga
    33121 ttgcagtgga agatagcaat gtaggtgcca aacgtttgta tgaaaaaatt ggctttgtca
    33181 aacagactca ggtggtttat ctgaatgaga aaggagcaag ggattccgaa gtgtagagat
    33241 attcggactg aaatttactt gaacttttag tgatgaaact aattgttctt ggatttcagc
    33301 tttcctgatt atgatttatg attaaaatct atgacaccat gtctcgtgat ttgcgagaat
    33361 ttgtcccgat tgaggacggc aagatcaaga tgtatgtttg tgggccaacg gtgtacaact
    33421 atatccacgt aggaaacgcc cgttcgacgg tagcttttga taccattcgt cgctattttg
    33481 agtaccgtgg gtataaggtt gcctatattt ccaattttac agatgtggat gataagatta
    33541 tcaaccgtgc cagggaagaa ggtatcacgc ctcaggaggt tgcggataag tacatcgctg
    33601 cctttcgtga ggatgtgacg gcattgggcg tgaaacctgc gactcgccat ccgcgtgtag
    33661 tggagtttat ggcagacatc atccgttttg tggaagactt gatcgagaaa ggctttgcct
    33721 atgagagtca aggggatgtc tatttccgtg tagaaaaatc ccacaactat gctaaattgg
    33781 ctaataaaac cttggaagat ttggagctag gtgcttcagg tcgtaccgat gaagaaacgg
    33841 ctcgtaagga aaatcctgta gactttgctc tatggaaatc ttccaaacca ggtgagattt
    33901 cttgggatag tccttgggga cctggacgtc cgggttggca tattgagtgt tcagtcatgt
    33961 cgacagagat tttaggtgat accattgata tccacggtgg tggagctgac ctagagtttc
    34021 cacaccacac caatgaaatt gcccagtctg aagctaaaac aggcaaggct tttgctaact
    34081 actggatgca caatggcttt gtcaatatcg acaatgtcaa aatgtctaag tccttgggta
    34141 acttcattac ggtacacgat gcccttaaaa ctcttgatgg gcaagtactt cgtttcttct
    34201 ttgcgaccca gcactaccgt aagcctatca acttcacgga aaaggcagtt cgcgatgccg
    34261 agaccaatct caagtatctc aagaatactt acgaacaacc atttacagga aatgtggatg
    34321 cccaagagtt acaaaacttt aaagataagt ttgtagcagc tatggatgaa gattttaatg
    34381 ctgccaacgg tatcacagtt gtctttgaaa tggccaaatg gatcaactca gggaactatg
    34441 atgcaagtgt caagcaagct cttgcagata tgttagaaat ttttggaatt gtctttgttg
    34501 aggaagtttt ggatgcagag attgaagact tgattcaaaa acgccaagag gcgcgtgcca
    34561 atcgtgactt tgcgacagca gaccaaatcc gtgaccaatt ggttactcaa ggaattaagc
    34621 tccttgatac caaggatgga gtgaggtgga cacgtgattg atgtcaatct cattaacggg
    34681 attgcgctag cctttgaggg ggatgcggtg tattctatgt atattcgccg tcacctcatc
    34741 ctcaaaggta tgaccaaacc caataaactc catcaagaag caaccaagta tgtgtcagcc
    34801 aaggctcagg ctcgcctgat tgctctcatg ttggaggagc aggtcctaac ggaaaaagaa
    34861 gaagaaatct acaaacgtgg ccgcaatacc aatagccaca caaaggctaa aaatgcagat
    34921 gtcgtgactt atcgtatgtc cacgggattt gaagcggtta tgggctatct ccatatgact
    34981 gagaatctgg aacgtcttga gagtttggtt tcatggtgca tccaaaaagt ggagggctag
    35041 aacatgaggg aaaaagaact acaagactgg tttcctgatg ctcggatttc agaccaacca
    35101 gtagagaaag agggctatct cacgctccct ttagcttctc agcagtggat tttgctggag
    35161 gaagctgggc tcagcgagcg tgaaaagcag ttggttgccc ttttgaccca gcaggagcag
    35221 gctcgttcgc taaacccttg gtattcctat ctggttgagg gcaagggaca ggcaccgcaa
    35281 gtttttaaaa agattcagtt ggtttattgc catctttctt attttcagca ggaaaatctg
    35341 gcttcttggc tagatatgat gcggactctt tttccgaatt gtcagacagt gctacaggtc
    35401 ggagctcagg attatgtttt cgtgcttcaa caagacaaat acacttctgt aacagatatt
    35461 ttaagtgata tgattgaagc ggttgagtat gactttggac ttcgtctttc tatcatgttg
    35521 ggtcaggttt ggtctcagac aggacatcaa gccctatcag acttaatcaa agctgagcgg
    35581 gatttgttca agacatggtg gcgtcagggt caccaaggtg ttcatacttt ttctcagctc
    35641 tatctttgga gtatgggaga aagactcgtg gacttgaagc caattaaaga atgtctacac
    35701 cagatgattt tggatcaaga tcagattcag gaaatcattc tctctctttg ggaaaatagt
    35761 gctgttctca ctaaaacagc ccagcaactc tatctgcacc gcaattctct ccaatacaag
    35821 attgataaat gggaagagtt gacagggctt cagttgaaag agttgaccga cctgaccttg
    35881 tgttatcaat tgattttagg ttctttgtca actatagttg gtttgtaaag aagttaatat
    35941 ttggagaaga ggattgccat cttctccatt tttatgtgca gagttatagt ggcttgatgc
    36001 tgggaaagta cactgtgact gctaaaacat tcctagaagc tgctttgatt tccctaatct
    36061 atttatgcaa attttatgtt attttactat aacagttgtt gctaagccaa ataatagtgg
    36121 ggaagttcat ttagacgtaa gcattgaaga taatcaggga ggtagtgggc ataatttcag
    36181 ttctgtttca agtagctcac aaacagctaa atatgaagaa actggttata ataacaattc
    36241 atcattatat ataacgattg ataaaacgtc tgatgcaaca gctcttttga aattaaagtt
    36301 gaataatgtt gataatcaac ctgctactga agttcctagt tcaggaatta ctgtaaaatt
    36361 aaatgctaaa gataatgctg gaaactggac aagtgcttcg aataaaaaag aaataacagt
    36421 aaaaattgtt tctgctaaac cgacatatcc agacaaaatc ttagtgaaaa atcctgataa
    36481 tataaaagat acagaaaaaa atgccattat tgaaaaattg aaagaggcaa ataaaaatca
    36541 tccagcagga gctccaacct ttgctaaagg tgaaggagag catgcaaatg atattgtagc
    36601 aacttattca gatggtacaa cttattatgt accgttaaat gatgtgacaa aatatgcgag
    36661 gtagtggctg taccactcac ttattcacct ccccgtgatt tgtagtagtg ataggctttc
    36721 tcactattat tataaaacaa aataaagatc acaacacttt ttcattctgt gttgtgcctt
    36781 gagtgaaacg aaaggaatga attataaata tgaaaagtat agtcactagc atagatgagc
    36841 gcttgcgtac tcgcctacga gtgattatct ggaagcaatg gaagaagaaa tcgagacgat
    36901 tatggggatt gcttaagtta ggggttccta aatggatagc agataaggta tctggctggg
    36961 gcgaccacta tcaattgtag taaaatgaaa taagaatagg acga
//
LOCUS       DBEROU010000019        36947 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.019, whole genome shotgun sequence.
ACCESSION   DBEROU010000019 DBEROU010000000
VERSION     DBEROU010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 36947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36947
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.019"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            85..465
                     /locus_tag="ACL8CL_000996"
     CDS             85..465
                     /locus_tag="ACL8CL_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330259.1"
                     /translation="MKLKTLALISGIVGLVGGILLLIGPFVLLGTAVNTAATTLNGGA
                     TAGAFSGVALLLNALKIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGGVSLIPFLGW
                     VGGILAIIGGSLFLATLKKFKSEE"
     gene            1000..1692
                     /locus_tag="ACL8CL_000997"
     CDS             1000..1692
                     /locus_tag="ACL8CL_000997"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HGS1330260.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            1745..4157
                     /locus_tag="ACL8CL_000998"
                     /pseudo
     CDS             1745..4157
                     /locus_tag="ACL8CL_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
     gene            4407..5108
                     /locus_tag="ACL8CL_000999"
     CDS             4407..5108
                     /locus_tag="ACL8CL_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330261.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANCDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIQMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            5110..7818
                     /locus_tag="ACL8CL_001000"
     CDS             5110..7818
                     /locus_tag="ACL8CL_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGS1330262.1"
                     /translation="MIKRKTYWKDLVQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEDLEQTLSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAS
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIITKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSLLASVLPAYLVAWRELHAEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMNQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(7914..8405)
                     /gene="tpx"
                     /locus_tag="ACL8CL_001001"
     CDS             complement(7914..8405)
                     /gene="tpx"
                     /locus_tag="ACL8CL_001001"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HGS1330263.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEDLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(8528..9457)
                     /gene="psaA"
                     /locus_tag="ACL8CL_001002"
     CDS             complement(8528..9457)
                     /gene="psaA"
                     /locus_tag="ACL8CL_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HGS1330264.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            complement(9489..10337)
                     /locus_tag="ACL8CL_001003"
     CDS             complement(9489..10337)
                     /locus_tag="ACL8CL_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease subunit"
                     /protein_id="HGS1330265.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            complement(10334..11056)
                     /locus_tag="ACL8CL_001004"
     CDS             complement(10334..11056)
                     /locus_tag="ACL8CL_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGS1330266.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKAKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            11356..13248
                     /gene="pepO"
                     /locus_tag="ACL8CL_001005"
     CDS             11356..13248
                     /gene="pepO"
                     /locus_tag="ACL8CL_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HGS1330267.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPILSLLKEFQELETFADF
                     IAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEEAWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
     gene            complement(13371..14000)
                     /locus_tag="ACL8CL_001006"
     CDS             complement(13371..14000)
                     /locus_tag="ACL8CL_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGS1330268.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKHLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFRFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGPATTIAHEKAFNPFF"
     gene            14994..17216
                     /locus_tag="ACL8CL_001007"
     CDS             14994..17216
                     /locus_tag="ACL8CL_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HGS1330269.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IIANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            17246..17689
                     /gene="dtd"
                     /locus_tag="ACL8CL_001008"
     CDS             17246..17689
                     /gene="dtd"
                     /locus_tag="ACL8CL_001008"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HGS1330270.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKKGNRPAFTGAAKLDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(18161..18574)
                     /locus_tag="ACL8CL_001009"
     CDS             complement(18161..18574)
                     /locus_tag="ACL8CL_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330271.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFIIRLETSFEWLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMP
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            complement(18705..19412)
                     /locus_tag="ACL8CL_001010"
     CDS             complement(18705..19412)
                     /locus_tag="ACL8CL_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0034257 - nicotinamide riboside
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter family
                     protein"
                     /protein_id="HGS1330272.1"
                     /translation="MKKSLKIFATSKWFDLFGVALVVGIAIASGYLNSRLDKFVDWGP
                     WTALVPFGLISVTNVGISMLSTRFTGKLSKWGNYFSIVNTILSGAIDYILGNKAAIIT
                     YPVTFLIYTFAIKKWKASQEGRPNQMSQKQVKLTVIIISIIAFLFAFVTNYIGYEGKM
                     NLLAYVTTIAFALSLIANALNALKLTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFY
                     ILNAIGALFVWNDEEVR"
     gene            complement(19532..20182)
                     /locus_tag="ACL8CL_001011"
     CDS             complement(19532..20182)
                     /locus_tag="ACL8CL_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HGS1330273.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     NFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
     gene            20294..21136
                     /locus_tag="ACL8CL_001012"
     CDS             20294..21136
                     /locus_tag="ACL8CL_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS1330274.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADAQQLGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            21212..21649
                     /locus_tag="ACL8CL_001013"
     CDS             21212..21649
                     /locus_tag="ACL8CL_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HGS1330275.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            21717..22019
                     /locus_tag="ACL8CL_001014"
     CDS             21717..22019
                     /locus_tag="ACL8CL_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330276.1"
                     /translation="MYLVIGIVLAFIVSFWKDNRSLWNPVLFLLSLISSYFYLSYLFY
                     KNGYENVQLAFYVFAFVLLPFLLFLSGIFLIYNGVILLKREGRSKPHYLSMLFDFY"
     gene            complement(22110..23183)
                     /locus_tag="ACL8CL_001015"
     CDS             complement(22110..23183)
                     /locus_tag="ACL8CL_001015"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HGS1330277.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPRDPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSQQVDT
                     TFKEWVATVPDEELQLYFDLFFGTILEAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     ERETLGRLTQLLIDTRYQAWKNR"
     gene            23339..24016
                     /locus_tag="ACL8CL_001016"
     CDS             23339..24016
                     /locus_tag="ACL8CL_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1330278.1"
                     /translation="MHKILLIEDDQVIRQQVGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            24009..24983
                     /locus_tag="ACL8CL_001017"
     CDS             24009..24983
                     /locus_tag="ACL8CL_001017"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1330279.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGFYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(25032..26975)
                     /gene="thrS"
                     /locus_tag="ACL8CL_001018"
     CDS             complement(25032..26975)
                     /gene="thrS"
                     /locus_tag="ACL8CL_001018"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HGS1330280.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPAIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELASGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(27033..27209)
                     /locus_tag="ACL8CL_001019"
     CDS             complement(27033..27209)
                     /locus_tag="ACL8CL_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HGS1330281.1"
                     /translation="MTKQGRYYDETPYTLEQKLSENIWWLLELSQRLDIDILTEMENF
                     LSDKEKQLNVRTRK"
     gene            complement(27216..27356)
                     /locus_tag="ACL8CL_001020"
     CDS             complement(27216..27356)
                     /locus_tag="ACL8CL_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like protein"
                     /protein_id="HGS1330282.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDFKWTVEEDLLALSNDIGN
                     FQ"
     gene            complement(27787..27987)
                     /locus_tag="ACL8CL_001021"
     CDS             complement(27787..27987)
                     /locus_tag="ACL8CL_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330283.1"
                     /translation="MRRFYSHLPYYLVILFFYWPLYELFLLVVSDPLTLKELYINNLL
                     FFTPLVILIISLLYSYRFRFSL"
     gene            28226..28855
                     /locus_tag="ACL8CL_001022"
     CDS             28226..28855
                     /locus_tag="ACL8CL_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS1330284.1"
                     /translation="MKLAVIAANGQAGKAIVEEAVKRGHEVTAIVRSENKSQAESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVVGGAGS
                     LYIDETKTTRLLDTSDFPEEFKSLAKDQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGNYILAGEIFTTNEKGISQISYADYAIGLVDELEKGHHIKERISLLEK"
     gene            28996..29265
                     /gene="rpsO"
                     /locus_tag="ACL8CL_001023"
     CDS             28996..29265
                     /gene="rpsO"
                     /locus_tag="ACL8CL_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HGS1330285.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            30081..30695
                     /locus_tag="ACL8CL_001024"
     CDS             30081..30695
                     /locus_tag="ACL8CL_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HGS1330286.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(30987..31736)
                     /locus_tag="ACL8CL_001025"
     CDS             complement(30987..31736)
                     /locus_tag="ACL8CL_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HGS1330287.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            31869..34205
                     /locus_tag="ACL8CL_001026"
     CDS             31869..34205
                     /locus_tag="ACL8CL_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HGS1330288.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFQGQVVYPMLSNLPFSSDRKWGAMELEGLGIVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLNY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELIALAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GSSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEIATLTGQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            34307..34984
                     /locus_tag="ACL8CL_001027"
     CDS             34307..34984
                     /locus_tag="ACL8CL_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine(22)-N(1))-methyltransferase TrmK"
                     /protein_id="HGS1330289.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLSKEVSPVFVQKWQKEAEKLEFALGQIPEKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            34971..35768
                     /locus_tag="ACL8CL_001028"
     CDS             34971..35768
                     /locus_tag="ACL8CL_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HGS1330290.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLGIEETTYLQETGPERGIGRIGNIQPQTFWELAQQVKQVFDLDS
                     LRMVHYQEDDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIATLLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            35785..36888
                     /locus_tag="ACL8CL_001029"
     CDS             35785..36888
                     /locus_tag="ACL8CL_001029"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS1330291.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQKIDFYQRSGVFLLKKDESKLE
                     ELYQLALQRREESPLIGQLAILDQASANELFPGLQGFDRLLYASGGARVDGHLLVTRL
                     LEASQVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPELAGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPLNYPIENYVKRVKSE"
BASE COUNT        10780 a         6930 c         8479 g        10758 t
ORIGIN      
        1 tattttataa tatcagaaaa tacttttttt aaaagcaaat atgatacaat tttatttgaa
       61 aaaaataaaa aaggagattt tattatgaaa ttaaaaacac ttgctttaat tagtggtatc
      121 gtcggtcttg tgggaggaat tttacttctt attggtcctt ttgtcttgtt gggaacagcg
      181 gtaaacacag ctgctacaac tcttaatgga ggagctactg caggggcttt ttcaggtgtt
      241 gccttactct tgaatgcctt gaagattgca aatcttgttc ttggtatcat tgctattgtt
      301 tactataaag gagataagcg tgtaggtgca gctccgtctg tactaatgat tgtttctggt
      361 ggagttagtc tcattccatt cttaggatgg gttgggggga ttcttgctat tatcggagga
      421 tctctattcc ttgcaacatt gaagaaattc aaatcagaag aataaaaggt attttagcat
      481 gaaaagaaca aaaaagttta tcggtatagg agtagctcta ttatctcttt cttttctagt
      541 tgcatgtgga acataaagtt caaagaatac ttcaacaagt aatgatgaga agacagtagc
      601 aacatccaat agttcaaaag aaacaatcac tttcgataca ccggttgtaa cagacaatgc
      661 gattgaatca atacgcactt atgcagatta tatagatctt tataaaaata tttttgatga
      721 ttattttact aaagctgagg aaggtttcaa aggcacagct atggaaaata atgactcgtt
      781 tactaaacta aaagagtcaa ctcaaaaatt attcgatgcg cagaaaaaaa ggttaaataa
      841 tgaagataga atagaaacaa ccaaaaacaa tgtgattgcc aaacattgtc aaacagtcct
      901 ttcctttttg gttttgacta gcttttttgt gaaaaattgt gtaaaataga atagataaac
      961 gaggggaaac ctcggaaaat ttaaaggaga atccatctaa tggtaaaatt ggtttttgct
     1021 cgccacggtg agtctgaatg gaacaaagct aaccttttca ctggttgggc tgatgttgat
     1081 ttgtctgaaa aaggtacaca acaagcgatt gacgctggta aattgatcaa agaagctggt
     1141 atcgaatttg accaagctta cacttcagta ttgaaacgtg ctatcaaaac aactaacttg
     1201 gctcttgaag cttctgacca attgtgggtt ccagttgaaa aatcatggcg cttgaacgaa
     1261 cgtcactacg gtggtttgac tggtaaaaac aaagctgaag ctgctgaaca atttggtgat
     1321 gagcaagttc acatctggcg tcgttcatac gatgtattgc ctccaaacat ggaccgtgat
     1381 gatgagcact cagctcacac agaccgtcgt tacgcttcac ttgacgactc agttatccca
     1441 gatgctgaaa acttgaaagt gactttggaa cgtgctcttc cattctggga agataaaatc
     1501 gctccagctc ttaaagatgg taaaaacgta ttcgtaggag ctcacggtaa ctcaatccgt
     1561 gcccttgtaa aacacatcaa aggtttgtca gatgatgaga tcatggacgt ggaaatccct
     1621 aacttcccac cattggtatt cgaattcgac gaaaaattga acgttgtttc tgaatactac
     1681 cttggaaaat aaaaaattgt aagcctagga ttgatttcta ggctttttat gttagtatgg
     1741 aagtatgata aggaataaaa aaacaagatt atgtactggc ctacaagcaa ccagtttcaa
     1801 ccacttacat gggttgggaa gaagaagctt taccgatagg taatggttct ttaggagcaa
     1861 aagtatttgg ccttataggg gctgaacgga ttcaatttaa tgaaaaaagt ctctggtctg
     1921 gaggtccact tcctgatagt tcagattatc agggtggaaa tcttcaggat cagtatgttt
     1981 ttttagctga gattcggcag gctttggaga agagagatta caatctggct aaggaactgg
     2041 ctgagcagca cctaattggg ccaaaaacga gtcaatatgg gacctatctg tcttttgggg
     2101 atattcacat tgagttcagc cagcaaggta cgactttgtc tcaggtgacg gactatcaga
     2161 gacagttgaa tattagtaag gcacttgcga cgacttctta tgtctataag ggaacgcgat
     2221 ttgaacgtga agcttttgcg agttttccag atgatctctt ggttcaatgt tttactaagg
     2281 aagggttgga aactctagat tttactatag aactatcctt gacctgtgat ttggcttcta
     2341 atggaaagta tgagcaggaa aaatctgatt acaaggagtg taagttggat attactgatt
     2401 ctcatatctt gatgaaggga agagttaagg ataatgatct gcggtttgct agttatctag
     2461 cttgggaaac ggatggagat attagagttt ggtcagatag ggttcagata tcaggagcca
     2521 gttatgccaa tctcttcttg gccgctaaga cggattttgc ccaaaatcct gctagcaatt
     2581 atcgcaagaa actagattta gagcaacagg tgatagactt ggtggacaca gctaaagaaa
     2641 agggctatac ccaattgaaa tcaaggcata tcgaggacta ccaagcctta ttccagcgtg
     2701 ttcaattgga tttggaagct gatgttgacg catccactac agatgatttg ttaaaaaatt
     2761 ataagccaca agaagggcag gctttggagg agctgttctt ccagtatgga cggtatttat
     2821 tgattagttc gtccagagac tgcccagatg ctctaccagc taacctacag ggagtctgga
     2881 atgcggtcga caatcctcct tggaattcgg actatcactt aaatgtcaat ctgcagatga
     2941 attattggcc agcctatgtt accaatcttc tagagacggt ctttccagtc atcaactatg
     3001 tagatgattt gcgtgtctat ggtcgtctag cggctttaaa gtatgcagga atcgtctctc
     3061 agaaaggtga ggaaaatggt tggttggttc atactcaagc gactcccttt ggttggacgg
     3121 cacctggttg ggattactat tggggttggt caccagctgc caatgcgtgg atgatgcaaa
     3181 ccgtttatga agcctattca ttttataggg accaagacta tcttagggag aaaatttatc
     3241 ccatgttgag ggaaacggtt cgtttttgga atgccttttt acataaggat cagcaggcgc
     3301 agcgttgggt gtcttctccg tcttattccc cagaacatgg gccgatttcg attggcaata
     3361 cctatgacca atctctgatt tggcagttat ttcatgattt tattcaggct gctcaggaat
     3421 tgggactgga tgaggacttg ttgactgagg ttaaggagaa gtctgattta ctaaatcctt
     3481 tgcaaatcac tcaatctggt cgaatcaggg agtggtatga ggaggaagag cagtattttc
     3541 aaaatgagaa agtggaggcc cagcatcggc acgcttccca tctagtggga ctctatcctg
     3601 gcaatctctt tagctacaag ggacaagagt atattgaagc ggcgcgtgct agcctcaatg
     3661 atcgtggaga tggcggcaca ggctggtcca aggctaataa gatcaatctc tgggcgcgtt
     3721 tgggagatgg caatcgagcc cataaattat tggcagagca gttaaagaca tccaccttgc
     3781 aaaatctttg gtgtagccat cctccttttc agatagatgg taattttggt gctactagtg
     3841 gcatggcaga aatgttactc cagtctcatg cagcttatct ggtacctcta gctgccctac
     3901 ctgatgcttg gtcaacaggt tctgtttcag gcttaatggc acgtggacat tttgaagtga
     3961 gcatgagctg ggaagataaa aaactcttac agttgaccat tttatcaagg agtggaggag
     4021 atttgcgagt ttcttatcca gatattgaga agagtgtgat taaaatgaat caagaaaaaa
     4081 taaaagcgaa atgcatgggg aaagattgta tttcggtggc aacagcagaa ggtgatcttg
     4141 ttcaatttta tttttaagaa gatgttataa ggcagtaatt tgaaactgcc ttttaataag
     4201 gatttaagaa tataagcagt tttcaactag ttgaaaaaac gttataatga taataggaag
     4261 taatactcaa tgaaaatcaa agagcacaaa ctaggaagct agccgcaggt tgctcaaaac
     4321 agtgttttga ggttgcagat ggaagctgac gtggtttgaa gagagatttt cgaggagtat
     4381 aatttgtttg atagagggtg ggtctgatgg cttatattga gatgaaacac tgttacaagc
     4441 gttatcaggt tggggacacg gagattgtgg ccaattgtga tgtgaatttt gagattgaaa
     4501 agggggagct ggttattatc cttggtgctt caggtgcagg caagtcaaca gttcttaacc
     4561 ttcttggggg aatggatacc aatgatgaag gggaaatctg gattgatggt gttaatattg
     4621 cggattatag ttcccaccag cgcaccaatt accgtagaaa tgatgtgggg tttgtttttc
     4681 agttttataa tctagtttct aatctgacag ctaaggaaaa tgtggaactg gcttctgaaa
     4741 ttgtgacaga tgccttgaat cctgatcaag tcttgacaga tgtaggtctg gctcatcgtc
     4801 tcaataactt tccagcccag ctttctggag gggagcaaca gcgagtctcc attgcacgcg
     4861 cggtagccaa aaatcctaaa attctccttt gtgatgaacc gactggagcc ttggattatc
     4921 agacgggcaa gcaggttttg aaaattctcc aagacatgtc tcgtcaaaag ggagcgacgg
     4981 tgatcatcgt gactcataat ggagctttgg cgcccattgc tgatcgcgtg attcaaatgc
     5041 acgatgccag tgtcaaggat gtggtgctca accagcatcc tcaggatatt gacagtttgg
     5101 agtactagca tgatcaagcg aaaaacttat tggaaggact tagttcagtc cttcacaggc
     5161 tccaaggggc gttttttatc catcttgatc ctgatgatgt tgggatctct agccttagta
     5221 ggcctcaaag taaccagtcc caacatggag gcgacagcta atgcttattt aacaactgct
     5281 caaaccttgg atttggcagt catgtctaac tatggcttgg atcaagcaga ccaagaagaa
     5341 ctaaaacaga cggagggcgc agaggtcgag tttggctatt tgacagatgt gactatggat
     5401 aatgggcagg atgccattcg gctgtactcc aaaccagagc gaatttcaac ctttcagcta
     5461 agaaagggac gacttcctca gtcagacaag gaaatcgctt tggccactca tttgcaaggc
     5521 caatacagcg tgggacagga gattagtttt aaagaaaaag aagagggtca ttcctcttta
     5581 aaagaccata cttataccat tactggtttt gtggattcgg ctgaaatcct ctcccagcga
     5641 gatatgggct acgcaggaag tggaagtggg actctgacag cctatggggt gattttacct
     5701 agtcagtttg atcagaaagt ctacaatata gctcgtttga aatatcaaga tttagcaggt
     5761 ttaaatgcct tttcatcagc ttatgaagaa aaatccaagc aacatcagga agaccttgaa
     5821 caaactttat cagataatgg caaggtacgt ctgcaacttt tgaaaaaaga aggacaagag
     5881 tctctagaca aggggcaaga gacccttgac aaggctcaga ctaatttgca ggaaggcaag
     5941 cgtcgtttag cagctgctca agctcgtata caggctcaag aaagtcaact agccttgttt
     6001 cctcaagttc agagagagca ggctagtgct caacttaccc aagccaagca ggaattgggc
     6061 aaggaagagg ataaactaaa gcaagctgaa caaaatctag cccaagaaaa ggaaaaatta
     6121 gaaaaacatc agcaagtctt ggacgatttg gcggagccaa ggtatcaggt ctataatcgt
     6181 cagaccatgc caggtggtca gggctatctt atgtatagca atgcttcatc cagtattcga
     6241 gcagtgggca atatctttcc tgtggtactt tatgccgtag catccatggt gacctttacg
     6301 accatgactc gctttgtaga cgaagagcga actcatgcag ggatttttaa ggccttgggt
     6361 taccgtagta aggatattat caccaagttt ctcctttatg gactagtagc tgggactgtc
     6421 ggaacggctc taggtagtat acttggtcat tatttgctag ccagtgtaat ttcaagtgtc
     6481 attacaaaag gcatggtggt gggagaaacc cagattcagt tctattggac ctatagctta
     6541 ctagctcttg tcttgagctt gttggcgagt gtgttaccag cctatctggt ggcttggagg
     6601 gaacttcatg ccgaagcagc ccagcttcta cttcctaaac ctcctgtcaa aggagctaaa
     6661 atcttattgg agcgtatcgg ttttatctgg cgtcgtctca gttttactca taaggtaaca
     6721 gcccgcaaca tctttcgtta taagcagaga atgttgatga caatctttgg tgtggcaggt
     6781 tctgtagctc tgctctttgc aggtttggga atccaatctt ctgtagcagg agttccgtct
     6841 aaacagtttc aacaaatcca acagtatcag atgcttgtct ctgaaaatcc tagtgcgacc
     6901 aatcaggaca aggtagagct agcagaagta ttgaaagggc aggagatact agcctaccag
     6961 aaaatctatt ctaaaacgct agacaaggat ttcaaaggca aggctggtct tcaaaacatt
     7021 actcttatga tgatagagaa ggaagatttg actcccttta tccatcttca acatcatcag
     7081 caggagctga cattaaaaga tggcatcgtt attacagcta aactcgccca gctggcaggt
     7141 gtcaaggttg ggcagacttt agaaattgaa ggtaaggaac taaaggtcgc tgctattact
     7201 gagaactacg ttggtcactt tatttatatg agtcaggcta gctatgagca actttacgga
     7261 cagctacccc aagccaacac ttatctggtc tcattaaggg ataccagtgc aactagtatc
     7321 gaaagtcagg cgggcttgct tatgaatcaa tctgcggtgt ccagcgttgt ccaaaatgct
     7381 tcagccattc gactcttcga ctctatcgct agctcactca atcagaccat gaccatcttg
     7441 gtcatcgtat cggttctatt agctattgtc atcctttaca atctgaccaa tatcaacgta
     7501 gctgagagaa tccgtgaact ctccactacc aaggttctcg gttttcataa taatgaagtc
     7561 acactctaca tttaccgtga gacgattgtg ctatcccttg tgggaatcgt acttggtctg
     7621 gtagctggtt tctatttaca ccaatttttg attcaaatga tttcgcctgc gactattctc
     7681 ttttatccgc aggtaggctg ggaagtctat gtaatcccag tggcagcagt aagcatcatt
     7741 ttgaccttgc ttggtttctt cgtcaattat tatctgagaa aggttgatat gttagaagcc
     7801 ctgaaatctg tagagtaagg tagttatttt tagctgattg aacttctatt tactaatatt
     7861 caaaaatcct ccgtttcaaa gagcagggaa ctctttgtga cagaggattt tttctatagg
     7921 gctttagcag ctgcaattgc ggcttcgaag tttggctcag aattgatatt atccacgtat
     7981 tcaacgtagc gaatcgtatt gtcagtatcg aggacaaaga ctgcgcgtgc taataggtgc
     8041 cattcgttga tcaagagggc ataatcgcgc ccgaaagaat ggtcaaagta gtctgaaagc
     8101 ataatggcat tgtcaaggtc ttcagcaccg caccaacgtt tttgagcaaa aggtaggtcc
     8161 attgaaacag tcaatacgac cgtgttgtcc agtccagcca attcttcatt aaaacgacgt
     8221 gtttgagttg agcagatgcc tgtatcgata gaaggaacga cactcaagac ttttttcttg
     8281 ccatcaaaat cagccagaga ttttttagaa agatctgttg tagtaagaga aaaatcaagc
     8341 gccttgtcgc cgacttgtag ttgtttacct gtaaagctca caggatttcc gagaaaagtt
     8401 accataggat actccaatct tttttcttcc attgtatctg aaacagtcag aattttccaa
     8461 tgatttgacc ggaaatgtgg gcatagaaaa aacgccagct cacatgagaa tgacgttttt
     8521 cagaggctta ttttgccaat ccttcagcaa tcttgtcaag gttgtatttc atcatgctgt
     8581 agtagctgtc gccttcttta ccttgttctg cgatagagtc agtaaagatt tgtgcgtaga
     8641 ttgggatgtt tgtgtcttga gaaacagttt tcattggacg gtcatccaca cttgattcta
     8701 caaagagtga tggaactttt gtttggcgaa gtttttcaac caaggtcttg atttgttcag
     8761 gagttccttc ttcttcagta ttgatttccc agatgtaggc acttggaaca ccataggctt
     8821 tagagaagta tttgaatgct ccttcgctgg ttacaatgag tttcttttca gcagggatct
     8881 tattaaattt atccttactt tctttatcaa gtttgtctaa cttatcagta tattctttga
     8941 gatttttttc atagaattct ttattgttag ggtctttggc gctcaattgt ttggcgatat
     9001 ttttagcaaa aataataccg ttttcaaggt taagccaagc gtgtgggtct tcttttcctt
     9061 tttcattttg accttcaagg tagataacat caacgccgtc gctgactgcg aagtagtctt
     9121 tgttttcagt tttcttggca ttttctacca attttgtaaa ccaagcattg ccacctgttt
     9181 caaggttgat accgttatag aaaatcaaat cagcctcaga agttttctta acgtcttcag
     9241 gaagtggttc gtattcgtgt gggtcttgcc caatcggaac gatactatga aggtcaattt
     9301 tgtcaccagc aatattttta gtaatatcag cgatgattga gtttgtagca acaactttta
     9361 gtttttgacc agaagttgta tctttttttc cgctagcaca tgctacaaga atgattgcag
     9421 aaagaaagag aacgagtaat gtacctaatt ttttcattag atcctccaat ttattagggc
     9481 tttgcccctt attttaacaa atgtttattt ttcagtttca aatatcgttg tttgggagcg
     9541 ataaagaagc taatgagaaa gaaactagca gctgtaagca cgatactaga acctgccgca
     9601 acattaaaac tatagccaat aaagagtccc aaaactgaag cagtagctcc gaaggttgag
     9661 gaaaggaaaa tcatactttt cagactatta gcatacagat aagcagttgc agctggggta
     9721 atcagcatgg ctacaatcag gatagttccg acactttgca tggctgtcac agacacgaga
     9781 gtcaggagta ccatgagaag gtagtgatag aaattgacag gcattcccat ggctttagcc
     9841 aagagttcat caaaggaagt tatcaagagt tgcttgaaga aaatccagat taacaagagg
     9901 atggctgccc ccacacccat agtaataaac atatccgtat cttggacggc caggatatta
     9961 ccaaaaagga tatggaaaag gtcagttgaa cttttagcga caccaatcaa gatgataccg
    10021 agggctaaga aagaagaaaa ggtaatgccg atggcggtat cgcttttgat aatcgagttt
    10081 cctttgatgt aggtaatgat gatggcagct agcaatccaa agacaatggc tccgataaag
    10141 aagtcaaggc ccaagatgaa ggagagggct acacctggta agacagcatg tgaaatggca
    10201 tctcccatga gtgacatccc gcgtagaata atgaaacatc ccacagctcc agctacaatc
    10261 ccgacgacaa tagctgttat caaggcattt tgtaggaaat ggaatttttg caatccatcg
    10321 ataaattctg caatcatagg tcacctccat tgaaaaagag ttgattaccg taagcttctt
    10381 ttagattggt ttcggtaaaa gtttcttttg ttggaccaaa ggcaatcact tctcgattga
    10441 caagtaagac ttgatcgaag tagtggggaa tcttgctgag gtcgtggtga acgatgagaa
    10501 ccgtcttccc agcttttttc aaatctctca gcgtattcat gatgatttcc tcactgacag
    10561 agtcaatccc agcaaagggt tcatccaaga ggatatagtc ggcttcctgc accaaacatc
    10621 tggcaatcaa gacccgctgg aattgacctc cagacagttg actaatttga cgttcagcgt
    10681 agtcagctag gccgacgatt tcaagggcct cttgcacttt cttccaatgt ttagccttta
    10741 aacttcgaaa gagaggaata gagggaaata gtcctaacga gacgcattcc ttgaccttga
    10801 tgggaaagtt gtagtcgata ttgatttttt gttcgacata ggcaattcgg tgtaaggatt
    10861 ttttaacttc cttgtcatcg agaaatgcct gaccttgatg tgggataatt cccagcatac
    10921 cttttaatag tgttgatttc ccagcgccgt ttggaccaat gatgccggta attgttggtc
    10981 catggagcac tagtgaaata tccttaagtg ccaacgtttc tttgtaggag acactgaggt
    11041 tttcgatacg tatcataaac ttgtattcct cctgtctctt aatatacatt aaaaaaaaaa
    11101 ttaagtcaag ttaatttttg aaaaaattaa aataataact gaaaaataga ttctaaagat
    11161 aactttcagg ataaatttct aaattataaa acgcatagta tgcatagtat caagtgtaaa
    11221 aaacttggaa ttatgcgttt tatcatggaa agattcttta taatagctaa aaaataatag
    11281 ttaaatggtt actaattgca ttccctagtg atttttgtta agataaaggc aaatacaaat
    11341 gaaagcgaga acaagatgac acgttatcaa gatgattttt atgatgctat caatggagaa
    11401 tggcaacaga cagctgaaat cccagcagat aagtctcaaa caggaggttt tgttgattta
    11461 gaccaggaaa ttgaagactt gatgctggcg acaacagaca agtggttagc aggtgaagaa
    11521 gtgcctgagg atgctatctt ggaaaacttt gtcaaatacc accgcctagt tcgtgatttt
    11581 gacaagagag aagctgacgg tatcacacct atcttatcac tccttaaaga atttcaagaa
    11641 ttggagactt ttgcggattt tatagctaaa ctagcagagt ttgagcttgc aggaaaacca
    11701 aacttccttc cttttggtgt atcgccagac tttatggatg ctagaatcaa tgttctatgg
    11761 gctagcgctc caagcacaat cttgccagat acgacctact atgcagaaga acatcctcag
    11821 cgcgaagagc tcttgactct ttggaaagaa agcagcgcaa atctcctcaa ggcttatgat
    11881 ttctctgatg aagaaattga agacttgcta gaaaaaagac ttgaattgga ccgccgagtt
    11941 gcggcagtgg tgctctctaa tgaagaaagt tcagaatatg ctaaactcta tcatccatat
    12001 tcttacgaag atttcaagaa attcgcgcct gccctacctt tggatgactt cttcaaagca
    12061 gttattgggc aattaccaga caaggttatt gtagacgagg aacgtttctg gcaagcagca
    12121 gagcaattct acagtgagga agcctggtct ctccttaaag caaccttgat tttgagtgtt
    12181 gtcaatcttt caaccagcta tttaacagag gatatccgtg ttttgtctgg tgcctacagc
    12241 cgtgcccttt ctggagttcc agaggcaaaa gataaggtca aagcagctta tcatctagca
    12301 caagaacctt tcaagcaagc cctgggtctt tggtacgccc gtgagaagtt ctctccagaa
    12361 gccaaggcgg atgtggagaa aaaagtggca accatgattg atgtctataa ggagcgtctg
    12421 cttaagaatg actggctcac tccagaaacc tgtaaacagg ctatcgtgaa gctcaatgtg
    12481 atcaaacctt atattggcta tccagaagaa ttgcctgcac gttacaagga taaggtagtg
    12541 aatgaaactg ccagtctttt tgagaatgct ctagcctttg cgcgtgtgga aatcaagcac
    12601 agttggagta agtggaacca gcctgtagat tataaggaat ggggcatgcc tgctcatatg
    12661 gtcaatgcct actacaatcc tcagaagaac ctgattgtct ttccagcggc cattttacag
    12721 gcgcctttct atgacttgca tcagtcatct tctgctaact acggtggtat tggggcagtg
    12781 attgcccatg aaatttccca cgcctttgat actaacgggg cttcctttga cgaaaatggt
    12841 agcctcaagg attggtggac agagagcgac tatgctgcct tcaaggagaa aacacaaaaa
    12901 gtcattgacc aatttgatgg acaggattct tatggagcaa ccattaacgg taaattgact
    12961 gtatcagaaa acgtggctga cttgggagga atcgcagcag cgcttgaagc agctaagaga
    13021 gaagcagact tctcagcaga agagttcttc tacaacttcg gtcgcatctg gcgcatgaaa
    13081 ggtcgaccag aatttatgaa acttttggct agcgtcgatg tgcacgcacc agccaaactc
    13141 cgtgtcaatg tgcaagtacc aaacttcgac gatttcttta caacctatga tgtcaaagaa
    13201 ggagacggaa tgtggcgttc accagaggag cgcgtgatta tttggtaata caaaaaatct
    13261 agtcatcaga aaaaaggcat ccaatcacgt gtgaaaacct tgataggatg ccttttgtaa
    13321 tagaaagatt tgctggatag tttacttaaa tttcgattgt catcatcttg ctagaaaaag
    13381 ggattgaagg ccttttcgtg agcgatagta gtagctggac catgtcctgg atagacatcg
    13441 tagtttggta gggtgaagag ttgggtctgg atactatgaa ggagttgctc catgctacca
    13501 gtcggaaggt cggtccgtcc gatagtttcg cggaatagag catctcccgt caagactagg
    13561 tgagcatcag gaaagactag ggaaacacca ccgatagagt gccctggggt cggtagaacc
    13621 ttaaaacgaa attcctctag ttggtattct tcgtgaaaga caaaggtgtg ttctgcaggt
    13681 tttgtgacca catctgccat atcatcgtgg cgagggagac cggagagatt atcgacagga
    13741 gtgtagagcc agctggcttc gctctctgcg atatagacag gaggattgcc aaacgtctcg
    13801 cgaaccaagt ccagactcat gatatggtca taatgggcgt gggtcaagag aatagcacag
    13861 atcggtttgt tgatcttctc gattgtctga cgaatggctt cccaatgact accaggatcg
    13921 acgacgatga ggtgcttttc gccttctaga taataggtat tttcataggc aacaggattc
    13981 acggttttat ggattttcat actagctcca atctcaaaga atgtactaat actcttcgaa
    14041 aatctcttca aaccgcgtca actttatctg caacctcaaa gcagtgcttt gaacagcctg
    14101 cggctagctt tctagtttgc tctttgattt tcattgagta taaattaagt atagcacagt
    14161 tagggagaat aggtaaggat ttaaaatgaa cttaaaaacc agcatgacta gatgaagaca
    14221 ggctggttat caatttatca atattggaaa ggtgtcaatt gcccttcata ggttgcataa
    14281 actccgtcat tagcttgttt gatagacgtg atgtttagag tgctaccgta gttggcttct
    14341 catttgtcgc tatattcact ataagttatc ctactgggca ggttctggtc gttagcatag
    14401 tattgaacga gttttttata gctttgctgg ctgaatagga acaggcagca aactcctaac
    14461 attaggagag caaagataga aatagcagtt tttttcatag gtattctcct tataaactaa
    14521 aggatagaag aaagtatctt gaggattttt atactcttca aaaatctctt caaaccgcgt
    14581 caacgtcgcc ttgccgtata tatgtgactg actttgtcag tcttatctac aacctcaaag
    14641 cagtgctttg agcaacctgc ggctagtttc ctagtttgct ctttgatttt cattgagtat
    14701 taattccaaa atccagtcaa ggtaccagtg tatgtgatac gagttccacc gtttatcttt
    14761 cgtatcttca atggaattgg ggaaatcaga aagtctttgg gttacaatat ggtttccgtt
    14821 cttctatctc tccttctatc acctaagttt aaaatctaac ttcgtagtat attatgggcc
    14881 aataattcag aattatcaag caattttgaa ttattttttt cttattctgt tccaatctag
    14941 ttttcatatt cttaaaaaag tgataaaatg gtaggaagaa ttggagagta gagatgccga
    15001 aagaagtgaa tttaacaggc gaagaagttg tcgctttaac caaagaatat ttaacggaag
    15061 aggatgttca ttttgtccat aaggccttgg tctatgctgt tgaatgccac agtggtcaat
    15121 atcgcaaatc aggcgagcct tatatcattc accctatcca agtggcaggt attttagcta
    15181 agctaaagct ggatgctgta acagtagctt gtggattctt gcatgatgtg gtggaagata
    15241 cagatgcgac cttggacgat ttggaaagag agtttggtcc tgatgtgcgg gtgattgttg
    15301 acggagttac caagcttggc aaggtcgagt acaaatcgat cgaggagcaa ttagcggaaa
    15361 atcatcgcaa gatgctcatg gccatgtctg aggacatccg cgttattttg gtcaaactgt
    15421 ctgaccgctt gcacaatatg cggaccctga aacatcttcg aaaagacaag caggagcgta
    15481 tttccaaaga aaccatggaa atctatgccc cacttgccca tcgtttgggg atttccagtg
    15541 tcaaatggga attagaagac ttgtctttcc gttatctcaa tccaacggag ttttacaaga
    15601 ttacccatat gatgaaggaa aagcgcaggg agcgtgaggc cttggtggat gaggtagtca
    15661 caaaattaga ggagtacacg acagaacgtc acttgaaagg gaagatttat ggtcgtccca
    15721 agcatattta ctcaattttc cgcaaaatgc aggacaagag aaaacggttt gaggaaatct
    15781 atgatctgat tgctattcgt tgtattttag atacccaaag tgatgtttat gccatgcttg
    15841 gttacgtgca tgaattttgg aaaccgatgc caggtcgctt caaagactat attgccaacc
    15901 gcaaggccaa tggttatcag tctatccata cgactgttta tggaccaaaa gggccgattg
    15961 aattccagat tcgaaccaag gaaatgcacg aggtggctga gtacggggtt gcggctcact
    16021 gggcttataa gaaaggtata aaggggcaag ttaacagcaa ggaatcagct attggaatga
    16081 actggatcaa ggagatgatg gagctccaag accaggctga tgatgctaag gaatttgtgg
    16141 actctgttaa ggaaaactat ttggctgagg agatttacgt ttttacccca gatggagctg
    16201 tccgttccct tcccaaagat tcaggaccga ttgattttgc ctacgaaatc cataccaagg
    16261 tcggtgaaaa agcaactggt gccaaggtca atggccgcat ggttccactg acaaccaagt
    16321 taaagacagg ggatcaggtt gaaattatcg ccaacccgaa ctcctttgga cctagccgtg
    16381 actggctcaa tatggtcaag actagcaagg cgcgcaataa gattcgccag ttctttaaaa
    16441 accaagataa ggaattgtct gtcaacaagg gtcgtgagat gctgatggct cagttccaag
    16501 aaaatggcta tgtggcaaat aaatttatgg acaagcgcca catggatcaa gttctgcaaa
    16561 agaccagtta caagacagaa gactccctct ttgcggccat tggttttggg gaaatcggtg
    16621 cgattaccgt ctttaaccgt ctgactgaaa aggaacgccg tgaggaagag cgtgccaagg
    16681 ccaaggctga ggcagaggag cttgtcaaag gtggcgaggt caaggttgaa aataaagaaa
    16741 ctctcaaggt caagcatgag gggggagtgg ttattgaagg tgcttctggt ctcctagtgc
    16801 ggattgctaa gtgttgtaac cccgtgcctg gtgacgatat tgttggctac attaccaagg
    16861 gtcgtggtgt ggctattcac cgtgtggact gtatgaacct gcgtgcccaa gaaaactacg
    16921 agcaacgtct ccttgatgtg gaatgggaag accagtactc tagctcaaat aaggagtata
    16981 tggcccatat cgatatctac ggtctcaacc gtacaggact gttgaacgat gtactgcaag
    17041 ttctttcaaa tacaaccaag aatatttcaa cggtcaatgc ccaaccaacc aaggatatga
    17101 agtttgctaa tatccatgtg tccttcggta ttgccaacct ctctacactg accacggttg
    17161 tcgataaaat taagagtgtg ccagaagttt actctgtcaa acggaccaac ggctagttgt
    17221 cctagctctt actagaaagg ctattatgaa aatcattatc caacgggtta aaaaagccca
    17281 agtgagtata gaaggccaga ttcagggaaa aatcaatcag ggacttttat tgctggttgg
    17341 tgttggacca gaggaccaag aggaagattt ggactatgct gtgagaaaac tggtcaatat
    17401 gcggattttt tcagacgcag aaggcaagat gaacctgtct gtcaaagata ttgaaggaga
    17461 aatcctctct atttctcagt ttaccctctt tgcggatact aagaaaggca atcgtccagc
    17521 ctttacaggg gcagctaaac ttgatatggc atcagacttc tatgatgctt tcaatcaaaa
    17581 attagcgcaa gaagtgcccg ttcagacagg catctttgga gcagatatgc aggttgagct
    17641 ggttaataac ggacctgtta ccattatcct agatactaaa aagagataag aaagaccaag
    17701 cccagtcggc ttggtctttc tcatctatca taaaatactc caaaaagaaa tcggttcttg
    17761 atatgcttgg gggaagtctc ttttcaggct ttggcagatg cgataggaag ggatgagatg
    17821 tcctaggatg aggagagttc cctgaaggaa aagtggaata tcacttaaag acaccaagga
    17881 gagaatcatc aggctatccc ataggagaat ttgtaaagca aaacgccaga atctcggtct
    17941 aatctgggaa tagagttaac taaagtggtc acaaagttgg tttgaaagat aggtcaatag
    18001 cacaggatga aagaaaatga gccaaccgct ataaatcaaa atccagtccc aagtaagccc
    18061 cccttttaaa tgtcaaaaat gctagcatga ttccatcaat gacaaggagt tgaatggttt
    18121 tgatgaagat gatttttttc atgctaaaac ctccttttcc ttagagacac tcccataaag
    18181 agatgtgttt gtcgtaaaag tctggatagt tttctcttag gtagtcagct gtcatataat
    18241 aaaaagtaga atggcaagca gtgatgattg gcataagaga tgaaaaacgc tgagaactaa
    18301 tgaaggctaa gagaacaaaa aatagaaaat caatggtgag aacccctaaa tagtagaagc
    18361 gaatcttttt attgatttga ggataaatct cagcgaattg atgtttgagt cggacgatca
    18421 agagaaatag caggagtcct tgagcaagga agaaaattaa accagagagc aagagccact
    18481 caaaagatgt ctcgagtcta atgataaaaa atgcaaatag tagcaaatca ataactgcaa
    18541 ttgctataaa atggattcta tagagttttt tcatgacaag acctccctct tttatctaac
    18601 ttcattctac tccaaaagaa tgggagttac aactaaaatg ataaaaatag cagaagggag
    18661 attctcttaa gttggctagt attctttatt tgagtttcct tctattatct aacttcttca
    18721 tcattccaga caaataaagc tccgattgca ttgaggatat aaaagatgta tttaccgata
    18781 ttggcgaagt ttccttgaat accagctttt gtcagctgaa cgaaattgta aatcaaccaa
    18841 aagccccact gagttgttag ttttaatgca ttcaaagcat tggcaatgag ggatagtgca
    18901 aaggcaatag ttgttacgta ggcaaggaga ttcatcttgc cctcatatcc gatatagttg
    18961 gtcacaaagg caaagaggaa ggcgatgatg gaaatgatga tgaccgtcaa ttttacctgt
    19021 ttttggctca tttggttggg tctgccttct tgcgaagctt tccatttctt tatagcaaag
    19081 gtataaatga ggaaggtgac gggataggta atgatggccg ccttatttcc aaggatataa
    19141 tcaatagcac cggacaaaat ggtattaaca atactaaagt aatttcccca tttgcttaat
    19201 ttccccgtga aacgagtgga caacatggaa atcccaacgt tggttacgga aatcaatcca
    19261 aagggtacaa gagctgtcca tggtccccag tctacaaatt tatcgaggcg tgagttgagg
    19321 taaccagatg caatcgcaat cccaacgacc aaagcaaccc cgaagaggtc aaaccattta
    19381 gatgtagcaa aaatttttag tgattttttc ataggttaaa ctacctttct tttttttttc
    19441 aaatattcta ccaccaaatg aaagtaaaat aaaatgatag aaataaaacc ctgaaaataa
    19501 aactttcagg gttggtaggg gacttgagaa attagttgat tttctcgaca tagagttgtt
    19561 ttgcaatgtc catattcact tgtaaatcct cgtcgttact gaggatagtg aaggtattgc
    19621 tgaagccatc aaactgcttg acttggagct ggtcaccgat gtgaagtgag tgcttgtcca
    19681 gataatggag aatgtcaaaa ttatcgtgca cccgagtcag gcggtaggcg ccagcttcct
    19741 tgatatcagc tagtgggagg ttattgattt caacgaggag ttctcccttg gcaggaatag
    19801 ttcctccgtg ggggcaggtt ttagggaaac ctagcagttt atctagtctt tccacgaaca
    19861 ggtcagagac agtgtgttcc aagacctcag cttcctcgtg aatctggtca cttgtatagt
    19921 ctaaatgatg aactagaaaa acttcaatca agcggtgctt acgatagagc tcagagacca
    19981 gtttgagacc gaggtcagtc agtagatagc cacattcctt gtcctttagg atgagatttt
    20041 cacttttcat tcgtttgatc atttcagtta cggcaggggg agagacttgc atgcgagccg
    20101 caatttcctt gttggtaatc ttatgcaggt ctatgccaat ttcataaata cattttagat
    20161 agtcttcttt gtttggggtc attcgtttcc tcttgtctaa tatctttatc tagtatatca
    20221 aaaaaagcta gatttctcta gctgtgactt tttatgttat acttattgca agagaaaaaa
    20281 cgaggagatg gatatgacga aaatagctct tctttcagat attcatggaa ataccaccgc
    20341 cttggaggct gttttggcag atgctcagca gctaggagtg gatgaatact ggcttttggg
    20401 agatattctc atgccaggga caggacgtag aaggattttg gacttgttgg atcaactacc
    20461 gattacggct agagttttgg gaaactggga agacagtctt tggcatggtg tccgtaagga
    20521 attggacagt actcgcccca gtcaacgcta tctcttgcgc cagtgccagt atgttttaga
    20581 ggaaatttcc ctagaagaaa ttgaagtgct ccacaatcaa cctcttcaaa ttcatcgtca
    20641 gtttggggat ttgacggtgg gaattagcca tcatctgcct gataagaact gggggcgaga
    20701 gttgattcat actggcaaac aagaggagtt tgaccgcttg gtgactcatc ctccctgtga
    20761 tattgctgtt tatggacata ttcaccagca gttgcttcgt tacgggactg gtgggcaatt
    20821 gattgtcaat ccgggttcga ttggccaacc tttctttttg gacgctcagc tacggaagga
    20881 cttgcgggcc cagtatatga ttttggagtt tgatgacaag ggcctggtag atatggactt
    20941 ccgacgggta gattacgatg tggcagctga attgcagctg gctaaagacc tgagacttcc
    21001 ctattttgag gtttactatg aaagtctggt caatgggatc caccatactc atcatcagga
    21061 atttttgaga gaattggctc agaaagaggg ctgcgatcgg gagttagacg actggttgaa
    21121 aagtggtaac gattgacatt acaactaaaa agggtataat aatagaaaaa gaagagtaga
    21181 ggcagggtag cctcgtaaaa aggagaagag gatgcaaatt ccaagtagat ttaccattgc
    21241 gactcatatg ctgataatca ttgccctcga ggggaaggaa agcaaggtga ccagtgattt
    21301 tctggctgct agtgtcgggg tcaatcctgt cattatcaga aagatcttgt cccagttgaa
    21361 gaaggcagag ctgatttcag tagcgcgtgg aacgggcgga acagagattg tcaaggacct
    21421 taaggatatt agtcttttag atgtttatca ggcggtcgaa tgtcttggta agacaggtca
    21481 actcttcagt ttccatgaca atccgaatcc aaattgccct gtaggagctc atattcatga
    21541 tgttttggat caaaaattgg agagaattca gttgactatg gaggcagaac ttggtcaaac
    21601 cagtctagaa aaagtcgtgg ccgatgcaga gagtcagatg aaggattaag agggataaat
    21661 gccctctttt ttctttggat aattatgata aaatgtagct attaaaggag taagaaatgt
    21721 atctcgttat aggaattgta ttagccttta tagtgtcatt ttggaaggat aatagaagtt
    21781 tgtggaatcc agtattattt ttattatccc tcatttcaag ttatttttat ctatcctacc
    21841 ttttttataa aaatggatac gagaatgttc agttagcttt ttatgtattt gcctttgtct
    21901 tacttccttt tttgcttttt ctaagtggca tctttttaat ctataatggg gtcattttgt
    21961 tgaagcgaga gggacgttcc aagccccatt atctctcaat gctctttgat ttttattgac
    22021 tattagattc tatttaaaaa gacttagaat gcacatgaaa gtatgtattc taggtctttt
    22081 gttttgtata catttaattg tcaagagtat tatctatttt tccatgcctg gtaacgagta
    22141 tcaatcaata actgggtaag gcgacccaag gtttctcttt cttctggagt gagggattga
    22201 gcttggacaa agagatgatg aatgtattca agcgccttta aggaagccaa gtcattgata
    22261 gagctaatac cagcctcaag aatagtgcca aagaagaggt cgaagtagag ctgaagttct
    22321 tcgtcaggga ctgtggccac ccattcttta aaggttgtgt ctacttgctg gctatcactg
    22381 ttggtcttat ccagttggac gaagtgcttg tcctcaatct gccaactaaa ggtatcgtgc
    22441 tgggcgatgc cacccagggc agtactctga acgatgattt ggtgagcagg aatttccagc
    22501 atcataccga taatggaacc ttgtggaatg aatatcttgc ttctatccat tatcctttga
    22561 taacccgcag tctgtgtcaa ttcttgatgg agaccaggtg catcaaatgt ataaactgct
    22621 gtgatctgat tttgcaaact ttgctcaatt tggctagcag catagatagc gagatttcct
    22681 cccttggaat gcccagctag aataaccttt tgcttaggat gatgggcaaa aaagttcttt
    22741 aaatagcgaa gggcgtgctt ttgagcagga atttccttca tataggtcag gtggaaatct
    22801 tccttccagc caatgatact gtcatctgtc ccacgaaaga caatcagata ggtatcgagg
    22861 ctgacacgat aagtcatagc cgcaaattgc ttttgcagtt cagggtcgat gtcgttgata
    22921 aaatgggaga gtttgcaatt tttgaagcgc ttgtgttgag ccaattcatc taataattga
    22981 aggcgatttt tgctagtaag catggtggga tctcttggaa cctgaggtgc tagatctaaa
    23041 agacgttgag gaagtgtgga gaccagatta tcaaaggaga ggtaggtgat ttctattaag
    23101 gttagaatgt ctaactcatt caagggaagg tcgtaataag aatcatatgc gacatctttc
    23161 agatagtcaa aaatattggc cataagagct cctttctttt tatttatctt agcaaatttc
    23221 cctcaaatta gctagtagca ttgcctgttt gtactggcta aaaacaggct atttcaaatt
    23281 cagtttcaga ccatctggca tggaaaaatc tgttataata atagaaaagg agaagcgcat
    23341 gcacaagatt ttattaatag aagatgatca ggtcattcgt caacaggtcg ggaaaatgct
    23401 ctctgaatgg ggatttgaag tggtcctggt agaagacttt atggaagttt tgagtctatt
    23461 tgttcagtcg gaacctcatc tggtcctcat ggatattggt ttgcccttgt ttaatggtta
    23521 tcactggtgt caggaaatcc gcaagatttc caaggtacct atcatgtttc tttcttcgag
    23581 agaccaggct atggatattg tcatggcaat caatatgggg gcggatgact ttgtgaccaa
    23641 gccttttgac cagcaggttc ttttagctaa ggttcagggc ttgttgcgtc gttcctatga
    23701 gtttgggcgt gatgagagtt tgctggaata tgctggtgtt atcctcaata ccaaatccat
    23761 ggatttacat tatcaagggc aagtcttgaa tttgaccaag aatgaattcc agattttacg
    23821 cgtgttattt gagcatgcag gcaacatcgt agcacgtgac gacctgatgc gggaactttg
    23881 gaacagtgac tttttcattg atgataatac cctctctgtc aatgtggctc gtttgcgtaa
    23941 aaagttggag gagcagggat tggtaggatt tatcgagacc aagaaaggaa tagggtacgg
    24001 attgaagcat gcttgattgg aaacaatttt ttctagccta tctgcgctcc cgtagtcgtc
    24061 tttttatcta tctgctttct ttggcatttc ttgtcttact ctttcagttt ttatttgcca
    24121 gtctaggaat ttacttcctc tactttttct tcttgtgttg ctttgtaacc atcttatttt
    24181 tcacttggga catattggtg gaaacgcagg tctatcgcca ggaacttctc tatggagaga
    24241 gggaagccaa gtctcctttg gaaatagctt tagcagaaaa attagaagcg cgtgagatgg
    24301 aactctatca gcagaggtca aaagcagaaa gaaaactgac ggatttgctg gattactata
    24361 ccttgtgggt ccatcagata aagaccccca ttgcagccag tcaactctta gttgcagaag
    24421 tggtcgaccg ccaactgaag cagcagctag aacaggaaat tttcaaaatc gactcctata
    24481 ccaacctagt tttacagtac ctgcgtttag aaagtttcca tgatgatttg gtcttaaagc
    24541 aggttcaaat tgaggacttg gtcaaggaaa taattcgtaa atatgctctt ttctttattc
    24601 aaaaaggctt aaatgtcaat ctacatgacc ttgataaaga aatcgtgacg gataaaaagt
    24661 ggctgctagt ggttattgag caaatcatct caaacagtct caagtacacc aaggaaggtg
    24721 gtctggagat ttatatggat gaccaagagc tttgtatcaa agatacggga atcgggataa
    24781 aaaacagtga tgtcctccga gtatttgaac gtggcttttc aggatacaat ggccgtttga
    24841 cccagcagtc ctctggactt ggcttttatc tatctaagaa aatttctgaa gaactggggc
    24901 accagattcg tatcgagtct gaggtcggaa aaggaacgac agtgcggatt cagtttgctc
    24961 aagtgaactt agtccttgag taaaaataga tgagaaaaag cctccagatt ggaggctttt
    25021 atgctagaca cttatttctc aacgcgtgat ttgttggcga tatcagctag gatagcttga
    25081 acaaaattat caactgagac agtttgtgtt tctttttggc cgtagcgacg aacgttgact
    25141 gtttcgtctt ccatttcttt gtctccaaca attaattggt aaggaatctt gctggtttgt
    25201 gaagcacgga tcttgaactg cattttttca ttgcgctcat ctacgtctgc acggacaccg
    25261 cggtcacgga gtttcttggc cacttcccaa gcgtagtcca cgtgtttttc gttagatact
    25321 gggatgaggg ttacttggtg tggtgccagc catgttggga aggccccctt gtagttctca
    25381 atcaagatag ctgtgaagcg ttccatagtt gagataaccc cacggtggat catgactgga
    25441 cggtgatctt cgccatcagc tccgatgtat ttgaggtcga agcgttctgg caacaagaaa
    25501 tcaagttgga tagtagaaag ggtttcttct tttccaaggg cagttttaat ctggatatcc
    25561 aattttggtc cgtagaaggc tgcttcacct tcggcttcaa agtagtccac gcccatttca
    25621 tcaagagctg cacgaagcat agtttgggca ttttcccaca tctcatcgtt atcaaagtac
    25681 ttatgagtat cttgagggtc acgaagagag aggcggaagc ggtagtcagt caagttgaag
    25741 tcttcataaa catcgataat caactgaagg gcacgttgga attcttcttg gatttgttct
    25801 ggagtaacga ataggtgacc gtcgttgagt gacatttcac gtacacgttg aaggccagtg
    25861 agggcaccag atttttcgta acggtgcatc ataccgattt cagcgatacg gattggcaat
    25921 tcacggtaag agtgaacatg gtgtttaaaa acttggatgt ggtgcggaca gttcattgga
    25981 cgaaggacaa attcttcccc gtcacccatg tccatggttg ggaacatgtc ttcttggtaa
    26041 tgatcccagt gaccagaagt cttgtaaagc tcaacagaag caagtggtgg agtgtagacg
    26101 tgttggtagc cagaagccaa ctctttgttt acgatgtagc gttccaattc acgacggata
    26161 gtcgcaccat ttggcaacca gaatggcaaa ccttgtccca cttcttgtga aatcataaag
    26221 aggtcaagct ctttaccaag tttacggtgg tcacgttcct tagcttcttc acgcatttga
    26281 aggtagtttt tcaagtcttt cttgtcaaac caagctgtac cgtagatacg ttgcatcata
    26341 gcgttgtcgc tgtttccacg ccagtacgca ccagctacat ggagaaggtg gaagatttgg
    26401 atacgacctg ttgatggaac gtgaggtcca cggcagaggt ctacatattc accctgacga
    26461 tagatagtca aaccgccttc gtcttctgag tgttcttcaa tcaattccaa cttgtaaggg
    26521 tcatttttga agatttcacg tgcctcgtct ttagtcactt cttcacgaat agatgggaag
    26581 ttttctttga cgattttttg catttcttct tcgatacgag gaaggtcttc gttagagatt
    26641 tgaccagctg tgttgtcagt atcgtagtag aaaccatctt cgatggctgg accaactccc
    26701 aagtgaatgt ctgggaaaag acgacgagct gcttgggcga acaagtgagc tgctgagtga
    26761 cgcaagattg gaagggcatc ttcgtgatca ggtgtcacaa tttcgatgct tccatcttca
    26821 gtgatagcgc gagtagtgtc gatgagtttg ccgttgaatt taccagccaa ggctttttta
    26881 gctagggaat tgctgataga ttgggcaatt tcaaaagttg ttacgccaga ttcgaattca
    26941 cgaacagcgc catctgggaa agtaatgtta atcatgatgt tctccttttt tattatttca
    27001 tagtttctaa gcattgattt tttatcagca gactacttcc gagtcctaac gttcaattgc
    27061 ttttctttat cagagaggaa gttttccatt tccgtcagaa tgtctatatc caaacgttga
    27121 gaaagttcta atagccacca gatattttct gaaagttttt gttccagtgt gtagggtgtt
    27181 tcatcatagt agcgtccttg ctttgtcatc accagtcatt ggaaatttcc aatatcatta
    27241 gataaagcca agaggtcttc ttctaccgtc cacttgaaat catgatgctt aacttccagt
    27301 tcgtgataag cttgtcggat tgcccaactg cgctccacta attcttgcaa ctccataatt
    27361 agtatcctaa taaaaaaatg cgacatcctc gaaaggacgt cgcaacgtgg ttccaccttc
    27421 atttatgtac ctcaaaagag agggacacct ctgatcggct ctaacgtggc caccgtttta
    27481 tgttttcata aaaaactcaa gagtagtatc agtttagact tcctatgcgt ttccagcaac
    27541 cacgcactct ctagtagaaa gggactaagt gacttgtctc tatggattat tatagcatga
    27601 ttgtgagatt tttcaaggat tttgtgaaag ttttttgttt ttcatttttt gtagaatatg
    27661 cttgatgcta ataccagaat ccatgccgac cagagcaact gtttcataga ttagcaagta
    27721 aattagtata aactcaccaa aggttatgat agtaaagtaa aagagcagtc cgttaccaac
    27781 taaccatcaa agtgagaaac ggaaacggta gctatagagt aacgatataa tcaagattac
    27841 cagaggtgta aagaagagaa gattgtttat atagagttcc ttgagtgtaa gggggtcaga
    27901 aacaactagt aagaacaact cataaagtgg ccaataaaag aataatatga ccagatagta
    27961 ggggagatgg gaatagaatc tacgcatgat aatcacctcg ttttaaaaaa acagacaact
    28021 tagatctctt gctatgatta ttatagcatg tttaaaacaa gaaagtaaat ttgttgacaa
    28081 agaagatttt tcttttcact agaatcatag ttagttttct tggtagaaat attttgataa
    28141 taattaagca agactattga cattgattat taagagtgtc acaattattg taactaaaat
    28201 aattacaaca agagaggtat aaaaaatgaa actagcagtt attgcagcaa atggacaagc
    28261 ggggaaagca attgttgaag aagcagtcaa acgaggacat gaagtaacgg ctattgttcg
    28321 ttctgaaaac aaaagtcaag ctgagtctat tattaaaaaa gatttatttg agttgacaaa
    28381 agatgatctt actggatttg atgctgttat ttcagcgttt ggtgcttata caccagatac
    28441 gctaccgctc cacagcaaat caatcgaact atttaatcag cttcttgctg gaacacagac
    28501 acgttttcta gttgtagggg gagcaggaag tctttatata gatgaaacaa aaacgacacg
    28561 acttttagat acgtctgatt ttcctgaaga atttaagtca ctagcaaaag atcaagcgga
    28621 tgaattggat cttcttcgta caaaaaataa tctcaactgg acttttgtca gtcctgctgt
    28681 agattttatt cccgatggtg aaaaaacagg aaactacatt ctagcaggag aaatttttac
    28741 tactaatgaa aaaggaataa gccagattag ttatgcagat tatgctattg gattggtaga
    28801 tgaacttgaa aaaggtcatc atataaaaga acgtatctct ttacttgaaa aataataact
    28861 atgagagtat taatgtttga cagaaaattt aaatcttggt aaaataggga ttgtcgtaaa
    28921 gacaaataac ttcttcttgg ttacaggcat gccaacctgt cactcggatg aagccaaata
    28981 aaaaggagaa acatcatggc aatctcaaaa gagaaaaaaa atgaaatcat tgcacaatat
    29041 gcacgtcacg aaggtgatac aggttcagta gaggttcaag ttgctgtcct tacttgggaa
    29101 atcaaccacc ttaacgaaca catcaaacaa cacaaaaaag accacgctac ttaccgtgga
    29161 ttgatgaaaa aaatcggtcg ccgtcgtaac ttgcttgcat acttgcgtaa aaacgacgtt
    29221 aaccgttacc gtgagttgat caactctcta ggacttcgtc gctaattcaa gatacaaatg
    29281 ctgtcaaaag cacaaagcaa aaataggaaa attgacgaag aaacttcagt ttctaggaga
    29341 ttttatcttt tttgccaagt gcttaggccg tgttcaattg agcatatctt gataatgaag
    29401 ctactctaaa ttgggtagct tttttgcgtt gtcaccttac ctcgatatac tcaagtataa
    29461 tctttggtta cggttcctag cactgtaagg taaaataaac cagcttttct cccttcgggg
    29521 agattttttt tgtttcactg aaagtttcgt acgatcttcg cctcgtcgcc tagtctataa
    29581 acgatttatc tagcaagaat catgatgcta agggcgtcaa aaatccgtat gaaaataggg
    29641 aaatgacaca gtgttcgatg agcacaagga gtttcatctt tttcactagg attttagccc
    29701 gggctcaaat cagctctctg acttcagagg gcttttttat tgaggtttta tcggtaacaa
    29761 ttttggagac tacaaaaacc tcaaacggta tcagctaatt acaccataaa ggtgcgcagt
    29821 tactcggctt ttcaagccga gtagctgtat gcaagccccc tagaagagcc cactctttac
    29881 aaagtaaagt atagtatgtt atactttaca tggaagtagt cactgaattc cagttagaaa
    29941 ttactttgta actacgtttt gaggaggagt aaaatgcttt cctacgttcg acattaccca
    30001 ctagcgatag ctaaattaat gtgtctgtgc tctcctaaaa tctgctgatt tattactgac
    30061 taatacagga ggtttttttt atgggacaga caatcatatc tgctattggt gtttatattt
    30121 ccaccagtat cgattattta attattttaa ttattttatt tgcacagcta tcacagaata
    30181 aacagaaatg gcatatttat gcggggcaat atctaggcac aggcttactt gtaggggcga
    30241 gtttagttgc tgcttatgtc gttaatttcg tgcctgaaga atggatggtt ggattgcttg
    30301 gtttaatccc tatctattta gggattcgct ttgcaattgt tggagaagat gcggaagaag
    30361 aagaggaaga aattattgaa agattagaac aaagcaaggc aaatcaactg ttttggacag
    30421 ttacattgct gacaattgcg tctggcggag ataatttagg tatctatata ccttattttg
    30481 cttcgttaga ttggtcacag accctcgtgg ccttgcttgt gtttgtaatc ggcataatta
    30541 tcttttgcga gattagtagg gtgttatcct ctattccgtt aatattcgag acaattgaaa
    30601 aatacgagcg aatcattgtg cccttagtat tcattctact tggactatac atcatgtatg
    30661 aaaatggcac gatagagact tttctgatcg tgtagatttt tttgtttcac taggatttta
    30721 gcccgagctc aaatcagctc tctgattttc agagggcttt ttcattgagt ataaatggac
    30781 aatcttaaag ttactctatt gtagagtggc ttttgatttg attaggagat tcttataaat
    30841 atagtaaaat gaaataagaa taggacaaat cgatcaggac agtcaaatcg atttctaaaa
    30901 atgttttaga agtagaggtg tactattcta gttccaatct actatataaa tgaaaacctt
    30961 agctgtttgg ccaaggttca tttcttctat gcaagttctt ctcttttctt atctgggttc
    31021 cagatgaagc ttgcgataaa gctaaagatg atggttagga tagcgaggat aatagcaagg
    31081 atgagggcag ggatgcggat aaaccaccag agtggatttg gttttttatc attgtgccat
    31141 tgtttcgttt cttcgtccag acgttggaat tctgtttgaa tacgattggc gactgtttca
    31201 atgccttcat cattcatttt cttgatatct gagatatcga ttggatttcc aaagttcata
    31261 tcgacacgtt cacggctaat caagcccttc aaagtcatgg gaccggtgta ggtaaccggc
    31321 atgatacgga ccttggccat tttggcaatc agtgctgcgc cccccttgac atcgtttgag
    31381 tggcggctac cacttggaaa catgatgaga gagcggtcac tttttttgag aacgttgata
    31441 ggatatttga tggctgaggc gctgggattt tcacggtcga tgggaaaggc gccacacata
    31501 cgaatccacc aaccaaagat acggttggta aagagttctt tttttgccat aaagatgaac
    31561 tgttttggct tggtcgcaaa ggccatataa acaggatccc accaggtacg gtgaggcgca
    31621 actaaaatat aattttcatc ttgattagga attttatcag tattatgata gtgagcattg
    31681 ccattgatgg accataagag caatacaact aatccacgca aataagtata aaacatgcga
    31741 tctccttcga ttgttttctt gttattatta taccttatca aaggagggct ggcaaacttt
    31801 tcccttgact agatacatat ttaggatgaa attagaattc tgttaaaaaa aatgatataa
    31861 tagaatttat ggataaaaat aagattatgg gattaaccca aagagaagtc aaggaaagac
    31921 aggctgaggg tttggtcaat gactttaccg catcagccag taccagcact tggcaaatcg
    31981 ttaaacgaaa tgtctttacc ctttttaacg ctttgaactt tgccattgct ttggctcttg
    32041 cctttgtgca ggcttggagc aatctggtct tctttgctgt tatctgcttt aacgcttttt
    32101 ctgggattgt gaccgagcta cgagccaaac acatggtgga caagctcaat ctcatgacca
    32161 aggaaaaggt caaaaccatc cgtgatggtc aggaagttgc tcttaatcct gaagaattag
    32221 tgctaggaga tgtcattcgt ttgtctgcag gagagcagat tcctagtgat gccttggttt
    32281 tggaaggctt tgcggaagtc aatgaagcca tgttaacggg agaaagtgat ttggtgcaaa
    32341 aggaagttga cggcttactt ttgtcaggaa gtttcctagc cagtgggtca gttttatctc
    32401 aagttcacca tgtcggagca gacaactatg ctgccaaact catgcttgag gctaagaccg
    32461 ttaaacccat caactcccgt atcatgaaat cgctggacaa gttggctggt tttactggga
    32521 agattatcat tccctttggt ctggctctct tgctggaagc cttgctttta aaaggcctgc
    32581 ctctcaagtc atccgttgta aactcgtcga cagctctttt gggaatgttg cctaagggaa
    32641 ttgccctttt gaccattact tcgcttttga ctgcagtgat taagctaggt ttgaaaaagg
    32701 tcttggtgca ggagatgtac tctgttgaga ccttggcgcg cgtggatatg ctctgtctgg
    32761 acaagacggg caccatcacc caaggaaaga tgcaggtgga ggctgttctt ccactgacgg
    32821 aaacttacgg tgaagaggct attgccagca tcttgactag ctacatggcc catagtgagg
    32881 ataaaaatcc aacagctcaa gccattcgcc agagattcca aggtcaagta gtctatccta
    32941 tgctctctaa tcttcccttc tccagcgacc gcaagtgggg agccatggag ttagaaggtt
    33001 tgggaatagt tttcttaggg gcacctgaga tgttgctgga ttctgaggtc ccagaagcca
    33061 gagaggcctt ggagagaggt tcacgtgtct tggtcttagc tctcagtcag gagaaattag
    33121 accatcacaa accacagaaa ccatctgata ttcaggctct agccttgctg gaaatcttgg
    33181 atcctattcg agagggagcg gcagagacgc tgaactatct ccgttctcag gaagtggggc
    33241 tcaagattat ctctggtgac aatccagtta cggtatccag cattgcccag aaggctggtt
    33301 ttgcggacta tcacagctat gtcgattgtt caaaaatcac ggatgaggaa ttgattgctc
    33361 tggcggagga gacagcgatt ttcggacgtg tttcccctca tcaaaagaaa ctcattatcc
    33421 aaacgctgaa aaaagcgggt catacaacgg ctatgacagg ggacggggtt aatgatatct
    33481 tggcccttcg tgaggcggat tgttctatcg taatggcgga gggagacccg gcaacccgtc
    33541 agattgccaa cctggttctc ttgaactcag actttaatga tgttcctgag attctttttg
    33601 aaggtcgtcg tgtggtcaat aacattgccc acatcgctcc gattttcttg ataaagacca
    33661 tctactcatt cttgctcgca gttatctgta tcgccagtgc tttactaggt cggtctgaat
    33721 ggatcttgat tttccccttc attccaatcc agattaccat gattgaccag tttgtggaag
    33781 gtttcccacc attcgttctg acttttgagc gaaatatcaa acctgttgag ccaaatttcc
    33841 tcagaaaatc catgcttcgt gccctaccaa gcgctctcat ggtcgtgttt agcgtcctgt
    33901 ttgtgaaaat atttgggagt agccaaggtt ggtctgagtt agaaatctca actctcctct
    33961 attatctctt ggggtcaatt ggtttcttat ccgtatttag agcctgcatg ccatttaccc
    34021 tatggcgagt cctattgatt gtctggtcag taggaggctt cctagccaca gctctcttcc
    34081 caagaattca aaaactgctt gaaattgcaa ccttaacagg acaaacatta cctgtttatg
    34141 gtgtcatgat gttggtcttt accgtgattt tcatcctgac cagtcgctat caagccagaa
    34201 aataaagaaa ggctgcaatc tgtggattgc agttttttta ggtacaagat tgccagtcga
    34261 aatgtggtat aataagaggt aatagagttt tggaaagtga gagaagatga tttcaaagag
    34321 attagaattg gtagcttcct ttgtgtcaca gggggctatt ttactagatg tgggaagtga
    34381 ccatgcttat ctgcctatcg agttggttga gagaggccaa atcaaaagcg ctattgcagg
    34441 tgaggtggtg gaaggtccct atcagtctgc ggttaaaaat gttgaggctc acggcctaaa
    34501 ggagaaaatc caagtccgtt tagccaatgg cttggcagct tttgaagaga ctgaccaagt
    34561 gtctgtcatt accattgctg gcatgggtgg tcgtttgatt gctaggattt tagaagaagg
    34621 tttggggaag ttagctaatg tagagcgttt gatcctccag cccaataatc gtgaagacga
    34681 cttgcgtatc tggctacagg atcatggatt ccagattgta gcagaaagca tcttagaaga
    34741 agctggaaag ttttatgaga ttttggtggt ggaagcagga caaatgaagc tatcagccag
    34801 tgatgttcgc tttggtccct tcttgtccaa agaagtcagt ccagtatttg tccaaaaatg
    34861 gcaaaaagaa gctgagaagc tagagttcgc cctcggacaa atcccagaaa aaaatctgga
    34921 agaacgtcaa gttctagtag ataagattca agctatcaag gaggtgctcc atgttagcaa
    34981 gtgaagtgat ccaagcttat gaagcctttt gccctcagga attttctatg gagggagaca
    35041 gccgtggtct gcaaattggc actttagaca agggtatcca aagggtcatg gttgccctcg
    35101 atattcgtga agagacagtg gcagaggcca ttgaaaaggg tgtggacttg attatcgtca
    35161 agcacgcgcc tatctttcgt cctatcaagg acttgcttgc cagccgtccg caaaatcaga
    35221 tttacatcga cctgattaag catgatatcg cagtttatgt cagccatacc aatattgata
    35281 tcgttgaaaa tgggctcaat gactggtttt gtcagatgct aggaatcgag gagacgactt
    35341 atctgcagga aacaggtcca gaacgtggaa ttggacgtat tgggaatatt cagcctcaga
    35401 cattttggga attggcccaa caggtcaagc aagtctttga cctagatagc cttcgaatgg
    35461 tgcattatca agaggatgat ttgcagaagc ctatttcaag agtagcaatt tgtggtggaa
    35521 gcggacagtc tttctataag gatgctttgg caaagggggc agatgtctat atcactggtg
    35581 atatctacta ccatactgct caggatatgc tgtctgatgg cttgttagca ttggatccag
    35641 gtcactatat cgaagtgctt tttgtggaaa aaatcgcaac actccttact caatggaaag
    35701 agaaaaaagg ctgggaacta gagattttac ctagtcaagc atcgaccaat cctttccgtc
    35761 atatctagtt agaaagtgaa gacaatgaaa aaagttgcca ttattggagc aggaattgtg
    35821 ggagcaacag ctgcctacta cctctcgaga gaaagtgacc tagaggtgac cgtttttgac
    35881 catgagcaag gtcaagccac caaggctgca gcaggaatta tcagtccttg gttttccaaa
    35941 cgccgtaata aagcctggta caagatggcg cgcttggggg ctgattttta tgtggattta
    36001 ttagctgatt tagagaaatc aggacaaaaa atcgactttt accagcgttc gggagtcttt
    36061 ctcttgaaaa aggatgaatc caagttggaa gaactttatc aactggccct ccagcgcaga
    36121 gaagaatctc ccttgatagg gcaattagcc attctggacc aagcctcagc taatgaatta
    36181 ttccctggtt tgcagggatt tgaccgcctg ctctatgctt ctggtggagc gagagtagat
    36241 ggccacctct tagtgactcg tttgctggaa gccagtcagg tcaagctggt caaagaaaaa
    36301 gtgactctga caccgttagc atcaggctac cagattggtg aagaggagtt tgagcaggtt
    36361 attttggcga cgggagcttg gttgggggac atgttagaac ccttgggtta tgaagtagat
    36421 gttcgtcctc aaaaaggaca actacgagat tatcagctgg tccaagacat ggaagcttac
    36481 cctgttgtca tgccagaagg ggagtgggat ttgattcctt ttgcaggtgg gaaattgtcc
    36541 ttaggcgcta cccacgaaaa tgacatggga tttgatttga cggtagatga aaccttgctc
    36601 caacaaatgg aggaggccac cttgactcac tatctgattt tggctgaagc tacttcaaaa
    36661 tctgagcgtg tgggaatccg tgcctatacc agtgatttct ctcccttctt tgggcaggtg
    36721 ccagaattgg caggtgtcta tgcagccagt ggactaggtt catcaggcct cacaactggt
    36781 cctatcattg gttaccatct agcccaactg atccaagaca aggagttgac cttggaccct
    36841 ctaaattacc caattgaaaa ctatgtcaaa cgagtaaaaa gcgaataata ctcaatgaaa
    36901 atcaaagaac aaactaggta actagccgcg ggttactcaa agcactg
//
LOCUS       DBEROU010000020        34119 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.020, whole genome shotgun sequence.
ACCESSION   DBEROU010000020 DBEROU010000000
VERSION     DBEROU010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 34119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34119
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.020"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..1144)
                     /locus_tag="ACL8CL_001030"
                     /pseudo
     CDS             complement(1..1144)
                     /locus_tag="ACL8CL_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285359.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1126..1433
                     /locus_tag="ACL8CL_001031"
                     /pseudo
     CDS             1126..1433
                     /locus_tag="ACL8CL_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1588..1911)
                     /gene="tnpB"
                     /locus_tag="ACL8CL_001032"
     CDS             complement(1588..1911)
                     /gene="tnpB"
                     /locus_tag="ACL8CL_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS1330292.1"
                     /translation="MTIHLSSLGQVYLVCGKTGMRQGIDSLAYLVKTHFELNSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPKI"
     gene            complement(1892..2077)
                     /locus_tag="ACL8CL_001033"
     CDS             complement(1892..2077)
                     /locus_tag="ACL8CL_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330293.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MIEQLLDKVLLYDNSSI"
     gene            complement(2381..3943)
                     /gene="guaA"
                     /locus_tag="ACL8CL_001034"
     CDS             complement(2381..3943)
                     /gene="guaA"
                     /locus_tag="ACL8CL_001034"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HGS1330294.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRTGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKADWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4085..4783
                     /locus_tag="ACL8CL_001035"
     CDS             4085..4783
                     /locus_tag="ACL8CL_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1330295.1"
                     /translation="MLPAYMKIHDQIKKDIDEHRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKYGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4826..5722)
                     /locus_tag="ACL8CL_001036"
     CDS             complement(4826..5722)
                     /locus_tag="ACL8CL_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS1330296.1"
                     /translation="MSNSLKGTLLTVVAGIAWGLSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMLLAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQADIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     IGMAMILFAVTLISLKDLFLEK"
     gene            complement(5731..6666)
                     /locus_tag="ACL8CL_001037"
     CDS             complement(5731..6666)
                     /locus_tag="ACL8CL_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HGS1330297.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6663..7388)
                     /locus_tag="ACL8CL_001038"
     CDS             complement(6663..7388)
                     /locus_tag="ACL8CL_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HGS1330298.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDRASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLGKDRNNVELWFEEV"
     gene            complement(7472..8107)
                     /locus_tag="ACL8CL_001039"
     CDS             complement(7472..8107)
                     /locus_tag="ACL8CL_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HGS1330299.1"
                     /translation="MAVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EALNSLEAIIQSVARDYPLTEIKLLSILPVNEGEKYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKALKDYLY"
     gene            complement(8109..8936)
                     /locus_tag="ACL8CL_001040"
     CDS             complement(8109..8936)
                     /locus_tag="ACL8CL_001040"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1330300.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     DQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(8958..9293)
                     /locus_tag="ACL8CL_001041"
     CDS             complement(8958..9293)
                     /locus_tag="ACL8CL_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-gamma-glutamate biosynthesis protein"
                     /protein_id="HGS1330301.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPTWVNRTPKGTFSPEGYPLYHYQTYILEDFIEDGSHRDQLDEATKERIDTAYKEM
                     NEHVGLKWY"
     gene            complement(9294..9884)
                     /locus_tag="ACL8CL_001042"
     CDS             complement(9294..9884)
                     /locus_tag="ACL8CL_001042"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HGS1330302.1"
                     /translation="MGDFEGTINKDHYLAGYLLFNAPAEVMDAIKEAGYHVLDLAHNH
                     ILDSQIEGVISTADIIEKAGITPIGVYTHEPRDQALLVIKEVNGIKVALLAYSYGFNG
                     IEQYISQEDYNRYLSDLNEDKMKVEIERAEKEADITIIMLQMGVEYRLELTEEQKALY
                     HKMIDWGADIIFGGHPHVVEPSETVEKDGDKKLIIY"
     gene            complement(9900..10022)
                     /locus_tag="ACL8CL_001043"
     CDS             complement(9900..10022)
                     /locus_tag="ACL8CL_001043"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330303.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQGD"
     gene            10535..11146
                     /gene="def"
                     /locus_tag="ACL8CL_001044"
     CDS             10535..11146
                     /gene="def"
                     /locus_tag="ACL8CL_001044"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HGS1330304.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRAIAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11191..11919
                     /gene="rlmB"
                     /locus_tag="ACL8CL_001045"
     CDS             11191..11919
                     /gene="rlmB"
                     /locus_tag="ACL8CL_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HGS1330305.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(11958..12869)
                     /gene="trxB"
                     /locus_tag="ACL8CL_001046"
     CDS             complement(11958..12869)
                     /gene="trxB"
                     /locus_tag="ACL8CL_001046"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HGS1330306.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQAFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDSHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12935..13159)
                     /locus_tag="ACL8CL_001047"
     CDS             complement(12935..13159)
                     /locus_tag="ACL8CL_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HGS1330307.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTAKARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13237..13980)
                     /locus_tag="ACL8CL_001048"
     CDS             complement(13237..13980)
                     /locus_tag="ACL8CL_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1330308.1"
                     /translation="MIKISNLSKSFSGQTVLDHLDLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(13980..14780)
                     /locus_tag="ACL8CL_001049"
     CDS             complement(13980..14780)
                     /locus_tag="ACL8CL_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1330309.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14938..15294)
                     /locus_tag="ACL8CL_001050"
     CDS             complement(14938..15294)
                     /locus_tag="ACL8CL_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HGS1330310.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15307..15819)
                     /locus_tag="ACL8CL_001051"
     CDS             complement(15307..15819)
                     /locus_tag="ACL8CL_001051"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HGS1330311.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDINK"
     gene            complement(15819..16313)
                     /locus_tag="ACL8CL_001052"
     CDS             complement(15819..16313)
                     /locus_tag="ACL8CL_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HGS1330312.1"
                     /translation="MEIRLAFPNEVGAIMQVIEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16448..16894
                     /locus_tag="ACL8CL_001053"
     CDS             16448..16894
                     /locus_tag="ACL8CL_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HGS1330313.1"
                     /translation="MKTTFSYPKWAEIPNINLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16891..17538
                     /locus_tag="ACL8CL_001054"
     CDS             16891..17538
                     /locus_tag="ACL8CL_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HGS1330314.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
     gene            complement(17559..18140)
                     /gene="pdxT"
                     /locus_tag="ACL8CL_001055"
     CDS             complement(17559..18140)
                     /gene="pdxT"
                     /locus_tag="ACL8CL_001055"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HGS1330315.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQNMLLPIREAILSGLPVFGTCAGLILLAKEITSQKESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGKIPMTFIRGPIISSVGEGVEILATVNNQIVA
                     AQEKNMLVSSFHPELTDDVRLHQYFINMCKEKS"
     gene            complement(18141..19016)
                     /gene="pdxS"
                     /locus_tag="ACL8CL_001056"
     CDS             complement(18141..19016)
                     /gene="pdxS"
                     /locus_tag="ACL8CL_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042819 - vitamin B6 biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HGS1330316.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(19168..20547)
                     /gene="nox"
                     /locus_tag="ACL8CL_001057"
     CDS             complement(19168..20547)
                     /gene="nox"
                     /locus_tag="ACL8CL_001057"
                     /EC_number="1.6.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H2O-forming NADH oxidase"
                     /protein_id="HGS1330317.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
     gene            20841..21764
                     /locus_tag="ACL8CL_001058"
     CDS             20841..21764
                     /locus_tag="ACL8CL_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HGS1330318.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHGIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
     gene            21824..22429
                     /locus_tag="ACL8CL_001059"
     CDS             21824..22429
                     /locus_tag="ACL8CL_001059"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HGS1330319.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQAFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            22448..23692
                     /locus_tag="ACL8CL_001060"
     CDS             22448..23692
                     /locus_tag="ACL8CL_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HGS1330320.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRHFKLKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(23809..24066)
                     /locus_tag="ACL8CL_001061"
     CDS             complement(23809..24066)
                     /locus_tag="ACL8CL_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HGS1330321.1"
                     /translation="MDPKKIARINELAKKKKTEGLTLEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(24108..26144)
                     /gene="glyS"
                     /locus_tag="ACL8CL_001062"
     CDS             complement(24108..26144)
                     /gene="glyS"
                     /locus_tag="ACL8CL_001062"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HGS1330322.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKGKRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIQSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGGATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            complement(26356..27273)
                     /gene="glyQ"
                     /locus_tag="ACL8CL_001063"
     CDS             complement(26356..27273)
                     /gene="glyQ"
                     /locus_tag="ACL8CL_001063"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HGS1330323.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATSPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISDQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRAKLLAEDAE"
     gene            complement(27816..28393)
                     /locus_tag="ACL8CL_001064"
                     /pseudo
     CDS             complement(27816..28393)
                     /locus_tag="ACL8CL_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
     gene            complement(28666..29508)
                     /locus_tag="ACL8CL_001065"
     CDS             complement(28666..29508)
                     /locus_tag="ACL8CL_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HGS1330324.1"
                     /translation="MNTYQLNNGVEIPVLGFGTFKAKDGEEAYHAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREEMFVTTKLWNSQQTYEQTRQALEKSIEKLGLDYLD
                     LYLIHWPNPKPLRENDAWKTRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     TIVPAVNQVRLAPGVYQDQVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     IAQIALAWSLAEGFLPLPKSVTTSRIQANLDCFGIELSHEERETLKTIAVQSGAPRVD
                     DVDF"
     gene            complement(29622..31013)
                     /gene="pgdA"
                     /locus_tag="ACL8CL_001066"
     CDS             complement(29622..31013)
                     /gene="pgdA"
                     /locus_tag="ACL8CL_001066"
                     /EC_number="3.5.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050119 - N-acetylglucosamine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-N-acetylglucosamine deacetylase
                     PgdA"
                     /protein_id="HGS1330325.1"
                     /translation="MDKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIELLKKEKDDQLSEGNQKEYFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKSFSDQDLSAWNFDYKDS
                     QIILYPSPVLENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKYQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEASILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            complement(31251..31619)
                     /locus_tag="ACL8CL_001067"
                     /pseudo
     CDS             complement(31251..31619)
                     /locus_tag="ACL8CL_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000603324.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            31840..32817
                     /locus_tag="ACL8CL_001068"
     CDS             31840..32817
                     /locus_tag="ACL8CL_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HGS1330326.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLAG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFEKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQIPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            32814..33896
                     /locus_tag="ACL8CL_001069"
     CDS             32814..33896
                     /locus_tag="ACL8CL_001069"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1330327.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     ILRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKVILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(33960..>34119)
                     /locus_tag="ACL8CL_001070"
     CDS             complement(33960..>34119)
                     /locus_tag="ACL8CL_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGS1330328.1"
                     /translation="WKEIRKRGFKNKAFRTLEDVMNQLQDVIQGLEKEVIKSIVNQRW
                     TRMLFESR"
BASE COUNT        10082 a         7644 c         6177 g        10216 t
ORIGIN      
        1 tctaaaacct ctttgtttac gagagtttcc tcgtttggaa gacattctag aagataggat
       61 agatatttct cactatttag ttgatgacgt ttagctgttt ccaacaaact cataataata
      121 gctcttgctt tagctccttc aaaactttga gaaaacaacc accctcgctt ttttaaactg
      181 agcttaggct aaaagagtcc agtggactct tttactccgt cccataacca atgatttaat
      241 ggcgtgttca gctagattat tggaaaggac cagatgtccg tcttttaaaa tggtcttaaa
      301 ggtttcttca tacttgaggc tgtattcaat tgcccttcct ggttttgaac ctgctaaaac
      361 tgactgacgg cggcaccaag caaagaagtc ttccattaag ggctggacgt ttctgtagtc
      421 gttcatcagc tggcaaagcc tcccagtctc tttccaaggc aaataactgg tcacgatagg
      481 ctaaaccttt agctcctaag gatgatttat ctgcttgctt gggggtcgct tcaaaaaatt
      541 tccttctcac atgcgcccaa catccaacaa gttcagcttc ttccagttga cgataagctt
      601 ctagcggttc gcagtttacc aagcttagcg tcgccttgct cctaaacctt agactgctat
      661 cgcataggca gaactagagg actaaagttc taagttactg ccgcaacata tcacaatgca
      721 tatagccaga ataatttcct aggaattctt gcactaccga accactccga cgctgatcat
      781 ggtggtacag cgtaatccct tgtttctcag ctttcccaga caaaaaagtc caatagtaag
      841 tcagctgact atcactttct aagacccgat aagaggtttc atctgcatga agaagagctt
      901 gttctaacaa cttttctcgt aaaaggttat aaaggggctc caaatagtat tgactcgtct
      961 tgatatgcca attagagatt tccttacgtg tgattggtaa acccatccta gcccaatctt
     1021 cttcttggcg ataattgggt accttcagat taaacttctg atggatggtg tgagcgataa
     1081 tagaagctga gccaaggcta tgcgccaaag gggctttagg aataggagct ttcacaagct
     1141 tatccagatg attatctttt actcgttatg aacaatgcta tatggcataa atcaagtacc
     1201 ttaaagattc cgactaatat tggctttgca tttattcctc catacacacc agagatgaac
     1261 ccattgaaca agtgtggaaa gagattcgta aacgtggatt taagaataaa gcctttcaaa
     1321 ctttggaaga tgtcatgaat caacttcaag atgttataca aggactggag aaggaggtga
     1381 taaagtccat cgttaatcgg agatggacta gaatgctttt tgaaagcaga tgagtataaa
     1441 aagaaagtcc tcatttcaat agaaatcacg actttctgat ggatttatag taaaatgaaa
     1501 taataacagg acaaatcgat caggacagtc aaatcgattt ctaacaatgt tttagaagca
     1561 aaggtgtact attctagttt caatctacta tatttttgga gtgatagaaa agcctttcat
     1621 cagccaatct acttgttcag gtgcgagagc tttgacatcc ttttctgtac tgggccaagt
     1681 cagtctgccg ttctcaaagc gtttatataa tagccaaaat ccttgaccat cccagtaaag
     1741 gactttaaag cggtctttac gtccaccaca aaagagaaaa acttgacctg agaaagaatt
     1801 caattcaaag tgggttttaa ccagataagc cagtgaatcg attccttgtc tcatacccgt
     1861 tttcccacat acgagataga cctgccctag gctagataga tgaattgtca tagagcaaca
     1921 ccttatccaa aagctgttct atcatttcga gattgagaga ttgaaaaaaa cttacttcaa
     1981 gcttgccaat acgaatttta agcagaatat catttctctt tttagaatca aaacgacgag
     2041 attgtggaat ctctacagga acaataggtt gtggcattaa aatatcctcc aatagtttta
     2101 ctttactaat agtatacagg aggagctcca tgaatgatag ataccttgtt atgacgcgct
     2161 taccatatta gaaagttggc tctctaatta aattaattct cattatatat atcaaaaaac
     2221 cgcagcttga aaactacggt ctaatttttt gtccaaatgt aaaccacttg gaactattat
     2281 tcaatttatt ctttttagca aatgttcccg ttttaatccc agttacagtt tttaatcctc
     2341 tgtaaccgtt gattttacaa agcttttaat ttttttgcga ttattcccac tcaactgttg
     2401 caggtggttt acttgtaata tcgtagacga tacggttaac atgatccact tcatttacga
     2461 tacgtactga gattttttga agtacttccc atggaatttt ggcaaaatca gcagtcatac
     2521 catcgataga agtgatagca cggattgcaa tcgtgtagtc atacgtacga ccgtcaccca
     2581 taacaccgac tgagcgaacg cctgtgttaa cagtgaagta ttgccaaata tcgcggtcaa
     2641 gtccagcttt agcgatttct tcacgaagaa tagcgtctga ttcacgaacg gtttcaagtt
     2701 tctcttcagt gatttcaccc atgacacgga tagcaagtcc tggtcctggg aatggttggc
     2761 gccatacgat gtggtctggc ataccaagct ctgtaccaag agcacgaact tcatccttgt
     2821 aaagagtatt gagtggttca atcagttcaa actgcatatc ttctggaaga ccacccacgt
     2881 tgtggtgtga cttgatagtt tgagctgtat ccgtaccaga ctcgataaca tctgtatata
     2941 aagtaccttg agcaaggaat ttcacatctt tgagcttgct tgcttcgtca tcgaatacat
     3001 agacaaactc gttaccgatg attttacgtt tttgttcagg atcagaaacg ccagcaagtt
     3061 tgtcaaggaa acgtttagca gcgtctgctt tgacgatatt caaaccaaac ttaccaccga
     3121 gcatgtccat aacttgatca gcttcgcctt tacgaagaag accgtggtct acgaagatac
     3181 agatcaattg atcgccaatc gctttttgga gaagaacccc aacgacagat gagtcaacac
     3241 cacctgatag accaagaagg acacgtttat caccgacggt ttcacgaatt tttttgatct
     3301 gcatatcaat gaaattatcc attgaccagt cagctttagc cttacaaatg ttaagggcaa
     3361 agttacgaag gatatcattt ccgtatacag aatgacgaac ttctgggtgg aattggatac
     3421 cgtaaatgtg tttatctggg ttttcgatgg ctgcgtatgg gcagtcagct gatgtacctg
     3481 tacgaacaaa gtcagcagga atctcagtaa ccgcatcacc atggctcatc aaaacagtct
     3541 gttcatcagg tgttgattca aaaagcgctg atggtgtgtg agttagggtt gattgaccgt
     3601 attcacgatt tccagcatca cctgcaggaa caacttttcc tccaagttta tgggtcaata
     3661 actgcatacc ataacagatt cccaaaattg gaattcctag ttcgaagatt tctgggtcaa
     3721 tgtcaaatga accatcttca tatacagaat ttggaccacc tgatagaata attcctacag
     3781 gattgacttc acgaacttca gcagctgaaa ttttatggct ttttagttct gaaaaaacac
     3841 caatctcacg gatacggcgt gaaatcagct ggttgtactg gctaccatag tccaatacga
     3901 tgattttttc tacatcttgc aaatcagttg aaatgttgct catctttttc ctttctcaaa
     3961 agaaatatct tttctcaata ttctatcaca aataagggcg aattaccaac taaacaaggc
     4021 gaagtttgac tttttctagt ttattggggt acaatagaga aaagatagaa atgaagtgaa
     4081 aaatatgcta cctgcttata tgaaaatcca tgatcagata aaaaaggata ttgacgagca
     4141 ccgttgggct attggtgaga ggcttcccag tgaaagagat ttagctgagc agtttgcggt
     4201 cagtcgcatg accctccgcc aagccgtatc tctattagtc gaagaaggcg tcttagagcg
     4261 ccgtgtagga agcggcacct ttgtttccag tactcgagta caagaaaaga tgcgagggac
     4321 aaccagtttt actgaaattg tcaaatccca aggtaaagtt ccctctagcc agctcatttc
     4381 ctacaaaaaa accattccca atgagcaaga agttgccaag ctaggaattt ttccaacgga
     4441 caatattatc cgaatggagc gggtccgcta tgccgaccaa gttcccctag tttatgaagt
     4501 tgcttctatt cctgaaaaat tcattaagga ctttaaaaaa gaagaaatca ccagtcattt
     4561 cttccaaacc ttgcaaaaat atggctatcg tatcggcaaa tctcaacaga ccatctatgc
     4621 tcgccttgct aaagaaaaga ttgcccacta tttggaagtt gaaaaaggac atgctattct
     4681 tggattgaca caggtttcct acctagaaga tggtactgct tttgaatacg taaaaagtca
     4741 atatgtaggc gaacgctttg aattttatct tgaaaataat tagattacca cgcaaaaact
     4801 ctctgtcaca gacaaagagt cttttttatt tttctaagaa taaatctttc aaagaaatca
     4861 aagttacagc aaacaatatc attgccatac caataaaatc aatgggataa aattgttcat
     4921 tcattattaa gaaggcaaag aaaaccgccg atattggctc aattgaagcc aacaagcttg
     4981 acttgaccgg tcctatcaga ctggctcctt taaggaaagc tgtataggca aagacagtcc
     5041 cgataaggat aatgcctgca aacgcaagga gaaaatcaag actagtcggg atatcggcct
     5101 gtagaacccc tgtaaaagga agggcgacca aacctgctat gaccattccc acaccaatga
     5161 ccaagctgct cccccacttt ttaatcaagg ctatgggtaa aatgatatac agagcataag
     5221 tcaaggcaga aaagagaccc cagaacaggc cagcaggtgt catggataac tggtccaact
     5281 gcccatgtgt tgcgatcagg aaggttcctc cgatggcgaa tatgatggaa actatctctc
     5341 ccagtgtcgg tgccacccta tccttgatac agctataaat taaaattccg acaggacaaa
     5401 catactgaag caccgtcgct gttcccgcat tggtctcctg aatagcagac agataggcga
     5461 attggttgag aaaaagacca atcagagcaa aaataagaag agacagcaaa ctctttctat
     5521 cctttaaaaa gaccagtatt ttatcctttg cagtagcata agccaagagc atgagaattc
     5581 caccagcgat taaaagacgc aagttggtta agaccagagc cgaaattccg tgtgccatca
     5641 ggtattggcc actcgttcct gacaaccccc aagcaatacc agccacaact gttagtaaag
     5701 tcccttttaa actatttgac atgtctctcc ttactcttct ttgcgaatca agttcatcac
     5761 ttgattgcgg tctaatatcc attgagctct ctcgtccttt tcataggtac gattcgatag
     5821 gaaaatagtg gcttcttgct tctgacgatt ccacatgata aaggtacctg tatagcccgt
     5881 atggtctagc caatctcctt ccaaattcca tgctaaagaa cgttccttgt catccaaagg
     5941 agaaaaattt tgatttaagt ctcttgcaaa atcatctgct aaatagtgtt ctaaaaagat
     6001 ttgtaaatcc tttatagtcg aaaataaacc agcactccca gcatgtctac ccaggagacg
     6061 agccttggga tcatgcacta tgcctgcctc tacacctcta actgttggaa cagcaagctc
     6121 aactggccca aacttggttt ccgtcattcc ccaaggtttc cagacttgat cctttaaaat
     6181 cacatccaaa tcttgattaa aaattctttc caaaataaag cccaacagca aaaaatggac
     6241 atccgaataa agaaaggctg gctgacttcg tctgttgaga tgaaacatcg cttcctttaa
     6301 ttcaggggct gttaaaagat cacgattagg aataaaagga tcaaggtctg ttgcatgagt
     6361 caagagctgg cgaatagtga tgtctggata atcactctca ggtaaaaaat ctattaccag
     6421 tctatcaata tctaattgac ctatttccca caagaaggta caaactgtgc caaccccaac
     6481 aaccttgctg acactagcta ggtcataaac tagtcctgcc tcagtctgca agccatgctc
     6541 tgggtcactc tggcctaaat agaactctgt ccattgattg tccttaaaat acgcaaaaga
     6601 ggctccggga taaattcctg cctcgatttg ttcttctatt tttttaataa tcttggtcca
     6661 cttcatactt cttcaaacca caattcaaca ttatttctat ctttaccaag gaagaatttt
     6721 tcagacttag gaataaaata ttcagtagac tcaaacttct ggcgaagact tgctatgtct
     6781 aattcattga ccaagaactt gagcatagat aagtcccagg taaccgtatt gtccacagtc
     6841 aaatcctgcc cctgagctgg aataaaatca agggatgccc caatttcaga tgattccaag
     6901 aaactttcga tatcagttgg gagatgtaat tccatagaaa tctcaaattt acttaaagaa
     6961 attgatgcca aatctgtttg aaattcaggc ttttctccta cttctactag acttgctctg
     7021 tcatcttccg catgaatcaa aatcaaatca tcttctggtg agaaaatttc aaaagcgtag
     7081 ccattttgac ctttatataa tcgatgaatc gaatctgttt tagataagat tccttcaatt
     7141 tctaagggat tttccacctt gacaatcaat ctagctagtt tttttcttcc ctctacctta
     7201 cgagtacgca tactgggagc ttcttctaaa accagctttt caagacccgt ttggtcacct
     7261 agtgacagaa aggccgattc ttctaacaag gccttcattc caagggtttc aatataaaat
     7321 gtttcattta attttctatt attagctttt aaagtaggaa taatccgtac aatctgattt
     7381 acattcataa attcctccgt cactttttat tttaaaggat tttagaattt tttacaagat
     7441 attatgctca aatttaacat tatttagcta tttagtaaag atagtctttc aaggcttttg
     7501 acaaagcctg ataaccagca atactgaggt gcagtccatc agttgtatat tcttttttga
     7561 gttggcctgc ttggtctgtc aaacaatcaa atactggcac aaattccacc tgcatatagg
     7621 cagatgcaag ctcttgatag gcttgattcc aattctgaat tttttcattc gagcggatat
     7681 agactgcctg ctggtacttc tctccctcat tgacaggcaa aatggaaagc aatttaatct
     7741 ctgtcaatgg ataatcgcga gcaacggatt gaatgatagc ttcgagacta ttgagagcct
     7801 cattcacagg aacatccttt ccgatatcat ttgtcccaat cagaagaaaa attttatcta
     7861 ctgctccacc atatagatga gcatcaaggt tctctagtaa cagtcctgtc tgatagccac
     7921 gaattcctcg attgacaatc gtctttgaag tcccaaatag ctcctgtaga ggataatact
     7981 cgacaatgga atccccaata aaaagaatgt ttggttctac aacagaaatg tgatttaggt
     8041 gacgatattt ggtttgaatt ttttcttgtt cctttaggag ccaattttct aataactgta
     8101 ctgccacttc attctccttt ttctaaccag ttttccaaga cttgataaac tcctccttgg
     8161 ctgttggctg gtgctagagc agtcgccaca gctttggctt tctcatcagc attttccatc
     8221 gcataggcaa ttccagccat ttcaagcatt tcaacatcat tttcactatc accaaaagcc
     8281 atgatttctt gggatttcaa gtcccagcgc ttgagtaatt cctccaagcc ccatgcttta
     8341 tgaatcccag cttggaggat atcaatgcaa ccatagccac tggatacagc tcggacacgg
     8401 ccatcaaaga gagcattgat ttcttccaaa accgaactca aacgttcctc accaacaacc
     8461 atgctcatct tgagcacacc accaaagagg tcagatgtta attcatccac aaattgcatc
     8521 cgttggtaga atttttcaat catttctgga gtcataaaac tttcaagatc tgtaaaaatc
     8581 gtaccttcct tgacaaaatc acccttcatc cccgttacaa caaactggtc ctgacacgct
     8641 cgacccttga aatgagccaa agccttgttg acaatggcgt catcccatgt ctgagcctga
     8701 atcaattcat tgttttcaaa aatacgagcg ccattagcaa caaccagaac cactcgatcc
     8761 accaagggac tcagtagttg tctcatgcgg tgaatttcat tgcccgtcgc aatgacaaaa
     8821 cgaatgcccc tttgatccaa ctgatctaaa atcttttcca atcgtgggag atcaagctga
     8881 cctctagcat ccagcaaggt cccatccata tctgtcgcaa ttaccttaat catcatttac
     8941 tttcctctgg attcaagcta ataccacttc aatcccacat gttcattcat ttctttatag
     9001 gctgtatcaa ttcgttcctt agtcgcttca tctaactggt cacgatgact gccatcctct
     9061 ataaaatctt ccaaaatata agtttggtaa tgatacaagg gatatccttc tggtgaaaag
     9121 gttccctttg gtgttcgatt gacccaagta ggatgagctt tagctgttcc gatagttgtt
     9181 tttccatcct tcttcttgat ggtgacatcc atgagaacac cacgttcagt ccacttagca
     9241 ttctcttcat ctcccataga ttcaatccgt tgattggaaa tgaagttccc catttaatag
     9301 ataatgagtt tcttatctcc atctttttca accgtttcag atggttcaac aacgtgagga
     9361 tgccctccaa agataatatc cgctccccaa tcgatcatct tgtgataaag agctttttgt
     9421 tcttcagtta gttccaatcg atactcaaca cccatctgaa gcatgataat ggtgatatct
     9481 gcttccttct ctgcccgttc aatttcaacc ttcatcttat cttcgtttaa atctgaaaga
     9541 taacgattat agtcttcctg agaaatatac tgctcaattc cattgaaacc ataggaatag
     9601 gctaacaatg caaccttgat accattcact tccttaatga ccagcagagc ctgatcacgt
     9661 ggttcgtgcg tataaactcc gattggagtg attccagctt tctcaataat atcggccgtt
     9721 gaaataactc cctcaatttg cgaatccaaa atatgattat gagctaaatc cagcacatga
     9781 taacctgcct ccttaatagc atccataact tcagcaggag cattaaagag aagataaccc
     9841 gctaaataat gatccttatt aatggttcct tcaaaatcac caatagctaa atctgctgct
     9901 tagtcccctt gcttgagcca aggagtcaca tactcaaaat tttcatgaaa gtcataggta
     9961 ccgtcttctt ttttagctga aaagaaaagt ccatcgtggt agagcaaatc cccgtgggcc
    10021 ataattctgg cagttttttc ctcttcctac tcctgagact tttgcttagt cccctcttga
    10081 gaaatagtat ctcgtttttg actagtcaag ggattccttg gaagctttca aacaacaaga
    10141 ccaagcccat tgataaatca actgctagca agagtatcgc cacaaatccc ttattgctcc
    10201 acttgcgata actcctaaaa agtttaccaa gcccttcata aaacgaaaag ctaaaccacc
    10261 ctgatttcga ttttgtcttc tttgtatctt cattctccct actttcttat gcaagccttt
    10321 tctttttatt atatcacaga taagtatttc tttcacaatt gaattgaact tcccatctat
    10381 tttctataaa tcctaaatgc cataatgctt tcaattcttg tcattttgtg atatcatgta
    10441 gaggaaatga actaatccac agtggcttat tccaagtata ccacttgggc tttggcagta
    10501 gctaactgca ctaaacataa tataaggaga gaaaatgtct gcaatagaac gtattacaaa
    10561 agctgctcac ttaattgata tgaacgatat tatccgtgaa gggaatccta ctctacgcgc
    10621 gattgctgag gaagtcactt tccccctatc tgaccaggaa atcatcctag gcgaaaagat
    10681 gatgcaattc cttaaacatt cccaagatcc tgtcatggct gaaaaaatgg gactccgcgg
    10741 tggtgttgga ctggctgctc cccagttaga tatctcaaaa cgcattatcg ctgttttggt
    10801 acctaatatt gttgaagaag gcgaaactcc acaggaagcc tacgatttgg aagccattat
    10861 gtacaatcca aaaatcgtct ctcactctgt tcaagatgct gctcttggcg aaggagaagg
    10921 ttgcctgtct gttgaccgta acgtgcctgg ctatgttgtt cgccatgccc gcgttactgt
    10981 tgactacttt gacaaagatg gagaaaaaca ccgtatcaaa ctcaaaggct acaactccat
    11041 tgttgttcag catgaaattg accacattaa cggtatcatg ttttacgatc gcatcaatga
    11101 aaaagaccca tttgcagtta aagatggttt actgattctt gaataaagaa aatcccgttg
    11161 caagacgggg ttttgtgtta taatagaggc atgaaaacaa atgatattgt ctatggtgtc
    11221 cacgccgtta ccgaagctct ccttgcaaat acaggaaaca aactctacct ccaagaagat
    11281 ctccgaggta agaatgttga gaaagtcaag gaactagcta cagaaaagaa ggtgtccatt
    11341 tcttggacat caaaaaaatc tctctctgag atgactgaag gtgctgttca tcaaggtttt
    11401 gttctacgag tgtctgaatt tgcctatagc gagctagatt acatccttgc aaaaacacgc
    11461 caagaagaaa atccacttct attgattcta gatggtctaa ccgatcccca taatctgggt
    11521 tctatcttgc gaacagccga tgcgaccaat gtttcaggtg tcatcattcc caagcaccgt
    11581 gctgtcggag taactcctgt cgttgccaaa acagccacag gtgctattga acacgttcca
    11641 attgcccgag tgacaaacct cagtcaaacc ttagataaac tcaaggatga aggcttctgg
    11701 acctttggaa cggatatgaa cggtactcct tgccacaagt ggaatacaaa agggaaaatc
    11761 gccctcatca ttggaaatga aggaaaaggt atctctagca acatcaaaaa acaagtcgat
    11821 gagatgatta ccattccgat gaatggacat gttcaaagcc ttaatgccag tgttgctgcg
    11881 gccattctca tgtacgaagt tttccgaaat agactataaa aaagtttcca gtcatctgat
    11941 tggaaacttt tttatgatta actatgttct gtgataaact tgtaggcttc ttgaccagcg
    12001 atagctccat ctccaactgc tgttgttact tggcgaaggt ctttcaagcg aacatctcca
    12061 actgcaaaga taccgtcgac tgcagtcttc atgtggctat ctgtcacaat ccagcctgcc
    12121 tgatcttgga tattcaattc tttaacaaaa tcgctaagag ggtccaaacc aacatagata
    12181 aagacaccac cgaaggcttg ttctgtcact tgacctgttt tcacattttc aaatacgact
    12241 gattctactc ggttttcacc cttgatttcc cttactacag aatcccagat aaagctgatt
    12301 ttttcattcg caaaggcgcg atcttgtaaa accttttggg cacgaagttg gtcacgacgg
    12361 tgaacaatgg taacagtctt agcaaaacga gtcaagaaga gggcttcttc aacagctgaa
    12421 tctccaccac caactaccaa taaatcttgg tcacggaaga aagcaccatc acacacagca
    12481 cagtaagaaa caccacgact gttcagttct tcttctccag gcactcccaa aggacggtgt
    12541 ttagaaccag ttgctacgat aactgtacgt gtttcatatg tttggtcatc agtcatcact
    12601 ttcttaaaat caccatggtc ttcgacattt tcaacataac cataaatgtg ctcaacacca
    12661 agattttcaa gtggttcaaa catcttttca gccaattcag gtccactaat attagcgtat
    12721 cctgggtaat tttcgatatc agatgtatta ttcatctgac cacctggcag accaccttca
    12781 atcaaagcta cttttagatt gcttcgagca gcatacaagg ccgcagtcat ccctgcaggt
    12841 ccagcaccga taataatagt atcgtacata tagattcctt ctttcttgtt gtaactatct
    12901 ttattctaac tctttcttgt caatcacgca aggattatgc cttgaaaaca agaattaaac
    12961 tcataaccgc aaaggataat actgggacaa ccaagactcc aatcataatc atatcataga
    13021 gatgggcttg gcgagccttg gctgtcttat caactcccga catggctctc agaccaatcc
    13081 aaatccctaa aaaaatcaga acaaggatgg tggtcaatat caaactctcg aaatataaag
    13141 aaaatagttg cagtagcatg atttctctca tttctatctt ttttaaagag taaactcagc
    13201 tagtccaact aactgagttt tcctttatct attatatcaa atataagtcc gtttgtaact
    13261 agcgaagaat tcttttgtcc gctcttcttt aggggtgtgg ataatctcat ccggagttcc
    13321 agactcgatg attttcccct tatctaagaa gagaatctta tccgcaactt gggctacaaa
    13381 ggacatgtca tgactgacca aaatcatggt ctgacctgac ttagcagcat ctgcaataga
    13441 cttttctact tcaccgacca attctgggtc aagggctgaa gttggttcgt ctaagagcaa
    13501 aacatctggt ttcatagcaa gcgcacgcgc tagggcaacc cgttgcttct gtccacctga
    13561 taaatggcga ggataatggt tttcacggtc cgaaagccca accttagcca actcttcctt
    13621 ggcaatctta gtcgcttctt ggtcagataa tttcttgaca acaaccaagc cttctttcac
    13681 attatcaagt gctgttcggc gttcaaacaa attaaactgt tggaaaacca tagacaactt
    13741 acggcgtagg gcaaggattt cttcttgagt gattttagaa aaatcaactg aaaaaccatc
    13801 aatctgaata gagccactgt caggtgtttc aagataattg agactgcgaa gaaaggttga
    13861 ttttccagct cctgaagaac caatcaaggc tacaacttcc cctttttgaa tatccaagtc
    13921 cagatgatcc aagacagtct gtcctgaaaa ggatttgctt aaattcgaaa tcttaatcat
    13981 taacgaaggt ctcctttcac atctgtttgc actgtatcag gtgcagaaat agccattttt
    14041 ctctcgatga aacgaccgag gctttcaatt ccgatattga ctacccaata aacaagggca
    14101 acggagatga agcgttcaaa atagcgataa tcagctccac ctagaatctg agcttgggca
    14161 aagacttcca caacacccgc actaaaagct agagatgttc ccttggtcaa accgatgagg
    14221 gaattaatca aggttggagt agctaccacc gctgcattag gaataatcac tcgtcgataa
    14281 acttgcgctc gggtcatacc cagactgcgt gccgcctcaa tctcaccagg attaactgag
    14341 agaatggctg cacgaatggt ttcactagca taagctgcct cattaaaggc aaaagcgaca
    14401 atcgcaaaag ctgcagctgg aatcgcattg atattgagac cagttcccca ttgctgattg
    14461 agggctttca aagccaaagg gattccgtag taggtcaaca tgagttgcac caaaatcggt
    14521 gtccctttta agaaactaac aaagaaggcc tgcaagggat ataaaatctt gacacgattg
    14581 atcttcacaa tggcaaaaag aagcgccaaa accaagccaa aaagggcacc accaattgtc
    14641 aacataattg ttgttggaag ttgttggaca attctaggga ttccatcaaa gaccgaacgt
    14701 aggctaaaca gcttgccatc cggaatcaat tgcatcaagt tttggtacca atctgatgct
    14761 aaaatcgttg taacattcat aaaaacatcc tcttctgata atgattcatt ctaccatgct
    14821 tctgccgtca atgaaattat ttgctacggt cagatacaga ctttgtctac ctactagttt
    14881 atcatacttt gaaacaggga agttatatat tttatctatc aaagagatag ataaaaacta
    14941 tttcagtccc agttcttcat aagattttcg ataaccgatt tgcttaacag ttccattttc
    15001 tactaaaatg ggccgtttta acaacatacc gtcacttgct agcaactcag ccgcttcttg
    15061 gtttgacaaa cttcctacct tatcttttag ccctaattca cggtatttga taccactggt
    15121 gttgaaaaat tgcttcaatt caaatcctga ggtttctagc caattcaaaa tgacttcttg
    15181 gctaggtgtt tcttccacga tatggacggc tttatagtcc acacctaatt gatttaattc
    15241 ttgttttgct tttttacaag ttgaacattt ggggtattcg ataaattcta acatgtgctt
    15301 ctccttctat ttgtttatat ctttaaaatc tacgccttca tgctccaaga gccaagcttt
    15361 cttttccact cctgcagcat agcctgtcag acgcttgcct gctcccaaca cacgatgaca
    15421 aggtactagg atagaccaag gattgcgtcc cactgctcca ccaattgctt gagcagaagc
    15481 cacttgcagg tcttgagcaa tttgtccata ggtcactgtc tgaccataag gaatgccctg
    15541 taaatagtcc caaactctct tttcaaaatt cgttccgatt ggcgccaaga gcaagttgga
    15601 taaatcctga ggcttgcctt taaagtaatc atctaagcaa gcaataactg ggtctaaaat
    15661 aggatgacta acaacttctt ctatcgtttc atctcctagt cccctctcaa aatgcttctg
    15721 ctcctgaacc caaattccat acaaataatg gtcatcagct acaagtgata gaataccaat
    15781 tggtgaggag tagaggattt ttacatactt cttttgcatt atttctttaa tttttgatag
    15841 gccaagcgtt cgccatctac tggcacctta ccaacttgtt taaaaccaag tttttcaaaa
    15901 atatgttgca taaccttgtt ttcagcatgc gtatctgagc gaaaatcaag ataatcaaaa
    15961 ccttcaatca agccctctaa gaaggtttga gcaactcctt ttccctgcac atctgctgcc
    16021 acagcaatac ggtgaaagac tagatactct gactctccag cttgccagtt tccctcataa
    16081 atagcttcat aggcctcctc tggactcttg gtcacagcag cataggctag cagttctccc
    16141 tcttccaagg ctacgtaggc ttgccctgag atgatatcat cgataataac atctgcattt
    16201 ggatagccat tttgccactg gtcactacca gcatctgcta aacatttttt ggcatcctct
    16261 atcacctgca tgatggcacc tacttcattt ggaaaagcta aacgaatttc catctttttt
    16321 cctcatttct gactagtttc tcccatcata gcataattta agtctttcta caagcagtta
    16381 aaacttgact ttgtattttt gttattttat aatctagtta tcaaaactat ataaaaagga
    16441 gttgaaaatg aaaactacct tttcctaccc aaaatgggca gaaattccaa acattaacct
    16501 ctatctggac caggttttgc tctatgtcaa tcaggtctgc gcccctatct ctcctaataa
    16561 agacaaaggc ctaacagcat ctatggtcaa caattatgtg aaaaatggtt acctgacaaa
    16621 gcctgacaaa aaaaaatacc aacgccaaca gattgcccgt ttgattgcta tcacaaccct
    16681 caagtctgta ttttctattc aagaaatagc ccagacactt aatactctac aaactcaagc
    16741 aagttcagac caactctacg atgcttttgt ggactacatg aaccaaggaa ttgacccagc
    16801 taaccctatt atccaaacca gctgccaaac cgttaaactc tatcatcaaa ctctagactt
    16861 aatcgatcat actcaagagg aggtaatcca atgaacacta gtcttaaact cagcaaacaa
    16921 ctcagttttg gagaggagat tgctaatagc gtgacccatg ctgtgggtgc agtcatcatg
    16981 cttatcttgc tgcctatttc atccatctat agttatgaag cacacggatt tttatcctct
    17041 atcggcgttt ccattttcgt catcagtctc tttctcatgt tcctatcatc caccatttat
    17101 cactctatgg cctatggttc gacccacaaa tatgttttgc gaatcattga ccattctatg
    17161 atttacgttg ccattgccgg ctcatacacg cccgttgtct tgaccttgat gaataactgg
    17221 tttggctatc tgattattgt catccaatgg ggaacgacca tctttggtat tctctataaa
    17281 atctttgcta aaaaggtcaa tgagaaattt agccttgctc tttacctgat tatgggctgg
    17341 ttggttctgg ctatcattcc tgccattatc agtcaaacga cacccgtttt ctggagtctc
    17401 atggtaactg gcggactctg ttatacagtt ggagctggat tttatgccaa gaaaaaacct
    17461 tatttccaca tgatttggca tctctttatc ctagctgcgt ccgcactcca atacatcgct
    17521 attgtttatt acatgtaaaa aagttgagaa gttcaatctc aacttttttc tttacacata
    17581 ttgataaagt actggtgcaa gcgcacatca tcagtcaatt ctggatgaaa agaacttacc
    17641 aacatatttt tttcttgggc tgcaacaatt tgattgttca ctgttgctag aatttctaca
    17701 ccctcaccaa cactactgat aatcggacca cggataaagg tcattggaat cttgccaact
    17761 cccttacatt ctgcttccgt gtagaaactt cctaattggc gcccataagc attacgctcg
    17821 accaccatat ccatagttcc tagatgactc tctttctgag aagtgatttc cttagccagc
    17881 aaaattaagc ccgcacaggt cccaaacact ggtaagccag atagaatggc ttctcggatg
    17941 ggaagtagca tgttctggtc acgtaagagc ttgcccatgg ttgtagactc accaccaggc
    18001 aaaatcaaac ccgacaagtc actctgatct tgctgaaaat catctagatt tctgagttct
    18061 acactctcga cacctaattg atctagcact tttgcatgtt ctgcaaaggc cccttgcaag
    18121 gccaatattc cgattttcat ctattttcct cgttcagcca tgagaatttg gatttcattt
    18181 tcattaatac caaccatggc ttctcctaaa tcttcagaga tttgagctag gatttgagga
    18241 ttacggaagt tagtcacagc cttaacaatg gcactcgctc gtttaacagg atctcctgac
    18301 ttgaaaatac ctgaaccgac aaagaccccc tctgccccta attgcatcat taacgcagca
    18361 tctgctggcg ttgcaacacc tccagcagcg aaatttacaa ctggcaattt tccatgttca
    18421 tgaacatatt ggaccaattc tacagggact tgcaaatcct tggcagcaac ataaagctcg
    18481 tcctcacgta agttttgaat gcggcgaatt tcctgattca tcatacgcat atgacgaaca
    18541 gcttggacga tatcccctgt ccctggttct cctttggtac gaatcatgga agcaccttca
    18601 gcgatacgac gcaaggcttc acccaaatcc ttagcaccac agacaaaagg aacttggaat
    18661 tctttcttgt ccacatggaa acggtcatca gctggagata gaacttcact ctcgtcgata
    18721 taatcaattt caatagcctc taaaatctga gcttcaacaa aatgcccgat tctgacctta
    18781 gccattactg gaatactaac cgcttcttgg atttccttaa tcatctttgg gtcgctcatg
    18841 cgggaaactc ctccagctgc acgaatatca gccggaattc gttccaaggc catcacagct
    18901 gccgcaccag cagcttctgc gatacgagcc tgttcaggat tctgcacatc cataataaca
    18961 ccacccttga gcatctgtgc caagttttta tttagttcat aacgattttc agtcatttgt
    19021 tttatcctca tcatttgtat tggtagctac cttttcattt taagtcagaa cagaatgaag
    19081 cacaagataa aaaaactata attgtatggg gacagattgg ctaaaaacta tctgacctta
    19141 acttaaggcc agatagctca ttcatttcta tttttcagcc gtaagggcag ccattgtgat
    19201 gtagttgtat ggtttgttga agtgtggcaa gaagaagagg tctgtcaatg ccaatttatc
    19261 aattgtcaca tgctcttgga tagcaagtga gaacatgtgg attcccatgc taattgcaat
    19321 atcatgtgaa accatttggg caccaagaat ttcacggcta tctttgtcaa agacaatctt
    19381 aattgctact tcatggttgt catgtttcat gaattctggt ttttgaagat cgttaaagcc
    19441 tgtttcagtt gcgttgtaac cagcagcttt cgctttttca agagtcaaac cagttgaaac
    19501 catgtgaaga ccgtagattg agataccatt tgaaccttga acaccgattc cttccaattc
    19561 atgtccacaa gcattgtagg caccaacgat accagtgcga acagcatttg aagcaagagc
    19621 gatatagctt gtatctttac gagcattgtc ataaacagtc gcacagtcac caacagcgta
    19681 aacgcctggg atagatgttt cttgtttctt gtctacaagg aaggcaccgt tgcggaagag
    19741 ttcgatctta ccatctgcaa gggctgtgtt tggacggaaa ccaactgcaa ggataaccat
    19801 atccacgtca aagctttctt tgtcagtaat caagcgttca actttaccgt caccttcgat
    19861 tgctttaaca gtttgaccta gagccaagcg gatgttgtga tcttccaagt tcttcgccat
    19921 catttgtgtg aagtctttgt catagtaacc gttcaagaca gtatcaacga tatcaacaag
    19981 gacaacttct tttccaagac gttcaaaggc ttcagcaagt tcaacaccga tgtaaccacc
    20041 accaacaacg gcgatacggt cgaggtgttg gctcttgtca gaaagtttat tgataacttc
    20101 ttcagcattt tggtacaatt tcacgaattg tacgttttca agagttgctt taaattcgcg
    20161 gtttccttta acaatttcaa caccttcgat tggtggcaag attggtgtag aacctgtagc
    20221 gaaaatcaat ttttcgtatg attctttgtg ctcttttcct tcaacttccg ctgtaactac
    20281 tttgttatca tagtcgattg aaagaacagg tgagttcatg taaactttag cacctttagc
    20341 ttccaatttt tctttatcag aatagaacaa gccttcagca ccgtcaattt gttcaccaat
    20401 ccaaagagcc attccacatc ctaggaaaga gatgttagag ttttggtcaa atacaacaat
    20461 ttcgttctca tttccaaaat tatccaacat ggtattgata catgctgtac cagcgtggtt
    20521 agcaccgact acaacgattt tactcataaa aatttcctgc cttcattcta aatttacctt
    20581 actagtatac catttaccgt tagcgtttac aaggggggta ctattatttc agcatttttt
    20641 atcaaaaata attccccatt tatcctgttt tcaagcctca tgaattttat gagcatattt
    20701 cttgactttt tccaaatttt gtttgtgaaa attctaactt tatggtatta taataacatg
    20761 aaaatgaaat gtaagggctt caatttagat gattatttga tattacaact tattttctat
    20821 aagagaaagg agttggtagc ttgactcttc gttcacattc tgaacggcta atggggacta
    20881 ctatcactat ttcattagtg gatgagcaag ccgatatctt tctccaaaaa tcctttgact
    20941 tgctcaaaga acttgaatac cgcttcaacg ccaatagtca agaatctgag ttgatggaaa
    21001 tcaattatca agcaggaata gcccccgtca cggttcatcc agacctgttt gagctgattt
    21061 cacttgggtt agagcatagc ctagcgctct ctagccacct caatatcagc attggtccct
    21121 taattcaaac ctggcgtatc ggtttttcag atgccaaggt cgcccagcct caagaaattg
    21181 aatcggtgct gcctctaatc aatcctcatg gtatcgagtt agattcttct acttctactg
    21241 tgtttttaaa acagaaagga atgaagattg atctaggttg tttagccaaa ggatacagtg
    21301 cggataaggt tgcccaattt cttaggaaag agggggtgac ttctgccttg atcaatctgg
    21361 gagggaatat cctgaccatt ggaaaaaatc aggcaagagg ggataaccca tggcaaatcg
    21421 ggattcaaga cccagccaat cctaggggaa atcatttaat gaccatccct gttgtcaata
    21481 aatctgtcgt gacttcaggc atttatgaac gtcacctgac cgtcgatgga caagattacc
    21541 atcacatttt tgacagtcaa acaggatatc ctgttgaaac ggaactagcg agtctaacaa
    21601 tcatctctga taaatcagtc gatggcgaaa tctggacgac tcgactcttt ggagaaagac
    21661 ctgcttctat cctctggcaa gtcgaaagtt tggagggcat cgaagtcatc ctcatcgata
    21721 aagaaggcca cctaagctgt tcttcaggaa ttcctactct atagagacaa ctgtttcaat
    21781 ggaattttaa aattgtatta tgtaaaaaga gaaaggaaat ctcatgttaa aacttattgc
    21841 tattgttgga acaaattcaa aacgttctac aaaccgtcaa ttgcttcaat acatgcaaaa
    21901 acactttgct gacaaagctg aaattgagct tgttgaaatc aaggccattc ctgtcttcaa
    21961 caaaccagct gacaagcaag tacctgctga aatattggaa attgctgcta aaatcgaaga
    22021 ggcagatggc gttattatcg gtactcctga gtatgatcac tctattccag ctgttttgat
    22081 gagcgctctt gcttggttgt cttatggtat ttacccactt ttgaacaaac caatcatgat
    22141 tacaggtgct tcttacggta cgcttggttc atctcgtgcc caattgcaac ttcgtcaaat
    22201 cttgaatgct cctgaaatca aggcaaatgt tcttccagat gaattcttgc tctcacactc
    22261 tcttcaagca tttaacccaa gtggcgactt ggttgacctt gatgttatca agaaattgga
    22321 tgccatcttt gatgacttcc gtatctttgt aaaaatcaca gaaaaattac gtaatgcaca
    22381 agaattactt cgcaaagatg ctgaagactt tgactgggaa aatttgtaag ataggagacc
    22441 aaaaagaatg aaatttgttg gacttgttgg atcaaactac gatcaatcat ataaccgtaa
    22501 gctcttggaa tttatccgtc gccacttcaa actcaaattt gaattagaag ttcttgaaat
    22561 tgacgaagtt ccaatgttta accaagatga aaaatgggat gaaagtttcc aattacgtta
    22621 tttatataac aaaattactc gtgcagatgg tgttatcatt gctactcctg agcataacca
    22681 cactatctca gcttcactca aatctgtact tgaatggctt tcatacgaag ttcatccatt
    22741 tgaaaacaag cctgttatga ttgtgggtgc atcatactat gaccaaggaa catcacgtgc
    22801 tcaagttcac cttcgtaaaa tccttgatgc tccaggtgtt aatgcctaca cgcttccagg
    22861 taacgaattc cttcttggta aagctaagga agcttttgat aacaacggaa atatcaccaa
    22921 cgaaggcact gttaaattcc ttgaaacttg cttagataac tttgttaaat acgtaggagt
    22981 cgtttcgaaa ttgaaaaaac caaaaccaat cgaatcagaa gacttggatt gtggaaaacc
    23041 aatcgctaca accatcacag aagttgaccc tgacgatcca gaatgggtag aaaaagttgc
    23101 agcaatcact ggtgctgttt ctggtgatac ctatgtcaaa ttggaccacg gtatcctaac
    23161 agttaaccaa attgatatgt tcttgaaagc tatgccattt gaattgacat acgctgacga
    23221 caacaaccaa ttcctctact acaacaacgc tcaccaagat ccagacacta tgttcgctaa
    23281 acgtgtacca cctcaatcag gtagccgtat gtcgactgtt catggttctc ttccaccagc
    23341 acgtatgaag aacgtagagt gggttatcgg aacacttcgc aacggaaacc aagaatacgt
    23401 ccgtacgatc gttccaggtt ctcctgcagg tgttatcaac actcacaact accaagcaat
    23461 gtactatcct gatggatcat acgctggtat caatgaaatc gtctttaact tccaaccatg
    23521 gcttgactgg tacctaaaag agactggtca acgtttggtg ggtggtagcg gaccatttgc
    23581 tcctgctgcc ggaggtcatg gagacgccga tgctacttct ggcgcttctg attcagggga
    23641 tgctggaggc cacggtggtg acgcagacgc tacagccggt gcaagttact aatgatagtt
    23701 tttaggaacc attatggttc cttttttagc gaaaaaagac tagcaaacac aaactgctag
    23761 tctctttgtt atcgtttcat ctatctttgt taaaagaaag agtacatatt atgaatttgg
    23821 gtcatcaaga ctacggccat gtaatccttt ttcacgttgt acttggcgta gtttttctgg
    23881 tgtaacatcg tttccttctt cgtccacaat tttgattcct tcaatgtgat gacgaacagc
    23941 gcgacgataa ccttcgatgt actcctcacg tagtttggct tgttccactt tttcttctag
    24001 tgttaagcct tctgtttttt tctttttagc aagctcattg atacgagcaa tttttttcgg
    24061 atccatttct tctcctttgt aataagataa agtccgttta tcaaatttta tttagtgtta
    24121 atttggttaa aacaagcaaa cttagctgct ttcttggtta gttgtgacaa gattgccaaa
    24181 cgattttgac ggacagcctg atcttcagcc attaccatag tattttcaaa gaaagcatca
    24241 atgactgggc taagcgcaaa gagttgtttc aattgctgac ttgcaggtcc tgataaaacg
    24301 agtgtttcta ctgcttctgc caaagctttt tcttggtcat tctcaaatag tgctgaatca
    24361 actgtagcac ccccttctgc cttctcagcc aggttaaagg cacgagaaag tgattcaaca
    24421 gatggtttaa aatcttcttc cttgcttact tctacgagag cacttgctgc ttccaacata
    24481 tctgccacaa caaagtttga acctgcaaga actgcttcct tgatatcttt tggagtagag
    24541 cccatcatct tatcaacacg agccttgata aagtccataa cctctgcttt attttcataa
    24601 gtcaaactgt caaatttcaa tgcataaagg ctatcaatca gctcatccat agcaatgtgc
    24661 caaccaaagg catccaagat acgaaccaca ccttgagttg cacgacgaag ggcataaggg
    24721 tcattagaac ctgatggaat caatcctact gagaagaaac tcaaaatcgt atccaatttg
    24781 tctgcaatgg ctagaactgc gccgaccttg ctctctggaa gttctccttc agctgatgta
    24841 ggcatgtagt gttcacgaat agcagctgcc accgctggag tttcaccagc aagaagggtg
    24901 tatttttcac ccataattcc ttggagttcg tcaaattcac caaccatacc tgtcaataag
    24961 tcaaacttgt aaatggctgc tgcacgagca aggtcaactg tttcatccac tgacaaacca
    25021 gctttttctg ccaaaagtac agtgatttga cccgtacgaa tcatgtgttc acgaagggaa
    25081 ccaatcttct catggaaggt gacattgttt aatttttcaa caagatctga aatcaccaat
    25141 ttttggtctt cacgccagaa gaattctccg tcttccaagc gggctaccaa gactttttca
    25201 tttcctttga tgacattttt caaacgctct gcgtttccgt tacgaacaga aatgaagttt
    25261 ggcaagagtt ttccatcttg atcacgaaca acaaagtaac gctggtgttc cttcatagaa
    25321 gtcaccaaga cttcttctgg aacttcaagg tatttagcat caaaacttcc catgaaggca
    25381 gttgggtatt caaccaaatt caagacttca ttcagcaaat ccgcatcaat ttcgatacgt
    25441 acaccatgtt ttgcctcaat ttccttgatt tggtcaacaa tcatttgctc acgttcacat
    25501 ggatctgcga ttacaaactg cttacgaagg tcttcttcat agctcaatgc tgactgaatt
    25561 ttggtttctt tccccaagaa acgatggcca cggctcacac gacttccctt gatatcaagg
    25621 aaatccaagt caaactcttg ctcatccaag agaacagtta aagtgtggac aggacgaatg
    25681 tattcaaagc tgtttccagc ccagtgcatg ctgacaggga aagtcaatga cttcaagaca
    25741 tctacaacac caggaacaat ggcttcaact gcttgaccaa cttcttcctt agtgacatag
    25801 acatattctt cacccttgat ttcacggaat tcgatatctt caacagtcaa gccttttcca
    25861 cggacaaatc cttgagctgc tttggtgaag tttccatcac tatccaaggc aattttcttt
    25921 gctggtccct tgaaatcttc tgtcaaatca gactgtttgt ctgataaacc agtcacacga
    25981 acagccaaac gacgtggtgt tgagaaggtt tgaatggctt caaaagacag gcgttttccc
    26041 ttgaggaagg ctgccatttt ttcgcctagt tgtttttcac ttggtgtgac aacataggct
    26101 ggtaattctt caagaccgag ttctactaat aagttttttg tcatgttttt tctccgaaaa
    26161 gtatcttttt attttccagt ttgccttatg tgattggcac gcaagcaacc aacttcgttg
    26221 tttcattgct aaaattggag ccaaaagtct ccaattttgc ctagtatcct tagtttcact
    26281 aaggatacct tcatcactgc ggcaactggc tcagggctca ctctgttcgc ccattttcgt
    26341 aatttgtcac tctctttatt ctgcgtcttc tgctaagagt ttagctcgtg tttcttcatc
    26401 caaaagtggg tagcctagtc gtttgcgttc tgcgacaaag gttttggcta cgacacgggc
    26461 caagttacgg atacgagcga tatagcctgc acgctctgtt acagatacgg caccacgcgc
    26521 gtcaagcaga ttaaaggtat gtgaacattt gagaacatag tcataggcag ggtgtaccaa
    26581 gccttcttct aatgcacgac cagcttcttt ttcaaactta tcaaaatttt caagcaacat
    26641 ttcttggtcc gaaatttcaa atgaatattt tgagtgctca tactcaggct ggataaagat
    26701 ttctccgtat tttacaccat cagcccactc gatatcatag acagagtcta cttcttgaat
    26761 gtaagaagcc aagcgctcca aaccataggt aacttccgca gtcacagggc tagttgccaa
    26821 tccaccgact tgttggaaat aagtgaactg agtgatttcc attccgtcaa gccaaacttc
    26881 ccaaccaaga ccagctgaac cagttgatgg gttttcccag ttgtcctcaa caaaacgaat
    26941 atcgtgctcc aaaggattga ttcccaattt ttccaaagac tcaaggtaaa gttcttggat
    27001 atttgatgga gaaggcttca tgaccacctg gaattggtgg tgttggtaga gacggttagg
    27061 gttttcccca taacgaccgt cagcaggacg acgtgatggc tctacataag ctgcattcca
    27121 tggctcaggt ccgatagcac gaaggaaagt gtaaggactc attgtccccg cacctttttc
    27181 attgtcataa gcctgcataa gcatacaacc ttggtcattc caaaattgtt gcaaagtcaa
    27241 aataatttct tgaaatgtta atttcttaga cattgaatac tccttaataa aaaatgattt
    27301 cttgcgacac gagtctgcct cgtcgattat acgaggcaag acgagttagt actgcgtcta
    27361 gctcagcacc ctagtgctga gcgtggggca gtccgactgt aaggaggttt ctgccactga
    27421 cgcagtgaaa tgtcaatttc ttagacattg tttactcctt tttatataaa ttacttgcga
    27481 cacgagtctg cctcgtcgat catacgaggc aagacgagtt agtactgcgt ctagctcagg
    27541 cactctagtg ctgagcgtag ggcagtccga ctgtaaggag gtttctgcca ctgacgcagt
    27601 gaaatgttaa tttcttagac attgaatact ccttaataaa aaataatttc ttgcgacacg
    27661 agtctacctc gtcgattata cgaggcaaga cgagttagta ctgcgtctag ctcagcaccc
    27721 tagtgctgag cgtggggcag tccgactgta aggaggtctc tgccactgat gcagtgaaat
    27781 gtcaatttct tagacattgt ttactccttt agtttttata tttctttttc aattaatttc
    27841 tgctgaacaa accaatctaa caagggaaaa gttttttctg tatttgccca taaatgataa
    27901 gaatctaaga ccgcttgtac actacctaaa ttttctacca acttatctac tgtcaaaaag
    27961 ctgcgccagt attcataaaa aatacttgca tagttcttat cataagtcga agaaccaaaa
    28021 tcattcaatg aatgccaact atacttcttc tgaaaaagtt ctacgagaga ttgattacaa
    28081 attttttccg cttgaaattc ctcttctgtc aagaaatact tgcgactaat atattcaacc
    28141 atcccctctt cgaaccagat ataagagtca taaccattcg tctattattt gtataggctg
    28201 gaacagcgct ttccctaata atctgtgtag cagcattaaa atctgaccaa ataacggctt
    28261 gcggtaaatc acagactgca agttcattct ttaaaaaaga ttgataatct ttcaactttt
    28321 cagtatttct tactacaaaa tcacgaaatg tagctagttg actatcgtct tttacaagat
    28381 aaaggttgtc cacggctctc ctttctttca cacaatagac tgaataagca aaagaaccgc
    28441 gtccgacaat cctaggttga tttaacctag gggcgtcaga aacgcggttc caccctaatt
    28501 tattacttca ttgtttttga aaatactaca gaaagcgcca gctcattatt ttgccctact
    28561 tggctcccac tatcccaagc tcgcttgaag aacgccataa gactttcttt cctgctgact
    28621 attatatcaa aaattagaat aatgtacaat tctttttatg attttctaga aatccacatc
    28681 atcaactcgt ggagcacccg attgaacagc aatcgttttt aaggtttctc tctcctcatg
    28741 actcagttca attccaaagc aatcaagatt agcttgaata cgagaggttg tgacagattt
    28801 tggaagtggt aaaaatcctt ctgccaagct ccaggccaag gctatctgag caatcgattt
    28861 tccgtgattt gctgcgattt cttgcacttg cttgctatca aatagttctc cctgaccaaa
    28921 aggcccccaa gcttccaata aaattccctt ttcacgacag taagctacga cttgatcttg
    28981 atacacacct ggcgccaagc gaacttgatt gaccgcagga acgatagttg cagtttcaag
    29041 caaggcatcc aaatgatggg gaagaaaatt gctaacgccg atagcacgga ttttcccttc
    29101 ttgatagagg tcttccatcg ctctccaaac ttccgcattg cgagttttcc atgcgtcatt
    29161 ttctctgagc ggttttgggt tcggccaatg aatcaaatac aaatccaaat aatccaagcc
    29221 cagtttttct atagattttt ccaaagcttg acgagtttgc tcataggttt gctgactatt
    29281 ccaaagcttg gtagttacga acatttcttc acgtggaact ccgctatctt tgattgcttg
    29341 accaacactt tcttcattct gataaatcgc cgccgtatca atatgacgat aaccagcctt
    29401 caaggcttct aacactgcat gataggcttc ttctccatcc ttagccttaa aagttccaaa
    29461 tcccaatact ggaatttcta ctccattatt taattgatag gtattcatat gcttctcctt
    29521 ctattttatt catcaattat agcataagac attttttaag tagtactatc ttctgtaaaa
    29581 aataagtctg tcaaatatct aacagaccta ttttttcttg cttattcatc acgactatag
    29641 tacagctcat gagcttttag gcgagtattg agcatctctg gtatggtcac aaaggtataa
    29701 ccttgatttt tcaaatactc aatgaccctt ggcaaggcat tgactgtcgg actgtgaata
    29761 tcatgcatca aaacgataga gccgttagct acttgatgct gaatttctgt caaaatagat
    29821 gcttcatttt tactcttcca gtccagacta tccacatccc acatgataaa gctcaaatcc
    29881 aagctattgc gaatatcatc tgtaatagca ccataaggtg gacgcatgag tttagaacta
    29941 gaacccagca ctttagttag cacatcctca gtatcagtaa tctgcttttt agcttcatca
    30001 agagagagtt gcgagagaat cggatggctc caactatggt ttccaacaac atgaccttca
    30061 gattttatcc tcttcaccaa gtcctcattc ccagaaacat ttttcccaag cacaaagaaa
    30121 gtcgctttaa tatcatattt agctagggtc tctaatacct gcggggtcgt tgctggattt
    30181 ggaccatcat caaaggttag agcgacaact ttttgatatt tcttatcaaa gtaagattga
    30241 tacaaggccg catctttttc gagtaagtac gaagattgga taacatcaaa gaaagcagat
    30301 actggcaagg ctatctcttc taaattttca agcacaggac ttggataaag gataatctga
    30361 ctatccttgt aatcaaaatt ccatgcagac aagtcttggt cagagaagct ttttacaatc
    30421 tgctcactct ggtcttgctc tattttttta tcctctatga aggaggtcaa ctcttttatc
    30481 agctgttcct tagccttact agcatctgaa aacagttggt caagtgtaaa aggttgccca
    30541 tcttctgtta agtgaacctt tcctagactg gtcttttcag tctcttcaat cttaaaagca
    30601 actaaatcat agatttgttt ggtcacatta cgattaacga ctccctttaa acctgactct
    30661 tcttgctctg tatagtagaa aacaagattg tccttacttt ctagcttgtc cttaacatct
    30721 tgatttatca gctccctaac agaggaaatc actttctccc cttggagagg gtaataggca
    30781 atcacttcgg cttgcccctg acgaaaatac tccttctgat ttccctcact caattgatca
    30841 tctttctctt ttttgagcaa ttcaatcttt tgctcaaaac ttttttgctg gtagatctta
    30901 aaagcaataa atccgcctaa taggcaaata gaaattgcta aaataccaat caaagccaat
    30961 aatacatgtc tcgttttccc gtgtctgcca cgtcccagtc tacttttatc catatttata
    31021 tcataacaaa tttgagcaat aatttcaatg atacaatttg gcaatccaca attcctctag
    31081 caagccaaag catcttacat caactccaac atccgtatct gtagagatta tgtagttttc
    31141 tatctatgga atttctagta gattgaagct agaatagtag acctatactt ctaaaacatt
    31201 gttagaaatc gatttgattg tcctgatcga tttgtcctgt tctcatttca ttttactata
    31261 aaactatgaa aagctaggtc atctgagaag taattactat cgacaattgc gatagcgtat
    31321 actagtgcga tttgtttgta gctctggtgg gtatcacctt cacgttggcg aattttttct
    31381 aggttttgat taacctgact gcacagataa ggccataggc gattgatgaa aaaattctga
    31441 tgatgatcct gaatttcgat gataacttgg attccattat ctaactccgc caagacgtct
    31501 atactagtat agaaatctta cgctgagtag ggcatggaag gcaagacgtg aatgttactt
    31561 ccctccaaaa tcgtcacatt ttttgctggc aagtccagca tatcgcggat aaattgacaa
    31621 atcattagtt gggctgatac ctggatgtct gagaatcctc cttttcctcc ttctattata
    31681 gcacattttt aaatctaggt ttgctagatt ctatgcttct atctatttat tcggaaaaga
    31741 aagctaatag cttatttgaa attttctcct ctctgttgga tagtcccgtc tatctatgtt
    31801 ataatgaaag aaggatttaa aatcggaaaa ggagtattta tgctaaaatt aggtgtcatc
    31861 ggaactggcg ctatcagcca tcatttcata gaagcagccc ataccagtgg agaataccaa
    31921 ctggtcgcaa tctattctag aaaactagaa actgcagcaa cctttgcttc tcgctatcag
    31981 aatatccaac tattcgatca attagaagtc ttcttcaaat cttcttttga tttagtctat
    32041 atcgccagtc caaactcctt acattttgct caggcaaaag ctgccttgtc tgctggtaaa
    32101 catgttattc tcgaaaagcc agctgtcact caaccacaag aatggtttga tttgattcaa
    32161 acagctgaaa aaaataactg ttttatcttt gaagcagctc gtaattacca cgagaaagcc
    32221 tttactacta tcaaaaactt tttagcagat aagcaagtgt taggagcaga tttcaactat
    32281 gccaaatact cttccaagat gcctgacttg ttggctggac aaacaccaaa tgtcttttca
    32341 gaccgttttg ctggtggagc cctcatggat ttggggattt atcccctcta tgctgctgtt
    32401 cgtctttttg aaaaagctaa tgacgcgact taccatgctc aacagcttga caatagcatt
    32461 gacctaaatg gagatggtat cctcttctac ccagactatc aagttcacat caaggctgga
    32521 aaaaacatca cttccaatct tccttgcgag atttatacaa cagatggaac cttgactctc
    32581 aacacgattg agcatattcg ctcagctatt tttaccgacc accaaggaaa tcaagtccag
    32641 attcctatcc aacaggctcc tcatacgatg actgaggaag tcgctgcatt tgcacacatg
    32701 atccagcaac cagatctgaa tctctaccaa acttggctgt atgatgcagg ctctgttcat
    32761 gagctactat ataccatgcg ccagactgct ggcattagat ttgaggcaga aaaatgaaaa
    32821 ccaaactacc gactgaatgg caagaactga gtgaccaact cggtttccaa gaattcaccc
    32881 ccattcaaac tcaactattt gagccccttc ttgctggaga aaacctccta ggagtgagcc
    32941 aaaccggaac tggtaagacc ctagcgtatc tcctaccaag tcttctcaga ctacaaaaga
    33001 aaaaagccca acaactcttg attctagcac caaacacgga acttgctgga cagatttttg
    33061 atgtatgtaa aacgtgggca gaagctatcg gattgactgc tcaactcttc ctttcaggtt
    33121 caagtcagaa acgacaaatt gaacgtctta aaaaggggcc agaaattctg attggaactc
    33181 ctggccgtat ctttgaactc attaaactta aaaaaatcaa gatgatgaat gtggaaacca
    33241 tcatcctgga tgaatttgac caattactag atgattctca gattcacttt gtagaaaaaa
    33301 tcactcacta cgcacctcgt gaccaccaac tggtctacat gagtgccacg accaagtttg
    33361 atcaagaaaa gattgtgccg aatacacgca ctattgatct ctctgatcaa aaattggaca
    33421 atatccagca tttctacatg caggtcgacc aacgtcatcg agtggatata ctacgaaaac
    33481 tggctcatgt agaggatttc cgtggtctag tcttcttcaa tagcctgtca gaccttggga
    33541 atgccgagga aaaactacag tatcgtgata tattggctgt ttccctcgct agtgatgtta
    33601 atgttaaatt tagaaaagtc atcttagaaa agtttaaaga taaccaacta accctgcttc
    33661 ttgcaactga ccttttggct cgtggaattg atatcgatag cctagaatgt gtcgtaaact
    33721 ttgatattcc tagagatagc gaaacctaca cacaccgtgc tggccgtact ggccgtatgg
    33781 gcaaggaagg atatgttatt actctcgtca ctcatccaga agaaatcaaa aaacttaaaa
    33841 agtttgcaag tatccgagaa attgtcctaa aaaaccaaga actctatatc aaataaagcc
    33901 ccattgctct atacacatcc agtgtatgga gcttttttat aagcaattca attttatact
    33961 catctgcttt caaaaagcat tctagtccat ctctgattaa cgatggactt tatcacctcc
    34021 ttctccagtc cttgtatgac atcttgaagt tgattcatga catcttccaa agttcgaaag
    34081 gctttattct taaatccacg tttacgaatc tctttccac
//
LOCUS       DBEROU010000021        33973 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.021, whole genome shotgun sequence.
ACCESSION   DBEROU010000021 DBEROU010000000
VERSION     DBEROU010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33973
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.021"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            185..445
                     /locus_tag="ACL8CL_001071"
     CDS             185..445
                     /locus_tag="ACL8CL_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330329.1"
                     /translation="MTGVSNPTTDSARLVLAEAKKAFADDSLTEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
     gene            399..620
                     /locus_tag="ACL8CL_001072"
     CDS             399..620
                     /locus_tag="ACL8CL_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330330.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQKELQKAVLF
                     LADEKGSEHTEAEVIDNLKEVIAKLKANA"
     gene            939..1868
                     /locus_tag="ACL8CL_001073"
     CDS             939..1868
                     /locus_tag="ACL8CL_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1330331.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            1882..2805
                     /locus_tag="ACL8CL_001074"
     CDS             1882..2805
                     /locus_tag="ACL8CL_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1330332.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            2889..4364
                     /locus_tag="ACL8CL_001075"
     CDS             2889..4364
                     /locus_tag="ACL8CL_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1330333.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKPVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEVLNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            4636..5622
                     /locus_tag="ACL8CL_001076"
     CDS             4636..5622
                     /locus_tag="ACL8CL_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HGS1330334.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMENLWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            5744..6604
                     /locus_tag="ACL8CL_001077"
     CDS             5744..6604
                     /locus_tag="ACL8CL_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 family protein"
                     /protein_id="HGS1330335.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDLPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIYLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQLEIQPILG
                     QDFKTLAQEK"
     gene            complement(6676..7740)
                     /locus_tag="ACL8CL_001078"
     CDS             complement(6676..7740)
                     /locus_tag="ACL8CL_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6574 domain-containing protein"
                     /protein_id="HGS1330336.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEEQAQEVADKAEETIADLDTPIEK
                     NTQLEEEVSQAEVELESQQEEKIEAPEDSEARTKIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPYQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIISILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(7803..8714)
                     /locus_tag="ACL8CL_001079"
     CDS             complement(7803..8714)
                     /locus_tag="ACL8CL_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HGS1330337.1"
                     /translation="MRKWTKGFLIFGVVTTIIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSAHHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            complement(8707..9300)
                     /locus_tag="ACL8CL_001080"
     CDS             complement(8707..9300)
                     /locus_tag="ACL8CL_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HGS1330338.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTILAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVARFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            complement(9287..9613)
                     /locus_tag="ACL8CL_001081"
     CDS             complement(9287..9613)
                     /locus_tag="ACL8CL_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HGS1330339.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGS
                     IRHDKN"
     gene            complement(9752..10918)
                     /locus_tag="ACL8CL_001082"
     CDS             complement(9752..10918)
                     /locus_tag="ACL8CL_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HGS1330340.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLTIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPAVAALLLIYILMTVALFTVDRADKIL"
     gene            10976..12136
                     /locus_tag="ACL8CL_001083"
     CDS             10976..12136
                     /locus_tag="ACL8CL_001083"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1330341.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTESFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREHGYKIRYSPSILSYQYIRPTFKKMLHQKYSNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPVTFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGLGTIVGLIRGFKWKKEYKRTIIYLDKISQINQNMLQ"
     gene            12175..14025
                     /locus_tag="ACL8CL_001084"
     CDS             12175..14025
                     /locus_tag="ACL8CL_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HGS1330342.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(14111..14458)
                     /locus_tag="ACL8CL_001085"
     CDS             complement(14111..14458)
                     /locus_tag="ACL8CL_001085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330343.1"
                     /translation="MKIKEQTRKLAAGCSKHCFKIVDGTDEVSSKHCFKVVDGTDEVS
                     SKHCFEVADRTDEVSSKHCFKVVDGTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDE
                     VSNHIRQGDVDVI"
     gene            complement(14508..15140)
                     /locus_tag="ACL8CL_001086"
     CDS             complement(14508..15140)
                     /locus_tag="ACL8CL_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HGS1330344.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(15162..16034)
                     /gene="sdaAA"
                     /locus_tag="ACL8CL_001087"
     CDS             complement(15162..16034)
                     /gene="sdaAA"
                     /locus_tag="ACL8CL_001087"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HGS1330345.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIENGKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            complement(16043..16714)
                     /gene="sdaAB"
                     /locus_tag="ACL8CL_001088"
     CDS             complement(16043..16714)
                     /gene="sdaAB"
                     /locus_tag="ACL8CL_001088"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006565 - L-serine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HGS1330346.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            16956..17543
                     /locus_tag="ACL8CL_001089"
     CDS             16956..17543
                     /locus_tag="ACL8CL_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HGS1330347.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
     gene            complement(17595..17921)
                     /locus_tag="ACL8CL_001090"
                     /pseudo
     CDS             complement(17595..17921)
                     /locus_tag="ACL8CL_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            18367..18618
                     /locus_tag="ACL8CL_001091"
     CDS             18367..18618
                     /locus_tag="ACL8CL_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HGS1330348.1"
                     /translation="MVCSILASTSVLAVWVDGGQWNYGVGWSGNFGYSDYLHSTRSHT
                     ATVKDGNKFSKDRAEAEAWARASLTKIPPTGMEYFYGFE"
     gene            18670..20778
                     /locus_tag="ACL8CL_001092"
     CDS             18670..20778
                     /locus_tag="ACL8CL_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HGS1330349.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLIFNYVMKIGTTILLTAL
                     LIGVGRSLEQENKELAYQQQWVSQGNYLTLETFKLNDNLWQEELAGSGKSTDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRNFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVTTFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSFPLLVLIFFAHAFLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            20775..21416
                     /locus_tag="ACL8CL_001093"
     CDS             20775..21416
                     /locus_tag="ACL8CL_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330350.1"
                     /translation="MIELKNITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLPKKDKLKKVEEILERVGLSATLHQRVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRVIELGEGK"
     gene            21622..22428
                     /locus_tag="ACL8CL_001094"
     CDS             21622..22428
                     /locus_tag="ACL8CL_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1330351.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQKGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKESGELDKLIEDAFKASIEK"
     gene            22445..22837
                     /locus_tag="ACL8CL_001095"
                     /pseudo
     CDS             22445..22837
                     /locus_tag="ACL8CL_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269502.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
     gene            complement(23153..23479)
                     /locus_tag="ACL8CL_001096"
                     /pseudo
     CDS             complement(23153..23479)
                     /locus_tag="ACL8CL_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            24230..24601
                     /locus_tag="ACL8CL_001097"
     CDS             24230..24601
                     /locus_tag="ACL8CL_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0218 family bacteriocin-like peptide"
                     /protein_id="HGS1330352.1"
                     /translation="MELVLPNNYVVIDEEEMMYLDGGAIYIPRWAITGAITGAAYAAL
                     AAAGGGGLQLVLASYGLRSALVAGIVKGLGVLGIHIGNAFANTVIRSIASAGIGAGAD
                     WIFTNIIDGWDGRRDNQLRIG"
     gene            24609..24821
                     /locus_tag="ACL8CL_001098"
     CDS             24609..24821
                     /locus_tag="ACL8CL_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330353.1"
                     /translation="MLIVLISVIVAFLYHKITEYVNNRKIDIVIFLSLLISHFIWNYY
                     FLPIEILIAISGANLLLSFLKKLQTK"
     gene            25195..25320
                     /locus_tag="ACL8CL_001099"
     CDS             25195..25320
                     /locus_tag="ACL8CL_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330354.1"
                     /translation="MATITNALNIAATVAEVFSLGGAIAYGLDIVDGKFDGYLWA"
     gene            25301..25547
                     /locus_tag="ACL8CL_001100"
                     /pseudo
     CDS             25301..25547
                     /locus_tag="ACL8CL_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513491.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            25704..26762
                     /locus_tag="ACL8CL_001101"
     CDS             25704..26762
                     /locus_tag="ACL8CL_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330355.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFIFSYILLFAQ
                     SYLGGNTAWIGNRRLIIDDEFEKILLSKSYIKEISSARYSEYLTCEYKNFTPIILLAI
                     FENLLDSYLLQNQSEVDLDIFYKVLPLLYKEKYTMGFNYFVSLNYLLYKVGFLGIIYD
                     NEALRDLSKQYLNKNISELQDGSFEGGIQDAVASKQIVVINEFIACLNSRCMPSQYDR
                     FFYKDRPYIFSRKSPIKG"
     gene            27077..28054
                     /locus_tag="ACL8CL_001102"
     CDS             27077..28054
                     /locus_tag="ACL8CL_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330356.1"
                     /translation="MKNKRYFFDTILMILLLISTIFCVSPVFIKLDILGTPSHAILTF
                     VLAIPLFYILSQCLHTLLLLVSSIFCKLRPIYFYFIFVIIIGARKYYRILFHQLMGFS
                     PGVAVFYKESQTTKNLFKFYYFLYFTTLISYYFFFTFVYDKPLLLPLIPFSIIIALVQ
                     KLYRIENQQLFLLKSKVLTILESKKNCEFNLQDYHEIWKLQSKSELPCVALSYISLIK
                     PYLSESVREQIDLLEVKRFKKINHPISLYGMLDVIKLNLYLRHYNEKNKYESMLKKIL
                     EVRPDFVLIEQNIDDSLNSSQPLSLSLAISEIQLLLEVYMGIKHVSIRR"
     gene            28431..28805
                     /locus_tag="ACL8CL_001103"
     CDS             28431..28805
                     /locus_tag="ACL8CL_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPH_0224 family bacteriocin-like peptide"
                     /protein_id="HGS1330357.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGFSILRWPVATAINIAFNGVLG
                     GGAISLVRNYIRNYGLRRVTSAIAGAAARYVGVRVANRVAGFALSAINGFAAWMSIGD
                     AITTIWANNDVNRRDPNLNALW"
     gene            28812..28994
                     /locus_tag="ACL8CL_001104"
     CDS             28812..28994
                     /locus_tag="ACL8CL_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330358.1"
                     /translation="MGIKMEKIFVIIFFVCLFISSITFLAYDFVSEEIKKLIIWMNVV
                     FLILIIAMIIYPKLRK"
     gene            29013..29915
                     /locus_tag="ACL8CL_001105"
     CDS             29013..29915
                     /locus_tag="ACL8CL_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330359.1"
                     /translation="MIRKLIIFLLMLPLFVLWLELHLLVSNLQLNLEIPLDFVVSTSL
                     AFCSLILSKIVLDILYALKDLYKKEALITIFPFIFIGRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIMLTTIFFWTIMDINHLVFNKTEFLFNQN
                     KWEKEDSFESDLTKTLKDKIQKSELSYSDLMSLLLYDAMNQSTFLTDSELFEDILKKI
                     EDSHNTLLCTGLVELLLYEISISNNNNWSNKVDKIRIQLIRINQLDFFYYTSWLRQNF
                     DFCMNREYHKMKSRKLLLSNKKIV"
     gene            29971..30210
                     /locus_tag="ACL8CL_001106"
     CDS             29971..30210
                     /locus_tag="ACL8CL_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HGS1330360.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAAAGVLATA
                     AGKIAYCIGYGVLNRGCDINGNPYPWDGFISATVR"
     gene            30207..30935
                     /locus_tag="ACL8CL_001107"
     CDS             30207..30935
                     /locus_tag="ACL8CL_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330361.1"
                     /translation="MSNVDKIRKIHIIVCWVYIFLSFRAIINDTEYFLLIFLAFIYSI
                     VSLPLYSVKNKIVSICLAINSILLISFPILINKFFPESFLTYTVLISVFILELAIFHL
                     IGEDFATKLTNEYKKISQFRSKVSQSPWIKYLEISSFILTIFPSILYGTVDNRVLALI
                     FLIKICVDTTIKFLFIRLFDTSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSV
                     LLLFSNFSVPFIKAKEYELFKNSK"
     gene            31414..31611
                     /locus_tag="ACL8CL_001108"
     CDS             31414..31611
                     /locus_tag="ACL8CL_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330362.1"
                     /translation="MKHFFAGIGEIKLVGYLLYICVGLAPLFHIYVIGSELSFVKIVL
                     SILGVIFVSMLTIARIYRTFL"
     gene            32068..32307
                     /locus_tag="ACL8CL_001109"
     CDS             32068..32307
                     /locus_tag="ACL8CL_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330363.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     DLYKITRDYASQTKPAIQAGVFILEDFRNILCTMM"
     gene            32292..32570
                     /locus_tag="ACL8CL_001110"
     CDS             32292..32570
                     /locus_tag="ACL8CL_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330364.1"
                     /translation="MYNDVIERISLYEFIGDIFYSKIISCCIVARDLSKNTMKLDVIF
                     FEDKNKRSAVLGLRRDKSGVFKSVTLHFTSAKKYAKVRKTDVKEMKWL"
     gene            32856..>33973
                     /locus_tag="ACL8CL_001111"
     CDS             32856..>33973
                     /locus_tag="ACL8CL_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein"
                     /protein_id="HGS1330365.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSQPTFVRAEEAPQVVEKSSLE
                     KKYEEAKAKADTAKKDYETAKKKAEDAQKKYDEDQKKTEDKAKAVKKVDEELQKANLE
                     LQKAHVKYQKAQRELSESPDRRKSAARKKLADALSYIGEVELKQKEAEANFNTEQAKV
                     IPKETELAVTKQKAEEAKKEAEVAKKKFDKAAQEVEVAKKEVEAKELEIEKLQDEIST
                     LEQEVATAQHQVDNLKKLLAGVDPDDTEAIEAKLKKGEAELNAKQAELAKKQTELEKL
                     LDSLDPEGKTQDELDKEAAEAELDKKADELQNKVADLEKEISNLEILLGGADPEDDTA
                     ALQNKLATKKAEFEKTQKELDAALNELGPDGDEEETPAP"
BASE COUNT        10658 a         5599 c         7011 g        10705 t
ORIGIN      
        1 ttcattttac tatagaacga tttgaaggcg tttatgatat ttagctgtac gagagtcttt
       61 taaaagtgtt ttgatggttt ggatttcttc tttagttgat ttcatatcac tgttatataa
      121 tactttttga ttttagtcta gtattagtaa gcagactcaa accttagaaa atcttttgag
      181 atcattgaca ggtgtatcga atcctaccac agattcagca cgtctggttt tggcagaggc
      241 taagaaagct tttgcagatg atagtttgac agagcaaggt ttacgtgata tcttgcaaac
      301 agtcaaagat gccattgctt ccctagagtc cattaaggaa agccagtcag caactaagga
      361 tggaggacaa acagcgggca aagaaacagc aggagaagat gagtctgcaa atcaaactca
      421 agaaattagc caaggaatta agtaaacttc ttaaagactc aaaccttgaa acagtagata
      481 aggatgtcct tgaaaactct caaaaagaac ttcaaaaagc agttttgttc ttggcagatg
      541 aaaaaggctc tgagcatact gaagcagagg tgattgataa tcttaaagag gttattgcta
      601 agttgaaagc aaacgcctaa taacatatta aaaatctcca gattaggaac tatcgctgag
      661 ttccctagtc tggagatttt tcagtatccc ccgttgttgg atgtttataa cagagtgacg
      721 ccatcactgg gctaatagag ataaaataaa caattgattt aggacatttg tttgatagtg
      781 gtgataaagt tttaaatctg ctacataatt tgtggagttt tttctataat taggagttta
      841 acctaaccta caaataggag tatactaata atgtaatcgt tatcaaaagt ctaaaaaaga
      901 atttttagat ggatatcaaa ataaaaaggg aggaaattat gaaaaagttt tcaaaaacat
      961 tgagagacaa ctggatcttt ctcttgatgg ttttgccagg ggcactctgg ttgattctat
     1021 tcttttacat tccagtattt gggaacgtgg ttgccttcaa agactaccac atgaccagta
     1081 atggtttcat agatagtatc ataaatagta aatgggtcgg actcgataat ttcagattct
     1141 tatttagttc aagagacgcc tttattatca cacgaaatac tgtcctctac aatcttggct
     1201 ttatctttct aggtttagtt gtatctgtag ggattgccat tatcctcagc gagctccgtt
     1261 ctaagagaat ggtgaagatt ttccaaactt ctatgttgtt cccttacttc ttgtcttggg
     1321 ttatcatcag tttctttaca gatgccttcc taaatattga taaaggggtg ttcaatcatc
     1381 tattggaaag tcttggtctc aaagaagtca atttttacgc tgacctgggc atctggccct
     1441 atctcctact tttcctaggt atttggaaag gctttggata tagcagtgtc atgtactatg
     1501 cgacgatcat gggaattgat ccaacctact acgaagcagc gacagtggac ggagctagca
     1561 agtggcaacg tattcgcaac gtaaccattc ctcagttgac tccgcttgta actgtattga
     1621 ccatccttgc agtcggaaat atcttccgcg cagacttcgg tctcttctat caaatcccac
     1681 acaatgctgg tcagctttac aatgtaacca acgttttgga cgtatatgtc tttaatggtt
     1741 tgactcagac agcagatatc ggtatggctg cagcagccgg tctttaccaa tccgttgttg
     1801 gtttgattct ggttatccta tcaaacttgc ttgcaagacg agtcgatcca aactcagctt
     1861 tgttctagaa aggaggagaa tatggcaaaa aagaaaatta aaaaagaaaa aattgataat
     1921 gtcggcattc actccttcag taagaaagca gatatcttct tcagtatcat ttcgggtttg
     1981 attgccctct cttgtatcct accttttgta ttcgttatca ttatttcagt gacagacgag
     2041 aaaagcctcc tccaatacgg ctatagcttc ttcccatccc aatttggctt agacggtttt
     2101 gagtttttgg cacagtttaa ggataaaatc ctacaagccc tcttcatctc tgtctttgta
     2161 accgtagttg ggacattgac aaatgtcttt atcacaacaa cctatgccta cgctatttca
     2221 cggacaacct ttaagtatcg cagatttttt accatcttcg tccttctcag tatgttgttc
     2281 aacgctggtt tggtaccagg ttatatcatg gtgacccgtg tacttcagct tggggatact
     2341 gtttgggcct tgattgttcc aatgcttctc tcaccattta acatcatctt gatgcgttcc
     2401 ttcttcaaga agaccattcc agaagccatt ctagaatcgg ctcgtatcga tggtgccagt
     2461 gaagcccgga ttttcttcca aatctgtttg ccattgtcac taccaggtat cgcaaccatc
     2521 acgctcttaa cagctcttgg tttctggaat gactggttca acgcccttct ttacatcaag
     2581 agtgacaact tgtatccatt gcaatatttg ctcatgcaaa tccaacaaaa tatggactac
     2641 attgccaaag cggtcggctt gactggtcaa ctgggagttg ctctaccgaa agaaacaggt
     2701 cgtatggcca tggttgtggt tgcaaccctt ccaatcgcga ttttgtatcc atttttccaa
     2761 cgctactttg taaaaggttt gactatcggt ggtgtgaaag aatagcactt gtttccaaac
     2821 ttcccttgcg tgaagttaag atcattacat tgtttataag tttaaaaata aaaaaaggag
     2881 tttttatcat gaaaaactgg aaaaaatatg cttttgcatc tgctagtgta gtcgctttgg
     2941 ctgcaggtct tgctgcttgt ggaaatttga caggtaacag caaaaaagct gctgattcag
     3001 gtgacaaacc tgttatcaaa atgtaccaaa tcggtgacaa accagataac ttggatgaat
     3061 tgttagcaaa tgccaacaaa atcatcgaag aaaaagttgg tgccaaattg gatatccaat
     3121 accttggctg gggtgactat ggtaagaaaa tgtcagttat cacatcatct ggtgaaaact
     3181 atgatatcgc ctttgcagat aactatattg taaatgctca aaaaggtgct tatgctgact
     3241 tgacagaatt gtacaaaaaa gaaggtaaag acctttacaa ggcacttgac ccagcttaca
     3301 tcaagggtaa tactgtaaac ggtaaaattt acgctgttcc agttgcagcc aacgttgcat
     3361 catctcaaaa ctttgccttc aacggaactc tccttgccaa atatggtatc gatatttcag
     3421 gtgttacttc ttacgaaact cttgagccag tcttgaaaca aattaaagaa aaagctccag
     3481 acgtagtacc atttgctatt ggtaaagttt tcatcccatc tgataacttt gactacccag
     3541 tagcaaacgg gcttccattc gttattgatc ttgaaggcga tactactaaa gttgtaaacc
     3601 gttacgaagt gcctcgtttc aaagaacact tgaagactct tcacaaattc tatgaagctg
     3661 gctacattcc aaaagatgtc gcaacaagcg atacttcatt tgaccttcaa caagatactt
     3721 ggttcgttcg tgaagaaaca gtaggaccag ctgactacgg taacagcttg ctttcacgtg
     3781 ttgccaacaa agatatccaa atcaaaccaa ttactaactt catcaagaaa aaccaaacaa
     3841 cacaagttgc taactttgtc atctcaaaca actctaagaa caaagaaaaa tcaatggaag
     3901 tgttgaacct cttgaatacg aacccagaac tcttgaacgg tcttgtttac ggtccagaag
     3961 gcaagaactg ggaaaaaatt gaaggtaaag aaaatcgtgt tcgcgttctt gatggctaca
     4021 aaggaaacac tcacatgggt ggatggaaca ctggtaacaa ctggatcctt tacatcaacg
     4081 aaaacgttac agaccaacaa atcgaaaatt ctaagaaaga attggcagaa gctaaagaat
     4141 ctccagcgct tggatttatc ttcaatactg acaatgtgaa atctgaaatc tcagctattg
     4201 ctaacacaat gcaacaattt gatacagcta tcaacactgg tactgtagac ccagataaag
     4261 ctattccaga attgatggaa aaattgaaat ctgaaggtgc ctacgaaaaa gtattgaacg
     4321 aaatgcaaaa acaatacgat gaattcttga aaaacaaaaa ataatcagct aaattgattt
     4381 cgtgtattca ttcctaactc ctaaaataag aatcaccgct ttcctcaggt tggtctgggg
     4441 agggcagtgg tttttttggc acaaggatat agatatccat gttttctctt gtccttattt
     4501 taaccattca ggaaatttcg agaaccctct acaatttcca gtcaaataaa ttgcattcgt
     4561 tttctcaagc aggtatacta gtatggataa ataaaaaatt tagaaaattt aagaatagaa
     4621 aagagaacaa atcttatggc aaaagatatt cgtgtcttac tttactacct ctatactcca
     4681 attgaaaatg cagagcaatt tgctgcagac cacttggctt tctgtaaatc aatcggcctt
     4741 aaaggccgta tcctagtcgc tgacgaggga attaacggaa cagtttcagg tgactatgaa
     4801 acaactcaaa aatacatgga ctacgttcac agccttccag gaatggaaaa tctctggttc
     4861 aagattgacg aagaaagtga acaagccttc aagaagatgt ttgttcgcta caagaaagaa
     4921 attgtccacc ttggtttgga agacaacgac tttgacaatg acatcaaccc acttgaaaca
     4981 acaggtgctt acttgtctcc aaaagagttc aaagaagcgc ttcttgataa agataccgtt
     5041 gtccttgaca cacgtaacga ttatgagtac gacctaggac atttccgtgg ggctattcgc
     5101 ccagatattc gcaacttccg tgagttacca caatgggtcc gtgataacaa ggaaaaattc
     5161 atggacaagc gtgtcgtggt ttactgtaca ggtggcgttc gctgtgagaa attctcaggc
     5221 tggatggtcc gtgaaggcta caaagatgtc ggccaattgc acggaggaat cgcaacttac
     5281 ggtaaagacc cagaagttca aggtgagctt tgggatggga aaatgtacgt ctttgacgag
     5341 cgtattgccg ttgatgtcaa ccatgtcaac ccaaccatcg tagggaaaga ctggtttgat
     5401 ggaacaccat gtgaacgtta tgtcaactgt ggaaatccct tctgtaaccg tcgtatcttg
     5461 acatcagaag aaaatgaaga caagtacctt cgtggatgct cacacgagtg tcgtgttcac
     5521 ccacgtaacc gctatgtttc aaaaaatgaa ttgacacaag ctgaagtgat tgagcgccta
     5581 gccgctatcg gtgaaagctt ggatcaggca gctactgtat aagatcaaac agtcctcagg
     5641 ggctgttttt ctatgctttg tactcaaaaa tctaaagttt gtttttgtat ctttcaggaa
     5701 aataaggtat actgtatgta aacgatttca aaggagtcca gttatggcaa aaacattttt
     5761 tattccaaat aaacagagca ttttaggaga acaagagatt ttgaatgcca agtcgatctt
     5821 ggccttgcta gacggtttgg agtcacatag ctatgatgta gtctatcttc gtcagcctct
     5881 taatcgtctc gagtatattg agtgtgcgat agtggggcaa tcacaatttc tctttaaggt
     5941 cagttatgct gatggtcaaa aggcttaccg tgtcgatctt cctgacctac taacaaagac
     6001 agactggcag attatcaagt catttttaga tgctttgctt gcttatacag ggactgatat
     6061 tgaagggcta gatggttttg attttgaagc ttatttccaa gcaagtattc aagcctatct
     6121 agcagaccct gtagctcgtt ttacgatttg ccaaggaatt tttaatccta ttttctttag
     6181 tcgtgagaac ttgaaaagct ttttagaggc agatggcttg gctcagtttg aagcgcgtgt
     6241 gcgtgcggtt caagagacag atgcctactt tgcaagagtt tccttctatc aggatggaga
     6301 aggaaaagtg catggcgttt accatttagc tcaaggagtc aagacagttt taccgagaga
     6361 accgtttgtt cctgcagcct atattgagca attggtggat aaggaagtcc agtgggagat
     6421 ttacttggtt caaatcacag gagacggctc taaaccagaa gactatgaat ccatagctcg
     6481 cttggactat gcaaaattct tagaggtgtt acccccatct ttttaccacc agctagacgc
     6541 caatcaatta gaaatacaac ccatcctagg acaagatttt aaaacattag cacaagaaaa
     6601 gtaaagcaga agcaggtcaa tcgacttgct tttttgacat agaaaaaatc ctgccaagga
     6661 tgacaggatt gaattttaaa gaaaggccaa gatacgaaga taatctccaa tcagtgccac
     6721 ttcagctaca aagaagagga ggataataac tccgttcaca aggacagaca agaataattg
     6781 atagaaggag tcggtttcac ttgcttgact tggtcttgta atgatatgga gactggcaag
     6841 cagaatgatt ccaatgctaa ttacacacaa gagagctgta aatcgtaggc tatcaaagaa
     6901 agcaaagaaa ctagcaatag cagtgaggaa gattggaatt gccaagagtt gactatattg
     6961 ttggagaacc ttgtctagcg tccagtcctt ttcctggtgg ataaatcgtc tcacaacgaa
     7021 actacccaag aggaatgaaa agaagaagag tgttgtcgct actaggatag agatgataga
     7081 aaaaagagtt aaaggagcta gctgctcagg gaagcgactg ttaatgcttg ctatatgtcc
     7141 atagtaagca tgtttgatgt gatagatact aaagaaaaag gaagatgcag aaaacagaat
     7201 gagcaagaga aaggctgtgt aactgtgtgt gatacttgtt tccaacttac ttgtaggaga
     7261 tttgatcgct tccactagcc aagaccaaaa atcaagcact tgctctttcc atttatccct
     7321 agattttgga gcttggtagg ggatataagg actttctaaa gatttactga taagaagtgg
     7381 ctctttcgta gttgcttttt gctgaggaag agcttcttgg ctctcttcag ctatagtgac
     7441 tttttctgtt tctttagaaa ggtctggctc ttcttcagta gaattagatg ccttcttttc
     7501 ttctattttt gttctcgctt cactgtcttc aggagcttca attttctctt cttgctggct
     7561 ttccaattcg acttcagctt gagagacttc ctcctctaac tgagtatttt tttcaattgg
     7621 tgtatcgaga tcggctatcg tttcttcagc cttgtctgca acctcttgag cttgctcttc
     7681 aggcttgttc ttgcttgttg tttttacaaa atcattactt tcaaaccatt cttgtttcat
     7741 ggtagaacct cctttttagt tagataaata tgctttcata gtagcagatg taagcgtttt
     7801 tgtcaacgtc tgcttggtgt ggatattaga tcaatattat catcagatct cgcaatgagt
     7861 tgatccttga catcggtttt ttcagttttg taagggttgc ttaattccgt acctcttgat
     7921 tcaggctttt ctcttgtgaa ttggaagata gaaccatagt tgcttgagat gtcccagtta
     7981 attcgttggc tttctttctg gtctaggatg attctgagat aatctttggc agtcagttca
     8041 accttgccat ggacttggat attttcagcg tggaagtgat tctctgttga ctctagctga
     8101 ctatctgtaa gaactgtatc aaagatatta acgatattgg gcgttgtgag tttactgttt
     8161 ttgatacgac ttccttcaat tcggaggata tagctgtttg tattgagggt cgcattttca
     8221 aggctagcat ttatgatggt ggtttgtccg cgattggctg agatgttgat cccttttaga
     8281 gttctccctt ttggtagctg gagaataact tcttcaaaac gactagagta gctacttgcg
     8341 atatgaagaa tcccaccaat tccagaagag agaaacggag tttcagacag tttcttatca
     8401 gtgagactca gagttctatc gttctgattg gtgataagat catggtgagc agaaagagat
     8461 ggatggtaag aaatgtggat ttgatcatcg aaagagtctg tgatggtgag cgcgtgttgg
     8521 tggagagtaa tttctaggtt ttcgacttcc ttgccaaagg ttagcttttc cgtacggcta
     8581 tcatagacag gttctttgga catggaaagt aggctcttaa tcccgtcaga ttggatacct
     8641 acaaaaagca ggataaagcc gataatggta gtcaccacac caaagatgag aaatcctttt
     8701 gtccatttac gcatgctgat tacctctctt tcctttttta agaacaaatt gtaccagacg
     8761 aacaatgagt agaccgaaga agcgagctac ataggaaatg ccaagtaaaa ctagcgaaga
     8821 agcaccgata gccagtaaac cagaaccaaa aatcaagata aaggctgatt tggcttgggc
     8881 gaggatagtg aaactttcaa ctaaaaatag gaatccgccg atgataccca gtatggaaac
     8941 tgcaaagaaa gccagaatga cagtcaaagc ggctacaagg attgcgaaca gggtcacgag
     9001 gatggcgatt cccagaggaa tgccgatagg tgctgcaagg agggctaaca aggcgatatg
     9061 taaaatttgt cggttatttt tttgagcggg tgcttcattg atttttttat cgagaagatt
     9121 ggatagaact tcgtgggccg cttctttggg agttcccaaa ctagcgatga gttcttcttc
     9181 tccttcgact ccagcatcgt caaagagctc tctgaaatag tccatggctt cgatacggtc
     9241 agcttcaggt agtttcttga gatagagttc tagctgagtc aggtattcag ttcttgtcat
     9301 ggcggatact cccttctatg atgccattga tggtgtctgt atagagtgtc cattcatctt
     9361 ttagggtcaa gagctgctct ataccaccgt ttgtcaagga gtagtatttg cgcatgcgac
     9421 cttggaactc tctagaatag gttgtcagaa agctattgcc ttccaatttt ttgagaatgg
     9481 gatagagtgt ggattctttg atattagcga tcagcttaat ggtttggcta atctcataac
     9541 cataagaatc accctgctcc agtacagcca agatgagaaa ttcaatcaag gcagaggatg
     9601 ttggaaagta catgggaaac ctccttttct aatgtgtaag atttttatat ataatttttc
     9661 tacacataca ttgtacatct aaaagaaagc cctgtcaaga gaaatgtgta aaatttttat
     9721 atataaaaaa cttctagcta aaactagaag tttaaaggat cttatccgct ctgtccactg
     9781 taaagagggc cacagtcatc aggatataga tgagcaagag ggcagctaca gctggtaccc
     9841 aagagtggaa caggtcaaaa ctgtaaccaa agagggttgg tccaaaggct gctaggatat
     9901 agcctcctgt ttgagatagg ccggacaatt gggctgtctt ttcaggggcg cttgtcttga
     9961 gtgaaaagtt gaccatgaga taagggaaga gggcgctggt tgcggttccg atgaggagat
    10021 ggatggtaag ccagtaaatg aaattattga ttgggaaaaa gagcatggaa atgccgacca
    10081 caccagctag tgaaaccaga gtgagcatga gctgacggtt gcgagtagat aaactggttg
    10141 tcaggcttgg gatggtcatt gaaaaaggaa tgctaatcag agataagata gaagtcagca
    10201 agccagcttc gtgactggat agacctgcat ggatagacat ggtaggtaac caggtcatga
    10261 cggtgtaaaa gatcaaggat tgaaaacctg aaaagataat aattgcccaa acctgtttat
    10321 tacgcatgac ctttatttga cttttttgtt tggtttgtgg agctagtcta tgattatagc
    10381 ggtgatttgg gagccagacc aaaaaagttg ctagacagag taacgtgagg agaaggataa
    10441 gtcctttcca agaactggct tgtgtaatgg gcacagctag ataggaagcc agagccgttg
    10501 caatccccat agacgttaca tataaggtgg tcagaaaacc aattttcttt ggttgattgg
    10561 cttggataag actaggaagc aggacattga tgactgcgac acttgcccca accatcaagg
    10621 ttcctagata gagtaggggc agattgatta gtcgaataag tgagccgatg gtcaagaaga
    10681 agaggctgta ggtgaagaga tgctccaagc cgattttctg agccagttgg gtagaaaatg
    10741 gtgaaaagag ggtaaacata aggagaggca ggctggtcaa gacaccaagc gaactaactt
    10801 ctacctctag accttgcgaa atatttccca aaataatggg taaaacagta aaaggagttc
    10861 gcaaggaaac accaatcagg ataatacctg gaacaaaaaa gagtgattgc tttttcatat
    10921 aatacctctt atagctgatt ttaataacag cttattttaa ggctttttca ctataatgtc
    10981 aagtaaggtt tctggctttc aagataaaaa tgggaaagtc ttgaaaatta tgatagaatg
    11041 gtggaaggaa aaattcagga gagtagtagt gactcaaaat gttgaaagtc ttctcgtatc
    11101 cattgtaatc agtgcataca atgaagaaaa atatctgcct ggtctaattg aagacttaaa
    11161 aaatcaaacc tatcctaaag agaatattga aattctattt ataaatgcta tgtccacaga
    11221 tgggaccaca gctatcattc agcaatttat aaaggaagat acagagttta actcaattag
    11281 attgtataac aatcctaaga aaaatcaagc tagtggtttt aacctgggag ttaaacattc
    11341 tgtaggggac cttattttaa aaattgatgc tcattcaaaa gttactgaga gttttgtaat
    11401 gaacaatgtg gctattattc aacaaggtga atttgtctgt ggggggccta gaccgacgat
    11461 tgtcgaagga aaaggaaaat gggcagagac cttgcatctt gttgaggaaa atatgtttgg
    11521 cagtagcatt gccaattatc gaaatagttc cgaggataga tatgtttctt ctatttttca
    11581 tgggatgtat aaacgagagg ttttccagaa ggttggttta gtaaatgagc aacttggccg
    11641 aactgaagat aatgatattc attatagaat tcgagaacat ggttataaaa tccgctatag
    11701 cccaagtatt ctatcttatc agtatattcg accaacattc aagaaaatgc tgcatcaaaa
    11761 gtattcaaat ggtttgtgga ttggcttgac aagtcatgtt cagtttaagt gtttatcatt
    11821 atttcactat gttccttgtt tatttgtttt gagtcttgtg tttagtctag cattgttacc
    11881 ggtcacattc gtattcataa ctttactatt aggtgcctat tttctacttt tgtcattact
    11941 cactttgctg actttattaa aacataaaaa tggatttcta attgtgatgc cctttctttt
    12001 attttccatt cactttgctt atggccttgg gacgattgta ggtttaatta gaggatttaa
    12061 atggaagaag gagtacaaga gaacaataat ttatttggat aaaataagcc aaataaatca
    12121 aaatatgcta caataacaat atagtaaaac tcttttaagg aggagtagat ttctatgaat
    12181 aaaaaactaa cagattatgt gattgatctg gtggaaattt taaataaaca acaaaagcag
    12241 gttttctggg gaatatttga tattttcagt atggtggttt ccataattgt atcttatatt
    12301 ttattttatg ggctgattaa tccagcacct gttgactaca ttatctatac gagtttggcc
    12361 ttcctgttct atcaattgat gattggtttt tgggggttga acgcgagcat tagtcgttac
    12421 agcaagatta cggatttcat gaaaatcttt tttggtgtga ctgctagcag tgtcttgtca
    12481 tatagtatct gttatgcctt cttgccactc ttctccatcc gttttatcat tctctttatc
    12541 ttgttgagta ccttcttgat tttattgcca cggattactt ggcagttaat ctactccaga
    12601 cgcaaaaaag gtagtggtga tggagaacac cgtcggacct tcttgattgg tgccggtgat
    12661 ggtggggctc tctttatgga tagttaccaa catccaacca gtgaattaga actggtcggt
    12721 attttggata aggattctaa gaaaaagggt caaaaacttg gtggtattcc tgttttgggc
    12781 tcttatgaca atctgcctga attagccaaa cgccatcaaa tcgagcgtgt catcgttgcg
    12841 attccgtcgc tggatccgtc agaatatgag cgtatcttgc agatgtgtaa taagctgggt
    12901 gtcaaatgtt acaagatgcc taaggttgaa actgttgttc agggccttca ccaagcaggt
    12961 actggcttcc aaaaaattga tattacggac cttttgggtc gtcaggaaat ccgtcttgac
    13021 gaatcgcgtc tgggtgcaga actgacaggt aagaccatct tagtcacagg agctggaggt
    13081 tcaatcggtt ctgaaatctg tcgtcaagtt agtcgcttca atcctgaacg cattgtcttg
    13141 ctcggtcatg gggaaaactc aatctacctt gtttatcatg aattgattcg taagttccaa
    13201 gggattgatt atgtacctgt gattgcggac attcaagact atgatcgttt gttgcaagtc
    13261 tttgaacagt acaaacctgc tattgtttat catgcggcag cccacaagca tgttcctatg
    13321 atggagcgca atccaaaaga agccttcaaa aacaatatcc gtggaactta caatgttgct
    13381 aaggctgttg atgaagctaa agtgtctaag atggttatga tttcgacaga taaggcagtc
    13441 aatccaccaa atgttatggg agcaaccaag cgcgtggcgg agttgattgt cactggcttt
    13501 aaccaacgta gccaatcaac ctactgtgca gttcgttttg ggaatgttct tggtagccgt
    13561 ggtagtgtca ttccagtctt tgaacgtcag attgctgaag gtgggcctgt aacggtgaca
    13621 gacttccgca tgacccgtta ctttatgacc attccagaag ctagccgtct ggttatccat
    13681 gctggtgctt atgccaaaga tggggaagtc tttatccttg atatgggcaa accagtcaag
    13741 atttatgact tggccaagaa gatggtgctt ctaagtggcc acactgaaag tgaaattcca
    13801 atcgttgaag ttggaatccg cccaggtgaa aaactctacg aagaactctt ggtatcaacc
    13861 gaactcgttg acaatcaagt tatggataag attttcgttg gtaaggttaa tgtcatgcct
    13921 ttagaatcca tcaatcaaaa gattggagag ttccgcactc tcagtggaga tgagttgaag
    13981 caagctatta tcgcctttgc taatcaaaca acccacattg aataaaaaag aaaaacgcat
    14041 attatcaagt taaacaacct tggtaatatg cgttttatta tgtagagact tatactcttc
    14101 gaaaatctct tcaaatcacg tcaacgtcgc cttgccgtat atggttactg acttcgtcag
    14161 ttctatccac aacctcaaaa cagtgttttg agctgacttc gtcagttcta tccacaacct
    14221 caaaacagtg ttttgagctg acttcgtcag ttccatctac aaccttaaaa cagtgttttg
    14281 agctgacttc gtcagttcta tctgcaacct caaaacagtg ttttgagctg acttcgtcag
    14341 ttccatccac aaccttaaaa cagtgttttg agctgacttc gtcagttcca tctacaatct
    14401 taaaacagtg ttttgagcag cccgcagcta gtttcctagt ttgctctttg attttcattg
    14461 agtattactt cattttcttc tgaaatggag ttgttaccca gtctatgcta ttgaaaatac
    14521 gccaaaactt ctaagggttt gtgagcgata taatcaggtt gatagtttag tagatctgct
    14581 tgctctccaa atccccaagt gacggccaat ttctgaatac ctgtttctcg agctcccagc
    14641 atatcaaact tggtatctcc gatgatgatg gcttgttctg gtgctagttg atgtgtctgc
    14701 aaggcttggt gaatgacatc tgccttatgg ggtgcttcag ggctagaacc ataaatacca
    14761 tcaaagaaat gatggatttc caagtttttt gccatgtctt gagcagtaga tgtattcttt
    14821 gtcgtggtga tgtagagtgg ataactgctc gataactcct caagcaagtc tataatctga
    14881 ggaaagagtt gagcttcata gatgcctttt gccttatagt aagaacgata tatctgcatg
    14941 gcttcagaaa tttggtcttt ggacaggcag gtcgcaaaac tactttcgag aggtggtccc
    15001 ataaaaccac gaatagtttt ggcatcaggg ctaggcaccc ccagctcttt aaaggtatag
    15061 gtaaaggcat tgtgaatccc gatagaacta tcaacgaggg ttccatccaa atcgaaaaaa
    15121 atcgctgtga tagaggtcat ggtttctcct atttgataag cttattctcc gaaaatttct
    15181 ttttggaggc gacgaccagt aggggtggta gcgagtccac cttcagctgt ttcacgaaag
    15241 gcagttggca tgcttgctcc tacttggtac atggcatcga tcacttcatc cacagggatt
    15301 ttagattcga tacctgccaa ggccatgtct gctgcgatga aagcaaagct agctcccatg
    15361 gcattacgtt tgacacaggg aacttcgacc aaacctgcaa cagggtcaca gatgaggcct
    15421 agcatatttt taatgacaaa ggcaatagct tgactggcct gataaggtgt tccacctgca
    15481 gccagagtca aggcggcagc actcatagca gaggctgaac caacttcagc ttgacaccca
    15541 ccctcagcac ctgagatgga ggcattgttt gcgatgacta gtccaaaggc accagcagca
    15601 aagaggaaat ccaattgttg ctcgtggctg aggtctaatt tttcaatagc agcagtgaga
    15661 acggatggca gacagccagc acttccagcg gttggagtgg cacagaccaa gcccattttg
    15721 gcattgtgtt cattgactgc gatggcattt cgggcagccg agagaatcgt ataatctgac
    15781 agagtttttc cgttttcgat gtagtgatcc aatttggcag catctccacc tgtcaggcca
    15841 ctacgagatt tattttcatt gaggccaagt tggacagagg ctttcataac ttccagattg
    15901 cgttccatga gaaggaagac ttcttcacgt tcgcgaccgg tcaattcaaa ctctgttgta
    15961 atcatgagtt ctgcgacatt tccttgaaag tccagatctg cttgctcgac caattctttg
    16021 atagaataaa acatgcttcc tcctatttaa agaaattgac attgtggaga tgagggattt
    16081 ttcgaatttc ttcgatagcc tcatcacagt tgcgactgtc aacttcgata atcataatgg
    16141 ctttttcacc agctttttca cgagtgacat tcatctgggc gatattgata ccatagcggg
    16201 aaagcgcctc tgtaacaagg gcaatcatac ctggaatatc ttgatgaacg atgatgatag
    16261 tcggtgtatt catattgaga gagacggcaa aaccattgag ttcggttacc tgaatatttc
    16321 ctccaccgat agaaatacca gtcacgctga tggtcttgtg ggcattttta acagtaattt
    16381 tagtggtgtt agggtgaggg gcattgctgt ctttctgaat ggtccagaca atcttgatac
    16441 cacgcttgtg ggcaatttcc agactatttg gaatttcagg atcatctgta tccattccta
    16501 aaatacctgc aacaagggct aggtctgttc cgtgaccacg ataggtcttg gcaaatgagt
    16561 taaaaagttg gaattcaact tctgtcggag tatcatcaaa aatggaagag acaatcttcc
    16621 caatacgaac agcaccagcg gtatggctac tagatgggcc aatcataact ggtccgatga
    16681 tatcaaagac agattgaaaa cgaagtgatt tcatcagttt ccccttataa aaattcttat
    16741 ctctattata tcaaagaatg aggggcttgg ctttaattgt ggatgaaaac ctttctaata
    16801 cctcaaatag cataaaaata gtatctttta tgacaaaaaa caccttattt agggaaataa
    16861 aaaataattt tgtaatattt ctacataaaa gtgtcaagaa acggtaatat ttaaagggta
    16921 tgatagaact atagaaagaa ggagaatttt cgaatatgaa atcaataact aaaaagatta
    16981 aagcaactct tgcaggagta gctgccttgt ttgcagtatt tgctccatca tttgtatctg
    17041 ctcaagaatc atcaacttac actgttaaag aaggtgatac actttcagaa atcgctgaaa
    17101 ctcacaacac aacagttgaa aaattggcag aaaacaacca cattgataac attcatttga
    17161 tttatgttga tcaagagttg gttatcgatg gccctgtagc gcctgttgca acaccagcgc
    17221 cagctactta tgcggcacca gccgctcaag atgaaactgt ttcagctcca gtggcagaaa
    17281 ctccagtagt aagtgaaaca gttgtttcaa ctgtaagcgg atctgaagca gaagccaaag
    17341 aatggatcgc tcaaaaagaa tcaggtggta gctatacagc tacaaatgga cgttatatcg
    17401 gacgttacca attaacagat tcatacctga acggtgacta ctcagctgaa aaccaagaac
    17461 gtgtagcaga tgcctacgtt gcaggacgtt acggttcatg gacagctgct aaaaacttct
    17521 ggcttaacaa tggctggtat taagaactaa cgaacaaata atatgtaaag agtcgaggaa
    17581 atccctcggc ttttttatct ttccttcgct tggtaataga tctctacttc ttttttgaga
    17641 tgagacagca tagaagtttc ctcggttagc tctaggagtg agaaaccttt ttggataagt
    17701 ttcgcatcat ccctacaaat cccaataggg acatagcaga tagccagcct atcgtagctt
    17761 ttcttattct tggcgtcctc cagtaaagtg tagcagattt gcttacacat gtttttatct
    17821 tgattgtata gatagagtgt ggaaaggttg agcaggattg ctattcgcaa gtcatataaa
    17881 tgttgatagt ttttatagac ttctaagcgt tgcaagattt tctcggatta ttgacaaata
    17941 aacggacatt ctgatagacg attgccctag cggcttattg taaactttgc aaatttggct
    18001 tgtaattttt tttataaaat tcacttcgat tacttgacag ttattatatt ttcacttatt
    18061 atatagataa aaggatggga taagagttta aaagtttgaa agcgctatcc atattgttta
    18121 ttgggctgat tcttgagtaa tagttagagt gtgattaata ctactctggt tgttcaaaga
    18181 cctgatacag aagagaacta ttctcctagt taagagtaag ttcgttgttt aaaaatgaaa
    18241 tgagatagaa ggaaggtgag tccaatggac tacgatactt gttgacaaat tactatagct
    18301 gtttctacgg aatagctctc gataatagga gaaataaatc atgagaaaat tagtgttgca
    18361 tctttaatgg tatgtagtat acttgcttct acaagtgttt tggcagtatg ggttgatggt
    18421 ggtcaatgga actatggagt aggatggtca ggaaattttg gttattctga ttatttacat
    18481 tctactcgct ctcatacagc aactgttaaa gatggaaata aattctctaa ggatcgtgca
    18541 gaagctgagg catgggctag agcttccctc accaagattc cgccaacagg aatggaatat
    18601 ttctatggat ttgagtaaaa ttcttccctc tcctatttgg agagggaaaa ctgtagtgga
    18661 ggcactttta tgaaaaaaat cagtaatttc tgtatgttac tcctgcttct gtgtaccact
    18721 ttttttgttt ttaatgtaaa ctatacacga gaagtggttc ggattcaaga aatgggaaag
    18781 actgtagatt ctttggattt gtatttgaaa gatattaacg aacctgcagc gtctgttctt
    18841 cgattttttg aggatgtatc aaaggagtat aaagtctcca tcatcaaaac agacagtggt
    18901 gatgaggtgg tcaagtctgg tgtttttgat aaagatacct tcccctacca agagtttggg
    18961 atttcttctc ttgattttac cacagatggt gaaggagtct atagtaataa agaaatttcc
    19021 aataaacttg gtacgattcc gacctttcta aaagccaaac ctattcagct tatgactttt
    19081 caaacctata tcaaggatac atctcgtagt ttaaatggtc gctatacgat aacttctaca
    19141 caagagatgg acaaggatag gattgtacag aaatggagcg attttttcaa gatagaccag
    19201 gctaccttgc tagagccgac ctacaaaagt gcagtggaag tcataaatcg agatttgctt
    19261 ttatctgcca ttgtttttgt cttggctatt ttgcttcttg tgttagtgac agtgtatcaa
    19321 ccgatgatgg agatgaaaag agttggggta caaaaattac ttggttttca agatagggct
    19381 gttttagctg atgttgtaaa aggcaacctt tacctcctcc taggtggggc tcttgtgatc
    19441 aatctaggcg tgtttttctt gcttgattat aagccaaaag atttgtttcc tatgctgtgg
    19501 ttgtctcatt ttttgctgtt gcagctttat ctctttatca gttggttgac ttacctctta
    19561 atccaaaaaa tgacaatcag ctctctgctg aaaggttttt catctttcaa atttggtctt
    19621 atcttcaatt atgtgatgaa aatagggaca actattttac tgacggcctt actgattggg
    19681 gtgggcagaa gtttagaaca agaaaacaaa gaacttgctt atcagcaaca gtgggtaagt
    19741 caaggtaatt acctgacctt agaaaccttc aaactcaatg ataatctgtg gcaagaagag
    19801 ctagcagggt cagggaaatc tacagattat ttctatcgat tttatcagga tttggtagaa
    19861 aaaacgcagg cgggctatgt gcagagtagc agtcttcctg taaaaaattt tgtccaatca
    19921 gaacagattc agcaatatca gttaacagat acggtggatg tttactatgc caatcgcaat
    19981 tttctaaaga gcaagggatt caagctacca gataccggta ttaaaaaagt tattttgatg
    20041 ccagcaagta cgaaaggtga agaagataaa aatcagctct tggggaagtt aattgccttt
    20101 cattcgatga agtatgaaga gcagcaaaaa cgaacgatag aggagatgga tgtcgagatt
    20161 gcctattatg aaggagattg gtcatttttc ccatatagtg ataagcgaaa ggaaaatctc
    20221 tccaatccaa ttattagctt ggtcaatgat tctgatatga tgtgggatga gaaagcctcc
    20281 ctgtcaacaa ctggcttaaa taatccgatt aaaattgaaa atacggttca acatcaaaaa
    20341 gagattacag agttagttga gaaattgtca gatggaaatt atttaaaatt ttcatctatt
    20401 caagccattc aacaagagaa agtggattct tatagagatg ctgttcggaa ttttaaccta
    20461 ctctttgctt tgtttggtct ccttagcatg atgatttcct acttcttact agtaacaact
    20521 ttcttattga agcgcaggga tatcattacc aagaagttta tggggtggaa actggtcgat
    20581 cgctaccgtc ctctcctcgt tctgctcttg ctgggctata gtttccctct tctagtcttg
    20641 attttctttg cccatgcgtt cttaccactt ctactgtttg caggttttac atgtctggat
    20701 atactatttg tgctaggctt agcttctagg atggagaaaa gaagtctagt agagttattg
    20761 aaagggggca tcttatgatt gagttgaaaa atattaccaa aaccattggg ggaaaagtga
    20821 ttttggataa cttatctctc aggattgatc agggggattt ggtagctatt gttggtaaga
    20881 gtggtagtgg gaagtcgacc ttgttaaatt tattgggttt gatagatggt gattatagcg
    20941 gacggtatga gatttttggt cagacaaatc tagcggttaa ttctgctaag tcgcaaacaa
    21001 taatccgtga acatatctct tatctgtttc aaaattttgc cctgattgat gatgaaacgg
    21061 tcgagtacaa tctcatgctg gcgctgaaat atgtgaaatt gcctaagaaa gacaagctca
    21121 aaaaggtgga agagatttta gagagagtag gtttgtcagc tactttgcat caaagggtct
    21181 ccgagttgtc tgggggcgaa caacaacgaa ttgcagttgc tagagccatc ttaaaaccca
    21241 gccagctgat tttagccgat gaacctacag gttcgctgga tcctgaaaat agagatttgg
    21301 tcttgaagtt tctcttagag atgaatcgag aagggaaaac agtcattatt gtgacccacg
    21361 atgcttatgt agcccaacaa tgtcatcgtg tcattgaatt gggcgaggga aaatgagttc
    21421 attcagctcc ttttgactgg ctgaatactc atgttttcca gagaaaaatg gcataaatac
    21481 gcctaggaat gacattttat gtagtatttc taggtttttt tgtttcaaat tgaaaatttt
    21541 ttcaatttag gcttgacaaa ggatgagtat aggagtatta tttatacaat aaaaaagaat
    21601 aaacataaag gaggctttgt tatgaataag atgaagaagg tgttgatgac gatgtttggt
    21661 ttagtgatgc tccccctact atttgcttgt agtaacaatc aatcggctgg aattgaagcc
    21721 atcaagtcca aaggaaaatt ggttgtagcc ctcaatccag attttgctcc atttgaatat
    21781 caaaaagtgg ttgatgggaa aaatcagatt gtgggttcag atatcgaatt agccaaggct
    21841 atcgcaacag aactaggtgt cgaattggaa ctatctccta tgagttttga caatgtactg
    21901 gctagcgttc aatcaggaaa agctgacctt gccatatcag gtgtttctaa gacagatgaa
    21961 cggagcaagg tgtttgattt ttcaactccc tactatactg caaaaaataa actcattgtc
    22021 aaaaaatctg atttagccac ttatcagtct gtcaacgatt tggcgcagaa aaaggtcgga
    22081 gcgcagaaag gttcgattca agagacgatg gcgaaagatt tgctacaaaa ttcttccctc
    22141 gtatctctgc ctaaaaatgg gaatttaatc acagatttaa aatcagggca agtggatgcc
    22201 gttatttttg aagaacccgt tgccaaggga tttgtggaaa ataatcctga tttagcaatc
    22261 gcagacctca attttgaaaa agagcaagat gattcctatg cggtagccat gaaaaaagat
    22321 agcaaggaat tgaaagaggc agtcgataaa accattcaaa agttgaagga gtctggggaa
    22381 ttagacaaac tcattgagga tgcctttaaa gcgtccattg aaaaatagaa agaaggaaaa
    22441 gaacatgagt aaagaaaaag taattttggc ctattctggc ggtttagaca catcagttgc
    22501 tattacctgg ctgaagaaag actatgatgt tgttgcagtt tgtatggatg tgggtgaagg
    22561 gaaagactta gatttcatcc atgataaggc tcttaaggtt ggggcagtcg aatcttatgt
    22621 cattgatgtt aaggacgaat ttgctacaga ttatgtgcta gtagctctcc agtcacatgc
    22681 ctactatgaa caaaagtacc ccttggtatc tgccttgagc cgccctctta tttctaaaaa
    22741 attggttgaa atagcgcatc agacaggagc gactacaatt gctcatggtt gtacaggtaa
    22801 ggggaatgac caagtcgaat accagattgc agtcgctaag aaagcgaatg aagctaaaaa
    22861 gtgatggagt gatgggttca gaataagagg gtggtcaatt ggcagcctct ttcttgtcgt
    22921 tgaaaaagtg agatatattg acttttgaaa aaaatgtcat aattgagttg ggataaacgt
    22981 aaaagtcata ctttctcgtt aaattgttcg gcgattcatg gtgacatggc cacatagtag
    23041 taaacacact atgtggcttg gcagagctaa aaacttctgg tttaacaatg gctggtatta
    23101 agaactaacg aacaaataat atgtaaagag tcgaggaaat ccctcggctt ttttatcttt
    23161 ccttcgcttg gtaatagatc tctacttctt ttttgagatg agacagcata gaagtttcct
    23221 cggtcagctc taggagtgag aacccttttt ggataagttt cgcattatcc ctacaaatcc
    23281 caatgcggac atagcagata gccagcctat cgtagctttt cttattcttg gcgtcctcca
    23341 gtaaagtgta gcagatttgc ttacacatgt ttttatcttg attgtataga tagagtgtgg
    23401 aaaggtttaa tagaattgtc attcgcaagt catataaatg ttgatagttt ttatagactt
    23461 ctaagcgttg caagattttc tcggattatt gacaaataaa cggacattct gatagacgat
    23521 tgctctagcg gcttagtgta aactttacaa atttggcttg taattttttt ataaaattca
    23581 ctttagttac ttgacagtta ttatattttc gctcataata tagataaaag gatgggatag
    23641 gagtttaaaa atttgaaagc gctatccata atcgtaacgc ataattttct ccttttcgtg
    23701 aaaaaagtta acgtaatttt gaaatgagct taataagagg tactatgtag gtagaaaata
    23761 aagataggag aaaaagtatg aataaaaaaa tattttgtat tctagtatgt attatatcat
    23821 tgattagtct agctatagtc tttccatggg gctggtcgat ataatgtaag ggagcttgag
    23881 ttgttagtgg aggataaaat gttaagagta atagtagtta ttcaatcttt agtaataata
    23941 ctgcttgctg ttgctttatt attatctcgg aaaaactttg attcgttaaa tgttgttctt
    24001 ataatgatga tcgtatcaca attgttgtta ttagtatatt tgataaaatc agggaatcag
    24061 tgattagctg ttatttgaat taagtaaact gttaagcttc aagcagtaac tggttttaaa
    24121 tatttaaaat aagataaagg aaggaagtct aatttaggta actaaacaaa gcctgcgatc
    24181 acttgttagg aaaccatcct aagcatgctt aataaaaagg agaaaacaaa tggaacttgt
    24241 attaccaaat aattatgttg tgattgatga agaagagatg atgtaccttg atgggggggc
    24301 tatatatata cccaggtggg caattacagg agccattact ggtgcagcat atgcagcatt
    24361 agcagcagca ggaggtggag gccttcaact agttcttgca tcttatggat tacgctccgc
    24421 actggtagct gggattgtta aaggtttagg agtattagga attcatattg gaaatgcttt
    24481 tgcaaatact gttattagaa gtattgcatc tgctggaatt ggtgctggag ctgattggat
    24541 ttttaccaat attattgatg gctgggatgg gcgacgtgat aatcaattga gaataggtta
    24601 ataatcttat gttgattgtt ttaatttcag ttattgttgc attcctgtat cataaaatta
    24661 cagagtatgt caacaacagg aaaattgata ttgttatttt tctatcattg ctgatttctc
    24721 attttatttg gaattactat tttttaccaa ttgaaatatt gatagcaatc agtggagcaa
    24781 atttactatt aagtttttta aaaaaattac agactaaata atattaatct gtgtaataca
    24841 gtagctcggt cattagattc tattgaagat ataaattaaa aatgaggctg ggaaaaaact
    24901 caatttgaaa atgagattga gtaaatcttt ctatctatat aacgtatatc atcaatattt
    24961 ttgaacacct gataatatgc atttttctta ttttaaagat tttttgcctt tcttcatttc
    25021 ttgaatgaat tggagagatg actttattta ggtgtttgac attactatag ataggattta
    25081 gcagtactaa tatgaactca tattataggt gcagacttaa attttggaag caagctaaag
    25141 gatatcttat ctaggtaact aagtgctata acaagatata ttggtggagt tagtatggca
    25201 actattacta atgctcttaa tattgcggct actgttgctg aagtcttctc attaggagga
    25261 gccattgcat atggtttaga tatcgtagac ggtaaatttg atgggtattt atgggcgtaa
    25321 aactatagct atgaaacatt ttttttgcag gaattggtga aattcatttt ttgttatttt
    25381 tattatatct aagcatgtct gctagtccaa ttattggact agtgattgga tatactaaaa
    25441 taaatctaat aaattctatt attatagctc ttggtatgat attggcatta attttggtat
    25501 tatcaagagt gtatagaaaa tatttttata gagacgatga tgactaaagt ttttatcaat
    25561 aatttgggct ccttgtcaac tgtagtgggt tgaataaaag ctaacgtctg gggaagggtt
    25621 taaagctttg ctttatcaaa tattgactta cagagttgct ctattgtatt gatgtattct
    25681 tagtaaataa tatggtgttt taaatgaaag atgatcagaa atatttatta gcgggacttt
    25741 attcgcttct agttgcaata ttttattttc cattgattga gtccaagggg atttttgtaa
    25801 gtatattgat ggcggtttta cttttatatt taatatactt cattgcaaca gtcattcata
    25861 ttgtgataat taagtttata agaaaaaaat cttttaagta tttggttcta tatccattta
    25921 cttacgatgg gagctggaga tttcaaccta taaacttact ctactttcca gaaatggtga
    25981 gagatgtcat tccgattaat ttagttcaag aatattgcca aggacaacct tacggactgt
    26041 taaaaaagat gctgaaaaga attcgattgt cacgagagat atctttactc ctggctacca
    26101 ttatcgtcta tttttttacg catagaattc tacctctttc agtatttacg ttcatatttt
    26161 cttacatatt gctatttgca caatcttatt tgggtggtaa taccgcttgg attggaaata
    26221 gaagattaat aatagatgat gaatttgaaa aaatcttgct ttcaaaaagt tatataaaag
    26281 aaatatcatc tgcacgatac tcagaatact tgacatgtga atacaaaaac tttacaccaa
    26341 tcatattgtt agctattttt gaaaatttgc ttgactccta cttgcttcag aatcagagtg
    26401 aggttgactt agatattttt tataaagttt tacctttgct ttataaagaa aaatatacta
    26461 tgggattcaa ttactttgta agtttaaatt atttactata taaggttgga tttttgggaa
    26521 tcatttacga taatgaagca ttaagagatt tgtcaaaaca atatttaaac aaaaatattt
    26581 cggaattaca agatggttct ttcgagggcg gtatacaaga tgctgttgcc tctaaacaaa
    26641 tagtagttat aaatgaattt atagcttgtt taaattccag atgcatgcca tcccaatatg
    26701 atagattttt ttataaagat agaccttata ttttttccag aaagagtcct attaaggggt
    26761 gaaaatttgt tacatttgga ttaacaagtg tcctagggat tagaaataac tctagtatag
    26821 ctagatattt ttgagtaact agaaatatca ttaggctact catatgattg ccagaaaaga
    26881 tggctgatga atcttattta tactgttgca gtaagcattg tctaggataa ataagctatc
    26941 ttgatggggg agattggaat aattttagta gaaacattaa aggattatgg gcaagtagcg
    27001 caggattagg tgatgcaatg tgctaagttg gcttggtgtt agcagtagta ttatttaaca
    27061 atggaaagta gactaaatga aaaataaacg ttattttttt gacacaattt taatgatttt
    27121 actgcttata agcacgatat tttgtgtcag cccagtattt ataaaattgg acatcttagg
    27181 aactccctct catgccatct taacctttgt attggcgata ccgttattct atattttgtc
    27241 tcaatgctta catactttgc tattactagt ttcctcaatc ttttgtaaat taagaccaat
    27301 ttatttttat tttatatttg tgatcattat aggagctagg aaatactatc gaattctctt
    27361 tcaccaacta atgggattta gtccaggagt agcagtgttt tataaggaga gtcaaacaac
    27421 taagaatctg tttaaatttt actacttctt gtactttaca acactaattt catactattt
    27481 tttctttact tttgtgtatg acaaaccgtt gcttttgcct cttattccat tttctattat
    27541 catagcatta gtacaaaaac tttatagaat tgagaatcag caattatttc tgttaaaatc
    27601 aaaggtacta acaatactgg aatctaagaa gaattgtgag tttaatcttc aggattatca
    27661 tgaaatttgg aaattgcaat caaaatctga gttgccatgc gtggctttat cttatatcag
    27721 tttaatcaag ccatatcttt cagagagtgt gcgtgaacaa attgatttat tagaagttaa
    27781 gcgatttaaa aaaataaatc atccaatatc actttatggt atgttggatg ttataaaatt
    27841 aaatctatat ttacggcatt ataatgaaaa aaataaatat gaatcaatgt taaagaaaat
    27901 tttagaggta agaccagact ttgttttaat cgagcagaat attgatgata gtttaaatag
    27961 ctcgcaacca ttatctttat ctttagccat ttcagaaatc caacttttgc ttgaagtgta
    28021 tatggggatc aaacatgttt caattaggag ataaaaccag aataatattt gccattatag
    28081 gggcaagttg catattagtc agtatgtatc ttattccact tattaaaact gagaataaat
    28141 tttgggaaat acttttattt atattaggtg tattgctaat tggaattgcg ttactaagtc
    28201 aaaaaaaata aattttgaaa tagtattggt gagttcgcaa tcatttattg ctagtggttt
    28261 agaactgtta agtttcaaac aataattgat tttaaacctt aggtaaccta taaaaacaaa
    28321 taaggtagag aaaggatatc ttatctaggt agctaagtga taaaaacaca gtcgtctatc
    28381 acttgttagg aaactatcct aactatgctt aataaaaaag gagaaaataa atggaactcg
    28441 tattaccaaa taattatgtt gctcttgagc aagaagagat gatgtatctt gatgggggat
    28501 tttctattct gagatggcct gttgcaacag ccattaatat agcttttaat ggtgttttag
    28561 gtggaggagc aatcagtcta gttagaaatt atattcgtaa ttatggtttg cggcgagtta
    28621 caagcgcaat tgctggagca gctgcaagat atgttggggt acgagttgca aatagagtgg
    28681 caggatttgc actgtctgct attaatggat ttgcagcttg gatgtcaatt ggcgatgcta
    28741 ttacaacaat ctgggccaac aatgatgtaa ataggagaga cccaaattta aacgccttgt
    28801 ggtaactaga tatgggtatt aaaatggaaa aaatttttgt tattattttt tttgtatgtc
    28861 tatttatatc atccataact tttttagcct atgattttgt tagcgaagaa ataaaaaaat
    28921 tgattatttg gatgaatgtc gtttttttga ttttaatcat agcaatgata atttatccaa
    28981 aattaagaaa atgataagga cactgtaaaa ttatgatacg taaactaatc atttttttat
    29041 taatgctccc tctttttgtc ttgtggctag agttgcattt gttagtgagt aacttgcaat
    29101 tgaatctaga aattcccctt gattttgtgg ttagtacgag tcttgctttc tgtagtttga
    29161 ttttatcaaa aatagtttta gacatactat acgcattgaa agatttgtac aagaaagaag
    29221 ctcttattac gatttttcct tttattttta taggcaggaa gaaagtaaat gtgaggttct
    29281 cgccctattt ttcattccat cgtaaaagtt tgtcgcctga tgatttaaga tctaggatta
    29341 tatggagttt tattcttgaa attgctatta ttctagtatt tattttaaaa attccttttg
    29401 caatcatcat gcttactaca atatttttct ggactataat ggatatcaat catctcgttt
    29461 ttaataagac ggaatttctt tttaatcaaa ataaatggga aaaagaagat tcgtttgaaa
    29521 gcgatttgac gaaaacgtta aaggacaaaa tacaaaaatc agagttaagc tattctgact
    29581 taatgtccct tcttctctat gatgcgatga accaatccac ctttttaacg gatagtgaac
    29641 tttttgagga tattttgaaa aaaatcgaag attctcataa cacccttctg tgtacaggtc
    29701 ttgtcgagtt actgctgtat gagatttcta ttagtaacaa taacaactgg tccaacaagg
    29761 ttgataaaat tagaatacag ctcatcagaa tcaatcaatt agattttttc tattatacaa
    29821 gttggttaag gcaaaatttt gatttctgta tgaatagaga gtatcataaa atgaaatctc
    29881 ggaaattatt actgagtaat aaaaagatag tttaagttcg tcctatttgt aaaacaaatg
    29941 aaacaatttc aactaaggag gagaaaacaa atggaacttg tattaccaaa taattatgtt
    30001 gtgattgatg aagaagtaat gatgtatctt gatgggggag cttatttaag caagcgtgct
    30061 tgtcaaggaa tttgcgcagc agcaggtgta ttggctacag cagcagggaa aatagcttac
    30121 tgtattggat atggtgttct taatagaggt tgtgatatta acgggaaccc ttatccttgg
    30181 gatggattca tatctgcaac agtaagatga gtaatgtaga taagattcga aaaatacaca
    30241 tcatcgtgtg ttgggtgtat atatttttat cattcagggc gattataaat gatacggagt
    30301 attttttgct tattttttta gcttttatct actcaatagt atcactacct ctatattctg
    30361 tgaaaaataa aatagtatct atctgtctag ccataaattc gattctgtta atcagtttcc
    30421 ccattttaat caataagttt ttcccagaaa gttttttgac ttataccgta ttaataagtg
    30481 tttttatcct agagttagca atctttcatt taattgggga agattttgct actaaattga
    30541 ctaacgaata taagaaaatt agtcagttta gaagcaaggt gtctcaatct ccttggataa
    30601 aatatttaga gatttctagt tttatattaa ctatatttcc atctattctt tacggtacag
    30661 ttgataatcg tgtattggct cttatctttt tgataaaaat ttgtgtagat actacgataa
    30721 aatttttatt catcagatta tttgacacaa gtactttaat gaagaggaga atattttttc
    30781 ttttcgcatt ggatgttata gcttacttat ttttaggata tcttttagtg attcaaaaag
    30841 caggctattt gttttcggtt ttacttcttt tttctaattt ttcagttcca tttatcaaag
    30901 caaaggaata cgaattattt aaaaatagca agtaatagaa acataggtta aatcttagaa
    30961 cggcttaaaa tcagatatga taaagggaaa tagtcttatc taggtaacta agtgctacaa
    31021 acacagtcga ggatcacttg ttaggattac attttaatca tgcttaataa gaatggagaa
    31081 ttcctatggg gattgtatta ctctgaagtt tcgaatacct tagttctcgt gattttgagc
    31141 tgttctagct ttagaatcct tcagaatgta aaattttaag cagtcaaaga cttagaatag
    31201 cctggaaaca tttattttat agcaattcta ggcttagaat tgctcatata tatgtatttg
    31261 agctgctcga tagttaggac agctctattc tgagaaatct gagctatcaa tgcttcagaa
    31321 tagtatttcg caaatggttt ttattgaaga ggtgattgtt ttatgatagt ctaaaataaa
    31381 actgatttaa tggagtaaat ggagaatcgt cacatgaaac atttttttgc aggaattggt
    31441 gagataaaac ttgttggcta cttattgtat atatgtgtag gactagcccc tctttttcat
    31501 atatatgtca taggttcgga actgagtttt gtgaaaatag tgttatctat tttgggagtt
    31561 atatttgtaa gtatgttaac tattgctcga atttatcgga cttttctata aagaatagtt
    31621 tggacttgaa ctgtatctaa aaacagaggt tggtcgatta tcagcctctt tcttgtcgtt
    31681 gaaaagagtg agatatattg acttttgaaa aaaatgtcat aattgagttg ggataaacct
    31741 aaaagtaata tagtagattg aatctggaat agtccgttat gactgttaaa atatttctag
    31801 aaattaattt gactttccta atcgatttgt tcacatcttg tttcaatcta ctatactttc
    31861 tcgttaaacc gttcggcaat tcatggtgac atggacacat agtagtaagc acactatgtg
    31921 gcttggcaga gctaaaaact gttcccttgc gataagccta ataagcacaa catagggagt
    31981 tagagaagcc gactctaatc aaccactttg ggcagtagtg agaactgccc cgtgcttttt
    32041 tattttgaga aaatatggag tttgtcgttg aaattacttg attgtatttt ggactatcaa
    32101 gaaagattca atggaaaaac atgtcaagta tcaacgaatt ataagtattt agagattttc
    32161 aaagtgaatt tttgcttgac tgatttacat catttatttg acttatacaa aatcacacga
    32221 gattatgcta gtcaaacaaa acctgctatt caagctggtg tttttatttt agaagatttt
    32281 agaaatatcc tatgtacaat gatgtaatcg aaaggatatc tttatatgag tttatcggtg
    32341 atattttcta ttctaaaata ataagttgtt gcatcgtagc tagggattta tctaaaaata
    32401 ctatgaaatt ggacgtcata ttttttgagg ataaaaataa aagatccgca gttttaggtt
    32461 tacgaagaga caaaagcgga gtatttaaat cagttactct acattttaca agcgctaaga
    32521 aatatgctaa agttcgtaaa acagatgtga aagaaatgaa atggctataa agattgtccg
    32581 caggcttaag cttgcgtttt ttgttgttta acgaaattaa aaaacaagct ctctcatcgg
    32641 aaatgttttt ataggaatga aggaagatga tgctatgttt aaaaagagaa tcgaaaaggg
    32701 caaaagtagt gtctttatct ttttggagta gaagcttatg atatagaaat ttgtaataaa
    32761 aatgtaatat aaaacatttg acaaatattt acggggggag tatacttaat ataagtatag
    32821 tctaaaaatg actatcagaa aagaggtaaa tttagatgaa taagaaaaaa atgattttaa
    32881 caagtctagc cagcgtcgct atcttagggg ctggttttgt tacgtctcag cctacttttg
    32941 taagagcaga agaagctcca caagttgtcg aaaaatcttc attagagaag aaatatgagg
    33001 aagcaaaagc aaaagctgat actgccaaga aagattacga aacggctaaa aagaaagcag
    33061 aagacgctca gaagaaatat gatgaggatc agaagaaaac tgaggataag gcaaaagcgg
    33121 ttaagaaagt tgatgaagaa cttcaaaaag caaatttaga acttcaaaaa gctcatgtaa
    33181 aatatcaaaa agcacaaaga gaacttagtg aaagtccaga cagaagaaaa tctgctgcta
    33241 gaaagaaatt agctgatgca ttatcttata ttggagaggt agagttaaaa caaaaagagg
    33301 cggaagcgaa ttttaatacg gaacaagcaa aagtaattcc caaagaaact gagttagctg
    33361 ttactaaaca aaaagcggaa gaagctaaaa aagaagcaga agtagctaag aaaaaatttg
    33421 ataaggcagc tcaagaggta gaagtagcga agaaagaagt agaggctaag gaacttgaaa
    33481 ttgaaaaact tcaagatgaa atttctactt tggaacaaga agttgctact gctcaacatc
    33541 aagtagacaa tttgaaaaaa cttcttgctg gtgtggatcc tgatgataca gaagctatag
    33601 aagctaaatt aaaaaaaggg gaagctgagc taaacgctaa acaagctgag ttagcaaaaa
    33661 aacaaacaga acttgaaaaa cttcttgaca gccttgatcc tgaaggtaag actcaggatg
    33721 aattagataa agaagctgct gaagctgagt tggataaaaa agctgatgaa cttcaaaata
    33781 aagttgctga tttagaaaaa gaaattagta accttgaaat attacttgga ggggctgatc
    33841 ctgaagatga tactgctgct cttcaaaata aattagctac taaaaaagct gaatttgaaa
    33901 aaactcaaaa agaattagat gcagctctta atgagttagg ccctgatgga gatgaagaag
    33961 aaactccagc gcc
//
LOCUS       DBEROU010000022        33757 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.022, whole genome shotgun sequence.
ACCESSION   DBEROU010000022 DBEROU010000000
VERSION     DBEROU010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.022"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(229..591)
                     /locus_tag="ACL8CL_001112"
     CDS             complement(229..591)
                     /locus_tag="ACL8CL_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HGS1330366.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDAMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
     gene            605..796
                     /locus_tag="ACL8CL_001113"
     CDS             605..796
                     /locus_tag="ACL8CL_001113"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330367.1"
                     /translation="MKTKEQTRKLAAGCSKHCFEVMDRTDVVSSKHCFEVVDRTDEVS
                     NHTHGKATLTWFEEIFKEY"
     gene            complement(906..1064)
                     /locus_tag="ACL8CL_001114"
                     /pseudo
     CDS             complement(906..1064)
                     /locus_tag="ACL8CL_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(1337..2026)
                     /gene="rplA"
                     /locus_tag="ACL8CL_001115"
     CDS             complement(1337..2026)
                     /gene="rplA"
                     /locus_tag="ACL8CL_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HGS1330368.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            complement(2234..2659)
                     /gene="rplK"
                     /locus_tag="ACL8CL_001116"
     CDS             complement(2234..2659)
                     /gene="rplK"
                     /locus_tag="ACL8CL_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HGS1330369.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            2791..2928
                     /locus_tag="ACL8CL_001117"
     CDS             2791..2928
                     /locus_tag="ACL8CL_001117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330370.1"
                     /translation="MKIKEQTRKLAAGCSKYSFEVADKTDEVSSKHCFEVVNRTDAVS
                     R"
     gene            complement(2968..3684)
                     /gene="ldcB"
                     /locus_tag="ACL8CL_001118"
     CDS             complement(2968..3684)
                     /gene="ldcB"
                     /locus_tag="ACL8CL_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase LdcB/DacB"
                     /protein_id="HGS1330371.1"
                     /translation="MKKRYLVLTALLALSLAACSQEKAKNEDGTAKTEQTAKADGTVG
                     SKSQGAAQKKAEVVNKGDYYSIQGKYDEIIVANKHYPLSKDYNPGENPTAKAELVKLI
                     KAMQEAGFPISDHYSGFRSYETQTKLYQDYVNQDGKAAADRYSARPGYSEHQTGLAFD
                     VIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            complement(3687..4157)
                     /locus_tag="ACL8CL_001119"
     CDS             complement(3687..4157)
                     /locus_tag="ACL8CL_001119"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS1330372.1"
                     /translation="MCLICQRIELIKKGENPYFVKELETGYLVIGDHQYFAGYSLFLA
                     KEHVTELHHLKKETRLRFLEEMSLVQEAVAKAFAAEKMNIELLGNGDAHLHWHLFPRR
                     RGDMNGHGLKGCGPVWWVPFEEMTAETCQVKPEEIKRLVECLSSEVDKLLEIKE"
     gene            complement(4159..5196)
                     /locus_tag="ACL8CL_001120"
     CDS             complement(4159..5196)
                     /locus_tag="ACL8CL_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HGS1330373.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREISNYLVKTLEGFGYQPVRTAK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKLDRIGGFPWNMIEGENCTV
                     HVASTGEKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVTNEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYMEEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDDQQTDEYTVSICVKDASGPYHYDFRQHLVTLA
                     KNQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLRSNLVD"
     gene            complement(5294..6619)
                     /gene="brnQ"
                     /locus_tag="ACL8CL_001121"
     CDS             complement(5294..6619)
                     /gene="brnQ"
                     /locus_tag="ACL8CL_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HGS1330374.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            complement(6790..7077)
                     /locus_tag="ACL8CL_001122"
                     /pseudo
     CDS             complement(6790..7077)
                     /locus_tag="ACL8CL_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
     gene            complement(7237..7815)
                     /locus_tag="ACL8CL_001123"
     CDS             complement(7237..7815)
                     /locus_tag="ACL8CL_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HGS1330375.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            complement(7869..9269)
                     /gene="pepV"
                     /locus_tag="ACL8CL_001124"
     CDS             complement(7869..9269)
                     /gene="pepV"
                     /locus_tag="ACL8CL_001124"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HGS1330376.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGAARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVASVSLSEHGHTPHYVPMEDPLV
                     QTLLNVYEKQTGLQGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            complement(9282..9887)
                     /locus_tag="ACL8CL_001125"
     CDS             complement(9282..9887)
                     /locus_tag="ACL8CL_001125"
                     /EC_number="1.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HGS1330377.1"
                     /translation="MKFLELNKKRHATKHFTDKLVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            10058..10852
                     /locus_tag="ACL8CL_001126"
     CDS             10058..10852
                     /locus_tag="ACL8CL_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1330378.1"
                     /translation="MKKFSLLLAILPFLVACENQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIIFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQLQ"
     gene            complement(11034..11867)
                     /locus_tag="ACL8CL_001127"
     CDS             complement(11034..11867)
                     /locus_tag="ACL8CL_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS1330379.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQFLMERMDPA
                     TTVWLRSLPLLEKKEIAGLRFSISHNLPNKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELVNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRRDDNYQGHNLELLASLIEKHGYVEDVKNFFDF
                     L"
     gene            complement(11857..13701)
                     /gene="uvrC"
                     /locus_tag="ACL8CL_001128"
     CDS             complement(11857..13701)
                     /gene="uvrC"
                     /locus_tag="ACL8CL_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HGS1330380.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHDTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDETYFKSMAQEVSDFLKGQDNKIIDELKGKMAAAAQTMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVLNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPLEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            complement(13741..14442)
                     /locus_tag="ACL8CL_001129"
     CDS             complement(13741..14442)
                     /locus_tag="ACL8CL_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M57 family metalloprotease"
                     /protein_id="HGS1330381.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWAQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            complement(14521..15753)
                     /gene="murN"
                     /locus_tag="ACL8CL_001130"
     CDS             complement(14521..15753)
                     /gene="murN"
                     /locus_tag="ACL8CL_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HGS1330382.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSEYMSKLQGEQSKLEEKLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            complement(15758..16978)
                     /gene="murM"
                     /locus_tag="ACL8CL_001131"
     CDS             complement(15758..16978)
                     /gene="murM"
                     /locus_tag="ACL8CL_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HGS1330383.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMIWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            complement(17090..17869)
                     /locus_tag="ACL8CL_001132"
     CDS             complement(17090..17869)
                     /locus_tag="ACL8CL_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS1330384.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALTTNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            complement(17938..19599)
                     /locus_tag="ACL8CL_001133"
     CDS             complement(17938..19599)
                     /locus_tag="ACL8CL_001133"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGS1330385.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            complement(19774..21996)
                     /gene="recJ"
                     /locus_tag="ACL8CL_001134"
     CDS             complement(19774..21996)
                     /gene="recJ"
                     /locus_tag="ACL8CL_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HGS1330386.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            22273..23031
                     /locus_tag="ACL8CL_001135"
     CDS             22273..23031
                     /locus_tag="ACL8CL_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1330387.1"
                     /translation="MALVEFKNVEKYYGDYHAFRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKAVLENVT
                     LAPIEVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            23071..23835
                     /locus_tag="ACL8CL_001136"
     CDS             23071..23835
                     /locus_tag="ACL8CL_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HGS1330388.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYGIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            23848..24525
                     /locus_tag="ACL8CL_001137"
     CDS             23848..24525
                     /locus_tag="ACL8CL_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1330389.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLIILPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            24535..25194
                     /locus_tag="ACL8CL_001138"
     CDS             24535..25194
                     /locus_tag="ACL8CL_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1330390.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVT
                     RNGKNPVLRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            25452..26642
                     /locus_tag="ACL8CL_001139"
     CDS             25452..26642
                     /locus_tag="ACL8CL_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HGS1330391.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKTSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            complement(26963..27844)
                     /locus_tag="ACL8CL_001140"
     CDS             complement(26963..27844)
                     /locus_tag="ACL8CL_001140"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011184946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HGS1330392.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFFADGVKAI
                     QASVEERIDVFGSEGKA"
     gene            complement(27985..29313)
                     /gene="vncS"
                     /locus_tag="ACL8CL_001141"
     CDS             complement(27985..29313)
                     /gene="vncS"
                     /locus_tag="ACL8CL_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HGS1330393.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENSLTFFIDFPKVVQD"
     gene            complement(29310..29966)
                     /gene="vncR"
                     /locus_tag="ACL8CL_001142"
     CDS             complement(29310..29966)
                     /gene="vncR"
                     /locus_tag="ACL8CL_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HGS1330394.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            complement(30015..30098)
                     /gene="pep27"
                     /locus_tag="ACL8CL_001143"
     CDS             complement(30015..30098)
                     /gene="pep27"
                     /locus_tag="ACL8CL_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198524447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HGS1330395.1"
                     /translation="MRKEFHNVLSSGQLLADKRPARDYNRK"
     gene            complement(30278..31657)
                     /gene="vex3"
                     /locus_tag="ACL8CL_001144"
     CDS             complement(30278..31657)
                     /gene="vex3"
                     /locus_tag="ACL8CL_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HGS1330396.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            complement(31709..32356)
                     /gene="vex2"
                     /locus_tag="ACL8CL_001145"
     CDS             complement(31709..32356)
                     /gene="vex2"
                     /locus_tag="ACL8CL_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HGS1330397.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIR
                     LVNKKASKNTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            complement(32369..33646)
                     /locus_tag="ACL8CL_001146"
     CDS             complement(32369..33646)
                     /locus_tag="ACL8CL_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1330398.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
BASE COUNT         9595 a         7726 c         6281 g        10155 t
ORIGIN      
        1 tatacataaa ctaacctcct cattattagc aaggattcta ctgattataa cctaattctt
       61 agactacttc aagactaact gtccaataat aatctacaga taaatcatcc actcttaata
      121 aaattttatt caatccatct actcattccc aaaaggttgc tgtataatgc aagacatttt
      181 tcttactgat tttacttttc cttaccctca atttagtgag ctaataaatt attcttcttc
      241 aaaaggttga gatttatcta gtaactctaa aacattaatg gtaggtagca acattgcttc
      301 actgctatca attgccgaaa ctattgaaac aatagaacgt aaataatgat aaagaatagc
      361 agtaccattt acgtatagaa tatcggtgat attttcatct atatttattt caaaatatcc
      421 tattacagtt acagatattt ttttcatggc atctttgtca actaaagaaa cactaagttt
      481 tacggctcca tgactataat catctgtatg accaacctta actatgggat ataatatatc
      541 gaaggtaaat gtgaacaaac tgattaggga atttaaacca acttctaaca atgtgttata
      601 ctcaatgaaa accaaagagc agactaggaa actagccgca ggttgctcaa aacactgttt
      661 tgaagttatg gatagaactg acgtagtcag ctcaaaacac tgttttgagg ttgtggatag
      721 aactgacgaa gtcagtaacc atacacacgg caaggcgacg ttgacgtggt ttgaagagat
      781 tttcaaagag tattagaaag ctcgatggac tattccagat tcattccact agatttcgaa
      841 atcgagtagg ggataatctc tagcccctct cacaccaccg tacgtgccgt tcggcatacg
      901 gcggttcaac taacttttaa cgcatgtcgt tcaaggtaat aatccaaaca cgaaaccagt
      961 ccacgttttt caaggactgg ttttgatata gcacgtttaa gtaccgactt ctgagctact
     1021 aattgatagt agtcgcccca gccagatacc ttatccgcta tataggcctt attatcacgt
     1081 tcctgttcgt agagccacga tttcgctatc ccttcctctg acgattctta tttccagcgt
     1141 cagacttaaa acggtctatc cccagaccgt tttaatccgc tacctcacga tagtcagact
     1201 tgggaatcgc tattgggttc accggtagta ggtgcccacc gaggacttac acctcagata
     1261 tacgacatgc ccgtcgtaca tcaaaaaaag ttgagacata gcgtcttcaa cttttttaca
     1321 ttactttaac tactgattaa agtgagttta cgtcaacttt gataccaaca ccttgagtag
     1381 ttgtgatagt caagtttgtt acgtaagttc ctttagctgt agctggtttt gctttttgga
     1441 ttgtttcgtt gaaagcttta aagttttcaa ccaatttttc agcttcaaat gatactttac
     1501 cgatgattgc ttgaacatta cctgcacggt cagcacggta agtgatttta ccacctttag
     1561 actcttcaac tgctttagca acatccattg ttacagtacc agttttaggg tttggcatca
     1621 agttacgtgg tccaaggaca cgtccaagac gtccaacaag agccatcata tcaggtgtag
     1681 cgataactac gtcgaagtcc aaccaaccgt cgttgatttt agcaacaagg tcatcttcac
     1741 caacaaagtc tgcaccagca gcttttgctt cttcagcttt tgcaccacgt gcgaaaacaa
     1801 gaacacgtga agttttacca gtaccgtttg gcaataccat tgctccacgg atttgttggt
     1861 cagctttttt aacgtcgatg ttcaagttgt aagcaacttc tacagttgcg tcaaattttg
     1921 caaagttagt ttcttttgca agtgctacag cttcttctac gctgtatgct tttgtgctgt
     1981 cgattttctc aagagcagca cgaagttgtt tgcttttttt agccattttc tattctcctt
     2041 gtaagtggtt caatcgattt tcatctccca cgtcacttct cgaaatgatg aagtcttgcg
     2101 ggttttcagt ctactatttt tctcttccta cggccttaag ctcccttgct gtacccaagt
     2161 acaaggctca gtcacttgcc tagtatgaaa agcaactaat agactgctgt gagattttct
     2221 actgtgttat tgattagtca acaacagtga atcccataga acgagcagta ccttcgatca
     2281 tacgcattgc agactctacg tttgctgcgt tcaaatctgg catcttagtt tctgcaattt
     2341 cttgtacttg tgcacgagta actgtagcaa ctttagtttt attaggtgta cctgatcctt
     2401 tttcaacacc tgcagctttt ttcaaaagaa cagcagctgg tggtgttttc gtaacgaaag
     2461 taaatgattt atcttcgtaa actgagataa caactggaat gatcatacca gcttggtcag
     2521 ctgtacgagc gttgaactct tttgtgaatc ccatgatgtt gataccagct tgaccaagag
     2581 caggtccaac cggtggagct ggtgtagctt taccagcagg gatttgcaat tttacaagtt
     2641 tttcgacttt tttagccatt tttaaatcct cctttgtggt tttggcggta attagagatt
     2701 tttacctccc acaagtatgt ttttcacata cccatccatt atacactatt tatctggaat
     2761 tgcaagagaa aagtttattt tttgtactca atgaaaatca aagagcaaac taggaagcta
     2821 gccgcaggct gctcaaagta cagctttgag gttgcagata aaactgacga agtcagctca
     2881 aaacactgtt ttgaggttgt gaatagaacc gacgcagtca gtaggtaagg cgacgctgac
     2941 gtggtttgaa gagattttcg aagagtatta atcgacgtag tctccgcctt caaagccata
     3001 gtattcttcc aaactgagac cacttgcagc aatttcttta gcttcttttc ctacataacg
     3061 caggtgccat tcttcagcca tatagcctgt ttccttttcc ttgcctttga gataacggac
     3121 aacaaagcca taatcagctg catgatccaa gagccattgg gctgcttttt cttctgtcac
     3181 caaatcacca tcagtcccaa tcacatcaaa ggccaagcct gtctggtgtt cgctatagcc
     3241 aggacgggca gagtaacggt cagctgctgc ctttccatct tggttgacat aatcttgata
     3301 gagcttggtc tgagtttcat aacttctaaa accactgtaa tgatcactaa tagggaaacc
     3361 tgcctcttgc atcgctttga tgagtttgac caactcagcc ttggctgttg gattttcccc
     3421 gggattatag tctttcgaca aaggataatg tttattggct acgatgattt cgtcgtattt
     3481 tccttggatg ctgtagtaat cacctttatt gaccacttct gcttttttct gggcagcccc
     3541 ttgagactta ctgccgactg tcccatcagc tttggctgtc tgttctgtct tagctgtgcc
     3601 atcttcattt tttgcttttt cttgtgaaca agctgctaga ctcaaggcta gcaaggctgt
     3661 caagacaagg tatctttttt tcatttctac tcctttattt ctaatagttt atctacttct
     3721 gacgataaac attcgactaa tcttttaatc tcttccggtt ttacttggca agtttctgct
     3781 gtcatttctt caaagggaac ccaccaaact ggcccacagc ccttgagacc gtggccattc
     3841 atatcacctc ttcgtctagg gaaaaggtgc cagtggagat gggcatcgcc atttcctagc
     3901 agttcgatat tcattttctc agcagcaaat gccttggcaa ctgcctcttg gactagactc
     3961 atttcttcga gaaaacggag tcttgtttcc tttttcaaat ggtgcaattc ggtaacgtgt
     4021 tccttagcta gaaaaagact atatcctgca aaatactggt ggtctccaat cacaagatag
     4081 cctgtttcca actctttgac aaagtaggga ttttctccct tcttgatgag ctcaattctc
     4141 tggcaaataa ggcacatatc aatctactaa attgcttcta agataggcat cgaccattcg
     4201 ttctgttgcg accaccgagt caatatgagt gcgctcataa gaatgactag actcgatacc
     4261 cgcaccaaga agagcgtgtt taacttctgc acctgcagac atagccgctg aagcgtccga
     4321 accataaaat ggatagatat ccagcttgaa tggaatatct tggtttttag ccaaggtcac
     4381 caaatgttga cggaagtcat agtgataagg acctgaagca tccttgacac agatggacac
     4441 tgtgtactca tctgtttgct gatcatcccc catagctccc atatccacag ccagatattc
     4501 gactacttga gcaggaatat tagaattagc accatgcccc acttcttcaa agactgaaaa
     4561 ggcaaaatgg gttgttactg gcaactcaat cttctcttcc atataaatac gaagcaggtt
     4621 aagcaaaatc gctgcgctga ccttgtcatc caaatgacga gacttgataa aacctgtctc
     4681 tgtcacgaca gttcgtggat caaaactgat aaaatcaccg acctcaatcc ccaaggcacg
     4741 agtttctttt tcattggtta ctttggcgtc caaacgcact tccatattgt cctgggtacg
     4801 ttctgcagtt cctgcatcct tgtagacatg gcaagaagtt tggtggatga ggatggtgcc
     4861 tgatactttt tcacctgtgc tagccacatg aacggtacag ttttcacctt caatcatgtt
     4921 ccaaggaaaa ccaccgatac ggtccaattt aagacgacca tctggtttga cagcacgaac
     4981 aatagcaccc agcgtatcca catgagcagt cacatagcga tgttgctcat cattttgacc
     5041 tttgatggtc acattgacac cacccttagc tgtgcgaact ggctggtaac caaaaccttc
     5101 tagagtcttg actaaataat tcgaaatttc acgagtaaag cccgttggcg atgcgatggc
     5161 tgtcagttct ttgatatatt ctactgtttg attcatttct tcacctcttt cgctacctat
     5221 tataacacat ttatcatcgg ataaaaaaag aaaatcactc ctgtagactt ggctacaaaa
     5281 gtgatttaag tgattattcc atttcaaaaa catcgctttc ttgcttgttt ggtagaacca
     5341 atgagagcaa gattccaaca atggctggca ccaaccatgg gagagatgcc ttggcaaaag
     5401 gaagaacgct aacaagattt gcaagaaact caaccttaaa cgagcttcct agtacgcttg
     5461 caatggcaat aactgtaacc acagcaattg tcaactgcat acctggtttt gaaagggcaa
     5521 caaatttgtt gacaatgaca atcataacga tagcaatcgt gattgggtac aagataacca
     5581 gtactggaat tgagtacttg ataatcgcat caagacccaa attggcaata gcaaatccaa
     5641 tcaaggtaaa ggctgtcgca taaaccttgt agctgatttg tgggaagcgc tcattaaaga
     5701 actcagctgt tgacacaatc aaaccaacag tcgttgtgaa gcaggttacg gtaaccatag
     5761 ctgcaaggaa gagttgagct gttgagccaa agatttcttg agtggcttgt gacaagatgt
     5821 aaacacctgg tgttccaccc ttcatcgctt cagctggtac tgggaaatga tttccaagaa
     5881 aacctaaacc gatgtaaaga gcgctgaagg caagggcaac aacgatacca acaacccaaa
     5941 tagttgaaat gtattctttc ttacttgaaa atccaagttg tttcaaggtt tgaactgcga
     6001 ttacgctaaa ggccactgag gcaagggcgt ccaaggtatt ataaccttct aggaaacctg
     6061 taccaaaggc agaagcttga taagcagctg aagcagcttg aggacttgtt ccaccatatt
     6121 tgatagctcc cagaacgacc aagataacaa tcaaaattgc aaagactggc gttaaaatac
     6181 gtccaatgcg gtctaagatt tttgatggat taagcgaaat caaataggct gccgcaaaat
     6241 acagaaccgt aaatacaatc aagccaagtc ctttatttgc atccgacaaa agggggctaa
     6301 tccctacttc gtaagctgtt gtagcagtac gtggggtagc aaagaatgga ccgattgaca
     6361 agtaaagaac tgagaggtaa agagtcgcaa accaaggcgc tatcttcgtt gaaatctcgt
     6421 agatatatcc tttaggattt agcgttccaa taataagggt caagacggcg ataccaacgc
     6481 ctgaaaagac aaaacctgcg atggcaggaa gaaaatgttc tccagataga gcacctagag
     6541 aaggcggaaa aatcaagttc cccgcaccaa aaaatattcc aaacaggagc aaacctgtta
     6601 gggtaccttt tttagccata aaaatctcct tcatatttat aaaatactga cctagtatac
     6661 cacgattagg agtctgaaaa aagcatcacg aggcaaatat taaatgtttt caattttctt
     6721 aaaaataaga attatctaaa aataaaactc tcctacccag tccacaccaa gtaggagaaa
     6781 tgcaaaattt taaagttctt caaaggctaa catgccaccc tggacattga taacattata
     6841 accttgttct aataggaatt ggcaagcacg cgccgatctc attccagatt tgcaaataat
     6901 ataatgcaag cgatctttgt ccaattaatc ataactatca gccaattgac taagcggtag
     6961 gttgtaggca ccttctaaat gaagagctgc aaactcatcc acttctctca catccactaa
     7021 agaaagctgg tcgttttggt aaagctggta aaatgcgtca aaggctattt ctttcattct
     7081 ttttcttcta atactcttcg aaaatctctt caaaccacgt cagcgtcgcc ttaccgtgcg
     7141 tatgttaccg acttcgtcag ttttatctgc aacttcaaag ctgtactttg agcagcctac
     7201 gactagcttc ctagtttgct ctttgatttt cattgactat aaaatggttt taattctttt
     7261 tttcaaatct ggcactactt ctgcctcaaa ccaaggattt ttggccatcc agatttgatt
     7321 tcgtggtgat gggtgaacta gcggaaaata ggctggcaga tagtctttat agtgtttcac
     7381 cctctccgtt accttcccac tgattttctc ctgtaaatag taggcttggg catattgccc
     7441 aatcaagagg gttaactgaa tatcaggcaa ttcctgtaag agctgcggat gccatttttc
     7501 tgcaaaacct gtacgaggcg gaagatcacc cgacttgcca tgtcctggaa agtagaaatc
     7561 cataggcaaa acagcaaaat aacctgaatt gtaaaaggta tcttcatcca cacctagcca
     7621 gtcccgcaag cggtcaccac ttttatcttt ccagtaaagg cctgcttctt gagttttaag
     7681 tcccggagcc tgaccgatga tattgatgcg agcagttttt ggcgctgcaa agagaggctc
     7741 aatgccacgc tctgtatagc tggcattctg cgaatccgcc atgatagcct gtttgattct
     7801 ttcgatttga gacatctact ttccctccaa aaagaagtcc aagcataaga tctcagactt
     7861 ctatcgtttt atttgatcaa ttcgtaaata gcttcggcat aaattgctgc tgctcggaag
     7921 agatcgtcca aggcaataaa ttcattggct tggtgcatgg tatcaattga gtctgggaac
     7981 atagcaccat aggcaactcc gcgctctaac aagcgaccaa aggttccacc accgatgact
     8041 tgttcatgac cttgtaggcc tgtttgtttt tcatagacat tcaacaaggt ttgcacaagt
     8101 ggatcttcca ttggcacata gtgaggggta tgaccatgtt ctgatagact gacagaagca
     8161 actggcaagt tttctaggat tgatttgatt tgttctggac ttgttccttt tggatagcgg
     8221 atgttaaggg caatggtatt atcagcactt gtttcatcga agcggaagac tccggcattc
     8281 atagaaagag cacccatctt ttcatccaca tgagcaatct tgagattttc accctcatga
     8341 tcgttcaaga gaattttacc tgcgatgtca aggtagtctt tggctggacc agcaaagcca
     8401 aactggctga ggaagagggc aaggtaagtt gcgccattga cacctgaagc aggcatagca
     8461 ccgtgggctg atttaccaat gatcgtcacc ttgtatttgc cagcttcttc ttggagttct
     8521 cctctaagtt tgtgttctgc aacaaaggca tctagtttag cttgcaagtc agccaaatca
     8581 cctgaaacga ctgctgttgc tgattctggt accatatttt cacgtaaacc acctgtaaag
     8641 ctgtgaagac gggcagcacc tgtattttct cctgcaaagt ggaggtattc cgtgatattt
     8701 cctttttcac cattgatgat tggaaattca gcatctggtg agaaaccgaa atctggtttg
     8761 gcaagtccta cgtgctcaaa gtagtagtcc atgtctgccc agcctgattc ttcgtctgtt
     8821 ccaacgatga agcgaacttt cttagaagtt ggaagaccca attctttgat gattttcaaa
     8881 ccatagtaac aagctgttgt aggaccctta tcgtccgaag ccccgcgcgc ataaaggcga
     8941 ccatctttga tagttggtgt gtaagggtct gtgtcccaac cgctaccagc aggcaccaca
     9001 tccatatggg caaagattcc gagaacttct tctccatcac caaactcaaa atgtcctgca
     9061 tagttatcaa cattcttagt tgggtagcca tcgcggtctg cgatttcaag gaatttctcc
     9121 aaggctttta ctggaccagg cccaaatgga tgctgggcat cagccttgct gtcatcacgt
     9181 tctgaattga tttccaaaag gctaaacaag tcagccaaga ggtcttcttt gcgtttttct
     9241 acttctgctg taaaatcaat tgctgtcatt ttttcttctt tctatctttt ctcgatgatt
     9301 tcatccactg gcaagcggta gcttggttcc aatttttcgt ctgtataacc cactgtgatc
     9361 aagagttctg ggcggaaacg gtcttcgatt tccaaaactt cattaacttt tgatttgtca
     9421 aaaccaagaa taatgttaga accaattcct tggtctgtca atgcaagaac caagttcatg
     9481 gcaaccaaac ctgcattgag agctaggtag tcgctgactt gttgctcact gtaacgggca
     9541 aactcagctg gcagattttt cataaaatat tgaagttgct cttcagaaaa gttattagca
     9601 ccaccaacac gggcaatctt acgagcacgt ttggctaagt ccgtatctgt aaacaaggca
     9661 atggttacag gcgctgatga tacctgttca aaattggaac cataagctaa ctttgccagt
     9721 tcagcatttt tctcacgtac caccacaaat ttccaaggct ggctgttgtg ggcgcttggc
     9781 gccaaggttg caatttcgat agccgtacgc acatctttgg gatcaacaag cttatcagta
     9841 aaatgcttag tcgcatgacg ttttttattt aattcaagaa atttcataat cgatttcctt
     9901 ttctaattct actgcttcca ttctaccata atttgaaaga gcttgcaggg atttttcatg
     9961 attaagtttt tctatcacag acataaaaga tatatattgg tttatcagga aagtttctga
    10021 taaactagca agtactttac atttgaatgg agacagtatg aaaaaattta gcctattact
    10081 agctatccta ccatttttgg ttgcctgtga gaatcaagct acacccaaag agactagcgc
    10141 tcaaaagaca atcgtccttg ctacagctgg cgacgtgcca ccatttgact acgaagacaa
    10201 gggcaatctg acaggctttg atatcgaagt tttaaaggca gtagatgaaa aactcagcga
    10261 ctacgagatt caattccaaa gaaccgcctg ggagagcatc ttcccaggac ttgattctgg
    10321 tcactatcag gctgcggcca ataacttgag ttacacaaaa gagcgtgctg aaaaatacct
    10381 ttactcgctt ccgatttcca acaatcccct cgtccttgtc agcaacaaga aaaatccttt
    10441 gacttctctt gaccagatcg ctggtaaaac aacacaagag gataccggaa cttctaacgc
    10501 tcaattcatc aataactgga atcagaaaca cactgataat cccgctacaa ttaatttttc
    10561 tggtgaggat attggtaaac gaatcctaga ccttgctaac ggagagtttg atttcctagt
    10621 ttttgacaag gtatccgttc aaaagattat caaggaccgt ggtttagacc tctcagtcgt
    10681 tgatttacct tctgcagata gccccagcaa ttatatcatt ttctcaagcg accaaaaaga
    10741 gtttaaagag caatttgata aagcgctcaa agaactctat caagacggaa cccttgaaaa
    10801 actcagcaat acctatctag gtggttctta cctcccagat caattacaat aacactcttc
    10861 gaaaatctct tcaaaccacg tcagctttat ctgcaacctc aaagctgtgc tttgagcaac
    10921 ctacggctag cttcctagtt tgctctttga ttttcattga gtataagata tactaaaagc
    10981 gattggacca gccaatcgct tttttagttt gcattggcta ttttaggaaa ctcttacaaa
    11041 aaatcaaaaa aattcttcac atcctctaca tacccatgct tttctattaa gctggctaaa
    11101 agttccagat tgtgcccctg atagttatcg tcacgacgca gttcttcata catttcaata
    11161 aagggaagcc ccttggactt ggccaattct aactcagcct cgtaatcata agcaacttta
    11221 cgaaattgga tattgaccaa ttccccatct tcaacttcta tcacggcata ctgggcacgg
    11281 tgatttttta acgcctccca attaaaatag ggcatgccaa tcgaccctgg attgatgatt
    11341 tgttgccctt gacttccata acgaagcaac tgcttgtgaa catgaccata aactgccacg
    11401 tccgtttccg catctagcag ttggtcaaat ttctctgtat cattctcaac tagcaagtca
    11461 ccaccatagt tcttatttgg caaattatga gagatagaaa agcgcagtcc agcaatttct
    11521 ttcttttcca gcaaaggcaa gcttcgtagc cagacagtcg ttgcaggatc cattcgctcc
    11581 atcaaaaact gggtcatacg caagagctga acttcctgtg ggtcttctaa gccatattgc
    11641 ccatctaaag cctcaaggac acgatcatcc caattgcctc gaacacttgc tgtgatagga
    11701 aggtccttta gcagggcgac taagtcattt gcgcctggac caggaagaaa aatatctccc
    11761 agaagccaat attcactggc cccttgattt ttagcatctg caatcactgc ttctagcgcc
    11821 gtcgcattgc catgaacatc tgataaaatt gcgattttat ggttcattgt ggtttccttc
    11881 cgtttggtaa tctactcttt cttctgctac ttgaggcaag gcttctccct gcgggttcaa
    11941 ctttctttgc acagcctctg caacgactct aggtacccca acttcgacaa tctcatccac
    12001 actggcttcc ttgattttgg tcaaagactt gaaatgcttc ataagattct gcttgcgttt
    12061 aggtcccaga ccgtcaatcc catccaattg agatgagaaa gaatttttgg agcgcagttg
    12121 gcggtggaag gtaatggcaa agcggtgcac ctcatcttgg atgcgttgga ggaggaaaaa
    12181 ttcctgagaa ttgcgagaca agtccaccac ctcaagcgga tctccaaaga gcaattcatg
    12241 ggtttggtgc ttatcattct tttgcagccc agcaattgga atatccaagc ccagttcctc
    12301 ttggataacc tgcttagcga tattgacttg accttgcccc ccatcaatca caatcaaatc
    12361 tggaggagtc aaagcctcac gctgtactcg accatagcgt ctgcgaatga cctctctcat
    12421 gctggcatag tcgtctggtc caacaaccgt ttttatcttg tacttacggt aatccttctt
    12481 actcggttta ccgttgacaa agaccaccat agccgaaaca gggctagttc ccatgatatt
    12541 agagttatcg aaggactcga tacgtactgg ggtcgggatt tggagcaaac gtcctagatt
    12601 ttcaatagct ccttgggtct tctcgacaga tttctctagc aggttaaatt tctgctccag
    12661 actaacacgt gcatttttta tggctagatt gaccagctgt tttttctctc cacgttgggg
    12721 tttgagaatc ttggtatcca ccaaagcctt gacagcctct tcgtcaatat cctgcggaat
    12781 cagtacctca ttgagaacta ggtgagattt ttcttgatag aattgtccta cataggtcaa
    12841 aaaatcctca tctggatcat tgaagtaggg gaagagattg acatcgcgct cgatgagctt
    12901 accctgacgg acaaagaaaa cctgcacaca catccagccc ttatccacat agtagccaaa
    12961 gacatcgcga ttttggagat ccttagccat gacccgttgc ttggttcgaa gcgttccaat
    13021 agcctgaatc aggtcacggt attccgccgc acgttcaaac tccatagtct gggctgccgc
    13081 tgccattttg ccctttagct catcaatgat tttgttatcc tgccctttca gaaaatcaga
    13141 aacctcctgg gccatagact tgaaataagt ctcatccttc ttacagatgg tgtgggccat
    13201 gcattgaccg atatggtaat aaaaacagac cttagagggc gggttggtac acttacgaaa
    13261 agggaatatc cgatccagca accgcttgat ttcattggct gcccccacat cgggataggg
    13321 tccaaaataa agacctccgt cctttttgac ctgacgagtg ataatcaagc gtggatagcg
    13381 ctcattggtg attttgatga aaggatagga cttgtcatcc ttgagcatga tattgtactt
    13441 gggcttgttt tccttgatca ggttgatttc taggagaagt gcctcaatat tagactccgt
    13501 aacaataaat tcaaaatcca caatttcaga caccagagcc tctgtcttgg tatcatgact
    13561 tccacgaaaa taggaccgta ctcgattacg cagattttta gcctttccta catagataat
    13621 ggtgccattt ttatccttat gaatgtagca accagggctg gtcggcaaga gctctagttt
    13681 tgatttaatc aagttgttca tagttccatt atagcaaaaa aagtagggaa agatagtacc
    13741 ctactcacta gtctcatata tttttcggag gtttgcaaca tcctcttcct ggataccata
    13801 aaaggaacct gctggttgca tgacagactt ctcatctgta tggtccaagc caatcgcatg
    13861 acctaactca tgttctgccg tatggacaag gcgttcatag gagtagccat agtatggatt
    13921 ggacaaataa taatgattca accgcaccgt tacggacaag aattgccctg ttaagagatt
    13981 agtttgactt tccgcctctc ctgccacagg agtgcctccg tcgttcatct ccgtagccgt
    14041 aatatccgcc ttactagact cagtcacgag ttcaaagtta aaagcacccg tttgattcca
    14101 gttctggatt gcttctaaat aagcctcttg aaagcgtgaa tccatctgcg gatccaggta
    14161 aatacgagca gaggcctgtg cccaccttcc ttcagaatgc tggtgactat ctgtctggct
    14221 taacttaggc agttgccctg ttttctccca ttggtcaata ctttgttgac caatttttac
    14281 tgaccgttct gcttgcttta gcgctccttg aaaatccccg ttcagatacc agagaagtcc
    14341 gaaagcaagc acacataaga gcacaactat ccaaaccaga cgccaaaaca aacgccacac
    14401 aaaagaaaag aaagccccta tcaaacgaaa aagccagcgc atgtctctcc acctttctag
    14461 caaataagaa ttattttact aaaagaagct aaaagaaatc taaatactga ctttttcatc
    14521 ttaacgtcct actatttttt tgagtaactg gatagctttg tattttaaag gcgatggatg
    14581 gtaacggaaa gtacccgctt tgcgtacaat atagccatta aaattctgtt taaaacgcaa
    14641 aacaccatca cttccatcaa aaatcccttg aatgcctagg aagttgtatt taggtattcc
    14701 acgttttatg ctttccaaca taacatattt ttgaagcagt gcaggggcat agaacttatt
    14761 aaactcagtg taggaaccac taaagagata agtcgtttcc tgaggcatat aaacaaataa
    14821 actaccagct aaaacaatat cttcttctcc atatttttca atcaagtctc gcgcttctgc
    14881 ttttcgaact tcaaacgttt caaattgact agaatattct ctcagttgat tttgtttttt
    14941 ctcagaatga ggatttttac tcaaatcaag tcgcaacttg tccaattttt cttctagttt
    15001 actttgttca ccttgcaatt tgctcatata ctccgaaaaa ttcaagcttg ctatgagaaa
    15061 ctccgcttgt tctccaaaag tatcataaaa atgctcataa tattctaaac ttttatcact
    15121 atattctcta cgttcagagg tttcttttgt tatattctta aaaatcgata gttcttcacg
    15181 ttttaacttt ttcaaccgaa tgccaaaggt ttcagccttt ttcaccaagg gtttaccctt
    15241 tttgctaaaa cttttaagca aactcttttc agttaattca gttaaatctt tatagtataa
    15301 ccaatctggt tctccacctg ggtaacctgt tgttaagcca tcaaattgat aacctaaatc
    15361 agtcaaatct tgaataatac tttttttctc agcatctatt ggattacctt ggctatcaaa
    15421 agtttgataa gtttcatagg gttttacaag caactctaat acaccatttt gcttggcata
    15481 ttcttttaac tctgcataaa aaactggaag agcatcttgt tgggtataaa tcggccccga
    15541 attgagttcc atatgcagac cacccagcat gggcaggcta taaaccagag ctgcaacttg
    15601 aatttctcct tcttgtttca aagcaagata aacaattcga gcccctcttt tttctagcaa
    15661 atcccccatc tggacagatt gcataaagga acgagaagaa acctgatcag aataagtctg
    15721 aaactcttct ttcgtgagtg ttgttagtgc catatactta ctttctatgt ttttttctta
    15781 atgttttacg gaaatcaaga gcaagtctta acagaggata gagaggatga gtgggcattg
    15841 taaattcacc caagtattct tcaatcgttg gattaaattt ttccttaaaa tgataaagtc
    15901 caccattgag agagttttca acaccaccta aattttgcca gatcatacct cgttcaaagg
    15961 catagcgagc cgtttcatac caagttaaaa ttggtgcatt gtaacgttta aaatcatcat
    16021 ccataccagc atatatattg acagaggtag taccaaattc caaactcaaa gtagccgcta
    16081 aaggaactct cgcttgacct acatctatat attcctgcaa gaaggtcaat tcctctaaca
    16141 aacgttcttt ttccttcttc tgcgcttcta cttttgaagt tcgagtcgac tcagtaaagg
    16201 tctcttccaa ggctctattt ttcgctaact gttcttctaa ctcttgcgaa cgtttagaaa
    16261 catccaaggt ggccaaggtg atataggcct tgtccttaaa attatctaac aattttttat
    16321 aataggcttc attcctcaaa tgaatctctt ttcgcttctc agtttttttc atcaactccg
    16381 aaaatgaatc taatagttcc agtccaccat attgaatctc aagccctttg tttcgtgctg
    16441 ttcgaatagc ctgttttgtt gacttggaaa gtttatcttc ttcaaaattt tccttgtata
    16501 ttttcgcctg aatacgaggt tgaatggtgt ctcccatttc ctccgttttt cctgaccacc
    16561 ttactcccat ttgttgcaaa ctatcaataa tagccagatt ttcaggaaat tctgtctttt
    16621 cctgattgat taaactttga gataggcaaa tacttgggtc aaaagtcaca aaaaccgctc
    16681 tcttactgcg agcataggac ttaatagact gaatggcaaa attcaagagt tctttatccc
    16741 cataatccaa tataggtcct cttgggatgt aaaacatttt atagcctagc ggaagagttc
    16801 taatcaaaat actagctgtc gccagtaatt tttcttccct gtaaacacca aacttctcat
    16861 gttgccaatt agacttaact tcctcccaag cactactttg taatacattg gctaattcat
    16921 gttctttgac aaactgatca tattctaatg tgggaatgcc aatttgataa cggtacattt
    16981 cctactctct ttcctccagt attttattac tattctacta ctttcgtttg aaaagtctag
    17041 tgaaaacata ttttatttta gttctactcc ccctatgctg aagttcaaac taagtcagtc
    17101 tctcttctaa tttgaaatca attggtaggg ttgttaaaaa aacttccaat tgtttttccc
    17161 agtagtacca ctcgtgagtt ccagcgctat ggctataggt cacatcaaaa cctagttttt
    17221 tgagattttt cactgcgaga ttattggctt cgtacaagaa atcctgttcg ccacaccacg
    17281 cccaaagttt ggtcttttta tccgattttt tagccagact ttcaagagaa tagggactag
    17341 ttgtccagtc tctaatctct ccaaaaacac ctctccagta ggctggactt cccagatttt
    17401 gactttcagg agaaaagttt tgaaagctga gggcacctga aaaactagct gcatgagaaa
    17461 aacgatttgt cgtaagagcc agtttgaagc agccgtagcc tcccatagaa agaccagcga
    17521 taaaggtctt ttcacgcttg ctcgtcatat tagggaagaa gcgtttcaga acctgtggca
    17581 attcctctgc tagagccgtg tagtagtcaa aaccatactg ggtatcggtg taccaaccat
    17641 tgctggtatt gggcataaca acgatgagat tagttcctcg aagcaagcgt tctacattgg
    17701 tccgcttaag ccaactatta tgatttccag acatcccgtg caaaaggtac aagacgggaa
    17761 tatcttcaca ctctggttct tccactcgat tggcatcagg gtagaggaca ttcacccccc
    17821 actccatatc caatacttgt gagtaatact cgattttcat tactgccatg attttctcct
    17881 tctatttttc tataagaaaa agccgaaact cgactttctc tctgtttatt tcaacgatta
    17941 ttttgcttcc atgactactg gtaaaatggc tgggcgacgc ttggtttgat caaagaggta
    18001 cttggtcaga ttgtcacgaa ccttaccttt gagatctgcc cagtcaaagt catctccttg
    18061 aagatactct tctaccgttt ggttaatcaa ttctgaactt tcacggagaa tatcgcgact
    18121 cttcttgaga taaacaaatc cacgcgtgtg aacacgagcc ctagccacaa ttttcttctc
    18181 acgacggttg actgtaatag ccacgatgaa aattccatcc tctgacaaga ccttacggtc
    18241 acgaagaaca acatttccaa catcaccaat ggcattccca tcaatcaaga tatctcctgc
    18301 tgaaaccgat ccagctggaa caaagtctcc attctcgtaa gccatggtcg tccccttttt
    18361 aggaatgaag atgcgttctg gcaacatccc aactgccatg gcagccttag cgtgagcatc
    18421 caactcacga tactcccctt ggacagggaa gaggtactta ggttgcaaaa gattgatcat
    18481 cagctgcaaa tcacgcacat ttccgtgccc tgatacatgt aaactttggg taatcaattt
    18541 gacaacccca cctgcctgat aaatcatatt ttccacacgc gcaacaaagg cttctttagc
    18601 aatagacgga gccgtagcaa tatagactag gtccccatcc ttgatttcta cataacgatg
    18661 gcgaccaatc gacatcttac gaagtccatt gataggctca cccatacgac ctgtctcaag
    18721 aataatcaac tcatggtctt caaagcgaga catatcttta ggcttaatca aaagaatttc
    18781 gttggctaaa gacaacttct taagacgaat cgctgtgcgg acgatatttt caatatcaaa
    18841 tcctgtcaag acgatacgtc gacctgtttt atccgcagcg tcaaaaatct gctgaatacg
    18901 agaaagatta ctggaaacag ctgcaacgat gatacgacct tcccagtcag caatagtttg
    18961 ggtaatttca tccctaactt cactttcact agccacctga atattgctgt ctgcattggc
    19021 cgaatcactg aggagagcca ggacgccgtc acgaccaatc tctgccaaac gagcaaaatc
    19081 agttgcataa gattcactag ccgtttggtc aaatttgaag tcacctgtat aaacgatgct
    19141 tccttccgat gtcttcaaga caattcccag actctctgga acggagtaag tcgtagggaa
    19201 gaaggaaacc actgtcccac caaaatcaat ctccgtattc tcatcaatga catggaaatc
    19261 attaaatttc ttaacggcat catttccttt gacaaagagc tttgccaact caatggtcaa
    19321 ctcagaccca aatacaggaa ctttagcctc tgccaagaga tacggtagag caccaatggc
    19381 atccgcatgc ccgtgggtca agaaaacccc agcaatacgg tcgctatttt caaaaaggta
    19441 atccatgttt ggaatcacca catcgacccc taattgttca ttttcaggat attttaaccc
    19501 tacattcaaa acaaaaatgg actctccaat ttcagcaatg tacatatttt ttccattctc
    19561 acgcacacca ccaagtgttg ttaaactgat attactcatt tttcctccaa aatcttagtt
    19621 tatcttgatt atagggcaat tctaccctct tattctaaaa gttctgatac tttcagccga
    19681 atcttttcct accattatac catttttttg atgattttca aaatgaaggt ctgttttcaa
    19741 aaaagagttg atttcccaac tctttcctaa cttctactct ttttccatca aaaaatcata
    19801 aatttcttgc accgtaccca gcgccatcat ttcttggtct ttaacggttt gtttgagatt
    19861 ttggtaaatt tgactttctc ctatctcccg ctttggcgcc tctttattga ctgtcatcac
    19921 accatctttt atcgtcacaa agcctagttc ttcaaatact tgaatcatct tgaccagcaa
    19981 gatttgttga atattaagat atgcagccaa atctttcagc ttgtagcgaa tatcaaactc
    20041 tgggaactgg taaatagtct tgtacaattt ggcaaactga tctctagtcc cataacctgt
    20101 cagataataa gccttgtcaa tatcattttt gaaatagaca gcagagaaat gctgttcctg
    20161 aaaaatggtc ttcagctgag taatatcctc tggaatgttt tttacgacaa cagcttcact
    20221 agccgcaaga tttggcagtt ctccaggaaa atccaagact ggaacacctt ctggcaagac
    20281 tgcatttttt ccacgaatgt taaaaagttg aacaccttcc actcgcgcat ccaccatcat
    20341 caactggagg gcagtttggc cattccattg gttgacagac aatttaaccg ctaactctag
    20401 attcttggtt tgagaaaact ctgtcgccca tctgccttga ccaaaggcta ccacttcaaa
    20461 actcgcctca cccttggaaa ttttcagctt tagatgggca ttacctgccc ccatagtacg
    20521 agcactttcg acctgaaaat tcttgatata aaaaataggt ttctgattat ccattccaaa
    20581 aggagctaaa cgttcaaaac ttttgaccgt ttccaagcta agtgcctcca aatccaactc
    20641 ttcatctagg tttaacttat tcttgccacc agcatctgca cctttttcac gaacataatc
    20701 ttccaaaacc tgagataaat ctgagagttg ctcaacttcc agcgtcatac ccgctgcacc
    20761 tgcatgacct ccaaaggcga tgaagaggtc tcgatgggga tccagagctt caaaaatatc
    20821 gaccgcttcc acactacgag cactgccctt ggcacgaccg tcttctatat taagaacaat
    20881 gactgtctgt cccaattctt ccaataaacg accagccacg attcctagaa ccccaggatt
    20941 ccagccttcc ttggccaaga cctgaacctt cttctcagga tccaccatgg tcttggcttc
    21001 ttcatagata gactgaacga tttccttgcg ctcttcgttt ttctggtgaa tcataagggc
    21061 aatctcatgc gcttcctcat catcaaatcc agtcaacaaa tcaatggcag gattgggatc
    21121 atccaagcga cccaaggcat tcaaacgagg agcaatctgg aaaccaaccg tttcttctgt
    21181 tacttcgttg gcagcaatcc cagccatgtc cagcatttct tgcagaccaa tgcgctgggt
    21241 atgacccaac atttccagac catattgaac taagatacga ttttcatccg tcagactcac
    21301 catatctgca atagttccaa tagcgaccaa atcaagcaat tccacttgca cttcttctaa
    21361 cagggcacaa gccaacttga aagcaactcc acaaccagcc aaatatttaa aaggataatc
    21421 cgcatctgga tgttcaggat ggacaatagc ataagcatct ggcagggttt caggcatgga
    21481 atgatggtct gtcacaatga catctactcc catagactga gccaatgcaa tagcctcatg
    21541 accagcaacc ccattgtcca ccgtcacaat caaggaaatc ccttcttgct cgataaagta
    21601 tttataaaca ctagcattag ggccatagcc atcggtaaaa cgatttggca ggtaaactcg
    21661 gcactcagca ccaagttgtt ccaaactttc cttcacaata gaagccgaag tcatgccatc
    21721 cgcatcatag tctccataaa caagaatatt ttccccttct tcaatagcct gacgaatccg
    21781 ctccactgcc ttgtccatat catggagcag ataagcatca tgtaagtcct ccaaggaagg
    21841 ttctaaaaac ttcttcagac tttcttggtt ctgaatccct ctctcaaaca ataaccgagc
    21901 cacctcagga cccagtccag ccttcttggc tatctttgta aaatccgcat cttctacctg
    21961 cagggcaaac tgccattcat aagtaggtgt tatcaaaaag acatccactc tttctaagaa
    22021 ttctattgct tcattataac atgatttaga cgttttctct atccagattg cttttttata
    22081 acattttata gtatattgaa actagaatag tacacctcta cttctaaaat attgttagaa
    22141 atcgatttga ctgtcccgat cgatttttcc tgttcttatt tcactatagt gaattttttc
    22201 agatatgatt tgacaagaac attcttttat tgtagaaccg tgttataaaa tataacacaa
    22261 tagagatctc tcatggcttt agtagaattt aaaaacgtcg aaaaatatta cggagactac
    22321 cacgcattcc gcaacatcaa tctccgtttt gaaaaaggac aagttgttgt cctgcttgga
    22381 ccttctggct ctgggaagtc cactcttatc cgtacgatca atggtttaga gactgttgac
    22441 aaaggaagtc tcctagtcaa tgggcaccaa gttgctggtg ccagccagaa agatttggta
    22501 cctcttcgca aggaagtcgg catggttttt caacatttta acctttatcc acacaaagct
    22561 gtgttagaaa acgtaacgct tgcacccatt gaagttctag gaattgataa aaaagaagct
    22621 gaaaaaaccg cccaaaaata tctggaattt gtaaatatgt gggacaagaa agattcctat
    22681 cccgccatgc tatctggtgg acaaaaacag cggatcgcca tcgctcgtgg tcttgctatg
    22741 catccggaac tcctcctctt tgatgaacca acatctgctc ttgatcctga gactatcgga
    22801 gatgttctag cagttatgca gaaactggcg catgatggga tgaacatgat catcgttacc
    22861 cacgaaatgg gctttgctcg agaggttgcg gaccgcatta tctttatggc cgacggagaa
    22921 gttttagtag atacgacaga tgtcgataac ttttttgaca atccaagcga acctcgtgcc
    22981 caacaattcc tcagcaaaat tatcaaccac gaaagtgaca aagtcaaata aggaggcgcc
    23041 tatgaaaaaa aattcttttt atcagcatta ttgattagcc ttttcggcct tgctgctgcc
    23101 aaaccagtcc aggctgatac aagtatcgca gacattcaaa aaagaggcga actggttgtc
    23161 ggtgtcaaac aagacgttcc caattttggt tacaaagatc ccaagaccgg tacttattct
    23221 ggtatcgaaa ccgacttggc caagatggta gctgatgaac tcaaggtcaa gattcgctat
    23281 gtgccggtta cagcacaaac ccgcggcccc cttctagaca atgaacaggt cgatatggat
    23341 atcgcgacct ttaccatcac ggacgaacgc aaaaaactct acaactttac cagtccctac
    23401 tacacagacg cttctggatt tttggtcaat aaatctgcca aaatcaaaaa gattgaggac
    23461 ctaaacggca aaaccatcgg agtcgcccaa ggttctatca cccaacgcct gattactgaa
    23521 ctgggtaaaa agaaaggtct gaagtttaaa ttcgtcgaac ttggttccta cccagaattg
    23581 attacttccc tgcacgctca tcgtatcgat accttttccg ttgaccgctc tattctatct
    23641 ggctacacta gtaaacggac agcactacta gatgatagtt tcaagccatc tgactacggt
    23701 attgttacca agaaatcaaa tacagagctc aacgactatc ttgataactt ggttactaaa
    23761 tggagcaagg atggtagttt gcagaaactt tatgaccgtt acaagctcaa accatctagc
    23821 catactgcag attaaggagg acaccccatg acagatttat catcttggac agcctatttt
    23881 caggattttg gacaattttt caatggtttc ctcttcaccc ttgccctagc ggttggttcc
    23941 tttatcctcg ctatggtttt gggcatcttc tttggtgcca tgtcaaccag taaacgtcca
    24001 attttacgca ttttagctcg catctttgtt gaattttacc aaaatactcc cctcttggtg
    24061 cagtttgtta ttgtttttta tggcctacct cttatcagtg accatatcat catgattcca
    24121 atttattgga cagccgttct ctgcgtggga ctctatcacg gcgcttatat cgctgaggtt
    24181 attcgttcag ggattcagtc tattcctagt ggtcagatgg aggccgcctt gtcgcaaggt
    24241 tttacctata tcagtgccat gcgcttgatt atcttgcctc aggccttccg catcattctc
    24301 cctccattga ccaaccaaat tgttaacctc atcaagaaca cctctactgt agctatcatc
    24361 tctggagtag acttgatgtt tgtgactaag tcttggtcgg ctctcaacgg aaactatata
    24421 ccagcctttt taggtgctgc tcttctctac tttgctctat gcttccctgt tgcccagttt
    24481 ggtcgcaaga tggagcaagc caacaaaaaa gcctattcac tttaggaggt tactatggaa
    24541 tctattttag aagttttaac cccagataac ctagtcttta tctttaaagg atttggcttg
    24601 accctctata tttctctgat tgccatcatc ctctctacta tcatcggtac ggtgctagct
    24661 gtcacgagaa atggcaaaaa tcctgtctta cgcattattt ccagtattta tatcgagttt
    24721 gtgcgcaacg ttcccaacct tctctggatt tttactatct ttttggtgtt caaaatgaaa
    24781 tccacaccag caggtattac agcctttact ctctttacat cagcagcctt ggctgagatt
    24841 attcgaggcg gtctcaatgc cgtagacaag ggacagtacg aagcaggaat gtcacaaggc
    24901 ttcacctcag cccaaatcct ctactacatc attctcccac aagccatccg caaaatgcta
    24961 ccagccatca tttctcagtt tgttaccgtg attaaggata ccagtctcct ctactctgtt
    25021 atcgccctac aagaactctt tggagccagc caaattctca tgggccgtta tttcgaacca
    25081 gagcaggtct tcagtcttta catcctgatt gccctcatct acttcagctt taacctagca
    25141 atttctagcc tgtctcatat gctagccaaa cgttggcaac aagctgcaga ataacactct
    25201 tcgaaaatct cttcaaacca cgtcagctct atctgcaacc tcaaaacagt gttttgagca
    25261 acttgcggct agcttcctag tttgctcttt gattttcatt gagtataaac agccactctc
    25321 cagttcaatt ggagagtttt ttgatgagag tagatattta tagacctcaa tttgacacca
    25381 tacattttct atgataaaat gtaacaaatt tatttacaac aaatcgaaat ctaaatgaaa
    25441 tggaattttc tatgaaatca gtcaaaggac tactctttat catagctagt tttatcttga
    25501 ctcttttgac ttggatgaac acttctcccc aattcatgat tccaggacta gctttaacaa
    25561 gcctatctct gacttttatc ctagccactc gtctcccact actagaaagc tggtttcaca
    25621 gtttggagaa ggtctacacc gtccacaaat tcacagcctt tctctcaatc atcctactaa
    25681 tctttcataa ctttagtatg ggcggtttgt ggggctctcg cttagctgct cagtttggca
    25741 atcttgccat ctatatcttt gccagcatca tccttgtcgc ctatttaggc aaatacatcc
    25801 aatacgaagc ttggcgatgg attcaccgcc tggtttacct agcctatatt ttaggactct
    25861 ttcacatcta catgataatg ggcaatcgtc tccttacatt taatcttcta agttttcttg
    25921 ttggtagcta tgccctttta ggcttactag ctggttttta tatcattttt ctatatcaaa
    25981 agatttcctt cccctatcta gggaaaatta cccatctcaa acgcttaaat cacgatacta
    26041 gagaaattca aatccatctt agcagacctt tcaactatca atcaggacaa tttgcctttc
    26101 taaagatttt ccaagaaggc tttgaaagtg ctccgcatcc cttttctatc tcaggaggtc
    26161 atggtcaaac tctttacttt actgttaaaa cttcaggcga ccataccaag aatatctatg
    26221 ataatcttca agccggcagc aaagtaaccc tagacagagc ttacggacac atgatcatag
    26281 aagaaggacg agaaaatcag gtttggattg ctggaggtat tgggatcacc cccttcatct
    26341 cttacatccg tgaacatcct attttagata aacaggttca cttctactat agcttccgtg
    26401 gagatgaaaa tgcagtctac ctagatttac tccgtaacta tgctcagaaa aatcctaatt
    26461 ttgaactcca tctaatcgac agtacgaaag acggctatct taattttgaa caaaaagaag
    26521 tgcccgaaca tgcaaccgtc tatatgtgtg gtcctatttc tatgatgaag gcacttgcca
    26581 aacagattaa gaaacaaaat ccaaaaacag agcttattta cgaaggattc aagttcaaat
    26641 aagtaatcat actctcctgg gatttcccag gagagttttt tatagccaaa tgaaataaga
    26701 caggaacgat ggaattttgg cattcaaatc aattgataag actattttat agtggagtga
    26761 aataggatac gaacaaattg attaggaaaa tcaaatgaat ttatagaaat cttttagcag
    26821 ttatgttatc ctattctagt ttcaaaacgc tatagaagca gcattgtgct agtctagatt
    26881 cagtttacta tactaaaacg agtagcttga aatcaaaaaa cccaccctca caggcaggtt
    26941 ttatctgtat tattcagcta gattatgctt taccttctga accgaatacg tcgatacgtt
    27001 cttcaaccga tgcttggata gcttttacac cgtcagcaaa gaatttacgt gggtcgaaga
    27061 gttttttctt gtcgtattct gcttcgtttg cttcgtagtc acgagcaaat ttacgagttg
    27121 cgttagcgaa tgcgatttgg cattctgtgt taacgttaac tttggcaaca ccaagtttga
    27181 tagctgcttg gatttgctca tcaggaatac ctgatccacc gtgcaatacg attgggaatc
    27241 ctggaagagc ttctgtcaat ttttgcaagt ggtcaaggtc aagaccttcc cagtttactg
    27301 ggtaaggacc gtggatgtta ccgataccag ctgccaagaa gtcgatacca gtttcaacca
    27361 ttgctttagc gtcttcgatt ggagccaatt cacctttacc gatgattcca tcttcttcac
    27421 caccgatagt accaacttca gcttctactg agataccttt agcgtgtgct ttttcaacaa
    27481 cttctttagc caatttaagg ttttcttcaa ctggaaggtg tgaaccgtca aacatgattg
    27541 aagtataacc aacttcgata cactcaagtg catcttcgta gtgaccgtgg tcaaggtgga
    27601 tagctactgg tacagtgata cccattgatt caacaaggtt agcgatcaag ttgcgagcaa
    27661 ctttgtaacc acccatgtat ttagcagcac ccattgaagt ttggatcaaa actggagctt
    27721 ttttagcttc tgctgcgcgc aagatagctt gagtccactc aaggttgttt gtgttaaatc
    27781 caccaactgc ataaccgttg tcacgggctg cttggacaaa tttttctgct gaaacgattg
    27841 ccattttatc aggcctcctg tatattttta tgggtcatcc catttacatt gttcatttta
    27901 tcactttttg ccaaaaaaat ctagtttttc ccgcagtttc gattgatttt cttctaactc
    27961 catctatgta aaccctttct ctccctagtc ttggacgact tttggaaaat ctataaagaa
    28021 ggttaaacta ttctcctcca tctcgaaacg ataagctaat ttttcatgtt ctaatagact
    28081 cttaaccaca aagagcccca taccagaccc cttgaccttg cgactggcat tgtcagaaaa
    28141 agactgggct agtttttctt gttcctctga gctacagcta ttttcgataa aaagttctcc
    28201 ttctctttct ccaattcgaa ctaagccacc tggaacagag tgcttaatgg cattgctgat
    28261 gagattagaa agaatcaact tcataactga tgggtttaga taagcctgct gatgggtcaa
    28321 actattgtct atctggagct ctctttcctt ggctagcaag gcataatctt tgaccagatt
    28381 ttgcgtcatc tggaggaggt caattgtttc cctatcatct cgcaattcct gcacagaaga
    28441 gagggaaagt atctgcagaa catggtgatt gagttcatcc acaatcccca aggcaactcc
    28501 cagatactgg tctctatcct tataacgacc gatattctct ctcatatttt cgattaggat
    28561 tttcaaacta gccagcggtg ttttcaattc atgagaagct cctcgtagga attcgacctt
    28621 catcttctcc agctggagaa tggcttcatt cttttcatgc aagtccgcaa taacagtcaa
    28681 gagatgctgg tagaggctat tgatttgttc cttgagatta cctatctcat ccttagaatc
    28741 cacgcgcaat cgcacttggg aatccaggtc catcatccga cgggtcaccc gcttgatttc
    28801 caaaatcggt gcaacaatag tccgagcgta gatgtaggcc accaaaaggg aaatcagaaa
    28861 ggaggccagc aaggtatagg gaagaaactg gagactgatt tgctccgctt ccttttgtaa
    28921 atccatggaa gctagaaact ggagaatcat agtaccaccg tcttgcgttt tcacctcgcg
    28981 ctcctcaata aagagagagg ttgtctggcg gtctgtgtcc agaggaagac tgtccttgac
    29041 ttctaacttg tcctcggtca tctcaccttt gacggtcccc ttgatatcac tagtctggga
    29101 atacaagtct aacacttgct cgatactctg cctatctttc ccttctaggg actgggcaat
    29161 ggctgttgcc ttttgaccaa tggtttcctg acgatgactc agataagtcg aaggaaaaag
    29221 aaaataaata gctaaatgaa ggcagataac cagaacacta aatatcgaga aggtatagat
    29281 aaatatcttt gcaaataaac ctgttcgttt cattttcgct ccaatttata accaacattg
    29341 cgcacagtga ggatacaatc caagtctagc tttttccgca attccttgat ataaacatca
    29401 ataacacggt caaagggaac ctcatctgtc gctttccaga cggcatcgat aatctgagat
    29461 cgagtcaagg cccggccttc atttttcact agatagtcca gaatttccaa ctctttggca
    29521 ttgataggca cttcttgacc tgcgaggctt gcactgtagc tttcaaagtc caccttggta
    29581 tccttgtaag aaaagattcg tcctgtatcg tagtagcgct tgaaaatcgc gtccaccctc
    29641 acttttaaaa gggagaggga gaaaggtttt tccagatagc catctgccaa agaggcaaag
    29701 gcactcatct tgtattcctc atcttgaaaa gctgtcaaca tcaagacagg aacctgactg
    29761 gttttacgaa tctcagctag gacttctaag ccgttgagct tgggcatctg gatatccagt
    29821 aaaaccaggg ccacctcata gctagaaaat tgctccagag cttcctgacc gtccgctgcc
    29881 tcaatagttt catagccaca atccgtcaaa taatcactga ccccctcacg gatcatctct
    29941 tcatcttcta caattaaaat tttcatactt taactgctct ctatttttta tttttcttag
    30001 aataaatacc taccctattt tctattatag tctcttgctg gccttttgtc tgcaagcaac
    30061 tgaccactag ataaaacgtt gtgaaattcc tttctcataa attccataac tttagtatat
    30121 tatatttaag cactaaagta caaagaaagc aactgaaagc aatgattttc accactgctt
    30181 tcggatttat tttgaattgt taaatagcca ttcctatcca ctattcttga atagaaacac
    30241 aagatgcaat ctttattcta gactcatttt ttcaaattta ttcaccatcc agcaagagct
    30301 cttttggttg ttttctaagg agattgcttg aagcaagcgc cataacgaga accactagaa
    30361 ccaaggcaag gacaaaaatg atgataaagt ctgatgtctg aatggaaatg tctaggctcg
    30421 acaaggtctt gctaaagcca tctacttctg caccaccacc aaggttagag gcttgagccg
    30481 ccttactagc ctgtttggca acacctgaag tcacattggc aaggacagtg tttccaattg
    30541 cacgggcagt gtaattagct aggaagtaag cagaaactag agcagggata gcaatcaaga
    30601 tagattcggt gatgaattga cccaagatac ttgcctgctt gaggccgata gagaggagaa
    30661 ttcccacttc cttgcgacgg gcgttgatcc aaaggctgag caagagggca aggaggagaa
    30721 ctgagaagct caagctaccc cagaagagga ggttggccat cttgtacata ccagagatag
    30781 attgctcaag agctgggtag ttagaggagc tcttgacgag tgtgtagctc ttccagttga
    30841 taccactgat gccattcaac tctttcataa catcatccaa gttcttgtct gctgttacaa
    30901 agaaggttgc gtccccataa atggctgtgt cttctgtgta tccataaagt tttgcagcag
    30961 tgtgaatgtc tgtaatagct gtgttttcgt aaagttcttg tgagtaggtt actgctgact
    31021 tattatgacc atcaaagagt cccttgattg tcacttcaac tgtttccttg gctccttttt
    31081 cattatctgc atcgtagata ttagagtcca gtttaacctt gtcccctact ttccagccgt
    31141 gtttggctgc caagtccttg tgcaagagga ttttatcctt gtcgtcgttg gttaagtgct
    31201 ctccttcgac tagtttataa gaaccagaga caaacttgtc ttctttagag gagtcattga
    31261 cacctgtaat catcaagcta cttccaaaac gcttggcacg atcagcagtg agattcttct
    31321 tggtttctgg cgtttcaatc aggtcatatc cagtcaaatc tccgatagcg ttgatacgtt
    31381 tgacataaga ctcaatggcc ttgttttcgg tgattttttt gatgtcttca cccttgatat
    31441 tcccagcacc acgaggcgtt ccttggttga cgcgacgatt gatttgcatg gagaagctat
    31501 tggtgatatt tttaaaggtc tcctgagaag ccttggcagt agctcccttg attgacaagc
    31561 cgaccaaact caagctcgcc atgaggagaa taatcaggaa gatgacaatc gatttgaaaa
    31621 acttccttgt aacataggca aatgcgttgt gtaacataga ttccctttct agattttgtt
    31681 ttaatcattc tattaaaata agctcaaatt atttactagt attgcgcgtt tcagtcagtt
    31741 tcttatcctt taattcaagt gtaatatctg acgcttgtgc cacttcttta ctgtgagtta
    31801 cgacaatcac acatttacct gttttctggg caagtgattt gagtagttcg acaatatctc
    31861 cagcagtttt aggatccaga tttcctgttg gctcatcagc tagaataact ggagcttctg
    31921 agaccaaact gcgagcaatg gcaacacgtt gctgttgacc acctgataac tggagaacat
    31981 tccgcttgat ctggctttca tccaaaccaa gctcaagaag tgtattcttg cttgcctttt
    32041 tgttgaccaa tcggatattt tccagcggag aaagataatc tatcaagtta taattttgaa
    32101 agaccaggga aatatggtgc atgcgatggt aagaatagcc cttcttacga atatcctctc
    32161 cttgaaaaag gatagaacct tcaacaggac tatctagacc agcaagtagg gacaagagtg
    32221 tggattttcc tgctcctgac tccccaataa tactgtaaaa ttttccgggt tcaaaattat
    32281 aattgatctg atataggact gcttcagcag tattcttata acggtaggta acatcttgta
    32341 attgtaataa agtcatgatt tctccttctt aactaataga tgataaaatt tctttcggtg
    32401 attttctaaa taagaatagg aaacaaaggg ctacagataa gcaactaagc agaactagaa
    32461 aaacatagga ttctgcaaaa gataagatgc tagttgataa actgcttgct ttggctagtg
    32521 tatcttgtaa gcttgcctga tctccacttg ctagtagagt ttggagtagg taagttgtga
    32581 ttgcgtttcc tgcaacaaat gctggaagca aagctccaag agataccaaa actacctcta
    32641 aacagaattg taggaagatc gagctcttgc cttttccaag tgcaagtaaa atccccactt
    32701 catagacccg ttctctcaac cagagagaca aaaccagaat taaggctcca gctcctgcta
    32761 tcaacatccc ataaaggaag atggtcagga aggtttggaa agttgcaact gagtctttga
    32821 tttgttcaaa agccttgttt tccttttcga cttggtagcc ttgattttcc aaggccaagt
    32881 tttctacctg cttcatgagt ccgtccattt ccttaggatt ttctacatag aagcgtgctg
    32941 cactgacttg agcttcacta ttgcccaaaa gggtttggct actttcatag tctgtaaaga
    33001 cttgattttc actgaagtca gaagacaagc ctgtgaattt ctcttgtttt ttaccagaaa
    33061 agatgccgat aatctcaaac tctactgttt gtccttttcc agattcagac tgaccagcat
    33121 ccaagccaat cttgtcatga agcgaaagac cgttcttctt agccaattct tcgtggataa
    33181 ggattttctt ggaatcccct ttttgaaggt gtcgcccttc ttttagattg aaagccgaac
    33241 tggtaaaggt tacatccttg gatgaatcct caagagccgt taagctaacc aagttattgt
    33301 ctgcagctga taaatcatca cgctccacgc tctgctcgcc agtcactgct tccttgtctt
    33361 ttagttttgc gaccgtctca agttcaggag agacattttc cagcccctta atcttgctta
    33421 cagatgctag gtctgacaac ttgaatgtct gaccattctc tatcttctta atagaaaaag
    33481 atgtattgag tgatttataa agattgcttt ctactgtttt gttggacttc atcagagtca
    33541 aacaggctga aattccggcc aataagacca ataaaatcag aaataaaata aaacttctca
    33601 gtcgctttct gctgacataa gcccaagatc tttggattgg attcatttgt cacctccata
    33661 tttgtaagac tattataaaa cccaaatatg aaatgtttat gaaatacgaa aaaaaaatat
    33721 cgagtagggg ataatctcta gcccctctca caccacc
//
LOCUS       DBEROU010000023        33031 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.023, whole genome shotgun sequence.
ACCESSION   DBEROU010000023 DBEROU010000000
VERSION     DBEROU010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 33031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.023"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(157..924)
                     /locus_tag="ACL8CL_001147"
     CDS             complement(157..924)
                     /locus_tag="ACL8CL_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HGS1330399.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(921..1787)
                     /gene="accD"
                     /locus_tag="ACL8CL_001148"
     CDS             complement(921..1787)
                     /gene="accD"
                     /locus_tag="ACL8CL_001148"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HGS1330400.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(1824..3191)
                     /gene="accC"
                     /locus_tag="ACL8CL_001149"
     CDS             complement(1824..3191)
                     /gene="accC"
                     /locus_tag="ACL8CL_001149"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HGS1330401.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(3203..3625)
                     /gene="fabZ"
                     /locus_tag="ACL8CL_001150"
     CDS             complement(3203..3625)
                     /gene="fabZ"
                     /locus_tag="ACL8CL_001150"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HGS1330402.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGILTFAIGN"
     gene            complement(3622..4107)
                     /gene="accB"
                     /locus_tag="ACL8CL_001151"
     CDS             complement(3622..4107)
                     /gene="accB"
                     /locus_tag="ACL8CL_001151"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HGS1330403.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLATPSPVAPTSAPAETVAEEVPAPAEASVATEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(4110..5345)
                     /gene="fabF"
                     /locus_tag="ACL8CL_001152"
     CDS             complement(4110..5345)
                     /gene="fabF"
                     /locus_tag="ACL8CL_001152"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HGS1330404.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(5367..6098)
                     /gene="fabG"
                     /locus_tag="ACL8CL_001153"
     CDS             complement(5367..6098)
                     /gene="fabG"
                     /locus_tag="ACL8CL_001153"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HGS1330405.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQVGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVIAIDGGLSM"
     gene            complement(6132..7052)
                     /gene="fabD"
                     /locus_tag="ACL8CL_001154"
     CDS             complement(6132..7052)
                     /gene="fabD"
                     /locus_tag="ACL8CL_001154"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HGS1330406.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(7045..8019)
                     /gene="fabK"
                     /locus_tag="ACL8CL_001155"
     CDS             complement(7045..8019)
                     /gene="fabK"
                     /locus_tag="ACL8CL_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HGS1330407.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     ISIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVD
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWAGVVRND"
     gene            complement(8138..8362)
                     /locus_tag="ACL8CL_001156"
     CDS             complement(8138..8362)
                     /locus_tag="ACL8CL_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002938891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGS1330408.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(8422..9396)
                     /locus_tag="ACL8CL_001157"
     CDS             complement(8422..9396)
                     /locus_tag="ACL8CL_001157"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HGS1330409.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGKELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(9396..9830)
                     /locus_tag="ACL8CL_001158"
     CDS             complement(9396..9830)
                     /locus_tag="ACL8CL_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HGS1330410.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(9924..10709)
                     /locus_tag="ACL8CL_001159"
     CDS             complement(9924..10709)
                     /locus_tag="ACL8CL_001159"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HGS1330411.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRVSSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            11078..12442
                     /locus_tag="ACL8CL_001160"
     CDS             11078..12442
                     /locus_tag="ACL8CL_001160"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HGS1330412.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQICTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREIGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            12445..12816
                     /locus_tag="ACL8CL_001161"
     CDS             12445..12816
                     /locus_tag="ACL8CL_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HGS1330413.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            13029..14303
                     /gene="serS"
                     /locus_tag="ACL8CL_001162"
     CDS             13029..14303
                     /gene="serS"
                     /locus_tag="ACL8CL_001162"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HGS1330414.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(14328..15227)
                     /locus_tag="ACL8CL_001163"
     CDS             complement(14328..15227)
                     /locus_tag="ACL8CL_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HGS1330415.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLAE
                     TIICLTILFCVLGAYLIWLRKKV"
     gene            complement(15337..15885)
                     /locus_tag="ACL8CL_001164"
     CDS             complement(15337..15885)
                     /locus_tag="ACL8CL_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HGS1330416.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(16103..17425)
                     /locus_tag="ACL8CL_001165"
     CDS             complement(16103..17425)
                     /locus_tag="ACL8CL_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HGS1330417.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSIAESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMAIIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILVTGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGVRFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMVLPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(17516..17604)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(17768..20104)
                     /locus_tag="ACL8CL_001166"
     CDS             complement(17768..20104)
                     /locus_tag="ACL8CL_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HGS1330418.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QNFYTNLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVYFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(20208..20756)
                     /locus_tag="ACL8CL_001167"
     CDS             complement(20208..20756)
                     /locus_tag="ACL8CL_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HGS1330419.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPITTSWLKQIWVTNLIG"
     gene            complement(20753..21055)
                     /gene="zapA"
                     /locus_tag="ACL8CL_001168"
     CDS             complement(20753..21055)
                     /gene="zapA"
                     /locus_tag="ACL8CL_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HGS1330420.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            21142..22023
                     /gene="rnhC"
                     /locus_tag="ACL8CL_001169"
     CDS             21142..22023
                     /gene="rnhC"
                     /locus_tag="ACL8CL_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HGS1330421.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQLVEENRGQNLPLIGTDEVGNGSYFGGLAVVASFV
                     TPDQHAFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANCFSNPISLEEKAEG
                     KYLAVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            22027..22641
                     /gene="lepB"
                     /locus_tag="ACL8CL_001170"
     CDS             22027..22641
                     /gene="lepB"
                     /locus_tag="ACL8CL_001170"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HGS1330422.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            22721..22843
                     /locus_tag="ACL8CL_001171"
     CDS             22721..22843
                     /locus_tag="ACL8CL_001171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330423.1"
                     /translation="MKIKEQTRKLAVGCLKQCFEVVDRTDEVSSKYCFEVADGS"
     gene            22920..25286
                     /locus_tag="ACL8CL_001172"
     CDS             22920..25286
                     /locus_tag="ACL8CL_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0043139 - 5'-3' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HGS1330424.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGIAGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVHILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDLRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDADFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSEESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKGDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDSIPAMIGITDTDLKEIFGK"
     gene            complement(25334..26617)
                     /gene="tig"
                     /locus_tag="ACL8CL_001173"
     CDS             complement(25334..26617)
                     /gene="tig"
                     /locus_tag="ACL8CL_001173"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HGS1330425.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDQKFGEEALYQDAMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQSLLSADMLKHDITIKKAVELITSTATVK"
     gene            complement(27416..28552)
                     /locus_tag="ACL8CL_001174"
     CDS             complement(27416..28552)
                     /locus_tag="ACL8CL_001174"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HGS1330426.1"
                     /translation="MKHSVHFGAGNIGRGFIGEILFKNGFHIDFVDVNNQIIHALNEK
                     GKYEIEIAQKGQSRIEVTNVAGINSKEHPEQVIEAIQKTDIITTAIGPNILPFIAELL
                     AKGIEARRVAGNTQALDVMACENMIGGSQFLYQEVKKYLSPEGLTFADNYIGFPNAAV
                     DRIVPAQSHEDSLFVVVEPFNEWVVETKRLKNPDLRLKDVHYEEDLEPFIERKLFSVN
                     SGHATSAYIGAHYGAKTILEALQNPNIKSRIESVLAEIRSLLIAKWNFDKKELENYHK
                     VIIERFENPFIADEVSRVARTPIRKLGYNERFIRPIRELKELSLSYKNLLKTVGYAFD
                     YRDVNDEESIRLGELLAKQSVKDVVIQVTGLDDQELIEQIVEYI"
     gene            complement(28614..29051)
                     /locus_tag="ACL8CL_001175"
     CDS             complement(28614..29051)
                     /locus_tag="ACL8CL_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1330427.1"
                     /translation="MKLEKHLIKLNKQFSNKEEAICYCGQVLYEGGYVNEDYIEAMIE
                     RDKELSVYMGNFIAIPHGTDAAKKDVLKSGITVVQVPIGVDFGNVSNPQVATVLFGIA
                     GIGNEHLEIIQKISIFCADVDNVLKLADAQSKEEVLRLFDAVE"
     gene            complement(29053..31008)
                     /locus_tag="ACL8CL_001176"
     CDS             complement(29053..31008)
                     /locus_tag="ACL8CL_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HGS1330428.1"
                     /translation="MLLTKREEQLLKAFLHVGKLSMQDMTEILQVSSRTIYRTLSDLT
                     DSMEQYGIEITKHGKYYILTGELDDLPTELEVLVEYSPQERQELITYRLLTESGFVTN
                     EALQECTKVSNVTIIQDISDIDKRLLDFDLKIERQKGYRISGDSVGKRRFLAILLTNC
                     ISVADFSTGNFGSFDILEADRTRLASQIVNKQLSGFPDMDARMKMFFAILLSLIGQEQ
                     NIENSPNTSKQALEISQKIFQAYSKQTAQFYSIQEIIYFASILDELIIKRQDNPLFTE
                     KFDGEFFYNISNLIDTVSMYTKIDFFKDKVLFNFLFHHIRLSLGVPILFQGENLPESV
                     QILVERNKFLYTVISLLVNDIFPKYLHTEYEYGMIALHFISSLGRSPEIYPVRVLLLT
                     DERRVTRDLLVSKIKSVAPFVELIDIQSLVDYHSIDLSQYDYILSTKPLTNQEIDVIS
                     SFPTVKELLELQERLQYVQAHRAIVARDAIAPEKSYDLQDYLISSSQLLSQFELVQLE
                     NNQSFEHTVEQIIQYQKNVSDRDYLTRKLLSHFQNSPMAIPNTGLVLLHSQSSKVTTN
                     SFTMFELKLPISALSMKREEEEVKRCLLMLMSKEASEEARDLMTAISQSIIENHLYTE
                     IYKTGNQSIIYQMLNTIFNEKIKKLEN"
     gene            complement(31032..32801)
                     /locus_tag="ACL8CL_001177"
     CDS             complement(31032..32801)
                     /locus_tag="ACL8CL_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol-specific transporter subunit IIBC"
                     /protein_id="HGS1330429.1"
                     /translation="MEEKVSLKVRVQKLGTSLSNMVMPNIGAFIAWGVLTALFIADGY
                     LPNEQLATVVGPMLTYLLPILIGYTGGYMIHGQRGAVVGAIATVGAITGSSVPMFIGA
                     MVMGPLGGWTIKKFDEKFQEKIRPGFEMLVNNFSAGLVGFALLLLAFYAIGPVVSTLT
                     GAVGNGVEAIVNARLLPMANIIIEPAKVLFLNNALNHGIFTPLGVEQVAQAGKSILFL
                     LEANPGPGLGILLAYAVFGKGSAKSSSWGAMVIHFFGGIHEIYFPYVMMKPTLFLAAM
                     AGGISGTFTFQLLDAGLKSPASPGSIIAIMATAPKGVWPHLNVLLGVLVAAVVSFLVA
                     ALILHADKSTEDSLEAAQAATQAAKAQSKGQLVSTSVDAVVSTDSVEKIIFACDAGMG
                     SSAMGASILRDKVKKAGLEIPVSNQAISNLLDTPKTLIVTQEELTPRAKDKSPSAIHV
                     SVDNFLGSPRYDEIVASLTGASPIAEIEGDIPTSAPVDSQEIDLNHIDAVVVAYGKAQ
                     GTATMGCETIRAIFRNKNIRIPVSTAKISELGEFNSKNIMIVTTISLQAEVQQAAPNS
                     QFLIVDSLVTTPEYDKMAARMYK"
BASE COUNT         9723 a         7460 c         6282 g         9566 t
ORIGIN      
        1 tatagattca atacactata aatttttgta agcttacatc tacagtatac atcttttttg
       61 tctattttat agcccatctc ctcaactgtc aatttttcaa cctgaattgc atttttccat
      121 aaaaaatgag acctttctag tctcatttag tcattcttag tattttctaa atcgttgata
      181 gcgttcttcc agcaactctt ctagcggttt ttgtgaaagt ctagccagct ccgtttggag
      241 ttcttttttg acactcttaa tcagttcttt actagaaagt cctacttcag aaatcacctt
      301 atccaccacg tccatttcta acagttcatg cgaagtgatt ttcatcagtt ctgctgcttc
      361 catggcgcga gtaccgtcct tccataaaat ggaagcaaag ccttctggac tgagaatggc
      421 atagatagaa ttttccagca tccagacacg gtccgcgaca gctagagcca gagccccgcc
      481 tgaaccacct tcaccgataa taatggcgat aataggaact ttcaggtcac tcatttccat
      541 gagattgcga gcgatagctt ccccttgacc acgttcttct gctccgacac caggataagc
      601 acctgctgta ttaataaagg tcacaactgg acggccaaat ttctcagcct gtttcatcaa
      661 ccgcagtgcc tttcggtagc cttctggatg tggttggcca aaattccgtt tgaggttgtc
      721 ttgcaaactc ttgccttttt ggataccaac aactgttaca gcttggtctc caagccaacc
      781 aataccacca acaactgcac catcatcacg aaaagaacgg tcaccatgta attggataaa
      841 ttcatcaaaa atgcctgtcg caaagtccaa ggttgtcaag cgactctgct cacgcgcttc
      901 tctgactatt tttgcaatat tcatctagga ctccctccat gcaatctgac taggctagca
      961 atcgtatctg gtaagtctct tcttttgaca atagcatcca caaagccatg ttctaatagg
     1021 aattctgcct tttggaaatc ctcaggcaag ctttcacgaa ccgtattttc aatcacacga
     1081 cgcccagcaa aaccaaccaa gctctgtggt tcagccagaa tgatatcgcc ttccatagcg
     1141 aaagaagctg tcacaccacc agtcgttgga tctgtcaaaa tggtcaggta aaagagacca
     1201 gcatttgaat gacgtttaac cgccgcagag atcttagcca tctgcatgag actcatgatt
     1261 ccttcctgca tacgggctcc accagaggct gtgaatagga caactggcaa tttttcgaca
     1321 gtcgcatact caaacaaacg agtgattttt tcacctacaa ccgtacccat agaagccatg
     1381 ataaagttag aatccataat cccaagagcc acagtctgac ctttaataag agcagttcct
     1441 gtcacaacgg cttcatgcag acctgttttt tcacgcatag atgccagttt cttttggtaa
     1501 ccagggaaat gcaagggatc cttgctttca atccctgtaa acaattcttt gaaggttccc
     1561 atatcaatcg tcaaagccaa gcgttcttgg gcagaaatac gaaaggtata gctacagtgc
     1621 ggacagatac gttcacttcc cagatccttc tgatagatgg tatgcttaca gcctggacac
     1681 tgggaaaata attcatctgg aacctctggc ttagcttgag gtttttccct aaccgaacga
     1741 ttgggattga ttcgaatata cttatctttt ttactaaata gagccattga ttcccctttt
     1801 cggtttaaac tcttaaagtc attttattct ttttcttgat atttaggtaa gaaggtttcc
     1861 atcaagaagg aagtatcata atccccagca atgacattgc gatctgaaat gaggtcaagc
     1921 tggaaatctg cattggtctg cactccttca atctctaatt catagagggc acgttgcatt
     1981 ttcatcaagg cgtcaaaacg attttcgccg tgtactatga ttttggcaat catactatca
     2041 taataaggcg gaatggtata acctggataa actgctgaat ccacgcgcaa gccaactcca
     2101 ccacttggca gatagagatt agtaatctta cctggacttg gagcaaagtt aaaggctggg
     2161 ttttctgcat tgatacgaca ctcgatggca tgaccgcgta ggacaatatc ttcttgctta
     2221 acagacaaag gctgacctgc cgcaatgcaa atctgttcct taacgatatc aacacctgaa
     2281 acaaactctg ttactggatg ttctacctga acacgagtat tcatctccat gaaatagaaa
     2341 ttgctacttg cttcatcaag aagaaattca atggttcctg cattctcata gccaacaaac
     2401 tctgccgctc gaacagcagc agcacctatt tcatgacgca gcgtttttcc gattgcaatc
     2461 gagggacttt cttccaaaac cttttggtta ttcctttgaa gagaacaatc ccgctcaccc
     2521 aagtgaatca catgtccatg ctcatcacct aggatttgaa cctcaatgtg ccgagctgga
     2581 tagataaccc gttctatgta catggcacca ttgccataat tggccttggc ctcactagag
     2641 gcagtttcaa aggcagaaac gaggtcatct ggtttttcaa ccttacgaat ccctttacca
     2701 cctccacctg ctgaagcctt gagcataaca ggatagccaa ttttttcagc aacaatcaaa
     2761 gcttcttcag agttatgcac ttctccatct gaacctggta taacaggcac acctgcttta
     2821 atcatctgag cacgcgcatt gatcttatcc cccatcatat ccataacatg accagatgga
     2881 ccgataaact tgatacctac ttcttcacac atggtcgcaa atttggaatt ttcactgaga
     2941 aatccaaaac cagggtgaat agcttctgcc tcagtcaaga ctgcagctga tagaactgca
     3001 ttaatattga gataagactc tgttgccttg ccaggaccaa tacaaactgc ttcatctgcc
     3061 aaaagcgtat gaagagcttc cttatcagca gttgaataaa ccgctaccgt cgcaatcccc
     3121 aattcacgtg ccgcacggat aatacgaacc gcaatttcac cacgattggc aattaaaatt
     3181 tttcgaaaca tggagaacct ccttagttcc caattgcaaa agtaaggata ccactggctg
     3241 caagcttgcc atccacttca gcctttgctt caaccacagc tatggtgcca cgacgtttta
     3301 caaaagtcgc tgtcataacc aattggtcgc ctggtacaac ttgcttcttg aacttaacct
     3361 tgtccatacc agcgtaaaag accagttttc ctttattttc aggttttgat aactccaaca
     3421 caccagcagt ttgcgccaag gcttccataa tcagaacacc tggcataact gggtattgag
     3481 gaaagtggcc gttaaagaaa ggttcgttaa tggtcacatt tttgatagca acaatggtat
     3541 cctcgctcac ttccaagaca cggtccacta gaagcatagg ataacggtgg ggaagagctt
     3601 ctttgattcc ttgaatatcg atcatttgat acgtaccaat cctttaccaa actcaaccat
     3661 ttcttcgtta gagacgagaa tttccgttac cacaccatcc ttaggagctg ggatttcatt
     3721 catgactttc atggcttcga taattaccaa tgtttgacct tttttgacac tatcaccaac
     3781 tgtaacgaag gcaggtttat ctggtccagc agccaagtaa accactccaa caagtggact
     3841 ctctacaaga tttccctcag tagccacact tgcttcagct ggagctggaa cttcttctgc
     3901 tacagtctct gctggagcag atgtaggagc tactggactc ggtgttgcta gaacgggtgc
     3961 tggagcgact tgagttgcaa cttcaggcac aggtctcgct tcattcttgc taaactgcaa
     4021 ctcatccgtc ccatttttat aagaaaattc tctcaaactt gactggtcaa attgagtcat
     4081 caagtcttta atatcgttta aattcatact tatctattct cccaacgttt gaaagcaaga
     4141 actgcattgt ggcctccaaa accaaaagta tttgaaatag cgtatggaat ttctttctcc
     4201 aagccttgtc cataaacgac attagcttcg atataatctg atacttcact tgtcccagct
     4261 gtcattggta caaagttatg gcgcatagct tcgatggtga cgatagcttc tactgcaccc
     4321 gcagccccca gcaaatgtcc tgtaaaagac ttggttgatg atacaggtac ttccttacca
     4381 agaacagcta cgatagctcc actttctcct ttttcattgg caggagttga cgttccgtga
     4441 gcattgacat aggctacttg ctctggagaa atctcagctt cttccaaggc tagtttgatg
     4501 gccttgatag ctccctgacc ttctggatgt ggagaagtca tgtggtaggc atcacaagta
     4561 tttccgtaac caaccacttc agccaggata gtagctccac gtttttcagc gtgttcaaga
     4621 ctttctaaaa ccaacatccc tgaaccttca cccataacaa acccattgcg atccttatca
     4681 aatgggatcg aagcacgagt tggatcctct gtagtagaga gagctgttaa ggcttggaaa
     4741 ccagcgatgg caaaaggtgt gatagaagct tctgttcctc ccaccaacat cacatcttgg
     4801 aaaccaaact taatggagcg gaaggcatcc ccaatcgcat catttgatga agagcaggca
     4861 gtattgatag atttacaaac accgtttgca ccaaaacgca tggctacatt cccagaagcc
     4921 atatttggta aagcttttgg aagagtcatt ggtttgacac gtttgggtcc tttttcatga
     4981 aggcgaagta cctgatcttc aatttccttg attccaccaa taccagatgc aacgataaca
     5041 ccaaaacgat ccctattaag agcctctaca tcaagattgg catggtttac agcctcttgg
     5101 gctgcataca aggcatataa agaatagtta tcaaaacggt tggtatcttt ttttacaaag
     5161 tatttatcga acggaaaatc ttggatttct gccgcattat gcacatcaaa gtcactatga
     5221 tcaaattttg taatgccacc aatgccgatt ttcccagttg ctaaactatt ccaaaattct
     5281 tctggtgtat ttccgattgg agatgttact ccataacctg ttaccactac tcgatttagt
     5341 ttcattcttt tcacctctag ctttcgctac atacttaagc caccatcaat ggcaatcact
     5401 tgaccagtta gataatcttg gcctgctaaa aatactgtca aatctgcaac ctgctctgcc
     5461 tgcccaaatt ctttcatcgg aatctgagct agtgtagctt ccttaatctt atctgataag
     5521 atagctgtca tatcagactc aatcattcct ggagcaatca cattgactcg tatattccga
     5581 ctagcgacct cgcgtgccac agacttggta aagccaatca agccagcctt agaagcagca
     5641 tagttagctt gaccaatatt ccccatcaaa ccaacaacac tagacatatt aatgatagca
     5701 ccttctctgg ctttcatcat cggtttcaag actgattgtg tcatattaaa ggcaccagtc
     5761 agattgacct tgagcacttt ttcaaaatct gcttctgtca tcttgagcat aagagtatct
     5821 tgggtaatcc ctgcattgtt gaccaaaaca tctactgaac ccagttctgc aatagcttga
     5881 tcaatcatac gcttagcgtc tgcaaaatct gatacatctc ctgaaatggg aaccaccttg
     5941 ataccatagt ttgaaaactc agcgagcaat tcttctgaga ttgccccacg actgtttaag
     6001 acaatgttgg ctcctacttg agcaaacttg tgggcgatgg caagaccaat tccacgactc
     6061 gaacctgtaa taaagatatt tttatgttct agtttcattt ttttcctttc aaaacttcta
     6121 cttattttag tctatttttc taaaagtgct actaaactcg cttgatcttc cacatgagct
     6181 aagtgagcag tttgatcaat ttttttaaca aaacctgaca agactttccc cggtccaatc
     6241 tcgataaagt tgcttatgcc tgcttcttgc atgaccccaa tactttcata gaaacgaacg
     6301 ggttccttga cctgacgcgt caagagctga gcaatgtcct ctttttgcat cacagcagct
     6361 tctgtattgc cgactagggg acaagtaaaa tctgaaaaac ttacctgagc tagagtttca
     6421 gctagtttct ggctagcagg ctcaaggaga gcggtgtgaa agggacctga caccttaaga
     6481 ggaatcaagc gtttggcacc tgcttcttgc aaaagttcaa ccgctcgatc aactgcaacc
     6541 acttctccag caatgacgat ttgtgcaggt gtgttatagt tggctggagt aaccactcca
     6601 agttcagaag ctttttgaca ggcttcttca atgacctcta ctggcgtatt gagaactgct
     6661 accatcttgc cagagtcagc aggagccgct tcttccatat aggctccacg cttagctacc
     6721 aaggcaaccg catcttcaaa atccaaggcg ccgcttgcca ccaaggcaga gtattctcca
     6781 agagacaaac cagcaaccat atcaggctga tagccctttt cttgcaataa acggtagata
     6841 gcaaccgaag tcgctagaat ggctggttgc gtatagcggg tctgattgag tttgtcttct
     6901 tccgtatcga tgagataacg taaatcataa ccgagcacct gactcgctcg atcaatcgtt
     6961 tctttgacaa tcggatactg atcatagaaa tcccttccca tccctagata ctgggcacct
     7021 tgaccagcaa ataaaaaggc tgttttagtc atttcttaca actcctgccc agcgagaggc
     7081 ttcttcttga attttcttag cggctccgta atacaaatct tttaggattt cttcagctgt
     7141 ttcttcttta gaaacaagcc ctgcgatttg acctgccatg acagagccac catccacatc
     7201 accgtgaaca actgctttgg ctagggcacc tgctcccatt tgttcaaaga tttctaaatc
     7261 aggatcttcc tgcttaaagg catctttttc agccagttca aaatctctag tcaactgatt
     7321 tttaatagca cgaacagcat gaccaaagtg ctgagctgaa atcgtagtat caatatccct
     7381 tgcttttaaa attttctcct tgtagtttgg atgggcattc gactcttttg caactacaaa
     7441 ccgtgtcccc acctgtacag cctctgcacc tagcataaag ccagccgcag caccttcacc
     7501 atccgcaatt cctcctgcag caataacagg aatagatata gctgtggcta cctgtcgcac
     7561 caaggtcatg gttgttaatt taccgatatg ccccccagct tccattcctt ctgcaataac
     7621 agcgtctgca ccgatttttt ccatgcgttt agctaaagcg acactaggaa caacaggaat
     7681 aacgattatc ccagcttcat ggaaacgttc catatacttg cttggatttc ctgctcctgt
     7741 tgtgacaact ttaacacctt cttcaataac gagatccacg atatcttcca caaagggaga
     7801 taagagcatg atgttgaccc caaagggttt atcagtcaat gatttgattt tatcaatatt
     7861 ggccttgaca acttctttcg gggcatttcc cccaccgata attcctaatc ctccagcctt
     7921 ggaaacagcc cctgccaaat caccatcagc aacccaggcc atccctcctt ggaaaatagg
     7981 atagtcaatc ttcaataatt ctgtaatacg cgttttcata gtgcctccaa ccttccttgc
     8041 ttacgtaata gttcgatttc accataattt gacagtcaaa ctattaccta aacaagaggg
     8101 agtgggtttc tccctactcc ttctactaat attctgctta ttttgcttgc tcttcaacgt
     8161 aagcaaccaa gtcaccaact gttttcaagt cattttctgc ttcgatttgg atatcaaaag
     8221 catcttcgat ttctgagatt acttggaaca agtccaatga atctgcgtcc aaatcatcaa
     8281 aagttgattc aagtgttact tctgatgcgt cttttccaag ttcttcaacg ataatttctt
     8341 gtactttttc aaatactgcc atgataggac tcctttaaaa taaatagttt ttttataaca
     8401 atgtgttcac cacatgatta cctaaattgt aagaatgagc gtgccccagg tcaagcctcc
     8461 accgaagcct gatagaagaa cagtctggct accatctaaa gggatgagac cttgttctac
     8521 acactctgaa agtaaaatcg ggatactggc tgcactggta ttgccatatt ccatcatatt
     8581 ggctggaagt ttggctcggt caacaccaat ttttctagcc atcttatcca aaatacggtc
     8641 attggcttga tgaagtagca gataatccaa gtctgtcacc tctataggag attcatcaat
     8701 agtctgcttg atagacttgg ctacatctcg aatggcaaaa tcaaagactg tgcgtccatc
     8761 catcttcaaa aacgaatctg cactttcttg atctgaaaat ggagaatgca aacctgaatg
     8821 cccataagtt aaacactcgc tgcgacttcc atcgctattg agactctcag ctaagaaatg
     8881 ctcttgctcg ctagcttcta acaagacacc accagcacca tctccaaaca acacagctgt
     8941 tgatcgatcc gaccaatcga ctgccttaga gagggtttca ctaccaatca ccaagccttt
     9001 ttgaaagcga ccagaagcga taaacttttc agcagttgaa agagcaaata caaatccact
     9061 gcaagccgcg gttaagtcaa aagcaaaggc tttattagcg ccaatattag cttgaacacg
     9121 agcagctgta gagggcatca tcgaatctgg agtaatggta gctaggatga taaaatccag
     9181 ttcttttcct gttattccag cttttgccat cagtttctta gcaacctctg tagccaaatc
     9241 actggtagat tctgttcttg aaatatgcct ttgtcgtatt cccgttcgac ttgaaatcca
     9301 ctcatcattg gtatccataa tctgagccaa gtcgtgattt gtaaccactt gctctggcac
     9361 ataatgagca acctgactta tttttgcaaa agccattatt tcaaatcctc caaaaattgg
     9421 taaagattag tcaaaccttt acccatgaca gcaatttctt cctcgctcat gccatcaata
     9481 attttttcta ccatggcctt gtggaagcgt ttatgcagcc tatgaatcaa gcgacccttc
     9541 tttgtcaaat gcagatgcac cacacgacga tcctgttctg accgaactcg ctcaatgtag
     9601 cccttacgct ctaaattgtt caaacttgtc gtaacagttc caagagttac catcaactct
     9661 tttgacactt gacttggagt cacgtctgga gccttaccga tgacatcaat cgtatgcatt
     9721 tctttgatgg agatatcctt aaaacgacta cctctcaagt tcacttcctc aattacaagg
     9781 acattgttaa atatagatgt taaatattca ttgattcgtt ggtagtccat ttttcatatc
     9841 cctccttctt caaaaaactt ttacaatcaa aacatttgac agaaagaatt atatcaaaat
     9901 tcaaagaatg atgcaagtat tttttatttt cctgtaaatt taggtcttct tctctccgaa
     9961 tgagctcgca ctccttcttt gaaatcctct gtttgagcta aggatttctg taggttcagt
    10021 tctaaagtag cgtaaccctg ccagtcttta aattggctct cccaaaccaa cttcttaatg
    10081 gcagcatagg aattacttga aacacgtctt aattttttaa gaagctgttc tctcgtcttt
    10141 tcaagttttt cagcttcaga aacgcggtaa accaaccccc attctagggc tttttctgcc
    10201 gttaaagcct ctcctgtcat agctaattga gcagcacgcg tcacaccgat actgcggctc
    10261 aagagatgaa ttccccctgc atctggagcc aaaccaacac caacaaaggc ttggataaac
    10321 ttagccttat ccgtcgccaa acagaaatct gccgcaacag ccatattcgc tgcggcacct
    10381 gcaacagcac cgtcaacttc cattaaaaca ggtttggcta tttgcttgat tttataagaa
    10441 atcgtattga ccaactctgc gatttttgtc aatgatggaa tatcatcctc atccactgcc
    10501 cgcttcatct ctaccaaatc tcccccaact gagaagacct ttccattggc attgattaag
    10561 ataaaatgca cagctggatt ttcttctgcc aaagtcagag cttctaaaat ctcctcacac
    10621 atgggaatat gaaaaccatt tgcgacctca ggacggttca aggtaaggat tgccaagtcc
    10681 tcttcaagct gataaataat gtgttccatc agggctcctt tttattttat aagcagttta
    10741 tactcttcga aaatctcttc aaaccgcgtc agcttccatc tgcaacctca aagcagtgct
    10801 ttgagctgac ttcgtcagtc ttatttacaa cctcaaagca gtgctttgag cagtctgcgg
    10861 ctagcttcct agtttgctct ttgatttcat tgagtgttaa attatttcac tgtcaaagta
    10921 tatctttttt aggagcagaa agcaagaaaa aatcgactga aagtgtctca gccgataaga
    10981 aaggctcgct ttaggctaga aagcatctta ttttcttcat tgaaaaaagc ctttctttct
    11041 gctataatcg tataaaatac ttactttagg agttcttatg aaagttgtta aatttggagg
    11101 tagttctctt gcctctgcta gtcaattaga aaaagtttta aacatcgtca aaagcgattc
    11161 agagcgtcgt tttgtagtcg tttctgcgcc tggtaaacgc aatgctgaag atactaaggt
    11221 tacggatgcc ctgattaaat actaccgcga ctatgttgcg ggtaacgata ttagcaagag
    11281 ccaaagctgg attatcgacc gctatgctgc tatggttagt gaattgggac taaaaccagc
    11341 tgtgctagaa aaaatttcta aaagcattca cgccttggcc actcttccta ttgaagaaaa
    11401 tgaatttctc tacgatactt tcctagcagc cggtgaaaat aacaatgcca aattgattgc
    11461 tgcctacttt aaccaaaatg gtatcgatgc acgctatatg caccctagag aagctgggat
    11521 tgtggtcaca agtgaacctg gtcacgctcg catcattcca tcaagttatg acaagattga
    11581 agaattgaca aacaccaatg aagtccttgt cattcctggt ttctttggtg tcactaagga
    11641 aaatcaaatc tgtactttct cacgtggagg atctgatatt acaggttcta tcattgctgc
    11701 tggtgtcaaa gctgacctct atgaaaactt tacggacgtt gatggtatct ttgcagccca
    11761 ccctggtatt atccaccaac cacactcgat tcctgagttg acctaccgtg aaatgcgcga
    11821 gttggcctat gcaggcttct cagtccttca tgacgaggct cttcttcctg cctaccgtgg
    11881 aaaaattcct ctggttatca agaataccaa caaccctgac catccaggta ctcgtatcgt
    11941 tctaaaacac agtaatgatg aatttccagt tgtgggaatt gctggtgact caggctttgt
    12001 cagcattaat atgtcgaaat acctcatgaa ccgtgagatt ggatttggcc gcaaggttct
    12061 gcaaatcctg gaagaactta acatcggttg ggaacatatg ccaacaggta tcgacgatct
    12121 ttctatcatt ctccgttctc gccaactaac tcccatcaag gaagaagaaa tcctgcgtca
    12181 gttggttcaa aaggctaaag tagatcatgc agaaatcgaa cacgaccttt ctatcattat
    12241 gattgttggt gaaaaaatga agagccatat cggagtgact gctactgcga cacgcgctct
    12301 atctgaaaac aaaatcaaca tccagatgat gtctcaaggt tctagtgaag tttctattat
    12361 gtttgttgtc aataaagacc aagagaaagc agcaattaaa gccctctaca atgccttttt
    12421 tggagaaagt aaggaagact aagcatggcc caatcactta acaaaacagt actcctcagt
    12481 acgacaggca cttcctacct ctctatcgct gggaaagttg ggaaattcct tgtcggagat
    12541 caggctttgg aattttaccc agatgtcaat gtcgaacaat ttatccagat tccttggagc
    12601 catatcaatc agattggagc caatgtcact ggacgcaaaa tcagccgcca cttcgaagtc
    12661 tttacagaca aaggaaaatt cctctttgct tcaaaagact caggtgccat tctcaaaatt
    12721 gcacgcgaaa aactaggcaa tgacaaggtc gtcaaacttc cgaccctgat tcagacaatt
    12781 agccaaaaat ttaaaaatct atttgcaaaa aagtagaaac tttagtataa tcatagcaac
    12841 ggataagtag gttatcttct tctttcagaa agtctgcggg tgctgcgagc agataggagg
    12901 gataggtgaa attctaccgt tagtgacaat tacatacaca atcaagtgca cacctgtgaa
    12961 gttggatgga accgtggccc tgccactcca acgttttgtc agggtgcttt tttcataaag
    13021 gagttcttat gttagatatc aaacgtattc gtacagattt tgaagctgtc gcagaaaaat
    13081 tagctacacg tggtgtagat gctgctgtct tgaatgaaat gaaagaaatc gatgctaaac
    13141 gtcgtaacat cttggtcaag gttgaaactc tcaaagcaga acgtaacaca gtttctgctg
    13201 agattgccca agctaagcgc aacaaggaaa atacagatga caagattgct gccatgcaaa
    13261 atctatctgc tgaggttaaa gccttggatg ctgaattggc agaaatcgat gctaaattga
    13321 cagaatttac aacgactctt ccaaatatcc cagctgacag cgttcctgtt ggggctgacg
    13381 aagacgacaa tgtggaagtt cgccgttggg gtactccacg cgagtttgac ttcgaaccta
    13441 aagctcactg ggatctcggt gaagaccttg gtatccttga ctgggaacgc ggtggtaagg
    13501 taacaggcgc tcgcttcctc ttctataaag gcctcggtgc tcgtttggaa cgtgctatct
    13561 acaactttat gttggatgaa catggaaaag aaggctatac tgaagtcatc acaccttaca
    13621 tagtcaacca tgattctatg tttggtactg gtcagtatcc aaaatttaag gaagatactt
    13681 ttgaactcag cgataccaac tttgtcttga ttccaactgc tgaagttcct ctgacaaact
    13741 actaccgtga tgaaatctta gacggcaaag atcttccaat ctacttcact gctatgagtc
    13801 cgtcattccg ttctgaggct ggttctgccg gtcgtgatac gcgtggcttg atccgtttgc
    13861 accaattcca caaggttgaa atggtcaaat ttgccaaacc agaagaatct tacgaagaat
    13921 tggaaaaaat gacagccaac gctgaaaaca ttcttcaaaa actcaacctt ccataccgtg
    13981 tcgttgctct ctctactgga gatatgggct tctcagctac gaagacttac gacttggaag
    14041 tgtggattcc agcacaaaac aattaccgtg aaatctcaag ctgttcaaac acagaagatt
    14101 tccaagcccg tcgtgcccaa atccgttacc gtgatgaagc agatggcaag gtgaaactcc
    14161 ttcatacctt gaacggttct ggacttgcag ttggacgtac agtggctgca attcttgaaa
    14221 attaccaaaa tgaagatggt tctgtgacca tcccagaagc acttcgtcca tacatgggtg
    14281 gagctgaagt catcaaacca taaaaaataa ggtttagcta tttctagcta gacctttttt
    14341 cgtaaccaaa tcagataagc acctagtaca cagaataaaa tagttaggca tataatggtt
    14401 tcagccaata ccaggtaatc cagaaatgga agtttcaaaa ttccctgagc catcttgagc
    14461 gaggtcgctg tgataatggt tgggaaggtg agggctgaga aggctggttg aaaaccttgt
    14521 tttaaaatgt tgggcagacg agttaaaaca aagaaaaaga aggattgaga agccaaaatc
    14581 atgacaatca agacccaagt cggcaggctg gttcctccta ctcgaactag agaagccaag
    14641 agtagagaga aaggagcaca gtagattcct tcttgtccaa gcaaggctag tgggagtgga
    14701 tgtttcttta aatcgctata aataagggga tagagataga aggtcaagag aaaaccaaaa
    14761 ctcaaggtcg cataggcaat ttcgataata cctaccagag gataggtcaa ggcagccact
    14821 gctatcccca catagagaac cgtccaactt ggagtggcat gaaccctccg ccctggacaa
    14881 gcaaacttga tggtaaaacc agcaatcaag gtcaaatcca agagaaatga aaaccaccaa
    14941 atcccttgtg ctaccaaagg aagataagag aatacgcgaa agacataggt cgataaaatc
    15001 atcccagcca taggaaaggt tgccattcct gacaaaagag ggggcttggt caattcttgc
    15061 ttggtttctt tccaattaaa gagatgcaga attagaaagt aaatccataa aaccaaacca
    15121 gtcagactaa aaagatggga tagaaccggc aacgtatcta aaataagatt tccagctcct
    15181 gccaaaccta gcaaacaacc tgaaaatact aaggggagtt ttttcatcct aacctccaat
    15241 aatcatgtta gtttcagtat aacataaaag cgcttaaatg aggatttaaa aaaacgaagt
    15301 ccgcttattt cagacttcat tttactcaga tatgaattag gcataaggtt gcaattctgg
    15361 attaattggt gtattagcta agttgttggc atagttacag aggattgcta ggctgacacc
    15421 aaaaaccaca tccaaggcat tttgttgagt gtagccagct tctaaaaact cagacaaggc
    15481 ttcatctcct acacgaccct tggtattgat aactgccaag gtaaacttag ctagggtatc
    15541 caatttagga tctgtttcaa ttggagtacg attgcgaaga gcttgaatca agtcatcatt
    15601 catctggatt tgtttgatgg aaaaggctgt gtgacctgcg acacagaagg cacaaccatt
    15661 ggtcacggct gccgtgattt gcaccacttc acgctcaacg ggtgtcaggc tgttgcgacg
    15721 gtggatagat gagacaattt ggtaggcttc taaaacagtc ggggcattgg ccaagagacc
    15781 gattaggttg ggaatatagc cattgttgtc tttttctact gtttcaagaa tttctttcac
    15841 ttctgctggt gctgactcta ctgtatggat agtaaatgtt gtcataagat acctcttttc
    15901 ttattattga cactaatatt attggaaaat cttataaaat cctgattcct aagtttatct
    15961 aagataaagc tttattctct cataagattt tcgttgttat attagtttat cacacttcca
    16021 atcacttgca taatatatat tatatatcag gctgataaaa attatttata ggcaaaaaaa
    16081 tcacacgagc tgtgtgattc cattatttgt caaaatactt tttagtttca gcaataacga
    16141 ctggcgacaa gaccaagagg gcaatcaagt ttggcagaac catcaaggca ttaacgatat
    16201 ctgcgataat ccagaccata tccaactcga taaatcctcc taacaagacc atgagcacaa
    16261 aaaccacacg gtagagccag ataaagcgaa ccccaaagag gaactcaaaa cagcgttctc
    16321 cgtaatagtt ccaacctaga atcgttgtaa aggcaaaaag tacaaggaag atggtcaaga
    16381 gggcaggccc aaagtgtgaa aagactgttg agaaagctga ctgagtcaag gcaaccccat
    16441 tcaagtcacc actccaaact ccagttacca agatggtcaa accagttaga gtacaaatga
    16501 tgagggtatc aataaaggtt cctgtcatgg aaatcaaacc ttgctctact ggttcatttg
    16561 tcttggcagc tgcagctgca ataggagcag aacccagacc agattcgttt gagaacacac
    16621 cacgcgccac accattttga atagccatcc gaacgctagc accagcaaat ccacctaccg
    16681 cagcaagggg actaaaagct gaggtaaaga ctaaagcgat tgtgccaggg atttttccga
    16741 tattaaagaa aataactgta agagttccta agatataaat gatggccata aaaggaacaa
    16801 cagtagttga aaccttagaa atagacttga gtccaccaaa gactgcaatc gctacaaaga
    16861 cagacaagac gagagctgtg atggctggcg aaatcgtcgt tgtattttgg atagattctg
    16921 caatcgagtt gacttgggtg aaggttccga ttcccaagag agcaaccaat actcctgcta
    16981 ctgcaaacaa aacagcaagt ggtcgccact tttctcccat ccctagaagg atatagtgca
    17041 tgggacctcc cgctactgca ccatggtcgt ccttggtgcg gtatttgatg gccaagagtc
    17101 cttccgcata cttggtagcc attccaaaga aagccgccat ccacatccaa aatagagctc
    17161 ctggtccacc aaccttgata gccgtcgcaa ctcctatgat atttcctgtt ccaacagttg
    17221 atgccaaggc tgtacacaga gctgcaaaac tggatacatc accatgtccc ttatcctgga
    17281 taaaaataag ctgaaaggcc ttgggcagac gcaaaacctg caagagtcct agccgcatgg
    17341 ttaggtaaat ccctgttccg accaataaaa tcaagagggg cggtccccaa gcaaaagcat
    17401 cgattgattt aagcaattct aacattttct tctcctatcg tttcaacccc aaaagaaaga
    17461 gcacatgcaa gatacatgta ctctggaatg cttagataaa tgctaaaaag cggtctatcc
    17521 tagctctgtc cttttacctg agagtttgag cagttgcctg ccttgcccct tcggtgcctt
    17581 tacggtctct ccagagttcc gtccatttac agtcatggaa aatcaaacga ttccccactt
    17641 ctattaaact tcattcggtg ttggtattta attgattcta attttacaaa aaatgttggc
    17701 ttttgtcaat gtgtttatta gtaaaaatta gttcaacagt ttttacttta taaagtccag
    17761 aatactgcta tcctttaaaa gtgacaatag tcgcaccact gcctccagca ttttgtgggg
    17821 catagccgaa actcttgaca tgtttgtttc tttgcaagta tttggtaact ccttcacgga
    17881 tgactcctgt tccgatacca tggatgatat caacttgagc catattgtta agcaaggctt
    17941 ggtcgatgaa ggtatctagc tcattcatgg cttcttcata gcgcttgcct cgaagatcca
    18001 gtctagcttg aggtcctcgc ccagaagttc gtttcacaac attgacctgt ttcttcttga
    18061 ctggtttttc ttgctgggct tgaacaagat caaactcttt ctcttccaag gtcatcttaa
    18121 tcaagccaac ttgggcttcc cagcgaccgt ccttgagttg actggtcaag gtaccacgct
    18181 gaccataact gagaaccacg atatcatctc ccacctttgg agctcgtttt ttcttggcct
    18241 tttgaaggac cttattttta gacaagtcca ctttttcagg agccaatttt ttcaacttgg
    18301 ccttggcttc aatgatttcg tggggcttga gttgggattt actgtggaga tttttgagaa
    18361 tctggtcact ttcacttagg gccatatcca caatctcagc agcctgttca cgcgccttgt
    18421 taagctcggt ttccttttca cgattaagct cgttgtagag tttttttagc gcacggttca
    18481 tcttgagatt ttcttgctcc acctcacgga tattgtccaa gcgtttgcgg ctttccagcg
    18541 tctgctcttc taattgctca atgatacgat tgacgtcatt gtcctgatcg atctgctgac
    18601 tggcatctcc tacgataact tcagataggc ctagacgttt ggcaatttca aaggcattac
    18661 ttcggccagg aacaccctgc ataaagcgat aggtcgggcg aagagttgca gtatcaaact
    18721 ccatactggc attttgcaca aaggctgtct caataccgta ggccttgagt tctggatagt
    18781 gggtcgtcgc catggtcttg atttgacgca ggcgaaggtc ctccagaata gccatggcaa
    18841 gggctgctcc ctcttgggga tcagtaccag cccccaactc atccaaaagt aagagtgaat
    18901 gttggttgac cttgccaaga atatccacga tattggtcat atggctagag aaggtagaca
    18961 agctctgctc aatagactgc tcatctccaa tatcagcaaa gatttcttca aaaataccaa
    19021 cacgacttcc cttgtctgct aaaatcggca atcctgactg ggccatgacc tgtgtcaagc
    19081 ccagagtttt gagcatgatg gtcttcccac ctgtattggg acctgtaatg acaatagctg
    19141 ttaaatcttg accaaaatag acatcatttg cgacggcatt tttgaccaaa ggatggcaga
    19201 catggagcag ttgaatctct tgattttctg acagctgagg cacgactgct tgtctttctt
    19261 ggataaatcg aaccttggca cgaatcaagt ccagatgacc gataatccaa gcgtcattag
    19321 caatctcagc cgcatgaggg cggacacgct cagaaatttc ttggagaatg cgaagcattt
    19381 catagcgctc atctgctcgc agactagcaa tttcttcgct cagtttgact acctcacgtg
    19441 gttcgatata gacggtgttt ccactagcag aaatatcatg aacgacacct gcaatcttat
    19501 tgcggtaggt gtttttgact ggtaaaacct gacggccatt tctgctagca acaattcctt
    19561 ccgtcaacat ctgcgctttt tgcttgagca agtcttgtaa aacatcgcgt acctgactct
    19621 cgctatcatg tatttttcga cggattcgcg ccaattcttc actggcaaaa ttttcaatga
    19681 aacccgcatc attaaaggcc tgaagatttc cttgtaattg cggaaaatca tgtaatttct
    19741 caaaccaaag ggctaattct tccaagctga cattttccag attggtgtaa aaattttgaa
    19801 gttctcggct ggcaagaagc acgcgtttca agagtaggaa ctcctcgata ttgagatccg
    19861 ctcccatctc caacctcttg cagactcctg caatttcctt agttgagaga atagtaaaat
    19921 gcggttgctc gacgaaaaga gcctgcattt ccttcatctc agcaaaagcc tgtttgattt
    19981 tatctgcttt ggcagtcgga gccagttgtc tcaattgctc caagccctgc tcggtcaaca
    20041 aatgaggctc aaacaaggcc ttgaccttat cgaactctaa tgtttctaat attttcttat
    20101 tcatctttat ttccttgtct ttgaatgtct aggtgtggta gctttttaca ttctgataga
    20161 ttcgagtcaa atctgtaaac aaagggctag gaaaactcct gcccttttta tccgattaaa
    20221 tttgtcaccc agatttgttt gagccaactg gttgttatcg gtatgctctg gatgatgtgt
    20281 tttgcgacga tactcttttc aagaggattt tgtataactg ccatggggat ggtcgccaag
    20341 attgtcaagg ccatttgcaa gacaaataag gtcaccaaca tggacaagat acctgctgaa
    20401 acttggaaca acttaccacc cagttttttg ctaggaatca agtgtaagag aagaccaagc
    20461 aaacgaccaa tgctatagac aatcccaaat acaagcaagt agccgatacc tgcataaaag
    20521 accttatcca gctgaaagag ttgatccgat gggaaaaaga aagtcccctg accttcctgc
    20581 gaatttgcat aagggagcaa taaatggaat tgctctccaa gccccttata aaactggcca
    20641 gccataaaag ccgatgccat ggctgaaatc aggtaataaa cctgtaagag caggcctctc
    20701 cgatagccga tataaaatcc ccaaaccaag accaatagaa gaaggaatga aatcataagg
    20761 aatcctcaat cttgctctgt tcttgcttac aagtcacaag cttgtgacgg agttcttcta
    20821 gctcttgctc cttatcgtca aattcaatct cacggctgag ctgagttgat aaacagttga
    20881 ctgccaacaa aagagcgatt gtttcatcat ctgcgctagg catttgttct ttaattgctt
    20941 ggtatttttc tgtcgcaacc ttagcgattt cctccataaa aaggttatca tgttcgcttg
    21001 tcaaggttaa cgattttttc ccgaatgtaa atttgaatcg atttagattt gccataaaat
    21061 tcacctcacg atattatacc aaaattcgct aattttgtca gtttttacaa attttactgc
    21121 ttttgtggta caatagaaac tatggcaagt atcacactca caccaagcga aaaggatatt
    21181 caggcttttc ttgaacacta tcaaaccagt ctggctccca gtaagaatcc ctatatccgc
    21241 tactttttga aactacctca agcaacggtt tctatctata cttctggaaa aatcttgctt
    21301 cagggtgaag gggctgagaa atacgctagt ttctttggct atcaacttgt agaggaaaac
    21361 agggggcaaa atcttccttt gattgggaca gatgaggtag ggaatggttc ctactttggt
    21421 gggcttgcag ttgtggcttc ctttgtcaca cctgaccagc atgccttctt acgaaaactt
    21481 ggtgtgggag attctaagac tctgaccgac caaaagattc gtcagattgc tcctattctc
    21541 aaggaaaaaa tccagcacca ggcactcctt ctctcaccca gcaagtacaa cgaggtcatc
    21601 ggagaccgct acaatgctgt ttcggttaag gttgccctcc ataatcaggc tatctatctc
    21661 ctccttcaaa aaggtgttca gcctgagaaa attgtgattg atgcctttac cagtgctaaa
    21721 aattatgaca agtacttggc acaagaagcc aattgtttca gcaatcctat cagcttagaa
    21781 gaaaaggctg agggcaaata cttggctgtc gcagtttctt ctgtcattgc gcgtgatctc
    21841 tttctggaaa atcttgaaaa tttgggacga gaactgggtt atcagctccc aagtggagct
    21901 ggaacggctt ctgacaaggt ggctagccag attttgcaag cctatggtat gcagggactc
    21961 agcttctgcg ccaaattgca ctttaaaaac actgaaaaag cgaaaaaacg cttagaaagg
    22021 taagttatga attcatttaa aaatttctta aaagagtggg gattgttcct cctaattctg
    22081 tcattactag ctttaagtcg tatctttttt tggagcaatg ttcgcgtaga aggacattcc
    22141 atggatccga ccctagcgga tggcgaaatt ctcttcgttg taaaacacct tcctattgac
    22201 cgttttgata tcgtggttgc ccatgaggaa gatggcaata aggacatcgt caagcgcgtg
    22261 attggaatgc ctggcgacac cattcgttac gaaaatgata aactttacat caatgacaaa
    22321 gaaacggacg agccttatct agcagactat atcaaacgct tcaaggatga caaactccaa
    22381 agcacttact caggcaaggg ctttgaagga aataaaggaa ctttctttag aagtatcgct
    22441 caaaaagccc aagccttcac agttgatgtc aactacaaca ccaactttag ctttactgtt
    22501 ccagaaggag aataccttct cctcggagat gaccgcttgg tttcgagcga cagccgccac
    22561 gtaggtacct tcaaagcaaa agatatcaca ggggaagcta aattccgctt ctggccaatc
    22621 acccgtatcg gaacatttta agagacctaa gaggccgaga atcaccaatc tcagcctctt
    22681 cttctatcgt gagaagtgat tgtttattga caaatactca atgaaaatca aagaacaaac
    22741 taggaagcta gccgtaggct gcttaaaaca gtgttttgag gttgtggata gaactgacga
    22801 agtcagctca aaatactgtt ttgaggttgc agatggaagc tgacgtggtt tgaagatatt
    22861 ttcgaagagt ataaaataaa aacaatccca actctcacct attcatgcaa aggaatttta
    22921 tggaagttta tttttcagga accattgaac ggattatttt tgaaaatccc agcaattttt
    22981 atcgcatcct cctcctagaa atcgacgata cggacgcaga ggattttgat gactttgaaa
    23041 tcattgtcac aggaaccatg gctgatgtaa ttgagggcga agactatact ttttgggggc
    23101 aaattgtcca gcactccaag tatggagaac aactgcaaat cagtcgttat gatcgcgcaa
    23161 aaccaactag taagggcttg gtcaagtact tttcaagtag ccatttcaag gggattggtc
    23221 tcaagacagc tcagaaaatc gtggatacct atggcgaaaa taccattgac gaaattttgc
    23281 aacacccaga aaagttagaa ggcatcgcag gactctctgc caaaaatcgc gaggctttcg
    23341 tctccactct ccgtctcaac tacggaacgg agatggtttt ggccaaacta gccaactacg
    23401 gcattcccaa caaactagcc tttcagattc aagactttta caaggaagaa acccttgatg
    23461 tggttgaaaa ttatccctac cagctggttg aggatatcaa gggtttgggc tttaccattg
    23521 ctgaccaact agctgaggaa ctaggcatcg aaagtcaggc tcctgaacgc ttccgcgccg
    23581 gtctagttca cagtcttttt caggcctgta tggaaacagg ggacacctat gttgaagcac
    23641 gggatttgct ggaacaaacc cttactctcc ttgagtcttc ccgtcccgta gaactagacc
    23701 ccagccaagt tgcccaagag ctctcctacc tgatcgaaga agacaaggtt cagcagattg
    23761 ataccaagat ctttgacaac agcctctttt tcgctgagga aggcatccgc agtcacttgg
    23821 ttcatatcct tgaaaaagga aaacagaaga gtcatgattt agaaaccatt caaaaacata
    23881 tcactactat cgaggaagag ctgggaattg agtatgataa cattcaaaaa caggctatct
    23941 gtgatgctat ccagaataag gtctttatcc tgacaggtgg acctggtact ggtaagacaa
    24001 ctgttatcaa tgggatcatc gctgtttacg ccctgttaga gggacttgac ctcaggaaga
    24061 aaagcaatct gcccattctt cttgctgctc caactggacg agccgctcgc cgcatgaatg
    24121 aattgacagg tttgcctagc gcgaccatac accgccactt gggaatgaca ggtgacgatg
    24181 ataccagtca tctggaagat tacctagatg ctgactttat catcgtggat gaattctcta
    24241 tggtggatac ttggctggcc aatcaactct tctccaatat ctcttctaac agtaagatcc
    24301 tcatcgtagg tgacagtgat cagctacctt ctgtcagtcc tggacaggtt ctagcggatc
    24361 tgcttcatat tcctttgatt cctcagactc gcttggaaaa aatttatcga caaagcgaag
    24421 aatcaaccat cgtcacccta gctagtcaga ttcgacaggg catcttgcca gctgatttca
    24481 cccaaaaaaa agctgaccgt tcctactttg aaattgctag tggccatatt cctgccacca
    24541 ttgaaaaaat cttaggtgcc gccctcagaa atggtattcc tgcccgtgat atccaagttc
    24601 tggctcctat gtaccgaggg acggcaggga ttgatgctat caatcagctc atgcaagacc
    24661 tgctcaatcc cccacaaaaa gatcaactca gttttgaagc tcctcagtgc cactatcgta
    24721 agggagacaa ggtcattcat ttggttaacg atgctgaaat caatgtcttt aatggggatt
    24781 taggagctat cacagacctg attcctggta aatacaccga atcgaaacaa gacgagattg
    24841 tcattgattt tgatggtaat gaagtctctt acccccgtaa cgaatggtac aagattcgct
    24901 tggcctatgc catgagtatt cataagtctc agggaagtga gttcccagtt gtcatcctac
    24961 cgattactag tgctagccga cgtatgctgg agcgaaatct catctacaca gccattacac
    25021 gtgccaaaag caaactcatc ttactaggcg aattacaagc cttcgactat gctacccaac
    25081 acatcggaac tgcccgaaaa acctatctga ttgaacgctt cagtgatcta ctggagaatg
    25141 ttgaagaaaa gcaacaagct gtctctgaaa cagtcacatc aagtgcctct gaacaatcct
    25201 acatcctaac cgaagaaaac tgggacagca ttccagccat gattgggatt acagacacag
    25261 acctcaaaga gatttttgga aaatagtaca tcagaaaacc caccaattca ggtgggtttt
    25321 ctgtttatta agattatttt actgttgctg tgcttgtaat caattcaaca gcttttttga
    25381 ttgtgatatc atgtttcaac atgtcagctg aaagcaagct ttgtacttga gcaacttcca
    25441 tgttgtagtc tgctgccaat tgctcaactt ctttttggat ttcttcttct gaagcatcaa
    25501 atccttcagc tttggcaact gcttcgataa caaggttagt cttagtacgt gactcagctt
    25561 ctgcttggta ttggttgtga aggtcttctt gagtagttcc agtgatttgg aagtacatgt
    25621 cagggttgat cccttgacgt tgcaagttcc caaggaattc atttactgaa cggtgaactt
    25681 cttcatggat catttcttct ggaagttcta cgatttcagc gttttctaca gctgtatcaa
    25741 ttgctgcacc ttcaactgca tctttgtaag cttcttcttt agcagcagcc aattctttgc
    25801 ggtatttttc tttcaagtca gcaagtgttt caacttcttc atcaatgtct tttgcaagtt
    25861 catcgtcaag agctggaact tctttagctt ttacttcgtg gatagttgtc acgaatttag
    25921 cttctttacc tgcaaggtct tctgcttggt agtcttctgg gaatgttacg ataacatcaa
    25981 cggtttcgcc agctgagtga cctaccaatt ggtcttcgaa accagggatg aattgacctg
    26041 aaccaagtcc aagtgagaag ttttcacctt ttccaccgtc aaattcaaca ccgtcgatag
    26101 aaccaacgaa gtcgatcaca acagtgtcgc cgttttcagc agcagcttcc ttgataacca
    26161 attcagccag gttgttgcgt tcgcgttcga tacgctcttc aacatcagcg tcagttactt
    26221 ctttttctac atcaactgat acttcaaggt ttttgtagtc acccaatttt acttcaggtt
    26281 ttgtaacgac ttcagcagcg ataacccagt cttgaccttt ttccattgaa gttacgtcaa
    26341 tttttggttg ggcaaccact tcaagaccag cttcttttac agctgcttca taagcgtttg
    26401 gcaaaagagc gttcattgca tcttgataaa gagcttcttc accaaatttt tggtcgaaga
    26461 tagggcgtgg aaggtgacct ttacggaaac ctggaacatt aagagatttc ttcactgact
    26521 tgaagacacg gtccaattct ggtttgattt ggtcttgaga gatagtgaaa gtcaagacac
    26581 cacggtttgt ttctttgttt tcaaatgata cagacattct gtcatttctc cttaaaattt
    26641 tttaaataca gtctattata acattttcta taaataaaat ctagactaat gcagccaatt
    26701 atagacaaca agcctttttc ctaactaaaa cactcataaa gtatagacaa ttttagacta
    26761 ctttggatat cttttggtta gtcattttcc aaaactaatt tatctaattt ttcagttata
    26821 tagtagattg aaactagaat agtacacctc tacttctaaa acatttttag aaatcgattt
    26881 gactgtcccg atcgatttgt cctgttcttg tttcatttta ctatatagat cgcattcgaa
    26941 agaattcgtt ccaaaattgc ttggttcgtt cttctcttgg acgactaaag aattgatggg
    27001 cattattttt ttcaacaatt ttcccactgt ccataaaaat aacatgatgg gctacatctc
    27061 tagcaaaacc tatctcataa agctgatcaa aaactcaatc tgaggagaaa atccaataag
    27121 gctttccata ataaaggaca tcctatcaag atttttgaac acccgatagg ctgccttttt
    27181 ccgattttga agactttttg tccagcttct tttaccttat aaataacaac aaaaatcaaa
    27241 gacaagctag aaagattacc gcagataact caaacagtgt tttgaggttg cagataagac
    27301 tgacggagtc agctcaaagc actgctttga ggttgtagat agaactgacg aagtcagtaa
    27361 catatgccta agacaaagcg atgctaacct gatttgaaga gattttcgaa aaagattaaa
    27421 tatactctac aatttgctca atcaattctt ggtcgtctaa acctgtaact tgtataacaa
    27481 catctttgac tgattgttta gccaacaatt cacctaatcg aatactttct tcatcattta
    27541 catcgcgata gtcaaaggca tagccaactg ttttaagtag gtttttatat gacaaactga
    27601 gttctttcaa ttcacgtatc ggccggatga atcgttcatt atagcctaat tttcggattg
    27661 gagtgcgagc tacgcgacta acctcgtccg ctatgaaagg gttttcaaac cgttctataa
    27721 tgactttgtg ataattctcc aattcttttt tatcaaagtt ccatttggca atcaagagac
    27781 tccgaatttc agctaataca gattcaatcc gagatttgat attaggattt tgaagagctt
    27841 ccaaaattgt cttggcacca taatgcgcac caatgtaagc tgaagttgca tgtccagaat
    27901 tgactgaaaa aagttttcgc tcaataaagg gttctaaatc ttcttcataa tgcacatctt
    27961 ttagacgtaa atctggattt ttaagacgct tggtttccac gacccattca ttaaagggct
    28021 cgaccacaac aaaaagggaa tcttcgtgac tttgtgctgg aacaatcctg tctactgcag
    28081 catttggaaa acctatgtag ttatcagcaa atgtcaaacc ttccggactt aaatatttct
    28141 tgacttcttg ataaagaaat tgagacccgc caatcatatt ttcacaggcc ataacatcca
    28201 atgcctgtgt atttcctgca actcggcgag cttcgattcc tttggctaga agttcggcga
    28261 taaaagggag tatattaggt ccgattgcag tagtaataat atccgtcttt tgaatcgctt
    28321 caatgacttg ctcaggatgt tctttgctat taatgccagc cacattagtt acttctatac
    28381 gagactgtcc tttctgtgca atttcaattt catacttgcc cttttcattt agagcatgaa
    28441 ttatctgatt attgacatcc acaaaatcaa tatggaaacc atttttaaat agaatttcac
    28501 ctataaaacc acgaccgata ttaccggcac caaaatgaac agaatgtttc attcaatttc
    28561 tccttttcct atttgaggga catatatact agatgacaaa tgacgaaatt caattattca
    28621 acagcatcaa ataagcgcaa tacttcctct tttgactgag catctgctag tttaagaaca
    28681 ttatctacat ctgcacagaa gatagaaatt ttctgaataa tttctaagtg ttcattacca
    28741 ataccagcaa taccaaaaag aaccgttgcc acttgagggt tagatacatt cccaaaatca
    28801 acccctatag ggacttgaac gactgtaata ccagacttga ggacatcctt ttttgctgca
    28861 tctgttccat gcggtatggc gataaagtta cccatgtaaa cagatagctc tttatctcgc
    28921 tcaatcatgg cttcaatata gtcttcatta acatatccac cctcataaag aacttgccca
    28981 caataacaaa tagcttcctc cttgttagaa aattgtttat taagcttaat caaatgtttt
    29041 tcaagtttca tattagttct ccaatttctt aattttttcg ttaaaaatag tatttagcat
    29101 ctgataaata atggattgat ttcccgtctt gtaaatctct gtataaagat gattttcaat
    29161 aatcgactga ctaatagctg tcattaaatc tctcgcttcc tcgctagctt ctttagacat
    29221 tagcattagc agacaccttt tgacctcttc ttcctctcgt ttcattgaca atgcggagat
    29281 aggtagtttg agttcaaaca tagtaaaact atttgttgtt actttgctag actgactatg
    29341 taaaagcacc agaccagtat taggaatagc cataggacta ttctggaagt gagataacaa
    29401 ttttcttgtt aggtaatctc tgtcactcac attcttctga tattggatga tttgttctac
    29461 cgtgtgctca aatgattgat tattctccaa ttgaaccaac tcgaattgac tcaaaagctg
    29521 actactagat attaaataat cttgcaagtc ataacttttc tctggagcga tagcatcacg
    29581 cgcgacaatt gcacgatgtg cctgtacata ctgaagtcgt tcctgtaatt caagcaattc
    29641 tttgacggtt ggaaaactag aaattacatc gatttcctga ttagtcagcg gcttggtaga
    29701 taaaatataa tcatactgac tgagatcaat actgtggtaa tctactagag actgaatatc
    29761 tatcaactct acaaaaggag caacactctt aattttactg actaataaat ctctagtgac
    29821 ccgacgttca tccgttaaaa gcaaaacacg gactggataa atctctggac tacggcctaa
    29881 gctagagata aaatgtaggg caatcatgcc atactcatac tctgtatgaa gatatttcgg
    29941 aaaaatatca ttcactaaaa gactgatgac tgtataaaga aatttattcc tttcaactaa
    30001 aatctggaca gattctggca aattttcacc ctgaaaaagg atagggacgc ctaaactgag
    30061 ccgaatatga tggaaaagaa aattgaataa aaccttgtcc ttaaaaaagt caatcttggt
    30121 atacatggaa accgtatcaa tcagatttga aatattgtag aaaaattcac catcaaattt
    30181 ctccgtaaag agcggattgt cctgacgttt aatgattaat tcatccaaga tgctcgcaaa
    30241 atagataatt tcctgaatac tataaaattg tgcagtctgc ttagagtaag cttgaaaaat
    30301 tttttgagaa atttccaaag cctgcttact agtattaggt gaattttcaa tgttttgctc
    30361 ctgacctata agagataaca agatcgcaaa aaacatcttc atcctagcat ccatatctgg
    30421 aaaacctgac agttgcttat taacaatctg actggccagc ctagttctat ctgcttctaa
    30481 aatatcaaag ctcccaaaat taccggttga aaaatctgct actgagatac agtttgtcag
    30541 taaaatagcc aaaaatcttc tcttaccaac tgaatcacca gaaatccgat aacctttttg
    30601 tcgttcaatt ttcagatcaa agtctaaaag acgcttatca atatctgaaa tatcctgaat
    30661 aatagttaca ttactgactt tcgtgcactc ttgcaatgct tcattggtga caaaaccact
    30721 ctcagtcaga aggcgatagg taatcaactc ttgtctttct tggggactat actcaactaa
    30781 cacttcaagt tctgtcggca aatcatccaa ctctccagtc aaaatatagt atttcccatg
    30841 cttcgttatt tcgattccat attgctccat gctatctgtc aaatctgata aagttcgata
    30901 aattgttcta gatgaaacct gtaagatttc agtcatatct tgcattgaaa gcttccctac
    30961 atgtaggaaa gccttcaata attgttcttc tcgtttggtt aatagcattc gtaatttaga
    31021 aacctctagt tctatttgta cattctagca gccattttgt catattctgg tgttgttact
    31081 aaactatcca caataagaaa ttgagaattc ggtgctgctt gctgcacttc tgcctgtaaa
    31141 gaaatagttg ttacaatcat tatgttttta gaattaaatt cacctaattc tgaaattttg
    31201 gcagtagaaa ctggaatacg aatattcttg tttctaaaga tagcccgaat cgtttcacag
    31261 cccatagttg cagttccctg tgctttacca taagcaacta ctacagcatc aatatggtta
    31321 aggtcaattt cctgactatc tactggtgct gaagttggta tatctccttc aatttctgct
    31381 attggagaag ctcctgttaa tgaagctaca atttcatcat aacgagggga ccctaagaaa
    31441 ttatcaacag aaacatgaat agcacttgga ctcttgtctt tagctcttgg tgtcagttct
    31501 tcctgagtaa caattaatgt ttttggtgta tcaagcaaat ttgagattgc ctgattagat
    31561 actggaatct ctagacctgc ttttttaacc ttatctcgaa gaatactagc tcccatagca
    31621 gagcttccca taccagcatc gcaggcgaaa atgatttttt ccactgagtc tgtcgaaaca
    31681 actgcatcaa cagaagttga tactaactga cctttagact gagccttagc tgcttgggta
    31741 gccgcctgag cagcttcgag cgaatcctca gttgacttgt ctgcatgaag aataagggct
    31801 gctacaagga aagaaacaac tgctgccact aaaacaccta aaagaacatt tagatggggc
    31861 caaacacctt ttggcgccgt agccataatc gcaataatag aacctggtga agctggagat
    31921 ttaagaccag cgtctaagag ttgaaaagta aaagttccag agatacctcc tgccatagca
    31981 gctaaaaata gagtaggctt catcataaca taaggaaagt aaatttcatg aatccctccg
    32041 aagaaatgaa taaccattgc cccccaagaa gaagatttag cagaaccttt accgaataca
    32101 gcataagcta atagaattcc caggcctggt ccaggattag cttccaatag gaagagaatt
    32161 gacttaccag cttgagctac ctgttctact cccagaggag taaaaatgcc atgattgagg
    32221 gcattattga ggaaaaggac tttagccggt tcgatgataa tattagccat aggaaggagg
    32281 cgagcattga caatagcctc aacaccattc ccaacagctc cagtaagagt cgatacgact
    32341 ggaccgattg cgtagaaagc caaaagcaat aatgcaaaac caacgagacc agctgagaag
    32401 ttattaacta acatttcaaa tccgggacga attttttctt ggaacttctc atcaaatttc
    32461 ttgatagtcc atcctcccag tgggcccatt accatagctc cgataaacat aggaacacta
    32521 gaacctgtaa ttgcaccaac agtagcaata gctcctacaa cggcaccacg ttggccatgg
    32581 atcatatatc cacctgtgta accaatcagg attggcaata aatacgttaa cataggacca
    32641 acaacagtag ctaactgttc atttggcaga tagccatcag cgataaagag ggcagtcaat
    32701 actccccaag caataaatgc tccaatattg ggcataacca tatttgaaag cgatgtccct
    32761 agtttttgaa ccctgacttt caatgatact ttttcttcca ttagatcatc ctccttttct
    32821 taaaactagt atatacaaag ttaaaaacct ttaaaactaa agttttgtca cagattttgt
    32881 gtcaaaacat tacttttgac gaggctgggc aaaaaggctt aaaaattata aaacgcatca
    32941 tatcaggtgt acatgaactg caccccaaaa gttagacaga aaaaatctaa cttttggggt
    33001 gtttttatta tgaaattaac ttatgatgat a
//
LOCUS       DBEROU010000024        32990 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.024, whole genome shotgun sequence.
ACCESSION   DBEROU010000024 DBEROU010000000
VERSION     DBEROU010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 32990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32990
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.024"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(372..521)
                     /locus_tag="ACL8CL_001178"
     CDS             complement(372..521)
                     /locus_tag="ACL8CL_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope"
                     /protein_id="HGS1330430.1"
                     /translation="MEKFNFKNNIGQENKLLQIEIYKFTNFCKLQNYTSVNIFSKDIF
                     EAIVN"
     gene            486..605
                     /locus_tag="ACL8CL_001179"
     CDS             486..605
                     /locus_tag="ACL8CL_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HGS1330431.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            complement(701..2047)
                     /gene="glnA"
                     /locus_tag="ACL8CL_001180"
     CDS             complement(701..2047)
                     /gene="glnA"
                     /locus_tag="ACL8CL_001180"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HGS1330432.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTVIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYIAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(2084..2440)
                     /gene="glnR"
                     /locus_tag="ACL8CL_001181"
     CDS             complement(2084..2440)
                     /gene="glnR"
                     /locus_tag="ACL8CL_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HGS1330433.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYENQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            complement(2517..3044)
                     /locus_tag="ACL8CL_001182"
     CDS             complement(2517..3044)
                     /locus_tag="ACL8CL_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HGS1330434.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            complement(3180..4376)
                     /gene="pgk"
                     /locus_tag="ACL8CL_001183"
     CDS             complement(3180..4376)
                     /gene="pgk"
                     /locus_tag="ACL8CL_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HGS1330435.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KYIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            complement(4466..9445)
                     /locus_tag="ACL8CL_001184"
     CDS             complement(4466..9445)
                     /locus_tag="ACL8CL_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HGS1330436.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAEEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYGYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTVQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTSDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKPRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGEWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQTPLATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDKGVEISGYDAQK
                     TGRQTLTLRYQGHEVNFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHETSTQDQVNDRLNKLTEAHKALNGQEKFTEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEGLTTAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELTFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNKVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVYENGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANEALIAGLASLGLASLALTLRRKREDKD"
     gene            9738..11369
                     /locus_tag="ACL8CL_001185"
     CDS             9738..11369
                     /locus_tag="ACL8CL_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HGS1330437.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            complement(11802..13409)
                     /locus_tag="ACL8CL_001186"
     CDS             complement(11802..13409)
                     /locus_tag="ACL8CL_001186"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HGS1330438.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGADNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDVEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(13799..14386)
                     /gene="rpoE"
                     /locus_tag="ACL8CL_001187"
     CDS             complement(13799..14386)
                     /gene="rpoE"
                     /locus_tag="ACL8CL_001187"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HGS1330439.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(14514..14963)
                     /locus_tag="ACL8CL_001188"
     CDS             complement(14514..14963)
                     /locus_tag="ACL8CL_001188"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1330440.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEAMY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNYASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(14968..15357)
                     /locus_tag="ACL8CL_001189"
     CDS             complement(14968..15357)
                     /locus_tag="ACL8CL_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HGS1330441.1"
                     /translation="MKVNIADLHPTQLYLSEKKLQDIQMLYQSAETIQVDPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(15385..16035)
                     /locus_tag="ACL8CL_001190"
     CDS             complement(15385..16035)
                     /locus_tag="ACL8CL_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGS1330442.1"
                     /translation="MKDKQTFLMKGSFALLLFVILGYMVKFYPEMLVNFDQSIQTAIR
                     GDLPDYLTILFRSLTRLIDIPVIITWVVITAFVFYRKRWKIESFFMLGNLALAGLLIV
                     TFKNIYQRPRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKTPVWRKIVQI
                     VLGLYLVSVLVSRVYLGVHYPSDVLASLCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(16025..16588)
                     /locus_tag="ACL8CL_001191"
     CDS             complement(16025..16588)
                     /locus_tag="ACL8CL_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1330443.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGPVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(16753..16987)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(17041..17544)
                     /locus_tag="ACL8CL_001192"
     CDS             complement(17041..17544)
                     /locus_tag="ACL8CL_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HGS1330444.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(17737..18567)
                     /locus_tag="ACL8CL_001193"
     CDS             complement(17737..18567)
                     /locus_tag="ACL8CL_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS1330445.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATIFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(18552..20234)
                     /locus_tag="ACL8CL_001194"
     CDS             complement(18552..20234)
                     /locus_tag="ACL8CL_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330446.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGIIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLISLLNQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHCPVDRIV
                     LINDGRILFNGSTDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLYIS
                     KGQVQLRTELVKETPELQSLFRLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKALSSFIQTEGRYLWEGQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEQEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQQGHTIVMITHDMQLMLDYSDRALVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(20336..20593)
                     /locus_tag="ACL8CL_001195"
     CDS             complement(20336..20593)
                     /locus_tag="ACL8CL_001195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330447.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     NHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(20600..21148)
                     /locus_tag="ACL8CL_001196"
     CDS             complement(20600..21148)
                     /locus_tag="ACL8CL_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HGS1330448.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(21163..22011)
                     /locus_tag="ACL8CL_001197"
     CDS             complement(21163..22011)
                     /locus_tag="ACL8CL_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HGS1330449.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     DQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            22199..23548
                     /gene="trkA"
                     /locus_tag="ACL8CL_001198"
     CDS             22199..23548
                     /gene="trkA"
                     /locus_tag="ACL8CL_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HGS1330450.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            23552..24991
                     /locus_tag="ACL8CL_001199"
     CDS             23552..24991
                     /locus_tag="ACL8CL_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HGS1330451.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(25116..25517)
                     /locus_tag="ACL8CL_001200"
                     /pseudo
     CDS             complement(25116..25517)
                     /locus_tag="ACL8CL_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359733.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS alpha-glucoside transporter subunit IIB"
     gene            26925..27209
                     /locus_tag="ACL8CL_001201"
     CDS             26925..27209
                     /locus_tag="ACL8CL_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330452.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            27202..27450
                     /locus_tag="ACL8CL_001202"
     CDS             27202..27450
                     /locus_tag="ACL8CL_001202"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330453.1"
                     /translation="MDKMKPVFQALNKELIQENLTLTIICVDGYVLEYHGLRATQDVD
                     AFYDQNQKINEIIARVGKQFNLNIHEELWLNNHVAKQI"
     gene            27546..27764
                     /locus_tag="ACL8CL_001203"
     CDS             27546..27764
                     /locus_tag="ACL8CL_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330454.1"
                     /translation="MMSIREQDLQDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
     gene            complement(28132..30456)
                     /gene="pflB"
                     /locus_tag="ACL8CL_001204"
     CDS             complement(28132..30456)
                     /gene="pflB"
                     /locus_tag="ACL8CL_001204"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HGS1330455.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
     gene            30805..31866
                     /gene="dinB"
                     /locus_tag="ACL8CL_001205"
     CDS             30805..31866
                     /gene="dinB"
                     /locus_tag="ACL8CL_001205"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HGS1330456.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAENFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIRKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITLTGF"
     gene            complement(32013..32858)
                     /locus_tag="ACL8CL_001206"
     CDS             complement(32013..32858)
                     /locus_tag="ACL8CL_001206"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HGS1330457.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKARAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
BASE COUNT         9767 a         7093 c         6110 g        10020 t
ORIGIN      
        1 tatgtgcttc aagtatataa aaaggcgtaa aaatacgcct atagataatg gggttgaaat
       61 aggtttattg ttgatgagat tgtagataat tcaatttttt acttccaatc gaatattcaa
      121 aacctccacc ttttctgcct gtaattgttc atcataaaat tcaatatctt caggattttc
      181 cccttgacaa cctcggcaga aatattcttc cgctcgatca ggattcaaaa atcgacaagc
      241 acaaacaaaa cagtcgccat catcatttat tgagataata tagtagattg aaataagatg
      301 taaacaaatc gattaggaaa gttaaattaa tttctagaaa tttttagcag atgtagtgta
      361 ctattctagt ctcaatttac tatggcttca aatatatctt tcgaaaaaat atttacagat
      421 gtgtaatttt gaagcttgca aaagttagta aacttgtaga tttcgatttg aagtaacttg
      481 ttttcttgcc cgatattgtt tttgaaattg aatttttcca tagtgactcc ttgattttct
      541 tctacacgtc tgatgataaa tctaattcgc aaaagagtca agaggatttt tcgaaaaata
      601 aatagcgacc gaaatcgcta ttttaagggt tataggtatt tgatggctta gactgctgtg
      661 tgactgttta cccacaggca atctttcttc tatattagta ttagtaaagg tctaaataat
      721 tatcaatttc ccattgtgaa acgaaggttg cataacttgc ccattcgatt cgtttggctt
      781 caaggaagct agtgtagatg tgatctccga gagcagcttt aaccacttca tcttctgtca
      841 aagctttcaa agcgttgtga agagttgatg gaaggtctgt aataccagct tccttgcgct
      901 cttctgctgt catgatgtag atattttctt cgataggagc tggtgcttcg attttatttt
      961 caataccata caaaccaact tccaaaagaa cagccatagc aatgtaaggg ttcgccattg
     1021 gatccactga acgcaactca agacgagttc ccataccacg tgaagcaggt acgcgcacaa
     1081 gtggcgaacg gttacgacca gcccaagcaa tgtaaacagg cgcttcataa cctggaacca
     1141 aacgtttgta tgagttaact gttgggttca tgatgacagt atagttgtaa gcatgcttga
     1201 tcaaaccgcc taggaaatgg taagctgttt ctgacaactg cattcctttt ggatcatttg
     1261 gatcaaagaa ggcgttattt ccttctgcat caaacaagga catattacag tgcatacctg
     1321 agccagcaat accaaatttt ggcttcgcca taaatgttgc gtaaagtccg tgtttgcgag
     1381 caatggtttt aacaacaagt ttaaagattt gaatcttatc acaagcacgg agaacttcat
     1441 cgtacttaaa gtcaatctca tgctgtccaa ccgcaacctc gtggtgactc gcttctactt
     1501 caaatcccat tttggtcaag acattcacaa tctcacgacg tgtgttgtcc gcaaggtcag
     1561 taggtgccaa atcaaagtag ccacccttgt cattcacttc aagtgttggg tccccatttt
     1621 catccaactt aaataggaag aattctggct ctggaccaag gttgaaggat ttgaatccaa
     1681 cttcttccat gtgacgaaga gctcgtttca aattaccacg agggtcaccc gcaaatggtt
     1741 caccttctgt tgtataaaca tcacagatca gacctgcaac acttccattt tcatctcccc
     1801 aagggaagac tgtccatgta tccaagtccg ggtacaagta catatccgac tcattgatac
     1861 gtacaaaacc ttcaatagaa gatccatcaa acataacctt gttcgacaag accttatcta
     1921 actgttcatc tgtagcagga atttcgacgt ttttcatggt tcccaaaata tctgagaaca
     1981 taagacgaat aaaggtaaca tttttttcct tgacttcacg acgaatatct gcagctgtga
     2041 ttggcataag ttttctcctt aatctatgac tacttgcggt tgcctaaccg cgaccaaaag
     2101 gtgactgtac tgaagcaaaa cgcccctgtt ggaggagttc attgtgaagt gcacgacgta
     2161 cttcagtctg actaaccgct ttcttggatt tcgcttcacg ttcagcatat tttttcttaa
     2221 tggcagcgat attataacct tcagagatat aatctttgat ttcaagcaga cgatccatgt
     2281 cattcaagga atacatgcga cgatttcctt cgtttcgatc gggcttgatc aactcttgat
     2341 tttcataata acgaatctga cgcgccgata gatcggtcaa cttcataaca ctgccgatag
     2401 gaaaaacagc catatttcgg cgaaattctt tttccttcat ttacaatttc cttctttctg
     2461 tctattatag tctaaaaaaa gacaaacgtc aattaataat gttataaaat gtaacattat
     2521 ttttcttttt tctctaaaaa gagacgaata cgatcaatat cgtaatttac gataattgcg
     2581 acaaaaactc ccataaatgt ttctaataca cgcacaaaca cgtacaaaat tgtctcacca
     2641 cttggaattg atagggtaat aattaacata gctgctacac caccaataac ccctgctttg
     2701 ttattcatgg ctacatttgt cataatggtt aacatggtgc agattggaac aactaccaag
     2761 gtcacccaaa aggcttcgtg gaaaaaggta tttaataaga agaagaccaa ggcatagagt
     2821 ccaccgatac tatttcctag aatacgcgaa gtcccaaaat gaacactctc atcaaaactc
     2881 tccctcaggc taaaaacggc tgtcaaagca ccaatttgaa gacctttcca gccaaaaaag
     2941 ccaaaaatca agagaactag aaaaacagca atacctgttt taaaggttcg cataccaagt
     3001 ttgaactggg atttatcgaa tttatatttt ttaaaataac tcataatctc aactttctat
     3061 ttccatttta tcataaatcg gtgattttta tgagtaatag ttgagaggaa gcgtttttat
     3121 tttaagcaaa agaaaagagg aactttcatc cctctcttct ttgatttatt tataaaatct
     3181 tatttttctg tcaaggctgc aagtcctgga agaaccttac cttcaagaag ttccattgat
     3241 gctccaccac ccgtactaat ccatgagaac ttgtctgcac ggccaaggtt aatcgctgcg
     3301 gcagctgagt caccaccacc gatgattgat ttaactcctg gttgtttcac gatagcgtcc
     3361 atcacaccga ttgtaccagc ttggaaatct gggttttcaa atacacccat aggtccgttc
     3421 catacaactg ttttggcacc agtcaaagct tcgtcaaatt tggcgataga ttttggaccg
     3481 atgtcaagac caaggaagcc ttcagaaact gcttcacctt cagtgtcacg cacttcagtg
     3541 taaccagcaa atgcgttagc ttcttttgag tcaactggca agatcaattt accatttgct
     3601 ttttcaagaa gagctttcgc aacatccaat ttgtcttctt ctacaagtga gttaccgatt
     3661 tcgatacctt gtgctttgta gaatgtgtaa gtcatcccac caccgataag gactttatca
     3721 gctttttcaa gcaagttttc gataacaccg atcttgtctg aaacttttga accaccaagg
     3781 atagccacga atggacgttc tggagtttca actgcttctt ggatgtaggc aatttcgttt
     3841 tcaagaagga aaccagcaac tgctttttca acgtttgctg agataccaac gttagatgcg
     3901 tgtgcacggt gagctgtacc gaatgcatcg tttacgaaga taccatctcc aagtgatgcc
     3961 cagtatttac caagttcagg atcgttttta gattctttct tgccgtcaac atcttcgtaa
     4021 cgagtgtttt caaccaagag aacttgtcca tcttcaagag cgttgattgc cgcttccaat
     4081 tcagcaccac gagtgacacc tgggaaaaca acatcttgac caagttttgc tgccaagtca
     4141 gctgctacag gagcaagtga tttaccagct ttatcagctt cttctttcac acgtccaagg
     4201 tgagagaaaa gaattgcacg tccaccttgt tcgatgatgt acttaatagt tggaagagct
     4261 gctgtgatac ggttatcgtt agtgattacg ccatctttca atggtacgtt gaagtcaaca
     4321 cgaacgagga cttttttacc tttcaagtca acgtctttaa cagtaagttt tgccatgtta
     4381 caaaaactcc tttatttatt ttattagaaa ctattatatc acactttgga aatataagga
     4441 aagatttcac aagatttttc gatatttaat ctttatcttc tcttttccgt ctcaaggtca
     4501 aggctaaact agcaagacca aggctggcta agcctgctat tagggcttca ttagcatcag
     4561 ctgttcctgt atttggcaat tgtttgcttg cttcttctga tttaactgct gtattttgag
     4621 ctggtttttc ctgtgtagct actactgctg gtactgtttt tacttttgtt ccaatttcaa
     4681 caatttctgg aatcgcttct tggatgactt ctgttgaacg aagacgacgc tgaccgtttt
     4741 cataaacttc aaagacatgt cttaacagtc catctcgtcc ctgtatgatg actcgttgtt
     4801 ccccaactag catctcagca ttttcatgct cttgacgatg aaaggcaacc ttttcctctt
     4861 gaacttccaa tttaggagcc ttttgttgat cagaagccgg ttgataagta ggttttggaa
     4921 ggactttgtt ttgtggtgct ggtttagcag gttgtggttt ttcagcagat tcataaacaa
     4981 aggcataatg agtaaagtga ggtgctgtaa agataacatg actatccgtt tgttcaaaag
     5041 tcaattctac tgcctctttg ccttcaggta agaaaaatac tttcttaact ttcttatctt
     5101 tttcaattgg gattttcaca atagaagcat aagagagatc aacatcttga cctttttcat
     5161 ccaaaccttc tatttcaaag acatgagcat cttctccagc aaagtatttc ttctcttcag
     5221 cacttgcttg aacacgctct actttcaaac ccttgatgac agtcttctct ttatttgaga
     5281 agtgtacttc aacacctgtt ttactatcag aaaagactgc tggcttgtct tctttcaata
     5341 aagcaaccag atcttttaga ctctgtttcg ctgttgtaag cccttctgga ctcaaatcta
     5401 ctttttcaac caaggccttg tttttctcaa gaagcggagc tagagcagaa cctgaaggat
     5461 ggtttggttt ggcagccaag agttcttgaa cctcacctgt taaacgatca agctctgtct
     5521 tttcttccgt aaatttctct tgaccattca gagctttatg agcttctgtc aatttattaa
     5581 gtcggtcatt gacttgatct tgggtgctag tttcatggtc tttcaacact tgttcggccg
     5641 cttcaattgc tttcaagaaa gcttctttta cttctggtga agcaaagtta tagaccacct
     5701 tgttcttagc agcttcaact tctgctaatt tctgtttgag gtactcatcg ttcaatgctg
     5761 cttttggaga taccaaaaca tcaaagttga cttcatgacc ttggtaacga agcgtcaagg
     5821 tttgacgacc agtcttttga gcatcataac cagaaatttc aactccctta tcagtaaagg
     5881 aatgttcttc catggtgtca ttgctataag ccactgcaaa gcgtccttca gacaagtcta
     5941 agctatcacc aactaggtaa tcttttttcg gttcctgact tacttcaatt cccaaaatag
     6001 ttttcggact tgcttcgtct tggccagtga cagtcactga caaattagca tttaccggtt
     6061 gtcccaaata ttggagagta agattctgtt ctcccttatg atgcgtatca aaacctgata
     6121 ctgatacacc tgcgtgagtt aggcgaatga gttcgtcctc agttcctcct tcatactgaa
     6181 ctcgaagaac accacctcta aggtccaaat cctccccttc ggcgtagctt gttttcttag
     6241 gtcctgtttc taggctgact gacttgattc ttctgtcatc ttttggaacg gaaactgcta
     6301 ggacattact aatttccttg cctggcaact gggtacgata ataaagaaga ccagattgat
     6361 ttgtcaaatc caatggtgca cttgctaggt ctcctccaac ttcgctcttc aaggttgcga
     6421 gtggagtttg agaatttgga ttatcataaa cggtaatagt tgctccagcc ggtacatctg
     6481 caaagccaac ttgtaccttg ttattgccta gagaacgggc tgcagccttg gccatcggaa
     6541 tattgacact ctcagtatca agcttttcat acattttcca gttatagata cgaatagcct
     6601 tccatggagt tccattgtca gaagtgacaa cattcaagcg ccagtcttga gcagtgattg
     6661 gtttatcaag agtgatatct gtcacgtgtg ctttgttgcc acggacttcc ttggctagct
     6721 tccactcacc atctgcatct ttataataaa ggtcaaagtc tttggtgttc atcaagccat
     6781 cgttaacaga ctcaccacca gcccctgcat gatccatgac ccatctaaca acggtacgtg
     6841 gcttggtcaa acgaatatcc acactaccac tcaactgagc tgaagaccat ttatctgaca
     6901 agctagtaat ggtaccgttc aacatacctt caataccttc tccaccttca gtcttaggga
     6961 aagtactatc aataactgtt gcacctggaa cgatattttc agctagtggt tttggtaggc
     7021 tggtatcttt tacagtcata ccccaatcaa aggttgtggt tgcagcttct gaacgaactc
     7081 catttttacc gactgctaca accttcagtt cttgagttgt accctgagca cttgctgagc
     7141 ggctaacttt tggtagataa atagttgtag aagatgagcc agttagtaat ttccagctgt
     7201 ctccatcttt ttcataaact tcatagaaat ctgcatcctt gttgccttta aattgcacaa
     7261 ctgcttccgc ttcttgggca tttttaagag attgtttcac tacagaaaag cttgtcggcg
     7321 attgtggctc ttggtgattg tccgagatag ttaattgtcc gaggttgaac tgataatctt
     7381 ttacagcacc ttcatgctcg aaaaatagtt tgactgcata gatggtttta cccgctagtg
     7441 agctgagatc aaattcttca tttgtccagt tgtcagaaag ggttagctct ttccatgcat
     7501 ctgcatcatc gaatttgtag tctgaagttg tagagaatgc catataaact ttagaacctt
     7561 ttcctccctt gtgggcaaca cgaagtttgg tcttctcagt tacttctaac ttagtagaat
     7621 aaagtctcac atcctgaact gtcttaccgg ctacatcacc agagaattta agggaatttc
     7681 cgccattata ggcatctgta aaatcatatt ctgcacgaag tttttcccct gttgaagtct
     7741 gccaccagcg ccatgttgga agaacacctg atactgaacg ataattccac tcagaatcct
     7801 tagaaacctt accatctacg aaccattttt taccatgacc tgtattaaaa gaagtagtaa
     7861 aagtattacc taccgctggc gtacggtccg caactaggtt agcaatacca taccaatctt
     7921 tgtcacctgg tttttggcca gtggggtctc cttgataacc tgtaaagaag atatcttcat
     7981 ttttatgata atcttcacca gtttttccta aacttgtaat ggtatctggg gcaaataaac
     8041 caagagaaag gcgcaatttc ccattttcgt ctaaaatgtc attccactta acctttgtct
     8101 tgtaggaacc accctgttgc aattccaaac ctgcaaatac atcataagga ttacgaccaa
     8161 tccagttggc agttgcaata gtgtaatcat ttttagcctt atcccagtta aagttagcaa
     8221 agaagttatc tgccggaacc ttatctcctt ctggttgcat gaattggtag ttgtattctc
     8281 ccaaaccatc ttgatggtaa cgtccatagt tataggtcat ggcatcatac caagaatact
     8341 tgattggatg gtttacctta gcagcatatt ccttgctata gagcataaac tggcgcatct
     8401 tttctccaag aggtttaacc aaatctccag ttgtttcttg gttgatgaaa tagccatcat
     8461 agccataata ctttgccatg tctaccaatt tacgagcaat tgggaagcta ccatctgcgt
     8521 cttgcttcaa agcttcagca aatctttctt gatctgcaat actattagac cagttgaaga
     8581 agagtgtacc gtatacagga accccgttac ggtgacctgc atcaataacg tcaggagttg
     8641 gtacgagacc ttcccagaag accattgaat ctagatattg ccaatagtca aaagcatagg
     8701 ccttgaactc ttctccacca acagaagcat ggtcttttgc tttagaatta gtgtttgata
     8761 aggcttgaac ttttgcttcc ttgctagctt tttcattgac taaatgacct gtccgacgtg
     8821 aagcgaggac aacagatccg cggttaatag catcatcttc acgagcacct ggttcccatt
     8881 ttaacaattc ttcccaagaa ttaaatttga tttcctttgg ttttaatgtt ttttctgtat
     8941 ttttagggac atctggctgg acttcttttt cagtcttatt agcttcttct gctggtttag
     9001 actcatcccc ttggttagtt gcttcgggct ttgcttctgt ttccttgtca gacttaggtt
     9061 ttgcttctgc agatggtgtt tctgcaacca cctcacttgc tttttcctct ggtttagctg
     9121 cgctagctgc ttctgcagtt tcagtagttg cagcttcatt gcttgctggc gaagcagttt
     9181 cagatgctat cgctactcct tctgcctttt ctagcttgtt ttctctagca tgttcccctt
     9241 cattctgctt atcagcttca ttggtcgaat gctcactctc tcctgaaaca agctctgtat
     9301 tagctccgct attttcagga actgcagttt cttcagccaa ggctggacca gcaaataaaa
     9361 cagcaccaat catcagcgag caggctccta ctgataactt acgaatactg taacgacaac
     9421 gtctttcaaa aaatggattc ttcattttct cctcctaaaa gattgatttt gttgttttca
     9481 agaaagcgct ttgtattctg tggtttttta tcaaactcta gaaccatgat aatcacattt
     9541 ttatcctaaa acatttttag acaaaaaaca atagctgttt tcacaaatct aaaataaggt
     9601 taaaaactct taaaaataat acacttatca attataattt tcttttacaa acttgacatt
     9661 aacacttgtt agatttactt ttatccttta aaatagaata ggagatattc cgcgattatt
     9721 tttcggagga aaaagaaatg tccattaatt ggcaggaaat tttatttcac tttttaggtg
     9781 gcctggggct attcttatat agtatcaaga ccatgggaga cggtttgcaa caagctgctg
     9841 gagatcgcct tcgtttttac attgacaaat atactagtaa tcctttcttt ggagttctgg
     9901 ttggtattgg gatgactgct ctaattcagt ctagttctgg tgtaacagtt attacagtcg
     9961 gcctggtcag tgccggtctc ttaaccttac gtcaggctat cgggattgtc atgggtgcta
    10021 atattgggac aactgtcaca tcctttctca tcggttttaa attaggtaac tatgccctac
    10081 ctatgctctt tatcggtgcc gtctgtctct tttttacgaa aaatcggaca gtcaataata
    10141 tcggacgcat cctctttggt gtcggtggta tcttttttgc cctcaatctc atgagcggcg
    10201 caatggctcc actcaaggat ttacaggtct ttaaggacta tatgattgag ctaagtaaga
    10261 atcctgtttt gggtgtcttt gtcggtactg gcttgacctt gctaattcaa gcttcttcgg
    10321 ctaccattgg gattttacaa aacctctacg ccggcaatct aattgaccta cagggagctt
    10381 tgccagttct atttggtgac aatatcggga caaccattac agccatcatt gcctctttag
    10441 gggctaatat tgcagctaaa cgggtagcag gagctcatgt tgccttcaac gttatcggaa
    10501 cagtcgtctg cgttattttt ctagttcctt ttactgtcct gattcattgg tttgaagcta
    10561 cgctaaatct agcaccggaa atgaccatcg cctttgctca cggaaccttt aatattacca
    10621 acaccattgt ccaatttcca tttatcggag ctctggctta ctttgtaacc aagattattc
    10681 ctggagagga cgaggttgtc aaatacgaac ccttatatct tgatgaacat ttcatcaaac
    10741 aggccccatc tatcgctcta ggaaatgcta agaaagagct cttgcactta ggaaactacg
    10801 ctgctaaagc ctttgacctt tcctataagt acatcattga cttggatgaa aaagttgctg
    10861 aaaaagggca taaaaccgaa gaagcaatta acaccatcga tgagcaatta acacgttatc
    10921 tcattgccct ttcaagcgaa gctctcagcc aaaaagaaag tgaagtgctt accaatatcc
    10981 ttgattcctc ccgtgatttg gaacggattg gagaccacac ggaggctcta ctcaatctga
    11041 ctgactatct tcaacggaaa aatgttgaat tttctgatgc cgccttgaaa gaattagagg
    11101 aagtttaccg ccaaactagt gactttatca aagatgctct ggatagtgtg gaaaacaatg
    11161 atattgaaaa agcacgcagt cttgtagaac gtcatgaagc aatcaataag atagaacgtg
    11221 ttctcagaaa aacccacatc aaacgtctca acaaaggcga atgttcaaca caagctgggg
    11281 tcaactttat cgacatcatc tcacactaca ctcgtgtatc agaccacgct atgaaccttg
    11341 ctgaaaaggt ttttgcagaa caaatctaag aaccaagaag ctatccatca taattggatg
    11401 gctttttact ttttcctaag caagactagg ctgaatgaaa ctgaaagagt attctgtaga
    11461 tatatagtat attgaaacta gaatagtaca cctctatttc taaaacattc ttagaaatcg
    11521 atttgactgt cctgatagag atgttcacat cttatttcaa ttcactatac aatccagttc
    11581 ctaaaacttt gctagaaatt gatttggctt tccgaattaa tttgttcaaa ttttatttca
    11641 tttcaatata ctgataaatc cattcactag cacagggcaa gagggagtcg ctatttattt
    11701 caacggtagc aggtacatac agaggactcc tatctcagat atactgcaat atcatcgtat
    11761 gcaaaaaacc tctgagattt ttctcaaagg ttctgatttt gctaattgct gttctcaacc
    11821 gctgcagtaa caaaggcagt gtagagttct tctggtcggt ttggacggct tgacagttca
    11881 gggtgatact gacaagctac aaagaattta ttttcaggaa tttccacgat ttctaccaaa
    11941 cgattgtctg gagaaactcc tgaaaagaca aaacctgctg cctcaaactg ctcacggaag
    12001 gcattattaa actcataacg gtgacggtga cggcgttgca ccacttcttg attgtgataa
    12061 gcagcagcag ccttagagcc acgtttcaac ttagacggat aaagtcccaa acgaagggtt
    12121 ccacccatat cctcaatatc aatctgatca cgcatgatat caatgatagg gtattttgtt
    12181 tctggtgcaa gctctgcaga attggcacct tcaagaccta aaacgtgacg agcaaactcg
    12241 atacatgtca actgcattcc caagcagact cccaacattg gaacatcatt ttcacgcgca
    12301 tagcggatgg cttggatttt cccttctgta ccacgttgac caaaaccacc tggtacgatg
    12361 atcccgtccg catcagacaa gagttctgct acattctctg ctgtcacatc attggcattg
    12421 acccaattga ttttaacttc tacatcattg acatagccag agtgtttcaa ggcttcgacc
    12481 actgagatat aggcatcttg caactccaca tacttaccaa caagggaaat cttaacttgt
    12541 ttcttgaggt tcatgacctt gtccaccatg gctgaccatt ctgtcatatc cgctgctggt
    12601 gcgtctaatt tcaaatgatc acaaacaatt tggtccatcc cttgtgcctg caagttcagt
    12661 ggaatttggt aaaggtgttc aacatccaac gattcgataa cggcttctgg tgccacatca
    12721 cagaactggg ccagtttatt tttaattcct tgaccagctg gctcttctgt acgaataacc
    12781 aacatatttg gttggattcc caagccacgc aattctttga cagagtgttg ggttggtttg
    12841 gttttcattt caccagcagc cttgaggtaa ggaagcaagg ttgtatggat atacatgaca
    12901 ttatccgcac ccacatctgc cttcatctga cgaagagcct ctaggaatgg caaggactcg
    12961 atatctccta ctgttccacc aacctctgtg ataatgacat cagagtcggt cgttagagcg
    13021 gcacgcttga ttttttcttt caaagcatct gtgatatgag gaatgacttg aacagttgcc
    13081 ccaaggtatt ctccacggcg ttctttacga agaacttcac tgtaaatttt cccagttgtc
    13141 acgttggaat atttgttgag attgatatcg atgaaacgtt catagtgacc caagtccaaa
    13201 tctgtctcag ctccgtcatc tgtcacaaaa acttccccgt gctggtaagg actcatggtt
    13261 cccggatcaa tattgatata agggtcaaac ttttgaatgg ttactttgag accacgattt
    13321 ttcaagagac ggcctagact cgctgccaca atccctttcc caatggacga taccacacca
    13381 ccagttacaa aaatatattt cgtagacata gattcctctt tctaaaatgc tcaaggtctt
    13441 gttaactacg aggggatata gaaaacaaga ccctactaat aagaataatc tgggacgact
    13501 gcaccagatt cacaactaat actcttcgaa aatctcttca aaccgcgtca acgtcgcctt
    13561 gccgtagata tgtgttcctg actttgtcag tcttatctac aacctcaaaa cagtgttttg
    13621 agcagcctac ggctagtttc ctagcttgct ctttgatttt cattgagtat aaaatacaag
    13681 aagataactt cttgaaaaaa caaaaatagc tccctaagaa ctagggagcc ccgacctcta
    13741 aaagaggtgc ccaaacaata tgatacctaa aaataggata attgtcaata gctaactctt
    13801 attcctcgct ggtttcagcg tcgtcatctg aaaactcgtc gtcgtcttcg ttgagatcca
    13861 catcttctcc caagtcatct ggagcgattt cgttgatttc tgcatcataa gcttccactt
    13921 catttttttc atcatctgga ttttcatcat cgtatgaaag agctggatct gcttcgtatg
    13981 catcttcgtc ttctggatca tctgcattgt agtcaatggc atctgaatca ccatccataa
    14041 aggcattgac acgttttttc ttagcttttg gtgctacttc atcgtcgtca ttttcttcaa
    14101 gagcgatgat ttcttcgtcg atttcgtcca caccatacca tgaacgaaga ccccatttgt
    14161 tgtccccaag tgagatgaag ctaccgtcaa agttcaactc tgtgtagaac aaaggcaaag
    14221 cttcgcggat atcgctgttt gatgttccaa ggtagttttg aatttcgttt acaagatcgc
    14281 taaaatgcat ctcatgatcg cgaccacgaa gttccaagat agcacgcgct acctcaatca
    14341 tagatagttc acttttttct tgcccagcaa atacttctaa ttccaaagcg tttctcctca
    14401 tttatactac tatcgccaga gcgaacagac tctgacctca ttttatcatt tactctttat
    14461 tttacgataa ttttgcggaa tagtcaaagg ttaaggggga gaaagtggca ggattagact
    14521 aattccaata taaaactcct tcctttttct gttgctccat tttccacaaa tccaagcgac
    14581 ttgaaacacc tcctagaagc ataattgtag gtgtagattt ccttgactct caattctttc
    14641 catcctttta ctcgagccaa ttcaatcaaa gcacttagaa tcttttttcc aagtcctcga
    14701 tgttggtaag cggaattccc aatcacaatg gggagattat cctgagatag tgtaatatcc
    14761 ccaattggaa accattctcc cttctccttg acttcaatcc aaaaaagctc accatgccga
    14821 tccaaatagg aatacatggc ttccaaggtc gcttgactgt aaggaagctt caccccatct
    14881 acgaggtaaa ccaagttcac atcctgatac caagcaaaag cttcctcaca atgattgacc
    14941 ttataataag gaacaaaaca caatgcacta ccttttcaac aaaagagttg ctgcttgatt
    15001 aaaaccatca caccagttat accattttgc ttcataccca tcttgagcta ggatacgatc
    15061 ttctaaatca aaaacagagt aaatctttct ttcctcgcaa gcttgcgcat agagatgata
    15121 tagttcatca ccaccatctc tatcccactc agcagaaatc gtatcccgac ctgccaataa
    15181 agcctgataa gccctgtgat gcccatctgt aatcagcaaa caatctccaa aggcaagaat
    15241 actgattgga tcgacttgga ttgtttctgc cgactggtaa agcatctgaa tatcttgcaa
    15301 cttcttttct gataaatata gttgagtcgg atgaagatct gctatattga ctttcatttc
    15361 tttctcctca agggaattcg atactcactt ctgtttgcct ttaaatcgcc attggaagcg
    15421 gagcttgtca tagaagggaa actcgataaa caggactccc aagcccacac agagactggc
    15481 aaggacgtct gatgggtaat gaactcccag atagactctt gataccagca cactgactag
    15541 gtagaggcca aggacgattt gtacgatttt tctccagact ggagttttaa ttcgttgact
    15601 gagaatgaca atcagagtac ctaccatcaa agttacagcc agagaatgcc cacttgggaa
    15661 ggaaaatccc ttctcttcca ccagatgtaa gatagctggt cgtgggcgct ggtagatatt
    15721 tttaaaggtc acgattaaaa gacctgccaa agccagattt cccagcatga agaaactttc
    15781 tatcttccat cgcttacgat aaaagacaaa agctgtaatg acaacccaag tgataatcac
    15841 tgggatatca atcagacgtg tgagggatcg aaaaagaata gtcaaataat ctggtaagtc
    15901 tcctcgaatg gcagtctgaa tcgattggtc aaaattgacc aacatttcag ggtaaaattt
    15961 gaccatatag ccaagaataa cgaaaagtaa aagggcaaaa ctgcccttca ttaaaaatgt
    16021 ttgtttatct ttcatagtgt tttaaggttg gtttcaaaag gacgtacaac aaccagaatg
    16081 aaacggaaaa gattacaccc tcaatcaagt taaaaggtaa taccatggtc attaggtagt
    16141 tggaaagtcc caaaattttt ccaatatcaa agttagcaaa cttagcgtac aaaggaacag
    16201 cataaacata gttgagaacc aacatggcca aggttaaacc aacagttcca gctagagagc
    16261 ctagtaggaa acgaagggtt gtccgttcct ttttccaaat caaagcaaat acgatgacaa
    16321 aaactcccaa agctacgata ttcatcggca aaccaatgta agtattcact ccttggctgt
    16381 taagaagcaa tttcaagagt gagcgaagca agagcactcc tagagcagca ggcaaatcca
    16441 tgaccaccgg acccacaagg actggcaaga tactaaattc gatcttgagg aaagatgccg
    16501 ctggtaaaag cggaaagtca aagtacatca gcacaaatga gatggctgat aaaattgcaa
    16561 tggtcgaaag tcgacgtgtg tttgtcataa caggttcctc caattttcta taaaatcaga
    16621 agaagttgga aaggattcct ctatctattc tcacttttta tatcccaaaa gttccctctt
    16681 actctattaa agaaaaacaa agcaagtggt tacaatccgg ctataaatct atcaaaacag
    16741 acaaagctat tctttcgtct tctcccatcc agactatact gtcggttgtg gaatctcacc
    16801 acatcagctt gcgctcgcgg acttctttaa agagaaggaa atagagattt ttcttttaga
    16861 ctaggcgaca agctgtaggc ataacttctg gttaggcaaa gcttgtcaac aaagtataaa
    16921 aggaaaagat catttcctag ttaaagtcac cgccggtcgg gaatcacacc ctgccctgaa
    16981 gactctttta tcataacaaa aaacgcttgc aagcgcaagc attttgttca ttttcatttt
    17041 ttatttcaat ttatccactt cataggtatg gacgagctca agacctgcaa agttctgctg
    17101 gcgaagagcc tcatagacaa tcatgcagac ggtattagac acattgagac tgcggacatg
    17161 ttcatcattc atgggaatac ggagagcctt ctcaggatgt tctcgcataa aatcttcagg
    17221 taagcccttg tcttcacgtc caaagagaaa ataatggtcc tcgtcagtcg ataaatccac
    17281 ctcagaatag actttttccg cgaatttcga aatcagatag agttttccct tcatctgaga
    17341 tatgaaatct tccaaactct cgtaaaaata aatctctagc ttatcccagt aatccaatcc
    17401 agcccgcttc atcttgcggt catcaatagg aaagcccata ggcttgatga tgtggagggg
    17461 agaattggtc gcagcgcaag tacgcgcgat attgcccgta ttttgtggaa tttgaggttc
    17521 aaataataca atgtgatttg tcatgacttg cttcctttca ccattgcaaa aaaatagcca
    17581 cactgcccgg agtcaagctc agcaaacagc gtggttaagg catcgttaac ttacctcaca
    17641 acaggtttga agtaaatcag cgaaactact ttcttagtat aacactttca gaatcattgt
    17701 caatagaaat gacttgattt tttcaatttt ttcaagctat ttccaagggt tgtaaaatcg
    17761 tccctgattc tgcaagataa gtagtaaact aactactaaa aacaaggttg ccaagagcaa
    17821 ggtaatatag tctccttttt tcaaggcctg ataactatac catgtgcgtt ttttctcttt
    17881 cccaaagcgg cgaagctcca tggcagtcgc aatggtatca atgcgttcta gcgagctaaa
    17941 aatcaagggc gtaataatga gcagattgcc tttgattcgt tgcataagag aagctttctt
    18001 ggataattcc atcccacgcg cctcctgaga catcttgata gtaaagaatt cttcctgcaa
    18061 atctggaata tagcgcaagg tcaggctgac agaataagca atcttatagg gcacaccaat
    18121 ttgatttaaa ctggaagcaa actgactagg atgggttgtc atcaaaaaga taatagccag
    18181 aggaatggtg caaagatact taatggccaa atttagcaga taaaagagct cctggctggt
    18241 tagagtgtag acaccgattc cctgccaaat cacacttctc tctccataaa gtccaacccc
    18301 atactcggga gaaaagagat agaccatcaa aacgtttaaa acggcaaata tcgtcgcaaa
    18361 aacggctaca aaggaaacat ctttaaagcg aatttctgat aaatagagga gaaagactga
    18421 aaagatggca atcagcaaga gcattctggt atcatagcta atcatggccg ccaatgatac
    18481 cagaatgaaa aagagaagtt tcccagctcc tgacaagcga tgaatcacag tatctctatg
    18541 ctggtaaccg attaatttag cttgcatccc tctctccttt ctttgtaaaa tgccgttaaa
    18601 tccagtggat ccacatctag tttcttagcc aagttaaaga tggaggtttc ttttagattg
    18661 gcttttacta acagctcagg atcgctcaac agactggctg gaacagtatc ggcaatcaat
    18721 tctccatcca ccatgacaag ggctcgatct gaataatcca gcatcaattg catatcatgg
    18781 gtaatcatga caatggtatg cccttgttga tgcagttctt cgagaaattc cataatctca
    18841 gtatagttct tctggtcttg acccgcagtc ggttcatcta ggaggataat ttcagcgcct
    18901 aagaccaaaa ttgaggcaat agtcacacgt tttttctgac caaatgacag ggcagaaatg
    18961 ggccaattac ggaattcata gagaccacag attttcaagg tttcatagac tctcgtttca
    19021 atttcctgct cgtccacacc tcgcaaacgg agtcccagag ccacctcatc aaaaatcata
    19081 ttggttgaaa tcatttgatt gggattttgc agcacatagc ctactcgttc cgcccgctct
    19141 gcaacagaat ctccttttat atcctgtcct tcccaaagat agcggccttc agtctggata
    19201 aagctactca aggccttggc tagagttgac ttccctgctc cattttttcc gacaatggca
    19261 atcttttcac cctttttaat atctaaatga agagatttta aaatcggtct atcatcataa
    19321 gaaaacgaca cgtcctctag tctaaagagt gactgcaatt ctggggtttc ttttaccagt
    19381 tccgtccgca actgaacctg accttttgag atatacaagt tatccagatc tgctaattgt
    19441 tcttccttga ctaaatccac acccaattga cggagagtgg tgagataaag gggttcgcga
    19501 attccatttt gggtcaacaa atcagtcgcc agtaactggt cagtgctccc attaaaaagg
    19561 atacgaccat cgtttatcaa gacaatccga tccacagggc aatgcagaac atcctccaaa
    19621 cggtgctcga taataagggt cgtcgtcccc tcctccttat gaatctggtc aatcaattcg
    19681 ataatatcct gacctgactt gggatcaaga ttggcgagtg gctcatcaaa caagagaatc
    19741 ggactctcat caatcaagac accagccaga ctgactcgct gcttttgccc accagacaaa
    19801 tcctgaggac gctgattcag taaagaaata aggtccagct tttcagccca tttataaaca
    19861 cgacctttca tctcatctag ggctgtcaca tcattttcca gagcaaacgc caaatcttct
    19921 gccacagaca agccaataaa ctgcccatct gtatcctgca aaactgtgct aaccagatga
    19981 gacttatcat agatgctcat atcaaaggct acttgaccct ttatcaaaaa ttctccatat
    20041 gtctgaccct tgtaaatatt gggaataatc ccattcaaac actgacccaa ggtagattta
    20101 cctgacccag atggtccaac aattaagact ttctctccct tgtaaatggt caagtctatc
    20161 ccttgcaagg tcggttcttg ttgtgtttca taccggaaag agaaatcctt ccactcaatt
    20221 atagcttctt tcatcttact ctcttcattc gcttcttaga cttctatttt atcataaatc
    20281 aagcccttct tgcagtctct cctcttaaaa tcttagcgcc aaaaagattc ctatcctagc
    20341 ttacttgcct aactaatcta taaacatcga aaaagactag ttgcccagcc ttccccatca
    20401 ttttatactc ttcgaaaatc tcttcaaacc acgtcagctt cgccttgccg taggtatggt
    20461 tactgacttc gtcagtttca tctacaacct caaaaccatg ttttgagctg acttcgtcag
    20521 ttctatccac aatctcaaaa cactgttttg agcaacctgc ggctaacttc ctagtttgct
    20581 ctttgatttt cattgagtat tagtcctttt tcaaacttcc tgcacgagtt tgagttcctg
    20641 cataggcaag taagagaaga gttcctgcaa tagctacaga tacaccattg gcaattcccg
    20701 caacaatccc ttgtgcaaat actttttctg ccgcttcttg ataaatcaca acatctccaa
    20761 gtggtgccaa gacaccccaa acaagggcat ttgcaagtag ttgaatgagg ttaaaaataa
    20821 gaatatcttt ccagtcaaaa acaccattga tcactcgaac gtactttcta aaaagtccca
    20881 caactaaacc aaagagtccg ctagcgataa tccaagtcca ccatagacca taaccagcaa
    20941 gagagtcctt gattgcatga ccaatcaacc cgacaagcaa accgataatc ggtccaaaaa
    21001 taatagaaag tagcgcttgt accgcatact gaagctggat gcttgtattt ggaacagggg
    21061 ttggaatgtt gatcatcccg atgacgacaa agagggcagc gccaattccg acagcaacaa
    21121 cttgtttaat tgtaaatttg atttccatac tattctccta ttttatcctt ctattttctt
    21181 tatttcaatg gtccaagatg aaccgacacc tacattatag gccttggcaa aggaaccttg
    21241 gttgatagcc aaacctaaac gatagagaga gttgatgtaa aggatgggtt gcccaattct
    21301 cacatctgca aatgatttgc catagacaac ctgattttga tagaccagca tatcagcatg
    21361 atagatggtc acttcaaaac gatcaccaaa ttctggttcc agcttgtaaa attcttcccg
    21421 tgtgatagag gtccaaagcg aaccgaaacg cacatccaga atatcaatgg ctcccttcac
    21481 cagatgatct tctatgatgg tcgctacgac tggaagctct acaatctggt ccacactgag
    21541 ctctggccct acttcctcaa aagtaatgtg accactggcc agtttagcac cagtataggc
    21601 atagacatca cgaccgtgga aggtataaga atgctctgtg ttttgacgcc tattggccac
    21661 ctcagaaatc tcacgaatgg ctacaatgcc aacgtgtttc ttgataaagg aaagcgtccc
    21721 attatctggc gtgacaatgt attgattttt tgcagtcttg gcaactacac tcttacgttt
    21781 cgaaccgaca cctggatcga caaccgatac aaacgtcgtt ccctcaggcc agtaatccac
    21841 cgtctgaaag agacgatagc tcccctcaaa aatattataa ggcgtgatat cgtgcgtcaa
    21901 gtgatggatt tttaaggttg gagactcttc taaagccact ccaatcatag ccgataccgc
    21961 accatcaacc agaccaaagt ctgattgtaa taccagtaaa ttattattca ttttatctcc
    22021 ttgtatatcc tttatcatac catatttcag agaaagtcgc taatagctat ttcaattggt
    22081 tagggtatag ttttacctta tagcgaattt tcactaaaag ctcttgttat tagctagtag
    22141 gtgcattttt tcttgaaaaa gggtatgata gttacatcat gaaaaagtag gttttattat
    22201 gaaaattatc cttgtcggag ggggaaaagt tggttttgcc ctctgtcgct ccttggttgc
    22261 agaaaagcat gatgttttgc tgattgagca agacgaagct gttctcaatc atattgtcag
    22321 tcgctttgat atcattggta tccttggtaa cggggccgat tttgccattc ttgagcaagc
    22381 cagcgtccaa gattgtgata tctttatcgc cctgactgag cacgatgaag tcaacatgat
    22441 tgcagcagtt ctagccaaga aaatgggagc taaagaaact atcgttcggg tgaggaaccc
    22501 tgaatattct aactcttatt tcaaggaaaa gaatattctc ggtttttctc ttatcgttaa
    22561 tcctgagctc ttggctgccc gcgctatcgc gaatatcatt gacttcccca acgccctgtc
    22621 tgtcgaacgc tttgctggtg gacgcgttag cctcatggaa tttgtcgtca agtccaccag
    22681 cggtctttgc caaatgccca tttctgattt tcgtaaaaaa tttggtaatg tcattgtctg
    22741 tgcgatagag agggatcatc aaattatcat tccaagcggt gacatgactg tacaggataa
    22801 agatagaatc tttgtcactg gtaaccgtgt cgatatgata ctcttccata attattttaa
    22861 atcacgcgcc gtgaagagcc ttctcatcgt tggagcaggt agaattgcct attatctact
    22921 tggtattctc aaagatagtc gtatcgatac aaaagtcatt gaaatcaatc ctgaaatcgc
    22981 cagcttcttt agcgagaaat tcccaaatct ctacatcgtt caaggagatg gaaccgcaaa
    23041 agatatcctg ctggaagaaa gtgctcaaca ctatgatgcc gttgcgactc taacaggagt
    23101 cgatgaggaa aatctgatta catctatgtt ccttgacagg gtaggtgtac agaaaaatat
    23161 tactaaggtc aatcgtacca gtctcctcga gattatcaat gcgcctgatt tttcaagtat
    23221 catcacacct aaaagcatcg ctgtagatac gattatgcac tttattcgtg gtcgagttaa
    23281 tgcccagtat tcagaccttc aagccatgca ccatctagcc aatggccaaa tcgaaaccct
    23341 gcaattccat atcaaggaag ccaataaaat gactgccaaa cctctttctc aactgaaatt
    23401 gaaaaaaggg gttcttattg cagccattat tcgaaagggc aagactattt tcccaactgg
    23461 ggaggatatg ttggaagttg gagacaagct cctagtaaca accttgttgc caaacatcac
    23521 caagatttat gacttgattg cgaggtaaga aatgaataaa agtatgattc gttacctcct
    23581 ttcaaagtta cttttgattg aagctgttct cctcttggtt cctgtggcta tcgctgtcta
    23641 ttaccgtgaa tcgagccaag tctttacagc cctcttttcg accataggga ttctcgtatt
    23701 gttaggcggt tcagggagtt tacagaagcc aaaaaatcaa cggatttatg ccaaggaggg
    23761 agtcttgatc gttgccctct gttggatcct ttggtctttc tttggtggtc tcccctttgt
    23821 cttttctggg caaatcccta gccttattga tgcctttttt gaaattagtt ctgggtttac
    23881 aactactgga gcaagtattt tgaacgacat ttcggttctc agccgttccc tcctcttctg
    23941 gcgaagtttt acccacttga ttggagggat gggagtgctt gtttttgcac ttgctattat
    24001 ggacaatgcc aaaaatagtc acctagaagt aatgaaggct gaggttcctg gtcctgtctt
    24061 tggtaaggtt gtatccaaat taaaaaacac tgcccagatc ctctatctcc tttacatagc
    24121 cctcttttcc ctctttgtca tcatctatta tctagccggt atgcctctct acgatagttt
    24181 tgtcattgct atggggacag cgggaactgg aggatttacc gtctataacg atggaattgc
    24241 ccactatggt agctcactga ttacctatct ggttagtata ggagttttgg tttttggggt
    24301 aaatttcaat ctctactact accttatgct ccgtcgggtt aaggccttct ttggagatga
    24361 agagcttcgg gcttacttgg tcatcgtgct ggtttctaca ggcttgatta gcctcaatac
    24421 tctctacctc tacccagggt tttcaaagag ctttgaaatg accttcttcc aggtttccaa
    24481 catcattaca acaactggtt ttggatatgg agatattacc aactggcccc tcttctccca
    24541 gtttatcctc cttttcctca tggcaatcgg tggctctgct ggatcaactg caggtggact
    24601 caagattatt cgaggcctca tcctttcaaa aattgccaaa aaccaaattt tgtcaatcct
    24661 atcgccccac cgtgttttga ccctccatgt taataaaatg gtgattgaca aagataccca
    24721 gcataagatt ctcaagtact ttgtcatcta tgctatgatt ttgctagcgc ttatctttat
    24781 tgtcagccta gatagcaatg attttctggt cgtgaccagc gctgtcttta gctgtttcaa
    24841 taatatcggg cctattctag gaaccacttc tagtttctca atctttagtc ccatctcaaa
    24901 aattctcctc tcctttgcaa tgattgcagg gcgcttggag atttacccaa tcctccttct
    24961 ctttatgaaa agaacttggt ctaagagata aaatacaaaa tccctctttt tacaattgtg
    25021 aaagagggat ttttatatta aaaaggggga acgtttttaa atccaagcct gtctgtaagc
    25081 aaagttctta ctcaaataaa agatggaaaa acttactaca cagcttggta atgctccaat
    25141 acaaattgta aggctttact tggattcttt gttaaatgaa tatattccat tcctctggta
    25201 gcaactatct gaatacctaa tctttctgca tccaccttca tctctctata ataactccga
    25261 acttgtggag ccagaataat taaatcataa acacccataa tatcataatg agctccgtac
    25321 gctcctgaat tcgcaatcac tctaacttct gctaattgag cgccctcgtt aattgcattg
    25381 gctaattacg cacttgttcc agaccctgca caaagaacca gtactttcaa ctccttactt
    25441 tttattacat ctatctcacc aggcattatt tcacttgtat cctcctctaa aataatatca
    25501 tttgagcttg caatatctat aacctgtagt aaatatctcg tatttagaca atatgaaaac
    25561 aagacgactt ccatatagga aaccgccttc tcgctatgtt gagtgattta tattaaaata
    25621 acttttcttc tagctgcatt ttattattat aaaaacattc atcataaccc ccagaactta
    25681 aataacaatt tttattcaag atacatactc ctagaataaa ctttatatga aattctcatt
    25741 tttgttttta caattctcct tagttaaatc ttgtttaata tatgttttac atatagtatt
    25801 tagcgcgaca taatactgaa gtctctctcc aaaaaacggt tatgcttctt tgaataggac
    25861 gttctcacaa gaaaagcatc ttcacgtttc aacttcatat ggctcaaaaa caatcaattg
    25921 atgctaaaac ctgtacctag atgtttcggt tcataaaacc atgaaactgt aaaagtggat
    25981 gaaattgata gcgatagtca aatcaagagg catcataact ctaaaaagtc acaatatata
    26041 agttcatcct cggaaaaata tcattctaat tgttgaaatg gatatataaa caggggtatt
    26101 aacattaaat gctcatgaaa caacgaatac aggtatcaaa actatgacaa aacaagtccc
    26161 taaatttact aaagacactg ctcaacttta cacctgtaaa tggttgttgt ataataaagt
    26221 tacaaagatg tacgaccaca ctgttgtaaa tcatagtgtt cgcgaatata ttactgatag
    26281 catttctaca aatacaattg aagaggctgg ataggcttta aacgaaatat gtcagtgttt
    26341 tggcattcct agccttaata tcatttaaag aattgtatag ataaaatttt ttccaataca
    26401 gagactcgta acaactgctt catttttcta ccaacatatt taggaacagg ataagataca
    26461 agaggattaa tccataaatc agttctatac caatctaaga taaataagct aaaaaacaat
    26521 tgataataag caaatagatt ccaaattttc tctatctgct cattttaata aacaatacta
    26581 gtgtaactat ccttccagtc agaagcttgt caaatcacac cgaaaattct tctaaaattt
    26641 atctcgttag gcaatcaagc aaaaactcga cgatagtaca aacattatca tacaggattg
    26701 acttcctaaa ttatatactt tagtaaggtt ttcgtataag aaaaaagatt cattttacat
    26761 ttctaaacat tcttttctaa gatgaaaaac agaatttttc gattgtgatt taaagcaaca
    26821 agaagatttt cagtatcatc ctatagatac gagctaatta agaaaaacta cattttcgaa
    26881 tataaactac aataatataa actaaatttt ataggaggaa gacaatggat tggtacgatt
    26941 atatgataca ggcatccaaa caatcacaat tcaacgcaag ccattggttt cgctatttgc
    27001 gaaaagttat ttttgaagac tattcttatt taacaaacca agatgtagaa aagttgctag
    27061 actccaaaga actaacccgt tttcaaaaaa ttagcttgaa gtatgccttt caagagcata
    27121 ctccaactca taaatatgtg atttcattaa ataaacctgc taagttaacc aatgttcaaa
    27181 aattgatgga gaaatacaaa catggataaa atgaaaccgg tcttccaagc cctaaataag
    27241 gaattaattc aggaaaatct gactttaaca attatctgtg tcgatggtta tgtcttagaa
    27301 tatcatggtt tacgtgccac acaagatgtt gatgcttttt atgaccaaaa tcagaaaatc
    27361 aatgaaatta ttgctcgtgt aggaaaacag tttaatctta acatccacga agagttatgg
    27421 ctcaacaatc atgttgcaaa gcaaatatga acaaacaacc tcccttaagt ttatgcgaat
    27481 ccttgtactc atttgagaat ctgaccgttt tagtagtccc tattgagtat gtgttaggaa
    27541 tgaagatgat gagtatcaga gagcaggatt tgcaagatat tggtgcaatt attaaataca
    27601 aaaactttca ttcaccattc gataccttta aatatttaaa agacatggga tttgatacta
    27661 ttgacctctc tgttctatta gaaggtttta gttatgctta tggtatggat tggttagaaa
    27721 aattttttaa agaaaatcaa gataaacttc gcgaatttta ttaacagtat caaacaagat
    27781 gattgtcaat tagccagtcg tcttgttttt tatgatgaaa tgaaataaaa tctgaacaaa
    27841 tcgcttagag aattccagtc aatttctagc gctgtttgag acgtttcagt ggactatagt
    27901 aaaatgaaat aagaatagga caaatcgatc aggacagtca aatcgatttc taagaatgtt
    27961 ttagaaataa aggtgtacta ttctagtttc aatctattat atttcagatt cggtccacta
    28021 ttccttccca aaaacattgc atattgcgcc gaaggaattg ctcatactat cccaagcaca
    28081 aaaccctcag tcgtttgacc gagggttcct ttttattatt caagaacttg attagctcaa
    28141 tgcatccaag gcgtcatcca ttgaaagaac ttcgtggaag acacgttgtg tcaattcagt
    28201 tttttgttct ggagtgaggt atttagtgtt tacacagtat ccagagatac gtacgataac
    28261 gtcttcgcct gacatgattt tttcgtaaac atcgttcaag tccataacgt tcaagttaac
    28321 gtgttgtcca ccgttttcga agtaaccatc aaggattgtt accaagttat caacttgttc
    28381 atcacgagtc ttaccaagag cgcgaggtga tacttgtgta gtcaatgaga taccgtcagc
    28441 tgcataacta aagtcaaggc tagaaagtga gttcaagttt tgcaaccaac cacctttagc
    28501 tttgttagat gggttagcac ctggtgagaa gaattcaagt ttagacaagt tcacagaacc
    28561 atcttcgttg aggtatacac ctttgtgaac tggtgagtta ccagtttgtt tagagtaagc
    28621 aacgttagat gtgattgtca aaagtgatac tgtagcttct gcgtctttgt atagtttgtg
    28681 gctacgtaga cgagttgtgt aagcttcgat caaccattct gccaattcgt ttgaacgtgg
    28741 gtcatcttca ccccagcgtg ggtagtcacc gattgtttcg taatcgtaga tgtagccatc
    28801 ttcgtcacgg attggtttaa ctgtagcgta tttgatagct gacaatgtat caacagtgtt
    28861 agcaaatcca cagataccga atcccatgtt ggcacgttgt ttagttggca agaaggccat
    28921 ttgaacagct tcgtagttgt acctatcagt catgtagtgg atgatgttca aggcatctac
    28981 gtaagtgtca gtcaaccagt caagagattt ttcaaagttc gctttaactg attcgaattc
    29041 aagaacttcg tcacggattg gttcgatatc aaatactttg tagtctttgt gaacatcgtc
    29101 gtaaccacca ttcaaaccag taagaagggc tttaagaacg tttacacgag caccgaagta
    29161 ctggatgttg tggcgttgtt cttcattttc tggatcaagt ggagacacac agcatgagat
    29221 acagctcatt tcaccatatc cgtctttagc cattgttgtt acaccttcgt attggataga
    29281 agagtgtttg tggctcatgt gcatacagta gcgacggaag ttgtatggca atttgtcagt
    29341 ccaaagaact gtcaagtttg gttctggtga gttaccgatg ttgtcaagag tgttcaagaa
    29401 acggtagtcc atcttagtaa cacggtgacg accgtcgtta cccataccag ccatagaagt
    29461 tgtgataaag gttgggtcac ctgagtacaa ttggtcataa gcttttgtac gagcaaattt
    29521 aactgtacga agtttcataa cgaaatcatc aacgaattct tggatttctg attcagtaaa
    29581 tgtaccacga gcaaggtcac gttctgcaaa gatgtccaat acgattggta cacgacctag
    29641 agatgtagca gcaccgttaa tcacacggca gacagccatg aaagcaatgt taacccattg
    29701 gattgcttct ttcacgttca tcgctggttt gcgaacatca accccgtaaa ggtcacccag
    29761 gcgaacaact tgttgcaatg cttggtattg aaggtttact tcttcacgaa gacggattgt
    29821 ttcttcatcg atttctttga ttgcattcca gtcgtttact ttttcttgca tcaagtagtc
    29881 tgcaccgtaa agagcaagac gtgcgtaaac accgatgata cgtccgcgtg agtatgcatc
    29941 tggaagacca gttacagtgt gtgcatgacg agcgcgacga atatttgaag tgtaggcacg
    30001 gaaaataccg tcgttaactg ttgttacata tttagtgaag atttcgtgaa cagctgggtc
    30061 tggttcgtat ccattttctt ttaaagtagt ttcagccata cggataccac cttttggcat
    30121 gaagttcaat ttgaagagtt catcattttg gataccaaag ataacttcat tttctttgtc
    30181 gataaatcca gcagggatat cagcgataga tgttggacga gtgtccattg ggaaacgagt
    30241 ttcttcgtag tgtgctttag tttcttctac aattttcttg atgtgaagtg aacgctctgt
    30301 tggtcctgca aggaagcttt cgtctccatc ataaggtgtg tagttagctt gtacaaagcg
    30361 tgatacactt gctttttctt tccaatctac gcctttgaag ccttcccaag ctttgtcaaa
    30421 aatatcttgt gcttcaacaa ctgtcttaac aaccatatta atgtcctctt ttttctagta
    30481 acagtcatct gttacattca taagacaagt ataccataaa gtaaccgatt tcaacaagtg
    30541 caaaatccct atttttacac tttcttttct aaaacagtct atattttatt tcaaactgta
    30601 ttatattttt gaaaaaaata aagtcctttt ttcttttttt cagaaaaaag ggtataataa
    30661 aagaaaataa gcagtaacac tcaatggaaa tcgaaaagca aactaggaag ctagccgcag
    30721 attgctcaaa acactgtttt gaggttgcag atagagctga cgtggtttga agagattttc
    30781 gaagagtata aaaaggtgct aggcatgttg atttttcctt tgttaaatga tttgtcaaga
    30841 aaaatcatcc atattgacat ggatgccttt tttgctgcgg tggaaatcaa ggataatcct
    30901 aaactcagag gaaaacctgt cattattgga agcgaccctc ggcaaacagg tggacgggga
    30961 gtcgtttcta cctgtagcta tgaggctcga gcttttggtg tccattctgc catgagttcc
    31021 aaggaagctt atgaacgttg tccccaggcc gtctttatct caggaaatta tgagaaatac
    31081 aaagctgtgg gactccagat tcgagctatt tttaagcgct atacagattt gattgaaccc
    31141 atgagcattg acgaagccta tttggatgtg acagaaaata aactcggtat caagtcagcg
    31201 gtcaaaattg ctcgcctcat tcaaaaagat atctggcaag aactccatct aactgcttct
    31261 gccggtattt cctacaacaa gttcttagct aaaatggcga gtgattatca aaaacctcat
    31321 ggtttgacag tgattctacc tgaacaggct gagaattttc tcaaacaaat ggatatttcc
    31381 aaatttcatg gagtaggaaa aaagacagta gaacgtcttc atcaaatggg cgtttttact
    31441 ggtgctgatt tacttgaagt tcctgaggtg accctaatag accgttttgg tagactaggc
    31501 tatgatctgt atcgaaaggc tcgtggcatt cacaactctc cagtcaaatc caatcgcatc
    31561 cgtaaatcaa tcggcaagga gaaaacctac gggaagattc tccgtgctga ggaagatatc
    31621 aaaaaagagc tgactcttct atcagaaaaa gtcgctctca atctacatca acaagaaaaa
    31681 gctggaaaaa ttgtcatttt gaaaatccgc tacgaggact tttcaactct taccaaacga
    31741 aaaagtcttg ctcaaaaaac acaagatgct agtcagataa gccaaatagc cctgcaactc
    31801 tatgaagaat taagcgagaa agaaagaggt gtccgcctat tggggattac cctgactgga
    31861 ttttaaagct caatgaaaat caaagagcaa actaggaagc tagccgcagg ctgctcaaaa
    31921 cactgttttg aggttgcaga taaagctgac gcggtttgaa gagattttct aagagtataa
    31981 aaccttgaag agttgcccct tcaaggtttt tcttatacaa ataaacggac aaaactgtaa
    32041 agtagcaaaa cactaccaag tacgatacgg tatttaccaa aaagggtgaa gtcgtgtttt
    32101 ttcacatagc tggtcaagaa gcgaatagcc accatgctga ccgcaaaagc tactcccatc
    32161 gcgaccaaga gcaaaaacaa ttgcccaaag ctcaagagtt ctccggcttt cacaaatttg
    32221 aaaatcttta aggcactagc tccaaacata acgggaatcc caagatagaa ggtaaattct
    32281 gtcacaactg aacgactggt tccatttaac aaaccaccga caatcgttgc acctgaacgg
    32341 ctagtccctg gtaaaagagc aagaacttgg aagagtccga tatagaaagc ggtcgtataa
    32401 ggaagcttgt ccaactctgt tacacttggc tcgatagcac gcgctttatt gcgcttttcc
    32461 aaatagatga aggcaacccc gtagataatc aacatgagag caactgaaac catgttatgg
    32521 aagtgggtat caaaccaatc atcaaattta aagacaccaa gtaaaggtaa agtagcaatc
    32581 aagaccttca accatagtct ccaagtctta cgaacttcct gtttgtcctt agtcggttta
    32641 aaaggattga gcttgttaaa ataaatcacc ataactgcta aaatagcacc aagctgaatc
    32701 acgacattaa acatggacat aaaggcttca ttttgatttt ggtattggat gaattcctct
    32761 gctaaaatca agtgacctgt actggaaatc ggcaaccatt ccgtaattcc ttcaacaatc
    32821 ccgaagaaga tagattttaa aatttcaata agatacatag attactccct atattataat
    32881 tagcgcagtt agctactgcc aaagcccaag tggtatactt ggaataagcc actgtggatt
    32941 agttcatttc ctccaatcgc ttgacgattt ccgaaaggat ctaaccacag
//
LOCUS       DBEROU010000025        28929 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.025, whole genome shotgun sequence.
ACCESSION   DBEROU010000025 DBEROU010000000
VERSION     DBEROU010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 28929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28929
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.025"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(121..684)
                     /locus_tag="ACL8CL_001207"
     CDS             complement(121..684)
                     /locus_tag="ACL8CL_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HGS1330458.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            complement(668..1219)
                     /gene="coaC"
                     /locus_tag="ACL8CL_001208"
     CDS             complement(668..1219)
                     /gene="coaC"
                     /locus_tag="ACL8CL_001208"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HGS1330459.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            complement(1231..1920)
                     /gene="coaB"
                     /locus_tag="ACL8CL_001209"
     CDS             complement(1231..1920)
                     /gene="coaB"
                     /locus_tag="ACL8CL_001209"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HGS1330460.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2150..3820
                     /locus_tag="ACL8CL_001210"
     CDS             2150..3820
                     /locus_tag="ACL8CL_001210"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HGS1330461.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAVL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            4148..5323
                     /gene="mutY"
                     /locus_tag="ACL8CL_001211"
     CDS             4148..5323
                     /gene="mutY"
                     /locus_tag="ACL8CL_001211"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HGS1330462.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNFGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            5379..6083
                     /gene="yycF"
                     /locus_tag="ACL8CL_001212"
     CDS             5379..6083
                     /gene="yycF"
                     /locus_tag="ACL8CL_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator YycF"
                     /protein_id="HGS1330463.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            6076..7425
                     /gene="vicK"
                     /locus_tag="ACL8CL_001213"
     CDS             6076..7425
                     /gene="vicK"
                     /locus_tag="ACL8CL_001213"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HGS1330464.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQGGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            7427..8236
                     /locus_tag="ACL8CL_001214"
     CDS             7427..8236
                     /locus_tag="ACL8CL_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HGS1330465.1"
                     /translation="MSKIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            8327..8569
                     /gene="relB"
                     /locus_tag="ACL8CL_001215"
     CDS             8327..8569
                     /gene="relB"
                     /locus_tag="ACL8CL_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB family
                     antitoxin"
                     /protein_id="HGS1330466.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            8571..8825
                     /locus_tag="ACL8CL_001216"
     CDS             8571..8825
                     /locus_tag="ACL8CL_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGS1330467.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQTNVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            8872..9615
                     /locus_tag="ACL8CL_001217"
     CDS             8872..9615
                     /locus_tag="ACL8CL_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HGS1330468.1"
                     /translation="MNKKWLFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFTTKRKWRFILENIFDLLAILPLNAIFTVFRLGRIFRLAKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLIYILYVNIFIVLVGSSILSVVEEKSFS
                     NSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVGIGTIGMLTSALTNFFVKDNPDE
                     QIKLDKLKDELSSQRILLEKQSEKIEELH"
     gene            complement(9916..10902)
                     /locus_tag="ACL8CL_001218"
     CDS             complement(9916..10902)
                     /locus_tag="ACL8CL_001218"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HGS1330469.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            11096..13564
                     /gene="gyrA"
                     /locus_tag="ACL8CL_001219"
     CDS             11096..13564
                     /gene="gyrA"
                     /locus_tag="ACL8CL_001219"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HGS1330470.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMVGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLVITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            13564..14307
                     /gene="srtA"
                     /locus_tag="ACL8CL_001220"
     CDS             13564..14307
                     /gene="srtA"
                     /locus_tag="ACL8CL_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HGS1330471.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSNEILTAFNQPYKQFY"
     gene            14551..15348
                     /locus_tag="ACL8CL_001221"
     CDS             14551..15348
                     /locus_tag="ACL8CL_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HGS1330472.1"
                     /translation="MISSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            15496..16755
                     /locus_tag="ACL8CL_001222"
                     /pseudo
     CDS             15496..16755
                     /locus_tag="ACL8CL_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365067.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
     gene            16851..18125
                     /locus_tag="ACL8CL_001223"
     CDS             16851..18125
                     /locus_tag="ACL8CL_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HGS1330473.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRQEKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            18135..19013
                     /gene="truB"
                     /locus_tag="ACL8CL_001224"
     CDS             18135..19013
                     /gene="truB"
                     /locus_tag="ACL8CL_001224"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HGS1330474.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEITLGYSTTTEDASGEVIAETPVLSPLYEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALSLEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            19254..19556
                     /locus_tag="ACL8CL_001225"
     CDS             19254..19556
                     /locus_tag="ACL8CL_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HGS1330475.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQS
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            19664..20302
                     /gene="udk"
                     /locus_tag="ACL8CL_001226"
     CDS             19664..20302
                     /gene="udk"
                     /locus_tag="ACL8CL_001226"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HGS1330476.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            20430..21770
                     /gene="xseA"
                     /locus_tag="ACL8CL_001227"
     CDS             20430..21770
                     /gene="xseA"
                     /locus_tag="ACL8CL_001227"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HGS1330477.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKAEPDG
                     VGALAIQFEQLKKKLTEEDLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMATAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEAKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            21748..21960
                     /locus_tag="ACL8CL_001228"
     CDS             21748..21960
                     /locus_tag="ACL8CL_001228"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HGS1330478.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            21957..22832
                     /locus_tag="ACL8CL_001229"
     CDS             21957..22832
                     /locus_tag="ACL8CL_001229"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HGS1330479.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVAIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDPYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQIPFE
                     TESIVSVVESLRING"
     gene            22825..23640
                     /locus_tag="ACL8CL_001230"
     CDS             22825..23640
                     /locus_tag="ACL8CL_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HGS1330480.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKVHQNVLESVTAMAVEV
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            23633..24064
                     /locus_tag="ACL8CL_001231"
     CDS             23633..24064
                     /locus_tag="ACL8CL_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS1330481.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            24071..25738
                     /gene="recN"
                     /locus_tag="ACL8CL_001232"
     CDS             24071..25738
                     /gene="recN"
                     /locus_tag="ACL8CL_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HGS1330482.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDKQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSYLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNKMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            25740..26468
                     /locus_tag="ACL8CL_001233"
     CDS             25740..26468
                     /locus_tag="ACL8CL_001233"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HGS1330483.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQPQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYSRNGGDTTINSILGRPLD
                     APVDGVEDAKRVAAEASDLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            26547..28370
                     /gene="lepA"
                     /locus_tag="ACL8CL_001234"
     CDS             26547..28370
                     /gene="lepA"
                     /locus_tag="ACL8CL_001234"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HGS1330484.1"
                     /translation="MNLEELKKRQGKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMDLERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
     gene            complement(28451..28783)
                     /locus_tag="ACL8CL_001235"
     CDS             complement(28451..28783)
                     /locus_tag="ACL8CL_001235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330485.1"
                     /translation="MKIKEQTRKLAAGYSKHSFEVVDKTDEVSSKHDFEVVDETDEVS
                     SKHDFEVVDETDEVSSKHGSEVVDETDEVSSKHDFEVVDETDEVSNHTYGKATLTWFE
                     EIFEEYKN"
BASE COUNT         8715 a         5146 c         6624 g         8444 t
ORIGIN      
        1 tttgactgtc ctgatcgatt tgtcctgttc ttatttcgtt ttactatagt aaagatttca
       61 ttaaaaagaa actgtataga gcaaaatctc caccttcagg tttgaaagcg gagattgttt
      121 ttattttttc aaggtttgta gtcgtggaac aatggctagg gttagaattg cagaaatgac
      181 caattcagca attgaatttg ttgagataac ggttgccaga agaagttgga tatttccatt
      241 ataaacattt ccaaatagga agaagattcc tccaaggaca aagatagtat ttgtcaagga
      301 accaagggct ccagctaaaa tcagaccagt cttgtttttc atcagtttat agactaagta
      361 aggagttaaa ccaatcaaaa tacgtgggac gatggcaatg atagctgagt agatgtttcc
      421 gtttggtacg aagggagaga agaggtagct tgtcggtaga atcgtaatcg tgttaaccgt
      481 caagctaagt aatcccatca aaaatccaag tgtaacccca actcgtggac cataaataat
      541 gctggcaata atgacaggaa tatgaacaat ggtcggtttg attggaaatg gaaaaaggtt
      601 aaagataagt gagctcagaa agtgtatcac gagcatggta gcaaaaaaga tagcaatggg
      661 tgcaatatta gagcgttttt tcatcgatag tttcctttat tctttctaaa ataattgtga
      721 ggtcagctaa agctcctcgt ccgtggtctc cacaagctag tagagattcc ttaggagcaa
      781 tcagctgata gccgtaggtt tctaatgttt tcagattatt ctgagttact ggatggtcat
      841 acatttttgt attcatagca ggagctatta gtttgggaat atgacttggt agggctagag
      901 ctgtactggt taccatgttg tccgcaaatc cgtgagctag ttttgcaata gtgttagcag
      961 ttgcaggtac cacgataaat aaatctgctt tttttccaag ttcgatatga ttgacctgat
     1021 caggataggg ttccttcatg acatccaagt ggacaggatt ctgtgagagt acctgtagtg
     1081 tcaaaggttg gataaactct gtagcagcct gagtcattaa gacagtgact tgatggcctt
     1141 gtttttttag agaactgact aaatctgccg acttataaga ggcgattgaa cccgttacag
     1201 ccaagagaat gtttgccata gttttccttt ctaagaatga taggcttgaa ttttttcaag
     1261 gaggagttct gcaatttctt ctttagtctg gactgtttga agctgatttt tctcaacaaa
     1321 tatagctcgg tgctgacctg ctgaaatttg agtcaggtca ttcgcgatga ttaaatctgc
     1381 ttgattcttg ataagacttt ttcgtgcaat gtcaaccaga tgatcttcgg taacatcaac
     1441 cagcagtttg aaaccaatca gatgaatagt aggattccat tccttgacta gggatatgat
     1501 tttgggtgtc tttttaagga acaaaacctg aacctcatca gttgaagaaa tcttggcctg
     1561 atgattctgc ttgcttaaaa attcttttag attggagcta gcctgaactt cctcaagccc
     1621 tgtcatataa acaggagtgt agtcagaaac agccattgag tggatcaaga cctgataatc
     1681 ctgaacacgt tcttgcattt ctattagaag gtccttggta ttggtaattt ctcgaatact
     1741 taggttagga tgaggctctg gcttcagagc tcgttttgtc gtaattaaac aaacttcatg
     1801 ccctgcagaa agcaaagtct ctgtgataat tttccccaag tgacctgtag aatggttagt
     1861 gatagagcgg acgctatcga tagcttcact ggtaccgccc gatgtaacta aaattttcat
     1921 agcactattg tacacaaaaa taagcggtaa gtaaaatgaa agtgataaat ttttggtaaa
     1981 aacgaagatt tactatagtt tcaaactata aagccatata aaatttaaga aaggactcta
     2041 aaagccttaa aaacagagat atttacgaac gtttagagag ttttaggatg ttaaaaatct
     2101 tgttttgtga tataatgaat tatcatataa gaggttagag gaattttgaa tgaaaacaga
     2161 tattgaaatc gcacagagta ttgagttgaa gccaattgtt gatgttgtag agaaacttgg
     2221 tatttcttac gacgatttgg agttgtacgg aaagtacaag gctaaactca gctttgataa
     2281 aattcgtgca gttgagagca atccagtcgg taaattgatc ttggttactg ccatcaaccc
     2341 aacacctgca ggtgaaggaa agtcgacgct taccattggt cttgcggatg ccttgaacaa
     2401 gattggcaaa aaaaccatga ttgctatccg cgaaccgtct cttggtccag tcatggggat
     2461 caagggtggt gctgctggtg gtgggtatgc acaagttctg ccaatggaag acatcaacct
     2521 ccactttact ggagatatgc atgctattac aactgccaac aatgcccttt ctgccttgat
     2581 tgacaaccac ttgcaccaag ggaatgagct gggaattgat caacgtcgta tcctctggaa
     2641 acgcgttgtg gacttgaacg accgtgcgct ccgccatgtg actgttggtc ttggtggtcc
     2701 tctaaacggt attccacgtg aggacggttt tgatattaca gttgcttcag aaatcatggc
     2761 aattctttgc ttggcaacgg acatcgagga cttgaaacgt cgtttggcga atatcgttat
     2821 tggttatcgc tatgaccgta cgcctgtttc tgtaggtgat ttgcaggttg agggtgcctt
     2881 ggctttgatt ttgaaggatg ctattaagcc aaacttggtt cagacaattt acggtacacc
     2941 tgcctttgta cacggtggtc catttgccaa tatcgctcat ggctgtaact ctgttttggc
     3001 gacgacaaca gcccttcact tggctgatta cactgttact gaagctggtt ttggtgcgga
     3061 ccttggtgct gagaaattcc ttgatatcaa gacaccaaac ttgccaacat ctccagatgc
     3121 agttgttatt gtcgcaaccc ttcgtgccct taagatgaat ggtggtgtgg ctaaagacgc
     3181 tctgactgaa gaaaatgtag aggcagttcg tgcaggtttt gctaacttga aacgccacgt
     3241 tgaaaatatc cgtaagttcg gtattccagc agttgtagct attaacgaat ttgtatctga
     3301 tacagaagct gaaattgcag tcttaaaaga actctgtgcc tcaatcgatg taccagttga
     3361 attggctagt gtctgggctg atggagcaga aggtggagta gcacttgccg aaacagttgt
     3421 taagacaatt gctgaaaatc cagctaacta taaacgtttg tatgataacg acctttctgt
     3481 ccaagaaaag attgaaaaaa ttgtcactga aatctatcgt ggtagcaaag tgaactttga
     3541 gaagaaagct caaacacaaa ttgctcaaat cgttcaaaac ggttgggaca aattgccaat
     3601 ctgtatggct aaaactcaat acagtttctc agacaatcca aatgcgcttg gagcaccaga
     3661 aaactttgaa attaccattc gtgaattggt accaaaatta ggtgcaggct tcatcgttgc
     3721 cttaactggt gatgttatga ccatgccagg tcttccaaaa cgtccagcag ctctcaacat
     3781 ggatgttgaa agcgatggaa ctgtactagg cttgttctag tggattgaaa ttgactttaa
     3841 gtgagtttgg aaataatatc caagctcatt ttttctcaga ctgcaagcag ttcatcaata
     3901 agatttatac tcttcgaaaa tctcttcaaa ctacgtcagc ttcaccttgc cgtaggtata
     3961 tgatactgac tctgtcagtt ctatccacaa cctcaaaaca gtgttttgag caacctgcgg
     4021 ctagcttcct agtttgctct ttgattttca ttgagtataa gtagatagaa aagttatata
     4081 ctatctattg aaaatattat aaatcagata caaagacagg tgaaaccttc tgtgttatac
     4141 tagagatatg ttagatttga aagaatacgg tatcgtcatg tggccggagg agaaagtcat
     4201 ttctttccgt gagaaacttc tcgcttggta tgatgaaaac aaaagagatt tgccttggag
     4261 gagaagtaaa aatccttatc acatctgggt atctgaaatc atgcttcagc agaccagggt
     4321 ggatacagtt atcccttact acgaaagatt tttggactgg tttccaactg tcgaaagtct
     4381 ggcaactgcg cctgaggaga gtttactgaa agcttgggag ggcttgggct attattctcg
     4441 agttcgcaat atgcaggctg cagcccagca gattatgact gactttggtg gccaatttcc
     4501 aaatacctat gaaggaattt ccagcttgaa agggattgga ccttacacag caggagccat
     4561 ttccagtatt gcttttaact tgcctgagcc agctgtagat ggtaatgtca tgcgggtctt
     4621 ggcgcgtctg tttgaagtca accacgatat tgggattcca agtaatcgaa aaatttttca
     4681 ggcaatgatg gagatcttga ttaatccgga tcgtccgggt gattttaatc aagcattgat
     4741 ggacttaggg tctgatattg agtctcctgt aaatcccaga cctgaagaaa gcccagtcaa
     4801 ggactttagt gcggcatatc agaatggaac catggaccgt tatccaatta aatctcctaa
     4861 gaaaaagccg gttcctattt atcttaaagc cttagttgtg aaaaattctc aaggacaatt
     4921 tttacttgaa aaaaatgaaa gcgaaaagct cttggcaggt ttttggcatt tcccctttat
     4981 agaagttgat aacttttcgc aagaagagca gtttgacctc tttcatcagg ttgcggaaga
     5041 aagtgtaaac tttggtccca gtccagaaga gagtttccag caggactatg acctagatgt
     5101 tgattggctt gatgtttgtt ttgatacagt ccagcatgtt tttagtcatc gaaaatggca
     5161 tgtacagatt gtagcaggtc aggtgagtga cttccatgat ttttcagata gggaagttcg
     5221 ttggctttca ccagaagaat tcaagaatta cccacttgcc aaaccccaac aaaaaatctg
     5281 gcaggcttat gcacaagcca acttagacag tagccaagac tagctattgt gtcttctgac
     5341 tatttttttg gtataatagt agagaaaaag gtgaacatat gaaaaaaata ctaattgtag
     5401 atgatgagaa accaatctcg gatattatca agtttaatat gaccaaggaa ggttacgaag
     5461 ttgtaactgc ttttaatggt cgtgaagcgc tagagcaatt tgaagcagag caaccagata
     5521 ttattattct ggatttgatg cttccagaaa ttgatggttt agaagttgct aagaccattc
     5581 gtaagacaag cagtgtgccc attcttatgc tttcagccaa agatagtgaa tttgataagg
     5641 ttatcggttt ggaacttggg gcagatgact atgtaacgaa acccttctcc aatcgtgagt
     5701 tgcaggcgcg tgttaaagct cttctgcgtc gttctcaacc tatgccagta gatggtcagg
     5761 aagcagatag taaacctcaa cctatccaaa ttggggattt agaaattgtt ccagacgcct
     5821 acgtggctaa aaaatatggc gaagaactag acttaaccca tcgtgaattt gagcttttgt
     5881 atcatttagc atcgcataca ggtcaagtca tcacgcgcga acacttgctt gagactgtct
     5941 ggggttatga ctattttggt gatgtccgta cagttgatgt gactgtacga cgtctgcgtg
     6001 agaagattga agatacgccc agccgaccag agtatatctt gacgcgccgt ggtgtagggt
     6061 attacatgag aaataatgct tgatttactg aaacaaacca tttttaccag agattttatc
     6121 tttatcctga ttttgttagg tttcatcctt gttgtgaccc tcttattact ggaaaataga
     6181 cgtgataata ttcagttgaa gcaaatcaat caaaaggtta aagatttgat tgcaggagat
     6241 tattccaagg ttcttgatat gcaaggtggg tctgaaatca ccaatattac caataatttg
     6301 aatgacttgt cggaggttat tcgtctcact caggaaaatc tagaacaaga gagtaagagg
     6361 ctaaatagta ttctgtttta tatgacagat ggggttcttg cgaccaaccg tcggggtcag
     6421 attatcatga ttaacgatac agccaagaag caactggggt tggttaagga agatgttctg
     6481 aatagaagca ttttggaatt gctcaagata gaagaaaact atgaattgcg tgatttgatt
     6541 acccaaagtc cagaattgtt gctagattcc caagatatca atggtgaata tttgaacctt
     6601 cgagttcgct ttgccttgat acgtcgagag tctggcttta tttcaggttt ggtggctgtt
     6661 ttgcatgata cgacggagca ggagaaggaa gaacgcgaac gaagactctt tgtttccaat
     6721 gttagccatg agttacggac tcctctgact agcgtaaaat cctatcttga agccttggat
     6781 gagggggctt tgtgtgaaac tgtagcacca gactttatca aggtttctct tgatgagacc
     6841 aaccgtatga tgcgcatggt gacggatctc ctccatcttt cacgtattga taatgctacc
     6901 agtcacctag atgtggaact gattaacttc actgctttta ttacctttat cctcaatcgt
     6961 tttgacaaga tgaaaggaca ggaaaaggag aaaaaatatg agttggtgag agattatccc
     7021 atcaattcta tctggatgga aattgataca gataagatga cgcaggttgt cgacaatatt
     7081 ttaaataatg ctattaagta ttcgccagat gggggtaaaa tcactgtcag aatgaagaca
     7141 actgaagacc agatgatttt atccatttct gaccacggtt tggggattcc taagcaggat
     7201 ttaccacgta tctttgaccg tttctatcgt gtggatcgtg ctagaagtcg tgcacaaggt
     7261 ggtacaggtc taggactgtc tatcgctaaa gaaattatta aacaacataa gggctttatt
     7321 tgggccaaga gtgaatacgg caagggttca acctttacca ttgtgctccc ttatgataag
     7381 gatgcagtga aagaagaagt atgggaggat gaagtagaag actagaatga gtaaaatagg
     7441 ctttaaatac agtattttag cgtcgggttc cagtggaaat tctttttatc tggaaacctc
     7501 aaaaaagaag cttttagtgg atgcaggctt gtctggcaag aaaattacca gtctgctagc
     7561 tgaaattaac cgcaagccag aagacctgga tgccatcttg attacccatg agcattcaga
     7621 tcatatccat ggagtaggcg ttttggctcg caagtatggt atggatcttt atgccaatga
     7681 aaagacctgg caagctatgg aaaatagtaa atatcttggc aaggtggatt cttcgcaaaa
     7741 gcatattttt gaaatgggta aaaccaaaac ttttggagat atcgacatcg agagttttgg
     7801 tgtaagccat gatgcagtcg caccgcagtt ctatcgcttt atgaaggatg acaagagttt
     7861 tgtccttttg acagatacag gttatgtcag tgaccgtatg gcgggcattg tcgaaaatgc
     7921 ggatggctat cttatcgagg ccaaccatga tgtagagatt ttgcgatcag gttcttacgc
     7981 ttggcgactc aaacaacgaa tcctatctga ccttggtcac ctttctaacg aggacggtgc
     8041 tgaagctatg attcggacgc taggaaatcg tactaagaag atttaccttg ggcatttatc
     8101 taaggaaaac aatatcaagg aactggctca tatgaccatg gtcaatcagc tggctcaagc
     8161 tgatctggga gtcggagtag actttaaggt ttatgatacc tcaccagata ccgcaacacc
     8221 attgacagag atatagaaag aacgctgaga aggtgttctt tttatattga ctgaacacct
     8281 aaaaagtaat acaatggtgt taccattaaa aaagggagca caaaagatga ctactattac
     8341 attaaaagtt tctgaagctg ataaaacatt tatgaaagca atggctaagt ttgaaggagt
     8401 ttccctttcg gaacttattc gcaccaaaac tcttgaagcc ctagaagatg aatacgatgc
     8461 tcgtgtggca gatttagcct atcaagagta tttagaagac ttggaaaaag gagttgaacc
     8521 cattacttgg gaagaaatga tgcatgattt aggcttgaag gatgaataat ttgtataaat
     8581 tagttccaac aagacgtttc atcaagcaat tgaaaaaatt ggaccgttat acgcagaagc
     8641 taattacaaa ctatttacaa accaatgttt tggaagaccc aagacgacac ggaaaggctt
     8701 tggttggtaa tcgcgttggt caatggcgct atagaattgg taattatcga gttatcgtac
     8761 aaattgtaga tgatgaatta gtcgttgcta ctctagaagt tggtcatcgg agagatattt
     8821 attgaattaa agctgagtaa taacagtata gaaagtaagg atagtaaaaa tttgaataaa
     8881 aaatggttat ttgcagatta ttatgataca acaattattt tacttgcttt aatctctgtt
     8941 attctggtat tacttggatt tgctgagatg attgatttgg acaatccgcc ttatagtatt
     9001 attgatttag taatctgggg tgtttttgta attgattata gttggcgctt ttttacgact
     9061 aaaagaaaat ggcgctttat tcttgaaaat atttttgatt tattggctat tctcccttta
     9121 aatgctattt ttacagtgtt tcgattaggg cgtatctttc gtttagctaa actaacaaaa
     9181 ttactgaaac tgactcgttt acttagaata attggactta caggtaaatt agaaagaaaa
     9241 attagtagat tcttacggac aaatggcctg atttatatct tgtatgttaa tatctttatt
     9301 gttttagtag ggagttcgat tttatccgtt gttgaagaaa aatccttctc aaatagtctt
     9361 tggtgggctc ttgtgacagt aactactgtt ggatatgggg atattgttcc tgcttccatt
     9421 tttgggaaat ggctagcagt tttactgatg cttgtcggta ttggaacaat agggatgtta
     9481 acaagtgctt tgacgaactt ttttgtaaag gataatccag atgaacagat aaaacttgat
     9541 aaactcaaag atgaattatc gagccaaaga atattactag agaaacaatc tgaaaaaatc
     9601 gaagaactac attgaatgat aaaagattta gttgaaaaaa tatcataaag gtgagacaaa
     9661 acaaaacttg attcgaggag aaatcaagta agtcttgaca caatagaacg catattatca
     9721 ggtataaaaa cttgatacta tgcgttttat agagaaataa aataaaatct gaacaaatca
     9781 attaggaatt taaagcattt tataacaacg ttttagagtg atagggtgct atttcaactt
     9841 caacctacta taatacagaa aaaaatacag gaaaaaacaa ctccctgatg attcaaggag
     9901 ttgtctatag ttaaattagt ttttagaagc ttcttggaat tctggatttt tccatgcttc
     9961 gtcaataata gcttgcaatt ctttagcaga tgcttgcatt ttttgagttt ctgcatcgtt
    10021 caatggaatg tttactggac gaacgatacc atgtgcacca acaacagctg gttgaccgat
    10081 aaagacattc tcaactccgt attgaccttc ttggaatact gaaagtggaa gtactgcgtt
    10141 ttcatcgtca aggattgctt tagtgatacg agcaagggct actgcgatac cgtagtatgt
    10201 tgcacctttt ttgttgatga ttgtgtaggc tgcatcacga acaccttcga acaattcaat
    10261 caattcagct tcttgaacat tttgagtgtc tttaagaaat tcttcaaggt ttacaccagc
    10321 gatgttagcg tgtgaccaaa cagcgaactc agagtcaccg tgttcaccca tgatgtaggc
    10381 gtgcactgaa cgagcatcca catccaattt ttcagcaagt gcttgacgga aacgagctga
    10441 gtcaagtgaa gtacctgaac cgataacgcg ttctttaggg aaaccagaga atttccaagt
    10501 tgagtaagtc aaaacgtcaa ctgggttagc agcaacaagg aagatacctt tgaaaccaga
    10561 ttcaacaact tgagttacga ttgatttgtt gatagcaagg tttttaccta caaggtcaag
    10621 acgagtttca cctggttttt gaggtgcacc tgcagtgatc acaacaaggt cagcgtctgc
    10681 acagtcagag tattgagctg catagatttt tttaggtgaa gtgaaggcaa gggcgtgact
    10741 aaggtcaagc gcatcaccaa cagctttttc atgcaattgt ggaatttcga taattccaag
    10801 ctcttgtgca attccttggt taacaagtgc aaaagcgtaa gatgaaccta cagcaccatc
    10861 accgacaagg ataacttttt tgtgttgttt agttgaagtc attgttttaa acatctcctt
    10921 aattttatta ggggattttc cctagacaac ttcattctat cacttttaaa aaactttgtc
    10981 acgaatatgc cttatagttc tcgatgtaaa cgttttagtg gtttagaggc tgaaatagat
    11041 gggaatttat ggtataatgt tgttacttac taattgtgaa atgaggcatt tattaatgca
    11101 ggataaaaat ttagtgaatg tcaatctgac aaaggagatg aaggcaagtt ttatcgacta
    11161 cgccatgagt gttatcgtag cgcgagctct tcctgatgtt cgagatggct taaaacctgt
    11221 tcaccgtcgc attctctacg gaatgaatga attgggtgtg accccagaca aaccccataa
    11281 aaaatctgct cgtattacag gggatgtcat gggtaaatat cacccacacg gggattcctc
    11341 tatttatgaa gccatggtcc gtatggctca atggtggagc taccgttaca tgcttgtaga
    11401 tggtcatggg aattttggtt ccatggatgg agatagtgct gccgctcaac gttataccga
    11461 ggcacgtatg agcaagattg ctctggaaat gcttcgtgat atcaacaaaa atacagttga
    11521 tttcgttgat aactatgatg ccaatgaacg ggaacccttg gtcttgccag cgcgttttcc
    11581 aaaccttttg gttaatggag caactggtat cgcggttggg atggcaacca atattccacc
    11641 tcataatctg ggtgaaacca ttgatgcagt gaagttggtc atggataatc ctgaagtgac
    11701 taccaaggac ttgatggaag tcttgcctgg accagatttt ccaactggtg ctcttgtcat
    11761 ggggaaatca ggtatccata aggcttatga aacaggtaaa ggttcgattg tcctacgttc
    11821 tcgtacagag attgaaacga ctaagactgg tcgtgagcgt atcgttgtaa cagaatttcc
    11881 ttacatggtc aataaaacca aggtgcatga gcatattgtt cgcttggttc aggaaaaacg
    11941 cattgagggt atcacagcag tacgtgatga gtcaaaccgt gaaggtgttc gatttgttat
    12001 tgaagtcaag cgcgacgcct cagccaatgt tattctcaat aacctcttca aaatgaccca
    12061 aatgcaaacc aattttggtt tcaatatgct cgctatccaa aatggtatac cgaaaatttt
    12121 gtctcttcgt cagattttgg atgcttatat cgagcaccaa aaagaagtgg ttgttcgtcg
    12181 tacacgtttt gataaggaaa aagcggaagc gcgcgctcat atcttagaag gtctcttgat
    12241 tgcgctagac catatcgacg aagtgattcg tatcatccgt gctagtgaaa cggatgcgga
    12301 agctcaagct gagttgatga gcaagtttaa gctttctgaa cgtcaaagtc aagctatcct
    12361 tgatatgcgt cttcgtcgtt tgacaggttt ggaacgcgat aagattcaat ctgagtatga
    12421 tgacctcttg gctctgattg cggatttagc agatattctt gctaagcctg aacgtgtttc
    12481 tcaaattatc aaagacgaat tggatgaagt taaacgtaaa ttttctgata aacgccgtac
    12541 agagttgatg gttggacagg tcttgagtct cgaggatgag gacttgattg aagaatcgga
    12601 tgtcttgatt accctttcta acagaggcta cattaagcgt ttggatcagg acgagttcac
    12661 tgctcaaaaa cgtgggggtc gtggtgtcca aggaacggga gtgaaagatg atgactttgt
    12721 tcgtgagtta gtgtcaacta gcacccatga tcatctgctc ttcttcacaa acaagggacg
    12781 tgtctatcgt cttaaaggtt atgaaattcc tgagtatggt cggactgcca aagggctacc
    12841 agtagtcaat ctcttgaaat tggatgaaga cgaaagtatt cagacggtta tcaatgttga
    12901 gtctgatcgc agtgatgatg cttatctctt ctttacaacc cgtcacggta ttgtgaagag
    12961 aaccagtgtt aaggagtttg ccaatattcg tcaaaatggt ctcaaagcgc tgaatttaaa
    13021 ggatgaagat gagttaatca atgtcttgtt ggcagaagga gatatggata ttatcattgg
    13081 taccaagttt ggttatgcag ttcgctttaa tcaatcagcc gttcgtggta tgagccgtat
    13141 cgccactggt gtgaaaggtg ttaaccttcg tgaaggagac acagttgttg gtgccagctt
    13201 gattactgat caagatgagg ttcttgttat cacagaaaaa ggatatggta agcgtacagt
    13261 cgctactgaa tacccaacaa aaggtcgtgg tggtaaggga atgcagacag ctaaaattac
    13321 cgaaaaaaat ggcttgctgg ccggtcttat gactgttcaa ggggatgagg atttgatgat
    13381 tatcactgat acaggtgtca tgattcgaac caatcttgcc aatatttcac aaacaggacg
    13441 tgcaactatg ggagttaaag taatgcgcct ggatcaagat gctcagatag tgactttcac
    13501 aacggttgcg gtggcagaaa aagaagaagt tgggacagaa aacgaaacag aaggtgaagc
    13561 ataatgtctc aaaaaaataa taaaaagaaa aacaagcgaa aaaatttgct gacaaatatc
    13621 ctagcaggat ttctgatatt actgtcactg gctttgattt ttaatactca aattcgaaat
    13681 attttcatag tctggaatac caataagtat caagttagcc aggtatcaaa agaaaaatta
    13741 gaagaaaatc aggatacaga aggcaatttt gactttgatt ctgtcaaagc tatctcttcg
    13801 gaagctgttc taacttctca atgggatgct caaaaattac cagttattgg gggaattgca
    13861 attcctgaat tggaaatgaa tttgccgatt tttaaaggac ttgataatgt taatctcttc
    13921 tacggagctg gtacaatgaa acgcgagcaa gtaatgggag aaggaaatta tagtctagct
    13981 agtcaccata tctttggtgt tgataatgct aataaaatgt tattttctcc tttagataat
    14041 gctaaaaatg gcatgaagat ttatctaacc gataaaaata aagtttatac ttatgaaata
    14101 cgtgaagtca aacgtgtgac accggatcgt gttgatgaag ttgatgatag agatggggtc
    14161 aatgaaatca cattagtaac ctgtgaagac cttgctgcta cagaacgtat tattgtcaaa
    14221 ggtgatttga aagaaacaaa agattattca caaacatcta atgaaatcct aacagctttc
    14281 aatcaaccat ataaacaatt ttattaatac aaatcagtga aatccatgat ttcactgatt
    14341 ttttaagatt ttcataaaaa tatagtaaaa tgaaataaga ataggacgaa tcgttcagga
    14401 cagtcaaatc gatttctaac aatgttttag aagtagaggt gtactattct agtttcaatc
    14461 tactataaac ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa
    14521 ttatcaagaa aaaagattta ggagtttatt atgatttctt cagaatttat ctcaaagatt
    14581 gaatttgctt gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt
    14641 cgttcgatgt tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct
    14701 gacttgatta ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct
    14761 tggggcttgg cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc
    14821 ttgactgctg gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta
    14881 tactgtacct tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg
    14941 gcagcctatg cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta
    15001 ggccgctcaa atgaattggt cttgcttgag gcgattttgg caaatatttt cgtaaatatt
    15061 gcgattctgt catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca
    15121 gctatttaca tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc
    15181 gcgattgtga aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg
    15241 cttcgccact ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt
    15301 cttccatatg ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac
    15361 gattgagtcg tgcttttttc attttcaaaa taaggtaata gctatttctt atatcaaaat
    15421 atagaaaact gatatttgta aactataact caaggtgcta caatatcctt aataaaatga
    15481 tatggaggtc accttatgac ttgtgatttt aaatctgaaa ctctacaact acatgctggt
    15541 caagttgtgg ctccagctac taagtctcgt gcagtgccga tttatcaaac aacatttttt
    15601 gtttttgatg acacgtagga aggtgccgat ctgtttgcct tgaggaaacc agggaacatt
    15661 tatactcgta tcaccaatcc tacaacagct gcccttgaag gtggtgttga agcgctagca
    15721 acagcatcag gtatgactgc agtgacttat acgattttgg cgcttgccca tgctggtgac
    15781 catgtagtgg ctgcttcgac tatttacggt ggaaccttca atcttttgaa agaacccctt
    15841 cctcgttatg gtatcacaac aacctttgtc gatattgata atttggagga agtagaagca
    15901 gctatcaaag acaataccaa gcttgtcttg attgaaacct tgggtaaccc cttgattaat
    15961 attccagacc tggaaaaact ggcagagatt gctcataaac atcaaatccc acttgtgtca
    16021 gacaatactt ttgcaacacc ttatttgatt aacgtcttct ctcatggcgt tgacattgcc
    16081 attcactctg cgactaagtt tatcggtggg catggtacaa ctattggaga aataattgtc
    16141 gatagtggtc gttttgactg gacggcttca gggaaattcc ctcaatttgt tgacgagggt
    16201 ccaagctgcc acaatttgag ttatactcgt gatgtgggtg cagcagcctt tattatagct
    16261 gttcgagttc aattgcttcg tgatacaggt gcagccttgt caccattcaa tgctttcctc
    16321 ttgctacaaa gacttgaaac cctttcactt cgtgtggaac gccatgtaca aaatgctgag
    16381 acaattgttg attttcttgt caaccatcct aaggtagaaa aggtaaatta tccaaaactt
    16441 gcagatagtc cttattatgc cttggctgag aaatacttgc caaaaggtgt cggttcaatc
    16501 tttaccttcc acgtcaaagg tgacgaggaa gaagcacgca aggtcattga taatttagaa
    16561 atcttttctg accttgcaaa cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca
    16621 atcactcacg gtcaattgtc agaaaaagac ctagaagcag caggtgtcac accaaatcaa
    16681 atccacttgt ctatcggtct tgaaaatgta gaagatttga ttgaagactt acgcttggcc
    16741 ttggaaaaaa tttaaagtaa aagaagataa acagtgggtt ttgactcact gtttttgatt
    16801 ttccctcagg catgatataa tggttacaga agtctagaaa gaggaatgta atgaacgaaa
    16861 tcaaatgtcc caactgtggg gaagtcttta cagtaaatga aagccagtat gctgaacttt
    16921 tgtcccaagt gagaacggca gagtttgata aggaactaca cgataggatg aggcaggaac
    16981 tggccttggc tgagcaaaag gctatgaatg agcaacagac taaactggct cagaaggatc
    17041 aagaaattgc gcaattgcag agtcaaatcc aaaactttga tacagaaaaa gaattggcca
    17101 agaaagaagt tgaacagaca agccatcagg ccttattggc taaggacaag gaagtacagg
    17161 ccttggaaaa ccagttggcg accttgcgtt tggagcatga aaatcaattg caaaagaccc
    17221 tttctgacct agaaaaagaa cgcaatcagg tcaaaaatca gctcctactg caagaaaagg
    17281 aaaatgagtt gtctttggct tctgttaagc aaaactacga agcccagctc aaagcagcca
    17341 gtgaacaagt cgagttttat aagaatttta aggctcaaca atctacaaaa gcgattgggg
    17401 aaagcctaga acagtatgca gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa
    17461 atgcttactt tgagaaggat aacaaggtct cttcgcgtgg gtctaaaggg gactttatct
    17521 tccgtgagtg tgatgaaaat ggagttgaaa tcatttctat catgtttgag atgaaaaacg
    17581 aagcggacgg aacagagaag aagcacaaga atgcagattt ttacaaggaa ttggacaagg
    17641 accgtcagga gaagaactgt gagtatgccg ttttggtgac catgcttgag gccgataatg
    17701 actactttaa cacagggatt gttgacgtca gccacgagta tgaaaaaatg tatgttgttc
    17761 gtcctcaatt ctttatccag ttgattggtc tcttacgtaa tgcggcgcta aattccctaa
    17821 aatacaagca ggagttggcc ttggttcgcg agcaaaatat tgacattacg cattttgagg
    17881 aagatttgga tgcctttaag ctagcttttg ctaagaacta taattcagct tcgactaact
    17941 ttggaaaagc tattgatgaa atcgacaagg ccatcaaacg catggaagag gttaagaaat
    18001 tcctgactac atctgaaaac caactccgtc tagctaacaa caaattggaa gatgtctctg
    18061 ttaaaaaatt gacccggaaa aatccaacaa tgaaagcgaa gttcgaagca ctgaaggggg
    18121 agtagaaagc aaaaatgaac ggtattatca acttaaaaaa agaagcagga atgacctcgc
    18181 atgatgcggt ttttaaactg cgtaagattt tgggaaccaa gaaaattggt catggtggaa
    18241 ccttggatcc ggatgtggtg ggtgttttgc cgattgcggt tggcaaggcg acacgcatgg
    18301 tcgagtttat gcaggacgag ggtaagatct atgaggggga aattactctg ggttattcca
    18361 cgacgactga ggatgctagt ggggaagtga tcgcagaaac ccctgttttg tctcccttgt
    18421 atgaaaagct tgttgatgaa gcgattgcta gcttgactgg gcctattacc cagattcccc
    18481 ctatgtattc ggcagttaag gttaatggtc gcaagctcta tgagtatgcg cgtgctggtc
    18541 aggaagtgga gcgtccagaa cgtcaggtga tcatttatca atttgagcga acaagtccga
    18601 tttcttatga tggccaactt gcccgattca cttttcgtgt aaaatgcagt aaagggactt
    18661 acatccgtac tttgtcagtt gatttgggtg aaaagcttgg ttatgcggct catatgtccc
    18721 atttgactcg tactagtgct gctggcttac aattagaaga cgctctttcc ttggaggaaa
    18781 ttgctgaaaa agtagaggct gggcaattag attttctcca tcctttagag attgggacag
    18841 gtgaccttgt caaagttttc ctaactccag aagaggctac agaagttcgc tttggtcgtt
    18901 ttattgagct agaccaaacg gacaaagaac tggctgcctt tgaagatgat aaattgttag
    18961 ccattctaga aaaacggggc aatctctata agccaaggaa ggtttttagc tagatcgttt
    19021 aggaataaaa atcgggtgat agataacaat tgcttgataa aaccccatac taatagtaga
    19081 atggttttgg gaattataat attccaattg ttgcgagttg taggtactca aataatctat
    19141 atagaaattt agaggtgtga aatgaagcaa tttaaaattc tttcagataa atatttagag
    19201 tccattacag gttctggtgg gaacttaggc ccaggatttg gtgtgataat tccatgatgc
    19261 gaaatgagtt tcgagaaagg gtggagcaac ttcttcaaca aaaagaaata aatgaaaata
    19321 gtgagttgag tcacctgttt cgtcttgcta tacaaaattt agacagaaat gaaaaatacc
    19381 aatcggtcat ggccaatttg agtcaagggt tgtcacttta cctcatgacg catcattacc
    19441 aggcacctaa gtctgtcatt gattttggtt tatggattgc caaagctcct agccaggaaa
    19501 gagggagact ggctttcttg cagatgcttg ctcaaaccct acagggcttt cgttaaaact
    19561 gtcgaacatc agagggaaga ctctggtgtt ttttgctgat gagactataa attttgtaaa
    19621 aaatgtgata gaatagacga cggataaaat aacggaggat agcatgcaaa atagaccaat
    19681 cattattgga gtgacaggtg gttctggtgg tggtaaaacc agtgtttcaa gagccatttt
    19741 atcgcatttt cctgatgaaa agatttccat gattgagcat gattcatact acaaggatca
    19801 gtctcatttg acctttgaag agcgtgtcaa aaccaactat gaccatcctt ttgcctttga
    19861 tacagacttg atgatcgagc agattaagga attgttggca gggcgtccgg tggacatccc
    19921 gacctacgac tatacagagc atacacggag tagcaagacc tatcgtcagg agcctcaaga
    19981 tgtctttatc gttgagggca ttttggtctt ggaggacaag cgtctgcgcg atttgatgga
    20041 tatcaagatt tttgtggata cggatgacga tgtgcgcatt attcgtcgta tcaagcgtga
    20101 tatggaggag cgtggccgta gccttgatag cgttattaac cagtacttag gtgtggtcaa
    20161 accaatgtac caccagttta tcgagtcaac taagcgttat gctgatatcg tcattcctga
    20221 aggggttagc aataccgtgg ctatcgacct gttgacgacc aagattgcaa agattttgga
    20281 agaagctcga aacagcaaat aatcacatgt ggaggccttg cctccttttt ctatttttcc
    20341 ctcataatgg tacataaatg gagattttta ggcatatttt tggtataata atacccatga
    20401 aagagcaagt aaatgaatag taggtggaga tggaaaagta tttatcggta acaactttga
    20461 ccaagtatct gaaaatgaaa ttcgataaag acccatactt ggaacgggtc tatttaactg
    20521 gtcaagtttc caactttcgt aaacgaccta ctcaccaata tttctcccta aaggatgacc
    20581 atgcagttat tcaagcgacc atctggtctg ggatttatca gaaattaggg tttgacctgg
    20641 aagaaggaat gaagatcaat gtgattgggc gtgtacaggt ctatgaacca agtggtagct
    20701 actccatcat cattgaaaaa gctgagcctg atggggttgg ggcgcttgcg attcagtttg
    20761 aacaacttaa gaaaaaattg acagaagaag acctgtttca agaacgcttc aagcaagctc
    20821 tgccccaatt ttctaagaga attggtgtag taaccagccg tagtggagcc gttattcgag
    20881 atattatcac gaccgtcagc aggcgattcc caggtgttga catccttctt tatccgacca
    20941 aggttcaagg tgaaggggct gcggaggaaa ttgctagaaa tattgcgcgt gctaatcaac
    21001 gggacgattt ggatttgctc attattggtc gtggaggtgg ttctattgag gatctctggg
    21061 cctttaacga agaaattgtg gtacgagcta tttttgaatc tcgtttacca gttatctcta
    21121 gtgtggggca tgagacggat gtgaccttgg cagattttgt ggcagatcga cgcgctgcaa
    21181 cgccaacagc ggcggctgaa ctggcaacac ctgtgaccaa gttggatgta ttagctcatt
    21241 tgcaaaatca ggaaaaacgg atggcaacgg cagtccgaaa tgttctatct aagaaacaag
    21301 aggctttgaa aaaatgcagt cagtctgtta tctttagaca acctgagcgc ttgtatgacg
    21361 gttatttgca acgcttggac caactgcaac tgcgtttgaa acaaagtttg cgaactcgga
    21421 tttctgataa caaacaatta gttcaagcaa gaactcatca attagtacaa ttatcacctg
    21481 ttaccaaaat ccaacgctat caagaccgtt taggacagtt ggacaagctc ttaggtagcc
    21541 aaatggcgtt agtttatgac gccaaggttg ctgaggccaa gcgactttcg gaagctttgc
    21601 tcatgttgga tactagccga atcgtggcgc gtggttatgc tattgtcaaa aaagaagagt
    21661 ccgttgtaga ttcggttgag agtttgaaga aaaaagacca agtaacgctt ttgatgcgag
    21721 atggtcaagt agaattagag gttaaagatg tcaaaacaaa agaaatttga ggaaaatcta
    21781 gcagaactgg aaaccattgt ccaaagtttg gaaaatggtg aaattgctct ggaagatgcg
    21841 attactgcct ttcaaaaggg catggtcttg tcaaaagagc tccaagctac gctggacaag
    21901 gctgaaaaga ccttggtcaa ggtcatgcaa gaagacggaa cagaaagtga ttttgaatga
    21961 aaaagcaaga aaaattagct cttgtcgagt cggctttgga agatttttat ggagaccagc
    22021 agtttgcctc tagtttacgg gagtctgttc tctattctat tcatgctggt ggcaagcgta
    22081 ttcggccttt tctcttgtta gaagttctgg aagccttgca ggttgccatc aaacctgctc
    22141 acgcgcaggt agctactgcc ttggagatga ttcatacagg gagcttgatt cacgatgacc
    22201 ttcctgctat ggatgatgac gattatcgaa gagggcggtt aaccaatcac aagaaattcg
    22261 gtgaagctat ggccattttg gctggagatg ccttattctt agacccatat gccttgattg
    22321 cgcaggcaga tttgccaagt cagatcaagg tggacttgat tgccaactta tcccttgctt
    22381 caggtagtct gggtatggtg gcagggcaag ttttggatat ggagggcgaa caccagcact
    22441 tgtctctgga agaacttcag actattcatg ccaataagac tgggaagtta ctagcctatc
    22501 ccttccaagc ggcagctatt atagctgaat tgtcacctga aatgcaggtg aagctgaaaa
    22561 ctgtgggtga attgattgga cttgcttttc aagtcagaga tgatgtactg gatgtgacag
    22621 ctagttttga ggaaatcggc aagacacctc aaaaggatct gcaggcagaa aaatcaacct
    22681 atcctgcctt gttgggcttg gaagagtcca ttgccttttg taaccaaacc ctggatcaag
    22741 ctaatgaaaa attagaagaa attgcccagc agattccctt tgaaacagaa tcgattgtaa
    22801 gtgtagtaga aagtttgaga atcaatggct aaggaaagag tggatgtact agcttataaa
    22861 caggggttgt ttgaaacgag agagcaggcc aagcgaggtg tgatggctgg cctagtcgta
    22921 gcagtcctta atggagaacg gtttgacaag ccaggagaga aaattccaga tgacaccgaa
    22981 ttaaaactca agggggagaa actcaagtat gtcagccgtg gtggtttgaa actggaaaag
    23041 gccttgcagg tctttgattt gtcggtggat ggcgcgacta cgattgatat cggggcctct
    23101 actggaggtt ttaccgatgt catgctacag aatagtgcca agttggtctt tgcagtcgat
    23161 gttggtacca atcagttggc ttggaaatta cgccaagacc cacgagttgt cagcatggag
    23221 cagttcaatt tccgctatgc tgaaaagact gatttcgagc aggagccgag ctttgccagt
    23281 attgatgtga gtttcatttc ccttagtctg attttgccag ccttgcaccg tgtcttggct
    23341 gatcaaggtc aggtggtagc acttgtcaaa cctcagtttg aggcaggacg tgagcagatt
    23401 gggaaaaatg gaattattcg agatgctaag gttcatcaga atgtccttga atctgtaaca
    23461 gctatggcag tagaggtagg tttttcagtc cttggcttgg acttttctcc catccaaggt
    23521 ggacatggaa atattgaatt tttagcgtat ttgaaaaaag aaaagtcagc aagcaatcag
    23581 attcttgctg agattaaaga agcagtagag agggcgcata gtcaatttaa aaatgaataa
    23641 aaaagagaga cttgaaaaaa ttagaagatt ggtgacagat tatcaaatcg gcacgcaaga
    23701 agaaattgta gaacatttga aagaagcagg tatcactgcc actcaggcga cggtatcccg
    23761 agatatcaaa gagttaggta ttgtcaaaat tcctttgaga gacaacacct atgtctacga
    23821 attaccaaaa tcaatcgtaa aaagtctgca actggctgaa gacaatatcg aatcggctga
    23881 attgatggat aagatgatca atctccaagt tattccagga aatacggctt ttgtaaaagc
    23941 tcagttaatc gagacttttg cagacaagat ttttagctgt ttgactgatg atagctcgat
    24001 tttagtcatt gccagaagtg gaagtctagc agaggaaatc tttgaacaag taaaaaattg
    24061 gtaggtctat atgttacttg aaatttcgat aaaaaacttt gccattattg aggctatttc
    24121 cctcaatttt gaaaagggga tgactgtcct gactggtgaa acgggtgcag ggaagtcgat
    24181 tatcattgat gccatgaata tgatgttggg agctcgtgcg acgacagatg ttattcgtca
    24241 tggtgcacca aaggcagaga ttgaggggct tttctcagtt gagaatagtc gtcttttaca
    24301 ggaaattttt gataagcaag gtttagaatt aggtgatgaa attatcatcc gtcgggaaat
    24361 cttgcaaaat ggtcgtagta tcagccgtgt aaatggtcag atggttaatc tgtctgtttt
    24421 gcgagctatt gggcaacatc ttgtagatat tcatggtcag catgaccatg aggagttaat
    24481 gcgtccccaa ctgcatatcc agatgttgga tgaatttggt gatgccgctt tttgggactt
    24541 gaaagaaacc tatcaaacga gttttgatgc ctatcggaaa atgcgcaagc aggttctgga
    24601 agtcaagaaa aaccaacagg aacacaaggc acgtatcgaa atgttggaat ttcaaatggc
    24661 agagattgag gcagcaaact tgcaggctgg agaagacttg gctctcaatc aagagcgaga
    24721 taaactcctc aaccacaaaa atattgcgga tacactgacc aatgcctaca gtatgttgga
    24781 caatgaagat ttttcaagtc tggccaatgt tcgttcagct atgaatgaca tggaaagtgt
    24841 cgaagagtat gatcctgaat accgtgaaat ttcaagctat ctgtctgaga cctactatgt
    24901 tttagaagac attagcaaac gtttggaagc tatcattgag gaccttgatt ttgatggcaa
    24961 tcgcctgatg caggttgaga atcgtttgga cctccttcat actattactc gtaagtatgg
    25021 tgggactgtt gatgatgttt tgctttattt tgccaagatt acggaagaat acaatctctt
    25081 gacaggcaat aatctttcgt ctgaggacat ggaagcagag cttaagaagt tggaagtcaa
    25141 tcttgtcaat ttggcaggtc aacttgcttc tgctcgtcat aatttggctc agcaactcga
    25201 agctgagatt aaacaagaac tgcaagatct ttatatggaa aaagcccagt ttcaggttcg
    25261 ttttagtaag ggaaaattca gtcgtgaggg aaataaaatg gttgagtttt atatttcaac
    25321 caaccctgga gaagacttta aacccttggt taaggttgct tctggagggg aattatctcg
    25381 tctcatgtta gccattaagt ctgccttttc acgtaaagaa ggcaagacta gcattgtctt
    25441 tgatgaggtg gatacgggag tttcaggtcg tgttgctcaa gctattgctc agaaaattca
    25501 taaaattggt cagcatggtc aggttttggc tatctcccat ttgccacaag taattgcgat
    25561 tgcagattat caattcttta ttgagaagat tagtaatgac cattcaacgg tttcgactgt
    25621 tcgtctcttg acggtcgaag agcgagtgga ggaagttgcc aagatgttgg caggtgatga
    25681 tgtgacagaa gcagccctga cgcaagcaag agaattgttg agaaacaggg agaaataaga
    25741 tgacagatta ttatgtaatt ggagatgttc acggaaaagc tgggatgctg gaagaccttc
    25801 tcaaaacctg ggatggtcag ccccagttgc tctttctagg tgatttgatt gacagaggtg
    25861 aggatagtca ccgtgttctt gaaatggtca aggacttggt ggacaatcaa ggggctatct
    25921 gtttgtccgg aaaccacgag tatatgtttt tgacttggct agatgaccca gaagaaagtt
    25981 atgaccatta tagtcgcaat ggtggagata caaccattaa ctctatccta ggtcgtccct
    26041 tggatgcacc agttgatgga gtagaagatg ccaagcgggt tgccgctgaa gcgtcagact
    26101 tggtcgaatt cattcgtcaa atgccatttg tagtagagac agacaagtat atatttgttc
    26161 acgcaggtat tgatttgact ttggacgact ggcatgaaac aacagattat aaaaaagtat
    26221 ggcttagaaa accattccac ggagctgaaa atcatactgg aaaaaccatt gtcttcggtc
    26281 atacaccggt ctatggtttg ttacagcaag aacgcggtac atctgagctt tggactacag
    26341 aagatggcaa gattggaatg gatggaggag ctgtttatgg tggtgtcctt cacgggattg
    26401 tctttacaga ccaaggaatg acagaacacc actttatcga aaatgacggc tttgtcgctg
    26461 aagattagta ctcctagcag ggtatggtct tgtcaaaata tcaaaaacaa tttataataa
    26521 atagataccc tgaaaggaag agaatcatga acttagaaga attgaaaaaa cgacagggga
    26581 agatccgtaa cttctctatt atcgcccata ttgaccacgg gaaatcaact ctagcagacc
    26641 gcattttgga aaaaacagag acggtttcaa gtcgtgaaat gcaggcccag cttttggata
    26701 gcatggatct agagcgggaa cgtggaatta ctattaagtt gaatgccatc gagttgaatt
    26761 acactgcaaa agatggggaa acttatattt tccacttgat tgacacacca gggcacgttg
    26821 actttaccta tgaagtttca cgttcgctag ctgcctgtga gggtgctatt ttggtggtcg
    26881 atgcggctca aggaattgag gctcaaactc ttgccaacgt ttatctggct ttggataatg
    26941 atttggaaat catgcctatc attaataaaa ttgacctgcc ggctgcagat ccggagcgcg
    27001 tgcgtacaga gattgaagat gtgattggtt tggatgccag tgaagcagtt ttggcttctg
    27061 ccaaggctgg tattgggatt gaagaaatcc tcgagcaaat tgtagaaaaa gtaccagcac
    27121 caacgggtga tgtgacggcg ccacttaagg ccttgatttt cgactctgtt tacgatgctt
    27181 accgtggggt tatcctccaa gtgcgtgtca tggacggagt ggtcaaacct ggtgataaga
    27241 ttcagctcat gagcaatagt aagacctttg atgtggccga agtcggtatt tttacaccaa
    27301 aagcggttgg tcgtgatttc cttgcgactg gtgatgttgg ttacattgcg gcgtctatca
    27361 agacggttca ggatactcgt gtgggtgata ccgttacctt ggcaaccaat cctgcggcag
    27421 aaccattaca tggttataag cagatgaatc ctatggtctt tgcgggtctc tacccaatcg
    27481 aatcaaacaa gtacaatgac ctgcgtgaag cgcttgaaaa attgcaactg aatgatgcta
    27541 gtcttcagtt tgaaccagaa acatctcagg cacttggatt tggtttccgt tgtggatttc
    27601 ttggacttct ccatatggat gttattcagg aacgtttaga gcgtgagttc aatattgacc
    27661 tcatcatgac agctccgtct gttatttaca aagttaattt gaccgacggt gagtctatgg
    27721 atgtgtctaa cccatctgag tttccagacc caactaagat tgcgaccatt gaagagcctt
    27781 atgtcaaggc gcaaatcatg gtaccacagg agttcgtcgg agcagtaatg gagctagctc
    27841 agcgtaagcg tggggacttt gtgactatgg actatattga tgataaccgt gtcaatgtta
    27901 tctatcaaat tcctcttgct gaaattgtct ttgacttctt tgataaactt aagtcttcga
    27961 cacgtggtta tgcaagcttt gactacgaat tgtcagaata tcgcccatct aagctggtga
    28021 aaatggatat ccttctcaat ggagataagg tggatgccct cagctttatc gttcacaagg
    28081 actttgccta cgaacgtggg aaactcatcg ttgataaact caagaaaatc atccctcgcc
    28141 aacaatttga agttccaatc caagcggcta ttggacacaa gattgtcgct cgtactgata
    28201 tcaaggccct tcgtaagaac gtgcttgcta aatgttatgg tggtgacgtt tctcgtaagc
    28261 gtaaactcct tgaaaaacaa aaagctggta agaaacgcat gaaatccatc ggatcagttg
    28321 aagttccgca agaagccttc ctcagcgtct tgagtatgga tgaagaataa gttatcagaa
    28381 atgcctttcc ttgttcacag gaaatttata taaaaatcaa tagtaaagca ggcttagatg
    28441 gtctgctttt ttagttttta tactcttcga aaatctcttc aaaccacgtc agcgtcgcct
    28501 taccgtatgt atggttactg acttcgtcag tttcatctac aacctcaaaa tcatgttttg
    28561 agctgacttc gtcagtttca tctacaacct cagaaccatg ttttgagctg acttcgtcag
    28621 tttcatctac aacctcaaaa tcatgttttg agctgacttc gtcagtttca tctacaacct
    28681 caaaatcatg ttttgagctg acttcgtcag tcttatctac aacctcaaaa ctgtgttttg
    28741 agtaacctgc ggctagcttc ctagtttgct ctttgatttt cattgagtat tagaacagaa
    28801 aaatgcttgg agtatttgtt tgtgtgttta tttttatata acaaactata aacaaaacaa
    28861 aaatataaaa aaagagacaa aaaagaatag aaagtaatta acaacgattt catatagtgt
    28921 tatacttat
//
LOCUS       DBEROU010000026        28649 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.026, whole genome shotgun sequence.
ACCESSION   DBEROU010000026 DBEROU010000000
VERSION     DBEROU010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 28649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28649
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.026"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..606
                     /locus_tag="ACL8CL_001236"
     CDS             <1..606
                     /locus_tag="ACL8CL_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HGS1330486.1"
                     /translation="KLRYLNQTEAASKRVMYSVSRFIEKRLGLKVNMTKTKITRPREL
                     KYLGFGFWKSSDGWKSRPHQDSVRRFKFKLKKLTQRKWSIDLTRRIEQLNLSIRGWIN
                     YFSLGNMKSIVASIDERLRTRLRVIIWKQWKKKSRRLWGLLKLGVPKWIADKVSGWGD
                     HYQLVAQKSVLKRAISKPVLEKRGLVSCLDYYLERHALKVS"
     gene            1062..2240
                     /locus_tag="ACL8CL_001237"
     CDS             1062..2240
                     /locus_tag="ACL8CL_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ryptide export MFS transporter"
                     /protein_id="HGS1330487.1"
                     /translation="MSNSFVKLLVSQLFANLADIFFRVTIIANIYIISKSVIATSLVP
                     ILIGISSFVASLLVPLVTKRLALNRVLSLSQFGKTILLAILVGMFTVMQSVAPLVTYL
                     FVVAISILDGFAAPVSYAIVPRYATDLGKANSALSMTGEAVQLIGWGLGGLLFATIGL
                     LPTTFIILVLYIISSFLMLFLPNAEVEVLESETNLEILLKGWKLVARNPRLRLFVSAN
                     LLEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISGLIAFRLSEKFLAA
                     KWESILFPLVAMAIVTLTILYFPNAQIFLLFSALVGMLSQLKEVPESVFLQETVEENH
                     LVNVYSVLEVISTLAFSVFVLLMSYIAESFGISISFWLSAICLMIEAILIYIRRDYFK
                     "
     gene            2554..2685
                     /locus_tag="ACL8CL_001238"
     CDS             2554..2685
                     /locus_tag="ACL8CL_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HGS1330488.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            2706..4256
                     /gene="dltA"
                     /locus_tag="ACL8CL_001239"
     CDS             2706..4256
                     /gene="dltA"
                     /locus_tag="ACL8CL_001239"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047473 - D-alanine [D-alanyl carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HGS1330489.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQEHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFEGLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            4253..5497
                     /gene="dltB"
                     /locus_tag="ACL8CL_001240"
     CDS             4253..5497
                     /gene="dltB"
                     /locus_tag="ACL8CL_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070395 - lipoteichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HGS1330490.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQLLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            5511..5750
                     /gene="dltC"
                     /locus_tag="ACL8CL_001241"
     CDS             5511..5750
                     /gene="dltC"
                     /locus_tag="ACL8CL_001241"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036370 - D-alanyl carrier activity
                     [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HGS1330491.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            5743..7011
                     /gene="dltD"
                     /locus_tag="ACL8CL_001242"
     CDS             5743..7011
                     /gene="dltD"
                     /locus_tag="ACL8CL_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070394 - lipoteichoic acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HGS1330492.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            7166..7606
                     /gene="adcR"
                     /locus_tag="ACL8CL_001243"
     CDS             7166..7606
                     /gene="adcR"
                     /locus_tag="ACL8CL_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HGS1330493.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            7606..8310
                     /locus_tag="ACL8CL_001244"
     CDS             7606..8310
                     /locus_tag="ACL8CL_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HGS1330494.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYSVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            8303..9109
                     /locus_tag="ACL8CL_001245"
     CDS             8303..9109
                     /locus_tag="ACL8CL_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HGS1330495.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            9119..10624
                     /gene="adcA"
                     /locus_tag="ACL8CL_001246"
     CDS             9119..10624
                     /gene="adcA"
                     /locus_tag="ACL8CL_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HGS1330496.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNVAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(11784..12557)
                     /locus_tag="ACL8CL_001247"
     CDS             complement(11784..12557)
                     /locus_tag="ACL8CL_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1330497.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPNQKLLDAVELFDGEWVSDIQ"
     gene            12733..14136
                     /locus_tag="ACL8CL_001248"
     CDS             12733..14136
                     /locus_tag="ACL8CL_001248"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase family protein"
                     /protein_id="HGS1330498.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDVDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLIDNEGKLLSQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPILTTESFSYGF
                     TNEVGKDGEITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGSTEGTAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            14251..14889
                     /locus_tag="ACL8CL_001249"
     CDS             14251..14889
                     /locus_tag="ACL8CL_001249"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HGS1330499.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            14901..15341
                     /locus_tag="ACL8CL_001250"
     CDS             14901..15341
                     /locus_tag="ACL8CL_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HGS1330500.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            15334..15765
                     /locus_tag="ACL8CL_001251"
     CDS             15334..15765
                     /locus_tag="ACL8CL_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1330501.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            15756..16226
                     /locus_tag="ACL8CL_001252"
     CDS             15756..16226
                     /locus_tag="ACL8CL_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1330502.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            16251..17024
                     /locus_tag="ACL8CL_001253"
     CDS             16251..17024
                     /locus_tag="ACL8CL_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS1330503.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            17021..17821
                     /locus_tag="ACL8CL_001254"
     CDS             17021..17821
                     /locus_tag="ACL8CL_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1330504.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            17880..20174
                     /locus_tag="ACL8CL_001255"
     CDS             17880..20174
                     /locus_tag="ACL8CL_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HGS1330505.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            20184..23300
                     /locus_tag="ACL8CL_001256"
     CDS             20184..23300
                     /locus_tag="ACL8CL_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 98 C-terminal
                     domain-containing protein"
                     /protein_id="HGS1330506.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNDYEVFTQHIDSLESNNLSIDLKGLKGKKVRISLRSAGIPLSLA
                     EVEVYTYK"
     gene            23383..25149
                     /locus_tag="ACL8CL_001257"
     CDS             23383..25149
                     /locus_tag="ACL8CL_001257"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HGS1330507.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAIWGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYCACQLLGPLHK"
     gene            25295..26446
                     /locus_tag="ACL8CL_001258"
     CDS             25295..26446
                     /locus_tag="ACL8CL_001258"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HGS1330508.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKTLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            26576..27469
                     /locus_tag="ACL8CL_001259"
     CDS             26576..27469
                     /locus_tag="ACL8CL_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000963957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HGS1330509.1"
                     /translation="MAIFFMIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKV
                     ANSGIHIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTD
                     AYYKLIRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYII
                     VKTLITKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEKDRLHGVGI
                     AQQRKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPN
                     TPNGVDDIRTQILSALRAEKK"
     gene            complement(27506..27664)
                     /locus_tag="ACL8CL_001260"
     CDS             complement(27506..27664)
                     /locus_tag="ACL8CL_001260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330510.1"
                     /translation="MKIKEQTRKLATGCSKHRFEVVDKTDEVSSKHCFEVADRTDEVS
                     NIYTARRR"
     gene            27868..28119
                     /locus_tag="ACL8CL_001261"
     CDS             27868..28119
                     /locus_tag="ACL8CL_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HGS1330511.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQDTE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            28107..28397
                     /locus_tag="ACL8CL_001262"
     CDS             28107..28397
                     /locus_tag="ACL8CL_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZmpA/ZmpB/ZmpC family metallo-endopeptidase"
                     /protein_id="HGS1330512.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQAEEEYKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQELMNKAVKDEVAQI"
BASE COUNT         9035 a         4690 c         6091 g         8833 t
ORIGIN      
        1 aagttgagat acctaaacca gacggaggca gcctctaagc gtgtgatgta ttcagtcagt
       61 cgttttattg agaaacggct aggtttgaaa gtaaacatga ccaagactaa gattaccaga
      121 ccaagagagt tgaaatatct aggttttggg ttctggaaat catcagatgg ttggaaaagc
      181 cgtccacatc aagatagtgt tcggagattt aagtttaaat tgaagaaact aacacagagg
      241 aaatggagta tagacctaac aagacgtatt gagcaactga atttgtctat tcgaggatgg
      301 ataaactatt tctcattggg aaatatgaaa agtatagtcg ccagcataga tgagcgcttg
      361 cgtactcgcc tacgagtgat tatctggaag caatggaaga agaaatcgag acgattatgg
      421 ggattgctta agttaggagt tcctaaatgg atagcagata aggtatctgg ctggggcgac
      481 cattatcaat tagtagctca gaagtcggta cttaaacgtg ctatatcaaa accagtcctg
      541 gaaaaacgtg gactggtttc gtgtttggat tattaccttg aacgacatgc gttaaaagtt
      601 agttgaaccg ccgtatgccg aacggcacgt acggtggtgt gagaggggct agagattatc
      661 ccctactcga ttaactcccc tgaaatttat tttaattatg caaatttcac gtatttttga
      721 tgctgagacg acgatcctgg gaacttttca gatatttttt tgactatcta aatctatcat
      781 tagaaaagct tagagcgcca aaggatttga gcgtttttct gatttttaag actttttcca
      841 gtctcttttt cgattgaaga tgtaattatt ctactaacta actaacttct tagtactagc
      901 caacaacgat aatcataatt cctcctaaaa ttaggaataa taaaggcaat agtttttgtt
      961 ttttcatgta aaaaacctca cttttgtttt ctgctatttt atgctaaaat attaaaaatc
     1021 aaatttaatt ccaaagtttg taactaaagg gggagcgcta catgtctaat tcatttgtca
     1081 agttgttagt ctctcaatta tttgcaaatt tagcagatat tttctttaga gtaacaatca
     1141 ttgctaacat atacattatt tcaaaatcag taattgccac atcactagtt cctatcttaa
     1201 taggaatatc ctcttttgtt gcgagtcttt tagttccgtt ggttactaaa aggttagcgc
     1261 taaatagggt tttatcttta tctcaatttg gaaagactat attattggcg atactggtag
     1321 gaatgtttac cgtaatgcaa tccgtagcgc ctttggtgac ctatctattt gttgttgcaa
     1381 tttccatact agatggtttt gcagcacccg tttcctatgc tattgtgcca cgctatgcga
     1441 ccgatttggg taaggctaat tcagccttat caatgactgg tgaagctgtt caattgatag
     1501 gttggggatt aggtggactc ttgtttgcaa caattggtct gttacctacc acgtttatca
     1561 ttttagtctt gtatatcatt tctagctttc tgatgttatt tcttcctaac gctgaagtgg
     1621 aggtgttaga gtcagaaact aatcttgaaa ttttgctcaa aggttggaag ttagttgcta
     1681 gaaatcctag attaagactt tttgtatcag caaatttatt ggaaattttt tcaaatacga
     1741 tttgggtttc ttccattata cttgtttttg taacggagtt attaaataaa acggaaagtt
     1801 actggggata ttctaataca gcatactcta ttggtattat aattagtggc ttaattgctt
     1861 ttaggctatc tgaaaagttc cttgctgcta aatgggaaag tattcttttt cctcttgtag
     1921 ctatggcaat agtgacactg actattctat attttccaaa cgcacagata tttttattat
     1981 tttcagcttt agttggtatg ttatcgcaac taaaagaagt tcctgaaagt gtatttcttc
     2041 aggaaacagt agaggaaaat catttagtta atgtctattc cgttcttgaa gtgatttcta
     2101 cattagcatt ttcagtattt gttttgctaa tgagctatat tgctgagagt tttggtatta
     2161 gcatcagttt ttggctatca gccatttgtc tgatgataga agctatttta atttatatca
     2221 gacgagatta ttttaaatga gagagtggtt aaaaagtctt taaaaatcag aaaagcgcat
     2281 agtatcaggt gttgaaaaaa cttgatattg tgtgttttat tatgggaaga tttacttcat
     2341 tgtctcctga aatggagttt tgaagcagga tcactctatt tgaaaatata catagaaaaa
     2401 ctgtataatc cttaaaataa gctgaaatct attggaaaca tacggtaaac aattgaaaag
     2461 atactatata aagtggtaca atggtagaaa aataagctag taagaatcac tcttatttta
     2521 gcgaaaaatt acagaaatga atggtttttc ttgatgaaac agagaaaaga attgtacctc
     2581 tttcttggtc ggacagcctt gtattttctt atctttctag ggctgcttta cttctttagc
     2641 tatcttggtc agggtcaagg aagctttatc tataatgaat tttaacttga aggagaatcc
     2701 ctattgtgtc aaataaacca atagcagata tgattgaaac cattgagcat tttgctcaga
     2761 cacagcctag ctatcctgtc tataatgttt tggggcagga acacacttat ggcgatttaa
     2821 aggctgattc ggatagtttg gctgcagtca ttgaccaact aggcttgcct gagaagtctc
     2881 ctgtggttgt ttttggtggc caagaatatg aaatgttggc aacctttgta gcgctgacta
     2941 agtcaggtca tgcctacatt ccaattgata gccattcggc cttggagcga gtttcagcta
     3001 ttttagaagt agcagagcca agcttgatta ttgccatttc agcctttccc ttggagcagg
     3061 tttctacacc aatgataaat ctagctcagg ttcaagaagc ctttgcccaa gggaataact
     3121 atgaaatcac gcatccagtc aagggagatg ataattacta cattatcttt acttctggta
     3181 cgactggtaa gcctaaggga gtgcagattt cacatgataa tctcctcagc tttacaaact
     3241 ggatgattac ggataaggaa tttgcgacac cgagtcgtcc gcaaatgctg gcacagccac
     3301 cttattcttt tgacttgtct gtcatgtatt gggcaccgac cttggcactt ggtggtacgc
     3361 ttttcactct tccttcagtc atcactcagg actttaagca actctttgcg gctatctttt
     3421 cattgccaat cgctatctgg acatcaacac catcctttgc agatatggcc atgttgtctg
     3481 aatacttcaa cagtgagaaa atgcctggaa tcacgcattt ctactttgat ggtgaagaat
     3541 tgacggtcaa aacagctcaa aaactgcgcg agcgtttccc aaatgcccgt atcatcaatg
     3601 cttacggccc aacagaagcg acagtagctc tgtcagcagt tgccgtgaca gacgagatgt
     3661 tagcgactct caaacgccta ccaatcggct ataccaaggc tgattctcca acctttatca
     3721 ttgacgagga aggaaataaa ctgccaaatg gcgaacaggg agaaatcatt gtttctgggc
     3781 cagctgtttc aaaaggttat atgaacaatc ctgaaaaaac agcagaagcc ttctttgagt
     3841 ttgaaggtct gccagcctat cacacaggcg atgtgggaac catgacagat gagggcttgc
     3901 ttctctacgg cggacgcatg gacttccaga ttaagtttaa cggttaccgc attgagttag
     3961 aagatgtctc tcaaaacctc aacaagtctc gctttatcga atctgctgtc gcagtaccgc
     4021 gctataacaa ggaccacaag gtacaaaatc tattggctta tgtcatctta aaagacggtg
     4081 ttcgtgagca gtttgagcga gatatcgata ttaccaaggc catcaaggaa gacctgacag
     4141 acatcatgat gtcctacatg atgccatcta aattccttta ccgagacagt ttgccactga
     4201 ctccaaatgg aaagattgac atcaaaggat tgattaacga ggtgaataag agatgatgga
     4261 gttttttcaa cagcttcctc atttagagcc atatggcaat cctcagtatt ttgtttatgt
     4321 gattgctgca accttgccca tctttatagg cctctttttc aagaaacgct ttgcctggta
     4381 tgaagtgttg gtaagtctct tctttattgt caccatgttg gtgggtggaa agaccaatca
     4441 actagctgcc ttgggtattt acctttgctg ggaaatattg ctcctgcttt tctacaagca
     4501 ttatcgaaaa agcaaggatg gcaagtgggt cttctactta gttagttttc tgtccctact
     4561 tccgattatc tttgtcaagg tgcaaccagc tatcaatgga acgcagtctt tgcttgggtt
     4621 cttgggaatt tcttacctga cctttcgttc ggttgggatt atcatcgagc tgagagatgg
     4681 agtgattaag gattttaccc tctgggaatt cctccgtttc cttctcttca tgccaacttt
     4741 ctcgagtggt ccaatcgatc gttttaagcg atttaatgaa aattatcagg ccattcctga
     4801 gcgagatgag ttgatggata tgctggatga atctgtccgc tatatcatgc ggggcttttt
     4861 gtataagttt atcctagctc atgttttagg agagacctta ctacctcctc tgaagaattt
     4921 agccttgcag tcaggtggct tctttaatct ctatgccttg gcagttatgt atacttttgg
     4981 tctggagctc ttctttgact ttgcaggtta ttctatgttt gctttggcca tctcaaactt
     5041 gatgggaatc cgtagcccta tcaactttaa caagcccttt ttatcaaggg atttaaagga
     5101 gttttggaat cgctggcata tgagtctgtc cttctggttc cgtgactttg tctttatgcg
     5161 aatggtgatg gtgttaacca gaaagaaagt ctttaaaaat cgtaatgtaa cctcaagcat
     5221 ggcctacatt gtaaatatgc tgattatggg attttggcat ggtgtgacct ggtactatat
     5281 cgcctatgga ctctttcatg gactaggctt ggtcatcaat gatgcctggg ttcgcaagaa
     5341 aaaaacgctc aataaggaac ggaaaaaagc agggaaggct gccctacctg agaatcgctg
     5401 gattcagttg cttggcatgg ttgtcacttt ccatgttgtc atgttgtcat tcttaatctt
     5461 ttctggattc ttgaataatc tatggtttaa aaaataaaag gaaataaaat atggatatca
     5521 aatcagaagt tattgaaatt attgatgagt tgtttatgga agatgtttct gacatgatgg
     5581 atgaagatct ttttgatgca ggtgtcttgg atagtatggg aacggttgag ttgattgtgg
     5641 agattgagaa ccgttttgac attcgtgtcc ctgtaacaga gtttggtcgt gacgactgga
     5701 atacagctaa taaaatcata gctggtattg tggagctaca aaatgcttaa acgcttatgg
     5761 atgatcttcg gaccggtctt gatcgctggt ttgttggttt ttctgctcat tttcttttat
     5821 cctactgaga tgcatcataa tctaggagct gaaaagcgtt cagcagtggc tactactatc
     5881 gatagtttta aggagcgaag tcaaaaagtc agagcactat ctgatccaaa tgtgcgtttt
     5941 gttcccttct ttggctctag tgaatggctt cgttttgacg gtgctcatcc tgcggtatta
     6001 gctgagaaat acaatcgttc ctaccgtcct tatcttttag gacagggggg agctgcatcg
     6061 cttaaccaat attttggaat gcaacagatg ttaccacagc tggagaataa acaagttgtg
     6121 tatgttatct cacctcagtg gttcagtaaa aatggctatg atccagcagc cttccagcag
     6181 tattttaatg gagaccagtt gactagtttt ctgaaacatc aatctgggga tcaggctagt
     6241 caatatgcag cgactcgctt actgcaacag ttcccaaacg tagctatgaa ggacctggtt
     6301 cagaagttgg caagtaaaga agaattgtcg acagcagaca atgaaatgat tgaattattg
     6361 gctcgtttta atgaacgcca agcttccttt tttggtcagt tttcggttag aggctatgtt
     6421 aactacgata agcatgtagc taagtattta aaaatcttgc cagaccagtt ttcttatcag
     6481 gcaatagaag atgttgtcaa agcagatgct gaaaaaaata cttccaataa tgagatggga
     6541 atggaaaatt atttctataa tgagcagatc aagaaggatt tgaagaaatt aaaggattct
     6601 cagaaaagct ttacctatct caagtcgcca gagtataatg acttgcagtt ggttttaaca
     6661 cagttttcta aatctaaggt aaacccgatt tttatcattc cacctgttaa taaaaaatgg
     6721 atggactatg ctggtctacg agaggatatg taccaacaaa cggtgcagaa gattcgctac
     6781 cagttagaaa gtcaaggttt taccaatata gcagattttt ctaaggacgg cggggagcct
     6841 ttctttatga aggacaccat tcaccttggt tggttgggtt ggttggcttt tgacaaggca
     6901 gttgatcctt tcctatccaa tcccacacca gctccgactt accatctgaa tgagcgcttt
     6961 ttcagcaaag attgggcgac ttatgatgga gatgtcaaag aatttcaata gataatataa
     7021 tatagaagag ttcaagaatg aaactagttt tcacgttttt tcttgactct ttttttgctt
     7081 gtgatataat taactggtaa acaaaatgtc aaagaatagt atttttctct taaaaaagag
     7141 aagtctaaaa ggaaaggagt cagatatgag acagctagca aaggatatca atgctttttt
     7201 gaatgaggtg attttgcagg cggaaaatca gcatgaaatc ctaataggtc attgcactag
     7261 cgaggtggcc ctgaccaata ctcaggagca tatccttatg ctcttgtcag aggaatcttt
     7321 aacaaattca gaattggccc gtcgtctcaa tgtcagtcag gcggcagtta ccaaggccat
     7381 taagtctttg gtcaaggaag ggatgttgga aacatctaaa gattctaaag atgcgcgtgt
     7441 gattttttat cagttgactg acttggctcg tccaatcgct gaggagcacc accatcacca
     7501 tgagcataca cttttaacct atgaacaagt ggcgactcag tttactccaa atgaacaaaa
     7561 agtgattcag cggtttttga ctgctttagt aggagaaatc aaataatgag atatattacg
     7621 gtagaggatt tgtccttcta ttatgataag gagcctgttc ttgaacatat caattatagt
     7681 gttgatagtg gggaatttgt taccttgact ggggaaaatg gagcggctaa gacgacgctc
     7741 atcaaggcta gtcttggaat tctgcaacca cgcattggaa aggtggctat ttcaaagaca
     7801 aatacgcaag gtaagaaatt gagaatagcc tatcttcctc aacaaattgc cagttttaat
     7861 gctggttttc caagtacggt ctatgaattt gtcaagtcgg gtcgctatcc gagaaaaggc
     7921 tggttccgtc gtttgaatgc tcatgatgag gagcatatca aggctagtct ggactcagtt
     7981 ggcatgtggg aacatcgaga caaacgcttg gggtctctat ctgggggaca aaagcagcga
     8041 gcggtgattg cgcgtatgtt tgcttctgac cctgatgtgt ttatcctaga cgagccgaca
     8101 acggggatgg atgcaggaag taaaaatgaa ttttacgaac tcatgcacca cagcgcccat
     8161 catcatggca aggctgtttt gatgattacc catgaccctg aagaagttaa ggattatgcg
     8221 gatcgcaata ttcatctagt ccgtaaccaa gactcgccat ggcgttgttt caatgttcat
     8281 gagaatggcc aggaggtggg ccatgcttag tttattatct tacgacttta tacaacgcgc
     8341 ctttttggcg gttattgcta tgagtctttt ctcaccggta ttgggaacct tcctcatctt
     8401 gcgtcgtcag agtttgatga gtgataccct tagccacgtc tcactttcag gtgtagcctt
     8461 tggtctggtt ttggggattt ctccaactgt ttctactatt gccattgtct tgattgcggc
     8521 ggtctttctg gagtatctcc gtacggttta caagagcttt atggaaatcg ggacagctat
     8581 cctcatgtca acaggtctgg ctgtttctct gattgtcatg agcaagggta aaagctcgag
     8641 ttcaatgagt ttggaccaat atctctttgg ttcgatcgtg actatcagtg aagaacaggt
     8701 catttccctc tttgtcattg cggcggttgt tttgattttg acctttctct ttcttcgtcc
     8761 tatgtatatc ttaacttttg acgaagatac ggcctttgtg gatggcttgc cagttcgtac
     8821 catgtccatt ctttttaaca tggtgacagg ggtggctatt gcccttatga ttcctgcagc
     8881 aggagctctt ctggtatcga ccattatggt cttgccagct agtattgccc tgcgtctggg
     8941 gaaaaacttt aaatcggtta tgctgcttgc cagtgcgatt ggctttttgg gaatggtagc
     9001 aggactttac atttcctact atgcagaaac acctgcaagt gcaagtatta ccattatttt
     9061 tgtaactgtc tttatactaa tcagtttagt aagacgtttt atcaaatagg agacgaacgt
     9121 gaaaaaaatt agcttattac tagccagtct atgtgccttg tttttagtgg cttgttccaa
     9181 tcaaaaacag gcagatggta aactcaatat cgtgacaacc ttttaccctg tctatgaatt
     9241 taccaagcaa gttgcaggag atacggctaa tgtagaactc ctaatcggtg ctgggacaga
     9301 acctcatgaa tacgaaccat ctgccaaggc agttgccaaa atccaagatg cagatacctt
     9361 cgtttatgaa aatgaaaaca tggaaacatg ggtacctaaa ttgctagata ccttggataa
     9421 gaaaaaagtg aaaaccatca aggcgacagg cgatatgttg ctcttgccag gtggcgagga
     9481 agaagaggga gaccatgacc atggagaaga aggtcatcac catgagtttg acccccatgt
     9541 ttggttatca ccagttcgtg ccattaaact agtagagcac attcgtgaca gcttgtcagc
     9601 agattatcct gataaaaaag agacctttga gaagaatgca gctgcctata tcgaaaaatt
     9661 gcaagccttg gataaggctt acgcagaagg cttgtctcaa gcaaaacaaa agagctttgt
     9721 gactcaacac gcagccttta actatcttgc cttggactat ggactcaaac aagtcgcaat
     9781 ctcaggactt tctccagatg cagagccatc agctgctcgc ttggcagaat tgacagagta
     9841 tgtcaagaaa aataaaatcg cctatatcta ttttgaagaa aatgcctcac aagcccttgc
     9901 taacacactt tcaaaagaag caggtgtcaa aactgatgtc ctcaatcctt tagaaagtct
     9961 gacagaagag gacaccaagg ctggagaaaa ctacatttcc gtgatggaga aaaacctcaa
    10021 ggctttgaaa caaacaacag accaagaagg cccagcaatc gaacctgaaa aggcagagga
    10081 taccaagaca gtccaaaatg gttacttcga ggatgcagct gtcaaggacc gcaccttgag
    10141 tgactatgca ggtaactggc aatcagttta tcctttcctt gaagacggca cgtttgacca
    10201 agtctttgac tacaaggcta agttgactgg taagatgacc caggctgagt acaaggctta
    10261 ctatacaaaa ggctatcaga cagatgtgac taagattaac attactgata atactatgga
    10321 atttgttcaa ggtggacaaa gcaagaaata cacttacaag tatgtcggta agaaaatttt
    10381 gacttacaag aaaggcaatc gtggcgtgcg ttttctcttt gaagccacag atgctgacgc
    10441 tggacaattc aagtatgttc agtttagtga ccacaatgtt gccccagtta aggcagaaca
    10501 tttccatatc ttctttggag gcacaagcca agaagccctc tttgaagaaa tggacaactg
    10561 gccaacctac tacccagata acctatctgg ccaagaaatc gcccaagaaa tgttggcgca
    10621 ttgatgagaa atcgactagt tcataagagc tagtcggttt tttgatacta gatggctaac
    10681 ttcattatag acctttcaga actttaaaca ataaataaaa ctcttgaaat atctgggctt
    10741 atatgctaga atgtagttag tgtataaggt tcattagaac accaaagccc ctaactagtg
    10801 ccatagttaa gggctttttt gacgtatctt agcgatttaa cgggtgttta taggctagtc
    10861 attcggtcta cttcttacca tctagccatt tgcagacgaa atacaagata attccagcta
    10921 taacatctgc tataatagta agagcaagct ctggtataag gctcattagt ttcacctcct
    10981 ctcacgaacc gtaaggaacg taatcggtaa ccgatgacaa aaatagtata tcacactaca
    11041 ttcaggtcat caaccttact taatgcttga actattgtag attatatgct agaatgtagt
    11101 taattgataa ggttcattag aacaccaaag cccctaacta tggccgtagt taagggcttt
    11161 tttgacgtat cttagcgatt taacgggtgt ttataggcta gtcagtcggt ctactcctta
    11221 ctatctagcc atctgcagac gaaatacaag ataattccag ctataacatc cgctataata
    11281 gtaagagcga gctctatgat aaggctcatt agtttcacct cctctcacga acccatagga
    11341 acgtaatcgg taaccgatga caaaaatagt ataccacaat acatttagat catcaaggtc
    11401 acttaattct tgaaatatca gatctaagag aaaaatcttt aaaatcagaa aaacgcataa
    11461 tatcaggtgt gcaaaaactt gatactatgc gttttattgt gggaaggttt actccatttt
    11521 ctcctgaaat tgagtttttg tccagcctct gtttttaggg ttgctaagaa aataatgtca
    11581 tgtggtgaat atttgtaaat cagtcagcag acagaacgat actcttcgaa aatctcttca
    11641 catcatgtca gctttgtctt tccgtatata tgtgactgac ttcatcagtt ctatctacaa
    11701 cctcaaaaca gtgtttcgag ctgacttcat cagttttcaa atctgtactg tgagcaagct
    11761 gagactagct tcctatttga tttttattga atatcagaaa cccattctcc atcaaataat
    11821 tcgactgcgt ctaataattt ttgatttggc aaggtgtcag aaataaaggt tgtgtatttg
    11881 gagaggggat taattttaaa aaatccagtc ttgtaaaatt tagaactatc aatcagtaag
    11941 atggtttcat gggctttgtc aataatattc ttttttgaaa tagcttggct gagagaagct
    12001 tcataaacat attggtcatc aatacctctt gctgaacaaa atgctaaatc gatattaaaa
    12061 tgatctaata aagaattttc cttatcatag ttgaccacgg aacaggattg atgtttgacc
    12121 tcgccagatg tgataaagat tttggagcta tctttaacag tttcagatag ggtttgtgca
    12181 gtatgtaaac catttgtaaa aataatcaaa ttatcaagtt cagaaagata gggacaaagt
    12241 tcgtagacag tagtactaga atctagatag atacacatac cagaccgaat aaagtcttta
    12301 gcgagactag cgattagtct tttttgccta gtactttctc cttcccgtat ttgatgagaa
    12361 agttcaattg tgttcataga ggacagggtc acgtatccgt gctttctttt gataagacct
    12421 tgattttcta agaaaattaa atcacgacgt aaggtacttg tgctggagaa agtgatttct
    12481 gccagctctt ttacggcaat tctttttttc tttttgatga tttcaatcaa ttcaagtaca
    12541 cgttcatctt ttatcataag ctcctcctaa tttatcattt caactatatt atagcacaaa
    12601 ttggaggaat gtgaattatt tttatgaata ttgggttaac atttgaacat tattcaagta
    12661 agcgttcaca tattgaaaaa ataaaacgtg gggattataa taaagttaat caaggacgaa
    12721 gagagaagaa aaatggaagc ggttttagca atagatttag gtgcgacttc tggaagagca
    12781 atcgttggtt acctttctga aaataaacta gtaatggaag aaataaatcg cttttctaat
    12841 ctacctatta gagtaaaagg gcatttatct tgggatgttg actttctact agctaaaatt
    12901 cttgaaagta tccgcttggc taatactagt tacaagattt tatctatcgg tattgacaca
    12961 tggggagttg attttggact gattgataat gaaggtaagc tgttatcaca acctgttcat
    13021 tatcgtgatg aaagaacaaa gggagtgtta aaggaaatat ctgaaatgac tgaattagaa
    13081 aaactgtatt cagagacagg aaatcagatt atggagataa ataccttgtt tcaactcttt
    13141 aaggcacgtc aagaatctcc tgactctttc tataagacca ataagattct tttaatgcca
    13201 gatttgttta attatctctt gacaggtaag tttgctacag aaaaaagcat tgcttcaaca
    13261 actcaattat ttgatcctag gagtcaaaac tggaatcaga atatcttaaa actatttgaa
    13321 ttggattcat ctttacttcc tgagattgtt tcagagggaa atgttcttgg aaggataaaa
    13381 aaggagtatg gtttaggcga tattcctgtt gtgaatgttt gtagtcatga tacagcaagt
    13441 gcgattgtct cagtacctaa gacagaaggt agtttattta tttcatcagg tacttggtct
    13501 ttggttggag tggaacttac ttcaccgatt cttactaccg aatccttcag ttatggattt
    13561 acaaatgaag tcggtaaaga tggagagatt acatttctga agaattgtac agggttgtgg
    13621 atcatagagg aactaagacg ttcatttgaa cgaagaggga aagcctattc ttttgatgat
    13681 attaggacaa tggtggagaa agaaaaagaa aatcttcctc tgattgatac tgaatcaaca
    13741 gaatttgcaa cagaatctga tatgcacaag actttgacag aatatctagc ttatcatcat
    13801 gaaactagag agtggacaga tggacaacta tttaagattg tttatgaaag cctagctgaa
    13861 acgtatagga aagcgataga gttattagaa gaactaactc ataaggttta taagaggata
    13921 tatgtgattg gaggaggtgc tagagccagt tactttaacc aaatgattgc tgatagaact
    13981 ggtaaagagg ttcttacagg ttcgactgag ggtacagctg tggggaatat tgttgtgcag
    14041 ctcatagcta tgggacaatt aaaagggatg gaagaagctc accatgttat tgaggagttt
    14101 ctacaattag agagttatta ctcccaaaag aattaaaaag attgagagtt tgtaaatttg
    14161 cctccctccc ccttcttaag ttttgtacaa gaagggggga taattggtga attgaaaaat
    14221 atttagtgtt ttgatatgag gaggacaaga atgtcagatg taaaacaaga attaattaag
    14281 tatggcaaga agctagtaga aacagatttg acgaaaggaa caggtgggaa tctcagtgtt
    14341 ttcgatcgtg aaaaacaatt gatggcaatt accccgtcgg gtattgattt ctttgaaatc
    14401 aaagaatccg atattgtagt gatggatatt aatggaaatg ttgtagaggg agaacgcttg
    14461 ccatctagcg aatggtatat gcatttgatt caatatcaaa ctcgtgatga tatcgatgca
    14521 attatccatg ctcatacaac ttatgcaaca gtattagctt gtctcagaga accacttccg
    14581 gctagtcatt atatgattgc agtggcaggg aaagatgttc gggtagctga gtatgcaaca
    14641 tatggtacga aagaattggc tgtgaatgca gctaaagcaa tggaaggtcg tagagcagtt
    14701 ttactagcga atcatggaat tttagcaggt gcacaaaatt tattgaatgc atttaatatt
    14761 gttgaagaag ttgaatattg tgcaaaaatt tattgtttag ctaagaattt tggagagcca
    14821 gtagttcttc ctgatgagga gatggattta atggcagaaa aatttaaaac atacggtcag
    14881 agaaaatagg gaggatatta atgttaaaac atataccgaa aaatatttct ccagatttat
    14941 tgaagacttt aatggaaatg ggacatggag atgaaatagt attagctgac gcgaattatc
    15001 cttctgcctc atgtgcaaat aagctaattc gttgtgatgg cgtaaatatt ccagaattat
    15061 tagattccat tctgtattta atgccattag atagttacgt cgatagttca attcagttta
    15121 tgaacgttgt ttcgggtgat gatattccta agatatgggg tacctataga cagatgattg
    15181 aaggtcatgg tacagatctt aaaacgatta cttatcttag aagagaagac ttttatgaac
    15241 gtagtaagaa agcttatgct attgttgcta caggagaaac ttcactttat gctaatatta
    15301 tccttaagaa aggagtagtt gttgaaagag aaaatgttta atagaggaat tttagttgcc
    15361 agtcatggta attttgctag cggagctctc atgaccgcag aaatgtttgt tggtgagaca
    15421 acaaatgata gagttaggac attaggtttg atgcctggag agaatattgt agagtttgaa
    15481 cattatttta aaaatcaagt aaatgaactg ttagactcaa atcaagaggt tatcgttttg
    15541 actgacttga ttggaggaag tcctaataat gtggctttgt cacggttttt aaatttggat
    15601 tcagttgata ttgtaacagg gtttaatatc cctctcctag tggaattaat atcaagttat
    15661 gattcaaaaa tcaatttaga agaaattgtt caaaatgctc aaaatagttt gtttaatgtt
    15721 aaacaacaac ttaacgtaga ggaggaagag gatttatgtc tatagagttt gttcgtattg
    15781 atgaccgtct ggtacatggt caagttgtca ctacgtggct aaaaaagtat gatattgagc
    15841 aagttatcat tgttaatgat cgcatctcag aagataaaac acgacaatct attttaaaga
    15901 tttctgcacc ggtaggttta aaaattgttt tctttagtgt aaaacggttt gtggaagttt
    15961 taaactctgt gccaataaaa aagagaacaa tgctgatata tacaaatcca aaagatgtgt
    16021 atgattctat tgaaggaaat ttaaaattgg agtacctcaa tgtaggacag atgagtaaaa
    16081 cggaggaaaa tgaaaaggta acgggaggtg tagctctagg tgaagaagac aaatattatt
    16141 ttaagaaaat agttgataag ggaacgagag ttgaaattca aatggttcct aatgataaag
    16201 ttacaatgtt ggaaaaattt ttataaaaat aatttaagga ggtacagtat atgctattca
    16261 cacaagcatt actggtgaca ttagttggga ttattgccac tattgactat aatggaccgt
    16321 tatttatgat tcaccgtccg ttagttacaa gtgcaatggt tggcttagtc ttaggagatt
    16381 tcacccaagg tgttcttatt ggttcagctc ttgaattaac ttggctcggt gtaacaggta
    16441 ttggaggtta cactccacca gatactattt caggtgcgat tattggtact gcatttggta
    16501 ttttatctgg tcaaggagaa actgctggta tcgctatagc agttccaatt gcagttgcta
    16561 cccaacagtt ggatgttctt gcaaaaactt tagatgttta ttttgtgaaa aaagctgata
    16621 atgatgctaa aaacggagat tattcaaaga tcggttttta tcattattca agtttggttt
    16681 taatcacgtt atttaaaatt gtaccaattt tcctagctat tatgcttgga ggggaatatg
    16741 tggcagactt gtttgctaag gttccaccaa tcgttatgca gggacttaac tctgcaggtg
    16801 ctttactacc ttcaattggt tttggtatgc ttttaaatat gatgctcaag aaaaatatgt
    16861 gggtattctt gttgattgga ttcatttgtt ctgtgtatgg aggaatgtca accattggga
    16921 tctcactagt tggtattgcg gtagcatact tctacgatat gattggaagt aaaccacaag
    16981 aaacaacttc aagtagtgat gttgaggagg atcttgatct atgatgaata ataaagtaac
    17041 taaagttgaa cttaaaaaag ttttcaaacg aagttttatg tatggttctt catggaacta
    17101 tgagagaatg cagaacctag gttttctata tacaattctt ccagtattga aaaaactata
    17161 cccagacaaa gattcagctt ctcctgcaat gaaacgtcac cttgagtttt tcaatactca
    17221 tcaaacagcg gcaccattta ttcttggagt tacttccgct atggaagaac aagaaggaaa
    17281 tgaaggtgca gcttcaatta ctggtattaa agttggcttg atggggccac tggctggtct
    17341 aggagatagt ttgttctggc tgacactagt tcctatctgt tttagtattg gtgcgtctta
    17401 ttctaaagac ggcggtgctt taggtatctt tatcgcctta atattgttta atattattaa
    17461 tattcctgtt aaatatttcg gtttgaaata tgggtatact aagggttcta gtcttatcca
    17521 agaaaataat acaaaaggaa cattgaatcg cgttacgagt atggcgacag cattagggct
    17581 agtactagtg ggtggtttga ttccatcaat ggttggtatt aattttggat tagaatttaa
    17641 gcagggggaa cttgttattt ctgttcaaga aatgattaca aaattaattc caggatttat
    17701 ccctatggct ttgactttat taatgtgtaa attaattaga aaaggaaaga atccggttgt
    17761 actaatcttt agtgttatgg ctattggagt tatcctagtt gttttaggaa ttttgaagta
    17821 gtagaaagtg tggaggtggt atttgggata tcacctccat tttggaagag aggtaaagag
    17881 tgaaattatg gtataagaaa gctgcctcaa attggaatga agccttgccg attgggaacg
    17941 gtcatttagg tggtatgatt tatggttcag ctacaaaaga atgtattcaa ctaaacgatg
    18001 agactatttg gtatagagga aagtcagata gaaataatcc agactcacta ttgcatctta
    18061 aaaaaattcg ggaatatctt ttagatggag aaattcagaa agccgaagaa ttgataaagt
    18121 taacaatgtt tgctacccca agagatcaaa gccactatga attacttggg gaactttaca
    18181 ttgagcatat agatattcag tcttgtgctc tttcattgta tgaaagagag ctagatttag
    18241 atacagctat ttctaatgtt gtgtttgagc ctaatagttg taatttacaa ataaaaagag
    18301 aatattttac gagttttaat aagaatattt tatgttgccg tatagtgtca tcagttcaaa
    18361 acacattaaa tttaaacatt aatttgggta gaaataaacg gtttaatgat gaagtatcta
    18421 aactggattc aagtacaatt ttaatgtcgg cctctgctgg aggtagaaaa ggtgttcagt
    18481 ttaaagtagt atgtcattct aaggttacgg atggtgaagt aagtgtattg ggagagacaa
    18541 tagttattcg gaatgctaca gaggtatttc tttatctcaa atcaatgacg gattattggg
    18601 gaaatataga tatttcttct cttcagggag aatttagtag tattgattac tttacagaaa
    18661 aagatgaaca tgtaaaaaaa tatcaggagc aatttaatag agttgatttt aaactagact
    18721 atagtaaaga ttgtcttagc attccaacga atctacttct tgaaaacact aaaaagtata
    18781 gtaactactt gactaacttg ttatttcatt atggaagata tctgttaata tcgtctagtc
    18841 aaccgaatgg tttacctgcc aatcttcaag gaatatggtg tgatgaatta aatccaattt
    18901 ggggttctaa atatacgatt aatattaata ctcaaatgaa ttattggatg gtaggtccat
    18961 gtgatttacc agaagtagaa tatccattat ttgatatgct cgaaagaatg agagaaccgg
    19021 gaagactaac cgctaagaaa atgtatggag ctagaggttt tacagcacat cataatacgg
    19081 atggttttgg cgatacggct ccccaatctc atgccatggg ggctgcaatt tgggtattaa
    19141 ctattccatg gttatgtact catatttggg aacactattt atatttccaa gatgagcgta
    19201 ttcttacgga acattttgaa atgataaaag aagcatttct tttctttgaa gattatttat
    19261 ttgaggtgga tggctacttg atgacaggtc caagtgtctc accggaaaat aaatatcgct
    19321 taaaaaatgg tattgaagga aatgcttgtc tatcatctac aattgataat caaattctaa
    19381 gatatttttg tgattcatgc attggcattg caaaacaatt aggagacaat tcggatttta
    19441 ttagtcgtgt gaaggagtta aaaaagaaac tacctaaaac aaaaataggt agtaatgggc
    19501 aaatccaaga atggttagaa gattatgaag aagtagagcc tgggcataga cacatttcac
    19561 ctctatttgg gctttatcct tataatgaga ttgatattca taaaactccg gaattagcag
    19621 aagcagctaa aatcactatc aataggagat tatcaaacgc taatttttta tcttcacagg
    19681 agagggagca agcgattaat aattggttag taagtggttt gcatgctagt acacaaacag
    19741 gttggagtgc tgcatggctg attcattttt ttgcgagact atatcaaggt gaacctgctt
    19801 ataaccagat taatggtttg ttaaataatg cgactcttgg caatttattt cttgaccatc
    19861 caccatttca aattgatggt aatttaggtt tggtgagtgg aatttgtgaa ttattagtac
    19921 agagccatca taattggtta tcactaattc cagctttacc ttctgcttgg tcagaaggag
    19981 aagtgaaagg tttcagagta agaggaggat ataaggtatc gtttgcttgg aaaaatgggg
    20041 atataacatt cctaaaattg gaaggaggaa acaaagatca aaaagtaaga gtaagaatat
    20101 atggcaaaaa tactgatgta caaaatattg aattggtatt taattcagaa aaaattattg
    20161 agttaaattt ttaggtataa gtcatgaata aagaaaaaat aaaaagaaaa ttaatcacaa
    20221 tattgtttgt atgtattggg atgttatgtt ttggattgtt agcaggagtt aaggctgata
    20281 atcgtgttca aatgagaacg acgattaata atgaatcgcc attgttgctt tctccgttgt
    20341 atggcaatga taatggtaac ggattatggt gggggaacac attgaaggga gcatgggaag
    20401 ctattcctga agatgtaaag ccatatgcag cgattgaact tcatcctgca aaagtctgta
    20461 aaccaacaag ttgtattcca cgagatacga aagaattgag agaatggtat gtcaagatgt
    20521 tggaggaagc tcaaagtcta aacattccag ttttcttggt tattatgtcg gctggagagc
    20581 gtaatacagt tcctccagag tggttagatg aacaattcca aaagtatagt gtgttaaaag
    20641 gtgttttaaa tattgagaat tattggattt acaataacca gttagctccg catagtgcta
    20701 aatatttgga agtttgtgcc aaatatggag cgcattttat ctggcatgat catgaaaaat
    20761 ggttctggga aactattatg aatgatccga cattctttga agcgagtcaa aaatatcata
    20821 aaaatttggt gttggcaact aaaaatacgc caataagaga tgatgcgggt acagattcta
    20881 tcgttagtgg attttggttg agtggcttat gtgataactg gggctcatca acagatacat
    20941 ggaaatggtg ggaaaaacat tatacaaaca catttgaaac tggaagagct agggatatga
    21001 gatcctatgc atcggaacca gaatcaatga ttgctatgga aatgatgaat gtatatactg
    21061 ggggaggcac agtttataat ttcgaatgtg ccgcgtatac atttatgaca aatgatgtac
    21121 caactccagc atttactaaa ggtattattc ctttctttag acatgctata caaaatccag
    21181 ctccaagtaa ggaagaagtt gtaaatagaa caaaagctgt attttggaat ggagaaggta
    21241 ggattagttc attaaacgga ttttatcaag gactttattc gaatgatgaa acaatgcctt
    21301 tatataataa tgggagatat catattcttc ctgtaataca tgagaaaatt gataaggaaa
    21361 agatttcatc tatattccct aatgcaaaaa ttttgactaa aaatagtgag gaattgtcta
    21421 gtaaagtcaa ctatttaaac tcgctttatc caaaacttta tgaaggagat gggtatgctc
    21481 agcgtgtagg taattcctgg tatatttata atagtaatgc taatatcaat aaaaatcagc
    21541 aagtaatgtt gcctatgtat actaataata caaagtcgtt atcgttagat ttgacgccac
    21601 atacttacgc tgttgttaaa gaaaatccaa ataatttaca tattttattg aataattaca
    21661 ggacagataa gacagctatg tgggcattat caggaaattt tgatgcatca aaaagttgga
    21721 agaaagaaga attagagtta gcgaactgga taagcaaaaa ttattccatc aatcctgtag
    21781 ataatgactt taggacaaca acacttacat taaaagggca tactggtcat aaacctcaga
    21841 taaatataag tggcgataaa aatcattata cttatacaga aaattgggat gagaataccc
    21901 atgtttatac cattacggtt aatcataatg gaatggtaga gatgtctata aatactgagg
    21961 ggacaggtcc agtctctttc ccaacaccag ataaatttaa tgatggtaat ttgaatatag
    22021 catatgcaaa accaacaaca caaagttctg tagattacaa tggagaccct aatagagctg
    22081 tggatggtaa cagaaatggt aattttaact ctggttcggt aacacacact agggcagata
    22141 atccctcttg gtgggaagtc gatttgaaaa aaatggataa agttgggctt gttaaaattt
    22201 ataatcgcac agatgctgag actcaacgtc tatctaattt tgatgtgatt ctatatgaca
    22261 ataatagaaa cgaagttgct aagaaacatg ttaataattt gtcgggtgaa tctgttagtc
    22321 tagatttcaa agaaaaagga gcaaggtata ttaaagttaa attactaacg agtggagtgc
    22381 ctttgagttt agcagaagta gaggttttta gagaatcaga tggtaagcaa tctgaagagg
    22441 atatagataa aataacagaa gataaagtag tctctacaaa taaggtagct actcaaagtt
    22501 caaccaatta tgagggtgta gctgctttag cagttgatgg taataaagat ggagattacg
    22561 gacatcattc ggtgactcat actaaggcag attctaacgc ttggtggcag gtcgatctgg
    22621 gagaagagtt tacggtttct aaagttgata tttataatag aacagatgcc gaacctcagc
    22681 gtttatctaa ttttgatgtt atttttctat cttcatcagg agaagaagtt tttagaagac
    22741 attttgataa agtagttgat ggtttgttat ctttaaaagt accttctgta ggggctaagc
    22801 tagtcaaaat agaattaaaa tcagcagcta ttccgttaag tttagcggaa gttgaagtct
    22861 atggttcaaa gagaactccg aagaaacttt ctaatattgc attaacaaaa gaaactcgac
    22921 agagttcaac ggattacaat ggtttttctc gtctagcagt tgatggaaat aaaaacggag
    22981 attatggtca tcattcagtg actcatacca aagaagattc tccttcatgg tgggagatag
    23041 atttagcaca aaccgaagaa ttagaaaagt taattattta taatagaaca gatgctgaaa
    23101 ttcagagatt atcaaatttt gatattatta tatatgattc aaatgattat gaagttttta
    23161 cacaacatat tgacagttta gaaagcaata atctatccat agacttaaaa ggactgaagg
    23221 gaaaaaaggt tagaatttct ttgagaagcg caggaattcc tttaagttta gcagaggtag
    23281 aggtttatac ttataagtaa ttttaaaaat tatcacccag gctaccgtaa atataatgga
    23341 gatggtagta tgaaagaaac agaaaaataa gaggaaaata gtatgattca acatccacgt
    23401 attgggattc gtccgactat tgatggtcgt cgtcaaggtg tacgcgaatc acttgaagtg
    23461 caaacaatga acatggctaa aagtgtggca gatttgattt caagcacatt gaaatatcca
    23521 gatggggaac ctgtggaatg cgtgatttct ccatctacta ttggccgtgt accagaggct
    23581 gcagcttccc atgagttgtt taaaaaatca aatgtttgcg caacaattac agttacacca
    23641 tgctggtgtt atggtagtga aactatggat atgtctccag atattcctca tgctatttgg
    23701 ggatttaatg ggacagaacg cccaggagct gtctatcttg cagctgtact agcttcacat
    23761 gctcaaaaag ggattccagc ctttgggatt tatggaagag atgttcagga agctagtgac
    23821 acagatattc cagaagatgt caaagaaaaa cttttacgct atgcgcgtgc agctcttgca
    23881 actggcttga tgagagacac tgcttaccta tcaatgggta gtgtttcgat ggggattggt
    23941 ggttctattg taaatccgga tttcttccaa gaatacctag gaatgcgaaa tgaatcggta
    24001 gatatgacgg agttcacgcg ccgtatggac cgtagtattt acgaccctga agagttcgaa
    24061 cgtgcgctca aatgggtgaa agaaaacgta aaagaaggat tcgaccataa ccgtgaagac
    24121 cttgttttaa gccgtgaaga aaaagataga caatgggaat ttgttattaa gatgttcatg
    24181 attggacgtg acttaatggt tggtaaccca agacttgctg aacttggttt tgaggaagaa
    24241 gcagttggtc accatgcttt agtagctggt ttccaaggtc aacgtcagtg gacagaccat
    24301 tttccaaatg gagattttat ggaaactttc ctcaatactc agtttgactg gaatggtatt
    24361 cgaaaaccat ttgtatttgc gacagagaat gattcactaa atggtgtgtc tatgctcttt
    24421 aattatctat taacaaatac tccacaaatc tttgctgatg tgcgtactta ttggagccca
    24481 gaggctgtta aacgtgtaac gggacatact ttagagggtc gtgctgcagc tggcttctta
    24541 catctaatca actctggttc ttgtacattg gatggtacag gtcaagctac tcgagatggc
    24601 aaacctgtta tgaaaccatt ctgggagttg gaagaaagtg aagtgcaggc tatgcttgaa
    24661 aatacagact tcccaccagc aaaccgcgaa tacttccgtg gaggaggatt ctcaactcgt
    24721 ttcttgacga agggggatat gccagtaaca atggtacgtc tcaatcttct aaaaggggtt
    24781 ggtccagtgc tacaaattgc agaaggttat acacttgaac ttcctgaaga tgttcaccat
    24841 actttagata atcgtacaga tccaggatgg ccaactactt ggtttgctcc acgtttgaca
    24901 ggaaaaggtg ctttcaagtc tgtctatgac gtcatgaata attggggagc taatcacgga
    24961 gccataacat atggacacat tggagcagac ttgattacct tggcttctat gttgagaatt
    25021 cctgtcaata tgcataatgt acctgaggaa gatatcttta gacctaaaaa ttggtcctta
    25081 tttggaacag aagatctaga atcagcagac tattgtgcat gtcagttgtt gggaccacta
    25141 cataaataaa acttgtttat ataggaggtg aacttacgtc cctcctatcc ttttaaaaag
    25201 atttgttaaa caattcacaa ataattaaaa acgaatacaa aaagtaatat aatgatgtta
    25261 aatagatagc gcggaggcgc aggaggaaaa ttatatggct acattttatg ttccggcagt
    25321 caaccttatt ggaaaaggtg ttgtaaatga agtgggtcct tatatcaagg aacttggcta
    25381 taaaaaggca cttttggtta cagataagta catcgaaggc agtgatattt tacctaagac
    25441 tttaaaacca ctggatacag aaggaatcga atatgtcatc tttagcgatg tagagccaaa
    25501 ccctacttgt aaaaatgtca cagatggggt agctgctttg caagaacatg gctgtgactt
    25561 tatcatcagt cttggcgggg gttctccaca ggatgcagct agttgtattt ctatcatggc
    25621 tacaaatggt ggaaaaccac aggattatga agggcttcat aagtctgcta aaaaaggctt
    25681 gccagttgtg gctatcaata caacggcagg aacttctgca gaaatcacta ttaactatgt
    25741 gattactgat gaagaacgca aggttaagat ggtaatggtt gacaagaata gccttgctct
    25801 tatctctgtt aatgacccgg aactcatgct ttccaaacct aaaggcctga ctgctgctac
    25861 tggtatggat gctctgactc acgctgttga agctttggta acacctggtg cttatgatgt
    25921 aaccaagaaa ctgtctattg gtgctattga gcttatcaag gaatatcttc ctcgtgctgt
    25981 agaaaatgga catgatattg aagcgcgtga aggtatggtc aatgccatct tccttggtgg
    26041 tatgagcttt aataatgctg gtcttggtta tgttcactca atggctcacc aactcggtgc
    26101 agtatataat ttgccacatg gcgtgtgctg tgccatgttg ctaccagtta tagaacgtga
    26161 aaatgctaaa cgtgtaccag aagctttccg caatgttgcc aaagccttgg gacttcatgt
    26221 agaaggtaaa tcagatcaag aatgtgccga ttatgcgatt gctgagattg agaagctttc
    26281 tgagacagta ggtattccta agaaactgac tgaacttggt attgaagaaa aagatttcga
    26341 ctttgaatac ctttctaaga atgccttgat tgatgcctgc gcaccaggaa atccatttat
    26401 gccaacctta gaagaaacga ttgcctttta taaagagtta ttttagcaag agattggacg
    26461 acaataggac aattcacctc taattattga catttcagga aaaatcgcag taaaaacggc
    26521 acagccttct ttaaatgtgc tataatacta gaaaaatact tgtggaggtt ccattatggc
    26581 aatatttttc atgatttttc tgattgtttg tgtgctccta ttggtgatag tcacactgag
    26641 tacagtttat gtggttcgtc agcagtcggt ggcgattatt gaacgctttg ggaaatacca
    26701 aaaggttgct aatagcggta ttcatattcg cttgcctttt gggattgact cgattgcagc
    26761 acggattcag ttgcgcttgt tgcaaagtga tattgtggtt gagactaaga ccaaggacaa
    26821 tgtgttcgtt atgatgaatg tagcgactca gtaccgtgtc aacgagcaga gcgtgacaga
    26881 tgcttactat aaactcatac gtccagaatc tcagattaaa tcttatatcg aagatgctct
    26941 tcgctcttct gttccaaaat taaccttgga tgaattgttt gagaaaaaag atgagattgc
    27001 ccttgaggtt caacaccaag tagcagaaga aatgaccact tacggctaca ttatcgtgaa
    27061 aaccttgatt accaaggtcg aaccagatgc agaagttaag caatccatga atgaaatcaa
    27121 tgcggcgcaa cgtaagcggg tcgcagcaca agaattggcg gaagctgaca agattaaaat
    27181 tgtcactgca gctgaagcag aaaaagaccg ccttcatggt gtggggattg cccaacaacg
    27241 taaggcgatt gtggatggat tggcagagtc tatcaccgaa ctcaaggaag ccaatgttgg
    27301 catgacagaa gaacaaatca tgtctatcct cttgaccaac cagtatttgg ataccttgaa
    27361 tacctttgcc tctaaaggaa atcaaaccat ctttttacca aatactccaa atggtgtgga
    27421 tgatatccga acacaaatct tgtcagccct tcgcgctgag aagaaataat agactaatac
    27481 tcttcgaaaa tctcttcaaa ccacgtcagc gtcgccttgc cgtatagatg ttactgactt
    27541 cgtcagttct atctgcaacc tcaaaacagt gttttgagct gacttcgtca gtcttatcta
    27601 ctacctcaaa acggtgtttt gagcaacctg tggctagttt cctagtttgc tctttgattt
    27661 tcattgagta taaagagctt ggcaacaggc tctttttgta ttgcgtttat taagaaaaac
    27721 aaaaaacatc gatatacatt taactaaaaa ttattgtatg tcatctccct taattaggaa
    27781 tgataaggga ataactagaa agatttgtga atacaaacta tttctgatat acaaaatata
    27841 cagtaataat gaatgatggg agatgggatg aaagaatttc aatttgagag aaagcagcgt
    27901 ttttctttga ggaaatatgc aataggagct tgttcggtct tgctaggaac gagtttattt
    27961 tttgctggta tgggtgctca gcctgtacag gatacagaaa cgagttcagc actaatttca
    28021 agtcattatt tggatgagca ggatttatct gaaaagctga aatctgagtt gcaatggttt
    28081 gaattagaaa acaagctttt gaacttatgg gagcattagg ttactatgaa ggatttgttc
    28141 cttatgtttc aaatcaatac aaaaaccaag ctgaagaaga atacaaaccg ctatctgata
    28201 aatatatttt cgaaaaaatc ttaggaaaaa catatgcagc cttcaaaaaa gatcaaatta
    28261 atgagcgtgt cgagaaatta ggtaagttaa aacctattac aataaattac aacggaaaat
    28321 cagaagtaat tgatagtaaa gaaaaattac aagagcttat gaataaagcc gttaaagacg
    28381 aagtggctca aatatagtaa aatgaaataa gaacaggaca aatcgatcag gacagtcaaa
    28441 tcgatttcta aaaatgtttt agaagtagag gtgtactatt ctagtttcaa tatactataa
    28501 aatcaggcaa taccacggct aaaaagtttg agtttatcga aacaccagtg caaaaactga
    28561 aaaaagcgat ttataaagct catctcaaag attcagatga ctttagacca gaaacatcca
    28621 caccaaatct ttttgaaagc tgtttgaag
//
LOCUS       DBEROU010000027        28012 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.027, whole genome shotgun sequence.
ACCESSION   DBEROU010000027 DBEROU010000000
VERSION     DBEROU010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 28012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28012
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.027"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(8..1396)
                     /gene="mobQ"
                     /locus_tag="ACL8CL_001263"
                     /pseudo
     CDS             complement(8..1396)
                     /gene="mobQ"
                     /locus_tag="ACL8CL_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785367.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
     gene            complement(1710..2027)
                     /locus_tag="ACL8CL_001264"
     CDS             complement(1710..2027)
                     /locus_tag="ACL8CL_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000968321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3847 domain-containing protein"
                     /protein_id="HGS1330513.1"
                     /translation="MMASKRLSIEEQIEKKEESIKQLQNQKRQLKKKLNEQERKARNK
                     RLIEKGAVFESIFEESIDLTKDEFYKLIKTLNDEEIRLNIMEILEERIDDNVEKSSKD
                     EIT"
     gene            complement(2083..2508)
                     /locus_tag="ACL8CL_001265"
     CDS             complement(2083..2508)
                     /locus_tag="ACL8CL_001265"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1330514.1"
                     /translation="MPKRYLLDGKTPRISATNIDNGILGYYEDIDDKNYRVFENFISV
                     SFLGAVFYHKYKASLDMKIHCLKLKNKELNKEVAFYLTSIIRQALKNTEYKDQISSTV
                     LPDIKIKLPIDSRGTPDWNYMERYIEDLKLKCNIANYNI"
     gene            complement(2561..2745)
                     /locus_tag="ACL8CL_001266"
                     /pseudo
     CDS             complement(2561..2745)
                     /locus_tag="ACL8CL_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612340.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2896..3690)
                     /locus_tag="ACL8CL_001267"
     CDS             complement(2896..3690)
                     /locus_tag="ACL8CL_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330515.1"
                     /translation="MKGLWSNNLTCGYDEKIILENINIKIPEEKISVIIGSNGCGKST
                     LIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPIIPESITVADLVSRGR
                     FPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQRQRVWIALALAQDTSI
                     LLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLTARYADYLFAIKEGKL
                     VAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHHINS"
     gene            complement(3690..4697)
                     /locus_tag="ACL8CL_001268"
     CDS             complement(3690..4697)
                     /locus_tag="ACL8CL_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HGS1330516.1"
                     /translation="MQNLIIGIQKRKNRITLFSSLFLLIIISLSFFILLIGDESYSFS
                     TLIKVLNSETVPGASFSIMEIRLPKLLAGIIAGWSFGLAGFIFQTMLRNPLASPDIIG
                     VTSSSSIAAVFCILVLKTNSLTTGIISITCGLTSSLILFLLAKKDGFSAARLIILGIG
                     FQAVTRAGTSFLLLKVARYELQEVMRWLSGSLSFTKLDDIPLVLIVSIIATILVLFFN
                     KRLEIIELGEEIAIGLGANPELSRLVLIFCAVSLTAFSTSITGPIACISFLAGPIALN
                     IGKKRSPILAGLVGILLVLLSDIFSQNILPARYPVGVVTGLLGSPYLIYLLIKMNRRN
                     I"
     gene            complement(4697..5704)
                     /locus_tag="ACL8CL_001269"
     CDS             complement(4697..5704)
                     /locus_tag="ACL8CL_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HGS1330517.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTMKNRGVN
                     N"
     gene            complement(5694..6719)
                     /locus_tag="ACL8CL_001270"
     CDS             complement(5694..6719)
                     /locus_tag="ACL8CL_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000790748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HGS1330518.1"
                     /translation="MKNKFFLIAILAMCIVFSACSSNSVKNEENTSKEHAPDKIVLDH
                     AFGQTILDKKPERVATIAWGNHDVALALGIVPVGFSKANYGVSADKGVLPWTEEKIKE
                     LNGKANLFDDLDGLNFEAISNSKPDVILAGYSGITKEDYDTLSKIAPVAAYKSKPWQT
                     LWRDMIKIDSKALGMEKEGDELIKNTEARISKELEKHPEIKGKIKGKKVLFTMINAAD
                     TSKFWIYTSKDPRANYLTDLGLVFPESLKEFESEDSFAKEISAEEANKINDADVIITY
                     GDDKTLEALQKDPLLGKINAIKNGAVAVIPDNTPLAASCTPTPLSINYTIEEYLNLLG
                     NACKNAK"
     gene            complement(7112..7330)
                     /locus_tag="ACL8CL_001271"
     CDS             complement(7112..7330)
                     /locus_tag="ACL8CL_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1330519.1"
                     /translation="MALNYKPLWIQLAKKGLKKTDVIAMAGLTTNVMAQMGKDKPITF
                     KNLERICKALSCTPNDIISFEDNFSDEE"
     gene            complement(7576..7854)
                     /locus_tag="ACL8CL_001272"
     CDS             complement(7576..7854)
                     /locus_tag="ACL8CL_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330520.1"
                     /translation="MKKDKEVYDKVCEYLSPHLNKDMEVFELACWFRVITVIEANSYV
                     NIRSSRLLPKFKTQKINSWCAAYNIHFNNGYCKQHTNEFKDSLKSLIL"
     gene            complement(8068..9690)
                     /gene="rlmD"
                     /locus_tag="ACL8CL_001273"
     CDS             complement(8068..9690)
                     /gene="rlmD"
                     /locus_tag="ACL8CL_001273"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGS1330521.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKDS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVTTRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIELREH
                     YNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(9764..10738)
                     /locus_tag="ACL8CL_001274"
     CDS             complement(9764..10738)
                     /locus_tag="ACL8CL_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HGS1330522.1"
                     /translation="MRKKLFLTSAAILWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGMEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDVRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(10812..11426)
                     /gene="pmp23"
                     /locus_tag="ACL8CL_001275"
     CDS             complement(10812..11426)
                     /gene="pmp23"
                     /locus_tag="ACL8CL_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HGS1330523.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNRTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(11426..12400)
                     /locus_tag="ACL8CL_001276"
     CDS             complement(11426..12400)
                     /locus_tag="ACL8CL_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HGS1330524.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(12408..13664)
                     /gene="glyA"
                     /locus_tag="ACL8CL_001277"
     CDS             complement(12408..13664)
                     /gene="glyA"
                     /locus_tag="ACL8CL_001277"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HGS1330525.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVETLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMSLIEPGDTVMGMDLASGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITARGFGEEESRKVAALIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYED"
     gene            complement(13726..14157)
                     /locus_tag="ACL8CL_001278"
     CDS             complement(13726..14157)
                     /locus_tag="ACL8CL_001278"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014947335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1330526.1"
                     /translation="MLRDLQETDVKAICDINQEALGYTFSPEETASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEEEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIC"
     gene            complement(14159..14758)
                     /locus_tag="ACL8CL_001279"
     CDS             complement(14159..14758)
                     /locus_tag="ACL8CL_001279"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HGS1330527.1"
                     /translation="MDRIRQELENGGAVVLPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNIASFEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNSDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEA"
     gene            complement(14745..15584)
                     /gene="prmC"
                     /locus_tag="ACL8CL_001280"
     CDS             complement(14745..15584)
                     /gene="prmC"
                     /locus_tag="ACL8CL_001280"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HGS1330528.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQFVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPAWSVTAADVSQEALELASENASNQNLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVGLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQNRMVVVDD
                     GQD"
     gene            complement(15584..16663)
                     /gene="prfA"
                     /locus_tag="ACL8CL_001281"
     CDS             complement(15584..16663)
                     /gene="prfA"
                     /locus_tag="ACL8CL_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HGS1330529.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEELAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(16660..17250)
                     /locus_tag="ACL8CL_001282"
     CDS             complement(16660..17250)
                     /locus_tag="ACL8CL_001282"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HGS1330530.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            17373..17555
                     /locus_tag="ACL8CL_001283"
     CDS             17373..17555
                     /locus_tag="ACL8CL_001283"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HGS1330531.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            17691..19064
                     /gene="mnmE"
                     /locus_tag="ACL8CL_001284"
     CDS             17691..19064
                     /gene="mnmE"
                     /locus_tag="ACL8CL_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HGS1330532.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTDSFAIAQKIFKGKDL
                     SQVASHTLNYGHIIDPLTGKVMDEVMVGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNIRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTKLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(19111..20046)
                     /gene="dapA"
                     /locus_tag="ACL8CL_001285"
     CDS             complement(19111..20046)
                     /gene="dapA"
                     /locus_tag="ACL8CL_001285"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HGS1330533.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLAHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKVVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLIYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFTAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAILNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(20102..21178)
                     /locus_tag="ACL8CL_001286"
     CDS             complement(20102..21178)
                     /locus_tag="ACL8CL_001286"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HGS1330534.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGIIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPCDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(21521..21593)
                     /locus_tag="ACL8CL_001287"
     tRNA            complement(21521..21593)
                     /locus_tag="ACL8CL_001287"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21558..21560),aa:Thr,seq:ggt)
     gene            complement(21693..22202)
                     /locus_tag="ACL8CL_001288"
     CDS             complement(21693..22202)
                     /locus_tag="ACL8CL_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HGS1330535.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(22200..22296)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            22325..22756
                     /locus_tag="ACL8CL_001289"
     CDS             22325..22756
                     /locus_tag="ACL8CL_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HGS1330536.1"
                     /translation="MKIPIQKFFNNEILAYLFFGIVTTLVAILLRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKVYIFRK"
     gene            22838..23215
                     /gene="mscL"
                     /locus_tag="ACL8CL_001290"
     CDS             22838..23215
                     /gene="mscL"
                     /locus_tag="ACL8CL_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HGS1330537.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKEE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(23257..24351)
                     /gene="hemH"
                     /locus_tag="ACL8CL_001291"
     CDS             complement(23257..24351)
                     /gene="hemH"
                     /locus_tag="ACL8CL_001291"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HGS1330538.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGMRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRPPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESQDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            24506..25729
                     /gene="pepT"
                     /locus_tag="ACL8CL_001292"
     CDS             24506..25729
                     /gene="pepT"
                     /locus_tag="ACL8CL_001292"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HGS1330539.1"
                     /translation="MTYPNLLDHFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTTLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEV
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(25765..26418)
                     /locus_tag="ACL8CL_001293"
                     /pseudo
     CDS             complement(25765..26418)
                     /locus_tag="ACL8CL_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672614.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family hydrolase"
     gene            26416..26580
                     /locus_tag="ACL8CL_001294"
                     /pseudo
     CDS             26416..26580
                     /locus_tag="ACL8CL_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057566.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(26657..27300)
                     /locus_tag="ACL8CL_001295"
                     /pseudo
     CDS             complement(26657..27300)
                     /locus_tag="ACL8CL_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672611.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
     gene            complement(27407..27967)
                     /locus_tag="ACL8CL_001296"
                     /pseudo
     CDS             complement(27407..27967)
                     /locus_tag="ACL8CL_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         8134 a         5529 c         4855 g         9494 t
ORIGIN      
        1 caagatttta cattaaaaga attttttctt cattccactt tttaggactt agtttttcct
       61 tatctgatag gagttttttt aatttcccgc ttactacttt atattggtct atatctttct
      121 tatgtttgtt ataaaatgct tcttttgtga atatattttt cttttgatat tcttcataaa
      181 cttctttttt ggctttgtag atttcatagt atagaatttt cttttctaca tcttccattt
      241 ctttatggat tgtctttgct tttttattta gcctataact attagacttt aaagtttcta
      301 tttttgtttg taactgacct atggtttcta tgttgttttt tcttaaataa ctatatgcac
      361 ttgttagctt tttgaagtca tatttttctt tattagtttt tgcatatcct tttagatatt
      421 tacttttttc tttttgaatt tctgaataag ttaatagata atttgttaga ttaaaaagtt
      481 tagggttgtc tattacctta tctctttcga tatccttaaa tttttcataa gcagtagata
      541 agttatctaa taaatttcct atccaaccct ttaatgtttt tatctcttct tttattgttt
      601 ttacaagttg attatatttt cttatttctc tattatagtt tcccttgtca gtttctattc
      661 cctttctttc cattgcactt gctgctgatc ctaaatggat tgtcggtaga taatctgaat
      721 tttatctttt aaaactccta tggtctactc ttttttctgc tcctgctctt tctaaatatt
      781 tgttacaaag atctgaaaaa ttttctctcc atttttctac attgcctgta tcgttccagg
      841 ttgttagttc tacttttctt gtctttggtt ttccattttt gtttaatact ttttctcctt
      901 tttcatctag gatatattct ttttttgact tggctaagaa ctctcctttt tcgtttattg
      961 gtcgcattat ggtcattata tgacaatgga tatttccatt tttatcttga ctctcatcat
     1021 gaattgcata atctactatc attccttgtg atgttaaatt ttcttttata tatctttcaa
     1081 ccaggttttt attttcactt aaagataact cttttggtag tcctattatg aattgtcttg
     1141 ctagttgtgc attagaattt ttttctgcca tttctacttt attccataag atagacctat
     1201 cattaaattc ttttggtata tgatcaggca atattatatt ttttactaat attttttctt
     1261 ttcttgtgta gtcatgggtt actccgtccc attcattttt tattttttct ccacttatat
     1321 atgctgcact tgctactgca cttttgcctt ttcctcttga tattatgttt actgaaaaat
     1381 gaaaactgtc tgccattttt atcacttcct ttctttcttt gttgttttag gtggaggctt
     1441 aatagttttt ttatatcctc tttgtgaatg agcgttttga aatgatagaa ttaaaaaagc
     1501 cgactactga attaattttt gttgtttcag tatgtcggct taattgttac tttcatttca
     1561 aattttaaaa gtcaagggcg agccttagcg agtttattta cccttgactt ttaaaaagcg
     1621 aatggttgtt gctccctgca agggtttgac tccgcaggac gcaagtaccc tttagggtgt
     1681 ataattgcgc ccttgtaacc aagggacaat tatgttattt catctttaga tgatttttct
     1741 acattatcat ctattctttc ttctaaaatt tccattatgt ttaatcttat ttcttcatca
     1801 tttaacgttt ttattaactt ataaaattca tcttttgtta aatcaatact ttcttcaaaa
     1861 atactttcaa acactgcacc tttttctata agtcttttgt ttcttgcttt tctttcttgt
     1921 tcattaagtt ttttcttcaa ctgtcttttt tgattttgta attgcttaat gctttcttct
     1981 ttcttttcaa tctgttcttc aatacttaat cttttacttg ccatcatgta tcctttctta
     2041 aaataaaaaa cgactagatt tttatttcta atcgttcttg ttttaaatat tataatttgc
     2101 tatattgcat tttaatttta agtcttctat atatctttcc atgtaattcc aatctggagt
     2161 acctctactg tctatcggta atttaatttt gatatctggc aatactgtgc tagatatttg
     2221 atctttatat tcagtatttt ttaaagcttg cctaataatt gaagttaaat agaatgctac
     2281 ttctttattc aattctttat tttttaattt taaacaatga attttcatat ctaggcttgc
     2341 tttatattta tgataaaaga ctgctcctaa aaatgaaaca gatataaaat tttcaaagac
     2401 tctataattt ttatcatcta tatcttcgta gtaacctaag ataccattgt caatatttgt
     2461 agcactaatt ctaggagttt ttccatcgag taaatatctt tttggaatag atgagcctgt
     2521 tggtacttaa ctcgatttcc cctttttttc ggtaataaat atttcttttt attttgttgt
     2581 ctgatatttt tcctacctgt cctttgtagg atgagtattt tctagatttt cttgaataac
     2641 tttttacttg aagttttagc ttttgaacta gtcgttgtac tttctttttg tttattatca
     2701 gtcctgatct ttttaatatt gctgttattc tctatatcct atttttcatt catgatattc
     2761 ttttactaat tttatcttaa attctgtgct gtatttgcca ttaaaaaact gacctccttt
     2821 agttagtttt ttggcctaac ttttgagggt cagttcaaaa tttgcgactt ttaaatgaat
     2881 tccaatattc aattattaag agttaatatg gtgcttgcca ataggaatca ttagaggcga
     2941 attggaaata gggtcacgta taatttttgc ttcaagatta aagatatctt taactagttt
     3001 atcatttagt atatcttcag gctttccctc tgcaacaagt ttaccttctt taattgcaaa
     3061 taggtaatca gcgtatcttg ctgttagatt tatatcgtgc aaaatcatgc aaatggttgt
     3121 cttatatttt tggtttagat cagtcaagag gtctaatagt tctatttgat atgagatatc
     3181 caagtaagta gttggctcat ctaaaagtag gatacttgta tcttgggcta gggctagagc
     3241 tatccatact ctttgccttt gacccccaga aagttcttca actaggttat ttgctagatc
     3301 ttcaacattg gccttaacca ttgatctgtt tattatttca aggtcatctt ttccaagact
     3361 cttaaaaggc tttctgtagg ggaaacgacc acggcttaca agatcagcta ctgttattga
     3421 ttcagggatt attggagatt gaggtaatat agctatgtgt tttgctaaat ctttttcttt
     3481 ataagaatta attgatttat tatcaagcaa tacttctccc tctaatggct ttataagtcg
     3541 agacaaggtt ttaatgagtg ttgatttccc acaaccattt gacccaataa taactgatat
     3601 tttttcttca ggtattttta tatttatatt ttccaagatt attttttcat cataaccgca
     3661 ggtaagatta tttgaccaca gacctttcat tatatattcc tcctgttcat ttttattagt
     3721 aagtatatta aatatggtga acctaacaag ccagttacaa cacctactgg atatctagct
     3781 ggtaaaatat tttgagagaa tatgtctgat aacaaaacta gtaaaattcc aaccaatcca
     3841 gctaatattg ggcttctttt cttgccaata tttaaggcta tgggaccagc taaaaaagat
     3901 atacaagcta ttggtcctgt aattgaagta gaaaaagcag ttaaagatac agcgcaaaaa
     3961 attaaaacaa gccttgaaag ctcgggattt gctccaagtc cgattgctat ttcttcacca
     4021 agttcaataa tttctagtct tttattaaaa aataaaacta atatagtagc aataatactt
     4081 actattagaa caaggggtat gtcatctaac tttgtaaaag ataaagagcc actgagccat
     4141 ctcataactt cttgtaattc atatcttgct actttcaaca ataaaaatga ggtgcctgct
     4201 cttgtgacag cttgaaaacc aatacctaat attatcagtc ttgctgctga aaaaccatct
     4261 tttttagcta gtaaaaataa tattaaagat gatgttagtc cacaagttat tgaaataatt
     4321 ccagtagtta aactatttgt ttttaatacc aatatgcaaa agaccgctgc aatagatgaa
     4381 gaacttgtga caccgattat atcaggactt gcaagaggat ttcttaacat agtttgaaag
     4441 ataaatcctg ccaatccaaa agaccagcca gctataattc ctgctaataa ttttggtaat
     4501 ctaatttcca taatcgaaaa actagctcca ggaacagttt cactatttaa gactttaatc
     4561 aaagttgaaa aagaataact ttcatctccg ataagtaaaa tgaaaaatga tagactgatt
     4621 attattaata aaaatagtga ggaaaatagt gttattctat tttttctttt ttgaatacct
     4681 ataattaaat tttgcattag ttattaaccc ctctattttt catagttaca taaataagta
     4741 ctggaccccc gattattgca gtaattatcc ctacttcaat ttcacctggt ttacctaaca
     4801 tacggccgat tatatcacat ataagcaaga gctctgcacc tataaaagat gaagaaatgg
     4861 tcattgtgcg tatatctttg cttataaata agccacaaaa gtgaggaact ataagaccta
     4921 cgaagccaat aggtccacca attgcagtaa tacttgaaca taaaagcaca cttgcaatta
     4981 ttgcaagtga tcttatccta ttaacattaa ctccaagacc aacagccatt tcatcaccca
     5041 tagctaaagc gtttaaatct gatgaaataa atatagctat caagtgacct aaaattataa
     5101 aaggtagtag tgtagatata gaagataatg tagctgctcc aaggctacct atttgccaaa
     5161 atctaaattt gtctaagacg ttattattcg gtaaaattaa aaaacttaca aaactgctta
     5221 aagccatact aacacaagtt cctgataagg caagttttat aggggtaagg cctgcttttc
     5281 cgcttacagc aatcgcgtat acaaaaattg cacttactaa gccaccaatg attgcaaaac
     5341 ttatatggct tatgcttgat gaaattccta aaaaagaaag accaattact acactaagac
     5401 ttgctcctgt gtttataccg agtatacctg gatcagctat tgggtttcta gtaactgatt
     5461 gcatcaatac accgcttatg gcaagactag aacctgctaa aattgcaaaa atagttctag
     5521 gtattctttt atatataatt gatttaataa aatcatcatt tgtattaccc attccaaaag
     5581 ctgctaaaaa atctctgata ttaatttctt ttgttccaag ctttaatgac aaaaatattg
     5641 taataagaag acctaggcag attacaaaaa ttatgcctag gtttatttgt tttttatttc
     5701 gcatttttgc atgcatttcc taaaagattt aggtattctt caatagtata gtttattgaa
     5761 agtggtgttg gagtgcatga ggctgctaac ggtgtattat ctggaattac agcaacggca
     5821 ccatttttaa ttgcatttat tttacctaaa agaggatctt tttgtaaagc ttcaagagtt
     5881 ttatcatcac cataagttat gattacatca gcatcattta tcttatttgc ttcttctgca
     5941 gaaatttcct ttgcaaaact atcttcactc tcaaattctt ttaatgattc agggaaaact
     6001 agacctaaat ctgttaaata atttgctctt ggatctttgc tagtataaat ccagaatttt
     6061 gatgtatctg cagcattaat catagtaaat aatacttttt ttcctttgat ttttcctttg
     6121 atttctggat gtttttctaa ttctttggat atacgagctt cagtattttt gattaactca
     6181 tcaccttctt tttccatacc taaggctttt gaatcaattt taatcatatc tctccataaa
     6241 gtttgccaag gtttagattt gtatgctgct acaggagcaa tttttgatag agtgtcataa
     6301 tcttctttag ttataccaga ataacctgct aagataacat ctggtttaga atttgatatt
     6361 gcttcaaagt taagtccatc caaatcgtca aataggttag ctttaccatt tagttctttg
     6421 attttttctt ctgtccatgg taaaactcct ttatcagcac ttacaccgta atttgctttt
     6481 gaaaatccaa caggaactat tcctaaagct aatgctacat catgatttcc ccaagcaata
     6541 gttgcaactc tttcaggttt tttatctaat atagtttgac cgaaagcatg atctaaaact
     6601 attttatcag gcgcatgctc tttagaagta ttttcttcat ttttaacaga attagaagaa
     6661 caagcgctaa aaactataca catagctaaa atagctatta gaaaaaattt gtttttcata
     6721 aaaactcctt aaacatattt caagtctatt gtatcgataa taattatcat tgtcaataga
     6781 tgcagttaca aatttaataa tataattttt tcaaaaaagg atctagtttt tatttttagt
     6841 taaattatgt ctatattttt cattaactca tcaataaaaa tttgtttgct aatatctctt
     6901 tcttctgatt ttgtttcaag gattattgct acatcattca tatttttagg taaataccaa
     6961 ccatctggct tatctgaata tcccttgaag cctaattgat taaaagtagt tatttgacca
     7021 gtaccttgat tgatgttttc ttcaacttca ttaaagccta tacttctctt gcataatccc
     7081 taacttgatc ttctgtcctt aaagtcattt tctattcctc gtcactaaaa ttatcttcaa
     7141 aactaataat atcattagga gtgcaagata aagccttaca tattctttct aaattcttaa
     7201 atgtaattgg tttatccttt cccatttgtg ccataacatt tgttgtaagt cctgccatag
     7261 ctattacatc tgttttcttt agtccttttt ttgctaactg tatccataat ggtttatagt
     7321 taagtgccat aatatccctc tttttttctt cattagattt atttaattat agcataaata
     7381 ttgatttcat tcaatcttac attgatttag tgatttgttt tagagaattt gttgtgttat
     7441 tccgtcctta ttttgccata aattgtttta agtgggacct cttatcctta ccatttttac
     7501 ctcctaattt tgagtataaa aaagacgata gagtttttaa tttctatcgt ctgagattta
     7561 catattaaat tttcatcaca aaataagact ctttaaagaa tctttaaatt cattggtatg
     7621 ctgcttacaa tatccattat taaaatggat attgtaagca gcacaccaac tatttatttt
     7681 ttgagtttta aactttggta gaagtcttga actccttata tttacataac tatttgcctc
     7741 tataactgtt atgacacgaa accaacaagc aagttcaaac acctccatat ctttattcaa
     7801 atgaggacta agatattcac aaactttatc ataaacctct ttatcttttt tcataaaagg
     7861 ggcatacaac ttagcaaatt tatcctaaaa ttttttgttg cctttatcaa tcatttcaac
     7921 gctcctttaa atataaaaat gatcctatca atctttatat tgatcctatt ttctaattga
     7981 taccttgcaa gaaaggaata atagctgttc ctataccaag taggaatgta ataaatgcag
     8041 aaagtcatat actgtcattc ttttctatta aatcattttg aagtgtctca aagcatccat
     8101 gatggcttct tctttttcag gtgtacactg tggaataatt tgtttatcct ttttagactt
     8161 gttgtaatgt tctcgtaatt ctattccaca tttctttttt atctgtgcaa tatataatgt
     8221 cgaaactttt aattcaaatt tattccaaac atattctttg atttgagcat atgttgcttt
     8281 gctctccgca cttgtcaaat ccagctcaat ttcaacactt atgtgcttat cgacatcgag
     8341 tttggacaaa agtgctaccg tctcgacgtg atgcgtttga ggaaatagat ccaccggctg
     8401 gactttcttc aattcatatc ccaactcttg gtatagttta atatcacgcg ccatggttgc
     8461 gacattgcag gagatatagg cgatgcgatc ggctcctgtt tgggcgcttg ctttgataaa
     8521 gctttctgtc aagcccttgc gtggaggatc aaccaagata acggttggtt gaatcccatc
     8581 tttaagccaa ttcttcatag cattttcagc tgtgtcacag acatagtggg cgtttgaaat
     8641 attgttcagc tgagcatttt ttttactatt ctcaaccgct tctggaatca gttcaacacc
     8701 gtagacttct ttgacatgct tggcgactga taaaccaatg gttccaatac cagaataagc
     8761 atcaataacc acgtcatctt tttttaactc tgcaaagtca atggctgttt gatagagttt
     8821 ctccgccatt tcagtattga cttggtaaaa ggctgggcca gcgatttgga agtcatttcc
     8881 caacatctgg tccgtaatat agtcttgacc ataaagagtg cgccactcct taccaaaaat
     8941 cgcattggta ttctggtcgt tgatattttg catgacagac acaatctctg ggaactgctt
     9001 gataacttgt tcaatcaatt ggtcaacacg aaaaactttt ggacgagttg tcaccaaaac
     9061 gaccatgatt tgtcctgaat agtgaccacg acgcaccaca agattccgaa tcaatccaga
     9121 ctgttccttt tcgtcataag gttttaaatc aaaacgacgg agcaggtctc gaagagctac
     9181 tacgacttgg tcaatgacag gatcctggat aaagaaatct tcaaggggca tgaggttgtg
     9241 cgaattctta cggaaaaatc ctgtttccaa gacaccattc actcgacgaa cgggcacctg
     9301 cgccttattg cgatacttga ctggatgttc cataccaagc gtttcagcaa cttctacatc
     9361 tgcaattcca gcaatcttgt agagactgtc cttgacttgc ttggttttaa acttgagctg
     9421 ttctggataa gaaaggtgtc ctaaatccgc gattcctgaa cgcaggtaag ccaaatctag
     9481 atcttgatta cggtgtggtg actggacaag gtatttttca acttttccaa agccaatctt
     9541 tttattgacc ttgaggacac gcatgagaat tttttcactc ggtaaagcat tctctacaaa
     9601 aaagaccaaa ccatctacct tggcaactcc tgccccttca tgggtcaaat caacaatttc
     9661 aacttctaca atatcatttt tctttaacat tcttttctct ttctattggc cgacaaaaaa
     9721 gacggcaacg ttacggttac catccattat accatgaaat ttcttaagaa ccaaaactct
     9781 tgaagaagtt gacaagggct tgccagaggg agcttaagaa gttaccaccg tcctctagcg
     9841 ccttatccgc atcaaagtta aggttgatat ttttaaaact gtcgccagct tgtgatacga
     9901 tgctttgttt aaggtcattt agggttttag tgaaatctgc attgctgagg atatcactct
     9961 ttgagagatt caaggcaaaa ttgatgatga tattgatctg gtttcctgtt atgacctgat
    10021 caagtttgta attttttaag gtatcttcaa caatcttgcg gacatcttct tctgtcagat
    10081 ttcccttgct ttctttagct ttggcaagtc ctgacttgat atcagctagg gcaacgttta
    10141 atttattagc atcatagcct gatttgtcct tgttttcagc attgatatct gacaaagcct
    10201 tcaactcttc ttgagccaaa tctttattag cttgtggcac cttggctcca ttagcctcta
    10261 gcgaatagta aatccctgct aaggcacttt ctcctgtaac cggaataggg gctgctacag
    10321 tgattttggc atgttccata cccagcgtta ctgctgcgtt tcggtacata tcctgagtca
    10381 ccttagtgat attttctggt gtttcaatct tgacctcaag tggcgatttg tcacctagct
    10441 tttgaatctt ggctgatgaa tacaactgta agctagagtc attggccaca ttcatgattt
    10501 tagaataaac atcaggtgtc atggtcttga gttctttggt atctgttgag gcattgtagc
    10561 ccagtttttt aagagtttga tttttttggt cttcagatag ggaagaacct aggacatatt
    10621 caggttggac ataggtttca tcgataactt tttgaacatc tgttgctgca tgaacgctat
    10681 tcatagctgt tactgcccac aagatcgcag cgctagtcag aaagagtttc tttctcatag
    10741 ggaattgcct cctttacttc tttagagtaa tatatctatc ttaaagaaaa cttataacaa
    10801 aaacacctgg tctagccaga tgttgaaaag agagtgaaac atttgatgat gtaaaggtta
    10861 agtcgtacct gtctagaata ataatagttt cctccattta catagagttc agcaccgtga
    10921 aaaatggaaa tggggtgaat ataactataa gtctttccag tcctattacc aagcaagggg
    10981 gcaacagtct cacgagagta ctgtttggct agagccaggg tattttcctt gccattttgg
    11041 gcgataaaat cgatataggc aggtccaaaa ttataggctt gaacagctgt ccagatatct
    11101 acacccttct tctgcgccag atagagattg cctgtcagag tttgaatgcc ttgccgaatg
    11161 ctagaggcat tatcattgat ggtgttggtg gaaccacttg cagactcact agactgcata
    11221 acatcgcctt cttttccttt tgtttcagta taaatcatag caagcacaag ctcttcgttt
    11281 gctggggtgt cttgttcact caatatttct cgcaccatgg gttgataggt catgacttgt
    11341 ttgacatctt gatgaacgcg gtaagcttta tagccagcaa aaaggaagac tgctagtaca
    11401 agcactcttc gaattcgttt aaacattatt tactttggat atcctcgata tttttgatta
    11461 agatagagta ggttccattt tcgttttgga taaactcaac agactcggcg tcttgataga
    11521 cgttattggg aacgatgagc tcaattccat ttgataagga gagtttttgg ttttcaaatt
    11581 tctttaattg gcgactggca tcaatttcat caaattgaac aggttctggt acggcttctt
    11641 tgacttggtc aataaagctc aaacgagccg tcagattgtt gtcaaaaagg tcattagcca
    11701 atttctcagg tgacaattca ttgctttctt ctaggttgtt gaaaatagct gatttgacct
    11761 tggattgaaa ttgaaaatca tctgtgttaa aagattcagc aattctctgg gctgtttttt
    11821 ccagttcctt gatagatttt ttaggagaaa tcttaggagc gacagcaaga agattatctg
    11881 aaaaatagtt caaaaaagtc ccgttgtact tgattcgttt ttcaatcagg tgatacttgc
    11941 gactctgaag attgaccacc aaggcctcgt cagcacccgt tccaaatcca ggcaggttat
    12001 tctgagtcag cttgattgga ttatcaactt ctcctccgag gtgggtcaag gtctcccgca
    12061 gggcaattcg caagaaagcg aaatgttcta caccttcttt agaaaattga acaaaaatca
    12121 agtcattggt cttgagattt tcagaaatgc taaactcctc tttccaaaga ttagccagcg
    12181 ttactgatgt ctccaacaaa tcgtctgtaa tatgattgaa gaagggattt tcttcttcga
    12241 aaatcccagt cttggcttca tctgaataca catgttcaat ttttttacgt aggtattctt
    12301 cgatttttgg agtaatattg agaaacttat ctgctaagaa cagctcggta tcatccggac
    12361 tgaactggtg aataatggct ttcttaatat aaatgtccat aaaagtttta gtcctcgtat
    12421 aatgggaagg catctgtcaa ttctttgact gcacttctca cttcttctaa tacagcctca
    12481 ttttctgaat tcttaagggt tttaatgatg agtgcagcca ctttgcgact ttcttcttca
    12541 ccaaatccac gtgcagtaat agctgctgct ccgatacgaa tcccacttgt cttgaatggt
    12601 gacaaggttt cgtaagggat tgaattttta tttagggtaa tattgacttc atccagcaag
    12661 ttttgagcaa cttttccgtt ttctacaacc ttagtcacat caacaaggaa gagatggttt
    12721 tcagttccac ctgaaataat acggaaatca gggtcttgca agaagacatc tgccatagcc
    12781 ttgctgttct taattacatt ggcagcatat tccttgaagg ctggatccaa aacttctttg
    12841 aaggaaactg ccttagccgc cacaacatgc tctaaaggac cgccctgaat acctgggaaa
    12901 atagctgaat tgattttttt agcaagttct tcgtcattgg tcaaaatcaa accaccacga
    12961 ggtccacgaa gggttttgtg ggtcgttgtt gttgtgatat gagcgtatgg aactgggctt
    13021 ggatgaaggc cagccgcaac caaaccagcg atatgggcca tgtccaccat gagcttcgca
    13081 ccgacagcat ctgcgatttc acggaatttt gaaaaatcga taatttgaga atatgctgaa
    13141 gcaccagcta caatcagttt tggttttact tcttgggctt gtttcaagat agcatcaaag
    13201 tctaagagtt ctgtttcagg atccacacta taagaaacaa agttgtaggt ttgaccagag
    13261 aagctaacag gagccccatg agtcaaatga ccacctgatg ccaaatccat tcccataacc
    13321 gtatcacctg gctcaatcaa ggacatgtaa gccgcacagt tagcttggct tcctgaatgt
    13381 ggttggacat tggcaaattt agcaccgaaa atttcttttg cgcgttcaat agcaagagtc
    13441 tctacaacgt ctactacatc agttccacca taataacggc gtcctgggta accctcggca
    13501 tatttatttg tcaagataga cccttgagct gccataacag ccttggaaac tacgttttcc
    13561 gaagcaatta actcgatatt attttgttgg cgttcttctt ctttggcaat agcattccag
    13621 agatcagcat catatgcttt aaaatcatct ttgtcaaaaa tcataggtct tctcctttat
    13681 tgtgtgacta gtccattagt ttgattttac aataagaaaa tcaaactaac agatgcgaat
    13741 aaaccgtttc tgcattttat cacaagtata gccaactttt tcataaaatg catgagcacc
    13801 cagacgatga ttggcagaat ttaagcggat aaacccataa ccacatcttt ttgcttcttc
    13861 ttccaaccct tgtagtaaac ttttaccaat accttgacct tgcgcttgag gtgaaactgc
    13921 taaagctaag atattaaatc ctgctttgga atagagtgat tcgtaaactt cagcgtggac
    13981 atatccaagt aagacatgat tagctgcatc ctcatagcca agtaggaaat gatgggaatc
    14041 ctgagacagt ctagctagtt ggctagccgt ttcctctgga ctaaaagtat aacccaaagc
    14101 ctcttggttg atgtcacata tcgctttcac atctgtttct tgcaaatctc ttagcatttc
    14161 aagcctcctc aaaagaaatc tctggcaacc gagcaagaat atcttctcgt ttaattgcgc
    14221 cttggcgtaa gattttcacc ttgtctccag acaaatccac aatagttgaa tcctgtccag
    14281 ttagaaaagc atcgtcttcc agacccagaa cctcttggtc aaaatccttc agaatttgtt
    14341 caaaggttac accacttgcc tgacctgaga tattggcaga cggcccaatc aagggacctg
    14401 tctctcgaat taaatccagt gtgatagggt gactgggcat ccgaaatcca atagttgcaa
    14461 ggtcagaatt tacccaatag ggaactcggt cattggcttc gagaataatg gtcaagggac
    14521 ctggcaaaaa ggtctctaca agtttttgta gataagctgg ctgattctta gaaaagtgca
    14581 agatgtcctc gaaagaggcg atattgagat tgagtgcctt atctctagga cgacgtttga
    14641 gttggtaaac atggtcgaca gctttttcat ctaaggcctt agcaaaaaga ccgtaaaccg
    14701 tctctgtagg tagaacgaca gctccaccat tttccaactc ttgtctaatc ctgtccatca
    14761 tcaaccacaa ccatcctgtt ttgaccaaat tggtccttga gtgttcgaac tcttttttca
    14821 ggaagatgtt tcctaaaaag ttcgggaaca ctttgacctt gcttgtatcc aatttcaagg
    14881 taaatcttac caccatcttt gagatagtct tttgcatctt ccgcaattct acagtaaata
    14941 gctaggccat cctcgtctgc aaagagagct agatgaggct ccgaatgcaa aacattcaag
    15001 cctacctctg actcatcttc acgagagata tagggtggat tggatacaat tatatcatat
    15061 ttttcagaaa tttctgcaaa acaatcagat tttttaaaaa atatattaag attttgattg
    15121 ctagcattct ctgatgcaag ctctaaggcc tcttgagaaa catctgctgc tgtcactgac
    15181 caagctggtc tattttttgc tagagcgaga gcaatagctc cactacctgt tccaatatcc
    15241 agaactgaga gattcgtctc aagattttca gttagaataa gctccactaa ctcctctgtt
    15301 tctggacgag gaatcaaaac ccgttcatcc acctttaaat gcattccata aaaatctgcc
    15361 tgtccaatga tgtactgagc tggcttgtga gctgctagtt gctggtaaat atcttctaca
    15421 aattgttttt cttcctctgt tgtcacctcc tgctggaggg caaaaataaa gtctgtaaaa
    15481 gatagatttt tcagactacg atagacaaaa gagaggcttt ccgcttcctc tccttgtctt
    15541 atcaactctt cttcaaaatt tgaaaataat tgagctaatt tcattatttg tttaattctt
    15601 ctagtttttg tgtttggtca taaagcacca aggcatccac aacttcgtcc aatttaccag
    15661 acaaaatcgt atctagtttt tggagggtca agccgatacg gtggtctgtg acacggtttt
    15721 gtgggaagtt ataagttcgg atccgttctg aacggtcacc agtaccgatt gtcgacttac
    15781 gctcagcgtc ctgctcatct tgagcaatct gagcaaagtg gtcagcaaca cgggcacgga
    15841 tgattttcat ggccttctca cggtttttct gctgggtacg ttcttcttgc atctcaacct
    15901 tgatattggt tggcaagtga acgatacgca cggcagtcgc aaccttattg acgttctgac
    15961 caccagcacc tgatgcgtgg taaatatcaa cacgaagatc ttttggatcg atatcgtatt
    16021 ctacttcttc gacttcaggc atgacaagaa ctgtggcagt tgaggtatga acacgacctt
    16081 ggctttctgt cacggggaca cgctgcacac ggtgggcacc tgattcatac ttaagcttag
    16141 agtatacaga ctgacctgaa accatagcaa ccacttcttt aaaaccaccg acaccattca
    16201 tagaggcttc catgacttca aagcgccaac cttgggcttc cgcatacttt tggtacatag
    16261 ttagcaaatc tccagcgaaa agtgccgctt cgtctccacc agctgctcca cggatttcaa
    16321 ggatgatatt cttgtcatcg tttggatcct ttggaaggag caaaattttc agtttttctt
    16381 catattcttc tttttcagcc ttggcatctt tgagttcttg cttggccaat tcttccaagt
    16441 ccgcatctcc gcctgattcc ttaatcatct cttcggcatc gacgatattt tgaaggactt
    16501 gtttatactc acggtaggct attacggtgt cacgattgga agcttcttct tttgaaagct
    16561 ccataaaacg cttggtgtct gaaacgacat cagggtcact cagcaattct cctagttctt
    16621 cataacggtc ttctacagct tgtagttgat catagatgtt catttttctt cattctcctt
    16681 attgatttca ggggcaaaat aatgtttacg gcaaaccgag atataggttt cattaccacc
    16741 aatctggatc tgttctccat cgtaaacggg cagtccatcc tgtgttcgca acaccatggt
    16801 cgcctttttc ttgcaatact gacagatggt cttgatttcg tcaatcttgt ctgctaaaag
    16861 caagagatat ttggaacctt cgaacaattc attgcgaaag tcatttttca agccaaaagc
    16921 catgacgggt atgtctaact cgtccacaac acgagctagg tcataaacat ggtggcgttt
    16981 gagaaactgg gcttcatcga ccaaaacaca gtaaggtttt tctggtaggt ctcggatata
    17041 gccaaagata tccgttgttt cctcaatagc aagggcaggg cgtttcatgc caattcgact
    17101 cgacacatag ccaacaccgt cacgcgtatc cagagccgag gtcataatca caacaccttt
    17161 tccttgctcc tcgtagttat aggccacttt gagaatctca atcgttttac cagagttcat
    17221 ggtcccataa cgatagtaca actgtgccat gtttcttgct tcacgtccat ttctaaattt
    17281 ttgctacatt ctagtatatc ataattttct taagctttaa acggcaaaat gtggtaaaat
    17341 ggaagaaatc aaaactagtg gaggaagcta ttatgccatt tgtacgcatc gatttatttg
    17401 aaggacgcac gctcgagcaa aagaaagctc ttgctaagga agtaacggaa gcagttgtcc
    17461 gcaacactgg agcacctcaa tctgctgtcc atgtcatcat caacgacatg ccagaaggaa
    17521 cttacttccc acaaggggaa atgcgtacta aataagctag cttaagcaga attgcttagg
    17581 ctttttcaat ctccaagtag cacccattga agaaatagtc taaatttgtt acaatttgaa
    17641 aagagacttg gaaaattcca agaaaagagc aattaattaa aggaaacatt atgattacac
    17701 gtgaatttga taccatcgct gctatctcta ctccactagg tgaaggagct attggtattg
    17761 tccgcctgag cggaacagac agttttgcta ttgcgcaaaa gatttttaaa ggaaaagact
    17821 tgagtcaggt tgctagccac actctcaact acggtcacat tattgatcct ctgactggta
    17881 aagtcatgga cgaggttatg gttggggcta tgaagtctcc aaagaccttc actcgtgagg
    17941 atattatcga gattaacacc cacggtggta ttgcggtgac caatgagatt ctccagctag
    18001 ctatccgtga aggagctcgg ttggcagaac ctggtgaatt taccaaacgt gcctttttaa
    18061 acggtcgcgt agatttgaca caggccgagg cggtgatgga tatcatccga gccaagacag
    18121 acaaggccat gaacatcgcg gtcaaacaac ttgatggttc cctttctgat cttatcaata
    18181 atatccgtca agaaatcctt aacacacttg cccaagttga ggtcaatatc gactatcctg
    18241 agtatgacga tgttgaggaa gccactactg ctgttgtccg agagaagaca atggagtttg
    18301 agcaattact aaccaaactc cttaggacag cacgtcgtgg taaaatcctt cgtgaaggaa
    18361 tttcaacggc tatcattgga cgtcccaacg ttgggaaatc aagccttctc aacaacctct
    18421 tgcgtgagga caaggctatc gtaacagata tcgctgggac aacacgagat gtcatcgaag
    18481 agtacgtcaa catcaatggt gtacctctca aattgattga tacagccggt attcgtgaaa
    18541 cagatgacat agtggaacaa atcggtgttg agcgttcgaa aaaagccctc aaggaagccg
    18601 acttggttct actagtgcta aatgccagtg aaccactgac tgcgcaagac agacaacttc
    18661 ttgaaattag ccaagatacc aatcgcatta ttctacttaa taaaaccgac ctgccagaaa
    18721 cgattgaaac ttcgaaacta cctgaagacg ttatccgtat ttcagtcctt aaaaaccaaa
    18781 acatcgacaa gattgaagag cgaatcaaca acctcttctt tgaaaatgct ggcttggtcg
    18841 agcaagatgc tacttacttg tcaaacgccc gtcacatttc cctgattgaa aaagcagttg
    18901 aaagcctaca agccgttaat caaggtcttg agctggggat gccagttgat ttgcttcaag
    18961 ttgacttgac tcgtacttgg gaaatcctcg gagaaatcac tggggatgct gctccagatg
    19021 aactcatcac ccaactcttt agccaattct gtttaggaaa ataagaaaaa tccatgatcc
    19081 ttcattcggt catggatttt attgtcttta ttagtaatct ggtcttaaga cccctgttac
    19141 agttgcctta gttgcttcgt agtcgccatc tacgacaacc ttgataatgc gtttggcatc
    19201 ttcttctggt gctggaacaa gaggtagacg agtgggtcca gcttcaaatc ccatatagtt
    19261 aagaattgcc ttaactggag caggacttgg ataagagaag agagcattaa ccttaggaat
    19321 gaatttacgc tgaattgctg cggctttctt catatcgctt tctgcaatgg cagtaaacat
    19381 ctcgtgcatt tcatccccat ttgtatgaga ggcaacagaa ataaccccat ccgccccaag
    19441 gttcatggca tggaaagcat ctccatcctc acctgtataa atcaagaact cttcaggctt
    19501 gtgctcaatc aagtaagcca tattagccaa gctagtacat tctttgacac cgataatatt
    19561 tggatggtca gccaagcgaa gcatggtttc tggagtcaat tcgacaacta cacgccctgg
    19621 aatgttatag ataataattg gtaggtcaga agcatctgca atagccttaa agtgctgata
    19681 catcccttct tgagaaggtt tgttgtagta aggaacaata gcaagcccag ctgcgaaacc
    19741 accaaattcc gctacttctt tgacaaactc aatagagtca cgcgtatcat tagtacctac
    19801 acccgcaatc aaaggaacgc gtccattgac aaccttttgt acagccgcaa acaactccaa
    19861 ctcctcatcg tgggtcaaag ttggactctc agcagtcgtt cctgcgagaa gaattccatc
    19921 cgtatgatgg gccaataaat gctcaatcaa ggctggaata gcatcaaagt taatggaacc
    19981 atcctcatgg aagggggtaa taaaggctgt aatgatttta cattctttta aatcttgata
    20041 agacacgaaa acacctctct atttcaaaga aggagttcat ctgaactcct aaacgatatg
    20101 actattttaa ttcaaatttc aattcggctg ttggacgaac caatccacgt tcatgaagag
    20161 tttcagcaat ctgaactgag ttccaagcag cacctttgag aaggttatct gaaacaaccc
    20221 acatgtgaat tcctttttct gcatccaagt ctttacggat acgaccaaca aaggtatcac
    20281 gcgaaccaac tgcattgata gcttgaggat agatttgatg agctacatca tcttcaagaa
    20341 cagcacctgg gaaggctgcg atagctgctt ttacttcttc gattggagcc acttcttttg
    20401 tttcgatata aacagactca gagtgagctg acaagactgg aatacgcaca catgttgcag
    20461 atactgcaat gctatcatct tccataattt tcttagtttc cttggtcatc ttcatctctt
    20521 cgtacgtgta atcattatca gtgaaaacat caatttgtgg aagagcgtta aaggcgatag
    20581 gataatgttt cttgtcacca cctgaaggca aaatttccgc atgcaaatca catggtttca
    20641 caccatcatt caagacttca cgaagttcac gttgtgtctc aagaattgct cccataccag
    20701 cacctgaaac ggcttgataa gttgaaacaa tgatacggtc caagccccat ttttggcgaa
    20761 ccggctcaag agccaccatc atttggattg ttgaacaatt agggcaggca atgattccgt
    20821 tgtgagcatc aagtgcatga gcattgacct ctggaacaac caaaggaaca tctggatttt
    20881 gacggaaata agatgtatta tctactacta ccacgccagc ttttactgcg tatggtgcat
    20941 acttagctga tgtagaacta cctgctgaaa agagagcaat atcaactcct tcaaaagctg
    21001 tttcagtcgt ttcttcaatt gtaatatctt gatctttaaa tttcaatgac ttacctgctg
    21061 aacgtgcaga agcaaggtaa cggattttat cgattggaag tgttgattct tccaacattt
    21121 ttatcatctg agcaccgacg gcacctgtcg cgccgactac agcaactgta tatcccataa
    21181 ataacctctt tcggaatttt ctaaaaattt ctataataga agtattatac tactttttcc
    21241 atgaattgca aagctttttc atcatttttt ggaaaataaa aatcccagtc gctagactag
    21301 ggactaagag gcatcttcat gtgatgagca ggttcacaca actcatcaag gtccgctcct
    21361 gcgttatgac ctccttatgc tcaatagtag tccgaagact acctatcgac tcatcgcata
    21421 ttctatatta ctagagatac ttgattttgt caataagaaa ttttttcgat cgagtataac
    21481 aaaaaaactc tcgtattcaa gagtttccct atacatatca tgctaattgc cgggattgaa
    21541 ccggcgacct catccttacc atggatgcgc tctgccaact gagctaaatc agcttactta
    21601 agaagtatac tatattcaga aaaacttgtc aagtttcctt agaaattttc cataggaaaa
    21661 agccaggtaa gaactaccta gcttatcttc ttctatttac ttaaatcaat tcgacgacca
    21721 attttaccaa taataatcgc tccaataatc aatgaagcaa attcaccgat tcctgttgta
    21781 aaccaagtaa ggaagaatgg taattctgct acaatattta actctgcagc gatagtaaac
    21841 atggaaattg aaaagaggat tgaaaagaag aaatgatctt ttcgaatcaa tccgttaaag
    21901 aggtaatcct tgctatattt tgcaaaaagc caaacaccta aactgaggaa tactaaggtt
    21961 gaaccaccac caacaaagac atctagcagt ccgaagctaa agaaattagc aatcatacaa
    22021 ccgatggtaa ctccgatgat gtacttagga ttgtaaaaag ccataaagtt catcatttct
    22081 gaaatacgaa actgataagc accatagctg atggcattta ggggcggtgt gacagtcaaa
    22141 acgacgtaga tagcagcgac gattgcaata tctgcaacat cacgaatagt taattttttc
    22201 atcttttctc ctttggcggt tgcccgcgtg taatatgctt ggtgaaagaa gctaagcacc
    22261 aagggttgct ttattcaacc ctactagtat agcacaatag cctgctttat gctatactta
    22321 acacatgaaa attcctattc aaaaattttt taacaatgaa atcttagcct atctcttttt
    22381 tggtatagtg actactcttg ttgctatact attacgatta gtcatttatc agctaagtca
    22441 tcaagaactc cttgctacgg ccttggcaaa tattatcggt attttatttg cctttatcac
    22501 aaatgataca attgtattta agcaaaaaaa gaaaaataga cttatccgtt tagtgaaatt
    22561 ttctcttgct cgcctttcta cttttctgtt agacttgttt ttaacttttc tctttgtaac
    22621 ccaatttcct cacatcatag ggcaattcgt aactgaaaac attgataaga taaatgctat
    22681 tgaaacaata attgcacaaa tattcattat tatcatcaat tatatcttga gcaaggtcta
    22741 tatttttaga aaataatgct ccatttatga gccttttaat tgccttctat aatgatttag
    22801 gatacaatga aacacgaaac attgaaagga aaacagtatg ttaaaaaatc taaaatcgtt
    22861 cttgcttcga ggaaatgtta ttgaccttgc tgtcggtgtt gtaattgcct ctgcttttgg
    22921 tgctatcgtt acttcacttg taaacgacat tatcactcct cttattttaa atcccgcttt
    22981 gaaagctgct aaagttgaac gtatcgctca actttcttgg catggagtcg gctatggtaa
    23041 cttcttaagt gctattatca attttatctt tgtgggtacc gccctcttct ttattatcaa
    23101 gggcattgaa aaagcacaga aactgactgg cataaaggaa gaaaaaactg ccgaaaaaaa
    23161 accaaccgaa ttggaagtcc ttcaagaaat aaaagctctc cttgagaaaa aataatcgat
    23221 taaaaggatc tagcgcaaag ctaaatcctt ttttctttac tctttgggta acttgcctgc
    23281 tttttcaagc atttttttga taagataagg catcttaaca ttctcccgcc cttttttctc
    23341 aatcagtttt ttcacaaatt ctggcatttg taaatcttga gattcggcta agatattttt
    23401 agtctcatct gaaggcacta actcatcaaa ggtttcttct tctgggagaa attccttaaa
    23461 ctcttgattt tcattgactc taactgtatt gaccaaggca tcaatcaaac gagaatctgt
    23521 atttggcatt ggtggacgat ggtagtttac cccaaattcc tgacataaat cataacattc
    23581 cacatcattg tcaaacaaga cctcaatatg ctcactgatg aaactaatag gtacaaaaat
    23641 ataatggtct ggatgttctg tctgttctct gagatactcc aaaacatctg gtttaatcca
    23701 tggaatacca atatcactct cactctgcca agtgttggta tattgttctg aactcaagcc
    23761 cagtttttca gcgactagct tgctattttc gaaaatttgg tctatataag gatctccaaa
    23821 gtccaaggca aaaataggca cactatgggc tgaaaagatg actttaaagc tgtcttgctt
    23881 tacttcttct tttaaaatct tagcaatttc atctgcccaa tagtttaaga gcgcttcttc
    23941 ctgataccag tccttaatga ccaaaaattg aatttgtttg ctttctaaaa atttttcata
    24001 gcccatgaca gagtagaaag aataatgggg ctccaaaatc aagcaaatac actgttcaat
    24061 tccgtctgct tccatttgac cgatcacatc tggaataaag ggactggaaa acttattagc
    24121 aaagtagaca ctgtattcat tccccagcct agcttctacc aaggcaacct cttgacgggt
    24181 aattttttgc agaggcgtgc ctccaattcg tacataatta tcatagagag tctgaatctc
    24241 gtggtcttgg ggtctcatcc cacgacgaat atttgtgaaa aaatcagcta caccttcaaa
    24301 ggtaatctct tctggtgaac cgaaggtcat cattaaaatt gcttttttca tgactgcttc
    24361 cttgtgatgt ttttatcaag tttattttat cattaattca tcgataagta aacgctagca
    24421 tagggacatt ctcaatcttc tgtcttttgt ttttctcttc tttctatgat acaatggaaa
    24481 aaataaattc aaaaggagtt tttttatgac ttatccaaat ctcttggacc acttcttaac
    24541 ctacgttaag gtcaacacgc gctctgatga acactctact actactccaa gtacacagag
    24601 tcaggttgac ttcgcaacaa atgtcctaat tcctgaaatg aaacgtgttg gactgcaaaa
    24661 tgtttactat ctaccgaatg gttttgctat tggaaccttg ccagccaacg atccgtcttt
    24721 aacacgtaag attggtttta tatcgcacat ggatactgct gattttaatg ctgaaggagt
    24781 caatccacag gtaattgaaa actacgatgg tggtgtgatt gaactaggga attctggttt
    24841 caaactcgat ccagctgact tcaagagtct tgaaaaatat ccaggacaaa cgctcatcac
    24901 aacagatgga acaaccttgc taggtgctga tgacaagtca ggaattgctg aaattatgac
    24961 agccattgaa tatctaactg ctcatcctga aattaagcac tgtgagattc gtgttggttt
    25021 tggtccagat gaagaaatcg gtgttggtgc caataaattt gatgcagaag attttgatgt
    25081 ggattttgcc tacactgttg acggtggtcc actaggtgaa cttcagtacg agactttctc
    25141 agccgctggt gctgaattgc atttccaagg ccgtaatgtc caccctggta ctgccaaagg
    25201 gcagatggtc aatgcccttc agctagcaat tgattttcat aatcaacttc cagaaaatga
    25261 ccgacctgag ttaactgaag gttaccaagg tttttaccat ctaatggatg tgacaggtag
    25321 tgttgaggag gtgcgtgcaa gctacatcat tcgtgatttt gaaaaagatg cctttgaagc
    25381 gcgtaaagca tccatgcaat ctatcgctga taagatgaat gaagaacttg ggagcgaccg
    25441 tgtcactctc aacttgacag accagtacta caatatgaaa gaagtcattg aaaaagatat
    25501 gactccaatt accattgcta aagccgttat ggaagatcta ggtatcacgc ctattatcga
    25561 accaatccgg ggtggaacag acggctctaa gatttccttt atgggaatcc caactccgaa
    25621 tatctttgca ggtggcgaaa atatgcacgg acgttttgaa tacgttagcc ttcagactat
    25681 ggaacgtgca gttgatacca tcattggcat cgtagcttat aaaggctaaa aagacgaggt
    25741 agctcagcta cttcgccttt ctttttattc tactggtttt tcttgatttc cagtagttgt
    25801 agaagattct gttgtttcat tttctgaagt tgattcagca ggtttagaat ctcttgtatt
    25861 gcttggtttg ttttcgtcgc tagcagtttc aatgttagat tctgcagttg cgtttggttg
    25921 gttctcagca ctggtgttat cactatttgc ttcagcattt cttgctggac ttgtttcttc
    25981 acttgcgcta gcttttgact ggatttgatg attcaaaact agaatagctt ttgtcgattc
    26041 aagtaaagct gttttgtctt tactcttagc agaaagttga tctaataatg catccacctt
    26101 atcaaagtcc gcatcagatc cattattact ttctaaataa gagtgaagcg acatgagaat
    26161 atcgtagagt ttttgataga gtacaagtgt ctgagaatct tgctcagcat tttccttttc
    26221 ttgttgaagg gcgctagcga tacgagtcaa aacatctttt acctgactgt ttacttcatc
    26281 caagtctgca tcagccttgt ttgtggcagc ttttagattt tctacttctt ctgccaagga
    26341 ttgtctgatt ccttcttcat ggatttgttc caagagttga tttgccttgt tcaaaaaact
    26401 ttctacttct tccttgctat ctgtcgcaga ttattggttg ctatctacca tgtactccta
    26461 aaacaggaga gttataatcc aagattacaa ggccttacag aaataagaaa tccagataag
    26521 acaatgttcg tccaagacgc tattcgcttc gcacagcagc acggattcaa tatactttaa
    26581 ttttaaagtt taggtgtcaa gacctctttt tagtgtgccc aaaatttaga gaagtaatca
    26641 atcaactaac ttttattttt ttcaaacttt cagtaaactg acctaaagct aactcaatct
    26701 gtctttgttc gataggcttg tctttgtaga tgcttctgct atcagctaga agttgatcta
    26761 cttttgccaa gactgccttc tcatcaaaag ttccaggttg atagttggat tgcagggatg
    26821 gaatcttgtt tttcaaagcc gcttcatatc ccttagtttg aaccttgatg tagtgattgt
    26881 ggtcgccacg aggaatcaca aaaccttctg aatcttcact tataattcga ttggcatcaa
    26941 aaccatgacc atcttcttcc tcatggtgga catgtagtga cggattactt aatacagaac
    27001 tagaagaact tcctaccttt tccgtgttag agtgtgatgg gggattgtta agagatgact
    27061 taggaatata gtgatagtga ccccatgtct tactatataa gcatcacctg tatctctgac
    27121 aatatcatta gggttaaaga cataaccatc atctgctgca gaaacaccat tattcggtgt
    27181 caccgacaaa gattgactga gagctgtagt attctctgat aattatactt ttgcagctgc
    27241 taattcacct gccgacaagt cactctcagg aatgaaatga tagtgaccac catgtggtac
    27301 tatagtagat tgaaatagaa tatgagcaaa ttgataaggg gattttaaag taatttctaa
    27361 caatgattta gaaactatga tgtgctattc taaattcaac tcactatata taaccatcat
    27421 cggtagtata acgtccctgt aattttgcta cagatacttc tgcactagct cctttatcgt
    27481 ctttaccatg ttcttgtttt tggcgattga tttcatcttt tattcgtaca ttttctgcat
    27541 gagcttgatc tttaaggtaa acataatact ttccatctac cttaataata tatcctccct
    27601 taacctaact gacgatatct tgatctttcg gctgatagtt gggggctttc attaatagct
    27661 cttcactaaa gagcgcatac aaaggaactt taccattata gcagtgataa tgatcgccat
    27721 gagaagttac ataaccttga tctgtaatct taataacaat ttgttttgct tgaattcctt
    27781 ctttttgact aacctagtct ggagtcaaat tttcagtctt cttagtgtct ttattactgt
    27841 ttacatatga aacacgattt ttatctgtat tggcctgtta gctatgttgg ttcagagcat
    27901 aaacacacag acttaaggaa aggataacaa cagatccagc tgttatatat ttctttttaa
    27961 atttcataat tacctcattt ctataattat ttatatgatg tcttcattat ta
//
LOCUS       DBEROU010000028        28005 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.028, whole genome shotgun sequence.
ACCESSION   DBEROU010000028 DBEROU010000000
VERSION     DBEROU010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 28005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28005
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.028"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(43..1191)
                     /locus_tag="ACL8CL_001297"
     CDS             complement(43..1191)
                     /locus_tag="ACL8CL_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS1330540.1"
                     /translation="MKYNKTKYPNIYYYETAKGKRYYVRRSFFFRGKKREKSKSGFTT
                     LPQARAALVELEQQIQEQELGINTNLTLDQYWDIYSEKRLSTGRWNDTSYYLNDNLYK
                     NHIKAKFGSILLKNLDRNEYELFIAEKLQNHTRYTVQTLNSSFMALLNDAVKNGNLLS
                     NRLKGVFIGQSDIPAANKKVTLKEFKTWIAKAEEIMPKQFYALTYLTIFGLRRGEVFG
                     LRPMDITQNDSGRAILHLRDSRSNQTLKGKGGLKTKDSERYVCLDDIGTDLIYYLIAE
                     ASKIKRKLGIIKEQHKDYITINEKGGLINPNQLNRNFNLVNEATGLHVTPHMMRHFFT
                     TQSIIAGVPLEQLSQALGHTKVYMTDRYNQVEDELAEATTDLFLSHIR"
     gene            complement(1365..2177)
                     /locus_tag="ACL8CL_001298"
     CDS             complement(1365..2177)
                     /locus_tag="ACL8CL_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HGS1330541.1"
                     /translation="MQKTVEKILFRVAGVTKYKKAVKEACNMIAEDNGIPEYAKYYSN
                     LSTKELREELEEYGLKVFKYQDLDFFNIELVPEVDNRYDPNAIKVLIFDNHVGYVPAT
                     IAKTIRKYFDNKKYNFVIEGEIKGGPYKEWDEYEEKVVTNNDLDVGFEIYLTIVDSSQ
                     KEVIQSESSEIIDDNISNKEVTETEAIETKTTETEHIEQNIVSDSVADIVNEINLSET
                     SPKKKLPANKIIFSALYLFLVFFGVVGIPIAPFLAVPLTAWSLYKLYKLFRK"
     gene            complement(2215..2595)
                     /locus_tag="ACL8CL_001299"
     CDS             complement(2215..2595)
                     /locus_tag="ACL8CL_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGS1330542.1"
                     /translation="MTLAKLCEEYQVELCLFDGSNWHSSGFYNPDTNVLAIDHNLAPE
                     QQIQVALHELGHKDHTRSEYQNARLRCENEADRNMIHHLVKDALESLDDPTEFDYLKF
                     MSYYNLKTMTNEVMVKEEYLALVN"
     gene            complement(2608..2871)
                     /locus_tag="ACL8CL_001300"
     CDS             complement(2608..2871)
                     /locus_tag="ACL8CL_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HGS1330543.1"
                     /translation="MYRLDIDKKALKQLKKLDTPTRKQILSWLAKNIENTTNPRQHGK
                     ALKANLAGYWRYRVENYRIICDIQDDKLVVLAVEIAHRRDVYK"
     gene            complement(2871..3104)
                     /locus_tag="ACL8CL_001301"
     CDS             complement(2871..3104)
                     /locus_tag="ACL8CL_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330544.1"
                     /translation="MTITINFTEKNSYITDYLTKHGIDTTTMDFDDFMVLMEDIEDAR
                     AADQAYMEYLADPATYTMDEVLDELGLSREDIA"
     gene            complement(3104..3472)
                     /locus_tag="ACL8CL_001302"
     CDS             complement(3104..3472)
                     /locus_tag="ACL8CL_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1330545.1"
                     /translation="MFVAFDKIKELADKQGISINILEEKLGYGTNTLYRLKRSNPSSK
                     VLKEIADYFNVSADYLLGRTDNPAIAGSDEFAQVNGQIIDLRKAAANTMLFDGKPLNE
                     DDIDFITSVLSAHFKSKGER"
     gene            4161..4364
                     /locus_tag="ACL8CL_001303"
     CDS             4161..4364
                     /locus_tag="ACL8CL_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGS1330546.1"
                     /translation="MSKELKIIKAKIKTRLIELDMTQAELAKQVSVTSSVISELLKYG
                     KGSDYVKEKVVDILGIENPWENS"
     gene            4381..4578
                     /locus_tag="ACL8CL_001304"
     CDS             4381..4578
                     /locus_tag="ACL8CL_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330547.1"
                     /translation="MNNAAQKVTRIDKDAWEIATELANEYGVSICHIISESVRYCAEN
                     AEFKEMDVVVKRLVVGSKVLE"
     gene            4589..4750
                     /locus_tag="ACL8CL_001305"
     CDS             4589..4750
                     /locus_tag="ACL8CL_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330548.1"
                     /translation="MNELVWFYFVVIINLIIGFATYYASKRDREKRINGFKKIQDDEL
                     ERVRNKFNL"
     gene            complement(4745..5170)
                     /locus_tag="ACL8CL_001306"
     CDS             complement(4745..5170)
                     /locus_tag="ACL8CL_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000456432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330549.1"
                     /translation="MEYQALIQPVISIILAIISGLWSYVASKANNKAEIEKQAKEHSH
                     TVEKLEREFHHQIDTLKQQHAFELEKIKQAHELRLQELEKVSQLDTETDKSMKMNDLT
                     YKVFTGEVDFDKALKIANKASNSKQNLNKRFIQKTSKKS"
     gene            5224..5934
                     /locus_tag="ACL8CL_001307"
     CDS             5224..5934
                     /locus_tag="ACL8CL_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HGS1330550.1"
                     /translation="MNEIFNFHGQEVRTLTIDNEPWFVGKDVADILGYSKARNAIALH
                     VDEEDALKQGIPTSGGTQDMLIINESGLYSLILSSKLLQAKEFKRWVTSEVLPAIRKQ
                     GGFIREDLDEDAFIALFTGQKKLREQQATMLEDIDYLKSEQPIHPSYAQSLLKKRKAR
                     VVACLGGIDSPAYADKVFSQSVFRQAEIDFKDHFNISRYDLLPKKHADAALAYWMTWE
                     PSTNTKMKIMKLNSFDDV"
     gene            5948..6205
                     /locus_tag="ACL8CL_001308"
     CDS             5948..6205
                     /locus_tag="ACL8CL_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HGS1330551.1"
                     /translation="MDNVLLSLSEWIKSIIKDTITRLVEIEKDSDHYPELMDVNTTCE
                     FLGIKYATFSDNYRYLKGFPKELPGKKWSKRAIKEWLSNQI"
     gene            6291..6611
                     /locus_tag="ACL8CL_001309"
     CDS             6291..6611
                     /locus_tag="ACL8CL_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330552.1"
                     /translation="MFEPPILDQLMGVGALLLGFAGACRHIKLQEQRKEEERREEQEF
                     ASMIIQVRNHAYERGREDKWQEIRKNIRREFKGFTYDNEPPVGLRPEPLALPEPKQSA
                     IRLL"
     gene            6627..6920
                     /locus_tag="ACL8CL_001310"
     CDS             6627..6920
                     /locus_tag="ACL8CL_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330553.1"
                     /translation="MEELIEWLETKEKLNREMISDEETSDFNLHLENDNDTMFMIKEY
                     LNDYNKLAKDYRILSSENRLLKLEKMELEGRYIYEDMRMKYRANRRKWGARYV"
     gene            6913..7713
                     /locus_tag="ACL8CL_001311"
     CDS             6913..7713
                     /locus_tag="ACL8CL_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HGS1330554.1"
                     /translation="MSEIKWIKITTDIFDDEKICLIDALPDPDAILVIWFKILTLAGK
                     HNSNGLLMMTDKVHYTDEMLATIFRRPLNTVRMAIGVFEQFGMIEIIDGIISLPNWEK
                     HQNVDGMEKIKEQTRNRVAKYRKKQKNLALGNVTGNVTVTDGNALEEDKDKNRLDKDK
                     NKKRITTTSSGSEENILELFQSEFRRLLSGFEIEEINHLLNENDVDLVKEALKTAINS
                     GKPNIKYIGGILRNWQMNNVTTVEQVRQSEKKNKDKKEEQEAKDEWGY"
     gene            7853..8623
                     /locus_tag="ACL8CL_001312"
     CDS             7853..8623
                     /locus_tag="ACL8CL_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS1330555.1"
                     /translation="MVDNVFEEIALSYRRNTEQQEEFCEKHNIPLIKILRTESVVCRM
                     CESERIHEENQERVNELANAENERERKYYLEKFSLYDEVLKNATLDNFETPTEKEAEK
                     LAFAKRICREWSEGARNNIVLQGEAGTGKSHLAFAVVKALSEYTKEIAIFINVTDFLM
                     KIKADFSQEEFLVNKIASAKFLVLDDLGMEKDSEWSFTILYNILNKRSNTIITTNLIS
                     ADIQKRYGRPFMSRLMKGVDKDHLMVFNDLTNKRKQYF"
     gene            8638..8832
                     /locus_tag="ACL8CL_001313"
     CDS             8638..8832
                     /locus_tag="ACL8CL_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330556.1"
                     /translation="MFILKHGTREDKPFLRSAIISVTGLDISCSEEKKAMRFISRAAA
                     LQVSKALRGSFGNFYPVEVE"
     gene            8832..9059
                     /locus_tag="ACL8CL_001314"
     CDS             8832..9059
                     /locus_tag="ACL8CL_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330557.1"
                     /translation="MLELYFVYNGHCKFYLGTFDNVDDLIEQMEDHQWAFSAITHPRF
                     QKHIGQRTTRFDYGSKDCYYLATFSGGEKND"
     gene            9232..9354
                     /locus_tag="ACL8CL_001315"
     CDS             9232..9354
                     /locus_tag="ACL8CL_001315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330558.1"
                     /translation="MKEQYGQGRPDYIEALNDLDGLVEVSYREAIERFLEDEVR"
     gene            9525..9851
                     /locus_tag="ACL8CL_001316"
     CDS             9525..9851
                     /locus_tag="ACL8CL_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330559.1"
                     /translation="MEELNQKVNAVYNWTVEDGKPKPPKQDLPQAVKDRADYFWEMAE
                     DGMTFMGAMECIFADEKPTDYELGATKGWLPKSKEFDDWVGYSPSMTQVVIAVYLIYG
                     GSEDET"
     gene            9841..10314
                     /locus_tag="ACL8CL_001317"
     CDS             9841..10314
                     /locus_tag="ACL8CL_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HGS1330560.1"
                     /translation="MKLNELIKKYKKLEGVWNAEGAELARQIFLQDLEQLDKQQPVKV
                     KKFVADFIAEQKKLGHTLSYSIDASMSDIVAEWYWDNSELFALAWIFGCEVEEEKRYT
                     VVTKATKQPLYYNAMDKKLFFSMGGLATKFTRKQLKEADFGWVFDCPGIEIEEVE"
     gene            10314..10601
                     /locus_tag="ACL8CL_001318"
     CDS             10314..10601
                     /locus_tag="ACL8CL_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000194861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330561.1"
                     /translation="MTQTLEEGMKNQSKCIKVPREIRPFDIGYRIVNKYGQALALRNG
                     ASIFDLPFLAEKAIEKEFGKNDPDFDIGKHSVEEVAIVNLSKFHSYFEEVE"
     gene            10601..10774
                     /locus_tag="ACL8CL_001319"
     CDS             10601..10774
                     /locus_tag="ACL8CL_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330562.1"
                     /translation="MEDEQNILEIQLILGKQVLEIVLDLLKDDSKTGVVLPLNINDRE
                     FTITVEKEVTDRD"
     gene            10837..11969
                     /locus_tag="ACL8CL_001320"
                     /pseudo
     CDS             10837..11969
                     /locus_tag="ACL8CL_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333937.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            11966..12349
                     /locus_tag="ACL8CL_001321"
     CDS             11966..12349
                     /locus_tag="ACL8CL_001321"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330563.1"
                     /translation="MNTIEKVKQWFIDRDLENGRVVGLMVLSSKRRIYKMKRLGIIIG
                     AVFVIVVSPFVVQYGWNEIITTIVPVGKITVWQALGMDALLSFIWPVLSSKKESEEDY
                     SYAVKSSISKIITCAFLIWLASLFI"
     gene            12361..12771
                     /locus_tag="ACL8CL_001322"
     CDS             12361..12771
                     /locus_tag="ACL8CL_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HGS1330564.1"
                     /translation="MIPKFRGLSIDEKSKGEWQYGHLIEDRGRAFIINEVVEANEQYI
                     TIGSWCPVNPATLGQSTGLRDKNGKEVFIGDIVKCTRGCLHEVYLEKEYGGTYIGGMP
                     AVYLKGFGDGYAWTEYEEIIGNVYENPELLEVNE"
     gene            12908..13309
                     /locus_tag="ACL8CL_001323"
     CDS             12908..13309
                     /locus_tag="ACL8CL_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HGS1330565.1"
                     /translation="MKKLSDEDLKTLDRELFKFQNIQRTIDLRRLELETRNPDAQSGP
                     SVGISKPTETIAVRIADDPTLKFLEGFKAIINKLLINLVDEDKEIFNLRWRYPQLRWE
                     EIAEQKFMSKATIYRRRRIILEQYAILKGEL"
     gene            13500..14042
                     /locus_tag="ACL8CL_001324"
     CDS             13500..14042
                     /locus_tag="ACL8CL_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000397549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HGS1330566.1"
                     /translation="MEFVSPIKDNDDIQAMKDYLREWNEMYYMLFITGLNTGLRVGDI
                     LTLKVKDVQGWHIKLRERKTGKQITRRMTKELKKEMRRYVEGKPFHHFLFKSRQGQNK
                     AITRERAYQIIHEAAEELGIDNVGTHTMRKTFGYKYYNKTKDVGTLQKMFNHSSPAIT
                     LRYIGIEQAELDDALRNFVI"
     gene            14420..14740
                     /locus_tag="ACL8CL_001325"
     CDS             14420..14740
                     /locus_tag="ACL8CL_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HGS1330567.1"
                     /translation="MYNKPIRPSLKSKKWEKFRDRIMRKHDYLCQESLRYGISVQAEM
                     VHHIFPVSEYPELEFVEWNCLPLTNKKHNTFHDRVNDRVINQGLYWQKKRKKEFLNFF
                     KNEK"
     gene            14877..15269
                     /locus_tag="ACL8CL_001326"
     CDS             14877..15269
                     /locus_tag="ACL8CL_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HGS1330568.1"
                     /translation="MAKPITAKSIKSKVVKQMKDLGTYRKEFEMIIDIFAGMLYQYQK
                     LAQDYADMGYPVTDTYVNKAGAENERKVPILTAMEILRKDILSYSNQLMMNPKSLGEV
                     VEQEGESVLTEVLKFKNEIKKKRVTGNG"
     gene            15262..16992
                     /locus_tag="ACL8CL_001327"
     CDS             15262..16992
                     /locus_tag="ACL8CL_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HGS1330569.1"
                     /translation="MGNLDKAKEYAQHVLTHREEHCEENILAAERFFRDLENPIFEMD
                     EDMVDFVIHFIENVIVHQQGDDMFAVSIRNKPLLLQPWQHFVVVNLFGFYYKGTNERR
                     FKEALIMLARKNGKTSFTAAIALAYQILDTDSGSKCYIVANSVKQAMEAFGFLRFNVE
                     RWNDKNIRIKDNNQEHSITANFGDEGSFFIQALANDESRLDSLNGNVIILDEAHTMRN
                     SKKHGLMKKTMSAYRNSMLFVISTAGDIPTGFLANRLKYCQKVLKQLVTDDSFFIFIC
                     KANQSADGDVVDYLDENILKMANPSWEVTVSLKALKEEAEQAMNDPQTRNEFFNKTLN
                     IFTNSMNAYFNPDEFIASDSCYDWSLEELARLPIRWYGGADLSRLHDLTAAALYGVYN
                     DGEKDVDICITHAFFPRINAQKKANDDGIPLFGWQSDGWLTMSNTPTVLYDDIVKWFI
                     SMRERGFKIQAVGMDRKFGREFLAKMKKAKFKMIDQPQLFYLKSEGFRRIEFKVKNKE
                     FYYLHSDAYEYCVSNVRAIEKVDDAVQYEKLDGDGGTARIDLFDASVFACIQALANLG
                     KGGDVMRFFD"
     gene            17000..17218
                     /locus_tag="ACL8CL_001328"
     CDS             17000..17218
                     /locus_tag="ACL8CL_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330570.1"
                     /translation="MNEIVLSEHEINLLINKGRVKVILNGEVVTIRQRHMKNLMAETV
                     KWEKQVIDVSQNIVRNKHFDSLFQNTFR"
     gene            17236..18438
                     /locus_tag="ACL8CL_001329"
     CDS             17236..18438
                     /locus_tag="ACL8CL_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HGS1330571.1"
                     /translation="MGIFEKFWKRNKPSKSINMLSHSDLGLSNLMDSYVPLARNPDVV
                     TAVNKIADLVSNMTIHLMENTDKGDIRIRDGLARKIDINPCEHMTRKSWIFKIVRDLL
                     LYGDGNSVLHVEYEPVTDYISNLRPFPMREVSFQTDKDSYVISFRGEEYSPDEVVHFV
                     INPDPDIPYIGTGFRVTLTDVVQSLNMATKTKKSFMNGKNIPSLIVKVDSSSAELDSE
                     QGRERIAEKYLSTSRVGAPWIVPEALLDIQQVKPLSLTDIALNESVELDKRTVAGLLG
                     VPAFILGVGEFNKTEYNNFVNTTVMSIATTITQTLTRDLLLSSNRYFKLNPRSLFSYN
                     ITELSAVAQQMANSAAMRRNEWRDWLGMAPDPEMEELIVLENFLPQEKLGDQNKLKGG
                     EEENAKAK"
     gene            18422..18997
                     /locus_tag="ACL8CL_001330"
     CDS             18422..18997
                     /locus_tag="ACL8CL_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HGS1330572.1"
                     /translation="MQKRNSYRATQFQTREEESGDLILSGYFIKFDEETELWPGYCEV
                     IKRAGVEKAVTDADIRALFNHDDSLVLGRTGNGTLTLGVDDVGLFGDIIINKDDPQAV
                     GAYARVKRGDVIGCSFGFIPVKIETEEREDGSYLDTVLELEIFEVSPCTFPAYPQTEI
                     AARQKDFESQKRANREALDKRKKEIKEKFKL"
     gene            18994..20160
                     /locus_tag="ACL8CL_001331"
     CDS             18994..20160
                     /locus_tag="ACL8CL_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGS1330573.1"
                     /translation="MNKALIFGARMRAKATKVVELEETIEELNKRSVVELEKLDRAKN
                     DEEVLAVEKTVDGLQREIEEKEAEKVQLENEIDELDKQIKEQNRKAPTPGKMEERGGK
                     TLGQREAFNHYLRTKEARADGFKSAEGEAIIPVELMTPKEAKQDKTDLTSLVNIVNVK
                     NASGKWSVVKLTDQAMNTVEELEENPELAKPTFTKVNYEIKTRRGHLPVSQEFIDDAD
                     YDVMGLVAKQTKNQERITKNKEIAKVLKTATSKSAAGLDGLKDILNVELKTYYNATIV
                     CTQSMFAALDKIKDKDGRYMLQTDITSPTGYKFAGRVIVVYPDDIIGESKGDLKAFIG
                     DVGEFATLFDRAQTTVKWQDDKIYGQYLGTANRFDVKKVDEAAGFYVTYTDAVG"
     gene            20172..20462
                     /locus_tag="ACL8CL_001332"
     CDS             20172..20462
                     /locus_tag="ACL8CL_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330574.1"
                     /translation="MSYKVIRPFKDLADPENHDYAVGDIFPREGYEPTDSFTNGLLTG
                     ANTAGSIFLEVLGDDEPKKPAPETKEVKEEPAVEQEETVEETAEESAEEVEE"
     gene            20465..20746
                     /locus_tag="ACL8CL_001333"
     CDS             20465..20746
                     /locus_tag="ACL8CL_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000371962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HGS1330575.1"
                     /translation="MDQGQLLELLKLKLGISTDLRDKPLKKIISSVITELTDNLGIEL
                     VGERADHEMFIVDYAAYRYEGGVDMPRHLQWRLHNLQIASKKEVKNVES"
     gene            20733..21032
                     /locus_tag="ACL8CL_001334"
     CDS             20733..21032
                     /locus_tag="ACL8CL_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HGS1330576.1"
                     /translation="MWNHEITLISKKVTGKDKLLQPISEDVEVTLLCRKKRVTRSEFY
                     QANQVGLKPSLVVEIRNFEYENQELAKFEGKQYHILKTYPIDSEILELTLSEVLK"
     gene            21029..21376
                     /locus_tag="ACL8CL_001335"
     CDS             21029..21376
                     /locus_tag="ACL8CL_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HGS1330577.1"
                     /translation="MSNDLADLIAKELAAYSDEVTEEVDKIAEQVADETVDELKETSP
                     KRYGKYRRSWKKKKLANGSFVVFNAVASLTHILENGHLSRNGGRVAGIVHIKPAEEKA
                     IQNFEKRIKEIGK"
     gene            21373..21696
                     /locus_tag="ACL8CL_001336"
     CDS             21373..21696
                     /locus_tag="ACL8CL_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330578.1"
                     /translation="MKLSDFAAILEQANLPVTYRAFKIGNAPDLPYLVYYESSPVINS
                     ADNTVNHQIKSVTVELAFESKDEDLEERLEELWANHKLFFEVQEETFIETERLYVKSY
                     TVYLY"
     gene            21708..22286
                     /locus_tag="ACL8CL_001337"
     CDS             21708..22286
                     /locus_tag="ACL8CL_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HGS1330579.1"
                     /translation="MTQENKVTFGLENVHIAPIKTLAADGVITYGDVFRFPGAIELIL
                     DTKGETTPIKADNKDYHFMNSNEGYEGKLKIPHIIDEFATKILGEIKDPQTGVMTEKA
                     DASLTEFAMMFQFEGDKNKTRYVMYYCFASRPSLGSKTKNGTSTNERELSFKASPRPL
                     DTVVKRSITSADDKDAYDNWFKKVYEPTAVAA"
     gene            22298..22717
                     /locus_tag="ACL8CL_001338"
     CDS             22298..22717
                     /locus_tag="ACL8CL_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330580.1"
                     /translation="MRKIVLVGDQEYELGTNGYTPIAYKQQFGKDYFQDLFSMLKNQS
                     FMNELNKLETDKELTATNIDISMLSDFDMTFFNRLFWTFAKSANPHIKPYEQFFMEME
                     IFPIQEVGPVLMEMLNASMTTKKHQMSQNQLAKKSSQ"
     gene            22995..25754
                     /locus_tag="ACL8CL_001339"
     CDS             22995..25754
                     /locus_tag="ACL8CL_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000918321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HGS1330581.1"
                     /translation="MAGNIKGIKIEIDGDTQPLQKALKNVNKAATDASQELRQIDKAL
                     KFDTGNVTLLTQKQEVLQKQVSTTKEKLETLRQAQSQVEQQFKNGDIGADQYRAFQRE
                     VEVTQNVLKGYEGKLANVNQALNENGSATQNNKNQLKELQNEQSQLASEMSKVTSSFK
                     LQESALGSNASEAERNALAQKKIGAQSEIVSKQISNLEQQLEITKKEFGENSTQANRM
                     EAELNQAKTAFNHLNDEMKGTKSAADSTQESLSEISRNLRAELLQQFSEKLSAISEKL
                     VEVGKEALEAAAQMQASNAQFTTVFGDMETQAREALNAIGQEMDIVPERLQGSFTQMA
                     SFAKTSGLDTAEALDLTSRATRAAADGAAFYDKSIESVTESLQSFLKGNFANDAALGI
                     SATETTRNAAANKLYGKSFKDLSEAQKQLTLLQMVEDGNKLSGALGQAARESDGLENV
                     MGNLKQAGTNALSAIGQPLLEMMIPVFQTLATIVKGVAELFSSLPAPVKDFVVILGTV
                     VTAVGVIAPIFLSLQALAEFLKISIGEMIIAALPIIGTAIAIAAAVAAIIVIVKYLWE
                     TNEGFRDAVTTVWNAILEVINAVVSEISNFVMSIFGTVVAWWTENQELIRASAETVWN
                     AIYTVISTILEILGPLIQAGWDNIQLVITTAWEIIKTVVETAINVVLGIIQAVMQIIN
                     GDWSGAWETIKGVFSTVWQAIQSIVQTIFSAIQSYISNILNGISGTVSNIWNSIKDTV
                     SNVLNAISSTVSSVWEGIKSTISSAINGARDAVSSAIEAIKGLFNFNISWPHIPLPHF
                     YVSGSANPLDWLSQGVPSIGIEWYAKGGIMTKPTIFGMNGNNIMVGGEAGNEAVLPLN
                     DKTLGAIGRGIAQTMGGTSPTINITITGNTVREEADISRIADEVAQRIADELQRRRQL
                     RGGFA"
     gene            25751..26473
                     /locus_tag="ACL8CL_001340"
     CDS             25751..26473
                     /locus_tag="ACL8CL_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HGS1330582.1"
                     /translation="MIKHNELVIDGVRTSSFPFKIIVHDSPSIALGESKTALLEHGGV
                     SGAIVQTNKHRELVKKSYTIYLVKPTEEQMNQFMSLFIREKFWLESERVKTTRLWCYK
                     VNVTDLEEVQPGLYMTKATFTCHPTKYFKATDTQRLTRSGTLNVQGSALAFPKITIVG
                     QSVVETSFTIAGQVIRLEKLSESLVMVNNPDNPSFKTTTGKPVKWSGDFITVDPAKVK
                     NIGVVLGPGIQSIEIETVWGWA"
     gene            26474..>28005
                     /locus_tag="ACL8CL_001341"
     CDS             26474..>28005
                     /locus_tag="ACL8CL_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000966215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail spike protein"
                     /protein_id="HGS1330583.1"
                     /translation="MLYLLNKDVRTVRWNGEPLHEATSAIVKETMNGDFTLTVKYPIS
                     DSGIYQLIQEDMLIKAPTPVLGAQLFRIKKPVENNDHLEITAYHISDDVMQRSITPVS
                     VTSQSCGMALSRMVQNTKTALGDFSFNSDIQDRRTFNTTEIETLYSVLLDGKHSIVGT
                     WEGELVRDNFAMTVKKSRGENRGVVITTHKNLKDYQRTKNSQNVVTRIHARSTFKPEG
                     AEKETTIRVTVDSPLINSYPYINEKEYENNNAKSVEELQKWAQAKFSNEGIDKISDAI
                     KIEAYELDGQVVHMGDTVNLKSWKHNVDVFKKAIAYEFDALKEEYISLILDDKAGAGG
                     SRTSGGLSSAAYAILGVTESAQEVALEKALQNADLDFDHKAGLLRQEISDGIELAKAK
                     AEEVKQELSDTINQRFNSFDNGPLKEAKRKAEEALRNAGASSSLAQESKRIGLDSVAR
                     LEAFKSQTTSAQTALSGDLDALKRTIVNDIRPKQAQVEAEIAKQVEALVQTKKELSGA
                     STL"
BASE COUNT         8923 a         4708 c         6409 g         7965 t
ORIGIN      
        1 ggtatttttc ggattttggt cggggaattg gcggggactt ttttagcgaa tatgactaag
       61 aaataggtct gttgtcgctt cagcaagttc gtcctctact tgattgtaac gatccgtcat
      121 ataaaccttt gtatgcccca gcgcctggct taattgttca agcggaaccc ctgcaataat
      181 gctttgagtc gtgaagaagt ggcgcatcat gtgaggtgtt acatgcaatc ctgttgcttc
      241 attcactaga ttgaagtttc tatttagctg atttggattg atgagaccac ctttctcgtt
      301 gatagttata taatccttgt gctgttcctt gataattcct aactttcgct taatcttaga
      361 agcttcagct atcagataat agatcaggtc tgttccgata tcatcaaggc agacatatcg
      421 ctctgaatcc ttcgttttaa gccctccttt cccttttaag gtctggttgc ttcgactgtc
      481 tctaagatgc agtatagccc gtccgctgtc gttctgagtg atgtccattg gacgcaatcc
      541 aaagacttct cctcttctca atccaaaaat tgtcagatag gtcagagcgt agaattgttt
      601 tggcataatc tcttctgcct ttgctatcca agtcttgaac tctttgagag tcactttctt
      661 gtttgcagca gggatatcac tctggccgat gaaaacacct ttcaagcgat ttgagagcag
      721 attaccattt ttcacggcat cattcagcaa tgccatgaag ctggaattga gggtttgaac
      781 agtgtatctg gtatggttct gcaacttttc agcgataaat agttcatact catttctatc
      841 caaattttta agcaggatag aaccaaactt tgccttgata tggttcttat agagattgtc
      901 attgaggtag taggaagtgt cattccagcg ccctgttgac aatctctttt cggaatagat
      961 atcccaatat tgatcaagcg ttagattcgt attgatacct aattcttgtt cttggatttg
     1021 ttgctcaagc tctaccaagg ctgcacgagc ttgagggaga gttgtgaaac cacttttact
     1081 tttttctctt tttttacctc ggaagaaaaa agaacgtctg acatagtaac gcttgccttt
     1141 agcagtctca tagtaataga tatttgggta ttttgtttta ttatatttca ttgtattctc
     1201 cttgtttatc ggcttctgga caaggtctaa acattgagaa tattgacatc acccctttca
     1261 tggtgtaaaa tagggtatag aaaagaggcc tttttaatgg ctgatttttt ataagggtaa
     1321 gcttcacaat caaactttgg cgagggcgat tgtggggctt ttttttattt tctgaatagt
     1381 ttgtatagtt tatacaaact ccaagctgtc aaagggactg caaggaatgg agcgattgga
     1441 ataccaacaa ctccaaaaaa gactaagaaa agatacagtg ctgaaaatat gattttattg
     1501 gcaggaagtt ttttcttagg agatgtttct gaaagattaa tctcgtttac tatatcagcg
     1561 acactgtcac taacaatatt ttgttcaata tgttcagttt cagttgtttt agtttcaata
     1621 gcttcagttt ccgtaacttc tttattggaa atgttatcat caattatctc agatgattcg
     1681 ctctgaatta cttctttttg tgaagaatca acaatggtaa ggtaaatttc aaaaccaaca
     1741 tccaaatcat tatttgtgac aactttttct tcatactcgt cccattcttt atatgggcca
     1801 ccttttatct ctccctctat tacaaagtta tattttttat tatcaaaata ttttcgtatt
     1861 gttttagcaa tggttgcagg aacatatcct acatgattat caaatatcaa aacttttata
     1921 gcattaggat catatctatt atctacttct ggaacgagct caatattgaa aaagtctaaa
     1981 tcttgatatt tgaaaacttt cagaccatat tcttcaagtt cttctctaag ttccttagtt
     2041 gataaattgc tatagtactt tgcatattct gggattccgt tatcttcagc aatcatattg
     2101 caagcttctt ttactgcttt tttatatttt gtcactccag cgactctgaa taatattttt
     2161 tcaacagtct tttgcatata tttcccctct atataaatat cttcaatgca atttttaatt
     2221 tactaatgcc aagtactctt ccttgaccat gacttcatta gtcatagttt ttagattgta
     2281 gtaagacatg aatttgaggt aatcaaactc tgtggggtcg tctaagcttt ctagtgcgtc
     2341 tttcacgaga tgatggatca tattcctatc agcttcgttt tcacagcgta ggcgagcgtt
     2401 ttggtactct gagcgtgtat ggtctttgtg tccgagttcg tgtagggcga cttggatttg
     2461 ttgttcagga gctaagttgt ggtcaatagc gagtacgttt gtgtctggat tgtagaaacc
     2521 gctactgtgc cagtttgaac cgtcgaagag acaaagctct acttgatatt cttcgcagag
     2581 tttagcgagt gtcatagttc tccttcgtta tttataaaca tctctgcggt gggcgatttc
     2641 cacggctagg acgactagtt tatcgtcttg gatatcacag atgatgcggt aattctctac
     2701 tctgtatcgc cagtaacctg caaggttggc ttttagtgct tttccgtgtt gtcgtggatt
     2761 ggtcgtgttt tcaatgtttt tagcaagcca ggataggatt tgttttctgg ttggggtatc
     2821 tagtttttta agttgcttga gagctttttt atcaatatct agccgataca ttaagcaata
     2881 tcctctcgag atagtcctag ttcatccaag acctcatcca tggtataagt agctgggtcg
     2941 gctaaatact ccatataggc ttggtcggct gctcgtgcgt cttcgatatc ttccatgagt
     3001 accatgaagt cgtcaaaatc catggttgtt gtgtcgatac cgtgtttggt taggtagtcc
     3061 gtgatgtagg agtttttttc tgtgaaattg atcgtgatag tcattagcgt tctcctttgc
     3121 ttttgaagtg ggcggataga acggatgtga tgaagtcgat atcatcttca tttagtggtt
     3181 ttccgtcaaa taacatggtg ttggctgctg ctttgcgtaa gtctatgatt tgtccattta
     3241 cttgagcaaa ttcatcactt ccagcgatag caggattatc tgtccgtccg agtaggtagt
     3301 cagcacttac attaaaataa tcagctattt cttttaaaac ttttgaacta ggattacttc
     3361 tttttaatcg atataaagta ttagttccat aaccaagttt ttcttctaaa atatttatag
     3421 aaatcccctg tttatcagct aattctttta ttttatcgaa tgctacaaac attaattttt
     3481 caacctttct aagcaaacga aaaataattt taaaatattt gtagaaaata gttgacttat
     3541 ttagtcaaat gttttaaaat aatattcgta agctaaagag ttagcgaact agacaactaa
     3601 aaaataaagc cttacaaaac tgattggcgt ccgtttactg taggtaaaac ttacttttag
     3661 taggtctttt ctctatgttt ttattttaaa acatttgact agagttgtca agaagttcgc
     3721 taacttttta gataattttt taaaagaaag ggggagagga gaatggtaga gcctttctta
     3781 attggctttg ttttcggtag ttttctgtgc tacattgtcg ctactttgat aggtgctgta
     3841 ttggacttta aacttcagaa gtggcaacaa caaagcgata aaagcaatcc aacctgtaag
     3901 gattgaaaac tgtaataaag aataagaacg aatacaatag caaggattct ttgttctgta
     3961 aaagctttca agaaatctgt taacagtgaa tcagaaatag gatagaaggt agtaaaaagg
     4021 tgaaatccta cgataactgt caaaatcgga actaggacac catataagat gatttccttt
     4081 agatagtgaa aaaatgaaga tttcattatt ctcctccaat cgtttttatt ttaattatat
     4141 caaatttaga aaggaaaaat atgagtaaag aactaaagat aatcaaggct aaaatcaaaa
     4201 ctcgtttgat tgagctggat atgactcaag ccgaattggc aaaacaagta tctgtaacat
     4261 catcagttat ttcagaactg ctgaagtatg gaaaaggaag tgattatgtg aaagaaaaag
     4321 tcgtagatat tttgggtatt gaaaaccctt gggaaaatag ttagagcatg aggaaatagt
     4381 atgaacaacg cagcgcaaaa agtaacacgg attgacaaag atgcctggga gattgctacg
     4441 gagttggcga acgagtacgg ggtatctatt tgtcacatca tcagtgagag cgtccgctac
     4501 tgtgcagaga atgccgaatt taaggagatg gacgttgtcg ttaaacgatt ggtagtcggc
     4561 agtaaggtgc tggagtagga gggtggagat gaatgaacta gtatggtttt actttgttgt
     4621 cataatcaat cttatcattg gttttgctac atactacgct agcaaaagag atagagaaaa
     4681 gcgcatcaac gggtttaaaa aaatacaaga tgatgagctt gaaagagtta gaaataaatt
     4741 taatttatga ttttttagaa gtcttttgaa taaatctttt atttaagttt tgtttgctat
     4801 tgcttgcttt attagctatt tttaaagctt tgtcaaaatc aacctcgcct gtaaaaactt
     4861 tgtaggtaag atcattcatc ttcatagact tgtcggtttc agtatcaagc tgagatacct
     4921 tctcaagttc ttgcaaccgt aattcatgag cttgtttgat tttttcaagt tcaaaggcat
     4981 gttgctgttt tagagtatct atctgatgat gaaattctct ttcgagtttt tcaacagtat
     5041 gtgaatgttc tttggcttgt ttttcaatct cagctttatt gttagctttt gatgcgacat
     5101 atgaccataa ccctgatatt attgcaagaa taatactaat cacaggttga ataagagctt
     5161 gatattccat aagatttctc caatcgtttt attttaatta taccaaattt agaaaggaac
     5221 tttatgaacg aaatttttaa ttttcacgga caggaagtcc gtactttgac aattgataac
     5281 gagccttggt tcgtcgggaa agatgtcgca gacatcctag gatatagtaa ggctagaaat
     5341 gcgattgctc ttcatgttga tgaagaggac gccctaaaac agggcatccc tactagtggt
     5401 ggaacacagg atatgttgat catcaatgaa tctggtctct actctctcat tttatccagc
     5461 aaattgcttc aagcaaaaga gttcaagcgt tgggtgacat cagaggtctt gccagctatt
     5521 agaaaacaag gcggatttat ccgagaggac ttggatgaag atgctttcat cgctctgttt
     5581 actggacaga agaagttgcg tgagcaacaa gcgaccatgc tggaagatat tgactacctc
     5641 aagagtgagc aaccgattca tccaagctat gctcagtcgc tactgaagaa gcgcaaagct
     5701 cgggtcgtgg cttgccttgg cggtattgac agtcctgctt atgcggataa ggttttttct
     5761 caatcagtct ttagacaagc tgagattgat ttcaaagacc acttcaacat tagtcgctat
     5821 gacttgctac ccaagaagca tgcggatgcc gctcttgctt actggatgac gtgggagcca
     5881 agcactaata ccaagatgaa aatcatgaaa ttgaactcat ttgatgacgt ctagaaaggg
     5941 gaagaagatg gacaatgttc tactttcact gtctgaatgg attaaatcta ttatcaagga
     6001 cacaatcaca aggctagtcg aaatagaaaa agatagtgat cactatccag agttgatgga
     6061 tgtgaacact acctgcgaat ttctaggaat taagtatgcc accttttcag ataattatcg
     6121 ttacttaaag ggatttccaa aggaattacc tggtaagaaa tggtcaaaaa gagccatcaa
     6181 agaatggctc tctaatcaaa tataataact ttactaaaag gcttctggac aaggtcttag
     6241 caaaattatt tgactatatt atagcacaaa aagaggataa ggagataaaa atgtttgaac
     6301 caccgatttt agaccagctg atgggggttg gagccttact gcttggattt gcaggggctt
     6361 gccgtcatat caaattgcag gaacaacgca aggaagaaga aagacgagaa gagcaagaat
     6421 ttgcgtctat gattatccaa gtgcgtaatc atgcatatga acgtggtaga gaggacaaat
     6481 ggcaagaaat tcgcaagaat attcgcagag agttcaaagg attcacatat gacaacgaac
     6541 cgcccgtagg attgcgtcct gagccgttag ctttgccaga acctaaacag tctgcaatca
     6601 gacttctgta atgaggaggg caggaaatgg aagaattgat tgaatggctt gaaaccaaag
     6661 aaaaattaaa cagggaaatg atttctgacg aggaaacatc ggactttaac ctacatctag
     6721 aaaatgataa tgataccatg ttcatgatca aggaatactt gaacgattat aacaagctag
     6781 ccaaagacta tcgaattcta tcgtctgaaa atcgtctact taagcttgaa aaaatggagc
     6841 tggaaggcag gtacatctat gaggatatgc gaatgaagta tcgtgctaac cgtaggaagt
     6901 ggggtgctag gtatgtctga aattaagtgg ataaaaatca caaccgatat ttttgacgat
     6961 gaaaagattt gcctgattga tgccttgcct gatcctgatg ccatcttagt gatatggttc
     7021 aagattttga cacttgctgg aaaacataac agtaatggtt tgttgatgat gactgataag
     7081 gttcactata cagatgaaat gttagctact atttttcgta gaccattgaa tacagtaaga
     7141 atggctattg gagtttttga acagtttggg atgattgaga ttatcgatgg tattattagc
     7201 ttgccaaatt gggaaaaaca tcaaaacgtt gacggaatgg agaaaatcaa ggaacagaca
     7261 cgtaaccgtg tagccaaata ccgtaaaaaa cagaaaaatc ttgctcttgg taacgttaca
     7321 ggtaacgtta cagtaacgga cggtaacgca ctagaagaag ataaagataa gaatagatta
     7381 gataaagata agaataagaa aagaataact actactagta gtggtagtga agaaaatatc
     7441 ttagaacttt ttcaatctga gtttcgtaga ctcttatctg gatttgaaat tgaagaaatc
     7501 aaccatctac taaatgagaa tgatgtggat ttggtgaaag aagcattgaa gactgctatt
     7561 aactcaggaa agccgaacat caaatatatt ggtgggattt taagaaattg gcagatgaac
     7621 aatgttacca ctgttgaaca ggttcgtcaa tcggaaaaga agaacaagga taagaaagaa
     7681 gaacaggagg ccaaggacga atgggggtac tagaactaat tgaacaattc gagattgact
     7741 attatccgtt aagctacgag aagaaaactc ttttagcaga ccaaccaatt catcaagtgg
     7801 ttgcctgctt gtctgaaatg gctagctggc atgaatgcgg aggtcggcta gtatggtaga
     7861 caatgtgttt gaggaaatcg ccttatctta tcgtaggaat acagaacaac aagaagagtt
     7921 ctgcgaaaag cataacatcc ctttgataaa gatattgagg accgagagtg ttgtatgtcg
     7981 catgtgtgaa tctgagcgga ttcatgaaga aaatcaggaa agagtgaatg aactggctaa
     8041 tgctgagaat gagcgagaga ggaaatacta tcttgaaaag ttctctcttt atgatgaagt
     8101 tttgaaaaat gctactttgg acaattttga aacaccaacc gaaaaagaag cggaaaagct
     8161 agcttttgca aagaggattt gtcgtgagtg gtctgagggt gctaggaaca acatcgtgtt
     8221 acaaggagaa gctggaacag gtaagagcca tttggctttt gctgtggtta aggctctatc
     8281 tgagtacacg aaagagattg ctatttttat taacgtaact gactttctga tgaagattaa
     8341 agctgatttt agtcaggaag agtttctggt caataaaatt gctagtgcta agttcttggt
     8401 tttggatgat ttaggaatgg aaaaagatag cgaatggtca tttactattc tctacaatat
     8461 cttgaataag cgttcaaaca caatcattac cacaaatttg atttctgccg atattcaaaa
     8521 aagatatggc agacctttca tgtccagact aatgaagggt gtagataaag accatttgat
     8581 ggttttcaat gatttgacaa acaagcgaaa gcaatatttt tagaataggg gtggctgatg
     8641 tttattttaa agcatgggac aagagaggat aagccgtttc taaggtctgc aattatcagt
     8701 gtgactggct tggatatttc gtgttctgag gagaagaaag ccatgcggtt tatttctcgg
     8761 gcggcagcct tacaggttag caaggcattg agaggttcct ttgggaactt ctaccctgtc
     8821 gaggtggagt gatgttagag ctttacttcg tctacaacgg gcactgcaag ttttaccttg
     8881 gaacgtttga caatgtcgat gatctcattg aacagatgga agatcatcag tgggctttct
     8941 cggctatcac tcatccaaga tttcagaagc acattggtca gcggacgaca cgatttgact
     9001 acggttctaa ggattgttac tatttagcga ctttttcagg aggagaaaaa aatgattgaa
     9061 cttattaaag aatttggaat ggctattctg tggttatttc tcggctattt agtcggggaa
     9121 cgtgcagcaa gaaaggaaaa gaaagatgat caataacgtt acatttttag tgggaggtaa
     9181 gaaacatggt tggagtaacc tatcaggaaa ttcatctctt tgttgaattt tttgaaagag
     9241 cagtacgggc aaggtcgtcc agactatatt gaagccctga acgacttaga cggtctggtg
     9301 gaagtctcct acagagaagc tattgaaaga tttttagaag atgaagtacg ataaacaggc
     9361 tgagattgac ggactgaaac gcacgatcga gcaaaacgaa gagaagataa tcgagtattc
     9421 gaagccgtgc gatgcacgca agagacggat tagagcgctg gagcgcgatt tgttgaggaa
     9481 aaagaataaa gaattgagac ggaaagtgga ggagttggaa ggtaatggaa gagttaaatc
     9541 aaaaagttaa tgcagtatac aactggacgg tagaagacgg gaagcccaaa cctcccaagc
     9601 aagatttacc acaagcggtg aaagaccggg cggactattt ttgggaaatg gcagaagatg
     9661 gtatgacgtt tatgggagcg atggaatgca tcttcgctga tgaaaagcct acagactatg
     9721 aattgggagc tactaagggt tggttgccaa aatctaagga gtttgatgat tgggttggct
     9781 attcgccaag catgactcag gtagttattg cagtttattt gatttatgga ggaagcgaag
     9841 atgaaactta atgaattgat taagaaatat aaaaaacttg agggtgtatg gaatgctgaa
     9901 ggagcagaac tagctcgtca aatttttctg caagacttgg aacaactaga taaacagcaa
     9961 ccagtcaaag ttaagaagtt tgtggcggat tttatcgcag aacagaaaaa actgggtcat
    10021 acactgtcct actcaataga cgcaagcatg tctgacatag ttgcagaatg gtattgggat
    10081 aattccgaac tcttcgcact agcttggatt ttcggctgcg aggtcgagga agagaagcgg
    10141 tatacagtag tgacgaaagc aacaaaacaa ccgctatatt ataatgctat ggataagaaa
    10201 ctattcttct ctatgggcgg actagctaca aaatttactc gcaaacaact caaagaagcg
    10261 gacttcggct gggtgtttga ttgtccaggg attgagattg aggaggtgga gtgatgacac
    10321 aaacacttga agaaggaatg aagaatcaaa gtaaatgcat aaaagtccca agggaaatca
    10381 gaccgtttga tatagggtat cgaatagtga acaaatacgg tcaagcgctc gctttaagaa
    10441 atggggcaag tatattcgat ttgccttttc tggctgaaaa agctatagaa aaagagtttg
    10501 ggaagaatga tccagacttt gacatcggaa agcattctgt tgaagaggtc gctattgtca
    10561 atttaagtaa atttcatagt tattttgagg aggtggagtg atggaagatg agcaaaatat
    10621 tttagagata caattgattt taggtaagca agttttagaa attgtattgg atttgctaaa
    10681 agacgattca aaaacagggg tagttctacc tttaaacata aatgaccgtg aatttacaat
    10741 tactgtagaa aaggaggtca cagatcgtga ctaaggggtg cttgtggtta tgaaggacgt
    10801 gtatgacaag gtgaaagtcg gggatgaggt gatgttgtga aattcttgga tttattcgca
    10861 ggaattggcg gttttcgttt aggaatggag tctgccggcc ataaatgtat tggcttttgt
    10921 gaaattgaca agtttgctag agaaagctat aaagcgatac acaatacgaa aggagaaata
    10981 gaattacatg acatcacagc agtatcagat gaatctattc gaggaatcgg aagtgtggac
    11041 attatctgtg gaggatttcc gtgccaagct ttctcaattg caggaaacag acgaggtttt
    11101 gaagatacac gaggaacttt gttctttgaa attgctaggt tcgcatctat tctcagacct
    11161 aaatatctat tccttgagaa tgttaaagga ttgctcaatc acgaaaatgg agttacattc
    11221 gagaccatta tctcaacctt ggatgaactg gggtacaacg tggaatggca agtgcttaac
    11281 agcaagaatt tcggagtccc ccaaaatcgg gaacgagtgt tcattatcgg acattctaga
    11341 aaaagaggca ccagaagagt ttttcctatc ggaagagcaa atagagaatt tgattttgaa
    11401 ccaaaaataa agattgttgg taacactaaa aatccgaacg ggacaagtca agggactggg
    11461 agcgttgttt acgactcaaa cggtttagtc ggtacgcttt gcgctagaga ttataaagag
    11521 ccaaaacaag tagctatacc agtattgaca cctgatagag taaataaacg acagaatggt
    11581 agacgtttaa aaaaaacggt gagcctatgt ttacgctgac ggcacaagac cgtcatggga
    11641 ttttaattaa agaagcaaca aaaaaaggtt atgcagaggc tacagttggt gatagtgtaa
    11701 acctatctca tccaaactct aaaacaaggc gaggtagagt tggtaatcag atagcaaata
    11761 ctctcttaac tggagagagt caaggtgtgg ttgagcctga ttttaggatt aggaagctaa
    11821 cacctaaaga atgctggagg ttacaaggtt ttcctgattg ggcttttgac aaagcgcaag
    11881 aggtcaactc taacagtcaa ttatataaac aagcaggaaa tagcgtgaca gttaatgtca
    11941 tagcagcaat agcaaaggag ttatcatgaa cacaatagag aaagtcaaac aatggtttat
    12001 tgaccgtgat ttagaaaacg gtagggtcgt tggattgatg gtgctttcgt caaagaggag
    12061 gatttataaa atgaaaagac taggaatcat tattggggcg gtatttgtaa tcgttgtatc
    12121 gccgtttgta gttcagtatg gatggaatga gattatcaca acaattgttc cagttggtaa
    12181 aattacagtc tggcaagcat tagggatgga tgcactacta tctttcatct ggcctgtgct
    12241 atctagcaaa aaagaatctg aagaggatta ttcgtatgcg gtaaagagca gtatttcaaa
    12301 aatcattaca tgtgcatttt tgatatggtt agctagtttg tttatttaag gagtgttagc
    12361 atgataccaa aatttagagg gttatccatt gacgaaaaaa gcaaaggaga atggcaatac
    12421 ggacatttaa ttgaagatag aggaagagca tttattatca acgaagtggt agaagccaat
    12481 gaacaataca ttactatagg ttcttggtgt cctgtaaatc cagcaacatt aggacaatca
    12541 acaggactca gagacaagaa cggcaaggag gtattcatcg gtgacatcgt taaatgtaca
    12601 agaggatgtc tccatgaagt atatttagaa aaagaatacg gtggcacata cataggtgga
    12661 atgcctgctg tatacctaaa aggatttgga gatggatatg cgtggacgga atatgaggaa
    12721 atcatcggca atgtctacga aaatccggag cttttggagg tcaacgagtg agatatttta
    12781 aaatcctatg tttggtatta ctgaacaaac agttagattt tatggaacga agacaagtag
    12841 gaatagatat ccgatctcga caattttgat aacttgttta catttttgaa aaacttggag
    12901 gtggaaattg aaaaaattga gcgacgaaga cctcaaaaca ttagacagag aacttttcaa
    12961 atttcaaaac attcaacgta caatagattt gagaaggcta gaattagaaa ctcgaaaccc
    13021 agatgctcag agtggtccca gcgtaggaat aagcaaacct accgaaacta tcgcagtcag
    13081 aatcgcagat gatccaacct taaaatttct cgaagggttc aaagctatta ttaacaaact
    13141 cttgatcaat ctagttgatg aagataagga aatctttaat ctgcgctgga gatatcctca
    13201 actgagatgg gaagaaatag cagaacagaa attcatgagc aaagctacaa tctatcgacg
    13261 tagaaggatt atcttagaac agtacgctat actgaaaggt gagttgtaaa taagattgag
    13321 acaaaagaca tcttgaagtc tcacaaaaaa aggtctatta tgatagcatg aacttctgaa
    13381 acaaaaacac acatcacact tgaggagtca tccttaattc tagtcaaaaa gttgtccaac
    13441 agaagtatcg tcaagagtca gcaaatgctg gctttttgtt tttggaaagg aggtagaata
    13501 tggaatttgt atcaccgata aaagataatg acgacattca ggcaatgaaa gattatctta
    13561 gagagtggaa tgagatgtat tatatgctat tcattacagg cctgaatact ggtttgcgag
    13621 tcggagatat acttaccttg aaagttaaag atgttcaagg ctggcacatc aaactgagag
    13681 aacggaagac tggcaagcag ataacaagac ggatgacaaa agaactcaag aaagaaatga
    13741 ggagatatgt cgagggcaaa ccatttcatc atttcttatt caagagtagg caaggtcaga
    13801 ataaagcaat cactcgtgag cgagcctatc aaatcataca tgaagcagct gaagaacttg
    13861 gcattgataa tgttggcaca catacaatgc gcaagacatt cggctataaa tattacaaca
    13921 agacaaagga cgtagggaca ttacagaaaa tgttcaatca ctcatcacct gcaattaccc
    13981 tgagatacat agggatagaa caagcagagc tcgatgatgc tttacggaac tttgtcattt
    14041 aattttttta gatattactt tcacataatg agttaagcat aaactgaaaa aatgaaactc
    14101 tttaaaaccc atgcttagta agggtttgag atttagagtg agtttaacaa aatataagat
    14161 atgtgaaagt gagggataaa attggtatag ttacaagagg tgaaaaatgg taaaagaata
    14221 ccgtatttgt aaatggaatg catataccgt taacagccat actagtaaaa tggaataatt
    14281 tttttaaaga atcagaatga gacaaaagac atcttgaagt ctcacaaaaa aaggtttatt
    14341 atggtagcat agatttcttg tatgaggagg ggataggtca aaggcctgtc ccttttagca
    14401 ttgagaaagg aggtttgaga tgtataacaa acctatcaga ccatccttga aatctaagaa
    14461 gtgggagaag ttccgtgata ggataatgcg taagcatgat tatctttgcc aagaaagttt
    14521 gcgttacgga atttctgttc aagcagaaat ggtacatcat atctttcctg tgtctgaata
    14581 tcctgagctt gaatttgttg aatggaattg cttgccattg acgaataaga aacataatac
    14641 gtttcatgat agagtgaacg atagagtaat caaccaagga ttgtattggc agaaaaaaag
    14701 aaaaaaagaa tttttaaatt ttttcaaaaa tgaaaaatga aaatttttaa tcccccctct
    14761 ttttgaaaaa tcattttggc cagtagggta ccggtgaagg gaactttttc caagtcgggg
    14821 gccttcaaac aaaaaggggg taaaaactaa gcgattttga cgaaaggagg tagtttttgg
    14881 ctaaaccaat tacagcaaag tcgattaagt caaaagtggt caagcagatg aaagacttgg
    14941 gaacttatcg taaagagttc gaaatgatca ttgacatttt tgcaggtatg ttataccagt
    15001 atcagaaact tgctcaagat tatgctgata tgggttatcc agtaacagac acctacgtca
    15061 ataaggctgg tgctgaaaat gagcgcaaag ttccaatctt gacagcgatg gaaattttga
    15121 ggaaagacat actcagctac tctaatcagt tgatgatgaa tcctaagtcg ctcggtgagg
    15181 tagtagaaca agagggtgag tcagttctta ctgaggtact gaagttcaag aacgaaatca
    15241 agaagaagcg agtgactggc aatgggtaat cttgataaag ctaaagaata cgctcaacac
    15301 gtcttaactc accgagaaga acattgcgaa gaaaacatcc ttgctgctga acgttttttc
    15361 cgtgatttag aaaatccaat ctttgagatg gatgaggata tggtggattt tgttattcac
    15421 tttatcgaga acgtgatagt tcatcagcag ggcgatgata tgtttgcggt atctatccgt
    15481 aacaagccat tgcttttgca accgtggcaa catttcgttg tagttaatct gtttggtttt
    15541 tactacaagg gtacgaatga gcgcaggttc aaagaagcgc ttatcatgct tgctcggaag
    15601 aatgggaaga cctcgtttac tgctgcaatc gcacttgctt atcagatatt agatacagat
    15661 agcggttcca aatgctatat cgttgctaac tcagtcaagc aagcgatgga agcttttggt
    15721 tttttaagat tcaacgttga acgctggaac gataagaaca ttcgtatcaa ggataataac
    15781 caagaacatt ctattactgc caattttggt gatgaaggtt ctttctttat ccaggcttta
    15841 gcgaatgatg agagccgtct ggactctttg aacggaaatg ttatcatctt ggacgaagct
    15901 cacacaatga ggaacagtaa gaaacatggt cttatgaaaa aaacaatgtc agcataccgt
    15961 aacagtatgc tttttgttat ctctacagct ggggatattc ctaccgggtt ccttgctaat
    16021 cgtttgaaat actgtcaaaa ggtgctcaag caattggtca ctgatgattc atttttcatc
    16081 ttcatctgca aggctaatca atctgctgat ggggatgtag tggactatct ggacgagaac
    16141 atcctcaaga tggctaatcc gtcatgggag gtcacggttt cgctcaaggc tctcaaggaa
    16201 gaagcagagc aggctatgaa tgatcctcag acaagaaatg agtttttcaa taagaccttg
    16261 aatatcttta caaactctat gaacgcttat ttcaatccgg atgagtttat tgcgtcggat
    16321 agttgttacg attggagtct agaagagctg gcacgcttgc cgattcgttg gtatggtggt
    16381 gcggacttgt caagattgca cgacttaaca gcagctgctc tctatggtgt ctataatgat
    16441 ggtgagaaag acgttgatat ctgtatcaca catgctttct ttcctcggat taatgctcag
    16501 aagaaggcta acgatgatgg gattccactt tttggctggc agtctgatgg ttggttgacg
    16561 atgagcaaca ctccaacggt actctatgac gatatcgtca aatggttcat cagtatgcgt
    16621 gagcgtggat ttaaaatcca agctgtgggg atggatagga agtttggtcg cgagttttta
    16681 gctaagatga aaaaggctaa gttcaagatg attgaccagc ctcagttatt ctatctgaaa
    16741 tctgaggggt tcagacggat tgagttcaaa gtcaagaaca aggaatttta ctatcttcat
    16801 tctgacgctt atgaatactg tgtgagcaac gttagagcaa ttgaaaaggt ggacgatgct
    16861 gtgcaatatg agaaattaga tggagacggt gggactgcaa ggattgactt gtttgatgcc
    16921 agcgtctttg cttgtataca ggctcttgct aatcttggca agggtggtga tgtgatgaga
    16981 ttctttgatt aggtgaatta tgaatgaaat agtattatca gaacatgaaa ttaacttgct
    17041 gatcaacaaa gggcgagtta aagtaatttt aaacggggaa gtagtaacta ttcgtcaaag
    17101 acatatgaaa aatttgatgg ctgaaacagt aaaatgggaa aaacaggtaa ttgatgtcag
    17161 tcagaatatc gtaagaaata aacactttga ttcacttttt caaaatactt ttcgttagaa
    17221 aggaggtgag gaaacatggg tatttttgaa aagttttgga aacgaaacaa gccaagtaag
    17281 tcaatcaaca tgctgagtca ttcagattta gggttgtcaa accttatgga ttcgtatgta
    17341 cctttggcca gaaatccaga tgtggtgaca gcggttaata agattgctga tttggtctct
    17401 aatatgacca tccatctgat ggaaaacacg gataaaggtg atatcagaat tcgtgacgga
    17461 cttgctagaa agattgacat taatccgtgt gaacacatga caaggaagtc atggattttc
    17521 aagattgtgc gcgatttgct tttatatggc gatgggaact ctgtcctaca tgtggaatat
    17581 gaacctgtta cggattatat ttctaatcta agaccatttc cgatgagaga ggtttcattc
    17641 caaacagata aggattccta tgtaatctca tttaggggtg aagaatattc ccctgatgaa
    17701 gtagtccact tcgtcatcaa tccagatcca gatattccat acattggtac tggttttagg
    17761 gtgacgttga cagatgtggt tcaaagtttg aacatggcta ccaagactaa aaaaagcttt
    17821 atgaacggga agaatattcc tagtcttatc gttaaagtag actcgtctag tgctgaacta
    17881 gattcggagc aagggcgtga gcgtatcgct gagaagtatt tgagtactag cagggttggc
    17941 gctccatgga ttgttccaga ggcattgctg gacattcagc aggtaaaacc gcttagtcta
    18001 acggacatcg ctttaaacga gtctgtggaa ttggataaaa gaacagttgc aggtctatta
    18061 ggagtacctg cttttatttt gggtgtagga gaattcaaca agacagagta taacaacttt
    18121 gtaaatacga ctgtcatgag tattgctacc actattactc aaacactaac cagggactta
    18181 cttttgtcta gcaatcgtta cttcaagcta aatcctcgtt cactcttctc ttacaacatt
    18241 acggagttgt ctgctgttgc tcaacaaatg gcaaacagtg ctgcaatgcg tcgtaatgag
    18301 tggagggatt ggctagggat ggctcctgat cctgagatgg aagagttgat tgttcttgaa
    18361 aactttctcc ctcaggagaa actaggagat caaaataaac tgaagggagg tgaggaagag
    18421 aatgcaaaag cgaaatagct atcgtgccac tcaatttcaa acgagagaag aagaatctgg
    18481 tgatttgatt ttgagtggct actttatcaa gtttgacgag gagacggaat tgtggccagg
    18541 ctactgtgaa gttatcaagc gtgctggtgt tgagaaagct gtcacagacg ctgatatcag
    18601 agctttattt aaccatgatg atagccttgt tctcggtcga acaggtaacg gaactctgac
    18661 actgggtgtt gatgatgttg gtctttttgg agatatcatc attaacaagg atgatcctca
    18721 agctgttggg gcctatgccc gtgtcaagcg tggagatgtt atagggtgta gctttggctt
    18781 tatcccggta aaaatcgaaa cggaagaacg tgaagatggt tcatatttgg acactgtctt
    18841 agagctagaa atctttgaag tgagtccatg tactttccca gcctatccac aaacggaaat
    18901 tgctgcacga caaaaagact tcgaaagtca gaagcgtgct aatcgtgaag cgctagacaa
    18961 gcgcaagaaa gaaattaagg agaaatttaa gctatgaata aggcattaat ctttggtgct
    19021 cgtatgcgag caaaagcaac taaggtagtt gagttggaag aaactatcga agaattgaac
    19081 aaacgttcgg ttgttgagtt agaaaagttg gatcgtgcta aaaatgatga agaagtttta
    19141 gcagttgaaa agactgtaga cggtcttcaa agggaaattg aagaaaaaga agctgaaaaa
    19201 gtacagcttg aaaacgagat cgatgagtta gataaacaaa tcaaagagca aaatcgtaaa
    19261 gcaccaactc cggggaaaat ggaagaacga ggaggaaaga cattgggaca acgtgaagca
    19321 tttaatcatt acctacgaac aaaagaagcg cgtgctgatg gtttcaaatc tgccgaaggg
    19381 gaagcaatca ttcctgttga attgatgacg cctaaagaag cgaaacaaga caagacagat
    19441 ttgacttcat tggtcaacat cgttaatgtc aagaacgcaa gcggtaaatg gtcagttgta
    19501 aaattgactg atcaagcaat gaacactgtt gaagagttgg aagaaaaccc tgaattggct
    19561 aaaccaacct ttacaaaggt gaactatgaa atcaaaacac gtcgtggtca tttgccagta
    19621 tctcaagaat ttattgatga cgctgactac gatgtcatgg gattggttgc taaacaaact
    19681 aagaaccaag aacgtatcac taagaataaa gagatcgcta aagttctcaa gacagctaca
    19741 tctaaaagcg cagctggttt ggacggcttg aaagatattc tcaacgtgga attgaaaaca
    19801 tactacaatg caactatcgt atgtactcaa tctatgttcg ctgctcttga taaaatcaag
    19861 gacaaggacg gtcgctacat gctccaaaca gatatcacat ctccaactgg ctacaagttt
    19921 gctggtcgtg ttattgttgt ttatccagat gatatcattg gagaaagcaa gggagatttg
    19981 aaagccttca tcggtgacgt tggagaattt gcgacattgt ttgaccgtgc gcagactact
    20041 gtcaaatggc aagatgacaa aatctacggt cagtacctag gaactgcaaa ccgtttcgat
    20101 gttaagaaag ttgatgaagc agcaggcttc tatgtaactt acactgacgc tgtagggtaa
    20161 ggaggtagcg tatgagctat aaagtaatcc gtcctttcaa ggacttggct gatcctgaaa
    20221 atcatgacta cgctgttggc gatatctttc ctcgtgaggg atatgagcca acagatagct
    20281 ttaccaatgg ccttttgact ggcgctaaca ctgctggctc tatcttcctt gaggttttgg
    20341 gagatgatga acctaagaaa ccagcgcctg aaacaaaaga agtgaaggaa gagcccgcag
    20401 ttgagcagga agaaacagtt gaggaaactg ctgaagagtc tgctgaggaa gttgaggaat
    20461 agacatggac caaggtcagc ttttagagtt gctgaagctt aagttgggta tttcaaccga
    20521 cttgagagac aagccgttaa aaaaaatcat ttcaagtgtc atcactgaat tgaccgataa
    20581 tctcggtatc gagcttgttg gtgagcgtgc tgaccatgaa atgtttatcg ttgactatgc
    20641 cgcttatcgt tatgagggtg gggtggatat gccacgtcac cttcagtggc gactgcataa
    20701 tttacagata gcatcaaaga aagaggtcaa gaatgtggaa tcatgaaatc acactgatct
    20761 ccaagaaagt aacaggtaag gataagttac tacaaccaat ctctgaagat gttgaagtta
    20821 ctctcttatg tcgcaaaaag agggtcactc gctctgaatt ttatcaggcg aatcaggtag
    20881 ggcttaaacc gagcttggtc gttgagattc gaaattttga gtatgagaat caagagcttg
    20941 cgaagttcga aggcaagcaa tatcatatct tgaaaaccta tcctatcgat tctgaaattt
    21001 tagagttgac tttgtcagag gtgttgaaat gagtaatgac cttgctgatt tgatagcgaa
    21061 agagcttgca gcttactctg atgaggttac tgaagaagtg gataagattg cagagcaagt
    21121 ggctgatgag actgtggatg agttgaaaga gacaagtccg aaacggtacg gaaagtatcg
    21181 tagaagttgg aaaaagaaga agttggccaa tggctctttt gttgtgttca acgcagttgc
    21241 aagtcttact cacatacttg aaaacgggca cctttcaaga aatggtggtc gtgtcgctgg
    21301 tatcgtccac atcaagccag ctgaagaaaa agcaattcag aactttgaga agcgaatcaa
    21361 ggagattggg aaatgaagct atcagacttt gctgctattt tggaacaggc aaacttgcct
    21421 gtcacttatc gagcgtttaa aattggaaac gcacctgacc taccttacct ggtctattat
    21481 gaatcaagtc cagtcatcaa ttcagctgac aacacggtta atcatcagat taagagcgtg
    21541 acagttgagc tggcttttga gagtaaggat gaagatttgg aagaacgtct ggaagagctg
    21601 tgggcaaacc acaagctctt ttttgaagtt caagaagaaa catttatcga gactgaaaga
    21661 ctctatgtca agtcttacac agtctatcta tactaaggag gaatgacatg actcaagaaa
    21721 ataaagtaac ctttggccta gaaaatgtac atatcgcacc tatcaagaca cttgcagcag
    21781 atggagttat cacttacggc gatgtttttc gttttcctgg agcaatagag ctgatacttg
    21841 atactaaagg ggaaacaacc cctatcaaag cagacaacaa ggattaccat ttcatgaatt
    21901 caaacgaagg atatgagggt aaacttaaaa tcccacacat cattgatgaa tttgcgacaa
    21961 aaattcttgg tgaaatcaag gaccctcaaa ctggtgttat gactgaaaaa gcagatgcga
    22021 gcttgacaga gttcgcaatg atgttccagt ttgaaggcga caaaaacaag actcgctatg
    22081 tgatgtacta ctgttttgcc agtcgcccat ctcttggttc aaaaactaag aacgggacat
    22141 caaccaacga acgtgaactt agtttcaaag ctagcccgcg tccattggat acagttgtca
    22201 agcgttctat cacatcagct gatgacaagg atgcgtatga caactggttc aagaaagtgt
    22261 atgaacctac tgcagttgca gcttaaggag aagatctatg cgtaaaatcg ttttggttgg
    22321 tgatcaggag tatgagttgg ggaccaacgg ctatactcct attgcttaca agcaacaatt
    22381 tggaaaagat tattttcaag atttgttctc gatgttgaaa aatcaatcat tcatgaatga
    22441 attgaacaag ttggaaaccg acaaggaatt gacagcgact aatattgata tttcgatgtt
    22501 gtcagatttt gacatgacct ttttcaaccg tcttttttgg acctttgcta aatctgcaaa
    22561 tcctcacatc aagccttatg aacaattctt catggaaatg gaaatcttcc cgattcagga
    22621 agttggacct gtgctgatgg aaatgctaaa tgcgagcatg acgacaaaaa agcaccagat
    22681 gagtcagaat cagctagcga agaaatcttc acagtagaat cctatctatc ttgctgtaaa
    22741 gaaactggtc tttctattga tgatctaaag cacatttcaa tcggaatggc tctggattat
    22801 cagacggatt atgtgaatct acggagcgag gataagggtg gcgaacggaa agccacgcaa
    22861 gctgattttg acggttttta aagaaaaatg agtgctgaga gagcgattct gagaccaagt
    22921 tccttggtct ggctgcatta tcagtggtag aagttctctc agcgcttttc tatttttttg
    22981 agaaaggagg aaatatggca ggaaatatca aaggtattaa aattgaaatc gatggcgaca
    23041 cgcaaccctt acagaaggcg ctgaaaaatg tcaataaggc tgctactgat gcaagtcagg
    23101 agttgagaca gattgacaaa gcattgaagt ttgatacagg gaacgtaacg ctcctgactc
    23161 agaagcaaga ggtcttgcaa aagcaagttt cgacgaccaa agagaaacta gaaaccttga
    23221 gacaagctca gtctcaggtg gaacagcaat tcaaaaatgg cgatatcggt gctgaccagt
    23281 accgtgcttt ccaacgtgaa gtagaagtta ctcaaaacgt cctaaaagga tatgagggta
    23341 agcttgcaaa tgtgaatcag gcgcttaatg agaatgggag tgctactcag aacaacaaga
    23401 accaattaaa agagttgcaa aatgagcaga gtcaacttgc ttcagagatg agtaaggtga
    23461 caagttcatt caaactgcaa gaaagcgctt tgggttcaaa tgctagtgaa gccgagagaa
    23521 atgctcttgc tcagaaaaag attggtgctc agtctgagat tgtaagtaaa cagatttcaa
    23581 atctagaaca gcaattggaa atcactaaaa aagaatttgg tgagaactcc acacaagcca
    23641 acaggatgga agcagagcta aatcaggcta agacagcatt caatcatctc aatgatgaga
    23701 tgaaaggaac aaagtctgct gctgatagca ctcaagaaag tttaagtgaa atctcaagaa
    23761 atttaagagc agaactactt caacagttta gtgagaagtt gagtgctatt tcagaaaagc
    23821 ttgtggaagt aggaaaagaa gcgttagaag cagctgctca aatgcaagct agtaatgctc
    23881 aatttactac cgttttcgga gatatggaaa cccaagcaag agaagcgttg aatgctattg
    23941 gtcaggaaat ggatattgtc ccagagcgat tgcaaggatc attcactcag atggcttcat
    24001 ttgcaaaaac ttcaggattg gatacagcag aagctttgga tcttacttct cgtgcaacta
    24061 gggcagcagc agacggtgca gccttctatg acaaatctat tgagagcgtg acagagagct
    24121 tacaatcttt tttgaaggga aactttgcta acgatgcggc tcttggcatt tctgcaacag
    24181 aaacgaccag gaatgccgct gcaaataaat tgtacggaaa gtcattcaag gacttgagcg
    24241 aagcgcagaa gcaattgacc ttgcttcaga tggtcgaaga cggaaataaa ctctcaggag
    24301 ctcttggaca ggctgcaaga gaatcagacg gcctggaaaa cgtgatgggg aatctgaaac
    24361 aagctgggac caatgcatta tctgctattg gtcaacctct tctagaaatg atgattcctg
    24421 tttttcaaac cttggcaacg attgtgaaag gtgtagctga gctgttcagt tccttacctg
    24481 ctccagtaaa agattttgtt gttattttag gaacagttgt gactgctgta ggggtcatag
    24541 cccccatatt cttatcgttg caagcccttg ctgagttttt aaaaatatct attggagaaa
    24601 tgataattgc cgcattgcca attattggaa cagctattgc aattgctgct gcagttgctg
    24661 caattattgt tattgtaaaa tatctctggg aaactaacga aggttttcga gatgcggtca
    24721 cgaccgtttg gaatgcgatt cttgaagtta tcaatgcagt cgtatcagag atttctaatt
    24781 ttgtcatgag tatctttgga acggttgttg cttggtggac ggagaaccag gaacttattc
    24841 gagcaagtgc tgagactgtc tggaatgcta tctatacggt tataagcaca attctggaaa
    24901 ttttaggtcc actcattcaa gctggttggg ataatatcca acttgtcatt acaacagctt
    24961 gggaaatcat taagactgtt gttgagactg caataaacgt tgtccttggt atcattcaag
    25021 cagttatgca gatcatcaat ggtgattggt caggcgcttg ggaaactatt aagggggtat
    25081 tctctactgt atggcaagct atccaaagca ttgtacagac cattttttca gctatccaga
    25141 gttatatttc aaatattctc aacggcattt caggaactgt atcaaatatc tggaacagca
    25201 tcaaggacac tgtctcaaat gtgttaaatg ctatatctag tactgtatca agtgtttggg
    25261 aaggtatcaa gagtaccatt tcaagtgcta tcaatggtgc aagggatgct gtttcttcag
    25321 ctattgaagc catcaaagga ttgtttaact tcaacatcag ctggccacat atcccactac
    25381 ctcactttta cgtgagtggt tcggccaatc cattagattg gttgagtcaa ggtgttccaa
    25441 gtattggaat cgaatggtat gccaaaggcg gtatcatgac gaaaccaact atctttggaa
    25501 tgaatggtaa taacataatg gttggtggtg aagctgggaa tgaagcagta ttgccactta
    25561 acgacaaaac gcttggagcc atcggtcgag gtatcgctca aactatgggt ggaacttcac
    25621 caaccatcaa cattactatt actggtaaca ccgtcagaga agaagctgac atcagtcgga
    25681 ttgctgatga ggtggctcag cgtattgctg acgaattaca acgtaggaga caattgagag
    25741 gagggtttgc atgataaagc ataatgagct tgtgattgac ggtgtgagga catcgtcttt
    25801 tccatttaag atcattgtcc atgactctcc ttcaatcgct ctaggagaga gcaagacagc
    25861 tctcttggag catggtggtg tcagtggggc aattgttcag acgaacaagc acagggaact
    25921 ggtcaagaaa tcttatacga tttacttagt caaaccgact gaggagcaga tgaaccaatt
    25981 tatgagtctg tttatccgtg agaagttctg gttagagagc gagcgagtca aaacaactcg
    26041 tctctggtgc tataaggtca atgtgactga ccttgaagaa gtacaacctg gtctttacat
    26101 gaccaaagcg accttcactt gccatcctac aaaatacttc aaagcaactg atactcagag
    26161 attgacaaga agcgggactc tgaatgtgca aggttctgct cttgcttttc ctaaaatcac
    26221 aatcgttggt cagagcgttg ttgagacttc atttacaatc gctggtcagg ttattaggct
    26281 tgaaaaactc tcagaatcgc ttgtgatggt taataatcct gacaatccta gctttaaaac
    26341 gacaacaggg aagccagtga aatggtcagg ggattttatc acagttgatc cagcgaaagt
    26401 taagaatatt ggggttgttt taggcccagg tattcaatcg attgaaatcg aaacggtttg
    26461 ggggtgggca taattgcttt atctacttaa taaagatgta agaaccgttc gatggaacgg
    26521 ggagccactt catgaagcga cttcggcgat tgtgaaagaa actatgaatg gcgattttac
    26581 cttgactgtg aaatatccta tttctgattc tggtatttac cagcttatcc aagaagatat
    26641 gctgataaag gctccgactc ctgtcttagg tgcgcagcta tttcgcatta agaaacctgt
    26701 tgagaacaat gaccatctgg aaattacagc ctatcacatt tcagatgatg tgatgcaacg
    26761 ttctataaca ccagtaagtg tgactagtca gagctgtggc atggctcttt ctcgcatggt
    26821 tcaaaacacc aaaaccgctt tgggagattt ttctttcaat agcgatatcc aggaccgtag
    26881 gaccttcaat acgactgaaa tagaaactct gtactctgta ttgctggacg gtaagcacag
    26941 cattgttggt acatgggaag gcgagctggt tcgtgataac tttgcgatga ctgtaaagaa
    27001 gagtcgtggt gagaatcgtg gtgttgttat tacaacacat aagaatctga aggactacca
    27061 acgaaccaaa aacagtcaaa atgttgttac aaggattcat gctcgatcca catttaagcc
    27121 tgaaggtgct gaaaaggaaa caactatcag agtgactgtt gatagtcctc ttattaattc
    27181 ttatccttac ataaacgaaa aagagtatga gaacaacaac gcaaaatccg ttgaagagtt
    27241 gcagaagtgg gcacaggcta agttctcaaa tgagggcatt gacaagatct ctgacgctat
    27301 caagattgaa gcttatgaac ttgatgggca agttgttcac atgggtgata cggtcaatct
    27361 caagagctgg aaacataatg ttgatgtatt taagaaagct attgcttatg agttcgatgc
    27421 cttaaaagaa gaatatatct ctctgattct cgatgataag gcaggagctg gtggttctag
    27481 aacatctggt ggcttatcta gcgcagcgta tgccattctt ggagtaacag aatctgcaca
    27541 agaagttgcc cttgaaaagg ctcttcaaaa tgctgactta gactttgatc ataaggctgg
    27601 attgcttaga caggaaattt ctgacggcat cgaacttgcc aaggccaagg cggaagaagt
    27661 caagcaagaa ctgtctgaca ctatcaatca gcgctttaat agctttgaca acgggccatt
    27721 gaaagaagct aagcgcaagg ctgaggaagc tttgcgaaat gctggcgcaa gtagttctct
    27781 tgctcaggaa tccaagcgga ttgggctgga ttctgttgct agacttgaag cgtttaagtc
    27841 gcagactacg agcgcacaaa cggctctgtc aggtgacttg gacgctctga aacggactat
    27901 cgtgaatgat attcgaccga agcaagcaca ggttgaagct gagattgcca agcaagttga
    27961 agcacttgtt cagaccaaaa aagaactgtc tggcgcaagt accct
//
LOCUS       DBEROU010000029        26708 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.029, whole genome shotgun sequence.
ACCESSION   DBEROU010000029 DBEROU010000000
VERSION     DBEROU010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 26708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26708
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.029"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            58..729
                     /locus_tag="ACL8CL_001342"
     CDS             58..729
                     /locus_tag="ACL8CL_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330584.1"
                     /translation="MIRLPNKFKGNNITLEVKLNKAVDASVLYPDVKSLDQELYRQLQ
                     SVESFYLTFSDMYSEINENNEILYTEIIFKIPLCIGEDYFNFPVVSYVSNPYSLIRGF
                     YLGFHKELDCNYVFDDAELHFFREGICEFNFEFKNQNSIITAELPREYNYPLILFNNS
                     NLVNQYGKYTLLDIDKYKLESRKIFEFPEISIGNFLGSEAKSNRVIFSQDKFQINGIK
                     NMEVN"
     gene            731..1759
                     /gene="galE"
                     /locus_tag="ACL8CL_001343"
     CDS             731..1759
                     /gene="galE"
                     /locus_tag="ACL8CL_001343"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HGS1330585.1"
                     /translation="MKSLLITGGAGYIGSHTVLDLLDNNYEVTIIDDLSNSKKKVIES
                     IKQISKKSRNLHFYKIDLKNEEKLENVFKRHNFDGVIHFSGYKAVGESVVEPLKYYEN
                     NLLSTINILKLMKKYKVFNFVFSSSATVYESTPIMPFYETNPLKASNPYGRTKQYIEV
                     LLNDLFISNSNWKIVCLRYFNPLGAHESGDLGEDPNGIPNNLVPYITQVAIGKLPYLN
                     IFGVDYSTPDGTCIRDYVHVNDLAYGHRKALEYIFNTDEGLYEVINLGSGVGFSVFEI
                     LHSLESVIGSYIPYKITSRRAGDMDVSIADISKAEELLGWKPRYDIMKMCQDTWKWQQ
                     KHPNGYDD"
     gene            1783..3027
                     /locus_tag="ACL8CL_001344"
     CDS             1783..3027
                     /locus_tag="ACL8CL_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit family protein"
                     /protein_id="HGS1330586.1"
                     /translation="MHIVLFRYSARVVKLKYLYNRNVVIIAPEVQRKKYEDYLAEGNN
                     LPKVIFLEDFILPNIILEIRRISQNTEVESITTLSEEDMDIVGFLNDHFVEKNTHSVS
                     NLLFKDKYFMRSFLVGVVNQPYFRLLESENDLRVFWNNCKSSAAILKPRNEAGSNGIK
                     KVYPNEKIDSVYFEKNYIIEEYIDIDNMITCDGYSIGSEIKRFYVHEYEELLFNTLSS
                     SGYYLVRTSSLYNDSEKIEYIEKALEECRKVLNVFSINSEITPFHFEWFLELSTGKIV
                     FCEVGKRFGGGDIPQLIEDSHGVDVIKEYWDILTNSQGLKKINFSKVISRPANISATF
                     ALYRKQGKVIEVPDEKELAWAKKVYIFIKPNTYYKVSENVVENSMLVQFNCKNSCEFE
                     KRLDELKHISQKFKYVITDSGE"
     gene            3029..4240
                     /locus_tag="ACL8CL_001345"
                     /pseudo
     CDS             3029..4240
                     /locus_tag="ACL8CL_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4920..5141
                     /locus_tag="ACL8CL_001346"
     CDS             4920..5141
                     /locus_tag="ACL8CL_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1330587.1"
                     /translation="MYRRLRDLREDHDLTQKQIAKILSFTNSAYAKIERGEHTLTADV
                     LVTLSNFYDVSTDYLLELTDFPDKIRFRK"
     gene            5587..5911
                     /locus_tag="ACL8CL_001347"
                     /pseudo
     CDS             5587..5911
                     /locus_tag="ACL8CL_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            6039..7268
                     /locus_tag="ACL8CL_001348"
     CDS             6039..7268
                     /locus_tag="ACL8CL_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HGS1330588.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGITIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            7288..9984
                     /gene="ppc"
                     /locus_tag="ACL8CL_001349"
     CDS             7288..9984
                     /gene="ppc"
                     /locus_tag="ACL8CL_001349"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HGS1330589.1"
                     /translation="MSLQKLENYSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDCIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    10067..10284
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10397..11401
                     /gene="trpX"
                     /locus_tag="ACL8CL_001350"
     CDS             10397..11401
                     /gene="trpX"
                     /locus_tag="ACL8CL_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1330590.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVATMSKQLVANGND
                     LVVGIATSAAQGLASATKDLPVIMAAITDPIGANLVKGLKKPGGNVTGVSDHNPAQQQ
                     IELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSTGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            11470..12336
                     /locus_tag="ACL8CL_001351"
     CDS             11470..12336
                     /locus_tag="ACL8CL_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1330591.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            12333..13091
                     /locus_tag="ACL8CL_001352"
     CDS             12333..13091
                     /locus_tag="ACL8CL_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330592.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFERDFITILGGN
                     GAGKSTLFNTIAGTLSLTSGTIRILGEDLTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLLPRRLTSYKDEFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRIIQDLNQEEKAKMKISDYYQLFE"
     gene            13181..14941
                     /gene="dnaG"
                     /locus_tag="ACL8CL_001353"
     CDS             13181..14941
                     /gene="dnaG"
                     /locus_tag="ACL8CL_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HGS1330593.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKAGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KSASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKRSSGKASEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKALDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRISPIEFYIHQYKPENSENLQAQIEFLEKIAPLI
                     VQEKSIAAQNSYIHILADSLASFDYTQIEQIVNESRQVQRQNRMEGISRPTPITMPVT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            14944..16053
                     /gene="rpoD"
                     /locus_tag="ACL8CL_001354"
     CDS             14944..16053
                     /gene="rpoD"
                     /locus_tag="ACL8CL_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HGS1330594.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            16067..16396
                     /locus_tag="ACL8CL_001355"
     CDS             16067..16396
                     /locus_tag="ACL8CL_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HGS1330595.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            16511..17554
                     /gene="cpoA"
                     /locus_tag="ACL8CL_001356"
     CDS             16511..17554
                     /gene="cpoA"
                     /locus_tag="ACL8CL_001356"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HGS1330596.1"
                     /translation="MEKKKLRINMLSSSEKVAGQGVSGAYRELVRLLHRAAKDQLIVT
                     ENLPIEADVTHFHTIDFPYYLSTFQKKRSGRKIGYVHFLPATLEGSLKIPFFLKGIVK
                     RYVFSFYNRMEHLVVVNPMFIEDLVAAGIPREKVTYIPNFVNKEKWHPLPQEEVVRLR
                     TDLGLSDNQFIVVGAGQVQKRKGIDDFIRLAEELPQITFIWAGGFSFGGMTDGYEHYK
                     KIMENPPKNLIFPGIVSPERMRELYALADLFLLPSYNELFPMTILEAASCEAPIMLRD
                     LDLYKVILEGNYRATAGREEMKEAILEYQANPAVLKDLKEKAKNISREYSEEHLLQIW
                     LDFYEKQAALGRK"
     gene            17570..18895
                     /locus_tag="ACL8CL_001357"
     CDS             17570..18895
                     /locus_tag="ACL8CL_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS1330597.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLAAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLQQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            18926..19102
                     /locus_tag="ACL8CL_001358"
     CDS             18926..19102
                     /locus_tag="ACL8CL_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGS1330598.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            19161..19295
                     /locus_tag="ACL8CL_001359"
     CDS             19161..19295
                     /locus_tag="ACL8CL_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HGS1330599.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            19359..20669
                     /gene="obgE"
                     /locus_tag="ACL8CL_001360"
     CDS             19359..20669
                     /gene="obgE"
                     /locus_tag="ACL8CL_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HGS1330600.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEDFKKKLAENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            20679..20837
                     /locus_tag="ACL8CL_001361"
     CDS             20679..20837
                     /locus_tag="ACL8CL_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330601.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            21945..23204
                     /locus_tag="ACL8CL_001362"
     CDS             21945..23204
                     /locus_tag="ACL8CL_001362"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HGS1330602.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            23220..23771
                     /locus_tag="ACL8CL_001363"
     CDS             23220..23771
                     /locus_tag="ACL8CL_001363"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1330603.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            23764..25041
                     /gene="spxR"
                     /locus_tag="ACL8CL_001364"
     CDS             23764..25041
                     /gene="spxR"
                     /locus_tag="ACL8CL_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS-HotDog domain-containing transcription
                     factor SpxR"
                     /protein_id="HGS1330604.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATTINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            25057..25917
                     /locus_tag="ACL8CL_001365"
     CDS             25057..25917
                     /locus_tag="ACL8CL_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HGS1330605.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKSGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVIGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            26296..26708
                     /locus_tag="ACL8CL_001366"
                     /pseudo
     CDS             26296..26708
                     /locus_tag="ACL8CL_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         8399 a         4355 c         5761 g         8193 t
ORIGIN      
        1 tgaagagttt gacaaaatag tttctaaaat attaaaaatt aaagtgggag ggtaactatg
       61 ataagattac caaataaatt taaaggtaat aatataactc tagaagttaa actaaataaa
      121 gcagttgatg ctagtgtatt atacccagat gttaagtctc tagatcaaga attgtatcga
      181 caactacaat cagtagaatc attctatctt accttttctg atatgtattc agaaattaat
      241 gaaaataatg aaatcttata tacagaaata atctttaaga tacctctttg tattggagaa
      301 gattatttta attttccggt tgtatcatat gtttcaaatc cctattcgtt aataagagga
      361 ttttatctag gttttcataa ggaattggac tgtaactatg tatttgatga tgcagagctt
      421 cattttttta gggaagggat ttgtgaattt aacttcgaat ttaaaaatca aaattcaatc
      481 attacagcag aattaccaag ggagtataac tatcccttga tcttgtttaa taattccaat
      541 ctggttaatc aatatggaaa atatactttg ttagacatag acaaatataa attagagtct
      601 agaaagattt ttgaatttcc agaaatttct atcggtaact ttcttggaag tgaagcaaaa
      661 tcaaataggg taatttttag ccaagataaa tttcaaatta atggcataaa gaatatggag
      721 gtaaattagc atgaaatcat tattaataac aggtggagca ggatacatag gatcacatac
      781 agttttggac ttgttagata ataattatga agtaacaatc attgatgatt tgagtaactc
      841 taagaaaaaa gttattgagt ctattaagca gatatccaag aagagtagaa atcttcattt
      901 ttataaaatt gatttaaaaa atgaagaaaa attggaaaat gtttttaaga gacataattt
      961 tgatggagta attcattttt cagggtataa agctgttgga gaatccgtgg ttgaaccatt
     1021 gaagtattat gaaaataatt tattatcaac tataaatatt ttaaaactaa tgaaaaaata
     1081 taaggtattt aattttgtgt ttagttcatc agctactgta tacgaaagta ctcctatcat
     1141 gccattctat gagactaatc cattaaaagc ttcaaacccg tatgggagaa caaaacagta
     1201 tatagaagta cttttaaatg atttgtttat ttcgaattca aattggaaaa ttgtttgtct
     1261 gaggtatttt aatccattgg gtgcacatga aagtggtgat ttaggagagg atccaaatgg
     1321 aattccgaat aatcttgttc cttacattac acaagttgca attggaaaat taccatattt
     1381 aaatatattc ggagttgatt acagtactcc tgatgggact tgtataaggg actatgttca
     1441 cgttaacgat ttagcatacg gacacagaaa agcattagag tatattttta atacagatga
     1501 aggtttatat gaagtaatca atttaggaag tggtgttggt tttagtgtat ttgaaatcct
     1561 acacagctta gagagtgtca taggctcgta tattccatat aagataactt caaggagagc
     1621 aggagatatg gacgtttcaa ttgcagatat ttcaaaagca gaagagctac tgggatggaa
     1681 gccaaggtat gatattatga aaatgtgcca agatacgtgg aaatggcaac agaaacatcc
     1741 aaatggatat gatgactaat aattgaaagg attgggatta acatgcatat agtattattt
     1801 agatattctg cacgggttgt taaactaaaa tatctgtata acagaaatgt agtaattatt
     1861 gcacctgagg ttcaaagaaa aaaatatgaa gattatctag ctgaaggaaa taatctacct
     1921 aaggtcattt ttttagaaga ttttattcta ccaaatatca tcttagaaat tagaaggata
     1981 agtcaaaata cagaagttga gagtattaca accttgagtg aggaagacat ggatatagta
     2041 ggttttttga atgatcattt tgtagaaaaa aatactcact ctgtgtctaa tttattattt
     2101 aaagacaaat acttcatgcg ttcattttta gttggagtag taaatcaacc gtattttagg
     2161 cttttagaat cagaaaatga tttacgtgtt ttttggaata attgtaaatc ttctgctgca
     2221 attttaaaac ctagaaatga agcaggaagt aatggaataa aaaaagttta ccctaacgag
     2281 aaaattgatt cagtgtattt tgaaaaaaac tatattatag aggaatacat tgatatcgat
     2341 aacatgataa catgtgatgg ttattcaatt ggaagtgaga ttaagcgatt ttatgttcac
     2401 gaatatgagg aactattgtt caatacctta agttctagtg gatattatct tgtaaggact
     2461 agttcgttat ataatgatag tgaaaaaatt gaatacatag aaaaagcatt ggaagaatgt
     2521 cgcaaagttt tgaatgtttt ttctatcaat tctgaaataa ccccttttca ttttgaatgg
     2581 ttccttgaat tatcaactgg taaaatagtc ttttgtgaag ttggaaaaag atttggaggg
     2641 ggagatattc cccagttaat tgaggatagt catggagttg atgtaattaa agaatattgg
     2701 gatattttga ctaattcaca aggattgaaa aaaataaatt tttctaaagt gatttctaga
     2761 cctgctaata tctcagcaac gtttgcttta tatagaaagc aaggtaaggt tatcgaggta
     2821 cctgatgaaa aagaattggc ttgggcaaaa aaagtgtaca tatttattaa accaaatact
     2881 tactataaag tatctgaaaa tgtagtcgaa aattcaatgt tggtccaatt taattgcaaa
     2941 aatagttgtg aatttgagaa aaggttagat gaacttaagc acatatcaca aaagtttaaa
     3001 tatgtgatta ctgattcggg agaataaaat gattagagaa gaacatcaca atattctatc
     3061 cattgtagct agtaggatga tatctcgaat cggagatatc atgtttgatt tcgccaataa
     3121 tacctttctt gctggtctta atccaacatc tctatcagtg gttgcaattt atcaatcatt
     3181 agaaagtatg ataggtgttc tatttaattt atttggtgga gttattgcag atagtttcaa
     3241 gcggaaaaaa attattatta ctacaaatat cttatgtggt attgcttgca taactctttc
     3301 atttatatcc caagaaaaat ggttggttta tgcgatagtc ataacaaatg tcattttggc
     3361 ttttatgagt gctttttcag ggccatcata caaagctttt acgaaagaaa ttgtaaaaaa
     3421 ggacagtata tcataactta attcattgct agaaacaaca agtactgtga ttaaagtaac
     3481 aattcctatg attgcaattt tcttatataa gttacttgga atacatggtg tgttattact
     3541 agatggatta tcgtttttaa ttgctgcctc actaattttc ttcattactc ctgtaaatga
     3601 ggaggtggat acaaaagaga atatgacgat aagaggaatc tttgatgatt taaaaatagg
     3661 atttacgtat gtatatagtc ataaacaaat tctcactata attgcccttt cagcacttgt
     3721 taactttttc ctagcagcct ataatttgtt gctgccatac agtaatcaaa tgtttggaag
     3781 tatttcaagt gggctatatg gaaccttttt aacagccgaa gcgattggtg gctttattgg
     3841 tgcgatatta agtggattta tcaataaatc cttgtcaagc aaacgtttaa tgctcttttt
     3901 agcatattca ggtttgatgt taatgctaac agcgccgatc tattatatgt tccgcaacgt
     3961 cattatttta gcctttactc cagcattatt tagtctattt ctttccattt ttaatatcca
     4021 atttttctct attgttcaaa gagacgtaga taatgagttt cttggcagag tatttggaat
     4081 catctttacg gtagctattc tttttatgcc actaggtact gtttttttct cagtagtttt
     4141 aaatcctaac aatactttta atttttttat tattggtgta tctattacga tattatcgct
     4201 aatatttagc acgctattga agaggtatga taaaagttga tagatgataa aaaatagctg
     4261 atatagagaa tttagagcgc ctttggaata tatttttgga atttaatcaa tcacagttga
     4321 atgtgaaggg caatcctttt tagaagaata caatcaagat accattatgc tatttcttta
     4381 gttcagttaa aatattgaga agtattactt ttattgaaaa agcatcacaa aggactccaa
     4441 ttaattttta atgatttttc taatctaatt ttgtggttat tgacaaataa agtctagtta
     4501 attgctttga aatccatgga gtggtctata tacatctgtt aatgcaacgg aaaattttat
     4561 tgataagtag ggggtggcta ttattcaact tttaggttct gaaagtaaga gattcacatt
     4621 agaaatatca tgttgaccat ttttcaattt gctggttagt agtaatctgg acatcattaa
     4681 tcccttcctt aacaaaatta tgtgactttt gttaccggat tactgctgat tttttaatag
     4741 tttataacat ctttagacgg ttaatagatt ttgaccgtct ttcttaatat tgtcttacct
     4801 ataatagata tgcttatgag atatgtttaa taaattaatt ttagttcaaa atgtccagtt
     4861 tggacacttt gaatgatttc ttagttttat atattatact tttctcagga gaaagctaga
     4921 tgtacagacg tttgagagat ttgagggaag atcacgattt gacccaaaaa caaatagcta
     4981 aaatactttc gtttacaaac tcagcttatg ctaaaattga acggggtgag catacgttaa
     5041 cagcagatgt attggtaact ctctcaaact tttacgatgt cagtacagac tacctattgg
     5101 aattgacaga ttttcctgat aaaattcgct ttagaaaata atctcctcaa tttcatagag
     5161 tgtgaaaatg aggagatttt ttatttgttc tttgaaaatt aaatagtcca aaatggtact
     5221 ttcctcattt gtggagcaaa tttgaatggc tcgccatgat aagagcgatt ttaaaaccat
     5281 caataaaata gagcgatact ttatatgcca tgatacaaat gatatacaat gatacttctg
     5341 accgttcagg ctgccatcgt aaaagagcag caagtgaaat tcttatgatg acttcatcag
     5401 tcatgccacg ttaaatgtgt gagtttgtta gagaaacgca attaatcctc aaaaggttcc
     5461 ccgaaccttt tgagttccac agacgcatca cgtggagtgt gtaagcttgt tgctaaaagc
     5521 gtaaaaacct tggaacgaaa ggaataatag acttcctgcg aaacaaaaat acaatacaat
     5581 aaaactatgg atgatgaagc aagtaaacaa ttaagcgata gccgtttcaa gagccttgta
     5641 ggtgttcagc gcacgagatg ttagctgtgt caaaaacagc ttatcaacgc aacgtaaacg
     5701 cgcaaaaggt ggacgaaaaa ccaaattaag cctagacgat ctcctcatgg taactattca
     5761 atacatgcga gaatagcgca cttatgaaca aattgcggct gattttggca ttcacgaaag
     5821 caacttaatc cgtcggagcc aatgggttga agtaactctt gttcaaaatg gttttacgat
     5881 ttcaaattct gccctaatta tgaaaaacag taaaattcga aggattgtaa ggtaagagtt
     5941 tttttctttc tgaaaaaatg gtataatagc aatcaaaact agaaaataaa acggaatttg
     6001 gaacagattt gtctgtatcc tagtagagtg gtgatgctat gaagattagt aagaggcact
     6061 tattaaatta ttccatcttg attccctact tgcttttatc tattttgggc ttgattgtgg
     6121 tctattcgac caccagtgct attttaattg aagaaggcaa gagcgccttg cagttggttc
     6181 gaaaccaagg aatcttttgg attgttagtt tgatactgat tgccttaatt tataaattga
     6241 gactagattt tttgagaaat gagcgactaa tcattttagt tatattaata gaaatgcttt
     6301 tattgttctt ggctcgtttt attggtattt ccgtaaacgg ggcatacggt tggatttcgg
     6361 ttgcaggaat aactattcag ccagctgagt acttaaaaat cattattatt tggtatttag
     6421 ctcaccgatt ctccaaacag caagaagaaa tagctactta tgattttcaa gttttgactc
     6481 aaaatcaatg gcttccccgt gcttttaatg attggcgatt cgttctccta gttctgattg
     6541 gaagtttggg aattttccct gatttaggaa atgcgactat tttagtcttg gtttccttga
     6601 ttatgtatac agttagtgga atcgcttatc gctggttttc aaccattctg gcgctcgtat
     6661 ctgccgcttc tgtctttgtc ttgaccacta tcagcctaat cggtgttgag accttttcaa
     6721 aaattccagt atttggctat gtagccaagc gctttagtgc cttttttaat ccttttgccg
     6781 atcgtgctga tgcaggtcac cagttagcta attcttattt tgccatggtc aatggcggtt
     6841 ggtttggtct aggtcttgga aactcgattg aaaaacgagg ttatttgcca gaagctcata
     6901 cagactttgt cttttctatc gtgattgaag aatttggctt tgttggtgcc agtcttattt
     6961 tagctctctt gtttttcatg attttgcgga ttatcttggt cggtattcga gcggagaatc
     7021 ctttcaatgc catggttgca ctcggtgtcg gagggatgat gttggttcag gtatttgtca
     7081 atatcggagg gatttcgggc ttgattccat ctacaggagt aaccttcccc ttcttatccc
     7141 agggtggaaa tagtcttcta gtcttatcag tggcagtagc ctttgtctta aatattgatg
     7201 ccagtgaaaa acgcgctaag ttgtaccgag aattggaaaa tcaaccaatg aaccttctgt
     7261 tgaagtagga taaagaaagg atagtttatg tctcttcaaa aattagaaaa ttatagtaat
     7321 aaaagtgttg tgcaagaaga agtcttgatt ctaacagaat tactggaaga tattactaaa
     7381 aatatgcttg ccccagagac ctttgaaaaa ataatacagt tgaaagaatt atcaacgcag
     7441 gaagattatc aaggtctaaa ccgtctagtg actagcttat caaatgatga aatggtctat
     7501 atttcacgct atttctctat cttgcctctt ttgattaata tttcagagga tgtggattta
     7561 gcttatgaaa tcaatcatca aaataatatt gatcaggact atttaggtaa attatctaca
     7621 acgattaaat tggtagcaga aaaggaaaat gccgttgaga tcctagaaca cttgaatgtt
     7681 gtccctgttt tgacagccca tccaacacaa gtgcaacgca aaagtatgtt ggatttaaca
     7741 aatcatattc atagtctttt gcgtaaatac cgtgatgtta agttggggtt gatcaataaa
     7801 gataaatggt acaatgattt gcgtcgttac atcgaaatta tcatgcagac agacatgatt
     7861 cgtgagaaaa aattaaaagt gactaacgaa atcacgaatg ctatggaata ttataacagc
     7921 tcctttttga aagctgtacc tcatttgacg acggagtata agcgcttagc gcaagcgcat
     7981 ggtctgaatt taaaacaggc taaaccaatc accatgggta tgtggatagg tggtgaccgt
     8041 gatggaaatc catttgttac agcaaagacc ttgaagcagt ctgcactcac tcagtgtgaa
     8101 gtcatcatga actactatga taaaaagatt taccaacttt atcgtgaatt ttctctttca
     8161 actagcattg tcaacgtcag caagcaagtc agagaaatgg ctcgtcaatc caaggataac
     8221 tcgatttacc gcgaaaaaga gctttaccgt cgtgccttgt ttgatattca atcaaaaatt
     8281 caggcaacta aaacctatct gattgaggat gaagaagttg ggactcgtta tgaaaccgcc
     8341 aatgatttct acaaggattt gattgccatt cgagattctc tactagaaaa taagggtgag
     8401 tccttgattt caggtgattt tgtggaatta ttgcaggcag tagagatatt tggtttttac
     8461 ttagcgtcaa ttgatatgcg acaagactct agcgtctatg aagcctgtgt ggcagaactc
     8521 ttgaaatcag caggaattca ttctcgttat agcgagttga gcgaagaaga aaagtgtgac
     8581 cttctcttga aagaattaga agaagatccc cgaattcttt ctgcgactca cgcagaaaaa
     8641 tcagaattat tagcaaaaga attagctatt tttaagacgg ctcgtgtttt gaaagataag
     8701 ttgggagatg atgtcatccg tcagaccatc atttcacatg caaccagcct ttctgatatg
     8761 ctagaattag ctattctgtt aaaagaggta ggactggtgg atacggaaag ggcgcgtgtt
     8821 cagattgttc ccctttttga aacaattgaa gacttggatc attcagagga aacaatgaga
     8881 aaatatcttt ctcttagcct tgccaaaaaa tggattgact cacgaaataa ctaccaagaa
     8941 atcatgcttg gctactctga cagtaataaa gatggcggtt acttgtcatc atgttggacc
     9001 ctctacaagg ctcaacaaca attgactgct attggagatg aatttggcgt taaggttacc
     9061 ttcttccatg gtcgtggtgg tactgtcggt cgtggtggtg ggccaaccta tgaagccatt
     9121 acatctcaac cgctcaagtc tatcaaggat tgtatccgct tgacggagca gggtgaagta
     9181 attgggaata aatacggtaa caaagacgcc gcttactata accttgaaat gctagtatcg
     9241 gcagctatta accgtatgat tactcagaag aagagcgata ccaatacccc aaatcgttat
     9301 gaagccatta tggatcaagt agtggaccgt agttacgata tctaccgtga tttggtcttt
     9361 ggtaatgagc atttctatga ttatttcttc gagtcaagtc caatcaaggc tatttcaagt
     9421 tttaatattg gttctcgtcc agccgctcgt aagactatta ctgaaatcgg tggtttgcgt
     9481 gccatccctt gggtattctc atggtcacag agtcgtgtta tgttccctgg atggtacggg
     9541 gttggttcaa gcttcaagga atttatcaat aaaaatccag agaatattgc tatcttacga
     9601 gatatgtacc aaaattggcc tttcttccaa tcgcttcttt caaatgttga tatggttttg
     9661 tcaaaatcaa atatgaatat tgcttttgaa tatgctaaac tttgtgaaga cgagcaagtt
     9721 aaggccatct atgagactat tttaaatgaa tggcaagtta ctaagaacgt tatcttggct
     9781 attgaaggac atgacgaact cttagctgac aatccatatc taaaagctag tctggattac
     9841 cgtatgcctt actttaatat tctcaactat attcagttag agttgattaa acgccaacgt
     9901 cgtggagaat tgtccagtga tcaagaacga ttgattcata tcaccatcaa cggaattgcg
     9961 acaggattgc gtaattcagg ttgataattt tcaagagtga atgctaaaag tgaatatcaa
    10021 aaaaattcta atagactatt gacaagtagt ttaaaaatga tataatttaa ccattcagaa
    10081 aagtaatcat acaaactttt tagagagtct gtggtagctg aaaacagata agtggcgatg
    10141 atgaaaattg ggctgaatgc tatttagaat ttgaaattat aaaaattcgg taagcacacc
    10201 ttacagtgca tctcgttatt gcgagactga gcgataggga aattccctat aattgaggtg
    10261 gtaccgcgca tcgacgtcct tacacaagtt ttttgtgtga ggattttttt gatggaggtt
    10321 agtatggaaa gaaaacgatg gcgtcgcttg tttagataag tgaaatatgt taaaggaaat
    10381 aaaaaggaga aacagaatga aaaataaacg tttaattgga attattgctg cattagcagt
    10441 cttagtagca ggaagcttga tttattcttc aatgaataaa tcagaagctc agaataataa
    10501 ggatgagaag aaaataacca agattggtgt gcttcaattt gtgagccatc catcccttga
    10561 tttgatttat aaagggatcc aagatggact tgcagaagaa ggatataaag atgatcaagt
    10621 taaaattgat tttatgaact cagaaggtga ccaaagtaag gttgcgacaa tgagtaaaca
    10681 attggttgca aatgggaatg accttgtggt tggtatcgca acatcagcag cccaagggtt
    10741 ggctagtgca acaaaagacc taccagttat catggccgct attacagacc caattggtgc
    10801 taacttggtt aaaggtttga aaaaaccagg tggcaacgtt acaggggtat ctgaccacaa
    10861 tccagctcaa caacaaattg aactcatcaa ggctctgaca ccgaatgtga aaacaatcgg
    10921 agctctttac tcaagtagcg aagacaattc aaaaacacag gtcgaagaat ttaaggctta
    10981 tgctgaaaaa gcaggtctga cagtggaaac atttgcagtt ccttcaacaa atgaaattgc
    11041 ctcaactgtc actgttatga ctagcaaggt agatgctatt tgggttccaa ttgataacac
    11101 cattgcatca ggatttccaa cggttgtctc tagcaatcaa agttctaaga aaccaattta
    11161 tcccagtgcg acagctatgg tagaagtagg tggtttggca tcagttgtaa ttgaccaaca
    11221 tgaccttggt gtggcaacag gtaaaatgat tgtgcaagtc ttgaaaggtg caaaaccagc
    11281 cgatacccca gtcaatgtct tttcaactgg taagtcagtc atcaataaaa aaatagcaca
    11341 agaactaggt attactattc ctgagtctgt tctcaaagaa gcaggacaag tcatcgaata
    11401 aataaggtag ggaggaggag actcttccca ttttttaaaa agatgaatat agaaaggatt
    11461 aaataaaata tgattgtttc cattatttct caaggatttg tctgggctat tctaggtctg
    11521 ggaatcttta tgacatttag gattttaaac tttccagata tgacgacaga aggttccttc
    11581 cctcttgggg gagctgttgc tgtcactttg ataaccaaag gcgtgaaccc atttttagcg
    11641 acacttgttg ctgtaggagc aggttgtttg gctggaatgg cagcaggcct tctttataca
    11701 aaagggaaga tcccaacctt gctctcaggg attttggtga tgacttcttg tcactcaatc
    11761 atgctcttga ttatgggacg tgcgaattta ggcctgcttg gaaccaagca aattcaggat
    11821 gttttgcctt ttgattcgga tttgaatcaa ctcttgacag gtctcatctt tgtgagtatt
    11881 gttattgctc tcatgctctt tttcttggac actaaactcg gacaagccta tattgctaca
    11941 ggggataatc ctgatatggc tagaagtttc gggattcata ctggacgcat ggagctcatg
    12001 ggcttggtct tatcaaatgg tgtgattgcc cttgcaggtg ccctcattgc tcagcaagaa
    12061 ggttatgccg atgtgtctcg agggatcggg gttatcgttg tggggcttgc aagtttgatt
    12121 attggagaag ttattttcaa gagtttgagc ttggcagagc gtttggttac tatcgttgta
    12181 ggttctatcg cttatcaatt tttagtgtgg gcagttatcg cacttggctt taatacaagt
    12241 taccttcgtt tatacagtgc cttgatttta gcagtctgcc tcatgattcc aacatttaag
    12301 caaacaatct tgaaaggagc caagttaagc aaatgacagc aattgtagaa ttaaaaaatg
    12361 caaccaaaat cgttaaaaat ggctttgatg aagaaaagat tattttaaat gatgtttcct
    12421 tagaaatttt tgaacgggac tttatcacga ttttgggcgg aaatggtgct ggaaaatcaa
    12481 ctctctttaa cactatagca gggaccttat cactaactag tggaactatc cgtattttag
    12541 gtgaagatct cactaagttt tcacccgaga agcgtgccaa gtacctgtct cgtgtcttcc
    12601 aagatccaaa gatggggaca gctccccgta tgacggtcgc tgaaaatctt ttaatcgcca
    12661 agtttcgtgg tgaaaagcgt ggattgttac cacgacgctt gactagctat aaggatgaat
    12721 ttcaggcaac cattgaaaaa gtaggaaatg gtcttgagaa acacttgaat acaccgattg
    12781 agttcttatc aggtggacaa agacaggctt tgagtctctt gatggcaacc ttgaagcgac
    12841 ctgaattact cctgttagat gagcatactg ctgccctgga tccaaagact agtgttgctt
    12901 tgatggaatt gacagatgaa tttgttaaga aagatcagct aacagccctt atgattactc
    12961 atcatatgga agatgctctc aaatacggca atcgcttgat tgtcatgaaa gaaggacgaa
    13021 ttatccaaga tttaaaccaa gaagaaaaag caaaaatgaa aatctctgat tattatcaac
    13081 tctttgaata aagtagggaa aatgagtgaa atggtttact ttttttctga aataaagtat
    13141 actatataaa gtaaactatg ataacatgga ggtattgtgt atggttgaca aacaagtcat
    13201 tgaagaaatc aaaaacaatg ccaacattgt ggaagtcata ggagatgtga tttctttaca
    13261 aaaggcagga cggaactatc tagggctctg tccttttcat ggtgaaaaaa caccatcttt
    13321 caatgttgta gaggacaagc agttttatca ctgtttcggt tgtggtcgct caggtgatgt
    13381 ctttaagttc atcgaggagt accaaggggt tccctttata gaagcagtcc aaatcttagg
    13441 tcagcgtgtt ggaattgagg ttgaaaaacc gctttatagt gagcagaagt cagcctcacc
    13501 tcaccaagct ctttatgata tgcacgaaga tgcagccaaa ttttaccatg ctattctcat
    13561 gacaacgact atgggcgaag aggccagaaa ttacctttat cagcgggggt tgacagatga
    13621 agtgcttaaa catttttgga ttggtttagc acctccagaa cgaaactatc tctatcaacg
    13681 tttgtctgat cagtatcgtg aagaggattt actggattca ggcctgtttt atctctcgga
    13741 tgccaatcaa tttgtagaca cctttcacaa tcgcattatg tttcccctga caaatgacca
    13801 aggaaaggtc attgccttct caggtcgtat ctggcaaaaa acggattcac aaacttctaa
    13861 gtataaaaac agccgatcga ctgcaatttt taacaaaagt tacgaattat atcatatgga
    13921 tagggcaaaa agatcttctg gaaaagctag tgagatttac ctgatggaag gattcatgga
    13981 tgttattgca gcctatcggg ctggaatcga aaatgctgtg gcgtcgatgg gaacagcctt
    14041 gagtcgagag catgttgagc atctgaaaag gttaaccaag aaattggttc ttgtttacga
    14101 tggagataag gctgggcaag ccgcgacatt gaaagcattg gatgaaattg gtgatatgcc
    14161 tgtgcaaatc gtcagcatgc ctgataactt ggatcctgat gagtatctac aaaaaaatgg
    14221 tccagaagac ttggcctatc tattaacgaa aactcgtatt agtccgattg agttctacat
    14281 tcatcagtac aaacctgaaa acagtgaaaa tctgcaggct cagattgagt ttcttgaaaa
    14341 aatagctccc ttgattgttc aagaaaagtc catcgctgct caaaacagct atattcatat
    14401 tttagctgac agtctggcgt cctttgatta tacccagatt gagcagattg ttaatgagag
    14461 tcgtcaggtg caaaggcaga atcgcatgga aggaatttcc agaccgacgc caatcaccat
    14521 gcctgtcacc aagcagttat cggctattat gagggcagaa gcccatctac tctatcggat
    14581 gatggaatcc cctcttgttt tgaacgatta ccgtttgcga gaagactttg catttgctac
    14641 acctgaattt caggtcttat atgacttgct tggccagtat ggaaatcttc ctccagaagt
    14701 tttagcagag cagacagagg aagttgaaag agcttggtac caagttttag ctcaggattt
    14761 gcctgctgag atatcgccgc aggaacttag tgaagtagag atgactcgaa acaaggctct
    14821 cttgaatcag gacaatatga gaatcaaaaa gaaggtgcag gaagctagcc atgtaggaga
    14881 tacagataca gccctagaag aattggaacg tttaatttcc caaaagagaa gaatggagta
    14941 ataatggcaa caaaacaaaa agaagtaaca acatttgacg tacaggtagc agaatttatc
    15001 cgtaatcata agcaaaaagg gacagcaaca gatgatgaaa tcaatgcaag tctggttatt
    15061 ccttttacct tggacgctga tgggattgaa gatctcttgc aacggattca ggatgcaggc
    15121 atttctatca cagataacga aggaaatcca agtgcgcgtg ttctcagcaa tgaagaagaa
    15181 ccagaactca gcgatgagga cttgattggg tcaacttctg ctaaggtcaa tgaccctgtc
    15241 cgtatgtact tgaaagaaat aggggtcgtt cctctcttga ccaatgaaga ggagaaagag
    15301 ttggcactgg ctgttgaagc tggtgatatc gaagccaaac aacgtcttgc ggaagccaat
    15361 cttcgtttgg ttgtttccat tgccaaacgc tatgtcggtc gtggtatgca gttccttgac
    15421 ttgattcaag aaggaaatat gggcttgatg aaggcggttg acaagtttga ctattctaaa
    15481 gggttcaagt tttcaactta tgcaacttgg tggattcgtc aggctatcac tcgtgctatt
    15541 gcggaccaag ctcgtaccat ccgtatccca gttcacatgg ttgaaactat caataaattg
    15601 gttcgtgaac agcggaatct ccttcaagaa ttggggcaag atccgacacc agaacagatt
    15661 gctgaacgaa tggatatgac acctgataag gttcgtgaaa tcttgaagat tgcccaagaa
    15721 ccagtatctc ttgaaactcc tatcggtgaa gaggacgata gccaccttgg agactttatc
    15781 gaagatgaag tgattgaaaa tccagtggat tatacgactc gtatcgtctt gcgtgagcaa
    15841 ttggatgaaa tcttagatac tcttacagac cgtgaagaaa atgttctgcg tctacgtttt
    15901 ggactagatg atggaaaaat gcgcacactt gaagatgtgg ggaaagtctt taacgtaact
    15961 cgtgagcgta tccgtcagat tgaagcaaag gctttgagaa aactacgcca accaagtcgt
    16021 agcaaaccgc ttcgtgattt tattgaagac taagagtgag gaaaatatgg cttatacaga
    16081 agagcaaatt gaaaacatca aaacacggat tttaacagcc ttggaagaag tcatcgaccc
    16141 tgagttggga atcgatattg tcaatcttgg tttgatctat gagattcgtt ttgacggtga
    16201 cacagggcaa acagagattg acatgacttt gacaactatg ggttgtccct tagcagacct
    16261 tttgacagac cagatttatg atgctatgat agaggttcca gaagtaacgg atactgaggt
    16321 taaattagtc tggtatccag cctggacagt tgaaaaaatg agtcgctatg cccgcattgc
    16381 cctaggcatt aagtaaacat ataaaagcat gtgagagact gttggaaaag cgaggaaatt
    16441 tcccctcttt tcctctagtc tctcctttct tttgctgatt ttattcaaag aaaatgatat
    16501 aatagtagtt atggagaaaa agaaattacg catcaatatg ttgagttcaa gtgagaaagt
    16561 agcaggacag ggagtttcag gtgcttaccg tgaattagtt cgtcttcttc accgtgctgc
    16621 caaggaccaa ttgattgtta cagaaaatct tccaatcgag gcagatgtga ctcactttca
    16681 tacgattgat tttccctatt atttatcaac cttccaaaag aaacgctcag ggagaaagat
    16741 tggctatgtg catttcttgc cagctacact tgagggaagt ttgaaaattc catttttctt
    16801 aaagggaatt gtgaaacgct atgtattttc tttttacaac cggatggagc acttggttgt
    16861 ggtcaatcct atgtttattg aggatttggt agcagctggt attccacgtg aaaaagtgac
    16921 ctatattcct aactttgtca acaaggaaaa atggcatcct ctaccacaag aagaggtagt
    16981 cagactgcgc acagatcttg gtcttagtga caatcagttt atcgtagtag gtgctgggca
    17041 agttcagaaa cgtaaaggga ttgatgactt tatccgtctg gctgaggaat tgcctcagat
    17101 tacctttatc tgggctggtg gcttctcttt tggtggtatg acagatggtt atgaacacta
    17161 taagaaaatt atggaaaatc cccctaaaaa tttgattttt ccaggcattg tatcgccaga
    17221 gcggatgcgc gaattgtatg ctctagcgga tcttttcttg ttgcctagtt acaatgagct
    17281 ctttcctatg actattttag aagctgcgag ttgtgaggct cctattatgt tgcgtgattt
    17341 agatctctat aaggtgattt tggagggaaa ttatcgggcg acagcgggta gagaagagat
    17401 gaaagaggct attttggaat atcaagcaaa tcctgctgtc ttaaaagatc tcaaagaaaa
    17461 ggctaagaat atttccagag agtattctga agagcatctg ttacaaatct ggttggactt
    17521 ttatgagaaa caagccgctt tagggagaaa gtaaaaagtg aggtaatcta tgcgaattgg
    17581 tttatttaca gatacctatt ttcctcaggt ttctggtgtt gcgaccagta ttcgaacctt
    17641 gaaaacagaa cttgaaaagc agggacatgc tgtttttatc tttacgacga cagataagga
    17701 tgtcaatcgc tacgaagatt ggcaaattat ccgcattcca agtgttcctt tctttgcttt
    17761 taaggatcgt cgctttgcct accgaggttt tagcaaggca cttgaaattg ctaaacagta
    17821 tcagctagat attatccata ctcagacaga attttctctt ggcctgttgg ggatttggat
    17881 tgcgcgtgaa ttgaaaattc cagtcatcca tacctatcac acccagtatg aagactatgt
    17941 ccattatatt gctaagggga tgttgatccg gccgagtatg gtcaagtatc tggttagagg
    18001 tttcctgcat gatgtggatg gggttatttg ccctagtgag attgtccgtg acttgctatc
    18061 tgattataag gtcaaggttg aaaaacgggt cattcctact gggattgaat tagccaagtt
    18121 tgagcgtccg gaaatcaagc aggaaaattt gaaagaactg cgtagtaaac tagggattca
    18181 agatggtgaa aagacgttgc ttagtctttc gagaatctcc tatgaaaaaa atattcaagc
    18241 agttttagca gcctttgctg atgttctgaa agaggaagac aaggttaaac tggtagtagc
    18301 tggggatggc ccttatctga atgacctcaa agagcaagcc cagaacctag agattcaaga
    18361 ctcagtcatc tttacaggga tgattgctcc tagtgagacg gctctttact ataaagcggc
    18421 ggatttcttc atttcggcat cgacaagcga aacgcaaggt ttgacctact tggaaagctt
    18481 agccagtgga acacctgtca ttgctcacgg aaatccttat ttgaacaacc tcatcagtga
    18541 taaaatgttt ggaaccttgt actatggaga acatgatttg gctggtgcta ttttggaagc
    18601 cctgattgca acaccagaca tgaacgagca taccttatca gagaaattgt atgagatttc
    18661 agctgagaac tttgggaaac gagtgcatga gttttatctg gatgccatta tttcaaataa
    18721 cttccagaaa gatttggcta aagatgatac ggtcagtcag cgtatcttta agacagtttt
    18781 gtatcttcag caacaggtgg ttgctgtacc tgtaaaagga tctagacgca tgttgaaggc
    18841 ttcaaaaaca cagttgatca gtatgagaga ctattggaaa gaccatgaag aatagaaaga
    18901 ggaacagcta tgaaaaaaac aattaatgag aagcggtcgt gataaaaaga ttgcgggtgt
    18961 ttgtgctggg gtggcccatt atctggatat ggatccgact atcgttcaag tcatttgggg
    19021 tgttcttact tgctgttacg gagctggaat tgtagcttac attattttat ggattatcgc
    19081 accagtagca actgactatt gaaaactagc tcaattcatg ctaagataat agaaaataga
    19141 atgtaacgaa ggagagaaaa atggcatttg gagataatgg aaatcgtaaa aaaactatgt
    19201 ttgagaaaat aaccttgttt atcgtgatta tcatgctagt agcaagttta ttgggaattt
    19261 ttgcaactgc aattggtgcc ctcagtaatc tataaaatag attcaagaaa atttagtgac
    19321 tgggatttcc cagccctttt ttaaagtgag aagaaataat gagtatgttt ttagatacag
    19381 ctaagattaa ggtcaaggct ggtaatggtg gcgatggtat ggttgccttt cgtcgtgaaa
    19441 aatatgtccc taatggaggc ccttggggtg gtgatggtgg tcgtggaggc aatgtggtct
    19501 tcgttgtaga cgaaggacta cgtaccttga tggatttccg ctacaatcgt catttcaagg
    19561 ctgattctgg tgaaaaaggg atgaccaaag ggatgcatgg tcgtggtgct gaggacctta
    19621 gagttcgagt accacaaggt acgactgttc gtgatgcgga gactggcaag gttttaacag
    19681 atttgattga acatgggcaa gaatttatcg ttgcccacgg tggtcgtggt ggacgtggaa
    19741 atattcgttt cgcgacacca aaaaatcctg caccggaaat ctctgaaaat ggagaaccag
    19801 gtcaggaacg tgagttacaa ttggaactaa aaatcttggc agatgtcggt ttagtaggat
    19861 tcccatctgt agggaagtca acacttttaa gtgttattac ctcagctaag cctaaaattg
    19921 gtgcctacca ctttaccact attgtaccaa atttaggtat ggttcgcacc caatcaggtg
    19981 aatcctttgc agtagccgac ttgccaggtt tgattgaagg ggctagtcaa ggtgttggtt
    20041 tgggaactca gttcctccgt cacatcgagc gtacacgtgt tatccttcac atcattgata
    20101 tgtcagctag cgagggccgt gatccatatg aggactacct agctatcaat aaagagctgg
    20161 agtcttacaa tcttcgcctc atggagcgtc cacagattat tgtagctaat aagatggaca
    20221 tgcctgagag tcaggaaaat cttgaagact ttaagaaaaa attggctgaa aattatgatg
    20281 aatttgaaga gttaccagct atcttcccaa tttctggatt gaccaagcaa ggtctggcaa
    20341 cacttttaga tgctacagct gaattgttag acaagacacc agaatttttg ctctacgacg
    20401 agtccgatat ggaagaagaa gcttactatg gatttgacga agaagaaaaa gcctttgaaa
    20461 ttagtcgtga tgacgatgcg acatgggtac tttctggtga aaaactcatg aaactcttta
    20521 atatgaccaa ctttgatcgt gatgaatctg tcatgaaatt tgcccgtcag cttcgtggta
    20581 tgggggttga tgaagccctt cgtgcgcgtg gagctaaaga tggggatttg gtccgcattg
    20641 gtaaatttga gtttgaattt gtagactagg agactggtat gggagataaa ccgatatctt
    20701 tccgagatgc ggatggtaat tttgtttccg ccgcagacgt ttggaatgaa aagaaattgg
    20761 aagaactatt taatcgtctc aatccaaatc gtgccttgag attggcacga actaaaaagg
    20821 aaaatccatc tcagtaaaga agctaaaaaa tcccgtgcct catcagacac gggattttgt
    20881 ggtacgacag gcatgtatag caaactgaat ctggaatagc acagcatatc ttctaaaata
    20941 tagtaaaatg aaatgagaac aggacaaatc gatcaggaca gtaaaatcga tttctaacaa
    21001 tgttttagaa gcagagatgt actattctag tttcaatcaa ctatattgtt ataaattgat
    21061 ttgaatttca aaattaaatt gtttgattct tatttcaatt tgttatagta tatctgatgt
    21121 caaagttctc ggcgagtcaa atagcgattc ccaagcctga ctatcgtgag gtagcggatt
    21181 aaaatggtct ggggatagac cgttttaagt ctgacgctgg aaataagaat tgtcagaaga
    21241 agggatagcg aaatcgtggc tctacgaaca ggaacgtgat aataaggcgt atatagcgga
    21301 taagagggcc tcaaactcta aagtccaaaa aggtagtcgt aacctatatg cgtaaatcac
    21361 gagagtaatt gaattcgtac taagattttc tattttcact gtaacctttt aacgtcctcg
    21421 tatcttgtat acacgaggaa agatgtacga cttatcccgt gaggtctatc actataaaga
    21481 gaaaacgaca gatagaagtg atcctgagtc acggttatct ttaaaataga ttgtctgata
    21541 ggacggtatg tataaaacgc ttctgtgaac tgagagaagg gggagaagtt cttgctaaaa
    21601 tttagttgaa cagccgtatt ccgatattta gataagggat ctagtcttag ctcctactca
    21661 gttttagggg ataaaaaagg ggcaatagcg attcgagaaa gattatactc ttcgaaaatc
    21721 tcttcaaatc acgtcaatat cgccttgccg tatgtgtagg atactgacta cgtcagttct
    21781 atctacaacc tcaaaacagt gttttgagca acctgcggct agtttcctag tttgatcttt
    21841 gattttcatt taaaattgaa aaggatggaa aaaaggataa atttatgata tactttattt
    21901 tgaagacctt attagaaatc ttgaaagagt attgaaaact tagaatgaga aaaattgtta
    21961 tcaatggtgg attaccactg caaggtgaaa tcactattag tggtgctaaa aatagtgtcg
    22021 ttgccttaat tccagctatt atcttggctg atgatgtggt gactttggat tgcgttccag
    22081 acatttcgga tgtagccagt cttgtcgaaa tcatggaatt gatgggagct actgttaagc
    22141 gttatgacga tgtattggag attgacccaa gaggtgttca aaatattcca atgccttatg
    22201 gtaaaattaa cagtcttcgt gcatcttact atttttatgg gagcctctta ggccgttttg
    22261 gtgaagcgac agttggtcta ccgggaggat gtgatcttgg tcctcgtccg attgacttac
    22321 accttaaggc gtttgaagct atgggtgcga ctgctagcta cgagggagat aacatgaagt
    22381 tatctgctaa agatacagga cttcatggtg caagtattta catggatacg gttagtgtgg
    22441 gagcaacgat taatacgatg attgctgcgg ttaaagcaaa tggtcgtact attattgaaa
    22501 atgcagcccg tgaacctgag attattgatg tagctactct cttgaataat atgggtgccc
    22561 atatccgtgg ggcaggaact aatatcatca ttattgatgg tgttgaaaga ttacatggga
    22621 cacgtcatca ggtgattcca gaccgcattg aagctggaac atatatatct ttagctgctg
    22681 cagttggtaa aggaattcgt ataaataatg ttctttacga acacctggaa gggtttattg
    22741 ctaagttgga agaaatggga gtgagaatga ctgtatctga agacagcatt tttgtcgagg
    22801 aacagtctaa tttgaaagca atcaatatta agacagctcc ttacccaggc tttgcaactg
    22861 atttgcaaca accgcttacc cctcttttac taagagcgaa tggtcgtggt acaattgtcg
    22921 atacgattta cgaaaaacgt gtaaatcatg tttttgaact agcaaagatg gatgcggata
    22981 tttcgacaac aaatggtcat attttgtaca cgggtggacg tgatttacgt ggggccagtg
    23041 ttaaagcgac cgacttaaga gctggggctg cactagtcat tgctgggctt atggctgaag
    23101 gtaaaactga aattaccaat atcgagttta tcttacgtgg ttattctgat attatcgaaa
    23161 aattacgtaa tttaggagcg gatattagac ttgttgagga ttaaaccgta gaggtgttta
    23221 tgaatatttg gaccaaatta gcaatgtttt ctttttttga aacggatcgc ttgtatttgc
    23281 gtcctttctt ttttagtgat agtcaggact tccgcgagat agcttcaaat ccagaaaatc
    23341 ttcaatttat tttcccaacg caggcaagtc tggaagaaag tcaatatgca ctggccaatt
    23401 actttatgaa gtcccctttg ggagtgtggg caatttgtga ccagaaaaat caacaaatga
    23461 ttggttctat taaatttgag aagttagatg aaatcaaaaa agaagctgag cttggctatt
    23521 ttttgagaaa agatgcttgg tcgcaaggat ttatgacaga ggttgttaga aaaatttgtc
    23581 agctttcttt tgaggaattt ggcttaaaac aattatctat cattacccac cttgaaaatg
    23641 aagctagcca aagagttgct cttaagtctg gatttagttt gttccgtcag tttaagggaa
    23701 gtgatcgtta cacaagaaaa atgcgggatt atcttgaatt tcggtatgta aaaggagagt
    23761 tcaatgagta agcatcagga aattctaagc tatttggagg aattaccagt aggtaaaagg
    23821 gtcagtgttc gtagcatttc gaatcatcta ggagttagtg atggaacagc ctatcgggct
    23881 attaaagaag ctgaaaaccg tggaattgtg gagacccgtc ctagaagtgg aacaattcgt
    23941 gttaaatccc agaaagttgc tatagagaga ttaacgtttg ctgaaattgc agaagtgact
    24001 tcttctgagg ttctggctgg gcaagaaggt ttagagagag aatttagcaa gttttcaatt
    24061 ggtgccatga ctgaacaaaa tatcttgtct taccttcatg atggggggct cttgattgtc
    24121 ggagaccgaa cccgtattca gttgctagcc ttggaaaatg aaaatgcagt tctggttaca
    24181 gggggatttc aggttcatga tgatgtgctt aaactggcca atcaaaaagg gattcctgtt
    24241 ctaagaagta agcatgatac ctttaccgtc gcgaccacga tcaataaagc cttgtcaaat
    24301 gtccaaatca agactgatat tctgacagtt gagaaacttt atcgccctag tcatgagtat
    24361 ggttttctga gagagacaga tacagttaaa gattatttgg acttggttcg taagaatcgt
    24421 agcagccgtt tccctgttat caatcaacat caggtcgttg ttggcgttgt aaccatgaga
    24481 gacgctggtg ataaatcacc aagcacgaca attgataagg ttatgtctcg tagtctattt
    24541 ttggttggat tatcgacaaa tattgccaat gtgagtcaac ggatgatcgc agaagacttt
    24601 gaaatggtac cagttgttcg aagcaatcaa actttgcttg gcgttgtgac gcgacgagat
    24661 gtcatggaga agatgagccg ttctcaagtt tcggctctac caactttttc tgagcagatt
    24721 ggacaaaagc tctcttatca ccatgatgaa gtagtcatta cagtggaacc ctttatgcta
    24781 gaaaaaaatg gagttttggc taatggtgta ttggcagaaa ttctgaccca catgacccaa
    24841 gatttagttg ttaatagtgg tcgcaatctc attatcgagc agatgctgat ctactttttg
    24901 caggctgttc agatagatga tatattgcgc attcaggcac ggattattca tcatacgaga
    24961 cggtcagcta taattgatta cgatatttat catggtcacc agattgtttc aaaagcaaat
    25021 gtgactgtta aaattaatta gaaactagga gaaaagatga taacattaaa atcagctcgt
    25081 gaaatcgaag ctatggacaa ggctggtgat tttctagcaa gtattcatat aggcttacgt
    25141 gatttgatta agtcaggcgt agatatgtgg gaagttgaag aatatgtccg ccgtcgttgt
    25201 aaagaagaaa atttccttcc acttcagatt ggggttgacg gtgccatgat ggactatcct
    25261 tatgctacct gttgctctct taacgatgaa gtggctcacg ctttccctcg tcattatatc
    25321 ttgaaagatg gtgatttgct caaagttgat atggttttgg gaggtcccat tgctaaatct
    25381 gacctgaatg tctcaaaatt aaacttcaac aatgttgaac aaatgaaaaa atacactcag
    25441 agctattctg gtggtttagc agactcatgt tgggcttatg ctgttggtac accgtccgaa
    25501 gaagtcaaaa acttgatgga tgtaaccaaa gaagctatgt acaagggtat tgagcaagct
    25561 gttattggaa atcgtatcgg tgatatcggt gcggctattc aagaatacgc tgaaagtcgt
    25621 ggttacggtg tagtgcgtga tttggttggt catggtgttg gcccaactat gcacgaagaa
    25681 ccaatggttc ctaactatgg tattgcaggt cgtggactcc gtcttcgtga aggaatggtc
    25741 ttaaccattg aaccaatgat caatacaggc gattgggaaa ttgatacaga tatgaaaact
    25801 ggttgggcgc ataagaccat tgacggtgga ttgtcatgtc agtatgaaca ccaatttgtc
    25861 attacgaaag atggacctgt tatcttgact agccaaggtg aagaaggaac ttattaataa
    25921 aaagtgaaaa gactactgga agtttattat gaaaaaatcc agtagatctt ttcataataa
    25981 aacgcattgt atcaagtgtt aggggctgat atcatgcgtt tttctgcttt taagattttt
    26041 tccaactctg tttgtaagcg catcataaca aagggtctag gattcagggc tctcctccta
    26101 tatactatta gtaaagtaaa actaagggag gatattttag tgtcgcagtc tattgttcct
    26161 gtagagattc cacaatattg tcgttttgat tctaaaaaga gaaatggaat tctgtttaat
    26221 gttcgtattg ccaatcttaa atttaccttt tttagattag actccctgcg aaacaaaaca
    26281 tggtatagta gttctatgaa tgatgaagca agtaaacaac taactgatgc acgatttaag
    26341 cgttttgttg gtgttcagcg cacgactttt gaagagatgt tagctgtatt aaaaacagct
    26401 tatcaactta aacacgcaaa aggtggacga aaacctaaat taagcctaga agaccttctt
    26461 atggctactc ttcaatatgt gcgagaatat cgaacttatg aacaaattgc ggctgatttt
    26521 ggtatccacg aaagcaactt tatccgtcgg agccaatggg ttgaagtaac tcttgttcaa
    26581 agtggtgtta cgatttcaag aactcctctc agctctgagg acacggtaat gattgatgcg
    26641 acggaagtac aaatcaatcg ccctaaaaaa agaattagcg aatgattctg gtaaaaagaa
    26701 atgccacg
//
LOCUS       DBEROU010000030        25319 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.030, whole genome shotgun sequence.
ACCESSION   DBEROU010000030 DBEROU010000000
VERSION     DBEROU010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 25319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25319
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.030"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..78)
                     /locus_tag="ACL8CL_001367"
     CDS             complement(<1..78)
                     /locus_tag="ACL8CL_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HGS1330606.1"
                     /translation="MLRLYLENEIIELSNNLETIWVSVLD"
     gene            complement(136..519)
                     /locus_tag="ACL8CL_001368"
     CDS             complement(136..519)
                     /locus_tag="ACL8CL_001368"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330607.1"
                     /translation="MLVNEMEITDHRVDNLFEKGKNEIKDPIGTNSVLNKKIILQKIR
                     KLSNQPSGYWIGSLDERFLDHAIINQIDVTSEQIVLMSDGFYEFYQNNQNKTFEELIK
                     MRFNSSAIDPIYGKKDDASIVVIDV"
     gene            complement(691..987)
                     /locus_tag="ACL8CL_001369"
     CDS             complement(691..987)
                     /locus_tag="ACL8CL_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330608.1"
                     /translation="MVKINKICSIQGSSVENEDIVGSQNQYFWIIGGATDLYNSKEEI
                     GYSVSEVVHILSESLSVNCKESKTLKQIFETALLEVKDEIGLNSYELTEYNKMK"
     gene            complement(971..3130)
                     /gene="pfbA"
                     /locus_tag="ACL8CL_001370"
     CDS             complement(971..3130)
                     /gene="pfbA"
                     /locus_tag="ACL8CL_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HGS1330609.1"
                     /translation="MLKIVKKLEVLMKYFVPNEVFSIRKLKVGTCSVLLAISILGSQG
                     ILSDEVVTSSSPMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNS
                     ISSNGTIRSNSQLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAV
                     GDGIHDDRQAIQDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILN
                     GINIKNHPSIVFMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLI
                     NSSGAFAIGNSNNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQ
                     IISKESIQIEPLTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTL
                     STQNPSNIKILNNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVN
                     AYSYKNTKDLLDLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVIN
                     NNSKETEQPNIELLRVSDNLVVSENSIFGGKEGIVIEDSKGKITVLNNQFYNLSGKYI
                     SFIKSNANGKEPVIRDSDGNFNIVTENGLYKIVTNNLSDKNEKEKNKEEKQSNSNNVI
                     DSNQKNGEFNSSKDNRQMNDKIDNKQDNKTEEVNYKIVGDGRETENHINKSKEIVDVK
                     QKLPKTGSNKIMELFLTVTGIGLLLTLKGLKYYGKDK"
     gene            complement(4261..5487)
                     /locus_tag="ACL8CL_001371"
     CDS             complement(4261..5487)
                     /locus_tag="ACL8CL_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HGS1330610.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEVIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(5530..6222)
                     /locus_tag="ACL8CL_001372"
     CDS             complement(5530..6222)
                     /locus_tag="ACL8CL_001372"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS1330611.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTEKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(6395..8146)
                     /locus_tag="ACL8CL_001373"
     CDS             complement(6395..8146)
                     /locus_tag="ACL8CL_001373"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330612.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYGLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLAYANRSVALDALFLRPAMSLLKLLGYAVLMTYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQAAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLTLGG
                     TYHKMYSLQAGAMAYTL"
     gene            complement(8136..9881)
                     /locus_tag="ACL8CL_001374"
     CDS             complement(8136..9881)
                     /locus_tag="ACL8CL_001374"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330613.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQTAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDGWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(10087..10383)
                     /locus_tag="ACL8CL_001375"
     CDS             complement(10087..10383)
                     /locus_tag="ACL8CL_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HGS1330614.1"
                     /translation="MSASDVALKLRSQGIFQMKQVKRAVQEQNGQLIVVQMGDENPKY
                     PVVTDGVIQVDVLESIGRSEEWLLDNLSKQGHDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(10536..10991)
                     /locus_tag="ACL8CL_001376"
                     /pseudo
     CDS             complement(10536..10991)
                     /locus_tag="ACL8CL_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            11125..11952
                     /locus_tag="ACL8CL_001377"
     CDS             11125..11952
                     /locus_tag="ACL8CL_001377"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HGS1330615.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTVTFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDVKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            11964..12107
                     /locus_tag="ACL8CL_001378"
     CDS             11964..12107
                     /locus_tag="ACL8CL_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HGS1330616.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            12107..12346
                     /locus_tag="ACL8CL_001379"
     CDS             12107..12346
                     /locus_tag="ACL8CL_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HGS1330617.1"
                     /translation="MLEEGTKDQLAELTYPFGRGVNLSFGIKDVPKLYQKVMEANYPI
                     YRPLTKRKFRVSDPYIYPHKFAVLDPDGYFLRFSE"
     regulatory      12729..12824
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            13224..13805
                     /locus_tag="ACL8CL_001380"
     CDS             13224..13805
                     /locus_tag="ACL8CL_001380"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HGS1330618.1"
                     /translation="MKLLEERILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAATGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            13805..15067
                     /locus_tag="ACL8CL_001381"
     CDS             13805..15067
                     /locus_tag="ACL8CL_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HGS1330619.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LAAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGMPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKKRLPIYYAAGFLVLLGLLPKFGALAQIIPSS
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(15118..15579)
                     /locus_tag="ACL8CL_001382"
     CDS             complement(15118..15579)
                     /locus_tag="ACL8CL_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DpnD/PcfM family protein"
                     /protein_id="HGS1330620.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(15576..16340)
                     /locus_tag="ACL8CL_001383"
     CDS             complement(15576..16340)
                     /locus_tag="ACL8CL_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-2 restriction enzyme DpnI"
                     /protein_id="HGS1330621.1"
                     /translation="MELHFNLELVETYKSNSQKARILTEDWVYRQSYCPNCGNNPLNH
                     FENNRPVADFYCNHCSEEFELKSKKGNFSSTINDGAYATMMKRVQADNNPNFFFLTYT
                     KNFEVNNFLVLPKQFVTPKSIIQRKPLAPTARRAGWIGCNIDLSQVPSKGRIFLVQDG
                     QVRDPEKVTKEFKQGLFLRKSSLSSRGWTIEILNCIDKIEGSEFTLEDMYRFESDLKN
                     IFVKNNHIKEKIRQQLQILRDKEIIEFKGRGKYRKL"
     gene            complement(16521..16925)
                     /locus_tag="ACL8CL_001384"
     CDS             complement(16521..16925)
                     /locus_tag="ACL8CL_001384"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HGS1330622.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVSI"
     gene            complement(16995..18476)
                     /locus_tag="ACL8CL_001385"
     CDS             complement(16995..18476)
                     /locus_tag="ACL8CL_001385"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGS1330623.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLSRVGEGVLEVETNL
                     DKLIDLKDQLVEETVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYAYSPE
                     QAIEDFYQLSQKNDYIKLKAIARNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVDQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKAFRFAGFEQVKAGIVKWPMSVLRLTSDSKEDLINLADKILQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEHPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFVRVLEDAGVYKQTEQGQTAFMRFVEQVGILLD"
     gene            complement(18495..19673)
                     /locus_tag="ACL8CL_001386"
     CDS             complement(18495..19673)
                     /locus_tag="ACL8CL_001386"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HGS1330624.1"
                     /translation="MTQHLTAETLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGSGLSSSASLEILTGVVAEHLFDLKLERLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            19837..20844
                     /gene="galR"
                     /locus_tag="ACL8CL_001387"
     CDS             19837..20844
                     /gene="galR"
                     /locus_tag="ACL8CL_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HGS1330625.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAI
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIEDKRLENFRNYSQAKGIYHDELIFQGN
                     FTAQSGYDLMKEAIQNLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(20890..21927)
                     /locus_tag="ACL8CL_001388"
     CDS             complement(20890..21927)
                     /locus_tag="ACL8CL_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HGS1330626.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADAALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(21928..22281)
                     /gene="nmlR"
                     /locus_tag="ACL8CL_001389"
     CDS             complement(21928..22281)
                     /gene="nmlR"
                     /locus_tag="ACL8CL_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stress response transcriptional regulator NmlR"
                     /protein_id="HGS1330627.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(22525..23415)
                     /gene="czcD"
                     /locus_tag="ACL8CL_001390"
     CDS             complement(22525..23415)
                     /gene="czcD"
                     /locus_tag="ACL8CL_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter
                     CzcD"
                     /protein_id="HGS1330628.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            23548..24084
                     /gene="sczA"
                     /locus_tag="ACL8CL_001391"
     CDS             23548..24084
                     /gene="sczA"
                     /locus_tag="ACL8CL_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator SczA"
                     /protein_id="HGS1330629.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKDYETTTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLQRELEHHVYSVLADNLKEAHPNLPTSYLQHLVMSNFIETLTWWLKKGQD
                     FTDQEVVQFYLDLLIPKN"
     gene            complement(24250..25056)
                     /gene="pnuC"
                     /locus_tag="ACL8CL_001392"
     CDS             complement(24250..25056)
                     /gene="pnuC"
                     /locus_tag="ACL8CL_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HGS1330630.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDGKGWIKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
BASE COUNT         7545 a         5620 c         4532 g         7622 t
ORIGIN      
        1 atcaagcaca gatacccaaa tggtctctaa attatttgat aattctataa tttcattttc
       61 taaatataac cttaacattt aggtacctct tcttaacaaa gttcaatagt aacaattaat
      121 attttaaaca atatatcaaa catcaataac tacaatactt gcatcatctt tctttccata
      181 gattggatca atagcagaag aattaaatct catcttaatt aactcttcaa aagttttatt
      241 ttgattattt tgatagaatt cataaaagcc atcgctcatt aaaacaattt gttcactagt
      301 aacatctatt tgattaataa tagcatggtc taaaaatctc tcatccaacg aacctatcca
      361 gtacccactc ggttgattag ataattttct gattttttgt aaaataattt ttttatttaa
      421 aacactattt gtaccaattg gatcttttat ctcatttttc cctttttcaa ataagttatc
      481 tactctatga tccgttattt ccatttcgtt tactaacatg acgcagtcac ctagcatcat
      541 atactccaac tttttttctg aaagtttagc aaatataaaa gcaaatgttg gtaagcgata
      601 atatagtata ttgaaactag aatagtacac ctctacttct aaaacattgt tagaaatcga
      661 tttgactgtc ctgattgatt tgtcctatta ttatttcatt ttattatact ctgttaattc
      721 atatgagttt aaaccgattt catctttaac ctcgagtaaa gcagtttcaa atatttgttt
      781 aagagttttt gattctttac aattaaccga caaactttct gataaaatat gtacaacttc
      841 tgagactgaa taacctatct cctctttaga attatataaa tctgtagctc caccaataat
      901 ccaaaaatac tgattttgtg aacctacaat atcctcattt tctacggaac ttccttgtat
      961 cgaacaaatt ttatttatct ttaccataat acttcaaccc ttttagtgtc aaaagtaaac
     1021 caattcctgt cactgttaag aatagttcca taatcttgtt cgaaccagtc tttggtaatt
     1081 tttgttttac atctactatt tctttagatt tattaatatg attttcagtt tctctgccat
     1141 ctccaactat tttatagttt acttcttctg tcttattatc ttgtttattg tcgatcttgt
     1201 cattcatttg tctattatct ttacttgagt taaactctcc gttcttctgg ttactatcaa
     1261 ttacattatt tgaattagat tgtttttcct ctttgttttt ttctttttcg tttttatcac
     1321 ttaaattatt tgttacaatt ttgtaaagcc cattctccgt tacaatattg aaattaccat
     1381 cgctatcacg tataacaggt tctttcccgt ttgcattaga tttgatgaat gatatatact
     1441 taccggataa attataaaat tggttattta aaacggttat tttacccttt gaatcctcaa
     1501 taacaattcc ttctttacca ccgaatatac tattctctga cactactaaa ttatcactaa
     1561 ctcgtaataa ttcaatattt ggttgttctg tttccttaga attattatta attacatttt
     1621 ttgttacagt aatatctgat acttttccta aatattctgc actatctttt gcaactcgta
     1681 tcgcttttgt tttaggatcg gcaatattaa atatattttc ggcgataacc acctgtttat
     1741 ttaaatctaa taggtcttta gtgtttttat agctataagc atttactaaa gctgctccgc
     1801 tttctcgata atgtacactc tctcctttaa ctttcttatc aaagcgattt cctttgatta
     1861 atacatcagt gaatcctgta aaacgtacac ctgcatacat catgttatca aaatgattat
     1921 ttaaaatttt aatattagag gggttctgtg tcgacaatgt ttgatagtgt gtgccaattg
     1981 ctgttactaa ttccccagat ttatcacttt tgccaaaata ggaattttga atagtcacat
     2041 tttcagattt tttcccatca tcattcaagg cataaggaaa accttttcta gttaatggtt
     2101 caatctgaat gctctcctta cttatgattt gcccatcctt catcgttttg ggtaaggctt
     2161 gcccaagaaa acgagaatta tcaactaata catttttcga acctgcaatt tgaatagcat
     2221 gcccttgata actatccttg aatgttacat tttttatagt tacgttattt gaattcccaa
     2281 tagcaaatgc acctgaagaa tttataagtg gtagattttt tgctttagtt ccttcttcat
     2341 tcaaagcacc gttcatatca atcgtaccac cagaataact aatatcttct gttgggcccc
     2401 attctacttg cgcaccatca tccgtaaata aacctgtcat aaaaacaatg gaagggtgat
     2461 tcttaatatt tataccattt agaattgtag ctttctcatt caattctaag tgtgtatgac
     2521 tttttaaaaa aacaatttct tttactaaat aagttccttc aggaaaatat acatttcctc
     2581 cacctagccc ttgagctgca gcatctattg catcttgaat tgcttgtcga tcatcatgaa
     2641 tcccatcacc taccgcacca taatctttta cacttaaagc agtatcttca aattcttttt
     2701 tgataattct gtcacttata acatcttcct tataactctt attttcatta gtagatgtta
     2761 ctgtagattc aactgttctg ttgtctaatt gtgaattgga acgaatagta ccattagagc
     2821 tgatactatt aacacttaac gaattatcta aaccattagt ggttgaatta gaggcaatca
     2881 tttcagcaga acgattctga ttaacagttg gtgaattatc taaatcatta gtaattgcat
     2941 tagaagactc ttttgtagcc atcggtgaag aactagtaac aacttcatcc gataaaatac
     3001 cttggcttcc caaaattgaa attgccaata gtaccgagca agtccccacc tttaatttac
     3061 gaatactgaa tacctcatta ggaacaaaat atttcatcag aacctccaat ttttttacta
     3121 tttttaacaa ttgattatta cttttcttaa aatagataga atgacaccgc tgtcattatt
     3181 tttatcatat attttccaaa ttgaattgtc aaggaataac acaagaaaat attaaaattt
     3241 atactcttcg gaaatctctt caaactaggt cagtgccgcc ttgccctaag tctaggttac
     3301 tgacttggtc agttctatag tagactgaaa ctataatagt acacctctac ttctaaaaca
     3361 ttgttagaaa tcgatttaac tgtcccgatc aatttgtcct attcttgttt cattttacta
     3421 tatctacaac ctcaaagcag ggttttgagc tatctatggc caacttccta gtttgctctt
     3481 tagccttcca ttcaaaagtg atgctatctt tcttagcttg aatataaaat atcccattta
     3541 ctatatttaa atttgttgat atgttgtaaa ttataaatga aagaacaaat accttgtcat
     3601 acaagcattc gccagggata tttacctgaa tatcgctcat atggcggatt gaaactaaaa
     3661 cactacaata tatttcttaa atagaccttc tataaaagct agatttaacc tcgataaaaa
     3721 tagatgcagt gcttctaaaa tactggaata gggcatatag gtaatcgtag acaagtgttt
     3781 tatagattgt ctcgcaggag atctaatgat gtatagcaag catcactaaa catatgggat
     3841 atttgcgtta ttataatatc aacatgctat aaattctatc atgcaaaaga atgactagaa
     3901 catgaaagac aggcacaaaa ctttgaacaa tattctgaaa acaaaaaaaa gacgctagtt
     3961 tccacaatct cactcatgga aactcacgtc tttcaaaaaa tagggtgaat tgaaaactag
     4021 aacaagacac tagattttct aaatgatgtc tagtaaggat tctaaatgct ataccaaatt
     4081 gctcacatct tatttcaaac cacgggactc ccacaagaaa ggaaaatcct ataaaaattc
     4141 tctctctgag gagaaagagg cctcaactac aggtgtaaac tattatcaat tgaaaacaac
     4201 agtttttaca ctcaattaga ctttttaggg acaggatcac tttcgcttga ctgtagtttg
     4261 tcattttttt gataaagtta gcactttttc agaaactgtt ttgaaagtct caatgatata
     4321 gtctacttct tcatcgctta atttagtatg aagagggagg gtaatttcat tctcaaagaa
     4381 ggcataggcc ttaggatagt tcgtcatatc aaatccaaga ttcttatagg ctgtcaagag
     4441 aggaagcggt ttgtagtgaa cattacttgc aattcctgct ttagccaatt cttggatgat
     4501 gaggttgcgt tcttctaggc ttgctccttc tacacgggtg atgtagaggt ggcgtgaaga
     4561 ttcgacagtt tcagtcttgt gtgccaaagg atggatgcga gaacctgcaa aaccactatc
     4621 atagcggtcc acaatgtcct tacggcgttg caacaaactt ggatagcggt ccaattgtac
     4681 caaaccaagt gaagccatga tatcggtcat gttgcactta taggctggtg taacgatatc
     4741 gtattcccat gaccccagtt gcatcttggc aagagcatcc ttagtttgcc cgtgaaggga
     4801 aaggatttgg aattccttgt acatctcttc gtcatcaatc actggattgg ctttccaagt
     4861 cgcacttcca ccttctgccg ttgtaaagtt cttaacagca tggaatgaga aggaagtaaa
     4921 atcagcgata gaaccagaag gttgtccttt ataagtagat cccaaagcgt gggcactatc
     4981 agagacaatg acaatacggt taaaggcctt ttgccacttg cttgaagcgg taaagaagtc
     5041 acgttttttc tccacgactt ggaacaaacg gtcataatcg caaacaatcc ctgcgagctc
     5101 tactgggata atcaccttag ttttctcagt gatagcttgc tcaagcaggt catagtccat
     5161 ctcaaacgta tctgcttgga tatccaccat gacaggggtt gctcccacgt gcgtaatgac
     5221 actacatgaa gccgtatagg tcatggctgg aacgatgact tcatcaccag gtcccacttc
     5281 caaaacgcgt aaaatcaact ccagagcggc tgtcgcagag ttgagacaaa cagtcttagg
     5341 tgtctgtgtg taaagagaca agcggcgctc cagttctttt gttttaggac ctgttgtgat
     5401 ccaaccagaa cgcagggtat ccgctacttc agcaatttct gcttctgtga tatcaggcgg
     5461 tgaaaatgga atattgtaat ttggcattga tttgctcctt ttatctgtac tcattttctt
     5521 atgactactt tattttagta cctcaaacac ggtttgaaac atgattttga tgtccccaaa
     5581 gaaactaaac tctcggagat aggcgaggtt atagcgcatc ttttcaggaa gaacatgctc
     5641 cacataggcc tgatcaactg acagaccttt ctccgtcatt tgactgatga tggtgtcctc
     5701 atccttgtag ttgatgctgg ctggagaggt aattcctgct tgcaagagca aggttgccat
     5761 catttcaggg ctatactgct ctgtataacg tggcacttca ggtcgtgtac cgacaaagga
     5821 catctcacct ttaaggacat tgaccaactg aggcagttcg tccaaacgga cacgtcggat
     5881 gaaatttcca accttggtaa tacggctatc gttagcagaa gtcaccagac ttcctttttt
     5941 atccgcatcc gtcaccatgg tacggaactt ccaaatcttg aaccgacggt tgtactgggt
     6001 cacacgctct tgcttgtaaa tcactggccc cttgctatcc aacttgatcc aaatgctcaa
     6061 gatgagaaag atgggagaag ttagaaccag taagaccaag gccaaaaccc agtccaagca
     6121 acgcttgaaa atcagcgaac ccttcctttt agagacaagc tggtagtaag actcaacctc
     6181 gcttgatttc atttccacag gcaagtcttc ccatttcagc atgctgttct cctttgtttt
     6241 tattatacta tttgtcaaca tttttcatta taccacaaaa tggaaaaggc ggtgaaagaa
     6301 atctgtaatt tgaaggattt ccttctagta ctcaatgaaa atcaaagtac agctttgaga
     6361 ttgcagataa agctgacatg gtttaaagag attttcaaag agtataggcc atggcccctg
     6421 cctgcaaact atacatcttg tgataggttc ctcccagagt caagagttcc tcatgggttc
     6481 cactctcgat aatgcgtccc ttatccaaga catagatgca gttggcgtct tgaatagtag
     6541 aaaggcggtg agcgatagca atagttgttc ggccctgtct catcttcgcc agagaagctt
     6601 gaaccaaact ttctgtttca gagtcaatat tggctgtcgc ttcatccaaa atcaggattt
     6661 taggctggct ggcgactgtt ctagcaaagg caagaagctg gcgctgccca gtagagaagc
     6721 tcgaaccacg ctcggaaaca ggggagtcgt acccctgagg aagttcttga ataaaggaat
     6781 ctgcatccac aaaggctgcc gcagcctgaa cctgctcatc actgatttct tggtacatgg
     6841 cgatattgga cttaatagtt ccatgataga ggaagggttc ctgtaaaacc aaaccgatgt
     6901 tttttctcag ctcttcttga ctgaaatccc tgatatccac atcatccaag agaactctcc
     6961 ctgactggaa ttcataaaag cgcatgagga cattgataat agacgatttc cctgaacctg
     7021 tatgacctac aaaggcaatg gtttcaccct tattaacaga gaaagaaatg tcatccagaa
     7081 tcggatgttt accgtcatac gagaaacaca catgttcaaa acggatattg ccttctttga
     7141 ctttggcttg cccattttct tgaaggggct cataggtcct ctcatcgatt aaggcaaaga
     7201 cacggcctgc agaaaccata gacgtttgca gtgttgaaaa gttttgcgtc acctcaatca
     7261 aagggtcaaa gaggcggttg atgtactgga taaaagcata catggtcccg gccgttatcc
     7321 cgatagaaag accacgatag ccaaaatagg tcatcaagac agcataacct agaagtttca
     7381 acaaactcat ggcaggtctc aaaaagaggg catccaaggc tacagaacgg ttggcatagg
     7441 ccaagtgctc ttggttgatt tcatcaaatt ctgcctgcag gcgcttctct tgattaaaag
     7501 cctgaataat cctgattccc tcgatattct ctgccagctt actattgata tctgacaaga
     7561 gacttctggt tttctcgatg attttcactg acttttttcg atagagattg accaaaagga
     7621 aaatcaaggg gagaaagagc aagactaatg ctgtcaaacg aaaatccagc accaacatgg
     7681 tataaagggt tgtcagaaag ataaaaactg ctgagataaa gctggataaa atcccagaaa
     7741 acatatcact aatcgtctcg gtatcgttgg tcaaacgaga aacgatagaa cctgctggcg
     7801 tcttgtcaaa gtaagacatg cccagtttct ccatattggc aaaggcatcc cgacgaatat
     7861 ccctaacaat actgtaagac acgcgcgcaa agagaagatt gccgacatac tgaactacag
     7921 tttgtaggat gtagagacca tagtagacca gcaaaacggt cacggctagt tggttaagat
     7981 tgctgagata ctggtcgata aagtgggaag ccacaagggg aatgacactt ttaatgaccg
     8041 tcgtcgctag gagaaaactg agtgccaaaa aggtaaggag tccataaggc ttgagatagg
     8101 acatcaagcg cttcaataca gtccattgtt cttgtttatt ctgcatcttc ttctcccttc
     8161 atttccagct gctgagactg gtaggtttgg gcataccagc catccaaagc tagcaagtct
     8221 tcgtgcctgc ctcgttcgat aatttgacca ttttgcagga ctaaaatcaa atctgcatgg
     8281 acaactgcac tcaggcgatg ggcagtgata atggttgtct tgtcctttcg cgtctccttg
     8341 aggttgtcga taatcgcata ctctgtcttg gcatctacgg cggataagga atcatccaaa
     8401 atcaagatat cagggtctaa aatcatagcc cgactcatag ccaaccgttg cttttgacca
     8461 ccagaaagac tgactccttt ttcaccaatc agcgtatcaa atccttgagg catgtctaca
     8521 atatcttggt aaactcgggc tagcttagta gcttcctcga ccgctgaaag gggcaagtta
     8581 ggattgccaa agcggatatt gtctaggatt gaagtcgcaa aaagaaactg gtcctgagga
     8641 acatagccca tgagactgcg aaggtctgtc agacgatagt cccgaatatc gtgaccgttt
     8701 agataaatgg cgcccttatc cacatcgtat tcacgcaaga ggagcttgat taaggacgtt
     8761 ttcccagagc ctgtctgccc aaccaagccc agtgtttgcc cttttgccaa actaaagtga
     8821 atatccgtca gtgtttcctc attttcaaaa gcaaagctgt caatggcata ctccaaacgc
     8881 ccattttcaa taccatccag aggaaactcc gggtcttgta caggagattc ctgagacaaa
     8941 agattttcaa tccgctggta agaaaccttc cctcgctgag tagtattaaa gaggaagccg
     9001 atggccataa gaggccagac cagcatatcc aaatagctga taaaggtgac tagattccca
     9061 actgtaatct gcccttcctg aaccatcaag gagccaacca aaagcgttaa aacataggac
     9121 gaaccaacaa acaagagaac catagggtca aagagactat catatttcat ggtttgcagg
     9181 ttcttttgga aggttaattc attgactgcc tgaaaagact tcaactcgtc tgcctgataa
     9241 ccgaaagact tggtcacttt gatacctgat acggactcct gtaccttgtt attgagttca
     9301 gaaaaagcag tttgggattc gccaaaggcc ttatgagtct ttctccctag gcgactagtc
     9361 gcataggcca tgaaaggtag ggggagaatg gcaacaagag tcatctgcca tgagatgcta
     9421 aagagcatgg tcaacaaagt caccagagcc gtgatagagg catccaccgc agacatgaca
     9481 ccgccacctg ctaaacgagt caaggcattg atatcattgg ttgcgtgtgc catcagatca
     9541 cccgtccgat aggtttgata aaaggctgac gacatttttg tgaaatgctt aaacaagcga
     9601 gaccgcatga tctgtcccaa gcaataagag gtcccaagga tatacatacg ccacacatag
     9661 cgcaaatagt acataccaaa ggctgcaagt agcaagtaaa ataggctaag aaggaggtcc
     9721 tgctgggtta attgccccga tgtgatggca tcaatgaccc gccccataac cataggagga
     9781 atgagattga ggacggaaac caagaccagg gccacaatcc cgactagata acggcgtttt
     9841 tctaacttga aaaaccacca aagtttttga ataatggaca taaaatccct ttctgattgc
     9901 aaatggaagc ctgaggccaa tactcaatga aaatcaaaga gcaaactagg aaactagccg
     9961 caggctgctc aaagcactgc tttgaggttg tagatagaac tgacgaagtc agtaacctac
    10021 atacggcaag gcgacgttga cgcggtttga agagattttc gaagagtaca agaccccagg
    10081 tttttcttat tcataagtta cgactgtaac agcacccttg tcatattcag caataaagat
    10141 attggctaca ttgtcatgcc cttgtttgct gaggttatca agcaaccact cttcgctacg
    10201 accaatcgat tccaagacat ctacttgaat cacaccgtca gtcacaactg gatacttagg
    10261 attttcatct cccatttgca caacgatgag ttgcccattt tgctcttgca cagctcgttt
    10321 gacttgcttc atctggaaaa tcccttggct acgaagtttg agggctacat ccgatgcaga
    10381 caaaccaact gaacgacagg cttatgggtc aatattccca tttttgataa ggagagttgg
    10441 cttaccatca atcaaacgtt tcataaagtg aacattgttg ttgaactatt tgagagtcaa
    10501 gaccagtttg attaaaattt cgatataatt aagtgtcatt tgttcacatc caccagttca
    10561 attgcgtctg tcttatacaa ttcgactttc tctaacaggt acttgtctgg ctcacttccg
    10621 tttagggcac gataatagcg atctcccacc ttgataagtg cgccatctgt gtttgtggaa
    10681 gtattaacat agacttctga cttgtctact cccaaatctt tggaaacaac ctcgatgaaa
    10741 tgaagtgaag tttgaaattt attgttagaa acttgattgt tttgataagt cgtgatgctg
    10801 accaaaagca gagctaaata aggccagagc tgaaatcatg accaactcac gaaattcggt
    10861 tcccttttta tcatggtatg ccttaaaagc aaaaaaactg tcacagctaa tagaacaatc
    10921 ccaaagccaa ccatgatacc attttgctga ccgatttggc tgagtacata gtcataagag
    10981 tagaatttca tgtattttca ctccctttca taaaatcatt cttaattata aacgaaagag
    11041 agtaattttt caaaccatac gttggggaaa tagatctttt tttggtataa taaaatctat
    11101 aatctgaatg aaaaaggtaa ctttatgaaa cttatctcat ggaatattga ttccctaaac
    11161 gctgccctaa ctagtgactc agctcgtgcc aaattgtccc aagaagtcct acaaaccttg
    11221 gtcgctgaaa atgctgatat cattgctatc caagaaacca agctttctgc caaaggacct
    11281 acaaagaaac acgtggaaat tttagaagaa ctcttcccag gctacgaaaa cacgtggcgc
    11341 tcttcccaag agcctgcccg taaaggctat gctggaacca tgttccttta taagaaagaa
    11401 cttacaccca ctgtcacttt cccagaaatc ggtgcccctt ctaccatgga cttggaaggc
    11461 cgcatcatca ctctagaatt tgatgcattt ttcgtgaccc aagtttacac accaaatgct
    11521 ggcgatggtc ttaaacgctt ggaagaacgc caagtctggg atgtcaaata tgcggagtat
    11581 ttggctgaac tagacaaaga aaaaccagtc cttgcgaccg gtgactacaa cgtagcccac
    11641 aatgaaatcg accttgcaaa tcctgctagc aaccgccgtt cacctggatt tactgacgag
    11701 gaacgtgctg gatttaccaa cctattggca actggattta ctgatacctt ccgccatgtt
    11761 catggcgatg ttcctgaacg ctacacttgg tgggcacaac gcagcaaaac ttctaaaatc
    11821 aacaatacag gctggagaat cgactactgg ctcacaagta accgtatcgc tgacaaggtc
    11881 actaaatctg atatgattga ctcaggtgct cgccaagacc atacaccgat tgtcttggaa
    11941 attgacttgt aaggattttc tatatggact acaatgcggt cattcccgag tttcttgtat
    12001 cgaatatcga acagtcccgg tccttctact gtggtttact gggttttagg attgaatacc
    12061 aacgtccaga agaaaacttt ctctttctct tgaagagtgt caactaatgt tagaggaggg
    12121 aacaaaggat caactagctg aactgaccta cccctttggt cgtggagtca atctctcctt
    12181 tggcataaag gatgttccaa aactctatca aaaagtgatg gaggcaaact atcctattta
    12241 tcgtcccttg accaaaagaa agtttcgtgt cagtgatccc tatatctatc ctcataaatt
    12301 tgcagttttg gatccagatg gctatttttt aagatttagc gagtagaata ataaaaattg
    12361 ggaaaagtct ttaaagttag aaaaaggcat attgtcaggt gtttaaaaac ttgatagtat
    12421 gccttttatg atggaaaaat cttctaatac tcttcgaaaa tctcttcaag ccacgtcaac
    12481 gtcgccttac cgtaggtata tgatactgac tctgtcaatt ctatccacaa cctcaaaaca
    12541 gtgttttgag gttgtggata gcttcctagt ttgctctttg attttcattt agtataaatg
    12601 ttagcttaca atggagtttt tgttcaccct ctcttctttt tgttcgcttt ttaaaccaaa
    12661 atactaacaa aatagttgat ttctaaagtt atttagtgta aaataaaaac attggctcaa
    12721 gcctatttaa aattgaatat cgttttactt gtttatgtcg tgaattggca cgacgtttct
    12781 acaaggtgcc ggaacaccta acaataagta agtcagcagt gagatatggc cgtttttgcc
    12841 atgtctattt aggggactta cttagagatt aagtaggtct tttttgaata tttttaatat
    12901 ttcccttaat tagtgcggac gggaggtgaa attatcctgt gaataatttc aggaatccag
    12961 ggaatttcat ccctaatttt tgagccccta gctctttcat ttctaggctt aggctaaaat
    13021 agttccccga actattttac tctcaaaaat cccgtcatag acaataacaa gattattgtc
    13081 tatgatacca ctacaacggg aaatatttga taagactcgc ttcgctcgta atagttttat
    13141 caaatatata gtattagcaa aagggatcac tatagaaata tttaaacaac aagtaaatta
    13201 acttttagac tttaggaggt cttatgaaat tattagaaga gcgcatcctc aaggatgggc
    13261 atatcttggg tgataacatc ctcaaggtag attccttttt aacccaccaa gttgacttta
    13321 gcttgatgcg agagattggt aaggtttttg cggaaaaatt tgctgctact ggcattacca
    13381 aggtcgtaac cattgaagcg tcgggtattg ccccagccgt ttttacagct gaagccttaa
    13441 acgttcccat gattttcgcc aaaaaagcta agaacatcac catgaacgaa ggcatcttaa
    13501 ctgctcaagt ctactccttt accaagcagg tgaccagcac tgtttctatc gctggaaaat
    13561 tcctctcacc agaggacaag gttttgatta tcgacgattt ccttgctaat ggccaagctg
    13621 ctaaaggctt gattcaaatc atcgaacagg ccggtgccac agtccaagct atcggtatcg
    13681 tgattgagaa atccttccaa gatggtcgtg atttgcttga aaaagcaggc taccctgtcc
    13741 tatcacttgc tcgcttggat cgttttgaaa atggtcaggt cgtatttaag gaggcagatc
    13801 tctaatgcaa actcaagaaa aacactcgca agcagccgtt ctgggcttgc agcacttact
    13861 agccatgtac tcaggatcta tcctggttcc catcatgatt gcgacagccc ttggctattc
    13921 agctgagcag ttgacctacc tgatttctac agatatcttc atgtgtgggg tggcaacctt
    13981 cctccaactc caactcaaca aatactttgg gattggactc ccagtcgttc ttggagttgc
    14041 attccagtcg gtcgctccct tgattatgat tgggcaaagc catggtagtg gcgctatgtt
    14101 tggtgccctt atcgcatctg ggatttacgt ggttcttgtt tcaggcatct tctcaaaagt
    14161 agccaatctc ttcccatcta tcgtaacagg atctgttatt accacgattg gtttaacctt
    14221 gatccctgtc gctattggaa atatgggaaa taacgttcca gagccaactg gtcaaagtct
    14281 cttgcttgca gctattactg ttctgattat cctcttgatc aacatcttta ccaaaggatt
    14341 tatcaagtct atctctattt tgattggtct ggttgttgga actgccattg ctgctactat
    14401 gggcttggtg gacttctctc ctgttgcggt agctccactt gtccatgtcc caactccact
    14461 ctactttggg atgccaacct ttgaaatctc atctattgtc atgatgtgta tcatcgcaac
    14521 ggtgtctatg gttgagtcaa ctggtgttta tctagccttg tctgatatca caaaagatcc
    14581 aatcgacagc acgcgccttc gcaacggtta ccgcgcagaa ggtttggcgg tacttctcgg
    14641 aggaatcttt aacaccttcc cttacaccgg attttcacaa aacgttggtt tggttaaatt
    14701 gtcaggcatc aaaaaacgcc tgccaatcta ctacgcagct ggtttcctgg ttctccttgg
    14761 actgcttcct aagtttggtg cccttgccca aatcattcca agctccgtcc tcggcggtgc
    14821 catgctggtg atgtttggtt ttgtatctat tcaagggatg caaatcctcg cccgagttga
    14881 ctttgctaac aatgaacaca acttccttat cgcagctgtt tcaatcgctg caggtgtcgg
    14941 tctcaacaac agtaatctct ttgtcagcat gccgacagcc ttccaaatgt tcttctcaaa
    15001 cggaatcgtc gtagccagcc tactcgctat tgtcctcaat gccgtattaa atcataaaaa
    15061 gaaataagaa aaagaggtgc gagcctcttt tttgtgtgac aaaataaaaa catctaccta
    15121 ttcatatatg ctagtgtcat agtcctgaaa atcatctgca tcaagaacaa tttcacaact
    15181 cttatacttt tctcttacaa gtcgttcggc ttcatctgga ttttcagcct ctatacttac
    15241 taaacgtgat aaagtttctg taatttctac tgtatacttt ttcagtttta gattgctttc
    15301 caataattca cctattaaca ataatctatg ataggagcta tgtagccact ctggcaattt
    15361 atctcctact tcccacccat atttttcctt catcaaagct agttgttttt cgtcaccaaa
    15421 tttcatattt tcagataacc atctcacaca atcgggatct gataaggtac agacgattaa
    15481 acttaggtta tcgtaacggc tttcagtttc ataatcaggc cagatgactt ttaagaaatc
    15541 atacacctct tctgatgcaa caagttgttt cgttttcata atttccgata ctttcctcta
    15601 cctttaaatt ctattatttc tttgtctctt aatatttgaa gctgttgcct aatcttttct
    15661 ttgatatgat tgttcttaac aaagatattt tttaggtcac tttcaaaacg atacatatct
    15721 tcaagggtaa attctgaacc ctctatctta tctatacaat ttagaatttc tattgtccaa
    15781 cctcttgatg acagagagct cttccttaaa aataaacctt gcttaaattc ttttgtaact
    15841 ttttctggat ctctaacttg tccatcttgc acaagaaata tccttccttt agaaggtact
    15901 tgtgataaat caatgttaca accaatccaa cctgctcgtc tagcagttgg tgcaagtggt
    15961 tttctttgaa taatcgattt cggtgtaaca aattgcttcg gaaggacaag aaagttattt
    16021 acctcaaaat tttttgtgta agttaaaaaa aagaaattag gattattatc tgcctgcaca
    16081 cgcttcatca tcgttgcata agcaccatca ttgattgttg atgaaaaatt tccttttttg
    16141 ctctttagtt caaactcctc actacaatga ttacagtaaa aatctgctac aggccgatta
    16201 ttttcaaaat gatttaaggg attgttccca caattagggc aataagactg acgataaacc
    16261 caatcctccg ttaagatacg tgccttttgg ctattacttt tataggtttc tactaattct
    16321 aaattaaagt gtaattccat ttaaaaattc ctcactaata tgagaactca ctcgatataa
    16381 gcaattaaaa tctactataa ctagtatacc acatatatgt acctattaca tttgtatttc
    16441 atcactctct tttatatttc cttctaacat atctcataaa ttaaaaatga gtgagataaa
    16501 tttatcccac tcacctttct ttatatactt acccgtcttt cctcagacct ttggcctgtg
    16561 acaaacatgg taaaggttgc cttacagaca tttctgccct cttgattggt gatatccaca
    16621 tccatcacac aggttgtacg accttgatgg acacattctc ctttaatggt caatacatcg
    16681 tcgagttttc ctgccttaag gtagttgata gaggattgga gtgtcacccc atcaagtccc
    16741 agcgagataa ccaccaaacc actgatttgg tcacatagcg tgaatagata gccaccatgg
    16801 gcattgccat agtagttgag cgatgagttc actactttgg tcgtcaccac aacgtgacca
    16861 tctctcattt tttcaatttc gtaattttca aaggcagata tagcgtcaaa atgaaagtct
    16921 ttcatcgttt cctcctgata tttcaaacga cactatgata ctacttttta taggactgtg
    16981 caaggagaaa gctcctagtc tagtaaaatt ccgacctgtt ccacaaagcg cataaaggct
    17041 gtctgccctt gttctgtctg cttgtagact cctgcatcct caagtacacg cacaaagata
    17101 gcacccacag agtccttgac gattgcaagg gctttttctt tatccgttag gtctggatgt
    17161 tgggatttga gttggtctgc ccactcctga tgataatcgg caactgtaac agcttctcct
    17221 acaagatagc tagcgacttg ctctacttct tctttcagac gtggtggcaa gattgccaag
    17281 cccatgacct caatcaagcc gatattttcc ttcttgatat gttggacatc cttgtgggga
    17341 tgatagatgc catcaggatg ctctgctgaa gtctgattgt ctcgcaagac caagtccaac
    17401 tcaaactgtc catcacgctt acgcgctatt ggtgtgatgg tgtgatgcgg tgtcccgtct
    17461 gtctctgcta aaatctgcac actagaatct gaatactgac gccattcctg caaaatctta
    17521 tcagccaaat tgatcaaatc ctctttggaa tccgaagtca aacgtaggac agacatgggc
    17581 cacttgacaa ttccagcctt gacctgctca aaaccagcaa atcggaaggc cttttgcaag
    17641 ggagccaatt ccataggaaa tacgtgacgg cctccctgat aatgatcatg agttagaata
    17701 gagcccccca caatcggcag gtcggcatta gatccagcaa aatatcctgg aaactggtct
    17761 acgatagcca acagacgttc aaaactctga cgactaatgg ccatgggacg atgctggcca
    17821 tctaaaaaga tacaatgctc attaaagtaa gcatagggcg aatactggaa accccattcc
    17881 tgaccaacca tttcaaaacg gataatacgg tgattgctac gagctgggtg gttaactcga
    17941 ccatggtagc cctcattctc tagacaaagc tgacactgag gataattact agcttgcacc
    18001 aacttggctg ccacaatctc tttgggatct ttttcaggct tagagagatt gatggtaatt
    18061 tcaagttctc cgtagtcaga tggaacacga taagcgatat ttctagcaat ggccttgagt
    18121 ttgatgtagt catttttctg actgagttga taaaaatcct ctatcgcttg ctcaggagag
    18181 taggcatagg cttcccagaa gtcacgattg acctgactcg gacaaggagt caccaagtcc
    18241 atcaattcag taccaaggat ttcacgcgca gtctgactat cctcaatcgt ctctaatcga
    18301 acggtttcct caactagctg gtccttgagg tcaatcaatt tatccagatt ggtctcaact
    18361 tccaaaacac cttctcccac tcgtgacaaa acacggttgg tcaggtagat tcgatccatt
    18421 tcctcaaatg aactttcaga aatgacatgt gttacaaatt tatctactaa ggtcactata
    18481 gagcctcctt ttgactagtc aaggacgcga gtgccacctg caacttcagc gatatagaag
    18541 cttggagcgt atccaactac ttcctcgtag tgtttgccta cagcttcctt aaaggcctca
    18601 acagtatctt tttgaaccaa ggcaatggca cagccaccaa aaccagcccc tgtcatacga
    18661 gcaccgagaa ctccttcttg tgcccaagct gtgtgaacaa gggtatctaa ttccaaacca
    18721 gttacttcat aatcatgctc cagagaaacg tgtgacgcat tcatcaaacg accaaatgtt
    18781 tccaaatcac ctgcttgaag ggctgcttga gctttaaggg tacgttggtt ttcaagcaca
    18841 gcatggcgag cacgtttcaa acgattttca tctttaatca gatagctata ttggtcaacg
    18901 gcccactcgt ccaattcacc cagggtctga atatccaagg caacttgcaa ttcttccact
    18961 gctttttcac actcagcacg acgttcattg tatttagagt ccgccaattc acggcgtttg
    19021 ttggtgttca tgataacaac gacattgtcc ttcaaatcaa gtggcaccaa atcatactct
    19081 aaggtgttgg tatcaaggta aatagcacgt tgatcagccc ccataccaat agcaaactgg
    19141 tccatgatac cagagttgac tccgataaag ttattttctg tttgttttcc gattttaacc
    19201 aaatcgagac gctctagttt taaatcaaag agatgctctg ccacgactcc tgtcaagatc
    19261 tccaaggatg ctgaagaaga caagccagaa ccatttggga tattcccata aacataaaaa
    19321 tcaaaacctt tgtcaatcac gtgcccagct tcttgcaaga aatgaagaac cccttttgga
    19381 tagttggtcc agttgtgctc tttttcaaac ttgaggtcag cgagaggcac ttcaattatg
    19441 cccttgtcct caaagttagc tgagtagaaa cgcaagactt ggtcatcacg cttgcgagct
    19501 gcaccataag ttcctaagga aatagcagct ggaaaaacgt gcccaccgtt gtagtctgtg
    19561 tgttcaccaa tcaaattaat acggcctggt gaaaagaaag tttggtctgc ttcttgacca
    19621 aaaacagcaa gaaagtcttt gcgaagagtt tcagcagtaa gatgttgtgt catatgattt
    19681 ctcctttgac tgtccgttaa gccaccttaa cgtgtaatcg ttttcttcta ttgtatccaa
    19741 gggatttccc aaggtcaatc ctttttacta aaattttact aaactatcag taaaaagcag
    19801 aaaaatatga tatactaacc taatcaagga ggatttatgg ctaccttaaa agacattgca
    19861 cagctagcct ctgtctctat cgcgaccgta tcccgcgtcc tcaaccgcga ccagagccta
    19921 tctgttacag aagaaaccag acaccgtatt ttaaccgttg ctgaagagct gggctacacc
    19981 aagcacctca agacaggcga gtcccataaa cccaagcaaa agattgccat tattcaatgg
    20041 gtcagcgaac aaggggagct ggacgacctc tactactacc agattcgcct aggaatagaa
    20101 aaaagagccc aagagttgga ctatgatatc ttgcgctatt ttaatgacca tccttttacc
    20161 ttaagcgagg aagtgattgg gattctctgc atcggaaaat ttagccgagc tcagatttct
    20221 gcctttgaag aataccaaaa acccctggtc tttctagaca gtgatacact ttccctggga
    20281 catacctgca ttattacgga cttttacact gccataaaac aggttgtcga ttatttcctc
    20341 agccaaggaa tggaccgtat cgggattcta acaggccttg aagaaacaac tgaccaagaa
    20401 gaaatcattg aggataagcg gctggaaaat ttcagaaact acagtcaagc aaaaggaatc
    20461 tatcatgatg aactgatctt tcaaggaaac tttactgccc agtctggcta tgacttgatg
    20521 aaggaagcca ttcagaactt gggagaccaa ctcccaccag catttttcgc agccagcgat
    20581 agtttagcca tcggtgccct tcgtgcgctc caagaagctg gaatcagcct accagaccgt
    20641 gtcagcctca tttcctttaa cgacaccagc ctgaccaagc aggtttatcc tcccctctcc
    20701 agcatcacag tttatactga agaaatgggg cgagcaggta tggatattct taacaaggaa
    20761 gtcctccacg gtcggaaaat ccctagcctg accatgctgg gaaccagact gacattaaga
    20821 gaaagtaccc taaatcaaga ataggataac ataaaaaacg aatagagttc taaaactcct
    20881 attcgttttt tattcgatta caatcataga cttaattgtc ttacgttcat ccatatcttt
    20941 ataggcttgg tcgatatctt ccagtttata acttgaagta aagacgcgac ctggattgat
    21001 atcaccatca aggacggctt ttagtaaaaa ttgcttatcg tatgttgtag cagaagctgc
    21061 cccacctgct acagagatat tttgcataaa tgtcgaacca agagcacgat tattatagtg
    21121 tgggactcct acaaagccca tacgccctcc attatgaaga acacctagcg cctgttctat
    21181 agcagcctcc gtaccaacac attcaagtgc tgcatctgct cctccaccga ggatttcacg
    21241 caccttggta attccttctt gaccacgttc tgcaacaaca gctgtcgcac ctgactccat
    21301 agccatcttt tgacggtctt catgacggct cataaggata atttgtgatg ctccacgcat
    21361 cttagccgcg atgacagcac attgaccaac agccccatca ccgataacaa caaccttgtc
    21421 ccctttttga acatttgcaa cacgcgccgc atgatagcct gtcggcatga catctgcaag
    21481 agtcaaaagg gacttgagca tcccttctgt atagtcagaa ggttgaccag ggattttaac
    21541 cagcgcccag tttgcatagt ggaagcgaat atattctgcc tgaaaatcac cccccaaatt
    21601 attgccaata tgattgtcgc aagaaccgtc aaatccagca agacaggcat cacactcacc
    21661 acatccatgt gtaaaaggga caatcacaaa atcacctggt ttcaccgtcg taatggcttc
    21721 cccagcttct tcaacaatcc caatcgcttc gtgtccacta tttttgtgtc cagctttcgt
    21781 ttctggatta cggtacctcc ataaatctga accacaaacg cacgcacgaa ccacacgaat
    21841 aatcacatca tccgcttcta ttatttgcgg acgttcaatg ctagcaagtc caacctgacc
    21901 tgcctttgta tatactgctg atttcattta aaattttcct tccttataaa gtttaatttt
    21961 gagatttaaa cgatttaaag ctgtctgtag ctgagacaag cgctcctcta atttttgctt
    22021 ttcctcttct aaaataccaa gcctctcctc tctcgtttca tctccctttt ggtagagcga
    22081 catatagtca actaaactat ctacagagac acccgccgaa cgaaaacact taataaattc
    22141 cagcgcttcg atatcctgat cttgaaaatc acgaatccca gtagcagtac gagtaatcgg
    22201 tggcacaaga ccaacccgtt cataataccg aatcgtatcc gctgaaattc ccaacaaatc
    22261 actggcagat ttaatattca cacgctaacc tcctctcatt tcattcactg aattcacgct
    22321 attctaactc acttatctta ttatataact ttgagttgac tccaagtcaa gcattttgta
    22381 tctaatcgaa aaatttttat cattaacaat catatatctg gagtttatac aatgctcttc
    22441 tcttaccaat ttttgaaata tttaaataat aaagaaatta ctctattcca aataagatag
    22501 tacccaagta tttttcactt ttttctaatg ttgatgctca taactccgtt ccaagtcaca
    22561 cacctttcgc ttatgggttt ggtgagtttc taggtcagca tcaatttcaa tggtaatatt
    22621 ttgaaaacca caatctttta ggaaaattcg aatagactct ttacaagttt ccatatgttc
    22681 catttctttt agacaaacat gtacaatggc atttttttcc aaagcatcca tagtccagag
    22741 attaagctga ttaaggctgg ccacattgtc caatcgctcc aggccactct ttacttgctt
    22801 gatatcaaga ccttctggca cagcatccaa gaaaatcttg agtgtagacc aaaaacgtgg
    22861 aagggctttt gaaagaataa agaaagaaat gacaagggac aaaagaggat ctaggatata
    22921 ccagtccgta aatcgaagaa caatcgccat caggataaca gctacccacc ctagcgtatc
    22981 ttccagaaaa tgcaaactca gaatagactc attctttgtc tttcccttac caaccaccag
    23041 actcgctaac agattgatag taatcgcaat aattcctaac cagagaatcc cctcatcatt
    23101 gactggttgc ggatgcaaaa tcttcgtgac attttccaaa atgactagaa cagagcccgt
    23161 tacgagaatc acagctgtta ccaaggctcc tagcaggcta aaccgcttat agcccaaggt
    23221 gtactgattg tcttcttcac gattggagat tgtttctaga aaagctgata ttccaattgc
    23281 aatcgcatct cccaagtcat gcacagagtc agcaagaaca gcgctagaac caaatactcc
    23341 acctgcaata aactcaacaa tggcataagt caaatttaag aaaaaagcca cccaaacagc
    23401 atattttgcc ttcatatttc tcattccttt gttataatag atttatgaac accttgttca
    23461 ttatcattat ccactaaaac tcaaagaaag gatagaagca aaacgtcagc tttattcagt
    23521 tctgaacaat ttgcctcaag tgtccatatg actaacattg accgccgtat cagcaaaacc
    23581 aaaaaagcca tctatcaagc ttttatacaa cttttgaatg ctaaggacta cgagaccact
    23641 actgttcagg atatcattga tctcgcagat gtgggacgat ccacctttta ctgtcactat
    23701 gagagtaaag agcttcttct ggatcagctc tgccgctacc tcttccatca tctttttgaa
    23761 agagagcaag ccatttcaac tgaggactac ctcgcccacc tctttctcca ttttcataaa
    23821 aaccaagacc atatcaccag tctgctattt tccaaaaatg actacttcct ccgtcaactc
    23881 cagagagagc ttgagcacca cgtctattcc gtgctagctg ataatttaaa agaagcccac
    23941 cctaatctgc ctacttctta cctccaacac ttggtcatgt ccaactttat cgagacattg
    24001 acctggtggc tcaaaaaagg tcaagatttc acagaccagg aagttgtcca attttatcta
    24061 gaccttctca ttcctaaaaa ttgaatatag agtaaagctc agttgtctta tttctaggtt
    24121 actgagtttt ttatcttttc aacaacaaaa gaggacccgc cgatcctctt tttcatacta
    24181 taaatccttg attatcaact atatctgttt taatcgaaat ctcaaaacag cactttcaaa
    24241 catcttttcc tagttaagta aatcagtatt ctgcttagct gccttgctcc attgatacca
    24301 accaactaga ctgttaatga gataaattag atatttccct tgaatttgca ggctttctcc
    24361 ccaccagaga tagattgaaa aaacattggt agccgcccag aatatccact gttcacggta
    24421 aacagctgtc atgaggattt gccctacccc attggttgca tctgtgattg aatcacgata
    24481 gggacgattg gcaccaatag actgataaat gaagccaaag gccaaccacc aaagcacact
    24541 aatggaaaga tactttatcc agccctttcc gtccagttta cgcgcgacaa actcctgctt
    24601 ttccttctta aactgtgcct gataaatcca aactagaagt ccaattggct gcatgactgt
    24661 gaagtaaagt gtcgtcagca cctcaccata aaagcctttc tgtagggcca aaataaggta
    24721 aataacagag ttaatcaagc caaaaagata attacttgct cgaccttccg atacaaagat
    24781 tacacagata atccctgtca agctacaaat catcccaatc cagtcaacaa tacgatgttc
    24841 gtaaaccaac tccagccaga gaggaaaact tcctaaaacc agcaaataaa tccactgggc
    24901 aaaactacga tgggcaaaga ggtcatccca gatagccttc atagttcctg aaaatcctaa
    24961 atcagccata gccgcaacca tacgacggta accacctgac atttcaccta gggttgtttt
    25021 gatattttca attttctttt gcaaataagt atgcatcatt tctccttttg tttttaaaga
    25081 gccgtgtctg gatagacttt cggacgcaac gctctattag ataatgaact gcctatacac
    25141 aagatttcta accttagtcg acatgagctg aaacctctta tttgttaagt agttcacaaa
    25201 atattataca cctattttat gaatagtcaa ctgtctttac agtaaaattt tagaaaatca
    25261 tgaaaatttt ctctttcttt ccattttaag tgacattcag tcattctcac atcaaaaaa
//
LOCUS       DBEROU010000031        25061 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.031, whole genome shotgun sequence.
ACCESSION   DBEROU010000031 DBEROU010000000
VERSION     DBEROU010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 25061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.031"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(172..3348)
                     /gene="carB"
                     /locus_tag="ACL8CL_001393"
     CDS             complement(172..3348)
                     /gene="carB"
                     /locus_tag="ACL8CL_001393"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HGS1330631.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEVPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(3660..4739)
                     /locus_tag="ACL8CL_001394"
     CDS             complement(3660..4739)
                     /locus_tag="ACL8CL_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HGS1330632.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTTAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(4789..5712)
                     /locus_tag="ACL8CL_001395"
     CDS             complement(4789..5712)
                     /locus_tag="ACL8CL_001395"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HGS1330633.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGAELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(5731..6252)
                     /gene="pyrR"
                     /locus_tag="ACL8CL_001396"
     CDS             complement(5731..6252)
                     /gene="pyrR"
                     /locus_tag="ACL8CL_001396"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HGS1330634.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(6463..7092)
                     /gene="nth"
                     /locus_tag="ACL8CL_001397"
     CDS             complement(6463..7092)
                     /gene="nth"
                     /locus_tag="ACL8CL_001397"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HGS1330635.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(7092..7634)
                     /locus_tag="ACL8CL_001398"
     CDS             complement(7092..7634)
                     /locus_tag="ACL8CL_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HGS1330636.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENGALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFASLQGLFDGDE"
     gene            7804..8121
                     /locus_tag="ACL8CL_001399"
     CDS             7804..8121
                     /locus_tag="ACL8CL_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HGS1330637.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSPLIGLGSLKLNNQARTAWRNYDKIHYEHVKHVLSLYGPV
                     FGF"
     gene            8360..9919
                     /locus_tag="ACL8CL_001400"
     CDS             8360..9919
                     /locus_tag="ACL8CL_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS1330638.1"
                     /translation="MHYEHSRKGNHMIKINHLTITQNKDLRDLVSDLTMTIQDGEKVA
                     IIGEEGNGKSTLLKILMGEALSDFTIKGNIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQNVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9963..10862)
                     /gene="htpX"
                     /locus_tag="ACL8CL_001401"
     CDS             complement(9963..10862)
                     /gene="htpX"
                     /locus_tag="ACL8CL_001401"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HGS1330639.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTIAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIIMLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(10864..11424)
                     /locus_tag="ACL8CL_001402"
     CDS             complement(10864..11424)
                     /locus_tag="ACL8CL_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HGS1330640.1"
                     /translation="MTWIILGVIALIVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            11518..12231
                     /gene="rsmG"
                     /locus_tag="ACL8CL_001403"
     CDS             11518..12231
                     /gene="rsmG"
                     /locus_tag="ACL8CL_001403"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HGS1330641.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12534..13817
                     /locus_tag="ACL8CL_001404"
     CDS             12534..13817
                     /locus_tag="ACL8CL_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HGS1330642.1"
                     /translation="MKQESTVDLLLDVDQRPSDGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLKANWFE
                     IPGFYLPFSTGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLLGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(14012..15583)
                     /gene="ffh"
                     /locus_tag="ACL8CL_001405"
     CDS             complement(14012..15583)
                     /gene="ffh"
                     /locus_tag="ACL8CL_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002902912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HGS1330643.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15595..15927)
                     /locus_tag="ACL8CL_001406"
     CDS             complement(15595..15927)
                     /locus_tag="ACL8CL_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014637934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HGS1330644.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDDFLQEQIEI
                     LTSIDNRE"
     gene            complement(16017..16394)
                     /locus_tag="ACL8CL_001407"
     CDS             complement(16017..16394)
                     /locus_tag="ACL8CL_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HGS1330645.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKGDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYIPTPMGMQEFIIQTSRYQVDGQKIE
                     LAYQLQNQEGHPFASYQLEITWG"
     gene            16466..17770
                     /locus_tag="ACL8CL_001408"
     CDS             16466..17770
                     /locus_tag="ACL8CL_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HGS1330646.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFENLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPVENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKDFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSHRFVNRKVFKSITFSQEDQDQLTSMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSLIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLHLIENDHFTPNKN"
     gene            17783..18589
                     /gene="yidA"
                     /locus_tag="ACL8CL_001409"
     CDS             17783..18589
                     /gene="yidA"
                     /locus_tag="ACL8CL_001409"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HGS1330647.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDQGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            18632..19630
                     /locus_tag="ACL8CL_001410"
     CDS             18632..19630
                     /locus_tag="ACL8CL_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003712 - transcription coregulator
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAP domain-containing protein"
                     /protein_id="HGS1330648.1"
                     /translation="MNFFSKLFNLKQNNHNRDTNSDCNNFYLNELECGLTPGQLILID
                     WTQKTGRNYNFPRYFKYSLQIDPESTHNQLYKLGYFTKNKTLSYLTVVELKTILSKHN
                     LATSGKKAELITRIINNVNIDNLDIPFEFKLTKEAQNLIIEHSDYIKAYYDKDITMED
                     YCKEKNNISFKATFGDIKWSLLNKQAHRNTVSGDFGCLSNTRKAQGRHLEQEGNIKHA
                     LIYYIESLIITISGLENNFSATDYPVYYPDSIPDYSLKHIQTLMESLSDDDYDFAFDE
                     ALFRFSILNANHFLSKEDIDYLRVNLPRSTAEEINNYLKKYECYSPLNNLELDDFE"
     gene            complement(19797..19868)
                     /locus_tag="ACL8CL_001411"
     tRNA            complement(19797..19868)
                     /locus_tag="ACL8CL_001411"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19833..19835),aa:Arg,seq:cct)
     gene            complement(19910..20257)
                     /gene="rplS"
                     /locus_tag="ACL8CL_001412"
     CDS             complement(19910..20257)
                     /gene="rplS"
                     /locus_tag="ACL8CL_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HGS1330649.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(20376..20705)
                     /gene="crcB"
                     /locus_tag="ACL8CL_001413"
     CDS             complement(20376..20705)
                     /gene="crcB"
                     /locus_tag="ACL8CL_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGS1330650.1"
                     /translation="MVIVYLAIACGFGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLTLTYIGGLVA
                     IFLGILL"
     gene            complement(20699..21073)
                     /gene="crcB"
                     /locus_tag="ACL8CL_001414"
     CDS             complement(20699..21073)
                     /gene="crcB"
                     /locus_tag="ACL8CL_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HGS1330651.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFVVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(21070..21336)
                     /locus_tag="ACL8CL_001415"
     CDS             complement(21070..21336)
                     /locus_tag="ACL8CL_001415"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HGS1330652.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(21452..21895)
                     /locus_tag="ACL8CL_001416"
     CDS             complement(21452..21895)
                     /locus_tag="ACL8CL_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HGS1330653.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRIFVSTGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVV"
     gene            21999..22934
                     /locus_tag="ACL8CL_001417"
     CDS             21999..22934
                     /locus_tag="ACL8CL_001417"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HGS1330654.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            23030..23272
                     /locus_tag="ACL8CL_001418"
     CDS             23030..23272
                     /locus_tag="ACL8CL_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HGS1330655.1"
                     /translation="MKKDIHPEYRPVVFMDTTTGYQFLSGSTKRSNETVEFEGETYPL
                     IRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(23329..23907)
                     /locus_tag="ACL8CL_001419"
     CDS             complement(23329..23907)
                     /locus_tag="ACL8CL_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron permease"
                     /protein_id="HGS1330656.1"
                     /translation="MVRNYWKAKSWLLVLALSFLILSPAGAQESLSFFFVKITDASKA
                     VKNGGQTETQKLVTKMASDFERVENKDSEVGKIVKEKLALSGDITEAKLTEISSALLA
                     FEKEQNPVDLDAEKEKLVNRLSPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVV
                     RDNSTAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(23882..24893)
                     /locus_tag="ACL8CL_001420"
                     /pseudo
     CDS             complement(23882..24893)
                     /locus_tag="ACL8CL_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259617.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
     gene            complement(24896..25061)
                     /locus_tag="ACL8CL_001421"
                     /pseudo
     CDS             complement(24896..25061)
                     /locus_tag="ACL8CL_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
BASE COUNT         7483 a         5560 c         4694 g         7324 t
ORIGIN      
        1 tttttgaaaa atcttgatat gatgcgtttt ataattttaa agactttttt ctatagtaga
       61 ttgaaataag atgcgaacaa atcaattaga aaattcaaat taatttctag aaatatttta
      121 gtatccctgt gtactgttct aaattcagtc tgctatacct tatttttcta tttaaatcgc
      181 ttctgtaaca aagctacgac tttcaagtac cttaagcatg gcattagctg tatctagcgc
      241 tgtgaagagg ggcaccccgt gttcaatggc tgaacgacga atttgctcac catcttcgtc
      301 agcagttcgt tttgttccta ctgtgttaat gatagcttga attcttcctt tgcgtacaaa
      361 acttgggata tccttatcgt catcaccaat cttaccaaca ggttgggctt gcaagccatg
      421 actagcaaag aaggctgctg tcccttctgt cgcaaggatt ccataaccaa tgttttggaa
      481 acgacgagcc aagttcaagg cttcttcttt ggcatcatca gcgatggtaa agacgacatt
      541 accaaaagtt ggcaagtgta gataagaagc ttcaaaggct ttatagagag ctttttccaa
      601 agtagcatca gaacccataa cttcacctgt tgacttcatt tcaggaccga gcaagctgtc
      661 taccttagct agtttggtaa aggagaagac aggtgccttg atatgaacac gggtgctttc
      721 agggtaaagt ccattttggt agccaagttc tgataaactt tgaccaagaa tgagcttggt
      781 cgctacttga gccataggaa tattggttac cttagatagg aatggaacgg tacggctggc
      841 acgtggattg acctcaataa cgtagacttt ttcatccttg ataacaaact ggatgttcat
      901 cattccaagg cagtgaagac cgattgctaa gcgtttggtg tagtctgcga tggtctcctg
      961 aaccttttgc gacaaggttt gtggtgggta aacagccatt gagtcacctg agtggacacc
     1021 agcacgttcg atatgctcca tgataccagg aatgagtaca tttttaccat ctgaaatggc
     1081 atcaacttcg cactcttgcc caacgatata agagtcgaca agaactggat ggtctggact
     1141 agccttaaca gcagttcgca tgtaagaacg aaggtcttct tcgttttcaa cgatttccat
     1201 ggcacgtcca ccaagtacat aagatgggcg gacaagaact gggaagccaa tcttacgagc
     1261 tgcaagagct gcttcttctt cattggtagc cgtttgtcct ggtggctgtg gaatatccaa
     1321 ttctttgaga gcttgctcga agaggtcacg gtcttcggca cggtctaggt cagcaacctg
     1381 tgtaccaagg atggtcacac ctgcttttgc caatggctcc gcaaggttga tggctgtttg
     1441 accaccgaac tgaacgataa ctccctttgg ttgttccaag tcaatgacgt tcataacatc
     1501 ttcgaatgtc aatggctcaa agtaaagctt atctgataca gagaagtctg ttgaaacggt
     1561 ctctgggttt gagttcatga tgatagcttc ataaccagct gcctggatag ccttaacaga
     1621 gtgaacggtt gcgtagtcaa actcaacccc ttgaccgata cggattggac ctgaacctag
     1681 gacaagtaca gattctttat cagatctgat agattcattt tcccaaccat aggttgaata
     1741 gaaatatggc gtttcggagt cgaactctgc cgcacaagta tctaccatct tatagactgg
     1801 gacaatcttg ttttccaaac gaagttggcg aactttatca tcagtcgttc cccagagttc
     1861 agcaatctta cggtctgaaa aaccattaag tttggctgtt ttcaaaactt ctagatcttg
     1921 tggatgagca cccaattctt gctcaatttc aaagatatgc aagagtttat caagatagaa
     1981 gatatcaatt tttgtaagct ctgcaatttc ttcaggtgtg tagccacgac gaattgcttc
     2041 tgatacgtag aagagacggt catcttgggc tttgacaacc ttttcaatca aggcatcatc
     2101 agaaactgct gcaagttcag gtacttcatt gtggtgcacc ccaatttcaa gggatcggca
     2161 ggccttgaga agagattcct cgatgttacg accgattgcc atgacttctc cagtcgcctt
     2221 catttgtgta ccgagacggc gttcaccctt ttcaaacttg tcaaatggga aacgtggaat
     2281 cttagcaact acgtagtcaa gggctggttc aaacatggca taggttgaac ctgtaactgg
     2341 gtttataacc tcatccaagg tcaaacctac tgcaatcttg gcagccaact tagcaatcgg
     2401 atatcctgtc gctttagaag caagggctga cgaacgtgat acacgagggt ttacttcgat
     2461 aacataatac ttgaagctgt taggatcaag agctagctga acattacatc caccttcaat
     2521 cttgagggcg cgaataatgc tcaagctcgc atcacgaagc atttggtttt catagtctga
     2581 catggtttgc gcaggggcaa atacaatgga atcccctgtg tgaatcccaa ctgggtcaaa
     2641 gttttccatg ttacaaacaa ccaaggcatt gtcagctgag tcacgcatca cttcgtattc
     2701 aatttccttg aaaccggcaa tcgaacgctc aatcaaacat tgggtaacag gtgacaattt
     2761 caaaccattt tcagtgattt cacgcaattc tttttcgttg gcacacatac caccaccagt
     2821 accaccaagg gtaaaggctg gacgaacgat gactgggtag ccaattgtcg ctgcaaaggc
     2881 aactgcttct tctactgtgt taacaatttc agattctgga atgggttgct caagctcttc
     2941 catcaattgt ttaaagaggt cacggtcctc cgcttggtca atggcagata atttggtacc
     3001 cagaagttca acgccaagct cgtctaggat accattttta gataattcca tggccatgtt
     3061 gagacctgtc tgaccaccga gtgttggtag caaggcatct ggaccttcct tacgaagaat
     3121 acgtgtcaca aactcaagtg taatcggttc aatgtaaacc ttgtcagcaa tttccttgtc
     3181 cgtcatgatg gttgcaggat ttgagttaac caaaacaacc tcataacctt cctctttcaa
     3241 cgacaagcaa gcctgagtcc cagcgtagtc aaactcagca gcctgaccaa taataatcgg
     3301 accagaacca atcaccataa ttttttgaat atcagtacgt ttaggcatag tttatgatac
     3361 aaagcccgta aagaacacag tgaaaatagg aaactcggcg cagacgcctt cagcgtcaag
     3421 acgatgttta tctttttcac acagttctta gggcgtgttc acttccgcga acaatgtatc
     3481 ttctcctttc tattcgtcgc ctctcagggc gacattaaat aagatacaaa ggacgaatag
     3541 aaagcgattg aattttagga aatcaaggaa ggattgacaa tccaagttgg tttctctaca
     3601 ttctgagctt tccgtccgtg ttcagttaca taaattctcc gacgagcttt tactcgttct
     3661 tagtttgatt gtttaaaaac ttccatcatc tcgataaact cgtcaaatag gtagctagcg
     3721 tcgtgtggcc caggagctgc atctgggtgg tattgaacag agaaagcagg ttggtatctg
     3781 tggcgcacac cttccactga cttgtcattg atttcttcgt gggtaataat caagtgctct
     3841 ggcaaatcct cgcggctgac tgcataacca tggttctggc tggtgaagtc tactcgtcct
     3901 gttgcgattt cacgtaccgc atggttgaat ccacggtggc caaacttcat cttataggtc
     3961 ttagccccgt ttgccattgc aaagagttgg tgtcccatac aaataccaaa gattggaatt
     4021 tttccttgta caccgcgaat catgtcgagt gcttgtggaa cgtcttctgg gttacctgga
     4081 ccatttgaca acataactcc gtcaggattg agatggagaa tttcttcagc cgttgtcgaa
     4141 taaggaacaa ctgtcacgtt acagttgcgt ttagaaagtt cacgtaggat tgagtgcttg
     4201 agaccaaagt ccactagcac cacgctcaaa ccaactcctg gagctggata agacgtttta
     4261 gtagaaacct gtttgatatt gtctgtcggt aaaactgttg cttggagctg gtccgtcaca
     4321 tggtccatac tgtccccaac atgggtcaag gttgcacgca tagtaccatg cttacggata
     4381 atcttggtaa gagcacgcgt atcaattcct gaaatccctg gaattttctt ggctttcaaa
     4441 aattcatcca aggtcatttg gttgcgccag ttgctagctc tacgcgcttc ttcaaaaaca
     4501 acgactccct tacaagttgg aataatggat tcataatcat cacgattaat accataattt
     4561 cctaccaaag gataagtaaa ggtcaagatt tgtccattat aagactggtc tgtaatggat
     4621 tcttggtagc cggtcatccc tgtattaaag acgatttcgc ctgttacatc aatatctgct
     4681 ccgaaggcct tgccttcaaa aactgtgcca tcttctaata ctagaattct ttttgtcata
     4741 ttttcacctc tcgtggacgc tcactggcgt cttttaacgt cttgtgtttt agttggcgtt
     4801 tctactcgct agtacggatt ctaagattgc cattcgaaca aagacaccat tggtcatttg
     4861 ttggacaatc cgtgattttg gtgcttcaac caagtggtct gctatttcta catcacgatt
     4921 gattggagct gggtgcatga ggattgctgt ttctttcaaa cgatcgtaac gttcttgagt
     4981 caagccatgt tgggcatggt agtcttcttt tgaaaataca gctccactat catggcgttc
     5041 gtgttgcaca cggagaaaca tcatgacatc aacctgatca atgatttcat caatggttac
     5101 aaactgtcca tagtctgcaa actcttgact tctccattcc tcaggtccag cgaaaaagag
     5161 ttcagctccc aagcgtttca aaatctgcat attggatttg gcaacgcgtg agtggtccaa
     5221 gtcacctgca atagcaactt taagaccctc aaagtggcca aattcctcat aaatggtcat
     5281 caaatcaagc aagctctggc tagggtgttg gcccgaacca tctccaccat tgatgatgga
     5341 agtcgtaatc gttggactag caatcaattc tctatagtag tcgacctctg gatggcgaat
     5401 cacacagaca tccactccta aagcagacag agtcaaaatg gtgtcataaa gtgtctcacc
     5461 cttattaacc gagctagtct tcacatcaaa gtcaagtcgt tccaatccaa gtttaatctc
     5521 tgcgacttca aaggacttat gtgtccgtgt agaatcctca aagaagagat tggaaacaat
     5581 cggatggtct tcatagggaa gctgggctcc atttttaaac tcaattcctc gcttgatcaa
     5641 tttcattact tgatcgacag tgaggtcttc catggacacc acatggttca atgcttgttg
     5701 attttctgac atggctactc ctttaacttt ctaagcttct tcagtaatca gaactctgtc
     5761 ttggtcatca agttctgtca tctctacgat gatttcttca gaacgactgg ttgggatatt
     5821 ttttccaacg taatctggac ggattggcaa ttctctatgt ccacgatcga ctagaactgc
     5881 taaactcaca cgcgcaggac gaccatgacc gacaatatta tcaatagcag cacggatggt
     5941 acgacctgta tagagcacat catccaccaa gataacttcg cggtctgtca catcgacaga
     6001 aaccaaagaa gtatcttctc cacttttaac atcatcacgg aaaggtttag tatccaattc
     6061 cacaacagga actgaaagat tttctaactg cttcaaacgt tcttggattc ggtgggcaat
     6121 aaagacacca cgagttttaa taccagccaa gacgatctta ttcaaatctt tgttgcgttc
     6181 gataatctca taagtaatac gcgtaatcgc tcgtttgacg gtcaattcgt ctacaacttc
     6241 ttttgttttc atgacaaacc tccaaaaaga aaagtctcct taaacaagga gacttgaaat
     6301 ttatagccaa gcgagcccta ctgcacacag tatagacttc acccttctac tttatcgcgc
     6361 tccttgcctg cctcacggga caggtttaaa ggaatattta gttatcattt actatagcac
     6421 aaagcatgct taaaatcaag caaaaagttt caatgtagca tcttacaaat tgctaaaatc
     6481 atataattgt gggtactgat cacactctgg attttttgga tggcaaatgg ctcttccaaa
     6541 ataaatcatg gcctgatggg cagctaacca ctgctcaggc ggcaagatat ccatgacccg
     6601 cttttccacc tcaagtggcg tcgctgattt tttgacaata tcgtggtgtt tgcaaatacg
     6661 ctccacatga gtatccactg caaaggctgg aatcccaaat cctacactca tgacaacatt
     6721 ggctgtcttg cgaccaacac ctgccaaact ctccaattct tcacgtgtct gagggacttg
     6781 accatcaaaa tcgtctagta actgttgggc acatttttta aggaatttag ctttattccg
     6841 atacaatccc aagcgagaaa tatgtgaagc aatctcactc tctgtcgcta cagacatagc
     6901 ttggggtgtt ggaaaggcaa caaagagacc tggtgtggcc ttatttaccg ctgcatctgt
     6961 cgtctgggct gataacatga ccgcaaccag gagttcaaaa tgattggtaa aatcaagact
     7021 aggcttggca tctgggaaga gggcaatgat ttcttctagc acctttcgtg ctcgtttttt
     7081 tgacaagacc attattcatc tccgtcaaat agtccttgta agctagcaaa aggactgttt
     7141 tcttctttct ttactgcttg ttgagcttgg tattcttcct ctgtcatgat ttgccagtca
     7201 tttcctgaga caaatccttg accagcttct tcttcagccg tcaagacctt gataggaatg
     7261 tttagcagga tattgtctga tacactctca gcaaggtcaa gcgccccatt ttcgatgggc
     7321 aagaccaagt catcatctaa aatttcttga tttagatggt tagttgcgcc ttccatgaaa
     7381 acttccgtga ctggataaga ttcaactaac tcaactggct ccatactgcg actcgaagca
     7441 agaacaatgg tataagatag ttgataatct aagaaataca tacggtcttc atattgtact
     7501 ttcccaactg caaggatatc ttttacatct aaaatttctt gattacgtgc acgcaggtca
     7561 tcaactaaat ctaacgtttg ttcaaagttc aaaccttcag actgcttacg aatttcttga
     7621 atatttaatt tcatacttcc tccataaaga tttactctct tgattatacc atgaaaaggc
     7681 tacaaatcag cacaccaaac tttgtaatta aaattcaaaa ttttaacata tttaccatga
     7741 tagttttgtt ttttagtgct atactatagg gaaagagtac atcagatcaa ggaggatgct
     7801 cacatggaag acaagaaact cattcaactc ctatccaagt taaataaaag ctaccaaaac
     7861 tgtaaacagg gtacggcaga tgatattcga ctacaagagc tgctaaacac tactatgcaa
     7921 gagctcaaaa aaacggaaaa gttgaacaac agtatcttaa ttgatcttga gaaattttac
     7981 caacccacca gtcctctgat tggactgggt agcctaaaac taaacaatca agcacgcact
     8041 gcttggcgaa actatgataa aatccattac gagcatgtca aacacgtact aagtctctat
     8101 ggacctgttt ttggatttta gagcatagaa tttccagttt tctgttgaca aaatttcctt
     8161 aaaggtatag tataaagata ctaatactcg gaggtaaggg agacatgaac aactaagtct
     8221 atcaaataaa gaacctttat ttagtagatc ttgtttttgt ctctttttgt gtgctctttt
     8281 atgctctttt tctggcatgt taatagagtt tttttgacat agactttggg ctctactagg
     8341 taaagtagag ctttttgtta tgcactatga acattctaga aagggaaatc atatgataaa
     8401 aatcaatcat ctaaccatca cacaaaacaa agatttacga gatcttgtat ctgacctaac
     8461 catgaccatc caagacgggg aaaaggttgc tattattggt gaagaaggaa atggcaaatc
     8521 aaccttactt aaaattttaa tgggggaagc tttgtctgat ttcactatca agggaaacat
     8581 ccaatctgac tatcagtcac tggcctacat tcctcaaaaa gtccctgagg acctaaaaaa
     8641 gaaaacttta cacgactact tctttttaga ttctattgat ttagactaca gtatcctcta
     8701 tcgtttggcg gaggaattgc attttgatag caatcgtttc gcaagtgacc aagagattgg
     8761 caatctatca gggggcgaag ctttgaaaat tcagcttatc catgagttag ccaaaccctt
     8821 tgagattcta tttttagatg aaccttcaaa tgacctagac cttgagacag ttgattggct
     8881 aaaaggccag attcaaaaga ccaggcaaac cgttattttc atttcccatg atgaagactt
     8941 tctttctgaa acggcagaca ctattgttca cttgcgactg gtcaaacacc gtaaagaagc
     9001 ggaaacgcta gtagagcatt tagactatga tagctatagt gagcagagaa aggctaattt
     9061 tgccaaacaa agtcagcaag ctgctaacaa ccaaagagcc tacgataaaa ccatggaaaa
     9121 acatcggaga gttaagcaaa atgtagaaac tgcgcttcga gctaccaaag atagtactgc
     9181 cggtcgccta ttggctaaaa agatgaaaac tgtcctctca caagaaaaac gctacgaaaa
     9241 ggcagctcag tccatgactc aaaagccact tgaagaggaa caaatccaac ttttcttttc
     9301 agacatccaa ccattaccag cttctaaagt cttagtccaa ctggaaaaag aaaatttgtc
     9361 cattgacgac cgagttttgg ttcaaaaact acaactaact gtccgtggcc aagaaaaaat
     9421 cggtattatc gggccaaatg gtgttgggaa atcaactctg ttagccaagt tacagagact
     9481 tctgaatgat aaaagagaga tttcacttgg ttttatgcca caagattacc acaaaaaact
     9541 gcaattggat ttatccccaa tagcctatct cagtaaaact ggggaaaaag aggaactaca
     9601 gaaaatccaa tctcacctag ctagtctcaa tttcagttat ccagaaatgc agcatcaaat
     9661 tcgctcctta tctggcggac aacagggaaa actcctgctt ttggatttag tcctgcgcaa
     9721 accaaacttt ctcctgctgg atgaacccac acgaaacttt tctcccactt ctcaacccca
     9781 aatcagaaaa ctctttgcta cctatccagg cggtctcatc actgtttcgc atgaccgtcg
     9841 tttcttaaaa gaagtctgct cgatcatcta tcgcatgaca gaacacggtt tgaagctagt
     9901 taatttagaa gatttataaa tttgcaacat agcaaaaatc cagagacgac ctctggattc
     9961 ttttacatct gttttaaacg ttcaatccgt tctgagatag gtgggtgggt ataaaagagt
    10021 ttttggaacc ccccaccttt cttaggatca ttgatataaa gggcactgct agcatcatcg
    10081 acgtggcgac tcataggttt gctattgtcc aacttatcta gggcattaat cattccctgg
    10141 ggattgcgag tcagctcgac actagatgca tctgccagaa attccctctg acgagaaata
    10201 gcgagctgaa ccaaggttgc agcgagaggt gccagtacaa tagctagtag ggaaaccact
    10261 agcataatga tttcaagacc atttccatct cggtcatcat cacttcgtct gcgacctgct
    10321 ccaccccacc acatcatacg acctgccata ctagaaagca tggtgatagc actagcaagg
    10381 gcaactgcaa tagtcgaaat acggatatca taattacgaa tatgactgac ttcatgtccc
    10441 ataacagctt ctagttcttc acgattcatg atagctagta gacctgaagt cgcagcaaca
    10501 gccgcatttt gaggattaga acctgtcgca aaggcattta aggctggatc atcaatgatg
    10561 aaaacacggg gcataggaat ctgagcgacc agagccatat cttccactac atggtagagg
    10621 tctggtgccg tttgctcatc cacctcacgc gctccattca tggacatgac aatctctgtc
    10681 gattgaaaaa tcatagacaa agcgtagata aagccgataa tcagtgcaat aaccaaacca
    10741 ccaagtccag atcttataaa gagataacca accgcataac caacaagagc taagagtagg
    10801 aaaaatacca gcaacaaaat ccaggttttt cgtttattgc ttgcaatttg atcaaacaac
    10861 atcttagtca cctaaaccgc taaaatcaac tttaggaacc gacttttcct cttcaggtgt
    10921 ttgaaggaaa tctgccgctt taaatccaaa cattccagcg ataatattgc tcgggaaagt
    10981 ttctaatttt acattgtagt tgctgacaac actgttatag agttgacgag agtaagaaat
    11041 tttattttct gtgtttgtca actcctcttg caatttaaca aagttagcac tagctttcaa
    11101 atctggatag ctttctgcaa ctgcaaaaat acctgaaacc tgacgagtga gggcatcact
    11161 ggctttcata gcttctgctg gtgaagtcgc tgccgccact tggttacgta gttctgccac
    11221 cttttcaagg gtagaacctt catatttggc ataacctttt acagtctcaa tcaagtttgg
    11281 caagaggtca tttcgacgtt tcaactgaac atcaatctga ctccaagcct ccttggtttg
    11341 catacgattt ttaaccaaac cgttatagct aacaatcaca aaaataacaa taagagcgat
    11401 aactccaaga ataatccaag tcataatata agtcctttct gcttttagat tagtaccagt
    11461 atatcaaatt ttctatgatt gtggtaaaat aagatgatac taaagaagga aataactatg
    11521 aaaccaaaaa cattttacaa cttgcttgcc gagcagaatc ttccactttc ggaccagcaa
    11581 aaagaacaat ttgaacgtta ttttgagctc ttggtcgagt ggaatgagaa gattaatttg
    11641 acggcgatta cggacaagga agaagtttat ctcaaacatt tttacgattc gattgcaccc
    11701 attcttcaag gtttgattcc caatgaaact atcaaacttc ttgatatcgg ggctggggca
    11761 ggatttccta gtctaccaat gaaaattctc tatccggagt tagatgtgac cattattgat
    11821 tcactcaata agcgcatcaa cttcctacaa ctcttggctc aagaactgga tttgaacgga
    11881 gttcatttct accacggacg tgccgaagat tttgcccaag acaagaactt ccgtgctcaa
    11941 tatgattttg taacagctcg tgcggttgcc cgtatgcagg tcctatctga attgaccatt
    12001 ccctacctta aggttggtgg aaaactattg gcactcaagg ccagcaatgc gcctgaggaa
    12061 ttattagaag ctaagaatgc cctcaatctc ctttttagta aggtcgaaga caatctcagc
    12121 tacgccctac cgaatagaga tccgcgctat atcacagtgg tagaaaagaa aaaagaaaca
    12181 ccaaataaat atccgcgtaa ggctggtatg ccaaataaac gcccacttta aattttttag
    12241 taaacaaatg tttacaaaat cagcctcgct cttttatttc taggctcggg aaaaaatgat
    12301 ttacaaaatc agcctcgctc ttttatttct aggctcggga aaaaatgatt tacaaaatca
    12361 tttttttctg ctatactatc ctaagcaaag gtttttaatg tcatcccgtg aggtgacgaa
    12421 gacgcagaaa tatttgaaac tctttaaaat ctaaatttta aagaagtctt actctgaggg
    12481 cctattgctg taaaataatg ggctcttttt tgatgcccaa aagtgaggtt tatatgaaac
    12541 aagaatcaac tgttgatttg ttactagacg ttgaccaacg tccttcggat ggaaaaggaa
    12601 ttctccttag cttccaacac gttttcgcca tgtttggtgc gaccatcttg gtaccattga
    12661 ttttgggaat gcctgtatct gttgcccttt ttgcttcagg tgttggaaca ctcatctaca
    12721 tgattgctac tggttttaaa gttccagttt atctaggttc ttcatttgcc tttatcacag
    12781 ctatgtcact ggctatgaaa gaaatggggg gggatgtatc tgctgcccaa acaggggtta
    12841 tcttgactgg tttggtctat gtccttgttg ctaccagcat ccgatttgta ggaacaaaat
    12901 ggattgataa actcttgcca ccaatcatta tcggtcctat gatcatcgtt atcggtcttg
    12961 gacttgcagg ttcagctgtt accaatgcag gtcttgtagc agacggaaat tggaaaaatg
    13021 ctctggtagc cgttgttact ttcctaattg ctgcctttat caatacaaaa ggaaaaggct
    13081 tcctacgaat cattccattc ctctttgcca ttatcggtgg ttaccttttc gcactaactc
    13141 ttggcttggt tgactttaca ccagttctta aagccaactg gttcgaaatt cctggtttct
    13201 acttgccatt tagcacaggt ggtgccttta aagagtacaa tctttacttt ggtccagaag
    13261 ccatcgctat cttgccaatc gctatcgtaa caatttctga acatatcgga gaccatactg
    13321 ttttgggtca aatctgtggt cgtcaattct taaaagaacc aggtcttcac cgtactcttc
    13381 ttggtgacgg tatcgcaact tctgtttctg ccttccttgg tggaccagcc aatacaactt
    13441 acggagaaaa tacaggggtt atcggtatga ctcgtatcgc ttctgtctca gttatccgta
    13501 acgctgcctt catcgcgatt gccctcagct tccttggtaa attcactgcc ttgatttcaa
    13561 ctattccaaa cgctgtactt ggtggtatgt caatccttct ctatggggtt atcgccagca
    13621 atggtttgaa agtcttgatt aaagaacgtg ttgatttcgc tcaaatgcga aacctcatca
    13681 tcgcaagtgc tatgttggtt cttggacttg gaggagctat ccttaaactt ggtccagtta
    13741 cactttcagg tactgccctt tcagccatga caggaatcat cttgaacttg atcttgccat
    13801 acgaaaataa agactaagag tctaaataca cctaatccac tcagacagct gagtggattt
    13861 ttcgtatatc ataataaaag tgtcttaaca aaattattaa aatcaaaaaa cgtataatat
    13921 cagatattct aaaaccttga tactgtacgt tttatcatag aaatttttac tttattttct
    13981 catcaaatga gatttgcatc aatctcttgt cttacttgcg tttcttcttc gctttcttca
    14041 ttttgttagc catacgtttc atggactgtt tcatggcaaa ttcaccaatt ttacctttca
    14101 aaccgccacc aaacatctgg ctcatatctg gcattcctgc tcctccgaga gctgataagt
    14161 caggcatacc gccttgtccc atcattcctt caagggcaga catatccatt cctcccatat
    14221 ttggcatatt tttaggaagg ttatttggat taatccccat ttgcttcatc attttattca
    14281 tatccccaga cataacaccc tgcatgagct gtttagcctg gttaaagtcc ttgataaatt
    14341 tattgacttc gacgaatgta tttccagaac cagcagcaat acgacggcga cggcttggat
    14401 ttaacaaatc tgggttttca cgttcttcag gtgtcatcga agacacaatg gcacgtttac
    14461 gagcaatctg gcgttcatcc accttcatgt tttgaagtgc tggattgttg gccatacctg
    14521 gaatcatctt gagcaagtct tccatcggcc ccatattttg cacctgatct aattgatcga
    14581 tgaaatcatt aaaatcaaag gtgttttcgc gcatcttctc agccatttca agggcttttt
    14641 gttcatcgta ttcctgagaa gctttctcaa tcaaagtgag catatccccc atgccaagga
    14701 tacggctaga catacggtct gggtggaagg tttcgatatc tgtaattttt tcacctgtac
    14761 cagtgaactt gattggtttt ccagtgatgt gacgaaccga tagagccgca ccaccacgag
    14821 tatcgccatc aatcttggta aggatgaccc cagtcacttc caactgagca ttaaactcac
    14881 gcgcaacatt ggctgcttcc tgaccaatca tggcatcaac gacgaggaga atttcatttg
    14941 gttgagccaa tgctttcaca tcacgaagct cattcatgag gagctcatca atttgcaaac
    15001 gacccgcagt atcaatcaag acatagtcgt tatgattagt ttgggcttgc tccaaacctt
    15061 gacgtacaat ctcaacagct ggtacttctg ttccaagtgc aaagacaggc acatcaatct
    15121 gttgtcccaa ggtcttaagc tggtcaatgg cagctggacg ataaatatcc gccgcaatca
    15181 tcaaaggacg agcattttct tctttcttga gtttgttggc caatttacca gcaaaggttg
    15241 ttttaccagc cccttgtaaa ccaaccatca tgatgatggt tggaatctta ggtgacttga
    15301 taatttctgc cgtatcagaa cctaaaacgg ctgtcagttc ctcatcaacg attttaataa
    15361 tctgttgcgc aggattaagt gtatcaatga cctcatgccc gactgcacgc tcacgaactt
    15421 tcttgataaa gtcctttaca acaggcaaag caacgtcggc ctcaagcaag gccaagcgaa
    15481 tttctttggt tgcctcttgg acatcagact ctgagatttt tcctttttta cggagatttt
    15541 taaagacgtt ctgcaaacgt tctgttaaac tttcaaatgc catgtttctt cctcttattc
    15601 tctattatca atgcttgtta aaatttctat ctgctcctgc agaaaatcat ccttgggata
    15661 gcgctccaag atttggtcaa aaatctgact acggacaatg tagtccgagt acatgtgcaa
    15721 tttcatctca taatcttcca gaatcttttc tgttcgcttg atattgtcat agacagcctg
    15781 acgactaaca ccaaactcct cagctatctc agcaagactg taatcatcag cgtagtaaag
    15841 ctctatataa ttcatttgct tatctgtcaa aagcgccgca taaaattcaa agagcgcatt
    15901 catacgattg gttttttcga tttccataac ttttattata ccaaaaaata gcctaatcta
    15961 ccacactagg gagccaatcc ttgaagatag aaagtagatt tgagaaaaac gagatcctag
    16021 ccccaagtaa tttccaattg atagctggca aagggatgcc cctcttgatt ttgtagttga
    16081 taagctagct caatcttttg cccatctact tgataacggc tcgtttgaat gataaattcc
    16141 tgcatgccca taggggtagg aatataggcc aaactatcac tatcctttaa aaagcgcata
    16201 atggtcttgg gattggaaaa tcggctcatc accagttctt gaccatgaaa tttaataact
    16261 actttttcct tttcctcatt atagaaaagc aggtagctat aatctccttt ttcatgcact
    16321 tccacatcat aaagctggtc aatcacttcc aactgctcat caaactgaat tgtatttcgc
    16381 atccgaatct tcacatcagg tcctctttct tgtctcttgt cctactattt taccaaaaag
    16441 agcaggattt tgctataatg gtcatatgaa cgaaaaagta ttccgtgacc cagttcacaa
    16501 ctacatccat gtcaataatc aaatcatcta tgacttgatt aatacaaaag aatttcagcg
    16561 tttgcgccgg atcaaacaac tgggaacttc cagttatacc ttccacggtg gagaacacag
    16621 tcgcttctct cactgtctag gagtctatga aattgcacga cgcatcacag agattttcga
    16681 agaaaaatat cctgaggaat ggaatcctgc cgagtctctc ttgaccatga ccgctgctct
    16741 cctacacgac cttgggcatg gtgcctactc ccatactttt gaaaatctct ttgatacaga
    16801 ccatgaagcc attactcagg agattattca aaatcctgag acagagattc accaagtcct
    16861 gctacaagta gcgcccgatt ttccagagaa agttgccagt gtcatagacc atacctatcc
    16921 taacaagcag gtcgtacaac tcatttctag tcagattgac gcagatcgca tggactatct
    16981 cttgcgcgac tcctatttta caggagcatc ctatggggaa tttgacctga ctcgcatcct
    17041 ccgagtcatt cgtcctgtcg aaaatggtat cgcctttcag cgcaatggca tgcacgccat
    17101 cgaagactac gtcctcagtc gctaccagat gtacatgcag gtttatttcc acccagcaac
    17161 acgcgccatg gaagttctcc tacagaatct tctcaagcgc gccaaggaac tctatcctga
    17221 ggacaaggat ttctttgccc gaacttctcc acacctcctg cctttcttcg aaaaaaatgt
    17281 gaccttgact gactatctgg ctctggatga tggcgtgatg aatacctact tccagctttg
    17341 gatgaccagt cctgacaaga ttcttgcaga tttatcgcat cgctttgtca accgcaaggt
    17401 ctttaaatcc attacctttt cacaagagga ccaagatcaa cttactagca tgagaaaatt
    17461 ggttgaggat atcggctttg atcccgacta ctacactgcc attcataaga actttgacct
    17521 cccttatgat atctatcgtc ccgaatctga aaacccacgg acacagattg agattttaca
    17581 aaaaaatgga gaactggccg aactctctag cctgtctctt atcgtccaat cccttgctgg
    17641 cagtcgccac ggagataatc gtttctattt ccctaaagaa atgttagacc aaaacagcat
    17701 cttcgcaagc attacccagc aatttttaca cttgattgag aacgatcatt ttaccccaaa
    17761 taaaaactag aagaggaatt ttatgagtat taaactaatt gccgtcgata ttgacggaac
    17821 cctagtcaac agccaaaagg aaatcactcc tgaagtcttt tctgccatcc aagatgccaa
    17881 agaagctggt gtcaaagtcg tgattgcaac tggccgcccc atcgcaggtg ttgccaaact
    17941 tctggacgac ttgcagttga gagaccaagg tgactatgtg gtgaccttca atggtgccct
    18001 tgtccaagaa actgctactg gccatgagat tatcagcgaa tccttgacct atgaggatta
    18061 tctggatatg gaattcctca gtcgcaagct cggtgtccac atgcatgcca ttaccaagga
    18121 cggtatctat actgcaaatc gcaatatcgg aaaatacact gtacacgaat caaccctcgt
    18181 cagcatgcct atcttctacc gtacccctga agaaatggct ggcaaagaaa ttgttaaatg
    18241 tatgtttatc gatgaaccag aaattctcga tgctgcgatt gaaaaaattc cagcagaatt
    18301 ttacgagcgc tactccatca acaaatctgc tcctttctac ctcgaactcc ttaaaaagaa
    18361 tgtagacaag ggttcagcca ttactcactt ggctgaaaaa ctcggattga ccaaagatga
    18421 aaccatggca atcggtgatg aagaaaatga ccgtgccatg ctggaagtcg ttggaaaccc
    18481 cgttgtcatg gaaaatggaa atccagaaat caaaaaaatc gccaaataca tcaccaaaac
    18541 aaatgacgaa tccggcgttg cccatgccat ccgaacatgg gtactgtaaa agtatcattt
    18601 ttcaataaga attgattagc aataaaatcc aatgaatttt tttagcaaac tatttaattt
    18661 aaaacaaaat aatcataata gagacacaaa ttctgattgt aacaattttt acctaaacga
    18721 attagaatgt ggccttactc ctgggcaact catactcata gattggactc aaaaaacagg
    18781 gagaaattat aatttcccaa gatattttaa atactctctt caaattgacc ctgaatctac
    18841 acacaatcaa ttatacaaat taggatactt cactaaaaat aagactttat catatcttac
    18901 agtagtagaa ttaaaaacta tattatctaa acataattta gctacttctg gaaaaaaagc
    18961 agaattaatt acaagaataa ttaataatgt taacattgac aatttagata ttccgttcga
    19021 atttaaacta acaaaagaag cacaaaatct tattatcgaa catagtgact atatcaaagc
    19081 atactatgat aaagacataa ctatggaaga ttattgtaaa gaaaaaaaca atatctcttt
    19141 taaagcaact tttggtgata taaaatggag tctcttaaat aaacaagctc ataggaatac
    19201 tgtatcagga gattttggat gcttatctaa cacacgaaag gctcagggaa gacatttgga
    19261 acaagaaggt aatattaaac atgctttaat atattacata gaatctttga taattactat
    19321 ttcaggatta gaaaacaatt tttcagccac tgattatcca gtatattatc ccgattcgat
    19381 acctgactac tcactaaaac atattcaaac attaatggaa tcattatctg atgacgatta
    19441 tgattttgct tttgatgaag cattatttcg cttctcaatt ttgaatgcaa atcatttttt
    19501 atctaaggaa gatattgact atttaagagt taatttacct cgttccactg ctgaagaaat
    19561 aaacaattac ttaaagaaat atgaatgtta tagtccttta aataatttag aacttgacga
    19621 ttttgaataa attgactata caaacattta tatactcgat atagtctcaa ttttatctga
    19681 tgattgccca aatttttcaa taataaaacg cataatatta tggagacaat cccctatatt
    19741 atgcgttctt ttaatatcaa agactttttg acaaacttct ttgatatcta attacatgcc
    19801 ccctgcagga atcgaacctg caactactcc ttaggaggga gttgttatat ccattgaact
    19861 aagggagcta gataaaaact ctgctgaaaa gcagagtttt ttagtcgaat taacgacgga
    19921 tttctttgat acgagctgct ttaccttgaa gagcacgcaa gtagtacaat ttcgcacgac
    19981 gtactttacc gtaacgaaca acttcgattt tttcaacacg tggagtgtgg attgggaaga
    20041 tacgctcaac acctacaccg ttagagattt tacgaactgt gtagttttct gagattccag
    20101 cacctttacg tgcgataaca acaccttcaa aaatctggat acgttcacgg ttaccttcga
    20161 caactttcgc gtgtacacga acagtgtcac caggacggaa tgatgggata tctgtacgaa
    20221 gttgaccttc agtcaagctt tggattaatg gattcatttt attctcctat ctttgtcaat
    20281 cttgaggaaa cttcctcagc ggataaactg tattcttgtg cgtccattac acacaagata
    20341 cagtttacca aattttcaca aaaaagtaaa gaaatttata gcaaaattcc taaaaaaatc
    20401 gcaaccaaac cacctatgta agtcaaagtc aggtaagaat aaaagacctt cttatcactt
    20461 agcagtcttt gaagttcgtc attcaaggtc gaaaaagttg ttaaaccgcc acaaaatcct
    20521 gttgctagaa tagcatatac ttccttagac tctacatgat tgtagaatac tccaattaaa
    20581 aaacaaccta aaagattagc tatgagcgtt ccgagtggca atttagaagc ttgattatag
    20641 cgagaaaaga aataccgtac gagtgctccg aaaccacaag caattgcaag atagacaatt
    20701 accatttctt cctccctaga aaataagcta agagtaggcc tccaccgatg ctcaaaagca
    20761 aatacaggac taaactaaaa taacgccctg tatcaagcag cttcacagta tcaagcatta
    20821 ggctggaaaa agttgttaaa cctccgcaaa aacccgtccc cagtgctaaa atcaagccct
    20881 tactagtccc cttatagacc agatagccct tgacaacaaa aatcaagcag aaaattccca
    20941 gatagttgac aaagagagtg ccccaaggaa agtctggact ggctggtaac caggtggaaa
    21001 ctagatatcg gacaagtccg cccaacatag cagctagaaa aattcctagc ggataaaatt
    21061 gttctttttt catttgatat tttgatcctg ataatcacgc gaacgtttga gtatgtccga
    21121 aaaagtcgct acaatagtct cctgatagcg cttgtcctct acacgactcc taaccttttc
    21181 aaaaataact tcttctctct tagtatctaa aatcggttta cctgaagctt tcttataagc
    21241 aaccaccccc tcaactaaat gcattcgttc ttctaacagt ttgacaattt ggtcatcgat
    21301 ttgatcgatt tcttgacgaa taatatctaa atccatacag tctcctcctt tatttgaatt
    21361 attgtatcaa aaagccatca attaggctag taaaatccca actctcgata agaaaaatgc
    21421 actgattgat tacatcagcg cacttttatg cttatactac tttagcaacc aattcttctg
    21481 cgaattgttc caagcgttca atatcttctt cctcggcaga aagatcaact ttgacacact
    21541 cagaaccttt ttctgctcct gttgacacaa agatacggtc aaagtcatca acagccttac
    21601 agaattcatc gtagaaggta tctcctgagc cgaccactcc gtagattttg ccattcaagt
    21661 tgagatctgc taggtcttcg tagaagtcca tcatctcatc tggcaattca ccatcaccat
    21721 aagtataggt cgcaacgata gcgatgtctg cttccaagaa gtctgaagcg tcaacagttg
    21781 tacattcatc aacatcaaca tccaagccca agtcacgtaa tttgtctgct acaatatctg
    21841 caatttcttc ggtattaccg gtcatactgg caaatacaat ttttgctaat gccatatcgt
    21901 cctcctcaat ttatctttct ccattatatc acatcttttt cattttaaaa ataaaaattc
    21961 ttgtgctaca atgaaatgag aaagaattga ggttatttat ggagatttgc caacaaattt
    22021 tagaaaaaat taaagaatac gacaccatta tcattcatcg tcatatgaaa ccagaccctg
    22081 atgccttggg aagtcaggtg ggattgaaag ccttgctgga acatcatttc ccagaaaaaa
    22141 ccatcaaagc cgtcggtttt gatgaaccaa ctcttacttg gatggctgag atggatcttg
    22201 ttgaagatag agcctaccaa ggcgcacttg tcatcgtctg tgatacagct aatactgctc
    22261 gtatcgatga taagcgctat agtcaaggtg attttctcat taagattgac caccatccaa
    22321 atgatgatgt atacggtgac ctgtcttggg tcgatactag ttcaagtagc gctagcgaga
    22381 tgattaccct atttgcccaa acaacccaac tagccttggc agatcgcgat gctgagttgc
    22441 tctttgcagg aattgtcggt gatacaggtc gcttcctcta cccttctacc actgcacgga
    22501 ctcttcgcct ggctgcttat ttgagagaac ataactttga ctttgcggct ctcactcgca
    22561 aaatggacac tatgagctac aaaattgcta aactgcaagg ctacatctac gaccatctgg
    22621 aagtggatga aaatggtgct gctcgcgtta tcctgagtca agaagtcttg aaacaataca
    22681 atgtaaccga tgctgaaact gcagccattg tcggtgcacc tggacgcatt gacagagtga
    22741 gtctctgggg aatttttgtc gaacaggctg atggccacta ccgagttcgc ttacgcagta
    22801 aaatccatcc tatcaatgaa atcgccaagg agcatgatgg tggaggccac cctctagcaa
    22861 gtggtgctaa ttcctatagc ctagaagaaa acgaaatcat ctaccaaaag ttaaaaaact
    22921 tgcttaaaaa ctgataaaat acttgccaaa cttttcagaa tctgatagac tagtatggta
    22981 acaatctatg gctcgcaaag agaccatggc agaaaggaaa tattgcaaaa tgaaaaaaga
    23041 tatccatcca gaatatcgcc cagttgtctt catggacaca actactggtt accaattcct
    23101 tagcggttca acaaaacgct ctaacgaaac agttgagttc gaaggcgaaa cttacccatt
    23161 gatccgtgtg gaaatttcat cagactcaca cccattctac actggacgtc aaaagttcac
    23221 tcaagcagat ggacgcgtgg atcgtttcaa caaaaaatac ggtctcaaat aatgataaaa
    23281 gaacagtttc ggctgttctt ttttgtttct tgaaatcaac tgctgttttc atgttccaga
    23341 ctcatctgta ggttcggttt ccatgctact aggcaggaag gaaatagctg tttcaacacg
    23401 tccataatga gctgtactat tgtcacgaac cacactttca ttgatggtcc aagtggaatt
    23461 cattttctta aaagcttctc ggactttttc caaatctttg gaggcaatgg cctgctctaa
    23521 ggtttcaaaa cgaggactta gacggtttac caatttttcc ttttccgcat ctaggtcaac
    23581 aggattttgt tctttttcaa aagctaatag agctgaggaa atttcggtga gtttggcttc
    23641 agtaatatct ccagatagag ctaacttttc cttaacaatc ttaccaacct cagaatcctt
    23701 attttctact ctttcgaagt ctgatgccat cttcgtaacg agtttctggg tctcagtctg
    23761 accaccattt ttaaccgctt tggatgcatc tgtgattttc acaaagaaaa aactcaagct
    23821 ttcttgggca cctgctgggg aaagaattaa aaatgacaaa gctaaaacta gtaaccagct
    23881 tttagccttc caataatttc tgaccaatgt agcctccttt ctccacacca ccaaagcaag
    23941 taaaaagtcc actacctatg tgagtgatgt attcattcat cttatcagta gcacctaggt
    24001 tagtttgaac cttaacaaag ttatcaggat ccttctggaa agaaatgaag agcaatcccg
    24061 tatcaaactg accagtcttt tcatcaatgc catctgagta agagtaagaa caacgcaaaa
    24121 gtggcttgtc cacttactta gccaaacgaa catgcgaatc atctagcaaa aaactcaaat
    24181 tcacttcatc aaactcattt ttttaccgaa aggggcacta ctttccttgt aacgaccaaa
    24241 ggtattttct tgttcttcga gaccagtgcg gtcccaggtc tctaaaaaca tctgaatccg
    24301 acgaactgcc atataagaac cattttccat ccagtcctta ctatcagccc agataacgcg
    24361 gtcaaagtct tgctccttgg ttgaatttgc tgttccatcc ttaaaaccaa agaggttcca
    24421 cggagtctcc attcgtcccc gatagctgca aatccagact gactccaacg aaggggcacc
    24481 gcatttctcc ccttacgtat gagattgcga ataacgtgaa aggcaatttg ctcatcatct
    24541 gcacaagcat gaatcacaat atctccacca gtatattttt cacgtaattg ctccttagga
    24601 aatagaggta aatccctgaa cagtcgaggg cgcttgtttt ccaagttcat ctttttcaag
    24661 aaacttgcag atacaccaaa agtcagcgtc aaacgatgag gattgagccc aactgtctca
    24721 ccagtatcgc tcggaggaaa gagagcattc tgtccatctt ttttaaccaa ttccccctcg
    24781 accaacttgg cactataatc cgtccaatcc ttgaatagct ggataatttt atctctatcc
    24841 gtcgtatgca ggtccaagac aacaaaatag atattctttt gcatgggcgt actatttggt
    24901 acatcaagcg aaccttacga aaacgtctac tatatttaaa ccgctttctt gtcttcttga
    24961 attgagattt tatttttaga cgcgacaatg gtttgagcgt ctgctttctt aggatgttct
    25021 ggacgtggca taatatttcg ccaaccatgg cgcttcaaca g
//
LOCUS       DBEROU010000032        24762 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.032, whole genome shotgun sequence.
ACCESSION   DBEROU010000032 DBEROU010000000
VERSION     DBEROU010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.032"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..307)
                     /locus_tag="ACL8CL_001422"
                     /pseudo
     CDS             complement(1..307)
                     /locus_tag="ACL8CL_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            534..854
                     /locus_tag="ACL8CL_001423"
                     /pseudo
     CDS             534..854
                     /locus_tag="ACL8CL_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884744.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
     gene            975..1919
                     /gene="manA"
                     /locus_tag="ACL8CL_001424"
     CDS             975..1919
                     /gene="manA"
                     /locus_tag="ACL8CL_001424"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HGS1330657.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVEAWTLEGQDLELIVSHP"
     gene            complement(1910..3305)
                     /locus_tag="ACL8CL_001425"
                     /pseudo
     CDS             complement(1910..3305)
                     /locus_tag="ACL8CL_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844893.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            3511..4256
                     /locus_tag="ACL8CL_001426"
                     /pseudo
     CDS             3511..4256
                     /locus_tag="ACL8CL_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274781.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
     gene            complement(4285..4740)
                     /locus_tag="ACL8CL_001427"
     CDS             complement(4285..4740)
                     /locus_tag="ACL8CL_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HGS1330658.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(4760..5935)
                     /locus_tag="ACL8CL_001428"
     CDS             complement(4760..5935)
                     /locus_tag="ACL8CL_001428"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HGS1330659.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELDGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(5994..6839)
                     /locus_tag="ACL8CL_001429"
     CDS             complement(5994..6839)
                     /locus_tag="ACL8CL_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS1330660.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEEGGLLM
                     GYEID"
     gene            6964..7521
                     /locus_tag="ACL8CL_001430"
     CDS             6964..7521
                     /locus_tag="ACL8CL_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HGS1330661.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            7540..8007
                     /locus_tag="ACL8CL_001431"
     CDS             7540..8007
                     /locus_tag="ACL8CL_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HGS1330662.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            8096..8725
                     /gene="upp"
                     /locus_tag="ACL8CL_001432"
     CDS             8096..8725
                     /gene="upp"
                     /locus_tag="ACL8CL_001432"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HGS1330663.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            8899..9489
                     /gene="clpP"
                     /locus_tag="ACL8CL_001433"
     CDS             8899..9489
                     /gene="clpP"
                     /locus_tag="ACL8CL_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HGS1330664.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSAQETLEYGFIDEIMANNSLN"
     gene            9568..9816
                     /locus_tag="ACL8CL_001434"
     CDS             9568..9816
                     /locus_tag="ACL8CL_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HGS1330665.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQELETPFVGNLYR"
     gene            9917..11077
                     /locus_tag="ACL8CL_001435"
     CDS             9917..11077
                     /locus_tag="ACL8CL_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HGS1330666.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKDNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            11345..12214
                     /locus_tag="ACL8CL_001436"
     CDS             11345..12214
                     /locus_tag="ACL8CL_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGS1330667.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLATAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            12218..13174
                     /locus_tag="ACL8CL_001437"
     CDS             12218..13174
                     /locus_tag="ACL8CL_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HGS1330668.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSTSPTYGAFFGAMLVGALLSG
                     VVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            13174..13938
                     /locus_tag="ACL8CL_001438"
     CDS             13174..13938
                     /locus_tag="ACL8CL_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330669.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            13938..14648
                     /locus_tag="ACL8CL_001439"
     CDS             13938..14648
                     /locus_tag="ACL8CL_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330670.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKIPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     TGKELASSEEVRKAYLGG"
     gene            14956..15612
                     /locus_tag="ACL8CL_001440"
     CDS             14956..15612
                     /locus_tag="ACL8CL_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HGS1330671.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQELHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIISL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDGSIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            15720..16815
                     /gene="prfB"
                     /locus_tag="ACL8CL_001441"
     CDS             join(15720..15791,15793..16815)
                     /gene="prfB"
                     /locus_tag="ACL8CL_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HGS1330672.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTELD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            16833..17525
                     /gene="ftsE"
                     /locus_tag="ACL8CL_001442"
     CDS             16833..17525
                     /gene="ftsE"
                     /locus_tag="ACL8CL_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HGS1330673.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            17518..18444
                     /gene="ftsX"
                     /locus_tag="ACL8CL_001443"
     CDS             17518..18444
                     /gene="ftsX"
                     /locus_tag="ACL8CL_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HGS1330674.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANTPNDVKTIAEDAKKIEGV
                     SEVQDGGANTEKLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            18730..20910
                     /locus_tag="ACL8CL_001444"
     CDS             18730..20910
                     /locus_tag="ACL8CL_001444"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIBC"
                     /protein_id="HGS1330675.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPIFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEA
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVTAGDLLVTADL
                     DAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            20962..21777
                     /locus_tag="ACL8CL_001445"
     CDS             20962..21777
                     /locus_tag="ACL8CL_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HGS1330676.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLLEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEAVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAILNWK"
     gene            21917..23260
                     /locus_tag="ACL8CL_001446"
     CDS             21917..23260
                     /locus_tag="ACL8CL_001446"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HGS1330677.1"
                     /translation="MSFTKFQFKNYIREALKELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLASNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            23475..24665
                     /gene="metK"
                     /locus_tag="ACL8CL_001447"
     CDS             23475..24665
                     /gene="metK"
                     /locus_tag="ACL8CL_001447"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HGS1330678.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAADLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
BASE COUNT         7542 a         4622 c         5408 g         7190 t
ORIGIN      
        1 aattgagatt ttatttttag acgcgacaat ggtttgagcg tctgctttct taggatgttc
       61 tggacgtggc gtaatatttc gccaaccatg gcgcttcaac agttgataga aggcatcacg
      121 tgtgtaggaa tgacctaact ccttctatag tgtattgaat ctataacagt acaccttggc
      181 tgctaaaata tttctataaa ttaatttgac ttttctgata gagttgttca catcttattt
      241 caaactacta taaaagttct ataatctctt tataagattt gcccatcaga cgaaatataa
      301 tggattgaaa ctagaatagt acacctctac ttctaaaaca ttgttagaaa tcgatttgac
      361 tgtcctgatt gatttgtcct gttcttgttt cattttacta tagaacgatt tgaaggcgtt
      421 tatgatattt agctgtacga gagtctttta aaagtgtttt gatggtttgg atttcttctt
      481 tagttgattt catatcacta ttatataatg ctttttgatt ttagtttagt attatcgtag
      541 aaaatggact cggtgctatg gatacaccgg atgagaatgg ttatgtagca gataactatc
      601 ggattactta cttagaggcc cacatcaagg ccatgcgaga tgccatttac caagacgggg
      661 ttgacttgct tggttatacg acttggggct gtatcgatct ggtttcagct ggcacagggg
      721 aaatgaataa gcgctatggc tttatctatg tagatcgaga taatgtagga aacggagctc
      781 tcaaacgtag caagaagaag tccttctatt ggtacaagga tgtcattgat agcaatggtg
      841 caagcatcgg gtagagcaca tttgtttact atttttataa aatcttctcc ctgctttata
      901 agatgtaaaa aagagttcaa ttcgaactct ttttttgcta taatgagggg agaaaaatca
      961 gacaggagat cgacatgtca gaaccattat ttttacaatc agttatgcaa gaaaaaatct
     1021 ggggtggagc caagctacgt gatgagtttg gctacgacat cccaagtgaa aaaatcggag
     1081 aatattgggc catctcagcc catccaaatg gagtctctaa agttgccaat ggtcgttacg
     1141 agggaacaga tcttgctact ttgtatgcgg aacaccgtga attatttggc aatcgtccag
     1201 aacctgtatt tccacttttg accaagatcc tcgatgccaa cgactggctc agtgtccaag
     1261 ttcacccaga cgatgcttat ggactcgagc atgaaggcga actcggaaaa acagaatgct
     1321 ggtacattat cgcagcggat gaaggttcag agattatcta cggtcacaat gccaagtcaa
     1381 aagaagaact ccgccagcaa atcgaggata agaactggga tgcgctgttg acaaaagtgc
     1441 cagttaaagc aggagatttc ttctatgtac caagcggtac catgcacgct atcggggcgg
     1501 gtatcttgat tcttgaaacc cagcagtcta gcgataccac ttaccgtgtc tatgactttg
     1561 accgtaagga tgacaagggc aatttacgtg aacttcacct tgaaaaatct atcgatgtct
     1621 tgaacattgg tgaacccgca aatagccgtc cagtgacaat caaagcagat gatctacgtt
     1681 ccactctcct tgtatccaat gatttcttcg cagtttacaa atgggaaatt actggaaaag
     1741 ttaactttga aaagacagct gactacagct tgttgagtgt cttggctggt caaggtcaac
     1801 taactgttga cgggaaaaac tatccaatcc aaaaagggag acactttatc ctaccaagtg
     1861 atgttgaagc ttggactctg gaagggcaag atttagaatt aattgttagt catccataaa
     1921 aaagaaaggg cctgagttca ttactcaagt ccttttgatt acataaattg ggcgatgaag
     1981 accacgatga tgattggaat gacggaacga agaaacgaag aaggaagagc cagacttgga
     2041 acagtccttg tttccatgct ctttcatcga gatggagttc ctccattgca agagcctttt
     2101 taaagatata gcctgtaaaa agtgaaaggt agagagctcc aaatggcatg aggagattgg
     2161 aaaccaagaa gtccatagcg tcaaagaagg tcttaccaaa aatgtgaaca tccgccatga
     2221 caccgtaaga tagggctgaa ggaatgccaa agacaaaggt caaaattcct aaaataacac
     2281 tccatttggc acgtttgctg ttatcctggt tggtgatatt gcccacattg atctccagca
     2341 tcacgacaga agaagtgaca gtcgcaaaga ggaagaggac gtagaaaatg gttccaaaag
     2401 gcatcttgtc aaagagttga ggcaagacga taaagagcaa gctcggtccc ccttcagact
     2461 ggatattgaa gggggatcga gcttggaaaa tggctagacc tgccatgatg gatatcgaga
     2521 tattcatggc tacgatggag attcctgact ggactagatt ggttttcttg tccaagtaag
     2581 gagcataggt caacatgact gtaaccccta gtgagagggc aaagaaagat tgtcccagag
     2641 catagaggag accagcgcta gtcagttttg aaaagtctgg tttgaggaag taaagaaccc
     2701 ttccatggca tttggcaaac tgagagagcg accgatgata acaacaaaga tgataaagag
     2761 caggggcatc atgactttcg aagctctttc aatccctttt tgaacaccac gtgatacaat
     2821 aaagatattt aataagataa aggctgcttg agctactagg gcaatggctg gatttgaaat
     2881 gattgaagta aataactgag cataatcacc cgttccacca agttggaaca atttcccaaa
     2941 ctcaatacct agatagacta gaatccatcc tccgataaca ctgtaaaaag ataagaggat
     3001 aaagagggca aaggcgccaa tccaaccgat aaagttgtac ttgttattct tgcccagttt
     3061 tccaaaggtt ttgatagcgg aaacgccagc cactacggcc aagggcaaac tcagccagca
     3121 ggagagggaa accgattaaa atagtggaaa tgagaaagat tagtaaaaag cctccaccgc
     3181 cattagcagc agtcatgtag ggaaacttcc aaacggaacc aagcccgatg accgagcccg
     3241 atgaccgagc cagcagatgc tagaataaag ccaagtttcg acccccattg tgatttttct
     3301 gacatagtta aactctgatc tcctttttca aaataagaaa aagctacttg agttgtcaaa
     3361 tagccttctg tttgattgat agacttgact atcctattat gttttctaag gtctgtcaag
     3421 aaaaccattt tcaagttttc acacctttct caaaaacgct tgactctgac ctaaggggag
     3481 gggttatact atcattgtaa ggaggaaatc atgtaccata taaaagaagc tgcgcagctt
     3541 tcaggtgtct ctgtcaagac cctgccctgc atcactatga caagatagga ctcttggtcc
     3601 ccttaaagtc ggaaaacggc tatcgaacct atagtcaaga ggatttggaa cgccttcagg
     3661 tcattcttta ctacaaatat ctaggctttt ctttagagaa aatagcagaa ctgttaaagg
     3721 aagaaaggac agatttattg ccccatttga ctaggcagtt ggactatcta actcgcgaaa
     3781 ggcaacatct ggataccttg atttccacct tgcaaaaaac tattcaagaa caaaaaggag
     3841 aaagaaaaat gaccattgag gaaaaattca cgggatttag ctatcaagac aatcaaaaat
     3901 accaccaaga agcggtagag aaatatggtc aagaagtcat gggacaagcg ctcgaacgcc
     3961 aaaaaggtca cgaagacgag gctacggccg cctttaacca agtctttcaa actttggcac
     4021 aaaatcttca agttggttta cctgcaacag caaccgaaaa ccaggagcaa gcagccaagc
     4081 tcttgcaagc cattcgcact tatggatttg actgctctat tgaggtattc ggtcatatcg
     4141 gtaaaggtta cgtctacaac ccagagttta aggaaaacat tgacaagttt ggttctgaaa
     4201 cagcccagta cacgtcagat gccattgcgg cttacgttca gacaaatgca gaataaatag
     4261 gctaggaatt tcctagccta ttttttactt caaatcataa agccagtcgt caccgttttt
     4321 gtagtaaaag aattcactga gatcttcttc tagaaacaca cgaagcatat cagacatatc
     4381 atcggttgca agttttagat gagaaagatt ttcaaagtcc tcccaccaaa ctttcccttc
     4441 gtctgaagac tggagttcac cagtaaagtg ttctgtcttg taaaaaagga cgacataacg
     4501 ataatccttg tcgtcatacc agtttttgat accacagagt tggggtttgg aaatgatcag
     4561 accagtttct tctttcactt cacgaatgac agcatcgaca aaggattcgc cacgttcaac
     4621 atgaccacca ggaaaagtaa tgccagacca gtcgggacta actcggtctt ggaccaggac
     4681 cttatctccg tttttaatca tacacatgtt aacaaattcg actgcctctc ttctgttcat
     4741 tcttcacaac ctttaatctt taatcataat gcagacttcc cgccacccag ccggtacaga
     4801 gggcagaagt gatgttaaag ccacccgtgt gggcattgat atccataact tcgcctgcaa
     4861 agtggaggcc aggtaccagc ttactttcaa gggttttagg attgatttcc ttgagactga
     4921 ctccaccctt ggtaacaaag gactttgcaa gggacatttt tccagttaca ggaattttaa
     4981 gttctttaat ggactggaca agttgttctc gttccttttc agtcagttgt ttgacttttt
     5041 caggatatcc ttgtacaaaa aattcggcca agcgttctgg taacaaggtt tttaaagcgt
     5101 ttttcaagga tttttcccga ttttcttcta gaaatgtaac caagtccttc tcagaaagtt
     5161 gaggcaaaac atcgagtgag agaacctccc cacctttgac aaagctagac atgcgtaggg
     5221 cagcaggacc tgacaaacca aagtgggtaa agagtaaatc atgagtgatg acatgcttac
     5281 cataacttag ggtcacatcg tccagagaaa tcccttgtaa ggctttatgt ggaaaatctg
     5341 ttaataaagg actttcagca gcctcaagat cggtgatggt atgcttaaaa tggcgagcaa
     5401 tctcgtgacc aaaaccagtc gaaccagtcg aaggataaga cttaccacct gttgtgacaa
     5461 tgagtttctc acaagtgaag gtttgatccg ctgacttaag gacaaactgg tcatctactt
     5521 ttttaacaga aacgatttct atttgagtag caacttgacc atctagttcg gtgattttct
     5581 tttccaaagc ttcgataata gtccgagact tgtcactggc tggaaagacg cgtccgtggt
     5641 cttcgacctt aagtttaaca ccattttctg taaaaaagtt gatgatgtca tgattatcga
     5701 actgggagaa aacactgtaa agaaagcgtc cgtttccagg aattccagct agcaggttgt
     5761 ctaagctacc attgttggtc acattgcaac gtcccccacc agtcccagct aatttttttc
     5821 caagtttccg atttttttcg atgaggaggg ttttctgtcc ataaaagcta ctggaaatcg
     5881 tagccatcat accagcaggt cccccaccga tgacaatagt atcaaaatgt ttcatagctc
     5941 tattgtacca caaaaaaaca agagatgatg gtcacctctt gtcaagaatg caattaatca
     6001 atttcatagc ccatcagcaa accgccctct tctgcataga aactgcagag accagaggtt
     6061 ggtagaattt taatatccgc ttgtgggaag gtttcacgga ttcgctctga gagctgttga
     6121 caacattttt cgttattgcg ttgggccatg acaatacggc caccagcata tccagctttt
     6181 actaactcat cataggcagc ttgaactgat ttctttgatc cccttgcttt ttgtagcaat
     6241 tcgagagtcc cagtttcact agcttttccg accatacgaa tgttgagaag gccaacgacc
     6301 gtaccgataa gcttgctcaa acggccgttc ttcaccaagt tatcgacttt ggctaggaca
     6361 aagagcaact tagttttttc ttgataggcg gtgatagctt caaccacttc ttcaaaagac
     6421 aagccctggt caatcaagtc attcaatttt tctacgagta ggtcaacttc accaccagca
     6481 gataaactat caatcacatg aatcttagtg tcaggatggt cttccagata aatattcttt
     6541 gctagttgag cactattgtg actgccagaa agggtacctg tgatggttac taggaaaatg
     6601 tttttggcac cttcaaatgc tcgcaaatag tcatctgggc ttggacaagc tgattttgaa
     6661 gcttctgcag ttgcatacat ggtttccatc atttggtcaa tatcgagact ggcgtcatca
     6721 acaaagacct gatcagctac ttgaatggtt aaggggacac ttacaaaggt tgtgttaata
     6781 gctggtgttg gcagttgacg ataatcacaa ccagagtcag caataatctt ccaagtcata
     6841 gaaattctcc atctttgtca gttatacatt gacaaaggtt ctgtcttttt ttacaattat
     6901 atcatgaaaa ctcttgaaac aaaagcatca tccgtctgtt gtgacaagta gaaagaaaat
     6961 gttatgtctg aacgtagaat ctctgaaaag tctcttgaaa atctcagaaa atcaaaccaa
     7021 gaatccaatt tattaaccag agaagccatt gaaacagccc tcttgcaact cttggaaaaa
     7081 aaggagctga ccaagattag tatttctgaa ttggtcaaac gtgcaggtgt ttcgcgtgcg
     7141 gccttttatc gcaattatga ctccaaagag gaaattttag agagcgtctt taaacgaact
     7201 gtccacaata ttatggaaca gctgcatcat tacgatttaa agacagacct ttatttggtt
     7261 tgggttcacc ttttccggga ggccagaaag gaagctagag taattcaatt ggccttggat
     7321 taccatctgg aaaaaatctt tgtccaagcc atgcaggaat ttctagaaaa ataccatggg
     7381 aaatcaaaag gtgtcagctc ttatcttcat tccttctgga gctcagccat cgtctctgtc
     7441 cttctaaaat ggatcaagga tggcatgaag gtaccagctg aaaagattgc agatttaggt
     7501 ttaccatttt ttaaaaaata gagaaaaagg agaaaagaga tgactgaaaa aagactggcc
     7561 tgggatgagt attttgcagc ccaagctcta ctaattgcga atcgttccac ttgtaaacgt
     7621 gctaaagtgg gcgcgattct ggtaaaagat aataaggtta tttccactgg ttacaatggt
     7681 tcggtgtcag ggactgagca ttgtattgat cacgaatgtt tggtcattga aggccactgt
     7741 gttcgcaccc ttcacgctga ggtcaatgct atccttcaag gtgctgagcg tggtgttccc
     7801 aaaggcttta cagcctatgt aacccatttt ccttgtctca actgtacaaa acaattgctt
     7861 caggtcggct gcaagcgcgt ggtttatatc aaccagtacc gaatggacga ctacgcccaa
     7921 tacctttatc aagaaaaagg gacagaattg acccatttac cacttgagac agtacagaca
     7981 gctcttaaag aggctgatct aatgtaaaaa ttatcaaaaa taaatggttt agaaagattt
     8041 ttaaaccgtt ttttggtata ataagaagaa taaattgaaa gaaggaattc caaaaatggg
     8101 aaaaattgaa gttattaatc acccactgat tcaacacaaa ttgtcaatct tgcgtcgtac
     8161 agatacttct acaaaagctt ttcgtgagct agtagatgag attgcaatgt tgatggggta
     8221 tgaagtactt cgtgatcttc cactagaaga tgtggaaatc gaaacaccaa ttacaaaaac
     8281 agttcaaaaa caattggcag gtaagaaatt ggccatcgtc ccaatcttgc gtgcaggtat
     8341 cgggatggtt gatggtctct tgagcttggt tccagctgct aaagttggcc acatcggtat
     8401 gtaccgtgat gaagaaacac ttcaaccagt tgaatacttg gtgaaattgc ctgaggacat
     8461 tgaccaacgt caaatttttg tagtagaccc aatgttggca acaggtggct cagcaatctt
     8521 ggctgttgat tctcttaaaa aacgtggcgc atcaaatatc aaatttgtct gccttgtatc
     8581 tgctccagag ggtgtaaaag cccttcaaga agctcatcca gatgtagaaa tctttacagc
     8641 agccttggat gaacgtttga acgaacacgg ttatatagtt ccaggtcttg gagatgctgg
     8701 agaccgcttg ttcggtacaa aataagatcg aaaagaagaa tgacttggaa aagtatttct
     8761 agtcacgaaa ggaggttggg tttttgtttc tgtctaatga aagcagagca aaaatttgac
     8821 cttttttgac caagatatta taatagtctt atcttcagtc atttgaccaa caaaattaaa
     8881 actcaaaagg agaaatgaat gattcctgta gttattgaac aaacaagccg tggagaacgt
     8941 tcttacgata tttactcacg tcttctcaaa gaccgcatca ttatgctgac aggtccggtt
     9001 gaagacaata tggctaactc tgttattgcc caattgcttt tcttggatgc ccaagatagt
     9061 acgaaagata tttaccttta tgtcaataca ccaggtggtt ctgtttcagc tggtttggca
     9121 atcgtagata ccatgaactt tatcaaggca gatgtccaaa ccattgttat gggaatggct
     9181 gcatctatgg ggactgtcat cgcatcaagt ggagcaaaag gcaaacgttt catgcttcca
     9241 aatgctgaat acatgattca ccaaccaatg ggcggtacag gtggtggtac ccaacaaact
     9301 gatatggcta tcgctgcaga acacttgctc aaaactcgta ataccttgga aaaaatcttg
     9361 gctgaaaatt caggtcagtc aatggaaaaa gtccatgcag atgcagaacg tgataactgg
     9421 atgagcgccc aggaaacact tgaatatggc tttattgatg aaattatggc caacaattca
     9481 ttgaactaat gatgatagaa ggcaaactcg actgggtttg ctttttttgg tataataggg
     9541 agagatttct tagaaagagg atttatcatg tttgaaaaag tcaatcgctc tggcttgatt
     9601 atctatcttt actataatcg tgatgccaaa aaactgcagg attatggaga tattacctat
     9661 cattccaaga aacatcgtta cttacaactc tatgttccaa ctcaagaagt ggagcaattg
     9721 gtcggacgct tgagcaagga aaaatttatt aaaaaagtta gggtttgtca tatccaagag
     9781 ttggaaacac cctttgtggg caatctttat cgataggaaa acgttatcat cgaaaaagtt
     9841 caaggaaaac attgacaatt ttctgataat tcggtatatt cttaacagac tatttaagaa
     9901 ataaggagac aaaaagatga agaaaaaatt tgccctatcg tttgtggcgc ttgcaagtgt
     9961 agcacttctt gcagcctgtg gagaagtgaa gtctggagca gtcaacactg ctggtaactc
    10021 agtagaggaa aagacaatta aaatcgggtt taactttgaa gaatcaggtt ctttagctgc
    10081 atacggaaca gctgaacaaa aaggtgccca attggctgtt gatgaaatca atgccgcagg
    10141 tggtatcgat ggaaaacaaa tcgaagtagt cgataaagat aataagtctg aaacagctga
    10201 ggctgcttca gttacaacta accttgtaac ccaatctaaa gtatcagcag tcgtaggacc
    10261 tgcgacatct ggtgcgactg cagctgcggt agcgaacgct acaaaagcag gtgttccatt
    10321 gatctcacca agtgcgactc aagatggatt gactaaaggt caagattacc tctttattgg
    10381 aactttccaa gatagcttcc aaggaaaaat tatctcaaac tatgtttctg aaaaattaaa
    10441 tgctaagaaa gttgttcttt acactgacaa tgccagtgac tatgctaaag ggattgccaa
    10501 atctttccgc gagtcataca agggtgaaat cgttgcagat gaaactttcg tagcaggtga
    10561 cacagacttt caagcagccc ttacaaaaat gaaagggaaa gactttgatg ctatcgttgt
    10621 tcctggttac tataatgagg ctggtaagat tgtaaaccaa gcgcgtggca tgggaattga
    10681 caaaccaatc gttggtggtg atggattcaa cggtgaggag tttgtacaac aagcaactgc
    10741 tgaaaaagca tcaaacatct actttatctc aggcttctca actactgtag aagtttcagc
    10801 taaagctaaa gccttccttg acgcttaccg tgctaagtac aatgaagagc cttcaacatt
    10861 tgcagccttg gcttatgatt cagttcacct tgtagcaaac gcagcaaaag gtgctaaaaa
    10921 ttcaggtgaa atcaaggata accttgctaa aacaaaagat tttgaaggtg taactggtca
    10981 aacaagcttc gatgcagacc acaacacagt caaaactgct tacatgatga ccatgaacaa
    11041 tggtaaagtt gaagcagcag aagttgtaaa accataatag aaaaatgttg aaatagggaa
    11101 tgagcctttg actcactccc tgtttcgata tttaatactc ttcgaaaatc tcttcaaact
    11161 gcgtcaacgt cgccttggat tatatatgtg actgacttcg tcagtcttat ctacaacctc
    11221 aaagcagtgc tttgagcaac ctgtggctag tttcctagtt tgctctttga ttttcattga
    11281 gtataagaac ctatcaaaaa gtgagggaaa cccctcggaa ttataaatag aaagagtgaa
    11341 tcttatgcta caacaactcg taaatggttt gattctaggt agtgtttacg cgctgttagc
    11401 cctaggatat accatggttt acggaattat caagctcatc aacttcgccc atggtgatat
    11461 ttatatgatg ggagccttta tcggttattt cttgatcaat tctttccaaa tgaatttctt
    11521 tgtagcgctt attgtagcta tgctagcgac agctattctt ggtgtcgtga ttgagtttct
    11581 tgcttaccga cctttgcgcc actctactcg tattgctgtt ttgattacgg ctattggggt
    11641 ttctttccta ttggagtatg gaatggtcta tctggttggt gccaataccc gtgccttccc
    11701 tcaagcgatt caaacagttc gatatgattt gggaccaatt agcttaacaa atgtgcagtt
    11761 aatgattttg gccatttcct tgattttgat gattttgtta caagtcattg tccaaaagac
    11821 taagatgggg aaagccatgc gtgcagtatc agtagatagc gacgcggcgc aattgatggg
    11881 gatcaatgta aaccgtacga ttagctttac cttcgctttg ggttctgctc ttgcgggtgc
    11941 ggctggtgtt ctgattgctc tttattataa ctctcttgag cctttgatgg gggttactcc
    12001 aggtcttaaa tctttcgttg ccgcagtact tggtggtatc ggaattattc ctggtgcggc
    12061 tcttggtggc tttgtgattg gtctattgga aacctttgcg actgcctttg ggatgtcaga
    12121 tttccgtgat gccattgttt atggaatctt gttgttgatc ttgattgtcc gcccagctgg
    12181 tatccttggt aagaatgtga aagagaaggt gtaaacgatg aaggaaaatt taaaagttaa
    12241 tattctatgg ttactccttt tgttagctgg ctatagcttg attagtgtac tggtttcagt
    12301 cggagtactt aatctattct atgtacagat tttacaacaa attggaatta atattatttt
    12361 ggctgttggt ctcaacttaa tcgttggttt ttcaggacaa ttttcacttg gtcatgctgg
    12421 tttcatggcg attggtgcct atgcagcagc tattattggt tctacatcac caacctacgg
    12481 tgccttcttt ggagctatgc ttgtaggggc tttgctttca ggagtagttg ccttacttgt
    12541 cggcattcca accttgcgct tgaaggggga ctatcttgcg gtagcaactc tgggtgtttc
    12601 tgaaattatc cgtatcttta tcatcaatgg tggaagtctt acaaatggtg cggcaggtat
    12661 cttagggatt cctaacttta caacttggca aatggtttac ttctttgtcg tgattacaac
    12721 cattgcaacc ttgaacttct tgcgtagccc aattggccgt tcaaccctct ctgttcgtga
    12781 agatgaaatc gctgctgagt cagttggggt taatacgact aaaattaaaa tcatcgcttt
    12841 tgtctttggt gccattactg caagtattgc tgggtcactt caggcaggat ttatcgggtc
    12901 tgttgtaccg aaagattaca ccttcatcaa ctcaatcaac gttttgatta ttgttgtatt
    12961 tggtggactc ggttccatta caggtgcgat tgtttcggct attgttctgg gaattttgaa
    13021 tatgcttctc caagatgttg ctagtgtgcg tatgattatt tacgctttgg ccttggtatt
    13081 ggtaatgatt ttcagaccag gtggactcct tggaacgtgg gaactgagcc tatcacgttt
    13141 ctttaaaaaa tctaagaagg aggaacaaaa ctaatggcat tacttgaagt aaaacagtta
    13201 accaaacatt ttggtggtct aacagctgtt ggagatgtga ctcttgaatt gaacgaaggg
    13261 gaactggttg gattaatcgg tccaaacgga gctgggaaaa ccaccctttt caaccttttg
    13321 accggtgttt atgaaccaag cgagggaaca gtaaccctag atggtcacct tttgaatggg
    13381 aaatcacctt ataagattgc ctctttggga cttggacgta ctttccaaaa tatccgtctt
    13441 tttaaagatt taacagtttt agacaatgtt ttgattgctt ttggaaacca tcacaaacag
    13501 catgttttta ctagtttctt acgcttacca gctttttaca agagtgaaaa agaattaaag
    13561 gctaaagctt tggaattgtt gaaaatcttt gatttagatg gtgatgcaga gactcttgct
    13621 aaaaatcttt cctacggaca acaacgtcgt ttggaaattg ttcgtgccct tgctacggaa
    13681 cctaaaattc tcttcttaga tgaaccagca gcaggtatga acccacagga aacagccgaa
    13741 ttgactgagt taattcgtcg tatcaaagat gaatttaaga ttacaatcat gttgattgaa
    13801 cacgatatga atctggtcat ggaagtaaca gaacgtatct acgtacttga atatggccgt
    13861 ttaattgctc aaggaactcc agacgaaatt aagaccaata aacgcgttat cgaagcttat
    13921 ctaggaggtg aagcctaatg tctatgttaa aagttgaaaa tctttctgtg cattacggta
    13981 tgatccaagc agtccgtgat gtaagctttg aagttaatga aggagaagtt gtttccctta
    14041 tcggtgccaa cggtgcaggt aagacaacta ttcttcgcac cttgtcaggt ttggttcgac
    14101 caagttcagg aaagattgaa tttttaggtc aagaaatcca aaaaatacca gctcagaaaa
    14161 tcgtggcagg tggtctttca caagttccag aaggacgcca cgtctttcct ggcttgactg
    14221 ttatggaaaa tcttgaaatg ggagctttct taaagaaaaa tcgtgaagaa aatcaagcta
    14281 acttgaagaa ggttttctca cgctttcctc gtcttgaaga acggaagaac caagatgcag
    14341 ctactctttc aggaggggaa caacaaatgc ttgccatggg acgcgctctt atgtcaacac
    14401 caaaacttct tcttttagat gaaccatcaa tgggacttgc cccaatcttc atccaagaga
    14461 tttttgatat cattcaagat attcagaagc aaggaacaac cgtcctcttg attgaacaaa
    14521 atgccaataa agcacttgca atctctgacc gaggatatgt actggaaaca gggaaaatcg
    14581 tcctatcagg aacaggaaaa gaactcgctt catcagaaga agtcagaaaa gcatatctag
    14641 gtggctaaaa caatccagtg gattgtttta gtcggcagat ggagattacg aagtaatcat
    14701 caatatagtc cgggggacct ttttagtcgg tagattgaga ttgcaaacaa atctgcatct
    14761 acattgaaag cttaatttct aataattgaa aaaatcgaat gaaaagtttc ttaccttcat
    14821 tcacagagct cgattttaga gctctttttg ctagcttatt catacttttc taaattttga
    14881 aaaagaaatg taagcgtttg atagatttgc aaaaagattg tataataggg ataagaatag
    14941 aaaaggagaa gtctcatggc agttaaagat tttatgaccc gcaaggtagt ttatattagt
    15001 ccagatacaa cagtatctca tgcagcagat ttgatgagag agcaagaatt gcaccgcttg
    15061 cctgttatcg aaaatgatca attagttggt ttggtgactg agggaaccat tgcacaagca
    15121 agtccatcta aagcaacaag tctttctatc tatgagatga attatcttct gaataagaca
    15181 aaagtaaaag atgtcatgat tcgcgatgtt gtcactgtct caggctatgc tagtctagaa
    15241 gatgcaactt atctgatgtt gaaaaataag attggtattc tccctgtcgt agataaccat
    15301 caagtatacg gagttattac tgaccgtgac gttttccaag cctttcttga aattgcaggt
    15361 tatggcgaag aagggattcg tgtacgcttt gttacagaag atgaagttgg tgttcttgga
    15421 aaaattattt ctttgattgt agaagaaaat ttgaatatat cccatacagt caatattccg
    15481 cgtaaggatg gtaaggtcat tatcgaagtg caaatcgatg gatcaattga tttaccagcc
    15541 ttgaaagaaa aatttgaagc aaatggtatt caagtggaag aaatcgctcg cacttcagca
    15601 aaagtcttgt aagaagggaa gcccaaaggc ttcttttttc atgaaaaggg gattagagca
    15661 aaagatggaa agaaatgata aaatatgcta taatgaaata atgtaaaaaa ggagtattta
    15721 tggacatttc agtaattcgt cagaaaattg acgcaaatcg tgaaaaatta gcttctttca
    15781 gggggtctct ttgacctcga agggctagag gaagagattg ccatcttgga aaacaagatg
    15841 acagaacttg atttttggaa cgataatatt gcggcccaaa aaacgtcgca agaattgaat
    15901 gaattaaaaa acacttacaa taccttccat aagatggaag agttgcagga tgaagtcgaa
    15961 attttattgg attttttggc tgaagacgag tcagtgcatg atgaactggt agcgcagtta
    16021 gccgaacttg ataagataat gaccagctac gagatgactc tactcttgtc agaaccttat
    16081 gaccacaaca atgccatctt ggaaatccat ccaggttctg gtggtactga ggcgcaggac
    16141 tggggtgata tgttgcttcg tatgtatact cgttatggta atgctaaagg ctttaaagtg
    16201 gaagtgttgg attaccaagc aggtgatgag gctggtatta agtcggtaac tttatcattt
    16261 gaagggccta atgcctatgg tctcctcaag tcagaaatgg gtgttcaccg cttagtgcga
    16321 atctcaccat ttgactctgc caaacgtcgc catacctctt tcacatctgt agaagtgatg
    16381 ccagaattgg atgatactat tgaagtggaa atccgtgaag atgatatcaa gatggatacc
    16441 ttccgttcag gtggtgccgg tggacaaaac gtcaataagg tttcaacagg tgtacgttta
    16501 actcacattc caactggaat tgttgtccaa tcaacagtgg atcgtaccca gtatggaaat
    16561 agagatcgtg ccatgaagat gttgcaggct aagctctatc aaatggagca agagaagaag
    16621 gctgcggagg tagattctct caaaggtgag aaaaaggaga tcacttgggg aagccaaatc
    16681 cgttcttatg tcttcacgcc ttatactatg gtaaaagatc accgaactag ctttgaggtt
    16741 gctcaggtag ataaggttat ggatggggat ctagatggtt ttatcgatgc ttatctcaag
    16801 tggcgaatta gctaagatag aaaggaactc acatgtcaat tattgaaatg agagatgtcg
    16861 ttaaaaaata cgacaacgga acaactgctc tacgcggtgt ttcggtcagc gttcaaccgg
    16921 gggaatttgc ttacatcgta ggaccttcag gagcagggaa gtcaactttt attcgttctc
    16981 tgtatcgtga agtaaaaatc gataaaggaa gcctatcagt tgctggtttt aatctggtta
    17041 agatcaaaaa gaaagatgtc ccgcttctac gtcgtagtgt tggggttgtc ttccaggatt
    17101 ataaattgtt accaaagaaa actgtctatg aaaatattgc ttacgctatg gaagtaatcg
    17161 gggaaaatcg ccgtaatatc aaaagacgag tgatggaagt tttggacttg gttggattga
    17221 agcataaggt tcgttctttc ccaaatgaac tctcaggtgg ggagcaacag cggattgcga
    17281 ttgcgcgtgc aattgtaaat aatcccaaag tattgatagc tgatgagcca acaggaaatc
    17341 tggatccgga taattcatgg gaaattatga atctcttgga acggattaac ctacaaggaa
    17401 caactatttt gatggcgact cataatagcc agattgtaaa taccttgcgc caccgtgtca
    17461 ttgccattga aaatggccgt gtcgttcgtg acgaatcaaa aggagagtat ggatacgatg
    17521 attagtagat tttttcgcca tttatttgaa gccttaaaaa gtttgaaacg aaatggttgg
    17581 atgacagtag ctgctgtcag ttcagtcatg attactttga ccttggtggc aatatttgca
    17641 tctgttattt tcaatacagc gaaactagct acagatattg aaaataatgt ccgtgtagta
    17701 gtttatatcc gaaaggatgt ggaagataat agtcagacaa ttgaaaaaga aggtcaaact
    17761 gttacaaata atgactacca caaggtatat gattctttga agaacatgtc tacggttaaa
    17821 agtgttacct tttcaagtaa agaagaacaa tatgaaaaat taaccgagat aatgggagat
    17881 aactggaaaa tctttgaagg agatgccaat cctctctatg atgcctatat tgtagaggca
    17941 aacactccaa atgatgtaaa aactatagcc gaagatgcta aaaaaattga aggtgtctct
    18001 gaggttcaag atggcggtgc caatacagaa aaactcttca agttagcttc atttatccgt
    18061 gtttggggac tagggattgc tgctttgtta atttttatcg cagttttctt gatttcaaat
    18121 accattcgta ttaccattat ttcccgcagt cgcgaaattc aaatcatgcg cttggtcgga
    18181 gctaaaaaca gttatatccg tggaccgttc ttgttagaag gagcctttat cggtttattg
    18241 ggagctatcg caccatctgt tttggtcttt attgtttatc aaattgttta ccaatctgtc
    18301 aacaaatcgt tggtagggca aaatctatcc atgattagtc cagatttatt tagtccgttg
    18361 atgattgccc tactatttgt gattggggtt ttcattggtt cattgggatc aggaatatcc
    18421 atgcgccgat tcttgaagat ttaggtaaaa tagctgcttt tatgaggaga ttgtaaaatc
    18481 tccttttttg ctacaagagt ttttgaaaag agatgcgcag aagaaaagag cttccaaaga
    18541 agtcccccag agaagacttc ttgatggcta atttgaactt gaaatttggc gcactagaaa
    18601 ttttttagaa aaaagtgtaa tttttttata aaagccttta caaaaaaaat taaagtggta
    18661 taattaaatc atagaaagtg caaacgtttt cgtaaaacgt ttgcctaaat aaaaataaag
    18721 gagatttgaa tgatgaaaga tacattcaaa aatgtcttgt ctttcgaatt ttggcaaaaa
    18781 ttcggtaagg ctttgatggt agttatcgcg gttatgccgg ctgctggttt gatgatttca
    18841 atcggtaagt ctatcgtgat gattaaccca atctttgcac cacttgtcat cacaggtgga
    18901 attcttgagc aaatcggttg gggggttatc ggtaaccttc acattttgtt tgccctagcc
    18961 attggaggaa gctgggctaa agaacgtgct ggtggtgctt tcgccgctgg tcttgccttc
    19021 atcttgatta accgtatcac tggtacaatt tttggtgtat caggcgatat gttgaaaaat
    19081 ccagatgcta tggtaactac tttctttggt ggttcaatca aagttgctga ttactttatc
    19141 agtgttcttg aagctccagc cttgaacatg ggggtattcg tagggattat ctcaggtttt
    19201 gtaggggcaa ctgcttacaa caaatactac aacttccgta aacttcctga tgcactttca
    19261 ttcttcaacg ggaaacgttt cgtaccattt gtagttattc ttcgttcggc aatcgctgca
    19321 attctacttg ctgctttctg gccagtagtt caaacaggta tcaataactt cggtatctgg
    19381 attgccaact cacaagaaac tgctccaatt cttgcaccat tcttgtatgg tactttggaa
    19441 cgtttgctct tgccatttgg tcttcaccac atgttgacta tcccaatgaa ctacacagct
    19501 cttggtggta cttatgacat tttaactggt gcagctaaag gtactcaagt attcggtcaa
    19561 gacccactat ggcttgcatg ggtaacagac cttgtaaacc ttaaaggtac tgatgctagt
    19621 caataccaac acttgttaga tacagtacat ccagctcgtt tcaaagttgg acaaatgatc
    19681 ggttcattcg gtatcttgat gggtgtgatt gttgctatct accgtaatgt tgatgctgac
    19741 aagaaacata aatacaaagg tatgatgatt gcaacagctc ttgcaacatt cttgacaggg
    19801 gttactgaac caatcgaata catgttcatg ttcatcgcaa cacctatgta tcttgtttac
    19861 tcacttgttc aaggtgctgc cttcgctatg gctgacgtcg taaacctacg tatgcactca
    19921 ttcggttcaa tcgagttctt gactcgtaca cctattgcaa tcagtgctgg tattggtatg
    19981 gatatcgtta acttcgtttg ggtaactgtt ctctttgctg taatcatgta ctttatcgca
    20041 aacttcatga ttcaaaaatt caactacgca actccagggc gcaacggaaa ctacgaaact
    20101 gctgaaggtt cagaagaaac cagcagtgaa gtgaaagttg cagcaggctc tcaagctgta
    20161 aacattatca accttcttgg tggacgtgta aacatcgttg atgttgacgc atgtatgact
    20221 cgtcttcgtg taactgttaa agatgcagat aaagtaggaa atgcagagca atggaaagca
    20281 gaaggagcta tgggtcttgt catgaaagga caaggggttc aagctatcta cggtccaaaa
    20341 gctgacattt tgaaatctga tatccaagat atccttgatt caggtgaaat cattcctgaa
    20401 actcttccaa gccaaatgac tgaagcacaa caaaacactg ttcacttcaa agatcttact
    20461 gaggaagttt actcagtagc agacggtcaa gttgttgctt tggaacaagt aaaggatcca
    20521 gtatttgctc aaaaaatgat gggtgatgga tttgcagtag aacctgcaaa tggaaacatt
    20581 gtatctccag tttcaggtac tgtatcaagc atcttcccaa caaaacatgc ttttggtatt
    20641 gtgacggaag caggtcttga agtattggtt cacattggtt tggacacagt aagtcttgaa
    20701 ggtaaaccat ttacagttca tgttgctgaa ggacaaaaag ttacagcagg agatctcctt
    20761 gtcacagctg acttggatgc tatccgtgca gcaggacgtg aaacttcaac agtagttgtc
    20821 ttcacaaatg gtgatgcaat taaatcagtt aaattagaaa aaacaggttc tcttgcagct
    20881 aaaacagcag ttgctaaagt agaattgtaa tatacttgag gttggaagct gtattccaac
    20941 ctcttatttt gggagaaaag aatgaaattt ttaacactca atactcacag ttggatggag
    21001 aaagaagcag aggaaaaatt ccagattttg cttgaagata ttcttgaaaa ggactatgat
    21061 ttgatttgtt tccaagaaat caatcaggag atgacctcgt cagaggtgga ggttaatgac
    21121 ctttatcaag ctttgccagc agctgagcct attcaccaag accattatgt tagactcttg
    21181 gttgaaaagt tgttagagca agggaaaaat tactactgga cctgggctta taaccatatc
    21241 ggctatgacc gctaccatga aggtgtggct atcttgtcta aaacacctat tgaagccaga
    21301 gaaattttgg tttcagatgt ggatgatcca acagactatc atactcgccg tgttgcccta
    21361 gctgaaactg tagtcgatgg caaggagcta gcagttgcca gtgttcatct ctcttggtgg
    21421 gataaaggtt tccaagaaga atgggcacga tttgaggctg tcttgaaaaa attgaacaag
    21481 ccacttttac tagctggaga ttttaacaac ccagcaggtc aggaaggtta ccaagctatt
    21541 ttagctagtc cattaggctt acaagacgca tttgaagttg ctcaagagaa aagtggtagc
    21601 tatactgttc cgcctgaaat tgatggctgg aaagggaaca ctgaacccct tcgaatcgat
    21661 tatgtcttta ctaccaaaga gttagcggtg gaaaatttac atgtcgtatt tgatggtaac
    21721 aagagtccac aagtgagtga tcactatggc ttgaatgcta tattaaactg gaaataataa
    21781 ctgaaaagag gttggaacta taaaattcca gccttttctt actagagaag ctactggaaa
    21841 tagcctaaat aagtgagact actgtaatgg aataaaatat ggtataattg ataaggtaga
    21901 tagaatcgag gatgttatgt catttacgaa atttcaattt aaaaactata ttagagaagc
    21961 cttgaaggag ttaaaattta caactccaac agaggtgcaa gacaagttga ttcctattgt
    22021 tttggcaggt cgtgacctag taggagaatc aaaaacaggt tcaggtaaga ctcatacttt
    22081 cttgttaccg attttccagc aattagatga agctagcgat agtgtacaag cagtgattac
    22141 tgcaccgagt cgtgagttgg ctactcaaat ttaccaagta gcgcgtcaga tttcagctca
    22201 ctcagatgtc gaagttcgtg tggttaatta tgtgggtggt acggataagg ctcgccagat
    22261 tgagaaattg gcaagcaatc agcctcatat tgttattgga acaccaggcc gtatctacga
    22321 cttggttaaa tctggtgatt tagctattca taaagccaag acatttgttg ttgatgaagc
    22381 agatatgacc ttggatatgg gattcttgga aactgttgat aagattgctg gcagtcttcc
    22441 aaaagacttg caattcatgg tcttctcagc gactatccca caaaaactgc aaccattctt
    22501 gaaaaaatac ttatcaaatc ctgttatgga gaaaattaag accaaaacgg ttatttctga
    22561 caccattgat aattggttga tttcgaccaa gggacatgat aagaatgctc aaatttacca
    22621 gttgactcag ttgatgcagc cgtatttggc aatgattttt gttaacacta aaacgcgtgc
    22681 tgatgaattg cattcatatc tgactgctca aggcttgaag gttgcaaaaa tccatggcga
    22741 tattgcccct cgtgaacgca agcgaatcat gaatcaggtg caaaatctgg attttgagta
    22801 tattgtcgca acagatttgg cagcgcgtgg gattgacatt gaaggtgtca gccatgtcat
    22861 caatgatgcc attccgcaag acttatcttt ttttgttcat cgtgttggtc gtactggacg
    22921 aaatggccta ccaggtacag ctattaccct ttatcagcca agtgatgact cggatatccg
    22981 tgagttggag aaattgggaa tcaagtttag tcctaagatg gtcaaagacg gggaatttca
    23041 agatacctat gaccgtgatc gtcgtgccaa ccgtgagaaa aaacaagata aacttgatat
    23101 cgaaatgatt ggtttggtta aaaagaaaaa gaaaaaagtc aaaccgggtt ataagaagaa
    23161 aattcaatgg gcggttgatg aaaagcgccg taaaaccaag cgtgctgaaa atcgcgctcg
    23221 cggtcgtgca gagcgtaaag ctaaacgcca aacattttaa tagaaattgt tggagtattg
    23281 agctccaact ttttgcttta tgagaacgaa ctatctaaac cgaaacacta cattaaagac
    23341 tgcaaattgc gattaaaaat ggtataatga taaagttata tagtcccgat aagatggtag
    23401 gtatttatta cgaagagttt tcctatcagt actttgtaac tctataacaa tattttttaa
    23461 ggggggacat ttttatgtca gagcgtaaat tattcacgtc tgaatctgta tctgaggggc
    23521 atccggataa gattgcagac caaatttcag atgcgatttt ggatgctatt ttagcaaagg
    23581 atccagaggc gcacgttgct gctgaaacag ctgtatatac tggttctgtc cacgtttttg
    23641 gtgaaatttc tacaaatgcc tatgtggata ttaaccgtgt ggttcgtgat accattgcag
    23701 agattggtta taccaataca gaatatggat tttctgctga gacggtggga gtacacccat
    23761 ctttggtgga acaatctcct gacatcgctc aaggtgttaa cgaagccttg gaggttcgtg
    23821 gaaatgctga tcaagatcca ctggacttga ttggagcagg tgaccaaggg ctcatgtttg
    23881 gatttgcagt agatgaaaca gaagagctta tgccattgcc aattgcactc agtcataaat
    23941 tggttcgtcg tctggcagaa cttcgtaagt ctggagaaat tagctatctc cgtccagatg
    24001 caaaatcaca agttacagtt gagtacgatg aaaatgaccg tccggtacgt gtagatacag
    24061 tcgttatttc tactcagcat gatccagagg ccactaatga acaaatccat caagatgtga
    24121 ttgacaaggt catcaaagaa gttattccat cttcttatct tgatgataag acaaaattct
    24181 ttatcaatcc gacaggtcgt tttgtaatcg gtggtcctca aggggactca ggtttgactg
    24241 gtcgtaagat tattgtagat acttatggtg gctactctcg tcatggtggt ggtgccttct
    24301 ctggtaaaga tgcgactaag gtggatcgtt cagcctctta tgcggctcgc tatattgcca
    24361 agaatatcgt tgcagcagac cttgctaaga aggcagaagt gcagttggcc tatgctatcg
    24421 gtgttgcgca acctgtttct gttcgtatcg atactttcgg tacaggaaca gtagctgaaa
    24481 gtcaacttga aaaagcggct cgtcaaatct ttgaccttcg ccctgcaggg attatccaaa
    24541 tgctggacct caagcgtcca atttaccgtc aaacatcggc ttacggtcac atgggacgta
    24601 cagatattga tcttccatgg gaacgtttgg ataaggtaga tgctttgaaa gaagcagtaa
    24661 aataagattt taagagggga acgtcctctc ttttttatag tttttaacta tactgggata
    24721 ctgttctgaa aatccatttt gcgaaagtag agatttacgg ct
//
LOCUS       DBEROU010000033        24508 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.033, whole genome shotgun sequence.
ACCESSION   DBEROU010000033 DBEROU010000000
VERSION     DBEROU010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 24508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.033"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(151..291)
                     /locus_tag="ACL8CL_001448"
     CDS             complement(151..291)
                     /locus_tag="ACL8CL_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330679.1"
                     /translation="MKFFWGLLAIIFIKPIIGIVKFFWMIISFAVQLLFYKILDWFFK
                     LI"
     gene            complement(288..470)
                     /locus_tag="ACL8CL_001449"
     CDS             complement(288..470)
                     /locus_tag="ACL8CL_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330680.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGVKVMKTIKKDDNAVVGLGKLFIAD
                     KLMDTARWLIKPEERE"
     gene            complement(472..1251)
                     /locus_tag="ACL8CL_001450"
     CDS             complement(472..1251)
                     /locus_tag="ACL8CL_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330681.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRAGGITIAK
                     QVMKTVPNKALTKMLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
     gene            complement(1530..1880)
                     /gene="rbfA"
                     /locus_tag="ACL8CL_001451"
     CDS             complement(1530..1880)
                     /gene="rbfA"
                     /locus_tag="ACL8CL_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HGS1330682.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            complement(2131..4923)
                     /gene="infB"
                     /locus_tag="ACL8CL_001452"
     CDS             complement(2131..4923)
                     /gene="infB"
                     /locus_tag="ACL8CL_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HGS1330683.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKTEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            complement(4940..5239)
                     /locus_tag="ACL8CL_001453"
     CDS             complement(4940..5239)
                     /locus_tag="ACL8CL_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HGS1330684.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLAKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            complement(5232..5525)
                     /locus_tag="ACL8CL_001454"
     CDS             complement(5232..5525)
                     /locus_tag="ACL8CL_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HGS1330685.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            complement(5547..6683)
                     /gene="nusA"
                     /locus_tag="ACL8CL_001455"
     CDS             complement(5547..6683)
                     /gene="nusA"
                     /locus_tag="ACL8CL_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HGS1330686.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            complement(6727..7206)
                     /gene="rimP"
                     /locus_tag="ACL8CL_001456"
     CDS             complement(6727..7206)
                     /gene="rimP"
                     /locus_tag="ACL8CL_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HGS1330687.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVRIPYSLVSKARLAVKL"
     gene            complement(7332..7967)
                     /gene="trmB"
                     /locus_tag="ACL8CL_001457"
     CDS             complement(7332..7967)
                     /gene="trmB"
                     /locus_tag="ACL8CL_001457"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HGS1330688.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            complement(7964..8758)
                     /gene="ccrZ"
                     /locus_tag="ACL8CL_001458"
     CDS             complement(7964..8758)
                     /gene="ccrZ"
                     /locus_tag="ACL8CL_001458"
                     /EC_number="2.7.1.15"
                     /EC_number="2.7.1.229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0051726 - regulation of cell cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle regulator CcrZ"
                     /protein_id="HGS1330689.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            complement(8919..9530)
                     /locus_tag="ACL8CL_001459"
     CDS             complement(8919..9530)
                     /locus_tag="ACL8CL_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HGS1330690.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMLYNSWPLLYYL"
     gene            complement(9681..9914)
                     /gene="blpZ"
                     /locus_tag="ACL8CL_001460"
     CDS             complement(9681..9914)
                     /gene="blpZ"
                     /locus_tag="ACL8CL_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein BlpZ"
                     /protein_id="HGS1330691.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            complement(9956..10645)
                     /locus_tag="ACL8CL_001461"
     CDS             complement(9956..10645)
                     /locus_tag="ACL8CL_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1330692.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYIALFFLVPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFVFATVGIIFAGLTRYTKSIYPSYAVHVINNIVATFP
                     FLLTFLHRVLG"
     gene            complement(10697..11080)
                     /locus_tag="ACL8CL_001462"
     CDS             complement(10697..11080)
                     /locus_tag="ACL8CL_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HGS1330693.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            complement(11709..12028)
                     /locus_tag="ACL8CL_001463"
                     /pseudo
     CDS             complement(11709..12028)
                     /locus_tag="ACL8CL_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001826248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
     gene            complement(12548..12667)
                     /locus_tag="ACL8CL_001464"
     CDS             complement(12548..12667)
                     /locus_tag="ACL8CL_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGS1330694.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(12771..12920)
                     /gene="blpO"
                     /locus_tag="ACL8CL_001465"
     CDS             complement(12771..12920)
                     /gene="blpO"
                     /locus_tag="ACL8CL_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HGS1330695.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(13164..13412)
                     /gene="blpK"
                     /locus_tag="ACL8CL_001466"
     CDS             complement(13164..13412)
                     /gene="blpK"
                     /locus_tag="ACL8CL_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpK"
                     /protein_id="HGS1330696.1"
                     /translation="MDTKMMSQFSVMDTEMLACVEGGGCNWGDFAKAGVGGAAVVAAL
                     GCAAGGVKYGKILGPWGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(13481..13750)
                     /gene="blpJ"
                     /locus_tag="ACL8CL_001467"
     CDS             complement(13481..13750)
                     /gene="blpJ"
                     /locus_tag="ACL8CL_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpJ"
                     /protein_id="HGS1330697.1"
                     /translation="MNTKMLSQLEVMDTEMLAKVEGGYSSTDCQNALITGVTTGIITG
                     GTGAGLATLGVAGLAGAFVGAHIGAIGGGLTCLGGMVGDKLGLSW"
     gene            complement(14217..14414)
                     /gene="blpI"
                     /locus_tag="ACL8CL_001468"
     CDS             complement(14217..14414)
                     /gene="blpI"
                     /locus_tag="ACL8CL_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpI"
                     /protein_id="HGS1330698.1"
                     /translation="MNTKMMEQFEIMDNEELEIVSGGRGNLGSAIGGCIGAVLLAAAT
                     GPITGGAATLICVGSGIMSSL"
     gene            14695..15282
                     /locus_tag="ACL8CL_001469"
     CDS             14695..15282
                     /locus_tag="ACL8CL_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HGS1330699.1"
                     /translation="MTSYKRTFVPQIDARDCSVAALASIAKFYGSDFSLAHLRELAKT
                     NKEGTTALGIVKAADEMGFETRPVQADKTLFDMSDVPYPFIVHVNKEGKLQHYYVVYQ
                     TKKDYLIIGDPDPSVKITKMSKERFFYEWTGVAIFLAPKPSYQPHKDKKNGLLSKLPS
                     SDFQTKISHCLHCSLKLISHYYQYRWFLLSPRNLR"
     gene            15209..16852
                     /locus_tag="ACL8CL_001470"
     CDS             15209..16852
                     /locus_tag="ACL8CL_001470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0043214 - ABC-type bacteriocin transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HGS1330700.1"
                     /translation="MVLSSLLVTIINIGGSYYLQGILDEYIPNQMKSTLGIISVGLVI
                     TYILQQVMSFSRDYLLTVLSQRLSIDVILSYIRHIFELPMSFFATRRTGEIISRFTDA
                     NSIIDALASTILSLFLDVSILILVGGVLLAQNPNLFLLSLISIPIYMFIIFSFMKPFE
                     KMNHDVMQSNSMVSSAIIEDINGIETIKSLTSEENRYQNIDSEFVDYLEKSFKLSKYS
                     ILQTSLKQGTKLVLNILILWFGAQLVMSSKISIGQLITFNTLLSYFTTPMENIINLQT
                     KLQSAKVANNRLNEVYLVESEFQVQENPVHSHFLMGDIEFDDLSYKYGFGRDTLTDIN
                     LTIKQGDKVSLVGVSGSGKTTLAKMIVNFFEPYKGHISINHQDIKNIDKKVLRRHINY
                     LPQQAYIFNGSILENLTLGGNHMISQEDILRACELAEIRQDIERMPMGYQTQLSDGAG
                     LSGGQKQRIALARALLTKSPVLILDEATSGLDVLTEKKVIDNLMSLTDKTILFVAHRL
                     SIAERTNRVIVLDQGKIIEVGSHQELMQAQGFYHHLFNK"
     gene            16863..18224
                     /locus_tag="ACL8CL_001471"
     CDS             16863..18224
                     /locus_tag="ACL8CL_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HGS1330701.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQRLENNTLTSPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            18281..18409
                     /gene="blpC"
                     /locus_tag="ACL8CL_001472"
     CDS             18281..18409
                     /gene="blpC"
                     /locus_tag="ACL8CL_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HGS1330702.1"
                     /translation="MDKKQNLTSFQELTTTELNQITGGGLWEDLLYNINRYAHYIT"
     gene            complement(18480..19820)
                     /locus_tag="ACL8CL_001473"
     CDS             complement(18480..19820)
                     /locus_tag="ACL8CL_001473"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1330703.1"
                     /translation="MNIAWILLYTLVTNGLEIVIFFKVDGIDLTFERIFKAFLLKILL
                     AFVFVMISYIVGNVYLSYFMEPLYGIGLSFLLLRGLPKKLLFFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDGYSFTGLCIIIFNFFISLAFLKWLDYDFTSLRKEILDKAFQK
                     SLTQINWIMGGYYLVMESLSFFEYEQSIQSKTVRHLILVFYLLFFMGVIKKLDTYLKE
                     KLYERLEQEQALRYRDMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EVYGSVLKDSSQKLQNNKYDLGRLVNIRDKALKSLLAGKFLKARDKNIVFNVEVPEEI
                     QVEGMSLLDFLTIVSILCDNAIEASVEASQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHLNTSLNTTCQNQVFRQVLTVIHTE"
     gene            complement(19834..20571)
                     /locus_tag="ACL8CL_001474"
     CDS             complement(19834..20571)
                     /locus_tag="ACL8CL_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1330704.1"
                     /translation="MRIFVLEDDFSQQTRIETTIEKLLKAHHIIPSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALNYIDKALSAEEFESRIETALLYANSQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSPRAHRVILYTKTDRLEFTASLEEVFKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINKLH"
     gene            complement(20585..20914)
                     /locus_tag="ACL8CL_001475"
     CDS             complement(20585..20914)
                     /locus_tag="ACL8CL_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS1330705.1"
                     /translation="MKHMIIQTQKTVYKVNVDDIYYIQTHPTKAHTVQIVTEEASFNM
                     LQNLSNLENQCGETLMRCHRNCLVNLDKLKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLK"
     gene            21191..21520
                     /locus_tag="ACL8CL_001476"
     CDS             21191..21520
                     /locus_tag="ACL8CL_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HGS1330706.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQTSSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(21688..22737)
                     /locus_tag="ACL8CL_001477"
     CDS             complement(21688..22737)
                     /locus_tag="ACL8CL_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1330707.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWIFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFCQKASKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(22734..23465)
                     /locus_tag="ACL8CL_001478"
     CDS             complement(22734..23465)
                     /locus_tag="ACL8CL_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330708.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTMQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLDQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            23533..23943
                     /locus_tag="ACL8CL_001479"
     CDS             23533..23943
                     /locus_tag="ACL8CL_001479"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS1330709.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            23953..24240
                     /locus_tag="ACL8CL_001480"
     CDS             23953..24240
                     /locus_tag="ACL8CL_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330710.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
BASE COUNT         7726 a         5333 c         4149 g         7300 t
ORIGIN      
        1 aacatatata ctgcaaggtg aagctgacgt ggtttgaaga gattttcgaa gagtataaaa
       61 tgattttact gcttatatta taccatatgg agagagatta aaaaactagc agtggagttc
      121 ctgctagttt tctgcgactt ggattaccat ctagataagt ttaaagaacc aatccaatat
      181 cttgtaaaac agcaattgaa ctgcaaaaga aatgatcatc caaaagaatt tcacaatccc
      241 aataatcggt ttgataaaaa taatggcaag aagtccccag aaaaatttca ttctctctcc
      301 tctggcttaa tgagccaccg agccgtatcc attaacttgt cggcaataaa aagtttcccc
      361 agaccgacaa cggcattatc atctttcttt atcgttttca ttacttttac accttgcacg
      421 gtcaaaaagt aattaccata aattttagct agagttttta tgagtcccat attactctcc
      481 ttcgatgatt tcagcctctt ttcggattgt ttcaacatct tcttgatatt gaacttcact
      541 atagttaact agcttggaca attctttctg caagctttca cccatcggtt tcatagttgc
      601 aaaggttaaa ccacctgaaa tgataccacc caagatagga ataaatttcc ccattccttt
      661 ggccagccct cccttggtaa gattcacacc aaatattctt aagacttttt tcaaaatagg
      721 ataccaaagc atctttgtta aagctttatt aggtactgtt ttcattacct gtttggcaat
      781 tgttatacca ccagcacgta gcaaagcagc agttccattc acccccaaca tgacgcctag
      841 ataaagcaaa agggtatttt gagcatcttc actcaactca tcgcgtgaag cccaaagatc
      901 ctcataacca taaatataac ctaattcttg ggccaatttc agagagaaac cataaaattg
      961 agccacatca gctggaatgg taattgccat agcaagccct ccaggtaatc ctgccaaaac
     1021 agaagtccca ctcgctaata aaacattatc cctaatacag gcattagcca ctcgatctaa
     1081 gatttcttga gataagagag acgttggacc ttgttccaat aaggtaggaa tgtcctgtgg
     1141 acccaattct ttggaaaact tatccactaa aaatttcgaa cgatcaacct taacaactgg
     1201 tagttttacc acttgttgta atacttgcat agctaagtct tgttcagcca tatacaaact
     1261 ccttatttct tttttacacc ttatcatatt atatttccta caaaaaaagc attttctaat
     1321 tagataacat taaaaaatac agaacccctt ttttatagta aaatgaaaca agaacaggac
     1381 aaatcgatca ggacagtcaa atcgatttct aacaatgttt tagaagtaga ggcgtactat
     1441 tctagtttca atatatttta aaagaatatt tttccatttc aaattcaacc tattttcctc
     1501 caccaaaaaa gaggggcaac ccctcttctt tagttcttat ccagattgcg tagcatctcg
     1561 tcaatcttgt ttccatactc gatggactcg tctttgacga aggtcaaatc tgggattttg
     1621 tacaatttca aattgcgacc aagttcacgt ttgatggtac cagttgcttt ttcaagcccg
     1681 atttgggctt tttggttatc cgaagcaagg ttactcaaaa tggtgtaata aaccttggca
     1741 acagacaagt cacccaacat ctgaacatct gtgatggtca caccttggac acgtggatca
     1801 cgaactttct tttgcaaaat ctcattgact tcacgcttga tttccatgcc cacacgatct
     1861 gtacggaaat gatttgccat gatattcctt tctctacttt gaaccaaaag ctaggccagc
     1921 agacctagct attttaaact tcttgatttt cagtttaaat tgaaacgaag ttcaatttga
     1981 agtcatctgc ttctttcgcc gtggtgaaag tgatggaact gatttatcag tcccatcaca
     2041 ggggattttt gagaccctag gctcaaaaat aagcaatgaa accatcggtt tgcttgcgtc
     2101 cctcaccacc taagaaagga gcaaaaaatc ttatctcttg atttcttcca tgacatacgc
     2161 ctcaatcaca tcatccatct taatatcatt gtagccgtcg atcatcaatc caccttcacg
     2221 gccgtttgta acttccttaa cgtcatcttt atagtgtttc aagcttgcga gttcaccatc
     2281 atagataacg acaccatcac ggataacacg gactttagag tcacgggcaa ccttaccgtt
     2341 gataaccata aatccaccga tagttcccac tttagacacc ttgaaggttt cacggataac
     2401 cgcttcacca ataacttttt cttcaaattc tggatcaagc atccctttca tagcttcttc
     2461 catctcttcg ataaccttgt agataatgct gtgaagacgg atttccacat cgtcagcttc
     2521 tgcttgttga cgagcttgtg gtgtagggcg tacgttgaaa ccaacgataa aggcatttga
     2581 agcttcggca agggtcacgt ctgattcgtt gatagcaccg accgctgagt ggacgatagt
     2641 cactttgaca ccttccacgt caatcttttg aagtgaggca gaaagggctt caacagaacc
     2701 ttgtacatca gccttgatga taacattaac agatttgagt tccccagctt taagggtatc
     2761 aaagaggttt tcaaggctaa cacgttgggt agcttgacgt tgtttcatga gggcacgttt
     2821 ggcacgctct tcacctgctg cacgcgcaga tttttcatcc tcgtaaacgg caaagtggtc
     2881 acccgccatc ggtgcttcgt tcaaacctgt gatagagact ggtgttgatg gtccagcaac
     2941 tttaacacga cgaccaaggt cgttggtcat agcacggaca cgaccgaagg tatttccgac
     3001 aacgattggg tcttgaacat tcaaggtacc ttgttgtaca agaagggttg cgaccgcacc
     3061 ttttccttta tccaagcgcg cttcgataac cgtaccgatt gcacgcactg ttgggtctgc
     3121 cttgagttct tggatttcag ctacaagaag gactgtttcc aacaattctt cgatattttg
     3181 gttgaattta gctgagattt caacaaattc agaatctcca ccccaagcgg ttgacatcac
     3241 accatgctct gccaattcac cgataacgcg ttctgggtta gcacctggtt tatcaatctt
     3301 gttaatggct acgatgattg gaacgttagc tgcttttgag tggttgatgg cttcaatagt
     3361 ctgaggcata accccgtcat ctgccgctac gaccaagatc gtaatatcgg taacagaagc
     3421 accacgcgca cgcattgatg taaaggccgc gtgtcctggt gtatcaagga aggtaatctt
     3481 cttaccattt tccacgattt ggtaggcacc gatatgctga gtaataccac ctgcttcacc
     3541 tgtcgcaaca cgtgagttac gaagagtatc caaaagggtt gttttaccgt ggtcaacatg
     3601 tcccatgata gtaacaactg gtggacgctc aaccaattca tcttcattga gataaccatc
     3661 ttcgacaaag aaacgttcga tgtcagcatt atccacttca accttttgtt tggcttcgat
     3721 accgtaatcc accatgagga gttcaattgt ttccccatct aaggattggt tttgtgtggc
     3781 catgacaccc atcatgaaaa gtttcttaac gatttcagct ggttcacgtt tgatacgttt
     3841 tgcgatttcc gcaacggtca taccatctgt atattcaaat tctgttggca attcatggaa
     3901 tttacgctcc gtaacaggtt ttggagtctg attacggttg ttcttgttat tgcctttttt
     3961 gttctttttg ttgttattcc agttactatt cttttgattt ctcacttgat tttgactact
     4021 tcgattcttt tgttgttttc taggaccatc ttcttcatga tcataatcgt cacgattttt
     4081 gtctggtcga gcttgttttt tacgacgtgt atccactgca ggttcttttt tctcagggac
     4141 gacttcaacc actgcttgaa cttgctgtgc ttgttcagct aacttagccg cttcttcaaa
     4201 gatttcctct ggttccttgc gtttgttagc ttgagccaag gcttctttag cagcctgata
     4261 ctgcttgaag cgttcctcac ttgaacgagc gtactctgca ttttgctctg cttttagggc
     4321 tgctgcacgg gctttaaagt caatacgtgg agccgcttga tttgaacgtt tgtcctcttg
     4381 ctgacggcgc tcatttctac gtttttgctg accttgctgc ttcttagctt ggtcattaaa
     4441 gcgacgattg tcgcggttgc tttgaccttg ttttccacca ttacggccat cgtttttctg
     4501 acggtttccg ttttgttgtt ggtcacggtt attgccctta ttttgcttgc gtcgttctgc
     4561 ctgctcttta gcacgggctt cacgctcagc cttgaaatta cggctttgcg gtttcgctgc
     4621 tactttttct gttttcggag caactggctc agccggtttt gcctcttcct tagctacagc
     4681 tggtttagct ggctcagatt tttcggtttt cttttctgca cttacttttg gtgctgcagg
     4741 ttttgcttct actttcggag cagctgcagg cttaaagctg gcagcaattt ttgcagcgac
     4801 agcttcttcc acacttgatg agtggctttt cacatccaag cccaactctt ttgcacgcgc
     4861 tacaacttct ttactttctt ttccaagttc ttttgcgatt tcgtacaatc ttttcttaga
     4921 caaatcatgt cctcctcttc tattccataa gagacctcat tttctttgta aatccagcat
     4981 ctgttacagc caaaaccttt ctcgatttcc cgactgctat gcttaattcc agtgttgaaa
     5041 acacggttac aatttctact tgataataat gacttttatc ttgaatcttc ttggccagat
     5101 tgggtccagc atcatgagct agaaagacca acttggcctt gccgtcttga atggccttga
     5161 ccaccaattc ttcacccgat atgatgcgcc ctgctcgctg agcaagcccc aagagattac
     5221 ttatcttttg cttattcaag tcccaactct cttcttttca ctttgtgatc cacataagcg
     5281 atcaactcgt cataaaagct ttcttccact tccatgctaa agctgcggtt aaagaccttc
     5341 ttctttttcg cctctagggc ttctgcattg tctagtttga tataagcgcc gcggccattg
     5401 gccttgcccg taggatcaat aaagacttgt ccttccttgt tcttgacaat gcggagcaaa
     5461 tcacgcttat caatcacttc gttagacaca acagacttgc gcaaagggat ttttcttgtt
     5521 ttcatctttc cctcctctag cagcttttat tcttctacag tatcgttttc tacttccaac
     5581 tctactgaag cagcgtcttc catggcttca aattcgctag cagacttgat atcgatacgg
     5641 taaccagtca agtgagccgc caagcgcacg ttttgtccac gacgaccaat ggcaagagaa
     5701 agcttgttat ctggaacaac caccaaggca cgtttgctgt cgttttcatc aaagataact
     5761 tggtcaacct cagcaggagc gatggcattg tagataaatt cagctggatc tgctacccac
     5821 tcgataacat cgatattttc ttcgattggt accatgcggt catttttagc atcgtaacga
     5881 gctgggtgga atttgctagt aatcttcttg atattagcac caccacgtcc aacgattgta
     5941 ccgatagcat ccacgtttgg attgtggcta cgaacagcaa ccttcgtacg gtcacctgct
     6001 tcacgagcca cgctcatgat ttcaacagtt ccatcataaa cttctggaat ttcttgctcc
     6061 atcaaacgtt tgatcatttc tggatggcta cggctaacaa agacgttcac accacgaggg
     6121 ttgtcttcaa ccttgtaaac ataaacttcg atacgatcat gagaagcaaa aacttctcca
     6181 ggaatttggt cttgttttga caattgggct tcgatgctac caaggttgac atagataaag
     6241 cggttgtcaa agcgttctac tgtaccagac atgatttctt gctcatgttc tttgtaagta
     6301 ttgtaagtga tggcacgtgt ttgcttgcgc attttttcca tgatggtttg tttggcagat
     6361 tgggctgcta cacgaccaaa ctcagctggt gcttcttcaa acttgatttt gtctccaagt
     6421 tcataagctg aattaatggc aagagcatct ttcaagctga tttccaaacg gctatcaaat
     6481 acttcatcaa caacttcacg gacagtataa actgtaaagt cacctgtttt ttcgttgaag
     6541 tcaatagcta cgctgtctga ctgaccatag cgtctgcgat aagcggaacg aagcgactct
     6601 actactgcgt cgatgatatc ttcttttttg attcccttgt cttcttccaa aatgcggaag
     6661 gcctctagca tttctttact catgttttct tcacttttga atcctcaaaa gctatccttt
     6721 ctttttctat aatttaactg ctaaacgtgc ttttgatact aaactgtatg gaattcggac
     6781 ggttttctta cgcgtcttgt ccatatattc catagtcaac tcgtcctctt cgaaggccaa
     6841 caaagttcct tcaaagacct tttgcttatc gatggcttgg tagagtccga catggatgta
     6901 ttttccaacc gctccagcga cggcatcctt ggttttcaaa ggacgttcca agcctggact
     6961 ggtaatttct aggaaatatt gttctgggaa gggatctggc ttgatggtgt ctaggacagg
     7021 actgataatt tctgtcaagt ctgccgtgtc gttcaaggta attccttcgg gtttatctac
     7081 aaaaatactg agaatcatgt cactgccaat ctttccatac tcgatatcca cgagttcaaa
     7141 aggagcttct atgacaggtt ctacaacttc tctgactaat tctacgattg ttgcgattgc
     7201 gtccacctcc tcataagcaa gaggcgaaga tatttccccg cctcactttt catattctta
     7261 ctatcagtat agcacgtttg ccaacttatg taaagcaaaa gcacttgtac agcctaattt
     7321 taggctatct cttaaaattc tgcctcaact cggtagataa cttgcccctt gtttgagaat
     7381 ttttgctcgt attctgtcat gacattgcct tcaaaatcac tggcatgcaa atctagccag
     7441 acaccattga gtttcatgcc atattgagaa aagctcacta aactgtactc aaacaagcca
     7501 cggttatccg tcttgaaatg aatttctcca ttttcaggca agatacgttt gaaggtgtcc
     7561 aaaaaggtct tgtaggtcaa acgacgcttt tcatggcgtt ttttcggcca tggatctgaa
     7621 aagttcagat acaagcgatc aatctcaccg tcttcaaagt agtcagttaa gtcagaacca
     7681 tctacccaca agagcttgat gttaggcact ccaacttcaa gcaccttgtc caaagcgtag
     7741 ctcaaaacag acttttgaat atcaatcccg atatagttga tgtcagggtt ttgcttggcc
     7801 atacctgaaa caaaggcacc ctttccactt ccaacttcca catgaatggg attatcattg
     7861 ccaaacaagt cccgccattt tgccttggct tccaagggat tgaggaccac atactgggga
     7921 tttgcctcta gtaattctgt tgccccttta cgatttctaa ctctcatctt ctctttccat
     7981 acttgtctcg gaaatgacgc aaaccatgaa tctcccgatt gacattttct aaatcttggt
     8041 tcatataata cttggaaatc tgactcaaat aagacaattg accataccaa tacaatttac
     8101 ttaataccgt ttgattgtac ttgtaaccgt agtaggtcaa ccattccttc cactgatgtt
     8161 ctgaaatata atggcagagc atatgggcca catcaaacat gcgatcggtc aagcgaaccg
     8221 aatcccaatc tactaaataa atcaagccac tatctgtctc aatccaatta ctatgtcgta
     8281 catctccatg gacaatggtc gcatggtcct ctctaaatcc tggaatagtc tgacgtaaat
     8341 cagccatcac ttcactgata aaatgatttt tacgcaaagc atctggagcc gtttcctgcc
     8401 aagactgtag taaatctaca ggtgtttcca tggcatagcc caaacgactc aattgtgtca
     8461 tcaacggacg tgagcgatga agacgggtta aaatattgac gatttgctta cgattcatat
     8521 catagggggt caatatcttg cctgtcaacc attcttgagc acacatatca cgcccatctg
     8581 ccaaacgacg actccataat aattgtggag caatttgttc tctagctaga ccaggtagga
     8641 ttggagaggt gttcattttt acaaagatgc gcttcccatc aggatagcta cccatataag
     8701 ccttgccact tttcccaggt atgggagtca gtgttagctc attatcaccc aaatccattt
     8761 ctttcctccg tataaagttt ccttactatt ctactagttt ttggcctatt cgtcaaccac
     8821 tctaacccga ttctcaaaga aaagcctctg gattggcttg ggtatcatta tagcttgaaa
     8881 aaggtaagca ccgtcttctg cttacctttt cataattttt ataaataata taagagtggc
     8941 caactgttgt aaagcatatg gactagagta gaaacccata ggttttgact ctttttataa
     9001 gcaaagccca gcaacaaacc cccaagtaga taaaaaataa acgaagggac attagaaggt
     9061 ccatgcatga aagaaaagag agaggaagtt agcacaagcc ctaaccaaga attgtcaaaa
     9121 accgcacctt gaagaagtcc tctgaaaatg agttcctcct ggatggggcc aaaaactgag
     9181 gtagttaaaa tcaaagacag ggaaattcct ctgctgactt ccgccaagtg ttggacccct
     9241 gagccagaag aaacagcgaa gaaatgaata taaaccagcg tctcaagtac acttagaaga
     9301 aagattgcaa tgaaaagcaa aagttgtttc ttgcttaaca tcccaaaaga aatcatttca
     9361 aactttctag cataagcaac actcagcgag gttacaagaa gacttacaag aatcaatgca
     9421 gattcatttt gaaaaaaacc cccatggtta atcgtataag gaactgtaat gacaattatt
     9481 agtactaaaa atccaaaaca caaggcatga aatttatcaa aaaactccat aaaattatcc
     9541 tcctcaacaa acagacttcc tacacgtcat tctttagttc tagcctttcc aaaacaaacc
     9601 attttatact cttcgaaaat caaattcaaa ccacgtcaac gtcgccttgc cgtactcaag
     9661 tacagcctgc ggctagtttc ctagtttgct ctttgatttt cattgagtat tagtaaccaa
     9721 aatccgacca catagccagc ccctatgaat atagccatta aagctagcat ggaatttagg
     9781 aaattaaaaa ccaccgcaga tacaaaggtt agcacaaaaa cattaaaagc aatggtgtca
     9841 gaagccaaga ctagaatata gggtgtcaac cgatctaaag ttttggaatc taggaaaaat
     9901 aagtgtttat acatgatgac ctcctctatg gctgaaaagc aagccttttg tttttttacc
     9961 ccaagaccct atgtagaaaa gtgagcaaaa acgggaaggt cgctacaata ttattgatca
    10021 catgcaccgc ataggatgga taaatgctct tggtatagcg ggtcaaacca gcaaagatga
    10081 ttccaactgt tgcaaagacg aagatatcta acagactagg caggcttgaa aaatgaggga
    10141 gagcaaataa aatagaagga agaagcaaat caagaccaaa tcgcgaatgc ttaaagaaag
    10201 catgttgcag taatcctcta taaatcaatt cttccatcag tggaaccaga aagaacaggg
    10261 ctatataaat acctagctct gcaaagttag tcccactata accgatcaat acagcccaac
    10321 cttcagcagt tgactgaaca tgtttagctg tctgaacgtt aaaagagatc tggagcacta
    10381 ccactaatac tgtcaaaatc gaataccaaa gccatttttt tcttggaatg tgaaagagat
    10441 aaccatgacc tgtcttaacc agaatccaaa tcatgactcc gctaaatagc aaactcaaga
    10501 aattttgaat ccagacgaaa ttgccaatct gggaagaaaa ttgccaataa ttttgaataa
    10561 taagcgtcat ctgagaaaga ccaaatacga aaaataagta agagaaaact gcacttattt
    10621 tgaatagaag ttgatacttt ttcatagaaa tcctccctac tatgacctca ccttgtcagg
    10681 ctctactgct gtaagattaa gaagacagtt tgtttttttt aaggctaacc tgactactag
    10741 ataatagata cattaaggca ttaaagacaa tgaaaatatg tccatagaat aaaatcaacc
    10801 tcgcatccaa accaagataa agtttgatta tcaaaaagat gagcaaaaga atttgaaacc
    10861 ataaggtttt tccaaaaata aatttaaagc gatttcgaat atctacttcc ttgattttta
    10921 ccgccacccc tttattagca agaaggaaaa ctcctgcttc aaacaaacca ctgtaaagaa
    10981 caagccaccc aatagatacg atagagattt gtaaaaatgt ccctaaaaga atatccaaca
    11041 cactactcaa gaaaataaca aaaaataatc tgtatttcat attaaatacc tccattcatt
    11101 tatttcacta acaatttaat agagccttct actcaaatat cctgtcagaa aaggatagaa
    11161 agctactttt tataatactt caagccccac atgagcagaa gcgtgataaa caagcagaga
    11221 atacacctat ataagcgatt agttgttgat agaattctgt ttctgaaata cctctataca
    11281 aacaaatgac aaacataaaa tctgccaagc cgataaacat aagttgattg gttctaggac
    11341 taaccaaatc atcatttact tatatttaag agtatctctt ttatgttaat gtatgttagc
    11401 actgaaaagc aagacaggcc aataatattt aaaatgaaca gtaacggggt taagtctcta
    11461 aaaaaattat ctactgacac tacaagaaat actatacata ttatagtcga aactatcttt
    11521 ttcttatcca taattattta ctcctttcct aacaaatcca acttatcaat caagagcgat
    11581 ttttaacata atgtagcagc acccgttgca actttgacaa gtttagtata tcattgtttt
    11641 ttaaaatttt tcatccaaat cttgaattgt catcgaaacc tcctaaattg caaaaacttc
    11701 attagcccct actttttata atacttcaaa ccccaaatga gtagaagcat gatgatcaag
    11761 caaagaatag caccaatata agcgattagt tgttgataag attctcctgc tgtgatacct
    11821 ctatacaaac aaataataga cataaagcct gtcaagccga taaacatgag ttgattggtt
    11881 ctaggactaa ccaaatcatc atcttcaaac tctcttaatc tcatttccct agtaagataa
    11941 acagtaacca aaatagaagt caagttaata actactaaaa gaaattggaa aactgacgaa
    12001 atagaaatag ataagtagaa acaagcaaag gcaactgacc caggaacaat ctcacaaaga
    12061 atatgattct ttttaatcaa gaaaatcttt catttctttt ctttcttttt aattaaagaa
    12121 aaatagatca taaccgcaat catataggct atggtataaa atagattata ccaaatactc
    12181 tccctaagcg gataaagaaa gatggacatg atcagataca gaacgaaaat aatcagtatt
    12241 tttttcttca taagttttcc tcctaaatgt gcgatttatc ttagttgagc aagaacattt
    12301 acactgctag tatagcactt attttgacct tggatcactc aaatcataaa tggtcatcaa
    12361 aacctcttga attgtaaaaa ttaaaaaagc aagcatgaaa aacatacttt cctctttata
    12421 ttgtattgat accaacttgt ttgtagactt ttcatcctgc tatcacatat cattttgaca
    12481 ggcgaaacaa tattaaagaa actcccctgt aaattaagct agcaaataca ggggagaaat
    12541 ttatttttta gagagtacta tccgtatcct ttttggaaga ttttgaaaat atttttctaa
    12601 ttaagtcatc catataagga ccaaatatac caactactaa accaataata aaacttttaa
    12661 aatccataat taccaccaac atgttgctgc ataggctaca cctccaagta tagctccacc
    12721 tgcagcacca gttacaccta ttcctatagc aaatggtccc aatagaaatg tcaaaccgtt
    12781 gttgcacacc catcaattgc gccatatgca acccctgctg cacaactaat ttttcttccc
    12841 caatcaatat ctccaccttc aacgcaagca agcatttcat tatccataac agaaaattgt
    12901 gacatcattt ttgtattcat atagtatgtc acttttcagt tacggaacaa gtttaatata
    12961 aaaattatca aaaaaacata ggcaataaag agaaaaatta atttatcata gattagaaat
    13021 aatatgacaa aacaattcaa tgatgttaat tcaatagtct tttgtttttt atcagagata
    13081 cttatggata gataaataag ataggtttga aaagcgaaga gaataataaa gaatatagcc
    13141 ttcataaaat ttagctttca tttttatgat gtagcggtat aggctaaata tccacaaacc
    13201 actgctcctc caattcctcc tattgcagcg ccccatggtc ctagaatttt cccatatttc
    13261 actccacccg ctgcacaacc taaagcagca actacagctg ctcctccaac acctgctttg
    13321 gcaaaatctc cccaattgca tccgccacct tcaacgcaag caagcatttc agtatccata
    13381 acagaaaatt gtgacatcat ttttgtatcc atgacaaata ctcctttttt aaaaaactaa
    13441 aataaatcag aatagaatcc tcataatttt actataagtc ttaccaactt agtcccaatt
    13501 tatcaccaac catacctcct aagcatgtta atccaccccc aattgcacca atgtgtgctc
    13561 caacaaatgc accagcaagt ccagctactc ctaaagtggc caaacctgct ccagttccac
    13621 cagttataat tcccgtagtg actcctgtaa tcagtgcatt ttgacaatca gtggagctat
    13681 accccccttc aactttcgca agcatttcag tatccataac ctctaactgt gacaacattt
    13741 ttgtattcat gatgaatacc tcctttttat tttcaatttg ttaccaaagt cttaaattca
    13801 ataaacaaat agatttttta tagtatcttt ttgattttct taaaaaagta tatacgtcta
    13861 ctatcttctt aaaggtagca gtacctattt tttagtctaa gatttcaata atcttgagta
    13921 tctaaaatat cttaatttcg ttattctcct tgcaataaaa agttttacta tactatttat
    13981 taacttgcag aaagcaaaaa atattagtaa ataatagttt atagttaagt tttttattcc
    14041 taccaatcca tcaactaagt aaagcatcaa cgattacata aacgattgat aatataatta
    14101 aaattttgct aactatctta ttctcatcat tcttagataa ctttgatatt ttgtaagtaa
    14161 gtaaataaga cagtaaatta atagcgataa taatactata tttaagaatc ataatcttac
    14221 aaagaggaca taattcctga acctacacaa ataagtgttg ctgctccccc agttatcgga
    14281 ccagtcgcag cagctaatag tactgctcca atacaaccac cgattgcaga tcctaaattg
    14341 cctcttcctc cactaactat ttcgagttct tcattatcca taatctcaaa ttgttccatc
    14401 atttttgtat tcatgacaaa tactcctttt tcttttttta tttttgtctt gttgtaactt
    14461 tgataagttt agtatatcat cgttttttaa aatttttcat ccagatcttg aattgtcatc
    14521 gaaacgtctt gaattagctt ttttatttca agccacctct aaatgtttaa aaaaaataat
    14581 ttctaatcac ttttttacca ttcaggaagt tttaatgact attcaagatt tcataaaata
    14641 tgaacttagt tttatgacat aatagaccta tccactatat gaaaagaatt gccaatgact
    14701 tcttataaac gtacatttgt tcctcaaata gatgcgagag actgtagtgt cgctgcctta
    14761 gcctcgattg ctaaattcta tggttcagat ttttctctag ctcacttgag agaacttgca
    14821 aagaccaata aagaagggac gactgctctt ggcattgtaa aagccgctga tgaaatgggc
    14881 tttgaaacaa gacctgttca agcagataaa acgctctttg acatgagtga tgtcccctat
    14941 ccatttatcg ttcacgttaa caaagaagga aaactccaac attactatgt tgtctatcaa
    15001 acaaagaaag actatctgat tattggtgat cctgaccctt ctgtaaaaat cactaaaatg
    15061 tcaaaagaac gctttttcta tgaatggact ggagtagcta tttttctagc tcccaaaccc
    15121 agctatcaac cccataaaga taaaaagaat ggtctactaa gcaagcttcc ttcctctgat
    15181 tttcaaacaa aaatctctca ttgcttacat tgttctctca agcttattag tcactattat
    15241 caatataggt ggttcttact atctccaagg aatcttagat gaatacatcc caaatcagat
    15301 gaaatcaact ttaggaatca tctcagttgg tctggttatc acctatatcc tccaacaagt
    15361 catgagcttc tccagagatt atctcctaac cgttctgagc cagagattaa gcattgatgt
    15421 gattttatcc tatattcgcc atatttttga acttcccatg tctttctttg cgacacgtcg
    15481 tacaggagaa atcatttcac ggttcacaga tgctaactct attatagatg ccttggcttc
    15541 taccattctt tctcttttcc tggatgtttc tattctgatt cttgtagggg gcgtcttact
    15601 ggcacaaaac cctaatctct tccttctttc tcttatttct attcctatat acatgttcat
    15661 catcttttct tttatgaagc ctttcgaaaa aatgaaccat gatgtcatgc aaagtaattc
    15721 tatggttagc tctgccatta tcgaagatat caacgggatt gaaactataa agtcgctcac
    15781 aagtgaagaa aatcgctatc aaaatataga cagcgaattt gtagattatt tggaaaaatc
    15841 ctttaagctc agtaaatatt ctattttaca aacaagttta aagcagggaa caaaattagt
    15901 tctgaatatc cttatcctat ggtttggcgc tcaattagtc atgtcgagta aaatttctat
    15961 cggtcagctg attaccttta acacacttct ttcttacttt acaactccta tggaaaatat
    16021 tatcaacctc caaaccaaac tccaatctgc gaaggttgct aataaccgtt tgaacgaagt
    16081 ctatctagtc gaatctgaat ttcaagttca agaaaaccct gttcattcac attttttgat
    16141 gggcgatatt gaatttgatg acctttctta taagtatggt tttggacgag ataccttaac
    16201 agatattaat ctcacgatta aacaaggaga taaggttagc ctagttggag ttagtggttc
    16261 tggtaaaaca actttagcca aaatgattgt caatttcttt gaaccctaca aaggacatat
    16321 ttccatcaat catcaggata ttaaaaacat tgataaaaaa gtcttgcgcc gtcatattaa
    16381 ttacctacca caacaagcct atatctttaa tggctctatc ttggaaaact taactttggg
    16441 cggtaatcat atgattagcc aagaagatat tctaagagct tgtgaattag ctgaaattcg
    16501 tcaagacatt gaaagaatgc ctatgggcta tcaaactcag ctctctgatg gagctggtct
    16561 atcaggagga caaaagcaac gaatagccct cgctcgtgct cttttaacta aatctcctgt
    16621 tttaatacta gacgaagcta ctagcggtct tgatgtcttg actgagaaaa aggttataga
    16681 taatcttatg tctctaactg ataaaaccat tctctttgta gcccatcgtc tcagtatagc
    16741 cgaacgaacc aaccgtgtca ttgttcttga ccaggggaaa atcattgaag ttggtagtca
    16801 ccaagagtta atgcaggcgc aaggtttcta ccatcatcta ttcaataaat aaggagaatg
    16861 tcatgaatcc taatcttttt agaagcgtcg agttttatca gagacgttac cataactatg
    16921 cgacagtgtt aattatacct ctttcattac tatttacttt catcttgatt ttctcccttg
    16981 ttgccacaaa agaaattact gttacttccc aaggagaaat cgcccctaca agtgtcattg
    17041 cctccattca gtcaaccagt gataatccta tcctagctaa tcatttagtg gcaaatcaag
    17101 tagttgaaaa aggggactta ctcatcaaat actctgaaac aatggaagaa agtcagaaaa
    17161 ctgccttagc aactcaatta caaagacttg agaagcaaaa agaaggactt ggaattttga
    17221 aacaaagctt agaaaaagcg actgatcttt tttctggcga ggatgaattt ggctaccata
    17281 atacctttat gaattttact aaacaatccc atgatattga actgggtatc acaaagacta
    17341 acaccgaagt ttcaaatcaa gctaatcttt ccaatagcag ttcatcagct attgaacaag
    17401 aaattacaaa agttcaacaa caaattggag aatatcaaga gttgagagat gctatcataa
    17461 ataacagagc acgcttacca actggcaatc cgcaccagtc aattttgaat cgttatcttg
    17521 tagcctcaca aggacaaaca caaggaacgg cagaggagcc atttttatct caaattaatc
    17581 aaagtattgc aggtctggaa tcatctatcg caagcctcaa aattcagcaa gctggtatcg
    17641 gaagtgtagc aacttatgat aacagtttag caaccaaaat tgaagtactc cgcactcagt
    17701 ttttacagac agcctcacag caacaactaa ctgtggagaa tcaattaaca gaattaaaag
    17761 tacaactaga tcaagccaca cagcgtttgg aaaacaatac cttaacctcc ccaagtaaag
    17821 gtatcgttca tctgaacagc gaatttgaag gtaaaaatag aattccaact ggtacagaaa
    17881 ttgctcaaat attccctgtc atcacagata caagagaagt actaatcact tactacgtat
    17941 cttctgacta tctacctcta ctagataaag gacaaactgt aagattaaaa ctggagaaga
    18001 ttggaaatca cggcaccacc atcatcggcc aacttcagac aattgatcaa actcctacca
    18061 gaacagagca aggaaatctc tttaaattaa ccgctcttgc aaaactatct aacgaggata
    18121 gtaaactcat ccaatatggc ttacaaggtc gcgtcactag tgtaactaca aagaaaacat
    18181 attttgatta tttcaaagat aaaattttaa cacattctga ttaattttca gataacactc
    18241 tataactatt tattatctta tcaaaaagga gaatcataac atggataaga aacaaaacct
    18301 aacttcattt caagaactaa caactaccga actcaaccaa attacaggtg gaggattgtg
    18361 ggaagattta ttatataaca ttaatagata tgctcattac atcacataag aacttcatca
    18421 tccaatacaa ctataaaaaa ataagaccga gaaacaagta ctctcggtct tatttttcat
    18481 cattctgtat gtatcacagt aagtacctga cgaaagactt gattttgaca ggtggtattt
    18541 agactggtat taagatggct ttccacaatc ttcatgacgg tatagagacc aactcctctc
    18601 tcctcccctt tagaactggc tccaaaggag aagatttcag aaatatcgat gccctcttct
    18661 ttgatggagt tttcgatgat aaaggtctcc tgtgctccat tttttaaaaa ggcgattgaa
    18721 acatgaggtt gactagcttc cacactggct tcaatagcat tgtcacaaag gatagacaca
    18781 atggttagaa aatcaagtag actcatcccc tcgacctgaa tctcctcagg aacttcgaca
    18841 ttaaagacaa tgttcttatc tctggctttt aaaaatttcc ctgctagaag acttttgagg
    18901 gctttatcac gaatatttac caatctgccc aggtcatatt tattgttctg caatttctga
    18961 ctggaatcct ttaagacgga gccatagacc tcttttatct gctccatatc ctcctcttca
    19021 atgcccagac gtaagctagt caagaggttg gtataatcat gacgaaagct ccgtacttcc
    19081 ttgtaaagct cctctatatg ccgactatag cgttccatat ctctatagcg cagggcctgc
    19141 tcttgttcca atctctcata gagtttttcc ttcaaatagg tatccaattt cttgataacc
    19201 cccataaaaa agagtaggta aaagactagg atgagatggc gaacagtctt tgattgaata
    19261 ctttgttcat attcaaaaaa agacagactt tccatgacta aatagtagcc acccattatc
    19321 cagttaatct gagtcaggga cttttgaaag gctttatcga gaatctcctt tctcaagcta
    19381 gtaaaatcgt agtccaacca tttcaaaaaa gctagagaaa tgaagaaatt gaaaattatt
    19441 atacataacc cagtaaatga gtagccatca tatacttgcc cttgtcccaa aaatggaagc
    19501 acaaaatagg agactcctct ataaaagaga ttcaccaata tcattggaaa gagaccataa
    19561 aagaaaagga gttttttagg aagccctctc aataataaga aagataagcc tatgccgtac
    19621 aagggttcca taaaataaga taggtaaaca tttcctacta tatagctaat catcacaaaa
    19681 acaaaggcca acagtatctt caaaagaaag gccttaaaaa tcctctcgaa agtaagatca
    19741 attccatcca ccttaaagaa gatgacaatt tctagtccat tagtaacaag tgtatacaac
    19801 aatatccaag caatgttcat aaattctcct agctcagtgt aatttattga tggcctcaga
    19861 cacttccctg accttataac gggcgattag acaacttcca ccattgggaa agaaaagcag
    19921 tttttctttc ttatccaaat gcaccacatt tgcaggattg atgagaaaag agcggtggca
    19981 ctgcaagaga cggggctcct gcttgaaaac ctcctctaaa ctcgctgtaa attccagcct
    20041 gtctgtcttg gtatagagaa taacacgatg ggctctgggc gacgtttcga gatagtaaac
    20101 ctctttaaaa ggatactgaa attgggcaaa ttttgattta aagtaaaagc aatcttccgc
    20161 cagactttta ctatcttgac tattggcata gaggagggct gtctcgatcc gagattcaaa
    20221 ctcctctgct gacaaggcct tatcaatgta gttcaaagca gacacttggt agcgaaaaga
    20281 caggggcata aactccgagt gagtcgtcac aaagacaatc agggcataag gatcccgatc
    20341 ccgaatcttt ctagccactt ccagtccctt catctcttca tttcgaatct caatatccaa
    20401 aaagaatagc tgatgggccc ccttctcatg cacttcagcc agcagttggt ccggcttgcc
    20461 aaatacctca aaagagctag gaatgatatg atgtgctttc aaaagtttct caatcgtcgt
    20521 ttcaattcta gtctgttggg aaaaatcatc ctctaaaaca aatattctca tctttttact
    20581 ctccttattt taaccatttt tgacgaattt ctctatagcg acgtctggca tacttgacag
    20641 cgtattgacc ttcttctccg agaaaaagga ttctttcttg aaaatcaatc gattttaatt
    20701 tatcaagatt aaccaaacaa tttcgatgac atctcatcaa ggtttcccca cattggttct
    20761 caagattact taaattttga agcatattaa aactagcttc ttctgtaaca atctgtacgg
    20821 tatgggcttt agttggatgt gtttggatat agtagatatc gtcgacgttt actttataga
    20881 ctgttttctg cgtctgaata atcatatgtt tcattggatt ctcctcctaa gaaaagaata
    20941 acagaaaagg tctagcccaa gcccacccaa tcctaaacag tctttgaaag ctcctaaaag
    21001 gattattttt ctacaacatc atgatgagct tacaactatt ttatcataaa attcactcag
    21061 cgcaattcaa gacatttcaa tgacaattaa agatttgaat aaaaaaaaag aattaaaaat
    21121 gataaactaa cagtatcaaa acacagttgc cagaagtaag actggcaccc agattaaagg
    21181 aggcattccc atgacagata cagaccccat caaaagagct catactttga ttactgactt
    21241 aaacaaagcc taccaagcat gcaaacaggc aagcgctgac gacgtccgct ttcaggagca
    21301 attaaactct attcttggtt ttctagccaa ggctgaaaca gtggataatc gattcttgat
    21361 tgaactagaa aaattttacc agacttccag tcttctcatg ggactcagcg ctcttgatcc
    21421 agatgctcca actcgcgccg cttggcgggc ctatgaccgc ttccactttg accaagtcaa
    21481 gaccaagtta atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaagctgta
    21541 cttgagtacg gtaaggcgac gctgacgtgg tttgaagaga ttttcgaaga gtataagtct
    21601 ctacggacca accattatcc tgtagtaaca aaaaagaagc tgagacagac tgaactcaac
    21661 ttctttttta gtgtcgtatc agcaatgtta gtcctgcatc tgacgtttta cctgataagg
    21721 caaatacaag actagtaaaa ccaaaccagc tcctagtaag gctagaaggg ccagtttttc
    21781 ttggaaggta atcaacccaa caactaattc cacaagtaaa acaaaactgg tcaatcctcg
    21841 aactacctcc tgtaagcctt tttccttttg ccccttgtcc agcggaaaga gttgggtcaa
    21901 atactggtag tcaaaggcat gatagagggc cagcaactgg aagagcaaga ggtagttaaa
    21961 gagaactacc actgctgtcg caatccaagc ttgctcgata aaaacctgcg ccagtacgga
    22021 aagtaagaga agacgaagac tgagagcaaa gaggtcgcca tttcgcagat aagaacgcag
    22081 atagagattt tgccaaatct tcccaggcac cttctgaaca gcctttaaaa taaagtccag
    22141 ataggcacga cgcttaacgc tgtttgaaat tcccttgacc tgcgtaaaga gggcaaagaa
    22201 acgaagcaag acttgcttac gcttgctttc ttgagaaata agatagtccc agtccagtcc
    22261 agtttcagtg aaaaatttgc tggccttttg acaaaagtgg aaatattttc ctacccccaa
    22321 taaaagcaca tagagcagaa aaactggcaa gccataaccc attgctaaaa ataaaggcgc
    22381 aaataaaagc aagaagaggg tctggacaaa aatccaaaag actagggaaa tgccagtttg
    22441 acgcttgaga tggagcttaa tttcctcttc tccaactaag agaaagagct tgtctggagc
    22501 ctccatatag gtggcaattc ctccccaaag taaaagtaaa acagacgtaa ttcctacaaa
    22561 caaaaggata ggccaatgat tttcaggaaa atgttgtaag agttgactgt actggtaggc
    22621 tagaaagccc aacaggacaa gcaggaacaa gacaaagtgg tcattgagca catagcgcag
    22681 ataaccaaga cactccttac gaaaggcctg ctttctcttt aaaaacaagt ctttcatagg
    22741 tcctcctctt ttgtcagagc caagtaaata tcattcaaac tagcctcagg catatcaaag
    22801 gcttcgcgta gttgcaggag attgcctttg gcacgcacct ctcccttgtg aagaatgaca
    22861 aaggcatcac acatcttctc cgccgaatcc agcacgtggg tactcatgag aatagacttg
    22921 cccttttgct tctccacttc caaaagctga atcaaatcag aaatagctag cggatcaaga
    22981 ccaaggaaag gctcatccac gatgaaaaga cttggatcca ccacaaaagc acagataatc
    23041 atgaccttct gcttcatccc ttttgaaaaa tgaacaggga accagtctaa tttctggtcc
    23101 agacggaaca tctttaacaa gggctctact cgttcgaaag ccactttttg ctcaataccg
    23161 taagccatag caaccgtttc gatatgctct ctgagggtca attcctcata cagactaggc
    23221 gtctcaggaa tgtagccaat ctgcttgcgg tagctagtcg catctccttg catagtcagg
    23281 ccattgatat tgatggagcc actataaggt gccaacagac cgataatctc attgatcgtc
    23341 gttgatttcc cagcaccatt gagaccaatc aaaccgacca actgcccact ttcaacagta
    23401 aaggacacat ctttcaaaac aggaacatga acatagccac ctgtcaggtt tttaatttct
    23461 aacatatttt ctccaaatct ggtataatgt agctatatta tatcaaaatt cagtacagta
    23521 gaggtagatt ttatgtcaga ttgcattttt tgtaaaatca tcgcagggga aattcctgct
    23581 tcaaaagtat atgaagatga gcaggtcctt gcctttcttg atatctctca agtaacacta
    23641 ggacacacct tggtcgtgcc aaaagaacac tatcgcaatc ttttggagat ggatgctacg
    23701 agcgccagcc aactctttgc ccaagtacca aaagtagctc aaaaagtcat gaaagtcact
    23761 aaggctgctg gtatgaatat catttccaac tgtgaagaag tcgctggtca aacagttttt
    23821 catactcacg ttcaccttgt gcctcgctac agtgctgacg atgacctcaa gattgatttt
    23881 atcgcccacg aaccagactt tgacaaactt gctcaagtcg ctgaaaccat caaaaacgct
    23941 taaggagttg ctatgaaatt atccaaccta ctgctatttg caggagctgc agccggaagt
    24001 tatctggtta caaaaaatcg ccaaaccatc acagatgaag tcttgaatac cactgaccgc
    24061 gttcaagcta tcaaggacga tgtggatatt atccaaaaca gcctgcaaat cattaaccag
    24121 caaaaagaac ttatcaagga ataccaagaa gacttgactt acaagtttaa ggtcttggaa
    24181 aaggatatcc agactagact agctgtgata aaagaaatgc agggaactga agataagtaa
    24241 aaagagcccc ccggctcttt tttaaaacgt aaacaagtta tagtgaattg aatttagaat
    24301 agcacaccat agtttctaaa gcattgttag agattacttt aaaatcctct tatcaatttg
    24361 ttcatattct attcaatttg ttcatattct atttcaatct attatatata tcccatcaac
    24421 tatgcatcat aatttaggta actcatactc aataaaaatc aaaaagcaaa ctagaaagct
    24481 aggcacagac tgctcaaaac accgtttt
//
LOCUS       DBEROU010000034        23794 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.034, whole genome shotgun sequence.
ACCESSION   DBEROU010000034 DBEROU010000000
VERSION     DBEROU010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.034"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(109..2367)
                     /locus_tag="ACL8CL_001481"
     CDS             complement(109..2367)
                     /locus_tag="ACL8CL_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS1330711.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGFA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKAEVKSSEKEK"
     gene            complement(2596..2826)
                     /locus_tag="ACL8CL_001482"
     CDS             complement(2596..2826)
                     /locus_tag="ACL8CL_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1797 family protein"
                     /protein_id="HGS1330712.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            3016..3699
                     /locus_tag="ACL8CL_001483"
     CDS             3016..3699
                     /locus_tag="ACL8CL_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HGS1330713.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQVGILGVDLSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KRMGQGDKK"
     gene            3699..4433
                     /locus_tag="ACL8CL_001484"
     CDS             3699..4433
                     /locus_tag="ACL8CL_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1330714.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKVIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESKAVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMEPD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4587..5444
                     /locus_tag="ACL8CL_001485"
     CDS             4587..5444
                     /locus_tag="ACL8CL_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HGS1330715.1"
                     /translation="MTQIIDGKALAAKLQGQLAEKTEKLKEETGLVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGFRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLTHSRTHNLSKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5587..6459
                     /locus_tag="ACL8CL_001486"
     CDS             5587..6459
                     /locus_tag="ACL8CL_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HGS1330716.1"
                     /translation="MKVINQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPDLHSHLPEPMAFSLQDQQLLKEQLEKAEVILLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLSKIMK
                     IICQQDRVSKDKLV"
     gene            6741..7424
                     /gene="rpiA"
                     /locus_tag="ACL8CL_001487"
     CDS             6741..7424
                     /gene="rpiA"
                     /locus_tag="ACL8CL_001487"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HGS1330717.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7438..8649
                     /locus_tag="ACL8CL_001488"
     CDS             7438..8649
                     /locus_tag="ACL8CL_001488"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019318858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HGS1330718.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAESNPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8651..9193
                     /locus_tag="ACL8CL_001489"
     CDS             8651..9193
                     /locus_tag="ACL8CL_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HGS1330719.1"
                     /translation="MTRYALLVRGINVGGKNKVVMAELRQELTNLGLEKVESYINSGN
                     IFFTSIDSKAQLVEKLETFFAVYYPFIQSFSLLSLEDFEAELENLPAWWSRDLARKDF
                     LFYTEGLDVDQVIATVESLELKDEVLYFGKLGIFWGKFSEESYSKTAYHKYLLKVPFY
                     RHITIRNAKTFDKIGQMLKK"
     gene            9209..10018
                     /locus_tag="ACL8CL_001490"
     CDS             9209..10018
                     /locus_tag="ACL8CL_001490"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGS1330720.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10508..10903
                     /locus_tag="ACL8CL_001491"
     CDS             10508..10903
                     /locus_tag="ACL8CL_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HGS1330721.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEITEPAQADDIRKVVGELAKDISITKLYMTGHSLGGYLAQIAAVEDYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            10913..11488
                     /locus_tag="ACL8CL_001492"
     CDS             10913..11488
                     /locus_tag="ACL8CL_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HGS1330722.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11513..12025
                     /locus_tag="ACL8CL_001493"
     CDS             11513..12025
                     /locus_tag="ACL8CL_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-activated cytolysin C-terminal
                     domain-containing protein"
                     /protein_id="HGS1330723.1"
                     /translation="MSAQITINHKKARYVRIELEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVPVDKDGNQVVRSHSWEGNGC
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12681..13391
                     /gene="deoD"
                     /locus_tag="ACL8CL_001494"
     CDS             12681..13391
                     /gene="deoD"
                     /locus_tag="ACL8CL_001494"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HGS1330724.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13414..13938)
                     /locus_tag="ACL8CL_001495"
     CDS             complement(13414..13938)
                     /locus_tag="ACL8CL_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HGS1330725.1"
                     /translation="MKIAIIGYSGSGKSTIAEKLSNYYSIPKLHMDTLQFQPGLQDSD
                     CEWMLTEIKNFLTKYKAWVIDGNYSWCYYQERMQEADQIIFLNFSPLTCLFRAFKRYL
                     KYRGKVRESMAVDCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(13991..14227)
                     /gene="rpsT"
                     /locus_tag="ACL8CL_001496"
     CDS             complement(13991..14227)
                     /gene="rpsT"
                     /locus_tag="ACL8CL_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HGS1330726.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLVK"
     gene            complement(14295..15215)
                     /gene="coaA"
                     /locus_tag="ACL8CL_001497"
     CDS             complement(14295..15215)
                     /gene="coaA"
                     /locus_tag="ACL8CL_001497"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HGS1330727.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
     gene            15405..15808
                     /locus_tag="ACL8CL_001498"
                     /pseudo
     CDS             15405..15808
                     /locus_tag="ACL8CL_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16015..16605
                     /locus_tag="ACL8CL_001499"
     CDS             16015..16605
                     /locus_tag="ACL8CL_001499"
                     /EC_number="2.1.1.172"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0052916 - 23S rRNA
                     (guanine(1835)-N(2))-methyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HGS1330728.1"
                     /translation="MSKMYYAENPDAAHDIHELRVDLLGEKMTFLTDAGVFSKKMVDF
                     GSQLLLKCLDVNQGETVLDVGCGYGPLGLSLVKVYGVQATMVDINTRALDLAQRNAEK
                     NNAKATIFQSNIYARVQGRFDHVISNPPIRAGKQVVHEIIEKSKDFLEIGGDLTIVIQ
                     KKQGAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            16602..17879
                     /locus_tag="ACL8CL_001500"
     CDS             16602..17879
                     /locus_tag="ACL8CL_001500"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016154 - pyrimidine-nucleoside
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HGS1330729.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            17897..18559
                     /gene="deoC"
                     /locus_tag="ACL8CL_001501"
     CDS             17897..18559
                     /gene="deoC"
                     /locus_tag="ACL8CL_001501"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HGS1330730.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYDFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGDLKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKIVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            18546..18935
                     /locus_tag="ACL8CL_001502"
     CDS             18546..18935
                     /locus_tag="ACL8CL_001502"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGS1330731.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NVSYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            19026..20078
                     /locus_tag="ACL8CL_001503"
     CDS             19026..20078
                     /locus_tag="ACL8CL_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HGS1330732.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVEEAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDIANKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            20222..21757
                     /locus_tag="ACL8CL_001504"
     CDS             20222..21757
                     /locus_tag="ACL8CL_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330733.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            21750..22808
                     /locus_tag="ACL8CL_001505"
     CDS             21750..22808
                     /locus_tag="ACL8CL_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1330734.1"
                     /translation="MSKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            22811..23767
                     /locus_tag="ACL8CL_001506"
     CDS             22811..23767
                     /locus_tag="ACL8CL_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1330735.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         6918 a         4310 c         5406 g         7160 t
ORIGIN      
        1 gtctactata tatcataatg gacaaagggc aagctgtcaa gaaattacag ttattttagt
       61 aaaaaaagaa gcagaaaaat ttcattttct actccttttc tataggattt atttttcttt
      121 ttcagaactt ttaacttcag cttttttggc agatttaatc tgaatgtttc ccttgctagt
      181 cataactgct ttgagatctt tttcgcttgg attttcaagg tagtagtcag tgattgtgtc
      241 ctcaatatag tcttgaatag tccgacgaag tgggcgtgct cccatttttg gatcataacc
      301 taggtcaacc aacttttcct tgaccttgtc agttacatcc aaacgaatgt tgttgctaga
      361 gaggcgcttg ttaacatctg ctagcatgag ctcgacaatc tgaaggaggt tatccttgct
      421 gagagcctta aattcgataa tgccatcaaa acggttcata aactctgggc taaagaagtt
      481 accgagttca ccgaggacag aattggtacg tccttctcta gcagcaccaa atccaacgct
      541 agcttcggtc tttcctgtac ctgcatttga ggtcatgata atgatggcat ccttgaagct
      601 aacggtgcgt ccttgcccgt ctgtcaaacg accatcgtcc aagacttgaa gaaacatgtg
      661 cataacatct gggtgagctt tttccacttc atcgagaagg atgagagaat atggattgtg
      721 gcgaactttt tcagttaatt gaccagcctc atcatagcca acataacctg gaggggcgcc
      781 gaccaactta gccacactat gtttttccat gtattcactc atatcaaagc gaatcatact
      841 atcagcaaaa ccaaaaagtt cgatagccag ttgtttggaa agttctgtct taccgacacc
      901 agttggccca acgaagagga agcttccgat tgggcggtta ggggtaccaa gtccgacacg
      961 attacggcga atagccttgg caatcttatc gactgcatca tcttggccaa taacatgaga
     1021 cttgagatct tcggctagat ggatgagttg agattgttct ttctctttca aatcaccaac
     1081 agggatattg gttttctgct cgataatgtg ctcaatagtt ttctcgctga tgataggagt
     1141 atcctggtct gtgatctttt tcttttgcat ttccttatac ttggcaatct ggtcgcggaa
     1201 gtaggccgcc ttctcaaaat cttcttctcg tgtagcttga gacttgagat tttcagcctc
     1261 aatcaagcgc tgatcaatta ctttaggatc cacaaaattc aaggtcaagt tcatcttaga
     1321 accagcttca tctaggaggt caatggcctt gtcaggcaag aagcgatctt ggatgtagcg
     1381 attggaaaga gttgcagctg cttcaatcgc agcatctgta tattgaacgt ggtggtaatc
     1441 ttcgtatttc ttttgaatcc ctttgagaat agtgattgtt tcgtccaccg ttggttcatc
     1501 gactttaaca ggctgcatac gacgctcgag ggcagcatcc ttttcaatga tacggtattc
     1561 attgagggta gtagcaccga ctagttgcag ttctccacga gcaagggctg gcttgaggat
     1621 atttcctgcg tccatattac catcactcgc agaaccagca ccaacaattt catggatttc
     1681 atcgataaag aggatgatgt cttcacgttt gcgaatttct tccatgagtt tttgcatgcg
     1741 ttcttcaaat tgtcctcgaa tccccgttcc ttgaactaag ctaaccacat ccagacggat
     1801 gacttgttta ccttggagtt tatgtggcac atcgccatcg acaattttct gagctagacc
     1861 ttcgacaacg gccgtttttc cgacaccagg ttcaccgata aggacaggat tattcttggt
     1921 tctacgattg agaatctcga tgacacggat aatctcatcg tcgcgcccaa taacggggtc
     1981 aatgtctcca cgacgggcaa tttcagttac attaatacca aattcttcca gcaggccttt
     2041 ttcttggcta ggtggcggag tttgagcaga tccacgattt tgggaaccat aaccgccgtt
     2101 tccaccgtaa cctccacctg attgggttgg gggaatagga ggagtattgc tagaaggtct
     2161 gaaattgttt agatcattga agaaatcacc aaagggatcg aagtcacgat tgttcagatc
     2221 cgtcatacct ttgaagaggc tattgttagg atctgttttg ataatcttat agcagttttg
     2281 acagaggtca atttgttttt gttttccatt gagattggtg taaagatgaa ttgttgagtc
     2341 gttaatttta cagttttgac aaagcataca tctacctcat ttctttcttt agcctgactt
     2401 gtttaaaggt caaaaatagt caaattctat actctcatta taacaggatt ggggtgtaaa
     2461 gcaagtaaaa agattgaagc tttgagaagt tgttacaatg aaaatttttg tgaatttgga
     2521 ctataaaaaa atcctatttg tgagacaaaa aggattttac ttagacatgc agggacaatc
     2581 ccagcttgtt ttagattagt agagaagttc gtaaatttcc attgcgatta agtcaatgct
     2641 atcaaacgta tacgtttcgc gatcttctac gtcacgaagg gtaaagattg ggccgttttc
     2701 ttcgtcgtgg ctgtacgcaa cttctgcaac aacaactcct tcgcgttcaa aattacgttt
     2761 taaatttccg ccatcgtttg ccattgcttc aaggcggttg atgattctaa ccaaatgtga
     2821 ttccattttg attctccttc atttcttatc gttactattt taccataaag gaggccaact
     2881 tgactagaaa aaactaagat ttctcgctaa ttctgctagt ttaaagattt ttaaataaat
     2941 tggataaaaa atttaaattt taattcaaaa catgttataa tcatacagta ttctatttta
     3001 gaaagcagtg tgactatgaa tttttctttt ttacctaagt atttacctta ttttaactat
     3061 ggggctgttg tgacgattct tatttctatc tgtgttatct ttttgggaac tattttgggt
     3121 gttgtcttgg cttttgggca acgttcaaag tttaaaccgc ttgtttggtt ggccaacttg
     3181 tacgtttgga ttttccgtgg gacaccgatg atggttcaaa ttatgattgc ctttgctctt
     3241 atgcatatca atgctccgac tattcaggtt ggaattttag gtgttgatct ttctcgtctg
     3301 attccaggga ttttgattat ctctatgaac agtggtgctt atgtttctga gactgttcgt
     3361 gccggaatca atgcagttcc taaggggcag ttagaggcgg cttattcttt agggattcgt
     3421 cctaaaaatg ctatgcgcta tgtaatttta ccacaagcta tcaaaaatat cttgccagct
     3481 ttggggaacg aatttatcac cattatcaag gacagctccc tcttatcagc tatcggtgtt
     3541 atggagttgt ggaacggggc tacaacagtt tctacaacaa cctatctacc tttaacacca
     3601 cttttatttg cagcttttta ttacttgatt atgacttcta ttttgacagt agccttgaaa
     3661 gcttttgaaa aacgtatggg acaaggagat aagaaataat gacagaaacc ttgataaaaa
     3721 ttgaaaattt acataaatca tttggaaaga acgaagtatt gaagggcatc aacctcgaga
     3781 ttaaaagagg agaagttgtc gttatcatcg gtccttcagg gagcgggaaa tctaccttgc
     3841 ttcgctctat gaatttgttg gaagaagcga ccaagggtaa ggttatcttt gagggagtcg
     3901 atattacgga caagaagaat gacctctttg ccatgcgtga gaagatgggc atggttttcc
     3961 aacaatttaa cctctttcct aatatgactg tgatggaaaa tatcaccttg tcacctatca
     4021 agaccaaagg tgagagcaag gctgttgctg agaagagagc ccaagagctt ttggaaaaag
     4081 ttggtttgcc agataaggca gatgcttatc cacagagttt gtcaggtggt cagcaacaac
     4141 ggattgccat tgcgcgtggg ttggccatgg aacctgatgt tttgctcttt gacgagccaa
     4201 cttcagcact agatcctgag atggttggag aagttctggc tgttatgcaa gatctagcca
     4261 agtcaggaat gaccatggtt atcgtaacac atgagatggg atttgcccgt gaggtggcag
     4321 atcgtgtcat ctttatggca gacggtgtgg ttgttgaaga cggaacacca gaacagattt
     4381 ttgaacaaac ccaagaacaa cggactaaag acttcttgag taaggtttta taatctattt
     4441 taaaatttca agacaagatt agtgaaaagc tcaaccagag ctttttctta tagtttgaaa
     4501 ctataggatt gcctaggaaa gaagtgttag agggtgctgg cagtttttgg tataatggaa
     4561 gatatttgaa agaaaagaga agtgatatga cacagattat tgatggaaaa gctttagcgg
     4621 ccaaattgca ggggcagttg gctgaaaaga ctgaaaaatt aaaggaagaa acaggtctag
     4681 tgcctggttt ggtagtgatt ttggttgggg acaatccagc cagccaagtc tacgttcgca
     4741 acaaggagag gtcagctctt gcagctggtt tccgtagcga agtagtgcga gttccagaga
     4801 ccattactca agaggaattg ttagacctga ttgctaaata taatcaggat ccagcttggc
     4861 atgggatttt ggtccagttg ccattaccaa aacatatcga tgaagaggcg gttttattag
     4921 ccattgaccc agaaaaggat gtggatggtt tccatcccct aaacatggga cgtctttggt
     4981 ctggacatcc agtcatgatt ccctcgacac ctgcaggaat tatggaaatg ttccatgaat
     5041 atgggattga cttggaaggt aaaaatgcag tcgtcatcgg tcgatccaat attgtcggaa
     5101 aacctatggc ccagcttctt ttggcaaaga atgcaacagt aaccttgact cactcacgta
     5161 ctcataatct ttccaaggtg gctgcaaaag cagatattct ggttgttgca atcggccgcg
     5221 ccaagtttgt gactgctgac tttgtcaaac caggtgcggt agtcattgac gttgggatga
     5281 accgcgatga aaatggtaag ctctgtgggg atgttgatta tgaggcggtt gccccacttg
     5341 ctagccacat tacgccagtc cctggaggtg tcggtcctat gaccattact atgctgatgg
     5401 agcaaaccta tcaggcagca cttaggacat tggatagaaa ataagagaaa cattcgatta
     5461 atagagtgta ttttctatag ctatatctaa caatggcaga aatgaatatt aaattttaga
     5521 tataagatta caaaaggagg tctgcgtctc ctttttattg tataataaaa gtgagaggaa
     5581 aaaaggatga aagtgattaa tcaaacctta ttagaaaaag ttattattga acgttctcgt
     5641 tcaagtcata aaggagatta tggtcgtctg ctgctgcttg gtggtactta tccttatgga
     5701 ggtgccatca tcatggctgc tttagcagct gtaaaaagcg gtgcaggatt ggtgaccgtt
     5761 ggaacggaca gggaaaatat cccggatctg cacagccatt tacctgagcc tatggccttt
     5821 tctcttcaag accagcaatt gttaaaagag caattggaga aggcagaagt tatcttgctg
     5881 gggcctggtt taggagacaa tgcttttgga gaagatttag ttaaacaggt ctttgctggc
     5941 ttaaaacaga atcagatttt gattgtagac ggaggtgcct tgaccattct tgctaggaca
     6001 agtttgtcat ttccctcaag ccagcttatc ctaactcctc accaaaaaga atgggaaaaa
     6061 ttatctggaa ttacgattga aaagcaaaag gaagatgcaa cggctagtgt tctgacttcc
     6121 ttccctcaag gaacaatttt ggtggagaaa ggcccagcta ctcgtatttg ggaagttggc
     6181 caatctgatt attaccagtt acaggttggt ggtccttatc aggcgactgg gggaatggga
     6241 gatactctgg ctgggatgat cgcaggattt gtaggccaat tccgacaggc cagtctctac
     6301 gagcgtgtgg cagtagcgac ccatcttcat tcagccatag cccaagaact atctcaagaa
     6361 aattatgtgg tcttaccgac ggaaatcagc aggtatcttt ccaaaataat gaaaattatt
     6421 tgtcaacaag atagggtaag caaagataaa cttgtttaaa atagacagat aaatctactt
     6481 ccttctctta atttatgatt cttgaaatgc gcctgcttta aggctaaact agacgtatca
     6541 aagttaagag tttgtttttg taactggaaa gagatgaaca aatcaaatac ataaaagata
     6601 aactcatttt tattcgtttg gtaaaaataa aatgcatatt tttaaagaaa gattgatgga
     6661 aaatgtagat ttgatgtttt tacttgaata aaaagcggtt tcatagtaaa ataagaactg
     6721 agaatgatgg aggtaaatcg gtggaaaatc tgaagaaaat ggcaggtatc aaggctgctg
     6781 agttcgtgag cgatggaatg gtcgttggac ttggaacagg ctcgactgcc tattattttg
     6841 tcgaagaaat cggtcgtcgt atcaaggaag aaggattgca gattacagct gtaacaactt
     6901 ctagtgtgac cactaaacag gcagaaggcc ttaatatccc gctcaagtct attgaccaag
     6961 tagactttgt cgatgtgacg gttgatggcg cggatgaagt ggatagtcag tttaatggaa
     7021 tcaaaggcgg tggtggtgcc cttctcatgg aaaaggtggt cgcaacacca tcaaaagaat
     7081 acatttgggt ggtggatgaa agcaagctgg tcgaaaaact aggtgctttt aaattgccag
     7141 tagaagtggt tcagtatggt gcagagcagg tctttcgtca ttttgaacga gctggctaca
     7201 aaccaagttt ccgtgaaaaa gacggccaac gttttgtgac ggatatgcag aattttatca
     7261 ttgacctcgc cttggatgtc attgaaaatc caattgcttt tggacaagaa ttggaccatg
     7321 tcgttggtgt tgtggagcat ggtttattca accaaatggt ggataaggta atcgttgctg
     7381 gacgagatgg agttcagatt tcaacttcaa aaaaaggaaa atagaagggg gcataagatg
     7441 tctaaattta atcgtattca tttggtggta ctggattctg taggaatcgg tgcagcacca
     7501 gatgctaata actttgtcaa tgcaggggtt ccagatggag cttctgacac actgggacac
     7561 atttcaaaaa cagttggttt gaatgtccca aacatggcta aaataggtct tggaaatatt
     7621 cctcgtgaaa ctcctcttaa gactgtagca gctgaaagca atccaactgg atatgcaaca
     7681 aaattagagg aagtatctct tggtaaggat actatgactg gacactggga aatcatggga
     7741 ctcaacatta ctgagccttt cgatactttc tggaacggat tcccagaaga aatcctgaca
     7801 aaaatcgaag aattctcagg acgcaaggtt attcgtgaag ccaacaaacc ttattcagga
     7861 acggctgtta tcgatgattt tggaccacgt cagatggaaa ctggagagtt gattatctat
     7921 acttcagctg accctgtttt gcagattgct gcccacgaag acattattcc tttggatgaa
     7981 ttgtaccgta tctgtgaata cgctcgttcg attacccttg agcgtcctgc ccttcttggt
     8041 cgcatcattg ctcgccctta tgtaggtgaa ccaggtaact tcactcgtac ggcaaaccgt
     8101 cgtgacttgg ctgtatctcc attttcccca actgttttgg ataaattgaa tgaggctggt
     8161 atcgatactt atgctgtggg taaaatcaac gatatcttta acggtgctgg tatcaaccat
     8221 gacatgggtc acaacaagtc aaatagtcat ggaattgata cactattgaa gactatggga
     8281 cttgctgagt ttgaaaaagg attctcattc acaaacctag ttgactttga tgccctttac
     8341 ggccatcgtc gtaatgctca cggttaccgt gattgcttgc atgagtttga tgaacgctta
     8401 cctgaaatta tcgcagctat gagagagaat gaccttctct tgattactgc ggaccatgga
     8461 aatgacccaa cgtatgcagg aacggatcac actcgggaat atattccatt gttggcctat
     8521 agccctgcct ttaaaggaaa tggtctcatt ccagtaggac attttgcaga tatttcagcg
     8581 actgttgccg ataactttgg tgtggaaact gctatgattg gggaaagttt cttagataaa
     8641 ttggtataag atgacgcgct atgctttgct ggtgagaggt atcaatgttg gtggtaagaa
     8701 taaggtcgtc atggcggagc ttcgtcaaga attgacaaac ttgggactgg aaaaggttga
     8761 gagctacatc aacagtggca atattttctt tacttcgata gattccaaag cccaattggt
     8821 tgaaaagcta gagactttct ttgcagtcta ttatccattt attcagagct tttctttact
     8881 gagtctagag gactttgagg cggaacttga aaatctacca gcttggtgga gcagagactt
     8941 ggcacgaaaa gattttctct tttacactga gggtttggat gtggaccaag tcatcgcgac
     9001 agttgaaagt ttagagctga aagatgaagt gctttatttt ggaaaacttg ggattttctg
     9061 ggggaaattt tctgaagaat cctattctaa gactgcctat cataagtact tgctgaaggt
     9121 gcctttctac cgccacatta ctattcgtaa tgctaaaacc tttgacaaaa ttggtcaaat
     9181 gctaaaaaaa taataaagga gacacacaat gacattttta aacaaaatcc atgaaactgc
     9241 tactttcctg aaagaaaagg gaattgcagc ccctgagttc ggtctaatcc ttggatcagg
     9301 acttggagaa ttggcagaag aaatcgaaaa tccagttgta gtagactatg ctgagattcc
     9361 aaactggggc cgttcaacag tagtcggtca tgctggtaaa ttggtatatg gtgaactggc
     9421 aggtcgcaag gtcttggctc ttcaagggcg tttccatttc tatgaaggga atcctctgga
     9481 agtggtgact ttcccagttc gtgtgatgaa agttcttgga tgtgaaggtg ttattgtaac
     9541 caatgcagct ggcggtatcg gatttggtcc tggtaccttg atggctatct cagaccatat
     9601 caacatgacg gggcaaaatc cattgatggg tgaaaacttg gatgactttg gcccacgttt
     9661 cccagatatg tctagggctt acacaccaga ataccgtgcc actgcccatg aagtggctaa
     9721 aaaacttaat atcaagcttg atgaaggtgt ctatatcgga gttactggtc cgacttatga
     9781 aacaccagca gaaattcgtt cctataagac actgggagca gatgcagttg gtatgtctac
     9841 ggttcctgaa gttatcgtgg cagcccactc tggcttgaaa gttctgggaa tttcatgtat
     9901 cactaacttt gcggccggtt tccaagaaga actcaatcac gaagaagttg tagaagtgac
     9961 tgaacgtgtt aaaggtgatt tcaaaggctt gcttaaagcg attcttgctg aattgtaaga
    10021 aaaaagattt aaaaggggga gtgcctctgt cttttcagga ttgactgcct atccgtatta
    10081 aagaagaaac agaggaatac tatgagcttc ttcctgctct tataactgaa agaagcggaa
    10141 gaataggtat gtctgatctg atagccagca ttgtgaagga caagattcta ggatactagc
    10201 attagcttcc tagcattagc ttcctagcca agcagactag tatgataagg agagatgaga
    10261 atgaattgac tttctgaatt tctcagtctt atcatatata gcacaatgag atttcgcttg
    10321 agtctgcttg taaataaacg aaaagaaaga taagaaataa tgaaaattgg tcaacgaatt
    10381 atgcgctttg gcataaaaaa ttaagtatcg gagttgtatc tgttgtagtc ggctttgatt
    10441 tctagctcca gctggaattt cagccaatga agtaaagcaa gatgtaacat ctgaagtggt
    10501 aataggtgtg ctagattcta aggaggaatt gaaagagtca gaaaatgatg ctccaaaact
    10561 agaaactcct cttagagagg agccaagact agctcctcaa acgcttccgg aagcaagtga
    10621 agttcttgaa aacaaaaggg aagagtcaaa agtagagata acagagccag ctcaagcgga
    10681 tgatatccgc aaggttgttg gggaattagc caaggatata agtattacta agttgtatat
    10741 gacaggtcat tctcttggag gctacctagc tcagattgca gcggttgaag attaccaaaa
    10801 atatcctgat ttttataacc atgtattgag gaaagtgaca actttcagtg ctcctaaagt
    10861 gattacttcc agaactgttt gggatgctaa gaatggtttc tgagatgttg gtttggaaag
    10921 tcgtaaatta gctgttagtg gaaaaattaa gcattatgtg gttgataatg acaatgttgt
    10981 gactcccttg attcataata atcgtgatat tgttacattt acaggtaatt cacgctttaa
    11041 acaccgttct cgtggctatt ttgaaagtcc aatgaatgat attcctaact ttaatattgg
    11101 taaacaagct accttggata aacatggtta tcgtgatccg aaattggata aagtgcgatt
    11161 ctttaagaaa caggctctac ctcaatcttc tagtcaacca agcgctgaac caatggaaaa
    11221 tattgcctta ggaaaacagg ttactcaaag ttcgacagct ttcggaggag atgctagaag
    11281 agctgtggat ggcaaagtcg atggtaacta tggtcacaat tctgtcactc atacaaactt
    11341 ccaatctaag ccttggtggc aagtagattt ggctaaagaa gaaaccattc gccaaatcaa
    11401 tatttacaac cgaacagaca ctgcccagga tagattggca aactttgatg tcattctttt
    11461 agacagttct ggtaaagaaa ttgagtgaaa acgtataata tctcctaaag atgtgtcagc
    11521 acaaattacg attaaccata aaaaagcgcg ctatgttcgg attgagctag aaggctataa
    11581 tgccctcagt cttgcagaag ttgaagtttt ccgctttata gctacgaatg ctgaaacggc
    11641 gacacaagtt tctaagccag ttcaaccaat cagtcagact cctgtgaaag ataaaacatt
    11701 gacaattcaa cacagtggag cttacattgc ccgctactcc ataacttggg aagaagttcc
    11761 agtagataaa gatggaaacc aagttgttcg tagtcattct tgggaaggaa acggttgcaa
    11821 ccagactgca ggttttgtcc tcaacctccc aatcaaagaa aatatgagaa atctgcgagt
    11881 taagattgag aaaaagacgg gcctactatg gaatagatgg caaacaatct atgaaaacag
    11941 accaatttta gctcaacccc accgtaaaat tacccattgg ggtacgacat tgaattccaa
    12001 ggtgagtgac gatgatgtct tgtaatctga tggtacaatg acagttagtt tgtctagttt
    12061 ataagaaagt actacctgag cttgaataga actcaggtag ctctatgaaa gaacaaaatt
    12121 aatactcaat gaaaatcaaa gagcaaacta ggaaactagc cgcaggttgc tcaaagcact
    12181 gctttgaggt tgtagataag actgacgaag tcagtcacat atataatcca aggcgacgtt
    12241 gacgtggttt gaagagattt tcgaagagta taaacagaaa ggtagagcgc gtgttctaat
    12301 ttgaacacga gtagaaaact tttctaaaaa caaaaacgaa aggatgggta aactgtattc
    12361 gctgaactga atacgggcga ctctcctcta aatcaaaatt aagaaagaaa ggaattgacc
    12421 ccaccctaaa agcagtggga aaaagatagt tgatctagcg agcatcgctc actgcgccca
    12481 actcctattt tcccttcgct ttttgatggg tttggtatct ttctcaatat aaaatataaa
    12541 ataaagaaag gtagagcgtg tgttttgatt tgaacacgag cggaaaactc ggaaaataga
    12601 taatctgact gaaaaatcag gatttctcgt caggttccta attttcagtc gttttcttct
    12661 cgctctttgt atcataaatt atgtctatcc atattgctgc tcagcagggt gaaattgctg
    12721 ataaaattct tcttcctggg gatcctcttc gtgctaagtt tattgcggag aatttccttg
    12781 atgatgctgt ttgttttaac gaagtgcgta acatgtttgg ttacactggt acttacaagg
    12841 gtcactgtgt atctgtcatg ggaactggga tgggaatgcc atctatttcg atttatgcgc
    12901 gtgagttaat cgtagactac ggtgtgaaga aattgattcg tgtgggaact gcaggttctt
    12961 tgaatgaaga ggttcatgtt cgtgaattag ttttggcgca ggcggctgca accaactcaa
    13021 acatcgttcg taatgactgg ccacagtacg attttccaca aattgctagc tttgatttgc
    13081 ttgataaagc ctaccatatc gccaaaaaac ttggtatgac tactcacgtt gggaacgttt
    13141 tgtcatctga tgtcttttac tcaaattact ttgaaaagaa tatcgagctt ggtaaatggg
    13201 gagtcaaggc tgtggaaatg gaagcagcag ctctttacta tcttgctgcc caataccatg
    13261 ttgatgcgct agctatcatg accatctctg atagcttggt caatccagac gaagacacaa
    13321 ctgcagaaga acgtcaaaat accttcactg atatgatgaa ggttggtttg gaaaccttga
    13381 ttgcagaata attatagcca aaaaggaagc cctttatgaa ttgtaggact tccttttctt
    13441 atccagaaat tgatctagct ctctctgatt tcgaagaata gtgactttat gtgaatattc
    13501 ttggcaaagt ttttggtaat tttctttttg agttttgcta cgcccatccc aaagaatcca
    13561 tctgataaac tcccagtcaa agcgttcagg gcaatctacc gccatacttt ctctgacttt
    13621 tccacggtat ttaagataac gcttaaaggc tctaaagaga caggtcaatg gcgaaaaatt
    13681 gagaaagatg atttggtcag cttcttgcat tcgttcttgg tagtagcacc aagaataatt
    13741 accatcgatg acccaagctt tatacttggt gagaaagttt tttatctcgg ttaacatcca
    13801 ttcgcagtca ctgtcttgca aaccaggttg aaattggagt gtgtccatgt gcagttttgg
    13861 aatggagtag tagttagata acttttctgc tatagttgac ttaccagaac cagaatatcc
    13921 gataattgcg attttcattt tctacctttt cctatttgga gacaaaaaaa cagcctctat
    13981 ggactgtttc ttatttaaca agtttagctg aaagacgagc tttatcgcgg cttgctttgt
    14041 ttttgtgaat caaaccttta gtttctgctt tatcgatagc tgagctagca gcacggaaaa
    14101 gttcttcaga tgggtttgct tcgaaagctt ttatagcagt acgcatagct gatttttgag
    14161 ctgagttctt ttcgttttgt ttaacgttca attcagcgcg tttgatagct gatttaatgt
    14221 ttgccaatgt tcttacctcc atatttacta actataccat tatatatgaa aacttacgtt
    14281 ttgacaaggg aaaattactt ttttaagtaa atttcatcga tttcatggtt cttgctttta
    14341 tgaagaatca cttctgcacg atttctggtt ggttcaatat aattttgcag atttgtgaga
    14401 ttgatactga tccagacctg atgggcaaag gactccactt ccccaatcgg catctgagta
    14461 aaacgataat agtagctatc agggtcgttt tgggctagac tcagcatttt caagaaacgg
    14521 tccagatacc aactttcaat atcatccact ccagcatcta catagatgga aaagtcaaag
    14581 aagtcagtga tatagagacg atcgttttgt ggattttgaa agacattgat tccctcaaca
    14641 attacaaaat cagcagcttt gacactttgt ttctcttcgg gtacgatgtc gtaaacttca
    14701 tgagaataga caggaatatc tacatcttgt ccatttttga tgcggtccaa gaagttgaga
    14761 agagcttcca tatcatagct ttcaggaaat cctttacgat ttaaaatccc ctgctcaatc
    14821 aaggtttgat tgggatagag aaaaccatca gttgtaacca actcaaccgt agcatctgta
    14881 aacgtacggg acagtaggat ttgaagtagg cgactggttg tggattttcc aacggcaaca
    14941 ctcccagaaa ccccaataat aaaaggttga gatttacttt cacgttggag gaaaattcct
    15001 tttgaaaagg ctaaatcttc cttagttcgc ttgtaaatct gaatcaaatg agccaagggg
    15061 agatagatat ctgtaacgtc ttggagactg atttggtcat taaaactctt gatagattcc
    15121 aattcttctt ctgtcaaagg aggtgttgtc tttcgatgta aagattgcca agtctggcgg
    15181 ctgatttttt caaaatgtaa aaattcgttg gtcatttgtt ttcccctaga tattttgtct
    15241 atcataccat aaaaagctaa aaaaataaag cggtttcttg aatgtggtaa gtcaatggta
    15301 tatagtataa gtgatcaatt tattgatgaa ataggagata gattctactg atttataagc
    15361 ttataaaggg ggctttttag ttcacaactt ataggaggtg tactatgaaa atcttaaaac
    15421 gttacatatt ggaactctgt tttattttaa gttttgcttt accttttata aaaggaacca
    15481 atgcagataa tggtagatgc tttgtggaaa cctattacgg ttttactttt ttgatggaac
    15541 atgctattgt aacagctgtc tttatttgtt cgttcttaat tgctttctta ctaaaaaacg
    15601 atggacgaaa tggattgctg cgggtagtta ttgcttttta gtcctacgga ttgcgacaga
    15661 gggttatttc ttccacatgt cactagaaga tttgatacga ctttggacaa gtttggaaat
    15721 cctgacacaa acgtatcagt tgggctttta tctaaacatt ttgttaggaa ttttgttgat
    15781 aataaagtat tttaaggtta agcaatagaa ataataatct gcctgattat agatgataaa
    15841 ctttttgttt ttatctggac tgtgatattt atagttctaa atggtttatt atttataagt
    15901 gttgaaagag attgaagaaa atggcttcgt gacttgcttt cttcggtctt tttcgtttta
    15961 ttatcaattt gtaaacgatt tacaatttga gtcaaattat ggtaaaatag tgtcatgagt
    16021 aaaatgtact atgcagaaaa tcctgacgct gctcacgaca ttcatgagtt gagagtggac
    16081 ttgttgggag aaaaaatgac ttttttgaca gatgcgggtg tttttagcaa gaaaatggtt
    16141 gactttggaa gtcaactctt gctcaagtgt ctggatgtca accaaggaga gacagtccta
    16201 gatgtaggct gtggttatgg tccattaggt ttgtccttgg tcaaggttta cggagttcag
    16261 gcgaccatgg ttgatattaa cactcgtgcc ctggatttag ctcagagaaa tgctgaaaaa
    16321 aataatgcaa aagcgacgat atttcaatct aatatttatg caagagttca agggcgattt
    16381 gaccatgtca tttccaatcc tcctatccgt gcgggcaagc aagtcgttca tgaaatcatc
    16441 gagaaaagta aagatttctt ggaaattggt ggagatttga ccatcgtcat ccagaaaaaa
    16501 caaggagctc caagtgccaa gtctaagatg gaagatgtgt ttggcaattg tgaaatcctt
    16561 aaaaaagaca agggatacta tatccttaga agtgtgaaag aatgagagca gttgatttaa
    16621 tccaaaaaaa acgagacggt caagaactga cttcaagtga aattgaatgg ctgatagaag
    16681 gctatgtatc aggaactgtt cctgattatc agatgtctgc ctttgctatg gctgtttatt
    16741 ttaaaggaat gacgactcga gaaatctctg atttaacgat gaatatggtt aagactgggc
    16801 aagagtttga cctgtcagcc attgatggtg tgaaagttga caagcattct actggtggtg
    16861 tcggcgataa ggtaaccttg attttagctc ctcttgttgc tagcttcggt gtgcctgtag
    16921 caaaaatgag tggtcgtggt cttggccata caggcggaac aattgataaa ttggagtcca
    16981 ttaagggcta tcaagtagaa cgtagtcaag aggattttat tcgtcaggtt caggatattg
    17041 gagtatctgt cattgggcaa tcagaccagc tggtcaaagc agataagctt ctctatgccc
    17101 ttcgtgatgt gaccgcaact gtcgacacga ttcctttgat tgcgagttcg gtgatgagca
    17161 agaaaattgc ggcaggggcg gatgctattt tgctagacgt gactgtcggt gagggtgcct
    17221 tcatgaagac ggttgatgag gcgcgtgagt tggctcaaac tatggtagat cttggtaagg
    17281 ttgttggacg gaagacagta gcagtgatta cggatatgag ccagcctttg ggacgagcga
    17341 ttggaaatcg tctggaaatc cttgaagcat tggagatttt gcaaggtcaa ggacgtcagg
    17401 atattaccca ctttatttgt gaattggctc aaattatgct tggtttggca aatgttaaca
    17461 aaactgttga agaagttcgc caacatcttg aaaatggtca agcactggct aagtttgagg
    17521 aaatggtgca agcccaaggt ggagacttgg aagacctcta tcgtcctgta aatgtagctc
    17581 atgtggtgga aatccctgct caggaaacgg gtgtcatttc agctcttcca gctatggact
    17641 ttggtcttta tgccatgagg ctgggtgctg gtcgtgcagt taagtctgat gccttggact
    17701 atgaaacagg aattgttttt gaaaagaaga ttggagactc cgttcaaaag ggtgaaattg
    17761 ttgcaaaagt atacacaaat gaaaaaattc cacctcaact agttacagat ttccaaaaat
    17821 gtgttaaaat aagtgatgaa gtgaaaaaaa tccgtgaaat tgtagaaatc atctcctaaa
    17881 tggagggaaa accgaaatga aattaaataa atatatagat catacgcttt taaaacaaga
    17941 tgcaaagaaa aaacaaattg atagtttgtt gtctgaggct agagagtatg actttgccag
    18001 tgtttgcgtt aatccgacct gggttgaaca tgctaaaaaa ggacttgaag gcacagatgt
    18061 taaggtttgc acagtagtag gtttcccttt gggagcaaca acttcagccg tgaaagcatt
    18121 tgagacaaaa gaagctatcc aaaatggtgc agatgagatt gatatggtga tcaatgttgg
    18181 agatctcaaa tcaggtaatt tagccttggt tgagtcagat attcgcgcag tagtggaagc
    18241 aagtggtgat aagttagtga aagtcattat tgaagcttgc cttctgacag accaagaaaa
    18301 aattgttgtt tgccaattgg cccaaaaagc tggggctgac tttgtcaaaa catctactgg
    18361 cttttcaact ggtggtgcta cgatagcaga tgttacatta atgcgtgaaa cagttggatc
    18421 tgatatggga gtcaaggctg ctggtggagc tcgttcttat gcagatgctc ttgcctttgt
    18481 cgaagcaggt gcgacccgta tcggaacgtc agctggggta gctattttaa aaggagaatt
    18541 ggcagatggc gactactgag ttgattgaac tggcaattga aaccagcaaa catgcctatg
    18601 tcccctattc tcactttcct atcggagcag ttttagtagc caaagacgga aatgtttaca
    18661 cgggagtgaa tatcgaaaat gttagctatc ctttgaccaa ttgcggtgag agaacagcta
    18721 tttttaaggc tatatctgaa ggccaaagag aattttcaga attgattgtc tatggtcaaa
    18781 ctgaaaaacc gatttcacca tgtggtgctt gtcgccaagt aatggtcgaa ttttttgaac
    18841 aagatttaaa agtgacctta gtcgcaaaag ataaatcgac ggtcgagatg acggtcgggg
    18901 agttacttcc atattctttt acagacttaa attagtctga gtcgctcttt gagtggcaag
    18961 ggtctttgtg accaatcaat ccatacttgc aacatcgttg cacatcttat ttaggaggtt
    19021 cagtaatgaa caagaaacaa tggctaggtc ttggcctagt tgcagtggca gcagttggac
    19081 ttgctgcatg tggtaaccgc tcttctcgta acgcagcttc atcttctgat gtgaagacaa
    19141 aagcagcaat cgtcactgat actggtggtg ttgatgacaa atcattcaac caatcagctt
    19201 gggaaggttt gcaggcttgg ggtaaagaac acaatctttc aaaagataac ggtttcactt
    19261 acttccaatc aacaagtgaa gctgactacg ctaacaactt gcaacaagcg gctggaagtt
    19321 acaacctaat ctttggtgtt ggttttgccc ttcataatgc agttgaagaa gcagctaaag
    19381 aacacactga cttgaactat gtcttgattg atgatgtgat taaagaccaa aagaatgttg
    19441 cgagcgtaac tttcgctgat aatgagtcag gttaccttgc aggtgtggct gcagcaaaaa
    19501 caactaagac aaaacaagtt ggttttgtag gtggtatcga atctgaagtt atctctcgtt
    19561 ttgaagcagg attcaaggct ggtgttgcgt cagtagaccc atctatcaaa gtccaagttg
    19621 actacgctgg ttcatttggt gatgcagcta aaggtaaaac aattgcagcc gcacaatacg
    19681 cagccggtgc agatattgtt taccaagtag ctggtggtac aggtgcaggt gtctttgcag
    19741 aggcaaaatc tctcaacgaa agccgtcctg aaaatgaaaa agtttgggtt atcggtgttg
    19801 accgtgacca agaagcagaa ggtaaataca cttctaaaga tggcaaagaa tcaaactttg
    19861 ttcttgtatc tactttgaaa caagttggta caactgtaaa agatattgct aacaaggcag
    19921 aaaaaggaga attccctggc ggtcaagtaa tcgtttactc attgaaggat aaaggggttg
    19981 acttggcagt aacaaacctt tcagaagaag gtaaaaaagc tgtcgaagat gcaaaagcta
    20041 aaatccttga tggaagcgta aaagttcctg aaaaataatg gatggaagtc atttcttagg
    20101 aagcggccaa gggccgcttt tttaagagtt gagacaaagt cattttgtct cagtaaagct
    20161 tgctactaga caaactagca agttttattg aaataaaata agactctgaa aggaagagca
    20221 catggcacac gaaaatgtca ttgagatgcg tgatattacc aaggtgtttg gtggatttgt
    20281 tgccaacgac aaaatcaact tgcacctacg aaaaggtgaa attcatgcac ttttaggaga
    20341 aaatggggct ggtaagtcca cgctaatgaa catgttagca ggccttcttg aaccaactag
    20401 tggtgaaatc gcggtcaacg gtcaagttgt caatctcgac tccccatcta aagcagctag
    20461 cttgggaatc gggatggttc accagcactt tatgttggtt gaagccttca cagtggctga
    20521 aaacatcatt ttaggtagtg aattgactaa aaatggtgtg ctagatatcg ctggagctag
    20581 caaagaaatc aaggctcttt ctgaacgtta tggcttagct gttgaccctt ctgccaaggt
    20641 agcagatatc tcagttggag cccaacaacg tgtagaaatt ttaaaaacac tttatcgggg
    20701 ggctgatatc cttatctttg acgaaccaac ggctgttttg actccatcag aaattgatga
    20761 gttgatggct attatgaaaa atcttgtcaa agaaggaaaa tcaattatct tgattaccca
    20821 caaattggat gaaattcgcg cagtttctga ccgtgttaca gttatccgtc gtgggaaatc
    20881 aattgaaacc gttgaaattg caggggctac caatgctgat ttggcggaaa tgatggtagg
    20941 acgttctgtt tcctttaaaa cagagaagca agcctctaaa ccaaaagaag tggttttgtc
    21001 tatcaaagat ttggtggtca atgaaaaccg tggtgttcca gctgttaaaa atctgtcctt
    21061 ggatgttcgt gctggagaga ttgttggtat tgcggggatt gatggaaatg gtcagtctga
    21121 actgattcaa gccattacag gtcttcgtaa ggttgaatct ggtagcattg agctaaaagg
    21181 agattcaatt gtaggcttgc acccacgtca gattacagaa ctaagtgttg ggcacgttcc
    21241 agaagaccgt caccgtgatg gcttgatttt ggaaatgatg atatctgaaa atattgccct
    21301 tcaaacctac tataaagaac cacatagtaa aaatggaatt ttgaattatt caaatattac
    21361 ttcttatgct aaaaagctga tggaagagtt tgatgttcgc gctgccagtg aattagttcc
    21421 tgcagctgca ctctcaggag gaaatcaaca aaaagcaatt attgctcgtg aaattgatcg
    21481 agatcctgat ctccttatcg ttagccagcc aactcgtggg ttggatgtcg gtgccattga
    21541 gtatatccac aaacgcttga ttgaagagcg tgataatggc aaggctgtcc ttgttgtcag
    21601 ctttgaattg gatgagattt taaacgtctc agaccgtatt gccgttatcc acgatggtaa
    21661 gattcaaggt attgtatcac cagaaacaac caataaacaa gaacttggtg tcttgatggc
    21721 tggtggaaac ttgggaaagg agaagagtga tgtctaaaaa attacaacaa atttcggttc
    21781 ccttgatttc tgtatttcta ggaattttac tcggagccat tgtcatgtgg atcttcggtt
    21841 atgatgctat ttggggctac gaagaattgt tctatacagc ctttggcagt ctgcgtggga
    21901 ttggagaaat cttccgtgct atgggtcctc tggtcttgat tggtcttggt tttgcggttg
    21961 ccagtcgagc tggtttcttt aacgtcggac ttcctggtca ggctttggca ggttggattc
    22021 tcagtggttg gtttgcgctg tcgcatccag atatgccccg tcccttgatg attctagcaa
    22081 ccatcgtgat tgccttgatt gctggtggga ttgtcggagc gattccaggt attcttaggg
    22141 cctatctagg gacgtcagag gttattgtaa ccatcatgat gaactacatt gtcttgtatg
    22201 tagggaatgc ctttatccat gctttcccta aagacttcat gcaaagtaca gattcgacca
    22261 ttcgtgttgg ggctaatgca acctatcaga caccttggtt ggctgagttg actggtaact
    22321 cacggatgaa tattggtatt ttctttgcca tcattgccgt tgcagttatt tggttcatgc
    22381 tcaagaaaac aactcttggt tttgaaatcc gtgcagttgg tcttaatcca catgcttcag
    22441 aatatgctgg tatttctgcc aagcggacta ttatcctatc tatgattatt tcaggtgcct
    22501 tggcaggtct tggtggagct gttgaaggtt tgggaacctt ccagaacgtc tatgttcaag
    22561 gttcgtcatt agctatcgga tttaacggaa tggcggttag tttgcttgcg gccaactcac
    22621 caattggtat actctttgca gccttcctat ttggcgttct ccaagttggg gctcctggta
    22681 tgaatgcggc gcaggtacca tctgagcttg tcagcattgt aacagcgtct attatcttct
    22741 ttgtcagtgt tcattacctt atcgaacgct ttgtcaaacc gaaaaaacaa gttaaaggag
    22801 gtaagtaaag atgtctatta taaccttgct ccccctcttg gtgtcttcta tgctgattta
    22861 ctcagcacct ctcatcttta caagtatcgg tggtgttttc tctgaacgtg gtggtgtggt
    22921 aaacgttggc cttgaaggaa ttatggttat gggtgccttt tctggagttg tctttaacct
    22981 tgaatttgca gaacaatttg gagcagcaac tccatggcta tccttgcttg tagcaggatt
    23041 ggttggtagt gttttctcta tcatccacgc agcagcgacg gttcatttcc gtgcagacca
    23101 tgttgtcagc ggtacggtat tgaacttgat ggcgcctgcc ttggctgttt tcttagttaa
    23161 agttctttat aacaaaggac aaaccgacaa cctaagtcaa acttttggac gctttgattt
    23221 cccagtcttg gcaaatatcc cagtgattgg tgatatcttc ttcaagtcaa ctagtctact
    23281 tggttatctg gcgattgcct tctcattcct tgcttggttt attctcttta agactcaatt
    23341 tggtcttcgt ctccgctctg tcggtgaaca tcctcaagca gcggatacct tgggaatcaa
    23401 cgtctacaag atgagatatt tagggattat tatttcaggt tttctaggtg gaattggcgg
    23461 agcgatttat gcgcaatcaa tctcagttaa cttctcagtg acaactattg ttggacctgg
    23521 atttatcgcc cttgctgcga tgatctttgg gaaatggaat ccaatcggtg ctatgctttc
    23581 tagtctcttc tttggacttt cacaaagttt ggctgttatc ggttctcaat tgccgttcct
    23641 acaaggagtg ccagcggttt atcttcaaat tgcaccttat gttttgacaa ttcttgtctt
    23701 agcagccttc tttggaaaag cagtcgcacc aaaagcagat ggtatcaact acatcaaatc
    23761 aaaataagca tacaaaaaaa cgtcagtcca atga
//
LOCUS       DBEROU010000035        23756 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.035, whole genome shotgun sequence.
ACCESSION   DBEROU010000035 DBEROU010000000
VERSION     DBEROU010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 23756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23756
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.035"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(98..1987)
                     /locus_tag="ACL8CL_001507"
     CDS             complement(98..1987)
                     /locus_tag="ACL8CL_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HGS1330736.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKVQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWSRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKDQSKQLVTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSRFERDCLNLAFSGKKEATLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGLEKTLQVGMG
                     VLTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            2125..3690
                     /locus_tag="ACL8CL_001508"
     CDS             2125..3690
                     /locus_tag="ACL8CL_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HGS1330737.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQITGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMVQVEPGF
                     KTGEEDTAIAIGLRKDDTRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTIIGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWVLNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TTGANQMQTEDLK"
     gene            3690..4454
                     /locus_tag="ACL8CL_001509"
     CDS             3690..4454
                     /locus_tag="ACL8CL_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HGS1330738.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(4653..4868)
                     /locus_tag="ACL8CL_001510"
     CDS             complement(4653..4868)
                     /locus_tag="ACL8CL_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330739.1"
                     /translation="MAKTNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNYEKFED"
     gene            complement(5738..6988)
                     /gene="ilvA"
                     /locus_tag="ACL8CL_001511"
     CDS             complement(5738..6988)
                     /gene="ilvA"
                     /locus_tag="ACL8CL_001511"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HGS1330740.1"
                     /translation="MLSSKDIIKAHKVLNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIQTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(7038..7394)
                     /locus_tag="ACL8CL_001512"
     CDS             complement(7038..7394)
                     /locus_tag="ACL8CL_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGS1330741.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(7632..7868)
                     /locus_tag="ACL8CL_001513"
     CDS             complement(7632..7868)
                     /locus_tag="ACL8CL_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330742.1"
                     /translation="MLADQNKADWSYDENAVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSPFERAGATKSARYIAKWILRNKKY"
     gene            complement(7975..8997)
                     /gene="ilvC"
                     /locus_tag="ACL8CL_001514"
     CDS             complement(7975..8997)
                     /gene="ilvC"
                     /locus_tag="ACL8CL_001514"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HGS1330743.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVAEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(9063..9539)
                     /gene="ilvN"
                     /locus_tag="ACL8CL_001515"
     CDS             complement(9063..9539)
                     /gene="ilvN"
                     /locus_tag="ACL8CL_001515"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HGS1330744.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(9532..11232)
                     /locus_tag="ACL8CL_001516"
     CDS             complement(9532..11232)
                     /locus_tag="ACL8CL_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HGS1330745.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(11432..13099)
                     /locus_tag="ACL8CL_001517"
     CDS             complement(11432..13099)
                     /locus_tag="ACL8CL_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HGS1330746.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDAVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(13102..13467)
                     /locus_tag="ACL8CL_001518"
     CDS             complement(13102..13467)
                     /locus_tag="ACL8CL_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS1330747.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(13624..13812)
                     /gene="rpmB"
                     /locus_tag="ACL8CL_001519"
     CDS             complement(13624..13812)
                     /gene="rpmB"
                     /locus_tag="ACL8CL_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HGS1330748.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSCKVERV"
     gene            complement(13931..14365)
                     /locus_tag="ACL8CL_001520"
                     /pseudo
     CDS             complement(13931..14365)
                     /locus_tag="ACL8CL_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163337.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14841..16385)
                     /locus_tag="ACL8CL_001521"
     CDS             complement(14841..16385)
                     /locus_tag="ACL8CL_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HGS1330749.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVINAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            16598..16900
                     /gene="gatC"
                     /locus_tag="ACL8CL_001522"
     CDS             16598..16900
                     /gene="gatC"
                     /locus_tag="ACL8CL_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HGS1330750.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDHLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            16900..18366
                     /gene="gatA"
                     /locus_tag="ACL8CL_001523"
     CDS             16900..18366
                     /gene="gatA"
                     /locus_tag="ACL8CL_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HGS1330751.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLENIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            18366..19808
                     /gene="gatB"
                     /locus_tag="ACL8CL_001524"
     CDS             18366..19808
                     /gene="gatB"
                     /locus_tag="ACL8CL_001524"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HGS1330752.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDIHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKATILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LGGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMITIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            19932..20975
                     /locus_tag="ACL8CL_001525"
     CDS             19932..20975
                     /locus_tag="ACL8CL_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HGS1330753.1"
                     /translation="MATMKAARWHAAKDVRIQEIEVPEVLPHQVKVAVKFTGICGTDL
                     HEFLDGPIFIPTDEHVYSGQKAPVTLGHEFSGEIIEVGSDVTRVKVGDRVAVEPILAK
                     NNLVGDYNLDPNLNFVGLAADGGFAKYCVLDGDLVHVIPDSLSYEQAALTEPAAVAVY
                     AVRQSSLKAGDTAVVFGLGPIGLLIVEALRAAGASKIYAVELSPERQAKAEELGAIVV
                     RPEEGETAVEAIHRLTNGGVDVSYEVTGVPVVLGQALAAVHKAGECMVVSIWEREANI
                     NPNEFAIQEKTLKGIIAYRHIFPKVLELMEQGYFSAEKLVTKKIKLENIVEEGFIELT
                     QDKSQIKILVEPE"
     gene            complement(21000..21230)
                     /locus_tag="ACL8CL_001526"
     CDS             complement(21000..21230)
                     /locus_tag="ACL8CL_001526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330754.1"
                     /translation="MVNVKEYPLYFFSYSMEIKEQTRKLAASCSKHCFEVVDRTDEVS
                     NHIYGNVTLTWSEEIFEEYYISKGRSKFTPTC"
     gene            21353..21913
                     /gene="efp"
                     /locus_tag="ACL8CL_001527"
     CDS             21353..21913
                     /gene="efp"
                     /locus_tag="ACL8CL_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HGS1330755.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            21935..22324
                     /locus_tag="ACL8CL_001528"
     CDS             21935..22324
                     /locus_tag="ACL8CL_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS1330756.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            22317..22739
                     /gene="nusB"
                     /locus_tag="ACL8CL_001529"
     CDS             22317..22739
                     /gene="nusB"
                     /locus_tag="ACL8CL_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HGS1330757.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFVYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(22788..22982)
                     /locus_tag="ACL8CL_001530"
     CDS             complement(22788..22982)
                     /locus_tag="ACL8CL_001530"
                     /EC_number="3.4.26.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HGS1330758.1"
                     /translation="MILSHRDPISLLYYSLIGFFFALVYRSTDNLRLTILCHSFFNFL
                     NHAKPIWIFVYNYIYYHFFR"
     gene            complement(23037..23512)
                     /locus_tag="ACL8CL_001531"
                     /pseudo
     CDS             complement(23037..23512)
                     /locus_tag="ACL8CL_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246431.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(23530..23715)
                     /gene="briC"
                     /locus_tag="ACL8CL_001532"
     CDS             complement(23530..23715)
                     /gene="briC"
                     /locus_tag="ACL8CL_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HGS1330759.1"
                     /translation="MTGTNTFTVLSTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
BASE COUNT         7121 a         5322 c         4339 g         6974 t
ORIGIN      
        1 taagaaatag tttagataaa actattgact tttcttatag tttgtctttc ttctatcttc
       61 cattatatca tactttttca gtctatggta gctctcttta aaaggctccg atactgccgc
      121 cacctccgcc tccagagaag ccaccgccag aacttccact tccagaagat acggagtagg
      181 tacttgctgt atttgcgaca ctagcataat ggctcatttg cgcgcttgaa tgataaaaca
      241 tactgtgcca gccataagct acatagagat tgatatctgg attttcaact tgaatctgat
      301 gaaccttcat caaatgacta accttgtccg catagccaaa taaggttgca tagaccaaga
      361 gacgattcca aacaacaata ctttccaatt cagccttatc cagtcgtgca atctcacgta
      421 acatattttc aaaactggtc cagagatagt agacctcagc tcccgcttca tttagaacac
      481 catcacgatt atctagtcga agcttccaat aatagaaaac agacaaaact aaccctagaa
      541 aaccaagtat tggcaaaggg aggtaaagat agccatgaac gtctaaactg tacaagaaca
      601 aaccaaatcc gataaatagg ggcaagatag tcaagacacc catacccact tgcaaagtct
      661 tttccaaacc agttaaagga cgatagtaat ctgggagccc ccagaaggaa actcgttttc
      721 tcactccttc ttgcatctgg ttcaatacct cttcaaaaga agatttgagt tgaagccctc
      781 ttgcttgaat ccgtttttca tcagaaactt tggctctacg ataaagacta tcagatacct
      841 tgtaatccgc aaacaaattg gaaagagtgg cttctttttt gcctgaaaag gctagattta
      901 gacagtctct ctcaaatctt gacaaaccat cttctttcac caatctcaag ccaactgcat
      961 ctccttctga aataatagag acattcccac ggtctatcac atctagcaag gtagcttgaa
     1021 taagttgatc aaaggtgaat tttccagctc ccttgaccaa gggactcact tcctccaagg
     1081 aggtcgagta gactgcttct gataaaacca taggctctaa ttccattggt ggttcataga
     1141 gacgatgatt tttggcatat ttgactgaag gagtggtctt tcttctataa ataaaataga
     1201 agcagacact caataacaag gagatagaaa ggatcgaagg gaggacccaa gtaacgagtt
     1261 gtttactctg atctttttct ctaacaatcg agtcttctat cttattaaac tcttctaaac
     1321 gattcccttt caagccctga tccctagcgc tagcaaaatc ggtccgagac caataggcat
     1381 gcaactcaac tccacgctta gccggaagat tgtctaaacg gatagtataa tcaaggttac
     1441 tcttttcaat cgttccctct ctaaaaagtt tccctgtatg gaaaaagagt ttttcagccc
     1501 ccttgtctcc ccttacatga aattcaaact tttcaataga ctctgaacta tctgtcagag
     1561 gttgccaatt taattcagcg atatcatcat aaaggaaaag taaatttttt aagttccaga
     1621 cgaggtcaac ttcaactatg tcgccctcct gacctggatt atagactctc acagtataac
     1681 catccgcttc ttctgttact tcgctagtca catctgctag ttctgcaccg tttttcgcag
     1741 cctgaacctt tggatgaggg tcaatgtcaa acccgctagg catcttacca gcacgtccaa
     1801 gtcccacgat ttggccctta aagtcctcct caaactggta aactatcttc tgtctaaact
     1861 ctgccgtatt atctgcatga atatacaaat ccccttgata agagtttatc ttgaaatcaa
     1921 tggcaaaaac agagagtgga agaaggcaaa acaagcctaa caccagtaag aaaaaagttt
     1981 ttttcatcaa ctaagccccc tttttatctt tcttatcatt atagcatttt ttcgtaagta
     2041 aaggcttact tatgttgaaa aattacgcat ttttcgataa aataggagac agttattttt
     2101 taatagatca gaataggaga aatcatgaga aaaatatact tatctatttt cacaagtctc
     2161 ttgctgatgc taggacttgt caatgttgct caagccgatg aatatttacg catcgggatg
     2221 gaagcagcat atgctccctt taactggacc caggatgatg atagcaacgg agctgtcaaa
     2281 atcgatggga ccaatcagta tgccaacgga tacgatgttc aaatcgccaa gaaaatcgct
     2341 aaggacttag gtaaagaacc tttggttgtt aaaaccaagt gggaaggtct agtccctgcc
     2401 cttacttctg gtaagattga catgattatc gcaggtatga gtccaactgc agaacgcaaa
     2461 caagaaattg ccttttcgag cagttactac actagcgaac cagttctact ggtcaaaaaa
     2521 gattctgcct atgcaagtgc taaatctttg gatgacttta atggtgcgaa aatcacttct
     2581 caacaagggg tctaccttta tgacttgatt gcacaaatca caggtgctaa aaaagaaaca
     2641 gccatgggag acttcgctca aatgcgccaa gctcttgagg ctggtgtcat cgacgcctat
     2701 gtttctgaac gcccagaagc tctgactgcc gaagccgcta actctaagtt caagatggtc
     2761 caagtagaac caggtttcaa aactggggaa gaagatacag ctatcgccat tggacttcgt
     2821 aaagatgaca ctcgtattag ccaaatcaat gccagcattg aaaccatttc aaaggacgac
     2881 caagttgccc tgatggatcg tatgatcaag gaacaacctg ccgaagctac aacaactgaa
     2941 gagactagca gtagtttctt tagccaagtc gctaaaattc tttctgaaaa ctggcaacaa
     3001 ctcttgcgtg gtgctggtat cactctttta atctctatcg tcggaaccat cataggtctc
     3061 attattggac ttgccattgg tgttttccgt actgctcctc tctctgaaaa caaagccatt
     3121 tatggccttc aaaaactagt cggctgggtt ctcaatgtct acattgaaat tttccgtggt
     3181 accccaatga tcgttcaatc gatggttatc tactatggaa ctgcccaagc tttcgggatc
     3241 aaccttgacc gtacactggc tgctatcttc atcgtttcaa tcaataccgg tgcctacatg
     3301 actgaaatcg tccgtggtgg tatcctagca gttgacaagg gacaatttga agctgcgact
     3361 gctcttggta tgacccataa ccagaccatg cgtaagattg tcctacctca ggtagtccgt
     3421 aacatcctac ctgcaactgg taatgaattt gtcatcaata tcaaagatac atctgtattg
     3481 aacgttatct ctgttgtcga actttatttc tcaggaaata ccgtggcaac acaaacctat
     3541 caatacttcc agacatttac aatcatcgcc gtgatttact ttgtcctcac cttcaccgta
     3601 acacgtatcc tacgatttat cgagcgccga atggacatgg atacctatac tacaggtgct
     3661 aaccaaatgc aaacggagga tttgaaataa tgacacaagc aatccttgaa attaaacacc
     3721 tcaaaaaatc ctatggacaa aacgaagtgc taaaagacat ttcactcact gtccacaagg
     3781 gagaggtcat ctctatcatc ggaagctctg gaagcggaaa atcgaccttc ctacgctcca
     3841 ttaacctact tgaaacacca actgatggac aaatccttta tcatggacaa aacgtcctcg
     3901 aaaaaggcta tgacctcacg caataccgtg aaaagttggg gatggttttc caatccttta
     3961 acctctttga aaatctcaat gttcttgaaa acacaatcgt cgctcagaca actgtcctaa
     4021 aacgcgaacg cacagaagct gaagaaattg ccaaagaaaa cctggaaaag gtcggcatgg
     4081 gagaacgcta ctggcaagcg aaacccaaac aactctcagg tggtcaaaaa caacgtgtgg
     4141 ccattgctcg tgccctttca atgaatcctg acgccattct ctttgatgaa ccaacatcag
     4201 ctctcgatcc agaaatggtt ggagaggtcc tcaaaatcat gcaagatctg gctcaggaag
     4261 gcttgaccat gattgtcgta acccatgaaa tggaatttgc ccgtgatgtc tctcaccgtg
     4321 ttatctttat ggataagggt gtgattgctg aagaaggtaa accagaagac ctcttcacta
     4381 atcctaaaga agaccgtaca aaagaatttc ttcaacgtta tctcatactc aatgaaaatc
     4441 aaagagcaaa ctaggcagct agccgcaggt tgctcaaaac actgttttga ggttgcagat
     4501 agagctgacg tggtttgaag agattttcga agagtatcaa ataaaaagaa aaggctgcat
     4561 caaccgtgca gtctttttgc tgtcctcaaa atgaaaaggc agattctcta tctctactac
     4621 ttgaaggtca agaatccgcc gttatccaaa gattaatctt caaatttttc ataatttttt
     4681 tcatagaaat caattaggcc tagggctgtt tcaaatgaaa tattttttac ttttgcacga
     4741 ccctgcgcca gagcaatgat agacatttca cgcgcattcg tttctttaga gatacggtag
     4801 cctgtgattt tcttatcacg aacccagcca acaactgatt ctactttttc aaagtttgtc
     4861 ttagccatgt ttttctccta ttttcttctt tataatattt aattgtatac gtttttacta
     4921 cttttgtcaa taaaaaaaac atatattcaa cgaaaataat tagttcagaa ttttcttact
     4981 tcctttttaa atccgataat atataggttt tgctagcctc tacaccataa atatttgcta
     5041 ataaaaatac tgtattatta ccctcttaag gtgcgaaatt attcaagttc ttaaaaatgc
     5101 ttctctattt ccgtaagata gcttatttct tcgatttaag aactgaagat gatagttcta
     5161 acctttagaa atccaagcct tcactagctc aaaaactcct atctttcttc ctcaataaat
     5221 catctgagag ataggtattc cttatatcct tcaaattcat cactcctttg gatattgagg
     5281 tgctagccta gaagcagaca cccgattatc taaaccatcc ttaataaaga tgacaagaca
     5341 gataaactca gttaattact tatatatcct tttaatatgg tatatttgtc aacaaaaaga
     5401 aatcaaatca taccttatac tcaatgaaaa tcaaagagca aactaggaag ctagccgcaa
     5461 gttgctcaaa acactgtttt gaggttgcag atagagctaa cgtggtttga agagattttc
     5521 gaagagtatt aaagacagtg tgactatgct tgtcaataga taggaaatca aaatgaagga
     5581 tggaaatctg agccaaacat cataagtgct ataatgaaca gaagtaagaa ttgcacgata
     5641 agttttctaa aaccatagga atcgtcccct ttcttcatct tcattatagc acatatacat
     5701 cagttgtaca aataatatga tattttttta ttagtcctca gacaagcata ttataaagcg
     5761 tttcattacc atttaagtta atataagctg gatcaaaacc ttccattcta cggatcaaac
     5821 ctgcataatc atgcttatct gccaaagcga tcccaatcaa tactgggcct gttcccttgc
     5881 tagctcgttt gatatactca aaacgtgtga tatcatcatt tggccccagg atatcattta
     5941 caaactcacg caaagctcct ggacgctgtg gaaaattgac cacaaagtaa tgtttgatac
     6001 catcataaat caaggcacgc tcttccattt ctggcatacg gttgatatca ttatttcctc
     6061 cagaaatgat acaacaaatg gttttcccct taatatattc agctaaaacc tctaaagagg
     6121 cgatactagc cgctccagca ggctctgcga ctatcccttg cttagagtaa aggtcaatca
     6181 aggtttcaga aatcaatccc tcatcgacac ctaccaaagt ttgaatatgt tgacgagttg
     6241 cttcataggt caactgacct accttttgca cagcaatccc atccgcaaat ttatcaattt
     6301 ccttgagttt gactggacct ccagcctcaa aggcagcttt catggaacgc gctccattcg
     6361 cctctactcc gataacctca atctctggac ttgtttcctt gatataggta gaaaccccgg
     6421 caatgagacc gccaccacca acaggaacca agacagcatc aaaatcaatc gattcttttc
     6481 gagcttcttc taaaatctca taagcaactg ttccttgacc tgcttgaaca tgagcatcat
     6541 caaaaggatc aataaaggta cgattttcag agactgtaaa ttcttgagct gctttggctg
     6601 aggcatcaaa ggtatctcca actagtttaa tagttacaaa atccccacca aaaaagcgaa
     6661 cctgaccaat cttttgttgt ggcgtagtaa taggcataaa gatagtagca ggaattttca
     6721 tttcattaca agtataggct actccctgcg catgatttcc cgcagaagcg cagactaccc
     6781 cacgttcacg ttcttccttg ctgagctggg aaatggcata ataggcacca cgaattttaa
     6841 aggagcgaac acgctgggca ttttcttttt tcaaataaat cttagcacca tacttctccg
     6901 ataaataatg atcgtaatcc agtggagtat tcacaaccac accgttcaag accttgtgag
     6961 ccttgattat atcttttgaa cttaacatat aaaacctctt tttctataaa aaacagtcac
     7021 taatgactgt tttttagtta ttcatctccg aaaacttcag taacactttc atggtattta
     7081 tgagccatac gaatcgaaaa ctctttagga agttcataac gatttgtgga aatccaatca
     7141 tataaccatt ctaccaaact ttcttcacta tcattacgat ggaagataaa atagtttaaa
     7201 atttctttat attctacatc attcaaatcc aacgtgtcta caaattgact acgaggaaca
     7261 aaattagaat ctaattttct tagttgatct gtcaattctc ccaattgtaa atccaactga
     7321 aaaaatagtt tttgcagttg cctatcagtt tcatcacatc ctgtcttgat agaaaacaaa
     7381 ttggtaatat tcatatgatt tcaaccccta aataagtaca tctaatatcc cacgaatagc
     7441 tgaagctaaa gttttcgcag tccagtcatt taaacccgat tctttcaatc cagatgcaat
     7501 aatttgctct atttgatata aaccgccatc tatcgtctca tttattctgc gaagaggacc
     7561 ttcaatatag gataaagctt ctccgaatct aacagctgat ttaggtccta atgatttacc
     7621 aactatattt gttaatattt tttatttctc aaaatccatt tcgctatata acgagcagat
     7681 ttagtcgcac cagctctctc aaacggagaa tgaatttcaa gaccttcttc tctagctatt
     7741 tgtgccaatt tgaagaattg ttcaaaggtc atatgcaacc taccatcttc aaaattagca
     7801 tcatcataaa tgttaattac agcattttca tcatacgacc aatctgcttt attttggtct
     7861 gctagcacta ttaccgacgg tgtaattaca ttatacaact agaaccaata ataacgatgc
     7921 aatttttctc ataatctttt tcataaaatc atctccagcg ctatatattt ctaattagtt
     7981 atagattttg aatgcatcat cgtcgttttt accaacgaat ggcattgctt tacgcaattc
     8041 tgcaccaact ttttcaattt caaggttagc tgcttgttca cggtaagcag tcaattttgg
     8101 acgtccagct ttatagtcat ttacaaagtc atttgcaaat ttaccatttt ggatgtctgc
     8161 caagacagcc ttcatatttt ctttaacttg ttcagtgatt acacgtggac ctgatacata
     8221 gtcaccgtat tcagcagtgt ttgaaataga ttgacgcatt ttcttgaatc caccttcgta
     8281 gatcaagtca acgatcaatt tcatttcgtg aagaacttca aagtaagcca attctggagc
     8341 gtaacctgct tctgtcaaga cttcgaaacc tgcttcgata agggcagtca aaccaccaca
     8401 aagtacagct tgttcaccaa acaaatcttc ttcagtttct tctttgtaag ttgtttcaag
     8461 aagacctaca cgagccgctc caacaccttt acaccagtcc atagcaatgt ttttagcatt
     8521 tcctgttgca tcttggtata ctgcataaag agctggaaca ccaaatcctt cttcgtaagt
     8581 acgacgtacc aagtgtcctg gtcctttagg agcacacatg aagacatcta catccgcagg
     8641 aactttgata aattcaaagt ggatgttgaa accatgggca aatccaactg cgtttccagc
     8701 ttccaagttt ggagcgattt ctgcttcgta caattcttgt tgaatttcgt ctggcgccaa
     8761 gatcataata acatcagcca acttagtagc ttctgctact gtgtaagtat caaatccatc
     8821 ttcttttgct ttatcaaaag atttacctgg acgtacaccg ataataacgt cacgacctga
     8881 atcacgcaag ttttgagcat gcgcatgccc ttgtgaacca taaccgataa cggcgatttt
     8941 tttaccgtca agtgctgcta ctttaacatc tttttcatat tccatttgaa ctgtcatagc
     9001 ttttctctct tttctattat ttattgcctt ttaggctggt ttaataaatt taagttggat
     9061 ttttaatcgc gggtaaatcc agttgcaccc gttcgagcaa tattgcgaat accgtatggg
     9121 cgaatgactc gcaatagggc ttcgctcttt tctgcatttc ccgtcatctg aatggtaatc
     9181 gagcttggcg ctacgtctac tactgttgca cggaaaggtt gaataatcgc taaaatctca
     9241 gctctcttct cagctggcgc tgacatctta accaaaatca cctcgcgctc caaatgaggc
     9301 ttgtctgtaa tatctcgaat gcgaatcaca tcaatctgac gattgagctg tttgatgatt
     9361 tgctccactt catcatgaga agcaacatca ataataatag tgatacgcga tacattcgga
     9421 tcttctgttg ctccaacaga gatgctttca atattaacct gacgacgaga taggacacct
     9481 gtaaagcgat tgaggactcc tgaacgattt tgtagttttg ctgttaacat tctacgcatg
     9541 gaactgcacc cccaacatct catgattact cttaccagcc ggtaccattg gtaacacctg
     9601 ttccttacga gaaatatcta cctcaattag cataggaaca tcctcagtga tgacttcaag
     9661 gtcttgagcc aaggtctcag gattgtcaaa cttatagttt ttaataccat aagcctgcgc
     9721 catcaattgg aaatcaggaa gggtatcaaa gaccgactct gatgttctgc cttcatagaa
     9781 ggattcctgc cactggcgaa ccattccaag tgaatgattg ttcagcataa ccaccttgat
     9841 tggcaccttg taaatattca aaatagccaa ctcctggttg gtcatttgga aaccaccatc
     9901 cccaacaaac aagactactt ccttatctgg gttagcaatt ttagcaccga ttgctgctgg
     9961 aattccaaag cccattgttc ccaaaccacc tgaagtcact aactgacgtt cattttggta
    10021 gggataatac tgagctgtcc acatttggtg ttgaccaacg tctgttacca caatggcatc
    10081 tccattcgtc aattcaccaa ttcgttcaat aactgcttgc ggttgaacca cacgctcttt
    10141 cttgtcatac gaacgaacac gattcttgtc tttagtgact ttctcaatcc acttttcagt
    10201 attgttgtga actgttggtt ctgctagcaa catttgcaag gccttcttag catctccaac
    10261 tacaggaatg tctgcactga taatcttgcc aatctcagct gggtcaatat caatgtgggc
    10321 aaccttagca ttcttagcga aagtcttagg attccccgtc aaacggtcat cgaaacgaga
    10381 accaatacta atcataaagt ccgcttccgt catggcaata tttgctgcga atgacccgtg
    10441 catgcctccc attccaagaa agagtgggtg actcgttgca atcgttcctt gtcccaaaag
    10501 actggttacc actggaattt gatagcgttc tgcaaattca tttagttccg tagcagcctc
    10561 agcataacta attccaccac cagctaacaa gactggcttt ttagccttgg acaattgctt
    10621 caagattttc ttgatttgca tatcattcgg ctcaagagtc ggctgataac ttggtaaatt
    10681 cacttctggt gaataaatga agtctgtttc taaagcagat atgtcttttg gtaggtcaat
    10741 tacaactggc cctggacggc ctgtagttgc gatatggaca gcttccgtaa tgatacgcgg
    10801 aatatcagct gtctcacgaa cttggtaatt gtacttagtg attggcatgg taattcctac
    10861 gatgtctgcc tcctgaaagg catccttccc aatccctgct cgcgccacct gacctgtaaa
    10921 gaccaaaagg ggaacgctat cgctcatagc atccgcaatc cctgtaatgg catttgttgc
    10981 tcctggtcca ctagtgacga cggcaacacc caactttcca gttgatttgg catagccttc
    11041 agcttcatgc aaacaacctt gctcatggcg ccctagaatg tggcgaatgc ctttaaaatt
    11101 atatatcgca tcataaaaag gcaaaaccgc accaccagga taaccaaaga tggtatcaac
    11161 tcctaaatca cgaagtgttt ccaaaactag gtccgacccc gtcttaggag attctaaact
    11221 gattttctcc attattcccc tttctttcct cttaaaaata acttgttact atcataccat
    11281 tttttcaaaa tttttcaaga taaaagaaga aattttctga attttctatt ttaacgttta
    11341 tttatgaatg ttatttttgt ttttattaaa aagataccga tttcatttac taaaaagaaa
    11401 aaagaactga tttctcagtc cttcattaat cttattccac actaaatagg tatgggtaaa
    11461 caggttgttg accttggtga atctcgactt caacgtcttc gaattcttct acgatttctt
    11521 gagcgatttc attggcaagt tcttcgcttc cgtcttcacc tacatagaag gttacgattt
    11581 cactgtcttc atccaacata tgtttcaagg tttcagtcaa tgtttggtgc atatcagggt
    11641 ttgacacaag aatttttcca tccaccatac ctaaattatc gttttcatgg atttctaagc
    11701 catcgatcgt tgtatcacgc acggctgttg tgacgcttcc gctaacgaca tcgctaagag
    11761 cagctgtcat acgctcttgg ttttcttcaa tggacttgct tggatcaaag gcaagaagac
    11821 ttgtcatacc ttgaggaaga gtgcgagcct ctaccactac cgctggttgc tccaaaactt
    11881 ctgccgcaga ttgagctgcc atgaagatgt tcttgttgtt tggcaagaag atgatgttac
    11941 gggcattaac ctgttcaaca gccttgataa agtcttctgt tgaagggttc atggtttgac
    12001 cgccttcgat aacataatcc acgccttgag aacagaagat atctgctaga cctttaccag
    12061 ccaccacagc aatcaaagca tactcttttt cttcagccga cttgataact tgagtagctt
    12121 ctttctcaac ctgtgcttcg tgttggttac gcatattgtc aacttttacc ttgaccaagc
    12181 taccatattt gagaccttct tgcataacaa gtcctggatc ttctgtatga acatggactt
    12241 tgacaatttc atcatcgtta acaacgagga gagaatctcc aagctcatcc aagtagttac
    12301 ggaattcatc gtagtcaaaa tctttagcat aggttggacc ttgcttaaga gctaccatga
    12361 tttcagtaca gtaaccaaac gtgatgtcct cagtcgctac gtgaccagct acagacttat
    12421 gatgctctac attgatcatc tcactcatgt tggcaggagt cgctacaaag tcctcagatg
    12481 caatatattc gccagtaagg gctgaaagga aaccttcgta gatgaagacc aatccttgac
    12541 cacctgagtc cacaacgcca acttctttca atactggaag catgtctggt gttttagcta
    12601 gagctgtttt agcaccttcc aaggctgcgc gcatgacttc aacagcgtca tctgtttgct
    12661 cagctttttt cttagcaccg atagcagctc cacgagaaac tgttaaaatc gttccttcaa
    12721 caggtttcat cactgcctta taggcaactt ccacacctga ttggaaggcc agagccaagt
    12781 cttgacctgt taactcgtct ttatccttga tagcttggga aaatccacgg aaaagctgag
    12841 acgtaatcac tcctgagttc ccacgcgcac ccatcaaaag ccctttggca agaatgctcg
    12901 ctacttctcc aactgtagaa gctggcttgt ctgcaacttc tttagcacca ttttcaatgg
    12961 tcattcccat atttgtccca gtatctccat ctggaactgg aaagacgttt aatgaattga
    13021 catattcagc ttgcttattc aagcgagttg atgcagcctg caccatttct tgaaataagc
    13081 tagtagtaat ttttgacacg attattctcc tacaactttg atattttgaa tgtagacatt
    13141 tacagtctga gcagtaattc caagctggtt ttccaagcta aaggcaacac gctcttgaat
    13201 gttttttgac acttcactaa tctttgttcc gtagcttaac acggtatata catcaactgc
    13261 aatactgcca tcttcggctg cctttacgac gacaccttta gaataatttt ccttacctag
    13321 cagggcttgg aaattatctt tgagggcatt tttactagcc ataccgacca caccaaaaat
    13381 ctcagttgct gctccaccta cgacggttgc aatcacttca tctgttagtt cgatttgacc
    13441 atcttttgta ttaattttta cagtcatcct ttttacctca actagttgat actctatttt
    13501 atcatatttc agcccaagtg taaaagcaga atgatgtatc agcggatatt tactctattt
    13561 ttcaaacgat gttataccca caataaaaga aaaaagaccc taaggtctcc tttgctttta
    13621 ttattaaacg cgttcaactt tacatgattt caaagcacga gctgaagccc aaactttttt
    13681 aggtttacca tcgataagaa cagtaacttt ttgaaggttt ggttttacgg cacgttttgt
    13741 ttggttcatc gcgtgtgaac ggttgtttcc tgatacagtc ttacgacctg taaagtaaca
    13801 tactttagcc attgtgtttt cctcctatta gatctaatat agcggatgtg ctagcaccac
    13861 ataccgtact atgttatcac actttcttgt tttttgcaag ggaattggaa gattttttat
    13921 ttgtgtctta aatcaggtct tgcgtgacat tttctgctct ccacatgcca ttgttgatta
    13981 acagaacacc agaattaaaa ttatgtgtat aaaaatcatc tctaactgca gctaagggta
    14041 tagccgtcaa gtccaaatcc cacagctcat ctatcgattt tcttacaaca atatctaaat
    14101 ccaaatacag tacacgagac tcgcttacat actttggaat aaaatatcta aaaaagccgc
    14161 atatgaaagt ccctcaaagg ggagacgata acctttcaga atactactgt caatctaaac
    14221 attcacaatc tcactattca aagtctctag tcttttttcc atcaattgga accattctcg
    14281 cggaaggtca tcattaaaaa cataaaactt aagattataa tagatgaaca caaagagatt
    14341 ttattgttgt ttcaacttta tccatataag cattatctgc acctaagaca atcgcttttt
    14401 tctcttcttt cactttttat ctcatttctt tttattccca tcatatgttt ctacgtaacc
    14461 attattttcg cctattcgtt cgtaaaacca taccagtgga gattttagat gaagtcccat
    14521 tacggtttac aatttttaca ttacgacacg gagttttaca aatcgatttc atttgccaaa
    14581 cgtagttagt aaggcagtta gctaggctac ctactttctg ctacgacgga aaaatccaca
    14641 aatcaatacc attcgtggat ttttcctagt gaagcgttta ggtaagccgc catagcggtt
    14701 accgattatt agattacgac acggagtttg gcaaatcgat tctatttgcc aaacgtagtt
    14761 agtgaggcag ttagctagtt cgccaaatag cgactagcgt ccaacaattt ggaactttag
    14821 ttccaattgt tggtactgag tcacatcttc tcctctaact ctacgtctgg atacttgtcc
    14881 gcaaaccagc ggagggcaaa gtcattttca aagagaaaga ctggttggtc aaaacggtct
    14941 ttggctaaga tattgcgact tgacgacatc cgttcatcca agtcctcagg cttgatccaa
    15001 cgaacggtct ttttacccat tgggctcatg accacttccg cattgtattc gccttccata
    15061 cggtgtttaa agacttcaaa ctggagttga ccaacagctc ccagcatgta ctcacctgtt
    15121 tggtaattct tataaagctg aacggctcct tcttgcacca attgctcaat ccccttgtgg
    15181 aaggattttt gcttcataac attcttagca gaaactttca tgaaaatttc aggagtaaag
    15241 gttggcagtg gttcaaattc aaacttgttt tttccaaccg tcaaggtatc cccaacctga
    15301 taagtaccgg tatcgtaaac cccgataata tcacctgcca cggcattgat cacattctca
    15361 cgactttccg ccataaactg ggtaacatta gatagtttag cccccttacc agtacgaggg
    15421 agattgacac tcatgccgcg ctcaaattcg cctgatacga tacggacaaa ggcaatacgg
    15481 tcacggtgac gaggatccat gttggcttgg attttaaaga caaagcctga gaaatccttg
    15541 tcataaggat ccacaatttc accgtctgtt ttcttgtgac catgtggttc tggagcaaac
    15601 ttgaggaaga tttcaaggaa ggtctgcaca ccaaagtttg tcagggctga accgaaaaag
    15661 acaggcgtca attctccagc cagaatagct tcctctgaaa actcattccc agcttcattt
    15721 aaaagctcaa tgtcatcctt gacttgctcg tagaaaggat tgctaccaaa gagtttgtcc
    15781 ccgtcttcta gactggcaaa acgctcatcc cctttgtaaa gctctaaacg ttggttatag
    15841 aggtcataca agccctcaaa ggctttcccc atcccgatag gccagttcat agggtagcta
    15901 gcaatgccca agatttcttc caattcttgc aagagatcca aaggctcacg accgtcacgg
    15961 tccagcttgt tcataaaggt aaaaactgga atgccacgat gtttcacaac ctcaaacaat
    16021 ttcttggttt gagcctcgat ccccttggca gagtccacga ccatgaccgc agcatccacc
    16081 gccatcaagg tacgataggt atcttctgag aagtcctcgt gccctggcgt gtctaagata
    16141 ttcacgcgct tgccgtcgta gtcaaattgc ataacagatg aagtaacaga aatcccacgt
    16201 tgcttctcga tatccatcca gtcagattta gcaaaagtcc ctgttttctt cccttttacc
    16261 gtaccagcct cacgaatctc acccccaaag tagagtaact gctcagtgat ggttgttttc
    16321 cccgcgtccg ggtgggagat aatggcaaag gtacgacgtt tcttaatttc ttcttgaata
    16381 ttcataagtt ctctttcttt gattctctat ttttcttgtt tcaatagctg agaatgattt
    16441 ttacattgga ttttaccatt cctttcaaca ctccattata tcggatttta gcattttttt
    16501 caatttctat ttcttttcac ttccccctcc cttatttata ggaaaatatg gtaaaataga
    16561 acagactaaa aatcatcatt tcacgaaagg atgcaagatg aaaattacgc aagaagaggt
    16621 aacacacgtt gccaatcttt caaaattaag attctctgaa gaagaaactg ctgcctttgc
    16681 gaccaccttg tctaagattg ttgacatggt tgaattgctg ggcgaagttg acacaactgg
    16741 tgtcgcacct actacgacca tggctgaccg caagactgta ctccgccctg atgtggccga
    16801 agaaggaaca gaccgtgatc acttgtttaa aaacgtacct gaaaaagaca actactatat
    16861 caaggtgcca gctatcctag acgatggagg agatgcctaa tgacttttaa caataaaact
    16921 attgaagagt tgcacaatct ccttgtctct aaggaaattt ctgcaacaga attgacccaa
    16981 gcaacacttg aaaatatcaa gtctcgtgag gaagccctca attcatttgt caccatcgct
    17041 gaggagcaag ctcttgttca agctaaagcc attgatgaag ctggaattga tgctgacaat
    17101 gtcctttcag gaattccact tgctgttaag gataacatct ctacagacgg tattctcaca
    17161 actgctgcct caaaaatgct ctacaactat gagccaatct ttgatgcgac agctgttgcc
    17221 aatgcaaaaa ccaagggcat gattgtcgtt ggaaagacca acatggacga atttgctatg
    17281 ggtggttcag gtgaaacttc acactacgga gcaactaaaa acgcttggga ccacagcaag
    17341 gttcctggtg ggtcatcaag tggttctgcc gcagctgtag cctcaggaca agttcgcttg
    17401 tcacttggtt ctgatactgg tggttccatc cgccaacctg ctgccttcaa cggaatcgtt
    17461 ggtctcaaac caacctacgg aacagtttca cgtttcggtc tcattgcctt tggtagctca
    17521 ttagaccaga ttggaccttt tgctcctact gttaaggaaa atgccctctt gctcaacgct
    17581 attgccagcg aagatgctaa agactctact tctgctcctg tccgcatcgc cgactttact
    17641 tcaaaaatcg gccaagacat caagggtatg aaaatcgctt tgcctaagga atacctcggt
    17701 gaaggaatta acccagaggt taaggaaacc attctaaatg ccgctaaaca ctttgaaaaa
    17761 ttgggtgcta ttgtcgaaga agtcagcctt cctcactcta aatacggagt tgccgtatac
    17821 tacatcatcg cttcatcaga agcttcatca aacttgcaac gcttcgacgg tatccgttac
    17881 ggctatcgcg cagaagatgc aaccaacctt gatgaaatct atgtaaacag ccgaagccaa
    17941 ggttttggtg aagaggtaaa acgtcgtatc atgctgggta ctttcagtct ttcatcaggt
    18001 tactatgatg cctactacaa aaaggctggt caagtccgta ccctcatcat tcaagatttc
    18061 gaaaaagtct tcgcggatta cgatttgatt ttgggtccaa ctgctccaag tgttgcctat
    18121 gacttggact cactcaacca tgacccagtt gccatgtact tggctgacct cttgaccata
    18181 cctgtaaact tagcaggtct ccctggaatt tcgattcctg ctggattctc tcaaggtcta
    18241 cctgtcggtc tgcaattgat tggtcccaag tactctgagg aaaccattta ccaagctgct
    18301 gctgcttttg aagcaacaac agactaccac aaacaacaac ccgtgatttt tggaggtgac
    18361 aactaatgaa ctttgaaaca gtcatcggac ttgaagtcca cgtagagctc aacaccaatt
    18421 caaaaatctt ctcacctact tctgcccact ttggaaatga ccaaaatgcc aacactaacg
    18481 tgattgactg gtctttccca ggagttctac cagttctcaa taaaggggtt gttgatgccg
    18541 gtatcaaggc tgctcttgcc ctcaacatgg acatccacaa aaagatgcac tttgaccgca
    18601 agaactactt ctatcctgat aaccccaaag cctaccaaat ttctcagttt gatgagccaa
    18661 tcggttataa cggctggatt gaagtcgagc tagaagacgg tacgaccaag aaaatcggta
    18721 tcgaacgtgc ccacctagag gaagacgctg gtaaaaacac ccatggtaca gatggctact
    18781 cttatgttga cctcaaccgc caaggggttc ccttgattga gattgtatct gaggcagata
    18841 tgcgttctcc tgaagaagcc tatgcttatc tgacagccct caaggaagtt atccagtacg
    18901 ctggcatttc tgacgttaag atggaggaag gttcgatgcg tgtggatgcc aacatctccc
    18961 ttcgtcctta tggtcaagag aaattcggta ccaagactga attgaagaac ctcaactcct
    19021 tctcaaacgt tcgcaagggg cttgaatacg aagtccaacg ccaggctgaa attcttcgct
    19081 caggtggtca aatccgccaa gaaacacgcc gttacgatga agcgaataaa gcaaccatcc
    19141 tcatgcgtgt caaggaaggg gctgctgact accgctactt cccagaacca gacctacccc
    19201 tctttgaaat ttctgacgag tggattgagg aaatgcggac tgagttgcca gagtttccaa
    19261 aagaacgtcg tgcgcgttat gtatctgacc ttggtttatc agactacgat gctagtcagt
    19321 tgactgctaa taaagtcact tctgacttct ttgaaaaagc tgttgcccta ggtggtgatg
    19381 ccaaacaagt ctctaactgg ctccaagggg aagtcgctca gttcttgaat gctgaaggta
    19441 aaacactgga acaaatcgaa ttgacaccag aaaacttggt tgaaatgatt accatcatcg
    19501 aagacggtac tatttcatct aagattgcca agaaagtctt tgtccatcta gctaaaaatg
    19561 gcggtggcgc gcgtgaatac gtggaaaaag caggtatggt tcaaatttca gatccagcta
    19621 tcttgatccc aatcatccac caagtctttg ccgataacga agctgctgtt gccgacttca
    19681 agtcaggcaa acgtaatgca gacaaggcct tcacaggatt ccttatgaag gcaaccaaag
    19741 gccaagccaa cccacaagtt gcccttaaac tacttgcaca ggaattggcg aagttgaaag
    19801 aaaactagac agaacaaaac cagccctaag gttggttttt tcttgacaaa tagaaagaaa
    19861 acgtttgcaa tattgcgtgt aaagtttcca aacaagaaaa ctaaaaatcg caacaataag
    19921 gagttattta tatggctaca atgaaggcag ctcgctggca tgcagcgaaa gatgttcgta
    19981 ttcaagaaat agaagttcct gaagtacttc cgcaccaagt aaaagttgca gttaagttca
    20041 caggaatttg tggtactgac ctccatgaat ttttagatgg acctatcttt atcccaacag
    20101 atgagcacgt ctattctggt caaaaagcac cggtaacact gggacatgaa ttttctggtg
    20161 aaatcataga agtcggaagc gatgttaccc gtgttaaagt tggtgatcgt gttgctgtgg
    20221 aaccaattct agctaaaaat aacttagttg gtgattataa tttggatcca aaccttaatt
    20281 ttgtcggttt ggctgcagac ggtggatttg ccaaatattg tgttttagac ggtgacttag
    20341 tacatgtcat tccagatagt ttgagctatg agcaagctgc tcttactgaa cccgctgctg
    20401 ttgcagtcta tgctgttcgt caatcttctt tgaaggctgg cgatacagct gttgtctttg
    20461 gtttaggccc gattggtcta ttaattgtag aagctcttcg tgcagctggt gcttctaaga
    20521 tttatgctgt tgaactatca cctgaacgtc aagcaaaagc tgaagagtta ggagctatcg
    20581 ttgtccgtcc ggaagaaggt gaaacagctg ttgaagctat tcatcgttta acaaacggtg
    20641 gtgtagatgt ttcttatgaa gtaacgggtg tgccagtagt tttaggacaa gccttagctg
    20701 ccgtacataa agcaggtgag tgcatggtgg tctctatctg ggaacgagaa gcaaatatta
    20761 atccgaatga atttgcaatc caagaaaaga cactaaaagg aatcattgct tatcgtcata
    20821 tcttccctaa agtattagaa ttgatggaac aaggctactt ctctgctgaa aaattagtta
    20881 ctaagaaaat taaattggaa aatatcgtcg aagaaggctt tatcgaatta actcaagata
    20941 agtcacaaat taaaattttg gtagaaccag aataaaaaat caatcttttt aagacaatac
    21001 taacaggtcg gagtaaactt acttcggcct tttgaaatat aatactcttc gaaaatctct
    21061 tcagaccacg tcagcgtcac attaccgtat atatggttac tgacttcgtc agttctatcc
    21121 acaacctcaa aacagtgttt tgagcagctt gcggctagct tcctagtttg ctctttgatt
    21181 tccattgagt atgagaagaa atatagtgga tactctttaa cattaaccac tccacctgac
    21241 tagccaatat tctttttcgc tcctaccaac tcccaataac tattttggct ttatttccag
    21301 agtattttat ggtaaaatga agagtaataa tatttattaa agaggtaaaa acatgattga
    21361 agcaagtaaa ttaaaagctg gtatgacctt tgaaacagct gacggcaaat tgattcgcgt
    21421 tttggaagct agtcaccaca aaccaggtaa aggaaacacg atcatgcgta tgaaattgcg
    21481 tgatgtccgt actggttcta catttgacac aagctaccgt ccagaggaaa aatttgaaca
    21541 agctattatc gagactgtcc cagctcaata cttgtacaaa atggatgaca cagcatactt
    21601 catgaataca gaaacttacg accaatacga aatccctgta gtcaatgttg aaaacgaatt
    21661 actttacatc cttgaaaact ctgatgtgaa aatccaattc tacggaactg aagtgatcgg
    21721 tgtcaccgtt cctactactg ttgagttgac agttgctgaa actcaaccat ctatcaaagg
    21781 tgctactgtt acaggttctg gtaaaccagc aacgatggaa actggacttg tcgtaaacgt
    21841 tccagacttc atcgaagcag gacaaaaact cgttatcaac actgcagaag gaacttacgt
    21901 ttctcgtgcc taatctctag aaagaggtca ttctatggga attgaagaac aacttggcga
    21961 aatcgttatc gccccacgtg tacttgaaaa aatcattgct atcgctactg caaaggtaga
    22021 gggtgttcac tctttttcaa acagatcagt gtctgatacc ctttcaaaac tttcactcgg
    22081 ccgtggcatt tatcttaaaa acgtggacga agaactcaca gcagatatct atctctacct
    22141 tgagtacgga gtaaaagttc ctaaggtagc ggttgctatc cagaaagctg tcaaagatgc
    22201 cgtccgtaat atggctgatg tagaactcgc tgctatcaat attcacgttg caggtatcgt
    22261 cccagataaa acaccaaaac cagaattgaa agatctattt gacgaggact tcctcaatga
    22321 ctagtccact attagaatct agacgccaac tccgtaaatg cgcttttcaa gctctcatga
    22381 gccttgagtt cggtacggat gtcgaaactg cttgtcgttt cgtctatact catgatcgtg
    22441 aagatacgga tgtacaactt ccagcctttt tgatagacct cgtttctggt gttcaagcta
    22501 aaaaggaaga actagataag caaatcactc agcatttaaa agcaggttgg accattgaac
    22561 gcttaacgct cgtggagaga aacctccttc gcttgggagt ctttgaaatc acttcatttg
    22621 acactcctca gctggttgct gttaatgaag ctatcgagct tgcaaaggac ttctccgatc
    22681 aaaaatctgc ccgttttatc aatggactgc tcagccagtt tgtaacagaa gaacaataaa
    22741 aagaaaaagt gtttgatatc tatcaaacac tttttctttg cctcccacta tctaaaaaaa
    22801 tgataataga tataattgta aacaaaaatc cagataggtt ttgcatgatt gagaaagtta
    22861 aaaaaactat ggcagagaat cgttaatctc agattgtcgg tagaacgata aacaagggca
    22921 aaaaagaaac caatcagact ataatataat aaactaattg gatctctgtg agatagtatc
    22981 aaatgactaa gcccaaagat aaggcctgat aagatgacat ctaaataata cttggactag
    23041 ggaaagaagg tattcataaa ataccctcta tcaagagtct cctcaaaaac aggaccgatg
    23101 attacaggca ggacaaaaga taagatagtc gataaaaagg ttggttgtcc atttgaaaga
    23161 accacggtaa aatactcatc atgaatattc ctatgattaa tcaaatgagc atagcgtgcc
    23221 caaaaattac cgagaatctg ataaaccaca taagttgcaa ataaggagaa gacaaatgac
    23281 cagttccagc tctttttctc aaagataaag agcatctttt tcttttttaa cctccaaatt
    23341 aatagaaaag gaaacttccc actaatccca ctgttaaaat aagagaatag acatcagctc
    23401 ctaaccctaa aatgatcgtc acatacaatc caattgtttg tggtaaatag gtagatagta
    23461 aaataataag caaaaatatt ccaaattgtc ttagtttctt tgtgtttctc atcgtacttt
    23521 tttgaaagat taccctgttc ggaagccgta cttccaagca tctatataag aattaagtgc
    23581 cccttgcctc atataaggag caaattctct ataatataac catctactat atccatcttc
    23641 ccaaacagca agaccacctg aagtttgctc caagtcctca gttgaaagaa ctgtaaatgt
    23701 atttgtacct gtcattgcaa gtaccttctt aaaatatagt gatttgaaat aagatg
//
LOCUS       DBEROU010000036        22562 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.036, whole genome shotgun sequence.
ACCESSION   DBEROU010000036 DBEROU010000000
VERSION     DBEROU010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22562
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.036"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            55..279
                     /locus_tag="ACL8CL_001533"
     CDS             55..279
                     /locus_tag="ACL8CL_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000269692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HGS1330760.1"
                     /translation="MIAGGLIGLLAGKITKKSSSMGIIANVFAGLVGASAGQSLLGSW
                     GSSIAGMALLPSIVGAAIVITVVSFFTGRK"
     gene            363..929
                     /gene="amaP"
                     /locus_tag="ACL8CL_001534"
     CDS             363..929
                     /gene="amaP"
                     /locus_tag="ACL8CL_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HGS1330761.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            941..1111
                     /locus_tag="ACL8CL_001535"
     CDS             941..1111
                     /locus_tag="ACL8CL_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HGS1330762.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1150..1758
                     /locus_tag="ACL8CL_001536"
     CDS             1150..1758
                     /locus_tag="ACL8CL_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HGS1330763.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1789..1992
                     /locus_tag="ACL8CL_001537"
     CDS             1789..1992
                     /locus_tag="ACL8CL_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HGS1330764.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2087..2326)
                     /locus_tag="ACL8CL_001538"
     CDS             complement(2087..2326)
                     /locus_tag="ACL8CL_001538"
                     /inference="COORDINATES: protein motif:HMM:NF025126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1330765.1"
                     /translation="MNERYQCLKTKEYQALLSSKGRQIFAKRKIDMKSVFGQIKVCLG
                     YKRCHLRGKRQVRIDMGFILMANNLLKYNKRKRQN"
     gene            2605..2850
                     /locus_tag="ACL8CL_001539"
     CDS             2605..2850
                     /locus_tag="ACL8CL_001539"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGS1330766.1"
                     /translation="MAESIQGWLAQFLVNLFKSITFDCGKEFSKWKDISNHHDSESFF
                     ANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3128..3898)
                     /locus_tag="ACL8CL_001540"
     CDS             complement(3128..3898)
                     /locus_tag="ACL8CL_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HGS1330767.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTIKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3913..4761)
                     /locus_tag="ACL8CL_001541"
     CDS             complement(3913..4761)
                     /locus_tag="ACL8CL_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HGS1330768.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENSNLVGLEDHPVVHVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4758..6233)
                     /locus_tag="ACL8CL_001542"
     CDS             complement(4758..6233)
                     /locus_tag="ACL8CL_001542"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HGS1330769.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDMYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
     gene            complement(6557..7912)
                     /locus_tag="ACL8CL_001543"
     CDS             complement(6557..7912)
                     /locus_tag="ACL8CL_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS1330770.1"
                     /translation="MNTMLDKMQEKLSPIAMKVGNQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGCFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
     gene            complement(7922..8239)
                     /locus_tag="ACL8CL_001544"
     CDS             complement(7922..8239)
                     /locus_tag="ACL8CL_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1330771.1"
                     /translation="MNESNLESAMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEETSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            complement(8256..8573)
                     /locus_tag="ACL8CL_001545"
     CDS             complement(8256..8573)
                     /locus_tag="ACL8CL_001545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1330772.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            complement(8588..9484)
                     /locus_tag="ACL8CL_001546"
     CDS             complement(8588..9484)
                     /locus_tag="ACL8CL_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HGS1330773.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLKRIEELGFDFVCLTGNPGTGVSNQEIIKAVQSAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            complement(9487..10749)
                     /locus_tag="ACL8CL_001547"
     CDS             complement(9487..10749)
                     /locus_tag="ACL8CL_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HGS1330774.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFITGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFI
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            11029..12207
                     /locus_tag="ACL8CL_001548"
     CDS             11029..12207
                     /locus_tag="ACL8CL_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HGS1330775.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NELIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIEPYNDFTAAHSAIALCLYHTILHS
                     "
     gene            complement(12435..12881)
                     /locus_tag="ACL8CL_001549"
     CDS             complement(12435..12881)
                     /locus_tag="ACL8CL_001549"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1330776.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGSRIPFLLTLQ
                     ALEEVFSIFDEQEFVGLIQKIRLEDSNLHIGRFFINPQKQGQGLGSQALRKFVSLAFE
                     NEDIDSISLNVFEANQRAQNLYQKEGFEIVQMVEAPVRKYSRLKLE"
     gene            12951..13610
                     /locus_tag="ACL8CL_001550"
     CDS             12951..13610
                     /locus_tag="ACL8CL_001550"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HGS1330777.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTPLR
                     PLDLIPILSQVFNRFRCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLGQVVLITAG
                     LTLSIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLALGILAHFIDIRMGAGDFL
                     FLASCALVFSITELLILIQFASATGILAFLLQKKKERLPFVPFLLLATCVIIFGKLLL
                     V"
     gene            complement(13662..14813)
                     /locus_tag="ACL8CL_001551"
     CDS             complement(13662..14813)
                     /locus_tag="ACL8CL_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1330778.1"
                     /translation="MFNGRVLKELRLLNGLSRAELAQRINLTEQAIWQFESNETKPKL
                     STKMHLANQFHVDLTYFEQEEESIRFDSSVIAFRNADLATRKTIDIQTMYLHKVDSLI
                     DYFESFVIIPNIIIHDLSNVVSESYHKGESIEELALYAREKLGISKDNHDLLYKLERS
                     GIYIVERLINGQADAYSAWSKLGRPYIVLGTNKSSVRRNFDLAHELGHILLHKYKDMN
                     EDGDRLEQEANYFASCFLLPKKEFLVKFEERVGKRVSNPDSYILLKSDLNVSIQALEY
                     RAFKLGLLTPKQHSYFYRQIAQKGYKMIEPLDDQIFVKKPSKVKSILDVVLSNHLVSL
                     ATIMSKQSIRLQFISEIFSVEMKFFDQYQEDRRTDRFDNIIPLYKRNNL"
     gene            complement(14828..15559)
                     /locus_tag="ACL8CL_001552"
     CDS             complement(14828..15559)
                     /locus_tag="ACL8CL_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330779.1"
                     /translation="MLNQDLFDSLEAQKIVDTLMKGQKDYVDERLEKRETMIVSNGYA
                     WTRPNHIDTAFASADLFEYKLQLAGQTWGYLEFETNTEKYGKVLLIIKGKKRLTNQFP
                     LVQKNKSGYLFEYAQMNTLYLNQHSSYKNDENSHSFPIQMELVSDEMIQEIEQATKNS
                     NIEKFMILTYEADSENNIISVDVVMPDARTGQLHLIQDLSEYIQSSSYHFEEAKYQDI
                     PNFSELSETEDFEIIPRIEKQEGQK"
     gene            15788..15889
                     /locus_tag="ACL8CL_001553"
                     /pseudo
     CDS             15788..15889
                     /locus_tag="ACL8CL_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            complement(15861..16637)
                     /gene="trpA"
                     /locus_tag="ACL8CL_001554"
     CDS             complement(15861..16637)
                     /gene="trpA"
                     /locus_tag="ACL8CL_001554"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HGS1330780.1"
                     /translation="MPKTLTEKLNAIKATGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIRQAVAYQK"
     gene            complement(16630..17853)
                     /gene="trpB"
                     /locus_tag="ACL8CL_001555"
     CDS             complement(16630..17853)
                     /gene="trpB"
                     /locus_tag="ACL8CL_001555"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HGS1330781.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDLTGRDLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGSPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            complement(17831..18430)
                     /locus_tag="ACL8CL_001556"
     CDS             complement(17831..18430)
                     /locus_tag="ACL8CL_001556"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HGS1330782.1"
                     /translation="MTKVKICGLSTKEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPADVKKVGVFVSPSRAELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGEGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKNGISRTK"
     gene            complement(18417..19184)
                     /gene="trpC"
                     /locus_tag="ACL8CL_001557"
     CDS             complement(18417..19184)
                     /gene="trpC"
                     /locus_tag="ACL8CL_001557"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HGS1330783.1"
                     /translation="MSQEFLARILEQKVREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINHDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALSEERLKELYDYATELGLEVLV
                     ETHNLAELEVAHRLGAEIIGVNNRNLTTFEVDLQTSVDLAPYFEEGRYYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVAQRIKELQIDKG"
     gene            complement(19181..20185)
                     /gene="trpD"
                     /locus_tag="ACL8CL_001558"
     CDS             complement(19181..20185)
                     /gene="trpD"
                     /locus_tag="ACL8CL_001558"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HGS1330784.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            complement(20196..20762)
                     /locus_tag="ACL8CL_001559"
     CDS             complement(20196..20762)
                     /locus_tag="ACL8CL_001559"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HGS1330785.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMIRDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEEGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            complement(20759..22120)
                     /gene="trpE"
                     /locus_tag="ACL8CL_001560"
     CDS             complement(20759..22120)
                     /gene="trpE"
                     /locus_tag="ACL8CL_001560"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HGS1330786.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESISRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYMVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEGMVETARDLIRNGDMFQCVLSQRFSA
                     EVTGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKTLATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMKVELFRY
                     VMHLTSVVKGRLLPELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
BASE COUNT         7047 a         4852 c         3752 g         6911 t
ORIGIN      
        1 aaattgattt tttgatatta aaataaaaaa ggagggtagg cagtgttgtg atcaattatt
       61 gctggaggtc ttattggtct cttggcaggt aaaatcacta aaaaaagtag ttctatggga
      121 atcatcgcaa atgtattcgc tggtttagtc ggggcatctg caggacaatc tcttttaggt
      181 agttggggtt catcaatcgc tggaatggct ttgctcccat ctattgtagg tgcagcgatt
      241 gtgattactg tagtgtcatt ctttacaggt agaaagtaaa cttttcgcca gtaaagttag
      301 caaactattt ttaaatcaat gacgggaaaa atagtttaaa tgttaaatcg aaaggattgt
      361 atatgtcaaa agcaaagaaa atatgtttca ttattttctg tattttaatc ttgacaattt
      421 tccttcctgt tttgatagat tatcatcaag ttagtgatct aggtattcat ctacttagct
      481 ggagacagaa ctccgtagtt gaattctatc ttgctagata tgtcttttgg gggacagtgg
      541 ttctatcaac tttagtttta ttatccattt tagttgtgat gttttatcct aaacgttact
      601 tggaaatcca acttgaaact aaaaacgata cattaaaatt aaagaattcg gcaatcgaag
      661 gttttgttag aagtttggtg agtgatcata gattgatcaa gaacccaact gttcatgtaa
      721 atttacgaaa aaataaatgt ttcgttcatg tagaaggtaa aattcttcct tcagacaaca
      781 tcgctgacag atgccaaata attcaaaatg aaataactaa tggattgaag cagttttttg
      841 gtattgagcg tcaagtaaaa cttgaagttg cagtaaaaaa ttaccaacca aaacctcaaa
      901 acaaaaagac tgttagtcgt gtgaagtaag gaagtaaaaa atggaatggc ttaaacaata
      961 tcgatatcca attatcgctg gtctcatagg cgtatttctg gcttgtttga ttgtctcctt
     1021 tggcttcttc aaaacaatat ttgtattgat tttaggagca ctgggagttg cagctggatt
     1081 atatatcgaa aaaaactata tagataaata aaaaaataaa aattactaat ttaattaaag
     1141 gagtttcata tgtcaaacga aaaaaacaca aacactaacg tagaaaagaa agatgctact
     1201 gttgtagctc acgaaatcaa aggggaactt acttacgaag ataaagttat ccaaaaaatc
     1261 attggtcttt cactagaaaa cgtttcaggt cttttgggaa tcgatggtgg tttcttctca
     1321 aatcttaaag aaaaaatcgt taacagcgat gacgtaacaa gtggtgttaa cgtagaagtt
     1381 ggtaaaacac aagttgcagt tgacttaaac gttattgttg agtaccaaaa aaatgttcca
     1441 gctttatatt cagaaatcag agaaatcgta tcttcagaag ttgctaaaat gactgacttg
     1501 gaaattgttg aaatcaacgt aaacgttgtc gacatcaaaa ctaaagaaca gcatgaagca
     1561 gactcagtaa gccttcaaga tcgcgtatct gacgttgctg aatcaacagg agaattcact
     1621 tcagaacaat tcgaaaaagc taaatctggt cttggatctg gtttctcaac tgttcaagaa
     1681 aaagttagcg aaggtgtaga agctgttaaa ggtgcagcaa atggtgtagt atctcacgaa
     1741 aacactcgtg taaactaaga taaaataaat ataacaggag aaattatcat gtcagtagaa
     1801 gaaaaattaa atcaagctaa aggttctatt aaagaaggtg ttgggaaagc catcggtgat
     1861 gaaaaaatgg aaaaagaagg agcggctgaa aaagttgttt ctaaagtaaa agaagtcgcc
     1921 gaagatgcca aagacgctgt agaaggtgct gtagaaggtg ttaaaaacat gttgagtggc
     1981 gacgataaat aaggttaaaa gttactttat ctttttggta acattagtca agatgattct
     2041 cagaaaacaa aaagctagag attcccaatt gcggaactct agctttttaa ttttgcctct
     2101 ttctcttatt atatttcagc aggttgttgg ccatgagtat gaatcccatg tcaattctca
     2161 cttgacgctt ccctctcaga tgacatctct tataacccaa acaaaccttt atctgcccaa
     2221 agacagattt catatcaatc ttacgtttag cgaaaatttg tctacccttg gaagataaaa
     2281 gtgcctgata ttctttagtt tttaaacact ggtaacgttc attcatatac agtctctttt
     2341 gaggggctga ttcaggttca taatcgcagt caacattgat ttcaaggctg tttgctttct
     2401 atctcccctt tttcatgata tattttcgta caggtgcttc aatagtttct atagtaaaat
     2461 gaaccaaaaa tagtacacgt tgcggtataa tgttcttgtg gcatattcat tagattttcg
     2521 taaacagttc tctcctattt gtgagcgaac aggtatagtc aattgattgt cagtttgaag
     2581 accaagagtc gtaaggcaaa ggatatggca gaatccatcc aagggtggct ggcacaattt
     2641 ctagtgaacc tctttaaatc tattacgttt gactgtggaa aagagttttc aaaatggaaa
     2701 gatatttcca atcatcacga tagtgagagc ttttttgcga atctaggatg tccaagacag
     2761 cggtgtctca acgaacattc aaatagactt ttaaggtgtc atgacttgcc aaagcagaca
     2821 gatttcaacg aagtcagtca agaattttag gctgggtaaa aactcaattt caggagaaaa
     2881 tgaaataaat attcccacaa taaaacgcat agtatcaaag tttttccaac acctgatatt
     2941 atacattttt taattttaaa gactttttcc cagcctcaag aaatcacatc ccacctaaat
     3001 cctttggata ccaaatacct catcaggtat tttctgagtt ggtaagactg tctagcttaa
     3061 tttgacaaat ggagggcgtt aaatccagtt tttacaggaa gtctatttaa atattttcac
     3121 actgacctta caaaatatga aataaattat ataaagtcat tcccatgaca aagaataaca
     3181 tccctttaaa acagtatctt acccaatttt caggtaagac ttttgataca accgtcccca
     3241 atacccctcc gcatatagaa gcaagaaaaa taactggaac aggtgctaaa tgatactgat
     3301 ttaaacctgt aaccacaaca attgtagcca aatgagacaa ctgggaaaag aaaataattg
     3361 caagagaata catcgttgct tcttttatcg aaatagaaaa aaacaccatc aataaagata
     3421 cattgagcgg accaccacca atccctaaaa aactagaaat caagcccaat agaagaccac
     3481 aaatcatata gaatcctatc ttatcaaatt gataagtttt tttaaaatct gttaaaacaa
     3541 atgatacaag taacatcaca aatagtaaaa tcatttgaac aatagatact agccgtactg
     3601 ataattgaga taacaaaact tgaaaaataa gatgacctag aaaccctcct aaaacagaag
     3661 aaaaggatac cgtaaaaatc aatctccatt ttatctcttt actttgtgaa aatcttttga
     3721 ttgttgaaat aatcgccatt ataaaaacag agaaactaga ataaaaagca atatcactga
     3781 ccgaatggta tccaaaacta tccataagtg gcttaattat aattccacca cccatgcctg
     3841 atatagaacc caatccacta gctattataa tgattagaaa ataaataatc tcaaccactt
     3901 tttaagtcca tcctattctt tcaaacatct aaaacctaga tggctactag tggaagtaga
     3961 aatacatcca tttctagcag ctactcggta tcgattacaa taagaacaat gacataaaaa
     4021 ggaacctcct ctgatagcat actcctcatc ttgtatccca tattttggta actcatagtc
     4081 atcttcatta aaactagcta ataaggtata tctcggattg cgacaccatt cccaaacatt
     4141 cccaatcatt tgatatagac caaaatcatt aggtaaaaat tcatagacag gagccgttcc
     4201 tataaatcca tctaaggcag tattttcata aggaaatctt ccttgccaag tattggcatg
     4261 ataatatcca ccttctaaaa gagtatctcc ccaaggatat tcagattgtc ttcctcctct
     4321 agcagcatat tcccactgtg cttcagtcgg taaagacatt ccgctccaat tacagaaagc
     4381 caatgcatct tctaaagcaa catgaactac tggatggtct tctaaaccca ctagattact
     4441 gttctcaccg tagggatgtt tccagcaagc gttcgaaact tgtaaccacc acggagcccc
     4501 agcaggatga ggatatccct ccctttctgc ctccggaacg aacaatataa aaacaaaaga
     4561 ccactcttgt cgctcagcta gtgttttata gcctgtttct ttcacaaatt gagcaaattc
     4621 ttgattcgta actggagtat ccgcaatgga aaaatccttt acactaacag cgacttgagg
     4681 accttcaaaa tcatctaaaa agccctcttc agaatttgtt cccatcataa atgtcccacc
     4741 cttgattttt ttcattttca tgattgacta tcccccgttt tcgttagagt tggcgttatt
     4801 ttagaaagtt caacaggaac taactcttca tccacaacaa aaccttcttc tctatatcta
     4861 agaaagtcta ctaatttttt cttcatctga cgaacaatag gttggtattt ttcacttgga
     4921 tacaaatcat tcatttcatg cggatctttt ttcatatcaa ataactgata atgatttaaa
     4981 acaggaaacc agataaattt ccactggtct gtcaagatat attgactaga atcttttcct
     5041 aaagcatgtt caccatgaaa ttctgttcgc cacccctcgt actgtccaaa taataaattc
     5101 ttaacactcc gtccatctag ttcatctgta gtagttcctc ctgccaaatc cacgagtgaa
     5161 ggaaaaatat cttgaatttt cactaattgc ttgattgttc ccctattacc agctatcaat
     5221 ccagccggat cgtaaataaa cgaaggaatg tggatacttc cctgataagg atatcccttt
     5281 ctaaacagat aatgttcacc aagctgatct ccatgatccg aaacaaacca gataatcgta
     5341 tctttatcat gacgaaattc ttttagggca gtcaaaaagc gactaatttg atggtctata
     5401 tgagtaatca atccaaaata agcagcaacc attcttcttt gatcatcttc ctttaatttc
     5461 cctcttaaag cataaataga cggaatttgc ttttctaata cttcccaatt cccgatatgc
     5521 aaatcgagaa attgaggcaa gcgttccata tacatatcaa aatagtattt tgggggattt
     5581 aatggagcat gtggtttttc aaaagacatt ttgagaaaaa aaggaaccgt tggatctctt
     5641 ctttgtaaaa atgaaatgga ctcactcaca acccagtttg ttggatgtaa cttctcatct
     5701 ttatcccaag gtctagcttc ccaagaatta caatccattc cgtcatcaat caaatcaaca
     5761 tcataaccaa ctttaccctt taaaaaggct agatagtcac tagcataatc aaattgactt
     5821 ccataagcct tatcatactt tctatcaaca tgtagatacc catcgtgaag taagacatga
     5881 tcaaacccaa gtctctgtct ggaaggaaaa acatgcatct taccaataca ctctgtttga
     5941 taacccatat ctttaaagac ttttggaaga gtattagtaa aattccaagg cacctcatct
     6001 tgataaccaa ctcgtccact tttatcttgg tctaagccag tcaataaagc tgccctagca
     6061 ggaacgcaag aaggaactgg agaataggca ttttcaaaat tataacctac actagccatc
     6121 atatctaatg ttggagtgct aactagctta tccttactat tcaaggataa ggcgtctgct
     6181 ctcatttgat ctacaacaat caaaataata tttggctgtt ttgtctgaac cataaaatct
     6241 cctttctaat atggcaaaag aggcacaaga agatatctac ctttactgca cccctttcta
     6301 tatcaatctc tctatataaa acaataacat tcttgttatg ttttatagaa aaatgaacta
     6361 aaatatgact aaatcgatta ggaaattcaa atcattttct agtactattt tagaagttac
     6421 agtgtactat tccaacttca ataaactata aacctttgtc taataacaat tttagtggag
     6481 ataagaaatc ctacacctaa ctcaatctta cacgtaatct atttctattt tatcacaaaa
     6541 aacgcaagta agaccattaa ctcaattcag ttttatctgc cattttcaca aatgggaaat
     6601 aagtcaagac actaataatc aaacaaacaa ctgataagat gatggcacgc caatcaaatg
     6661 ctgtagagaa gcaaccatat aaaattggag gcattaccca agtaacattt tgtgtaacag
     6721 gtgaaacaag accccagctt gttgcccagt aagctaccgt tgccatgaaa accgggctaa
     6781 gtacaaatgg tataaatagc aaaggattca agacaactgg taaaccataa tttaccggct
     6841 caccaatatt aaacagaact ggtgctagac caagtttagc aacttttcga taatgactgt
     6901 ttcttgaaaa aattaaaata gcaagtacca atcctaatcc tccaaaccag acaaacgccc
     6961 caaaagaccc acttgtccat atataaggaa tcggttcacc tttttggaaa gcatccagat
     7021 tcgctaacat agcaactcca aacagccctt ccatgatggg agccaataca tttcctccat
     7081 ggagaccaaa aaaccagaat aacttattta aaaagatcat cagaataact gcaaagaaac
     7141 tttgagacaa acctagtaat ggcgtttgta acaccttgta aacccaatca atcaataagt
     7201 cattgctaag taaatggaaa acataagtca agatggctac tatatacatc gccataaatc
     7261 ctggaatgat agaagtgaac ggcttagcaa tcgcaggggg aactgaatct ggtaacttga
     7321 ttacccagtt ctttttcatt actttacaga aaataataga ggctaaaaat ccaatcatca
     7381 tggctgtaaa gtagcctctg gcattaatat ggtttcctgg aatcacattc ccaatagtta
     7441 ccatcagatt tttaccatca aatgctagat tatcaattcc ttgtaagatc tgatctaatt
     7501 tcacatctcc tacatttgcc aaagggaaac tctttgtaac tgtacttcca atcgaaatga
     7561 caaacgaagc aagtgatacc aaaccagcag aaactgtatc aaccttgtaa atcttagcga
     7621 tattcactcc caagcaatag atgaacaaca aggaaacaat tggtatactt cccttgaata
     7681 ccaaattatt gatgtcaaca agccactgaa aggttttcgt aatactttct aggtgaaatt
     7741 gttgtggtaa atccactaga aaagcattta ataacaaagc aatggaacct gtcataataa
     7801 caggcatagt ccccacaaat gaatcacgta aagcaactaa aaacttctga ttcccaactt
     7861 tcattgcaat tggagaaagt ttttcttgca ttttatccaa cattgtattc atactaaact
     7921 cctaattttt ttcaaatcgt tcgtatactt ctaccacttc tttcgccaag tcaacaaaag
     7981 tcagactcgt cattaaatga tcttgcgcgt gtaccatcaa gaggctaatt gatgtttctt
     8041 ccccctgtgc ctctcttgtc aacatttctg tttgagcctt atgcgcctgt aataaggcag
     8101 cattcgcatc agctaatctt cgattggctt tagagaaatc cccttttttt gctgcctgaa
     8161 tagcctccat agcattactt ttggcttccc caccatacat aattagcccc attgcagatt
     8221 ctaaattact ttcattcata gcattctcct caatcttagt ctaataaact ttcagctaaa
     8281 tctaagactt tagaaccatt catcattcca taatctgtca tcggaataac cgccacagga
     8341 atctgtctat cttttagttt ttcttgaaag tcccctagta aatagcgaac ttgagggcct
     8401 aaaagcaaca catttacttc ctttgttgct acaatttctt ctgcttcagg agctggaact
     8461 gcaaaaattt ctgcatccaa ccccttatct tctgctgcct tttgcatctt tgtcactagc
     8521 agacttgtac tcatacctgc tgcacatgct aacataattg ttactttagc catagcttac
     8581 tcctttatca cctatttact gacctagcca agcgagatac tgtatgtctc actcctctaa
     8641 ttgccttgct caatgcataa atattatcga ctgttgccag tcccccatat cctgcgtcac
     8701 ctatatgttg aatatcaact ccacaaattt tatttctaag tgcaatttcc ttgatagtat
     8761 ctgtatcaga tgtttcttgg ctagtaccaa tagcactcaa tacaagacca cccttactat
     8821 gaacgagatc aacgacttca cgcaactctt ggtcatgaaa ggctggaacg gttccaactg
     8881 ctggaacaag tatcacatcc gcacctgctt ccaacaattg ctctgcgaca gaaagctctg
     8941 ccacaggctc attcactcct gccccgtgca ttttaccagc tataatcaaa cctgagaaat
     9001 tctctttagc agattgaact gctttgataa tctcttgatt gctaactcct gttccaggat
     9061 ttccagtcaa acagacaaag tcaaaaccta actcctctat tcgcttcaat gtttcaacac
     9121 tagcaacacg acctgcaaca atttcctgtg tttcctctag catctttgca gatgggtcaa
     9181 tcggttccaa atttacacca attggacaag caacaagctg gtgtaagcga tgaataacat
     9241 catcacctga actatccaaa gcataaattt taggctcaaa aacatcaaca caattcaata
     9301 aaatcatatc agctccaaaa gctctagcaa tttcagaatt agttatatcc ccaacaaatg
     9361 tctcacgagt aactacattt tcagatagaa caactctacc ttcacttgct aaaatacttt
     9421 gttttaattc ttcagcattc atctgtaata tctcagatgg atttgcacta attaaacgtt
     9481 tcattctcaa tcctccaata acttaaaccg tttttcaata gctattacaa atggtaaata
     9541 aagtaagaca tccaaaacaa tcaatacaat ttgtaataat gaaccagaaa tacttcctgt
     9601 agcaagtaaa ccacttataa caggtggcat agtccaacct ggatctgtat aagttagcgg
     9661 aaccaaacct gatgccattg ctccccacgc tactagtaag ttaaacattg gagctaagat
     9721 aaatggaact agtaacaaaa tgttcaaaac aataggaacg ccaaacattg ctggttcatt
     9781 tatattaaat aatccaggta taacagttat aggtgccaaa gtttttagtt gtttactagc
     9841 tttcttctta tgtgctaaat atcctaacgc aagaactaaa cctatggttg caccaccgcc
     9901 acctatgaat acaaaattat cgataaatac gctagtaaaa atatttggaa gagtttctcc
     9961 agtttgataa gctactttat ttgcatctag attagctaac cataacggtt tcataactgc
    10021 attaacaaca tttcctccgt gtaatcctac aaaccaaaaa ctactattaa ggaaaattaa
    10081 gataagaaga ccaataacat tatttcctaa aagtccaagt ggacctccca aagctacttg
    10141 agcaatttca tgaagattag gtaaacttaa tttatctaaa gttgcataaa caatcaacca
    10201 accaacaata gtcactgcac caggaataat ggcactaaag cttttagata ctgctggtgg
    10261 aacaccatcc ggcattttta tctgtatatt atgatttata aaccattggt agatatagcc
    10321 attaatcaat cctaaaatca tagcaacaaa taaacctttt gatgccatga aagcggtagg
    10381 cattcctgct ccagcctctc ctgtaatgaa aggcgtaact gtaataaaag atgataatga
    10441 cacaatacca gcaggaatac catcaacttt gtattgtcta gccataaagt aagcgatacc
    10501 aaaactagct acaagaccaa gtaatccaaa actactatca acacccttcc ataaataatc
    10561 agcaacacca atatcaccca gatatttttc ccatcctgga ataggaaaac ttgcaataac
    10621 cattagtaat gaacctatca acaataaagg tatagttaga gtaataccgt ctcgaataga
    10681 aaccaacgct ttattattac ccaatctagc cgctactgga agtaactttt cttgtaataa
    10741 tttttccata accttttcct cttaaatata ttttattatc aaaagttatc aattatcttt
    10801 attttttgac gtcaagttgt tatttgatta tcttttgtta tttatatttt acaactataa
    10861 gaaagccttg tcaaggattt gtgtaaatat ttgattattt tttattatca atatataata
    10921 aaaagagacc agtaagcaat gaaaagctta aaaagattaa catctacatt tatccatagc
    10981 aaaaaatcat aggattttaa gaatctgaca actaggaaga aaactaatat gaccttatct
    11041 aagaaacaat tacaactacg agctaaaata ctggaaacag tttacacatt aggtcccatt
    11101 tcacgaattg aaatcgctac taaaacagga ataacacctg caacaactag cagtatcaca
    11161 aatgagctga ttaaagaaaa cattctctta gaacttggtg aagacgaaca cgatacaagc
    11221 gtagggagaa aaaaaatcct gctagacatt caagctaaac gattttacta cattggttgt
    11281 gaactatctg aaaaacattt cacttttgct ttaggagata atctaggaaa tatcttaaag
    11341 gaagagaaag aaattgttac taaacagtta atccaagaaa agggaaatca actcattaac
    11401 cagacactaa agcaattctt aaataactgc tctgattatg aaatagaagc aattggaatc
    11461 gcgctacctg gtcgctattt agatgattat aaaataacaa caaacaatcc tttatggcaa
    11521 catatcgact tagaaatgat tcaatcccac tttgataagc ctttgttttt ctctaacaat
    11581 gttaactgta tggctatagg taaacgccta tttagtcgcc aacagaatga ccctaatttt
    11641 gcttattttc attttgctag aggtatgcac tgttcttata tctacgacgg aaacatttat
    11701 gggaaaggaa atctaatgat tggagaaata gggcatacag ttgtttcatc tgagggagaa
    11761 gaatgtagct gcgggcgcaa gggatgctta caaacttttg ctggagaatc ttggttaatc
    11821 aaaaaatcaa aaattctata tcatcaatct ccctattctc tacttcctag tttagtcaaa
    11881 aatgccgatg atattgatat ccaagttatt ttaaccgctt atcaattagg tgacactgga
    11941 ataatcacac ttatccatca agccttactc tatcttagtc aaaccatttt aaatatcagt
    12001 atgatgattg actctcaaaa aatctaccta catagtcctt tactaacaaa tcaacacatc
    12061 atccaaaaac tatatagtga aatgaactac aaacccaaac tgctctataa tcgcctacct
    12121 gaagtcatta ttgaacctta caatgatttt acagccgcac actcagctat tgcactttgt
    12181 ttgtatcata caattctaca ttcttaattt tctattacat ttaataaata ggctaaaaag
    12241 agggagtata tcataaaaag cgaataaacc tgttttctga aaatcagaat tctgttttgt
    12301 tcgctttctt tatatttgga attatactcc tatctcccct ttttcataat gtatagtgaa
    12361 atgaaataat aacaggacga atcgatcggg acagtcaaat cgatttctaa caatgtttta
    12421 gaagtagagg tgtactattc tagtttcaat ctactatatt ttcgtacagg tgcttcaacc
    12481 atttgaacga tttcaaatcc ttctttttgg taaagattct gagctctttg atttgcctcg
    12541 aagacattta gagaaatact atctatatct tcattttcaa aggccaaact aacaaatttc
    12601 cttaaagcct ggctacctaa gccttgcccc tgtttctggg ggttgataaa aaatctcccg
    12661 atatgaagat tgctgtcttc tagcctgatt ttctggataa gtcccacaaa ctcttgttca
    12721 tcaaagattg aaaagacttc ttccaaggct tgaagggtca gtagaaaagg aatccttgat
    12781 cccatccatt gttcttgaaa ggatttgcct agggagttgg accattggca tacaaattga
    12841 gcgttttcta tactcacctt ttcttcaaaa cgaattgtca tcttttcctc accatcttat
    12901 ctatgtttct ccattatact atttctccca ttttttacga atagataagt atgattgatt
    12961 tttatttttt tctcgtcggg agcattctcg cttcctttct tggtttggtc attgaccgtt
    13021 ttccagagca atccattatc agttcagcca gtcactgcga ttcctgtcag actcccttgc
    13081 gtcccttaga tttgattccg attctctcac aggtcttcaa tcgctttcgc tgtcgctact
    13141 gcaaggtccg ctatcctgtc tggtatgccc tctttgaact aggcttagga ctcctctttc
    13201 tgctttactc ttgggaattg ctttccttgg ggcaagtcgt cctaatcacc gctggtttga
    13261 ccttgagcat ctatgacttt cgccatcagg aatatccctt actggtctgg atgactttcc
    13321 acctaatcct aatagcttcc tctggctgga atctggtcat ggtctccttc cttgctcttg
    13381 gaattttggc tcattttatc gatatccgca tgggcgcagg ggatttcctc tttttagctt
    13441 cttgtgctct cgtctttagc ataacggagt tactgatctt gattcagttt gcttctgcga
    13501 cgggtatcct agcctttctc ctgcaaaaga aaaaggaaag acttcctttc gtacctttcc
    13561 tcttacttgc tacttgtgtg attatttttg gtaagctact gcttgtctga taaaatccaa
    13621 tttctgccat atgtccttca tgaaattatt tcacagttaa attataaatt atttcttttg
    13681 tacaaaggga tgatgttatc aaatcgatct gttcttctat cttcttgata ctgatcaaaa
    13741 aatttcattt cgactgaaaa tatttcgctt ataaactgta aacgaatact ttgtttagac
    13801 attatagtcg ctagactgac tagatgatta ctcaaaacga cgtccagaat actctttact
    13861 ttgcttggtt ttttaacaaa aatttgatca tccaaaggtt caatcatttt gtaacctttt
    13921 tgcgcaattt gacgataaaa gtaagaatgt tgctttggag tcaataatcc taacttaaaa
    13981 gctcgatact ctaaagcctg tatcgaaaca ttcaaatccg acttcaataa aatataacta
    14041 tcaggattgc tgacacgctt gccaaccctc tcttcaaatt tgactaaaaa ctcttttttt
    14101 ggcaataaaa aacatgatgc aaaataattt gcttcttgct ccaaacgatc gccatcttca
    14161 ttcatatctt tatatttatg taaaagaata tgtcctagct catgagctaa gtcaaaattt
    14221 cgacgtacag atgatttatt cgttcctaac acaatataag gtcttcccaa ttttgaccat
    14281 gcgctataag catcagcttg gccattaatt aatcgttcca cgatatagat gcctgaacgt
    14341 tctaatttat aaagcaaatc atgattatct tttgaaatac ctaatttttc cctggcataa
    14401 agagccaatt cctcaatgga ttctccctta tgataagatt cactcactac attacttagg
    14461 tcatgaatta taatattagg tataattaca aaactttcaa aataatcaat caaactatct
    14521 accttatgta aatacatagt ttgaatatct attgttttcc gtgttgctag gtctgcattt
    14581 ctaaaggcaa ttacagaaga atcaaatcga atgctctctt cttcctgttc aaaataagtt
    14641 aaatcaacat gaaattggtt ggccaaatgc attttggttg ataatttagg tttcgtttcg
    14701 ttggactcaa actgccaaat ggcttgttcc gttaaattaa ttctctgagc taattctgct
    14761 ctacttaaac catttaacag ccgtaattct ttcaataccc gaccattaaa catttacata
    14821 ctccttacta cttttgacct tcttgttttt ctattcttgg aataatttca aaatcttctg
    14881 tttccgataa ttctgaaaaa ttaggaatat cttgatattt agcttcttcg aaatggtacg
    14941 aacttgattg aatatactca gacaaatctt gaatcaagtg taactgacca gttcgtgcat
    15001 caggcataac aacatctaca gatataatat tgttttctga gtccgcctca taagttaaaa
    15061 tcataaattt ttcgatattc gaatttttag tagcttgttc aatttcttga atcatttcat
    15121 cagaaactaa ctccatctga attggaaagg aatgactatt ttcatcattt ttgtaggaag
    15181 aatgttgatt aagataaagt gtattcatct gagcatattc aaataagtag ccactcttat
    15241 ttttttgtac caaaggaaat tggttcgtaa gtcgcttctt accctttata attaacaata
    15301 ctttcccata tttttctgta tttgtttcaa attctaaata tccccaagtc tgtcctgcta
    15361 attgtaattt atactcaaac aaatctgctg atgcaaatgc agtatcaata tgattaggtc
    15421 gcgtccatgc ataaccattc gacactatca ttgtctctct tttttctaga cgttcatcta
    15481 cataatcttt ttgccctttc atcaaagtat ctacaatttt ttgtgcctca agcgaatcaa
    15541 agagatcctg attcaacata attcttcctc ctccaaatac tttttaatga attataccat
    15601 tttcttaaag aaattactac aataattatc tttttcttaa agttctgtgt cagagtaatt
    15661 tagaaaatta tatcttctat agtaaaatca attaaaaact gaacaaattt attgggaaat
    15721 tcaaatcgct ttctgaaaat attttaggaa ccgtagtgta atattccaga ttcaattcac
    15781 tataaaactg acctttctcc tgcaaaagaa aaaggaaaga cttcctttcg tgcctttcct
    15841 cttacttgct acttgtttga ttatttttgg taagctactg cttgtctgat aaaatcctga
    15901 atcggctctc cttggtggag agcttttact atttttgaac cgacgataac accatctgac
    15961 accgcattga agcgttccag atcggcttga ctagatacac caaaacctgt caagactggg
    16021 atgtcggcca cttgatgaag ttgcgccaag tgcttgtcca aatctgcacg gtaattgcct
    16081 gatttccctg tcactccatt gatggcaacg gcatagatga atccctccgc cccttcaatc
    16141 aactctttct gacgctcaat tcctgtggtc aagcttacta aaggaatcaa ggcgatatct
    16201 gtatctgcca aaaatggttc tacaaagttg gcatgttcat gaggcaggtc tgggataatc
    16261 aagcccttca cagctgtatc agccagatct ttgacaaagt tctccacacc gtactgaaag
    16321 agggggttga agtaggtcat gatgaccagt ggaatctctg tttcaatggt tttcaaggtt
    16381 tcaactaaag cctgggtaga ggtcccgtgg gctaaactgc gcaagccagc ttcttcgata
    16441 acaggtccat ctgcaacagg gtctgaaaag ggaataccca cttcaattgc agagacaccc
    16501 aaatcttcta aaaagtgaat tgtttcagca agaccgtcca aacctttctc gtggtcacca
    16561 gccatgatat agggaacaaa aattcctttt ccagttgctt ttatagcatt caatttttct
    16621 gttagtgtct taggcatgag cttctccctt ctttgctgca tctgcttcca agcggtcttt
    16681 gacttgaacc acatccttgt ccccacgacc tgataggcat acaatcatag acttttctgg
    16741 tccaagttct ttggccaatt tcaccgcaaa agcgatagca tggctagatt ccaaggctgg
    16801 gataatccct tccacacgag acaagagttg aaatccttcc aaagcttctt cgtctgtcac
    16861 agggacatag ctggcacgtt tgatatcgtg gtagtgagaa tgctctggac cgataccagg
    16921 atagtccaaa cctgctgaga tagagaaggc ttcaagaatt tgaccatggg catcttggag
    16981 cacatccatg agagaaccgt gaagaacacc cggactaccc ttggtcaagg tagcagcgtg
    17041 gtgctctgta tccacaccaa gccctgctgc ttcagttcca tacatagcta ctgactcatc
    17101 ttctacaaag ggatggaaga gcccgatagc attcgaccca ccaccaacac aggctactag
    17161 ggcatctggc agatctcgac ctgtcaagtc acggtactgt tgtttagcct ctcgaccgat
    17221 gacactttgg aagtcacgaa cgatttctgg aaatggatga ggccccaagg cagaaccaag
    17281 gatatagtgg gtatcgtcga tattagccac ccatgaacga agggctgcat tgaccgcatc
    17341 cttgagcacg cgcgaaccat ctgtcacggc ctcgacctta gctcccaaaa gctccatacg
    17401 gaagacattg agggcttggc gtttgacatc ttcctcaccc atgtagatgg tacattccat
    17461 gttaaagagg gccgcagcag ttgcagttgc cacaccgtgc tgaccagcac cagtttctgc
    17521 gataattttc tttttaccca tgcgtttggc aagccaaact tgtcctaagg cattgttaat
    17581 cttgtgggct cctgtatggt taaggtcttc ccgtttgaga taaatcttgg ctccgccgat
    17641 atgctgggtc aagttttttg cgtagtaaag gggagtttca cgtcccacat actggcgcaa
    17701 aagctggttt aattcctctt ggaaacttgg gtctgcctga ctttcacggt aggccttctc
    17761 caactccaaa actgctgtca tcaatgtttc tgggacaaaa cgtccgccaa attttccgta
    17821 aaatccatct ttatttggtt cttgatatgc cattctttac cctctctata aatcttctaa
    17881 tcttttcatg atctttttgt ccatctgtct ccactccgct tgatacatct actgcatagg
    17941 gagtaaagtg ttgaattgct tttactacat tatcttcatt aaggccacct gcgataaaga
    18001 agggctgtgc tagtccagtc gtatccagtt gaccccaatc aaagggctgg ccactcccag
    18061 ccacaggggc atcaaagagt agatagtctg cctgagaatt gggcacatga ccctctccat
    18121 ctacctgcac agcctgaatg ctagcacaag gcaaattctc aaataaatca tctgccacct
    18181 gaccgtgaac ttgaaccaag tccaagccaa ctttgtcaat cgcttccagc agttctgccc
    18241 gacttggtga aacaaataca ccaacctttt tgacatctgc aggaataagc tttgccaact
    18301 cagctgcctc ttctaaagtc acctgtcttt tactaggtgc aaagacaaaa ccgatatagt
    18361 cggctcctgc tgaaacggct gtttccaccg cttctttggt cgatagtcca caaattttaa
    18421 cctttgtcaa tctgcaactc cttgattctc tgggccacat tttctgcctg cataagagct
    18481 gtccctacca aaattccgtt aaagtatggg gctagtcgtt ccgcatcctg ccctgtgaaa
    18541 atggcagatt cagaaatgta atagcgacct tcctcaaagt aaggggctaa atctacactg
    18601 gtctgcaagt cgacctcaaa ggtagtcaag ttgcggttgt tgaccccgat aatctcagca
    18661 ccaagtctgt gggctacctc tagttcagct agattgtgag tctccactaa gacttccaga
    18721 ccaagctctg tcgcgtagtc atacagttcc ttgaggcgtt cttcggacaa ggctgccaca
    18781 atgagcaaga taactgtcgc acctgcattg cgagcgcgga tgatttgctt ttcatcgatg
    18841 ataaagtctt tgttgagcgt cggaatctct acctgactgg aaatttcccg tagataatcc
    18901 aaatgccctt taaagaaaac ctcatctgtc aacaccgaaa tcatcactgc tccgttttct
    18961 tcataagtct gggcctgttg cacaatatcc acatcgtgat tgatatctcc caaactaggg
    19021 ctagctttct tgacctcagc gattacctgc aagcggtcct gatgattctt caaaaactct
    19081 gccaagcgat aggtctggcg cagaggctgg atttgctcca gcttcatctg ctccacctca
    19141 cgcaccttct gctctaagat tcgtgctaaa aattcctgac tcatttttgg tactcctgta
    19201 acagtctgag tttttcaagg gccttgcctc tagcaatcac ttgacgggcc aaggcaactc
    19261 cttccttgat gctatcaatc ttaccattag catagaaacc aagaccagca ttcaagactg
    19321 tcgtttccaa gaatggactt gcttcgtttt tcagaacgct aagcaaaatt tctgcatttt
    19381 cctgagcatt cccaccacga atatcttcca tagcatagcc ttccattccc aaatcctctg
    19441 gagtaaagct tgacaagctg atttcgccat tttcaagaag tgcaatcttg gttgttccgt
    19501 tcaagccagc ttcatccaac ccttctggtc cagcaaccac gatggcacgt ttgcgaccca
    19561 tatttttcaa aacctgagct gtactttcta ggagttctgg acgactaatt ccaagaagct
    19621 gtgtttctaa agccattgga tgaatcagtg gaccagtcaa gttcataatc gttggaattc
    19681 ccaattccaa acgagctggc atgatgtatt tcatagctgg gtgcatattt ttagcgaaga
    19741 gaaagacgat tccagtttta tcaaagacct tacctagttc agctggtttg aggtcaagat
    19801 tgattcccaa ggcttcgagg acatctgcgg aaccagattt agaagaaatc gagcggttac
    19861 catgcttggc catgtgaata ccgccaccag ccaagacaaa ggctgcagtt gtggaaatat
    19921 taaaactgaa agacttgtcc ccacctgtac cacagttgtc catggcatca tgaatctcag
    19981 ttggaatatg ctgggcatgt cctctcatga cttgggcaat ggctgtgcgt tcttcaggtg
    20041 tttccccctt catcttaaga gctaagagga gagaagcaat ctgcgcttca gttacacgcc
    20101 cagttacgat acgctcaatg acatccgtca tttccacacc tgataaattt tcaaattttg
    20161 ctagtttttc aataatctct ttcatcctag tttcctcact ttacaacctt ctcgataaaa
    20221 ttccgaatag aagacaagcc gtctggcgtt ccaatgctct ctggatggta ctggaagcca
    20281 taaatcggta ggtttttatg ttgaatcccc atgatggctt ggtcatcagt cgaacgagct
    20341 gtcacttcaa agtcttctgg catttcctca atcaaaatac tgtgataacg catgaccgca
    20401 cgtccttcct caataccttg atacaaaaca gatggcgctt caaagttgat attgctctgt
    20461 ttcccatgca tgacttttgg agccaaacct agcttaccac caaagacttc tgcaatggct
    20521 tggtggccca aacaaatccc aagaatcggc ttcttgcctg caaaatcacg aatcatgtct
    20581 tccatctttc cagcatcaac tggccaacca ggaccaggag aaaagaccag accatctgct
    20641 ttttcagctt cttcatacag cttggaatca tcatttctca gaacctgaac ttctgcaaaa
    20701 ttcccaatgt attgggccaa gttataggta aaagaatcat agttgtcaat caataaaatc
    20761 atggtcttag ttctccaatt ctagtcatag attttgcttt gttaatggtt tcttggtatt
    20821 cgttttgggc gatagagtcg tagacaatcc ctgccccagc ctgcacatag gctctttgat
    20881 ttttgagaat catggttcgg atggcaatgg ccaaatccat atcacccgtc gcagacaagt
    20941 agccgattgc tcctgcatat acgccccgtt tttccgtttc cagttcatag atgcgtctca
    21001 tcgcccgaat ctttggtgct ccagaaacgg ttccagcagg aagtgtagct ttcaaggcat
    21061 ccatggccgt gagttctgga agcaaacgcc ccttgaccac gctggtcaaa tgcatgacgt
    21121 agcggaagag ctccaccttc atatacttag taacttggac actggccgtt tcagagatgc
    21181 ggccaatatc gttacgcccc aagtctacca acattcgatg ttctgctgtt tccttctcat
    21241 cagagaggag gtcagtcgcc aaggtcttgt cttcttcatc cgtagcccct cttggtcgcg
    21301 tccctgcaat cggattggtt gtcacgatgc catttttgac agaaactaaa ctttctggac
    21361 tagctccgat gatttgataa tccccaaaat catagaaata aaggtaatta gaaggattag
    21421 tcacgcggag atttctgtag aagtcaaatg gatttccagt aacttctgct gaaaaacgct
    21481 ggctgagtac acattggaac atatctccgt tacgaatcaa gtcacgagct gtttctacca
    21541 ttccctcaaa cttatgtgga gcgatatgcg gtttgaagtc taacggagat agatccaaat
    21601 cttcaaattc atttggagca ggaatgcgta attcctcaag cacttggttc aaggattttt
    21661 ccaaggcctc ttgactgcgc tcactataaa gtgcatcctc tatgacatgt atcttctcct
    21721 tcttgtggtc aaagaccata tagctctcat agacaaagaa atgcatgtct ggcgtcccaa
    21781 ttgtatcctc agggatttga ccaatttctt cataaagcga aatcatatcg taacccacaa
    21841 aaccaatggc tccaccacca aaaggtagct ctgagtggtg ctgactctta tgaatcactt
    21901 cataaaggaa atccaaggga tcccgatcaa tcacttgacc attttgatag agaaccccat
    21961 tttcaaactt aatctcaaaa actggattat aggctaggat agaaaaacga gctgtttcct
    22021 tgtctctcga aatactctct aaaataacct tatgttgccc ctttaagcgc atataagcca
    22081 agattggtga taagacatct ccatgaatga ttcgttccat tgtaatttcc ctttcagttc
    22141 tacttctagt ccgtggtgac tgtatgaaaa atccccacgc aaaataactt gcgtgaggac
    22201 gaaattcgcg gtgccacctc aattatagga tttctcctat ctctcattcc tgtctcagat
    22261 atctcctgta acaggctgtg cgataaaggg cactcccttg agaatgatgt tttcttctct
    22321 cgtttcagat gaacccaact ttacagcttt ctctgcttgt tttcagcaac cacaagctct
    22381 ctgtgagaga aagaactgta atttttccat ctattatttt ttagcttcta gtaatctgca
    22441 atcgcagcta ggtccttgcc tccacgacca gagacattga tgaagagatg ttcatctcgg
    22501 tacaccttta tactcttcga aaatctcttc aaaccgcgtc aacgtcgcct tatcgtaggt
    22561 at
//
LOCUS       DBEROU010000037        22186 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.037, whole genome shotgun sequence.
ACCESSION   DBEROU010000037 DBEROU010000000
VERSION     DBEROU010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 22186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22186
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.037"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            120..1031
                     /locus_tag="ACL8CL_001561"
     CDS             120..1031
                     /locus_tag="ACL8CL_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HGS1330787.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPVYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVAVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            1261..1959
                     /gene="dapD"
                     /locus_tag="ACL8CL_001562"
     CDS             1261..1959
                     /gene="dapD"
                     /locus_tag="ACL8CL_001562"
                     /EC_number="2.3.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047200 - tetrahydrodipicolinate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HGS1330788.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLEGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            2027..3157
                     /locus_tag="ACL8CL_001563"
     CDS             2027..3157
                     /locus_tag="ACL8CL_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HGS1330789.1"
                     /translation="MLDLIQTRRALHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLPFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPNQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVAASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            3169..3708
                     /locus_tag="ACL8CL_001564"
     CDS             3169..3708
                     /locus_tag="ACL8CL_001564"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HGS1330790.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMDFVVYDPQQLVKTAFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGHTLSTVHPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            3692..4369
                     /locus_tag="ACL8CL_001565"
     CDS             3692..4369
                     /locus_tag="ACL8CL_001565"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HGS1330791.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQVWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIVLRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(4441..5340)
                     /gene="galU"
                     /locus_tag="ACL8CL_001566"
     CDS             complement(4441..5340)
                     /gene="galU"
                     /locus_tag="ACL8CL_001566"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HGS1330792.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVDKTTDMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FQILENQAPGAGNEIQLTDAIDTLNKTQRVFAREFNGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(5362..6378)
                     /locus_tag="ACL8CL_001567"
     CDS             complement(5362..6378)
                     /locus_tag="ACL8CL_001567"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HGS1330793.1"
                     /translation="MKKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSIILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQELFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHETNIKDTIYDIMNNEFKAE
                     NEWS"
     gene            6590..7030
                     /locus_tag="ACL8CL_001568"
     CDS             6590..7030
                     /locus_tag="ACL8CL_001568"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1330794.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIVQKKESVFAK
                     IFEEYYDRLPEEERFIIPNYSYLALTNYTVQKLPEKLVEILSFW"
     gene            complement(7145..7576)
                     /locus_tag="ACL8CL_001569"
                     /pseudo
     CDS             complement(7145..7576)
                     /locus_tag="ACL8CL_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7638..8288)
                     /gene="phoU"
                     /locus_tag="ACL8CL_001570"
     CDS             complement(7638..8288)
                     /gene="phoU"
                     /locus_tag="ACL8CL_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HGS1330795.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(8303..9055)
                     /gene="pstB"
                     /locus_tag="ACL8CL_001571"
     CDS             complement(8303..9055)
                     /gene="pstB"
                     /locus_tag="ACL8CL_001571"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HGS1330796.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(9057..9872)
                     /gene="pstA"
                     /locus_tag="ACL8CL_001572"
     CDS             complement(9057..9872)
                     /gene="pstA"
                     /locus_tag="ACL8CL_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HGS1330797.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(9865..10728)
                     /gene="pstC"
                     /locus_tag="ACL8CL_001573"
     CDS             complement(9865..10728)
                     /gene="pstC"
                     /locus_tag="ACL8CL_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HGS1330798.1"
                     /translation="MIKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(10846..11721)
                     /locus_tag="ACL8CL_001574"
     CDS             complement(10846..11721)
                     /locus_tag="ACL8CL_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HGS1330799.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(11888..13219)
                     /gene="pnpS"
                     /locus_tag="ACL8CL_001575"
     CDS             complement(11888..13219)
                     /gene="pnpS"
                     /locus_tag="ACL8CL_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system histidine kinase PnpS"
                     /protein_id="HGS1330800.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(13220..13927)
                     /locus_tag="ACL8CL_001576"
     CDS             complement(13220..13927)
                     /locus_tag="ACL8CL_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1330801.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGIEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(14011..14496)
                     /locus_tag="ACL8CL_001577"
     CDS             complement(14011..14496)
                     /locus_tag="ACL8CL_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HGS1330802.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDYALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            14482..15039
                     /locus_tag="ACL8CL_001578"
                     /pseudo
     CDS             14482..15039
                     /locus_tag="ACL8CL_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(15354..17045)
                     /gene="argS"
                     /locus_tag="ACL8CL_001579"
     CDS             complement(15354..17045)
                     /gene="argS"
                     /locus_tag="ACL8CL_001579"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HGS1330803.1"
                     /translation="MNIKELIASELSSIIDSLDQEAVLKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            17181..17627
                     /gene="argR"
                     /locus_tag="ACL8CL_001580"
     CDS             17181..17627
                     /gene="argR"
                     /locus_tag="ACL8CL_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS1330804.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            17678..20212
                     /gene="mutS"
                     /locus_tag="ACL8CL_001581"
     CDS             17678..20212
                     /gene="mutS"
                     /locus_tag="ACL8CL_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HGS1330805.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDCEGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            20359..22053
                     /gene="patA"
                     /locus_tag="ACL8CL_001582"
     CDS             20359..22053
                     /gene="patA"
                     /locus_tag="ACL8CL_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HGS1330806.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELFGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
BASE COUNT         6297 a         4396 c         4845 g         6648 t
ORIGIN      
        1 gctccataat atccataggg gatttaccca ctacaaatat tatagagccc gataattctc
       61 taaatgaagt ggccaatcaa tggattgcca cttttttcct ttcgtgttac aataagggta
      121 tgaatcagta tcagaaaaag attgttaacg gaaaaattta ttcgctccta tccggcttaa
      181 tatggggaat ctgtggaatt ttaggagagt acttctttac tcattatcag gtgtcttcgg
      241 gctggattac ctctatgcgt ttgacactgg cagggagtct tgtactcatt tggtctgcaa
      301 tacaattaaa atcgcaagtg ctagatattt ggcgagacaa gaaaaattac ctgccctttt
      361 tagcttatgc tattttgggg attttttcag tccagtattt tttctatctc tgtgtagaat
      421 actcaaatgc tacgacagca actattttac agtttattag ccctgtcttt atcctctttt
      481 acaatcgctt ggtttatcaa aaacgagcgt caaaaagcgc tgttttctat gttttggttg
      541 ccatgctggg tgtttgcttg atggcgacaa agggagatct ctctcagtta tccatgacgc
      601 cgctagctct tataacaggt ttgctgagtg ccatgggtgt tatgtttaat gttatcttgc
      661 cccaaccttt tgctaagcgt tatggttttg ttcctacggt tgggtggggg atgattttgg
      721 caggtttgtt tagcaatgtc ctctcgccgg tttatcagct ttcctttact cttgatattt
      781 ggagtatctt gatttgcctc attatcgctt tctttggaac ggcttttgct tttttcattt
      841 ccatgaaggc tgtgtccttg gtttctcctt tggtggttgc cgttatcagt gccagtgaac
      901 ctctctcttc tgctctcttg agtgttttgt tcttaggatt agtagtggat tggtccctcc
      961 ttctagctat agccttgatt attttaccca tgatattttt gtctatagaa gaagcgaaag
     1021 aaagtagata aaaagtcttt tcttaattct aaaagtgtgc tatactagaa tagtcaataa
     1081 aacacgggga gatagttgtg aagagtgttt ttaggttgta tcggtagggg cttgctttta
     1141 ccgacagcac gttttatctc acatccctaa aaaatcatac ctaaaaacag acaaaaaggt
     1201 ttcaaatttg aggccttttt tggtagaata ggtatcatta taaagaatga gaggcctttt
     1261 atgactgcta caaaaatgaa tgctcaagaa attatccaat ttatcgccaa tgctgaaaag
     1321 aaaaccagtg tcaaagtaac ctttgagggg caactcgcaa ctgctgtgcc tagctctgtt
     1381 gtcaaactag gaaatgtcct attcggagac tggaaggacg tggctccgct tcttgaaggt
     1441 ttggtagaaa atcaagatta tgttgtcgag caagatgctc gtaattctgc agttcctttg
     1501 ctagataagc gtgctatcaa cgctcgtatc gagccaggtg ctattatccg tgaccaggtg
     1561 gaaattggtg acaatgctgt tatcatgatg ggagctgtta tcaatatcgg tgctgaaatc
     1621 ggtgctggaa ccatgattga catgggtgcc atccttggtg gccgtgctat cgttggaaaa
     1681 aatagccacg ttggtgcagg tgcagttttg gcaggtgtga ttgagccagc tagtgctgaa
     1741 ccagtccgtg tcggagacaa tgttcttatc ggtgctaatg cagtggttat cgaaggagtc
     1801 caaatcggta gtggttcagt tgtcgctgca ggagctatcg ttacccaaga tgtcccagaa
     1861 aacgtggtag tagcaggtgt tccagctcgt atcatcaaag aaattgatgc ccaaacccaa
     1921 caaaaaacag cgctagagga tgcgcttcgt accttgtaat tgtaaaagta aaaaagaggc
     1981 ggaacccttt ttccagcctc tttctgctat atcggaggac agatagatgt tagatttgat
     2041 tcagactaga cgagctttac accagattcc agagattggc ttggaggagt tcaagactca
     2101 ggcttatttg ctggatgtga ttgagaaatt gactacgggc aaggattttg ttcaaattcg
     2161 tacttggcgg acagggattt tggtctacct gcagggaagt cagccggagc gaaccattgg
     2221 ttggcggaca gatattgatg gcctgcctat cgtcgaacaa acaggtctac cttttgcttc
     2281 tcaacaccaa ggtcgcatgc atgcctgtgg ccatgatttt catatgacca ttgccttagg
     2341 ctgtcttgag cgcgcccttg aggagcaacc caagaataat ctgctctttc tatttcagcc
     2401 tgctgaagaa aatgaagctg gtggtatgct catgtatgag gatggtgctt ttggagattg
     2461 gttgccaaac caattctatg gtctccatgt tcgtccagat ttgaaagtcg gccagattgc
     2521 gactaatact catacactct ttgcaggaac ttgcgaggtg aagatccgtt tcaaaggcaa
     2581 aggtggccac gcagcttttc ctcatgaagc caatgacgcc ttggtggcgg ctagttactt
     2641 tgtgacccaa gtgcagtcag ttgttagccg caatgtcaac ccaatcgagg gagcagtggt
     2701 gaccttcggc gtttttcaag ctggaaccac caacaatgtc atcacagaca cagctttttt
     2761 acatggaact attcgtgcct tgacacagga catgagtctc ttggtgcaaa agagggtaaa
     2821 aacagttgca gaaggggttg cagctgcctt tgatatggaa gtcgaagtgg aactcaaaca
     2881 aggtggatac ctacctgttg agaacaatcc agccttggcg cgtgaactga tggacttctt
     2941 tgacgaaaaa gacggaatcg agttgattga tatcgaacct gctatgacag gcgaggactt
     3001 tggttatctc ctttcaaagg ttgatggcgt gatgttttgg ctaggtatcg atagtcccta
     3061 cgctcttcat caccctcaaa tgagtcctaa ggaagaagtc ttagctattg gggtggctgc
     3121 ggtgtctagt ttcctgaaaa agaaggcagc agaatagagg gcttgtatat gaaatcggaa
     3181 ttacgcaagc aagtcttgca tgaaatgaag gctttatctc aagaacaaaa acaggctata
     3241 gaccaagctt taaccgagcg aattttacaa cacccctttt atcaagaagc caaggtcatc
     3301 gcaacctacc tctctttttc tcatgagttt caaacgcggg aactgattga gcaggcgctg
     3361 aaggacggca agaaggtttt aatacccaaa acttatccca aggggcgcat ggactttgta
     3421 gtctatgatc cgcagcagtt ggtaaaaact gcctttgcct tactggagcc acagggagat
     3481 ttggaagtgg tggatgtatc tcagattgat ttgattcatg ttcctggcct ggcttttacg
     3541 acggatggat atcggattgg atatggtgga ggttattatg accgctatct ggaacatttt
     3601 tctggtcata ctttgagtac ggttcatcct tgtcaaattc aggactttat acctgaaaac
     3661 catgatattc ctgttcagga ggtattaatt gatgaaggaa atctttgata gacgttaccc
     3721 tgtgacgagt ttcttcctct tagtgacggc cttggtattt ttactaatgt tggtcactgc
     3781 aggcggaaac tttgacaggg cagatacatt atttcgattt ggagccatgt atgggccagc
     3841 tattcgcctc tttcccgagc aggtttggcg tctcttgtct gccatttttg ttcatattgg
     3901 gtgggaacat ttcattgtta atatgctttc actttattat cttggaaggc aggtagagga
     3961 gattttcggc tctaagcagt tcttttttct ctatctctta tcaggaatga tgggcaatct
     4021 ctttgttttt gtatttagtc ctaaatcctt agcagcaggc gcctctacct ctctttatgg
     4081 gctatttgcc gcgattattg ttcttcgcta tgcaactcgc aatccttata tccaacagct
     4141 agggcaatcc tatctgacac tttttgtggt taacattatt ggaagtgttc tgattccagg
     4201 aatcagccta gcaggccata tcggtggtgc agttggtggc gcatttctag cagttatctt
     4261 tccagttaga ggagaaaaac ggatgtataa caccagccag agattaggag cggtagtctt
     4321 gttcgtagga ctcgccattt tgcttttcta caagggaatg ggaatgtgac gaagttgaaa
     4381 gaaaatttgt gtaatgaaaa aagataaggc attttttgaa ccttatcttt ttattttatt
     4441 ttattccttc tcagtcaatt cttttccaag ttggatgagg taattcttca aatcatcttt
     4501 gacttgtggg tgtttgaggg cgtagtcgat ggatgttttc atgaagccaa acttgtctcc
     4561 gacatcgtaa cgagccccat tgaactcacg agcaaataca cgttgtgttt tattgagggt
     4621 gtcgattgca tctgtcagct gaatttcatt tcctgcacct ggagcttggt tttcgaggat
     4681 ttggaaaatt tcaggcgtga ggaggtagcg tccgataata gcaaggtcgc taggagcgtc
     4741 ctctggagct ggtttttcaa caaaggtttc aacactgtaa agaccatctt ttccttcgcc
     4801 ttgcggagca ataaccccgt aagcagatac ttcgtcatga gggactggca tgacagcgat
     4861 agtagacgcg tgggtacgct cgtagtcatc catgagttgt ttggtaagtg gaacagcctt
     4921 ttcgtctgtg atatccatca agtcatcacc aagcataacg acaaaaggtt catttccgac
     4981 gaaagcctta gcttgcaaaa cagcatctcc gagaccgcgt ggatgagttt ggcggataaa
     5041 atgcagacgc atgtcagttg ttttatcaac tagcttcaaa agatctgttt tccctttttc
     5101 tttgaggtta tattccaatt cgaagtttga atcgaagtgg tcctcaatag aacgttttga
     5161 tttaccagtg acaactagaa tatcttcaat acctgatttg agagcctctt ccacgataaa
     5221 ctggatagtt ggtttgtcta cgattggcaa catttctttg gcaagggcct tggttgctgg
     5281 taaaaatcga gttcctagtc cagcagcagg gatgactgcc tttctaactt ttgatgtcat
     5341 aagaatcctt tctatagagg gttaagacca ctcattttct gctttaaatt cattgttcat
     5401 gatgtcataa atggtatctt tgatattggt ttcgtgataa ataacttggt aaatggcctg
     5461 tgtaatgggc atatagactc caagttcttg ggctagttca taggctgctc gagtcgttga
     5521 aattccttcg attaccatgc ccatattagc ttctatatca gctagggatt ctcctcgtcc
     5581 gagagcatct ccagctctcc agttacgaga gtggatggaa gttcccgtta cgatcaaatc
     5641 tcccacacca gataagccgc tataggtcaa tggactggcc ccgagagcca cccctaggcg
     5701 agtaatttca gccaagcctc gagcgatgat ggctgcctta gcattatcac caaatccaag
     5761 accatgtaaa gctccagcac cgacagcaat aatattttta agagcaccag cagtttcaac
     5821 cccgataaca tccgtattgg tataaagtcg gaagtagtga ttactaaata gctcctgaac
     5881 gtattgagct gtttgtaaat ctttagaagc agcagttatt aaagttaggt cacgcacaat
     5941 ggtctcttct gcatgactag gtcctgaaac aacgacgata tcactacgga gatgttcagg
     6001 aatttcttct tcaagaatga ttgataatcg tttatggcta tcaggttcta atccctttga
     6061 tgcgtgcatg atgataacct tatggtccaa ggtttgtgca acttgctggg caacaagtcg
     6121 tgtcactttt gttgggacaa caaacaaaat tgcatccaca tctttcaatg tttctgctaa
     6181 gtcggtgtag gcaatgatat tttcgtctag aacgacatct ttaaagtagt gcttattagt
     6241 atggtgtgta ttaatttcat tgatttgctc gggaagattt ccccaaatac gtacctcgtg
     6301 tccattgtca tttaagactt gtgaaagggc ggttccccaa gaaccaggcc ccaagacggc
     6361 gacagtttgt tttttcatga tttcctcctt gatagggttc tttctcttat tctatcatat
     6421 tctaggggat tttgcacttg cattactggg gtatggtgga aaaatacaca aaaaccgaga
     6481 cgaagggata aactcttagt ctttagttaa aatgagaaga aatctgatag atagtttgat
     6541 tcagtatatg cttataatag aggtaaacaa ctcaggaggt tcttgtaggt tgcgagagtt
     6601 tggcgaaaaa attaaaagat tacgtttggc taaaaaaatc agtcgttcag aattttgtgg
     6661 tgatgagtct gaattaagta tccgtcaatt aattagaatt gaaaatggag aatccagacc
     6721 aacactaaca aagttaaaat atatcgctga acgtttgggg gttgaagatt acaagttgat
     6781 gccaagttat atagagttgg ataaggaata cctagaattg aagtatttct tgatgaggac
     6841 tcctacatac gaagatgaaa ctatcgtcca aaagaaagag agtgtttttg ctaagatttt
     6901 tgaagagtat tatgataggc tacctgagga agaaagattt atcatcccaa attattcata
     6961 tctggcacta acgaactaca cagttcaaaa attaccagaa aagctagttg aaatactgtc
     7021 cttctggtga ttttgcttaa tttctgatgt tcgagacatg attgaggaga tttgtcttga
     7081 ttttttccta aaaaagggca gactcctccc ttggtttgat gctcgatttt taaaataagg
     7141 tgacttattc tgttaggcat gtttccccct gccctctagc tctatatgga acagggagta
     7201 tcagatttat ctggcagatt gtatcaaata aggcttgaaa ctgttttgaa taggcgtata
     7261 gacttgagaa tttgagaata tgtcgtctag cagtagagac atattttccg gcaatatgaa
     7321 ggaagagacg tcgaaaacgc ttgatagtca aggcctttac ttcatcacca gctagctgct
     7381 ttaaaaagag gtagagatta taagccagac agcccatcat catacgtact tcattcttaa
     7441 tcatggtcga actatctgtc ttatccccaa agaatcctgc tttcctttct ttgataaagt
     7501 tttcagcatt ccctcgttca cgatagaatt gaaacagctc ctcactatct acactgctta
     7561 aattgttcaa atgaactttt ttacaaccat aaaaaagagc acttaatcgt actcttctct
     7621 tttaaggatt agttgaatta attcaaatcc actagttctc ctgtttctag gtagactagg
     7681 cgttcacaaa tgttagcaat gtaatcagcg aagcgttcca gatgccctat gatataaagg
     7741 tattgagttc cattgggaat tgaggtttct tggtctttca taagtccaat gatttccttt
     7801 gataaggcat aataatattg gtcaatctgt tcatcttttt gagcaatact aatagctttt
     7861 gaggcttggt gcaaaggaaa ggcaaccaat aaatcggcta gcatgctgag ggataattta
     7921 cccatttggt gtaactgttc ttcgtcaggg gctagttgat tttcttttag ttgcaaaaca
     7981 gctttggcaa tgcctgccat atggtctccc atacgttcaa ggtctgaaca agaagacatg
     8041 atgctaatca caaatcgaag gtcagacact tgtggctgct gcaaggccaa caaacgggca
     8101 caggtcaatt cgatagcgct ttgaccttgg ttgatagcat gatccttatt gataattagc
     8161 tctgccatct ccttgtcttt ggaggctaag gccagtaagg cttttgaagc tgtttcaagg
     8221 acaagttgcc ctagtcctaa aaaggattgt tctaattcat gcaattctaa gtcaaattga
     8281 tttctcatag gtttttcctt ccttatccga accgtcctga aatatagtct tctgtgcgct
     8341 gatcttttgg attggtaaac acgtcaacgg tatctccaaa ttcgcaaatt tctcctgtta
     8401 agaaaaaagc agttttatct gaaatacgtg aagcttgttg catgttatgg gtaacaatga
     8461 taatcgtata atcctttttt agttgctgaa tgaggtcttc aatttttaaa gtggagatag
     8521 ggtctaaggc tgaagtcggc tcatccatta acagaatatc aggttctact gctaaagctc
     8581 gcgcaatgca aaggcgttgc tgctgaccgc cagataagga catggcactc tttttaagat
     8641 catctttgac ttcttcccaa atggctgccc cttttaaaga tttctccact aaggcatcta
     8701 attgtttttt gtctcgaata ccatgtgtcc ttggaccata agccacgtta tcatagatag
     8761 acatggcaaa gggattaggc tgttgaaaaa ccatccctac acgcttgcgt agctgattaa
     8821 ggttgaattt gctactataa atatcttgct catctaagag gacttggcct tcaatatggc
     8881 aagaaggaac caaatcgttc atccgattaa gggtttttag aaaagttgat ttgccacaac
     8941 cagatgggcc tatcaaggca gtaatctgtc tttctggtaa ttgaatcgaa atatttttta
     9001 aggcttgaaa atccccgtaa aataagtcta ggtgtctaac tgaaaatgtt cccatactag
     9061 gaagctcctt tcacaagttt tcgagataat aagcttgata gagtatttat cattaaaaca
     9121 gtaataatca aaatcacacc ggtagcatag gcttcattga catgtagccc ctcacttgac
     9181 agcatataca tatgcagggc tagagaacga cctgaagaca tgagactact tggcgtattg
     9241 gtagaggtac ctaatgtata catgagggca gctgtctcac caacgatacg gccaatagct
     9301 agtatcactc cagctaaaat acctggcatg gcaactggta gaacaattct aaaaacagtc
     9361 cgtaacttac ctgccccaag tccataactt gcttgacgca tgctatcact aacagataaa
     9421 agggcttctt ctgttgagcg aataatgact ggcaacacca tgataactga ggttaagatt
     9481 cctgataaca gagagtattg aaaacctaag aagactacaa agaagagcat gccaaacaga
     9541 ccaaaaacaa tggaaggaat cccagacaag gtatctgagg ccaatcgcat gattttaaca
     9601 caaagggaat ctttttttgt atattccaca agataaaaac cagcaaaaat ccctatgggc
     9661 aaggctaaaa gaagagcacc aaagaccaga ataacggtgg aaataatcgc tggcataagg
     9721 gaaatgttct cagaagtata agtccaagaa aagagggata gacttagatg aggtaagcct
     9781 ttgatgagga taaaaccaat gattaaaaag agagagccaa aggttaaagc tgaaaaacaa
     9841 taaacgagaa gttttagcag gtatttactc ataagatgat tttcctttca agtaggcaaa
     9901 gtaggcatta atcaagagaa taaggaaaaa gagaactgct gaggttgcaa taagggcttc
     9961 cctatgctga cctgatgcgt aagccatttc cagaacaata ttggttgtta aggttctggt
    10021 tcctgaaaag agtccacttg gaataatcgg ctggttgcct gccaccaaaa taactgccat
    10081 ggtttcacct actgcgcgac cgattcctaa aataactgct gataaaatac cagatctcgc
    10141 agctggcaag atgacactaa aaatactccg ttcatgacta gctcctagag ccaagctacc
    10201 agaataatac gttttgggaa ctgttcggat agcagattct gacaaactga taatggttgg
    10261 caaaatcatt attcctaata gtaacgaagc ggttaggaca ctcatgccat ttcctaaaaa
    10321 gcttctaatc caaggcacca ataattgtag gccgaaaaaa ccataaacaa tagatggaat
    10381 ggccgccatc aagttgatag ctgattttaa gaagccatag acgggctttg gacaataata
    10441 aaccataaac accgatgtca agatgcctgt tggcacccca atcacaatcg ctcctaaggt
    10501 aattaataag gaaccaacga tcattggtaa aataccatag cttgccggaa tgttcgttgg
    10561 cgaccaatca ctgcctaata aaaaacgggc aaagccgtag ttagccatga aaggtaagcc
    10621 attactaaaa ataaagaaac agattagcaa aatagctaca acagctactg ttgcactcat
    10681 gaaaaaaatt gccctaaaaa ctgcttcttt gaaggcttgt tttatcacat cttgtccttt
    10741 ctagtgaaga aagtaaggga gatacgacac ctccctactt gccttcttta tcttattgta
    10801 cgatgaaacg tctgcatctc tttagagatt tatggagcaa acattttatt taatcttgtc
    10861 ccaggtggtt aatttgccac taaaaacgtc tgcaagttca gccatactga cttggcttgc
    10921 cttattgtca ttattgacca caacagcaat accgtctaaa gcaatagcat catgggtgag
    10981 actcttacct tcttcaggag ttaattccct agaaaccata ccaatatcag cggttttctc
    11041 cttaacagcg gtaatacctg ctgaagaccc attagaggta atatcaatcg taacttctgg
    11101 attttctttt ttataagctt ctgctaattt ttccattaaa gaagatactg aagtggaacc
    11161 tacaacagac aacttgcctg ataagtgttg gcttgtatat tctgtggttt cggttttagc
    11221 ttcaataaat ttattatctg tgaccacttg ttgaccttgt ttggagtgga taaagctgat
    11281 aaaatcttga cctagcttgg aaagattaga agaccaaaca atgttgaagg gacgttgaag
    11341 agggtattca ccatctaaaa ctgtgtctcg actagccttg acaccatcaa tctctaaagc
    11401 cttgacagat ttcgttaaag atcccaagga gatgtagccg atagcattag cattcccttg
    11461 aactgctgag agaacacctt ctgtactatt ttgaatcaca gctgttttgg cagtgttgtc
    11521 aattttttta tcaccgtctt ttttgagaat ccctgtgatt tctgtgaagg caccccgtgt
    11581 tccagagcca ttttctcgtg aaatcacctc aatcgttcct gaagctgact gtttggaagc
    11641 agctgactga ttgccacagg caacaagccc aaatcctgat aagccaatgg ctgcaagagt
    11701 aagcattttt ttgaatttca taataatcac ctttatctct atgtattttt cttgtgtagg
    11761 cttactacat ttatagtcta acaagtcttt gtaaaggttt atccctgatt catgtaaaga
    11821 ttgtgtaaag aatcaaaaaa agccactttt gaaaaatggc tgctcctaaa aatagcttta
    11881 aaaattatta gtcctgtgcg aaagattggt taggaagaaa aatcgtgaag caactgcctc
    11941 tgccaagctg actcgtcacc gtgacttggc cacctaataa ttgactgagt tctttgacaa
    12001 tggcaaggcc aagaccagtg ccaccagttt gtctgcttcg acctttatta actcggtaaa
    12061 aacgttcaaa aatacgatcc tgctctaatt gactaatacc aatccctgta tctgatacag
    12121 aaatcttaat gccttcgttc accttttggg tcttgacctc aattttcccc ccttgttcag
    12181 tgtaacggat ggcattggat aaaagattga gtaagatttg ggaaagtaat tgactatctg
    12241 atacgagggt gacatcatct ggcacctgca cctttagctg taaatccttc ttcttgagct
    12301 gaggttgcaa gctttgagtc aaatcctgta caaattctgc caaagaaagg gtcgtccatt
    12361 gtataggcat ttgttgagcc ttagataagg taagaagatg ctcaacaata tgctcaagac
    12421 gcaaactttc tttgtaaata atgtctagaa agtcatcctt gagcgcttct tcttcagctg
    12481 acatcccctt aatggtttca gcaaagccct taatcgaagt aactggtgtc ctcaattcat
    12541 gggaggcatt tgagacaaag gctaaattta acttttcata agttctaatc gttgttaaat
    12601 catatagcaa gacgagcaca gcttccacag attgggtggg gctaaaaacg ggaactgctg
    12661 tcacttctaa aatcaagtca ccctcatgaa acccacttac ttcttgtttt aaccttgttt
    12721 tttgatcaaa ggcttggtga actaaattcc gaatatccat ccgtttgagg tcatcaagtg
    12781 aacttatgtc gccgtccaca tcgggaaaat aatgaggcag agagcgactg gataataaca
    12841 tctgaccttg agcggaaact aaaaacgtcc ccatggttag gtgcgacaga agaacctcca
    12901 ttgtttcggc tagatccttg tattgctgat cctgttggga gactttggtt tttaggccag
    12961 acacatactg agccaaagac tttaagtctt cttgcccttt ttctaaaaag tattcactac
    13021 tggtcaagag aggttggtgc aaggtctcaa aagcaacttc ccatttccaa aggcaaaaga
    13081 gccagtagcc acctagtccc aaagaaaggg ctagaagaaa gagaccgatg cctttactga
    13141 tccaagttaa tgccatccct gcaatcagaa tgaggctaac acttagattg actagccaaa
    13201 attgaaggta gcgtttcatc tataactcct tgaacttata accataaccc cgaatggttc
    13261 gaataaattg aggggcttta ggattgtctt caattttttc cctcaactta ccaatatgaa
    13321 cgtccaccaa acgtgtttcc tgcccaaagt cataccccca gatacgttcc aaaagacgct
    13381 ctctagtcag tgtcatgttg ggatgtttca taagatagag caagagttca aattcttttg
    13441 gggtcaaact cagtaactta ttcgccttgt agacttcatg acgctcaggg tatactttca
    13501 aggtcccaaa tagccaagaa tcgtcagcga tattatctga atcatctcct tcttgttctc
    13561 ctttagttcg cctgaggaca gccttgacac gcgccagcaa ttctctaggg ctaaaaggct
    13621 tggtcaggta gtcatcagcc cctaattcca aggccaaaac cttatcaaat tcatcacttt
    13681 tcgcagaaac catcataatt ggagttttga cgcctttggc tctcagccgc ttacaaactt
    13741 ctatgccatc taattgtggt aacatgatat caagcaagat aaaatcaaag ggttctgttt
    13801 ctgccaaagc taaggccttc cgtccatttg tcaccaattg agtagaaaag ccttccttac
    13861 ttaaatggta gtcaagcaat ttcagaatgt gttcttcatc atccactaat aagacttgtt
    13921 ttgtcatcta ttatctccta ttggtaacat tataacacaa ttatcagaaa tcctaacatt
    13981 gctaaatcag attaaatttg cctatcaaga ctagtatctg gtcaaacgct caatcatctc
    14041 cttgtgctct ggataggtcg ccagtagatc taccctttca aataattcaa aatcctcaaa
    14101 ttcaaaacca ggagcaacaa gacaagaaac cagagcataa tccttatcaa ctgttgatcc
    14161 ccaaatagtg cccttaggaa cacagtagtg aagttgttgc cctttggata tgtccaggcc
    14221 taaagtgact gcttcgtagt gaccatctgc tgtaatcatg tgaacagtaa gtggggatcc
    14281 tgcatgaaaa taccagattt catctgctgt caatcggtga aaatgtgaag gattcgtttc
    14341 ttctaataag aaataaatac tggtataaag cgcccttccc ttaccagcaa ggtttatagt
    14401 gtctgaagct ttttttgttt gtctaaaata gccaccttca acatggggag ctaactctag
    14461 agttcttatc aagtcttctt tatccgtcgg agccaatggg ttgaagtaac tcttgttcaa
    14521 agtggtttta cgatttcaag aactcctctc agttctgagg acacggtaat gattgatgcg
    14581 acggaagtac aaatcaatcg ccctaaaaaa agaattagcg aattattctg gtaaaaaaaa
    14641 tgccacgcta tgaaggctca agcgattgtc acaagtcaag ggagaattgt ttctttggat
    14701 atcgctgtga actattgtca tgatatgaag ttgttcaaaa tgagtcgcag aaatatcgga
    14761 caagctggta aaatcttggc tgacagtggt tatcaagggc tcatgaagat atatcctcaa
    14821 gcacaaactc cacgtaaatc cagcaaactc aagccactaa cagttgaaga taaagcctat
    14881 aaccatgcgc tatccaagga gagaagcaag gttgagaaca tctttgccaa agtaaaaacg
    14941 tttaaaatga tttcaacaac ctatcgaaat catcgtaaac acttcggatt acgaatgaat
    15001 ttgattgctg gcattatcaa tcatgaacta ggattctagt tttgcaggaa gtctattatt
    15061 tggttaggtg aattagtgaa gcgtttaggc aagtgtctct ggttacgacg tcatggactc
    15121 taaatcgatt atatttaggg gtcatgacta gtgaagcagt tagctagttc gcatataagc
    15181 ggctagcgtc taacaattag gaactttagt tccaataact ttaagattac gacgttttag
    15241 gacataaatc gatcatattt atgtcctaaa actagtgaag cgcctagcca aagtccgaat
    15301 aggatttggc gttagttact tagattgctt tgcaatcaag taactttggc gatttacatc
    15361 ttctctggcg cttctactcc aagcaagcga agggcttctt tgagaacgac tgcggttgcg
    15421 tagctgaggg ctagacggct gtcgcgttct gggctttcat ccaagatacg tgtatgtgca
    15481 tagtatttgt taaaggattg agccaggcta attgcaaatt tagcaatgat agaaggttca
    15541 aagttatctg ccgcacggtt gataatacgt gggaagtctt gaatgagttt aatgatttcc
    15601 cagctttcag tatcattcaa gctatagttg ccagctgttt ctggtttgaa atcggctttg
    15661 cgtaagatag attggatacg agcgtaggca tattgaacgt aaggtccagt ttcaccctcg
    15721 aaggatacca tagcctctag gtcgaagtcg tatccatttg tacggtcggt tttgaggtca
    15781 tagaatttaa tggctccaac cccaacagca tgtgctactt ggtctttgtt ttctagttca
    15841 ggatttttag cctcgatttg gaccttggca cggctaacag cctctgcaac agtaggctct
    15901 agcaagatga cattcccttt acgagtagag agcttcttcc cttcttttgt aaccagacca
    15961 aaaggaacgt gggtaatgtc gtcactccaa tcgtagccca tctcttgcaa gacagctttg
    16021 agttgtttaa agtgggcaga ttgctcttga ccaacgacgt agatagattt agcaaattgg
    16081 tattcatttt tacggtagag ggctgcagcc aagtcacgtg tgatatagag agttgcacca
    16141 tcagacttct tgatgagagc tggatgttca attccatatt tctcaagatt cacaacttgg
    16201 gcaccttctg attcaagaag tagtcctttt tcagaaagaa tgtctacaac tgcatccatc
    16261 ttatcattgt agaaggcttc tccgttatag ctgtcaaatt caaccttcaa ttcattgtaa
    16321 aggcggttaa attccactaa actttcatcg cggaaccatt gccaaagagc gagagcttcc
    16381 tcatctccat tttcaagttt acggaaccat tcgcgcgctt cttcatccaa gctagggtca
    16441 ttttcagctt cagcgttgat gcggacatag agtttaagga gttcatcgat tggatgagct
    16501 tttacagctt cttcgtcgcc ccatttttta taggcaacaa tcaacatccc aaattgttta
    16561 ccccagtctc ccaaatggtt gaccttgacc gtttgataac cgattttttg gaaaatatgt
    16621 gacaagctat ctccgataac agttgaacgc aggtggccaa tagaaaatgg tttagcgata
    16681 ttcggactag acatgtcgat aacaacattt tcttgtttac caatattttg gtcagcatag
    16741 tgttcttttt cagtggtaac agcttgcaat acttgagcag aaatggcaga tttatcaagg
    16801 aaaaagttaa cgtaaggtcc tgttgcgaca actttttcaa aggcttggct gttcattttt
    16861 tcagccagtt cagccgcaat catttgtggt gctttacgtt cgacttttgc aagagaaaaa
    16921 gcagggaaag caatgtctcc catttctgag tttttagggg tttccagtaa ctttaaaaca
    16981 gcctcttggt ccaggctatc aatgatgcta gataattcgc tagcaatcaa ttcttttata
    17041 ttcattaaga gctccttttt ggacttttct actattttat cacaatttta aagaaagaag
    17101 aaaaaatttt tgaaatctcc tgtttttttg gtataatatg gttataaata tagttataaa
    17161 tatgcacgca agaggatttt atgagaaaaa gagatcgtca tcagttaata aaaaaaatga
    17221 ttactgagga gaaattaagt acacaaaaag aaattcaaga tcggttggag gcgcacaatg
    17281 tttgtgtgac gcagacaacc ttgtctcgtg atttgcgcga aatcggcttg accaaggtca
    17341 agaaaaatga tatggtgtat tatgtactag taaatgagac agaaaagatt gatttggtgg
    17401 aatttttgtc tcatcattta gaaggtgttg caagagcaga gtttaccttg gtgcttcata
    17461 ccaaattggg agaagcctct gttttggcaa atattgtaga tgtaaacaag gatgaatgga
    17521 ttttaggaac agttgctggt gccaatacct tattggttat ttgtcgagat cagcacgttg
    17581 ccaaactcat ggaagatcgt ttgctagatt tgatgaaaga taagtaaggt cttgggagtt
    17641 gctctcaaga cttatttttg aaaaggagag acagaaaatg gcgatagaaa agctatcacc
    17701 cggcatgcaa cagtatgtgg atattaaaaa gcaatatcca gatgcttttt tgctctttcg
    17761 gatgggtgat ttttatgaat tattttatga ggatgcggtc aatgctgcgc agattctgga
    17821 aatttcctta acgagtcgca acaagaatgc cgacaatccg atccctatgg cgggtgttcc
    17881 ctatcattct gcccaacagt atatcgatgt cttgattgag cagggttata aggtggctat
    17941 cgcagagcag atggaagatc ctaaacaagc agttggggtt gttaaacgag aggttgttca
    18001 ggtcattacg ccagggacag tggtcgatag cagtaagccg gacagtcaga ataatttttt
    18061 ggtttccata gactgcgaag gcaatcaatt tggcctagct tatatggatt tggtgacggg
    18121 tgacttttat gtgacaggtc ttttggattt cacgctggtt tgtggggaaa tccgtaacct
    18181 caaggctcga gaagtggtgt tgggttatga cttgtctgag gaagaagaac aaatcctcag
    18241 ccgccagatg aatctggtac tctcttatga aaaagaaagc tttgaagacc ttcatttatt
    18301 ggatttgcga ttggcaacgg tggagcaaac ggcatctagt aagctgctcc agtatgttca
    18361 tcggactcag atgagggaat tgaaccacct caaacctgtt atccgctacg aaattaagga
    18421 tttcttgcag atggattatg cgaccaaggc tagtctggat ttggttgaga atgctcgctc
    18481 aggtaagaaa caaggcagtc ttttctggct tttggatgaa accaaaacgg ctatggggat
    18541 gcgtctcttg cgttcttgga ttcatcgccc cttgattgat aaggaacgaa tcgtccaacg
    18601 tcaagaagta gtgcaggtct ttctcgacca tttctttgag cgtagtgact tgacagacag
    18661 tctcaagggt gtttatgaca ttgagcgctt ggctagtcgt gtttcttttg gcaaaaccaa
    18721 tccaaaggat ctcttgcagt tggcgactac cttgtctagt gtgccacgga ttcgtgcgat
    18781 tttagaaggg atggagcaac ctactctagc ctatctcatc gcacaactgg atgcaatccc
    18841 tgagttggag agtttgatta gcgcagcgat tgctcctgaa gctcctcatg tgattacaga
    18901 tgggggaatt atccggactg gatttgatga gactttagac aagtatcgtt gcgttctcag
    18961 agaagggact agctggattg ctgagattga ggctaaggag cgagaaaact ctggtatcag
    19021 cacgctcaag attgactaca ataaaaagga tggctactat tttcatgtga ccaattcgca
    19081 actaggaaat gtgccagccc actttttccg caaggcgacg ctgaaaaact cagaacgctt
    19141 tggaaccgaa gaattagccc gtatcgaggg agatatgctt gaggcgcgtg agaagtcagc
    19201 caacctcgaa tacgaaatat ttatgcgcat tcgtgaagag gtcggcaagt acatccagcg
    19261 tttacaagct ctagcccaag gaattgcgac ggttgatgtc ttacagagtc tggcggttgt
    19321 ggctgaaacc cagcatttga ttcgacctga gtttggtgac gattcacaaa ttgatatccg
    19381 gaaagggcgc catgctgtcg ttgaaaaggt tatgggggct cagacctata ttccaaatac
    19441 gattcagatg gcagaagata ccagtattca actggttaca gggccaaaca tgagtgggaa
    19501 gtctacctat atgcgtcagt tagccatgac ggcggttatg gcccagctgg gttcctatgt
    19561 tcctgctgaa agcgcccatt taccgatttt tgatgcgatt tttacccgta tcggagcagc
    19621 agatgacttg gtttcgggtc agtcaacctt tatggtggag atgatggagg ccaataatgc
    19681 catttcgcat gcgaccaaga actctctcat tctctttgat gaattgggac gtggaactgc
    19741 aacttatgac gggatggctc ttgctcagtc catcatcgaa tatatccatg agcacatcgg
    19801 agctaagacc ctctttgcga cccactacca tgagttgact agtctggagt ctagtttaca
    19861 acacttggtc aatgtccacg tggcaacttt ggagcaggat gggcaggtca ccttccttca
    19921 caagattgaa ccgggaccag ctgataaatc ctacggtatc catgttgcca agattgctgg
    19981 cttgccagca gaccttttag caagggcgga taagattttg actcagctag agaatcaagg
    20041 aacagagagt cctcctccca tgagacaaac tagtgctgtc actgaacaga tttcactctt
    20101 tgatagggca gaagagcatc ctatcctagc agaattagct aaactggatg tgtataatat
    20161 gacacctatg caggttatga atgtcttagt agagttaaaa cagaaactat aaaaccaaga
    20221 ctcactagtt aatctagctg tatcaaggag acttctttga caattctcca cttttttgct
    20281 agaataacat cacacaaaca gaatgaaaag gagctgacgc attgtcgctc ccttttgtct
    20341 attttttaag gagaaagtat gctgattcag aaaataaaaa cctacaagtg gcaggccttg
    20401 gcttcgctcc tgatgacagg cttgatggtt gctagttcac ttctgcaacc gcgttatctg
    20461 caggaagtat tagacgccct ccttactggg aaatatgaag ctatttatag tatcggggct
    20521 tggttgattg gtgtggccgt agtcggtcta gttgctggtg gactcaatgt tgtcctcgca
    20581 gcctatattg cccaaggagt ttcatccgac cttcgggagg atgccttccg taaaattcaa
    20641 accttttctt atgctgatat tgaacaattt aatgcgggaa atctagtcgt tcgaatgaca
    20701 aatgatatca accagattca gaacgttgtc atgatgacct tccaaattct tttcagactt
    20761 cccctcttgt tcatcggttc gtttatccta gcggttcaaa ccttaccttc tctgtggtgg
    20821 gtgattgttc tcatggtagt cttgattttt ggtttgactg ctgtcatgat gggaatgatg
    20881 gggcctcgtt ttgccaagtt tcaaaccctt cttgagcgca tcaatgccat tgccaaggaa
    20941 aatttacgtg gcgttcgtgt ggtcaagtcc tttgtccaag aaaaagagca atttgctaag
    21001 tttacagagg tctcagacga gctttttggt caaaaccttt acattggtta tgccttttca
    21061 gtagtggaac cctttatgat gttggttggt tacggggcgg tcttcctctc tatttggctg
    21121 gtcgcgggaa tggttcagtc ggatccgtct gttgttggtt ccatcgcttc ttttgttaat
    21181 tacctaagcc agattatctt taccattgtt atggttggat ttttgggaaa ttctgtcagc
    21241 cgtgccatga tttccatgcg tcgtattcga gaaattcttg acgcagagcc agctatgacc
    21301 ttcaaggata tcccagatga agagttggtt ggaagcctta gctttgaaaa tgtgaccttt
    21361 acctatccaa tggacaagga accgatgctg aaagatgtga gctttactat tgaacctggt
    21421 caaatggttg gtgtagttgg agcgactggt gcaggaaagt caaccttggc tcaattgatt
    21481 ccacgtctct ttgatccaca gaacggggcc attaaaatcg gtggcaagga tattcgagaa
    21541 gtgagtgaag gaaccctgcg taaaacagtt tccatcgttc tccaacgtgc cattcttttt
    21601 agtggaacga ttgcagataa cttgagacag gggaagggaa atgctactct atttgaaatg
    21661 gagcgcgcag ccaatattgc ccaggctagt gaattcattc atcgtatgga gaaaaccttt
    21721 gaaagtccag ttgaagaacg gggaaccaat ttctctggtg gacaaaaaca aaggatgtcg
    21781 attgcgcgtg ggattgtcag caatccacgt attctgattt ttgacgattc gacctcagcc
    21841 ttggatgcca aatcagagcg cttggtgcaa gaagctttga ataaggactt gaaggggacg
    21901 acaaccatta ttattgctca aaaaattagc tcggttgtcc atgcagacaa gatcttggtt
    21961 ctaaatcaag gacgattgat tggtcaaggc acgcatgcag acttggttgc caacaatgcc
    22021 gtttaccgtg aaatctatga aacacagaaa tgaaagacaa actataagaa aagtcaatag
    22081 ttttatctaa attatttctt atttcaattt gatgatttgg cgatgatttt agagcacggc
    22141 aaaaagccct tgaaaaagtc cattttttca aaggtaatcc tgtgtt
//
LOCUS       DBEROU010000038        21668 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.038, whole genome shotgun sequence.
ACCESSION   DBEROU010000038 DBEROU010000000
VERSION     DBEROU010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21668
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.038"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            198..2180
                     /locus_tag="ACL8CL_001583"
     CDS             198..2180
                     /locus_tag="ACL8CL_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1330807.1"
                     /translation="MKSSKLLALAGVTLLAATTLAACSGSGSSAKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDEQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPSSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGSLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNLFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEEFNKKAQEDLAKHVK"
     gene            2481..7784
                     /locus_tag="ACL8CL_001584"
     CDS             2481..7784
                     /locus_tag="ACL8CL_001584"
                     /EC_number="3.2.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 -
                     endo-alpha-N-acetylgalactosaminidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpGH101 family
                     endo-alpha-N-acetylgalactosaminidase"
                     /protein_id="HGS1330808.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STANLPADLATALATAKENDGRDFEAPKVGEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPVAAKPETPKTVTPEWQTVEKKEQQGTVAIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKEDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYDDERTVVSVKTDNQEGVKTEDTPAEKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYSLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKLRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPQYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTEKEKMYYFNTQAGTTTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRNNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDEAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKVTFLVTGAETGD
                     TWVGIYSTGNASNTRSDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FASLTAPAQAQEGLANAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTATDWPDNNKSKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKTPDYKQEIA
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(7951..10110)
                     /gene="pbp1a"
                     /locus_tag="ACL8CL_001585"
     CDS             complement(7951..10110)
                     /gene="pbp1a"
                     /locus_tag="ACL8CL_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP1A"
                     /protein_id="HGS1330809.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLTYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWNSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(10107..10703)
                     /gene="recU"
                     /locus_tag="ACL8CL_001586"
     CDS             complement(10107..10703)
                     /gene="recU"
                     /locus_tag="ACL8CL_001586"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HGS1330810.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYNGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            10769..11296
                     /locus_tag="ACL8CL_001587"
     CDS             10769..11296
                     /locus_tag="ACL8CL_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HGS1330811.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDQLNELAENFSEN"
     gene            11366..11695
                     /gene="gpsB"
                     /locus_tag="ACL8CL_001588"
     CDS             11366..11695
                     /gene="gpsB"
                     /locus_tag="ACL8CL_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HGS1330812.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            11716..12096
                     /gene="rnpB"
                     /locus_tag="ACL8CL_001589"
     ncRNA           11716..12096
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACL8CL_001589"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            12181..13338
                     /locus_tag="ACL8CL_001590"
     CDS             12181..13338
                     /locus_tag="ACL8CL_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HGS1330813.1"
                     /translation="MKKEFNLIATAAAGLEAVVGREVRELGYDCQIENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            13351..14721
                     /gene="mapZ"
                     /locus_tag="ACL8CL_001591"
     CDS             13351..14721
                     /gene="mapZ"
                     /locus_tag="ACL8CL_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HGS1330814.1"
                     /translation="MSKKRRNRQKKEGQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLNQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKPAIV
                     DGVLDANAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQASSNTTS
                     EPKPSSSNETRSSRSEVNMGLSSEGVAVQRSASRVAYNQSAIDDSNNSAWDFADGVLE
                     QILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGYFVGNGAGH
                     ADDLDY"
     gene            14797..16221
                     /gene="gndA"
                     /locus_tag="ACL8CL_001592"
     CDS             14797..16221
                     /gene="gndA"
                     /locus_tag="ACL8CL_001592"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HGS1330815.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVSDVLEEIS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEDQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            16233..16922
                     /locus_tag="ACL8CL_001593"
     CDS             16233..16922
                     /locus_tag="ACL8CL_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1330816.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVFIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            17020..17985
                     /gene="cbpC"
                     /locus_tag="ACL8CL_001594"
     CDS             17020..17985
                     /gene="cbpC"
                     /locus_tag="ACL8CL_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HGS1330817.1"
                     /translation="MKLLKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDIGEMVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYAVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKLAPWYYLDPETGIM
                     RTGWQYLGNNWYYLRSSGAMATGWKYVRGKWYYLDNKNGDMKTGWYQDGSTWYYLDPS
                     NGDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            18004..19002
                     /gene="cbpJ"
                     /locus_tag="ACL8CL_001595"
     CDS             18004..19002
                     /gene="cbpJ"
                     /locus_tag="ACL8CL_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HGS1330818.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKELEIKTQDSVGRKYGEKRENSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYIGGERRAGWINDASAWYYLDPATGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDHPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNMTVDGYSVNYNGEWVR"
     gene            complement(19120..20352)
                     /locus_tag="ACL8CL_001596"
     CDS             complement(19120..20352)
                     /locus_tag="ACL8CL_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1330819.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEVVPLLSQLFLGILGDFQENRVKHALWIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDDLHDAMGFRQSLMRVVRIVANLAGA
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKRIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLFSLLSIIMS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQAYIVIFVTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVKFNTHN"
     gene            complement(20363..20815)
                     /locus_tag="ACL8CL_001597"
     CDS             complement(20363..20815)
                     /locus_tag="ACL8CL_001597"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330820.1"
                     /translation="MFYNDLHTFHTELKKLLEKVTSNTENLGNLQLSWCKGISGIILY
                     LCMYDCDGNKDIISKYQEFVFNHHLKMMTGYCHGITSLLQTTVYNQNKLLMKKIQQVI
                     LACSERDDHGLLMFQGDSGKADLFDFGIGSMGVYWCLLNNKFPFDVQT"
     gene            complement(20883..21271)
                     /locus_tag="ACL8CL_001598"
                     /pseudo
     CDS             complement(20883..21271)
                     /locus_tag="ACL8CL_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
BASE COUNT         6776 a         3879 c         4858 g         6155 t
ORIGIN      
        1 aaatctccag attaggaact atccgtgagt tctctagtct ggagattttt caatatactt
       61 cgttattgga cggttacgat attcatattt tttgcaaaga tgttgtttga aaaataattt
      121 tcaaaaattc tgaaaattct gttgacaact ttctgaaaag agtctataat ggagagaaag
      181 ttttaaagga gaaaatgatg aaaagttcaa aactacttgc ccttgcgggc gtgacattat
      241 tggcggcgac tactttagct gcatgctctg gatcaggttc aagcgctaaa ggtgagaaga
      301 cattctcata catttatgag acagaccctg ataacctcaa ctatttgaca actgctaagg
      361 ctgcgacagc aaatattacc agtaacgtgg ttgatggttt gctagaaaat gatcgctacg
      421 ggaactttgt gccgtctatg gctgaggatt ggtctgtatc caaggatgga ttgacttaca
      481 cttatactat ccgtaaggat gcaaaatggt atacttctga aggtgaagaa tacgcggcag
      541 tcaaagctca agactttgta acaggactaa aatatgctgc tgataaaaaa tcagatgctc
      601 tttaccttgt tcaagaatca atcaaagggt tggatgccta tgtaaaaggg gaaatcaaag
      661 atttctcaca agtaggaatt aaggctctgg atgaacagac agttcagtac actttgaaca
      721 aaccagaaag cttctggaat tctaagacaa ccatgggtgt gcttgcgcca gttaatgaag
      781 agtttttgaa ttcaaaaggg gatgattttg ccaaagctac ggatccaagt agtctcttgt
      841 ataacggtcc ttatttgttg aaatccattg tgaccaaatc ttctgttgaa tttgcgaaaa
      901 atccgaacta ctgggataag gacaatgtgc atattgacaa agttaaattg tcattctggg
      961 atggtcaaga taccagcaaa cctgcagaaa actttaaaga tggtagcctt acagcagctc
     1021 gtctctatcc aacaagtgca agtttcgcag agcttgagaa gagtatgaag gacaatattg
     1081 tctatactca acaagactct attacgtatc tagttggtac aaatattgac cgtcagtcct
     1141 ataaatacac atctaagacc agcgacgaac aaaaggcatc gactaaaaag gctctcttaa
     1201 acaaggattt ccgtcaggct attgcctttg gttttgatcg tacagcctat gcctctcagt
     1261 tgaatggaca aactggagca agcaaaatct tgcgtaatct ctttgtgcca ccaacatttg
     1321 ttcaagcaga tggtaaaaac tttggcgata tggtcaaaga gaaattggtc acttatgggg
     1381 atgaatggaa ggatgttaat cttgcagatt ctcaggatgg tctttacaat ccagaaaaag
     1441 ccaaggctga atttgctaaa gctaaatcag ccttacaagc agaaggtgtg acattcccaa
     1501 ttcatttgga tatgccagtt gaccaaacag caactacaaa agttcagcgc gtccaatcta
     1561 tgaaacaatc cttggaagca actttaggag ctgataatgt cattattgat atccaacaac
     1621 tacaaaaaga cgaagtaaac aatattacat attttgctga aaatgctgct ggcgaagact
     1681 gggatttatc agataatgtc ggttggggtc cagactttgc cgatccatca acctaccttg
     1741 atatcatcaa accatctgta ggagaaagta ctaaaacata tttagggttt gactcagggg
     1801 aagataatgt agctgctaaa aaagtaggtc tatatgacta cgaaaaattg gttactgagg
     1861 ctggtgatga ggctacagat gttgctaaac gctatgataa atacgctgca gcccaagctt
     1921 ggttgacaga tagtgctttg attattccaa ctacatctcg tacagggcgt ccaatcttgt
     1981 ctaagatggt accatttaca ataccatttg cattgtcagg aaataaaggt acaagtgaac
     2041 caatcttata taaatacttg gaacttcaag acaaggcagt cactgtagat gaataccaaa
     2101 aagctcagga aaaatggatg aaagaaaaag aagagtttaa taaaaaggct caagaagatc
     2161 tcgcaaaaca tgtgaaataa ctgttgcaaa atataagaaa ggatttagta tttctcttga
     2221 atgctgaatc cttttttaca tttgtaaaga aagattctaa atgtactgac ccccaaaagt
     2281 tggagcctct ttttgtcaga atagagaaaa tttttgttaa ttttacttgt ttcctattgc
     2341 tttctcagct attatttgtt atattaaaag cataattatt ttttatttat cagggttaag
     2401 cattgcactt tcagaggaag gagtattttt taaaaagaaa atgtaaacgc ttgctcaaaa
     2461 atgaaaggat ttagaagttt atgaataaag gattatttga aaaacgttgt aaatatagta
     2521 ttcggaaatt ttcattaggt gttgcttctg ttatgattgg agctgcattc tttgggacaa
     2581 gtccggttct tgcagatagc gtgcagtctg gttccacggc gaacttacca gctgatttag
     2641 ctactgctct tgcaacagca aaagagaatg atgggcgtga ttttgaagcg cctaaggtgg
     2701 gagaagacca aggttctcca gaagttacag atggacctaa gacagaagaa gaactattag
     2761 cacttgaaaa agaaaaaccg gctgaagaaa aaccaaaaga ggataaacct gtagctgcta
     2821 aacctgaaac acctaagacg gtaacccctg aatggcaaac ggtagagaaa aaagaacaac
     2881 agggaacagt cgctatccga gaagaaaaag gtgtccgcta caaccaacta tcctcaactg
     2941 ctcaaaatga taacgcaggc aaaccagccc tgtttgaaaa gaagggcttg accgttgatg
     3001 ccaatggaaa tgcaactgtt gatttaacct tcaaagaaga ttctgaaaag ggcaaatcac
     3061 gctttggtgt cttcttgaaa tttaaagata ccaataataa tgtttttgtc ggttatgaca
     3121 aggatggctg gttctgggag tataaatctc caacaactag cacttggtat agaggtagtc
     3181 gtgttgctgc tcctgaaaca ggatcaacaa accgtctctc tatcactctc aagtcagacg
     3241 gtcagctaaa tgccagcaat aacgatgtca atctctttga cacagtgact ctaccagctg
     3301 cggtcaatga ccatcttaaa aatgagaaga agattcttct caaggcgggc tcttatgacg
     3361 atgagcgaac agttgttagc gttaaaacgg ataaccaaga gggggtaaaa acagaggata
     3421 cccctgctga aaaagaaaca ggtccggttg ttgacgatag caaggtgact tatgacacga
     3481 ttcagtctaa ggtcctcaaa gcagtgattg accaagcctt ccctcgtgtc aaggaataca
     3541 gcttgaatgg acatactttg ccaggacagg tgcaacagtt caaccaagtc tttatcaata
     3601 accaccgaat cacccctgaa gtcacttata agaaaatcaa tgagacaaca gcagagtact
     3661 tgatgaagct tcgcgatgat gctcacttaa tcaatgcgga aatgacagta cgcttgcaag
     3721 ttgtggacaa tcaattgcac tttgatgtga ccaagattgt caaccacaat caagtcactc
     3781 caggtcaaaa gattgatgac gaaagaaaac tactttcttc tattagtttc ctcggcaatg
     3841 ctttagtctc tgtttctagt gatcaaactg gtgctaagtt tgatggggca actatgtcaa
     3901 acaatacgca tgtcagtgga gatgatcata tcgatgtaac caatccaatg aaagacctag
     3961 ccaagggtta catgtatgga tttgtttcta cagataagct tgctgctggt gtttggagta
     4021 actctcaaaa cagctatggt ggtggttcga atgactggac tcgtttgaca gcttataaag
     4081 aaacagtcgg aaatgccaac tatgtaggaa tccacagctc tgaatggcaa tgggaaaaag
     4141 cttataaggg cattgttttc ccagaataca cgaaggaact tccgagtgct aaggttgtta
     4201 tcactgaaga tgccaatgca gacaagaaag ttgattggca agatggtgcc attgcttatc
     4261 gtagcattat gaacaaccct caaggttggg aaaaagttaa ggatatcaca gcttaccgta
     4321 tcgcgatgaa ctttggttct caagcacaaa atccattcct tatgaccttg gatggtatca
     4381 agaaaatcaa tctccacaca gatggtcttg ggcaaggtgt tctccttaaa ggatatggta
     4441 gcgaaggcca tgactctggt cacttgaact atgctgatat tggtaagcgt atcggtggtg
     4501 tcgaagactt caagactcta attgagaagg ctaagaaata tggagctcat ctaggtatcc
     4561 acgttaacgc ttcagaaact tatcctgagt ctaaatactt caatgaaaaa attctccgta
     4621 agaatccaga tggaagctat agctatggtt ggaactggct agatcaaggt atcaacattg
     4681 atgctgccta tgacctagct catggtcgtt tggcacgttg ggaagatttg aagaaaaaac
     4741 ttggtgacgg tctcgacttt atctatgtgg acgtttgggg taatggtcaa tcaggtgata
     4801 acggtgcctg ggctacccac gttcttgcta aagaaattaa caaacaaggc tggcgctttg
     4861 cgatcgagtg gggccatggt ggtgagtacg actctacctt ccatcactgg gcagctgact
     4921 tgacctacgg tggctacacc aataaaggta tcaacagtgc catcacccgc tttatccgta
     4981 accaccaaaa agatgcttgg gttggggact acagaagtta tggtggtgca gccaactatc
     5041 cactgctagg tggctacagc atgaaagact ttgaaggctg gcaaggaaga agtgactaca
     5101 atggctacgt gactaacctc tttgctcatg acgtcatgac taagtacttc caacacttca
     5161 ctgtaagtaa atgggaaaat ggtacaccgg tgactatgac cgataacggt agcacctata
     5221 aatggactcc agaaatgcga gtggagttgg tagatgctga caataataaa gtagttgtaa
     5281 ctcgtaagtc aaatgatgtc aatagcccac aatatcgcga acgtacagta acgctcaacg
     5341 gacgtgtcat acaagatggt tcagcttact tgactccttg gaactgggat gcaaatggta
     5401 agaaactttc tactgagaag gaaaagatgt actacttcaa tacgcaggcc ggtacaacaa
     5461 cttggaccct tccaagcgat tgggcaaaga gcaaggttta cctttacaag ctaactgacc
     5521 aaggtaagac agaagagcaa gaactaactg taaaagatgg taaaattacc ctagatcttc
     5581 tagcaaatca accatacgtt ctctatcgtt cgaaacaaac caatcctgaa atgtcatgga
     5641 gtgaaggcat gcacatctat gaccaaggat ttaatagcgg taccttgaaa cattggacca
     5701 tttcaggcga tgcttctaag gcagaaattg tcaagtctca aggggcaaac gatatgcttc
     5761 gtattcaagg aaacaaagaa aaagttagtc tcactcagaa attaactggc ttgaaaccaa
     5821 ataccaagta tgccgtttat gtcggtgtcg ataaccgtag taatgccaag gcgagcatca
     5881 ctgtaaacac tggtgaaaaa gaagtgacta cttataccaa taagtctctc gccctcaact
     5941 atgttaaggc ctacgcccac aatacacgtc gtaacaatgc tacagttgac gatacaagtt
     6001 acttccaaaa catgtacgcc ttctttacaa ctggatcgga cgtatcaaat gttactctga
     6061 cattgagtcg tgaagctggt gatgaagcaa cttactttga tgaaattcgt acctttgaaa
     6121 acaattcaag catgtacgga gacaagcatg atacaggtaa aggcaccttc aagcaagact
     6181 ttgaaaatgt tgctcagggt atcttcccat ttgtagtggg tggtgtcgaa ggtgtcgaag
     6241 acaaccgcac tcacttgtct gaaaaacacg atccatatac acaacgtggt tggaatggta
     6301 agaaagtcga tgatgttatc gaaggaaatt ggtcactcaa gacaaatgga ctagtgagtc
     6361 gtcgtaactt ggtttaccaa accatcccac aaaacttccg ctttgaagct ggtaagacct
     6421 accgtgtaac ctttgaatac gaagcaggtt cagacaatac ctatgctttt gtagtcggta
     6481 agggagaatt ccaatcaggt cgtcgtggta ctcaagcaag caacttggaa atgcatgaat
     6541 tgccaaatac ttggacagat tctaagaaag ccaagaaggt aaccttcctt gtgacaggtg
     6601 cagaaacagg ggatacttgg gtaggtatct actcaactgg caatgcaagt aatactcgta
     6661 gtgattctgg tggaaatgcc aacttccgtg gttataacga cttcatgatg gataatcttc
     6721 aaatcgaaga aattacccta acaggtaaga tgttgacaga aaatgctctg aagaactact
     6781 tgccaacggt tgccatgact aactacacca aagagtctat ggatgctttg aaagaggcgg
     6841 tctttaacct cagtcaggcc gatgatgata tcagtgtgga agaagcgcgt gcagagattg
     6901 ccaagattga agccttgaag aatgctttgg ttcagaagaa aacggctttg gtagcagatg
     6961 actttgcaag tcttacagct cctgctcagg ctcaagaagg tcttgcaaat gcctttgatg
     7021 gaaacttatc tagtttatgg catacatcat ggggcggagg agatgtaggc aagcctgcaa
     7081 ccatggtctt gaaagaacca actgaaataa ctggacttcg ttatgttcca cgtggatcag
     7141 gttcaaatgg taacttgcgt gatgtgaaac ttgttgtgac agatgagtct ggcaaggagc
     7201 atacctttac tgcaactgat tggccagata acaataagtc aaaagatatt gactttggta
     7261 agacaatcaa ggctaagaaa attgtcctta ctggtaccaa gacttacgga gatggtggcg
     7321 ataaatacca atctgcagcg gaactcatct ttactcgtcc acaggtagca gaaacacctc
     7381 ttgacttgtc aggctatgaa gcagctttgg ctaaggctca gaaattaaca gacaaagaca
     7441 atcaagagga agtagctagc gttcaggcaa gcatgaaata tgcgacggat aaccatctct
     7501 tgacggaaag aatggtggaa tactttgcag attatctcaa ccaattaaaa gattctgcta
     7561 cgaaaccaga tgctccaact gtagagaaac ctgagtttaa acttagctct ttagcttccg
     7621 atcaaggtaa gacgccagat tataagcaag aaatagctag accagaaaca cctgaacaaa
     7681 tcttgccagc aacaggtgag agtcaatctg acacagccct cttcctagca ggtgttagcc
     7741 tagccctatc tgctctcttt gtagtaaaaa cgaagaaaga ctagtattta gtaaaacctc
     7801 ttaacaagat tacggaagca gtctctatct tttccaatga ggtttatagt acagaaaaag
     7861 cctgagaaga tgtcttctca ggcttttgtt aagcacataa atacaatagt gctatgacaa
     7921 aatcacctag aaaaatctgg gtgataaatg ttatggttgt gctggttgag gattctgatt
     7981 ttgttgatca ggggttgtat ttgattgttg cgtattattg ttaggattgg tagtcgtact
     8041 attatttgtg cttggagtgg ttgagctaga ctgtgaagtt gaactatctg atgatgagct
     8101 tgaactttca gttgatgggg gttgttgtgg agcaggtgag ttccacgtag aacgagcacc
     8161 atttttaaat acgaattctc catttctgta gagcccctct ggtatattcc aatcttctgg
     8221 attgcttcct tcagacaggt aggtcatcat agagcggtaa actttggcag cgaccgtaag
     8281 gccattgcct acaagtggtg tcagacggtt agaatagcct gtccatacag ccattgaata
     8341 tttacgcgta tagccagcaa atagttcatc aggtgctaca aattgagagg tcttgatgtg
     8401 gttttcaatt tcctcgtctg tatagttaga ggttcctgtt ttaccagcct gaggaagcca
     8461 agcaagatag gcatttcgtc cagttccata agtcaagact gttttcatca tgtcggtcat
     8521 catataggct gtcgtttctt tcatggcacg agttccgaca ttagagaact ctttttcact
     8581 cccatcacta aagacgactt tatggatata cattggttta tagtaagttc caccatttgc
     8641 aaaggcagcg taagcagcag ccatcttttc actacttgct ccatattttt tgtctgattc
     8701 ggttgtgtta cttgaaatgg catttgagta gtgaatactt gggtagtcga ttccgagacc
     8761 atttaggaaa gtcttggcgc ggttgagtcc gaccttgttt agagtttcca cggctgggac
     8821 gtttcgcgat tgttgcaggg cgtattgcaa ggtgatgttg ccaaagtagc ccctatccca
     8881 gttataaaca ggagtatttg tcccagggta attatagggc tcatcgtgaa cgatagtagc
     8941 agttgaatcg tagacaccgt actccaaggc aggagcatag tctgtgatcg gtttcatagt
     9001 tgatccccag tcgcggtttg tttctactgc ttggttaatt ccgaaggaaa cattacttga
     9061 ctgatggcgt gctcctagct gggcaatgac tttaccgtta gaaacatcaa caatggtaga
     9121 agcgacttgc aattcatcgt ctggataggc aacgtattcg tctgtattgt aaatatccca
     9181 cagatgtttt tgagcttctt ggtctacatt tgtgtagaca tccatcccag ttgtgagcag
     9241 gttatagcct gtttcttctt caacttgatt gatgacttcc ttgaggtaat tatccatgta
     9301 agcagggtaa ttacttgctg atttgagact ttgtagtcca tcagtaattg gtgtattgac
     9361 tgctttctca tactgttcag cagagatgta gccttgattt ttcatttcag ataagaccaa
     9421 gtttcggcgg tcttgggctg cttctggatg tgaatagggg tcatattggt ttggtgcctg
     9481 aggcattcca gccagcaagg ctaactgagg taaacttaaa ttattgaggt ctttaccata
     9541 gtagttttga gctgctgtct gcattccata gttcccatta gacatgtaga ccttatttat
     9601 atagtaggtc aagatttctt gcttggttgc tttttgttct aactgaatcg ctaaccaagc
     9661 ttcctgagcc ttacgagaaa tagtctggtc ggaagtcgaa gttgaaaagt aagtcaactt
     9721 aatcaactgt tgggtgagag ttgatccacc ttggagggaa ttgctttgca gattgcgcaa
     9781 gaaagctccc aggatacgga tggtatcaat ccccctgtgg tcgaagaagc gatggtcttc
     9841 gatagaaacg attgccttaa ccaaatctgt gggaatatca ttagcttggg cattgacgcg
     9901 gcgttcagaa cccaagtcag caatgagttg atttttattg tcgtagattt tactagaagt
     9961 tgttgcaact agtttactct cggataggct aggagccttg ctaacgtagt agaaaaaaac
    10021 tcctccgcct aagacaatgg ctgcgataac caagcttaag aagctaatgc tcagatactt
    10081 gattaggcgc agaatcgttg gtttgttcat cttgttttac cacctaataa atgttctttg
    10141 ataacattga gataaggaat ttgagggaag gcaccagcct tgatttcata tccatattct
    10201 cgaatatatt caagtggcat tgatttttgt cccttatctt gatgatagaa gcgaatcaaa
    10261 tcgaatgccg gcaataagta ggtttcttgc tgagaagaaa agtgaagaag gacaaagcag
    10321 attccttgtt gggcaaggac ttgttccata tgctgaatct gatgtggatg aaaatttttc
    10381 atcggaatcg cacgtttttg ttttgtttcc ttgacttcaa agtcgatgta atatccatta
    10441 taaacgccag aatagtccgt cgttgaagct tgtcgaaaat aggcttcaac aatcttggca
    10501 cgacttcgtt gtggatagtc cacttgtacg atttgaatag gagttggttt cttatgtata
    10561 acagccaagc cctgagacaa atagtagtcg ttggtagcat tgatcatctt ttcaaaagac
    10621 attcctcgat ttgcgaaatt tttgggttga gaaagagatg tttgtctttt ttgtgatgaa
    10681 actttatgtg gatagttgac cataattctc cttattggta caataacatc actttattat
    10741 accataattt gcacagaaag ggttaaaaat ggctacagct ttggttttag gctattctgc
    10801 ttttgatttg ggtttatttt ctgataagga tcctcgtctt aaattgatta aaaaagcgat
    10861 acgtaaagat ttagaggcta tggcagcaga tggggtgtct tggcttgtgt ttacaggaag
    10921 tttgggtttt gaatattggg ttttagaggt tgctcaggag atgaaaaccg aatacggttt
    10981 ccagttggcc accatttttg cttttgaaac ccatggggaa aattggaatg aaggcaatca
    11041 gatgaaactg agtcgcttta agcaggtaga ctttgtcaaa tatgcttatc ctcgctatga
    11101 acacaagggg cagttaagag attaccagca gtttttgctg gaaaacacga ctagttccta
    11161 tcttttttat gatgaagaaa atgaaacaaa actagcttat ttttaccaaa agatgaaaaa
    11221 tcaagaggac tattttataa agagattaac atttgatcaa ttaaatgaac ttgctgaaaa
    11281 tttttccgaa aattgaagct ttgaccttga tttttgtttg ccttttttta tataataata
    11341 ctagcaagcg agaatggaga gagacatggc aagtattatt ttttcagcga aagatatttt
    11401 tgaacaagag tttggacgtg aagtccgtgg ctataataaa gtagaagttg acgagttttt
    11461 agacgatgtc atcaaggact atgaaaccta tgctgccttg gtcaagtcac ttcgtcagga
    11521 aattgcggat ttgaaggaag aattaactcg taaaccgaaa ccttcaccag ttcaagcaga
    11581 accccttgaa gcggcaatta caagttctat gacgaatttt gatattttga aacgcctgaa
    11641 tagattggaa aaagaagttt ttggtaaaca aattttagat aactcagatt tttaagtagt
    11701 tatttgagat gtgcaatttt tggataatcg cgtgaggaga attgcttctc atgaggaaag
    11761 tccatgctag cacaggctgt gatgcctgta gtgtttgtgc taggcgaaac cataagccta
    11821 gggacgagga atcgttacgg cagttgaaat ggctaagtcc ttggataggc cagagtaggc
    11881 ttgaaagtgc cacagtgacg aagtctttct ggaaacagag agagtggaac gcggtaaacc
    11941 cctcaagcta gcaacccaaa ttttggtcgg ggcatggagt acgcggaaac gaacgtagta
    12001 ttctgactgc tatcagctag agctgttagt ggtagacaga tgattatcga aggaagtggt
    12061 cctagtcact tctggaacaa aacatggctt atagaaaatt gcatataggt tggggctgag
    12121 aaattttctc aacctcattt tttaaagtgg acatatagaa aggtcttgca agactgtaac
    12181 atgaaaaaag aatttaattt aattgcaact gcggcagcag ggcttgaggc tgtcgttggt
    12241 cgtgaagtgc gagagttggg ctacgattgt cagattgaaa atggacgtgt tcgttttcaa
    12301 ggagacgtga gagctattat cgaaaccaac ctttggctgc gggcagcaga tcgtatcaaa
    12361 attatcgtag gaacgttccc tgctaagact tttgaagagc tatttcaggg agttttcgct
    12421 ttagattggg aaaattattt accacttgga gctcggttcc cgatttcaaa agctaaatgt
    12481 gttaagtcca aacttcacaa tgagcccagt gttcaggcta tttctaagaa agctgttgtc
    12541 aagaaattgc agaaacacta tgctcgccca gaaggggttc ctctgatgga gaatggccca
    12601 gagtttaaga ttgaggtctc tattctcaaa gatgtggcaa ctgtcatgat tgatacgacc
    12661 gggtctagcc tctttaaacg tggttatcgt accgaaaaag gtggcgctcc tatcaaggaa
    12721 aatatggcag cagccatttt acaactttct aactggtatc cagacaagcc tttgattgat
    12781 ccgacctgtg gttcggggac tttctgtatt gaggcagtta tgattgctag aaagatggcg
    12841 ccaggtcttc gtcgctcttt tgcatttgag gaatggaact ggatcagcga tcgcttgatt
    12901 caagaagtgc gcacagaagc ggctaaaaaa gtagaccgtg agcttgagct ggatatcatg
    12961 ggctgtgata ttgatgctcg catggtggaa attgctaagg ccaatgctca ggcagctggt
    13021 gttgcaggag acattacttt taagcagatg cgcgtgcagg atttacgttc cgataaaatc
    13081 aatggagtaa tcatttccaa tccgccttat ggtgaacgtt tgtcagatga tgcaggggtg
    13141 accaagctct atgctgagat ggggcaagta tttgcaccgc tgaaaacttg gagcaaattt
    13201 atcctgacta gtgatgaagc ttttgaaagc aagtatggta gccaagcaga taagaagcgt
    13261 aagttataca acggaacctt gaaagtggat ctgtatcaat attttggtca gcgtgtcaaa
    13321 cggcaagagg taaaatagaa agggatactc atgagtaaaa aaagacgaaa tcgtcaaaaa
    13381 aaagaaggtc aagaaccgca atttgatttt gatgaagcaa aagagctaac agttggtcaa
    13441 gctattcgta aaaatgaaga agtggaagca ggagtcttgc ctgaggattc cattttggac
    13501 aagtatgtta agcaacacag agatgaaatt gaggcggata agtttgcgac tcgtcaatac
    13561 aaaaaagagg agttcgttga aactcagagt ctggatgatt taattcaaga gatgcgtgag
    13621 gctgtagaga agtcagaagc ttcttcggag gaagttccat cttctgaaga catcttacta
    13681 cccttgcctc tggacgatga ggagcaaggc ttggatcctc tattgctaga tgatgaaaat
    13741 ccaacagaaa tgactgaaga agtggaagag gagcaaaacc tttctcgtct ggatcaagag
    13801 gactcagaaa agaaaagtaa aaaaggcttt attttgaccg ttttggcgct tgtatcagta
    13861 attatttgtg tcagtgctta ttatgtctac cgtcaagtgg ctcgttcgac caaggaaatt
    13921 gaaacttctc aatcaactac agccaatcaa tcggatgtgg atgattttaa tacactttat
    13981 gacgcctttt acacagatag caataaaacg gctttgaaaa atagccagtt tgataaactg
    14041 aatcaactca agactttact tgataagctg gaaggtagtc gtgaacatac gcttgccaaa
    14101 tctaaatatg atagtctagt aacgcaaatc aaggctattc aagatgtcaa tgctcaattt
    14161 gagaaaccag ctattgtgga tggtgtgttg gatgccaatg ccaaagccaa atcggatgct
    14221 aaatttacgg atattaaaac tggaaatacg gagcttgata aagtgctaga taaggctatc
    14281 agtcttggta agagccagca aacaagtact tctagctcaa gttcaagtca agcaagctca
    14341 aatacgacaa gtgagccaaa accaagtagt tcaaatgaga ctagaagtag tcgcagtgaa
    14401 gtcaatatgg gtctctcgag tgaaggggtt gctgttcaaa gaagtgccag tcgtgttgcc
    14461 tataatcagt ctgctattga tgatagtaat aactctgcct gggattttgc ggatggtgtc
    14521 ttggaacaaa ttctagcgac ttcacgttca cgtggctata tcactggaga ccaatatatc
    14581 cttgaacgtg tcaatatcgt taacggcaat ggttattaca acctctataa gccagatgga
    14641 acctatctct ttacccttaa ctgtaagaca ggctactttg tcggaaatgg cgctggtcat
    14701 gcagatgact tggactacta agcagtcgtt acaaaattct ttcttttcaa aagtaaaaat
    14761 gataaaataa aacaaatgaa acaagaggag tgtcaaatga caaaagctaa ctttggtgtc
    14821 gtaggtatgg ccgtaatggg tcgtaacctt gcccttaata ttgaatcacg tggttacaca
    14881 gttgctatct acaaccgtag taaagaaaaa acggaagatg tgattgcttg ccatcctgaa
    14941 aagaattttg taccaagcta tgacgttgaa agttttgtaa actcaatcga aaaacctcgt
    15001 cgtatcatgc tgatggttca agctggacct ggtacagatg ctactatcca agcccttctt
    15061 ccacaccttg acaagggtga tatcttgatt gacggaggaa acactttcta caaagatacc
    15121 atccgtcgta atgaagaatt ggcaaactca ggtatcaact ttatcggtac tggagtttct
    15181 ggtggtgaaa aaggtgccct tgaaggtcct tctatcatgc ctggtggaca aaaagaggcc
    15241 tacgaattgg tttcggatgt tcttgaagaa atctcagcta aagcaccaga agatggcaaa
    15301 ccatgtgtga cttatatcgg tcctgatgga gctggtcact atgtgaaaat ggttcacaac
    15361 ggtatcgagt acggtgatat gcaattgatc gcagaaagct atgacttgat gcaacacttg
    15421 ctaggccttt ctgcagagga tatggctgaa atctttactg agtggaacaa gggtgaattg
    15481 gatagctact tgatcgaaat cacagctgat atcttgagcc gtaaagacga tgaagaccaa
    15541 gatggaccaa tcgtagacta catccttgat gctgcaggta acaagggaac tggtaaatgg
    15601 acgagccaat catctcttga ccttggtgta ccattgtcac tgattactga gtcagtgttt
    15661 gcacgctaca tttcaactta caaagaagaa cgtgtacatg ctagcaaggt gcttccaaaa
    15721 ccagctgcct tcaactttga aggagacaag gctgaattga ttgaaaaaat ccgtcaagcc
    15781 ctttacttct caaaaatcat ttcatacgca caaggatttg ctcaattgcg tgtagcctct
    15841 aaagaaaaca actggaactt gccatttgca gatatcgcat ctatctggcg tgatggctgt
    15901 atcatccgtt ctcgtttctt gcaaaagatt acagatgctt acaaccgcga tgcagatctt
    15961 gccaaccttc ttttggacga gtacttcttg gatgttactg ctaagtatca acaagcagtg
    16021 cgtgatatcg tagctcttgc ggttcaagct ggtgtgccag tgccaacttt ctcagcagct
    16081 attacttact ttgatagcta ccgttcagct gaccttccgg ctaacttgat ccaagcacaa
    16141 cgtgactact ttggtgctca cacttaccaa cgtaaagaca aagaaggaac cttccactac
    16201 tcttggtatg acgaaaaata agtaggtcag ccatggggaa acggatttta ttacttgaga
    16261 aagaacgaaa tctagctcat tttttaagtt tggaactcca gaaagagcag tatcgggttg
    16321 atctggtaga ggaggggcaa aaagcccttt ccatggctct tcagacagac tatgatttga
    16381 ttttattgaa cgttaatctg ggagatatga tggctcagga ttttgcagaa aaattgagtc
    16441 gaactaaacc tgcctcagtc atcatgattt tagatcattg ggaagacttg caagaagagc
    16501 tggaagttgt tcagcgtttt gcagtttcat acatctataa gccagtcttt atcgaaaatc
    16561 tggtagcgcg tatttcggcg atcttccgag gtcgggactt cattgatcaa cactgcagtc
    16621 tgatgaaagt tccaaggacc taccgcaatc ttaggataga tgttgaacat cacacggttt
    16681 atcgtggtga agagatgatt gctctgaccc gccgtgagta tgaccttttg gcgacactta
    16741 tgggaagcaa gaaagtattg actcgtgagc aactgttgga aagtgtttgg aagtatgaaa
    16801 gtgcgaccga gacaaatatc gtagatgtct atatccgcta tctacggagc aagcttgatg
    16861 ttaaaggtca aaaaagctac attaaaaccg tgcgtggtgt tggttacacc atgcaagaat
    16921 agaaaagcag ttgcagttgt gtaactgctt ttttgaggag tttttatata ttgacatgca
    16981 gtcgggtctt tgctacaatc gttttaggag gatatataga tgaaactttt gaaaaaaatg
    17041 atgcaagttc tactagcagt ctttttcttt ggtttgctag ctacaaatac ggtatttgcg
    17101 aataccacag gtggccgatt tgttgataag gataatagaa aatattatgt aaaagatgat
    17161 cataaagcaa tctattggca taaaatagac ggtaaaactt actattttgg tgatattgga
    17221 gagatggttg tcggttggca atacttagaa attcctggaa caggttatcg tgataattta
    17281 ttcgataacc aaccagttaa tgaaattggc cttcaggaga agtggtacta ttttggacaa
    17341 gatggtgctt tgctagaaca aacagataaa caagtactag aggcaaaaac gtctgaaaat
    17401 acaggaaaag tatacggtga acaatatcct ctatctgctg aaaagagaac ttattatttt
    17461 gataataatt atgctgtaaa gacaggctgg atttatgaag acggcaattg gtattattta
    17521 aataagctag gaaattttgg cgatgattct tacaatccac taccaattgg tgaagttgct
    17581 aagggttgga ctcaagattt tcatgttact attgacattg atagaagcaa acttgctcca
    17641 tggtactacc tagacccaga aactggcatt atgcgaacag gatggcaata tctaggtaat
    17701 aactggtact acctccgttc atcaggagca atggcaacag gttggaaata tgtacgaggc
    17761 aaatggtatt acttagataa taaaaatggt gatatgaaga ctggttggta tcaagatggt
    17821 tcaacttggt actatttaga tccttctaat ggagatatga aaataggttg gacaaaagta
    17881 aatggaaaat ggtattatct caattcaaat ggagcaatgg ttacaggtag tcaaactatc
    17941 gatggtaaag tttataactt cgcttcatct ggtgagtgga tttaatgttg gaggatatat
    18001 aaaatgaagc ttttgaaaaa aactatgcaa gctggactaa cagtaatttt ctttggtttg
    18061 ctagctacga atacagtatt tgcagataac tctgaaggat ggcagtttgt ccaagaaaac
    18121 ggtagaacct attacaaaaa gggggacctc aaagaaacct actggcgagt gattgatggt
    18181 aagtactatt attttgattc tctatctgga gagatggttg tcggctggca atatatcccg
    18241 tttccatcta aaggtagtac aattggtcct tacccaaatg gtatcagatt agaaggtttt
    18301 ccaaagtcag agtggtacta ctttgataaa aatggagtgc tacaagagtt tgttggttgg
    18361 aaagaattag agattaaaac tcaagacagt gttggaagaa agtatgggga aaaacgtgaa
    18421 aattcagaag ataaagaaga gaagcgttat tacacgaact attactttaa tcaaaatcat
    18481 tctttagaga caggttggct ttatgatcag tctaactggt attatctagc taaaacagaa
    18541 attaatggag aaaactatat tggtggtgaa agacgtgcag gttggataaa tgatgcttca
    18601 gcttggtact acctagatcc agcaactggt attatgcaaa caggttggca atatctaggt
    18661 aataagtggt actacctccg ttcctcagga gcaatggcca ctggctggta tcaggaaggt
    18721 accacttggt attatttaga ccacccaaat ggcgatatga aaacaggttg gcaaaacctt
    18781 gggaacaaat ggtactatct ccgttcctca ggagctatgg caactggttg gtatcaagat
    18841 ggttcaactt ggtactacct aaatgcaggt aatggagaca tgaagacagg ttggttccag
    18901 gtcaatggca actggtacta tgcttatagc tcaggtgctt tggcagtgaa tatgaccgta
    18961 gatggctatt ctgtcaacta taatggcgaa tgggttcggt aatgacaggg cgcttataat
    19021 tatattagaa cactcgctta atcaaagaag tgactttgtt gtacttgacc tgacgattgg
    19081 ggagattaaa agcatgtgaa atctacacat gctttttcgt tagttatgag tgttgaactt
    19141 gactaaccat tgacaaaaga taagagcaat caaaataaga tacaaaaata tcaatgctaa
    19201 ctgcaccgaa acgcccgatg ctatggatag ggctaatagg gagagcaggg acggggcgag
    19261 aatgtctact gtgctcagag cagagcccac cgtccccatc ttgtcactgg ggatatgggc
    19321 aaagacagct gcttgtaggc gagggctgag aatgccgata atcgtagaac tgatgaaact
    19381 ggtcacgaaa attacgatat aggcttgaca aaggatacta gttattagag ccaataggga
    19441 aatctcacag aaaaccaaga ggtgcgtgaa acggatattt ttaaatagat tactgctggt
    19501 catattgcca aggatagctc cacaagacat gatgatggag agcagggaga agagttgccc
    19561 aatgctcagt tgtgctagtg tctgttggat gaagcggagg gcaatcagcc gaggggacac
    19621 atccaagatg gcaatcatac tggtcgtcag aaacagtagt atgacggtat cttttaacct
    19681 caggagcaca gccagcgact gtttaagatg ttgaaaatat ttcttaaaac tcagtgctgt
    19741 atgtgacatt tcaatccttt tttcaacctc atacaaggta tgtcgaataa catacaggcc
    19801 caaaaaggca atgacaaaag tcagagtgtt gataagggaa atagtttgaa tactcataac
    19861 attgataagg aatgcgccag ctagattggc gacaatgcgg acaaccctca tcagagactg
    19921 cctgaacccc atggcatcat gcaggtcgtc cttaattaca ctgatgtaga gggcgttcat
    19981 catgtaggca gacaggtagc tcaaggtgtc agagatgagg ttgatgatga caatcataat
    20041 gactgaaact aatgaaaagg gcgacaagac gagaaatact gtcaaaatag cgtagagcag
    20101 gattttgatt ttggcaatcc agagtgcgtg tttgactcta ttttcttgaa aatctcctag
    20161 aattccgaga aagagttgcg atagtagggg aaccacctct gaaatggaaa tcagtaaaat
    20221 cgctagcgga gcgaaattgt actgagccac ataattgata atggcaaggt agaaaatact
    20281 gtcgccgaat gatgaaaaac agctgtttag caacaactgt ctatatatgt tgtttgtcca
    20341 aaataatttc atacatttct ccttatgtct gcacatcgaa tgggaattta ttatttaata
    20401 gacaccaata tacccccatg cttcctattc cgaagtcaaa caaatctgct ttaccactat
    20461 ctccttgaaa catcagtaaa ccgtgatcat ctcgttcaga acatgctaaa attacctgtt
    20521 ggattttttt catcagtaat ttgttttgat tgtagacagt ggtttgtagt aagctagtta
    20581 ttccgtggca atatcctgtc atcattttta gatgatggtt aaaaacaaat tcttgatatt
    20641 tactaataat atctttgttt ccgtcacaat cgtacataca aagatataag attattccgg
    20701 aaattccttt gcaccaagaa agttgtaaat ttcctaaatt ttcagtatta gaagtaactt
    20761 tttctaataa tttttttaat tcagtatgga atgtatggag atcattatag aacatagaag
    20821 gttctaagac tttagaatag gcaaataaag cctatgcaat tccacaatat ccatgagcaa
    20881 agctatagtc taaaatgctt tctttggcta tatctctttg taggaaataa tgctaaattt
    20941 cccctatact attgtggatg aaaattttaa gagtattgtc attggtaaat tgatagtatt
    21001 ttattaatga taataggaca ccagattttc ccaatgcaaa gtctatttcc tctagagtat
    21061 cataattctc tattaatttc ttagcggtga cgtttgctaa ttgtagatag tattgatttt
    21121 tactgataga aaataaatgt aggagtaacc atagataacc tgcatcacca aatagcagtg
    21181 agtattgata ttcatatgtg tgtgtcgttc taattgtcac tgccttcttg atgatagaga
    21241 gtattttaga atccagttct gggtggtaca atttatttag aggaaacagt agtccagcta
    21301 ttccgtgttg gaatgataaa ttacttacga aatttccaaa ttctgttgaa gaagtgatcc
    21361 ttccttcaga atttacagtt ttatctagta aaaatcgtag aacactgtta cattctgttt
    21421 tgtaatcata tttatgttcc tctatttttt taattttgac taaattattt gtttgactta
    21481 aatttagtag acattgtaac caaaatagtt gctcatctgt gagctgacct tgagtatgtg
    21541 atagttctag taattgaata ggatccttta gttagattca atcaatctat aacacaacaa
    21601 cataataata cttttaggat aacttatcaa atattatagt aaaatgagat aataacagga
    21661 taaatcga
//
LOCUS       DBEROU010000039        21629 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.039, whole genome shotgun sequence.
ACCESSION   DBEROU010000039 DBEROU010000000
VERSION     DBEROU010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.039"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..1487)
                     /gene="pcpA"
                     /locus_tag="ACL8CL_001599"
     CDS             complement(<1..1487)
                     /gene="pcpA"
                     /locus_tag="ACL8CL_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HGS1330821.1"
                     /translation="MKKTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSII
                     QQNNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVA
                     SQAFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRA
                     FDFSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLT
                     LPKSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESY
                     KTPKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERL
                     AFYGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEI
                     HIKNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETD
                     VAKPKKNSNQGVVGWVKDKGLWYYLNESGSMATGWVKDKGLWYYL"
     gene            1822..1965
                     /locus_tag="ACL8CL_001600"
     CDS             1822..1965
                     /locus_tag="ACL8CL_001600"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1330822.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KKH"
     gene            1949..2804
                     /locus_tag="ACL8CL_001601"
     CDS             join(1949..2368,2370..2804)
                     /locus_tag="ACL8CL_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HGS1330823.1"
                     /translation="MKKNIDPYQGVNRLPAKQNYEASKQLTDARFKRLVGVQRTTFEE
                     MLAVLKTAYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIATDFGIHESNLIRR
                     SQWVEVTLVQSGVTISRTPLSSEDTVMIDATEVQINRPKKELANDSGKKKCHAMKAQA
                     IVTSQGRIVSLDITVNYCHDMKLFKMSCRNIGQAGKILADSGYQGLMKICPQAQTSRK
                     SSKLKPLTAEDKAYNHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAG
                     IINHELGF"
     gene            complement(2883..3140)
                     /locus_tag="ACL8CL_001602"
     CDS             complement(2883..3140)
                     /locus_tag="ACL8CL_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330824.1"
                     /translation="MLPTYTDSYFLSFVSDDVEERYTENVYLDSLSVKQKFKFIFDFG
                     DEWRFECQVLREIETEDEEAYLVRSVGTSPEQYPDYDGFDY"
     gene            complement(3499..5379)
                     /locus_tag="ACL8CL_001603"
     CDS             complement(3499..5379)
                     /locus_tag="ACL8CL_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HGS1330825.1"
                     /translation="MVRFTGLSSKQAQAIEVLKNHISLPDVEVAVTQSDQASISIKGE
                     GGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVEDILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEVYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(5373..6242)
                     /locus_tag="ACL8CL_001604"
     CDS             complement(5373..6242)
                     /locus_tag="ACL8CL_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HGS1330826.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILNKTSIPTPESLEDLLAWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQIPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGQTDWDGRKIYQEAAAGNAICQEAIERMNRNLAQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDTYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEENQW"
     gene            complement(6326..8971)
                     /locus_tag="ACL8CL_001605"
     CDS             complement(6326..8971)
                     /locus_tag="ACL8CL_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HGS1330827.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVAAFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTILVHLAPLSWTTFQLLEGKQE
                     HREGIYQNGVIDTPFVTVSVDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTDRYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTELIKKEEI"
     gene            complement(9062..10342)
                     /locus_tag="ACL8CL_001606"
     CDS             complement(9062..10342)
                     /locus_tag="ACL8CL_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HGS1330828.1"
                     /translation="MIYSKEIVREWLDEVAERAKDHPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDETF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            10513..12597
                     /locus_tag="ACL8CL_001607"
     CDS             10513..12597
                     /locus_tag="ACL8CL_001607"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HGS1330829.1"
                     /translation="MKPLLETIDTRFGTASKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QGGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDIDK
                     ACFQPHYLKLFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHAADELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEGGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(12637..14316)
                     /locus_tag="ACL8CL_001608"
     CDS             complement(12637..14316)
                     /locus_tag="ACL8CL_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HGS1330830.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYDQEWGTGQE
                     DPERFNPSQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNPDKLGTEAELNYLQYGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWANDADLPIQLELDLGSPKTFDVIELREDLKLGQRIS
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSMPLLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIQVLQFQAGESKKDLHLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            15072..16301
                     /gene="arcA"
                     /locus_tag="ACL8CL_001609"
     CDS             15072..16301
                     /gene="arcA"
                     /locus_tag="ACL8CL_001609"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HGS1330831.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLGVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            16359..17375
                     /gene="argF"
                     /locus_tag="ACL8CL_001610"
     CDS             16359..17375
                     /gene="argF"
                     /locus_tag="ACL8CL_001610"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HGS1330832.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            17540..18487
                     /gene="arcC"
                     /locus_tag="ACL8CL_001611"
     CDS             17540..18487
                     /gene="arcC"
                     /locus_tag="ACL8CL_001611"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HGS1330833.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            18698..20209
                     /locus_tag="ACL8CL_001612"
     CDS             18698..20209
                     /locus_tag="ACL8CL_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HGS1330834.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNTWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            20231..21562
                     /locus_tag="ACL8CL_001613"
     CDS             20231..21562
                     /locus_tag="ACL8CL_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HGS1330835.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICRDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
BASE COUNT         6223 a         4746 c         4324 g         6336 t
ORIGIN      
        1 tttaagtaat accataaacc tttgtcttta acccaaccag tagccattga acctgattcg
       61 tttaagtaat accataaacc tttgtcttta acccaaccaa ctactccctg attagaattc
      121 tttttaggtt tagctacatc agtttcttgt tttatattat ctactttttc aacaatgata
      181 tcattactag tagataaatt tgatttaaga acatctttta tatgttctga tgttacatag
      241 aacttaacag tttcaggaat tgcaaatgta tcttttttca cagaaaactc tgtactttta
      301 ttcttaatat gaatttcctt taatgaatct aagacgccac ttaggaagaa ggacggcaat
      361 gagttgatat tattaccaat tgttaaactt tttaattttg gtaaaccgtt cataacgtgt
      421 ttaccaaaat ttttaacatt atctggtaat ataagttctt ttaattctaa attaccgtaa
      481 aaggctaaac gttcaatagt ttctaaacta tttggtaagc taatttcttc aagtttaatc
      541 gcatctgcaa atgcaaaagt accgattttt tctaaacctt cattcaattc gagttttttc
      601 aagtaagaat ttttattaaa cgaatatgat gcaagttctt ttgtctcctt aggcgtttta
      661 taactttcgt catttttttg acttggataa taaattaatt gggttttatc ttttgaaaac
      721 aaaacaccat caactgaggc aaacgattca tttccttctt caacatcaac atgttttaag
      781 ctagtagtga gtctaaatag attacttcct aatgttttaa ccgattttgg taatgttagt
      841 ttctctaaat ttgataaatt agcaaaagcc tcatgtgata ttaattctaa ttttgaactt
      901 gaggaaaagg ttagcttttt caatttttga ctaaaagaaa atgcacttgt tccaatatat
      961 tctagagatg caggcaattc aatctcttct aattcagaaa aatcaaaagc tctatcttca
     1021 attttctcca gctgactgcc tttgtcaaaa ataatagttt tagctttact tccatggaag
     1081 cctttttttt gtattttttt tatgcttgat gggatagtaa tagagctagg atagtagaca
     1141 attctacctg tttcatctgg ataataacta aaagcttgac tagctacttc agtaacggta
     1201 aatactttat cacctactgt gatttttgta ggaatcgtga atggttttcc atcttgacca
     1261 gcttcaaact ctacaggagt aactccatta cccacttgaa cagttcctat gttaccttct
     1321 acagttagaa ccttatattt gatattattt tgttgaataa tacttccaac tttagtctct
     1381 ttgattactt ccgaactagg agtatctgct aggattggtt cattagggac atatgctgct
     1441 aaaataaccg cagctgtagt taatgataat attgtagttt ttttcattta aatttcctta
     1501 catatttatt ttctaataga tataaaatta taactttgct agccgttgtt ataaaaagtt
     1561 ttattcaatc attatctagt agaatagagt agtacatttt ataaattgtc atctctctat
     1621 aaacacagta tatcattttt aaattttaag tcaaggaaaa ttaacccact ttaatttctt
     1681 gtttaacccg ggttaaaaaa gaaattaaag tgggttaaaa aagaaattaa ctgagtaaaa
     1741 actctcgaaa ttcaattcgc tatattatca ctcaacttga acaaatacca gaaccacaaa
     1801 aaacagatgt aaaggaaaaa tatggactta aaatttgaag gggttgattt agaatacaaa
     1861 aaagctaaaa acaatcttcc agagtctttc tgggaaacct actcagcatt tgcaaataca
     1921 aatggcggga aaataattct cgggatagat gaaaaaaaac attgacccct accaaggagt
     1981 caatagactt cctgcgaaac aaaattatga agcaagtaaa caactaaccg atgcacgatt
     2041 taagcgtctt gttggtgttc agcgcaccac ttttgaagag atgttagctg tgttaaaaac
     2101 agcttatcaa cttaaacacg caaaaggtgg acgaaaacct aaattaagcc tagaagacct
     2161 tcttatggcc actcttcaat atgtgcgaga ataccgcact tatgaagaaa ttgcgactga
     2221 ttttggtatt cacgaaagca acttaatccg tcggagccaa tgggttgaag taactcttgt
     2281 tcaaagtggt gttacgattt caagaactcc tctcagttct gaggacacgg taatgattga
     2341 tgcgacggaa gtacaaatca atcgccctaa aaaaagaatt agcgaatgat tctggtaaaa
     2401 agaaatgcca cgctatgaag gctcaagcga ttgtcacaag tcaagggaga attgtttctt
     2461 tggatatcac tgtgaactat tgtcatgata tgaagttgtt caaaatgagt tgcagaaata
     2521 tcggacaagc tggtaaaatc ttggctgaca gtggttatca agggctcatg aagatatgtc
     2581 ctcaagcaca aacttcacgt aaatccagca aactcaagcc actaacagct gaagataaag
     2641 cctataacca tgcgctatct aaggagagaa gcaaggttga gaacatcttt gccaaagtaa
     2701 aaacgtttaa aatgttttca acaacctatc gaaatcatcg taaacgcttc ggattacgaa
     2761 tgaatttgat tgctggtatt atcaatcatg aactaggatt ctagttttgc aggaagtcta
     2821 ttgaaatact aagcctacac agactgattc caattgtttt ttagatttca attttaccat
     2881 tcttaatagt caaaaccatc ataatctgga tattgttctg gcgacgttcc aaccgaacgt
     2941 acgagataag cttcttcgtc ctctgtctcg atttctctca gcacttggca ttcaaaacgc
     3001 cattcatcac cgaagtcgaa aataaactta aatttttgtt tgacacttag gctatccaga
     3061 tagacatttt ctgtgtaacg ttcttcaaca tcgtcactaa caaagctaag aaagtaagaa
     3121 tctgtatatg tcggtagcaa ccttttttaa atttgacgga aatgacccag gcttctacag
     3181 atgctgactt ttcagcttct atttcttatt ttactaattt taagtgtacc ataatacctc
     3241 caagtgctca tcgatagaat tgctcttagg aaacattcac tttctgctat tttatcataa
     3301 gtttgtctaa ctgtagcatt tgaaaccaga taattcttct gtttcacgcg aaacaccctg
     3361 cgccaagcaa ggtgttattg tttttatact caatgaaaat caaagagcaa actaggaagc
     3421 tagctgcagg ctgctcaaaa cactgttttg aggttgtaga tggaagctga cgtggtttga
     3481 agagattttc gaagagtatt aagtcgtata aatcgtcgaa gctgtcgcaa tggtatgcca
     3541 ctggttagct gtagttgctg cgaaatcctt gtctgcgtag aagtcagtaa atggtaggat
     3601 ttcaacttcc agctcgtcga tgcggtcaag ctggccagcc agataaacct cgatacggct
     3661 ttctgctcgt ttgatgcgtt gcaagagtcc gcccatacgg atgtcaactg tatccaaacc
     3721 aaagaccttg ttttctttca gccattggtg gctaaagagg gcatggaagt cttcaatttg
     3781 gcttctaagt tctggtaatt cttgtctggc gatttgttgt aaactttctt tatcatccgc
     3841 ttggtaggcc tgacgaatgc gtcgtcccac atctactttg ctacttaaaa tagcattcaa
     3901 ctgggcctga gtttcaaaga gataagcata gtttccagct ttttctttaa tgttagcaag
     3961 cgtctcagca gcctgagcga agtgcggttt gtcctgttca ggtgtcatgt gttgatcaag
     4021 aatcggacaa agaatatcct gataaaaaac atagcggttg ggattgatac cgctgagatt
     4081 gcctggtagg tctggtaaga ggttggcaag gtcaatctgc ataaaatcct caaccgttag
     4141 accagtattg gtcttgaagt gcgcagacaa accatctagg tcattgcgat agctgagttc
     4201 tgcccagatt tgcaagcttg gtaggataga gaactgggca gtttcaccac cattgtctcc
     4261 ccaaccagtt acgatgactt ctttaatctg attggcacgg caggctttat tagcctcgat
     4321 agccactaga cggctaaaat gattgtgagg tgtaaagcca atccacttcc aagctccccc
     4381 tgcaaatgca aggtcatggc taatcttgtg atgattgcgg aaattacggt tgtatttttc
     4441 ctcgctatcc tgataataat cccagtaaac cagagtcaca cggtctttga gacggtctag
     4501 gtagacacga gtttcctctg gaatttccac atcacggtcg tactggccat ccgctgacat
     4561 gagtttgaag aacatatcac tccacatctg gcagtggaaa ccatatttgt cagcaatatc
     4621 cagcacgcgc tccaagtgtt ggcacatgag gagactacga tccacaacac cgttcagaat
     4681 caggtagcgt cccaaaccaa ccaagtgggc ttcgtccatc ccgatattga ccttgcgagt
     4741 cttcagttta gacaacgtgg caaacatgcc atcaatcagg tcataaacct tttcttcgcc
     4801 aatgagaaga atgtcctcta catcacggag ctcctgcact tccttgacac cccatttgac
     4861 aaaggctgac aggtgggcca aggtctggat gcatggtaca aaggtcatgt caaactgttg
     4921 agcataggct tcgatttcct gcaactcctc tgctgaataa gctccacgga aatagccaaa
     4981 gtaaggctgc ccttcaatct ggtaagtgtc ttccatgtaa agctcaaagg ttgagtagcc
     5041 catgagagcc aagacctcaa tcatctgctt ggcagaagcc acattcagca ccgcatttcg
     5101 agaacagtca accatgtaag ccaaatcttc gtaagctgct tgttcctcaa tctctacttt
     5161 atcagcttct gctagaactg ttaccaacaa ggacaaggca cgataaagtt ggtgaggttt
     5221 gcgataggtc aattgatagt ggccaccctc acccttgata gagatagagg cttggtcaga
     5281 ctgagtaaca gccacttcaa catctggcag agaaatgtga ttttttaata cttcaatagc
     5341 ttgcgcttgt ttggaactaa gtcctgtaaa tcttaccatt ggttttcctc ctgtagccag
     5401 ttgacaaggg caccgtagag attagcatct gcatgatagg tgcaggcttg gataacgggt
     5461 gcgaccgtat attcttcgta agtatcgaca aagtcttcaa cagccttctt gacaccttga
     5521 ataaaatctg gattttgact gatagagcct cccagactga tgacatctgg atcaatcaga
     5581 tactggatat tgagcaagcc ttgcgccaaa ttgcggttca tgcgctcaat ggcttcttgg
     5641 caaatggcat tcccagctgc agcttcttgg taaatcttgc gaccgtccca atcagtctga
     5701 ccagattttt caatcacgta gcgaaccata ttcccagttg acgctagttg cgaccagttg
     5761 ttgagctttt cagcaggggc aagggttgtc atatagccaa attctccacc caagccgtgg
     5821 cgacctcggt gcagtctgcc attgataatc atagctccac caatccctgt cccaatcacg
     5881 acacaggctg cattttcaag ttctggatga gccagtaatt cactgagtcc aacgcagttg
     5941 gcatcatttt ccagatggac aggaatctga taagagctaa gcgcctcata ccaagaaaag
     6001 ccatggatgt agggcaccgc actgaagcca tcaatcacac ctgtctcttg attgaccgca
     6061 cctggaacac tcatagcaat cccactgtaa tcctgctctg acaagcgctg gtctagccaa
     6121 gctagtaaat cctccaagct ttcaggcgtt ggaatgcttg tcttattcag tatttttcca
     6181 tcaggagtca gactggcaaa cttaattcca gtccctccga tatcaatcgt tgcaatggtc
     6241 attgttttta ccataaaaag gggcgaatca gcagttagtt cttactcttt cgcccctcct
     6301 ttctctttat gtttactttt tgaaattaga tttcttcttt tttaatcaat tctgtacgaa
     6361 tttcttgtgg agccaatagt cctgaatgaa ctggatatgg tcgctcaagt aagtcaagaa
     6421 tggtttgttg atgttctgat atgcgcacat tttcttgact catattatag taacggagga
     6481 catagccttc ttcattttcc gccactttaa aggctgttgg acaaacttgc ggtatgctga
     6541 gaacagaatg gctcaagagg ctaccagtcg caaccacgct tccttcctgt ctagcaagct
     6601 gaaggctggt aaacggtgtc tgcaaggctt tggcacgacg ataggctgag aagcgttctt
     6661 gggcttggtg gcattcaatc gcaaattcga cttcaaactc ccgcaagcat tgtgcttctg
     6721 gcgttgggaa gtagccccag tcacctagct cacctgatgc acgcaaaatg gtcacggcaa
     6781 tggtgttatc cccaaggatt tcgtattcat tcaatccctt attggataca gtcacccctt
     6841 tttcatcgtc atacagactg acaaaagctt gttggtgttg aggattttca ggattttccc
     6901 atgaagcagc tggtttgttt ggtcgtgtca ccacctcata gatactttca gaatcattgc
     6961 ttggacgcgt gttatgagtc ttgaccaaga gacggatacg gtgatccttg gcagtgttag
     7021 taaagcgagt cttgaagcgg atttgtggat tgtcaacgaa gacagtcaac tcagtttcca
     7081 gaggaatgtt tgtcaattct tctgaccgtc cagcctcacg cttcataaac tcgatgatac
     7141 cttgttgctc ttcttctagc ttttcatccg cactgacagg cacggtcaat tcatgtttga
     7201 gcaagatttt agcatagcga tctgtgtttt ccaagacctc gtggccctta agctctgcaa
     7261 agattggctc tgttcctttt ggttggaaat agatatactc gtttccgatg tcaccccggt
     7321 cttcaaagcg gataaagtct tcataggctt cgtgagttgt cttgtcatag actgtgatgt
     7381 tgtcatccac actcaccgtt acgaatggtg tatcaatcac tccgttttgg taaataccct
     7441 cacggtgttc ttgttttcct tccagcaatt ggaaggttgt ccaagaaagc ggtgctaggt
     7501 gaactagaat ggtcacacgc acttgtcgag caatacgagc ttggcggaac ttatcttttg
     7561 gtaaatcgta ttcaaaatta gctccgaggt cttcgattgt agcctctaca ggacgaccat
     7621 ccaagtcctc cacacggtaa cttggcaaga taagagcagc catctttttg tagccttctg
     7681 ttgggtgcaa ttccttgaaa tcacaagtcg ccacatcaat cactgtgctg acagtatcga
     7741 ccttatcatg caagcctgtg ttaatgacag taaagagata gtcactttga gccttatccg
     7801 tagcaatttt acccttccac tcgttgagca aattactttt aacaaagttt cctacttggt
     7861 tgaccttggc aaaacgcgtt tccatctcgc ggtgaacttc gtccacgcta cagccacaga
     7921 tactatcatg tggcgcattc tgcaaaagtg ttttccaagc ataggtcaac tggtccttgt
     7981 ggttgtgtcc accagtgata atagtcaagg gttctaccac ttgctctagg aggttgctat
     8041 tttcttggaa ggcttgtttt aggtaaatac gggatgaaga agtgttggca agtgtgtacc
     8101 agccatctgt ttcctgactg gtcaactcgc ctgtaacagt tgataagtgt tcaggaagcg
     8161 caccttctac agcttgaaca tattcatcaa aagaactatg aacaaagatt acatccggga
     8221 agagttcatt tgccacacga atggcttcgc tcagattttt ctgtacaggc tggtggtcac
     8281 agccgttcat catcaaccat tggttggtcg aagcgtaggc acgcacatct gacaattttt
     8341 gtttccagaa ggtcaaggcc tcatctttgt caactggaat ttcattcccg ttactgtacc
     8401 agttggcaaa gagaatacct aaaacacgac taccatccac accctgccag tacatttctg
     8461 aaaactggga tgtaaactgc tcatcttcaa ggacttggtt gtcaaatcca atcggcttca
     8521 caccacgacc aaaggccgcc acgtgaatgc ctgatttttg aagaatttga ggcgcttgtc
     8581 ccatatttcc aaaggtatct ggaaagtagc caatctgggt tgatttaccc catttggcag
     8641 cttcttgttg accaatcaag gtattgcgga cattggcttc actggagatc aagtagtcat
     8701 cctgcaagat gtaaaaggga ccaattttaa gtttgccctc gtcaatgtag cgttggacct
     8761 tgtcacgatt ttcagggcga atttgtaagt agtcgtcaag gacaatagtt tgtccatcca
     8821 agtggaaact cttgaactca gggtcatttt caaagagatc aaagagattg tcaaacaatt
     8881 ccaccaactg catacgatgg ctttcaaaag gcaagtacca ctcacggtcc cagtgactat
     8941 gtgagataat atgtacaaca acattttcca tgaagtaaaa cctcattcta aatttaaatt
     9001 ttaacgtttt aaatgtaaac ttgtgattct gataagaatc ggttgagcta aagcgagccc
     9061 cttagcgaat atccaagtaa tccaagacca actcacagaa catcatgtta gcccaggaga
     9121 accattcacg agagtagagg gtcggatcat ctacatgaaa gctttcgtgc atgacacctg
     9181 taccaccatc gcaggcaacc agctgatcca gcaagaattt tttctctgcc ttatctcttg
     9241 ttgtcaatcc ttggatagaa agggcgattg gccagatata gcgatagaag gtatgagaac
     9301 ttccgagacc gctagcgtat tctccttggt agaaatatgg attttcagag ctcaaaatgg
     9361 tacgacgagt agcttgatac acttcatcat cgaccgaaca gtagcccaga tagggcgcag
     9421 ctagtagact tggtacattt ggatcatcca tgatgctggc atttcctagg ccatccactt
     9481 caaaagcgta aatcttttcg cccttgctgt tggtggtgta agcgtagttt ttgattcctt
     9541 cttggatttc atcctgaaga cgcttggcat cagcaataac actctggcta tcagctaggt
     9601 ttaatgctgc gaagatttct tgcacataac ccaagactac tacagcaaac atatttgacg
     9661 gaatcaagta actatactga cagcagtcat cgctcggacg aaaggctgac caagtcatac
     9721 ctgtcactgc aaagtcaggt ccaaagccat catttaccaa ggtgtcttcc ttacggtccg
     9781 tatctcggac aaaacgataa ggagagttct tgtggtcttg ttccaccgtc cacagatgga
     9841 gaatttcctt agtcgctgcg acaaaagtct catcaaactg actagtctcg ccagtctctt
     9901 tccagaggag ataagccaac tgcaaaggat agcaaagcga atccacctca tacttgcgct
     9961 cccagatcca gccgttaagg tctgtgtggt cagtctcgtg gtgccctttc cagttctcct
    10021 caatgttgaa ggagttagca tagggatcct tgagtaccaa ggtcatctga cgtttgacca
    10081 aacctgcaat ggtctgacgc aggagggcat ctcttttagc tacatgaagg tagggtctga
    10141 gttgggctgt cgaatctcga agccacatgg caggaatatc cccagtcaag acaaaagttg
    10201 aaccatcttc taagatttca accgtattgt ccaaggtatc ggtgtagcaa cgctcgaaaa
    10261 catccaccca ctctggatgg tccttagccc gctctgctac ttcatctagc cattctctaa
    10321 caatttcttt cgaataaatc atcgtgcaat tccctctttc aaacaagttt catgttcagt
    10381 atatagcttt tgagacagaa aatacataca caaatgatag tcatttttct acaaaatagt
    10441 tatttttgaa actccttttc taaaggccct ctttttctga tataatgtag aaaaacagct
    10501 aaaggaaaga ctatgaaacc actacttgaa accatcgata cccgctttgg tactgctagc
    10561 aagcatgcct tctctcgggg aaataccctg ccatacacag gcgtgccttt tgggatgaat
    10621 tactttgtgc cccagaccag tgaccagggt ggatcttggt tttttgatcc gcatctgcct
    10681 atctttcagg ggattcgatt aactcaccag cccagtcctt ggattgggga ctactcttgg
    10741 ctccttctga cacctgtcac cagtcaactg gggggagaca gcctctttca ccgccagtct
    10801 tcttatgata tagataaagc ttgcttccag cctcactatt tgaagctctt ttctctgcgt
    10861 tatcagattg aaacccagct cacaccgact tgctatggtg cttctattcg tttgaagcaa
    10921 aagcaaggca aagccctctc cctctatctt cacgcagcag atgaactgac agtagagcaa
    10981 gtagataagc ggactcttgc cctgcgacaa gaaggtaaaa ctgaaactaa caaaaattcg
    11041 ctaacgatgt tcactgccct gcaaatgaac acggatattc ttgctatcag ccaagaaggg
    11101 ggagactggc gaattgactt agcaagtagt caaaccgaga tgcaactagc cacttctttc
    11161 atctctcctt ctcaagcact gcttaatcta cctcaagaag attttgatag ctgtaaatca
    11221 agtgcccaag cggattggga aaatctcctc catcgttttg acattataga gataggagag
    11281 gctgaccgaa ccttctttga ccactgcctc tacagactct tcctcttccc acagactttt
    11341 tatgagatta atgaatcagg gcaagccatc cacatggatc tggctactgg tactgtcaag
    11401 cctggtgtcc tctttagcaa caatggtttc tgggatacct tccgcaccac cttccccctc
    11461 tttgccctta tcataccgga gcactatcaa cgctttttag aaggtttcct caatagctac
    11521 cgcgatactg gtttccttcc aaaatggctg gctccagatg aacggggtat gatgccaggt
    11581 acacttttag acggtattat cgcagatagc gcctgcaagg acatggcccc tgacctagaa
    11641 ggagaactct tccaagccat gctcgaaaca gccagcaaag ccgaccctct cggaatcaat
    11701 ggccgccacg gactagccca ataccaagaa ctaggttacc tctctaccga ccaccacgaa
    11761 agtgttagcc atactctaga ctatgcctat agtgactttt gtatcgccaa ctgtgccaaa
    11821 aaactaaaaa acatagaaat cgctgaaacc tacaaggctg cttcacaaaa ttaccgccag
    11881 ctatttgacg ctgagacagg ttacatgcga gcacgagaca atcaaggaaa ctttcaccct
    11941 gacttctctc cttatagttg ggggcgagac tacgctgaat gctctgccat tcaagctact
    12001 ttaggcgttc tccacgacat ccctggctta atccaactga tgggtggaaa agaaaccttt
    12061 agcaactatc ttttgaaagc ctgtcaagat gctcccctct ttgaaacaac aggctatggt
    12121 tacgaaattc acgaaatgag cgagatggct actgctcctt ttggacaaat agccatttcc
    12181 aaccagccta gtttccacat tccttatctc ttccgttaca gcgattaccc tgactacact
    12241 gcccttctta tcaagacgct ccgtcagaaa gcttttcacc caagttggga agcctatcct
    12301 ggagatgaag acaatggtag tctctctgct tggtacatct ggtcagctct cggattttat
    12361 ccaacctgtc caggaaaacc aagctatgac ctcggaatcc ctctctttga ccatctacga
    12421 gtctacctcg ctaaagaaga taaatggctg gatatccata ctaaacaaaa ccacaaccat
    12481 tttaactttg tcaaagaatg ccgactggac aaaacactcg tatcaactat tcaacaccaa
    12541 gacctcttaa aagctgaaca actaactttc accctcagct ggttaccaag tcattaataa
    12601 ccaaaagaac ctttgcatcg cgcaaaggtt cttcttttat gaaacttgga cttgaagttg
    12661 atccacaagc tgaccatcca ccgttaggtt cagatagaaa tccaaggttt tatctcctgc
    12721 gaaatacagg gttggtagat gcaaatcttt tttgctttcc cctgcttgga attgaaggac
    12781 ttgaatctca tcctgatagg cgacaccatg gacacctgtc cccggttgaa tcgaaatctt
    12841 agcctctaaa gaggagctac tttcactacg ttcaatccta aaatgaagag tctctccctt
    12901 tgccacagct aggctttttt ctgcaaatgc tagttcctga acaacttctt tttttgataa
    12961 tccaggagtt ttataaaggg aaatcttggt cagtagaggc atagattgtg cttcggtaat
    13021 catcacgcgc accttctgtg cctctactag cggacctcgc aagaggcgct tatgaccaac
    13081 tgtgaagccc ctgccaaatt cctgccagac accatccacc tctacttgca catggaaagc
    13141 agaaatacgt tggcctaact tcaaatcttc tctcaactca attacatcaa aagttttagg
    13201 agaacctaag tcgagttcta actggatggg caagtctgca tcgtttgccc aagagctggt
    13261 ctcaaggccg tctgtcaaat ggcgacaagc aaagtctgcg gaaagagctg gaccagatac
    13321 ctcagctccc agagccaaat cttctttata gagctcattg cgataggtcg caaattcata
    13381 aagtcgttca atatcctttg catcaaagag cccagcttga ttcggcggaa tattaagcaa
    13441 gagtggagtt cctcgcccta ctgagtgaaa gtagatttcg accaactcct cgagagactt
    13501 aggatcctga tcctcatggt agaaccagcc tggacggatg gaaacatctg cctctccgat
    13561 tgaaaaaatc gtgcccgagg gatccccgta ctgaagatag ttcagctctg cttctgttcc
    13621 tagtttatca ggattcacct tttgccacag tggatcacct gcataccctc gttcattgcc
    13681 aatccagcgg atactggtgc cttctgttga aaaaatcaag caatcgccct gcaggtcacg
    13741 aatggtttca aaccattttt caaattcata attaaccttt tgcgcgccct ctcctctggc
    13801 accatccatc caaacctcag cgaacttacc agcattccca tagttaggat ttgataagat
    13861 ttccttcaac tgagccagat aataggcatt gtagtccgct tctcggtcca catgatagag
    13921 gggactatgg gcatcccacg gtgacaggta gacccccata tccatatcaa actctgtggc
    13981 agcttgggat acttcaagga gcaagtcgcc ctttcctctc ctccaaggac tgaccttaac
    14041 cgaataatct gtgtgagctg tcggataaag gacaaagcca tcgtggtgct tgaccaccaa
    14101 aatcaacttt ttgaagcccg tttccttgag cacacgaacc cactcacgcg catccaactg
    14161 actcgggtta aagcgctcag gatcctcctg tccagtcccc cattcttggt cataaaaggt
    14221 attaggaccg aagtggataa aagctgctag ttcatctccc agataagcta gctgagtctg
    14281 acttggtaac ggtccatgcg gtttgatttt cttcatcgtt atatatctcc ttctttagca
    14341 acttctcttg ttcacaaggt tatccacagg ttgtggataa taaaaaatga tctaaccgcc
    14401 agctctttcc aactccaact ggtaggctca ggattttgct cctcaatgtt ccctcttcaa
    14461 acagctctgt catatggttc tgctaacacc tctcagaatg ctactctcca taattattct
    14521 caggcttctt tcaggtcatt gtgtgaagac cattcactct ccagactttt ccacagattg
    14581 tgaataagtg attattctaa actatcatct cgtcgactat aacaaaaaat aaaaagaggg
    14641 tcaataagat aattatctct tcctaaatac tcagaaaaga ttctataatt tgtacaattg
    14701 agcaagaaat gcttctccaa aatctatctt caaaagaatg ttgcatcttt ttatcctttc
    14761 acacaaacat ttctctttat acagatccaa gtgataacca gatgataatt ggacactttt
    14821 tggttatttg tgtatttttt gaaaaatttt ttaaaaaaat tttatatcct ctttaataga
    14881 agtttatgca tttgtatata tgtattccat cctatgttgt gtataataac ataaaattgt
    14941 gctaaatatc ataatttata ttattttccg agaaattata gccattgaca ttacctagga
    15001 attgcgttat aacatacatg taagcggtac ccaaaatggt gccaagtcaa aatttttaag
    15061 gaggaaaata catgtcttca catccaattc aggtcttctc agaaattggg aaactgaaaa
    15121 aagttatgtt gcaccgtcca ggcaaggagt tagaaaactt gttgccggac tatcttgaaa
    15181 ggcttctttt tgatgatatt cctttcttgg aagatgctca aaaagaacat gatgcatttg
    15241 cccaagctct tcgcgatgaa ggaattgagg ttctctacct agaacaactc gctgctgaat
    15301 cattgacctc tccagaaatc cgcgatcaat ttatcgagga atacttagac gaagccaaca
    15361 tccgtgatcg tcaaaccaag gttgctattc gtgaattgct tcacggcatc aaggacaacc
    15421 aagaattggt tgaaaaaaca atggctggga ttcaaaaagt tgaattgcca gaaattcctg
    15481 acgaagctaa agatctaact gacttagttg aatcagatta tccatttgca attgacccga
    15541 tgccaaacct ctatttcact cgcgacccat ttgcaacaat tggaaacgcc gtatcgctta
    15601 accacatgtt tgcagacact cgtaaccgtg aaacactcta cggtaagtat atcttcaaat
    15661 accacccaat ctatggcgga aaagtggatt tggtctacaa ccgtgaagaa gatacgcgta
    15721 tcgaaggtgg agacgagcta gttctttcta aagacgtcct tgcagtaggt atttctcaac
    15781 gtacagacgc agcttctatc gaaaaacttt tggtcaacat cttcaagaaa aatgttggct
    15841 tcaagaaagt tttggccttt gaatttgcta acaaccgtaa attcatgcac ttggatactg
    15901 tcttcactat ggtagactat gacaagttca ctattcaccc agaaatcgaa ggcgaccttc
    15961 acgtttactc agttacttac gaaaacgaaa aacttaaaat cgttgaagag aaaggtgact
    16021 tagctgaact tcttgctcaa aaccttggtg tagaaaaagt tcatttgatt cgttgcggtg
    16081 gtggcaatat cgtagcagct gcgcgtgaac aatggaacga cggttctaac actttgacca
    16141 tcgcacctgg tgtggtagtt gtttatgacc gcaataccgt gaccaataag attttggaag
    16201 aatacgggct tcgcttgatt aagattcgcg gaagtgaatt ggttcggggc cgtggtggac
    16261 ctcgttgtat gtctatgcca tttgaacgtg aagaagtgta atcgctgttc ggcattcgtc
    16321 aatagaaaat gtaaaaaata gaaagaggaa gtaataaaat gacaaattca gtattccaag
    16381 gacgcagctt cttagcagaa aaagacttta cccgtgcaga gttagaatac cttattggtc
    16441 tttcagctca cttgaaagat ttgaaaaaac gcaatattca acaccactac cttgctggca
    16501 agaatatcgc tctcctattt gaaaaaacat ctactcgtac tcgtgcagcc tttacaactg
    16561 cggctatcga ccttggtgct cacccagaat acctcggagc aaatgatatt cagttgggta
    16621 aaaaagaatc tactgaagat actgctaaag tattgggacg tatgtttgac gggattgaat
    16681 tccgcggatt cagccaacgt atggttgaag aattggcaga attctcaggc gttccagtat
    16741 ggaacggtct aactgacgaa tggcacccaa ctcaaatgct cgctgactac ttgactgttc
    16801 aagaaaactt cggtcgcttg gaaggcttga cattggtata ctgtggtgat ggacgtaaca
    16861 acgttgccaa cagcttgctc gtaacaggtg ctatccttgg tgtcaatgtt cacatcttct
    16921 caccaaaaga actcttcccc gaaaaagaaa tcgttgaatt ggcagaagga tttgctaaag
    16981 aaagtggcgc acatgttctc atcactgaag atgctgatga agcagttaaa gatgcagacg
    17041 ttctttacac agacgtttgg gtatcaatgg gtgaagaaga caaattcgca gaacgtgtag
    17101 ctcttcttaa accttaccaa gtcaatatgg acttagttaa aaaagcaggc aatgaaaact
    17161 tgatcttcct acactgcttg ccagcattcc acgatactca cactgtttat ggtaaagacg
    17221 ttgctgaaaa atttggtgta gaagaaatgg aagtaacaga cgaagtcttc cgcagcaagt
    17281 acgctcgcca cttcgatcaa gcagaaaacc gtatgcacac tatcaaagct gttatggctg
    17341 cgacacttgg taacctttat attcctaaag tataatttta gataataaac cgtctaccaa
    17401 cagctatgag ggctgcgact aatagcttta gtccggtcct cttttatgta atggtaatct
    17461 attatttctt ataaaatatg tgaaaaatca ttaaattgaa atctaaacgc attctattga
    17521 gtgtgataaa ggagaattta tggcaaatcg taaaattgta gtagctttgg gaggaaatgc
    17581 gattctttct tctgacccat cagcaaaggc tcaacaagaa gctttagttg aaacagctaa
    17641 gcatcttgta aaattgatta aaaatggaga tgatttgatt atcactcacg gtaatggacc
    17701 tcaagttggg aatctcttgc tccaacattt ggcatcagac tctgaaaaga accctgcctt
    17761 cccactcgac tcacttgtcg ctatgacaga aggtagcatc ggtttctggt tgaaaaatgc
    17821 tttgcaaaat gctctcttgg atgaaggcat cgaaaaaaat gttgcctctg ttgtaacgca
    17881 agttgtcgta gataaaaatg atccagcttt tgttaacttg agtaaaccaa tcggtccttt
    17941 ctattcagaa gaagaagcaa aagcagaagc cgaaaaaagc ggagcgactt tcaaggaaga
    18001 tgctggccgt ggctggcgta aggtcgttgc ctcaccaaaa cctgttgaca tcaaagaaat
    18061 tgaaaccatc cgtactcttt taaataatgg tcaagtcgtc gtagctgcag gtggtggcgg
    18121 tattcccgtc gtcaaagaaa acaatggaca tttgactggt gtcgaagcgg ttattgataa
    18181 agacttcgct tcccaacgtt tggcagaatt ggttgatgca gacctcttca tcattttgac
    18241 aggtgtagat tatgtatttg ttaactacaa caagccaaac caggaaaaat tggaacatgt
    18301 gaatgttgcc cagctggaag aatatatcaa acaagatcag tttgcaccag gtagcatgct
    18361 tccaaaagta gaagcagcta tcgcttttgt caatggtcgt ccagaaggaa aagcagttat
    18421 tacttccctt gaaaatctag gcgccttgat tgaatctgaa agtggaacaa ttattgaaaa
    18481 aggataagtt gttttactaa taagatgtat tctatttcta gtatctttat atcaaattag
    18541 aaattattct tgaaaacatg tacaatattt caaaagatat tagtttttta gactttaata
    18601 tggtaaaaca aatataaata gaaagcgttt tcttaaatgt ttatttaaga aagtagttgg
    18661 ttttttacac tttgttagac atcaggagga aaaacaaatg agtgaaaaag ctaaaaaagg
    18721 gtttaagatg ccttcatctt acaccgtatt attgataatc attgctatta tggcagtgct
    18781 aacttggttt atccctgcgg gggcctttat agaaggtatt tacgagactc agcctcaaaa
    18841 tccacaaggg atttgggatg tcctcatggc accgattcgg gctatgctag gtactcatcc
    18901 agaggaaggt tcgctcatta aagaaacgag cgcagcgatt gatgtagcct tcttcatcct
    18961 tatggttggt ggtttccttg gcattgtcaa caaaactggt gctcttgacg tagggattgc
    19021 ctctatcgtg aagaagtata agggccgcga aaaaatgtta attttggtac tgatgccttt
    19081 gtttgccctc ggtggtacaa cttatggtat gggtgaagaa acaatggctt tctatccact
    19141 ccttgtgcca gttatgatgg ccgttggttt tgatagcctg actggtgttg caattatttt
    19201 gctcggttct caaatcggct gtttggcatc tactctgaat ccatttgcga caggtattgc
    19261 ttcagcgact gcgggagttg gtacagggga cggtatcgta cttcgtctga tcttctgggt
    19321 taccttgact gctcttagta cttggtttgt ttaccgttat gcggataaga ttcaaaaaga
    19381 tccgactaag tcactggttt atagtactcg caaagaagat ttgaaacact ttaacgtaga
    19441 agaatcttca tctgtagaat ctacacttag cagcaaacaa aaatcagttc tcttcttatt
    19501 tgtgttgaca ttcatcttga tggtattgag cttcattcca tggacagacc ttggcgttac
    19561 catttttgat gactttaata cttggttgac tggtcttcca gttattggta atattgtcgg
    19621 ttcatctact tctgcactag gtacttggta cttcccagaa ggcgcaatgc tctttgcctt
    19681 tatgggtatc ctgattggtg ttatttatgg tcttaaagaa gataagatta tctcttcctt
    19741 catgaatggt gctgctgact tgctcagtgt tgccttgatc gtagcgattg ctcgtggtat
    19801 tcaagttatc atgaacgacg gtatgattac cgatacaatc ctcaactggg gtaaagaagg
    19861 cttgagcggt ctatcttcac aagtctttat cgttgtaact tatatcttct atctacctat
    19921 gtcattcttg atcccatctt catctggtct tgccagcgca actatgggta tcatggctcc
    19981 acttggagaa tttgtaaatg tccgtcctag cttgattatc actgcttacc aatctgcttc
    20041 aggtgtcttg aacttgattg caccaacatc tggtattgtg atgggagctc ttgcacttgg
    20101 acgtatcaac attggtactt ggtggaaatt catgggcaaa ctcgtagtcg ctattattgt
    20161 agtgaccatc gcccttcttc tccttggaac cttccttcca ttcctataaa atagtgagtg
    20221 aggtgattcc atgaaaatag atataacaaa tcaagttaaa gatgaatttc ttatatcatt
    20281 aaaaaccttg atttcctatc cttcagtact caatgaagga gaaaatggaa caccttttgg
    20341 acaagcaatc caagatgtcc tagaaaaaac tttagagatt tgtcgagaca taggtttcac
    20401 tacctatctt gaccctaaag gttattacgg atatgcagaa atcggtcagg gagcagagct
    20461 tctggccatt ctctgtcatt tggatgttgt tccatcaggt gatgaagcag attggcagac
    20521 accgccattt gaagcaacta tcaaagacgg ctgggtattc ggacgtggtg tccaagatga
    20581 taaaggccct tcgctcgcag ctctctatgc agtaaaaagc ttgctggacc aaggtattca
    20641 gttcaaaaag cgcgtacgct ttatctttgg taccgatgag gaaaccctct ggcgctgcat
    20701 ggcacgctac aataccatcg aagaacaggc cagtatgggc tttgcacctg actcatcttt
    20761 tcctctgacc tatgctgaaa aagggcttct acaggtcaaa cttcatggcc ctggatcgga
    20821 tcaactagag cttgaagtag gaggcgcctt taacgttgta ccagacaagg ccaactacca
    20881 aggtcccctc tatgaacagg tttgtaacgg tctcaaagaa gctggttatg attaccaatc
    20941 cactgaacaa accgtaacgg ttctcggagt gccaaagcat gctaaggatg ctagtcaagg
    21001 tatcaatgct gtcatccgac tagctaccat tcttgctcct ctccaagaac accctgctct
    21061 cagttttctt gcaacacaag caggtcaaga cggcacagga agacaaatct ttggtgatat
    21121 agcagatgaa ccttctggtc acctatcctt taatgtcgca ggtctcatga tcaatcatga
    21181 acgttctgaa atccgtattg acattcggac tcctgtctta gctgacaagg aagaactagt
    21241 agagttgctt acaagatgtg cacaaaacta ccaactccgc tacgaagagt ttgactatct
    21301 agcgcctcta tacgtcgcaa aagacagtaa actcgttagc acactgatgc aaatctacca
    21361 agaaaagact ggcgataaca gtcctgctat ttcatccggt ggtgccactt ttgctcgcac
    21421 catgccaaat tgtgtagcct tcggcgcctt attcccagga gcgaagcaaa cagaacatca
    21481 ggcaaatgaa tgtgccgttc tagaagattt gtaccgtgct atggatattt atgccgaagc
    21541 cgtctatcga cttgcaactt aatcaggcaa ctgtttctac caaaaaaaat cgaccgatta
    21601 atgaactgca ccccaaaagt tagacagaa
//
LOCUS       DBEROU010000040        21241 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.040, whole genome shotgun sequence.
ACCESSION   DBEROU010000040 DBEROU010000000
VERSION     DBEROU010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 21241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21241
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.040"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(194..583)
                     /locus_tag="ACL8CL_001614"
     CDS             complement(194..583)
                     /locus_tag="ACL8CL_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGS1330836.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNTSGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(637..1344)
                     /locus_tag="ACL8CL_001615"
     CDS             complement(637..1344)
                     /locus_tag="ACL8CL_001615"
                     /inference="COORDINATES: protein motif:HMM:NF019142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HGS1330837.1"
                     /translation="MFRRNLNTSFAHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVK
                     VATKPKVVTKKISSTRIRFEKDETKDRSENHVTIDGEDGYVTTTRTYDVNPETGHVTE
                     KVTVDRKEATDTVIKVPAKSKVEEVLVPFATKYEADNDLSAGQEQEITLGKNGKTVTT
                     ITYDVDGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEISIETEYLDGPTLDKSQE
                     VEEVGEIGKLLLLQSIL"
     gene            complement(2043..2594)
                     /locus_tag="ACL8CL_001616"
     CDS             complement(2043..2594)
                     /locus_tag="ACL8CL_001616"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1330838.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSAENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKVYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(2892..3716)
                     /gene="nadE"
                     /locus_tag="ACL8CL_001617"
     CDS             complement(2892..3716)
                     /gene="nadE"
                     /locus_tag="ACL8CL_001617"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HGS1330839.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKNFV
                     LGISGGQDSTLAGRLAQLAIEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(3713..5173)
                     /locus_tag="ACL8CL_001618"
     CDS             complement(3713..5173)
                     /locus_tag="ACL8CL_001618"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HGS1330840.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIHNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLCNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFTEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DETGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(5289..5777)
                     /locus_tag="ACL8CL_001619"
     CDS             complement(5289..5777)
                     /locus_tag="ACL8CL_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6773 family protein"
                     /protein_id="HGS1330841.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(5767..5976)
                     /locus_tag="ACL8CL_001620"
     CDS             complement(5767..5976)
                     /locus_tag="ACL8CL_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1330842.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(6362..7090)
                     /locus_tag="ACL8CL_001621"
     CDS             complement(6362..7090)
                     /locus_tag="ACL8CL_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1330843.1"
                     /translation="MKLNKLNFLKENIRNLYSSGVIYLSLLISFIPPILLTFFILKTQ
                     ETSLGIKHISNFYAMLGMLMAVIHANRVISRDFSHNTVSLFYNQQKNRMIYVLSNFLY
                     AISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIVAIVVNTILLVLFYFLLSYIFYLYK
                     LKSGLVFGILVALLLFIPNILNTIMMNTSNDLFIKAIELLPFYSLPLFVVSNTMSISQ
                     YLVLITTIILLYFFTLKKSKKYSF"
     gene            complement(7106..7976)
                     /locus_tag="ACL8CL_001622"
                     /pseudo
     CDS             complement(7106..7976)
                     /locus_tag="ACL8CL_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8332..9060)
                     /locus_tag="ACL8CL_001623"
                     /pseudo
     CDS             complement(8332..9060)
                     /locus_tag="ACL8CL_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
     gene            complement(9306..9617)
                     /locus_tag="ACL8CL_001624"
     CDS             complement(9306..9617)
                     /locus_tag="ACL8CL_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHY zinc finger protein"
                     /protein_id="HGS1330844.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCPFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(9632..10918)
                     /locus_tag="ACL8CL_001625"
     CDS             complement(9632..10918)
                     /locus_tag="ACL8CL_001625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1901576 - obsolete organic substance
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HGS1330845.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMIDHIPDMIENGVDSLKIEGRMKSIHYVSTVTNCYKAAVDAY
                     LESFEKFEAIKQDLVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVV
                     SYDDAAQTATIRQRNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELL
                     TIKVPQPVQSGDMVRALKEGLINLYKEDGTSVTVRA"
     gene            11515..12084
                     /locus_tag="ACL8CL_001626"
                     /pseudo
     CDS             11515..12084
                     /locus_tag="ACL8CL_001626"
                     /inference="COORDINATES: protein motif:HMM:NF021048.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ApaLI family restriction endonuclease"
     gene            12092..13282
                     /locus_tag="ACL8CL_001627"
     CDS             12092..13282
                     /locus_tag="ACL8CL_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HGS1330846.1"
                     /translation="MTVLKGDNLEILKTIESSSIDLIYMDPPFFTQKTQKLSNNKNIM
                     YSFEDTWTSIEDYKEFLSVRLEECKRVLKNSGSIFVHCDKIANHHIRLILDNIFGADM
                     FQSEIIWNYKRWSNSKKGLLNNHQNIYFYSKSKDFKFNTIFTEYSSTTNIDQILVERK
                     RDGNSKTIYKVDNNGNYILAKEKNGVPLSDVWNIPFLNPKAKERVGYPTQKPILLLEQ
                     IIKIATDKNDIVLDPFCGSGTTLVASKILNRNYMGIDLSEEAINITQQRLENVIKTSS
                     NLLNKGIEAYRTKTEEEENILKLLQAKIVQRNKGIDGFLPKHFQKKPIPIKIQKNNEC
                     LNESISLLQNAINSKKLDFGVVIKTHSDNLLFDFDTIPENIIVVDHFELTIEKWLSKS
                     QQLL"
     gene            complement(13797..14789)
                     /locus_tag="ACL8CL_001628"
     CDS             complement(13797..14789)
                     /locus_tag="ACL8CL_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1330847.1"
                     /translation="MSYYDFVKDYMKVDECKNNEKYSSAGHTFCWKKENSTYAEGLYW
                     FYEGGSFIIDIHDFYIKEEVVQNSTYSMSDYVSIYSSYIVSANGEKFNPYQTITANSL
                     CTFDFDNIKDDFVFLLHENCCYLAVSIGFKKELIEKHLASININPESFYSTLLQPNQI
                     ILTKALERVAMEILNCKMDAPAADFFFKAKANEWISIVIDTYLNRKKYKIEIDDDKAL
                     EDVARFLDDHFATNVNQGTLEKISKMSGTKLKNLFKEKYCQSITEYTQRKRMNVAETL
                     LLNTELPIKEIAESVGYSSASKFSIYYKRYKGKLPSEVRNLACKHHNFKCDYCD"
     gene            14949..16703
                     /locus_tag="ACL8CL_001629"
     CDS             14949..16703
                     /locus_tag="ACL8CL_001629"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330848.1"
                     /translation="MKIYKKLFAYVQDKKYLGVLAIIFSAISALLTVYGYYLIYKFLD
                     KLIINSNLSGAESIALKSVITLTSGAIFYFVSGMFSHILGFRLETNLRKRGIDGLEKA
                     SFRFFDLNPSGQIRKIIDDNAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGI
                     ILLALTIIGGLILGAMMGEQEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFK
                     SFYKAIKDYSKYAYDYSLSCKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLE
                     LIMILFLSGVLFVSFMRMMWYSMYISQGNYAVDTLEALYEDMQKDKLVHGNINNFKNY
                     NIEFENVSFAYNDKAVIENLSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSI
                     KIGGIAISEYSDEALIKAISFVFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGC
                     DLILDKFPERENTIIGSKGVYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEF
                     ELQKAFKNLMKDKTVIMIAHRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSL
                     QEMFNSANEWRVSNERVL"
     gene            16687..18408
                     /locus_tag="ACL8CL_001630"
     CDS             16687..18408
                     /locus_tag="ACL8CL_001630"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019214631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330849.1"
                     /translation="MKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAILLMIFA
                     QEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTAENLSKL
                     PLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAGNVKMGL
                     AVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYNLSKDIK
                     DDLYKKMEDSERVHLKAEVTTILTLSISSIFSFISLAVVIFVGVNLIINKEINSLYLI
                     GYLLAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDYSLKKFDIDLKD
                     VEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYDYDKGQILIDGK
                     DIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRAAKLANCTDFIE
                     KMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASLDVNNEKKIQES
                     LNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKSKIYKNLIEKTK
                     MAEEFIY"
     gene            18419..19048
                     /locus_tag="ACL8CL_001631"
     CDS             18419..19048
                     /locus_tag="ACL8CL_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGS1330850.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFSNGLYCVGYFTPCLILWCYLN"
     gene            18949..19506
                     /locus_tag="ACL8CL_001632"
     CDS             18949..19506
                     /locus_tag="ACL8CL_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HGS1330851.1"
                     /translation="MYRQGSIEKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIP
                     IVIRMFILPFMAASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKN
                     IKMAMRVRGINFKNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKTRYIRV
                     KIQLIDFVYVLAVAGLIVGGLIWLK"
     gene            19494..20858
                     /locus_tag="ACL8CL_001633"
     CDS             19494..20858
                     /locus_tag="ACL8CL_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1330852.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGSGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDESSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDRVFLIDKGKLK
                     KTYTRSEFLKLDKNELNALSLRDKKLSKLKVPYLKEGGEYQIKNLSYKFTDDECLSLK
                     DISFKLGKIYGIIGSNGRGKSTLLRCLIGLEKKSKEEIYFKGEKLSKKERLKNSSLVM
                     QDVNHQLFTDEVFNELRLGVKNFDEEKAKIILKDLGLDEFIERHPMSLSGGQKQRLAI
                     ASVMCKNSPFVFFDEPSSGMDYSNMIKISELINKYKTMDKIIFIVSHDIEFLNEVADE
                     IFEL"
BASE COUNT         7149 a         3686 c         3535 g         6871 t
ORIGIN      
        1 ctagttttct agtttgctct ttgattttca ttgagtatta aacaaaaagt gaacaaatct
       61 gaattctaat gtacagaaga ctaggcttgt tcactttttt atagtcgcta taagatgacc
      121 ttatctatag ctttttatat ataattatat attcagacat actattatca attttgtcgc
      181 agggaggaat ctgttaacgc acccattcac cattatcatt gactctatag ccatctatac
      241 ttgtattgac cgctaactca cccgatgtat ttacataata ccatttacca ccaacttgga
      301 accattgatt gactttcata gaaccgttgc tgttgaggta gtaccatgaa ccattaactt
      361 gtacccaacc tgttgccatg gaaccatcag tattataaaa ataccacata ccattttctt
      421 gtttccagcc tgttgttgga gcaactgctt tagctggttc tactgctaca tctgttcctt
      481 ggttagatgt aacagataca ggatacgaag gaatagatga ttgctcagga acaacaactt
      541 tttcaggttc tctcgtccct ctccttatac gtctttttac catctcttta gtaatttgac
      601 gagaagtagt ttcttcaatt gttccatcac gttcatctac agtatagatt gtagtaagag
      661 taatttacca atttctccta cttcttctac ttcttgactt ttatcaagag ttgggccatc
      721 gagatattct gtttcgattg aaatttcttg gacaagaact tggggcttgg ttcctttttt
      781 aacaactctt gtttgagagt cttctttttg acttaaagta ctctcagtta cttgtccact
      841 ctttccatct acatcataag ttatcgttgt aactgttttc ccattctttc ctagagtaat
      901 ctcttgctcc tgtcctgcag aaaggtcatt gtctgcttca tatttagtag caaatggaac
      961 aagaacttct tcaaccttgc ttttagctgg aactttgata actgtatccg tggcttcttt
     1021 tctatcaaca gtaacctttt cggtaacatg accagtctct ggattaacat cgtaggtcct
     1081 tgtcgtggtt acatagccat cctctccgtc aattgtaaca tgattttcac tacggtcttt
     1141 tgtttcatct ttttcaaaac gaattcgcgt acttgaaatt ttcttggtta ctaccttagg
     1201 tttagtcgct acttttacaa taatatcccc attgtcagcg tcatcatact ctattccctc
     1261 ttctttatct ctagtatcat ctctgacata ttgaatccca tcagcagcat gagcaaaact
     1321 tgtattcaga ttcctcctaa aaataaagtt agcccgatta ccgcagaacc aaaaatcttt
     1381 ccgagtttac gtattgcata gcgcttatta gtattagatt ttgccattac atcctacttc
     1441 tagtatagca tcttttctat caaacgttaa acaatatact ttatatataa aatagactta
     1501 gaatgatata tttactattg aactaacact ttaactatat cgtaatcaat ctcatatata
     1561 aaggattgca gacatctgat ctaaatacat gctaatatat ttagatacaa acattccaac
     1621 ttgataattt tcactcatct ttcatcattc cttacacaac gatgcagtat aaatagaata
     1681 gttttctcat cagaatgaga ctattttaat attagataat ttgatagaaa aacataaata
     1741 aaattggttg aatttaattc cctaactcga tagaggattc tattttaaat ttaacaacag
     1801 ctaggattac actataaaaa gtacattacc acctgttcgg ctggagaacc tagtcgatta
     1861 gctccctctt agttacaagc ttcaagcgat tttttaacta atactaaacc agattaaaga
     1921 aggaactaga gcatctgtct tgtaataatc aatatgctat tttaaaattg caaataaact
     1981 tggaatgcta attattatat ttacaataaa tagttgactt cgatagaaac agaaaataga
     2041 attcaacatt cttccctact tataccataa taaataagat ccgcaagata gccttttctc
     2101 tctacaccat taacaatggt ttgtagatag gacattccag ccttttccat gacacgacct
     2161 gaagctgggt tgagactggc ataacgtgct ctgacctttt gaaaacctgc ttgagtaaaa
     2221 caaaagtcca agacagcttt caaggcctct gtcatcatac catgtcccca gtaaaccttg
     2281 cctagcacat agccgatttc acagcttaaa tcagcctcgt ctatcttaac gatgctgata
     2341 tctcctatca cttgctctgg gttttctttt agacaaatgg cccatttgta atagttgaga
     2401 ttagtatagg aagcaaccca attgcgaatc gagtttcgag tgatttcgac atcaggatgg
     2461 ggatcccagg taacataggt caaattctca gcggatgaag cccaattttg aaacatagct
     2521 tctgcatcac tctccacaaa tcttcttaaa atcaaacgtt ctgtctgtaa tatttgcgta
     2581 ccgatagatt tcatgatgct acctcacttc cttatttcta tgctcgtagt aaaaaggctc
     2641 aactcagtaa cagctagaaa acgagtttgt cttctaattt cttgacgcag tcgctgcctg
     2701 gtttgaaatg cgctctagca agctttttca aacctagtca tcgttgcagg ggtaagacga
     2761 tgaaatcgaa ctcttcgagt taccgaatct gcctcatttt cttatttcta aactcggggt
     2821 tcaagatccc ccagaccttc tctcactttc ttatttcaag atgagaggct gaaaacctcc
     2881 aatggaggtt tttactccca aaagtcatca aatacggtga tgggtaagtg gcgtttgtgt
     2941 tggcctttgt gccaccagtt ttcaatggtc gcttgagctt ctgggctgat tgttttgcct
     3001 tctaggtagt cgtcaatctc tgcgtaggtg actccaagtg cgacttcgtc agctaggcct
     3061 ggtttatctt cttctaggtc tgctgttggg attttttcat aaagggctgg ctctgcgcca
     3121 agtttctgca agagctgttt tccttggcgt ttattgaggc ggtaaagagg gagaatatcc
     3181 gcaccgccgt caccaaactt ggtaaagaaa cctgtgatat tttccgcggc gtggtctgtt
     3241 ccaatgaccg ctccgctatg ggaaccagca agggcatact gagcaatcat acggcaacgt
     3301 gccttgatat tccccttgtt gaagtctgaa acaggactac ctgtcgcttc aactgcagct
     3361 gtcatagcat cagctgactc cttgatattc acaaccaagc tgacatctgg ctggatgaag
     3421 gctagggctt tttgagcatc tgcttcatca gcttgcactc cgtatggcag gcggacagcg
     3481 ataaatttgt agctgtcgtc tcccgtttca gctcgcagtt cttctatagc taattgcgcc
     3541 aaacgtcctg ccaaggttga gtcttgtccc ccagaaatcc ctagtacaaa gttttttagg
     3601 aagggatgtt ttttcagata tctttttaag aaatcaatag aacgacggat ttcttcctgg
     3661 gcatcaatca ctggtttgac acccagctct tggataatcg tttcttgcaa actcattctt
     3721 cttctccttc accaagggct tccttgcgca tcttatcaat caagtccatc ttatcttgcc
     3781 atacatcacg tgccaaatcc actggatagt gctgcggatt gagcacacgc ttatactcat
     3841 cccacaactt gtcaaattct ttacgggcat aatcctgaat gtcagtcaaa ctaggcaagt
     3901 tgtaaactaa tattccttct ttgaagatat ccaccaagag aggaacggca tcaaaattac
     3961 gaaccgtctt cttgatgtat gtataggtcg gatggaacat cttgatttct gtcatgtcgc
     4021 taatatccac accatcataa gtgatgtagt caccttctga cttgcctttt tcacgactgg
     4081 taatgcgcca cacctgcttc ttacctggcg tcgacacttt ttccgcatta ttagacagct
     4141 taatcgtatt gcgcatctga ccagtttcat cttcaattgc aacaatcttg taaaccgccc
     4201 caagagccgg ctggtcatag gctgtaatca gcttggtacc cacaccccag acatcaatct
     4261 tggccttttg catcttgagg ttaaggatgg tattttcatc tagatcatta gaagcataaa
     4321 tcttagcctc tgtaaatcca gcctcgtcca gttgctgacg gactttctta gaaatgtaag
     4381 caatatcccc agagtcaatc cgcacaccca taaagttaat ctgatcaccc agttcacgcg
     4441 ccacctgaat ggcagctggt acaccgatgc gaagggtatc ataggtatcc acaagaaaga
     4501 cacaattttt gtgggtcgca gcgtaagcct tgaaagcctc atagtcattg ccataaacct
     4561 gcaccaaggc atgggcatgg gttcccaaaa caggaatgtc aaagagttta cccgcacgca
     4621 cgttgctggt tccattggcg ccaccaatca ccgctgcacg tgttccccag atggccgcat
     4681 ccatttcttg agcccgacgt gtcccaaact ccatcaaggg ttcatcttcg ataaccgaac
     4741 gaatacgagc tgccttcgtc gccaccaagg tctggtagtt gacgatgttc aaaagagccg
     4801 tttcgaccaa ctgacattgg gctagaggtc cttccacctg cacaatcggt tcattagcaa
     4861 aaaccaaatc cccttcttgg gcagaacgaa cggtcaactc caacttgaaa ttgcaaaggt
     4921 aatccaagaa cgccccatga taaccaagcg actccaaata ggctatatca ctatctgaaa
     4981 aacgcaagtc ttcaagatag ttcacaattc tttccaaacc tgcaaaaacc gcatagccgt
     5041 tcttaaaagg ctgttggcgg aaatacacct caaagaccgc cttcttattg tgaatccctt
     5101 ggtcaaagta aacctgcatc atgttgatct ggtacaagtc cgtgtgcaat gtcaaactat
     5161 catctggata catacttttc ctacttcctt agctagaaac ccatgaaaat tttcaagaac
     5221 tttcatgtat tccaataaat tagtactatt atatcacatt ttagctggat tgagaaaaga
     5281 gtaacaagct attctccact ctccaattca tccatatctt gttcaaattt tttctgagcc
     5341 cattcgccat agctcttaag accaagattg ccaataaaga cccacggaag gtaaatgaca
     5401 taagtaatga cccaagcaga caggtattta aaattcaaag gattgtgctg ataaatttct
     5461 atgttgaatt gataattctg caacatcaaa agagccgtaa tagccaaggt taggaaaaaa
     5521 caacccaaaa tcgtaaaatg aaaacgacta tagtaggtca ctcccagata acgggcgcga
     5581 ttgaaaaagt aaaatgtccc tatgatgata acgattagca gcatattaga attaaaaagg
     5641 cttggtgcta atactgaaat gatataagat aggagcgaca aagcaatgca gatatagaaa
     5701 ctttcagatc ccgctttatt gaacagttgt tcttctcttt cgtctagtaa ttgataataa
     5761 taaaatctat ttttcatctt cttcctccca aaatagttgg tctagggttt tccctaaaca
     5821 tctgcaaata gactggcaga gcgagagact gggattgtat tttcccgcct ctatcaaacc
     5881 aatagtctgg cgtgtcaccc cgacagcctc tgccagttga ccttgtgtta aatcacgctc
     5941 tacccgagct aattttaatt ttaaattttt agccaccttc gtcctcctta tagttttaat
     6001 actcatctac tcttaaaaaa tccaaaacca acacaagcta tcagctattt attataaccc
     6061 cattaatttg cactccagta ttatcttcag acgaaaatca gtattttagg ttgtttgaaa
     6121 taaatttcct agtttactct tttattttta tttagtatta gctttagtag aatcttttaa
     6181 acgagttttt attgcttata tttcttgttt agtttagttg gtttcatatc actattatat
     6241 aatgcttttg gaaatgagcc tagtataagt caaaaacgtg ttgaatagtg aaagcaggag
     6301 cataccaaga tgactgattt ttttactgta ttttaggtat gcaaaataat actaaatgaa
     6361 actaaaatga atacttcttg cttttcttga gagtgaaaaa gtacaataaa atgattgtag
     6421 tgattagcac aagatactga ctaatagaca tcgtatttga aaccacaaat agaggtaaag
     6481 aataaaaagg aagaagttca attgctttga taaacaaatc attactagta ttcatcataa
     6541 tcgtatttaa tatattaggg ataaagagta gtaaagctac taaaatacca aataccaagc
     6601 cacttttcaa tttgtataaa tagaaaatgt aagataatag aaaataaaac aggactaaca
     6661 aaattgtatt gactacaata gctactataa aatctaatcc taaatcacct ggaataccca
     6721 acttacttac tatgactagt aacacaatgc cattcaaagc ataaataatg gaaactgaga
     6781 tggcatatag aaaattagac aagacgtaaa tcatccgatt cttctgttga ttataaaaca
     6841 aacttaccgt attgtgggaa aaatctctac taataactcg attagcatgt ataacagcca
     6901 ttaacatacc gagcatagca taaaagtttg aaatatgctt aataccaagc gatgtctctt
     6961 gagtctttag aataaagaat gtaagcaata tcggcggtat aaacgagata agcaaactga
     7021 gatatattac gcctgatgaa tataaatttc ttatattttc cttaagaaaa ttcaatttat
     7081 ttaatttcat gattataacc ttcctttatt ttgaacgatt taagtaaata tctttaagcg
     7141 tctctttttt agtttcaaaa tcaactactt taatagagtt ttcgttaaaa aatttaaaaa
     7201 gctcactact ttgaatattc ccagacatag taattctcag tccctcttcc tgaacaatat
     7261 ccccaaattc ttgtttggta atgaaaatgt ctctatctgt agctgatgaa aaagctatct
     7321 caaacaagaa attatgacta tcttttccta ctttttgaaa tgtcaaaagc ccgctctcca
     7381 agaaaagaac cctctcacaa atttcttcaa tgtcttctaa tttatgactc gatattaaaa
     7441 ttcccacgtt ttcatttaaa gctaattttt ttagaaccgc taaaactatt tgtgatgatt
     7501 caatatctaa accattagtc ggttcatcta aaatcaatat atcaggtttc gtaacgagag
     7561 ttagaagcaa agctaatttt tgttttgtac ccaaagaata ggtctttact tttttattaa
     7621 tagactgagt caaatctaac tcttggatca aacttctaaa tctttcttga ttgtagtcaa
     7681 caccatataa atttgacaaa tattttaaat tctctaaacc tgttttagat aaaaataatt
     7741 ttggttcttc gattaaatac ccaatattat cactgcttat aatattacct gaagtcggtt
     7801 gattattctg aacaagaatt ttcatcaacg tactcttacc aactccattt ctccctacta
     7861 gaccaacaat ttcacccttg ttaagtgcaa aatttatatc tttgagaatg attgaatgac
     7921 cataatgctt acttacattt ataacttaca ttttttattc ctccattaat attcatggtt
     7981 tgctctttga ttcttattga gtttaaaaag ttgcgcctct ttttaacaac attatatcat
     8041 atatctaact taatgcaata tataaatgtc attttgtaaa attttattaa caaaaaactc
     8101 tctacaacaa aataatagag agttttctgc ttattaattt ttttcaaaat atctaaacat
     8161 cacttctttg tttaatttga taggctttga tacctaagct ccgactttat cacttaaaaa
     8221 ataaaaaagg acagctaatc tcttgcgcat caagggattt cgccattctt taatctcgtt
     8281 taagtgttgt atatggagct ttgcataatt ttttaaaact aaacaactac attaaacacg
     8341 aactgtgacg ctggttccat cttccttata aagattgatg agtccttctt ttaatgcacg
     8401 aaccatgtct cctgattgaa cgggttgagg caccttaata gtcaatagtt ccattggatt
     8461 tggagcgcgg tcgattttat tgcctttggc atcatgcaaa tcttcaatat aggtttcaaa
     8521 atgacggaaa cctggaccat aaaactcaac ttggtcccct tcgttaatga catttcgttg
     8581 acgaatagtt gctgtttgtg ccgcatcatc ataagaaacc acttcagcga caaacttgta
     8641 ctcaggaatt ttacgacgag caccaaacaa ctgctcattt tcagatggtg taccatagta
     8701 aaatcctgta gccagttcac gttgggcaac cttccacatc tcgtccacca agtcttgttt
     8761 gatagcttca aacttttcag gactttcaag ataggcatcc acagccgcct tgtagcagtt
     8821 ggttactgtt aatacatagt gaatagactc catacgtcct tcgattttta gactgtccac
     8881 accattttca atcatatctg gaatgtggtc aatcatagac atgtcaacgg ctgacattga
     8941 aaattcttct ggaatctcac cctgcaaact cttacgttct ttcccaaatg gcatatcgta
     9001 aaggtcgtac ttccaacggc atgactgaga acatccacca cggttggcat cacgcatact
     9061 ctagacggat tgttttgaca attctaatct tttagacttt gcataataac tgttaaacag
     9121 ttgaaaattg acccagtttt aacaagtagt caaaatacca gataaaggaa aaaagtcttt
     9181 aaaatcttaa aaacgcatag tatcaggtat tgaacaatct tgatattatg cgttttattg
     9241 tgggaagatt tacttcattt tctcttggaa ttgagttttc tcctacttca aaactagatg
     9301 atttgctatt taaaatagcg ttccttatga ttttggcaac ctggattaaa gcgagaaaaa
     9361 caaaaggggc aacttaagct ttctttatat tgcttatatg ttagtagttt tagacaaaca
     9421 ccacataaaa taggcttatc ctgtatcaaa gataagggat acggctcaaa agggtgattt
     9481 tctaatgaat catgacaccg ataacaagca taatactttt tacaatcata gcattgtaaa
     9541 gcaataatat ccagcttgct atggtaatga atgcatcttg tttgcttatc cactaagaaa
     9601 ccctgtgcct ctatcatttc cttttccttt cttaagctcg aactgtgacg ctggttccat
     9661 cttccttata aagattgatg agtccttctt ttaatgcacg aaccatgtct cctgattgaa
     9721 cgggttgagg caccttaata gtcaatagtt ccattggatt tggagcgcgg tcgattttat
     9781 tgcctttggc atcatgcaaa tcttcaatat aggtttcaaa atgacggaaa cctggaccat
     9841 aaaactcaac ttggtcccct tcgttaatga catttcgttg acgaattgtt gctgtttgtg
     9901 ccgcatcatc ataagaaacc acttcagcga caaacttgta ctcagggatt ttacggcgag
     9961 caccaaacaa ctgctcattt tcagatggta taccgtagta gaaacctgtt gccaattcac
    10021 gttgggcaac cttccacatc tcgtccacta agtcttgttt gatagcttca aacttttcaa
    10081 aactttcaag ataggcatcc acagccgcct tgtagcagtt ggttactgtt gatacgtagt
    10141 gaatagactt catacgtcct tcgattttta gactgtccac accattttca atcatatctg
    10201 gaatgtggtc aatcatagac atgtcaacgg ctgacattga aaattcttct ggaatctcac
    10261 cctgcaaact cttacgttct ttcccaaatg gcatatcgta aaggtcgtat ttccaacggc
    10321 atgactgaga acatccacca cggttggcat cacgcatact catgtggttt gaaagagtac
    10381 aacgtccaga gtatgaaata cacatagctc catggacaaa ggcttcaatt tcaacatctg
    10441 tacgtttgcg gatttcagct aattcttcca ttgaaacctc acgcgctaaa acgacacgag
    10501 tcaagcctag ctctttccag aactcaaggg tttcatagtt agtggcactg gcttgggtag
    10561 aaaggtggat ttcaaggcct ggtgcttcag tcgctgcaat cataatcaag gctgggtcag
    10621 atacgataac tgctgcaatc ccgatatcac gcagtttacg gaaccactca ccagcaccag
    10681 cttcatttcc ttcgtgcata accatattag ccgctacata gaccttggca ccatacttgg
    10741 ccgcaaactg cacgccttct tccatctgtt cgaaagtaaa gtttcccgca cggctacgaa
    10801 gaccataggc ctgaccaccg ataaagacag catctgctcc atactgaaca gctaccttta
    10861 gcttctctaa agtccctgca ggtgataaaa cctcaggacg ttttaatgtt tttgtcataa
    10921 tttctcctat ttgcaatata aaagtttgac atttttcctt ctcattttat agtaaataaa
    10981 ggataaaatc aagcctagac ggattgtttt gacaattcta atcttttaga ctttgcataa
    11041 taactattaa acagtcgaaa attaccccag ttttaacaag tagtcaaaaa gtctaaaaaa
    11101 gtaaaaacca gtatcccaag atactgactc gtaaaaatat ataaattagt ccttagatga
    11161 ctactcccac tctgttgctc ctataatgga aaaggattga tacagtctag aatatcgctt
    11221 ttttatttat tattatgata taaaactgtt agaattgatt ttttgtttta tacccaaata
    11281 tgaatagaga cgtagctaat aagttgaata acatatttgg aaaaatgaga aatagcaata
    11341 ttactagaac ttttagaaac aatcaatatt taaaaagaaa aaagctcaaa gaatttatta
    11401 aaccatgtta taatagatat agctataatt ttatttataa caagaggata gaaatgaccg
    11461 aattagaaag aaaaaatcga aaaattagct aagaaatatt ctgataactt aaacatcaaa
    11521 gttcaagaga gagttcgtga aatggcaaat gataataaga gccattattt gatatacaga
    11581 gttttaggta tttcatttga agaaggagaa aatatcgatt tgtatcaaaa taaaggtcgt
    11641 tttttataca aatatgctgg ttcattttta gaagaagctg cagtactatc ctttaacgaa
    11701 aaatttggta cagaaaatac ttaaaaagtt aacattccta attctgaaag tacaaaacct
    11761 aagacttttg aaattgattg tttagtcgga gaaaaacacg catacgaaat aaaatggtgg
    11821 gatgcaacta cagatggaga ccatataact aaagaacaca ctagaataaa agttattcat
    11881 aacaaaggat atataccaat tcggttaatg ttctactatc caaatagaac tcaagctata
    11941 aaaattcagc aaactttaga aacattgtat aacggtattg gagggaaata ttatggagat
    12001 tctgcctggg aacatttaag agcagtgacc ggtattgatt tacttagtat tctaacagat
    12061 attgcaaata aaaaacaggg gtaaaatcaa aatgacagta ttaaaaggag ataacttaga
    12121 aatattaaaa actattgaat cctcaagtat tgatttaatc tatatggacc ctcctttctt
    12181 tacacagaaa acccaaaaat tatctaataa caaaaatatt atgtattcat tcgaagatac
    12241 gtggacttcg attgaggatt acaaagaatt tttgtctgta agattagaag aatgcaaaag
    12301 agtgctaaaa aatagtggca gtattttcgt tcattgtgat aaaattgcaa atcatcatat
    12361 tagattaatt ttagataata tctttggagc agatatgttt caaagcgaaa ttatatggaa
    12421 ctataaacgg tggtctaatt caaaaaaggg attattgaac aatcatcaaa acatttactt
    12481 ttattcaaag tcaaaagatt ttaaatttaa tacaattttt acagagtatt cttctactac
    12541 aaatatcgac caaatactag tggaacgaaa acgagatgga aactctaaaa ctatatataa
    12601 ggttgataat aatggtaact atattctagc aaaagagaaa aatggagttc ccctttcaga
    12661 tgtttggaat ataccatttc ttaatccaaa agctaaagaa agagtaggtt atcctacaca
    12721 aaaacctatt ctgttattag aacaaattat aaagattgct actgataaaa atgatatagt
    12781 tttagacccg ttctgtggaa gtggaactac tttagtagcc tccaagattt tgaatagaaa
    12841 ttatatgggg attgatttat ctgaggaagc tatcaatata actcagcaac gtctggaaaa
    12901 tgttataaaa acaagttcaa atttattgaa taaaggaatc gaagcatata gaaccaaaac
    12961 tgaggaagag gaaaacattc ttaaattatt acaggcaaaa attgttcaaa gaaataaagg
    13021 aattgatggt tttttaccta aacattttca aaaaaaaccg atacctataa aaattcaaaa
    13081 aaataatgaa tgtctgaatg agagtatctc tttattacag aatgctataa actccaaaaa
    13141 acttgatttt ggagtagtta taaaaactca ttcagataat ttattatttg attttgatac
    13201 gattcctgaa aatataatag tagttgacca ttttgaactt acaatcgaaa aatggttaag
    13261 taaaagtcaa cagttactat agtttgcaaa ttatttacag aaagaacaaa aaatgctctg
    13321 aaacactgat ttcaaggcat tttttgttag tttaaaatta ctcccattct tcttttccaa
    13381 acatacaata tatccaaaac cattcaaaat actagattct attttttata atatcactaa
    13441 atccacctaa ttataggacg ttttcagatt tttagtcccg tcccagtccc agtaccggag
    13501 aaatattgtt ttaatataat atctcttttt gtcttctaag ctcttaaaag caaaagaaca
    13561 agtaaagagt caagacaagg ataaaaagtc catattaggg caaataaaaa gctttaagac
    13621 agatgacaaa tctaagtcaa ataagaaaga ccatagcaaa ggtgcagaga gataaatatt
    13681 ggcggtcttc ggactgcctt tattttttta tccatttttc aaatcaaatt tattcagact
    13741 atatatgcac atatacactt aaattcatat aaaaacatgg cttgtaaaaa attactttaa
    13801 tcacaataat cgcatttaaa attgtgatgt ttgcaagcta aattacggac ttcacttgga
    13861 agttttccct tgtatctttt ataatagata gaaaatttgc tggcagatga atatccaaca
    13921 gattctgcta tctcttttat aggtagttca gtgtttaaaa gaagagtttc agctacattc
    13981 attctttttc tttgagtgta ctctgtaatg ctttgacaat atttttcctt aaataaattt
    14041 tttaatttag taccactcat tttagatatt ttttcaagcg tgccttgatt tacattcgtt
    14101 gcaaaatgat catctaagaa tcttgctaca tcttcaagtg ctttatcatc atcaatttca
    14161 attttatatt tttttctatt taagtatgtg tcaattacta tacttatcca ttcatttgcc
    14221 tttgctttaa agaaaaaatc agcggcagga gcgtccatct tacaatttaa tatttccatt
    14281 gccactcttt ctaaggcctt tgtaagtatt atttgattcg gttgaagcaa ggttgaataa
    14341 aaagattctg gattaatgtt aatagatgct aaatgttttt ctattagctc ttttttaaaa
    14401 ccaatggaaa cagcaagata acaacaattc tcgtgtaata aaaaaacaaa atcatctttt
    14461 atattatcaa aatcaaaagt acataaagag tttgcggtaa tagtttgata cggattaaac
    14521 ttttctccgt ttgcactgac aatgtaactt gaataaattg aaacatagtc tgacatacta
    14581 taagtgctat tttgaactac ttcctctttg atataaaaat catgtatatc gataatgaag
    14641 ctgcctcctt cataaaacca atataggcct tctgcataag ttgaattctc ttttttccaa
    14701 caaaatgtat gacctgcact tgaatacttc tcattgtttt tacattcatc tactttcata
    14761 taatctttta caaaatcata atatgacata acacactatc ccttttagac aatattccaa
    14821 ttagccttat taattcaaaa ctattgtatt agtaattata acagatgtat aatagaaaag
    14881 caatgataga tattatcaat taagcgaatt tatatctaaa agggatatta aagaaaggag
    14941 atatgcttat gaagatttac aaaaaactat ttgcttatgt ccaagataag aaatatcttg
    15001 gggttttggc cataattttt tctgctatat ctgctctact tacagtatat ggatattatt
    15061 taatctacaa atttctagat aagttaataa ttaattcaaa tttatccggt gcagagagta
    15121 tagcattaaa atctgttatt acactaacaa gtggagcgat attttatttt gtctcaggaa
    15181 tgttttcaca catcttggga tttaggcttg aaacaaattt aagaaaaagg ggaattgatg
    15241 gcttagagaa agcaagtttt aggttctttg acttaaatcc atctggtcaa ataagaaaga
    15301 tcatagatga taatgcagca caaactcatc aggtggtagc acacatgatt cccgatagtt
    15361 ctcaggcaat aatcacaccc gtacttgtac ttgcacttgg ctttatagta agtataagag
    15421 ttggcataat tttgcttgct cttactataa ttggcggctt aattttaggg gcaatgatgg
    15481 gcgagcaaga atttatgaag atataccaag aatccctatc taaactaagt gctgaaactg
    15541 ttgagtacgt gagaggaatg caagttgtaa aaatatttaa agcaaatgta gagtctttta
    15601 aaagctttta taaggcgata aaagattact caaagtatgc ttatgattat tccctatctt
    15661 gtaaaaggcc ttatgttttg tatcaatggt tattttttgg actgattgca attttaatta
    15721 ttcctatagt ttattttatg actagcttag ctagcgcaaa ggtgatttta cttgagctta
    15781 tcatgatttt atttttatca ggagttctct ttgtttcatt catgagaatg atgtggtact
    15841 ccatgtatat ttctcaagga aattatgcag tagatacttt agaggcgctt tacgaagata
    15901 tgcaaaaaga caaattagtg catggtaata tcaataattt taaaaactat aatatagaat
    15961 ttgagaatgt tagctttgct tataatgata aagctgtcat tgaaaattta tcctttaatt
    16021 tagaagaagg aaagtcctac gcacttgtcg gttcatctgg atcaggcaaa tcaacagtag
    16081 caaaacttat atcaggtttt tataatgtta ataaaggaag cataaagata ggcgggatag
    16141 caataagtga atattctgac gaagccttaa ttaaagccat ttcctttgtt tttcaagatt
    16201 caaaattatt caagaagagc atttatgata atgtagcttt agctaataaa gatgcgacga
    16261 aagatgacgt tatgagagcc ttaaaattag caggatgcga tttaatatta gacaaattcc
    16321 cagaaagaga aaatacaatc ataggctcaa aaggtgttta tttatccggt ggagaaaaac
    16381 aaagaattgc aattgctaga gcaattttaa aggattccaa aattattatt atggatgaag
    16441 catcagcatc tattgaccca gataacgagt ttgaattgca aaaagctttt aaaaatctta
    16501 tgaaggataa aacagttatc atgattgcac acaggctatc tacaattaaa gaccttgatg
    16561 aaattattgt catggatagt ggaaaaatta tagaaagagg gtctgacaaa gaattaatgt
    16621 caaaagatac aaggtataag agcctgcaag agatgtttaa cagtgcgaat gaatggaggg
    16681 tttcaaatga aagagtttta taaaaaaaga tttgctctta cagatggagg agcaagaaat
    16741 ttaagtaaag caacactggc ttcatttttc gtttattgta taaacatgct tcctgccata
    16801 ttacttatga tttttgctca ggaagttttg gaaaatatgg gcaaaagcaa tggcttttat
    16861 atagtattct cagttttgac tttgatagca atgtatattt tgctttctat cgaatacgat
    16921 aaattatata acacaaccta tcaagaaagt gcagatttaa gaataaggac agcggagaat
    16981 ttatcaaaat tacctctatc ttacttttct aaacatgaca tttccgacat ttcacaaaca
    17041 atcatggctg atattgaagg catagagcat gcaatgagcc actcaatacc aaaggtgggc
    17101 ggcatggtac tgtttttccc attaatatct gtaatgatgc tagcgggcaa tgtcaagatg
    17161 ggtttagctg taattatccc atctatttta agctttatat ttataccttt atctaaaaaa
    17221 tatcaggtta atggacagaa tagatattat gatgtcttaa gaaaaaactc agaaagcttt
    17281 caagaaaata tcgaaatgca aatggagatt aaagcatata atttatcgaa ggatattaaa
    17341 gatgacttat ataaaaaaat ggaagatagt gagagagtac acttaaaggc ggaagtaact
    17401 acaattttaa ctttgtctat atcttcaata tttagcttta tatctcttgc tgttgtgata
    17461 tttgtcggcg taaatctaat tattaataaa gagataaatt ctctctacct tataggatat
    17521 ttactagctg ctatgaagat aaaagactct ttagatgcat ctaaagaggg cttgatggaa
    17581 atattttatt tatcgcccaa aatagaaaga ttaaaagaaa ttcaaaatca agatttacaa
    17641 gaaggcgatg actatagctt aaaaaaattt gatattgatc taaaagatgt tgagtttgcc
    17701 tacaataaag acgcaaaagt tttaaatggt gtaagtttta aagctaagca aggagaggtc
    17761 actgctttgg taggtgcaag tggctgcggt aaaacaacta tcttgaaact tatatcaaga
    17821 ctttatgatt atgacaaggg acaaatctta atcgatggca aagatataaa agaaatatca
    17881 acagaatctc tttttgataa ggtctctata gttttccaag atgtggttct ctttaatcaa
    17941 agcgttatgg aaaatattag aatcggaaag caagatgcaa gtgacgaaga ggttaaaaga
    18001 gcagcaaaac ttgcaaattg cacagatttt atagaaaaaa tggataaagg tttcgataca
    18061 gttattggtg agaacggagc tgagctatca ggaggagaaa gacaaagatt atcaatagcc
    18121 agagccttct taaaagatgc gccgatattg atcttagatg agataacagc aagccttgat
    18181 gttaacaacg agaaaaagat tcaagagtct ttaaataatt tagttaaaga taaaactgtt
    18241 gtaatcattt cacatagaat gaaatccata gaaaatgcag acaagatagt agttcttcaa
    18301 aacggaagag tagaaagcga aggtaagcat gaagagcttt tacaaaaatc aaaaatttac
    18361 aaaaatttaa tagaaaagac aaaaatggca gaagaattta tttattagga ggactacaat
    18421 ggataataaa aaattaaaag taaaagattt agtaagcatc ggtgtttttg gcgtaattta
    18481 ttttgccttc atgtttggag ttggtatgat gggcttgatt ccaatattgt tcttaatata
    18541 cccgacagta ttagccatag ttgcaggaac tgttgttatg ttatttatgg ctaaggttca
    18601 aaagccatgg gcactattta tatttggtat gatatcacca cttgtgatgt ttgcagctgg
    18661 tcatacctac gtagttgtgg ttttatcact tatagtaatg ataatagcag aattaattag
    18721 aaagattggt aattataatt catttaaata caatatgctt tcttatgcaa tcttcagcac
    18781 atggatatgt agctctttaa tgcaaatgct tttagcaaaa gaaaaatata tggagtggtc
    18841 tttgatgacg atgggaaaag attatgttga tgtgttagaa aagttaataa cttatcctca
    18901 tatggcttta gtagccttag gtgctttctt aggaggaatt cttggagcat atataggcaa
    18961 ggctctattg aaaaaacact tttcaaatgg attatattgt gtgggatact ttactccttg
    19021 cctaatttta tggtgctatc tgaattaaac cctatagtta agatgttttt gagtatacct
    19081 attgttatta gaatgtttat tttaccattt atggcagcaa gctttatgat aaagacctcg
    19141 gatgtaggcg caataatttc atcgatggat aagcttaaga tttcaaagaa tgtatccata
    19201 cctattgcgg ttatgtttag attcttccca tcttttaagg aggagaagaa aaacatcaaa
    19261 atggctatga gagtaagagg gataaatttt aaaaacccag tcaaatatct tgaatatgtt
    19321 tctgtgccac tactcattat atcatctaat atatcagatg acattgcaaa agcggcagaa
    19381 acaaaggcaa tagaaaatcc aattgccaag accagataca ttcgcgtaaa gatacagcta
    19441 attgattttg tttatgtttt agcggttgct ggacttattg tgggaggctt aatatggttg
    19501 aaataaaaaa tttaagtctt gattatggtg aagagcatat attagatgat atatcactat
    19561 ccatagccga gggagagtgc gtgctattta caggaaaaag tggaagtggt aagtcatctt
    19621 taataaattc aatcaatgga ctagctgtaa ggtatgataa cgcaaagaca aagggcgaaa
    19681 taattattga tggtaagaat ataaaaaatt tggaacttta tcaaatctca atgcttgttt
    19741 caactgtttt tcaaaatcct aagacatatt tttttaatgt caatacgaca ttagaattat
    19801 tattttattt ggaaaatatc ggtcttgcaa gagaagagat ggacaggcgt ttgaaggata
    19861 tgcttgagat attcccgata aaaaatcttt tgaacagaaa tatatttaat ctatccggcg
    19921 gtgaaaaaca aattctttgc attgcagctt cttatatagc aggtacaaag attatagtta
    19981 tggatgagtc ttcatcgaat ttagatatta aaagcataag tgttttggca aagatgctaa
    20041 agatattaaa agagaaaggc ataagcataa ttgttgcaga gcatagaatt tattatttga
    20101 tggacatagt tgaccgtgta tttttaatag ataaaggaaa gcttaaaaaa acttatacta
    20161 gaagtgaatt tttaaagcta gataaaaatg aattaaatgc tttaagttta agagataaaa
    20221 aattaagtaa attaaaagtt ccttatttaa aagaaggtgg agagtatcag ataaaaaatc
    20281 ttagctacaa atttactgat gatgagtgct taagcttaaa agatatttcg ttcaagcttg
    20341 ggaaaattta tggcataata ggatccaacg gacgaggaaa atcaacgctt ttaagatgtt
    20401 taataggtct tgagaaaaaa tcaaaagaag aaatttattt taagggagag aagctatcta
    20461 aaaaagaaag actcaaaaac tcttcacttg ttatgcaaga tgtaaatcat caattattca
    20521 cagatgaagt attcaacgag cttagattag gagtaaagaa ttttgatgaa gaaaaggcga
    20581 aaatcatttt aaaagattta ggcctggacg aatttattga aaggcatccg atgagtttat
    20641 caggagggca aaagcaaagg cttgcaatag catctgttat gtgcaagaat tctccatttg
    20701 tcttttttga cgaaccttca agtggtatgg attattccaa tatgataaaa atatctgaac
    20761 tgattaataa gtataaaacc atggataaaa taatttttat tgtttcccat gatatagaat
    20821 ttttaaatga agtggcagat gaaatttttg aattataaaa tagagtctgt taaacatgtc
    20881 tatagaactg gtaaattgtc taaaaattta aaagatacta acacatattg atatttatta
    20941 gaaaattaac ttcaggattt ttacagtatt tataaacttt ataatatgta tttatagtat
    21001 taataatttc catatcttta gggaaattct ttatttactt accataatta aagagatgag
    21061 agataaatat cggcagtctt cggactgcct ttattttttt acttaattta atgcagtaaa
    21121 tcatacgatt gaaaggaatc tgctttatat gctataatta ggtagttaat ctatacattt
    21181 agaaaggtaa aatatagaat tggagaatga tatgggattt tcatataata aattgtaacc
    21241 g
//
LOCUS       DBEROU010000041        20997 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.041, whole genome shotgun sequence.
ACCESSION   DBEROU010000041 DBEROU010000000
VERSION     DBEROU010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20997
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.041"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            119..616
                     /locus_tag="ACL8CL_001634"
     CDS             119..616
                     /locus_tag="ACL8CL_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HGS1330853.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTTWDFTM
                     FEEKS"
     gene            616..2271
                     /gene="dnaX"
                     /locus_tag="ACL8CL_001635"
     CDS             616..2271
                     /gene="dnaX"
                     /locus_tag="ACL8CL_001635"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HGS1330854.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNVGAVPKQVA
                     PALSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLAGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2299..2493
                     /locus_tag="ACL8CL_001636"
     CDS             2299..2493
                     /locus_tag="ACL8CL_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HGS1330855.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2643..3413
                     /gene="sufC"
                     /locus_tag="ACL8CL_001637"
     CDS             2643..3413
                     /gene="sufC"
                     /locus_tag="ACL8CL_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HGS1330856.1"
                     /translation="MSVLEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3441..4703
                     /gene="sufD"
                     /locus_tag="ACL8CL_001638"
     CDS             3441..4703
                     /gene="sufD"
                     /locus_tag="ACL8CL_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HGS1330857.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFY
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKISYLERLESRGEGSDKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4714..5940
                     /locus_tag="ACL8CL_001639"
     CDS             4714..5940
                     /locus_tag="ACL8CL_001639"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HGS1330858.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKAINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQSASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAIEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5927..6367
                     /gene="sufU"
                     /locus_tag="ACL8CL_001640"
     CDS             5927..6367
                     /gene="sufU"
                     /locus_tag="ACL8CL_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HGS1330859.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQDQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6417..7829
                     /gene="sufB"
                     /locus_tag="ACL8CL_001641"
     CDS             6417..7829
                     /gene="sufB"
                     /locus_tag="ACL8CL_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017769002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HGS1330860.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8060..9301)
                     /gene="pbp3"
                     /locus_tag="ACL8CL_001642"
     CDS             complement(8060..9301)
                     /gene="pbp3"
                     /locus_tag="ACL8CL_001642"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase PBP3"
                     /protein_id="HGS1330861.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKNLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9404..10513
                     /gene="cozE"
                     /locus_tag="ACL8CL_001643"
     CDS             9404..10513
                     /gene="cozE"
                     /locus_tag="ACL8CL_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell elongation protein CozE"
                     /protein_id="HGS1330862.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
     gene            11159..11506
                     /gene="ssrA"
                     /locus_tag="ACL8CL_001644"
     tmRNA           11159..11506
                     /gene="ssrA"
                     /locus_tag="ACL8CL_001644"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11678..11765)
                     /locus_tag="ACL8CL_001645"
     tRNA            complement(11678..11765)
                     /locus_tag="ACL8CL_001645"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11729..11731),aa:Ser,seq:gga)
     gene            11927..12667
                     /locus_tag="ACL8CL_001646"
     CDS             11927..12667
                     /locus_tag="ACL8CL_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1330863.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     AENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAVSLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            12664..13575
                     /gene="pfkB"
                     /locus_tag="ACL8CL_001647"
     CDS             12664..13575
                     /gene="pfkB"
                     /locus_tag="ACL8CL_001647"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HGS1330864.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVELVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAELIKETYGKVEVEKR"
     gene            13572..15524
                     /locus_tag="ACL8CL_001648"
     CDS             13572..15524
                     /locus_tag="ACL8CL_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-specific PTS transporter subunit EIIC"
                     /protein_id="HGS1330865.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAEMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLALSGDTEVYRAANGAK
                     AATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALSVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGSLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLVGAIVSGVVYGYLRKPQA"
     gene            15661..17913
                     /locus_tag="ACL8CL_001649"
     CDS             15661..17913
                     /locus_tag="ACL8CL_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HGS1330866.1"
                     /translation="MERKEAIQRMLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGS
                     LAYLAIFGLLIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGT
                     MAQVVTDLTGFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWS
                     VYDIAEFFSRGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPP
                     VDMETGEILTEEAVQNLPPIPEEKWVEPEIILSQAELKFPEQEDDSDDEDVQVDFSAK
                     EALKYKLPSLQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVT
                     KYEVKPAVGVRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFR
                     ELWEQSQTKAENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASI
                     LMKARPDQVKFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYEL
                     FAKVGVRNIAGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRL
                     GQKARAAGIHMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLL
                     GRGDMLFKPIDENHPVRLQGSFISDDDVERIVNFIKTQADADYDESFDPGEVSENEGE
                     FSDGDAGGDPLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEMAGVIGPAE
                     GTKPRKVLQQ"
     gene            complement(17956..18387)
                     /locus_tag="ACL8CL_001650"
     CDS             complement(17956..18387)
                     /locus_tag="ACL8CL_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6556 family protein"
                     /protein_id="HGS1330867.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTITTIGSILGLITAGITIMN
                     ALDNNNKNTKKEPTTSQTTTFVKEIQKESPQENTTPNKENNTSQEKTQQEETPKSSVK
                     EEKKEDQKTATQDSTTPATSKPATENEKQPNTPTSENKSNQ"
     gene            18525..19667
                     /locus_tag="ACL8CL_001651"
     CDS             18525..19667
                     /locus_tag="ACL8CL_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGS1330868.1"
                     /translation="MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKIDEIFFTSGGTEGDNWVIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRPDTILVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIHQALL
                     NYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            19676..20890
                     /gene="thiI"
                     /locus_tag="ACL8CL_001652"
     CDS             19676..20890
                     /gene="thiI"
                     /locus_tag="ACL8CL_001652"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HGS1330869.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
BASE COUNT         6161 a         3892 c         4651 g         6293 t
ORIGIN      
        1 gctccataat atctataagg gatttaccca ctacaaatat tatagagccc aaaatccttt
       61 gtttactaaa caagggattt ttcttttgtc tctgctcctt ttttgatata atagttctat
      121 gttaaaatca gaaaaacaat cacgttatca aatgttaaat gaagaattgt ccttcctatt
      181 ggaaggcgaa accaatgttt tggctaatct ttccaacgcc agtgctctta taaaatcacg
      241 ttttcctaat accgtatttg caggctttta tttgttcgat ggaaaggaat tggttttagg
      301 tcccttccaa ggaggtgttt cctgcatccg tattgcacta ggcaagggtg tttgtggtga
      361 ggcagctcac tttcaggaaa ctgttattgt tggagatgtg acgacctatc tcaactatat
      421 ttcttgtgat agtctagcta aaagtgaaat tgtggtgccg atgatgaaga atggtcagtt
      481 acttggagtt ctggatctgg attcttcaga gattgaggat tacgatgcta tggatcgaga
      541 ttatttggaa caatttgtcg ctattttgct tgaaaagaca acatgggact ttacgatgtt
      601 tgaggaaaaa tcttaatgta tcaagcactt tatcgaaaat atagaagtca aaacttctcc
      661 cagttagttg gtcaagaagt tgtggctaag actcttaaac aagcggtgga gcaagagaaa
      721 ataagtcacg cttatctttt ttctggtcct cgtggaacgg gaaaaaccag tgttgctaaa
      781 atctttgcca aggctatgaa ctgtcccaat caagtgggtg gcgaaccttg caataactgc
      841 tatatttgtc aagcagtgac ggacggtagt ttagaagatg tcattgaaat ggatgcagct
      901 tctaataatg gggtagatga aattcgcgaa attcgtgata aatctaccta tgcacctagc
      961 cttgctcgtt ataaggttta tatcatagat gaggttcaca tgctgtctac aggggctttt
     1021 aatgccctcc taaagacgct ggaagaacca acacagaatg tagtctttat tttggccact
     1081 actgaattgc acaagattcc tgctactatt ctatcccgtg tgcaacgttt tgagtttaaa
     1141 tcaattaaga cacaggatat taaggaacat attcactata tcttagaaaa agaaaatatc
     1201 agttctgaac cagaggctgt ggaaatcatt gccagacggg cggaaggtgg aatgcgggac
     1261 gccttgtcta ttttggatca agccctgagt ttgacacagg gaaatgagct gacgactgct
     1321 atctctgaag aaattactgg caccattagc ctatcagcct tggatgatta tgtggcggcc
     1381 ttgtctcaac aggatgttcc caaagctttg tcttgcttga atcttctttt tgacaatggt
     1441 aagagcatga ctcgttttgt gaccgatctt ttgcactatt taagagactt gttaattgtt
     1501 caaacagggg gagaaaatac tcatcatagt tcagtctttg tagaaaattt ggcacttcct
     1561 caaaaaaatc tgtttgaaat gattcgctta gcaacagtga gtttagcaga tattaagtct
     1621 agtttgcagc ccaagattta tgctgaaatg atgaccgtcc gtttggcgga aatcaagccc
     1681 gaaccagctc tatcaggagc ggttgaaaat gaaattgcta cgctgagaca ggaagttgcc
     1741 cgtctcaaac aagagctttc taatgtaggt gcggttccta aacaagttgc accagctctt
     1801 agtcgaccag ctacgggcaa aacagtctat cgtgtcgatc gcaataaagt gcaatctatc
     1861 ttacaagagg ccgtcgaaaa tcctgattta gcacgtcaaa atttaattcg tttgcagaat
     1921 gcctggggag aggtaattga aagtctaggt gggccggaca aggctctgct agctggttct
     1981 caaccggttg ctgccaatga acaccatgct attcttgctt ttgagtctaa cttcaatgct
     2041 ggtcaaacta tgaaacgaga caatctcaat accatgtttg gtaatatcct cagtcaggcg
     2101 gcaggttttt cacctgagat tttagctatt tccatggagg aatggaaaga agttcgcgca
     2161 gccttttcag ccaaagccaa atcttctcaa actgaaaaag aagtagaaga aagcctgatt
     2221 ccagaaggat ttgaattttt ggctgataaa gtgaaggtag aggaagacta aagaaagatt
     2281 tcatgataca ataagtttat gaataaacaa caatttatta ttatggcgct gtttacagct
     2341 gctgagacct attttttcaa tgaagcctgg atgactggcc gctatattat ggcagccttt
     2401 tgggcaattt tactctttag aaatttccga gtcagttatg tgatgggcaa aatcgttgat
     2461 gtcatcgatc agcattttaa taggaaagac tagccctcag cttccagaca aaatcaaagc
     2521 cttttaggct tttttttgtt atactagaaa agtatattta tagaattttt gctctatttc
     2581 tggggaaatc agacgttttt ctagtaagta ctgtaaaagt tttgaaaaag aaaggaacta
     2641 tcatgtcagt attagagatc aaagatcttc acgttgagat tgaaggaaaa gaaattttaa
     2701 aaggggttaa cctgaccctg aaaacaggag aaattgccgc tatcatggga ccaaatggta
     2761 caggtaaatc gactctttct gccgctatca tgggaaatcc aaactatgaa gtaactaaag
     2821 gtgaagtttt gtttgatggc gtaaacatcc ttgagttgga agtggatgag cgtgcgcgta
     2881 tgggactttt ccttgctatg caatacccat cagaaatccc tggaattacc aatgctgagt
     2941 ttcttcgtgc cgctatgaat gcgggtaaag aagatgatga gaagatttca gttcgtgagt
     3001 ttattactaa gctagatgaa aaaatggaat tgctcaacat gaaagaagaa atggcagagc
     3061 gttacctcaa cgaaggcttc tctggtggtg agaaaaaacg caatgaaatt cttcaacttt
     3121 tgatgttgga gccaacattt gctcttttgg acgagattga ctcaggtctt gatattgacg
     3181 ctcttaaagt tgtgtctaaa ggtgtcaatg ccatgcgtgg tgaaggtttt ggtgctatga
     3241 tcatcactca ctaccaacgt cttttgaact atatcacacc tgatgtggta cacgtgatga
     3301 tggaaggtcg tgttgtcctt tctggtggtc cagaattggc tgcgcgtttg gaacgtgaag
     3361 gatacgcaaa attagctgaa gaacttggct acgactacaa ggaagaattg taatttcctc
     3421 gtatctttta ggagaagtaa atgactagag aaaatattaa acttttttca gaaatgcacg
     3481 ctgaaccaag ctggttggct gatctccgtc aaaaagcttt tgacaagatt gagactttgg
     3541 aattaccagt tattgagcgt gtcaaattct accgttggaa tctgggtgat ggaacgatta
     3601 cagaaaatga gccatcagca aatgttccag atttcacagc tttagatcat cacttgaagt
     3661 tggtgcaagt aggaactcaa actgttttcg aacaaactcc agttgagtta gctgaacagg
     3721 gtgttgtctt cacagacttt cactcagctt tagaagaaat tccagagctg atcgaagaat
     3781 tcttcatgtc atctgttaag tatgatgatg acaagttggc ggcttaccac acagcttact
     3841 ttaacagtgg tgctgtactc tatattccag ataatgtaga aattacagag ccaattgaag
     3901 gaattttcta ccaagatagc gatagcaatg tgccgtttaa caagcatatt atgattatcg
     3961 ctggtaaaaa ttctaagatt agttatctgg agcgtttaga gtcacgcggt gaaggaagtg
     4021 acaaagcaac tgccaatatc acagtggaag tgattgcacg ttctggtgcg caagtcaagt
     4081 ttgctgctat cgaccgtcta ggtgaaaacg tcactgccta cattagccgt cgtggtaaat
     4141 taggcaacga tgcaagtatt gactgggcta tcggtgtcat gaacgaagga aatgtcgttg
     4201 ctgattttga tagtgacttg attggtaatg gtagccatgc tgacctcaag gttgtagctc
     4261 tttcaagtgg tcgtcaggta caagggattg atactcgtgt aactaactat ggctgcaact
     4321 caatcggaaa cattctacaa catggggtta tccttgaaaa agcaactttg actttcaatg
     4381 gtatcggcca catcatcaag ggtgctaagg gagcagatgc gcaacaagag agccgtgttc
     4441 tcatgctttc agaccaagcg cgttcagatg ctaacccaat tcttttgatt gatgaaaatg
     4501 acgtaactgc aggacatgca gcctctattg gtcaggtaga tccagaagac atgtattacc
     4561 tcatgagtcg tggattggat aaggcaactg cagagcgttt ggttgttcgt ggtttccttg
     4621 gatctgttat cgtggagatt ccagtcaagg aagttcgtga tgaaatgatt gcaactatcg
     4681 aagagaaatt gtcaaaacgc taaggggaag cctatgttag atgtagaagc gattcgcaag
     4741 gattttccaa ttttagatca gattgtcaat gatgaacctc tggtctatct ggacaatgct
     4801 gcgacgacac aaaaaccact agtagttctg aaagctatta acagctacta tgagcaggac
     4861 aatgccaatg ttcaccgtgg tgtccatacc ttagcggaac gagcgacagc ttcttatgaa
     4921 gctgctcgtg aaaccattcg taagtttatt aatgcaggct ctacaaagga agttctcttt
     4981 accagaggaa cgacaaccag ccttaactgg gtggcacgct ttgctgagga aattctcact
     5041 gagggagacc aggtcttgat ttcagtaatg gaacaccatt ctaatatcat tccatggcag
     5101 gaagcttgtc gaaagactgg agcagagctt gtctatgtct atcttaaaga cggtgccttg
     5161 gatatggagg atttgcgagc taaattgact gataaggtta aatttgtttc cctagctcat
     5221 gcctccaatg ttcttggtgt ggtcaatccg atcaaggaaa tcactcaatt agcccaccaa
     5281 gttggggcaa tcatggtagt ggatggtgct caatctacac ctcatatgaa gattgatgtc
     5341 caggacttgg atctggactt tttcgccttt tcgggtcaca agatggctgg tccgactggt
     5401 atcggtgtcc tttacggcaa agaaaagtat cttgagcaaa tgtctccagt agaatttggc
     5461 ggcgagatga ttgattttgt ctacgagcaa tctgctagtt ggaaggaatt gccttggaaa
     5521 tttgaggctg gaacgccaaa tatggcagga gctattggac ttgcgactgc agttgattat
     5581 ctggaaaaga ttggtatgga tgccattgaa gctcatgaac aggaattgat tgcgtacgtc
     5641 tatccaaaac tgcaggcaat tgagggattg accatttacg gttctcagga tttggctcaa
     5701 cgttcgggtg ttattgcctt taacctaggt gatctccatc ctcacgatct tgcgacggct
     5761 ctggattatg aaggagtggc tgttcgtgct ggtcaccatt gtgcgcaacc cttgcttcag
     5821 tatttggaag tcccagcaac agctcgtgca agtttttata tctacaatac caaggcagat
     5881 tgcgacaaac tagtcgatgc cctacaaaag acaaaggagt ttttcaatgg cactttctaa
     5941 actagatagc ctttatatgg cagtggtagc agaccattcg aaaaatccac atcaccaagg
     6001 gaagttagaa gacgctgagc aaatcagtct caacaatccg acttgtgggg atgtcatcaa
     6061 cctctctgtc aagtttgatg cagaggaccg tttggaagat attgcttttc taaattcagg
     6121 atgcacgatt tcaactgctt ctgctagtat gatgacagat gccgttttag gaaaaaccaa
     6181 acaagaaatt ttagaactgg cgactatttt ttctgaaatg gttcaagggc aaaaagatga
     6241 gcgtcaagac caacttggag acgcggcatt cttgtcaggt gttgccaaat tccctcaaag
     6301 aatcaagtgt gcaaccctag cttggaatgc ccttaagaaa acaattgaaa atcaagaaaa
     6361 acagtaagac aagtttcttt tgtcttatga attattagaa atgaagaaag gatattatgg
     6421 ctgaagaaag agtagaacca aaaccaattg accttggtga atataaattt ggtttccatg
     6481 acgatgtaga gcctgtctta tcgacaggaa aaggactcaa cgaaggtgtt attcgtgaat
     6541 tatctgctgc taagggtgag cctgagtgga tgttggagtt ccgtttgaag tcttatgaaa
     6601 ccttcaaaaa aatgcccatg caaacttggg gagcagactt gtcagagatt gactttgatg
     6661 acttaatcta ctaccaaaaa ccatctgaca aaccagcccg ttcttgggat gatgtacctg
     6721 aaaagattaa agaaaccttt gaacgtatcg ggattccaga agctgaacgt gcttatttag
     6781 caggggcttc tgcccagtac gagtcagaag tggtttacca caacatgaag gaagagttcc
     6841 aaaaattagg tattatcttt acagatacag attccgcact caaggaatac ccagacttat
     6901 ttaaacaata ctttgcgaag ttggtaccgc cgacagataa caagttggca gccctcaact
     6961 cagcagtatg gtcgggtgga acttttatct acgtgccaaa aggtgtcaag gtagatattc
     7021 cacttcaaac ttatttccgt atcaataacg aaaatatagg tcagttcgaa cgtaccttga
     7081 ttatcgttga tgagggagca agcgtccact acgtagaagg atgtacagca ccaacatatt
     7141 caagcaatag cttacacgct gccattgtag aaatttttgc tttggacgga gcttatatgc
     7201 gttatacaac tatccaaaac tggtctgata acgtctataa cttggtaaca aagcgtgcta
     7261 aggctcaaaa ggatgccact gttgagtgga ttgatggaaa cttgggtgcc aaaacgacta
     7321 tgaaatatcc atctgtttac cttgatggag aaggagcgcg tggtaccatg ctctctatcg
     7381 cctttgctaa tgcagggcaa caccaagaca cgggtgctaa gatgattcac aatgctccac
     7441 ataccagctc gtctattgtg tctaaatcca tcgctaaagg tggaggaaag gttgactacc
     7501 gtggacaagt cacctttaac aagaactcta agaaatctgt ttcccacatt gaatgtgata
     7561 ccattatcat ggatgacttg tcagcatcag atactattcc atttaatgaa attcacaact
     7621 cgcaagtggc tttggaacac gaagccaaag tatctaagat ttcagaagag caattgtatt
     7681 atctcatgag ccgtggattg tcagaatctg aggcaactga aatgattgtc atgggatttg
     7741 tagaaccctt tacaaaagaa cttccaatgg aatacgcagt tgagctgaac cgcttgatta
     7801 gctatgaaat ggagggatca gttggataaa atttgatttt atactcttcg aaaatctctt
     7861 caaaccacgt cagcatcgcc ttaccgtatg tatggttact gacttcgtca gtttcatcta
     7921 caacctcaaa acagtgtttt gagcaacctg cggctagctt cctagtttgt tctttgattt
     7981 tgagtattag atttactcaa aatcaaggat tttgaagatg aacttgtatc aaaaaatcgc
     8041 ggtttaaaat cgcgattttt tataatttct cgttaacaaa gcggacaaac tgattccacc
     8101 aaacttttaa gaagaaggct ttttcaattt tcttgtctgc taccatttcg aaactagggc
     8161 gctctgtggt gatgtaacct tgaccaatca agttcttgtc ttcataagtc aaatggccaa
     8221 ccactgttcc agcttcaagt ggtgctggga ttgctttgga atcaggtgtg aattgaacag
     8281 attgggaaga ttgattccca acacgttcta ttagatagat atcctctgga gccactgcag
     8341 ttactgtatc ttcttttcca tcttgtacag gggctttgct atcttgatag gcatcgcctt
     8401 gttgaacgat tttgcgaagt gtaaatgtag aagaaatata atccattagg gaagatgtag
     8461 ctgtaaatcg agcgtaagga ttattgtctt gatgatctgc atttaaaaca actgtgatga
     8521 ctctcatgcc tttttcgaca gtagtaccaa caaaagactc tccagcctta tctgttgttc
     8581 ctgtttttag cccatcaaaa ccaccacggt aagcaggcat accttctaac atgtagttgg
     8641 ttgaagtgat tgtcatccca gcaaaagtag aagaaggttt tttggtgatt tctaagactt
     8701 gtgggtattt tttgatgagg ttgcgagcaa cgatagcgac atcataagca ctaagcttat
     8761 tttcctcatc ttttttagaa cctgggtaaa tgttatcccc tagagtttca ttgttaagac
     8821 ctgtcgtatt gacaacagtg gcatcctgaa ttccccattc caagagtttt gcccgcatca
     8881 tatcgacgaa atctttttct gagccagcaa ttttctcagc tagggcaata gcggcgctgt
     8941 tggcactaga taccagagtt gcttcaagca actcttcgac agtataatta cgggcctcca
     9001 taggaatatt actggcttca gaatttgtcg tcaattgata aggataatca gaaatatcta
     9061 caggagtgga gagggtaata cttccgtttt ccaaagcttc atagaccaga taaacagtaa
     9121 tcaattttgt tatggaagca atttcgacag gttgcgttgc atccttctca tagagaattt
     9181 taccagtatt tgcctcaaca gcaatcgcat gtttagcggc aatggtaaaa tcttgagcaa
     9241 cagcagtaga agcaccccct aaaagagaga cagttaacaa agttaaaaat atttttttca
     9301 tagtagtttt attctatcat aaagaaaaaa aatattcttg ctttaataat tcatctgtta
     9361 agctttttga aaatatggta aaataaagtg agggaggtaa ctcatgtttc gtagaaataa
     9421 attatttttt tggaccacag aaattttact cttaaccatc atcttttacc tatggagaca
     9481 gatggggtct ttgattaacc cttttgttag cgtgcttaat acaattatga ttccattttt
     9541 attagggggc tttctttatt atttgacaaa ccctattgtt actttcttaa ataaagtctg
     9601 taaactcaat cgtttgcttg gtattttaat taccttgtgt actttggtct ggggaatggt
     9661 cataggtgtt gtctatctct tacctatttt gattaatcag ttatctagtt tgattatatc
     9721 tagtcaaact atttatagtc gagtacaaga cttaatcata gacttatcta attatcctgc
     9781 gctccagaat ttggatgtag aagctacaat tcagcagtta aacttatcct atgttgatat
     9841 tcttcaaaat atcctaaata gcgtatcaaa tagtgtgggg agcgtcttgt cagctcttat
     9901 cagtactgtt ttgattttga ttatgactcc agtttttttg gtttatttct tattagatgg
     9961 acataaattc ttgcccatgc ttgaaagaac gattctaaag agggatcgct tgcatattgc
    10021 aggcttatta aagaatttaa atgcgacgat tgctcgctat attagtggag tttcgattga
    10081 cgcaatcatt ataggttgtt tggcttatat tggctatagt attattggtt taaaatatgc
    10141 tttagttttt gccatttttt ctggtgtagc caatttaatt ccttatgtgg ggccaagtat
    10201 tggtttgatt cctatgatca tcgcaaatat attcactgat ccccatagac tgctgattgc
    10261 agtgatttat atgcttgttg ttcagcaggt agatggcaat atcttatatc ctcgaatcgt
    10321 aggaagtgtt atgaaggttc atccaatcac gattttagtt ttacttttgt tgtcaagcaa
    10381 tatctatggt gtagttggaa tgattgtcgc agtgccaacc tattctatct tgaaagaaat
    10441 ttctaagttc ttatcccgtt tgtatgaaaa tcataaaata atgaaagaac gagaaagaga
    10501 attagctaag taaaagtcag gagaaccctg atttttcttt actggaagtg gcctttagat
    10561 tagaagactg aaaataagtt aaagtcttaa actaattttc acagctaaga atagtagaag
    10621 ttaatctgat aaaaatcgaa aaaaccagtg gaattccgtg tcagggtaag ttccactggt
    10681 tttcatagtc tattaaagtt cgaatgaaac ctatttatag tatattgaaa ctagaatagt
    10741 acacctctaa ttctaaaaca ttgttagaaa tcgatttgac tgtcctaatc tattcgtcct
    10801 attcttattt cattttacta tattttggtg caataagtga aaagtagtcc gaataatata
    10861 aggattgatt ttatagtttt taaactcaaa tgaattaaaa taaagagagt acgaaaattc
    10921 tcatctgaaa gtattttaga ataattctct tcgtgaattt cttcaaaaca gatagcttca
    10981 tcttaggtat gtgttaattg gatttcgtaa gctctattta caatcgtaaa gttatgcact
    11041 gagaaagttg tagttagtct tccagtttgc taaatttttc gaatagtatc ttttttaggt
    11101 atgtgatttc tttttgcatt tttgagttag ataaggtata atggttttat tgtcttttgg
    11161 ggtcgttacg gattcgacag gcattatgag gcatattttg cgactcgtgt ggcgacgtaa
    11221 acgctcagtt aaatataact gcaaaaaata acacttctta cgctctagct gcttaaaaac
    11281 cagcaggcgt gacccgattt ggattgctcg tgttcaatga caggtcttat tattagcgag
    11341 atacgattaa gccttgtcta gcggcttgat aagagattga tagactcgca gtttctagac
    11401 ttgagttatg tgtcgagggg ctgttaaaat aatacataac ctatggttgt agacaaatat
    11461 gttggcaggt gtttggacgt gggttcgact cccaccggct ccattattcc tttgcattcc
    11521 ttggtaaaac gttgttaaat caacgttttt tatttttatc tttggtattc ctttgcattc
    11581 ttttgctaaa aagggagtca caaacagacc ctattttaaa aaaggataga aaaaaggata
    11641 caacatttgt cgcatcctaa aaataatctt ttttccacgg aagacatggg attcgaaccc
    11701 acgcacgcta ttacacgcct accgcgtttc caacacggcc tcttaagcct cttgagtaat
    11761 cttccaatac ttactcaaat agtctaccat aaaggctctt atcttgcaat aaaaattcta
    11821 gaaataagaa aaatgataga ttttgaaaga aaatgataaa aaatgcttga cttcaaaaga
    11881 aagtatgata gaatgaatag tgtaaacgat aacaggaggt gattcggtgt taaaaacaga
    11941 gcggaagcaa ctgattttag aggagttaaa tcaacatcat gtagtttctc tagaaaaatt
    12001 agttagtttg ctagaaacgt cagaatcaac ggttcgaaga gacttggatg agttggaagc
    12061 ggaaaacaag cttcgtcgtg tgcatggtgg agcagaactc ccccactcct tacaggaaga
    12121 agaaaccatt caagaaaaat ctgtcaaaaa ccttcaagaa aagaagttgc tggctcagaa
    12181 agcagtctct ctcattaaag aaaaagatgt catctttatc gatgctggaa caacaactgc
    12241 ttttttgatt catgaattgg tcaataagaa tgttacagtt gtgaccaact ccattcacca
    12301 tgccgctcag ttggttgaaa agcagattcc aactgtcatg gttggaggaa acgtcaagac
    12361 ggcgacagat gctagtatcg ggggcgttgc tcttaaccag attaaccaat tgcactttga
    12421 ccgtgccttt atcggaatga atggtgttga cgatggctat tatacgactc ctgatatgga
    12481 ggagggagct gtgaaaagag ctattttgga gaatgccaag cagacctacg tcttggtgga
    12541 ttcgtcaaaa attggacaaa cttgctttgc caaggtagcc ccactcaaac gcgctatcgt
    12601 tatcactagt caagggcatg agctcttgca ggttattaag gagaaaacgg aggtaataga
    12661 agtatgattt atacagtcac actcaatcca tccattgact atatcgttcg tttggaccaa
    12721 gtcaaagttg gtagtgtaaa tcgtatggac agtgatgata agtttgctgg tgggaaagga
    12781 atcaatgtca gccgtgtctt gaaacgtttg aatataccaa atacagcgac gggatttatc
    12841 ggtggcttta ctggtaaatt tatcacagat actttagcag aggaagaaat cgagacacgt
    12901 tttgtccagg tggcagaaga tactcgtatc aatgttaaaa ttaaagcaga ccaagaaaca
    12961 gaaatcaacg gaacgggtcc aactgttgaa ctggttcagc tagaagaatt gaaagctatt
    13021 ttatctagtc tgacagcaga agatacagtt gtctttgcag gttcaagtgc taaaaatcta
    13081 ggtaatgtta tctataagga tttgatttcc ttgacgcgcc agactggtgc gcaagtggtc
    13141 tgtgactttg aaggacagac cttaattgat agtttggact accagcctct tcttgtaaaa
    13201 ccaaacaatc atgaacttgg agcgattttt ggggttaaac tcgaaagttt agatgaaatt
    13261 gagaaatacg ctcgtgagtt actggctaag ggtgctcaaa atgttattat ctctatggct
    13321 ggggacggtg ctcttcttgt cacatctgag ggagcttact ttgctaaacc aatcaaagga
    13381 acagtcaaaa attcagttgg ggctggtgat tctatggttg ctggattcac aggtgaattt
    13441 gtcaaatcaa aagacgcagt agaagccttc aaatggggag tggcttgcgg aacggcaact
    13501 accttctcag atgacttggc aacggcagaa cttattaaag aaacatatgg aaaagttgag
    13561 gtagaaaaac gatgaaaatt caagacctat tgagaaaaga tgtcatgttg ctagatttgc
    13621 aggcaactga aaaaacagct gtcatcgacg agatgattaa aaatttgaca gaccacggtt
    13681 atgtaacaga ttttgaaaca tttaaagaag gaattttggc gcgtgaagct ttgacttcta
    13741 ctggtttggg tgatggaatc gcaatgcctc acagcaaaaa cgctgctgtc aaagaagcga
    13801 cagttctatt tgctaagtca aataagggtg ttgactacga gagcttggat ggacaagcaa
    13861 ctgacctctt cttcatgatt gcagctccag aaggtgccaa tgatactcac ttggcagcct
    13921 tggcagaatt gtctcaatac ttgatgaaag acggttttgc agacaaactt cgtcaagcaa
    13981 catctgcaga ccaagttatc gaactttttg accaagcttc agaaaaaact gaggaacttg
    14041 ttcaagcacc tgctaatgac tctggtgact ttatcgtagc tgttacagct tgtacaacag
    14101 gtattgccca cacttacatg gcccaagaag cccttcaaaa agtagctgct gaaatggggg
    14161 ttggtatcaa ggtcgaaacc aacggtgcta gcggtgttgg aaatcaacta actgcagaag
    14221 atatccgtaa ggctaaagct attatcattg cagcagacaa ggccgttgaa atggatcgat
    14281 ttgatggaaa accattgatc aatcgtccag ttgctgacgg tatccgtaag acagaagagc
    14341 taattaactt ggctctttca ggagatactg aagtctaccg tgccgctaat ggtgcaaaag
    14401 ctgcaacagc ctctaacgaa aaacaaagcc ttggtggtgc cttctacaaa cacttgatga
    14461 gtggtgtatc tcaaatgtta ccattcgtta tcggtggtgg tatcatgatt gcccttgcct
    14521 tcttgattga cggtgctttg agtgttccaa atgaaaacct tggcaatctt ggttcttacc
    14581 atgagttagc ttctatgttc atgaaaattg gtggagctgc ctttggtttg atgcttccag
    14641 tctttgcggg ttatgttgcc tactctattg ctgaaaaacc aggtttggta gcaggtttcg
    14701 tggctggtgc tattgccaaa gaaggttttg cctttggtaa aattccttat gccgcaggtg
    14761 gtgaagcaac ttcaactctt gcaggtgtct catctggttt cctaggtgcc cttgttggtg
    14821 gatttatcgc aggtgccttg gttcttgcca tcaagaaata cgttaaagtt cctcgttcac
    14881 tcgaaggtgc taaatcaatc cttctattgc cacttcttgg aacaatcttg acaggatttg
    14941 ttatgttagc tgtgaatatc ccaatggctg caatcaacac tgctatgaat gacttcctag
    15001 gcagtcttgg aggaggttca gctgtccttc ttggtatcgt ccttggtgga atgatggctg
    15061 ttgacatggg tggaccagtt aataaagcag cttatgtctt tggtacaggt acgcttgcag
    15121 caactgtttc ttcaggtggt tctgtagcca tggcagcagt tatggctgga ggaatggtgc
    15181 caccacttgc aatatttgtc gcaactcttc ttttcaaaga taaatttact aaggaagaac
    15241 gtaactctgg tttgacaaac atcatcatgg gcttgtcatt tatcactgag ggagcgattc
    15301 catttggtgc cgctgaccca gctcgtgcga ttccaagctt catccttggt tcagcagtag
    15361 caggtggact cgttggtctt actggtatca aactcatggc gccacacgga ggaatcttcg
    15421 ttatcgccct tacttcaaat gctctccttt acctcgtttc tgtcttggta ggagcaatcg
    15481 taagtggtgt ggtttatggt tacctacgca aaccacaagc ataaaaaata gaaaaatgaa
    15541 aagattggac cgtttggtgc agtctttttc tcttcccgaa atgcctgtga aatatggtat
    15601 aatagaagaa tggcaaacaa gaatacaagt acaacaagac ggagaccgtc taaagcagaa
    15661 ctggaaagaa aagaagcgat tcaacgaatg ttgatttcgt taggaattgc gattttattg
    15721 attttcgcag ccttcaaatt aggggctgca ggtataaccc tttataattt aattcgcttg
    15781 ctagtgggta gcctagctta tctggcgata ttcggcctat taatctatct cttctttttc
    15841 aagtggatac gaaaacagga aggactctta tctggctttt tcaccatatt tgctggctta
    15901 ctcttgattt ttgaggccta cttggtttgg aaatatggtt tggacaagtc cgttctaaaa
    15961 gggaccatgg ctcaggttgt gacagatctg actggttttc gaacgactag ctttgctgga
    16021 gggggcttga tcggggtcgc tctttatatt ccaacagcct ttctcttttc aaatatcgga
    16081 acttacttta ttggttctat cttgatttta gtgggttctc tcctagtcag cccttggtct
    16141 gtttacgata ttgctgaatt tttcagtaga ggctttgcca aatggtggga agggcacgag
    16201 cgtcgaaaag aggaacgctt tgtcaaacaa gaagaaaaag ctcgccaaaa ggctgagaaa
    16261 gaggctagat tagaacaaga agagactgaa aaagccttac tcgatttgcc tcctgttgat
    16321 atggaaacgg gtgaaattct gacagaggaa gctgttcaaa atcttccacc tattccagaa
    16381 gaaaagtggg tggaaccaga aatcatcctg tctcaagctg aacttaaatt ccctgaacag
    16441 gaagatgact cagatgacga agatgttcag gtcgattttt cagccaaaga agcccttaaa
    16501 tacaaacttc caagcttaca actctttgca ccagataaac caaaagatca gtctaaagag
    16561 aagaaaattg tcagagaaaa tatcaaaatc ttagaagcaa cctttgctag ctttggtatt
    16621 aaggtaacag ttgaacgggc cgaaattggg ccatcagtga ccaagtatga agtcaagccg
    16681 gctgttggtg taagggtcaa ccgcatttcc aatctatcag atgacctcgc tctagccttg
    16741 gctgccaaag atgtccggat tgaagcacca atccctggga aatccctaat cggaattgaa
    16801 gtgcccaact ccgatattgc cacggtatct ttccgagaac tatgggaaca atcgcaaacg
    16861 aaagcagaaa atttcttgga aattccttta gggaaggctg ttaatggaac cgcaagagct
    16921 tttgaccttt ctaaaatgcc ccacttgcta gttgcaggtt caacgggttc agggaagtca
    16981 gtagcagtta acggcattat tgctagcatt ctcatgaagg cgagaccaga tcaagttaaa
    17041 tttatgatgg tcgatcccaa gatggttgag ttatctgttt acaatgatat tccccacctc
    17101 ttgattccag tcgtgaccaa tccacgcaaa gccagcaagg ctctgcaaaa ggttgtggat
    17161 gaaatggaaa accgttatga actctttgcc aaggtgggag ttcggaatat tgcaggtttt
    17221 aatgccaagg tagaagagtt caattcccag tctgagtaca agcaaattcc gctaccattc
    17281 attgtcgtga ttgtggatga gttggctgac ctcatgatgg tggccagcaa ggaagtggaa
    17341 gatgctatca tccgtcttgg gcagaaggcg cgtgctgcag gtatccacat gattcttgca
    17401 actcagcgtc catctgttga tgtcatctct ggtttgatta aggccaatgt tccatctcgt
    17461 gtagcatttg cggtttcatc aggaacagac tcccgtacga ttttggatga aaatggagca
    17521 gaaaaacttc ttggtcgagg agacatgctc tttaaaccga ttgatgaaaa tcatccagtt
    17581 cgtctccaag gctcctttat ctcggatgac gatgttgagc gcattgtgaa cttcatcaag
    17641 actcaggcag atgcagacta cgatgagagt tttgatccag gtgaggtttc tgaaaatgaa
    17701 ggagaatttt cggatggaga tgctggtggt gatccgcttt ttgaagaagc taagtctttg
    17761 gttatcgaaa cacagaaagc cagtgcatcc atgattcagc gtcgtttgtc agttggattt
    17821 aaccgtgcga cccgtctcat ggaagagctt gagatggcag gtgtcattgg tccagctgaa
    17881 ggtaccaagc caagaaaagt attgcagcaa taaaaaatag cttctttcca aggttggaag
    17941 gagctatttt actggctatt gattactttt attttctgaa gttggagtat tgggctgttt
    18001 ttcattttca gtggcaggtt tacttgtagc aggtgtagta gagtcctgag ttgctgtttt
    18061 ctgatcttct tttttctctt ccttgacgct agattttggc gtttcttctt gttgtgtttt
    18121 ttcttgagaa gtgttatttt ccttatttgg agtagtattt tcctgagggg attctttttg
    18181 aatttctttg acaaaggttg tcgtctggct tgtcgtaggt tcttttttag tatttttatt
    18241 attattatcc aaggcgttca tgatcgtgat acccgctgta atcaaaccaa ggatactacc
    18301 gatagtagta atggtttttt gaaaaacagt aaagcctttt ttgatatgtt ctgttttcgg
    18361 ttttgaagtt ctacgatagt tagacataca ttctctcctt tgattatgat ttattatact
    18421 ctgtttcttt aaaaaaatct taaaaaaaga gaaattgccc tttcttgtcg caagctccgt
    18481 ttcatgatac aatagaggga gtgattttag aaagcgtgag aaacatgatt tactttgata
    18541 attcagcgac gaccaagcct tatcctgaag cccttgaaac ctatatgcag gtcgcttcaa
    18601 aaattttagg aaacccatct agtctccatc gtttgggaga ccaagcaaca cgaatcttag
    18661 atgcttctcg ccaacagatt gcagatttaa tcggtaagaa aatcgatgaa atcttcttta
    18721 cctctggtgg aacagaaggg gataactggg ttatcaaggg tgtggccttt gaaaaagctc
    18781 agtttggcaa gcacatcatt gtttcagcca ttgaacatcc agcagtcaaa gagtcagccc
    18841 tctggttgaa aagtcaagga tttgaagtgg attttgctcc agttgataag aaaggcttgg
    18901 tcgatgttga ggcgttagca ggtttgatac ggcctgatac aatcctcgtt tccatcatgg
    18961 ctgtgaacaa tgaaatcggc tctatccaac ctattgaggc tatttcagaa ttcttggcag
    19021 acaagccgac tatttccttc cacgttgatg cggttcaggc gcttgccaaa attccgactg
    19081 aaaagtatct gacagaacgg gtggattgcg cgaccttctc gagtcataaa tttcatggtg
    19141 tccgtggtgt tggctttgtc tatatcaaat ctggcaagaa gattacacct cttcttacag
    19201 gtggtggcca ggagcgagat tatcgttcga caactgaaaa tgtggcaggg attgcagcga
    19261 cagccaaggc tctccgttta tctatggaaa agctagatat ctttaggagc aagactgggc
    19321 agatgaaggc agtgattcac caagctcttc tgaactatcc ggatattttt gtcttttcag
    19381 atgaggaaga ctttgcacct catattctga cttttggaat caaaggtgtt cgaggtgaag
    19441 tcatcgttca cgcctttgaa gactatgata ttttcatctc aacaacctca gcttgttcat
    19501 ctaaggcagg aaaaccagcc ggtaccttga ttgccatggg agtggacaaa gataaggcca
    19561 agtcagctgt gcgtcttagc ctagacttgg aaaatgatat gagtcaggtc gagcagtttt
    19621 tgaccaagtt aaaattgatt tacaatcaaa ctagaaaagt aagataggag cattcatgca
    19681 gtattcagaa attatgattc gctacggaga gttgtcaacc aagggtaaaa accgtatgcg
    19741 tttcatcaat aaacttcgta ataatatttc ggacgttttg tctatctata cccaagttaa
    19801 ggtaacagca gatcgcgacc gtgcccacgc ttacctcaat ggagctgatt acacagcagt
    19861 tgcagaatct ctcaaacaag tttttggaat tcaaaacttt tctcctgttt ataaggttga
    19921 aaaatctgta gaagttttga agtcttctgt ccaagagatt atgcgggaca tctacaagga
    19981 aggtatgacc tttaagattt ctagcaagcg tagcgaccac aactttgaac ttgatagtcg
    20041 tgaactcaac caaacacttg gaggggctgt attcgaagcc attccaaatg tgcaagttca
    20101 aatgaaaagt cctgacatca atcttcaggt ggagattcgt gaagaagcag cctatctttc
    20161 ttatgaaacc attcgtgggg ctggtggttt gccagttgga acttcaggta aagggatgct
    20221 catgttgtca ggagggattg actcacctgt agcaggttat cttgctctta agcgtggggt
    20281 ggatatcgag gcagttcact ttgctagtcc accatatact agtcctggtg ccctcaagaa
    20341 agcgcaggac ttgacccgta aattgaccaa gtttggcgga aatatccagt ttatagaggt
    20401 gcctttcaca gagattcaag aggaaatcaa agccaaagcg ccagaagctt atttgatgac
    20461 tctaactcgt cgctttatga tgcggattac tgaccgtatt cgtgaggtac gaaatggttt
    20521 ggttatcatc aatggggaaa gtctaggtca agtagccagc caaacccttg aaagtatgaa
    20581 ggctatcaat gctgttacca acactcccat cattcgtcct gtggttacca tggacaagtt
    20641 ggaaatcatt gacatcgccc aggaaatcga tacctttgac atttcaatcc aaccgtttga
    20701 agactgttgt accatttttg caccagatcg tccaaaaaca aatcctaaaa ttaagaatgc
    20761 ggagcagtac gaagcgcgta tggatgttga aggcttggtt gagcgagcag tggctggaat
    20821 catgattact gaaatcacac ctcaagccga aaaagatgaa gttgatgact tgattgataa
    20881 tctactctaa ttcagaaaat ccaaaagaat agcgaaaatc agtaaaaaaa gttagttttt
    20941 tctctaaaaa caggtaaaaa actaactttt tttattttta tgatataatg atataaa
//
LOCUS       DBEROU010000042        20350 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.042, whole genome shotgun sequence.
ACCESSION   DBEROU010000042 DBEROU010000000
VERSION     DBEROU010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 20350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20350
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.042"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            59..637
                     /locus_tag="ACL8CL_001653"
                     /pseudo
     CDS             59..637
                     /locus_tag="ACL8CL_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558202.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(736..2358)
                     /gene="groL"
                     /locus_tag="ACL8CL_001654"
     CDS             complement(736..2358)
                     /gene="groL"
                     /locus_tag="ACL8CL_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HGS1330870.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEVTGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAELGIGFNAATGEWVNMIDQGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            complement(2374..2658)
                     /gene="groES"
                     /locus_tag="ACL8CL_001655"
     CDS             complement(2374..2658)
                     /gene="groES"
                     /locus_tag="ACL8CL_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HGS1330871.1"
                     /translation="MLKPLGDRVLLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            complement(2814..3209)
                     /locus_tag="ACL8CL_001656"
     CDS             complement(2814..3209)
                     /locus_tag="ACL8CL_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HGS1330872.1"
                     /translation="MYNKVIMIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            complement(3287..4048)
                     /locus_tag="ACL8CL_001657"
     CDS             complement(3287..4048)
                     /locus_tag="ACL8CL_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HGS1330873.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEALKA
                     EFAETFPNQTVWTFLLDVTDMTIVKTVCSDILETIGQIDILVNNAGLALGLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            complement(4081..4707)
                     /gene="ytpR"
                     /locus_tag="ACL8CL_001658"
     CDS             complement(4081..4707)
                     /gene="ytpR"
                     /locus_tag="ACL8CL_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtpR family tRNA-binding protein"
                     /protein_id="HGS1330874.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNMERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            complement(4723..5040)
                     /locus_tag="ACL8CL_001659"
     CDS             complement(4723..5040)
                     /locus_tag="ACL8CL_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HGS1330875.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            complement(5037..5336)
                     /locus_tag="ACL8CL_001660"
     CDS             complement(5037..5336)
                     /locus_tag="ACL8CL_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HGS1330876.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDRKLMKTQEELTE
                     IVRDHFSDMGEIATFYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            complement(5534..5731)
                     /locus_tag="ACL8CL_001661"
                     /pseudo
     CDS             complement(5534..5731)
                     /locus_tag="ACL8CL_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724444.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
     gene            complement(5973..6647)
                     /locus_tag="ACL8CL_001662"
     CDS             complement(5973..6647)
                     /locus_tag="ACL8CL_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330877.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            complement(6653..7099)
                     /locus_tag="ACL8CL_001663"
     CDS             complement(6653..7099)
                     /locus_tag="ACL8CL_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS1330878.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            complement(7213..7716)
                     /locus_tag="ACL8CL_001664"
     CDS             complement(7213..7716)
                     /locus_tag="ACL8CL_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HGS1330879.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            complement(7713..8075)
                     /locus_tag="ACL8CL_001665"
     CDS             complement(7713..8075)
                     /locus_tag="ACL8CL_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HGS1330880.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            complement(8141..9172)
                     /locus_tag="ACL8CL_001666"
                     /pseudo
     CDS             complement(8141..9172)
                     /locus_tag="ACL8CL_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155117.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(9179..10000)
                     /locus_tag="ACL8CL_001667"
     CDS             complement(9179..10000)
                     /locus_tag="ACL8CL_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1330881.1"
                     /translation="MILLAILFTCFSVYLELEVPTYISKITDLLGSQGTNLDELWQPA
                     SMMMGMLFLAFLSVVAVGFFASRVAASYTSRLRSDIFNRVLDYSQTEIKKFSIPSLLT
                     RTTNDITQVQMLITMGLQVVTRGPIMAIWAIGKILGHSEYWLWAVLVAVIVNVLMTTV
                     LMTLDFPKQSLIQGLIDKLNSITRESLTGIRVVRAYNAEDYQNEKFAAVNDELTRLNL
                     FVNRLMAILNPIMMGISSGLSVAIYWIGAYVINDAAPIARLPLFSDMIVFMSYAM"
     gene            complement(10016..10487)
                     /locus_tag="ACL8CL_001668"
                     /pseudo
     CDS             complement(10016..10487)
                     /locus_tag="ACL8CL_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
     gene            complement(10611..11327)
                     /locus_tag="ACL8CL_001669"
     CDS             complement(10611..11327)
                     /locus_tag="ACL8CL_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1330882.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKAKGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            complement(12208..13623)
                     /gene="ply"
                     /locus_tag="ACL8CL_001670"
     CDS             complement(12208..13623)
                     /gene="ply"
                     /locus_tag="ACL8CL_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HGS1330883.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            complement(13634..14044)
                     /locus_tag="ACL8CL_001671"
     CDS             complement(13634..14044)
                     /locus_tag="ACL8CL_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330884.1"
                     /translation="MIKRGDVVALYLPFPTISSDLAVKNHMYICIDNSMTKNKELVKN
                     QTFKPALLTRRLVKNFMIEEPDLARNPFTRPTLIDLDKVFMLDNTVIPTSYLARRRRN
                     VSEELYEEILDHLVQPRLISLNKSEFMQLNPGTY"
     gene            complement(14037..14645)
                     /locus_tag="ACL8CL_001672"
     CDS             complement(14037..14645)
                     /locus_tag="ACL8CL_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330885.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            complement(14658..15116)
                     /locus_tag="ACL8CL_001673"
     CDS             complement(14658..15116)
                     /locus_tag="ACL8CL_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HGS1330886.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            15241..15640
                     /locus_tag="ACL8CL_001674"
                     /pseudo
     CDS             15241..15640
                     /locus_tag="ACL8CL_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(16404..16664)
                     /locus_tag="ACL8CL_001675"
     CDS             complement(16404..16664)
                     /locus_tag="ACL8CL_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330887.1"
                     /translation="MRERSATGAQDLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNDDISLDQNEIFEILKNFLDG"
     gene            complement(16723..17274)
                     /locus_tag="ACL8CL_001676"
     CDS             complement(16723..17274)
                     /locus_tag="ACL8CL_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330888.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFHSMIELFSIL
                     PEYPLKKDGREIPLYFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCGLYSSKI
                     SSNHVSFHLQPQNSVLSSLQLAS"
     gene            complement(17264..17461)
                     /locus_tag="ACL8CL_001677"
     CDS             complement(17264..17461)
                     /locus_tag="ACL8CL_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330889.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            complement(17465..18259)
                     /locus_tag="ACL8CL_001678"
     CDS             complement(17465..18259)
                     /locus_tag="ACL8CL_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330890.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEIPKELTPSEQLTWIAFLLTKGQKVVNVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENTSYVIWQRKFNQLSLPLSQHVLK"
     gene            complement(18667..19092)
                     /locus_tag="ACL8CL_001679"
     CDS             complement(18667..19092)
                     /locus_tag="ACL8CL_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330891.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVTKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEATSIFLA
                     KKHNGILGSNNLRDVKPYVEEFSLEHMTTGDILIEAFKA"
     gene            complement(19221..19580)
                     /locus_tag="ACL8CL_001680"
     CDS             complement(19221..19580)
                     /locus_tag="ACL8CL_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HGS1330892.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMALG"
     gene            complement(19626..19832)
                     /locus_tag="ACL8CL_001681"
     CDS             complement(19626..19832)
                     /locus_tag="ACL8CL_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330893.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            complement(19819..19980)
                     /locus_tag="ACL8CL_001682"
     CDS             complement(19819..19980)
                     /locus_tag="ACL8CL_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1330894.1"
                     /translation="MYVINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
BASE COUNT         6100 a         4533 c         3471 g         6246 t
ORIGIN      
        1 gctttcaaat ttctaggctc aggctgaaac agtctcccag actgtttcac tcccgaatgc
       61 taaaatcgtt cttgatcgct ttcacattgt acaacatctt agccgtgcta tgagtcgtgt
      121 gcgtgttcaa attatgaacc aattggatcg aaaatcccat gaatacaagg ctatcaagcg
      181 ctactggaag ctcatacaac aggatagccg taaactgagt gataagcgat tttatcgtcc
      241 tacttttcgt atgcatttaa ccaataaaga gattttagac aagcttttga gctattcaca
      301 agacttgaaa catcactatc agctctatca actcttgctg tttcactttc agaataagga
      361 accggagaaa tttttcggac ttattgagga caatcttaag caagttcatc ctatttttca
      421 gactgtcttt aaaaccttcc tcaaagataa ggaaaagatt atcaacgccc ttcaactaca
      481 ctattctaac gccaaaccgg aagcgaccaa taatctcatc aaacttatca agcgcaatgc
      541 ctttggtttt cgaaactttg aaaacttcaa aaaacggatt tttatcgctt tgaacatcaa
      601 aaaagaaagg acgaaatttg tcctttctcg agcttagctg acttcaaccc actacagttg
      661 acaaagagcc tataaaaaga agggttagtc attccctcct tttgtgtttt ttataagttg
      721 ttttctatag aaagcttaca tcatcccgcc catcatgctt ggatccattg ctggagctgg
      781 ggctactggt tctggtttat tggctacgac tgcttctgtt gtcaaaatca agctggctac
      841 agatgctgca ttttgtaggg ctgaacgact cactttaact ggatcaatga taccttgatc
      901 aatcatgtta acccactcgc cagttgctgc gttgaatcct ataccaagct cagcattttt
      961 caaacgatcg ataacgatag atccttcaaa tcctgcattg tgagcaattt gacgaacagg
     1021 ttcttccaaa gcacggagaa caatattacg tcctgttact tcatctcctg tcaattccaa
     1081 ggtagcaaca gctggaatca cattggcaag agctgttcca ccacctgcaa caataccttc
     1141 ttcaacagct gcacgagtag cgttgagggc atcttcaatg cggagtttca tttctttcaa
     1201 ctcagtttca gttgcggctc cgaccttaat aaccgctaca ccacctgaca atttggccaa
     1261 gcgttcttgc aatttttcac ggtcaaattc agaagttgta gtttcgattt gagacttgat
     1321 aaccgcaaca cggtgagaaa tcgcttcagg atttcctgca ccttctacaa taaccgtgct
     1381 atctttgtcc acggtcactc tcgctgcttg accaagagct tcaattgtcg catctttcaa
     1441 ctcaagacca aggtcttctg tgataactgt tccgcctgtt aagatggcga tatcttcaag
     1501 catggctttg cgacggtcac caaaaccagg tgccttgact gctactacgt tgaaggttcc
     1561 acgaatcttg ttcaaaacaa gagttggaag agcctcacca tccacatcat ccgcaataat
     1621 caagagtgga cgattgcttt ggagaatgct ttccaaaagt ggcaagattt cttggatatt
     1681 ggaaattttc ttgtctgtaa tcaaaatgta cggattttca aggtcagcca ccattttttc
     1741 gctatctgtc accatgtact gtgaaaggta accacggtca aactgcattc cttctacgac
     1801 ttcaagctct gtttccatac cacgtgactc ttcgatggtg atgacaccgt ctttgccaac
     1861 tttttccatt gcttcagaga tgtactcacc aactttttca gaacgagaag atacggctgc
     1921 aacttgagcg atagcttctt tattggcaac agggatggcg ttgtttttca aagcttcaac
     1981 tgctgcggca actgctgttt caatcccacg acgaataccg attggatttg cacctgctgt
     2041 gacgtttttg attccttcac ggacgatagc ttgggtcaag actgttgcag tcgtagttcc
     2101 gtcacctgcg atatcattgg ttttagaagc tacttctgat actaacttag cacccatatt
     2161 ttcaaaatgg tcttccaatt cgatttcttt ggcaatggtc acaccgtcat tggtaatcaa
     2221 gggtgaaccg aatgactttt caagaacgac attgcgacct tttggtccca aggttacttt
     2281 aacagtgtct gcaaggatat cgacaccacg aaccatggct gaacgggcat ctgatgaaaa
     2341 tttaatttct tttgacatac ttactttctc cttctattcc tcaatgattg ccaaaatgtt
     2401 agcttcgcct acgatgatgt acttttcatc gccatctttg acatcaagac ctgcgtgggc
     2461 ttcaactaag acacgatctc cagttttaac acttggagca accaagtcac cgttcaaggt
     2521 acgaacacct tgtccagtag ccacaacttg agctgttttg gttttttctt gggctgagcc
     2581 tgcaaggaca aagcccccaa cggtttgttc tttttcttct atttttaaga gcacacggtc
     2641 ccctaatggt ttcaacatct gatttcctcc ataatgagat agatattatt agcactcttt
     2701 gaactggagt gctaattcat aattctattg tatcacttgg tcagaaatag tcaagaaaaa
     2761 agtctgactt tctcaagata aaaagcctga gaccaactca gactttttaa ttcttaaaat
     2821 ggcaattctt cctcttccaa gaccaaatct gccaaatctt ggcctgcatt attttcacgc
     2881 atagcacgtt gggcacgact ttccaagagt tggaatcctg tcacaaggac ttcggttaca
     2941 taattcatct gaccattttt ctcaaagcga cgggtacgca attctccatc aacggaaatg
     3001 agactacctt tggttgcgta gcttgccaaa gtttctgcta gtctgcccca tagaaccata
     3061 ttaacaaaat cagcttcacg ttcaccgttt tggtctttgt aacgacggtt gacagcgata
     3121 gttgctcgcg ctaccgactt gtcattgttg gttttgtgca attctggtgt agacgttaaa
     3181 cgcccaatca tgataacttt attatacata ttttcttcct cctacttatc tattcgtagg
     3241 aaatcaaaaa aagttacaga aatttgtaac ttttcgagga aatttcttat tttttatgaa
     3301 tcatgaaacc tgtcgcctgt tgattggcca taatggtcat atctgtaatc tgaacacggc
     3361 gaggctgact ggtcacatag actactgtgt ctgcaatatc ctgagcttgc aaggcttcta
     3421 ttccttggta aacggacgca gctcgttctt tatcaccatg aaaacgcact gtagaaaaat
     3481 ctgtttcgac aattccaggc tgaatggtcg tcaccttgat atccgttgcg atggtatcaa
     3541 ttcgcagtcc atctgaaaag gtcttaactg ccgccttggt ggctgagtaa acagctgcac
     3601 cagcataggc ataaattcct gcggttgacc ccatattgat aatatgacct tgattggctt
     3661 ttaccattgc tggcaagaaa cagcgagtga ctgccatcaa acctttgaca ttggtatcca
     3721 acatggtcag catatccaac tcttcatagt cttgataggg agctaagcca agagccagtc
     3781 ctgcgttatt gaccaggatg tcaatctgac ctatcgtttc taaaatatca gagcagacag
     3841 tctttactat tgtcatatcc gtgacatcta ggagaaaagt ccaaactgtt tgatttggaa
     3901 aagtttctgc aaactccgcc ttaagagctt ctagtctgtc tatccgtcgt cctgttagaa
     3961 cgacatcctc accctgctcc agataagcac gcgcaatcgc ttcaccgatt cctgaggttg
     4021 ctcccgtaat cactacattt tttgccatct tatttccttc tatcagatgc tgacaagttc
     4081 ctaggcagtc cagtgtttcg ctgggtcgaa cggtgttccg acaacttggt cttctgataa
     4141 ttcaagcacc ccacgttttt gcggagcatt tggcaaatgc aattcacgag gactacacat
     4201 catcccaaaa ctcttttcac cacgaagttc gcctgggaaa atgaaattcc cttttggcat
     4261 catggcacca ggaagagcca caatggtttt caacccaaca cgcgcattag gtgctcctgc
     4321 aacgatttgc actatcttgt cacttgcgac tgcaacttgg cagatgttga ggtggtcact
     4381 gtctggatga gctaccatct cgacaatttc accaacaaca aacttaggtt ccttatcatt
     4441 aacaatttct tctgtaaaac cttccgcctg taattcttgg ttcaaacgag cgacttgctc
     4501 atctgataaa aagacttgac cgcgctctgc aatttcaaac aagctcgaaa cctcaaaaat
     4561 gttccaagct actgtttccc cattttcttt gaggaaaaca cgggctacct tgcctttgcg
     4621 ttccatattc agtttggcat ctccgctatt tttcacgatg accataagga catcaccgac
     4681 atgttctttg ttatacgtaa aaatcattgt ttcctttttc tcctatttca atcctgctaa
     4741 aaaatcgtta atttgctcct tacttttacg gtcgcgattg acaaaacgac cgatttcctt
     4801 gtccttttct agaacaacaa ggctaggaat tccgtaaaca tcccagagtt tggccaaatc
     4861 catatactga tctcggtcca ttcgaataaa ggtgaactct ggattggtct cctcaatctc
     4921 tggtaaggca ggataaatat aacgacaatc gccacaccag tctgccacaa aaaggaagac
     4981 cttcttgccc gctttttcca ctaaagatgc taattcttct aaacttgctg gctgtatcat
     5041 aagacttcct cctcatagac taggtcttca ttttcataga caaaggtata atgacggcca
     5101 tcctcaaaaa tgacgccacc aaccaagctc tccagactgc tttcgtaaac ttgaacatag
     5161 aaggtcgcaa tttcccccat gtcggaaaaa tggtctcgca caatctctgt caactcttcc
     5221 tgagtcttca tgagcttacg gtcatctgca acttttttcg tggcaagagc aaggcttccg
     5281 atacctagca gagccaagcc tgccatccac atttttttag ctttcatacc attcatttta
     5341 acacaaaaaa ggcttcagga caaatgagga agcagcagaa aagcaagtaa aaagcctctt
     5401 cctttaagga aaaggacttc ttatactaag agcaaactag gaagctagcc gcaggctgct
     5461 caaaacacgg ttttgaggtt gtggataagg cgacgctgac gtggtttgaa gagattttca
     5521 aagagtatta ttcttattgc caggcgcctg agttgccaac gtagtaacca tcaggtgtgt
     5581 agatattgcg aagcatctta cctgatgaag ctagataata ccacttgcca ttgtctttga
     5641 cccaatcatt cgctagcatg gctccagaag aacttacata ataccattct cccttgtcat
     5701 aaacccaagt gcttgctttc atggctcctg agcaattaaa ggttcaaaaa actgtccaat
     5761 atcattcatt tttttaaagc attttacact acatcatcca cgctgtagga catagataca
     5821 tcaaatgtaa gagccaaaat atcgaaaaat atacaacttt taaaggcaac atttcatttt
     5881 ttaaagtcta acaaccatag aactgaaatc agagcaaggc atcaacttcc tactcttagt
     5941 acgtctattt tgatggttga agtgaagatt ttttatttaa cgtagacaat ttccaacttc
     6001 ccaaaagcca catctccacg gataatcaag gttttgctgg ttggggctac aggagcgtca
     6061 gccttagctg caccaaagga ggtttctact ttcaaatcta cacgccagtg ttgtggaaca
     6121 tagacggttg catttccgaa ggccacttca atatttaaag ttgcaaaatc acctagcatc
     6181 tctgcattat catagtagat tttagcatcc ccaaaagcga cttccacttg gtcatctacg
     6241 agatcttgat cttgcttata gaaggtcccg ctaccaaaag cgacttcctt atccgtgacg
     6301 actgtttttt tcccattgta ccaccatttt ttttcattcc agaacttact tgaatgcgtc
     6361 agataaccaa caccaagtac caccaagatg ctagcccaaa taagagaatg attggtaact
     6421 ggtaacaagt cataagcgta atttgcaatg atgagcgcca gtaaacctgt aaaaactgcc
     6481 gaagtgagat gacgtctaag gatggactca atggacttat aagcaaaaaa aacaatacct
     6541 agtaaaggcc atattttacc atccaaagaa ggaattccaa aattcccttg cagcaagatc
     6601 caagctgcta aaaccaataa aacaatacca aatgctttct ttttcatgtt ttttacctca
     6661 tgtttcttag attttctttt aggagggatt ggtaatgccg tgagacatga acctccttgt
     6721 gcgtctgata aaaggaaatg gtgcccgttc ctgaaaagga cttgtccact gagtaaatct
     6781 gacggatgtt tgcgatcgtt gacttggaaa ctcgattaaa atagcgaggc aagatagact
     6841 ccaactcata gagcttgagg cgaacctcat aagcttcctt ctggttatga gcgtagatct
     6901 tgctcccttc tgtttcaaag aagagaattt ctgacaggtc tagataatat tcacctgtcc
     6961 ccttgtaaaa agtcaacctc ggagcctttg actcttgcaa gagccgttgc aagtctgcaa
     7021 tttcatctgt caaagtgggg gtcttgatga caatttcagt ctcctctaaa ttgccgtcaa
     7081 tctcaattcg taacttcatc acatctcctt tctgactcta ctatatcaaa gctaagttag
     7141 gaatacaagg gcaaaaaagc aagtggtcaa tttgaagcag taagtggttg taggactagc
     7201 ttaacttaca tgtcagaata ggaagaaaag gaatccccac accaaaccac aagcatagcc
     7261 aaccaagaca tccttgggat aatgaacacc ccctaaaacc ctcaccaacc ccagcaaagc
     7321 tgaaaagatc aagcatacta agccaatagg tagactagca tgcagacagg ccatggagat
     7381 gatagttgcc gaaaagacat ggcgactggg catcgaccgt cccgaactat ccttatcaag
     7441 aaggggacag atatcccaag tttcataagg tctcgggtga ttgattttct tacgaaacag
     7501 ggacaaggcc acaaaacctg atgcaggaat aagcacataa accccaactt gctgaccaag
     7561 tcctagttgc aggtaagtgg tgactagcaa gatcagatag accaggggca tggcgactgt
     7621 catcaaacga ttgaaactgc gtaataggaa taaaagaatt ggacgactag ttagtctgga
     7681 gctgatattt cgataccatt cttgataatt tttcatttat gcttctcata cttttctaga
     7741 acttgttgag atgttttccc cttggcaagc caatccacta gcttgtccaa gcgacgtatt
     7801 tcctgcatca gaggatcatc aatttcctca atacggacac cgcaaatctt ccctgtaatt
     7861 gcaattcgtt cagggacata atatggtgct tgtctaaaaa aatctccgta agtaacatcc
     7921 ctatccagac aagcaagaac atcactcact tcataccctg tcaaccaact ggtcacttgg
     7981 tgaactgact ctgcctttcc accctttcgc tcaacttttg caatcaaggc taagtagaca
     8041 gcagcaaatt tcatattgta taatttctga ctcatacttc tctctttcta tgtgtactag
     8101 tataaataag aaaaagaagg ccgtcaagcc ttcttttgat ttattcttct gcttcatctt
     8161 ctgtaaattg actattgtac aagtcagcgt agaagccacc ttgcgccatc agttcctcat
     8221 agttgccttg ctcgatgata tttccatctt tcatgaccaa gatcaagtct gcatttcgga
     8281 tggttgacaa gcggtgggca atgacaaagg atgtgcgtca ttccatcaaa cggtccatgg
     8341 ctttttggat caattcctct gtccgtgtgt caacagaaga agtcgcctca tccaaaatca
     8401 aaagcggtgc atccttaaga agggcacgag caatagtcaa tagttgtttt tgtcctacag
     8461 acaaggtcac ggtgtcatcc aagatggtat catagccatc tggcaaggtc ataataaagt
     8521 ggtgaattcc cacagcctta ctagcttcca tcattcgttc atcactaatc cctatttgat
     8581 tatagatgag attgtctcga atagttcctt caaagagcca ggtatcctgc aagaccattg
     8641 aaaaggcatc atgcacttct gaacgcgtca tagccttggt atccacacca tcaatgcgaa
     8701 tacttccctt atcaatctca tagaatttca tcaaaagatt gacaatggtt gtcttaccag
     8761 ccccagtcgg cccaacaatg gcaacctttt gaccagcatg agctgtcgca gagaagtcat
     8821 agtcttgaac attgacaccg tccaccagaa tttctcctgc tgacacgtcg tagaaacgtg
     8881 gaatcagatt gaccagagtt gatttaccag aacctgttga cccaataaag gccactgttt
     8941 gaccagtttc tgctttaaag ctaacatgtt caataactgc ctccgaattt gccgcatagc
     9001 ggaaggtcac atccttaaac tcgacctgac ctttgaagtt ttcatcagtc agctgcactt
     9061 gaacagggtt ttggatagaa gaatgcaaat ctaaaacttg attaatccgc ttagcagaga
     9121 ccatagttcg gggaagaacg atgaagagtg ctcccatgag aaggaagccc atgacaacct
     9181 acatggcata agacatgaaa acaatcatgt cactaaagag aggcagacgc gctatcggag
     9241 cagcgtcgtt aatcacatag gccccaatcc agtaaatcgc tacactcaaa ccacttgaaa
     9301 tccccatcat gataggattc aaaatagcca taagacggtt gacaaacaaa ttcaaacggg
     9361 tcaattcatc atttactgct gcaaattttt cattttgata atcctctgca ttgtaggcac
     9421 gaacgacacg aatacctgtt aaactctcac gagtgatact gttcagttta tctatcagcc
     9481 cctgaatcaa ggactgtttt ggaaagtcta gcgtcatcaa aacggtcgtc atcaggacgt
     9541 tgacaatcac tgccacaagt acggcccaaa gccagtattc tgaatgacct aaaatcttcc
     9601 caatagccca aatagccata attggaccac gcgttaccac ttgcaagccc atagtaatca
     9661 acatttgaac ttgagtaatg tcattggtag tacgcgtcaa gaggctagga attgaaaatt
     9721 tcttaatctc tgtctgcgag taatccaaaa ctcggttaaa aatatcactt ctcagcctac
     9781 tagtataaga agccgccact cgggatgcaa aaaatccaac tgcaactacg gacaagaagg
     9841 caagaaagag cattcccatc atcatgcttg ccggctgcca caactcatct aaattagttc
     9901 cttgactacc tagcaaatcc gtaattttcg agatataggt cggcacttcc aactctagat
     9961 agaccgaaaa gcaagtaaag agaatggcta gtaaaatcat ccccattctt ttctactaat
    10021 tcttttggct aatttcttta ttctctcctc ctattccctt gatattttgc ctgtagttga
    10081 ccgagaactt tctcaaaaat cagtaattca tcttcatcaa tgtcttccat caactgcttg
    10141 tctatgcgtt caaaaaaagc cttaacctgt tgcatctgag aacgtgcttt gtccgtcaga
    10201 cgaacaaact tagcccgctt atcaacagga ctcgcctcca attccaccaa accattttgc
    10261 actatacgct taaccagatt actagcaaca gacttggtaa tattgagttc ctgctcgata
    10321 tctttaatca agaccaagtc ttggtttttc tcgcgattat ccaaaaaacg cacaacctga
    10381 ccttgcggcc cacccataaa ttcaatgccg caacgtttgg cttccttttg caccatcagg
    10441 taaatttgat gaccaaaacg cttaaagact aacatcggtt tatccataat ctcccccttc
    10501 taaataaaaa tagttctctg gagaataatt aaatttctat gagaactatt ttcttgatta
    10561 aaaaaatccc aagtgatttt ctcacttagg atcatgttct ataggttaaa ttaaaaccca
    10621 tctacgttcg tataaatctt ttggacgtct tcgtcgtctt caagaacgct gtaaagtttt
    10681 tcaaaggttt caaggtcttc gcctgacaat tccacttctg actgaggaat catttccaat
    10741 tcagtcactt ggaattcttc aataccagac tcacggagag caacgatagc cttgtgaagg
    10801 tcagttggcg ctgtgtaaac tgtgattgta ccttcttgtg cttctacgtc atccacatcc
    10861 acatccgctt cgagcaattg ctcaaagact gcgtccgcat cttcacctcc aaatacaata
    10921 acacctttgt tgtcaaaaag gtaagaaaca gaacctgaag cgcccatgtt tccgccgttt
    10981 ttaccaaagg ctgcacggac attggctgct gtacggttaa cgttagaagt caaagtatcc
    11041 acaattagca tagagccatt tggcccaaaa ccttcgtaac gtccttctgt aaaggtttcg
    11101 tctgtgtttc ctttggcttt atcaatcgct ttatcgataa tgtgttttgg cacttgggct
    11161 tgtttagcac ggtcgataac gaatttcaaa gctgagtttg attctggatc tggatcacct
    11221 tttttagctg ctacatagat ttctacacca aattttgcat atactttaga gttagctcca
    11281 tctttagccg ttttcttggc tacgatattg gcccatttac gtcccattag gaatctcctt
    11341 ttttcacatt ttaatctttc ttattataac acaagttttt ttgattttca ctagaggaaa
    11401 tggattttat tagcaaatca agctaggata gtactttacc tgctaagatg gtcttgcctt
    11461 tctatcttta tcaacagaca ctcatccaca ttcaaaaaac aaactagacc attatctgca
    11521 aatagaaagt ttcagccaag tttgacaaag tcagctcaaa ttactgtttg aagtttgtag
    11581 atataagcga caaaaacaat catactgcac cttttgttga cagtctactc cagacatatc
    11641 atagttccag taaatacttt gaaatccaac agttcttata ggcgctattg tattctaaga
    11701 aatcaataga agagtttcta agcaaacctc taatactcaa taaaaatcaa agagcaaact
    11761 agaaagctag cctcaggttg ctcaaaacac tgttttgagg ttgcggatag ggctgacatg
    11821 gtttgaagag attttcaaag agtataattt acgtgttccc aagatggaga agttagacta
    11881 gtacactggc acttctaaac cattgctagc aattgatttg ttcatattta atttcatttt
    11941 ttccataaat gggtattaga tataaacagc aaaatatttc cgatacgtgt cgttcttgaa
    12001 tttccaatca tctaaaacaa gtaaaggata atcaatcccc tgtatatcaa ggaattggct
    12061 acccttttta cttttttaca cattctgttt gatagattca ttttaacatc acgagcatac
    12121 tccaatggaa atcgctaggc aagagataaa ctttcagata tccgcagaga gatcatcgcc
    12181 tctttttgtc gcaagcattc tcctctccta gtcattttct accttatctt ctacctgagg
    12241 atagagagtt gttccccaaa tagaaatcgt ccgcttacgc actagtggca aatcggtttt
    12301 ttcataaacc gtacgccacc attcccaggc aagcccggta cactctctaa ttttgacaga
    12361 gagattacga acattccctt ttaaaggaat actagtggta aagtgagccg ttaaatcctg
    12421 cccatttctg tcccaagcct taggagtcaa gacttcctta ccttgatgat cataggataa
    12481 ttcattccaa gtaatataat attgggcaac ataggcacca ctatgatcca gcagtaaatc
    12541 tccgtttctg taagctgtaa ccttagtctc aacatagtct gtactatttt gaaaggtcgc
    12601 aactacattg tcacgtaaaa aagaagttgt ataggaaatc ggcaagcctg gatgatctgc
    12661 tgtaaagcga ctgccttctt gaatcaagtc ctctaccata tccaccttgc ctgttacaac
    12721 tcgggcaccc gaacttgggt cgccccctaa aataaccgcc ttcacttctg tattgtccaa
    12781 aatctgcttc cactctgtct gaggagctac cttgactcct tttatcaaag cttcaaaagc
    12841 agcctctact tcatcactct tactcgtggt ttccaacttg agatagactt ggcgcccata
    12901 agcaacactc gaaatataga ccaaaggacg ctctgcagaa attcctctct gttttaaatc
    12961 ctctaccgtt acagtatctt gaaacacatc tcctggattt ttaacagcgt ctacgctgac
    13021 tgtataataa atctgcttaa aattaacaat ctgaatctgc ttttcgcctg aatggacaga
    13081 gttaaaatca atatcaagag aattccctgt cttttcaaag tcagaaccaa acttgacctt
    13141 gagttgttcc atgctgtgag ccgtgatttt ttcatactgc attctagctg ggacattatt
    13201 gacctgacca taatcttgat gccacttagc caacaaatcg tttaccgctc cgcgaacact
    13261 tgaattgctg gggtcttcca cttggagaaa gctatcgcta cttgccaaac caggcaaatc
    13321 aatactataa gtcatcggag cacgatcgac cgcaagaaga gtgggattat tctctaacaa
    13381 ggtctcatcc actacgagaa gtgctccagg atagaggcga ctgtcgttgg tagctgttac
    13441 agaaatatca cttgtatttg tcgacaagct ccgcttcttt ctttcgataa caacaaactc
    13501 atcgggtagc tgattaccct ctttgatgaa acgattttca atactttctc cctgatgggt
    13561 caagagtttc tttttatcgt aattcatagc tagtataaag tcatttactg ctttatttgc
    13621 catcttctac ctcctaataa gttcctggat tgagttgcat aaactcagac ttgttcagcg
    13681 aaatcagccg tggttggact aagtgatcca aaatttcctc gtacaattct tctgagacat
    13741 tgcgtcgccg tctggctaga taagaagtcg gaataaccgt attatccaac ataaatacct
    13801 tatctaagtc aatcaaggtt ggtcttgtaa aaggattacg agctagatcc ggctcttcta
    13861 tcataaagtt cttgaccaaa cgtctggtca agagagctgg tttgaaggtc tgatttttaa
    13921 ccaactcttt gtttttagtc atgctgttgt caatacagat atacatatga ttcttcacag
    13981 ccaaatcgct actaatagtc ggaaaaggca aataaagagc tacaacatct cctctcttaa
    14041 tcaagcaaga gcaccccctt ttctcctaat gtaacataga caggattgac caagtcttct
    14101 gattgactca gaatttccaa agtttgagtt tggcgcgctg tcaatttagt agcatcttgt
    14161 ctcttcaata caaaatgctt gtcgccaata accttgacaa tataatcctt ctccaaagct
    14221 gactggtaaa tccacatcag atgttgtctg tcctgagaac tcaagagaga aggattttca
    14281 agcctcccga tagtctgata aaaatcaaaa acaggagcta actcctgcca atctgattgg
    14341 ctagttgtca aggctagaaa aagggctttg cgagctgata cttcttggtt agccttgaga
    14401 gttactttcc cctccaagtt ttttagaaat cgggaaactc cagaaagcaa atttttctct
    14461 aactgcgaga aataaaaacc tttcgttccc agacataagt ctttcatgtc gctttctcta
    14521 gcaaataaga gctcaaacat ttgatagtaa aagaaaaata tctggcactg ggtcgcgctc
    14581 atcttttcct tatcggcttc tttttttaac cagagcaagg gcgacaggta gctggattga
    14641 gacatttcct ctacctccta ctctttttta actggagcat ctgcactagc tgccacttct
    14701 tttgactgga tactttccca ctggttaatc tcctctgaga taagaccttc gcatgtcttg
    14761 acaaataggg caaaagcctt ggtctttcct gcatatttct ccgtttggca ttgatagagg
    14821 aatttttctt tctccaggag ttgcgcagtt ttttggtaag aaatccaatt ttcctttgca
    14881 ttatacaaat tgataatccc ctcacacagc aagccgagac tggataaggc aaccgaaatc
    14941 aaacggtagc gatcacctgg cataggaata gcacaaaaga cagctatgag gaaacctgcc
    15001 acgatttctg ttatttttaa taccttatag cgcctacgat gttgaacgct tttctttaaa
    15061 aaatgagcta tctgtacgtc taatcgctct gtcaggtaca tttcttctgg cgtcatattc
    15121 gtaactcctt tcatttactt tgataaccat agtataacat atttgaatat caaagtatag
    15181 taaaaactgg actataactt attagacttc ctgcgaaaca aaatatggta tagtagttct
    15241 atgaattatg aagcaagtaa acaactaact gatacacgat ttaagcgtct tgttggtgtt
    15301 cagcgtacca cttttgaaga gatgttagct gtattaaaaa cagcttatca aaaaagtcgg
    15361 acaagctggt aaaatcttgg ctgacagtaa ttatcaaggg ctcatgaaga tatatcctca
    15421 agcacaaact ccacgtaaat ccagcaaact caagccgcta atagctgaag ataaagccta
    15481 taaccgtgcg ctatctaagg agagaagcaa gattgagaac atctttgcca aagtaaaaac
    15541 gtttaaaata ttttcaacaa cctatcgaaa tcatcgtaaa cgcttcggat tacgaatgaa
    15601 tttgagtgct ggtattatca atcatgaact aggattctag ttttgcagga agtctattat
    15661 atatatgcac agacatatga aatctttttg atacagcctt ctttttacat agagagacag
    15721 cagttatgat atatttatcc atacacaaca tcttctgtac caatactaca atattggtac
    15781 attttgatta cacaatcgtt tcaaaaaata tgatggaagc ctgagtattt tatagtaatt
    15841 cccttcctat caactagatc aaccagttga gtgttgaatg ttgaaacacc ctagcctcag
    15901 caaaggacta tgatcagcat ttcctagcca ataggacgct ccaaatcacc tattctaagt
    15961 ccataactag cttggtcagt aagatacaga ctgcaaggcg agactgacgt ggttgatgag
    16021 atttttgaag agtattacaa tcatagattc accagtagac ttgaacgcag atttaactat
    16081 agtagattga aactagaata gtacacctct gcttctaaaa cattgttaga aatcgatttg
    16141 actgtcctga tcaatttgtc ctgttctcgt ttcattttac tatactaaaa aaccacctga
    16201 aacaaatcaa gtgactagag gataaaaaca aagactgggc aaaaagtttt gaaaagcaaa
    16261 aaacgcataa tatcaggtct tgaaaaacct tgatactata cgtttgattg tgggaagatt
    16321 gacttcattt tctcctgaaa ttgaagtttt ttctagtttc cagatataga cgaattcaat
    16381 atagccatct cgctacaatt attttaacca tcgagaaaat ttttcagaat ttcaaaaatt
    16441 tcattttgat ccaatgaaat gtcatcattt tgaagaatag ttgacttcac aggctctaat
    16501 tctatcacaa agcggtgcat gtctgctttt accgcactac ttgatgttat agccgataac
    16561 ttttcatctc ctagcaagga agctgtcaaa cgggtaaggt cattcaaatg ctttttaatg
    16621 gacttactta aatcttgagc acctgtagca gagcgctctc tcatatccag ccaagcttta
    16681 gccttaaaaa taattaatac tcaatgaaaa tcaaagagca aactaggaag ctagctgcag
    16741 gctgctcaaa acactgtttt gaggttgtag atggaagctg acgtggtttg aagagatttt
    16801 cgaagagtat agaccacaat tactcaatac cgaatacccc tgaatggttt ctttttcatg
    16861 caccaatata ttataataat cttcatccaa taataaggct gataaactag catcttggtc
    16921 aaaatataag ggaatttctc gaccgtcctt ctttaatgga tattctggta aaatactaaa
    16981 tagttcaatc atagaatgaa actcaggatt agttgttgta aatcgaaaaa gctgcgcttt
    17041 ctcatctttc tgacttcctt gatactctcc caattctaaa aagtgattca aggtagtata
    17101 aaattcctta ttttttactt catcaatgat gaccatatca taatcttttg ttgtgcgact
    17161 tttaaatcct tgcgaatcca atacgataga ggtagcagtt cccccaatca gaacataata
    17221 gttctgaaaa tccgcaaaca tttcttgaaa aataactttc gtattagctg gcatcatctt
    17281 ctcccagata ctgtaatatc atattttcta gtgcttcact ctcttcctct atacgtgggt
    17341 catcatcatc ttttaaggtc aaataaagag aaatcggatc tacaaattgt ttatcatgat
    17401 tatttttaaa atcattccaa aactcagata caaaaggacg atatttccat atctctagca
    17461 tctttcattt taaaacatgc tgagaaagtg gcaaggataa ctgattgaat tttctctgcc
    17521 atatgacata gctagtattt tcatccgttt cagctaaaaa agttgaatgc gacaaagcat
    17581 aagcaccacc atatagaagg ttagaaacag attttatctg ctttatatcg ccatctggca
    17641 ataaaatccg ttttttgatg ggattaaata aacatgacac ggattttaag aataattctt
    17701 tctttgacac cgtatatgtg taaagcttat tttgcttgtt taaccaatat aaagctttaa
    17761 aagtcctcaa acacctataa attgttgagt ttggaagtcc agtgacttgt gaaagcaaat
    17821 caacatttac tactttttga ccttttgtca ataaaaaggc aatccacgtt aattgttcgc
    17881 taggtgttaa ttccttaggg atttcagtat cattcgcatt gagtactagt cccaatggag
    17941 ggaagaagag gtttccctta aagtctacaa acggaactct agcttgaagt aattgctttt
    18001 tttcactgtc tgataacttc gaaaacacca aaacaacatc catattggct ttttcaccca
    18061 tagtgcgagc ctgagtaaca aatgaactca aactccccct tctcttttct ttaatcaata
    18121 aaaatgattc cttatgaaaa gtcatttgag tatactcaaa cctttttaca aaactgattg
    18181 gtaaagtcaa agtcagttcc tcaatactga catcaacata aaaggattga aaaattgaca
    18241 ttatcttttt gacattcatg ttttttcctt attctatcat ttttattata ttttatcatt
    18301 cggtataata aaatataagt tttttgataa aataaccaaa aactactcgc tatcccctct
    18361 cacattaccc tacatatcgt ttgacatgcg actgatagtt caggaaaact tcaaggagaa
    18421 cttttctctc atccactatg caggacttac tatttcactt ctactcctat aggctcaatt
    18481 tgacactttt ctttttcgaa ttgctcatat gcctcctgaa tagtgagttc gatttatcca
    18541 aatttatttt tgtctatttt gatgaatact tccacgaaga tagtctgaaa atgaatttgc
    18601 accaattttc cttctctttt taagcatatt attccagata tgattgccct cttattcagt
    18661 aataaattac gctttaaacg cttcaatcag tatatctcct gttgtcatat gttctaaaga
    18721 aaattcttct acatatggtt taacatctct taggttatta cttcctaata tcccattatg
    18781 ctttttcgct aagaaaatag aagtcgcttc tcccttacca ataatcttgt tactatcatg
    18841 atttcttgtt aaatctctat ataatgcgta ttcttcagtt ccaatgtcta tgctcacaat
    18901 ctcagctgaa cccttagtta ccaactggtc tatcctagat tttaaatggg gaattgtagg
    18961 tatattgatt tcatcataca cctcttgtgg aataacaatt ttacccaaat agagcttttc
    19021 taaaagatgt tcagtgccaa cccataaaaa tactgaaata caatctgcat caataaatac
    19081 acgactagtc aaacgacaac tcccccctct tcatctagcc catatacaat atcatatctg
    19141 aaagcatcta gtaacagttc ctcatacttc ccttgcgaaa ttctgttatt ttctaaaagt
    19201 tgttcagttg aattaatata ttatcctaat gccatttctt ttttactttc tgacaaagga
    19261 cgatataaac ttgtatcata gcctaatctt gaagctgtct ctataacact aatatccata
    19321 tttttaattt cttctgcatc aaggtatcca tcattcctca atctatataa catagcttta
    19381 tgactgatac cataaaactg acccaatttt ataatatctt ctacttcaag atgagttcta
    19441 ttggcatttt ctctgatttc ctcaaccatc ctatacagtg aagatgggaa aattaaaaaa
    19501 taagaagcaa actgatccgc ttttctttca gtttcatctc cttcaccaat caagataaga
    19561 ctgactgaac tcttcttcac ctcatcataa taaagatgat acagttcatg tgctaaagaa
    19621 aatctttacc ttcctaatgg catgtctgaa ttgactgcaa tgagactgaa ctgagttcct
    19681 ttataacaga ctccgctaat attctttccg agtccataaa ataccagcgt caaattttct
    19741 atcttttgta ccaatttaaa aatatctatc ggcgattcac catcagctcc caactttttt
    19801 ctaagatttg aagctttatt acttaaatcc attcgtgata ttcctttcac ctttttccat
    19861 tttggcaatc atgctttgat tcaaagacaa atactcagca atcttatctt gagtaagatt
    19921 tgaagatatt ctaagttctt ttattctttt tccaacatca catacattta tcacatacat
    19981 ttatcacata catttatcat attcatacac ctctcataaa aagaatagca cactcttgtc
    20041 ataatttttt aaataaaaaa attatgacaa aacaaggaag caatttattg atgctgctta
    20101 aaaatctaaa attgatgagt tcctatcaaa aatcgtatct tcaacctcaa aacagtactt
    20161 aaagctatcc gactcggttt acattgtcaa atttagattt tatttgagca taactttcta
    20221 gtttgctttc tgatttttgt ttaatatagt agcaaacatt gaaggaaata tctccacaaa
    20281 aaaacgcata ctattaagct ttttcaagac ctaataatat gcgctgttct gatttgaaag
    20341 acattccatt
//
LOCUS       DBEROU010000043        19757 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.043, whole genome shotgun sequence.
ACCESSION   DBEROU010000043 DBEROU010000000
VERSION     DBEROU010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.043"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..87
                     /locus_tag="ACL8CL_001683"
     CDS             <1..87
                     /locus_tag="ACL8CL_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGS1330895.1"
                     /translation="STTYRNHRKRFGLRMNLSAGIINHELGF"
     gene            complement(76..723)
                     /locus_tag="ACL8CL_001684"
     CDS             complement(76..723)
                     /locus_tag="ACL8CL_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HGS1330896.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KRTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLENYIRLPAKLES"
     gene            complement(832..1351)
                     /locus_tag="ACL8CL_001685"
                     /pseudo
     CDS             complement(832..1351)
                     /locus_tag="ACL8CL_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
     gene            complement(1388..1516)
                     /locus_tag="ACL8CL_001686"
                     /pseudo
     CDS             complement(1388..1516)
                     /locus_tag="ACL8CL_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CD1845 family protein"
     gene            complement(1578..2447)
                     /gene="rsmI"
                     /locus_tag="ACL8CL_001687"
     CDS             complement(1578..2447)
                     /gene="rsmI"
                     /locus_tag="ACL8CL_001687"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HGS1330897.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGL
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVVLVRELTKIYEEYQRGTISELLESI
                     AETPLKGECLLIVEGASQGVEEKDEEDLFVEIQTRIQQGVKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            complement(2450..2767)
                     /gene="yabA"
                     /locus_tag="ACL8CL_001688"
     CDS             complement(2450..2767)
                     /gene="yabA"
                     /locus_tag="ACL8CL_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HGS1330898.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            complement(2807..3697)
                     /locus_tag="ACL8CL_001689"
     CDS             complement(2807..3697)
                     /locus_tag="ACL8CL_001689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HGS1330899.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQEEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            complement(3694..4332)
                     /gene="tmk"
                     /locus_tag="ACL8CL_001690"
     CDS             complement(3694..4332)
                     /gene="tmk"
                     /locus_tag="ACL8CL_001690"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HGS1330900.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            complement(4552..5349)
                     /gene="proC"
                     /locus_tag="ACL8CL_001691"
     CDS             complement(4552..5349)
                     /gene="proC"
                     /locus_tag="ACL8CL_001691"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HGS1330901.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFYQLLAKAGLLVELGES
                     LIDAATGLAGCGPAFVYLFIEALADAGVQTGLPREIALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGTVMEAVHQAYKRTQELGK"
     gene            complement(5353..6615)
                     /locus_tag="ACL8CL_001692"
     CDS             complement(5353..6615)
                     /locus_tag="ACL8CL_001692"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HGS1330902.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            complement(6625..7734)
                     /gene="proB"
                     /locus_tag="ACL8CL_001693"
     CDS             complement(6625..7734)
                     /gene="proB"
                     /locus_tag="ACL8CL_001693"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HGS1330903.1"
                     /translation="MKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAMLHEAGHEL
                     ILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQIVSAQILL
                     TQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSAQVAAMVQ
                     ADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTGGMLTKIK
                     AATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLAFYAQSQG
                     SIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKGRVQFGAS
                     ALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            complement(7835..9718)
                     /gene="cbpE"
                     /locus_tag="ACL8CL_001694"
                     /gene_synonym="pce"
     CDS             complement(7835..9718)
                     /gene="cbpE"
                     /locus_tag="ACL8CL_001694"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HGS1330904.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELGVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIQLYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHHDTNKSNTKDFIKNLSPSLIVQ
                     TSDSLPWKNGVDSEYVNWLKERGIERINAASKDYDATVFDIRKDGFVNISTSYKPIPS
                     FQAGWHKSAYGNWWYQAPDSTGEYAVGWNEIEGEWYYFNQTGILLQNQWKKWNNHWFY
                     LTDSGASAKNWKKIAGIWYYFNKENQMEIGWVQDKEQWYYLDVDGSMKTGWLQYMGQW
                     YYFAPSGEMKMGWVKDKETWYYMDSTGVMKTGEIEVAGQHYYLEDSGAMKQGWHKKAN
                     DWYFYKTDGSRAVGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVSIEKS
                     ATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNFSTSQDLTSSTSQSSETSVNKSE
                     SEQ"
     gene            complement(9721..10608)
                     /locus_tag="ACL8CL_001695"
     CDS             complement(9721..10608)
                     /locus_tag="ACL8CL_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS1330905.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKDTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHPVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            complement(10598..11059)
                     /gene="lspA"
                     /locus_tag="ACL8CL_001696"
     CDS             complement(10598..11059)
                     /gene="lspA"
                     /locus_tag="ACL8CL_001696"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HGS1330906.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            complement(11056..11964)
                     /locus_tag="ACL8CL_001697"
     CDS             complement(11056..11964)
                     /locus_tag="ACL8CL_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HGS1330907.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            11957..12154
                     /locus_tag="ACL8CL_001698"
     CDS             11957..12154
                     /locus_tag="ACL8CL_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HGS1330908.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLNYLHYLPFSRQSY
                     FQNQFKKISGYTPLQYRKEKR"
     gene            complement(12206..12877)
                     /locus_tag="ACL8CL_001699"
     CDS             complement(12206..12877)
                     /locus_tag="ACL8CL_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1330909.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLMESGVLFLAVIYLLVFAGKKFFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISLSS
                     NQISLNETVEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(12931..13152)
                     /locus_tag="ACL8CL_001700"
     CDS             complement(12931..13152)
                     /locus_tag="ACL8CL_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HGS1330910.1"
                     /translation="MILMTKNINLTNEELELIQGGADSYGKNPNGRYDWEIEPVLTLP
                     VHGFCPRGTYDLGYIGGGNHLCKGSAVRF"
     gene            13424..14233
                     /locus_tag="ACL8CL_001701"
     CDS             13424..14233
                     /locus_tag="ACL8CL_001701"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HGS1330911.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWSLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
     gene            complement(14414..15289)
                     /gene="aguB"
                     /locus_tag="ACL8CL_001702"
     CDS             complement(14414..15289)
                     /gene="aguB"
                     /locus_tag="ACL8CL_001702"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HGS1330912.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(15299..16384)
                     /gene="aguA"
                     /locus_tag="ACL8CL_001703"
     CDS             complement(15299..16384)
                     /gene="aguA"
                     /locus_tag="ACL8CL_001703"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HGS1330913.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRAF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALEKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAELVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEERYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            complement(16381..17508)
                     /gene="nspC"
                     /locus_tag="ACL8CL_001704"
     CDS             complement(16381..17508)
                     /gene="nspC"
                     /locus_tag="ACL8CL_001704"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0045312 - nor-spermidine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HGS1330914.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLGITDHIVF
                     NSERQLRKHGSRCREAGVSVGLRLNPQCSTQGDHALYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFESQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            complement(17508..18767)
                     /locus_tag="ACL8CL_001705"
     CDS             complement(17508..18767)
                     /locus_tag="ACL8CL_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HGS1330915.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTRIETAALDADKVEEVIALIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDAINFNGQDIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            complement(18764..19624)
                     /gene="speE"
                     /locus_tag="ACL8CL_001706"
     CDS             complement(18764..19624)
                     /gene="speE"
                     /locus_tag="ACL8CL_001706"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HGS1330916.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
BASE COUNT         5721 a         4580 c         3516 g         5940 t
ORIGIN      
        1 tcaacaacct atcgaaatca tcgtaaacgc ttcggattac gaatgaattt gagtgctggt
       61 attatcaatc atgaactagg attctagttt tgcaggaagt ctaatgtaat tctcaagctg
      121 tgagttcatt atgatttgta aatccgatat atcttcagca gctaaaaaca cattttgaaa
      181 tgttccaagt ccgttttcac caaaactata ttctctctta ctatacttac tcttattatt
      241 ctctatatag ttagagtcaa ggttcttaac ctcttgaagt tcattttctt tacttctgag
      301 gttaaaatct tttacttctg aagttaagtt ttttgacttc tgtaagtcat tttcttttac
      361 ctgaaatata ctcataaagt ctttaacata aatgatgttc ggcttaccaa gtccaagcct
      421 tactctttcg atcagtccta ttcctttttt tacatcaagc tcatctaatg tttttatggc
      481 agttggcttt gagatatttc ttcttttcat aatttcttcg acagtaaaat agataaatac
      541 tctgccttcc ttgtctatcc agttattctt aaatgacatt cctgtccgtt taagaagtat
      601 ggaataaagg ataattgctt ctgctgataa gccccgaaat tcttctctat caactaatat
      661 ctccggtact tttaagaagt taaatctttc cgcttctctg ttataaaaat attcaaagtc
      721 catgcaagat cacctccctt cttaaaaatt tgcaaagaaa agacgatagt ttttgcttct
      781 atcgcctttg gtaaccattt ttatgaaatt ttttaaagtt aaaaatataa tttataatac
      841 tatcttcttc cattttctga ttttagttct gaaagttcca aaaggtgcaa ccgtatttac
      901 atgaataaac ttatacactt cccaagtcgc tgttttagtc gcttcatcag cccactttct
      961 catatgcggt tcaaataatt cttcctcact caacgaatca atcattgcag agatagaatt
     1021 aatattttca tttaattttg ctttcaactc ttgcagagat aaatgagcgt aggtatctgt
     1081 gaaccactga tataaattca ccaagttgat tccatttaaa tttatccgat ggtgttttta
     1141 cttgaagccc ctttctttca tcttcttccc atttaagaac caagttggtc cagccaacct
     1201 gataagcaag gttttctgct ggagttctat caacttcatc aactctctta tcttttaaat
     1261 tttctggaat attatcaaat tctgaaatat atttctcaaa tgttttctct atctcagctt
     1321 ttagttcttc tttattttcg tatgttcgca aaaaatcacc ttctttctga tttatcaatc
     1381 ctttgattta aaatgacctt atttttctat ttataccttc taggaacact ataactgctg
     1441 ctccaaccat cggtattccg tatgggctta ataaaaaact taataccaaa gcttctatac
     1501 caagtgatac atctttatat tatcatcact aattaagtta aacagaaaca gacagaatta
     1561 ttacatcctg tctcccttta ttgtttttct tcccagtcgt ggtaggcagc gtagagctga
     1621 cttttattcc actggtaaat cttagcgact tccttgatag cttggttttt cttcacacct
     1681 tgctggatgc gggtttgaat ttctacgaac aagtcttcct cgtccttttc ctccacaccc
     1741 tgactggcac cctcaacaat gagaagacat tcgcccttga gtggcgtttc agcaatgctt
     1801 tctaataact cagagatagt acctcgttgg tattcttcat agattttggt caattccctg
     1861 accaagacaa ctgagcggtc accgtagact tctaacatat tttccaacgt gtctgctaca
     1921 cgatgaggtg attcataaaa aatctgtgtt tcaggataat cttttttcaa gccaaaaaat
     1981 tgcttctgct gacctgattt tctcggtaaa aaaccgtaaa agatatgtgg ctgtggcgct
     2041 aaaccactgg caatcaaggc agaaattcct gcagaggcac ctggaactgt cacaactgca
     2101 atttcttcct caatagctgc cttaactaaa tcatgaccag ggtctgaaat gctaggcaaa
     2161 ccggcatcag agacctgagc aatactttgc cctgctttca agaaaccaat caaatcagga
     2221 attttttcct tggcattgtg ctcatgaaaa ctgatctgct tagtggaaat gtcaaaatgc
     2281 ttgagcaaaa gacctgtatt gcgcgtatcc tcagcagcaa tccaatctac ttctttcaag
     2341 gtctggatag ctcgaaaggt catatcatct agattgccaa tcggcgttgc cactagatac
     2401 agcttgccat agggagactg ccccttaaaa cttttttgaa tctgcatgcc tactccctgt
     2461 atagcaactc gtcacaaaac atacattcct cgtcctgctc tcgacgttgt ccataaaaat
     2521 cattacatac gtgaaatcca tcacggtaaa tgcgacggac actttcacga acatgcttgg
     2581 ccttgacagg agcatctgct tccacctcac ccaagcgttc tcgcaactta ctattttcca
     2641 agcgaagagc tgtattttcc tctaccaggc tcttgagatt tttcttgatg gcttccacat
     2701 cggctaaggt taccaataat tgttgggaaa aatcatccag cgcgtcaaat aattcttttt
     2761 tgtccataaa acagcccttt cctttcttta tcattcattt ttgagtttat atttctttca
     2821 agaccagata ttccatggca ttttgaaagc tgacattagc ttgccacatt tttctagctt
     2881 ctagtaaatc ttgtagaatc actcttactc ttacctgcaa gaggtcctgc ccacagagaa
     2941 cttcaagaat ccgtaaaacc tgatcctgtt tttccttatc atctgccaag ttggctaatt
     3001 tggcaacctg tagataactt tcttttttct tagctactaa ccaagtcagc aggcgttcac
     3061 tttcatcgac caaggtccaa aaacttgcct gattagccaa cttttctgct tcagctcgcg
     3121 attgactaaa cttagctaaa agagtcgctt ttttcttaac aagtcccatt tgttctaaga
     3181 gtaagataag tttttcttct tgctttttaa agtggaagat ctgagtccga cttcggattg
     3241 tcggtaacat cttttcctca tcgctagtca agaagaaaat ataaacttca ctctggggtt
     3301 cttcgatgac cttgagcaga gaattggctg cgttgggatg cattttatcc gcttgctcga
     3361 tgataaagac ctgttgctgg ctttcaatcc ctgcttgaga aaactgtccc accaattccc
     3421 gaatgcgttc tgtcttgatg acctgattga ctggcttaat caaggtgaca tctggaaact
     3481 cttcctgttc aatcagcttg caacttcggc atttctcaca tggtaagacg ccaactttat
     3541 ccgtacaaaa gaggctctta gctaaaaatt gcgctatttc caagcttcca aagaaacctg
     3601 aaaagagata ggcgtgattg agctggtctt gttctaagat acggacaaaa cggtcaaact
     3661 gagctggttg ccaagccttt agttgatctt gtttcatttg gccaagccca ttccgtcaaa
     3721 caagacagcc ttggtagttt ccacaacttg ctccaaaggg agactagcat caatcttgac
     3781 aatgcgattt ccctctttat ccagaagaga aaggtagcct tgacgaactt ttttatgcaa
     3841 gtccaaccct tccaaatcca aacgattaac ctcgcggtca ctattagcag caatacgagc
     3901 cagcccttct tccacctcga tgtcaaaata gagtgtcaaa tcgggtttga ggccatctgt
     3961 cgcaaactga ttgagccagt caatggcttc aatatctaag ccacgaccaa atccctgata
     4021 ggcaacagaa ctatcgataa aacgatccat gatgaccaac ttgccagctt caagtgctgg
     4081 aagaactttt tccaccaaat gctgtctgcg actggcaata tagagaagta gctctgtttt
     4141 agcatccatc tgagtatgac ttggatccaa aatcacttcc cgaatcttct ccccaatcaa
     4201 gactccgcca ggttcacggg tcgtcaacac ctctactcct ttttcctcta aaattggtag
     4261 cagagcctct aaaacactgg tcttgcctgc tccctctggt ccctcaagag agactaaaaa
     4321 tccttttgac atgtctaact catttctttt ttactacttc tattctatca aaaaaatagg
     4381 ggtttgtgac aatatttttg tcttctcatt tcatactcaa tgaaaatcaa agagcaaact
     4441 aggaagctag ccgcaggctg tacttgagta cggcaaggtg aagctgacgc agtttgaatt
     4501 tgattttcga agagtatcag ccaccataaa agaggcagtc aaagctacct cttatttacc
     4561 tagttcttgt gttcgtttat aggcttgatg aactgcctcc atgactgtgc ctcgaaaagc
     4621 atgcgcttct aggcttgcta cgccagcaat agtcgaaccg cctggactac aaacttgatc
     4681 tttcaaaact ccaggatgtt gctgactttc aaggaccaat tgcccagctc ctaccacagt
     4741 ttgtgctgcc attttcaatg ctatttctcg tggtaatcct gtctgaacac ctgcatctgc
     4801 caaggcctcg ataaaaagat agacaaaggc tggtccacaa cctgcaagac ctgtcgctgc
     4861 atcgattaaa ctttctccta gttcaaccaa gagaccagcc ttggctaaaa gctgataaaa
     4921 gagctcactg tcctcagccc tgcaattagg agacaaggca taactaatca ctccttgccc
     4981 gatagaagca ggggtattag gcatcatacg aataattcgg tgttgacttg ggataagact
     5041 tgctagtttt tctaaggtca atccagctgc catcgaaatc aaaagaagac tttctctttt
     5101 ttcaaggatg gtctggtatt gagaaagcag ttcagaaaac tgagcaggct taactcctag
     5161 aaaaatcaca tctgcttctg caaacatttc ttcattgctg gaagcctgac caccaaagtc
     5221 tgcaatgaaa gcatctacct tagcttgact acgattggca aggagaatct catctgacgt
     5281 cctagtctgc aagacagatt ttgccaagct agcacccata ttccccaaac cgataaatcc
     5341 aatcttcatc tcttactccc ttatctgccc atcaccggca accacatact tgtagctggt
     5401 caactctttc aagcccatgg gaccacgcgc gtgcaatttc tgagtagaaa tccccatttc
     5461 acaaccaaga ccaaattgtc ctccatctgt gaaacgagtt gaggcattaa catacaccgc
     5521 tgcagagtcc acttgatctg taaagtatgc tgcagcttca gcattttccg tcacaatagc
     5581 atccgaatga tgggtgctgt gggattcaat gtgcgcaacc gcttcttcta aactgctcac
     5641 aaccttaaca gcaaggacat agtctaaaaa ctcggtgtca aagtcttggg tctcagctgc
     5701 ttgacctgaa acaaactggc ttgctttgct atccaagcgg aattgaattg gttccagtcc
     5761 ggcttcctta cgctctgcaa ccagcatttg atccaagcga ggaaggatgc ttgctgcctt
     5821 gttttcatga accagcagaa cctccatggc attacaaaca gaaggacgac tggttttagc
     5881 attgttgatg atagacagcg ccttgtcttc gtctgcatcc ttatccacat agacatggac
     5941 aatcccagtc cctgtctcga taacaggtac aatcgcattc tcaaccactg cattgatcaa
     6001 gccagctcct ccacgaggaa tgagaaggtc tagatagccc ttggccttca tcatagcata
     6061 actactttca cggctagtat cctccaccag ttgaatcaca tttggatgaa tagtagtcgt
     6121 ctccaagccc ttcttcaagg ctgtgacaat ggcatgggtt gtttgatagg catccttacc
     6181 actacgaaga acaaccgcat ttccactctt aagagtcaaa gcagccgcat cagacgtcac
     6241 atttggacgg ctttcataga taataccgat gacacccata gctacacgtt tttttgtgat
     6301 aaccaaacca ttttcaagct gacttgtttc taaaacttca ccgattggat ctggtaaggc
     6361 aaccacttca cgaattcctc ttgccatcgc ttctatacga tctgcatcca aataaagacg
     6421 atccaacatc acatctgaga ttttcccctt agccgctgcc atatcgaggg cattagccgc
     6481 taaaatttcc tcagtagcag ccactaagtg atcagccatg gctagcaagg cttggttttt
     6541 cacttcttca ctagctgtgt taatcgattt tttaacagcc tgtacctgtt caaattgttc
     6601 ttgtctactc accatagttt acctctaaaa ttctgtaaaa agtagttgga tttcaggagt
     6661 aatggaaatc cagtcgtcac ggtaaatcaa gacacccttg gctttttgag aacgcaacat
     6721 atcctccaaa gcagatgctc caaattgcac gcgtcctttt ccaagtgatt ttccactttc
     6781 cttgtcaaat actgtcacga tatcaccgta agaaaaggct ccttctgctt caacgatacc
     6841 agataagaga agactctttc catgttgaga gagagcttcc gcagcccctt tatcaaccca
     6901 aatagaacct tgactctgag catagaaggc aagccattgt ttctgggtac gaagcccctt
     6961 ctcttgagca acaaagtaag aaccatcctc ggtctcctct gccgcctcaa tcatggaatc
     7021 tgatttcaag gatgagcaga tataaacagg aactcctgat tccgtcgcga tagttgcagc
     7081 cttgattttg gttaacatac ccccagttcc gtttgacgaa ccagctccac cagccatatc
     7141 aataatctca cgattgatgg tctcgattct ctccaagcgt ttggctcttg gatctgaatt
     7201 aggatttcca gtatagagac cgtccacatc tgtcaagaaa actaaaaggt ctgcttggac
     7261 catcgccgct acttgagcac ttagagtgtc attgtcccca accttgagct catcaataac
     7321 gacactatca ttctcattga tgataggaat tgccccacgg ttgagcaaaa ccgacaaagc
     7381 ctgatgggca tttttataac gacgcttatc cacaaagtca tcttgggtca gcaagatttg
     7441 tgcagaaacg atttgacgca agagaagatt ggtcgtatat tcttccaaca aaagtccctg
     7501 ccctaccgct gctgaagcct gtttatcagc aattttagtc ggacgctttt taaatcctaa
     7561 ggctccaaaa ccagccgcaa tggcacctga agacaccaaa atcaactcat gaccagcctc
     7621 gtgcagcata gccaactgct gggtaatatc ctttacctta ctacgtgata aacttccatc
     7681 ctcatttgtc agagaagaag tacccacctt aaagacaatc cgtttgtatt tcatagtctc
     7741 actccgatta ttcatattta tccattataa catgaataac aggaaataga aaagagttga
     7801 taggaaaaag aaggccctaa aaccttcttt tctactactg ttctgattcc gatttgttta
     7861 cactcgtttc agagctttgt gaagttgagg atgtcaaatc ttgactagtt gaaaaatttg
     7921 taacactttc atgttgactc gtttctttat tttcaagatc ctttttcact acttctttgg
     7981 attcttttat ttctgaatga cttgtagttt taattgtagc agatttctcg atcgaaacat
     8041 ccactaacca ggcaccactt gaatccacag tataaccttc tggtgtctta ccgttcacaa
     8101 gtaattgacc attttctttc aagaagtacc atttatcctt gtccttgatc caacccacag
     8161 ctcgtgaacc gtctgtcttg tagaaatacc aatcatttgc ctttttatgc cagccttgct
     8221 tcatagctcc tgaatcttcc agataataat gttgaccagc aacttctatc tcacctgtct
     8281 tcatgacacc agtagaatcc atatagtacc aggtttcttt atcttttacc cagcccattt
     8341 tcatttcccc tgatggagca aagtaatacc attgccccat atattgaagc catcctgtct
     8401 tcatagaacc atcaacatcc aaataatacc actgctcttt atcttgaacc caaccaattt
     8461 ccatctgatt ttctttgtta aaataatacc agattccagc gattttcttc caatttttag
     8521 cagaagcacc agagtctgtc aaatagaacc aatgattgtt ccattttttc cattgattct
     8581 gtaacaagat acccgtttgg ttaaagtaat accattcacc ttcgatttca ttccaaccga
     8641 cagcatactc tcctgtagaa tcaggcgctt gataccacca gttcccatat gcactcttat
     8701 gccaaccagc ttgaaaactt ggaatcggct tgtaggatgt tgaaatattg acaaaaccgt
     8761 cttttcgaat atcaaaaact gttgcatcat agtctttgct ggctgcgttg attctctcaa
     8821 ttcctcgttc tttgagccaa ttaacatact cactatcaac accatttttc caaggtagac
     8881 tatccgaagt ttgaacaatc aaactcggac tcaaattttt aatgaaatcc ttggtatttg
     8941 atttgttggt atcatggtga tgattaaact tcatcaaatc aacttttcca atgagaggac
     9001 catacttgtc ttctgctcca tgaacattat ctaaatcgcc cccaaggtaa attttcttgc
     9061 cattgacttt caccacgcta atcaaggaat tggaattgtc atcccaaatt ttctttaatt
     9121 cacccgatga atcagtttca ttttcataat tatagagctg aatatccatg tccccaaact
     9181 gaaaatgagc atccccttgt gtgatatttt gaataactga aacacctttt tctgcagcag
     9241 tctgtaaaac cttatcatag ccatacagat tatcccatag acgttcagaa ttagtaatac
     9301 gactatcact atatttctta agatagactc ggtcaactgg ataggtagac agtaattcat
     9361 caacatttcc aatatgatca ctgtgggtat gggtcaccaa aataaaatca agtttttgga
     9421 cacccaattc cttcaaacga cgaaagacac ggtctgttag aacatgctta taagacgttt
     9481 caattccttc tctccatgga taacgagaat cacttccatc tgggaaatca taatcttctc
     9541 ctgtatccac catggcaaaa tgtccattgc tttcaagaat aatcgcatca ctgccacctt
     9601 cttgaacatt gataaagtgg attttatttc ctgaactttc ttgagcctga acattcccat
     9661 accatgacaa acctaaaaaa gcgcctacaa gtgctaaact agttaatttc tttttcattc
     9721 ttactttctc aatctctcca aggtttcctt aaaaatctct gggatatctg ctttaaattc
     9781 caaggtctta cctgttctcg gatgagtaaa acctaaagtc ttggcatgaa gaaattgtcc
     9841 atgtcctttc aaagtcttgc gaggaccata gacctcatca ccagcgactg gatggccgat
     9901 ataagccatg tggacacgga tttgatgagt gcgccctgtc tccagttgca actctactaa
     9961 gctataatcg ccaaagcgtt ccaagacgtg aaaacgcgtc actgcaggct tccctttagc
    10021 agttacagcc tgtttcttac ggtctttttc actccggcca atcggcgctt caattacacc
    10081 acgatcatta ggcagatttc catgaacaat cgcccaatat ttgcggagag actttttatc
    10141 tttgagttct tgggcaagta ctagatgcgc atcatcgttt ttagcaatca tgagaagacc
    10201 tgacgtatcc ttatcaatac ggtgaacaat ccctggacgc agaaccccat tgatacccga
    10261 caagtcctta atatgataca tgagggcatt tactagggtt ccactggtat gaccagcact
    10321 cgggtgcaca accattccct gaggtttgtt aacgacagcc acatcctcat cttggtagac
    10381 tatttctagc ggaagatcct cagccacata ctctaatacc tctggttctg gcacatggta
    10441 agtgacgaca tcaccctctt ggactgtgta tttagctttc ttgacttgac cattgaccaa
    10501 gacctggcct gatttaattt gttcattcgc gagactacgt gataattctg acaaatctga
    10561 caaagcctta tccaaacgca gaccaccagt ttcaatttta atttccattt atttcctctt
    10621 ttagcattgc aatcaataaa ataatcactc caaccgtcag atagctatct gccacattga
    10681 aaattgcaaa gttgataaag tcaaggtgga acatatccac aacaaagccc tgactgaccc
    10741 tgtcaataaa gtttccaaga ccacccgcga ttattagagt caaacccaag accatccaga
    10801 atgagtcctc catgtgttta tgtaaatacc aaatggcacc tatcacgaca accagagtaa
    10861 tgacagcgaa taacagctgc tgatcttgta agatagaaaa ggctgcacct cgattttgca
    10921 ggtaggtcaa gctaacgaaa ttgggaatcc aggagcgcac ttcacccagt ggaatctgct
    10981 ggacgatata ggatttgacc aactgatcca gcccaattaa aagcagtaca atgactgcca
    11041 ctattgctct ttttttcatg atttcctctt ttgatcaaaa tattcttgca tgacttctac
    11101 gaagagagtc ccagcttgac taagctccac ttcttcacgt ttaacataga ccatgcggtt
    11161 atctaggtta tccttgagac gaataactgt aatgccatta acactgtcac tatctaaaaa
    11221 tccagaacct gtcgcatagg cgtccgtccg ctccaaaata ccattcaagg tggcacggtc
    11281 tgtcacatta aacatctgtg agctagcgct ggtatcgaca aagttctctg aataataaag
    11341 gtactcgtct ttctcttgag tgaaacgaac cgttggtaaa tccgctaaat cctccatgac
    11401 taattcctct ttctgggcta aaggatgacc ctcacagaga taaatatggg tatggaaagg
    11461 aatcaattcg atgacctcca gacctaactt ttcaacccgt tgcataatcc cctttttatt
    11521 ttgattgttg aggtagataa tcccaatctc actatgccct tgcgccactt catctaatat
    11581 ttgaacagta gttgattcaa aaatacggaa gttcttatag tcaggatagc gctctgaaaa
    11641 ggccgtaata gttggtggta agaagtcata gtgctggcta gcaacggaaa attcatcttt
    11701 ttcttcttca ggattggcat actgattttg aaaaatatca aatcctttaa ccaattcttg
    11761 cgctttttca taaaattcca tcccacgacg ggtcaagaaa gtccctgagc tggtccgacg
    11821 gaaaatctta aagcccaact ctttttccaa atcacgaaca gaaatagaca gactcggctg
    11881 actaacatac atcttttcag cagcttcacg aaaagtacca ctattggcaa tagccacaac
    11941 atagcgtaat tgttgaatgt tcatcttcta cccccaactt ctttatcttt tcattatacc
    12001 atattttaga agttttccaa aaaggaaaaa agaacatcct attcttctta actatcttca
    12061 ctatctgcct ttctcacgcc aatcttattt tcaaaatcaa ttcaaaaaaa taagtggcta
    12121 cacaccactt cagtatcgga aagaaaaacg ttgactattt gtgaaaaaag aaaattccgg
    12181 aaagttccga cattatttta gtaagctaac ttcctgaaaa taggagcaac cacacatccc
    12241 agaaggcagt gatattaatg agaatatgaa ctagtattgg atagtagaga tttttagtca
    12301 tgcgatacaa tagagctaaa ataagacctc cactagcata aatgaaaaaa gcaagaggag
    12361 ttaaagcaaa attgatatga atataaccga aaataatagc agaaagcaaa acatctccgt
    12421 accaaggcga gtttttgaaa aaggttgtca taagcacacc gcgataaatc aattcctcag
    12481 caataggggc gatgaagcaa acgatgagca agaaataagg gaactcctgt ctccccatca
    12541 tttctaccgt ttcattcaaa gaaatctgat ttgaagacag ggatatgaaa tacgaaaaga
    12601 ggaagtcaga catatatgaa atgatgtagc ccagtaaaag gtagatgaag tacctcagct
    12661 gccacttgaa atgaaaaaat ttttttcctg caaaaaccag tagatagatg accgctaaga
    12721 aaagaactcc gctctccatc aaaagcagaa tctgaaaaaa ttcacgactt gctggtagat
    12781 agggctttgc gaggtgattg aaaactctcc aaaaccaagt tgatttgtaa aaaattaagg
    12841 acaatgctag taaaatttga atagcccgtt ttttcatatt aaattctctg ctttctccac
    12901 ttgtttcttc atattactaa taaattttac ttaaaatctc acagcacttc ctttgcaaag
    12961 atgattgcct cctccaatat atcctaaatc ataggtgcct ctgggacaaa atccatgaac
    13021 cggcagagtt aatactggtt ctatttccca atcgtaccta ccattaggat ttttaccata
    13081 tgaatctgct ccaccttgta tcagctctaa ttcttcattt gttagattta tattttttgt
    13141 cattaaaatc atgatatatt tcctccatta ttcttcagat tagttgagta atctgatact
    13201 catacctact tacaaaaaaa ctattatatt aagttggttt tttaatttgt ctagttgcaa
    13261 cattgacaaa catagtatag catatctttg ccaaatatcc tcttcaaatc atgaattgtc
    13321 atcaaaacat cttaaactat aaaatcaatt agtctcaagc tttctatcaa tttcttctcc
    13381 aaaatatgct ataatactac caaaagataa agaaggaaga cctatgatta aactactagc
    13441 cttggatatg gatggaaccc tcctcaatga agccaaggaa atcccacagg ctcacatcgc
    13501 tgctattcac caagccattg aaaaaggtgt caaactggtt ctctgtacgg gtcgccccct
    13561 tttcggtgtc ctcccctact acaaaaaact ggggcttgac ctccagaacg aatatgttat
    13621 tgttaacaac ggttgttcaa ctcaccagac cagtgattgg agtctagttg actggcaaga
    13681 actgagtcca gctgacatcg aatacctcta tgaccttgct gaaaagagtg atgttcagtt
    13741 gacacttttt gacgagtcac attattttgt tctcggtgga aagcccaatc aagttattga
    13801 aaatgatgct aaactagtat tttcagacct gactgaaatt tctcttgaag aagcgactag
    13861 tggaaagttc cggatgttcc aaggtatgtt tttaggaaca aaagaacaaa cagacgattt
    13921 tgagcagcgt tttgctgaag agctttgcca acgattcagt ggagttcgtt cgcagcctgt
    13981 catttatgaa gcaatgccgc ttggaacgac aaaggctact gctctttcac gactagctga
    14041 gattttgaag attgattcct cagagattat ggccatgggc gatgctaata acgatatcga
    14101 aatgctccag tttgcagggc ttgggattgc aatgggaaat gccagcgatt atgtcaaatc
    14161 tcttgcggat gccgttacct caagcaacga agaagacggc gttgcgcgtg ctattgagaa
    14221 atatattcta taaaaaagaa aagcaaatag acagaagtta ctgctatttg cttttttgct
    14281 aatattttaa aatacggact aatcaataga caagaatagc gaatcataaa aatcgattta
    14341 ctagatgcca tagtatctta tagttgctaa taagaagtta gtctagcaga atgaatctct
    14401 catttctccc acactaatct gtaatttgtc tatacatttc tggtcttcta tctcgaaaca
    14461 agccccagtt taggcgttca cttgctccct tgtctaggtc ataagtagct aacagaacag
    14521 cttcttcttg tctttcagct cgttctagaa tagctcctgt ttcatccgtc ataaaggagg
    14581 aaccgtagaa gtcaagactg gaactctgtc cgccattttc ctcactagga gtaacctcct
    14641 ctaaaccata acgattggct gcgatgactg gaacaatatt cgctgctgcg tgcccttgca
    14701 tagtacgttg ccagtgacca caactatctg tatccaaaat tggctctgaa ccgatagctg
    14761 taggataaaa gagcaattca gcaccattca atgcaagaca gcgcgctgtt tcagggaacc
    14821 attgatccca acagataccg ataccaatct tagcatagcg agtattccag accttgaaac
    14881 cagtgttacc aggcgtgaaa tagaattttt cttgataata atggtcatct ggtatgtggg
    14941 tctttcgata aacgcccagc acttccccat ctgcatcaat gacggcaata gagttataca
    15001 agacattacc atctttttca tagaaactga ttggtaaaac aacttgtagt tccttagcaa
    15061 tcaccttaaa atgctgaatg gcagtatttt ccgctacaga ttgggcatac tggtagtagt
    15121 catactgacg ttcctgacag aaatagggat gttcaaacaa ctcgggcaag agaataattt
    15181 gggctccttg ctcagcagcc tgacgtacta aacgctctgc ggtttggata tttgttgcca
    15241 catccttagc gcattgcatc tgaatggttg caactcttac atttctcatc tttttctcct
    15301 attctggaat ttgttgggtg atacagtgga tattgccacc acctaagaga atatctctgg
    15361 ctggtattcc gacaacttta cggtctggga aacacttgct gaggatatct aaggccactt
    15421 ggtcgtttac atcctcaaac tgtggaacca agacagcctt gttggcgata taaaagttta
    15481 cgtaggaagc tgctagtcgt tcacctgcgt atcgctcttc ttctccttct tcatagatgt
    15541 agcctggcaa atcttcttct gtcacaactt gtcgaactgc agggataggc aatttatgaa
    15601 tggtgaagtg acaacctttt gcatctgttt cctgttctaa gagttcgaga tctgcttttg
    15661 acatggcata ctggggatca ttttcgtcat ctgtccaagc caaaacaagc tcagcaggac
    15721 caacaaaggc agcaacatta tcgacgtgtt cattggtttc atcctgataa ataccataag
    15781 gaagccaaat aactttttca gcaccaagac tttctaataa tgtgttttca atctcctctt
    15841 tagtcaggtt aggattgcga ccaggactaa gcaagcaact ttcagttacg agaatagttc
    15901 cttgaccatc gctatggatt gcgcctcctt ccagtacaaa aggtttagca tcatagacag
    15961 gcttttccaa ggcctcagca aaacgactgg ctacttggtc atcctcttca taatcttgat
    16021 aaagaccatc ataggtgcct ccccaagcat tgaaggccca atccacggct aatttcttac
    16081 ctttatcatt gacgagaatg gttgggccag tatcacgcgc ccaggcatca ttggtgggaa
    16141 tgtctaaata aacaaccttg tctccaagat aggattgggc ttcagataga taggcctgct
    16201 ccaccaaaag atagactctt tccccttctg ctatggtctc gatgatctga gtaaatgctc
    16261 ttttagcagc ctttccttga aaaggccatg atcctggtcg agtcggccat atcatgaggg
    16321 taccatgatg gggttcgtac tctgctggca tgtgatagcc taattttttt ggactgtcca
    16381 tcatgataat ctccctttaa agtcttgata gccaaaagct ttgagtaagc tacagtctcc
    16441 ctgttcgtcc atgagataga gacttggcaa tccaatacca ttaaaggtat tatttttgac
    16501 aaaagaataa atggccatgt cttgaaaata aagtctgtct ccgatttgga ctggattttc
    16561 aaaactataa tcaccaatca catcgccagt cagacaggta ttagaagaaa gtctgtaggt
    16621 atgggctttt tcctgtgact caaagccatt tctcaaaggt ggacgatagg gcatctcaag
    16681 tacatcaggc atatggcagg tcgcagaggc gtctaaaacc aagatttcca taccgttttc
    16741 tacaatatct aatacctcag ttgctaaata acccgcatta agcgcaatgg cttcaccagg
    16801 ctcgatatag atttcaagat tgtaagtttt tcggatacgc ttgatttctg aaatcagcaa
    16861 atccacatcg taaccttctc ttgtaatatg atgaccacca cccatattga gccattttac
    16921 ctcatgtaag tagggaccaa actgttcttc tactgctttc aaagttgttt gtaaatcatc
    16981 tgctccctgc tcgcaaaggg tatgaaaatg aagtccgtca accaaatcta gcaaatcact
    17041 cggaatcttg tctatagtaa ctccaaagcg agaaccaggt gcacaagggt catagagcgc
    17101 gtgatctcct tgagttgaac actgagggtt gaggcgcaaa ccaacactga caccagcctc
    17161 tcgacaacgc gaaccatgct tacgcaactg tctctctgag ttaaagacga tatggtctgt
    17221 tatcccaagc aattcctcca tgtcctcatc cttgaaagct ggtgcaaaaa catggacttc
    17281 accaggaaat tcttcccttg ccaatttggc ctcatagagt ccactagctg tcgtacctga
    17341 tagatactgg ctaatcaagg gataagtttt gtagagggaa tatgccttct gggcaagcaa
    17401 gaccttgcaa ccggcctctt cttgtacata ttgtagaatg cggcaattag cttctaactt
    17461 ggccaagtca ataacatagg ctggtgttgg tacttgttct aacttcatta gtccaccatt
    17521 tgtggatttt caaccacaac ccatggcaaa ccatactcat tcaaagcttc catgaatgga
    17581 tctggatcta actcctcaag gttatacact ccagcttgtt tccaagtacc gttcataact
    17641 aattttgtcc caatcatggc tggaacgcct gttgtgtaag aaatagcttg tgaaccaact
    17701 tctgcgtaac attcctgatg gtcgcaaaca ttgtagatat agatggtctt ttcaacaccg
    17761 tccttgacac ctgtaaagat acaaccgata ttggttttac caacagtgcg cgggccaagg
    17821 cttgcagggt ctggaagcaa ggcttttaag aattgaatcg gaacaatgtc ttgaccgttg
    17881 aagttaatag catctgtacg aaggagtcca acattttcaa gacatttcat gtgcgtcaag
    17941 taagattgac caaaggtcat aaagaaacga atacgtttaa cacctggaat gttcttagcc
    18001 aatgattcga tttcttcgtg gtgaaggaga tacatgtctt tttgtccaac ttgagggaaa
    18061 tcgtactcgc gcttgataga catggcttca acctcgaccc attttccatc ttcccagtaa
    18121 gaacctggcg cagaaacctc gcgtagattg atttctgggt taaagtttgt cgcaaatgga
    18181 taaccgtggt caccaccatt acagtctaaa atgtcgatat agtggatttc atcaaaatag
    18241 tgtttgaggg cataagccga gaaaacacta gtcacacctg ggtcaaaacc agaaccaagc
    18301 agagcggtca agccagcttc tttgaatttc tcctgataag cccattgcca tgagtagtca
    18361 aagtaggctg taaaaccaag ttccttgcaa cgtttttcat aaatagcacg ccactcaggg
    18421 tcttctgtat cctcagcctc gtagttggct gtatcgatat agtgaacacc tgttgccaag
    18481 caagcatcca tgatggtcaa gtcttgataa ggcaaagcta cgttcaacac agcttctggt
    18541 ttgtagcttt caatcagggc aatcacttct tcaaccttat cagcatcaag agctgctgtc
    18601 tcaatccttg tacttgtttt gccttccagt ttagctttca agtcatcaca ttttgactta
    18661 gtacggctag caatcataat ctctgtaaag gtttcgctat cttgacaaat ctttgaaata
    18721 gcaacttggg caacgccccc acatccaata actagtaaac gactcatttt tttccttcct
    18781 cttcttctaa aatgtcctca acatacttgg gcaacataaa ggctcccacg tgtaagtttg
    18841 cagtgtagta ttctgtgaaa agctgacgtt ttttccagcc ttccttgtca aaatctttga
    18901 cagggtggta tttttttgat gcaaatccaa acaaccaata gccagctgga ctggttggga
    18961 tatgggcctg atagacccga ctgattggaa aggcttgatt aaccttgcga tgcatgcttc
    19021 ggcaagctga ctcatcctca tcaaagaagg gactcccatg ctgataaatc atgataccat
    19081 cttcctttag agctcgataa ctattaccgt aaaattcctt ggtaaagagc ccttccgtat
    19141 ggccaaatgg atctgtcgca tcgttgatga taatatcgta gtcatcttcg cagtttcgca
    19201 aaaagcgtag cccattttga taataaatgg taacacgagg atcatctagc cctgcggcaa
    19261 aatctgggaa atactcacga cagacttcaa ccaacatctc atctggttcc acaatgtcga
    19321 tttgttccag ctcaggatag agtgttaata cttgggcaac accgccgtca ccacctccaa
    19381 taaccaatac tttctttgga tttgggtgga cagccatggg aacgtggacg gtcatttcat
    19441 tgtagacaaa atcatccgca tctgaaaaca aaacatgccc atttaaaatc agtattttcc
    19501 caaaagctgg cgtatccaag acttcgatat cctgccattc actttttcca gcgtaaagtt
    19561 gcttggctgt tctcagagac aatttgacat ctggagtatg aacttcagaa aaccataaat
    19621 ccatctattt ctccttatat tatatttagc gcagttagct actgccaaag cccaagtggt
    19681 atacttggaa taagccactg tggattagtt catttcctcc aatcgcttga cgatttccga
    19741 aaggctctaa ccacagc
//
LOCUS       DBEROU010000044        19740 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.044, whole genome shotgun sequence.
ACCESSION   DBEROU010000044 DBEROU010000000
VERSION     DBEROU010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19740
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.044"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(617..1295)
                     /locus_tag="ACL8CL_001707"
                     /pseudo
     CDS             complement(617..1295)
                     /locus_tag="ACL8CL_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(1363..3185)
                     /locus_tag="ACL8CL_001708"
                     /pseudo
     CDS             complement(1363..3185)
                     /locus_tag="ACL8CL_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002917262.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1250 family conjugative relaxase"
     gene            complement(3172..3537)
                     /locus_tag="ACL8CL_001709"
     CDS             complement(3172..3537)
                     /locus_tag="ACL8CL_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HGS1330917.1"
                     /translation="MENRYRTNLKKVFLSDSELGQLKHCIDQSGCQSFSEYARRTLLD
                     PGMNFITIDTTGYQDLVFELKRIGNNINQIARSVNQSQLISSEELQDLKKGIADLIRE
                     VEKEFSIQAKKLREFHGHH"
     gene            complement(3542..3898)
                     /locus_tag="ACL8CL_001710"
     CDS             complement(3542..3898)
                     /locus_tag="ACL8CL_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAG1252 family conjugative relaxosome accessory
                     protein"
                     /protein_id="HGS1330918.1"
                     /translation="MPEQYRDIRKEVNLTANELEMIGVMMKNKGFEHFSSFARNKLLE
                     NELEMTAEKWFTFWRAQKLEQISRDVYEILILSRAEHQVTQEHVSILLTCVQELIQEI
                     GNSIPLSQDFRKKYMR"
     gene            complement(4792..6330)
                     /locus_tag="ACL8CL_001711"
     CDS             complement(4792..6330)
                     /locus_tag="ACL8CL_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HGS1330919.1"
                     /translation="MSDIATQDKNVKKELLEYQGNILILANAGSGKTTFLTKKLKSDS
                     KRLNNYQKLAAITFTRNATEEIKQKLVDIPDNVVVSTIDSFLDKEIILPFLNQKYEIQ
                     TSVQFSFRIDHNFSDYSTGIKQILQDGIFGTYDFPTSKHGKNFKCEVALDILKTIDSA
                     SEYIKYKFNTLYIDEFQDCDQSMNDLFLYLKNELGIPLFIVGDDKQSIYQWRGASPKY
                     INNLWKNKNDFRKKRFIGNFRSLPRIVDFSLALTPEVTVDNIREEGSILYLEPNQDYP
                     KEDIIKYLIDNGDINLQEKNYFLIGINKDIEETATHLNDILSNKVDYVRKNPFIDCTN
                     SNFLQSMAQYYFLEDFSEYDVLNNLFPEYNDEFRKELLKKLKRLRQRPTQSLIEEIAS
                     YLDVSITNYEDKLESQILLEILSNLDNKKMFDTNLMSNNLLMTTHASKGLAADTVVIF
                     VEYLIDWRTGLKFEDHYVAITRAKSKVIIIDNRTIYTSEINRLLSNNNGNFSFEDFIE
                     RRYI"
     gene            complement(6323..8080)
                     /locus_tag="ACL8CL_001712"
     CDS             complement(6323..8080)
                     /locus_tag="ACL8CL_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS1330920.1"
                     /translation="MEFKKLVIENFRNFSSIEVALSNKNVIFGMNDSGKTNLLFAIRY
                     LLDRTIRNKGFIKSDYYRHDTSRPIKIQLELDLSDRKMEGDEESLKSSHSRLLISTVA
                     GARNNSSLDTFFISLEAVYNEKELFGNPVLSWGSTLDNLVEVPQYGTRSDIDKIFQIV
                     YVNPAIELDSFFKRNRRILFSDDSKTDEDVKLEEEIENNIEKLNENISNLHLITSVQS
                     QLTEAYKSYRKENLEIKIQSEISISGYLDNLTPYINWNDDTHNYPTSGDGRKKLLSYA
                     INHLISEKQYNKKVIIYLIEEPENSLHRSVQISLSKQLFVQNIYEYFFLTTHSAEVLY
                     EMDNTQLIKITNQQDATGQSVYYTVPNEFSNLKKKLNKSLAQSIFYDRILLVEGPSEY
                     YLFSAIFEYVSPNFEADGKYLLQVDGINFKPYSELYKALGIDFWIKTDNDLKASRGDK
                     HVFSPIGLNRCIDLLDHSATPILDNYRIDFSSKDERKQNIKSKKEELYLTNHNLVLQL
                     NADGIFISEIDLENDLAQVLSDSERNEMSKLDFVNWLQSSKQYNMLEYISNHLTKEIA
                     LKIVESGYFKVLAEFLDYE"
     gene            complement(8070..8846)
                     /gene="pezT"
                     /locus_tag="ACL8CL_001713"
     CDS             complement(8070..8846)
                     /gene="pezT"
                     /locus_tag="ACL8CL_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin PezT"
                     /protein_id="HGS1330921.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TKFSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSSVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLRVVEERHKELEGKNSYGI"
     gene            complement(8846..9322)
                     /gene="pezA"
                     /locus_tag="ACL8CL_001714"
     CDS             complement(8846..9322)
                     /gene="pezA"
                     /locus_tag="ACL8CL_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PezA"
                     /protein_id="HGS1330922.1"
                     /translation="MIGKNIKSLRKTHDLTQDEFARIVGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            complement(9767..10192)
                     /locus_tag="ACL8CL_001715"
                     /pseudo
     CDS             complement(9767..10192)
                     /locus_tag="ACL8CL_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10169..10902)
                     /locus_tag="ACL8CL_001716"
                     /pseudo
     CDS             complement(10169..10902)
                     /locus_tag="ACL8CL_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511339.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
     gene            complement(11064..11948)
                     /locus_tag="ACL8CL_001717"
     CDS             complement(11064..11948)
                     /locus_tag="ACL8CL_001717"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HGS1330923.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKTFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVVANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            complement(12060..12914)
                     /locus_tag="ACL8CL_001718"
     CDS             complement(12060..12914)
                     /locus_tag="ACL8CL_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HGS1330924.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGIWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCNIAEAAYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            complement(12972..13730)
                     /locus_tag="ACL8CL_001719"
     CDS             complement(12972..13730)
                     /locus_tag="ACL8CL_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGS1330925.1"
                     /translation="MVVYIRQSKLPSEVSINKYNAQVGAYLQGEEVILYQSFYEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVYDYPIELKEFYGRKIYVGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVIF"
     gene            complement(13792..13980)
                     /locus_tag="ACL8CL_001720"
     CDS             complement(13792..13980)
                     /locus_tag="ACL8CL_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HGS1330926.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            complement(14303..14869)
                     /locus_tag="ACL8CL_001721"
     CDS             complement(14303..14869)
                     /locus_tag="ACL8CL_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330927.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPGENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
     gene            complement(15137..16815)
                     /locus_tag="ACL8CL_001722"
                     /pseudo
     CDS             complement(15137..16815)
                     /locus_tag="ACL8CL_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(16907..17068)
                     /locus_tag="ACL8CL_001723"
     CDS             complement(16907..17068)
                     /locus_tag="ACL8CL_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330928.1"
                     /translation="MDNDWNGLADLIANLIAKYAGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            complement(17752..18456)
                     /locus_tag="ACL8CL_001724"
     CDS             complement(17752..18456)
                     /locus_tag="ACL8CL_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HGS1330929.1"
                     /translation="MATNKRYYWIKLKEEFFTDKRIKRLRRISGGDTYTIIYLKLLLL
                     SLKDEGKLYYDGVESDFIKELALTIDETDDDVMVTVNYLINKGLLEVVTENDEYYLTE
                     IPNLIGSETAWAEKKRRYRQNKQRTLSLMSPTHVRQEIDKEREGEIDKKSTPIFYGEF
                     KNVRLSKEEYKNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLR
                     QKGLNKKMNYDVGESL"
     gene            complement(18583..19110)
                     /locus_tag="ACL8CL_001725"
     CDS             complement(18583..19110)
                     /locus_tag="ACL8CL_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HGS1330930.1"
                     /translation="MINEEISKEAGQAAQTIISYTIKATKESINLEKEIRKKMNETLE
                     KANGNLKSLMGDEMKIKDLYKKGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESK
                     NYEIFFQAKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKV
                     KEKVKPNKKDMTREI"
     gene            complement(19296..19469)
                     /locus_tag="ACL8CL_001726"
     CDS             complement(19296..19469)
                     /locus_tag="ACL8CL_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330931.1"
                     /translation="MNKRQELIDELIKANEDGTYKTYKSTEEIKAMNNEEIQIIYSNM
                     KNYLSDKRTHINY"
     gene            complement(19543..>19740)
                     /locus_tag="ACL8CL_001727"
     CDS             complement(19543..>19740)
                     /locus_tag="ACL8CL_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330932.1"
                     /translation="INHIKYSVDFVNKELGIDLSIEIDKLIKQGEKPSVIAQIKKFQD
                     QVNKDNEYREMMKNKKMDQER"
BASE COUNT         5802 a         3869 c         2674 g         7395 t
ORIGIN      
        1 agttgttcac cccttcgctc cacttccact acagaagttt cctcactact atgggctcgg
       61 ctgacttctc atgattcgtt gttactacgc tttcggcgct catgagacct cacgggataa
      121 gccgtacatc tttcctcgtt tatacaagat atgaggacgt taaaaggtta caatgaaaat
      181 aggaaatctg acgcagagtc tttagactct aggaagattt atctttttca catagacctt
      241 agtccgaatt caattactct cgtgatttac gcatataggt tacgactacc tttttggact
      301 ttagagtttt aagccccctt atccgctgta tacgccttac tatcacgttc ctgttcgtag
      361 agcctcgatt tcgctatccc ttcctctgac gattcttatt tcctgcgtca gacttaaaac
      421 ggtctatccc cagaccgttt taatccgcta cctcacgata gtcaggcttg ggaatcgcta
      481 ttgggttcac cggtagcggg tgcccacgga ggacttacac ctcagatgca cgacatgccc
      541 gtcgtacctt aaaaagcctt ggaaaaaact gtttccaagg ctttctctat atttgtcctt
      601 attgaccaaa ccagttacat tgctttatcg tatctgtgat tttagtaata gcctcttcag
      661 tattattctg caaccataga tagcgacaaa cattataatt aaatttaata gaaaggttta
      721 attcttcgat tgtgtttaac ttgagttgat ttacttgata ctctaacttt tctctaattt
      781 cattaaaact ctctaaatct tcaatatcat aataaaaatt gaatagagta tttgcaactt
      841 gagggtaggt caagtcagtt acacttaact gagatagtac cttctccaat attgccacag
      901 cctcctcttt ttgcccatag aaataaaaat ctataagaga ttttatccac tccatataaa
      961 atttatttac cagagacaag cgatgtacct ttacactctc taattcatat atatttcaaa
     1021 gattcataat ttcgatttgt gataaatgtc tgggctaact ttttaaactc tgataactca
     1081 tcaatctctt ctacaatctg ctcattaaaa aaataatcta cactaacttt taacttctta
     1141 gacagagcat ataaaaagtc tgctcctgga gtaaattctc cattctctaa tcgactaatc
     1201 tgtccctgtt tacatatccc ctcagtcaat tctcgctgag acatttttaa ttcttttcgt
     1261 ctatttttta atctggttgc taatagtgtg cccaagtttg cattttcctt ttctattcga
     1321 tttcaaaaga tacaaattat atataagcaa tgactagata ggttattttt gaaaacctat
     1381 cccttcttct tttgaaattt tcttactcaa acgattgata aacttattca atttaaatac
     1441 gatgttctca tatctatcgc tctctaattc tatttggata cctaactcat tcaactcctt
     1501 ctcaacaaca tcaatgccaa ttttatcagt tatattcaac tttgaaagat tcattttcat
     1561 ttcttgctta ttttcaactg aagccagtaa atattctgct acttgattta aggttgtcac
     1621 tcgttcttga aaagcattga ttctcaattc agaagctgct aattcatgaa ctagttcatc
     1681 tttaattgta atataagagt gcttatccgt atccagctca agtaaagtat acacttcctc
     1741 aatcttctcc tttatcatct ccacagttgt tttcctgcgg aacggaattg tttgattttc
     1801 agagctgaat tggcgaatta atgttctacc tttcatatag caatttttct cggcatcatc
     1861 tttgtaatag acatagtagg agctcgtttc tctgataaaa accttcacct tatcctcctc
     1921 catatcgagc tccatgttag gcaaaaagat aagcccttcc tgacgaatac caaacttgac
     1981 cttgatgtaa ataccatcat ccactacttt ttcaatttga ttgagtgaca actctacctc
     2041 aaattcatga atagcatctc tatttctcct gaactcttga taggactccc aaaacttctc
     2101 ctcgcttggg agtctttttc ttttgagaat ctttcttctt gataaacttg aactaaatcc
     2161 tgaacttctg gagctttcaa atcttttctc ttctcaaaat aatctttgaa aaactctaca
     2221 tcatataagt ttttcttgtc tagttctgtt tctttcactt ctactcctgc aaattgaaaa
     2281 gaaacatgct tttgcttagg attgatattt aatccaaata gttttgcttt tttaatcaga
     2341 tcttctaagt tatcaacctt gggtaaaata aattccataa gctcttccat tttccttttt
     2401 gcgaagtaat tcttgaaaaa ttcttctgtg taagcttgct tgtgattgag tttattacca
     2461 cgcaccactt gtctcatagt tgagtccgtc ataaaaaagg tagcatgctt gtgactgaaa
     2521 tccatcttca catgtagtaa tggagcgttc tttttgaaat cctcaaaatc ggtagaattt
     2581 tccatcaaaa aatagagtcg ttgcttaagt tcatacttgt gattggtctt acgataaact
     2641 ttatactgat ggtaagaata gcgattctca ataatttttg cacctgcaat tttagaaaat
     2701 ctatctgaaa tcatgcgaag atttcgttcg actttataat tccactttag ttttttgtcc
     2761 gaattgttat caaccgaatt aacaatgata tgattatgga aatgattttt atcaacgtga
     2821 gtcgcaacga taaaacgaaa tttaccacca gttaactcct tcattgtctc atagccaatc
     2881 tgattgattt gtttaggagt gagatgatct tcgggtgaaa aggactgaat gatatgatga
     2941 gcatgaatta gtcgttgatt ttcctcatgc ctgtcgtggc gaaaatcgta aagcgtatca
     3001 ttactgataa aattttcatg atacatctgc accatctcct cataactagg aaaatccaga
     3061 aaattcctca tgccatagtg cgataccaat gcaagatttt cggtcttctc aggattgaga
     3121 atatacttga taattttttt gcgataacac tttccgtgaa cggcaaaatg cttagtgatg
     3181 accatgaaac tccctcaact ttttagcttg aatactaaac tctttctcta cctctcttat
     3241 caagtcagcg attccctttt tcaagtcttg caattcttca cttgaaatca actgagattg
     3301 attgacactt cgggcaatct ggttgatgtt attaccaatc ctcttcaact caaatactaa
     3361 atcttgataa ccagttgtat caatggtgat gaaattcata ccagggtcaa gtagagttcg
     3421 tcgagcatat tctgaaaaag attgacaacc gctttggtcg atacaatgtt tcaactgacc
     3481 caattcgcta tcagacagga aaactttctt tagattggtt cggtaacgat tttccatgtg
     3541 cctacctcat gtatttttta cggaagtctt gactaagagg aatggaattc cctatttctt
     3601 gaatcaattc ctgaacgcag gttaagagaa tggatacgtg ttcttgggtg acttgatgtt
     3661 ctgcccttga taagattaaa atctcataaa catcacgact gatttgttcc aacttttgag
     3721 ctcgccagaa ggtgaaccac ttttcagcag tcatttctaa ctcgttctcc agtaacttgt
     3781 ttctggcaaa tgaagaaaag tgttcaaatc ctttattttt catcattacg ccaatcattt
     3841 ctaattcatt tgcggtcaaa ttaacctctt tgcgaatgtc tctgtattgt tctggcatac
     3901 tacttcctca cttttatcta ttgtcggata attcccgacc ccgtgtcaac cacgggaaac
     3961 ttataaaaac aaaatctatc atcttgtaag gctttgcgag ctcagatatt gtgtccacaa
     4021 tatcccaaaa atcatatcgc cagctaatca aaacctacca gttttgacag ctaacgataa
     4081 gataacttgg tggctccgcc cccaaacccc cagtgaagat ttgaggtttt attttcttaa
     4141 gaatgatata agaatagcag agaaaatttg gaaaagatat cacaggagaa tgagaggatg
     4201 aaaaaaagag agcaaatgct ctcaatgaac tcatataaat atatatcaaa atactcttca
     4261 aaaacataag gttctaaaga ttatgtagaa aaagctttag caagtcttaa acactctgtt
     4321 gctctaactt atactggtcg atgctaaaat aatttaattc cttgaagtaa ttttccagct
     4381 tttaaatgat actcttgatt tttcatctcc tctaaacgac tatctacagc taaggcaaat
     4441 atattccata ttacattcca tcttcgtatc ttgttcttgt catataatct taaatgtcat
     4501 agagcatgga tttcatattt tgataattag gtattcctac caatgctcga ctagctctat
     4561 ttatcccttc aacataaccc tttgagtcat tcaatatacc aaaattccag aaccttttag
     4621 aattattcgg tgtctagttg taaccgtaat actcgtaaaa tggctaatgt agtacaatca
     4681 tcttcatttt ttatttttca actcctgcac tattaaaatc aatgtgccaa ttaattgcag
     4741 aacctaaaac acctgtaata tcaaattgca atctatcttg atttagacta gtcaaatata
     4801 tcttctttct ataaaatctt caaaagaaaa attcccattg ttgttactta ataatctatt
     4861 gatttcacta gtataaattg ttcgattatc aataattatg acttttgatt ttgctctagt
     4921 aattgcaaca taatgatctt caaatttcaa tcctgtgcgc caatcaatta gatattctac
     4981 aaaaattact acagtgtctg cagccaaacc ttttgatgca tgagtagtca ttaataaatt
     5041 attactcatt aaatttgtat caaacatttt tttattatcc aaattactca aaatttccaa
     5101 cagtatttga ctttctaact tatcttcata atttgtaatt gaaacatcta aatacgaagc
     5161 tatttcctca ataagtgact gtgtaggtcg ttgacgaagc ctctttaatt tttttaaaag
     5221 ttctttccta aattcatcgt tatattcagg aaaaagatta ttcaatacat catactcaga
     5281 aaagtcttca agaaaataat attgagccat agattgcaaa aaattactgt tagtacaatc
     5341 tataaatgga ttttttctaa cgtaatcaac tttatttgaa agaatatcat tcaaatgtgt
     5401 cgcagtttct tcaatatctt tattaattcc aattaaaaaa taattctttt cttgtaaatt
     5461 tatatcacca ttatcaataa ggtacttaat aatatcttct tttggataat cctgattagg
     5521 ttctaaatat agaattgaac cctcttctct aatattatcg acagtcactt caggagtcag
     5581 agctaaagag aaatccacta ttctaggcaa acttctaaaa ttcccaataa atctcttttt
     5641 tctaaaatca tttttgtttt tccataaatt atttatatat ttgggtgatg ctcctctcca
     5701 ctgataaata gattgcttat catctcctac aataaataaa ggaatcccta actcattttt
     5761 tagatacaga aataaatcat tcatagactg gtcacaatct tgaaattcat caatatacaa
     5821 agtattaaat ttatatttta tatattctga cgcagaatct attgtcttca aaatatctaa
     5881 agcaacttca catttgaaat tcttgccgtg tttagaagta ggaaagtcgt aagtcccgaa
     5941 aataccatcc tgcaatattt gttttattcc agtgctatag tcgctaaaat tatggtctat
     6001 ccgaaagcta aactgtacag atgtttgaat ttcgtatttc tgattaagaa aaggaaggat
     6061 tatttcttta tctaaaaacg aatctatggt cgatacaaca acgttgtccg gaatatcaac
     6121 taatttttgc tttatttcct ccgttgcatt tcgagtaaaa gttattgctg ctaatttttg
     6181 ataattattt aatcgtttag aatcactttt tagcttttta gttaaaaaag ttgtttttcc
     6241 cgaacctgcg ttcgctaaaa ttaaaatatt tccttggtat tctaacaatt cttttttgac
     6301 attcttgtcc tgtgtagcaa tatcactcat agtctaaaaa ctccgccaaa actttaaaat
     6361 atcctgattc cacaatctta agcgcaatct ctttagtaag atgattactt atatattcta
     6421 gcatattgta ttgtttactg ctttgtaacc aattcacaaa atctaattta ctcatttcat
     6481 ttctctcgct atcggataaa acctgagcta aatcattttc taaatcaatt tccgatataa
     6541 agattccatc tgcatttaat tgtaatacta gattatgatt agtcaaatat aattcttctt
     6601 ttttagactt tatattctgt tttctctcat cttttgaaga gaaatctata cgataattat
     6661 ctaatatagg agtcgcacta tgatcaagca agtcaataca cctatttaag ccaataggag
     6721 aaaatacatg tttatctcca cgactagctt ttaaatcatt atcagtttta atccaaaaat
     6781 caatccctaa tgccttatat agctctgaat aaggtttaaa atttattcca tcaacttgta
     6841 ataagtattt tccatcagcc tcgaaattgg gacttacata ttcaaaaata gcagaaaata
     6901 gataatattc tgatggacct tcgaccaaaa gaatcctatc ataaaagatt gactgagcca
     6961 acgatttatt taattttttc tttaagtttg aaaattcatt aggaactgtg taatagactg
     7021 attgccctgt tgcatcttgt tgattggtta tcttaatgag ctgagtatta tccatttcat
     7081 aaagaacttc tgctgaatga gtcgttagaa aaaagtattc ataaatattc tgaacaaaaa
     7141 gttgctttga aagagagatt tgaacggaac gatgtaagct attctctggc tcctcaatta
     7201 aataaataat taccttcttg ttgtattgtt tctctgaaat taaatggtta atagcatatg
     7261 ataaaagctt tttacgaccg tctcctgacg tcggataatt gtgagtatca tcattccagt
     7321 taatgtatgg tgtgagattg tctaaatatc ctgaaataga tatctctgat tgaattttaa
     7381 tttctaaatt ttctttacga taacttttat aggcttcagt caactgtgat tgaacactag
     7441 taatcaaatg taagttagaa atattttcat ttaatttttc aatgttattc tcaatctctt
     7501 cctcaagctt tacatcctca tcagttttag aatcatcaga gaacaatatc cttctatttc
     7561 gcttaaaaaa ggaatctaac tctatagcgg gatttacata aactatttgg aatattttat
     7621 caatgtcact tcttgtacca tattgaggaa cttccactaa attatctagt gttgagcccc
     7681 agcttaatac cggatttcca aataactctt tctcattata aactgcttct aatgagataa
     7741 agaatgtatc taagctagag ttatttcttg ctccagctac cgttgaaatt agaagacgag
     7801 aatgtgaaga ttttagtgat tcttcatccc cctccatttt tctatcagac aaatcaagct
     7861 caagttgtat ttttataggt ctactagtat catgtctata ataatccgat ttaataaaac
     7921 ctttatttcg tatagttctg tccaagaggt atcttattgc aaatagaagg tttgttttcc
     7981 ctgaatcatt cattccaaat ataacatttt tatttgataa agcaacctca atagaactaa
     8041 aattacgaaa attttcaatt actagttttt taaattccat aactattttt tccttctaac
     8101 tctttatgtc tttcttccac caccctcaac atctctttct ctacctgact ccactcccca
     8161 aagagtaact cttgaagaac atctgctgct gaagttgtat tttcttttga atcatataca
     8221 gaacttctat ctcgttggta aatttgaatt ctttcaaaga tagccagttc ttccaattgt
     8281 cgtgtattat caactagatg atttacaatg aaatcatgat gttcttttgg agttgcgcgt
     8341 gcttgatttg gattgataat gtacagttct tcataacgga taagagtact tagatacgac
     8401 aattcaggct ttgtcgcaat taaggccaat tgtacttcat atcccttatt tttcaagagt
     8461 tgtgctgttt tctttggaac atcaactgtt cgtaaagttc cctctatcaa aagattgtat
     8521 ctcaaactac taaattttgt tactaaagac tctaccattt ttcctgcaaa atctttggta
     8581 tattctacac tgtctttgcc atattcttgc tgcagttcta aatagtgtgg atgctgagaa
     8641 cgaaaactat caccatctat gataacaata tttccttgaa attctttctg tttaatacga
     8701 tgaattgtag tcttaccggc accactttga cctccaagca aaatcgctat aggttgctta
     8761 ctggactttt ttcctcttgt cagtgaacga agattccttg ctaaagcatg tttgaattca
     8821 ctatcagtat aatcttggat ttccattaag ctaccatccg cttttcagat atctctaaca
     8881 tacgttcaat tccatccaaa tagccactat atctctctat ttcatcaaaa gtttctacta
     8941 aatagatatt cgtatgaatc aaatcagaaa gatcatcact cattaaaatc caaggattag
     9001 attcatcatc aatgctaatt ccctgactat cttgatagcg atagagtcga gataatagat
     9061 tagcacctct ttctttcaca atttcaattt ttaaagtcaa ttcataatct tcaacaggat
     9121 tgagcatttt atcttctcct acgatatcga cataagatac attaaacttc tgacaaatga
     9181 tgtctattaa ttcggtagag actgaactcg ttccattttc ataacgactc agactatttc
     9241 gtgaaatacc tacaatccgt gcaaattcgt cttgtgttaa gtcatgtgtt ttacgtaagg
     9301 attttatgtt ctttccaatc atggcaaact cctttatttt tataattcaa ttatagcata
     9361 aaaaagcata atgcaccaaa aatggtgcat tatttcttgt ttttctatcc taaactttac
     9421 ttctataaaa gtcacctaaa gagccataaa tatttattct tgtattaatt ttttcgatgc
     9481 gcaaacgata tctacaaata catctataac gattcacctt ttaggatttt tattgtcaaa
     9541 attgccttta tctacaaaga caaaatgctt ataaaatagc ttgaaatcaa ggattatcac
     9601 ctttttcgtt gtagcaacac tccacatgtg attattaggc tattagcaaa gcgtatccaa
     9661 tacctcaata aagtgttgtt tttactgata ttttaactat attgtatagt ctactattta
     9721 aatctcaatt aggatatttt atagaatacc caaaattcaa caataactaa aagtaagatt
     9781 ccattgtatt tccttgttgg tctttcatat cacgaatttt atttttcaga atattaaagt
     9841 tacaaatttc atctctaata tcgttatccg ctctgtagcc attaatcatc tcagtcagag
     9901 ttttcccttt actaccattc tttatagtag tatttactct ttttatactg ctttcaaaag
     9961 gctcatatgt tttatactta attacttcgt ctttaaaatt aaataaaggt gtatccggct
    10021 tagcattcca taaaataatc aaccacatta tagcattata aagctacttt gcagtttcat
    10081 tccacttttc aggttgtaaa tttccactcc ctgtattgtc atattcttgt ctattattta
    10141 catgagaatc acctacaagc ggattaaaaa gaggtagccc tttgtgatta attagttcta
    10201 aaaaatccct tgccgcaccc atgcaacact gtctccaaaa tgacggtcaa tttcaaagta
    10261 atctgtcgta ttgtacttgt gattgcttgt agattcaaag atgggacaaa gatatagtcc
    10321 agtaataccc aagtcttgca agtaatccat atgatcaata atcccctgta aatcaccacc
    10381 aaagaaatca tcgctcttag gtgtgacaga tgaatcccag tctaaagtcc cttctgggtt
    10441 taatagagca ttgccattgg caaatctttc aggaaatatc tgataccata ccgtatctga
    10501 aacccagtca ggaaccttgc aggcatcaat ctcatgaaga taaggcaact taaatccatt
    10561 cccaattgca tgaagatttt ctagagaatt ttccacacac cctttatcgc catacaaaat
    10621 attttgacct tctgtatctc tgagctcaaa gagatactgg atacgtgcaa agtcaactga
    10681 cacttcaacc tgccaatggt caaataaggt accagaagtt atcttgacca tttcttttgt
    10741 atcctgataa aactcctcca taaagataaa agggtcccca tagtgcaagt taatgctttc
    10801 aatgtcccct ttcttagttc gaattcgaat atggagtttc ttatccttat aaagataggc
    10861 atactccgac tcaggcctat ggtaaatagc actcaattcc atcaatttgt ctcctaaagt
    10921 attcattcaa attttacgca aacgttttca aactcaataa atccagtata ctatttttgt
    10981 aaactattta caaggaatgt ccgttgttaa aggaactatc ttttaaagta tacaaaaagc
    11041 cttaaaaagg ctttctctat atactaatcc tctttcggtt ttgaaaatac ttctactcgc
    11101 tgagctagga ctttgatttc tacaggtagg ttatctgatt tatctccgtc aacatctgac
    11161 tccaattcac tatctgaaga gattttaata ttacgcgctt tgatatactc gatattatca
    11221 tttgcgacaa catccccttt taataaatca ggaatgacgg ataatttgga gaatatagag
    11281 gcatctttca gaatcaaaat gttggcatag ccgtccttgt tttcttcaaa ggttttctta
    11341 tcagcgaagt aatttgtcaa aaggaccaaa acatggctag cttcaccaac ataatttcca
    11401 ttttctgtct caaccttaat attaaagacc tgatctgtca tgacagactt catggtattt
    11461 acagcatagg ttaaaatacc gaattttgtt ttgtcctcta tttcaacatt gtgaatcgcc
    11521 tcaggcagag aaccgatact aaagatataa ccaaaatagt tgtcatttgc tttaccgata
    11581 tcaatcttat tggttaaatc aaaatccagt tcgtcaattg cgccatcgat gtcttgattg
    11641 atttccaaaa gttttgtaat gaggttaccc gtaccgcctg ggataatccc taacttagga
    11701 atgtagtctc tctcatcaat acctgaaatg acttcattga cagttccatc tccaccaaac
    11761 acaaccactg catcatactg ctcacgagaa gcttcttcag caaaatgtgt tgcatccagc
    11821 gctttttcgg taattttggt ttcaacatat tcaaagtatt cttttgcttt attctccagc
    11881 ttttctttgt aatccaaagc cttctcgcca ccagaagtag ggttgataat taccattgct
    11941 tttttcattg attttatcct taattttaaa cagaaatgtt tacatttcgt cgtatgcaag
    12001 taaatgtaat cctattatac aatgaaaata cagaaaagag aaatctgacg tactggagat
    12061 taatacgctt ttattctatt ttcccatcgc ctaactacat cctttaaggg ttcatccaag
    12121 taagaatagg ccttatcctt gatccaatca ggaataccgt aagctgcctc tgctatgtta
    12181 caagtgattg ctgcgagagt atcactgtcg ccaccaagtg agatggcatt tcttatcgca
    12241 tcttcgaagt ctctactttc aagaaaggcg ataatggctt gagggacagt ttcctgacat
    12301 gtttcgttaa aacgatagtt aggacggatt tcatctaaag tttgagatag attgtaatcg
    12361 tattcttttt ctatataatc cttaattcgt cttttacact ctgtaggatt gtcgttaatt
    12421 gattgctcgt actctctaca ataaccacca aagtaaaaac gacatagaaa gatagcatca
    12481 gctgtagcca tagcgccttt gacaccttct ggatgattat gagttacctc tgcagaaaga
    12541 ctcgtaagtc ctctagatga tggccatata ccagttttcg cataaaaacc acagtccatg
    12601 atccaagcac atggagaaat acgcatagct gatccattac caaagctatt ataaggctca
    12661 cggttatcgc tgtttagcca tgcattaaac cgagcaccgt aatcagcatt cggatacatt
    12721 ctgccatatt tcttcatcgc gtcaatgaag tcgtcttttt gtccaccatt cataattgct
    12781 tctgcaacag cacaagtcat aaccgtgtca tctgtaaaaa agcagtcctt ccgaaataaa
    12841 ggaaagtcct ttgttttgat attgttccat tcgtaaacag aaccgacaat atctccaata
    12901 attgctccaa gcatcagatt cctccttgtt cattttgatg ctttttatat tggttatcta
    12961 ccatatttat tttagaaaat aacatcctgt tggattttaa aaatttcatt tttttcaaaa
    13021 tagggtttta ccatttcttt ccacctagct ctatgaaaat tgattgattt taaaggagat
    13081 aggccataat ttcccaatgc ataaccatca tttacttcaa caacaagtgt tctgccatcg
    13141 cgagtaacac cgatatctag tccataagct attggcgcat ctttccaaca tgatatcgct
    13201 tcatcaatta cacttgcatc aaattgtgca tgataatcac ctgtataggg tcgaacatct
    13261 aatacgcgac catctaacac aaaacaacgc cattcagcta tgaattctac aacctcacta
    13321 atccatatag gatagtcgaa aggtagacca atacctatta aatcatgggt tccattaaca
    13381 actcttccag taaagacttt tgaaccagct ttaggcttaa taaattttcc ccaattatca
    13441 ggtatattca caatctctcc taaaatacca acataaatct ttcgaccata aaactcttta
    13501 agctcaatag gatagtcata aaccggaacg tttaagccca tcatttttag taatgctcta
    13561 gtctccatta tataatctac aactatatct tcacttgtta actcttttat ttcataaaaa
    13621 gattgatata aaataacttc ttctccttgt aagtaggcac ctacttgagc attgtattta
    13681 ttaattgaaa cctcacttgg taatttactt tgtctaatat aaacaaccat ttcatcactc
    13741 ctgtatcact agtgttacac caatttgtaa aaaataatag caattttgct cttatttttt
    13801 tgagtaaata gcccccataa tatcatcgaa ataatcaacg gtatttagga gtaattcaat
    13861 aacctgggac tttgttagtc gcattcccct tctatctcta gcatcttcta ctaaattttc
    13921 aagtttctct agatttttat catccaagct aatcattatt ctatttttat cggttgccat
    13981 tttcatcacc tcaagttaat tctatcacag gtgtaacact tgtgttaact ggcttttata
    14041 atacattagt ttaaaagtgg agaggatttt ttacacagta actttaaatc tttggtatta
    14101 aaaaattttc acaatattta tagaaataaa atctgtctca aatcagttat caaatctagt
    14161 ataaattatg agcggctact ctaatacttt ccctctaaac aagaaaaaga cttacactca
    14221 aggattttct tcccccccct tcgttataac gttttgactc ttttactagc aaaggtatat
    14281 actcacaagg aactttggtt gactattgaa tctctccaac ttcttcttta acatatcctt
    14341 ctacatcttc aatctctaca aacattgggt ctaagtgaca caagaaatgc caaacttcga
    14401 tccctttttt tctgtaaaga atcgcttcac cgtcttcact tccgaaaaag cttctgtcga
    14461 tttcatatcc gcggctttct aagaagtctt ttgctttacg atagttcgtt tctcttgttt
    14521 cgacataggc tttaacttca tggttgttaa cgacatatgc atcaattttt gaatatcctt
    14581 cgatcactct atcatttttg agggataaat ttgaaatctc tttccaaata atgtttacat
    14641 tttccccagg atcgaacata aatttagata aaggaacaat atttccgtta aaaataattt
    14701 ccatataatc cggtatgttt ttaggattaa aatactccac ttcaaaacca tcttctgttt
    14761 ccagagtgta tcccgggatt tgagctacaa aggctttccc atcttctatg gaatcaaatg
    14821 ctactaaatc tttagaataa tcattttggt acaattccaa tataaccatc gataatctct
    14881 ccattttcat tatcaggcta atgtaaataa gcacgtcacc tgaccaattc aggctctctg
    14941 tatcatctca tcatatttcc tacttacttt acgagtccta tacccagaac acaccttatc
    15001 gaccttcggt ctcacctcgt tgcattggct gaacatctac ttttacttcg ctgatgcttc
    15061 aactcgtaca agcagtgata ccgcctcagc gtgatgcgtc agtgggactc aaaaggttcg
    15121 gggaaccttt tgaggattaa ctacgtttct ctaataaact tacacattca acttgttcat
    15181 cattgtccaa acctatgttg agattttctt ctataattgg tagcttaaaa gtaatggatt
    15241 ttagccattg tccgttagat tgtttttctt cataaacttg aatttcagaa atcaaagctg
    15301 aaattaactg cctacgctct acatcattca tgactttata gagcttatca aaatagatca
    15361 gaaccttata tatgttatct cctgtaagct tttcagcttc aatagtctgt ttctttgctt
    15421 tcgcatcaat tagtgatgat tctaattcat ctattttgtc atacatacga tatagtctat
    15481 catctaaatc ctgtttcctt ctcttatagt gcttatcttc aacatctaaa ttatctattt
    15541 cctcaattag cttaaacttt gtagaatgac tctttctcaa ttccttttgg taattatcta
    15601 tttctttttc tatttcagag gtatccacct tcatgttgat tttttcttgc atcatagaag
    15661 caaatttcgg attacttact atcttgacaa tcacctctgc aacagcatca tctaacaatt
    15721 cttctctaat ttgcttactg aatgtacact tatgacctct tatcatctgc ctatgtttac
    15781 aaccatagta ataaaaatct ttatactttg tgccatcttt ctttttcttg atacacttgt
    15841 tcccaaacat tcccactcca catatccggc attttacaat tccagaaagc aagtgtgtgc
    15901 gtgtatcttt tcctttattc acatgctcat atttctttgc ttgagatttt agcttaacct
    15961 gagcagcttg ccaaacttca tcggaaacta tagcttcatg tatcccttca gatattagat
    16021 attcatcttg ttcaacctgc ttatattcat ttcttgtacc atgaactttt tctaaagttc
    16081 ttcttccaaa tactattttc ccattatata caggattctt taatatcttt cttataagac
    16141 ctgcatcaaa caaaggattc ttaccattct gtcttgggat ttttctaatt ccatgattct
    16201 ctaagtattt agatatccca ttggctccta tcgtagtatt tacatactgg tcgaaaatcg
    16261 ttcttattgc aactgcctct tcctcattta taaacagctt gccgtcttca agtttatatc
    16321 catacggagc aaagccacca ttccattttc cttcccttgc ttttgaatgc gaccttccat
    16381 tgtttgaata cggatgtttt ctctttctat ttcagccaca gctgataaaa cagaaatcat
    16441 tagtttccca gcatctttag atgaatcaat gccatcttca acgcagataa gattaactcc
    16501 ataatcctgc attatctgaa gtgtagaaag aacatcagcg gcatttcttg caaatcttga
    16561 taacttaaac acaagaacaa aagatactcc atcttttcca gattttatat cttccatcat
    16621 tcgattgaac tgtattctac cttcaataga cttgccagac ttcccggcat cttcatactc
    16681 tccaacaatt tcataatcat tgtaaatagc aaaagctttc attcgtgatt tttgtgcctc
    16741 taacgaatac ccctctatct gtattgacgt agatactcgt gtatagaggt atacttttat
    16801 tttttctttt gacatagtat taacctcaat ataatttttc tatatcatat ataatttttt
    16861 taatttaagt ttggactatc atttcaagta tattataaca cttttattag tccgtctcaa
    16921 tttgtgtttt tgccatgtca aaactatttt tcatctcttg attttttgct ggcgttggat
    16981 cgggtagatt atctaaatct aaagcaccag catattttgc aatcagattt gctattaaat
    17041 cagccaatcc attccagtca ttgtccaata tatacctcct ctaaagtttt atatctaata
    17101 attatttgtt taattaagtt ttttgacatt gacaagtgct ttggattagc aacataggaa
    17161 tctcacctcc gcctctattc cggatgagcc ggcttcaacc ttagaagtat cattatcctc
    17221 attttcttca tagcggatag ggtatccctc cctatattca aactcttact tatcgctcac
    17281 tttctttttg ctcagcagaa ctttttttgc cgaattattc agccgaaaga tcttgacgga
    17341 taggttatta cgctccaaaa ataattaacg tcttgtcttg gtctattcaa ttgttaaggt
    17401 tcaaaattta tcgagagtta ttaatctttt taaaatttga ccatcagaaa atatttatct
    17461 tgatgtaata aaattctata aattaccctc ttatacttaa cagtgaaaag aagtctttct
    17521 tggtaaccaa ttttgaaata gaatttgctt atataaaaag gtccaattcc tactgcataa
    17581 gtagcagtga aaattagacc ctcttggtaa ctgtcatcta aaagtcttct agcatttctt
    17641 taattttcta atcgccttta aagtgtcata tttatatttt tttatctctg gcatactttg
    17701 ataccttttt agacttaaag tctttaatag tgcctttcca cctcttttta tctataaaga
    17761 ttctcctaca tcataattca tttttttatt taaacctttc tgtcttagtt tgtctttatc
    17821 ttcttcatac cattttaaga ttgtcacata gtggttttga taggtcttac cactgctttc
    17881 catgtatctg gatagtttat ttatcattat atctgtgtgt gagtttaatt tttcttttag
    17941 atttttatat tcttctttgc ttaaccttac atttttgaat tctccataaa aaatgggggt
    18001 ggacttttta tctatctctc cctctctctc tttatctatc tcttgtcgga catgggttgg
    18061 actcattaag gacaatgttc tctgtttatt ttgtctgtat cttctttttt tctctgccca
    18121 ggctgtttct gatccgatta agtttggtat ttcagttagg tagtattcat cattttctgt
    18181 tacaacttct aataagcctt tattgattaa ataatttact gttaccatta catcatcgtc
    18241 tgtttcatct atggttagtg ctaactcttt tataaaatca ctttctactc cgtcataata
    18301 gagtttgccc tcatctttta ggcttagtag tagaagtttt aggtagatta tcgtataggt
    18361 atctcctcca gatattctcc ttaacctttt tatccttttg tctgtgaaaa attcttcttt
    18421 taattttatc caatagtatc ttttgtttgt tgccatatct gctcctttct tgattttcga
    18481 taatcgaaat tcttgatttc cgattttcgg aattctggac ttctgttttt cggaagtctt
    18541 gttattatgg ttatttaagg gtgtaacttt tcgttactcc ctttagattt ctctggtcat
    18601 atcttttttg tttggtttta ctttttcttt tactttcctt tttccttttt cttttgtctt
    18661 atctttgttt ttggataggt ctttgtattt ctttatttgt tttaggatac tttctttttc
    18721 ttttttattt tccttggcta tgacttgctt aaaggcatat tccattactt ttatgtcttt
    18781 ggcttggaag aatatttcat agtttttgct ttctttgttt ttcattactg agaaacttac
    18841 tccaagtttg tttagttctt tttttaagtc tttgaggtcg atttgatcta tgcttatatt
    18901 ttctagttgt cctttcttgt agagatcttt tattttcatt tcatcgccca taagactttt
    18961 taagtttcca tttgcttttt ctaaagtttc attcatcttt tttcttattt ctttttctaa
    19021 attgattgat tcttttgttg cctttattgt gtatgatatt atggtttgtg ctgcttgacc
    19081 tgcttccttg cttatttctt cgtttatcat gcttttctct cctttgtttg aattaaaaaa
    19141 gggaagtata ggttttaatt tttctatact tccctttgct tgtttttatt ttattgttct
    19201 tggtggtgga atactttatt ttgatgtttt tatatatttc ttaggtcttt gtacctttta
    19261 tacttttaaa aatagcttac actacctttc cacctttaat agtttatgtg tgttcttttg
    19321 tctgatagat agtttttcat gttggaatat ataatctgta tttcttcatt gttcattgct
    19381 tttatttctt ctgtgctttt ataggttttg tatgttccat cttcatttgc ttttatgagt
    19441 tcatctatta gctcttgcct tttattcatc tctattacct cctagatctg actttagttg
    19501 tttggtcata ttatagcttg gataagatat ttttaccagg tcttatcttt cttgatccat
    19561 tttcttgttt ttcatcattt ctctgtattc attatcttta ttgacttggt cttggaactt
    19621 tttaatctgt gctattacac ttggtttttc tccctgtttt attagcttat ctatttctat
    19681 ggataaatct ataccaagtt ctttgtttac aaaatctact gaatatttta tatgattaat
//
LOCUS       DBEROU010000045        19254 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.045, whole genome shotgun sequence.
ACCESSION   DBEROU010000045 DBEROU010000000
VERSION     DBEROU010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 19254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19254
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.045"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            87..3437
                     /locus_tag="ACL8CL_001728"
     CDS             87..3437
                     /locus_tag="ACL8CL_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HGS1330933.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIDQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQALLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDQEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSEEEKEHFD
                     SKFEDNSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNRAGEAA"
     gene            complement(3942..4412)
                     /locus_tag="ACL8CL_001729"
     CDS             complement(3942..4412)
                     /locus_tag="ACL8CL_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HGS1330934.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAKNDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(4429..6702)
                     /locus_tag="ACL8CL_001730"
     CDS             complement(4429..6702)
                     /locus_tag="ACL8CL_001730"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HGS1330935.1"
                     /translation="MRFNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRF
                     FFTYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDV
                     FRKETGFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLAT
                     FSNQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKA
                     SDKALYKMEVELEVKLPHKIELEEPQLNLVQPQGQAELVAEAEEKLTHINSSYTLNDY
                     FLPRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVK
                     ADWSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGG
                     YPGEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYL
                     LNAHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSID
                     FRESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCKTFSLGQEEQ
                     AIQNQYPDKDFERYSKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSS
                     TNKGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKG
                     YLNLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNT
                     DYHLTVDLAQSMLTLPC"
     gene            7048..10149
                     /locus_tag="ACL8CL_001731"
     CDS             7048..10149
                     /locus_tag="ACL8CL_001731"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HGS1330936.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGVK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQECFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRDNLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLA
                     ETEGIHLKIEEIDLEDKETLALFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDPVLEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSLEAGHTVEKAEQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSSNSDYLIDALEAGFEVASLSINTIPYH
                     DKIANKAIYLGLKSIKGVSNDLALWIIENRPYSNIEDFIAKLPENYLKLPLLEPLVKV
                     GLFDSFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVQKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQLRDGRLQMIAQEIREAVAERFWIQ
                     VKNHDSDQEMSRILDQYKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNGIVMKT
                     IYR"
     gene            10231..11238
                     /gene="pfkA"
                     /locus_tag="ACL8CL_001732"
     CDS             10231..11238
                     /gene="pfkA"
                     /locus_tag="ACL8CL_001732"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HGS1330937.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEAGFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPYKADIELSSLNKSL
                     S"
     gene            11295..12800
                     /gene="pyk"
                     /locus_tag="ACL8CL_001733"
     CDS             11295..12800
                     /gene="pyk"
                     /locus_tag="ACL8CL_001733"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HGS1330938.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDATREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATNSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAIEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(13019..13372)
                     /locus_tag="ACL8CL_001734"
     CDS             complement(13019..13372)
                     /locus_tag="ACL8CL_001734"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HGS1330939.1"
                     /translation="MTLQKANTKLEKPINQPLSSHIFRHTLLSTLAEKNAIMVRVEHK
                     NAKTVNNIYTHVSKRMEQATLEVLNTISLNRKYIRSNLDKYITIAKTFVEIHLNFLIN
                     LCISYFILNENQRAN"
     gene            complement(13542..13757)
                     /locus_tag="ACL8CL_001735"
     CDS             complement(13542..13757)
                     /locus_tag="ACL8CL_001735"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HGS1330940.1"
                     /translation="MEPKIRNSDYLFIKNTPQIDYNTIGIFQVDGTNYVKKLRQGYLK
                     KTQSRILRHPPRRKQPYSYHRESCQAI"
     gene            14605..15477
                     /locus_tag="ACL8CL_001736"
     CDS             14605..15477
                     /locus_tag="ACL8CL_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HGS1330941.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLDTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(15626..15966)
                     /locus_tag="ACL8CL_001737"
                     /pseudo
     CDS             complement(15626..15966)
                     /locus_tag="ACL8CL_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032196 - transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            16109..16420
                     /locus_tag="ACL8CL_001738"
     CDS             16109..16420
                     /locus_tag="ACL8CL_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330942.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEYPELYAVAQTSGEIPEKEREELRQIITTIFEQKMNKHSI
                     K"
     gene            16424..17140
                     /locus_tag="ACL8CL_001739"
     CDS             16424..17140
                     /locus_tag="ACL8CL_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HGS1330943.1"
                     /translation="MKKYFISGLGSNAYHSKDFFQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEESICLIGHSLGGDLARYFASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVSDLALLISKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNGATWREEALKELPDYFNTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(17270..18085)
                     /locus_tag="ACL8CL_001740"
     CDS             complement(17270..18085)
                     /locus_tag="ACL8CL_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HGS1330944.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQITNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(18082..18987)
                     /locus_tag="ACL8CL_001741"
     CDS             complement(18082..18987)
                     /locus_tag="ACL8CL_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HGS1330945.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSILVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSYLSSAKKVFQVFVQAIDEFFDTGRYLVFGCLFASIIQVYVPTRILTSISATPLFA
                     ILLLMLLAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            complement(18988..19119)
                     /locus_tag="ACL8CL_001742"
     CDS             complement(18988..19119)
                     /locus_tag="ACL8CL_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPJ_0845 family protein"
                     /protein_id="HGS1330946.1"
                     /translation="MAVKFTKRDDLDKMFEEFAKLPDLKQVTFPDDKEKKVKAEKKN"
BASE COUNT         5987 a         3302 c         4111 g         5854 t
ORIGIN      
        1 gcttgtgaga tagctataaa agtgtggaga aatagcttga aatttagtat aatatagcta
       61 aactatttgt ttaaagtgag aaaaaaatgg gaaattttag ctttctttta aaaaatgacg
      121 aatatgaatc tttttcaaaa ccttgcattg aagctgagaa tatgattgct acatcaactg
      181 tggctactgc ctttatggcg cgtcgtgctt tagagcaggc tgtccattgg atatatagtc
      241 acgattcata tttagaagct ccctatcgtg ctactctatc ttctttagta tgggatgatg
      301 attttaggga tatcgtagat tctgaactcc acaagcagat agttctgttg attcggtggg
      361 gaaaccatgc tgctcatggt ggtgaaatta aggaacgaga agcgatttta gctttgcatc
      421 atttgtatca gtttgttaat tttatcgatt attgttacag caatgagttt gtggagcgtt
      481 attttgatga gaagtgctta ccactttcag caaacatcaa ataccgagaa actccacaat
      541 ctatgataaa gttacaagac agtttaccag aactgcctga ttttcatgaa cagatggctg
      601 ctcagtccgt agaagttcaa gagacttata ctgaaaaacg tgagactgca gcgcaacggc
      661 aagatgtgcc tttccatatt gatcaattat ctgaggcaga gacaagaaag ctctttattg
      721 atatcgatct ccgtttagca ggatggatat ttgaagaaaa ctgtcgtgtt gagatagccg
      781 ttgatggtct caagcacggt tcaggaattg gttactgtga ctatgtactt tatggtaaaa
      841 atgggaaaat tttagcgatt gtggaggcta aaaaagcctc tgtcaatcca gaagtagggg
      901 aagtacaggt caaagaatat gctgaagctt tggagaaaca tatcggctat cagccaattt
      961 gctttattac aaatgggttg aagcactata tacttgatgg tccgaaccgc cgccagattg
     1021 caggctttta ctctcaagaa gaattgcaat tagtgatgga tagacgtcat cttcaaaaac
     1081 cgcttgagga tatttctagt aaaattaggg acgatatttc cgggcgtcac taccaaaaac
     1141 atgccattgc aagcgtttgt gaagctttct ctaatcatcg tagacaggca cttttggtta
     1201 tggcaactgg ggcggggaaa actcgtacag cagtttctct agttgatatc ttatcacgtc
     1261 ataactgggt aaaaaacgtt ctcttcttag ccgatagaac ttccttggtt aagcaagcct
     1321 atgattcgtt tagaaaatta ctcccagatc tttccgtttg taacttctta gaagatcaag
     1381 aaggagctca atcaagtcgt atggtctttt caacttatcc gaccatgatt ggagcgatta
     1441 gtggtcaaga agaagtaaat caacgccctt ttactgttgg gcattttgac cttatcataa
     1501 ttgacgaatc tcaccgttct atttatcaga aatacaagtc catttttgat tattttgatg
     1561 caagaattgt aggcttaaca gctactccgc gtcaagattt agataaaaac acctatggat
     1621 tctttaattt ggagaatggg gttccaacat atgcatatga tttggaagag gctgttaaag
     1681 acggatattt agtagcctat cattctatcg aaaccaaact gaaactacct acggatggtc
     1741 tacattatga tgatttgtcc gaagaagaaa aggaacattt tgatagcaaa tttgaagaca
     1801 atagctgtga aaaagatatt gatgggagtg tatttaattc ctttattttc aataaaagta
     1861 cagtagaaat tgttttaaat gaactcatga caagaggaat tcagacagcc tcgggtgatg
     1921 aaattggtaa aactattatt tttgctaaaa atcatgacca tgcggaatat atcagaggta
     1981 tttttaacaa ccgctatcct gaaaaaggga gcgactatgc tcaggtgatt gattatagta
     2041 ttaagcatta tcagaccttg attgatgatt ttaaaattaa ggagaagtat cctcaaattg
     2101 cgatttctgt cgatatgtta gatacaggta ttgatgtacc agaggttgtt aatttagtct
     2161 tcttcaagaa agtacgctct aaaactaagt tttggcagat gattggtcga ggaacccgtc
     2221 tatgtaaaga tttatttgga cctgagcagg ataaggaaaa cttcttggta tttgattatg
     2281 gggacaattt tgattatttt cgtgcagatc caagagatgg agagggtcgt cacattgttt
     2341 cgctgactca gcgtttattt aatatcaaag tggacttgat tcgagaactt cagggactcc
     2401 aataccaaga agatcagttt gcgagagcat accgtcagca gcttgtctcg gaacttcaag
     2461 gtcgtataga gagcttaaat gagttggact tcagggttcg tatggtttta gatacagttt
     2521 atagctatag gaaattggaa agttggcaga atctaactgc tgttacaagt gaaaccattc
     2581 aaaaaaatct ctctccgctt ttatttgatg aagataaaga agatgagatg gcgaggagat
     2641 ttgatttgtg gttgcttcat attcagttgg ggcaactgac agctaaatct tccactgttc
     2701 atatttccca agtgatgaag acggctagag ctctttctgc tattggcaat atcccgcagg
     2761 tttttgagca ggctgaaatt atcaggaaag tacaggagcc tgaattttgg aaagaagtta
     2821 acttgtctga tttggaaaaa attcgtcttg ctattcgaga tttattacag tttttggata
     2881 aaacagaccg taaaccctac tatgttaact ttgaagatcg tatactctcc actgttcacg
     2941 agaccacagc atttttgcag gtcaacgatc ttcggtctta caatgaaaaa gttgagcatt
     3001 atttgaaaac tcatctggat gaggagtcca tttctaagct ataccataat aaaaagttga
     3061 catctgatga tatgcttgca cttgaaaaat tgctctggga aaaattaggt agtaaagcag
     3121 actaccaaag tcattatgaa aataaggcaa ttccgagatt ggttcgtgag attattggct
     3181 tagatagaga gtctgccaat cgtatttttt ctaaattttt gtcggatgag aatcttaatg
     3241 ccaggcagat ttcatttgta aaattgattg tagactacat tgtagaaaat ggttttttag
     3301 agacgaaagt gttaacgcaa gagccgttta aatcttatgg ttctgttcaa ctactcttcc
     3361 aacaccaact accagtactt cgtaatattg ttcaaatcat tgaacttatc aataatcgag
     3421 ctggagaagc ggcttaaatt ctaaagtgat tgccatgctg agactcattt aaaattaaaa
     3481 agagtagaaa tttatgctat atatgagaag ttttattagg aagaatgtca tcgttttcct
     3541 agaatacagt atcagttgtt aagtggttga taaatttcaa agtagatact tgtaccacga
     3601 tgtttgttga tcgagttatt aacaaaagag ctactttgat tttaaagaaa tagaaaacaa
     3661 aaagctgagc aagaattcaa ttgcaggaga aaatgaaata atactcaatg aaaatcaaag
     3721 agcaaactag gaaactagct gcaggctgct caaaacactg ttttgaggtt gcagatggaa
     3781 gctgacgcgg attgaagaga ttttcgaaga gtataaatct tcctaggata aagcaaaacg
     3841 catagtatca agggttttca acacttgata ctatgcgttt tctgatgtta aagactttct
     3901 accagttttt tttaagcata attgttagtt gtagtcattt attattcttc aaagaaaaat
     3961 ggtggggcga attttttcag ttcttcaaag cacttttgag cagtatctgc atcttcacag
     4021 atgataagac agacatcatt accacaaagg gtagcgatag cgtcagggaa gctcaaagta
     4081 tcaatgatag aaccaaagga ttgagccagt ccaggaaggg tttttagtag gacttggtgt
     4141 tgaactgggc gcatccagac aagggcgtct tccatgtaga gttcgagacg tttttcccat
     4201 tttgagatgg aaccattgtt aagaacataa taagcgctat cttcttcgcg gacttttgat
     4261 aggttcatat ttttgatgtc gcgtgagagg gttgcctggg ttacttgaat gtcgttctta
     4321 gcaagaaggg cttgcaactc agcctgtgta tgaatcttgt tttttgtgat aagagcgcgt
     4381 ataagttggt ggcggtgttc tgatttattc ataataatgt aactcctttt agcaaggtaa
     4441 ggtaagcatg gactgagcga ggtcgacagt caagtggtag tctgtattgt cacggatggt
     4501 gatttcaaag tcagtagtat agaggactaa acggagagtg tctccttctt ttagcttgta
     4561 aatagttggc tgcagttcaa attgaacatc catccattca tctgcagtaa tatcctctac
     4621 taacagtaaa tcatttctat tttgtaaatt aaggtaacct tttgtcacga ctcgttgtgc
     4681 ctctggtcta aatggcaatt cacagagatt ttccaacatg tgatagcgac cgttgtcaat
     4741 ggttctagca cttaaaatag ctggataagg ttgtaggtat ttcttttgcc caaattctag
     4801 cagttgggca gataagagcc ccttgtttgt actggatttg atacgaagat tgagctgagc
     4861 gcgaccgttt aggtggagat ctttagtcac aggaaggtta atagtaatct gattagcttt
     4921 cccttgatag agctctgtat tgaaggtttg gtatgtctta ctatagcgct caaaatcctt
     4981 atctgggtac tggttttgaa tagcttgctc ttcttgacca agtgagaagg ttttacagtt
     5041 ttcttgccca ccgaagttat caagtgataa ccaagtctgt ggagctgtat tgtcctgcca
     5101 gataacagta ggaagttgaa agtctgtttc ctgtcctagt aatttcttgg ttaataaggc
     5161 atttatggac tcacggaagt caattgattg ccaattgttc atgtaaacat gggcaccatt
     5221 atggaaaaag agatgcttgt gtatatgagt aggaagagca tggaacatct ggtaaacatg
     5281 aagtggtttg acattccaat cctgagaacc atgagtaaag acaacctctg cctttacttt
     5341 atgggcattg agcagataat tgcggtcatg ccaaaactga ttgtagtccc cagtttttcg
     5401 gtctagctga gctttcactt tttctaagtc agcttggtga gcttcattgc cacggatata
     5461 gtcgccagct aagagattac gagaataggt taactcagca agggagtcaa agtcctcacc
     5521 tggataacca cctgggctag tcaccagacc gttttcacgg tagtagttgt accatgatga
     5581 aattcctgcc tcggcaatga taacttctaa accatcgact cctgtagtcg caagaccatt
     5641 ggacatggta cctagatagg aaagtcccgt tgtagcaact tttccgtttg accaatcagc
     5701 cttgacttga cgctggcgcg tgtgatcagt aaaggcacgg caacggccgt taagccaatc
     5761 gatgacattt ttataagcct cgatttgctg gtagtctcca ttagtcatga aacctgtcga
     5821 gtctttggta ccaacacctg agacatagag attggcaaag cctctcggaa ggaagtagtc
     5881 gtttagtgta tagctagagt tgatgtgagt tagcttttcc tcagcctctg ctacaagctc
     5941 agcttgacct tggggttgga cgagatttag ttgaggttcc tctagctcaa tcttgtgagg
     6001 aagcttaacc tcaagctcaa cctccatctt gtagagagcc ttgtcactag ccttgtcatt
     6061 ggttccctga tgataagggc tggctgtcat gatggcaggg atttttccat caaaacgagg
     6121 gcgaataatg ctaaccttta ctaggtctga tagccctttt tggtcagtat cgacacgaga
     6181 ctcaacgtaa acgacttcac gaatgacatc ctggttagaa aaagtagcca aactcttgcc
     6241 gttaaagtag tggtagtcat tatcctccgg aataagacca tcactaacaa gttggtcgat
     6301 aagagtattt ccttttttgg tgcgagtatt gagtaactga tagagatttt caatcaagtc
     6361 accatatata atgggaaatc cagtttcttt acgaaaaacg tcactatctt cgaagtcaac
     6421 caaataagaa aagcctaaaa gttgaaaagc aacagtataa aaaatatctg ctgtcagttc
     6481 atcttctgat tgaaaaaatg tcagcaggtc tgttttttta tcagctgcta ggatagaaag
     6541 tgggtagttg gtgtcttgat aagtgaaaaa gaaacgacgt aaaaaggttt caagtgagtc
     6601 tttgtgattg gctgtatttt gtaaatcaaa gccacatttt tttagctcag ataagacatt
     6661 ttcttttgga aaattgatat aactatattg attaaaacgc atagaacctc catatagaat
     6721 gacagttaag gttattatat caaaaaaaaa gcagaaaggg aattgttaac ttcaaaagga
     6781 aataatccaa taaaaatgaa taaagtacta aattcaatat agagaacaga gtaacaataa
     6841 gaataataga tagggtataa aagttctagg agatttatat tatatgcttt ctatttttat
     6901 atacaatata gtataaatat aaaaacgatg acaaaaatac aaatgaatag aaaataaatt
     6961 agtaagctga tgaaattttt ctcaagagaa gctatttata ggtgaaaatg gtataatata
     7021 gtgagaagga tagaggagaa gtgtaaattg atcgcacaac tagatacaaa aacagtctat
     7081 agttttatgg aaagcgtcat ttcgatcgaa aagtatgtga gagcagctaa agaatacggc
     7141 tacactcatt tggctatgat ggatattgac aatctttatg gcgctttcga ctttctagag
     7201 attacaaaaa aatacggcat tcatcctttg ctagggcttg aaatgacagt gtttgtagat
     7261 gatcaggaag tgaatttgcg ctttttagct ctatctagtg tgggctatca gcagttgatg
     7321 aagctttcga cagccaagat gcagggggtg aaaacttggt cagtcctgtc ccagtacctg
     7381 gaggatatcg cggtcattgt gccttatttt gatagagttg agtcgttaga actaggctgt
     7441 gattactata taggggttta tccagaaaca ctagcaagcg aatttcatca tcctatctta
     7501 cctctttatc gggtcaacgc ttttgaaagc agggatagag aagttcttca agttttaaca
     7561 gcgattaaag aaaatctacc gctcagagaa gttcccttgc gttcgagaca agatgtcttt
     7621 atatcagcaa gttctttaga gaaactattc caagagtgtt ttccgcaagc tttggacaat
     7681 ttagaaaagc ttatttcagg catttcttac gacttggata ctagtctgaa actgcctcgt
     7741 tttaatccag ctagaccagc agtagaggag ttgagagagc gtgctgaact ggggcttgtt
     7801 cagaaggggt tgactagtaa agaatatcaa gatagactag accaagaatt gtctgttatt
     7861 catgatatgg gctttgatga ttatttcttg gttgtttggg atttgttgcg ttttggacga
     7921 tcgaatggct attatatggg aatgggaagg ggttctgcag taggcagttt ggtttcttat
     7981 gccttagaca tcacggggat tgacccagta gagaaaaatc tgatttttga acgctttctt
     8041 aatcgtgaac gctataccat gcctgatatt gatattgata tcccagatat ttatcgtcca
     8101 gattttatca gatatgttgg taataaatat ggtagtaaac atgcggcaca aatcgttact
     8161 ttttcaacct ttggagccaa gcaagctctt cgagatgttt tgaaacgctt tggtgtgcca
     8221 gagtatgaat tatctgcaat tactaagaaa atcagttttc gtgacaatct taagtcggcc
     8281 tatgagggca atctccagtt tcgtcagcaa atcaatagta agttagaata ccaaaaagct
     8341 tttgagattg cttgcaagat agagggctat ccaaggcaaa cctctgtcca tgcggctggt
     8401 gttgtaatta gtgaccaaga tttaaccaac tacattcctc taaagtatgg tgatgaaatt
     8461 ccactgactc agtatgatgc tcatggagtt gaggctagcg gacttttgaa gatggacttt
     8521 ctgggactac gaaatttgac ctttgtccag aagatgcaag agttgcttgc tgaaacagaa
     8581 ggtattcatc tgaaaattga agaaatcgat ttagaagaca aagaaacgtt agctttattt
     8641 gcctctggta atacaaaagg tatctttcaa tttgagcaac caggtgccat tcgtctgctt
     8701 aagcgtgtgc aaccagtctg ttttgaagat gtcgtcgcga ctacttctct aaatcgaccg
     8761 ggtgctagtg actatatcaa taattttgtg gcaagaaagc atgggcagga agaagtgact
     8821 gttctggatc cagtactgga ggatattttg gctccaacct acggcataat gctctatcag
     8881 gagcaggtta tgcaggttgc ccagcgattt gccggattta gtcttgggaa agccgatatt
     8941 ttgcgtcggg ctatggggaa aaaggatgcc tctgccatgc atgagatgag ggcttccttt
     9001 attcaaggtt cattagaagc tggtcatact gtggaaaaag cagagcaggt ctttgatgtt
     9061 atggagaagt ttgcaggcta tggttttaac aggtcacacg cctatgccta ctcagcattg
     9121 gccttccagt tggcttattt taaaacacat tatccagcca tattttatca gattatgtta
     9181 aattcttcca acagtgatta cttaatagat gcacttgaag caggttttga agtagcctct
     9241 ctatccatca acaccattcc ctatcacgat aaaattgcca acaaggccat ctatctaggt
     9301 ttgaaatcca ttaaaggagt cagtaatgat ttagctctct ggattattga aaatagacct
     9361 tattctaaca ttgaagattt tatagctaaa ttacctgaga attatctgaa acttcctctg
     9421 ctagaacctt tggtaaaagt tggtcttttc gattcatttg aaaaaaatcg tcaaaaagta
     9481 tttaataact tagctaatct atttgaattt gtgaaagagt tgggaagttt gtttggagat
     9541 gctatttata gttggcagga atcggaagat tggacggaac aagaaaaatt ttatatggaa
     9601 caagagcttt tagggatagg tgtcagcaaa catccactac aagctattgc aagtaaggct
     9661 atttacccga ttaccccaat cggaaatttg tcagaaaata gctatgctat tatcttggtt
     9721 gaagttcaga aaataaaagt gattcgtacc aaaaagggtg aaaatatggc cttcttacag
     9781 gcagatgata gtaagaaaaa attggatgtc actctctttt cagacttata tcgtcaggtt
     9841 ggacaggaaa taaaagaggg agccttctac tatgtaaaag gaaaaataca attacgtgat
     9901 ggccgtctgc aaatgattgc acaagaaata agagaagcag ttgctgaacg cttttggatt
     9961 caggttaaaa atcatgactc ggatcaagag atgtcacgta ttttagacca gtataaaggc
    10021 ccaatcccag tcatcatccg gtatgaagag gaacagaaaa ccatcgtttc tccccatcat
    10081 tttgtagcta aatctgatga attagaggcg aaattgaatg gaatagttat gaaaacgatt
    10141 tatcgctaaa attacggaaa atagaagaat tttcaacgta aatgtggtat aatcagtaag
    10201 aatgttaaaa gaaaaaggag cataaccaat atgaaacgta ttgctgtttt gactagtggt
    10261 ggagacgccc ctggtatgaa cgctgccatc cgtgcagttg ttcgtcaagc aatttcagaa
    10321 ggaatggaag tgtttggtat ctatgacgga tatgctggta tggttgccgg tgaaattcat
    10381 cccctagatg cagcttcagt aggggacatc atttctcgtg gtggtacttt ccttcactca
    10441 gctcgttacc cagagttcgc tcaacttgaa gggcaactta aagggattga gcaattgaaa
    10501 aaacacggaa ttgaaggtgt agttgttatc ggtggtgacg gatcttacca cggcgctatg
    10561 cgtttgactg aacatggctt cccagctatt ggtcttccag gtacaatcga taacgatatc
    10621 gttggtactg actttacaat cggttttgac acagcggtta ctactgccat ggacgctatc
    10681 gataagattc gtgatacatc atcaagtcac cgtcgtactt ttgtaatcga agttatggga
    10741 cgtaacgctg gtgatatcgc tctttgggct ggtattgcaa ctggtgctga tgaaatcatc
    10801 atccctgaag caggcttcaa gatggaagat atcgtagcaa gcatcaaagc tggttatgaa
    10861 tgtggtaaaa aacacaatat catcgtctta gctgaaggtg tgatgtcagc ggctgaattt
    10921 ggtcaaaaac ttaaagaagc tggagataca agcgaccttc gtgtaacaga acttggacat
    10981 attcaacgtg gtggttctcc aactgcgcgt gaccgtgttt tggcgtcacg tatgggtgca
    11041 catgctgtta aacttcttaa agaaggtatc ggtggtgttg cggttggtat tcgtaacgaa
    11101 aaaatggttg aaaatccaat tcttggtact gcagaagaag gggcattgtt tagccttact
    11161 gcagaaggta agattgtggt taacaacccg tacaaagccg atattgagct atctagcttg
    11221 aataagagct tgtcataatt tacaatttag ttaataagac caaaaaggtc atatataaag
    11281 gagtcacaaa aatcatgaac aaacgtgtaa agatcgttgc aactttgggt cctgcggtag
    11341 aaatccgtgg tggtaaaaaa ttcggtgagg acggatactg gggtgaaaaa cttgatgttg
    11401 aagcttcagc taaaaacatt gctaaattga ttgaagctgg tgctaacaca ttccgattca
    11461 acttctcaca cggcgaccac caagaacaag gtgagcgtat ggcaactgtt aaacttgcgg
    11521 aaaaaattgc aggtaaaaaa gttggtttcc ttcttgatac aaaaggacct gaaatccgta
    11581 cagaattgtt cgaaggtgaa gctaaagaat attcatacaa aactggtgaa aaaattcgtg
    11641 ttgcaactaa acaaggaatc aaatcaactc gtgaagtgat tgcgttgaac gttgctggtg
    11701 ctcttgatat ctatgatgat gttgaagttg gtcgtcaagt tttggttgac gatggtaaac
    11761 ttggtcttcg tgtggttgct aaagatgatg caactcgtga atttgaagtt gaagttgaaa
    11821 acgatggtat catcgctaaa caaaaaggtg tgaacatccc taacactaaa attcctttcc
    11881 cagctcttgc tgaacgcgat aacgacgata tccgtttcgg tcttgaacaa ggtatcaact
    11941 tcatcgcaat ttcattcgta cgtactgcaa aagatgtgaa cgaagttcgt gcaatctgtg
    12001 aagaaactgg aaacggacat gttcaattgt tcgctaaaat cgaaaaccaa caaggtatcg
    12061 ataacttaga cgaaatcatc gaagcagctg atggtattat gattgctcgt ggtgatatgg
    12121 gtatcgaagt accgttcgaa atggttccag tttatcaaaa aatgattatc aagaaagtca
    12181 atgctgcagg taaagttgtt atcactgcaa caaacatgct tgaaacaatg actgaaaaac
    12241 cacgtgcaac tcgttcagaa gtatcagacg tattcaacgc tgttatcgac ggaactgacg
    12301 ctacaatgct gtcaggcgag tctgcaaacg gtaaataccc actcgagtca gtaactacaa
    12361 tggctacaat cgacaagaac gctcaagctc ttcttaatga atacggacgt cttgattcag
    12421 attcatttga gcgtaactct aagacagaag taatggcttc tgctgttaaa gatgctacta
    12481 actcaatgga tatcaaattg gttgtaactc ttactaagac aggtcatact gcacgtttga
    12541 tttctaaata ccgtccaaat gctgacatct tagcattgac atttgacgaa ttgacagaac
    12601 gtggcttgat gttgaactgg ggtgttatcc caatgttgac agatgctcca tcttcaactg
    12661 acgatatgtt cgaaatcgct gaacgtaaag cgatagaagc aggtctcgtt gagtcaggcg
    12721 atgatatcgt tatcgttgct ggtgtgccag taggagaagc tgttcgcaca aacacaatgc
    12781 gtatccgcac agtacgttaa gaaaaatata aaaacctatc atatccagct ttagagcttg
    12841 tgtgataggc tttttgtata gagggtaaga aataggcaaa actttcataa tggattgata
    12901 ctcttcgaaa atcaaattca aaccacgtca gcgtcgcctt accgtatata tgttactgac
    12961 ttcgtcagtt ctatctacaa cctcaaagca gtgctttgag caacctgcgg ctagcttcct
    13021 agtttgctct ttgattttca ttgagtatga aataagatat gcacaaattg attaggaaat
    13081 tcaaatgaat ttctacaaat gttttagcaa tcgtaatgta cttgtctaga ttcgatctga
    13141 tatattttcg atttaatgat atggtattta aaacctccaa agtagcttgc tccattcttt
    13201 tacttacgtg agtgtagatg ttatttactg ttttagcgtt tttgtgttcc actctaacca
    13261 ttatagcatt cttctcagct agtgtactaa ggagtgtgtg cctgaaaata tgggaactaa
    13321 ggggctggtt tatcggtttc tctagtttag tatttgcctt ttgcaaagtg atcttaaatg
    13381 cctttctcta aatttacata tcactattgt ttaacaaaat ctaatctatt ttaggtcact
    13441 tattcttttt ttgaaatgta gaatgaactt tttcaaagtt tttcgaatct tttaaaatct
    13501 gtttgtttta tatcgccatt ctcccccttt tttaattctc cctatatagc ctgacaactt
    13561 tcccgatggt acgaatatgg ttgctttcgt ctaggtggat gtcggagtat tcgggattga
    13621 gtttttttga ggtagccttg gcggagtttc ttgacatagt tagtgccgtc tacttggaag
    13681 atgccgatgg tattatagtc aatctgtggg gtattcttga taaataggta gtcgctgttt
    13741 cttatctttg gctccatgga cttgctgacg acataagcga ttgggtcgta gtcgtctggg
    13801 ataatggaaa ctccataact aaatcgtcgt cctgcatcaa gcggctacct gcagagataa
    13861 actacctaac acgagagtaa gtagtctgtc tgtagtcgtc cagtctgatg atttttacga
    13921 tacttcgttt ttctgatcat acagttgcct ctcggcatag gtcagaactt taccttgtct
    13981 gggtggtgcc cgttggtcgt agatagattg gatatcgcta ggagaatcct tttgaactgg
    14041 aggaaagagg gcatcaatca agctactgaa tactttaact aagtcaaata tagtattttt
    14101 cttagtagac ctaacccttt tttcataatt tccaatggtg tttttactta taccaatctt
    14161 agtacccaat tcttattgag tccaaccatt actagtctat attgttttat agttgattga
    14221 gtttggaata gtacgctgta gctgctaaaa catttctagg aattaatttg cctttcctaa
    14281 tagagttgtt catatcttat ttcaatctac tatatttttc gcctctaaca atcgcaatct
    14341 cttctttatc tacgaatgaa atcgctttct atttttgtaa gtaaagcatc acacgaaatc
    14401 cacgaaaatg aaaacctttg ttgtgttttc gtaaaaaatt tgttgacaga gcacgaaacg
    14461 cctactataa ttaaatcaag cttaaggaaa taacaaaaaa acaaatcgga gggaaagacg
    14521 atgaacatat taaatgagaa agtaatcaat atctgtaaag cagtagttaa agaaacttta
    14581 atccaagaca tttaggagaa agaaatgaag aaatcaagaa aattagccac attaggaatc
    14641 tgttcagctt tgtttttagg cttggctgct tgtcaacaac aacatgctac ttctgagggg
    14701 acgaatcaaa ggcaaagcag ttcagcgaaa gttccatgga aagcttcata caccaaccta
    14761 aacaaccagg taagtacaga agaggtcaaa tctctcttat cagctcactt ggatccaaat
    14821 agtgttgatg cattttttaa tctcgttaat gactataata ccattgtcgg ctcaactggc
    14881 ttatcaggag atttcacttc ctttactcac accgaatacg atgttgagaa aatcagtcat
    14941 ctctggaatc aaaagaaggg cgattttgtt gggaccaact gccgtatcaa tagttattgt
    15001 cttttgaaaa attcagtcac cattccaaag cttgaaaaga atgaccagtt gcttttccta
    15061 gataatgatg cgattgataa aggaaaggtc tttgattcac aagataagga agagtttgat
    15121 attctatttt cgagagttcc aactgaggca actacagatg tcaaggttca cgctgaaaag
    15181 atggaaacat tcttctcaca atttcaattc aatgaaaaag ctcgaatgct gtctgtagtc
    15241 ttgcacgaca atctggatgg cgagtatctg tttgtaggcc acgttggggt cttagtacct
    15301 gctgatgacg gtttcttatt tgtagagaaa ttgactttcg aagagcccta ccaagcgatt
    15361 aaatttgcta gtaaggaaga ttgctacaag tatttggaca ccaagtatgc ggattataca
    15421 ggcgagggac tggctaagcc ttttatcatg gataatgata agtgggttaa actttaatag
    15481 tgttatgata tgacagtaat cttaggattt ttatttctat ttttgatgag ttggtgggtt
    15541 atttcaatcg ttaatagcca gaaaccacgc aaaaatgaaa catttattgg ctatgtccaa
    15601 agatacgata ttgatgggaa tggctatcaa tcattaccgt gtcctcagaa ctgagaggag
    15661 ttcttgaaat cgtaaaacca ctttgaacaa gagttacttc aacccattgg ctccgacgga
    15721 ttaagttgct ttcgtgaata ccaaaatcag ccgcaatttc ttcataagtg cggtattctc
    15781 gcacatattg aaagtggcca taagaaggtc ttctaggctt aatttaggtt ttcgtccacc
    15841 ttttgcgtgt ttaagttgat aagctgtttt taatacagct aacatctctt caaaagtcgt
    15901 gcgctgaaca ccaacaagac gcttaaatcg tgcatcagtt agttgtttac ttgcttcata
    15961 attcatagaa ctattatacc atgttttgtt tcgcaggaaa tctaatgcta aaatcttatc
    16021 agtgagaaga tttcttaaag tttattcttt gtagtttcat ctgtattagt acataattta
    16081 acttagagaa aataggagga ctaatcgaat ggaattaaaa gattttacag aaaaagaaca
    16141 ggaaatgatt aagaaaaggc ttacaatgtc taatattagt gacaaagaaa ctactgagaa
    16201 gattctggcg ctagtaccac aagacttgat taagcgaatc ccgttttttg tcagaaaaca
    16261 tgctacaaca cgtacgatta aacgcatttc aattgaatac cctgaactct acgctgtagc
    16321 tcaaacaagt ggtgagattc cagaaaaaga acgcgaagaa ttgcgtcaga ttatcacgac
    16381 tatctttgaa caaaagatga ataagcatag tattaagtag agaatgaaaa aatattttat
    16441 tagcggtttg ggaagcaatg cctatcatag caaggatttt tttcaagaac tagattcgca
    16501 ggtctatttt ctaaatccat atgaaaagca tcttcgagat gaaacagaat tgaaatcatg
    16561 gtttaaaaat gagattgtag aggaagaatc tatctgtctg ataggccatt ctcttggagg
    16621 agatttagct cgttatttcg catcggaatt tgaagaagta aagaaactga ttcttttgga
    16681 tggtggctat ctagatttag ataagatttt acctttggat acagagttag aggaaactaa
    16741 aaattatatc aaatctcaaa ttgtttcgga cttagctctt cttatttcta aagagaagtc
    16801 cgaagcaaag cattggtcag aaaatatgga gaaagctgta agacagtcct atcactggaa
    16861 tgttgagtat aatagatatg agttggctat aaattatgaa aatatagaag cgatactccg
    16921 cctacggaga aaaatacaag cttttaagag agaagtggga gataccttgt ttattagtcc
    16981 tcgctatcct aatggagcta catggagaga ggaagcccta aaagaattgc cagactattt
    17041 taatactatt tttttagaaa actttggcca tgagctttat actcaagcac ctaaagaaat
    17101 cgctagtcta atgaatgagt ggctcgctta ttttctatga gctacttgta ttgctggaaa
    17161 tggtataaga tgaaaatcta caagtcacta attttcatat aacaaaaaaa cgaacaggct
    17221 ctttttctgt tattcagaag agaacttgtt cgtttttatc ttgatttttt taaaaagctc
    17281 tatatacata gggattttct ggtttatcga ctttggtaaa gctgtcgatt tccaaggttg
    17341 gaaggttttg tttgagttct ttatggtagt gattgaccag ttttcctttg gctgtaatcc
    17401 aggtgttgtt ttcatactgc cgggtattgc ccttggtcag caatccatag acaccagaat
    17461 ctgcgataca gtggataatg ccgaatcgga acagaaattg actattggca tgactggggt
    17521 cgttatagac aaagcctgta aactggattg tcttgccctc aaattcatct ggatagtcgt
    17581 agatagcctc catgacttcc atatagtttt cattagtgat ctgaatacta tcttgggata
    17641 agtatttatc cgccgccgtt cgcatttcct tttcataggc tgattttgaa aaataagaac
    17701 tagtatctgg tttcaaatat tggcttgtcg tcccttcact tgtctgaata gctagatccg
    17761 ttccttccga tagggggaaa tgataacctt tagcagaaac ggtctgagaa tccaagctaa
    17821 cagttgggaa agttaagccg atgacaagtg gaatagccag aagagaaata ctcgtcatct
    17881 tggccaatcg gctgttcaga tgactgtggg ttttgacttg cttcatccag atatacaatt
    17941 gaacaattgc caagataaag gaaagcacca tggagatata ggccagatag gaatagtgca
    18001 tgttgatgta ctggtttagt ttgcccgaca gatggaggta aatagtcagt tcaaaatagc
    18061 cagctaaaac taaaaatcga atcataggat gactccaatc aagagagaat aggctaaaac
    18121 gaccagagtc acaatcgtaa tgaagtgact aataaatcgt gctttcaagt aatttttcat
    18181 catgagaata tttttgatat ccagcattgg gccaatgacg agaaaggcca gaactggtgc
    18241 caaaccgaaa ctcgagagta gagaagcacc tataaaggca tccgcctcac tacagagcga
    18301 aagaagaaag gctaaaagca tcaagagcag gatagcaaaa agaggagtcg cactgataga
    18361 ggtcagaatc cgagtcggaa cgtagacctg tattatagaa gcaaagaggc agccaaatac
    18421 caaataacgc cccgtatcaa aaaattcatc aatggcctgc acaaagactt gaaaaacttt
    18481 ttttgcagaa ctcaagtaag aaaaatcatg ctcatgacaa gccagacgat tttctttctg
    18541 aatcggttct tgccagaaaa atcctagaaa aattcctagt attacagcca caagaatgga
    18601 acccagagct cgtaatagtg cgacatggaa ggagttgcca aaggcagaat aggtcgcaaa
    18661 aagaacaatg ggattgataa caggtgctgt cacaagaaaa ggaacggccg tgtaacttgg
    18721 aacctttttt tccagaaaac gattgatgat ggggacgatt ccacattcac aagaagggaa
    18781 aagtataccg acaaaggtcc caaaaaagat tctcccccaa cgatttcgag ggagaaaatg
    18841 ataaaccttg tcaggtgtga tataaacttc aatcagccct gagacaatgc ttcctatcag
    18901 aacgaaggga agggcttcaa tgataatgga gagaaaaatg gctccagttt gaagtacact
    18961 agatgggagt tgttgaaaag cagtcatcta gtttttcttt tctgctttga cttttttctc
    19021 tttgtcatca gggaaagtaa cttgtttcaa atcagggagt ttagcaaact cttcaaacat
    19081 cttgtccaag tcgtctcgtt ttgtaaattt tacagccata ggtgtacctc gattctaaat
    19141 tctacctcta ttatactatt attttgcgga aaagggagtt ttttaatttg ttttcgatag
    19201 ttgatactct tcgaaaatct cttcaaacca cgtcagcttc gccttaccgt agta
//
LOCUS       DBEROU010000046        16865 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.046, whole genome shotgun sequence.
ACCESSION   DBEROU010000046 DBEROU010000000
VERSION     DBEROU010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16865
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.046"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..900)
                     /gene="phtA"
                     /locus_tag="ACL8CL_001743"
     CDS             complement(<1..900)
                     /gene="phtA"
                     /locus_tag="ACL8CL_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HGS1330947.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLAPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDATHADNVRTKEEINRQKQEHS
                     QHREGGTSANDGAVAFARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQASQSNDIDSLLKQLYKLPLS"
     gene            complement(1154..2887)
                     /gene="ptsP"
                     /locus_tag="ACL8CL_001744"
     CDS             complement(1154..2887)
                     /gene="ptsP"
                     /locus_tag="ACL8CL_001744"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014294264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HGS1330948.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKEAGEAYAKQKAEWALLKDAKTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVAYNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(2893..3156)
                     /locus_tag="ACL8CL_001745"
     CDS             complement(2893..3156)
                     /locus_tag="ACL8CL_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HGS1330949.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
     gene            3507..3725
                     /gene="nrdH"
                     /locus_tag="ACL8CL_001746"
     CDS             3507..3725
                     /gene="nrdH"
                     /locus_tag="ACL8CL_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HGS1330950.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
     gene            3806..5965
                     /gene="nrdE"
                     /locus_tag="ACL8CL_001747"
     CDS             3806..5965
                     /gene="nrdE"
                     /locus_tag="ACL8CL_001747"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HGS1330951.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYRPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            6153..7115
                     /gene="nrdF"
                     /locus_tag="ACL8CL_001748"
     CDS             6153..7115
                     /gene="nrdF"
                     /locus_tag="ACL8CL_001748"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HGS1330952.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            7297..8058
                     /locus_tag="ACL8CL_001749"
     CDS             7297..8058
                     /locus_tag="ACL8CL_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HGS1330953.1"
                     /translation="MLKQEKLTKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     YTFYNISNLDTIVSDSKLSDSILFKLSKHIKVIKP"
     gene            complement(8169..8378)
                     /locus_tag="ACL8CL_001750"
     CDS             complement(8169..8378)
                     /locus_tag="ACL8CL_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HGS1330954.1"
                     /translation="MFKDFIQSIYEKVYIINFEKCSQIPCLTSEELKSLGKWYVSTGK
                     EWICHSDDDLEEFKNLFLNFINPEE"
     gene            complement(8421..9827)
                     /gene="lacG"
                     /locus_tag="ACL8CL_001751"
     CDS             complement(8421..9827)
                     /gene="lacG"
                     /locus_tag="ACL8CL_001751"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033920 - 6-phospho-beta-galactosidase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HGS1330955.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDHENPADVRAAELEDIIHNKFILDATYLGHYSEKTLAGVEAILAANGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVPRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            complement(10042..11733)
                     /locus_tag="ACL8CL_001752"
     CDS             complement(10042..11733)
                     /locus_tag="ACL8CL_001752"
                     /EC_number="2.7.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0019197 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase complex [Evidence IEA];
                     GO_function: GO:0015155 - lactose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactose-specific PTS transporter subunit EIIC"
                     /protein_id="HGS1330956.1"
                     /translation="MNKLIAFIEKGKPFFEKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIESGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARYFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FNLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQEQFD"
     gene            complement(11733..12050)
                     /locus_tag="ACL8CL_001753"
     CDS             complement(11733..12050)
                     /locus_tag="ACL8CL_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1330957.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVQ"
     gene            complement(12086..12922)
                     /locus_tag="ACL8CL_001754"
     CDS             complement(12086..12922)
                     /locus_tag="ACL8CL_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HGS1330958.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKKKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLTDYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            complement(13156..14136)
                     /gene="lacD"
                     /locus_tag="ACL8CL_001755"
     CDS             complement(13156..14136)
                     /gene="lacD"
                     /locus_tag="ACL8CL_001755"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HGS1330959.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            complement(14138..15067)
                     /locus_tag="ACL8CL_001756"
     CDS             complement(14138..15067)
                     /locus_tag="ACL8CL_001756"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009024 - tagatose-6-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HGS1330960.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLPVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLDELKEVLQEPLFAGIEWI
                     IVSLGANGTFAKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQVLYDQLIVKEV"
     gene            complement(15078..15593)
                     /gene="lacB"
                     /locus_tag="ACL8CL_001757"
     CDS             complement(15078..15593)
                     /gene="lacB"
                     /locus_tag="ACL8CL_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HGS1330961.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGDYHD"
     gene            complement(15624..16049)
                     /gene="lacA"
                     /locus_tag="ACL8CL_001758"
     CDS             complement(15624..16049)
                     /gene="lacA"
                     /locus_tag="ACL8CL_001758"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050044 - galactose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0019512 - lactose catabolic process via
                     tagatose-6-phosphate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HGS1330962.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            complement(16206..16703)
                     /locus_tag="ACL8CL_001759"
                     /pseudo
     CDS             complement(16206..16703)
                     /locus_tag="ACL8CL_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234238.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(16719..>16865)
                     /locus_tag="ACL8CL_001760"
     CDS             complement(16719..>16865)
                     /locus_tag="ACL8CL_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HGS1330963.1"
                     /translation="NHALSKERSKVENIFAKVKTFKMFSTTYRNHRKRFGLRMNLIAG
                     IIGF"
BASE COUNT         5045 a         3512 c         3049 g         5259 t
ORIGIN      
        1 actcaaaggc agtttgtaga gctgtttcaa gagactatca atgtcattac tttgacttgc
       61 ttgactgtta gtgttgctgt tgttgcttgt gttagtattt gtagttcctg gattgcttac
      121 agaaggtacc cagtttgttc ttgaagtgtt atcgctattt tgtcggcgat aggttcttga
      181 atttgacaga tttccccgac cagataggaa ggcttctgca gcagctaact cgctagctga
      241 taactcattc ttaggaatgt aatggtaatg gtcgccgtga ggaacgatat aagcatcacc
      301 cgtgtcctca atgatatcag aagcattaaa gatataacca tcatctgtgg tgtagcgtcc
      361 ctgtgaacgt gcaaaggcta ccgcaccatc gtttgctgaa gtccctcctt cacgatgctg
      421 actatgttct tgtttttgtc gattgatttc ttcttttgta cggacattat ccgcatgagt
      481 tgcatcctta aggtaaacat agtattttcc atctaccttg ataacatatc cacccttgac
      541 ctcattaaca atatcctcat cttttagctt atagtttgga tctttcatga gtaattcttc
      601 actgaaaatc gcatcataag gaaccttacc attgtaataa tgatagtggt cgccatgtga
      661 agtgacatag ccttggtctg ttatcttgat gacgatttgc tcagcattga ttccttcacg
      721 cttgctaacc tcatcaggag ccaaattctc cgttttttgc gtcgcttgtt ttccatctat
      781 ataggaaaca cgattatttt ccttaaccgt tctagcttga tacagtccca actcgtaaga
      841 acaaacactt aaaatcaaag ctgccgcaga accaacaagg tatttcttat taattttcat
      901 cgaaactctc tttctatttg atatttctag ttgcaattca tttctaaatt gcagaaactc
      961 ccctctatta cttaactggt taatagttta ctcctaaatc tccaacttgt caagaatttt
     1021 atgaaccatt taagtatttt ttgttctgac cactactttt tcaaggttgg actttctata
     1081 agaaagattt tcttgaaaat tctttttaaa atatcaaaaa aatccctgta actgagttgc
     1141 agggactttt cgattaatca aaattaacgt attctttttg aagttcaaga acttcttcca
     1201 ttgttgagca ttctgtaagg gcacggtttg cgtactcttc catcttagct gtgtcgagtt
     1261 tcttcatcaa gctgcgtgta cgaagtacag atgttgctga catagagaac tcatccaagc
     1321 ccattccgac aagaagtgga acagcttgtt ggtcaccagc catctcacca cacataccag
     1381 cccatttacc ttcagcgtga gctgctttga tcacattgtt aatcaagcgt aggattgatg
     1441 ggttgtatgg ttggtaaagg tatgaaactt gttcgttcat acggtctgct gccattgtat
     1501 attggatcaa gtcatttgta ccaattgaga agaagtcaac ttctttagca aattggtctg
     1561 caagcatagc cgctgcagga atctcgatca tgataccaac ttgaatgtta tacgcaactg
     1621 caacaccttc agcaagaagg tttgcttttt cttcatcaaa gactgctttc gctgcacgga
     1681 attctttcaa gagcgcaacc attgggaaca tgatacgcaa ttgaccgtga acagacgcac
     1741 gaagaagagc acggatttgt gtgcggaaca tagcatctcc agtctcagag atagagatac
     1801 gaagagcacg gaatccaagg aatgggttca tttcgtgagg catatcgaag taaggaagtt
     1861 ccttatctcc accgatatcc attgtacgaa cgacaacagg tttaccgttc attccttcaa
     1921 gaacagcctt gtatgcttca tactgctcat cttcagttgg gaagtcttga gaatccatgt
     1981 acaagaactc tgtacggtaa agtccaacag cttctgcacc gttgttgtta acaccttcaa
     2041 cgtcttttgg agtaccgata ttagcagcca actcgaagtg tttaccgtca gcagtcactg
     2101 ttttagcgtc tttcaagagc gcccattcag ctttttgttt tgcataggct tcaccagctt
     2161 ctttaaattc tgccgcttgt tcatcagttg ggttgataat aacctcacca gtgatcccgt
     2221 taacagcaag gatgtcaccg tctttaacga tttcagtgat gttatttgta cctaatacag
     2281 cagcaatttc aagtgtacgt gccatgatag ctgagtggct tgtacgtcca ccgatgttgg
     2341 ttacaaaagc ttttacaaag tttttgtcca attgagctgt atctgaagga gtcaagtcat
     2401 gcgcaatcac aatcacttct tcattgatag aagctgggtt tggcaatttt ttaccaagaa
     2461 ggtttgccaa tacacgtttt gtcacgtcgc ggatatccgc tgcgcgttct tgcatgtatg
     2521 ggttgtcttc catgccttca aagatagtga taaacatatc tgtaacttct ttcagacctg
     2581 cttctgcatt cactttcttc gcacggatag tttccttgat ttggctgatc atttctgggt
     2641 cagcaagaac cattaagtga gcatcaaaaa cttgagctgc ttcttcaccg agcgtaccta
     2701 ctgctttctc gcgaataaca gaaagctcgt cttgtgatgc ctgtagagcg gcatcaaggc
     2761 gagcttcttc tgcgtttgta tcttcgactg taatagtctc aaatgacaaa tccggctgaa
     2821 cgagtagata tgcttttgca actgcaacac cgtcagatgc tgcgattcct ttaagcattt
     2881 ctgtcatttc ccttatgcca atccttcttt ttccattgtt tctgagattg cagcgatagc
     2941 gtcatctgca tctgcacctt cagctgagat agttacgtca gcaccttgac caacaccaag
     3001 actcataaca cccataattg atttaaggtt aactgattta cctttgtact caagagtgat
     3061 atctgaagca aatttgctag cagtttgtac caacaatgtt gctggacgtg cgtgaatacc
     3121 tgtttctgcc actacgtgga aatctttaga agccatagtt tgactctcct tttgttcttt
     3181 ctttttttga gttatatgtg ataaccctta caatttagta ttataccatc ttctaggatt
     3241 tttttcaagc attttctgga ttttccttca atttcctttc agataacttg ctgaaaatct
     3301 tctatcctag ataacaaaac acaggatata gtttcttcaa aaacaattta taggataatc
     3361 attgctattt cacaatacaa acactatata ttgtgtttat atatgaaaac aacaaaagaa
     3421 caccactata tttagtttaa atcattgaca aaattttatt ttctgatata ctaagatagt
     3481 attaaatttt gaagaggagt tacacaatgg taaccgttta ttctaaaaac aattgtgtcc
     3541 aatgtaaaat gaccaagcgt ttcttggaca gtaataatgt ctcttatcgt gaaatcaatc
     3601 ttgatgagca acctgagtac gtcgatcaag ttaaagagct cggttttagc gcagctcctg
     3661 ttatccaaac accaactgaa gtcttttcag gtttccaacc aggaaaactg aaacaattag
     3721 cataatctta gtacatcatc cagaagaaac tgcttttagg gctaacttag aagcctttct
     3781 tttgtaatta gataagggaa attttatggg attaaaacat cttgaagacg tgacttactt
     3841 ccgtcttaat aacgaaatta accgtcctgt taatggacaa atcatgcttc ataaagataa
     3901 ggaagccttg gatgctttct ttaaagaaaa tgtagttcca aacactatgg tttttgattc
     3961 aatcaaagac aaaatcaatt acctcattga acacaactac atcgaaacag cctttataaa
     4021 gaaataccgt ccagagttct tggaagaatt ggctcaattt atcaaagacc aaaacttcca
     4081 attcaagtca ttcatggcag cttataaatt ttacaatcaa tatgccttaa agactaatga
     4141 cggtgaatac tatcttgaaa atatggaaga ccgtgtcttc ttcaacgccc tttatttcgc
     4201 tgatgggaat gaagctgttg caatcgatat tgccaatgaa atcatccacc aacgctacca
     4261 acctgctact ccttccttct tgaatgctgg acgtgctcgt cgtggggagt tggtatcttg
     4321 tttcctaatc caagtgacgg atgatatgaa ctctatcgga cgttctatca actcagctct
     4381 tcaactttca cgtattggtg gtggtgtggg aattaccctc agcaaccttc gtgaagctgg
     4441 tgcacctatc aaaggctatg aaggagctgc ttcaggtgtc gttcctgtca tgaagctttt
     4501 tgaagacagc ttctcttact ccaaccaatt ggggcaacgt caaggtgccg gtgttgtcta
     4561 cctcaacgtc tttcacccag atatcatcgc tttcctttca actaagaaag aaaacgctga
     4621 tgaaaaagta cgtgtcaaga ctctatcact tggtgttgta gtacctgata aattctacga
     4681 attggctcgt aaaaatgaag aaatgtacct cttcagccct tactctgtag aaaaagaata
     4741 cggtgtgcca ttcaattaca tcgacattac tgaaaaatac gatgaattgg tcgcaaatcc
     4801 aaatattcgc aagacaaaaa tcaaggcgcg ggatttggaa actgaaatct ctaaattgca
     4861 acaagagtct ggctatcctt atgtagtcaa catcgatacg gctaaccgtg caaatcctgt
     4921 tgatggaaag attatcatga gtaacttgtg ttctgagatt cttcaagttc aagaaccaag
     4981 cttgatcaac gatgctcaag aattccttca aatgggaaca gatgtttcat gtaacctggg
     5041 ttcaaccaac gtggttaaca tgatgacttc acctgatttt ggtcgttcta tccgtgctat
     5101 ggttcgtgcc cttactttcg ttacagatag ttcacatatc gtagctgtac caacgattga
     5161 ccatggaaat agccaagccc acacctttgg tctcggtgcc atgggactgc acagctacct
     5221 tgcccaacaa ctcattgaat atggttcacc tgagtctgtt gaatttacaa gcatctactt
     5281 tatgcttatg aactactgga ccttggtaga gtcaaacaat attgcgcgtg aacgtggtat
     5341 taccttccac aactttgaaa aatcagacta tgctaacgga agttactttg acaagtatgt
     5401 aactggtgaa tttgttccaa catcagaccg tgttaaagaa ctcttcaaaa atgtctttat
     5461 ccctggtgtt gctgattggg ctgaacttcg cgacaaggtt caagaagatg gtctttacca
     5521 ccaaaatcgc cttgctgtag cgccaaatgg ttctatcagc tatatcaacg acgtttctgc
     5581 ttctatccac ccaattacgc aacgtatcga agaacgccaa gagaagaaaa ttggtaaaat
     5641 ctactaccct gctgctggct tgtctacaga aaccattcct tactacactt ctgcctacga
     5701 tatggatatg cgtaaagtca tcgatgttta cgctgctgcg actgaacacg tggatcaagg
     5761 actttcactc accctcttca tgcgtagtga cattccaaaa ggcctttacg aatggaagag
     5821 ggaaaataaa caaacgacac gtgacttgtc catccttcgt aactatgcct ttaacaaggg
     5881 aatcaagtct atctactacg tccgtacctt tacagatgac ggtggagaag tcggcgccaa
     5941 ccaatgtgaa agctgtgtga tttagtttct atcatagaaa cactattttt ccctaagcag
     6001 cactgataaa atcaaatcta ctatgtcata acataattta tgtaacattt taaaagtcgg
     6061 gcttccgact ttttgtgcga tactcctata gaattatggt aaagtagaaa taattgtgag
     6121 ttcgcaatac taaacacaga aagagatttt aaatggaaac ttactacaaa gccattaact
     6181 ggaatgccat cgaagatgtc atcgacaaat caacttggga aaaactgacg gagcaatttt
     6241 ggctcgatac acgtattccc ttatcaaacg acttggatga ctggagaaag ctatctaaca
     6301 aagagaaaga cttggtcgga aaagtttttg gtggtttaac ccttcttgac actatgcaat
     6361 ctgaaactgg ggttcaagcc cttcgcgcag acatccgtac accacatgag gaagctgttt
     6421 tcaataacat ccaatttatg gaatctgtcc acgctaaatc ttactcatca atcttttcta
     6481 ccttgaatac taaggctgag attgaagaaa ttttcgaatg gaccaatacc aatccttacc
     6541 tacaaaagaa ggctgagatt gtcaacgaaa tctacctaaa cggcagccca cttgaaaaga
     6601 aagttgccag cgtcttcctc gaaaccttcc tcttctactc tggtttcttc actcccctct
     6661 actatctcgg taacaacaaa ctagccaacg ttgcagaaat cattaaattg attattcgtg
     6721 acgagtctgt tcacggaacc tacattggtt acaaattcca acttggtttc aatgaattgc
     6781 ctgaagaaga gcaagaaaaa ctcaaagaat ggatgtacga cctactctat actctttatg
     6841 aaaatgaaga aggctacaca gagagtcttt atgacggtgt tggttggact gaagaggtta
     6901 aaaccttcct tcgctacaat gccaataaag ctctcatgaa catgggacaa gatccacttt
     6961 tcccagattc agctgaagat gtcaacccaa tcgttatgaa cggtatttca acaggaactt
     7021 ccaaccatga cttcttctct caagtcggaa atggttacct tcttggcgaa gttgaagcca
     7081 tgcaagacga tgactataac tacggtttag actaattctc catcactctc aatcatcaaa
     7141 aagtaaacta gaaacatcaa agttataacc caacaaaaat atcgaaagtt ttgaaaacaa
     7201 acttcgatat ttttgttttt gtttcatttt gttgtttttg tatttgaatg atgagtttgt
     7261 ttatggtaag atagtagtag gaaacgaggt gataatatgc tcaaacaaga aaaattaacc
     7321 aaaattttag agatagtaaa tagtaaagga accataacag ttaaacagat tatggacgaa
     7381 atagccgttt cagatatgac tgcaaggcgc tatttacagg aattagctga taaagatttg
     7441 ctgattcgtg tgcatggtgg agctgaaaaa cttcgaacca actccctttt gactaatgag
     7501 cgatcaaata ttgaaaaaca agccctccaa acggcagaaa aacaagaaat agcccatttt
     7561 gcaggcagtc tagtagaaga aagagaaact attttcattg gaccaggaac aacattagag
     7621 ttttttgcgc gtgagttgcc tattgacaat atccgcgtcg taaccaacag tctacctgtt
     7681 tttctgattt taagcgaacg aaaattaaca gatttgattt tgataggtgg aaattatcgc
     7741 gatattacag gtgcttttgt tggcacattg accctacaaa atctctctaa tctccaattt
     7801 tctaaagctt tcgttagctg taatggtatt caaaacggag ctctagctac ttttagcgag
     7861 gaagagggag aggctcaacg catcgcttta aataattcta ataaaaaata tttactcgca
     7921 gatcatagta agttcaataa gtttgatttt tatacttttt ataatatatc aaatcttgat
     7981 actattgttt cagattctaa actaagtgat tcaatccttt ttaagctatc taaacacatt
     8041 aaagtcatca agccttaata aactgaaaga gcctgggtaa caactcaatt tcagaagaaa
     8101 aaaagtaaat cttcccacaa taaaacgcat agtatcaggt gtttcaacac ctgatactat
     8161 gcgtttttct attcttcagg attgataaaa tttaaaaata gatttttaaa ttcttccaga
     8221 tcatcatctg aatgacaaat ccattcttta ccagtcgaga cataccattt tccaagactt
     8281 ttcaattctt cactcgtcaa acacggtatt tgagagcatt tttcgaaatt tataatataa
     8341 actttttcat aaatagattg gataaaatct ttgaacatct ttttgactca ctaaaattct
     8401 atatctaatg actaattcta ctagtctata atctgagttt ccgctacttt cttgtaccag
     8461 tgagctgatt tcttaggata acgttcttga gtttcaaaat ctacgtagaa gagaccataa
     8521 cgtttctcat aaccgtttga ccatgagaag acatccatta atgaccaaat gaagtaacct
     8581 tttacattag ctccatctgc aatcgcatca gataaaacct ccaagtgttg cttcacgtaa
     8641 tcaatacgtc catcatcgta aacagtgtta tcaacgaact catctttata tccaagacca
     8701 ttttcagtga tgtagatttt cttatagtta ggataatctt tcttcacgcg catgatttgg
     8761 tcatacaaac cttgagggta gataatccaa tcccaatccg tgcgtggtac atagtcagga
     8821 gctacacgac gaccaatacc tttgatttgg tatttagagc ttcctttttc acctttacca
     8881 ttatggataa tttcagtttc tccatcaaag gcttccatcc agtcactcat atagtagttg
     8941 attcctagga aatcattcaa gtcttttgca gcttctaatg ctgtaaaatc ttcttcacga
     9001 agatccaagc taccaccatt ggcagccaag atagcctcta cccctgccag agtcttttca
     9061 gagtagtgac ccagataggt tgcatccaaa ataaatttgt tgtgaataat atcttccaac
     9121 tcagctgcac gaacatctgc tggattttca tgatctagag gatatttagt aggcagggcg
     9181 tgaacaacac caatttcccc tttatagcct ttctctttgt acaattttac cgcgcgtgca
     9241 tgagacacca tcatattgtg gtgtgattga aagactttgg caaggtcgta ctggatacct
     9301 ggagggaatt tcccaaccaa atattgacca tcaccgattg gtccaatttc attaaaggtt
     9361 gtccaatagt ttacttctgg aaattcttca aaacagaaag cagcgtagtc tacaaaatgt
     9421 tcgatatttt cacggtttaa gaagtctcca tttgagtgga gagcttctgg cgtgtcaaag
     9481 tgatgaagag ttacaaaagg ctcaacatga cgtttgtgac actctgcaaa taaattatga
     9541 taaaactcaa cacctttagc atttacttgg ccgtaaccag tcgggaaaat acgtgaccaa
     9601 gcaatagaaa ttcgaatacc attgacacca tactcttctg ctagcttgag gtcaactgga
     9661 tatcgattgt aaaaatcact agctggttcg gcagtgtacc agtagttatc ctcaagatat
     9721 ttatcccaag caactggtcc ttttccatca gtatgtgtag caccttctgc ttgataagca
     9781 gctgttgcgc caccgaaaat aaagtctttt ggaagtgttt ttgtcatttt tttcacctat
     9841 ttcttgattt aaataaagta aaagtgagag gagaggagtc agtgagccat gcttctccat
     9901 ctcacttctt gtaccaagtc actgaattgt gacatgagga ggtaaaaacg atatcttttt
     9961 accgacgaat taataaggcg attaggaaat tcgccatagc gatttccgta acgatgggag
    10021 actgtttcac agttcctagc cttaatcgaa ttgctcttgt acgaatgcta gggcaccttt
    10081 tccatcacga gttaatttga tgtattgagc gccttctgtc ttagcaagtt taataccgag
    10141 cttatctgtt tctgccttca tatcttcaaa gtttgaagca acttgagggg caaggataac
    10201 tagattaaac tctggtaaca tttcacggtg agcaccatag ccgcctgctg ctgctttcac
    10261 agggacattg tattctgctg ctgccttatt caaagcattt gcaaggagtc cacttgtacc
    10321 tccaccagca cagagaacga ggacatttgt ttcttcagtg atggtatttt gtgctgcatc
    10381 gacacccgct ttttcaagaa tagcatccgc ttttgcagtg ttgaagtttg cagcaacttt
    10441 ttctttcaat tcatcattag acttacctga acgttcttct tcaagaattt gttcatcata
    10501 gactttaagg aatgggtaat aaataactac gtcaaccaca attagaaggg cagcaagaat
    10561 gaatgatagc acttggaagt tagttccaag aactagacct agtggagctg gagttgtcca
    10621 tggtagatta gcagtgaatg agttcattcc aagagtttca ataaagaatt tgaaaatcca
    10681 tacgtttgca attggagcaa agataaatgg aatgaagaag attggattca aaacaagagg
    10741 tgcaccaaac aagattggtt catttacacc gaagaaggta ggaactactg aagcacgtcc
    10801 gattgcacgg ttacgtttcg attttgtcaa ccacatgaac ataaatggaa cgaccaatgt
    10861 cgcacctgta ccacccatgg taacgataaa catttgtgta ccagaagtaa gaatcttgtc
    10921 tgcatgcatc ccttgttgga gaaggttcaa gttaacttcg gcattggcat aggtaatagc
    10981 tgcgatagct ggttcaacga tagatggacc atgaatccca acaaaccaga agaaggcaaa
    11041 ggcaccaaag ataatggtaa taccaaggta tccgtctgct gctgagaaga gtggtgcgaa
    11101 gaatttaccg attgattctg ccacactaga accaacaaaa taacgtgcta ataagtcaag
    11161 agcataaaga gaaacaacag atagagtgaa tggaatcaca tctttaaaga cttgtgagat
    11221 atttggtgga acttcgtcag gcatacgaat agtgacgttg ttcttaacac aaaccttata
    11281 gatggctaca gtaacaaagg cagcaaggaa ggctgaaagc aaaccttttg tccccaagaa
    11341 tccagtagct agaccacttt cgataggatc agctgccaac atcaacaaac caacaattgc
    11401 tgccaacaat gttgacatat agttgatttg attggttttt tccatgctcc ggtttactga
    11461 gtcagtcaat gacttagctg ttgtaccagc taccaagaga gccagaatac ccatagaata
    11521 gctataaggt ttcatcagaa aggctacaac ttcatcagac catttaaagc cccatgagtt
    11581 tggtacaaag gcaatcaaga taaagatact tgagaagaga ataacaggca tacctgcaat
    11641 gaaaccatca cgaatagcac gaagatagat attacgagat agtttttcaa agaaaggctt
    11701 tcctttctcg ataaatgcaa ttagtttatt cattattgaa ctcctctctt gtagagttca
    11761 attaaatgat gcatcaaatc ttttaacaag atagttgtca ttaagtggtc ttggccatgc
    11821 atcatggtta cactataagc caagtcctca cctgaagctt ccttagtcaa tagacttgtt
    11881 tgcgcgtggt gagcctctgc aatacagcta ccagcttcct ctaccagagc gtccgctttt
    11941 tcaaaatcac cagcttcagc agccttcaag gcttccaata gttttgaacg agcatcgcca
    12001 gcataggcta cgatttcaaa acctaacaat gttacttctt ctctattcat gatagaattc
    12061 tccttatata gttttaaata aatttttatg acaataaacg ttggatatgt agaactagat
    12121 aaactcgttc acttttatac aaatcaagac ccgtatgttg cgtaatcaca tcatagatct
    12181 tggaaccaat ctcgaaggct tttggatagg attgtttaat atgatcttcc atatccagaa
    12241 gtgattggtt atcatctcta gatctgtcta aataatccaa gaaataattc aaatggatca
    12301 taaagcgatc atagaaatgg ttattctttt tagttcgttg aattgcatag tccgttaaaa
    12361 cttcttcaac attcctgaga atttctttcc tcttatcaat cgactccaca agttccactt
    12421 cattttcacc ttcagcatta atgaaatgat aagcaatccg aataatttcg tcctcaggaa
    12481 aatgatctgc tagcttctga cggtaaattt caaaagcttc atttgcgatt tgaaaagcga
    12541 caggatactt agcggaaata tctggcagat tactatcctt gtaccttcct tgagttagag
    12601 cttgataaga acagtaaata tgatctgtca aggttacata gagatactct tgaatcggat
    12661 aatgatattt ctttgatagc ttatcaatga tttcataggt cactgtgata aaatcaagcg
    12721 gaacatcttt gagaagagcc ataaagtttt ctctggactc ttcggtcttc atccgaaaga
    12781 ttttctcaac ctgattttca gcaatcaaat cccccttctt ctttccgaat gcaattccct
    12841 taccaattac aatcacctct tctcccttat catttctgac aagcgagaca ttgtgattca
    12901 ttggatttag aattcgatac atgtcaacct ccttttatat cttaaaggta tataagtaca
    12961 aaaaatgacc acaaatgaac tagaaaatca gctgatttct aattcacctg tgatcatgcc
    13021 taatattact tagtaacact caccttgtat taacttgtga tttaagtata gcacaagtaa
    13081 gttattttgt aaacctttac atacaacttt ttttaaattt ttttagattc atgttcctaa
    13141 actaggagga ctttcttaca cacgttcttt ccatgaagtc gctgttgttt gaagaacttt
    13201 attgagctca tcaatgtttt caaatccagt tgtgcgaagc cattcgcgag ctgctgcttc
    13261 accatctttg atgtaagctt caactgatcc tgcccatgta gcacggccac aaagaactcc
    13321 gttaaagttt gcacctgatt catgagcaaa tacaagagta tcttggaaga gtttagctga
    13381 tacaccagca ctcaagtaga tgtatggcaa gttcgttgct tcatcttgcg ctttgaagaa
    13441 ggctgctgct tcttcacgtg tataaactac ttcaccttca gcgaagcctt caacatattt
    13501 aatgttaaca ggaacttcaa ctttcaagac atcaatgtta aagcgtgggt ctgaaaagac
    13561 tttcatagcg ccgataactt tgtgtggttt tactttcgcg tattctacag aacctgcatc
    13621 cgcaattttt tcatcgtaag caaggatttc aaggaagaat gggatatctt cagccacaca
    13681 ctcagaaccg atgcgttcga tgtaggcttg tttttcttga ttgagttcgt ctgagctatc
    13741 tacatcatag taaagcaaga atttaactgc atctgcacct tcttctttaa tacgttttgc
    13801 agaccagaca tccaagcagt ctggcaagcg ttttgtgctt gttgtgtcat aacctgtttt
    13861 ttcataagca aggagaagac cagctttttc atcaagagct ttagttgctg gaagtccata
    13921 ctcagggtca agaagcatag atgaagcgta tttagtcaat tcatctgcta ccaagacttt
    13981 aagttcttcc atttgggcca cagttggttc ttctgtttgg tgttgagcca tgaggcgttt
    14041 caaagcacca cgttggtcaa atgcaagagc tgagatgata ccattttcat cagaaagttt
    14101 ttctaagcgt acacgttttt gttctgttaa agccatttta tacctctttt actattaatt
    14161 gatcatatag aacttgatag ttggccatgt tgacatgacc agtcattttt tcttgagcat
    14221 tgagcatacc aaggacattt gccttgatga gtaattctgc atccgattct ttgtgaagaa
    14281 gtcctgaaga aattcctgcc acagtagagt ctccagatcc aacaggattt accacctgaa
    14341 ttctaggaat atctaccttg tagaaagtgt caccatgttt ggcaaaagta ccgttggcac
    14401 caagtgaaac gataatccat tcaatccctg caaacaaagg ttcttgaagt acttctttta
    14461 attcatccaa atcctcagaa acttctcttc caagaagctg agacaattct tcattatttg
    14521 gtttgatgac tgttggttta tggggtgatt caagaacagc ctgaagtgct gcacctgagc
    14581 agtccaagac tacaggcttg ccagcttgat tagcaagttc taccaagctc gcatagtaat
    14641 caactggaag gccagctggc agactacctg agatggccac tacttcaact gactccaaga
    14701 gttttttgaa atgttccaaa aagtcttgac cttcctgttc caatacttca ggaccttttt
    14761 caagaacttc tgtttggttg tctccgtgga gaatagcgat acagttacga gtttctccct
    14821 gaattgagaa gaaatctttc tttacttgat tatcgatatg ttcaaccaaa aactcaccaa
    14881 gtttgccacc cactaaacca gtagcaagaa cagaatcgcc aaattctgaa agtactcggg
    14941 taacattgag tcccttacca ccagccgttt tggttacatc caccacacga ttgacagtat
    15001 caatcttcaa ctcatccaag ggataggaaa tatcaatgga tgggttcatt gtaactgtta
    15061 aaatcatagt ttacctctta gtcgtggtag tctccgcgat cccatttttc aaggaattca
    15121 gtgaagaagt ttgcgtctgt ttgttgagca ttgtgacttt caaggtgttc aattttcgca
    15181 atcaattttt tgttttcttc agatggtttg tattcagcat gaatgaaagc ttcgatgata
    15241 tcacacataa gcaattcacc agtaatctta ccaccaaaac cgataacgtt ggcgttcaat
    15301 tgttctttag cataaagggc tgttgtcata tcacgaacca aggcagaacg aacacctgga
    15361 actttattta cagcgttgtt gataccaaca ccagtaccac agatacatac cccgagatca
    15421 gcttgaccac tagttactgc ttcccctact tttttaccaa agattgggta gtgagtccgt
    15481 gtatggtcat atgtaccaaa gtcaatgact tcatatcctt ttgatttcaa aaattctgaa
    15541 accgccattt tttcatcagt tacgatgtgg tcacatccaa ttgcaattct catttttaac
    15601 ttacctttct tactataatc taattagccc attttgttca acatgtcaac ccggatttgg
    15661 tgacgaccac cgtcgtattt accattaaca aatcctttag cgatgttttt agccaattca
    15721 tcaccaacaa gttgtgcccc catagtgatc atacgtgagt tgttgtggcc acgagtcata
    15781 taagctgaac gttcgtcaga tacttctgca gcaaccattc ctttgatctt agttgcaacc
    15841 ataaatggac cagctccata agcatcaatc acgataccaa ggttttgttc ttctttgttt
    15901 acttctgcag caacagcgag agtcacatca acaaagtctt gaccttcagc tgtaacatcc
    15961 acaaggtgga agttttcttt ttctaaaaag tctttcacaa cttctttcaa tctcaaacct
    16021 gcagcatctg caccgataac aatagacata ttgtatactc ctttttattt tttctatgct
    16081 ggattccagc agtgtattgg ctgtctttaa aaaacagtca attagtcctc acaaacataa
    16141 tatactacta ttgtttgaag aagtcaacag tttgtgtttg tttttgttat ttatttttta
    16201 cttttttata aaacttctat ctcaaaccct aattcttcaa cctgattggc ttctaagaga
    16261 caaacattct tcttatcttc taaatgatct ccttcttcaa ggaatgttga caagccagac
    16321 catggttcaa aggtaatgaa aggtcccttg ttcaaagttg accagatgat gaggtttgga
    16381 aattctgcaa aatgcacttt caatccctta tcatgtttaa gagaacgaag ggcaattgtt
    16441 cgagattgca attcatccaa agtcactgca tctgtgctga aaagatcata actaagatct
    16501 atttcttttt gtccctctag ccatggactt ctatcttgga aatccaacat acctgtttct
    16561 gggaaaggac gtggaacaga gcaaatctct tctttctcaa actctagata ataatcttca
    16621 tagacttcat catcaagtag aagacaatta aatcctggat gtccaccaat aaagtaaggc
    16681 ctgaccttgc tagtttcttt attagacttc ctgcaaaact agaatccgat aataccagca
    16741 atcaaattca ttcgtaatcc gaagcgttta cgatgatttc gataggttgt tgaaaacatt
    16801 ttaaacgttt ttactttggc aaagatattc tcaaccttgc ttctctcctt agatagcgca
    16861 tgatt
//
LOCUS       DBEROU010000047        16150 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.047, whole genome shotgun sequence.
ACCESSION   DBEROU010000047 DBEROU010000000
VERSION     DBEROU010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 16150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16150
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.047"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(166..2271)
                     /locus_tag="ACL8CL_001761"
     CDS             complement(166..2271)
                     /locus_tag="ACL8CL_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HGS1330964.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLAAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQAIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSHLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMEDGVLVIREKA"
     gene            complement(2880..3860)
                     /gene="mraY"
                     /locus_tag="ACL8CL_001762"
     CDS             complement(2880..3860)
                     /gene="mraY"
                     /locus_tag="ACL8CL_001762"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HGS1330965.1"
                     /translation="MFISISAGIVTFLLTLVEIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            complement(3862..6114)
                     /gene="pbp2X"
                     /locus_tag="ACL8CL_001763"
                     /gene_synonym="pbpX"
     CDS             complement(3862..6114)
                     /gene="pbp2X"
                     /locus_tag="ACL8CL_001763"
                     /gene_synonym="pbpX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HGS1330966.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGLT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            complement(6118..6435)
                     /gene="ftsL"
                     /locus_tag="ACL8CL_001764"
     CDS             complement(6118..6435)
                     /gene="ftsL"
                     /locus_tag="ACL8CL_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HGS1330967.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            complement(6447..7397)
                     /gene="rsmH"
                     /locus_tag="ACL8CL_001765"
     CDS             complement(6447..7397)
                     /gene="rsmH"
                     /locus_tag="ACL8CL_001765"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HGS1330968.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKQIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            7563..7757
                     /locus_tag="ACL8CL_001766"
     CDS             7563..7757
                     /locus_tag="ACL8CL_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1330969.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            7773..8315
                     /locus_tag="ACL8CL_001767"
     CDS             7773..8315
                     /locus_tag="ACL8CL_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HGS1330970.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADRIGNQVFIVLFYLM
                     IFGNIIPLLLAYKYPQEVALIYPPLILVIALIASGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            8326..8568
                     /locus_tag="ACL8CL_001768"
     CDS             8326..8568
                     /locus_tag="ACL8CL_001768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330971.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            8579..8914
                     /locus_tag="ACL8CL_001769"
     CDS             8579..8914
                     /locus_tag="ACL8CL_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330972.1"
                     /translation="MKSLARLLIIHVFISIFLFFALTSGAISHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(9024..10025)
                     /locus_tag="ACL8CL_001770"
     CDS             complement(9024..10025)
                     /locus_tag="ACL8CL_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1330973.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(10085..11986)
                     /locus_tag="ACL8CL_001771"
     CDS             complement(10085..11986)
                     /locus_tag="ACL8CL_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
                     /protein_id="HGS1330974.1"
                     /translation="MKEERRQFFERVDGNQCRDYILSHCSKDYEKVKSSLERLMDNRF
                     MFDSPWDMESCSKIHQIQPMVWDQVFEDDPEWSYMLNRQEYLLQFMIGYLVEGDKDYI
                     QKCKFFLFDWIEQVREFSPQSLMTRTLDTGIRSFTWLKLLLLLLKFDLLEEKELEKIL
                     VSLEKQIDFMKSHYRAKYTLSNWGILQTIPMLAIYHFFADKMDLEEAYHFASEELKQQ
                     IETQILGDGSQFEQSILYHVEVYKALLDLCLLLPDLQDSYQELLEKMATYIQMMTGLD
                     GRTLAFGDSDSTETTEMLSLSAVVLNKEDLLNGLDVKVDLLSLLFLGREKVKRLQEFE
                     KRAWQPRSMIFEDSGHVCIKDEHRYLFFKNGPLGSAHSHSDENSFCLQYQGQPIFIDA
                     GRYSYREIYERYLLKSAWSHSTCIVDGKAPERITGSWEYEYYPHSLFCHHKEREGVHY
                     IEGAYWSAEPDLPYLHKRKILMLVEDVWLLVDDIRCQGQHEALTQFILDKDVTYQDGK
                     INQLRLWSEVDFDLEDTIISPKYNELERSSKLTKRQFFENQMLDYTIIAHESFEIIRH
                     SVYQTDDREVENALAFEVKNDETDKLILLLSKDICVGEKLCLVDGTKMRGKCLVYDKI
                     NERMIRLQC"
     gene            complement(12008..12301)
                     /gene="yajC"
                     /locus_tag="ACL8CL_001772"
     CDS             complement(12008..12301)
                     /gene="yajC"
                     /locus_tag="ACL8CL_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HGS1330975.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            complement(12301..13119)
                     /locus_tag="ACL8CL_001773"
     CDS             complement(12301..13119)
                     /locus_tag="ACL8CL_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HGS1330976.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPTKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            complement(13106..13885)
                     /locus_tag="ACL8CL_001774"
     CDS             complement(13106..13885)
                     /locus_tag="ACL8CL_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HGS1330977.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            complement(13900..14391)
                     /locus_tag="ACL8CL_001775"
     CDS             complement(13900..14391)
                     /locus_tag="ACL8CL_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1330978.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVPIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            complement(14402..15592)
                     /locus_tag="ACL8CL_001776"
     CDS             complement(14402..15592)
                     /locus_tag="ACL8CL_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HGS1330979.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGETREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            complement(15604..16038)
                     /locus_tag="ACL8CL_001777"
     CDS             complement(15604..16038)
                     /locus_tag="ACL8CL_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1330980.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
BASE COUNT         4695 a         3660 c         2854 g         4941 t
ORIGIN      
        1 tcaaggactg gttttgatat agcacgttta agtaccgact tctgagctac tatagtagat
       61 tgaaactaga ataatacact tctactatac gacatgcccg tcgtacctca aaaggaacca
      121 gtttttttca gctggctcct tcttttttat cctcaaaatt ctatcttagg ctttctcacg
      181 aataaccaaa acgccatctt ccatatctgc ttccagatgt ttagcatcta aatgatccaa
      241 gtggaagtct gtcaccttat cacgaatttc ttgttcaacc actcgacgga gaggacgaac
      301 tcccatgact tcgtcgtaac cttcttctgt gatataatct ttagccgctt gactgactac
      361 caagtcaatg tctttcttag ccaaggtttg gttaacttca gccaacatca aatctacaat
      421 cttagaaagg tcttccttag tcaagtgtga gaattcgatg actgcattaa agcggttgag
      481 gaattctgga cggaagaagg gtttcaaacg gtccatcaat tctggtttat ccgcatcttc
      541 tgtcaagttg gcttcatagc caaatccagc atttgaggtc gcaataatga cagtgttctt
      601 gaagtttact gtatttcctt gaccatctgt caaacgacca tcatctagaa cttggaggag
      661 aagggtaata acttgagggt cagccttttc aatttcatcc aagagaatga tagagtatgg
      721 attgcgacga acacgttctg ttaaggtatt gctattgtca tcataaccca catagcctgc
      781 tgttgtacca attagcttag aaacagctgt gcggtcactg tattcagaca tatccaaacg
      841 gataatcgca tcctgggttc caaacatatc gagtgccaat tgcttagcaa gctccgtctt
      901 accaacccca gtagacccta caaagaggaa gctgccgatt gggcgatttc cttcatcaaa
      961 accagcacgg ttacgacgga tagcacgagc tacagcttct acggccttat cttgaccaat
     1021 caccttgtct tgcaagcgat gagccatatc tttcaaacgt tcgatatctg aagctcccat
     1081 ttgcgatact gggatacctg tcattcgttc cacagattca gccacatcgt tgacacttgc
     1141 agtcactttc atatcttctg tgtggttttc gattttcttt tccaattctg caatgcgtgt
     1201 tttatagttt agagctgctt caaaatcttc tgcttcaact gctttttctt gcttgtcttt
     1261 ttccgtttcg atttctcgtt caacagcatg cacatctgtt actggatgtt gagccgccaa
     1321 gtgagcagcc gttacatcga caaggtcaat agccttatct ggcaagctac gttgaggaat
     1381 gtattgaaca gaataatcca ccgctgcttt caagacttcg tctggcaaga tgacattgtg
     1441 gtgttgttga tagaggtcac gaatggcttg aagaatttta aaagtattct ctgctgaagg
     1501 agcattgacc ttcacttcgt tgaaacgacg agcaagagca gcattcttca agatggtgtt
     1561 acggtattcg tcttgagttg ttgccccaat cactgtcaat tctccacgag agagagctgg
     1621 cttgagaata tccgcaagtc ctttagaacc actgtctcca ccagtgctac cagcaccaag
     1681 aatttggtga atttcatcaa agaagaggat aatattccct gcttctttca cttcattgac
     1741 taagttttgg acattttctt caaagctacc acggtattga gtaccagcct caagacctga
     1801 gatatcaata gaaataattt ccttgttctt gatagcagca ggaacatctc cgttcacaat
     1861 ggcttgcgct agaccttcga caactgctgt cttaccaaca cctgcatctc cgaccaaaac
     1921 aggattgttc ttggtgcgac gtgagaggat ttcagatgtt tcttgaattt ccttgtttcg
     1981 tccgataaca ggatccaact tgccctcacg cgcttctgct gccaagtttc gacctagttt
     2041 tgcaaggaca ccgtcttgtt tcatacctga tgcctgttgt ggcatttgca catcagtttc
     2101 tgcatttcct ggtaattgac cagtcgcacg atagtgagca aattcctcag gtgtgacttc
     2161 gcgtccatta attaagtaac ggcgattttc agaactatat cctcgcatac cacccatcaa
     2221 ttggttaaat aaatcatcca tgttattaaa attattaaag ttgttattca tattctttac
     2281 ctctttttgt ttacttagtt atgactactg atattgacta tctttgacca ttgttttaaa
     2341 aaatttagac tagctaaacc gctaccctac gtactatttc tggtttttct ttttcaagca
     2401 ggagtagact atctttactt ctgaaaattt ctagatgaaa catagaccca cctctttcta
     2461 ttttacttta aataagtgtt ctagtaaggc tttcatttac cttacgttca taatatacta
     2521 cattggtcag aaaaggtcaa gagttttgac tttaactgac caatattttt ttacactctt
     2581 cgaaaacctc ttcaaaccac atcagcttct ccttatctac aacctcaaaa ccatgttttg
     2641 agctgacttc gtcagttcta tcaaccatct caaaactata tattaaaatg aaataagaac
     2701 aggacaaatc gatcaggaca gtcaaatcga tttctaacaa tattttagaa gcagatgtgt
     2761 actattctag tttcaatcta ctatattttg agcagcctaa agttagcttt ctagtttgtt
     2821 ctttgatttt cattgtgtat ttaaatacaa aaacaccctg aaacatcagg gtgccattct
     2881 tacattaaat acaaaattgc tagggtcagg agacttgcta gaagtcccac tccccaaaag
     2941 aagaagtcaa ccttccactc gctccaagga tttcctttac cagacaatcc cccaagctca
     3001 aaatggtgat gtacaggcgt catacggaaa atacgtttac cacctgtcag tttgaaataa
     3061 ctgacttgca tcataacaga agttgtttca aaaacatata caattccgat aatcaagaga
     3121 gtccattctt ggtggagagc catagagata gctgccagca tcccacctag ggccaaactt
     3181 cccacatcac ccataaagac cttggcaggc ttatggttaa agatgaagaa accgagcaaa
     3241 ccaccaatca tggcaagaat cactagaaga atatccatct gaccttgcac ataggcaata
     3301 actccatagg cagacaaact aatcacaacg gaaatactag ctaaaccgtc aacaccgtct
     3361 gtcaagttta ctgcgtttga aaaaccgact agccagaaaa gagcgaagaa aatatagaaa
     3421 aatcccaaat gaactggata accaaagaca gacaggatat cgccaccgcg ctcatagaaa
     3481 agatagaaga taactccacc tagaagctga agagctaatt tttgcttagg attaagcccc
     3541 tcattgattt tacgaaagac cttgagaaag tcatctaaaa atccgaccaa gccatacaag
     3601 accaagatga acaaaatcat tcccacatta ttgctgaatt ggctactaaa tagggcgaaa
     3661 aagaaagcaa ccaaaacaga agtaatcaag aaaaccaaac ctcccattgt aggagtccca
     3721 gcttttgcct gatgctgttt gacatcctca tgcatctgct ggcctgtaat ttgcgccttt
     3781 ctataaaatt ggataaaggc cggaatttct actaaagtta gtaaaaatgt cacaattcca
     3841 gcactgatgg aaataaacat attagtctcc taaagttaat gtaatttttt taatgtcctt
     3901 gatagctgtg ttagcacgaa catcttgctt ctgcacagta gagcccgaac cttgaaattc
     3961 aagttctata ttgagccact tagcaagggt ctcagcagtc tcctttgtcc aaccatacat
     4021 atctggaacc tcctctgctt tatcagataa gataaggact tgctggttcg gggcaagatt
     4081 cttcccttct tcagcagaac tgtttttaat cttcgttcct gttcccacaa cgatgggttg
     4141 tacaagattg cgacgcaatt cttctgctaa atcaccaggt gaaatatcct tgacactagg
     4201 cataggataa ggactttgtt gacttacttg ctctaaagcc ttagctgttg tttgaagatt
     4261 gagagagtct ttcatagctg aagcccgctc caagatagga ttggcaaatt ctcccaactg
     4321 aatacctgaa taatgttcag gttgttggac cgtcacatac aagataaaat caggattttc
     4381 agccggactc atcgatacag ccgagaaaat atagtcggtt aacccgacta gataaccacc
     4441 atttttctcg tcagcaatct gagccgtacc agacttgagg gctacatttt gcccaggaac
     4501 agttacagtt ggcttgcctg tgctgtggtt atacatggtt ccataaaccg gatccgtccc
     4561 taccaaaacc atgttagtcc gagttagact agctgcatct ttagaaacag gatttcccac
     4621 aatttctttt tgagatttcc gagcagtttg atcatttgga tcataaatgg cactaataaa
     4681 tttaggctcc agcatgacac cgtcattagc aatagctgta aaggcacgaa tcatttgcgt
     4741 ctgggtcact gaaatccctt gtccaaatga gctttgcgca atgttgacaa tattatccgc
     4801 aggaagctga ccagcatact catccgtcaa accgaaacgg gtcggaactc caaatttaaa
     4861 acgattaaga taatcaagcc aggtagcatc tcccatcttt tgctcaagga gagtcatccc
     4921 aacgttactt gagtgtgcaa aaccttgaga aaaagtcatc attctgccac cagtcaatcc
     4981 ttcattaacg tcccaatctc gaatcgtggc atctgcaatt tttaactcac tactattaaa
     5041 gacttctcct cctggaaagg tattattatc aatagcagca gccaacatca tcactttcat
     5101 agtggaacct ggctcatagt tactttggta aaggatatca cgccaaacaa agtcctctgt
     5161 aatgccttct tttgtatctg catcaaaggt cggtcgttgc gttgttgcca gaatttcccc
     5221 tgttttagca ctgaccaaag tcgctgtcat gtactttcct tttaccttct cttgaaaagc
     5281 atccatctgg gtttccataa aggactggag ggggctggaa atggttgtat aaacatcctt
     5341 accgtccatc gttcgttggg aaacttgttc tgttccgggt acaatattac ccagacgatc
     5401 cttttcatag gtaataatgc cgtctgtccc tgcaagaata ctgttcaagg aactctccat
     5461 tccagaggtt cccagcaagc tcttgcttcc atcttcattt tcatggagct gagctaggcc
     5521 gataaaacta gaagcaaatt gtccgtttgg gtaactacga ttgggactgg ttgtaaaatc
     5581 aatccccttg acctctgcag cttccaattc ttttttgata gacatcatat tggcataggt
     5641 aatcccattt ccctttgctc caaaggaaac ttgcttgaga ttaggttgcg agagttgctc
     5701 tcttacatag gattcttcca tgtccagata cttatgaaag acctctgcaa ccttgttaaa
     5761 ttgtgttttt tctacgtaaa gaatcttacc cgttgctgac ttatagttct catcaatgac
     5821 cgcatagaca ttataggagg ttgcatcctc agcaatcggg actccatttc ggtcataaat
     5881 agtcccacgt ttggcaggaa ctgtacgggt ggtttgatga accttcttag cttccttcgc
     5941 taaatctgtt ccaaagcgag tgcctgtccc aataatgacc gcaaaattga ctaaaaaaat
     6001 ggcaaaaaca aagacagata ataaactcag actttttcca actctgcgtc tgttttcagc
     6061 cggcgatttc cgatttttgg tcgcataacg gattactctt tttgtccact tcatatctta
     6121 ctccgctatt ctaatatttt cattgtttaa ttgcaaatcg tgtgaattgg caatttcttt
     6181 caaacgttct gcacgtaata gttcattgac ctcttgcttg gcatcgtcca attcggtctt
     6241 cttttcctct atctgcgcat tgatttttgt caaatcattc tgcacttgca agagcttggt
     6301 ctgcataaaa ataatactaa tggctacaat aagagtggtt acagcaatgg aaaagtaaaa
     6361 agctttttcc acacgcgaaa accgtttaag ttgcatctgt agtatttgac ctgttttttc
     6421 cattttttct gccatctttt tccctcttac ttgtgaattt ttctggccac gcgcaacttg
     6481 gctgagtgcg agcggttatt ggcttctaac tcttccgcac ttggcaagat tggcttacgg
     6541 gacaccaatt ccatcttggg cttgagatca tctgggatga aaggcaagcc ttttggaact
     6601 tcaactgttg aagcttcctt gaacaattgc ttggtcaagc ggtcttctaa ggaatgaaag
     6661 gtaatcactg aaattctacc atccagagcc aacatatcca tagcctgctg gatggactca
     6721 tctgccgccc ccagttcatc attgacttca attcgaatag cctggaaaat ctgcttagca
     6781 ggatgcccct tcttcttgag ttccttggca ggtttgacca acttgataat ctctgctaac
     6841 tcagtcgttg tctcaatcgg cttcacttca cgcgcttgct caatcttacg cgcaatctgt
     6901 ttagagaatt tgtcctctcc atacttgaag aaaatacgaa ccaagtcatg atagtcataa
     6961 ttgttcacca cttcatacgc tgtcagacta gcatcctgat tcatccgcat gtccagtggc
     7021 gcatcctttt tataagaaaa accacgctca cgctggtcta attgaggact agacactccc
     7081 aagtcataac aaattccatc aatttcctga acaccagctt cgcgcaaacg tgcctgtaaa
     7141 tgacggaagt tgtccttgat aaaggtcacc attcccttct caatgtaagg tgccaagcgt
     7201 ttttgcgcat tgtcaatggc attctgatcc tggtcaaagg catagagatg gcctttttca
     7261 cttaatttac ttaataaata ctcgctatgg cctgctccac ccaaagtcgc atcaacgtag
     7321 ataccgtcag gttttacgtc aagcatatca atcgtttcgt ggagtaagac cgttacatga
     7381 tgaaattctt ttgtcatatc ttatctattt taccacaaat ccgaccagct tgcacttgtc
     7441 agacaaatag gacaaggaga ctactatttc ttcaaaaaat atgtcaaaaa tacttgacac
     7501 tttatgttta aaagactata atatagtcaa atatatacga cataaatcag aaaggagacc
     7561 agatgaatcg tgtgaaagaa tttcgcaagg aattgggtat ttcccagctc gaactcgcca
     7621 aggatatcgg tgtctcgaga cagactatca atatgattga aaacgacaag tacaatccaa
     7681 ccttggaact ctgtctcaat ctcgcccgca gcctccaaac tgacctcaac agtctctttt
     7741 gggaggaaga tttttaaaaa aggagcaaac tcatgaaaaa agaaaccttc actgaaaaac
     7801 tgatcaaacg cacatacggt atttctggtc cccttgacga atacaaacgg cgtgaggccg
     7861 atcgtattgg gaaccaagtc tttatcgtcc tcttttatct gatgattttc ggaaatatta
     7921 ttccactcct tctggcctat aaataccctc aagaagtggc tctaatctat cctcctctga
     7981 ttttagtgat tgccctcatc gcttctggct atgtcaccta ccaaatgaaa aaaacaggca
     8041 tcacagtcat tgaaccagat atgctgaatg agaaagaaag caagcaacta cactaccctg
     8101 gtctgaaagc aggtttgttc tttggtctat ggatgttttt tataactcct cttctcagta
     8161 tactcataga tgaaggtcag gactattttc attctcttct cactataaga aatggtgtat
     8221 caagtattct cggttctatt ttcttcggag cgagcataca gttcctcatc tcccgtcgca
     8281 ttgcaaaaac taagaaaaat caagatgagg attaggagga aactcatgaa aaaagaaaag
     8341 aaacagttac gttatcctgg tctgaaagca ggttcgattt atggaacagt gatatttttt
     8401 ataattccac tcattgatac cctaacaagt gaaaatccaa attttatcag ttctcttcta
     8461 aatacaaaac atatttttaa aactatactg ggagctttct ttttcggagt gatgatacat
     8521 attgtcgact cacttcgtat tgcaagagct aaaaaagacc aggattagga ggtgccttat
     8581 gaaatcacta gctagactac tgatcattca tgtttttatc agtattttcc ttttcttcgc
     8641 ccttacttca ggagctattt ctcatacagt tttactactc ctactcctct ttcttcctgc
     8701 gctcaataaa ggacttgaga aaatacaatc aaaacggata cctgtcctca acgcagccct
     8761 cttctttctc ctcatatcct ttccacaact tttaaccaac cctgtccaat ggaaattttc
     8821 aatattccta gtcgtaacca tcatttcaag tttggcctac ttctataact tttatcaagt
     8881 agttaaagaa gtagatcaaa aacagttgat ttaggagctt gctcccataa tttctcattt
     8941 tataggcaaa taatcccacc tagcttgttc taggtgggat tattttattt cttaacagag
     9001 attgcaagtc attttccttc attttagaaa gtcgactctt tccaattcac actacaatcc
     9061 aagacttgtt gcctttcttc ttgattgcga ccttcaatct ggtcaatcaa aatctttgta
     9121 gcctgttgtc cttcctcaaa ggagggctga accagcgtcg aaacacttgg agaagaaaag
     9181 caagtccact ccgtattgtc aaaaccaatc aacccaactt gtggcaagtt ataattcaac
     9241 tctttgataa cggtaaagac tagaggtagg gcccaacagt tagggataaa taccagagtt
     9301 ttttcatcgg gatcgatttc tttttgtaaa aattccttaa tttgttccaa attcgtatgc
     9361 ttatcttcaa tggttagact ggcgtggcgc atattagcat ctgttaaagc atccacaaaa
     9421 ccacttgccc gctcaatccg agtactcaaa cgactcgtat ccgctgtaat caagagaaaa
     9481 tgctcataac ctttttcgat acaggactgg gtcatgtcat aaacggcatc atagttattg
     9541 gttttaaccc agctagtccg gtgttcatag agctgactat caaaaaagac cattttcttc
     9601 tttttctcat cgatgatacg agaatatttt cggaaattag aggtcggctg aataataaag
     9661 ccgtctactc ccaagagaag catgctttca atataccggt cctcactctc ttggctgtaa
     9721 ttactatttc ctatcattac ctggtagcca ttctggctgg cgatatcctc aattccctta
     9781 acaatttggt ttgagaaact gttggtaata tcaccgatca aaacaccgat taattttgtt
     9841 cgtttggagt ttaagctacg cgcaacaatg ctcggtttgt aatttgtttc atgaataact
     9901 ttttcaatct tttcacgtgt ctcttgggac attttttcat atttcccgtt taggtaaaat
     9961 gacacggttg ttttcgaggt ctgagccatt tccgcaatgt cttttatggt cagtttcttc
    10021 tccaatctaa aacacctcct actaatactt attataacat attcactatt caaaatgcct
    10081 atttctagca ctgcaagcga atcattctct catttatttt atcatatact agacattttc
    10141 cacgcatttt tgttccgtca acgaggcaca atttttcacc tacacaaata tccttgctta
    10201 ataacagaat cagcttgtct gtttcgtcat ttttcacttc aaaagccaga gcattttcca
    10261 cttcacgatc atctgtctgg tagacagaat gacggatgat ttcaaaactc tcatgcgcaa
    10321 tgatggtata atccagcatc tgattctcaa agaattggcg cttggtgagt ttgctacttc
    10381 tttcaagctc attgtattta ggagaaatga tggtatcttc caaatcaaaa tcaacttcac
    10441 tccatagtct caactgattg attttcccat cttgataggt cacatccttg tcaaggataa
    10501 actgagtcaa cgcctcatgc tgaccttgac acctgatgtc atctaccaag agccagacat
    10561 cctctaccaa catgaggatt tttctcttgt gaagataagg caaatcaggt tctgctgacc
    10621 aataagcccc ttcaatataa tgcactccct ccctttcttt atggtgacaa aacagggagt
    10681 gaggatagta ttcatattcc caggatcccg tgattctttc cggagctttc ccatctacaa
    10741 tgcaggtcga atgactccaa gcactcttta agagataacg ttcatatatc tcccgataag
    10801 aataacgccc agcatctatg aaaataggtt ggccttgata ctgtaagcaa aaactattct
    10861 cgtcactatg gctatgggca cttcctagcg gaccattttt gaaaaataga taacgatgtt
    10921 catccttaat gcagacatgt ccagagtctt caaagatcat ggacctaggc tgccaagctc
    10981 tcttttcaaa ttcctgcagt cgcttgacct tttctcgccc caggaacaag aggctaagca
    11041 aatcgacttt aacatccaga ccgttaagaa ggtcttcctt gttcaaaacc acagcagaca
    11101 ggctcaacat ttctgtcgtt tctgtagaat cgctatcacc aaaagccaaa gttcgtccat
    11161 ctaagcctgt catcatttga atataggtcg ccatcttttc cagcaactct tggtaactat
    11221 cttgcaagtc tggaagcaag agacacaaat ccagcaaggc tttataaacc tctacatgat
    11281 agagaatcga ctgttcaaac tggcttccat ctcctaaaat ctgtgtctca atttgctgtt
    11341 tcaactcctc tgaagcaaaa tggtaagctt cttctaggtc catcttatct gcaaagaaat
    11401 gatagatagc aagcatcgga attgtttgta aaattcccca gttactaagg gtgtacttgg
    11461 cgcgatagtg gcttttcata aagtcaatct gcttttctag actgaccaaa attttctcta
    11521 gttctttctc ctctagcaag tcaaatttca agaggagcaa gagtagtttc aaccaagtaa
    11581 aggaacgaat acccgtatcc aaggttctag tcatcaagga ttgaggagaa aattctctca
    11641 cctgctcaat ccaatcaaat agaaagaact tgcacttttg aatatagtcc ttatctcctt
    11701 ctaccagata ccctatcata aactgcaaga gatattcttg tcgattgagc atataagacc
    11761 attctggatc atcttcaaat acttgatccc ataccatcgg ctggatttga tggatttttg
    11821 aacaagactc catatcccaa ggactatcaa acataaaacg attgtccatc aagcgttcaa
    11881 gggaactctt gactttctca tagtcttttg aacagtggga caagatataa tcacgacatt
    11941 ggtttccatc gactctttca aaaaattgtc ttctttcttc tttcattatc tattaccaga
    12001 aaaagaacta cttaaaaagc agttcttttg tctttcccat tacactttcc ttttctacat
    12061 agatgaccac accttttgca atctgcaagg agaccaagtc atctttgata gaaatgattt
    12121 ttccatgaat tccagacaat aacaacactt catcaccaga tgttaaagaa gctaaatact
    12181 cttgtcgttg ctccatctgt ttgcgaagca acttttgctg acgaatagaa tgaaagcttg
    12241 acagtaaaag gggactcact gccaagacaa tcactattcc ataaaacaat gttgtatcca
    12301 ttaagctata atcttaagcc agcttccgat aattccgatg ataactgtta aaataacgag
    12361 tttatatgtt gtccatttct tttctttgat caagtagtaa actaaaagtg taaatagggc
    12421 tggtagaaga gctggagcaa ccttatcaag cattccctga atacttacga tactttgttt
    12481 agcgtctgct ttaacttccc ctgcagcaaa ggtaatcggc accataatct taacagatgt
    12541 cgctgccaaa ccagcaatta cggttacacc gataatattg gcaatacgag aaatcgttgc
    12601 catctgctcg cttagtttat caatcacagt tgttcctagt ttgtatccat acagaccagt
    12661 tgacaattta atcgctgtta aaatcgtatt catcgcaagg aagaacaaga ttggaccgac
    12721 aaccaagcct tcttgagcaa acgaagctgc gatggttgag aacaatggag ctaaacagaa
    12781 ttgagaaaga gaatccccaa tacctgccaa tggtcccatc aaggccatct tgatgctacg
    12841 tgtttctttt gttggtcgac cattttccaa cattacaaga tgcaagctgg taataaaagg
    12901 caggaagtgt gggttggtat tatagaattc acagttttct tccaaggctt ggtagaaacc
    12961 ttcctgatcc tctccatagt gttttttcaa agcaggatac atcacattgg catatcccaa
    13021 cccttgatag ttactatagt taaatccatt ttgacaaaag aatgcccgca aagacgtttt
    13081 aagataatca cgttttgtta atttgttaga tccagtcatc gtgtgcttcc tcctctacca
    13141 catgatccgc tgtttttggc ttgttataaa attcaatcaa agcaaagata gtacctacaa
    13201 ttgcaatacc aattgttggg atgtttagat aagctgcaca aacatatccc aacaagacaa
    13261 agggaatcaa ctctttctta gccatcactg acaagatcat cgcaaaaccg atagctggga
    13321 gcattttacc agcaactgtc aaacctgtaa gtaataccgg tggaatgtag tctacgagtt
    13381 tcaacaaggt atccattgaa agggcaccca agcaacccaa ggtaaatcca ataaaggcaa
    13441 acaaccaaat tgttgcattt agagtgaact tgaatttctt caaattatgg tttttcaagt
    13501 gcttcatcgc cgtttcaggc gcaccagcac gtacagtgta ggcgaaagtt tgtaagaatt
    13561 gaatcgccac agcaatcgga gtagagaggg caagagccgc ttctggactg actttaccag
    13621 cactagtgat agccatcaag gtaccaaaga taccaggtcc gattgggttt ggtggaacag
    13681 tacctccagc accaacaccg aatcccatat aagccaattc accaatagct cccattgcaa
    13741 gagcagtagg cagatcacct agaataattc cgacaccaaa tgacagaaca atacatctat
    13801 tggtgtaaat tcctaacagc attccactaa aacagaaagc tgtccataat ccaattaaaa
    13861 tcgcttgaaa tacattaatc gacataacga tctcctcttt taaatatagt ccataatgtt
    13921 gacttgaaca gctccatcat ttcctgttgg agttgttttc gtattaaatg ttacttgatg
    13981 agtttggctt aattccttga gggctgcctt gtcctcttca cccaggaaga tggagcgtgt
    14041 cacttgctct ttaccagggg cattgtgaat gttcccaata ttgatttctt tgatagggac
    14101 accaccttct accaaggtta aagcgtcctt tacatccttt acaacgataa agatcgtttg
    14161 agcaggatta gccttgtgaa tgatatcaat caccttttgc aaagggaaga aacgcatggc
    14221 aactgagtct ggcacaactg ttttcatcag agtttgttgc atcttgtccg tgcttacttc
    14281 gtcattggca acaatgaccg tattacaacc taggtatttt acccaaagtt gtccttgtcc
    14341 atgaatcaac cgttcatcga tacgggtcat aataatattt ggcattgtca tatttctcct
    14401 cctaccaata taggttccag tctttgtaga aacggataag ggcttctagg taatagtagt
    14461 caccccagat attgccttca tccactcctt tacctgaatg ccatgagtac acaccgtgga
    14521 ggagacttgt cccaccaggg gtaaattgat catttgcata atgttcgatc aaggaacgaa
    14581 gcatggcatg catagcatgt ttataaatat ctttgtcagc atccacctct gggagatgtt
    14641 ttagcatttc atgaatccca cagacggcga tagctgttgc tgaagaatct cgtgattgat
    14701 cactaccatc attaaaaatc aaatcccaat aggacacatg atcttttggc agacgattca
    14761 agaaataatt ggtcacaccc ttaaacaagt caaagcagga ctcgtctttt aagtgacgat
    14821 aagtcaaagg aataccatag actccccatg attgaccacg tgcccagcat gaatcatcac
    14881 tatacccttg tctcgttaca cctttaaagg gttgacctgt ctcaggatca aaatagaagg
    14941 tgtggaagga cgaagcgtca tcacggatta cattattagc tgaagcatag aaatggcttt
    15001 ctgcaatatc gtagtatttt tgatcgcctg tttcttgata agcaaagaat aagagttgga
    15061 tattgagcaa gcagtcgata atcaaacggt aatgctcttt cttgcccaag tctccccaag
    15121 cttgaataaa accacctttt tcttgatagc gttcaatcaa cttatctgca gctttcaagg
    15181 ttgcttctct agtctctcca tctccattta tcttatattc agccatacaa gacggtgtgt
    15241 acaagaagcc gagatcatga tgatccaatt ctactctctt attgacacga tccaggaaag
    15301 aaagaacatt tttatgagcg atgtttttaa atgcatcctg ttgactgtat tcataagcca
    15361 accacagttc tcctgtccag aaaccattgg tccattccgt gttatccatg attggataga
    15421 cattatcaaa ggtagctggc gtcgggaaat cttccttgaa atagtcaagg ttgagttcta
    15481 actgccgaat aaccttatcg attgcctggc cgacttcttc tttcgtcaga agtggtactt
    15541 ctaagaagcg ctcaggcgac tttatttttt caatcgtaac cttttttatc attctttcac
    15601 cctttaaata cccgattcga aatcttcttc ctcttcctct gcatccgttg aaaacaattc
    15661 tttaccattt acgactccgc cctgggccgc cactactgat ttattaacaa cctcatcaaa
    15721 gctatgagcc attctagcaa agactgcttc cattaacatg gctaagttca aacccgagag
    15781 aacattcatt gttttggctg gattttctcc cattatggta gaagaaacct tgaatggcga
    15841 tcctcccaag agatcactta ggattaaaac ttcttcttca tttgaaattg caagtaagat
    15901 tttttgcttg agttcatctg ctgacattcc ttccacaaag tcaatcgcct ccacattttc
    15961 ttgattgcct gcaatcaatt gtaaagaact ataaatccct gtagcaaaat gtccatgccc
    16021 tacaagtaca attttcatct tttctccttt cttgtttagg tttaccggtt tactttatat
    16081 tccccattgt aatcctttat ttttatcttg tcaatagttt ttttaaaaaa atattagcgc
    16141 tttcattatc
//
LOCUS       DBEROU010000048        14932 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.048, whole genome shotgun sequence.
ACCESSION   DBEROU010000048 DBEROU010000000
VERSION     DBEROU010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 14932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.048"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..200
                     /locus_tag="ACL8CL_001778"
     CDS             <1..200
                     /locus_tag="ACL8CL_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS1330981.1"
                     /translation="PEQKRRLSFKPGITGLWQVSGRSKITDFDDVVKLDVAYIDNWTI
                     WKDIEILLKTVKVVFMRDGAK"
     gene            231..1403
                     /gene="cps2T"
                     /locus_tag="ACL8CL_001779"
                     /gene_synonym="wchF"
     CDS             231..1403
                     /gene="cps2T"
                     /locus_tag="ACL8CL_001779"
                     /gene_synonym="wchF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta 1-4 rhamnosyltransferase Cps2T"
                     /protein_id="HGS1330982.1"
                     /translation="MKKSVYIIGSKGIPAKYGGFETFVEKLTAFQQDKTIQYYVACMR
                     ENSAKSGTTEDVFEHNGAICYNVDVPNIGPARAIVYDIAAINRAIKIAKENKDEDPIF
                     YILACRIGPFIHGIKKKIQEIGGTLLVNPDGHEWLRAKWSAPVRRYWKISEGLMVKHA
                     DLLVCDSKNIEKYIQEDYIQYQPKTTYIAYGTDTTRSTLNSSDEKVRAWFKEKNVSEN
                     EYYLVVGRFVPENNYESMIRGFLASSSKKDFVLITNVEQNKFYNQLLASTGFDKDPRV
                     KFVGTVYDQELLKYIRENAFAYFHGHEVGGTNPSLLEALASTKLNLLLDVGFNREVGE
                     QSAIYWKKDELSQVIEKVEQFDAKIIDELDRQSNQRIVDFFTWEKIVTDYEKLFKK"
     gene            1400..2671
                     /locus_tag="ACL8CL_001780"
     CDS             1400..2671
                     /locus_tag="ACL8CL_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS1330983.1"
                     /translation="MKILHYTLGFQPQRTGGLVKYAEDLMLEQIAQGYQVAALYPGRI
                     KFFSKKIEIIKETSRQFECYELLNSLPMALFGGISDPTAFLTPCDKNVYRTFLEKVQP
                     DIIHIHSFMGLHKEFLEIAKSLNIRVVFTSHDYYGLAPVPHFYFNGVDYSDKSTNLTW
                     NIMSSNALSVKKLRLFQVSFYPTIRKLLKLLGKNPKSKKNLVIRDVIEEQDYSELRYY
                     YNEMFHLIDGYLFNSRLAKKVYEINEIKSANSIVLSITNSSINHHQRLTTTNNKIRVA
                     YIGPDEEYKGYFDFVDFVETLDRESYEVATYGHLPNEECPSFIEQKGYFTKEMIDSVY
                     ENIDILIVPSKWKETFGLITVEALSYGVNVFVSENVGSKDLLPESHVFKNQNDLIVKF
                     LKNDIENTKLKTLDEHSIEVIQYYERVINDS"
     gene            2661..3347
                     /locus_tag="ACL8CL_001781"
     CDS             2661..3347
                     /locus_tag="ACL8CL_001781"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HGS1330984.1"
                     /translation="MIVKSFIKKITGKTVDIHPDVPLSYILQRGINYSFGLFRGVVRG
                     IGFGQNDKRLFIGQGVSILAKRKLFVGKNVRIGKKVSIDALSKEGIHFADNVKIGDYS
                     QIIGTGSIKNMGIGLKIGKNSSFSEYSLFGSAGGITIGDNVIAGQNVRFHAENHNYSD
                     LDKLIVEQGVSRKGISVGDNCWIGAGSVFLDGSSIGSGCVVAANSVITKNFPDNVIVA
                     GVPAKIIKMK"
     gene            3359..4264
                     /locus_tag="ACL8CL_001782"
     CDS             3359..4264
                     /locus_tag="ACL8CL_001782"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HGS1330985.1"
                     /translation="MRKEIKAVYAILNYNTWEDTARLAQKVATFQHIQSVIIVDNLST
                     DDSYHYLKRLEGEKISVYQTQRNGGYSVGNNFAARKAYNMGVDILFISNPDVDIDEKD
                     SLMIAQNLYKNSSYALLSGIEYNAMKEIDLPIVWHENSYYDDLLDCLFLTRKWRRKKE
                     DINLSMIKSSIIDVDLVKGSFFAVRLSDFHDVGYFDESVFLFCEERILAKKLQKVNKK
                     IGILPEAKYYHNHSTSINERYKKKKEQINLLYKSRYYYNVKYNNISFVKKLCLKSVMM
                     LSILEYIILDMMNLLYTKTNYKKIQ"
     gene            complement(4365..4727)
                     /locus_tag="ACL8CL_001783"
                     /pseudo
     CDS             complement(4365..4727)
                     /locus_tag="ACL8CL_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001857010.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4954..6216
                     /locus_tag="ACL8CL_001784"
     CDS             4954..6216
                     /locus_tag="ACL8CL_001784"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1330986.1"
                     /translation="MSKLILNLKLNSFLFVIILLLVVVTPTHTLIFDALIPTKIDNLW
                     REFLLVISFLCILKINAGKIKIGKLGGPIIVMGGIGLVYTICSDRPFTALNLFRIYMF
                     PAVIYFIIINCNFRKERLLILKQAHVYTACILALWGIFQAWVLKDQFLIKIGYPSQGN
                     FLKSTAFYIGGFFGQQRVTSTFSAPNLAGVYFGISLIILLSIFDTIKSNRLVLFSIVA
                     AFVLTFSRSAIISTLVGIVFFQRKKLFSTTKINVMTLVIFPLIFLIVLVIFYLYPENV
                     IINMLYSSYSSTLNLTDSSAVKHLEDLWLPLLKVIDYPLGLGFGNNGPIVLSLYHSAN
                     LVESSIYLLAYDFGILGMFIYLFPYFYTIFVYKKYLLSGAICCLVLITYLFLPNVENF
                     EIIFFIYLFIGMDELALYTKGMNVHEID"
     gene            6203..7363
                     /locus_tag="ACL8CL_001785"
     CDS             6203..7363
                     /locus_tag="ACL8CL_001785"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HGS1330987.1"
                     /translation="MKLIKLLKKYINLYISSSVPKTNKLLIFGSWLGEKYADNPRYLF
                     EYVIKNRPDLKAIWITSNQDVFKELQKKQYLVMMAEAPETRKIVKKAKYIFTATGIFD
                     IGEQNANFVGGAYLINLWHGIPLKKIMYDDKHSALHKRSKLVTWVEKIPLRNYFVIST
                     STAITQIYQSAFRVKKSNILELGQPRNDYFYDKSNPVSSLIQELKSKNIILYMPTHRN
                     EGKKQIDLDKLMDLEHLNNWCEETKSIFVIKKHFYHSKEKTIDKKYSSIIDVTNEKVD
                     VQELLKCSNVLITDYSSCYIDYLLLNRPIIFFNYDYDDYLRVDRSLYFPYENVTPGEK
                     CQNFDELLVTLQNLYVGKDDYREERENIKTFFYSSETQKSVSEKIINHVLNL"
     gene            7381..8820
                     /locus_tag="ACL8CL_001786"
     CDS             7381..8820
                     /locus_tag="ACL8CL_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HGS1330988.1"
                     /translation="MKKILNKYYSLSNPVKASIWFTICNVLQKGISMITVPVFTRVLT
                     TEQYGVYSVYQSWYSIIGVFATLNLYYGVFNNGMIKYEKDKNVFTSSMQGLTTTVTAI
                     FLLIYLIGIDFWNSLLGLPTLLILVMFFDLFFTPAYSFWVARQRFEYKYRNLVFITFI
                     IAIGSPIIGISAVVLSTYKAEARVISFVLVQSCIGLYFYILNLYRGKHFFCKKYWLYA
                     LNFNLPLIPHYLSQSVLNQSDRIMINSMVGMGEAAIYSVAYSISILMLLVTSAINSSF
                     IPYTYKCIRDKKYTELGKSANLLITLVGIGSILTISLGPEIIQLFAPKQYYEAIWIIP
                     PVALSVYFMFLYPIFGNIEFYFEANHFVMWASIGGAIMNIFLNFIFLKHFGYIAAGYT
                     TLFCYILFALGHYIFMRRVLYQNLSGIQIYNSRYLLFFSILLIILMLLIVIIYPFILI
                     RYLTISLILGGCFLKRKKIMSSIAIIKQQ"
     gene            8830..9645
                     /locus_tag="ACL8CL_001787"
     CDS             8830..9645
                     /locus_tag="ACL8CL_001787"
                     /inference="COORDINATES: protein motif:HMM:NF016852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HGS1330989.1"
                     /translation="MTETNKQLHETLIEILDFVKEICEKHELTYFLVFGTALGAKRHC
                     GFIPWDDDVDIALPREHYNIFIDALSETDQSIFSLQNEDNEPNYFLPFAKLRKNNTIF
                     IEKILDVEYENNGIYIDIFPLDFVENPDSFNFKIRRTTFNYIKHILKFSSCRSFYKNK
                     YSNVRYLIENIMSIPTLFFSNRRLLFLANSLISSTTKADFIGQYDQKSNKRAIMPSNY
                     YFPPRSAVFEGKTYSVPAKLEDYLKCFYGSDYMELPPIEKRVTHQPITLRFEK"
     gene            9673..10065
                     /gene="tagD"
                     /locus_tag="ACL8CL_001788"
     CDS             9673..10065
                     /gene="tagD"
                     /locus_tag="ACL8CL_001788"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047348 - glycerol-3-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019350 - teichoic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HGS1330990.1"
                     /translation="MKRVITYGTFDLLHHGHINLLRRAKELGDYLVVVVSSDEFNLIE
                     KNKVCYFNYEHRKSLVEAIRYVDLVIPETSWEQKRSDVKEYHIDTFVMGDDWIGEFDY
                     LKEEGVEVVYLPRTKEISTTKIKKDLSM"
     gene            10222..11091
                     /gene="rfbA"
                     /locus_tag="ACL8CL_001789"
     CDS             10222..11091
                     /gene="rfbA"
                     /locus_tag="ACL8CL_001789"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000676139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HGS1330991.1"
                     /translation="MKGIILAGGSGTRLYPLTRAASKQLMPVYDKPMIYYPLSTLMLA
                     GIRDILIISTPQDLHRFQELLQDGSEFGLKLSYAEQPSPDGLAQAFIIGEEFISDDSV
                     ALILGDNIYHGSGLSKMLQKAASKESGATVFGYHVKDPERFGVVEFDQDMKAISIEEK
                     PEQPRSNYAVTGLYFYDNDVVEIAKSIKPSPRGELEITDVNKAYLDRGDLSVELMGRG
                     FAWLDTGTHESLLEASQYIETVQRMQNVQVANLEEIAYRMGYISREDVLALAQPLKKN
                     EYGQYLLRLIGEA"
     gene            11092..11688
                     /locus_tag="ACL8CL_001790"
     CDS             11092..11688
                     /locus_tag="ACL8CL_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HGS1330992.1"
                     /translation="MTDNFFGKTLAARKVEAIPGMLEFDIPVHGDNRGWFKENFQKEK
                     MLPLGFPESFFAEGKLQNNVSFSRKNVLRGLHAEPWDKYISVADGGKVLGSWVDLREG
                     ETFGNTYQTVIDASKGIFVPRGVANGFQVLSDTVSYSYLVNDYWALELKPKYAFVNYA
                     DPSLGIEWENIAEAEVSEADKHHPLLKDVKPLKKEDLE"
     gene            11698..12747
                     /gene="rfbB"
                     /locus_tag="ACL8CL_001791"
     CDS             11698..12747
                     /gene="rfbB"
                     /locus_tag="ACL8CL_001791"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HGS1330993.1"
                     /translation="MTEYKNIIVTGGAGFIGSNFVHYVYENFPDVHVTVLDKLTYAGN
                     RANIEEILGNRVELVVGDIADAELVDKLAAQADAIVHYAAESHNDNSLNDPSPFIHTN
                     FIGTYTLLEAARKYDIRFHHVSTDEVYGDLPLREDLPGHGEGPGEKFTAETKYNPSSP
                     YSSTKAASDLIVKAWVRSFGVKATISNCSNNYGPYQHIEKFIPRQITNILSGIKPKLY
                     GEGKNVRDWIHTNDHSSGVWTILTKGQIGETYLIGADGEKNNKEVLELILKEMGQATD
                     AYDHVTDRAGHDLRYAIDASKLRDELGWKPEFTNFEAGLKATIKWYTDNQEWWKAEKE
                     AVEANYAKTQEIITV"
     gene            12813..13664
                     /gene="rfbD"
                     /locus_tag="ACL8CL_001792"
     CDS             12813..13664
                     /gene="rfbD"
                     /locus_tag="ACL8CL_001792"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HGS1330994.1"
                     /translation="MILITGANGQLGTELRYLLDERNEEYVAVDVAEMDITNEEMVEK
                     VFEEVKPTLVYHCAAYTAVDAAEDEGKELDFAINVTGTKNVAKASEKHGATLVYISTD
                     YVFDGKKPVGQEWEVDDRPDPQTEYGRTKRMGEELVEKHVSNFYIIRTAWVFGNYGKN
                     FVFTMQNLAKTHKTLTVVNDQYGRPTWTRTLAEFMTYLAENRKEFGYYHLSNDATEDT
                     TWYDFAVEILKDTDVEVKPVDSSQFPAKAKRPLNSTMSLAKAKATGFVIPTWQDALQE
                     FYKQEVR"
     gene            13761..14925
                     /locus_tag="ACL8CL_001793"
                     /pseudo
     CDS             13761..14925
                     /locus_tag="ACL8CL_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251597.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4930 a         2209 c         2933 g         4860 t
ORIGIN      
        1 ctccagaaca gaaacgtcga ctcagcttta aacccggtat tacaggttta tggcaggtta
       61 gcggccgtag taaaataacc gattttgacg atgttgtaaa attagatgtg gcttatattg
      121 ataattggac aatctggaaa gatattgaaa ttttgcttaa aactgttaaa gttgtattta
      181 tgagagatgg agcgaagtaa tttctgtata tccatcatat taggagagaa atgaaaaagt
      241 cagtttatat cattggttca aaaggaattc ctgctaagta tggaggattt gaaacttttg
      301 ttgaaaaatt aacagccttc caacaagata agactatcca atattatgtg gcttgtatgc
      361 gtgaaaactc tgcaaaatca gggactactg aggatgtttt tgaacataat ggtgctatct
      421 gttataacgt cgatgttcct aatattggtc cagcgcgagc catagtatat gatatcgcgg
      481 caattaacag agctattaaa attgccaaag aaaataagga tgaagatcca atcttctata
      541 ttctagcttg tcgaatcggt ccgttcatcc atggaattaa gaaaaaaatc caggagatag
      601 gtgggactct tctagttaat ccagatggtc acgagtggtt acgagctaag tggagcgctc
      661 cagttcgtcg ttattggaaa atttccgaag gtcttatggt caaacatgca gatcttttag
      721 tgtgtgatag taagaatatt gaaaaatata tccaagaaga ttatatacag tatcaaccta
      781 agacaaccta tatcgcttat gggacagata caacacgctc cacattaaat agcagtgatg
      841 aaaaagtgcg tgcttggttc aaggagaaaa atgtttctga aaacgagtat tacctagttg
      901 taggacgttt tgttccagaa aataattatg agtctatgat acgtggtttc ttggcgtcta
      961 gctctaagaa ggattttgtc ctaatcacaa atgtagaaca gaataaattt tataatcagc
     1021 tattagcaag tactggattt gataaagatc cacgagtgaa atttgttgga acagtctacg
     1081 accaagaact tctgaagtat attcgagaaa atgcttttgc ttacttccac ggacatgaag
     1141 ttggtggaac aaatccttcg ttacttgaag cattagcatc cacaaagtta aacttactac
     1201 tcgatgttgg ttttaaccgc gaagttgggg agcaaagtgc gatctattgg aaaaaagatg
     1261 agctttccca agtaatcgag aaagttgaac aatttgatgc aaaaattatt gatgagttag
     1321 atagacaatc aaatcagaga attgtggatt ttttcacttg ggaaaagatt gtcacagact
     1381 acgagaaatt atttaaaaaa tgaaaatatt acattatact ttaggatttc aaccccagcg
     1441 aacaggtggt ttggttaaat atgctgagga tttaatgcta gagcaaattg ctcaaggtta
     1501 tcaagtggct gccctatatc caggaagaat taagttcttt tcaaaaaaaa tagaaataat
     1561 taaagagacg tcaagacagt ttgagtgtta tgagctgctt aatagcttac ctatggcttt
     1621 atttggaggg atatccgatc caactgcttt cctgacacct tgtgacaaaa atgtttatcg
     1681 tactttttta gaaaaagtac aacccgacat tatacatatt cactcgttta tgggattgca
     1741 taaagaattc ctcgaaattg ctaaaagctt gaatattaga gtggttttta cgagccatga
     1801 ttattatgga ttagcacccg tacctcattt ctattttaat ggggtggatt atagtgataa
     1861 aagtacaaac ttaacatgga atattatgtc ttccaatgct ttaagtgtga aaaaactaag
     1921 actttttcag gtttcatttt atcctactat tcgtaaactt ttgaaactat tagggaaaaa
     1981 tccaaaatct aaaaagaatt tagttattcg agatgttatt gaggagcaag attatagtga
     2041 gcttcgatac tattataatg agatgtttca cttaatagat ggttatttgt ttaatagtag
     2101 acttgcaaag aaggtatatg agataaatga gattaagtca gctaatagta tagtattatc
     2161 tataacaaat agttcaatta atcatcatca gagattgaca actacaaata ataaaattag
     2221 agttgcttat attggtccag atgaagaata taaaggatat tttgattttg ttgactttgt
     2281 tgaaacttta gatcgagagt cctatgaagt ggcaacttat ggccatttac caaatgaaga
     2341 gtgtccttca ttcattgaac aaaaaggata ttttactaag gaaatgattg acagtgtcta
     2401 tgaaaatatt gatattctta ttgttccaag taagtggaag gaaacatttg gattgataac
     2461 agtagaagca ttatcctatg gggtaaatgt tttcgtgagt gaaaatgttg ggtcaaaaga
     2521 cttacttcca gaatcgcatg tttttaaaaa tcagaatgat ttaatagtta aatttttaaa
     2581 aaatgatata gaaaatacaa aattgaaaac tttagatgaa cactcgatag aagtgattca
     2641 gtattatgaa agagtgataa atgatagtta agagtttcat aaaaaagatt actggaaaaa
     2701 cagtagacat tcatccagat gtacctttgt cgtatatact acaaagaggt ataaactata
     2761 gtttcggact ctttcgtggg gttgtacgtg gaatcggatt tggtcaaaac gacaaacgtt
     2821 tattcattgg acaaggagtt tctatcttag caaagcgtaa attatttgtt ggaaaaaatg
     2881 ttcgaattgg taaaaaggtg tctatagatg cactgtcaaa agaaggaatc cattttgcag
     2941 ataatgtaaa gattggagat tattctcaaa ttattggcac aggttctata aaaaatatgg
     3001 ggattggctt gaaaatcggt aagaactcat ctttttcaga atatagttta tttggttctg
     3061 ctggaggtat cacaattggt gataatgtta tcgcaggtca gaatgttcgt tttcatgctg
     3121 aaaatcataa ttatagtgat ttagataaac tcattgttga acaaggggta tctcgaaagg
     3181 gaatttcagt aggtgataat tgttggattg gtgctggatc tgtttttttg gatgggtcca
     3241 gcattggttc aggttgtgtt gtagctgcaa attctgtaat tacgaaaaac tttccagata
     3301 acgttattgt tgctggagta ccagctaaaa ttataaaaat gaaatgatag gatgttaaat
     3361 gaggaaagaa attaaagctg tttatgcgat attaaattac aatacttggg aagatactgc
     3421 gcgtttagca cagaaagttg caacatttca gcatattcaa tctgtgataa tagtggataa
     3481 tctatcaaca gatgattcat accattattt aaaaagactt gagggagaga aaatttcagt
     3541 ttatcaaaca caaagaaatg gtggatattc tgttggaaat aattttgcag cacgaaaagc
     3601 ttataatatg ggagttgata ttcttttcat ttcaaatcca gacgttgata ttgatgaaaa
     3661 agattccttg atgattgcac aaaatttgta taaaaatagt tcatatgcac tgctatcagg
     3721 tatagaatat aatgctatga aggagataga tttgccaatt gtctggcatg aaaattccta
     3781 ttacgatgac ttacttgatt gtttgttttt aaccagaaaa tggcgaagaa aaaaagaaga
     3841 tatcaatttg tcaatgatta aatcatctat aatagatgta gatcttgtta aaggatcgtt
     3901 ttttgctgta agactgtctg attttcatga tgttggttat tttgatgaat ctgtatttct
     3961 tttttgtgaa gagagaatat tagctaaaaa gttacaaaaa gtaaataaaa aaataggtat
     4021 tctgcctgaa gcgaaatatt accacaatca ttcaacttct ataaatgaga gatataaaaa
     4081 gaaaaaagaa cagattaatt tattatataa gtctcgatac tactataatg taaagtataa
     4141 taatattagt tttgtcaaaa aactatgttt aaaatctgtc atgatgctat ccattttaga
     4201 atatataatc ttagatatga tgaatttatt atatacaaag actaactata agaaaattca
     4261 atagtttaat ctgaaatact tcttgtttca aagtggatga ttttagagca cggcaaaatg
     4321 tccttgaaaa agtttatttt ttcaaaggtt atcttgtggc aaattcagga gttagaacac
     4381 tttttttcgt caaatgctag cttgtttttt aggagataaa acagatttct aataagtttt
     4441 ttggcaaggt gaatgatggc tacattgtaa tgctttcttt gttacaactt agtcctaaga
     4501 taggctttaa aaacaggcga aaagcgagca tatgctttgg tagcttgtat gagtgcccaa
     4561 cgtagatgag gggaacccag tttgaccatt ttccctgcta aatcgatttg acctgactgg
     4621 taaatagaag gattcggtcc agcaaaagct tgtaattgat gaggcttatc gaagatatga
     4681 atacttcgaa tctcaactaa gataacgtca cctaaacgat tcccaagctc acttggttat
     4741 ccacgaactt tgtgccttgt taccttacac aatataaaac gtttatgcgc tatcaaactc
     4801 attaccaatt gaaacaaaaa gctgtggtta gagcctttca gaaatcgtca agcgattgga
     4861 ggaaatgaac taatccacag tggcttattc caagtgtacc acttggactt tagcagtagc
     4921 gaactacact aaatataata taaggagaaa taggtgagta aattaatatt gaacttgaaa
     4981 ttaaattctt ttttatttgt tataatttta cttttagttg ttgttactcc gactcatacc
     5041 ttaatttttg atgcactaat tccaacgaaa attgataatt tatggagaga gtttcttcta
     5101 gtaatttctt ttctatgtat tctaaagata aatgcaggaa aaattaaaat tggaaagcta
     5161 gggggcccaa taattgttat gggaggaatt ggtttagttt atactatctg ttcagatagg
     5221 ccatttacag ctttaaatct ttttagaatt tatatgtttc ctgctgtaat atattttata
     5281 ataataaatt gtaattttcg caaggagaga ttactgatat tgaagcaagc gcatgtatat
     5341 actgcttgca tattagcttt atggggaatt tttcaggcgt gggtgttaaa agatcagttt
     5401 ttgattaaaa taggatatcc ttcccaagga aattttctaa agagtacagc tttttatatt
     5461 ggtggttttt tcggtcaaca acgagttact agcacttttt ctgctcctaa tttagctgga
     5521 gtttattttg gaatatcttt gatcatttta ctctctatat ttgatactat aaagagtaat
     5581 agattggtat tgttttcaat tgtagcagct tttgttctta catttagccg tagtgctatt
     5641 ataagtactc ttgtaggaat cgtattcttt caacgtaaga aattgttttc aacgacgaag
     5701 attaatgtta tgaccttggt aatcttcccc ttaatttttt tgattgtttt agttatattt
     5761 tatctttatc ctgagaatgt tattataaat atgctctata gcagttattc ttcaacactt
     5821 aatttgacag attcttcagc ggttaagcat ttggaagact tatggcttcc tcttctaaaa
     5881 gttattgatt atcctttggg acttggattt ggtaataatg gcccaattgt tctgtcactc
     5941 tatcactcag caaatttagt agaatcttct atttatcttc ttgcatatga ttttggtatt
     6001 ttaggaatgt tcatatattt gttcccttat ttttatacga tttttgtata taaaaaatat
     6061 ttgcttagcg gtgctatatg ctgcttagtt cttataactt atctatttct tccaaatgta
     6121 gaaaattttg aaatcatatt ttttatttac cttttcattg ggatggatga actagcacta
     6181 tatacaaaag gaatgaatgt acatgaaatt gattaaatta ttgaaaaaat atataaattt
     6241 atacattagt agttcggtgc ctaagacaaa taaattgtta atttttggat catggttagg
     6301 tgaaaaatat gcagacaatc caagatattt gtttgagtat gtgattaaga atcgtcccga
     6361 tttaaaagct atatggatta caagtaatca agatgttttt aaagaattac agaagaaaca
     6421 atatctcgtg atgatggcag aggcacctga aactcggaaa attgttaaaa aagcaaagta
     6481 tatttttaca gcaactggta tttttgatat tggagaacaa aatgctaact tcgttggagg
     6541 tgcctatcta attaatctat ggcatggcat tcctttgaaa aaaatcatgt atgatgataa
     6601 acattcagca cttcacaaac gtagtaaact agtgacttgg gtagaaaaaa ttcctcttcg
     6661 aaactatttt gttatttcta ctagtacggc aataactcaa atttatcagt ctgcttttcg
     6721 cgtaaagaaa tctaatatat tagaacttgg tcaacctagg aatgactatt tttatgataa
     6781 gtctaatcca gtgagttcat taatacagga attaaaaagt aaaaatatta tcttatatat
     6841 gccgacacat cgcaatgaag gaaagaaaca aatagatcta gataagttaa tggatttaga
     6901 acacttaaat aattggtgtg aagaaacaaa gtcaattttt gtaattaaaa agcatttcta
     6961 tcattcaaaa gaaaaaacta tcgataagaa atattcttcg ataattgacg tgacaaatga
     7021 gaaggtggat gttcaagaat tactcaaatg ttcaaatgtt ttaataactg actactcaag
     7081 ctgctatatt gactacttat tgttgaatcg tccaattata ttttttaatt atgattatga
     7141 tgattacttg cgggtggatc gttcgctata ttttccatat gagaatgtta ctccagggga
     7201 gaaatgtcaa aactttgatg aattattagt gactttgcaa aatctatatg taggaaaaga
     7261 tgattataga gaagaacgtg aaaatatcaa aactttcttt tattctagtg aaactcagaa
     7321 gtcagtttct gaaaaaataa ttaatcacgt actaaatcta taactttatt ggaatctgtc
     7381 atgaaaaaaa tattaaataa gtattattct ttatctaatc ctgtaaaagc ttcaatttgg
     7441 tttactattt gcaatgttct tcaaaaagga atttcaatga ttactgttcc agtttttact
     7501 agagtattga caacggaaca atatggtgta tactctgtat atcaatcatg gtattcaata
     7561 attggagttt ttgcaacact aaacctatat tatggagtct ttaataatgg aatgattaaa
     7621 tacgagaaag ataaaaatgt ctttacttcc tctatgcagg gactaacaac aacagttaca
     7681 gctatatttt tactgatata tttaataggg attgattttt ggaactcgtt gttaggctta
     7741 cctacactac taatcttagt gatgttcttt gatttgtttt ttacccctgc ttattctttt
     7801 tgggttgctc gtcagagatt tgaatataaa tatagaaact tagtatttat cacatttatt
     7861 atagcgattg gaagtcctat tattggtatt tctgcagttg ttttatcgac ttataaagcg
     7921 gaggctagag ttatctcatt tgtgttagtt caatcttgta tagggttata tttctatatt
     7981 ttgaaccttt accgaggtaa acattttttt tgtaaaaaat attggctcta tgctttgaat
     8041 tttaatttac ctcttattcc tcattattta tcgcaatcag ttttaaacca atcggataga
     8101 ataatgatta attcaatggt aggaatggga gaagcagcaa tatatagtgt tgcttacagc
     8161 atatcaattt tgatgctttt agtgacaagt gctataaaca gctcttttat tccttacaca
     8221 tataagtgta ttcgagataa gaaatatact gaactaggga aaagtgcaaa tcttttaatt
     8281 actctggtag gcattggttc gattttaact atttctcttg gaccagaaat cattcaatta
     8341 tttgcaccaa aacaatatta cgaagcaata tggattattc ctcctgttgc tctgtcagta
     8401 tatttcatgt tcttatatcc catttttggt aacattgagt tttactttga agcaaatcat
     8461 tttgtaatgt gggcttcaat cggtggagca attatgaata tttttttgaa ttttattttt
     8521 ttaaaacact ttggatatat tgcggcaggc tatacaactt tattctgtta tattttattt
     8581 gcgttgggac attacatttt tatgaggaga gtactgtatc agaatctttc gggtatccaa
     8641 atttataatt ccagatatct tttatttttt tcgattcttc ttattattct aatgctacta
     8701 atcgtgatta tttatccatt tatactgatt cgttatctta ctatctcact catcctagga
     8761 ggatgttttt taaagaggaa aaagataatg agttcgatag ctatcattaa acaacagtga
     8821 ggtacaagaa tgacagaaac aaataaacag ttacatgaaa cattaatcga aattttagac
     8881 tttgtaaaag aaatttgtga aaaacatgaa ttaacttatt ttttggtctt tgggacagct
     8941 cttggagcaa aacgccattg tggatttatt ccatgggatg atgatgttga tattgctttg
     9001 ccacgagagc actacaatat atttatcgat gcattatctg agacggatca atctatattt
     9061 agtcttcaaa atgaagataa cgagccgaat tattttttac catttgctaa attaagaaaa
     9121 aacaatacga tttttattga gaagattctt gatgttgaat atgagaataa cggcatttac
     9181 attgatattt ttccactaga tttcgtagag aatccggact ctttcaattt taagattagg
     9241 agaactacat ttaattatat aaagcatatt ttaaaatttt caagttgcag aagtttttat
     9301 aaaaataaat acagtaatgt gcgttatttg attgaaaata ttatgagtat tcctaccctg
     9361 tttttttcaa ataggagatt attattctta gcgaatagtt taatttcatc aacgactaaa
     9421 gctgatttta ttggtcaata tgatcagaaa agtaacaaaa gagctattat gccttcaaac
     9481 tattattttc cacctaggtc cgctgtgttt gaaggaaaga cctatagtgt tcctgcgaaa
     9541 ttagaagatt atttaaaatg tttttatggc tcggattata tggaattacc accaatagaa
     9601 aaaagagtca ctcatcaacc aataacactt agattcgaaa aataatttgc tattctgtag
     9661 aaggagaata taatgaaaag agtaattaca tatggaacgt ttgatttact tcatcatgga
     9721 catatcaatt tattgcgacg tgcaaaagaa ttaggtgact atctagtggt agttgtttca
     9781 agtgatgaat tcaacctaat tgaaaaaaat aaagtttgtt atttcaatta tgagcataga
     9841 aaaagtctag ttgaagctat tagatatgtt gaccttgtta ttcctgaaac gagttgggaa
     9901 caaaaacgca gtgatgtcaa agagtatcat attgatactt ttgtgatggg ggatgattgg
     9961 attggagaat ttgattatct aaaagaagaa ggagttgaag tcgtttactt acctcggacg
    10021 aaagaaattt cgacaactaa aataaaaaaa gatttatcaa tgtaatattc gtaaataagc
    10081 attgttctgt tattgttctc gaatagacca ctgtattttt aagtagaaat gctaaaatcg
    10141 atagaaaaga gaaaaatgat aggacactac caatataggt aaaaaatatc gtaattagat
    10201 aaataagaaa ggtaccctac tatgaaaggt attattctag caggtggttc ggggacacgt
    10261 ttatatcctt tgactcgcgc tgcatcaaaa caacttatgc cggtttatga taaaccgatg
    10321 atttactacc cactttcaac attgatgttg gctgggatta gggatatttt gattatttcc
    10381 actccacagg atttacatcg attccaagag cttcttcaag acggatctga gtttgggctc
    10441 aaactttctt atgcagagca accaagtcca gatggtttgg cacaagcctt tatcattggg
    10501 gaagagttta tttctgatga tagcgttgcg ctgatcttag gtgacaatat ctatcacggt
    10561 tctgggcttt ccaagatgct acaaaaggca gcgagtaagg agtcgggagc aactgttttt
    10621 ggctaccatg tcaaggatcc agagcgcttt ggtgtggttg agtttgatca ggatatgaag
    10681 gctatttcta ttgaagaaaa gccagagcaa cctcgttcaa actatgcagt tacaggtctc
    10741 tatttctatg ataatgatgt agtagagatt gccaagagta ttaaaccaag tcctcgtggt
    10801 gaactggaaa ttacagatgt aaacaaggct tacctagatc gtggtgattt gtctgttgag
    10861 cttatgggac gtggctttgc ttggctggat actggaactc atgaaagttt actagaggct
    10921 tcacagtaca tcgaaacagt gcaacggatg caaaatgttc aggtagcaaa cttagaagaa
    10981 attgcttacc gtatgggcta tatcagtcga gaagatgtat tggccttagc ccaaccactt
    11041 aagaaaaatg aatacggaca gtatctgctc cgtttgattg gagaagcata gatgacagat
    11101 aattttttcg gtaagacgct tgcggcacgc aaggttgaag ctattccagg catgttggag
    11161 tttgatatcc ccgttcatgg agataatcgt ggctggttta aagaaaattt ccaaaaggaa
    11221 aaaatgcttc cacttggatt tccagagtct ttctttgcag aaggaaaatt gcaaaacaat
    11281 gtatccttct cacgtaaaaa tgtccttcga ggtctccacg cagagccttg ggataagtac
    11341 atctctgtag cagatggagg gaaagttctg ggttcttggg ttgatctacg cgagggtgaa
    11401 acctttggga atacctatca gacagtgatt gatgcgagta agggaatctt tgttcctcga
    11461 ggcgtagcta atggcttcca agttttatca gatacagtgt catatagcta tctggtcaat
    11521 gattactggg ctcttgaact caaacccaag tatgcctttg tgaactacgc tgatccaagc
    11581 cttggtattg aatgggaaaa tattgcagaa gcagaggttt cagaagcaga caaacatcat
    11641 ccactactta aggatgtaaa acctttgaaa aaagaagatt tggaataagg aaagaatatg
    11701 actgaataca aaaatattat cgtgacaggt ggagctggct ttatcggttc taactttgtc
    11761 cattatgttt acgagaactt tccagatgtt catgtgacag tcctagataa gttgacttat
    11821 gctggaaacc gcgcgaatat tgaggaaatt ttaggtaatc gtgttgagtt agttgttggt
    11881 gacattgctg atgcggagtt ggtagacaag ttggctgctc aagcagatgc tatcgttcat
    11941 tatgcagcgg aaagccacaa tgataattcg ctcaatgatc catcgccatt tattcatact
    12001 aacttcatcg gaacctatac tcttttagaa gctgctcgta agtatgatat tcgcttccac
    12061 catgtatcga cagatgaagt ttatggggat ctccctttac gcgaagattt gccaggtcat
    12121 ggtgaaggac caggtgagaa atttactgct gaaacaaaat acaacccaag ctctccgtac
    12181 tcatcaacca aggcagcctc agatttgatt gtcaaagcct gggtgcgttc ttttggagtc
    12241 aaggcaacga tttccaactg ttcaaataac tacggtcctt atcaacatat cgaaaaattc
    12301 atcccacgtc agattactaa catcctaagt ggaattaagc caaaacttta cggtgaaggt
    12361 aagaatgttc gtgactggat tcataccaat gaccattctt caggagtttg gacaatcttg
    12421 acaaaagggc aaatcggtga aacctacttg attggggctg atggtgagaa gaacaataag
    12481 gaagttttgg aacttatcct taaggaaatg ggacaagcta cggatgccta tgatcatgtg
    12541 actgaccgtg caggacatga ccttcgctat gcgattgatg ccagcaagct ccgtgatgag
    12601 ttggggtgga aacctgaatt taccaacttt gaagctgggc tcaaggcaac aatcaagtgg
    12661 tatacagata accaagaatg gtggaaagca gaaaaagaag ctgttgaagc caattatgct
    12721 aagactcagg agattattac agtataaaaa gcaggaaata gctgcttttt attgctatat
    12781 tgggaagagt tacatattag aaaggtctag agatgatttt aattacaggg gcaaatggcc
    12841 aattaggaac ggaacttcgc tatttattgg atgaacgtaa tgaagaatac gtggcagtag
    12901 atgtggctga gatggacatt accaatgaag aaatggttga gaaagttttt gaagaggtga
    12961 aaccgacttt agtctaccat tgtgcagcct acaccgctgt tgatgcagca gaggatgaag
    13021 gaaaagagtt ggacttcgcc atcaatgtga cggggacaaa aaatgtcgca aaagcatctg
    13081 aaaagcatgg tgcaactcta gtttatattt ctacggacta tgtctttgac ggtaagaaac
    13141 cagttggaca agagtgggaa gttgatgacc gaccagatcc acagacagaa tatggacgca
    13201 ctaagcgtat gggggaagag ttagttgaga agcatgtgtc taatttctat attatccgta
    13261 ctgcctgggt atttggaaat tatggcaaaa acttcgtttt taccatgcaa aatcttgcga
    13321 aaactcataa gactttaaca gttgtaaatg accagtacgg tcgtccgact tggactcgta
    13381 ccttggctga gttcatgacc tacctagctg aaaatcgtaa ggaatttggt tattatcatt
    13441 tgtcaaatga tgcgacagaa gacacaacat ggtatgattt tgcagttgaa attttgaaag
    13501 atacagatgt cgaagtcaag ccagtagatt ccagtcaatt tccagccaaa gctaaacgtc
    13561 cgctaaactc aacgatgagc ctggccaaag ccaaagctac tggatttgtt attccaactt
    13621 ggcaagatgc attgcaagaa ttttacaaac aagaagtgag ataagtagta gaatgatttt
    13681 ctagtctaat aaaagaggca gataatgaac tccaaaggag cttaagatgt acgcttatct
    13741 tgttgttggt gctggtctct ttggcgcata gctttggctc agtttctatt atcgctcaca
    13801 ccatccatca gaagtttaat ctgaaggtac ccaattatcg ccaagaagaa gattgggcta
    13861 ggatgggttt accaatcaca cgtaaggaaa tctctaattg gcatatcaag gcaagtcaat
    13921 actatttaga gtccctttat aagcttttac gagaaaagtt gttagaacaa cctcttcttc
    13981 ctgcggatga aacctcttat cgggttctag agagtgatag ccatctgacc tactattgga
    14041 cctttttgtc tgggaaagct gagaatcaag caatcacgct gtaccatcat ggtcagtgtc
    14101 gaagtggttt agtagtacaa gaattcctag gagattattc tggctatgtg cattgtgata
    14161 tgttgcggca gtaacttagg actttagtcc tctagttctg cctatgcgat agcagtccaa
    14221 ggtttaggag caaggcgacg ctaagcttgg taaactgcga accgctagaa gcttatcgtc
    14281 aactggaaga agctgcactt gttggatgtt gggcacatgt gagaaggaag ttttttgaag
    14341 cgacccccaa gcaagcggat aaatcatcct taggagctaa aggtttagct tattgtaatc
    14401 agttattttc cttggaaaga gactgggagg ctttgccagc tgatgaacga ctacagaaac
    14461 gtcaagaaga gctccaaccc ttaaaggaag acttctttgc ttggtgccgc cgtcagtcag
    14521 ttttagcagg ttcaaaacta ggaagggcaa ttgaatacag cctcaagtat gaagaaacct
    14581 ttaagactat tttgaaagac ggacatctgg tcctttccaa taatctagct gaatgctcca
    14641 ttaaatcatt ggttatggga cggagtaaaa gagtccagtg gactctttta gcctaagctc
    14701 agtttaaaaa agcgagggtg gttattttct caaagttttg aaggagctaa agcaagagct
    14761 attattatga gtttgttgga aacagctaaa cgtcatcaat taaatagaga gaaatatcta
    14821 ttctatcttc tagaatgtct tccaaacgag gaaactctcg taaacaaaga ggttttagag
    14881 gcttatttac catggactaa agttgtacaa gaaaagtgca aataagaaat ct
//
LOCUS       DBEROU010000049        13413 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.049, whole genome shotgun sequence.
ACCESSION   DBEROU010000049 DBEROU010000000
VERSION     DBEROU010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13413
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.049"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..1064
                     /locus_tag="ACL8CL_001794"
                     /pseudo
     CDS             1..1064
                     /locus_tag="ACL8CL_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436265.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1149..2351)
                     /gene="rpsA"
                     /locus_tag="ACL8CL_001795"
     CDS             complement(1149..2351)
                     /gene="rpsA"
                     /locus_tag="ACL8CL_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HGS1330995.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFVDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSLKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRAARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
     gene            complement(2513..2584)
                     /locus_tag="ACL8CL_001796"
     tRNA            complement(2513..2584)
                     /locus_tag="ACL8CL_001796"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2550..2552),aa:Gln,seq:ttg)
     gene            complement(2594..2674)
                     /locus_tag="ACL8CL_001797"
     tRNA            complement(2594..2674)
                     /locus_tag="ACL8CL_001797"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2638..2640),aa:Tyr,seq:gta)
     gene            complement(2724..2954)
                     /locus_tag="ACL8CL_001798"
     CDS             complement(2724..2954)
                     /locus_tag="ACL8CL_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HGS1330996.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAVEILIENYNLAK"
     gene            complement(3122..3766)
                     /gene="pcp"
                     /locus_tag="ACL8CL_001799"
     CDS             complement(3122..3766)
                     /gene="pcp"
                     /locus_tag="ACL8CL_001799"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016920 - pyroglutamyl-peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HGS1330997.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            complement(3781..4704)
                     /locus_tag="ACL8CL_001800"
     CDS             complement(3781..4704)
                     /locus_tag="ACL8CL_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0859 family membrane protein"
                     /protein_id="HGS1330998.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            complement(4701..5390)
                     /locus_tag="ACL8CL_001801"
     CDS             complement(4701..5390)
                     /locus_tag="ACL8CL_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0858 family membrane protein"
                     /protein_id="HGS1330999.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            complement(5455..6159)
                     /locus_tag="ACL8CL_001802"
     CDS             complement(5455..6159)
                     /locus_tag="ACL8CL_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1331000.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASLGYSIATRT
                     STTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSETTDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGSPVVSNVVPFSKPYSQVGIKGDPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKHIK"
     gene            complement(6215..7237)
                     /locus_tag="ACL8CL_001803"
     CDS             complement(6215..7237)
                     /locus_tag="ACL8CL_001803"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HGS1331001.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            complement(7369..9840)
                     /gene="parC"
                     /locus_tag="ACL8CL_001804"
     CDS             complement(7369..9840)
                     /gene="parC"
                     /locus_tag="ACL8CL_001804"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HGS1331002.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKNTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGAVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEDK"
     gene            complement(10041..10245)
                     /locus_tag="ACL8CL_001805"
                     /pseudo
     CDS             complement(10041..10245)
                     /locus_tag="ACL8CL_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014166.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
     gene            complement(10260..12203)
                     /gene="parE"
                     /locus_tag="ACL8CL_001806"
     CDS             complement(10260..12203)
                     /gene="parE"
                     /locus_tag="ACL8CL_001806"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HGS1331003.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KIGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            12339..12980
                     /gene="plsY"
                     /locus_tag="ACL8CL_001807"
     CDS             12339..12980
                     /gene="plsY"
                     /locus_tag="ACL8CL_001807"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HGS1331004.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDSLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            complement(13025..>13413)
                     /locus_tag="ACL8CL_001808"
     CDS             complement(13025..>13413)
                     /locus_tag="ACL8CL_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS1331005.1"
                     /translation="AYNLSCSPNLEQVQTMLEQAFTEKHYENTILHSDQGWQYQHDSY
                     HQFLEGKGIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKTFKSLNQLEQAIIDYI
                     DYYNNKRIKVKLKGLIPVQYRTKSFG"
BASE COUNT         3887 a         3018 c         2356 g         4152 t
ORIGIN      
        1 gatggtcgct gaaacttcta tcgtcaagaa gaatcatcaa attcctcgta ttatcaacca
       61 aaaaattgcg caaaagttga ttgagaagat ttctatgacc gatattgccc atcagctggc
      121 catttcaact tcaactgtca ttcgaaagct caatgattct cactttgagc atgatttttc
      181 gcgtcttcct gagattatgt cctgggacgt tgaaacagtc cggggagtga ctgtttcaat
      241 cgggagatgg agatgagctt tattgcgcaa gattttaaca atctcaatat catcactgtt
      301 cttgaaggta gaacacaagc tgtcatccga gatcactttc ttaaatatga tagagccgtc
      361 cgatgtcgcg tcaaaatcat tactatggat atgtttagcc cttactatga cttagctaaa
      421 cagcttcgct ttcaaatttc taggctcagg ctgaaacagt ctcccaggct gtttcactcc
      481 cgaatgctaa aatcgttctt gatcgctttc acattgtaca acatcttagc cgtgctatga
      541 gtcgtgtgca tgtccaaatc atgaatcagt ttcatcgaaa atcccatgaa tacaaggcta
      601 tcaagcgtta ctggaaactc attcaacagg atagccgtaa actgagtgat aagcgatttt
      661 atcgccctac ttttcgcatg cacttaacaa ataaagaaat tcttgacaag attttaagct
      721 attcagaaga cttgaaacac cactatcaga tctatcaact cttacttttt cactttcaga
      781 acaaagaccc tgagaaattt ttcggactca ttgaggacaa tctgaagcag gttcatccta
      841 tttttcagac tgtctttaaa acctttctaa agaacaaaga aaaaatcgtc aacgctcttc
      901 aattacctta ttccaacgca aaattggaag cgaccaataa tctcatcaaa cttatcaaac
      961 gaaacgcctt tggttttcga aactttgaaa acttcaaaaa acggattttt atcgctctga
     1021 atatcaaaaa agaaaggacg aattttgtcc tttatcgagc ttagctgact tcaacccact
     1081 acagttgaca aagagccaga aaaatccctt gtttagtaaa caaaggattt tgtgaatttt
     1141 caatttgatt aaagttcgat gtcaccaaac aaatcagcca ttgaaaatcc tgtttgtgtt
     1201 tctggaagtt cgaaatcacg cttttcttga cgtcttggac gacgtggacg agcagcacgt
     1261 ttttcttctt tttgtccttc ttcttgggct ggacgttctt caagagcttt aatagaaagt
     1321 gacacgcgtt ctgcatctgc gttaacttca agaactttaa cttgaacttc ttgaccaact
     1381 ttaagagctt cttttggatt ttcaatccgt ttgtgtgaaa tttgtgatac gtgaacaagt
     1441 ccatcgatac ctggcaatac ttcaacaaat gcaccgaagt cagtcaaacg tttaactgtt
     1501 ccttctacta catcaccttt agccaatttt tgctcaacgc catcccatgg tcctggtact
     1561 gttgctttaa gtgaaagtga tacacgtcct tcttcttcgt taagatcaag gattttcact
     1621 tcaatttctt caccaacagt tacaactgat tttggtgata cgttacgttc atgtgacaat
     1681 tcagtcaagt gaaccaatcc gtcaacacca ccaaggtcga cgaaagcgcc gaagcttgtg
     1741 atacgagcaa ctttaccagt tacaacatca ccaacagcca atttaccgaa tacttcagcg
     1801 cgagctgctg cagtagctgc ttcaacaact tcacgacgtg aaaggatgaa gcggttttct
     1861 ttagcgttaa cttctttgat tttagtatca aattcttgac ctacaaaacg ctcagcatta
     1921 cgtacgaaac gagtatccaa cattgaagct gggataaatc cacgaacacc ttcaaattct
     1981 actgaaagtc cacctttaac ggcacgcgtt cctttaacag taacaacttc ttcttcgcga
     2041 ccaacaagtt tgtcccatgc tttgcgagct tcaaggcgtt ttttagatac aaggtatgta
     2101 actgtatcag tatctttacc aactacttga cgaagtacaa gaacatccaa tacttctcct
     2161 actttaacaa agtcattgat atctgcatca cgatcgtttg tcaattcgcg aagagtcaag
     2221 acaccttcaa caccagttcc agagattgca acgttagctt gagtcgcatc aactgtcaat
     2281 acttcagcac taacaacatc accagtctca acttgactaa cgctatttag caaatcttca
     2341 aattcgttca tctaaaaaat cctccaacaa tcaagttttt cttaaacttg acatacttat
     2401 aatttttttc ctaagcaccc gcaaggacat gttcatatca ttcacgtctt tcaattataa
     2461 aaataaaata ccctaagata ttttgctttt tatcttgatt attacctaag aactggggta
     2521 gctggattcg aaccaacgca tgagggagtc aaagtccctt gccttaccgc ttggctatac
     2581 cccattaatg aaatggagag agagggattc gaacccccga acccgaagga gcggatttac
     2641 agtccgccgc gtttagcctc ttcgctatct ctcctacaat caacatggac tattatatca
     2701 tgaaaatttc aaaaaaacaa gacttatttt gctaaattat aattttcaat caaaatttcc
     2761 acagcttttt ccaatttttt ataaaaacta tcgacattcc cattctttat aatggcaaat
     2821 tgtccgtcta attcggcaat ttctccgata acttttttac cgatagtcaa tgtataacct
     2881 tcaaaactgt ctttaccaac attaactttg gcatccgcta cttgaatttc gattttttta
     2941 tctttcttgc tcatttcata cctctcttca ctttttctat tttacaagaa aattctaaaa
     3001 ctagcaagaa aaaactctat atcaggcttc tctgatatag agttttatgt aaacaacctc
     3061 aattgattta gcatagttaa tagttagtaa acaagcataa ttgactatcc tttttaggtt
     3121 atcagtgagt ctcaccgcct actaactgca aatccttatc tttgtagtct acaatagctt
     3181 caatagctac ctctatgcct cttacaatat cacatagact cattcctgca gtattcggct
     3241 tatttactac ttgttccatc atatatggaa tatgcatgaa acctgctctc atattaggga
     3301 attttttatc cactaaataa agagcttggt acatcaaatg attgcaaaca aaggttcctg
     3361 cactattgga tacaactgcc ggaagtccct gttttttgat agcttgtacc atcgctttga
     3421 taggtaaact actaaaatag gccgatgctc catcaatacg aatcggtgta tcaattggtt
     3481 gattgccttc gttatcaggt atgcgagcat catcttgatt aatagccact cgttcaggtg
     3541 ttaagccggt cctgccgcct gcttgtccaa tacaaagtac agcatctggt tgatatcgta
     3601 atatttctgc ctctaaaact tctgacgact tataaaaaac cgttggaatt tctacccagc
     3661 gaacttcagc cccattaatc tcagatggta ataattttac agcctccaaa gctggattaa
     3721 tcttttcacc tccaaaagga ttaaaacctg taaccaatat tttcatttta ttttccttta
     3781 ctaaaatgcg agaaagtaca ttaagaatat gtgaataaca atcattacta gagcaacacc
     3841 tgcttgagcc tttataacgc cattctgatc tttcatatcc atcaatgctg ctggtagagc
     3901 gttaaaatta gcagccattg gggtcaataa agtcccacaa taacctgctg tcatggcaag
     3961 agcaccagcc acaattggat tagctcccag agcaaataca aagggaactc caacacctgc
     4021 tgtaataacg gtgaatgctg caaaagcatt tcccataatc attgtgaata gaaccattcc
     4081 aagaacatag gccaaaactc ctataaagcg actatctgaa ggaacaatac cgctaatcag
     4141 atgagagata acatcaccaa cacctgctac agtaaaaata gcccccaaag cccctaataa
     4201 ttgaggaaca atcccacttg ttgaaacttg ctgagtcatt cgattatttt ctgataacag
     4261 actcttaggg tgactattgg taatcacaag aacagaaatt gtagcaaaca aggcggcaag
     4321 gctaatcgaa atcttgctaa attctggaat catttgcgct aagaccaacg caagtattgc
     4381 catcagcata actggaataa aaattttatt tttcaacctg ttagattcaa tattggcttt
     4441 catttcatct aaggatggca aggttccgat acggacttgc ttaaacaatg ttaacagcga
     4501 taataggatt acaataatac caatactcat atttggcata taggaaccac ctataaacgt
     4561 aatagacaat agagtccaaa atgcagatgt cccaagtcga actgggtttg ttttatcttt
     4621 ataactacaa taggctgtat ggagaaattg acaaccaatc acaatatagg tcaactctaa
     4681 tagttgcttt gccaactctg tcatttttgt tctcctcccc tagtcttttt tgatatcaat
     4741 tttttatcaa ataaataatt gtaaatcccc actacaataa gtgttataac agcaacaata
     4801 atagatgtag aagcaatccc tgcataattg ctttcatagc ctaactgatc taatgttccc
     4861 cctatcaaga ggactccccc agcacctaca aacgtatttt gagcaaagaa atttccaaaa
     4921 ttttcattcg cagccgcacg cgcttttatt gtctcatctt caacctctgt taactttcta
     4981 cctaattgag actctgcagc tgcttctccc ataggttgaa ccaaaggtct gacaaactga
     5041 gggtgtcctc ctagacgaat tgaaaagaaa ccagctaact ctcgaataaa gaaataaact
     5101 gtatagaagt ttccaactgt cagaccttta atctttcgaa tcaaatcgat tgatcgttgc
     5161 ttgagtccaa aggtttctga cagccccaca agaggcaagg taaccataaa aatcgtgagc
     5221 actcgctgat tgctaaattc ttttcccaaa atctccaaaa attcaacgag agaaacacct
     5281 gaaactaaag ctgtaaccaa accagctaag actactgttg caattgtatc aaattttaaa
     5341 ataaaaccca caacaatgat tgctattcct attaatctaa tccactccat atcaaactcc
     5401 tttatattca aaatgacagt atttttaaaa ttttatcaag atcaatacca ttccttattt
     5461 aatgtgtttt tctagttctt tttggtattt gctattggat tccaattttt ctttttgcca
     5521 ttttttaaaa acctcgttat attcttttgt tgtaacaata tctttttgca atttcattcc
     5581 tttaaagata tatggatccc ccttaatacc aacttgtgag tatggttttg agaatggtac
     5641 tacgttactt acaactggag aaccaccaga tgaagctgtt ggcatcaata atgaactatc
     5701 tgtcgaccaa gcttgagctt tggcatattt ttcatatctc ttctctaggt cagtggtctc
     5761 agaaacagca tcttctaaca atttcttata tttatccaaa ccaggtttag ctacaacatc
     5821 cttatctttt cctttcgtaa taccaaggtg tttcatggca gaaccagatt ttggatccat
     5881 aatattcaag taagacgctg gatcttgata gcttggagcc catcctgtac tattcaaatc
     5941 atagtctttt tgagaaggag caacattgcc gtatttatca ttttccatca aaccatcaat
     6001 aacatttcca ataacgtctg tcgtcgatgt tcgagtcgct atactgtagc ccaatgatgc
     6061 tggatctact gcatagacat aagaaaatgt tgtcggtgca tctgcttctt tatcagtttt
     6121 tccacaagcc actaaaatag ctgacgtgct caggaccact cctgctgtta agagccactt
     6181 tttctatttc ataaaaaatc tcctttggtt tattttaatc tacttttaca atccaacctt
     6241 ctggcgcttc aatatcgcca aactgaatac ccgtcaattc attatataat ttacgcgtca
     6301 caggacctac ttctgtttca ctatagaata catggaaatc atcaccatgt tgaatacctc
     6361 caattggaga aataaccgct gctgtaccac aggcacctgc ctctacaaaa cggtcaagat
     6421 tatcaattgg aacatcaccc tcaataggag ttaatcccaa gcgatgttct gccaaataaa
     6481 gcaaggaata cttggtaata gatggcaaga tagatggact caatggtgtt acaaattcat
     6541 tatcagctgt aattccaaag aagttagctg atccgacttc ttcaatcttt gtatgagttg
     6601 atgggtccag atagataaca tctgagaaat gacgtgactt ggccattttt cctggtaaga
     6661 gacttgcagc atagtttcca ccaaccttag ccgcacctgt accatttggt gctgcacggt
     6721 cgtactcatc ctgaatcaag aagttggttg ggaccaaacc acctttaaag taatttccaa
     6781 ctggcatagc aaagatggtg aaaatgtact cttctgccgg ttttaccccg ataatatctc
     6841 cgacaccaat caaaagaggg cgaagatata aggttccacc tgttccgtat ggtggtacgt
     6901 attcttcatt cgcacggaca actgctttac aagcttctac aaacatgtct gtcggaactt
     6961 gtggcatcaa gagacggtca catgtacgtt gcagacgttt agcattttca tcaggacgga
     7021 acagttgaac actgccatcc ttagtacgat aagctttcaa accttcaaat gcttgttgtc
     7081 catagtgaag acttggagaa gactctgaaa tatgcaaagt tgcatcctct gtaagctctc
     7141 cttgatccca ttgtccattt ttgaaatgag caagatagcg ataaggtaat ttcatatagg
     7201 aaaaaccgag gttttcccaa tcaatcgtta ctgtcatatt ccactcctta ttctaaaaac
     7261 ctatttctta tattctacac tatttttcta aaatagcaag tatattttgt aattttcaga
     7321 aaatttctcc aataaaaacc agctcttaga actgattctt catttcactt atttatcttc
     7381 agtaactact tcctgaagat aagcgtcaaa aacttcttca tctgaaatcg tgtcagaaat
     7441 gaagcttcca ttgctagtgc gttctgacaa gttcaagtct tgcaatcggc tttcatagat
     7501 tgttccttta ttggattgga caagcagagt ttggtcgttc acatccactt ccgtactgaa
     7561 gaaatcgcca acaaatcctt gctctgcaac tgctcctgcc aagaagacac gatgcggttt
     7621 gtttttcaac tcacgcaaga cttgtaatcc tcgtttggca cggctggttg ctagaatttc
     7681 ctcaatagaa acacgtttca agcttccacg ctgggtcaag aggtagaagg acgaagtatt
     7741 acagataaag ccagattgga ggacatcatc ttctttcaaa ttcatagcct tgacacctgc
     7801 tgccttagca ccgacaaccg gaacctcttc gatattgaaa cgcagggcat aaccattttg
     7861 actaaccaag acaacatcat ctagtttaat cggagccact gctacaatct gatctgtatc
     7921 gtctttgagc ttagcatact tgacagactt agatctatag gtccgccatg gagtgaattc
     7981 ttttcgctct acccgtttga tttgaccaag gcgagtcact gcaaagtagg ttgtcgcatc
     8041 gtcaaactga tccagtactt ccacataaag gatttcttca ttcgtttcaa agtttgtgat
     8101 ggtttggctc agatgctctc cgatgtcctt ccaacgaata tctgccaact catggattgg
     8161 tctgtagatg acatttccaa gacttgtgaa catcaagagg tgctgggttg tcttggcaga
     8221 ttgaacaaaa atcaaacggt catcatcacg cttgccaatt tcttccaagg tggaagccgc
     8281 aaaggaacgt ggactggtac gcttgatgta acctgccttg gtcacgctga cgtaggtatc
     8341 ttcctcagcg ataagactag ctgtatcaat ctcaattgct ttcgcagtgt cttctaaaga
     8401 actcaaacga ggagttgcaa atttcttctt gacctcacga agttctttct tcatgagatt
     8461 gtacatagtc ctttcatcac cgataatagc cgccagcata gcaatcttct cacgaagctc
     8521 tgcttcttct tcctgcaaga caaccacatc ggtattggtc aaacggtaca gttgcaaagt
     8581 tacgatagcc tcagcctgtt cttccgtaaa atcatagcta actttgaggt tttccttggc
     8641 gtccgcctta ttctcagaag cacggataag agcaatgact tcatccaaaa tcgaaatcac
     8701 acgaatcaaa ccttcgacga tatggagacg tttctcagcc ttttctttgt caaagcgtga
     8761 acgcgccaaa atcacttctc gacggtgagc gatgtagcta gacaggattg gaacaatccc
     8821 aacctgacga ggtgtgaaat tgtcaatcgc caccatatta aagttgtagt tgatttgtag
     8881 gtcggtgtac ttaaataagt agttgagaac aagctcagta ttagcgtctt tcttaagttc
     8941 gatagcgata cgaagaccat cacggtcaga ctcatcacga acctcagcaa tcccagctac
     9001 cttgttatta acacgaacat catcgatttt cttgactaga ttggccttat tgatttcata
     9061 aggaatctca gtaataacga tttgttcctt accacctttt agcttttcaa tttcagtctt
     9121 ggaacgaaca accacgcgcc ctttcccagt ctcataagct ttcttgattt catcacgacc
     9181 ctgaataata gcccctgtag ggaagtctgg tccaggcaag aattccatga gtttatcaat
     9241 ctttgcagtt gggtggtcaa tcatgtaaac tgcagcatct atgacctcag ctaaattatg
     9301 gggaggaatg tctgtggcat aaccagccga aatcccagtc gaaccattga ccaagaggtt
     9361 tggaaaggct gctggcaaga ccgttggttc tttctccgta tcgtcaaagt tccatgcaaa
     9421 aggaactgta tttttctcga tatcctgaag aaggtaacct gcaatttcag acaaacgtgc
     9481 ctcagtataa cgcatagccg caggaggatc tccgtccata gaaccgttat taccgtgcat
     9541 ttcaactaga atctcacgat ttttccagtt ctgtgacata cgaaccatgg catcatagat
     9601 agaagaatcc ccgtgtgggt ggaaattccc catgatgttc ccgactgact tggccgactt
     9661 acggtagctc ttgtcaaaag tattgctatc cttattcata gaataaagaa tacggcgctg
     9721 aaccggcttc aacccatcac gaatatctgg caaagcccgg tcttgaataa tgtacttgga
     9781 gtagcgacca aagcgctctc ccatgatgtc ctccagggac atgttttgaa tgttagacat
     9841 aagatacaaa gcccataaaa taccaagtga aaatagaaaa ttcttgaagt aagcaaactc
     9901 acaagagaat ttatcttttt cacacagtat ctagggcgtg ttcaactcct ttcaaagaat
     9961 gtagagtagg tttttatgca gtaaaagata ttttacggga attcctcccg tgttcagtta
    10021 cgataagtaa ccaaactatc ctgtttgtat ttttcaatat gaaaatctgg ttttccaaaa
    10081 ttagtcttag tttgtgtctt aaccgctccc ttaagcgcct ctttgagata agcactcata
    10141 gcagattctt cattaataat cctgcaattt tttcaaacca agattttcaa actgcttttt
    10201 cacatagtca ttcacatccg actctaattt ccagtttact aacatattat tttctttcat
    10261 taaaacactg tcgcttcttc tagcgtaaac ttgacattat cttcaatcca tttacggcgt
    10321 ggttctacct tatctcccat gagaacattg acgcggcgtt cggcgcgcgc taaatcttca
    10381 attgtgacac ggatgagggt acgtgtttct gggttcatgg ttgtttccca gagctggtcc
    10441 gcattcatct caccaagtcc tttgtatcgt tggagggtag cgcctttacc gaactgttta
    10501 cggagttctt ctagttctcc gtccgtccaa gcgtaggcca cttcttcttt cttgccttta
    10561 cctttggaca tcttgtaaag aggtgggagg gcaatataga catgacctgc ctcgactagc
    10621 ggacgcatgt aacggtagaa aaatgtcaag agcaaggtct ggatatgggc accgtcggta
    10681 tccgcatcgg tcatgataat gatcttatca tagttggcat cttcaataga gaagtctgct
    10741 ccaacacccg caccaatggt ataaatcatg gtattgatct cttcattttt gaggatatcc
    10801 gccatcttgg ccttggctgt attgataacc ttaccacgaa gaggtagaat agcctggaac
    10861 ttgcggtcac gaccttgttt ggcagaacca ccggcagagt ccccctcaac tagatagagt
    10921 tcattcttag caggattctt agattgggct ggggtcaatt tcccagacaa caagccctta
    10981 tctttcttgt ttttcttccc atttcggctc tcatcacgcg ccttacgtgc tgcttcacga
    11041 gcatcacggg ccttgatagc cttgcggatg aggttagaag ctaattcccc attttccata
    11101 aggaaaaagg tcaacttatc agccactatt ccatccacaa ctgggcgagc tagggggctt
    11161 cctagtttat ccttggtctg tccttcaaac tgcaagtgtt cttcaggaac taagatagaa
    11221 agaacggccg ctagtccctc acgatagtct gaaccttcaa ggtttttatc tttttccttg
    11281 agaagacctg ttttacgtgc atagtcattc atgaccttgg taatggcaga cttgagtcct
    11341 gtctcgtgcg ttccaccgtc cttggtgcga acgttattga caaaggatag aatgttatct
    11401 gagaatccgt cattgtactg gagggctact tccacttgaa aaccattgtc ttccccttca
    11461 aagtaaagaa ctggcgtcaa gatttcctta tcttcgttga gataagaaac aaaatcttgt
    11521 actccattct catagtggaa ctcaatcgct tcatctgttc gcttgtccgt taaagacaag
    11581 gtcacatttt tcaagagaaa ggctgattca ttaaggcgct ctgaaatggt attgtacttg
    11641 aaatctgtcg tagaaaagat agtcgcgtca ggcataaaag taactttggt gcctgtttta
    11701 gacttgggtg ctgtaccgat tttcttcaaa gtcgtgacag gttttccacc attttcgaaa
    11761 cgttgcttgt aaactgcgcc atcacgggta atttcaactt ctaaccagct agaaagggcg
    11821 ttaacaacgg aagaacccac tccgtgaagt ccacctgatg tcttatagcc accttgaccg
    11881 aatttccctc cggcatgaag aatggtaaag ataacctcaa cagttggaat tcccatagcg
    11941 tgcatacctg tcggcatccc acgtccatgg tcttgaaccg ttagactacc gtctttattg
    12001 atggttacat cgatacgatc accaaaccca gacaaggctt catcgactgc attatcaacg
    12061 atttcccaaa ctaggtgatg aagaccagcg ccatcggtcg atccaatata catccctgga
    12121 cgttttcgga ccgcatccaa cccttctagc acctgaatag catcatcatt ataattgtta
    12181 atattgattt ccttttttga cacaaggaac ctcctattcg ttcatcttta ctattctaca
    12241 ggttttccaa ggattttgca aaatttttct ttctccgatg tgacaatttt agcagagatt
    12301 ctctgctttt ctttcccaat tcatgatata ataggagtat gattacaata gttttattaa
    12361 tcctagccta tctgctgggt tcgattccat ctggtctctg gattggacaa gtattctttc
    12421 aaatcaatct acgcgagcat ggttctggta acactggaac gaccaatacc ttccgcattt
    12481 taggtaagaa agctggcatg gcaacctttg tgattgactt tttcaaagga accctagcaa
    12541 cgctgcttcc gattattttt catctacaag gcgtttctcc tctcatcttt ggacttttgg
    12601 ctgttatcgg ccataccttc cctatctttg caggatttaa aggtggtaag gctgtcgcaa
    12661 ccagtgctgg agtaattttc ggatttgcgc ctatcttctg tctctacctt gcgattatct
    12721 tctttggagc tctctatctt ggcagtatga tttcactgtc tagtgtcaca gcatcgatcg
    12781 cggctgttat cggggttctg ctctttccac tttttggttt tatcctgagt aactatgact
    12841 ctctcttcat cgctattatc ttagcacttg ctagtttgat tatcattcgt cataaggaca
    12901 atatagctcg tatcaaaaat aaaactgaaa atttggtccc ttggggattg aacttaaccc
    12961 atcaagatcc taaaaaataa aatgccagtt ctgtgaactg caccccaaaa gttagacaat
    13021 taatttatcc aaaggattta gttctgtatt gcacagggat aagtcctttt agttttacct
    13081 taattcgttt attgttgtag taatcaatat agtctataat agcttgttcc aattgattaa
    13141 gtgatttaaa tgttttctca taaccgtaaa acatttccga tttcagaatc ccaaagaagg
    13201 actccatcat accattgtct gggctgttac ccttacgtga catggatgct tgaattccct
    13261 taccctcaag aaactgatga taagaatcgt gttgatattg ccagccttgg tcactatgga
    13321 gaatcgtatt ctcgtagtgc ttctctgtga atgcctgttc caacattgtt tgtacttgtt
    13381 ctaaattagg cgaacaagaa agattatagg cga
//
LOCUS       DBEROU010000050        13403 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.050, whole genome shotgun sequence.
ACCESSION   DBEROU010000050 DBEROU010000000
VERSION     DBEROU010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13403
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.050"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            148..804
                     /locus_tag="ACL8CL_001809"
     CDS             148..804
                     /locus_tag="ACL8CL_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HGS1331006.1"
                     /translation="MPTALGYVSIGLACGTIGAPYVTPVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVAAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFGLDFALVGMFIGI
                     FASQFQIMQRRIPVRNLLIILAVVAVSFFLLLTVVSQSLAVLFATLLGCSMGVVLDGQ
                     "
     gene            794..1117
                     /locus_tag="ACL8CL_001810"
     CDS             794..1117
                     /locus_tag="ACL8CL_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HGS1331007.1"
                     /translation="MVSKYLLLAVIFSGLVTWILRMIPFILVKYKGLPAIVERFLKFL
                     PVSIIFALILSSVVTGKVGSLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAI
                     LRLVF"
     gene            1221..2051
                     /locus_tag="ACL8CL_001811"
     CDS             1221..2051
                     /locus_tag="ACL8CL_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HGS1331008.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2205..3059
                     /locus_tag="ACL8CL_001812"
     CDS             2205..3059
                     /locus_tag="ACL8CL_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HGS1331009.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNAITIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            3159..4532
                     /locus_tag="ACL8CL_001813"
     CDS             3159..4532
                     /locus_tag="ACL8CL_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HGS1331010.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4525..5586
                     /locus_tag="ACL8CL_001814"
     CDS             4525..5586
                     /locus_tag="ACL8CL_001814"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HGS1331011.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSELSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSNPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5588..6280
                     /locus_tag="ACL8CL_001815"
     CDS             5588..6280
                     /locus_tag="ACL8CL_001815"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HGS1331012.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6310..6879)
                     /locus_tag="ACL8CL_001816"
     CDS             complement(6310..6879)
                     /locus_tag="ACL8CL_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HGS1331013.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIAFFFLGTKHGAGSFLPGIICGLLADEVAHLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVTPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            7014..7628
                     /locus_tag="ACL8CL_001817"
     CDS             7014..7628
                     /locus_tag="ACL8CL_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HGS1331014.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLAGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7606..9276
                     /locus_tag="ACL8CL_001818"
     CDS             7606..9276
                     /locus_tag="ACL8CL_001818"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1331015.1"
                     /translation="MKNWRQNRMKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSR
                     EKEAQRVAQRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQ
                     LERKASPYISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDF
                     KPAGNSFAIPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMAVTVTSPFDTEIF
                     HMGETVDKESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLT
                     LRQTFANYQKQVVDLVDSIQAIAQGQEGLRIDTLEKDQELLLIAETTNDMLDRLEKNI
                     HDIYQLELSQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLR
                     NNISDERETLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVE
                     NYFAHGVDHRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQKYFEHQ
                     ASYSDQRQSIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9288..10574
                     /locus_tag="ACL8CL_001819"
     CDS             9288..10574
                     /locus_tag="ACL8CL_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS1331016.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISDQPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVPITKQLFIQFVMDVF
                     HLFEHLKADDLTDIVKTIHAIQSFDELVPYIKETLSSFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10626..11159
                     /locus_tag="ACL8CL_001820"
     CDS             10626..11159
                     /locus_tag="ACL8CL_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1331017.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11237..11707
                     /gene="nrdI"
                     /locus_tag="ACL8CL_001821"
     CDS             11237..11707
                     /gene="nrdI"
                     /locus_tag="ACL8CL_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HGS1331018.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(12000..13262)
                     /locus_tag="ACL8CL_001822"
     CDS             complement(12000..13262)
                     /locus_tag="ACL8CL_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HGS1331019.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
BASE COUNT         3924 a         2513 c         3084 g         3882 t
ORIGIN      
        1 tcagttctat ctgcaacctc aaaacagtgt tttgagcaac ctgcggctag tttcctagtt
       61 tgctctttga ttttcattga gtattattaa gacaatatga ggagaaagat gaaagaaaaa
      121 ggattttagg agggggcgca ggcagccatg ccaacggctc ttggctatgt cagtatcggc
      181 ctggcctgtg ggactatcgg tgcgccctat gtgacacctg ttgagatggg cttgatgagt
      241 ctctttgttt atgctgggag tgcccagttt gccatgttgg cactgattgt ggttcaagct
      301 cctgtggcag ctattgctat gacggttttt ctaatcaact tgcgtctctt tttgttgagt
      361 ttacacgcat cgacttattt ccgtcatacc agtctttggt acaatatcgg tatgtctagt
      421 atcttgacag atgagaccta tggcgttttg atgggtgaat tggcccatac agacaaggta
      481 aatcctatgt ggatgcacgg aaacaatctt aacagctatg tggcttggtt tgtggggaca
      541 gtagtcggaa cggctctggg tggcctgcta ccaaatccag aaatctttgg cttggatttt
      601 gccctggttg ggatgtttat tggtattttt gcttcgcaat ttcagattat gcaaagacgg
      661 attcctgtcc gcaatctgct cattatccta gcagttgttg cggtgtcctt ctttttgctc
      721 ttgacagtgg tgtctcagtc actagctgtt ctgtttgcga cgctacttgg ttgtagcatg
      781 ggggtggttt tagatggtca gtaagtatct tttattagca gttattttct ctggcttggt
      841 gacttggatt ctccgtatga ttcccttcat cttagtcaag tataagggct tgcctgcaat
      901 cgttgagcgt tttttgaagt tcttgcccgt ttccattatc tttgccttga ttctttcaag
      961 cgtagtgaca ggtaaggttg ggagccttcc tcaaattaaa tggctagact tcttagccgt
     1021 ctttccaaca gcttgggtag cctttcgcta ccgcaatcta gtcggaacag ttctctttgg
     1081 agtggtcttg attgccatct tgcgtttggt cttttaaatt acccataaaa aaaacttatc
     1141 acagagatag atatcatata atggcgtaaa tgctcttttt ctgttaagat tataaggtat
     1201 tctattttgg aggaaatgac atgaaaaaaa tcgttaaata ctcatctctt gctgccctag
     1261 gacttgttgc tgcaggtgtg cttgcggctt gctcaggggg tgctaagaaa gaaggagaag
     1321 cagctagcaa gaaagaaatc atcgttgcaa ccaatggatc accaaggcca tttatctatg
     1381 aagaaaatgg cgaattgact ggttacgaga ttgaagtcgt tcgcgctatc tttaaagatt
     1441 ctgacaaata tgatgtcaag tttgaaaaga cagaatggtc aggtgtcttt gctggtcttg
     1501 acgctgaccg ttacaatatg gctgtcaaca atcttagcta cactaaagaa cgtgcggaga
     1561 aatacctcta tgccgcacca attgcccaaa atcctaatgt ccttgtcgtg aagaaagatg
     1621 actctagtat caagtctctc gatgatatcg gtggaaaatc gacggaagtc gttcaagcca
     1681 ctacatcagc taagcagtta gaagcataca acgctgaaca cacggacaac ccaactatcc
     1741 ttaactatac taaggcagat ttccaacaaa tcatggtacg tttgagcgat ggacaatttg
     1801 actataagat ttttgataaa ataggtgttg aaacagttat caagaaccaa ggtttggaca
     1861 acttgaaagt tatcgaactt ccaagcgacc aacaaccgta cgtttaccca cttcttgctc
     1921 aaggtcaaga tgagttgaaa tcatttgtag acaaacgcat taaagaactc tacaaagacg
     1981 gaacgcttga aaaattgtct aaacaattct tcggagatac ttatctacca gcagaggctg
     2041 atattaaata atttcttgaa atcatccatt ctgagtaaga tgggtgattt ctcagttttt
     2101 agggatatag ttttaaacta tatatctgat tatctaataa caatttgtga aatcaaacaa
     2161 attgtgagat actagtacgg tattattttt aaggagaaag aatcatgaaa atcaaaaaat
     2221 ggcttggcct agtagccctt gctacagtcg caggtttggc tcttgcagct tgcggaaact
     2281 cagaaaagaa agcagacaat gcaataacta tcaaaatcgc aactgttaac cgtagcggtt
     2341 ctgaagaaaa acgttgggac aaaatccaag aattggttaa aaaagacgga attaccttgg
     2401 aatttacaga gttcacagac tactcacaac caaacaaagc aactgctgat ggcgaagtag
     2461 atttgaacgc tttccaacac tataacttct tgaacaactg gaacaaagaa aacggaaaag
     2521 accttgtagc gattgcagat acttacatct ctccaatccg cctttactca ggtttgaatg
     2581 gaagtgccaa caagtacact aaagtagaag acatcccagc aaacggagaa atcgctgtac
     2641 cgaatgacgc tacaaacgaa agccgtgcgc tttacttgct tcaatcagct ggcttgatta
     2701 aattggatgt ttctggaact gctcttgcaa cagttgccaa catcaaagaa aatccaaaga
     2761 acttgaaaat cactgaattg gacgctagcc aaacagctcg ttcattgtca tcagttgacg
     2821 ctgccgttgt aaacaatacc ttcgttacag aagcaaaatt ggactacaag aaagcacttt
     2881 tcaaagaaca agctgatgaa aactcaaaac aatggtacaa catcattgtt gcgaaaaaag
     2941 attgggaaac atcacctaag gctgatgcta tcaagaaagt aatcgcagct taccacacag
     3001 atgacgtgaa aaaagttatc gaagaatcat cagacggttt ggatcaacca gtttggtaat
     3061 aagaaacagg gaggtgggag agagaattcc acctcttgct tttgtataga gcacggagtg
     3121 taaagaagac tattcgttca tagaaaggta gagagaatat ggtttttcct agcgaacaag
     3181 aacagattga aaaatttgaa aaggatcatg tagcccagca ttattttgag gttttgcgta
     3241 ccttgatttc taagaaatca gtctttgccc agcaggttgg actcaaggaa gtcgcaaatt
     3301 atctgggtga gattttcaag cgtgttggag ctgaagtgga gattgatgag agctatacag
     3361 cgccctttgt catggcacat ttcaagagtt cgcgtccaga tgccaagacc ttgattttct
     3421 ataaccacta tgacactgtg ccagcggatg gggatcaggt ctggacagag gatcctttta
     3481 cgctttcggt ccgcaatggc ttcatgtatg ggcgtggagt tgatgacgac aagggtcata
     3541 tcacagctcg cttgagtgct ttgagaaaat atatgcagca ccatgatgat ttacctgtca
     3601 atatcagctt tatcatggag ggagcggagg aatcggcttc aacagaccta gataagtatt
     3661 tggaaaagca tgcagacaaa ctccgtgggg cggatttgtt ggtctgggaa caagggacca
     3721 aaaatgcctt ggaacagctg gaaatttctg gtggcaataa ggggattgtg acctttgatg
     3781 ccaaggtaaa aagcgctgat gtggatatcc actcgagtta tggtggtgtt gtggaatcag
     3841 ctccttggta tctcctccaa gccttacagt ctcttcgtgc tgcggatggc cgtatcttgg
     3901 ttgaaggctt gtacgaagaa gtacaagagc ccaatgaacg agaaatggcc ttgctagaaa
     3961 cttatggtca acgaaaccca gaggaagtta gtcggattta tggattggag ttgcctctct
     4021 tacaggagga gcggatggcc tttctaaaac gtttcttttt cgagccagcg cttaatatcg
     4081 aaggaatcca gtctggttat caaggtcagg gtgttaagac tattttacct gcagaagcca
     4141 gtgccaagct agaggttcgt ctggttccgg gcctagaacc gcatgatgtt ctggaaaaaa
     4201 ttcggaaaca gctagacaaa aatggctttg ataaggtaga attatactat accttgggag
     4261 agatgagcta tcgaagcgat atgagcgcac cagccattct caatgtgatc gagttggcca
     4321 agaaattcta tccacagggc gtttcagtct tgccgacgac agcggggaca ggacctatgc
     4381 atacggtctt tgatgcccta gaggtaccaa tggttgcatt cggtctagga aatgccaata
     4441 gccgagacca cggtggagat gaaaatgtgc gaatcgctga ttattacacc catatcgaat
     4501 tagtagagga gctgattaga agctatgagt agagatatta tcaagttaga tcagatcgat
     4561 gtgacttttc accaaaagaa gagaaccatt acagcggtta aggatgtgac cattcacatc
     4621 caagaagggg atatctacgg aatcgttgga tattctggag cagggaaatc aacccttgta
     4681 cgggtgatta acctcttgca aaaaccatct gcagggaaaa ttaccattga cgacgatgtg
     4741 atttttgacg gcaaggtgac cttgacggca gagcagttgc gtcgtaaacg tcaagatatc
     4801 ggaatgattt tccagcattt taacctgatg agccaaaaga cagcagagga gaatgtagcc
     4861 tttgccctta aacactctga actcagcaag gaagaaaaga aggctaaagt agctaagttg
     4921 ttggacttgg ttggtttggc agatcgtgct gaaaactacc cttcacaact atctggaggg
     4981 caaaaacagc gtgtggcaat tgcgcgtgcc ttggccaatg atccaaaaat cttgatttca
     5041 gacgagtcaa cttctgccct tgatccgaag acaaccaagc agattttggc cttgttgcaa
     5101 gatttgaacc aaaaattagg cttgactgtt gtcttgatta cgcatgaaat gcagattgtc
     5161 aaagacattg ccaaccgtgt tgcagttatg caggatgggc atttgattga agagggtagt
     5221 gtgcttgaaa tcttctcaaa ccctaaacaa cctttgactc aagactttat ctcaacagct
     5281 acaggtattg acgaagccat ggtcaaaatc gagaagcaag aaatcgtgga acacttgtct
     5341 gaaaacagtc tcttggtgca actcaagtac gctggagctt caacagacga gccacttttg
     5401 aatgaattgt acaagcatta ccaagtaatg gctaatattc tctatgggaa tatcgaaatt
     5461 ctcgatggta ctcctgttgg agaattggtg gtggttttgt caggtgaaaa agcagcgttg
     5521 gcaggtgccc aagaagccat tcgtcaagca ggtgtacaac taaaagtatt gaagggagta
     5581 cagtaagatg gaatcattga ttcaaaccta tttaccaaat gtctataaga tgggttgggc
     5641 tggtcaggca ggctggggaa cggctatcta cttaactctt tatatgacag ttctttcctt
     5701 cattatcgga ggcttcttgg ggctagtggc aggtctcttt ctcgtcttga cagcgccagg
     5761 tggtgtcttg gagaataaag tcgtattctg gattttagac aaaattacct caatttttcg
     5821 tgcggttccc tttatcatcc tcttggcaat cttgtcacca ctttctcact tgattgttaa
     5881 aacaagtatc gggccaaatg cagcccttgt cccactttct tttgcagtct ttgccttctt
     5941 tgcccgtcag gtgcaggttg tcttggctga attggatggc ggtgtcattg aggcggctca
     6001 agcgagcgga gcgactttct gggacatcgt gggtgtttac ctatcagaag gtcttccaga
     6061 tttgatccgt gtgacgactg tgaccttgat ttcccttgtt ggggaaacag ctatggccgg
     6121 tgcggttgga gctggtggta tcggtaacgt agccatcgct tatggattta accgctacaa
     6181 tcacgatgtg accatcttgg caaccatcat tatcattttg attatctttg caatccaatt
     6241 cttgggagat ttcttgacta agaaattgag ccataaataa aaaagagccg tgtggctctt
     6301 tttaactgat cagattttct gggcaaattt tttactcaag gcttgtccaa tcaaggcacc
     6361 cactagggct ccgatgacaa tacttgcgat aaatagaagg acagttccag ggtttggagt
     6421 gaccatgata cggtcgatat attcttggga ttttcctctt gccagaagag tagccatata
     6481 ggctttgggc gcaatccaca taagcaagat tggtcctgtt gtactaaagg cgaaaataat
     6541 gaaagaaagg aagttctttg ttttgtcctt gtattttcct aaatgagcta cttcatctgc
     6601 taggaggcca cagataattc caggaaggaa ggaaccagca ccgtgtttag ttcccaagaa
     6661 aaagaaggca atgacaaggc cgatagtggt aatggctcca aagcgcggaa cttttgcgac
     6721 taggatcata tagacgctac cgccgacaag ggcagtaaag gcaggcgcat aaaacatgtt
     6781 tccagtttgg tcaaagagat tgcccaaaag gacaccaatc cccatgcaga ggaagtaaag
     6841 aactgcaaaa agcagtgtag ttaagataga ttttttcatg aattgtctcc tgataatttt
     6901 ttcacaattc tcattgtacc acggtttggt gggattgtaa atggatacta gaagtttttg
     6961 aaaacgcttg aaatatgata taatggtttc atagttattt ttaggggaat atcatgggta
     7021 gatttttaga ctttgtcttt aatcgtttct ttttagggat gattgcgaca gccttctttt
     7081 ggctattaac tttagcagga gggattatcc ttggtctagc gccggctagt gccaccttga
     7141 tgagcttata tgcagaacat ggttatagct ttcgggaata cagtttgaag gaggcttggt
     7201 ctctttacaa gcaaaatttt gtctcaagca acctgatttt ctatagcttt ttaggtgtgg
     7261 gtctagtttt gacctatggt ttgtatctct tggtgcaatt gcctcatcag accattgttc
     7321 atttgattgc gacccttttg aatgtcctag tagttgccct gatctttttg gcttatacag
     7381 tatctttaaa attacaagtt tattttgcct tgtcctatcg aaatagtctc aaattatcct
     7441 tgattggcat ctttatgagt ctagcagctg tggccaaggt tctccttggg actgtgctac
     7501 ttgtagcaat tggttactat atgcctgccc tgctattttt tgtaggaatt gggatgtggc
     7561 atttctttat cagtgatatg ttggaacctg tctatgaaat catccatgaa aaattggcga
     7621 caaaatagaa tgaagcagtt ttggctacat acgcttctaa gaacctatag ttcagtgatg
     7681 atcattatca ttgcgagttt tgcaatctta ctctcttacg ctgactggga ttcacgtgaa
     7741 aaggaagccc agagagtagc ccagcgtgta actgctagaa cagtgagtga aattgaatat
     7801 taccatagag agtcaaccca gatagctcag gctttagttg aaaaccaagc tcgtattgag
     7861 ggaatctata aatactttag ccttagcatg ccagactatt tttactggca attagagcgg
     7921 aaagcttcgc cttatatatc agtctctctg tatgaaaatg ttgatgacct ctatgttcga
     7981 aatgattttg taactggggt ggccattgct tttcaagatt acaaggaagt ctatgtttct
     8041 actaaagaca aacgtagtgg agaaaaaatc agggctgagg atttcaaacc agcaggaaat
     8101 agttttgcca ttccagtgtc agatccagtg tcagatcaag acttaggagt gatttacatc
     8161 tccttggatc ctgctgtttt ataccatgcc attgataata ctagaggtca tactccgatg
     8221 gcagtaacag tgacctcacc ttttgatacg gagatttttc atatgggtga gacagttgat
     8281 aaggagagtg aaaattggct agttggctta acttctcatg gttatcaggt tcaggtggca
     8341 gttcccaaaa actttgtttt acaaggaacg gtgaccagct ctgctttgat tgtgggcttg
     8401 agccttctct ttattgtcat tctttatctg actttgaggc agacctttgc taattatcaa
     8461 aagcaggtag tggatttggt ggattccatc caagctattg cccaaggaca agaaggtctt
     8521 cgcattgata cgcttgaaaa ggatcaggaa ttgctcctaa tcgcggagac gaccaatgat
     8581 atgttggatc gattggaaaa gaatatccat gatatttacc agttagaact cagtcaaaaa
     8641 gatgccaata tgcgggcctt gcaggcgcaa atcaaccctc acttcatgta taatacgctg
     8701 gagttcttgc gtatgtacgc agtcatgcag agtcaagatg agttggcaga tatcatttat
     8761 gaattcagta gtctcttgcg taacaatatt tccgacgaaa gagagaccct cctcaaacag
     8821 gaattagaat tttgccgtaa atacagctat ctctgcatgg ttcgctatcc caagtccatt
     8881 gcctatggtt tcaagataga tccagagtta gagaatatga agattcccaa gtttaccttg
     8941 caaccgctgg tagaaaacta tttcgcgcat ggtgttgacc acaggcggac agataatgtg
     9001 attagcatca aggctcttaa acaggatggg tttgtggaaa ttttggtggt cgataatggt
     9061 agaggaatgt cggctgaaaa gttggcaaat atccgagaaa aattaagtca gaaatatttt
     9121 gaacaccaag ccagctacag tgatcaaagg cagtctatcg ggattgtcaa tgtacacgag
     9181 cgttttgtgc tctattttgg agaccgctat gccattacta tagagtctgc agagcaagcc
     9241 ggtgttcagt atcgtattac aattcaagat gagtagaaag ggagaaaatg tataaagtat
     9301 tattagtaga tgatgagtac atggtgacag aaggtctgaa gcgtttgatt ccctttgata
     9361 agtgggatat ggaggtcgtc gcaacagcca gtcatgccga tgaagctcta gaatatgttc
     9421 aggaaaatcc tgtcgatgtc atcatttccg atgtcaatat gccagacaaa acagggcttg
     9481 atatgattcg ggagatgaaa gagatcttac cagatgctgc ctatatcctg ctctcaggtt
     9541 atcaggagtt tgattatgta aaaagagcaa tgaaccttag tgtggtggac tatttggtca
     9601 agcctgttga taaggtagag ctgggaaatc tgctggagaa gattgcaggt cagctcggcg
     9661 agagagggaa gaaaagtcag actcttagtc aagaattaga cgaggctgga tttgttagtt
     9721 atttagggga taaggagaat tggtggatag gtctatccaa ggaaaaacaa ggttccttca
     9781 ccattcccta ctatgtcttg ggccaagact ggcagatttt catttctgac caacccctag
     9841 atggtttagt cgttacacct tttgaagctc cttatcaaga acactttgaa cgctggaagc
     9901 tgaatgctga gaaaaccctc ttttacggtt ctgtaaattt gcagcagtct gagagtctct
     9961 ttgcctatta cgaaccgatt tatagggtta tcattcaggg aaatctcaat caaatcgtag
    10021 aagagttaaa tctcttggag aaggtagttc ttgaaaatac gccgcgtgtt ccgattacta
    10081 aacagctttt tattcagttt gtcatggatg ttttccattt atttgaacat ctcaaagctg
    10141 atgacctgac agacatcgtc aaaaccattc atgctattca atccttcgat gaattggttc
    10201 cttatatcaa ggaaactctg agcagctttt tcggtcaata ccgtatgaat gaaaatgtgg
    10261 tcagtgtgct ggaagtcatt ggtcgtgatt accaaaaaga gctttccctc aaggatatca
    10321 gtaaggctct ctttatcaat cctgtctatc tagggcagtt gattaagcgt gaaaccgatt
    10381 cgacttttgc agagttacta aacaaacaac gtattaaggc tgcccaacaa cttttgcttt
    10441 caactagtga cagcatcgaa gatatttgtt atgctgttgg ttacagtaac cttggatatt
    10501 tctataaagt tttccgaaaa ttgtgcggaa aatcgccaaa agcctaccga aaacaggtag
    10561 aaactatact ataagatttg tattccttta caaaaggtgc tataatatta tcaggaggtc
    10621 ctatcatgaa aaagaaacca atttatctat gggtcttgct aatcttgtct gcccttattt
    10681 cagttccgtc tctgtttgga attgtgagtc ccttgcctag taaagaagcc cttcgtgctg
    10741 ctcagaaaca agttgcaggg gtcaatgctc agcaattaga agaccagctt aattatacct
    10801 atagagtagc agaagcatct cattctattt ttaatgttgc tttgattgtg ctatctacta
    10861 ttttagtggt cgtagcgatt gtcttccttg ttcgtaaaaa tttgcaatat gcaaactata
    10921 cttacgttgg ctatgttttg ttagctatta ttggttcgat ttatggctat gttggtttac
    10981 aagacgctgt gcagttggtt caagatgaga gcatgcgttt gacagtgagt attggctcaa
    11041 aagctgttag cattttctat atcgtcatca atgttctctt cctagccctt gtcttctata
    11101 agatgtggcg acaacagaaa gccttggcag aagaagagga aacagaagag cttacctaag
    11161 tattgacaaa aaagaactat caagatacaa tcttggtagt tgttttttga ttatagataa
    11221 aatcatggag gtacatatga agacaatttc tttagtttat attagtttga gtggcaacac
    11281 tgaaagtttt gtgacgcgct tgaaagacta tctcttgtcc cagtacaaag ggattgaggt
    11341 tcaaaagatt catatcaagg atttggtcaa ggaaggcaaa aatttctatg aaatggacca
    11401 tccctatgtc gcttttttgc cgacctatct cgaaggtggg aatggcgtgg ataacggaga
    11461 tgttgagatt ttgacgacac cggtgggaga ttttattgcc tatggtaaca atgctagtaa
    11521 gtgttttggt gtggttgggt caggaaatcg taactttaat aaccaatact gcctaacagc
    11581 caagcaatac agtcaacgct ttggcttccc tgtattggct gactttgaaa tgcgaggcat
    11641 gctggaagat atcaaacatg tcgcagctat tatcgcagat ttgtatgagt tggaaaagga
    11701 gaattaatac tcaatgaaaa tcaaagagca aactaggaag ctagtcgcag gttgctcaaa
    11761 gcacagcttt gaggttgcag atagagctga cgtggtttga agagattctc gaatagtata
    11821 aagttcttat aagaggtggt tgaagttgtt caactgtttt ttgagtataa acagtcttta
    11881 agaacgtaaa aaagcataag ttcaggtctt caaaaccttg ttcttatgct ttttatcata
    11941 gaaatatttg ctgaattttc tcttgaaatg aaaccttatc ttcatctcgc gcttgtccct
    12001 taccctgctt ttccgattgc gagtgcgtag ataaagaaga tggcgaagcc aaagaggaaa
    12061 atcaatcccg caatggcaag gtgtttgagc caagaaggaa gatttttgtt ttcacgcgtt
    12121 accaaggcgt aattcaaaag agcaaagaaa ggtgttgtca ggaaagatcc aatcatggca
    12181 aagcggagca tggttgaaac ctgaccagcg aagaacttga taatgacgat accgatgata
    12241 gcagtgatgg tcatccagat gttcaaagat ttacgattgt cctctttttg actgattagc
    12301 agtcggagag attcctgatt aacgcgagaa tagccatcga taacagttat aactgttcca
    12361 aagatacaga ggaaggcaat aaaggtaatc aagtaacggg accattcgcc aagaacagag
    12421 gcatacatgc ccacgaattg agagatgtat ttggctgaag cagcttcaac cgcctgccct
    12481 gtaggatact gaatcagtgc tcccagtgcc acaaagaaga cggctaggat agctgttcca
    12541 atataaccag tgttaaagtc aaacagagcg tcttctgtgt taaagttgac ggtctttctc
    12601 ttttcagctg accaaagtga attgatggct gaaatttcaa taggagccgg catccatcct
    12661 aggagggaga cgatgaaggg cagagctgcc atttgccaag gtgtcttctc gacaaaatca
    12721 gaactgtatt ctggatgctt gaccgccgca atgataactg caagaacagt cgcaatggtt
    12781 aaggcagaca taatccattt gaccatgccg tctaaaagtt tgtagcctcc aaagagtagc
    12841 atagcccaaa tgattgcaac gagaatgagg gaccactgag taatgctaag tccaatcatt
    12901 gggaaggcac tggcgatgat agctgagcac agaatggcaa caccagccgt gttgaccata
    12961 gccgaaaaga cattgagaat aaagaaaatc cagagataga gttttccttt ttcggcataa
    13021 ccttcaacca aagtctttcc agtatcagct gtgtattcag caccaaaacg gaaaaatgga
    13081 tatttaaaga cattggctaa gatgaccaag agaagtagag accaaccgta agaaccgcca
    13141 gcttgagttg aggatacaat gtgggaacct ccaacagcgg cagttgccat taggattcca
    13201 ggtcccattg cttttagctt gcttgcccag gttgattgat ttaaggaaat agcttgcgac
    13261 atgataaata ctcctttttg aattttcaga ataatctgta tatgcaatct atattctaca
    13321 aaaaattttg gaaaaatgca agaatatttt gaaaaaagat agaaaattta caaagaaaat
    13381 ctgaaataac tgttatgata tag
//
LOCUS       DBEROU010000051        13346 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.051, whole genome shotgun sequence.
ACCESSION   DBEROU010000051 DBEROU010000000
VERSION     DBEROU010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.051"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..426
                     /locus_tag="ACL8CL_001823"
     CDS             <1..426
                     /locus_tag="ACL8CL_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HGS1331020.1"
                     /translation="DNQEQLFDQVASLLEEREIVTPTYREALITREKSFPTGLDMEFL
                     GKDLPNVAIPHTDIVHNLAEKVVVVRLEKPVTFHNMIAPDKEVEVSLLFFIINNSSSS
                     QTNILAQLMDFFTGNGHLEDLSKISEPEALYAYIAEATA"
     gene            464..765
                     /locus_tag="ACL8CL_001824"
                     /pseudo
     CDS             464..765
                     /locus_tag="ACL8CL_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
     gene            773..1576
                     /locus_tag="ACL8CL_001825"
                     /pseudo
     CDS             773..1576
                     /locus_tag="ACL8CL_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1767..4058
                     /gene="pcrA"
                     /locus_tag="ACL8CL_001826"
     CDS             1767..4058
                     /gene="pcrA"
                     /locus_tag="ACL8CL_001826"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HGS1331021.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTAYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDSDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            4101..4781
                     /gene="radC"
                     /locus_tag="ACL8CL_001827"
     CDS             4101..4781
                     /gene="radC"
                     /locus_tag="ACL8CL_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HGS1331022.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(4778..5467)
                     /locus_tag="ACL8CL_001828"
     CDS             complement(4778..5467)
                     /locus_tag="ACL8CL_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGS1331023.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(5485..6126)
                     /locus_tag="ACL8CL_001829"
     CDS             complement(5485..6126)
                     /locus_tag="ACL8CL_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HGS1331024.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(6209..6424)
                     /locus_tag="ACL8CL_001830"
     CDS             complement(6209..6424)
                     /locus_tag="ACL8CL_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGS1331025.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGKKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(6432..6779)
                     /locus_tag="ACL8CL_001831"
     CDS             complement(6432..6779)
                     /locus_tag="ACL8CL_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HGS1331026.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(6784..7899)
                     /locus_tag="ACL8CL_001832"
     CDS             complement(6784..7899)
                     /locus_tag="ACL8CL_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HGS1331027.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLKAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(7909..8868)
                     /locus_tag="ACL8CL_001833"
     CDS             complement(7909..8868)
                     /locus_tag="ACL8CL_001833"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGS1331028.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(8981..9550)
                     /locus_tag="ACL8CL_001834"
     CDS             complement(8981..9550)
                     /locus_tag="ACL8CL_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HGS1331029.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            9678..10349
                     /locus_tag="ACL8CL_001835"
     CDS             9678..10349
                     /locus_tag="ACL8CL_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HGS1331030.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGVKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            10333..11151
                     /locus_tag="ACL8CL_001836"
     CDS             10333..11151
                     /locus_tag="ACL8CL_001836"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HGS1331031.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLEKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIAHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            11148..12044
                     /locus_tag="ACL8CL_001837"
     CDS             11148..12044
                     /locus_tag="ACL8CL_001837"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS1331032.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVTIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            12088..13062
                     /gene="pta"
                     /locus_tag="ACL8CL_001838"
     CDS             12088..13062
                     /gene="pta"
                     /locus_tag="ACL8CL_001838"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HGS1331033.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
BASE COUNT         3959 a         2545 c         2852 g         3990 t
ORIGIN      
        1 gataatcaag aacaactctt tgatcaggta gcaagcttgt tggaagaacg agaaattgtc
       61 actccaactt atcgtgaagc cttgatcacg cgtgaaaagt catttccaac tggtttagat
      121 atggaatttc taggaaagga cttgccaaat gtagcgattc ctcacacaga tattgttcat
      181 aatctagctg aaaaagtggt ggttgttcga ttagaaaaac cagtaacttt tcacaatatg
      241 atagctcctg ataaggaagt agaagtatcc ctactcttct ttatcattaa caattcaagt
      301 tcaagtcaaa caaatattct ggctcagttg atggactttt tcacaggaaa tggacatctt
      361 gaagatttat caaaaatttc tgaaccagaa gccctttatg cttacattgc tgaagcaacc
      421 gcttaatctt gtctattaaa aaataaaatc ggaggaaatc caaatgatta aaattcttgc
      481 tgcctgcggt gaggtgttaa ctcgagttac caaattaaaa gtgctctaga agaagaactt
      541 tcaaaccgtg gttatgatgt tcactgtgat gcagtcatgg tgaaagatgt aaacgaagac
      601 cttatgaaag gttacgatat ctttacacca atcgctgcaa cagatcttgg ttttgaacca
      661 ggtatcccag ttatcgaagc tgggccaatc ttattccgta taccagcaat gagcgctcca
      721 gtatttgaca atattagact ccctgcgaaa caaaacatgg tatagtagtt ctatgaatga
      781 tgaagcaagt aaacaactaa ctgatgcacg atttaagcgt tttgttggtg ttcagcgcac
      841 gacttttgaa gagatgttag ctgtattaaa aacagcttat caacttaaac acgcaaaagg
      901 tggacgaaaa cctaaattaa gcctagaaga ccttcttatg cccactcttc aatagtgcga
      961 gaatatcgaa cttatgaaga aattgcggct gattttggta ttcacgaaag caactttatc
     1021 cgtcggagcc aatgggttga aataactctt gttcaaagtg gttttacggt ttcaagaact
     1081 cctctcagtt ctgaggacac ggtaatgatt gatgcgacgg aagtaaaaat caatcgccct
     1141 aaaaaaacaa ttagcgaatg attctggtaa aaagaaattt cacgctatga aggctcaagc
     1201 gattgtcaca agtcaaggga gaattgtttc tttggatatc gctgtgaact atagtcatga
     1261 tatgaagttg ttcaaaatga gtcgtagaaa tatcgaacaa gctggtaaaa tcttggctga
     1321 cagtggttat caagggctca tgaagatata tcctcaagca caaactccac gtaaatccag
     1381 caaactcaag ccgctaacag ctgaagataa agcctataac catgcgctat ctaaggaaag
     1441 aagcaaggtt gagaacatct ttgccaaagt aaaaacgttt aaaatatttt caacaaccta
     1501 tcgaaatcat cgtaaacgct tcggattacg aatgaatttg agtgctggta ttatcaatca
     1561 tgaactagga ttctagtttt gcaggaagtc tattgaggta ttgagctagt ttatgaaaaa
     1621 attgggtgaa aagtcgagtg ttttagaaac ccacagtgta gtattctagt ttcaatccac
     1681 tatattttgc tactccccgt aaagtttcta ttttccctga tttctgatat aatagaaata
     1741 ttgacttcaa gagtaaggaa gagaagatga acgcattatt aaatggaatg aatgaccgtc
     1801 aggctgaggc ggtgcaaacg acagaaggtc ccttgctaat catggcaggg gctggttctg
     1861 gaaagactcg tgttttgacc caccgtatcg cttatttgat tgatgaaaag ctggtcaatc
     1921 cttggaatat cttggccatt acctttacca acaaggctgc gcgtgagatg aaagagcgtg
     1981 cttatagcct caatccagcg actcaggact gtctgattgc gaccttccac tccatgtgtg
     2041 tgcgtatttt gcgtcgcgat gcggaccata ttggctacaa tcgtaatttt acaattgtgg
     2101 atcctggtga acagcgaacg ctcatgaaac gtattctcaa acagttgaac ttggacccta
     2161 aaaaatggaa tgaacgaact attttgggga ccatttccaa tgctaagaat gatttgattg
     2221 atgatgttgc ttatgctgcc caagctggcg atatgtatac gcaaattgtg gcccagtgtt
     2281 atacagccta tcaaaaagaa cttcgtcagt ctgaatccgt tgactttgat gatttgatta
     2341 tgctgacctt gcgtctcttt gatcaaaatc ctgatgtttt gacctactac cagcaaaaat
     2401 tccaatacat ccacgttgat gagtaccaag ataccaacca cgctcagtac caattggtca
     2461 aactcttggc ttcccgtttt aaaaatatct gtgtggttgg ggatgcggac cagtctatct
     2521 acggttggcg tggtgctgat atgcagaata tcttggactt tgaaaaggat taccccaaag
     2581 ccaaggttgt tttgttggag gaaaattacc gctcaaccaa aaccattctc caagcggcca
     2641 acgaggttat taaaaataat aaaaatcgcc gtcctaaaaa tctctggact caaaacgctg
     2701 atggggagca aatcgtttac tatcgtgccg atgatgagct ggatgaggct gtatttgtag
     2761 ccagaaccat cgatgaactt agtcgcagtc aaaacttcct tcataaggat tttgcagttc
     2821 tctatcggac taatgcccag tcccgtacaa ttgaggaagc cctgctcaag tctaacattc
     2881 cttataccat ggttggcgga accaaattct acagccgtaa ggaaattcgc gatattattg
     2941 cttatctcaa ccttattgct aatttgagtg acaatattag ttttgagcgt attatcaacg
     3001 agcctaaacg tggaattggt ctaggtacag ttgagaaaat ccgtgatttt gcaaatttgc
     3061 aaaatatgtc tatgctggat gcttctgcta atattatgtt gtctggtatc aagggtaagg
     3121 cagcccaatc tatctgggat tttgccaata tgatgcttga tttgcgggag cagctagacc
     3181 acttaagcat tacagagttg gttgagtccg tcctagaaaa aacaggttat gtcgatattc
     3241 ttaacgccca agcgactcta gaaagcaagg cacgggttga aaatatcgaa gagtttcttt
     3301 ctgttacgaa gaactttgat gacaccacgg atgtgacaga agaggaaact ggtctggaca
     3361 aactgagtcg tttcttaaat gacttggctt tgattgccga cacagattca ggtagtcagg
     3421 agacatcaga agtgaccttg atgaccctgc atgctgccaa aggtctcgaa tttccagttg
     3481 tctttttgat tgggatggaa gaaaatgtct ttccacttag tcgtgcgact gaagattcag
     3541 atgaattaga agaagagcgc cgtctagcct atgtaggtat cacgcgtgca gagaaaattc
     3601 tctatctgac caatgccaac tcacgcttgc tttttggtcg taccaattat aaccgtccga
     3661 ctcgttttat taacgaaatc agttcagact tgcttgagta tcaaggtctg gctcgtcctg
     3721 caaatacaag ctttaaggca tcatatagca gtggtagtat ttcctttggt caaggtatga
     3781 gtttggctca ggctcttcaa gaccgtaaac gcggtgctgc cccaaaatca atccagtcaa
     3841 gcggtcttcc atttggtcaa tttacagctg gcgcaaaacc agcatctagc gaggcaaatt
     3901 ggtccattgg tgatattgct ctccacaaga aatggggaga gggaaccgtt ctggaagttt
     3961 caggtagcgg tgctaggcag gaattgaaaa tcaatttccc agaagtaggt ttgaaaaaac
     4021 ttttagccag tgtggctcca attgagaaaa aaatctaatt ttccatcctt ctcacgaata
     4081 ataaagtgag gaggattttt atgtacagta tttcattcca agaagattca ctattaccaa
     4141 gagaaaggct ggccaaggaa ggagttgaag cgcttagtaa ccaagagttg ctagctattt
     4201 tactcaggac aggaacacgt caagctagcg tttttgaaat tgcccaaaaa gtcttgaaca
     4261 atctttcaag cctaacggat ttgaaaaaaa tgaccctgca ggaattgcag agtttgtctg
     4321 gtattgggcg tgttaaggcc atagaattac aagctatgat tgaactgggg catcgtattc
     4381 acaaacacga gactcttgaa atggaaagta ttctcagcag tcaaaagttg gccaagaaga
     4441 tgcagcagga attaggggat aaaaaacaag agcacctggt ggcactctat ctcaatactc
     4501 aaaatcaaat catccatcag cagaccattt ttatcgggtc tgtaactcgt agtatcgctg
     4561 aaccgcgaga gattcttcac tatgcaatca agcatatggc gacttctctt atcttggtcc
     4621 acaatcatcc ttcaggagcg gtagcgccta gccaaaatga tgatcatgtc actaaacttg
     4681 ttaaagaagc ctgcgaattg atggggattg ttctcttgga ccatttgatt gtctctcatt
     4741 ctaattactt tagttatcgt gaaaagacag atttaatcta aagttcatta acgacatagt
     4801 caaagagttt tttatctttg ggacgatttt caaaaagaag ttctggatgc cattggacac
     4861 cgagaaaggc gacatcatcc gtactcatga cagcctcaat gataccatct ttaggatcat
     4921 gagccacaac ttttaaattt ggtgctaagt ccttgatgct ctggtggtgg aaggagttga
     4981 tatgagagat ttctccatag atttcttgga gaacggtatc tggttctgtt accaagcgtt
     5041 gagttgtgta ctcaacagaa gaatcctgcc aatggtcttc gatatcttgg tacaaagttc
     5101 cacccatggc aacgttaaag agttgggtac cacggcagac agagaaaatg ggctttttct
     5161 gtttaatagc ttccttgatg agggccagtt cgaagatatc tctttgaagg tgatagtcat
     5221 cactatcaat ggttttgggt tcgccataaa attttggatc gacattttgc ccacctgtca
     5281 agatgagctt gtcaatcaaa ctgatatagt ggcaggccat ttcttgatca ccaatcggta
     5341 ggatgatggg aatccctcca gcatctttaa cgccttcaac aaagcctttt gctgcgtagc
     5401 tcatcatgat gtcatcatct ggatgagttt tttcgtttcc tgtaatccca ataactggtt
     5461 ttttcataaa atgattttcg ctttctaatc ctcttttcgc atgaagtaga ggagggtttg
     5521 gagttcactt gtcaaatcga catactgaac gaccacgtct tttggtaaat gcagatggac
     5581 tggtgaaaaa ctgagaattc ctttcacacc agcatcaacc aagagattag caacctcttg
     5641 tgacttgacg ctgggaacag ttaggatagc agtcttcaca tcagcatcct tgattttatc
     5701 cttgatctga gaaatcccgt aaatgggaat cccgtcagga gtttgggtac cgacttcagg
     5761 atggtcgtct aggtcaaagg ccatgataat cttcatcttg ttacgttcgt ggaagcggta
     5821 gtggagaagg gcatggccca tatttccaat accaaccagc atgacattgg taatagagtt
     5881 gtcattgagc aaatcggcaa aaaatgtcat tagttttttg acatcatagc caaaaccacg
     5941 acgaccaagt tcaccaaaat aggaaaaatc acgacgtacg gtcgctgaat caataccgat
     6001 agcctctgca atttgcttag agttggcacg ttcaatcttt tctgcatgaa atctcttaaa
     6061 aattcgatag tagagagaga gtctttttgc tgtagctttt ggaatagcaa actgtttatc
     6121 tttcacaaaa tcacaacctt tctattcttc tattttatag aaacattgtg aaaaaatcaa
     6181 caaaaataag aaaaaactaa gaaaaatctt agttttgatg taaaaaatct gcatgagata
     6241 gaaaacggta gaggtctccg accagcccct gataaacttt tttgccccta aaagtcagag
     6301 aagtcacata aagtgtatct ggtaaggtta cacatcctga caaagtcaac atgagagcct
     6361 catgatcctc atacttgaga gtacgctcta catgatagca gtccttatag gtcagttcaa
     6421 acattttggc tctatctttc cgattttgta aagacaccac gttctaccaa gctatccatg
     6481 aggaagtaga atttttcctg atgaatatgg tggtcttctg atttgaaaat atcaactaga
     6541 cgaaggccaa acttgtcagt gatattgatt ttagcccctg taagttcctt gttaatgatg
     6601 attttgagtt ggaagccttc accgctgttt ggcacttttt ccaaaaggcg agtcagttca
     6661 tagttaccaa ccttagtttc aaaaaaggtg ttatctttga gggtgaattt tttaacagaa
     6721 gggctaagag tgtaatcgta acgacaattt tttaactgaa tgattttttc aaatgccata
     6781 tggctaacct ccgataattt cttttaaggt ttttgcgagg gtttgtaggt cttcaacggt
     6841 attttgtggc gacaaactga tgcgaaggga ttccttcaag cgttctgaat ttgcgccata
     6901 catggcttta agaacatggc tggattggac aacgcctgca gtacaggctg agccagtaga
     6961 gattgaaatt ccagctaaat ctagccgaag gagtaagagg tcatttttct gaccaggaaa
     7021 tccaatattg agaacataag ggagatgatg ttttcctcta ttcaagtaat actgaatgcc
     7081 ctccagctct gccagaaagg cagtttctag attttgtaca tgttgaaaat gttcttcttg
     7141 tttttctagg tcttctttta gggctgcaac catgcctacg atggcaggca gattttcagt
     7201 tcctgcacgt tttttctgtt cctgatctcc gccatgtaga taggaatcaa agtccatgct
     7261 agatgcgtag agaaaaccga ttcccttagg accatggaat ttgtgggcag aagcagtgag
     7321 aaaatcaatg cccaattctt ctgaatgaat tgggatttta ccaatagcct gaactgcatc
     7381 aacatgatag gcagcagggt gttgcttgag tatttggcca atttcagcga tgggaagtag
     7441 gtttcctgtc tcattattgg caaacatagt agaaaccaaa atcgtatcgt cacgtaaagc
     7501 cttttgaatt tgctgggctg tgatttcttg attttctggc tggataatgg ttgcttcaaa
     7561 cccaaagtgt tgaaccaagt aatcaattgt ttcaaggaca gcatggtgct cgatggcagt
     7621 tgtgatgata tgttttcctt gttcttggtg acgaagacag tagccaatga tggtagtatt
     7681 attgccttca gtcccaccag aagtgaaaaa gatatgttga ggttttgtcc ttagtaactg
     7741 ggctagttcc tgacgggctt ctcgcaagag tttgccagct tgacgaccat gaccatgaat
     7801 actagaagga tttccgtggg tttcttgcat aaccttggtc atagctgaaa tagcaactgc
     7861 tgacatagga gtcgttgcag cattgtccaa ataaatcaaa gaatcacctt atttcttttt
     7921 attgtaggca aagagtgggc tgactggttt tctttcgtga atacggacga tagcatcacc
     7981 aattaactca ctagcagtga tgtagcatac atttttagga gttttttctt ttgttgctac
     8041 tgaatcagtc acaagaattt ctttaatatt agtattgtca agaagctcag cagctccctc
     8101 gacgaagaga ccgtggctag aaacagcata aatttctgta gctccttcac gttcaacgat
     8161 tttagaagct tcagagaagg tacgtcctgt atttaaaata tcatcaatca agatagcttt
     8221 cttaccttca acatcaccaa taatataacc ttcgttacga gttgcatcgt cttgagggta
     8281 gtcgataatg gcgataggag catcaagata ttcagccagg ctacgcgcac gtttgacacc
     8341 tgaattttta gggctaacga caacaacatc tgaaccaagc aatcctttat cgcagtaatg
     8401 ttttgcgaat aggggaacag tgaaaagatt atccactgga atatcaaaga aaccttgaac
     8461 ctgaacggca tgcaaatcaa gagtcaggat acgatcaact ccagccttaa ccagcatatt
     8521 ggcaactagt tttgctgtaa gtggctcacg aggacaagca atgcggtctt gacgtgcata
     8581 gccaaaatat ggaaggacaa cgttgatact gtgggcactt gcacgcacac aagcatcgac
     8641 catgattaac aattccatta ggtggttgtt gacagggaaa cttgttgatt ggatgatgta
     8701 aacatcataa ccacggacac tttcttcgat atttacttgg atttctccgt ctgaaaattg
     8761 acgtgatgat agttttccaa gtgggacacc aacagcttgt gcaatttttt gtgcaatctc
     8821 ttggttagag ttgagtgcga aaagtttcat gttttttcta tctgacatta tagaccgtcc
     8881 tctgtaaact ttataaatcc tagttatatt taccttacat atatgaactg ggatttgtgt
     8941 atttttatct tttctatttt accaaaaaat ggagattatt tcagctattt ttcatacttt
     9001 tgacaaatcg aaccaatttt gaaggagctt tttggtagga aatctgattt ttctctaaaa
     9061 attgtcggaa atcctgtttg ccttgctcat gattttccac ttcaagctcc aattcgtaat
     9121 ctgttatatc aaagtatcgg ctctgatcca gtgccatgag accaatagct gttttcattt
     9181 catagcgaag cgttgttaga caaccaagaa cctgccagtt cttactttgg ataccatgtt
     9241 tcgccaattc atccagtact agcccttgag gaagttcttc cttactcaga tagttctcag
     9301 catcttttag ttgcaatttt tggttgtatt ccatgtttcc aacactctgc gggactttga
     9361 gtgtcaactc agcccagtct tcaaaggttc gaatgcgcat agcgactttc ttttctcgca
     9421 gttcaaaatc aggcgtgtcg atgtagtaat ttgtttgaag aacaggagtg acacctgtga
     9481 actggtcttt tagacgattg tattcatctt ttttcaatag tgttttcaat tcaatttcta
     9541 aatgtttcat ttttcttacc tttttttatc gttgaaagcg gatttatggt ataataagca
     9601 ttgtatttat tgtatatgaa tctggagaaa aaatcaaaga tatttttgac ggataatatg
     9661 agaacaaggg agaatatatg accttagaat gggaagaatt tctagatcct tacattcaag
     9721 ctgttggtga gttaaagatt aaacttcgtg gtattcgtaa gcaatatcgt aagcaaaata
     9781 agcattctcc aattgagttt gtgaccggtc gagttaagcc aattgagagc atcaaagaaa
     9841 aaatggctcg tcgtggcatt acttatgcga ccttggaaca cgatttgcag gatattgctg
     9901 gcttacgtgt gatggttcag tttgtagatg acgtcaagga agtagtggat attttgcaca
     9961 agcgtcagga tatgcgaatc atacaggagc gagattacat tactcataga aaagcatcag
    10021 gctatcgttc ctatcatgtg gtagtagaat atacggttga taccatcaat ggagttaaga
    10081 ctattttggc agaaattcaa attcgtactt tggccatgaa tttctgggca acgatagaac
    10141 attctctcaa ctacaagtac caaggggatt tcccagatga gattaagaag cgactggaaa
    10201 ttacagctag aatcgcccat cagttggatg aagaaatggg tgaaattcgt gatgatatcc
    10261 aagaagctca ggcacttttt gatcctttga gtagaaaatt aaatgacggt gtaggaaaca
    10321 gtgacgatac agatgaagaa tacaggtaaa cgaattgatc tgatagccaa tagaaaaccg
    10381 cagagtcaaa gggttttgta tgaattgcga gatcgtttga agagaaatca gtttatactc
    10441 aatgatacca atccggatat tgtcatttcc attggcgggg atggtatgct cttgtcggcc
    10501 tttcataagt acgaaaatca gcttgagaag gtccgcttta tcggtcttca cactggacat
    10561 ttgggcttct atacagatta tcgtgatttt gagttggaca agctagtgac taatttgcag
    10621 ctagatactg gggcaagggt ttcttaccct gttctgaatg tgaaggtctt tcttgaaaat
    10681 ggtgaagtta agattttcag agcactcaac gaagccagca tccgcaggtc tgatcgaacc
    10741 atggtggcag atattgtaat aaatggtgtt ccctttgaac gttttcgtgg agacgggcta
    10801 acagtttcga caccgactgg tagtactgcc tataacaagt ctcttggcgg tgctgtttta
    10861 caccctacca ttgaagcttt gcaattaacg gaaattgcca gccttaataa tcgtgtctat
    10921 cgaacactgg gctcttccat tattgtgcct aagaaggata agattgaact tattccaaca
    10981 agaaacgatt atcatactat ttcggttgac aatagcgttt attctttccg taatattgag
    11041 cgtattgagt atcaaatcgc ccatcataag attcactttg tcgcgactcc tagccatacc
    11101 agtttctgga accgtgttaa ggatgccttt atcggtgagg tggatgaatg aggtttgaat
    11161 ttatcgcaga tgaacatgtc aaggttaaga ccttcttaaa aaagcacgag gtttctaagg
    11221 gattgctggc caagattaag tttcgaggtg gagctattct ggtcaataat caaccgcaaa
    11281 atgcaacgta tctattggac gttggagact acgttaccat tgacattccc gctgagaaag
    11341 gctttgaaac cttggaggct attgagcttc cattagatat tctctatgag gatgaccact
    11401 ttctagtctt gaataaaccc tatggagtgg cttctattcc tagtgttaat cactctaata
    11461 ccattgccaa ttttatcaag ggttactatg tcaagcaaaa ttatgaaaat cagcaggttc
    11521 acattgttac cagactagat agggacactt ctggcttgat gctctttgcc aagcacggtt
    11581 atgcccatgc acgattagac aagcagttgc agaagaaatc tatcgagaaa cgctactttg
    11641 ctttggttaa gggagatgga catttggaac cagaagggga aattattgct ccgattgcgc
    11701 gtgatgaaga ttccattatt accagacgag tggctaaagg cggaaagtat gcccatactt
    11761 catacaagat tgtagcttct tatggaaata ttcacttggt ctatattcac ctgcacactg
    11821 gtcgaaccca tcaaatccga gtccattttt ctcatatcgg ttttcctttg ctgggagatg
    11881 atttgtatgg tggtagtctg gaagatggta ttcaacgtca ggctctgcat tgccattacc
    11941 tatcctttta tcatccattt ttagagcaag acttgcagtt agaaagtccc ttgccggatg
    12001 atttcagtaa ccttattacc cagttatcaa ctaatactct ataaaaactg tctcagagta
    12061 taattattat cttaaaggag aaaactcatg gaagtttttg aaagtctcaa agccaacctt
    12121 gttggtaaaa atgctcgtat cgttctccct gaaggggaag agcctcgtat tcttcaagca
    12181 acaaaacgct tagtaaaaga aacagaagtg attcctgttt tgcttggaaa tcctgaaaaa
    12241 attaaaattt atcttgaaat tgaaggaatc atggatggtt atgaggtcat cgaccctcaa
    12301 cattatcctc aatttgaaga aatggtttct gccttggtgg agcgtcgcaa gggcaaaatg
    12361 actgaagaag atgtacgcaa ggttttggtt gaagatgtca actactttgg tgtgatgttg
    12421 gtttacttgg gcttggttga tggaatggtg tcaggagcga ttcactcaac agcttcaaca
    12481 gttcgcccag ctctacaaat catcaaaact cgtccaaatg taactcgtac ttcaggagcc
    12541 ttcctcatgg ttcgtggtac ggaacgttac ctatttggag actgtgccat taatatcaat
    12601 ccagatgcag aagccttggc tgaaattgcc atcaactcag caatcacagc taagatgttt
    12661 ggcatcgaac ctaaaattgc catgttgagc tattctacta aaggttcagg gtttggtgaa
    12721 agcgttgata aggtcgttga agcaactaaa attgctcacg acttgcgtcc tgaccttgaa
    12781 atcgatggtg agttgcaatt tgatgcggcc tttgttcccg aaactgcagc tctgaaagct
    12841 ccgggaagta cagtagctgg tcaagcaaat gtcttcatct tcccaggtat cgaggcagga
    12901 aatatcggtt acaagatggc tgaacgcctg ggtggctttg cggctgtagg acctgttttg
    12961 caaggtttaa acaagccagt taatgatctt tctcgtggat gtaatgcaga tgatgtttac
    13021 aagttgaccc tcatcacagc agctcaagca gttcatcaat agtgaaaact ataaagtgat
    13081 atactatgct atactgtagt tatgaaacta tgtacgaaaa gcactgccat taattcctga
    13141 gaactaaatt actgattggt gtcaaaaagg aaaacttcca agcgatgata tcctgtctat
    13201 acacgaccta tagaaatctg taatatacat atccgtaaaa cgataaattc cctttttgat
    13261 tttaaatgag tatgaaaaga gaattttttg gctctttgtc aactgtagtg ggttgaagtc
    13321 agctaagctc gagaaaggac aaattt
//
LOCUS       DBEROU010000052        12384 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.052, whole genome shotgun sequence.
ACCESSION   DBEROU010000052 DBEROU010000000
VERSION     DBEROU010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 12384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12384
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.052"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(27..1706)
                     /locus_tag="ACL8CL_001839"
     CDS             complement(27..1706)
                     /locus_tag="ACL8CL_001839"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HGS1331034.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAVKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLCLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(1708..1941)
                     /locus_tag="ACL8CL_001840"
     CDS             complement(1708..1941)
                     /locus_tag="ACL8CL_001840"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase subunit epsilon"
                     /protein_id="HGS1331035.1"
                     /translation="MIYKVFYQETKERSPRRETTRALYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            2221..2436
                     /locus_tag="ACL8CL_001841"
                     /pseudo
     CDS             2221..2436
                     /locus_tag="ACL8CL_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282487.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2759..2911)
                     /gene="cibB"
                     /locus_tag="ACL8CL_001842"
     CDS             complement(2759..2911)
                     /gene="cibB"
                     /locus_tag="ACL8CL_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HGS1331036.1"
                     /translation="MMKNLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            complement(2913..3098)
                     /gene="cibA"
                     /locus_tag="ACL8CL_001843"
     CDS             complement(2913..3098)
                     /gene="cibA"
                     /locus_tag="ACL8CL_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
                     /protein_id="HGS1331037.1"
                     /translation="MTNFDILDNQFLSLSENELSDIDGGLAPLVIFGVAVSWKAIAGG
                     TALIGSGLAAGYFLGGD"
     gene            3276..3959
                     /gene="tsaB"
                     /locus_tag="ACL8CL_001844"
     CDS             3276..3959
                     /gene="tsaB"
                     /locus_tag="ACL8CL_001844"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HGS1331038.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMASLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLAYTLNIELVGMSSLLALVPH
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMAEAHLSFEEVLEKVKGTSQVTFVGEVGPF
                     VEQIQKHLPRTDYKETLPNAANLALLAWDKEADSLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            3956..4393
                     /gene="rimI"
                     /locus_tag="ACL8CL_001845"
     CDS             3956..4393
                     /gene="rimI"
                     /locus_tag="ACL8CL_001845"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HGS1331039.1"
                     /translation="MIEIKRIQQQPDLAKAIYAVMIAVYPVSPWTLEQIQADLFQDQT
                     WYALAYDGAEVIGFLAVQETLFEAEVLQIAVKGAYQGKGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIADRKAYYHDPVEDAIIMKREIDEG"
     gene            4383..5393
                     /gene="tsaD"
                     /locus_tag="ACL8CL_001846"
     CDS             4383..5393
                     /gene="tsaD"
                     /locus_tag="ACL8CL_001846"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HGS1331040.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGEAYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVMDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEITDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(5433..6739)
                     /locus_tag="ACL8CL_001847"
                     /pseudo
     CDS             complement(5433..6739)
                     /locus_tag="ACL8CL_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(7075..7766)
                     /locus_tag="ACL8CL_001848"
                     /pseudo
     CDS             complement(7075..7766)
                     /locus_tag="ACL8CL_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001839528.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8009..8563
                     /locus_tag="ACL8CL_001849"
     CDS             8009..8563
                     /locus_tag="ACL8CL_001849"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS1331041.1"
                     /translation="MQYSCGKININIPDGYGDIKDIVFSAHIIVRYNNGHCGGIDPHI
                     IGLCKKQIRRMSLYPILIIVSRDSKVIDDYKNLDIAYVDCTQCSNNFETALHVKNILK
                     LLKIRLIHCHGYSTNYFLYMLKKLDKNGFGKVKTVITCHGWVEYNLKKKFLTYFDFWT
                     YSMGDAFICVSETMKKRLESIIKK"
     gene            8691..9152
                     /locus_tag="ACL8CL_001850"
     CDS             8691..9152
                     /locus_tag="ACL8CL_001850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HGS1331042.1"
                     /translation="MDPEKRPDRFLEFAEKLFLVREDVIFIMAGNGSMWAALKEKICH
                     LKCRDNFRLLGEIYPATIVYQISDLLYIPSDTEGIPMCVLESMSQGTPVLASNVGGLS
                     EIIEHRVDGFLFEKEDVEGVCACANFLLNDSEYLKYIGENSKSKIRKHESI"
     gene            9176..10051
                     /locus_tag="ACL8CL_001851"
     CDS             9176..10051
                     /locus_tag="ACL8CL_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HGS1331043.1"
                     /translation="MDRNIDQELVSIIIPTHNRYESLIRAVKSCLHQSYKNIEVIIID
                     DNYSNVNLRNKIIHQFGYTNHRIKLILSNEDLGATNARNIGIKNSRGKYISFLDDDDE
                     YMPDRILKLMACFKKSRMKNLALVYSYGIIIYPNGTREEEKTDFVGNPLFVQMVHNIA
                     GTSFWLCKKEVLELINGFEKIDSHQDGVVLLKLLAQGYQIDIVREFLVNYYAHSKENG
                     ITGVTQKTINADEEYYNYCRKYFNLLSFNERILVTKKYYSLNIKRLLLIGDKCKALKV
                     IKKAREEKIFNEFLF"
     gene            10138..11754
                     /locus_tag="ACL8CL_001852"
     CDS             10138..11754
                     /locus_tag="ACL8CL_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1331044.1"
                     /translation="MYTIIKSNIKKFSLLTIFIVAGQLLLIYAATINALVLNELIAMN
                     LERFLKLSIYQMIVWCGIIFLDWVVKNYQVEVIQEFNLEIRNRVATDISNSTYQEFHS
                     KSSGTYLSWLNNDVQTLNDQAFKQLFLVIKGISGTIFAVVTLNHYHWSLTVATLFSLM
                     IMLLVPKIFASKMREVSLNLTSQNEAFLKSSETILNGFDVLASLNLLYVLPKKIKEAG
                     ILLKMVIQRKTTVETLAGAISFFLNIFFQISLVFLTGYLAIKGIVKIGTIEAIGALTG
                     VIFTALGELGGQLSSIIGTKPIFLKLYSINPIESNKMNDIEPNEVNRDFPLYEAKNIC
                     YKYGDKEILKNLNFCFQRNEKYLILGESGSGKSTLLKLLNGFLRDYSGELRFCGDDIK
                     KTSYLNMVSNVLYVDQKAYLFEGTIRDNILLEENYTDEEILQSLEQVGLSVKDFPNNI
                     LDYYVGDDGRLLSGGQKQKITLARGLIRNKKIVLIDEGTSAIDRRTSLAIERKILDRE
                     DLTVIIVTHAPHPELKQYFTKIYQFPKDFI"
     gene            12102..>12384
                     /locus_tag="ACL8CL_001853"
     CDS             12102..>12384
                     /locus_tag="ACL8CL_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331045.1"
                     /translation="MSEIVEMSKKISDLNDKKEFHKKNCELRFQDSVLDNINSVPGTT
                     HLFSCLNIQTLVKIILYIFRYNSCMKIFNFRDKGLIFFNKIVNGLIQNIE"
BASE COUNT         3845 a         2035 c         2634 g         3870 t
ORIGIN      
        1 gcgaagctga cgtggtttga agagtattat tcttcatctg gtgtgaggat catcgggatg
       61 atgatcggtt cacgttcggt attttcatag aggaaggggc gaatagcgtt gacaatggca
      121 ccattgacag attgcacgct agcatcctta tttttcagtg cgatacgaat ggcattgaag
      181 aggatacgct ggctttggcg aatcaagtcg ccagactctc tcatgtagac aaagcctcgg
      241 ctgaggatgt ctggaccaga tagaatcatc tgcgatttga agtcaacagt tgcgactgcc
      301 agaacgacac cgtcttcaga tagatcgcga cgatctttga ggacagctgc gccaatttca
      361 ccgatacgat ttccatcgac atagatatct tgggcgttga aatggcctgc gatacgagct
      421 gagtcagcag taagggcaag cacatcgcca ttgctcatga taaagatatt gtccttctca
      481 acaccagtat ccactgctag tccagcgtgg actttttgca tgcggtattc accgtggaca
      541 ggcatgaagt attttggctt aatcaagcag agcatgagtt tttgctcttg ctgaccaccg
      601 tgtccagatg tatggatatt gttcacttta ccgtggataa cttcgacacc agcttcagaa
      661 atgatgttaa tcagcttgtt gacgctagta gtgtttccag ggatgggact agaagagaag
      721 ataacggtat cacctggttg taattgtact tgacggtggg ttccgttggc gatacgagag
      781 agggctgcca taggctcacc ctgactacct gtacagagga taagaacttc tcctgcagga
      841 taatctttga tttcatttgg ctcgataaag gttcccttag gagctttgat gtagccaaga
      901 tcgattccgt tgacaatggc cttttccata gaacgaccaa agaccgcaat cttgcgtcca
      961 gtcttaacag cagcttctgt tgcctgctgg agacggaaga tatttgaggc aaaggatgca
     1021 aagatgatac gtccttcaat accttggata atcttcataa tggactgacc aacgactttt
     1081 tcagagttgg taaaggttgg tacttccgca tttgtcgagt cagacaggag acagagcacg
     1141 ccttcttcac caagcgcagc catacgatgc aagtccgcag gttctccaac tggagtaaag
     1201 tcgaacttaa agtcacccgt acagacgatt ttcccttgag gagtatgaat gacaatcccc
     1261 aaaggctctg gaatagagtg agtcgttcta aagaaagttg ccttgagatt tttaaaggtc
     1321 aactcggtgt tgtggttgat ttcgtaaagt ttggcgttgc gcaagaggcc gtgttcttcg
     1381 agtttcccac ggatcaaagc caaggcaagc ggtccagcat aaatagggac atttgcttgc
     1441 ttgagtagga acggaatccc accaatgtgg tcctcgtgtc cgtgtgtgat taaaacagcc
     1501 ttgacgcggt cgatattgtc tacgatgtaa gagtagtcag gaatgacata gtcgatacca
     1561 agcaagtcat cttctgggaa tttaatccca gcatcgacga tgataatctc gtcttggtat
     1621 tcaattccgt aagtgttttt cccgatttct cctagaccac cgatggcaaa aacgccgact
     1681 tcttcaggtt taagagtgta ggccatatta gaactccgta atttcgaagg cgccagtttc
     1741 tttttcgtaa tcgagcaatt tgtcagacaa gagttcgata tattcgatat tgtactctgg
     1801 gcgattttct tcgacaagtt ggcgagcagt gatacggccc tcaagttctg agctggcatc
     1861 gatgtctagg taaagcgcgc gtgttgtttc acggcgtggg ctacgttctt ttgtttcttg
     1921 ataaaaaact ttgtaaatca tatagttcct ttctatttac aggtcagctt attccaaact
     1981 tttagcagat atttgcttga tttcattcca ttttgtgtct agattgacct tgattcgtta
     2041 caattatttc aattatacca caaaatgaaa aattagtaaa agacggaaac gagaaagtta
     2101 gggggcaatt tctttggtaa gcgtttacaa aaataaagaa aatgtattat gttgataacg
     2161 gaagtgaaat ttattgcttg tcataattcc atatagattt taagtcaagg aggttgccct
     2221 atgtataact tatttgcttt tgctatcgga gttcgtttgt tagcttttat cgtttttgtt
     2281 gcttgcgttt atggaggaaa tcttccattt gccaaactgg aacgatgaca aaccaagttc
     2341 ttgtggaaaa ttacctttgt gactctctac tcgctttttc tcctcctcgt gccctatgtc
     2401 gtttggtttg tattttactt tggattaaat gcttagatgc cctgcttatg ctggggtttt
     2461 ttgtttgggg atgtagagat agtagattta cgacacgaaa attccacccc atctatcaag
     2521 atgaggtggt ctttgcattt ttctaacatg acatgacaat ggaattttca aagcttatta
     2581 tttttatggg agtcattgtt ctgctcaaaa atagagttga agcataaaaa taaaacaata
     2641 caaacagata aatcgaatgg taaagggata gtagtaatgt tcaaatcatt gagaatgctg
     2701 agcaaactgt aagagatgaa gacaataaaa tttatccttc taaaaatgtg tttcttatct
     2761 agcatgactt accaaacttt ttacgaaggt cttttccaat ggtataacct gccataaata
     2821 aagcgatacc aacacctaca ccaaagccac ccttgatttc ttgcaattcc ttattagaaa
     2881 tttcacgata gttgttcaaa tttttcatca tattaatctc ctcctaaaaa ataaccagct
     2941 gccaaaccag aacctataag tgctgttcca cctgcaatag ccttccaaga tactgctact
     3001 ccaaagataa ccaagggagc gagaccgcca tcaatatctg ataattcatt ttcagataag
     3061 gataaaaatt gattgtcaag aatgtcaaaa tttgtcattg aactttcctc cttttttctt
     3121 gttcactttt atattcggaa aagaaaagga aaagtgagca tttttttaaa ttatttttag
     3181 ttaagtgagg gcactcttgt ctgggggcaa agaaaattcc catgtgccag cttttgtagt
     3241 ataatagtaa gcagacaaaa agataggaat gtgttatgaa agtattagct tttgatacgt
     3301 ccagcaaggc tctttctctg gctattttag aggataagca ggttcttgcc gagacgacga
     3361 ttaatattaa gaaaaatcac agtattactc ttatgcctgc catcgatttt ttgatggcaa
     3421 gtttggattg gacacccaag gatttggacc gaatcgtggt agctgaaggg ccgggtagct
     3481 atacaggctt gcgaattgcg gtagcaactg ctaagacctt agcttacacc ctgaacatcg
     3541 agttggttgg tatgtcgagt ctcttggctc tggtgcccca ccaacaagaa ggtttgtttg
     3601 tccccttgat ggatgcgcgt cgcaataatg tttatgcagg attttatgaa aatgctaaac
     3661 ctgtcatggc agaagcgcac ctatcttttg aagaggtgct agaaaaagtc aagggtacta
     3721 gtcaggtaac ctttgtcgga gaagttggcc cctttgttga gcagattcaa aaacacttgc
     3781 caaggactga ttacaaagaa acattgccca atgcagctaa tctagctctt ttggcctggg
     3841 acaaggaagc agactccttg catgattttg tgccgaatta cctcaaacga gtcgaggctg
     3901 aggaaaactg gctcaagaac cataccgagt ctggtgagtc ctacattaaa cgcctatgat
     3961 agaaatcaaa cgaatccaac agcagcctga tctagctaaa gccatctacg ctgttatgat
     4021 agctgtttac ccagtcagtc cttggacgct ggaacaaatc caagctgacc tgttccaaga
     4081 ccagacttgg tatgctctgg cttatgatgg ggcagaagtg attggctttc tagctgttca
     4141 ggagactctc tttgaagcag aagtcctgca aatcgctgtc aaaggagcct atcagggtaa
     4201 gggcattgcg tcagccttgt ttgctcaatt gccgacagat aaggagattt tcctcgaagt
     4261 cagaaagtca aatcaacgag cgcaagcatt ttacaagaaa gaaaaaatgg cggtcatcgc
     4321 tgatcgaaag gcctactacc atgacccagt cgaggacgcc attatcatga agagagaaat
     4381 agatgaagga tagatatatt ttagcatttg agacatcctg tgatgagacc agtgtcgccg
     4441 tcttgaaaaa cgacgatgag ctcttgtcca atgtcattgc tagtcaaatt gagagtcaca
     4501 aacgttttgg tggcgtagtg cccgaagtag ccagtcgtca ccatgtcgag gtcattacag
     4561 cctgtatcga ggaggcattg gcagaagcag ggattaccga agaggatgtg acagctgttg
     4621 cggttaccta cggaccaggc ttggtcggag ccttgctagt tggtttgtca gctgccaagg
     4681 cctttgcttg ggctcacgga cttccactga ttcctgttaa tcacatggct gggcacctca
     4741 tggcagctca gagtgtggag cctttggagt ttcccttgct agccctcttg gttagcggtg
     4801 ggcacacaga gttggtctat gtttcggagg caggagatta caagattgtt ggggagacac
     4861 gagacgatgc agttggggag gcttatgaca aggtcggtcg tgtcatgggc ttgacctatc
     4921 ctgcaggtcg tgagattgac gagctggctc atcaggggca ggatatttat gatttccccc
     4981 gtgccatgat taaggaagat aatctggagt tctccttctc aggtttgaaa tctgccttta
     5041 tcaatcttca tcacaatgcc gagcaaaagg gagaaagcct gtctacagaa gatttgtgtg
     5101 cttccttcca agcagcagtt atggacattc tcatggcaaa aaccaagaag gctttggaga
     5161 aatatcctgt taaaacccta gttgtggcag gtggtgtggc agccaataaa ggtctcagag
     5221 aacgcctagc agccgaaatc acagatgtca aggttatcat cccccctctg cgactctgcg
     5281 gagacaatgc aggtatgatt gcctatgcca gcgtcagcga gtggaacaaa gaaaacttcg
     5341 caggctggga cctcaatgcc aaaccaagtc ttgcctttga taccatggaa taaagagtgg
     5401 ctctttgtca agtgtagtgg gtaggcgaaa agctacaatc tggagagtac gaaattcgta
     5461 ctctcttttt tgatgttcaa agtcatcaaa atccgttttt taaaattgtt aaagttccgg
     5521 aaaccaaagg ctttgcgctt gataacctta atcaaattat tggtcgcttc aagtttagcg
     5581 ttggaataag gcagcttaag tgcgttgata atcttatctc tatcccttaa aaaagtccaa
     5641 aagaccgttt gaaaaagcgg atgaaccgtt ggcagttctt gctcaatcaa tccaaagaaa
     5701 tggtcggcat tcttctcttg aaaatgaaag agcaggagtt gatagagttc gtagtgaacc
     5761 tgtaatccct cagagtaaga aagcagctta tctcgtactt cccgatgggt taagtgcatt
     5821 cgaaacgtgg gctgatagaa tcgtttctga cagagcttga agctatcctg gtgtagtaaa
     5881 tcagtccagt ggactgattt agcccgagcc tagaaacgaa aaagcgaggg ttccaaaagc
     5941 gcttgagcgc ccgatactcc aaggattttc ggtcaaattg gttcatgatt tggattcgta
     6001 ctcggttcat agctcggctc agatgttgga caatgtggaa gcggtcaaga acaatcttcg
     6061 cctttggaaa gagttgctta gcgagccgat aataagggct gtacatgtcc atggtgatga
     6121 cctcgaccag ctcccgaacc tctctctgat agcgttggaa atggtttcga atcaccgcat
     6181 gagttcgccc gtctaaaatt gcgaggatgg atttggactc aaaatcttgg gcaatgaagc
     6241 tcattttgct ctttttgaag ctatactcat cccaactcat aacttttggc aacttggtcc
     6301 aatcggtttc aaacttaaat tccctcagtt ttcggatgac ggtggaagtt gagaccgcca
     6361 atctgtgggc aatctcagtc atagactgct tttcaaggag gagctgagcg attttttgtc
     6421 tcaccatgtt ggaaatctgg caatttttct tgacaatgga tgtttgagaa acgaccacct
     6481 taaggcaatt cttgcactga aagcggcgct ttttgagatg tagtaccgtg ggtaaaccct
     6541 gacagtcgag aagcggaatt ttagaggctt tctggaagtc gtatttgatc atcttccctt
     6601 gacaatgagg acaaggagga gcgggggaat ccaactttgc ctgaacgact agatgggttt
     6661 ggtatttcag aacagacaag attttgatat ttttgtcttt caatccgagc aaatctgtgg
     6721 tattcttaag ttgttccata tgaacccttt ctaatgagtt ggttagacgc ttttcattat
     6781 aggtcatatg gggctttttt tctacaagaa acgaccctat aattcctggg gtgggattac
     6841 ccactacaga aattatagag ccaaaacatt ccaaagtctt gtctgactct ggaatgtttt
     6901 ctgttcacag ttgttttgta actgcctaat aagtaaccgc gctataacga ggtatctacc
     6961 gagattgtca attctccagt atactgttat aaaaaacaaa actggaggat ttttttatgt
     7021 ccccccatcg tcccatcgac tgttccgtag ctgcccaata acggagtata gtcggctgaa
     7081 acaagaatag gacaaaagtg cctcaagaaa agcatcgtaa tttgacaata ttattctgag
     7141 atgtttttga tgtaagccca tattttctca atgggattat actcgggtga ataggtagga
     7201 agtggtaaca gtctatgccc tgctccttac acatgttcat tctgtgaaac cttgcattgt
     7261 ccataatgat aagggatggt gtatctaaag taggtagtaa gaatttttgg aagcaagctt
     7321 cgaaaaagtc actcgtcatc gtatctttat aggtcatcgg agccatagta tattgaaaca
     7381 aaaaagggca ttagtacttc tataacagta tcgcaatctc tggtgtgcgt tgtctcgatc
     7441 ttaattcaaa taattatatc ttcttccaga gagactaact gcctgctcaa ggctcgatca
     7501 tattcgagat aaaaataggt ctcaacccct gtctcataaa tataaatagg agtcaggtag
     7561 cttaagtgat tcaattcttt aaggaaccga tttacttttt ctgggtcttg ttcgcagtag
     7621 gtacagctct tttttagact atatcccata gcctttagag cgtaatgaat agttgttgga
     7681 ggacagttga attcagcagc tatttcagtc aaataagcat cgggatgagt ttcaatatag
     7741 atctttaatt tatctctatc aactttttca attaatagtg gaagttttct taaagttagt
     7801 gtgttgaaac gtttttatac agtgctaaaa tttgaagtgt agtgcagatg tatttgtaat
     7861 agtattatag caattctttg gagaaggtcg agacaagaga aaataggtat ctttgagata
     7921 taagcatgat ttactataaa attattgagt tattcataaa aatttctcta tcaaatatca
     7981 taatgcgtcg tttaaggaga tggttacaat gcaatatagt tgtggaaaaa taaatattaa
     8041 tatccctgat ggatatggag acattaagga tatagtgttt tcggctcata ttatagttag
     8101 gtataataat ggacattgtg gaggtataga tccgcatata attggacttt gtaaaaagca
     8161 gatcagaagg atgtctctat atcctatttt gataatcgta tctagagatt caaaggttat
     8221 tgatgattat aaaaatttag atattgccta tgttgattgt actcaatgtt caaataattt
     8281 tgaaactgcc ttacacgtta aaaatatttt aaaattatta aaaatccgac ttatacactg
     8341 tcatggctac tcaactaatt attttttata tatgttaaaa aaacttgaca agaatggatt
     8401 tggaaaggtt aagacagtga taacatgcca tggatgggtt gaatataact taaaaaagaa
     8461 attcttgact tattttgatt tttggacata ttctatggga gatgctttta tttgtgtatc
     8521 tgaaactatg aaaaaaagat tggagagtat aataaaaaaa taagaagata gttgcaataa
     8581 ataacggaat taatgtatca aatagcgatt tagatgtagt aggtgttcaa gactttaaaa
     8641 aagaattttg tattccaaat aacaaaaaaa tcatttgtta tgttgaaagg ttggatccag
     8701 aaaaaaggcc ggatagattc cttgaatttg cagaaaaatt atttttagtc agagaagatg
     8761 tgatttttat tatggctgga aatggtagta tgtgggctgc tttgaaagaa aaaatttgcc
     8821 atttgaaatg tagagataat ttcaggttac ttggtgaaat ctatccagct actatagttt
     8881 accaaatatc ggatttattg tatattcctt ctgatacaga aggaatacct atgtgtgttt
     8941 tagagtcaat gtcacaagga actccggttt tagctagtaa tgttggcggg ttaagtgaaa
     9001 ttattgaaca tagggttgat ggatttttat ttgagaagga agatgttgag ggagtgtgtg
     9061 cttgtgctaa ttttttactc aatgattctg agtatttgaa atatataggt gagaatagta
     9121 aatcaaaaat aagaaaacat gagagtatat gatgaattat tagagaagag tagtcatgga
     9181 taggaatata gatcaagaat tagtaagtat tataatacca acacataata ggtatgaatc
     9241 tctgattaga gctgtgaaaa gttgtttaca tcaaagttat aaaaatatcg aagttataat
     9301 tattgatgat aattatagta atgtaaattt aagaaataaa attatccatc aatttggcta
     9361 taccaatcat agaattaagt taattttaag taatgaagat ttaggtgcaa ctaatgccag
     9421 aaacataggt atcaaaaatt ctagaggtaa gtatatatca tttttagacg atgatgatga
     9481 atatatgcca gatcgaattt taaagttgat ggcttgtttt aaaaagagta gaatgaagaa
     9541 tttagcttta gtttatagtt atggaataat aatttatcca aatggtacac gagaagagga
     9601 gaagaccgat tttgttggaa atcccttgtt tgttcaaatg gttcacaata tagcaggtac
     9661 gtcattttgg ttgtgtaaaa aagaggtgct agaattaatt aatggttttg agaaaataga
     9721 ttcacatcag gacggtgttg ttttattaaa actacttgct caaggatacc aaattgatat
     9781 agtgcgagaa ttcttggtga attactacgc tcacagtaaa gaaaacggta tcactggagt
     9841 gacacaaaaa acaattaatg cagatgaaga atattataat tactgtagga aatattttaa
     9901 tttattgagt ttcaacgaga gaatattggt tacaaagaaa tattattctt taaacataaa
     9961 gcggttacta ttaataggag acaaatgcaa ggctttaaaa gtaatcaaga aggcaagaga
    10021 agaaaaaatt tttaacgaat ttcttttttg aaatatatgt tattatatca ttttggtttt
    10081 ttctattgta tatatgacaa ctatgttcaa ttaaaattta gaaagtgaga aactattgtg
    10141 tatactatta taaaatcaaa tataaaaaaa tttagtttat taacgatatt tattgttgct
    10201 ggtcaattat tgctaattta tgcagcaact attaatgctc tggtgttgaa tgaattaatt
    10261 gcgatgaatt tagagcggtt tttgaaattg tcaatctacc aaatgattgt ctggtgtggg
    10321 ataatattcc ttgactgggt agtgaaaaat tatcaggttg aagtgatcca agagtttaat
    10381 ctagagattc gaaatagagt tgccacagac atctctaact ctacctatca agaatttcat
    10441 agtaaatcat caggaacata tctttcgtgg ctaaataatg atgttcagac tttaaatgat
    10501 caggctttta aacaactttt tttagtaata aaaggaattt ctggtactat atttgcagtt
    10561 gtgactctta atcactatca ttggtcattg actgtagcca ccttgttttc attaatgatt
    10621 atgctacttg taccaaaaat ctttgcatcg aaaatgcgag aagttagtct aaatttaact
    10681 agccaaaatg aagctttttt aaaatctagt gagactatat tgaatggatt tgatgtgtta
    10741 gcgtccttga atcttttata tgtattgcct aagaaaatta aagaagcagg aattttatta
    10801 aagatggtta tacaaagaaa gacaactgta gaaacgttag caggcgctat tagcttcttt
    10861 ctcaatattt tttttcagat atctctcgtt tttttaacag gctatcttgc aataaaagga
    10921 atagtgaaaa ttggtactat tgaagcaata ggagcactaa caggtgttat ttttacagcg
    10981 ctaggtgaat taggaggtca attatcctct attattggta cgaagcctat ttttttaaaa
    11041 ttgtattcaa ttaatccaat tgagtcaaat aaaatgaatg atatcgaacc aaatgaggtg
    11101 aatagagatt ttccgttata tgaagcaaaa aatatttgct ataagtatgg agataaagaa
    11161 atattaaaaa acttaaattt ttgttttcaa cgtaatgaaa agtatttaat tttaggtgaa
    11221 agtggaagcg ggaaatctac attattaaaa ttattgaatg gctttttgag agattatagt
    11281 ggagaattgc gattctgtgg ggatgatata aaaaaaacct cctatttaaa tatggtttcg
    11341 aatgttctat atgtagatca aaaagcttat ttgtttgaag gtacgattag agataatatt
    11401 ttattggaag aaaattatac tgatgaagaa atactacagt ctttagaaca agttggtttg
    11461 agtgtaaaag attttcctaa taacatttta gattattatg ttggtgatga tgggagatta
    11521 ctgtcaggag ggcagaaaca aaaaattact ttagctagag gcctaattag aaataagaaa
    11581 atagtattaa ttgacgaggg aacttctgct atcgatagga gaacttcgtt agcgattgaa
    11641 cgtaagatat tagatagaga ggatttgact gtcattattg ttacccatgc tccgcatccg
    11701 gaacttaaac aatattttac taagatatat caatttccaa aggattttat ttaagaatat
    11761 aggggaggga aacatgagat tctattgtga agcttatgag gtaattactg aagagaaaaa
    11821 agtatacttt aatgatattg agttagaagt taagtattcc tctgtcgaag aattgtttat
    11881 aatatgtgag aaattattag ataagaaaaa agtatcattt tttatgtaga tgagaaaccc
    11941 ttaaggtatc tgttatttga ctatatattt ctactagttc tagctaaaaa aatatcccaa
    12001 tcttatgaac tgcaccccaa aagttagatt tattctgtct aacttttggg gtgcagttca
    12061 ttttgcatag ttttggcatt catcctagat atccatataa aatgtctgag attgttgaaa
    12121 tgtctaaaaa aatatcagat ctaaacgata aaaaagaatt tcacaaaaag aactgtgagt
    12181 tgaggtttca agatagtgtt cttgataaca taaatagtgt cccgggtaca actcatttat
    12241 ttagttgttt aaatattcaa acattagtaa aaattatttt atatattttt cgatacaaca
    12301 gttgtatgaa aatatttaat tttagagata aagggcttat cttttttaat aagattgtaa
    12361 atgggctgat acaaaatatt gagg
//
LOCUS       DBEROU010000053        11690 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.053, whole genome shotgun sequence.
ACCESSION   DBEROU010000053 DBEROU010000000
VERSION     DBEROU010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11690
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.053"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(81..542)
                     /locus_tag="ACL8CL_001854"
     CDS             complement(81..542)
                     /locus_tag="ACL8CL_001854"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HGS1331046.1"
                     /translation="MGKERREQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPVLYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(509..652)
                     /locus_tag="ACL8CL_001855"
     CDS             complement(509..652)
                     /locus_tag="ACL8CL_001855"
                     /inference="COORDINATES: protein motif:HMM:NF013842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 transposase-related protein"
                     /protein_id="HGS1331047.1"
                     /translation="MAYSTDFKQGALDSIKGGHRHVEAAKVFDVGVRTLFTWEKKDVS
                     KGT"
     gene            920..1300
                     /locus_tag="ACL8CL_001856"
     CDS             920..1300
                     /locus_tag="ACL8CL_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HGS1331048.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1400..1621
                     /locus_tag="ACL8CL_001857"
     CDS             1400..1621
                     /locus_tag="ACL8CL_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HGS1331049.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1638..1952
                     /gene="trxA"
                     /locus_tag="ACL8CL_001858"
     CDS             1638..1952
                     /gene="trxA"
                     /locus_tag="ACL8CL_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HGS1331050.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2257..2790
                     /gene="queF"
                     /locus_tag="ACL8CL_001859"
     CDS             2257..2790
                     /gene="queF"
                     /locus_tag="ACL8CL_001859"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HGS1331051.1"
                     /translation="MKHQSALVVFSGGQDSTTCLFWAMQHYETVEAVTFAYGQRHHLE
                     IQITREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            2802..2975
                     /locus_tag="ACL8CL_001860"
     CDS             2802..2975
                     /locus_tag="ACL8CL_001860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331052.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVADRTDEVSNHTYGKVKLTWFEEI
                     FEEYHTKKPCSSR"
     gene            complement(3000..3668)
                     /locus_tag="ACL8CL_001861"
     CDS             complement(3000..3668)
                     /locus_tag="ACL8CL_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGS1331053.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHWNPTVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLAN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(3779..4570)
                     /locus_tag="ACL8CL_001862"
     CDS             complement(3779..4570)
                     /locus_tag="ACL8CL_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HGS1331054.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLVFTSF
                     ARGIQEELFTQEQFHFIAEVVLADGGLDKEIDKVGLSTLERSFRALIYANLLSADANQ
                     QSVFYQELNAGFRNVLLNQGLHYLSKEKDTTGFSSQYGWVHAFAHGADLLTEVVCHPD
                     FPKNRVHEVFDILGQLFKRMSIRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVD
                     FPIEEREDFYKFSNFRSCLVEVYVQLDQRNSLQDELKEAIQSFQY"
     gene            complement(4816..6612)
                     /gene="pepF"
                     /locus_tag="ACL8CL_001863"
     CDS             complement(4816..6612)
                     /gene="pepF"
                     /locus_tag="ACL8CL_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HGS1331055.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHTFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVAADEEVLDRLGKLPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRAMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDATLTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKHSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIISYSAELGE"
     gene            complement(6623..7366)
                     /locus_tag="ACL8CL_001864"
     CDS             complement(6623..7366)
                     /locus_tag="ACL8CL_001864"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HGS1331056.1"
                     /translation="MQQYFVKGSAVSPVTIEDRETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(7368..8318)
                     /gene="prmA"
                     /locus_tag="ACL8CL_001865"
     CDS             complement(7368..8318)
                     /gene="prmA"
                     /locus_tag="ACL8CL_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HGS1331057.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYVGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGELKIGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPFWTDYEATAGEMIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNTG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLTEDAYRLVKDEGYLIMSGIIKDKW
                     DMVRQSAESAGFFLETHMIQGEWNACVFKKTKDISGVIGG"
     gene            complement(8454..8882)
                     /locus_tag="ACL8CL_001866"
     CDS             complement(8454..8882)
                     /locus_tag="ACL8CL_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS1331058.1"
                     /translation="MELEISDFLGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKIYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(8863..9951)
                     /locus_tag="ACL8CL_001867"
     CDS             complement(8863..9951)
                     /locus_tag="ACL8CL_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HGS1331059.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLVPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERTTTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNF"
     gene            complement(9970..10440)
                     /locus_tag="ACL8CL_001868"
     CDS             complement(9970..10440)
                     /locus_tag="ACL8CL_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HGS1331060.1"
                     /translation="MATYGFLDILEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     ALFLKDTAEVGLDVLMDFLEDPEAEEFVMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(10731..11183)
                     /locus_tag="ACL8CL_001869"
     CDS             complement(10731..11183)
                     /locus_tag="ACL8CL_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HGS1331061.1"
                     /translation="MLVTYPALFYYDDTDGTEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(11220..11399)
                     /locus_tag="ACL8CL_001870"
                     /pseudo
     CDS             complement(11220..11399)
                     /locus_tag="ACL8CL_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
BASE COUNT         3487 a         2718 c         2119 g         3366 t
ORIGIN      
        1 gttttttata aagacacgca aatgagtata aatcagctaa tttttggttt acattcctat
       61 ttttatgcat aattattctg tcattctcta atttgtgaat caatatatct gattagaaaa
      121 ctaccattga gtttttgctc cacctcatta gtccgctcaa aacgccgtcc gctaatcttg
      181 tcatagactt tctcccctct aggagcccct gcataaggac gatagaggta gcggttgatt
      241 cccgtttcgt caatatatag gactggtaaa tcttttaggt tatccaaaat atcaagaaac
      301 tcagaaactt tctcaggctt ttgttcccta aaactggtcg tcttttttaa aatgacctta
      361 atctgcttta aaactgccca tacggagggc aaagcacaat caaaacgggc cgcaatttcc
      421 cgtaaaaaag cgtctggatg agcctctaca aaggctttca attcttctaa agggatctta
      481 cgctttttga cgactcgctt tttccgctct aagttccctt gctcacgtct ttcttttccc
      541 acgtgaagag agttctgacg ccgacatcaa aaactttggc cgcctcgaca tgtctgtgcc
      601 ctcctttgat ggaatctaat gctccttgtt taaaatcagt actatatgcc atagctttat
      661 tagaccatgc ttattttaaa ctaagcaact atagatagag aaatgaaata aaatctgaat
      721 tcgaatgtga taaggagtta gaaattgaaa caataggatt cccccattat tcacacaaaa
      781 taaaaccact aaaaaacaga gcttgccagc attcttgaaa agtagtataa ttattgcatg
      841 cgcaatcatc ttgtgacgca gagactgccc gttaatggac tttcttctat attatgactc
      901 taaaaagaaa ggagacacta tgacctattt ggaaaaatgg tttgacttca atcggcgtca
      961 gaaagaaatt gaaagtctct tggaagagac cattgcccaa cagagcgagc aaagtctgac
     1021 cttgaaagag ttctacctgc tctactatct ggacttggct gaagaaaaat ctctacgcca
     1081 gattgacctg ccagataagc ttcatctgag cccgagcgca gtttctagga tggtggctcg
     1141 cttggaagct aaaaattgcg gtctactcag tcgcatgtgt tgccatcaag atagacgctc
     1201 tagttttatc tgcctgacaa atgatgggca aaagacactg gcctcgctac aaaaaactgt
     1261 cgaagaaagc ttggaagcag gtttggattt cttgatttaa gatgattttt aaaaaaagtt
     1321 gcgtgcgcaa tcatttttct tgacattctc ttttataagg agtaaaataa agttagcatt
     1381 aaacaaagga gttttaaata tgattgaaat tacctatata gatgccagca agaacgaaag
     1441 aactgtgact ttcgagtctt atgaagactt tgaacgttcg caacaagctt gccttatcgg
     1501 cgtcgcagac tactaccctg tccaaaaatt aacttacaag ggtcataatt tggactacca
     1561 tgggacttat ggagatatct tcttctatct catgaaacaa gatttaagcc aatataacta
     1621 aaaaaaggag aaatacaatg gcaaaagcaa ttacagatgc aacattcgaa caagaaacaa
     1681 aagatggttt ggtcttagta gacttctggg caacttggtg tggtccatgt cgtatgcaag
     1741 gtccaatctt ggacaaattg tctgaagaac tttcagaaga tgtcttgaaa atcgttaaaa
     1801 tggacgttga tgaaaatcca aacacagctc gtgcttttgg aatcatgtct attccaactc
     1861 ttctcttcaa aaaagacggc caagttgtca aacaagttgc aggtgttcac acagcagaac
     1921 aaatcaaggc catcattgct gaattgagct aatcaaacga gagaccaagt actttctttg
     1981 gtctcttttt tcttgccctt tgccattttt caaaaaatat gctagactat agtagattga
     2041 aactagaata gtacacctct acttctaaaa cattgttaga aatcgatttg actgtcctga
     2101 tcgatttgtc ctgttcttat ttcattttac tatataagta gaatattacg atgtttggga
     2161 tatgccatcg ctaaaaaaac tcgatttggt attttttagc tccctaatgg gagctttttg
     2221 cgtgctctga acatttccca ttttggaagg agtactatga aacatcaatc agccttggtc
     2281 gtctttagtg gtggtcaaga ttcaacaacc tgccttttct gggccatgca acactatgaa
     2341 acagtcgaag ctgtcacctt tgcctacggc caacgtcatc acctcgaaat tcaaattact
     2401 agagaaatcg ctaaggaaca gggcattcgt caccatatcc tcgatatgtc tctgctggga
     2461 caaatcactg ctcagccaga ctttgcgacg attcatattt cctacattcc tgacaagctc
     2521 tgtgtcgagt caaaatccct caaactatat ctatttagct accgaaacca cggagatttc
     2581 cacgaaaact gtatcaacac catcgggaaa gacttggtca acttgctaga ccctcgctat
     2641 ttagaagtct ggggaaaatt cactccgcgc ggtggcattt caatcgaccc ctactacaac
     2701 tacggtaagc aaggaactaa gtatgagggc ttggcagaac aacgcctctt ccaacacgac
     2761 ctttatccag agaaaattga caaccgctaa agtcatactc aatgaaaatc aaagagcaaa
     2821 ctaggaagtt agccgcaggt tgctcaaaac actgttttga ggttgcagat agaactgacg
     2881 aagtcagtaa ccatacctac ggcaaggtga agctgacgtg gtttgaagag attttcgaag
     2941 agtatcatac gaaaaagccc tgttcctcaa gatgaggagc aaggcttttt gagtttcaat
     3001 tattcttctg ttccaaggaa attttttgca acaagggctg caagaactcc accagcgatt
     3061 ggtgcaagga tgaaaatcca aacttgttga agggctgcgc cgcctaccaa gacagctggt
     3121 gccaagctac gagctgggtt tactgaaagt ccagtaatct tcaatccgac aagaatcatc
     3181 gccatcaatg acaaaccgat taccaaacca gcaatcgcgc cattgccctt gctttctgaa
     3241 gtcacagtca tgataaccaa tacaaacaag aaagttgcga tgacttcaaa caagaaacca
     3301 ccaaagacag tgacaccgtt tgccaaggca ttttcaccaa gactagcagt tgacatacct
     3361 gagttagcca agaggaagaa gacagcgcca gaagcgatga aagctccaac aacctgacca
     3421 aggatgtagt ttacaagttc tgaagatgac aaacgtttgt ttacaaacat agcaatcgaa
     3481 acagtcgggt tccagtgagc acctgaaaca gttccgattg agtaggctgc caccacgatt
     3541 gccaaaccaa aggcaaaggc gattccaagg tgaccaaggc catcaagacc atttccaaaa
     3601 acaacagctc ctgtcccaac gaacacaagc atgaacgtac cgattaactc agcaacaaat
     3661 tttttcattt tctttctcct tttttcaaaa actagatact agtctatcaa aagtaggaaa
     3721 gggtttcaag aaaattgatt ggaaattttt tgaaaatcat agaactatta gctaatctct
     3781 agtattgaaa agactggata gcttctttca actcatcttg taaactattt ctctggtcaa
     3841 gttggacata gacttccacc agacaggatc taaagttgga aaatttgtaa aaatcctccc
     3901 tttcttctat cggaaaatca acagttttta tccaagaagc tacttgttct tgctccaact
     3961 tcccttgtaa aataggttca tagatcactc ttgctaaacg ccaatcctca tcatctgtaa
     4021 agcgaatcga cattctttta aatagttggc caagtatatc aaatacttca tgaactctgt
     4081 ttttaggaaa gtctggatga caaaccacct ctgtcagtaa atcggctcca tgtgcaaaag
     4141 cgtgaaccca accatactga cttgagaaac ctgttgtatc cttttctttt gaaagatagt
     4201 gcaagccttg atttaaaagg acattacgaa atcctgcatt taattcctga taaaaaaccg
     4261 attgctggtt ggcatctgca gacaagagat ttgcataaat aagtgcccta aaagaacgtt
     4321 caagtgttga caagcctacc ttatcaatct ctttatctaa ccctccatca gctaagacca
     4381 cctcagcaat gaaatgaaat tgctcctgtg taaatagctc ttcctgaatc cctctagcaa
     4441 agcttgtaaa aacaaggtca tcacgaattt ctggagaagg atttcccaaa tgatcaaaca
     4501 accactggat ttcttcctgg ttataatttg gtttttcttc tgctattttt cttagtaaat
     4561 cttgatacat ggtcaatacc tctacatttc tagcaactgt tcaaaaaggc agtcttaaat
     4621 gactcaatat tgaattctca attaaataca atctgatata aaatgacgta aataactatc
     4681 aataccagtt ctacagtaag ttcaaattta acatcacgac cttcaacgac atttttgaaa
     4741 atagctacaa ctaagacaaa tagaatgacg cttaacaagc ccataaacat cattctaaaa
     4801 aatttttcta ttcccctact ctcccaactc agcactatag gagataatct ggtcaactgt
     4861 gtcagacaag aattggatgg tatcacggag tggtttgtct gttgaaatat cagcaccgat
     4921 aatcatggct gactcaagtg gtgtcttgct accacctgat ttgaggagat tgagccagtc
     4981 ttcagctcca gtttcagaat gttttagatg aaggtaacca gcagtcgaga taactagtcc
     5041 tgctgagtaa gtgtaactat acaagcccat atagtagtga gcttggcgca tccaagtcag
     5101 agttgcatca tcgtcaattt caatagcatc tccccagaaa tccgtcaaaa cttccttcat
     5161 aatgctgttg agcttgcttg ctccaaaggt ctccccttct tcaatcaatg tatacacctt
     5221 acgctggaag gcggcttcca agagatgggt gataaagtta tggaagtagg tgtctgtcaa
     5281 gcgatgagcc agagcgaagc gtttttgacg tgggtcatta gactggttct ccaagtaatc
     5341 actgagtagc aattcattga aggttgacgg tgcttcaaca tagtaggtcg acatatgggc
     5401 attgaagtaa ctttgatgat tgtctgaaaa gatgaattga ccagaatgcc cgatttcatg
     5461 aatcaaggta tagacatcgc tcaaacggcc tgtccagctc atgagtacat aagggtgtac
     5521 gcgatatggg tccgccgcat aaccaccgga atccttgcca ctgttagcag caaagtccac
     5581 ccagcgctct tcttggtaac gagcaacttc ctgacaatat tcttgcccca aaggttctac
     5641 cgacttcatg accaaatcat aggcatcgtc aatagtcact tcaggattca gggcgctgtc
     5701 caagtccaat ttccagtctg caaaggtcat cttttcaaga ccatttacct tggcaacatg
     5761 cttgaggtat ctctgagcga ctggtgcaaa gtccttcatg atgaggtcaa tctggcggtc
     5821 aaacatggca cggtccactt cttgttcagc tagaagatag tcaaagacag agtcgtatcc
     5881 cttcatatca gccaagagtt tttcagactt gacctgagcc agataggctg ctgcagccgt
     5941 attttggtgc ttacgaagtc cctctgagaa ggaacggaag gatttctcac gaacctcagc
     6001 atcctcatgg ttttggtaga aattctcata ggtcacaaag ctgtttttgt aggtcttgcc
     6061 atgggcttca aagtcagcca tttcaaaatc cccagctcgc atcttagtat aaatgtcctg
     6121 cggactgtag aaaacttcac cgagatttgt caaggccttc tccacatctg cccctaagta
     6181 gtgggctttt ttgattttag cctgacgaat ggcagctgtt aaatgtggca atttacccaa
     6241 acggtccaag acttcctcat ctgctgccac caaggcatcg tcaaagaagg tcaaggctac
     6301 gctggcatct gtttcaaatt ccatcccagc ttgggcaata ttggcaaatt cgtcattgct
     6361 atagtccgtc gtctgaggca taaaaccata gttgccaata tggctcatct gaatgtagat
     6421 ctgttccaat tccgcaaagg ccttctcgaa atcctcaaaa gtgtgaagat tgcccttgta
     6481 atcacggcta aactggttga tgtcttcgcg agctttctcg attgcacgca agaaatcctc
     6541 acggtcttgg tatagggctg ttaagtccca gagttccttc tctggaaatt ctgaacggtg
     6601 tttttgttcc attttcttcc tcttatttct ctaattctac taaaacacta agggctgata
     6661 aagcgtaaag cggtgctgtt tctgctcgca aaatacgagg acctaggcct gccaaaacgg
     6721 ctcctttagc ttcaaaactt tcgatttctg caggtgagag accgccttct ggaccaaaga
     6781 taaagagcag tttggctcct gtttcaagac cagtgactgc ttgcagaagc gcagcggctt
     6841 ctccttcttt agctgattct tcataggcta ctatgataga gtcaaactgg tccagctgag
     6901 ctagaaaatc tgcttttttc tcgaaaagtt taatacttgg tacaatatta cgcttgcttt
     6961 gctcggctgc tccaagggca attttttcta gtttttcaac ttttttaccc aatttcttgc
     7021 catcccactt ggcaactgac cagtctgcag gaaaggccca gatttggcta gcccccagtt
     7081 cggttacttt ttgagcgatg aactccagct tgtctccctt gggaaatcca gatgcgatgg
     7141 tcacttggac tggtagttcc acattgtcat ttaattcttg gaccaactca aactgacgat
     7201 tttccatatc cagcacgcgc gccaagcgct tgatgccatc atcaaagact aaggtaacct
     7261 catcctcttc tttcaagcgc ataacctgaa acatatgctt gctggtttcc ctgtcctcga
     7321 tggtgacagg agagactgca ctccccttga caaaatactg ttgcatgcta gcctccaatc
     7381 acacctgaaa tatccttggt cttcttgaag acgcaggcat tccattcccc ttgaatcata
     7441 tgggtttcaa ggaaaaatcc agctgactca gccgactggc gcaccatgtc ccacttgtcc
     7501 ttgataatgc cactcatgat gaggtagcct tcatccttta ccaagcgata agcatcctcc
     7561 gtcagatgaa tgagaatatc cgccaagata ttagccacga tgacatctgc ttcaatctca
     7621 actcccttaa gcaagtctcc tgctgctaca tggatatttt ccataccagt gttgagctca
     7681 atattttcct gagcgacacg aaccgccaca tcatccaagt cataggcgaa aatctccttg
     7741 gcacctagga gtgagcttgc aatagaaaga acccctgaac cagtccctac atctagcact
     7801 gtttcgccac cacgaagaac ctgttccaag gcaaagaggc tcatcttggt tgttgggtgg
     7861 gtccctgtcc caaaggccat accaggatcc agcttgataa tcatttcccc cgcagtcgcc
     7921 tcatagtctg tccaaaaggg aacgatggtc aaatcatgag tgatacgagc tggctcatag
     7981 tatttcttcc agttgtctgc ccagtcttcc tcagccaagg cagtcgtacc tatttttaac
     8041 tctcccaaat ccataaaatc tgtcaattct gctagacgag cctgcaaatc cgcctcaacc
     8101 actgtcacat ccaccgtgtc agggtagtag gctgtcacta cgatttcttc ttgctgctcc
     8161 acctctggga aaatctctcc aaagcggtcc acatttccca catagtccat actgtcttcg
     8221 attgcgactc cttgcgctcc cagctcaatc aagagattgg aaaccaactc ctctccctca
     8281 cgcttcactg taacttttaa ctcttgccat gtttccatta ttaataccaa gcccgtaaaa
     8341 cacaaaacca aaataggaaa ttctctgaag acgcttgtgt ctaagagaag tttatctttt
     8401 tggcacagtg tttagggcgg gttcagttta gaaatgtaac tgaaccatcc tttctaatca
     8461 cttactttta aataatcttt taatctctct tgcaactgag gcacaacttg actggaacta
     8521 agaaattcct caacattcat cagctgatag ccctgtcctt catctccaaa tacgatactg
     8581 tcaaactggt tctgtgtcaa ctgaccaact agaaaaacgg actgtttgtc tgcaaaaagc
     8641 atacttgggt aaatcttact ccaaagcaga caatcttcag tcagatgaat gcccagttct
     8701 tcataaactt cacgcgctac acactcaaaa ggactttcat caccttcacg tccaccacct
     8761 ggcagttccc acatattggc ccagggaatg cttgccttat catcgcgtaa gatagtcaaa
     8821 agcttatccc cacaaaacaa agcaatctta cagcctagga aatcagaaat ttctagttcc
     8881 atcttcagtt ccttctaaca tttccttttc cagctctgct aacaagtttt cataatagcg
     8941 tttctcgtcc ctcaacattc gactactatc cattttctgt ctagcaatct tgagagcctt
     9001 acgagttcga tctacatctt tcttcacctt taattgatac caggcttgta tcacttgaag
     9061 attggacagt ttgagagaca gaaacgattt gacctgtcga atactagcat attgctccgc
     9121 ttgctcaaaa tctccttcca acaaggcgat atgaagcagg gatagttggg caactgtctg
     9181 catcatcgga gtagttgtcc tctcaagtaa tgcttgaaac tgctgtttag ctacttcttc
     9241 cttcccttcc aaaatggaaa cttcaccttg catacctaat acaccatccg caaaactccc
     9301 tcgtgcatcc tcaggaactg cttgaacaaa gtctttcaaa tcatattctt gaggaaccag
     9361 caaagtttga gcagaatgtc tcaataccag gtaggcgtat ttggtatttt cagggtgttg
     9421 tagtaattcc caaatttttg ctccatcggt gatgtcgact ggcaaaatgt tatttaggaa
     9481 gaaagataaa ttaagaaaaa tccaagtccc tgcaaaatac cagcttcttg tcaaaaatcc
     9541 aaacactatc gccaataata tcaagccgag atgaaccatc aagcctcctg aaagcatcag
     9601 gatgattctt tgatcgcttt catcctcttt taaaccaatg tattgagcac caacattttt
     9661 cagaatggct gttctactaa gatgaaacct gcctgacttt ttggtcaaaa taaaatgtcc
     9721 taatccaaaa gccaccagcc gatagcctgt caagtagcca caaaaagcat gacccagctc
     9781 atgaagaata aagattaaat acatgcttag aagagcgaag gcataaccaa aagtaaaggc
     9841 taaaactgcg gaatacccca actctgcaaa tgcgattgtt ccacaagcaa aagctagcat
     9901 aataaagaca acagctagca cataaaccaa ataagtccca attttcttca taacacctcc
     9961 aaccaactcc tagtatcttg gataagggta aaattctccc tcttccaagc caacttttcc
    10021 ttcttcaaag acttcttggt tccattccat gacaaattcc tctgcttctg ggtcttccaa
    10081 aaagtccatg aggacatcta gcccaacctc agcagtatct ttaaggaaaa gcgcaaaata
    10141 agctaaaaat tcacgggaaa atcctttttt aggcaggtaa ggaataacag tcaaatagtc
    10201 ttcctcattg actgttgact tggcaggatt gtagaaaagg accgcttcct caaaaagaat
    10261 gtcatctgat gaaacctctc cgtcttcatc caccatctcc acacctgcag cattttgcgc
    10321 ttccaataga aaactcactt ctaccgcatg gttgcgtttg tcccaactaa tctcaaagtc
    10381 aaagggaaag ttcttgtcca actcttcctc taaaatatct aaaaatccgt atgttgccat
    10441 tttgtcctct ttctatgcga ctctttaatc gccccgattg ctcggaaata tgctaaaata
    10501 gatactacca tcttaccaca aaattatttt atgtcctaat tataccatat tacctcattt
    10561 aaacccttgg tatcagtgat tttcttaaaa gtctgatttc ttcatttctc ataaaaatca
    10621 atataaaaag ccctcgaaag ggctaataaa tctataaaat caataggcga gtaactagca
    10681 caagtggacg tgctttttta ttgactatta ccacgatacc acgcttaatc ttaggcttga
    10741 actttcttat ctgcaatagc gtctgtcaaa gtctgagaaa agttaagtcc catttctcgt
    10801 cccaacttat ctgcccattt tggtatggtc aaagtctttt taatgggttc ctgacttcct
    10861 aggtattctg atacatcaac agataccata gaaataaaag atttatcaag gtcataggtt
    10921 gacacgaaat cttcatcatc tttaaaagga tcattatcaa ttaaagacaa gctattgata
    10981 tctgatggct gaggtaactc tccatcactc tctatcaaat ctgcaacagt tatccctagc
    11041 cactccgacc ccatagccaa agcctcagaa atcccctctc cttgtgtagc tgagtattca
    11101 aaatctggga aatggacaaa ataagtcgct tctgttccgt ctgtgtcgtc ataataaaat
    11161 aaagctggat acgtaactaa catttcacta cctccatatc aaaaagcagg ggctgaattt
    11221 tacaacccag cttgctttct tatccctctt tcagtgtact tattcagctt accatgaagg
    11281 attgtgatag gtctttcccc ttacttttcc attttaatat gggagccttt accgcctcta
    11341 gtctttatcc aaccatgggc cgtaaggagt ttaaccatct ctttttgtgt cataggcata
    11401 gcgcttttac ctcctgacaa caccattata acacgtgtta cacgtattgt aaaggagtga
    11461 tacttattat tctattatac ataaaagccc ctagatgtgg ttctaaggga agccaattta
    11521 ttcataccta tttttctaat gagtagtaaa aactgcttct ttatcgagca attcatcatc
    11581 tgtatagtca attgtaaaag tatctcgatc taagacagat tgaggcggag ttgaatgaat
    11641 cataggaaca ctgcgtactc tatatttttt atctccaatt tttacaaact
//
LOCUS       DBEROU010000054        11108 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.054, whole genome shotgun sequence.
ACCESSION   DBEROU010000054 DBEROU010000000
VERSION     DBEROU010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.054"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(44..1180)
                     /gene="dnaJ"
                     /locus_tag="ACL8CL_001871"
     CDS             complement(44..1180)
                     /gene="dnaJ"
                     /locus_tag="ACL8CL_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HGS1331062.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGDGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGQGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
     gene            complement(1582..1938)
                     /locus_tag="ACL8CL_001872"
     CDS             complement(1582..1938)
                     /locus_tag="ACL8CL_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HGS1331063.1"
                     /translation="MSVLDDEYLKNTRKVYNDFCNQADNYEAAKGFIDNIPVECLTIY
                     REIILAEHDSCVKNDEAVRNFVTSVLLSAFVSAMVSAMISLEIQTYKFVIPFIIGMIW
                     TVVVFLMINWNYIGKY"
     gene            complement(1940..3763)
                     /gene="dnaK"
                     /locus_tag="ACL8CL_001873"
     CDS             complement(1940..3763)
                     /gene="dnaK"
                     /locus_tag="ACL8CL_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HGS1331064.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(4243..4767)
                     /gene="grpE"
                     /locus_tag="ACL8CL_001874"
     CDS             complement(4243..4767)
                     /gene="grpE"
                     /locus_tag="ACL8CL_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HGS1331065.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(4794..5828)
                     /gene="hrcA"
                     /locus_tag="ACL8CL_001875"
     CDS             complement(4794..5828)
                     /gene="hrcA"
                     /locus_tag="ACL8CL_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HGS1331066.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKTLQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKRIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVNLLNFANLAAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(5992..6501)
                     /locus_tag="ACL8CL_001876"
     CDS             complement(5992..6501)
                     /locus_tag="ACL8CL_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
                     /protein_id="HGS1331067.1"
                     /translation="MNSRVEFRIFTIVDLDKKEEYLHEMHLKGWRYRTSRFGLFYFEQ
                     GQPDDVIYRIYDSRFLKEHKRELQDFRDRGWELIGAGSCSILRKSSSDLLPEDQVYMS
                     KGLKWEVMRSRLRSCTATFLGGLVVCMSLFREDLSMSFFLIFVLYAFLISYLIHGYFR
                     LKRKYRVDE"
     gene            7110..9443
                     /gene="hsdR"
                     /locus_tag="ACL8CL_001877"
     CDS             7110..9443
                     /gene="hsdR"
                     /locus_tag="ACL8CL_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EcoAI/FtnUII family type I restriction enzme
                     subunit R"
                     /protein_id="HGS1331068.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVMFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKEDSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYGRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGDPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            9456..10919
                     /locus_tag="ACL8CL_001878"
     CDS             9456..10919
                     /locus_tag="ACL8CL_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGS1331069.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSNM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLDPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            10919..>11108
                     /locus_tag="ACL8CL_001879"
     CDS             10919..>11108
                     /locus_tag="ACL8CL_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HGS1331070.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNDEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDD"
BASE COUNT         3380 a         2190 c         2156 g         3382 t
ORIGIN      
        1 gcaagccgac gctgacgtgg tttgaagaga ttttcaaaga gtattattct ccatcaaagg
       61 catctttaat atgatcaaag aagccttttt tctttggatt tactttcaag tcaccagcag
      121 ccgcgaattc tttcaaggct actttttggc ggtcgttcaa gcctgtcggt gttacgacat
      181 taacagtaac gtattggtca ccaactgcac cgccacgaag gctcggtgcc cccttactac
      241 gtaggcggaa tttcttacca gtctgagttc cctctggaat aaccaattca acatcaccgt
      301 gaacagttgg aatatctact gtatcaccaa gagccgcttg gacaaagttg aggttgagat
      361 tgtagaagat agtcgttcct tcacgttcaa acttgtcgct agcttccaca gaaactacta
      421 catacaagtc accataaggt ccaccgttaa agcctgcttc accttgacca gcgaggcgaa
      481 tttgttgacc tgtttccaca ccagcaggga ttttcacatg tacgctatga gcttgtttct
      541 catgacctgt tccatgacag gttgtacatg gatatttgat ttcttttcct tggccgtgac
      601 agacatcaca ggttacttgg cgacgcatca taccaagagg agtctgcgta tcgacgttaa
      661 tgacaccagc gccatgacag cgtccacaag tgactggact tgtccctggc ttagcaccag
      721 agccattaca tgtacgacag ccagcttcac gatgatactt aacttccttc tcagttccga
      781 agatagcttc ttcaaaggtc aaattgacac gatactggag atcatctcct tggcgaggag
      841 cgtttggatt gcgcgaagaa ccgcctccgc cgaagaaact tgagaaaata tcctcaaaac
      901 caccgaagcc acctgcccca ttgaaaccgc cgaaaccacc atctccacca aaaccaccat
      961 tggcgcctgc agcaccatac tggtcatagg cagcacgttt ttggtcgtca ctcaaagtct
     1021 cataggcttc ttgaacttcc ttgtacttgt cctcagcacc aggctccttg ttgatatctg
     1081 ggtgatattt tttggaaagc ttacgataag cctttttgat ttcgtctgcc gaagcgtttt
     1141 tggatacccc cagacgatca taaaattcag tattgttcat aattcaagat acaaagggcg
     1201 tcaaacggac acagccatct aggagttttg acgacggacg cttatgtcct agaaaaaact
     1261 atctttttgg cacagtccgt agcccgtgtt cagttacgaa cacccttatt cctttctatt
     1321 gatgttctga atcgaaccac catttaggtc ggattcaatt cctttctttt atatttagta
     1381 agaaattctg aaacccgtgt tcagttacga acactctttg ttcctttcta ttgatgagac
     1441 aaaacccaaa caaatgagat tccgtccctt tttatcgaaa aacagaggct gggttgcaac
     1501 ctctgcaaat aagtaaatta actttatttt gattttaaat atcctataag tacagaaata
     1561 tttatatatt tttgtctctt cttagtattt gcctatataa ttccaattga tcataagaaa
     1621 tacaactact gtccaaatca taccaattat gaacggtatt acaaatttat atgtttgtat
     1681 ttctaatgat atcatagctg ataccatcgc cgatacaaat gcagacaaca atactgaagt
     1741 aacaaaattc cttaccgctt catcattttt gacacaacta tcatgctcag ctaatataat
     1801 ttctctatat atagtcaaac attctactgg aatattatct ataaaacctt ttgctgcttc
     1861 atagttgtca gcttgattac aaaaatcatt ataaactttt cgtgtatttt ttagatactc
     1921 atcatccaat acactcatct tatttttccg taaactctcc gtctacgacg tcatcgcctg
     1981 cgtttcctgt tgcttgtgcg ccttctgctc cttcttgagc ttgttgcgct gctgcggctt
     2041 gttcgtagag tttcacagca aggccttgag ctttttcgtt caacgcttca agtttcgctt
     2101 tcatgtcgtc caagttgttg tcttcttgag ctttcttaag gtcatcaagg gcagcttggg
     2161 cagcgtcacg ttctgcgtcg aagcctttac cttcagtttc cttgattgtc ttttcagtcg
     2221 caaagattgc ttggtctact tcattacgaa ggtctacttc ttctttacgt ttcttatcag
     2281 cttcagcgtt tgcttctgca tctttcatca tgcggtcgat ttcttcgtca gtcaaacctg
     2341 agttcgattg gatgacaata gtttgttctt tttgagttcc aaggtctttg gccttaacag
     2401 acacgatacc gttcttgtcg atgtcaaatg ttacttcgat ttgaggaatt ccacgaggtg
     2461 cagctgggat atcagtcaat tggaagcgtc caagagtctt gttatctgct gccattgggc
     2521 gttcaccttg aagaacgtgg atatcaacgg ctggttggtt gtctgctgct gttgagaaga
     2581 cttgtgattt agatgttggg attgtagtgt tgcgatcgat aagttttgta aatactccac
     2641 ccattgtttc gataccaagt gacaatggcg ttacatcaag aaggacaacg tccttgacat
     2701 caccagtaat cacaccacct tggatagccg cacccatagc aactacttca tcagggttta
     2761 ctgatttgtt tggttcttta ccagtttcag ctttaacagc ttcaacaacg gcagggatac
     2821 gagttgaacc accaacaagg ataacttcgt cgatttctga caagctcaaa cctgcatctg
     2881 aaagggcttg acgaactgga acttttgtac gttcaacaag gtcacgagtc aaatcgtcaa
     2941 atttcgcacg agtcaaagtc atttccaagt gaagaggtcc agcctcacct gcagtgataa
     3001 atggcaagct gatttgtgtt gaagttacac cagaaaggtc tttcttcgct ttttcagccg
     3061 catctttcaa acgttgcatt gccatcttgt cagtagacaa gtcgataccg ttttctttct
     3121 tgaattctgc taccaagtgg tcaatgattt tttggtcaaa gtcgtcacca ccaagtttgt
     3181 tgtcccctgc agttgacaat acgtcgaaga caccgtcacc caattcaagg atagagacgt
     3241 cgaatgtacc accaccaagg tcaaatacca agattttttc ttctttgtca gtcttgtcca
     3301 aaccataagc aagagctgct gcagttggtt cgttaacaat acgttctact tcaagaccag
     3361 caattttacc agcgtctttt gttgcttgac gttgagcgtc gttgaagtaa gccggaactg
     3421 tgataacagc tttggttact ttctcaccaa ggtagtcttc agcgtagcct ttcaagtatt
     3481 gaaggatcat agctgagatt tcttgtggag tgtattcttt tccatttgca gaaacttttt
     3541 cagaagttcc catcttagat ttgatagaga taactgtatc tgggtttgta actgcttgac
     3601 gttttgcagc atcaccaacg atgatttctc cgtttttgaa tgagactaca gatggagttg
     3661 tgcggtttcc ttctgggttt gcgatgattt tgctttcagt tccttcaaga actgcaactg
     3721 ctgagtttgt tgtacctaag tcaataccga taattttaga catgtgtttt tctccttgtt
     3781 agttaatttt ctatttttct ttttgatact cttcttaagt ggcgacgccg tcagagcttg
     3841 acttcgtcaa tctctttgac taaactttga gcctaaggtc tcaaaatttg cgcaaatagc
     3901 accacggcga agagtatcgt ctttggttcg cttcgctcac tattacaaag ccaaacatat
     3961 tttatctttc ttcatagttt attgtcgttt cggacaagtt ttctattatg taaattgcga
     4021 cacgaggagt cgaaatcgat tttatttcga cgacgagtta gtaaggaagc taggcaaacg
     4081 ccatagcgat tgccgttttc tgacgagttg ctttagctac cgtcagaatt gccgaatcga
     4141 acacgcccta agctctgagt aaaaaagata gattttcttg tgttcatcga acacttcgtc
     4201 aatctcctat ttttactgcg agctttttac gagctttgta tcttagttat acaccactac
     4261 cattgctggg cgtaggatgc ggtcatggag tttgtagcct ttttgaaaga cttgggcgat
     4321 ggtatctact gggtgttcat cgtctgctgg gagagtttgg atggccatat ggtagttatg
     4381 gtcaaattcg ccatctgctg cgatttcttc aattccttct tctttcaaag cgtgaatcaa
     4441 gctttcttgc accatcgcca agcccttctt cacatcatct gtcaaacctt caactgcaag
     4501 tgcacgctca aggttgtcaa gagatggtaa gattgctttt gccaagtcct ggctacgata
     4561 acgttgcaag ttttgacgtt cttcattggc acggcgttgg atattttgca tttctgcatg
     4621 agcgcgaaga tatttgtttt cgaactcatc tgcacgttca tttgccaagt ccaactcaga
     4681 cttttcagga gttgtttctt cagctgtttc cacaacttcc tcttcttgaa cttcttctac
     4741 ttcttcattt tttatatctt gggccatgtt cgcctccttt aatgatttca atcttaatgt
     4801 acttcgtaat gattactgct gaggtagcgg taaaaatctg tcaacttcat ggtcaaaaca
     4861 cggttgacca cattgacttg attgattagc tgttggtaat ccagattaac tggaccgata
     4921 atggctagaa ttccaactcc ccgataagga atgaggaact tactactaat caccgctagg
     4981 tcagctaaac aggactcttg accgtctgca acacgaacat tttgcatctg atcctcacgc
     5041 aacccctcac gaatctccaa ggccaccttt tgcggttggt caaagaactg ataggctgct
     5101 agattggcaa aattcaagag attgaccttg cccgccatca caatgttttc gttgaacatt
     5161 tccttaaaga tgtgttcaaa gagatcgatg acattatccg ttgttgtaaa gtaacgctgg
     5221 ataatctgcg gaatctccgt ccgaatcttg tagtgaatat ctaaaacggt gtgaccgagg
     5281 aaacgttcct gaatgattct cttcagtttc agcaaatcct cctgcaagaa gttccttgga
     5341 atcagaaact gactagtaac cgttcgcgac tcgtctaggg taaataccgc caaggctgta
     5401 tgttgcccca aaacaacgat atcaaaggct gtcaaccgtt gcctgctcgg ctcaacatcc
     5461 agtgccacta ccgtacagcc actcaggtct gttagcaagt tagcagcctc ttgcagaata
     5521 tcctccaatt tgaagaattc ctgatcaaag gctttgacaa tctcatatac ctcattttca
     5581 gccagtcggt caaaatccag tgagtgtttc acatagtact gaaaaccagc aacacttggc
     5641 atccgaccgc ttgaagtatg agccttctca agcaaccctt gtttttctag ttccgccatg
     5701 tcattacgaa tggttgcact gctagagtta atagactctt gcaaggtttt tgatccgaca
     5761 ggttcgtgcg ttttggtaaa gatgtcaata atcagattta aaatatcctg ctgacgctct
     5821 gtaaccatct catcactcct ctctgtctga tcaattagca ctcaatgcat ccaagtgcta
     5881 actcatgatt ctattataca cccttagaag agaatgtcaa gacaaaaact caaaaaatta
     5941 gcactctttt atcaagagtg ctaaaaatca atctgaagac ctagaacctt gctattcatc
     6001 tactcggtat ttccttttta gtctgaaata accatgaatt agataagaaa tcagaaaagc
     6061 atataaaaca aaaataagaa agaaagacat agaaagatcc tctctaaaca aactcataca
     6121 aacaacaaga ccacctaaga aagtagctgt acaggaacga agtctagatc gcataacttc
     6181 ccatttgagc cccttactca tatagacttg atcctctgga agtaaatcag aagacgattt
     6241 acgaagaatc gaacaagaac ctgctcctat caattcccaa cctctatctc taaaatcttg
     6301 cagttcacgc ttatgctctt taagaaatct agaatcatag atacgataga taacatcgtc
     6361 cggttgacct tgctcaaaat agaacaaacc aaaacgactc gttctatacc tccaaccttt
     6421 caaatgcatc tcatgtaaat attcttcttt cttgtccaaa tcaacaatgg tgaaaatccg
     6481 aaattctact ctgctattca ttgtcttacc ccaaaattag aaaacatgcc tggcgttatt
     6541 tagttagata attctttcca cttttgactc aatctccaaa aaatataaga aatctgaatc
     6601 gcaaaaacta tcaataaaac ccaatctatt atgaaaatca aaaacacttt ccaactgaaa
     6661 gaactacctc cagtaacaaa ctttgagaaa aacggtagta gagctaaaaa gagaagtaaa
     6721 ataggaagca tccgcattgt taaaatccgt ttggcataaa aaaatcttta tttaaacgaa
     6781 aatattatag caaaatttac gccagttttt gaacggctga tgtagatatt ttatactttc
     6841 aaaatgttta aatgtgatta tttatttttg aaaaatagct caccagcccg actgaaagtg
     6901 cttatagaat gataataagt cgcctgccga aaacagcgga aaatagcggt gttatgcgga
     6961 gataatctga cgcgatgcga aagtatattg catacttatt ttcaacaatt tagcagagta
     7021 tttttataag tgtgatataa tagaagtata atttgttctg atagtttatt ttatggagaa
     7081 gtagattttt agaatgcgga gggttcaata tggttgagtt tataaagtct aagaaagaaa
     7141 tgagtgagga ggatattaaa gcaaatttca tcactcctgc tattgtatcc aaaggatgga
     7201 aaaatggtga gcatatcgct tacgaagaat acttcactga tggtcgaatt gaggttagag
     7261 gagataaggc tcgtcgtaaa gaaggaaaaa aatcagacta ttcactgtat taccaatttg
     7321 gaactcgaat tgcaattgtt gaggcaaagg ataataaaca cagcgttcga gcaggattac
     7381 aacaagctat tgaatatgga gagattttag atgttccatt tgtttattct tcgaatggtg
     7441 atggctttat tgaacacgac cgtatcacga gagaagaacg tgagctggag ttagacgaat
     7501 tccctactcg tgaagaatta ttttctcgta tgacgaagga aaaaggattg acgtacgaaa
     7561 ttacagaagc tatctcaact ccatactata cagacgcctt ctcaatgaaa acgccacgct
     7621 attatcagca aatagctatc aaccgtacta ttgaaacagt tgccagagga caaaaacgag
     7681 taatgtttgt gatggcaaca ggaacgggga aaacgttcat ggcttttcaa attattcatc
     7741 gccttcgaaa agctggtttg gctaaacgag ttttattctt agcagataga aacatcttag
     7801 tagaccaaac gatggctgaa gactttaagc cattcgaaaa ggtaatgacg aaaattacac
     7861 caaaactttt gactgctcct gaaaagttaa attcttttga aatttatcta gggctttatc
     7921 agcaactaac tggtgaagat ggaactgaaa cacattatca aaaatttgac aaagacttct
     7981 ttgatttaat cgtaattgat gaagcgcacc gtggttcagc taaggaagac agtaactggc
     8041 gtaagataat tgattatttc agttctgcga cacagattgg gatgaccgct actcctaaag
     8101 aaaccaagaa tgcttccaat acggaatact ttggtgagcc aatctatact tatagtttaa
     8161 aacagggaat cgaggatggt tttttggctc catatcgtgt tatgagggtt aatttagatg
     8221 tggatgtgga tggttatcgt ccagaaactg gaaaagttga tgctaacgga caattaatag
     8281 aagataggta ctacggcagg aaagattttg ataaaaccat tgtcattgat gatagaacgc
     8341 aaagagttgc caagtttgtt tctgattata tgaagcaaaa caatgcacga tttgataaaa
     8401 caattgtttt ttgtgttgat attgaccatg ccgagcgaat gcgtgctgca cttgtaaaag
     8461 agaatctaga cttagtccaa gaagactatc gttatgtcat gcaagtaact ggtgacaacg
     8521 ctgaaggaaa agctcaactg gataacttta tggatgtcaa ttctaatttt cccgctattg
     8581 taacaacgtc taaattatta acgacaggag ttaatgctaa aacatgtcgt ttgattgttt
     8641 tagactctaa tatccaatcc atgactgaat ttaaacaaat tattggtcgt ggcacacgtc
     8701 tttatcctca aaaggggaaa gaatttttta cgattattga ttttcgaaat gttaccaatt
     8761 tgtttgctga ccctgatttt gatggtgatc cagtgaaggt gctagaaaca ggtgcgaaaa
     8821 cagtcagtgg ttctacgccc ggtttcgtag atgaggaagg tgacccagta gaaaaatata
     8881 tcgttacaga caagcaggtt accattctta attctactgt tcaagtattg gatgaaaacg
     8941 ggaaactgat taccgaaagc ctgaccgact acactcgaaa gaatatctta ggtagctacg
     9001 ccactttgaa cgattttatc acagtttggc atacggcaga taagaagaag cttatcttag
     9061 acgaacttta taaaaaagga gtttatctag atgctattcg agagtcggag ggaatatcag
     9121 aacaagaaat cgatgatttt gatttactcc taaaacttgc ctatggtcaa aaagaattaa
     9181 ccaaaacgga acgtatcaat aaactcaaac aaagcggata tttatataaa tatagtgagg
     9241 aagcgcgtgc tgttttggaa attttactga acaaatacat ggataaaggt attggagaac
     9301 tcgaaagcat tgaaacatta aaacttccag aatttcagat atatggtgga accttcaaaa
     9361 tcatcaatac ttattttgga gataaaaaac gatatttaca agcaattaaa gaattggagc
     9421 aagagctatt tacagtagct taatgaaagg aaagtatgtc aattacatca tttgtaaaaa
     9481 gaattcaaga tatcactcga aacgatgctg gtgttaatgg tgatgctcaa cgtattgagc
     9541 aaatgtcttg gttattattc ttaaaaattt atgatagccg tgaaatggtt tgggaattag
     9601 aagaagacga gtatgagtca attatcccag aggaattaaa atggcgaaat tgggctcatg
     9661 ctcaaaatgg ggaacgggta ttgacaggcg atgaattact tgattttgtc aataacaagt
     9721 tattcaaaga gttgaaagag cttgaaataa cttcaaatat gcctattcga aaaacgattg
     9781 ttaaatcagc ttttgaagat gcgaacaact atatgaaaaa tggcgtcttg ttacgccaag
     9841 tcatcaatgt tattgatgaa gttgatttca atagccctga agatcgtcat tcgtttaatg
     9901 atatttacga aaaaattctt aaagatattc aaaatgctgg gaactcagga gaattttata
     9961 cgccacgtgc agcgactgat tttattgccg aagttcttga cccaaaactt ggagaatcaa
    10021 tggcagacct tgcttgcgga acaggaggct tcttgacttc gactctgaac cgtttaagta
    10081 gtcaacgtaa aactagtgaa gataccaaaa aatataatac agctgttttt ggtattgaaa
    10141 agaaagcatt tcctcatctt ttagcagtta caaatctgtt tcttcacgaa attgatgacc
    10201 ctaaaattgt tcatggaaat actttggaga aaaatgttcg tgaatatacg gatgatgaaa
    10261 aatttgacat tattatgatg aatccacctt ttggagggtc agaattagaa acaataaaaa
    10321 ataactttcc agcagaatta cggagttctg aaacagctga tttatttatg gctgtcatta
    10381 tgtatcgttt gaaagaaaat ggtcgtgttg gagttatttt acctgatggt tttctatttg
    10441 gtgaaggtgt aaaaactcgc ttgaaacaaa aactggtaga tgagttcaac ttgcatacga
    10501 ttattaggtt gcctcatagt gtctttgcac cgtatacagg aatccatacg aacattcttt
    10561 tctttgataa aacaaagaaa acagaagaaa cttggtttta tcgtttagat atgccagatg
    10621 gttataaaaa tttctcgaaa actaagccga tgaagtcaga acacttcaat cctgttcgtg
    10681 actggtggga aaatcgtgaa gagattctgg aaggtaagtt ctacaaatct aaatcattta
    10741 cacctagtga attggctgag ttgaattata atttagacca gtgtggtttt ccaaaagagg
    10801 aagaggaaat cttaaatccc tttgagttga ttcagaatta tcaagcggaa agagcaactt
    10861 taaatcataa gattgataat gtattagctg atattttgca gttgttggag gacaaataat
    10921 gacaccagaa caacttaaag caagtattct ccaaagagcg atggaaggga aattagtgcc
    10981 gcaaaatccc aatgacgaac ctgcaagtga attattaaag agaattaaag ctgaaaaaga
    11041 aaaacttatc agtgaaggaa aaatcaaacg agataaaaag gaaactgaga tatttcgtgg
    11101 tgatgatg
//
LOCUS       DBEROU010000055        10794 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.055, whole genome shotgun sequence.
ACCESSION   DBEROU010000055 DBEROU010000000
VERSION     DBEROU010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.055"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(153..1577)
                     /locus_tag="ACL8CL_001880"
     CDS             complement(153..1577)
                     /locus_tag="ACL8CL_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HGS1331071.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHTAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2122..4401)
                     /gene="pulA"
                     /locus_tag="ACL8CL_001881"
     CDS             complement(2122..4401)
                     /gene="pulA"
                     /locus_tag="ACL8CL_001881"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HGS1331072.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAAFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLAPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGSYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGYLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4513..6471)
                     /gene="ligA"
                     /locus_tag="ACL8CL_001882"
     CDS             complement(4513..6471)
                     /gene="ligA"
                     /locus_tag="ACL8CL_001882"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HGS1331073.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVADSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KVSQLLLQYFHSIENLSQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6564..7742)
                     /locus_tag="ACL8CL_001883"
     CDS             complement(6564..7742)
                     /locus_tag="ACL8CL_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1331074.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LNMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7822..8685)
                     /locus_tag="ACL8CL_001884"
     CDS             complement(7822..8685)
                     /locus_tag="ACL8CL_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGS1331075.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTSLYFELNWILLQGLICQRDATYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAMHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8688..10589)
                     /locus_tag="ACL8CL_001885"
     CDS             complement(8688..10589)
                     /locus_tag="ACL8CL_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HGS1331076.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFDDLRRTDKQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETISWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLATEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3249 a         2372 c         1913 g         3260 t
ORIGIN      
        1 tcaaatcgat ttctagcaat gttttagaag tagagctgta ctattctagt ttcaatatac
       61 tataatacca aaaagttcgt attttttcta taattattat tattttatag caaaaaaata
      121 aaaccaggaa aacaatttcc tggttttaca cgttatttca catcaaaaac aatggatttg
      181 acatttgtca tcgcttcaat gctatattta attccttgca ctccagctcc agaacctttg
      241 acaccaagga atgggaaatt atctggacca cgctgggttt tattattaat gtggactgta
      301 cctacttcaa gtttttcagc aatttcaaag gcttttttga aatcatttgt aaagactgat
      361 gattgaaggc cgaattcaga ttcgttggca aaggcaatag cttcctctac actagccaca
      421 cgaatgattg gtaaaacagg accaaatggc tcttcccatg ccactttcat atcttttgta
      481 acttggtcaa aaagcactgg ccagaggaga ttgccctcac gtttgattgg tgtaagagcc
      541 tgagcctctt tttcttgtgc atcctcaatc aagccccaaa tgaagtcggc tgaagcattg
      601 tcaataacag gtgtaatatc agcattgtca aatggatcac caactgttaa tttagaaact
      661 tcttcctgaa gcaaagtagc taatttatct gctacacttt cgagaacaat gacacgttta
      721 atggccgtgc aacgttgtcc tgagtagcta aaggctcccg caacaatttg cttggcagta
      781 tgttccaaat ctgcatcttc tagtacaaga gctgcatctt tcccaccaag ttccaacatg
      841 ataggacgca taccagctaa acgaccaata cgttctccaa taggagttga acctgtaaag
      901 ttgatgaaat tgacttcttt gtgctcaatg atataatccc caatttctga accacgacct
      961 gtaatggtgt tgaaaacacc tgccggaatc cctgcttctt caaatgcttt agccaacaag
     1021 agtccagaaa tggaaccttg tgttggtggc ttaaacatga ccacattccc tgcaatcaag
     1081 gcaggtgcaa ttttagaagc agataaatta actggataat taaaaggagc aatcgctagc
     1141 acgataccaa ctggttcacg acggacaaca gccagtttgt ttttacttgc tgcctcaaaa
     1201 ccaccacctt ccattgcttg tccagtgata cggagacctt cctcagcagc ataacgaatc
     1261 aagtctgctg tacgcactac ttctccaatt gctgctttaa tcccttttgc tacttctttg
     1321 gcaaggatag taccaatttt ttccttatcg cgttctaaaa tagctgctgt tttatgcaaa
     1381 taagccgcac gttcaattgc tgataaagct cgccatgctg gcagggctgc acgcgcagct
     1441 tgcatagcct catcagcttc agtctgagtc atggctggaa ctgtacccaa ttcttcttga
     1501 ttgattggtg aataaatcgt aatttcttgt tcagatgatt tccattttcc atttactaaa
     1561 ttctgatatc ttgtcaaatt cttgtcctcc tgaaaatgat taagatatat tttaccaaat
     1621 tttcagaaaa tcacaaccct ttttgcaaaa tttttgagat tattttcaca atcttgattt
     1681 ttcaaagtat actcaataaa aattaaaaaa atccactacg tcaaggcgag gctaatgtgg
     1741 tttgaagaaa ttttcgaaga gcgtgaatga gtatcatcta tagtaaaata aaaaaactga
     1801 acaatttggt tggggacagc caaaccaatt tctcacaatg tttcagaaac aagggtgtgc
     1861 tattccaatt tcagcctact ataactgtca tagattgctg aaacaaagtc tagataaaag
     1921 tcttcataat aaaaagacct cctatcaagt gttcaaaaac tttgatagga ggtcttgttt
     1981 tgtgaaaata tttatcaaat tttctataca agtgagctgt tagccaggtt ctttctattc
     2041 tttcaatttc aatgaatgga ttttttacta atactcataa ctgggaattt gtctgtgtaa
     2101 aaatagcgag atagatggta tttataaaac actcaagaca gctagactaa tatcatttaa
     2161 aacattatct tcttttgagc gactgttggt taccaacata gctaaatttc ctgcattttc
     2221 aaattgatag ggttctgatt tagcattcac aaccaccaag aggtgttctt tgccgtgaac
     2281 ttcatagata aggtagccgc tatgttcaat cgcagaatgc acaaagacat gatggtaaat
     2341 ttcatcatag ctagagtaag aaaaggcacc agtttttgtc ttcaatcgga tgacttgacg
     2401 gataaactca atactgtctt gacgctcatt aatcaagttc cagttcactt ggttcacact
     2461 gtcaggagca ttatagctat tcatcgcacg ctctctatca tcatgggtca actcaccatt
     2521 ttcaccagtc gcaaccagtt tggtacgacc aaattcttga ccgatttcca taaaggccat
     2581 cccctgcatg agcagattca tggctgtggc agtttcgacc ttgcgcatga tttgctctga
     2641 actttggtct ggatgaaggg ttgccaataa atcgtgaaga ttgtaattgt catgggcttc
     2701 tacatagtta agcacctgat ttggatgtgt atagcttcct aattcacgac ttcctaggat
     2761 tgctttagct agaattggct ctgtcgcagc accactgaca aaacctgact tgatagcacc
     2821 ataaacttct ccccctttga cagcatcgcg ctgattgtca ttaaagaaac caatatttgg
     2881 catctggtag gcattgtcct tcttggcctt atcataaggg gcaagacctg ttcccatatc
     2941 ccatccttct ccatagagga taatgttgga gtcgatttca tccaagcttt gacgaatcat
     3001 ctgcatggtc ttgacatcat gaatccccat caagtcaaaa cggaagccgt caatattata
     3061 ttcctgcacc cagtatagaa gagaatcaat catatacttg cgaaacattt cgtgttcact
     3121 ggctgtttca tttccaacac ccgttccatt ctggaaggta ccatctggat tcatacgata
     3181 atagtaatca gggactgttg tttggaatgc tgcatcaaca actgagaagg tatggttata
     3241 gactacatcc ataatgactc caatacccgc atcgtgataa gcttgaacca tcaccttcaa
     3301 atcacgaatg acctgagctg gatcatctgg attagttgaa aaactagttt ctggcgcgtt
     3361 atagttttgt ggatcataac cccagttgta ggttacattt ccatcctcat cgtattcttt
     3421 atgacggtct gcaattggtt gcaattgaac ataattgtag cccagcttct tgatgtaatc
     3481 aaaagcagtt gactggccgt attggttaac tgttccagcc tgagcagcac ccaagaaagt
     3541 tcctcgaaga tgttcatcta cacccgatgt aggtgattta gtcaaatcac gaatatgcat
     3601 ttcacagata actgccttac atggattttc caagcgccaa gtagcctccg aaccgtgctt
     3661 aacctcgaag ttttcaactt gcttttctac atggctcaga atagctgaac gtttgccatc
     3721 agggctggtc gcgattgtat aaggatcacg tgtcagtgtt tggtgatgag ggaattggac
     3781 ttgatactga taagtcttac ctaccaaatc ttcttcaaca tccaaactcc agacaccgat
     3841 tgtattgtcc ttatgattat aagagtagct attgcctctt ttcatctcaa aagtcttcca
     3901 aacgggtgca tcattagcag ctgattcata aacgacaact tgcacttctg tcgctgtagg
     3961 tgaccagagg gaaaaatgag cctgattgtc ctctacacgg caacccaatt ctccttggta
     4021 accccaatga tgatcaaaac tagcactgtt aatggcctta tcaaaggcaa aaggattttg
     4081 atttttatag aaaggactgg caatagcagg attttcagag taataaatcc tatcatcgcc
     4141 ttccaaaatc cagacctctg ttaatagggg atagtgatta aaacggatag aatattcttt
     4201 actagtttga cctgtatgaa ccacaaaatt caagctttct ataacatgtg aacttgggtg
     4261 ttcaaagcta aataaagctc caaaataatc ttctttgtag gttagcaaat caattcgttg
     4321 atcctgactt tttacaaagg agcaagtgtc atattctcca ttcttacgat ggtaatgaat
     4381 gcgcataggg tagttataca ttttttattt ttccttttta ctttgtttct atttcactaa
     4441 taaatttttg tcaatctcgt ctcaattaac agacatagtc atattctcta aactctgttt
     4501 ttaaacgatc cattacaaac tttctagcca tgcctcatct ctgacctgga taccaagttc
     4561 ttgtgctttt tgcagtttac ttccagcgtc tgcacctacc acgacgaggt cggtcttttt
     4621 agaaacacta cctgtcactt tggcacccag actttcgagt ttacttttag cttctgagcg
     4681 cttgagtcgt tccaattttc ctgtcaatac cacggtcaaa cctgacaagg ccgcatccgc
     4741 tactaccgtc tgtcctttat agtccagatt gaccccagtt tctttcaatt ctctgagcag
     4801 aatttcagag ccttctgtcg caaaataagt ctgaagactt ttggcaatca cgccacctag
     4861 actttcaata ctagccactt cctctgaatc tgcctgagac agattttcaa ttgaatggaa
     4921 atattgaagt aaaagctgac taaccttgct tccgacatga cgaattccca aaccaaataa
     4981 gagcttctcg gcagaatttt cctttgatgc ttggatagcc tgatacagtt tagcagcgga
     5041 cttttcctta accccctcta aaaggaggaa atcctcttct tgcaaacgat aaatatccgc
     5101 cacatccttg actaaattag cagcaaaaag cttctcaaca atagatggac caaggcctgt
     5161 aatattcata gcatcacgag aagcaaagtg aatcaagcct tccatgattt gagcagggca
     5221 acgcggattg atacaacgta gggccacttc atcttcaaag tgcaacaagt cagagttaca
     5281 acttggacag tttgtaggga tatctagttt ttcttcagaa acccgtttgg actctaccac
     5341 acgtaaaacg gcagggatga tgtcaccagc cttatataca atgaccgtat cgtcttttcg
     5401 gatatctttt tcagcaatat aatctacatt gtgcagggtc gcacggctaa cagtcgtacc
     5461 ggcaagttgt actggtgtta gattagcagt tggagttaca acaccggtac ggccaactgt
     5521 ccagtcaact gataagagtt gagcttcttt ttcttcggca gggaacttgt aggctactgc
     5581 ccactttgga gccttaactg taaaaccaag ttcttcttga cttgctaggt cgttgacctt
     5641 gattaccact ccatcaatat cgtaaggcag attttcccgt tcctgtccta cttcttggat
     5701 aaaattccag atttcatcta tgttttcagc caagattcgc ttaggattga ccacaaaacc
     5761 tagttgttct aggtacttca aacccttttc ttggctatca cgagttgaag ggctggcttc
     5821 ttgatagaga aacgttgcaa gattacgctt ggcaactact gctgtatcca actgacgcag
     5881 agttcctgct gccgcattac gaggattagc aaattcaggc tctccatttt cttggcgcac
     5941 ttggttaact tggtcaaagg aagcgcgtgg catgtaacat tccccacgaa ctgtgatatc
     6001 tagttcttct ggcaaagtca aagggatgtc cttaacacgc ttgaggtttt ctgtgatatt
     6061 ttcaccaatt gaaccatctc cacgtgttac cccagcaacc aaaatcccct tttcataagt
     6121 cagcgagata gataagccat cgattttcag ctcacaaata taggtcggat gagccacttc
     6181 cttacgaaca cgcgcatcaa aagcatctag ctcctcacgt gaaaaagcat cctgcaaact
     6241 ataaagagga tactgatgac tgtatttttc aaaaccatct aaaaccttgc caccaacacg
     6301 atgagtcgga ctgtctgcta gcacttgctc tggataagca gtttctaact cgaccaactc
     6361 acggtaaagg cggtcatact cactgtctgc aaccgaggga ttatcgctgg tatagtactc
     6421 agtcgcatag cgattgagca aagcgactaa ctcattcatt cttttattca taagaccatt
     6481 ttaccataaa acaagccctc ctcacaaacg agaagggcgg aaaaaacact tagtttgaaa
     6541 ttatttttga aactcaagca accttatatc aatttttcaa aatgagttcg aacataaata
     6601 aacgatatac aagacaagat gataacacca cttccaatta tcaggaaaga agagagatgt
     6661 acacttggca agactgtcat aaatcctttt gcaataggca taaatagaat agctaaggta
     6721 aaaattgtac tcagtactct tccaagaaat tcgctctcaa ccttggtttg tacttgagta
     6781 aaaaagtgaa tattaaaaat cgtcataaac aattcacaaa ctaaatttcc agaaaaggaa
     6841 agaaaagttg gaagtggtaa tcccatcata aaaactccga cacctgtcaa agccagtaaa
     6901 atcaaaagat tataaatatt agctttaatt ttactagcta gaagagcccc aatgatggaa
     6961 ccaatagccc ccatagttaa aatacttgca taggctcctt ctgacccgta aagctgattc
     7021 gaaaagggaa gtagaaattc aaaagctgca aaaaagaaat taacgctgga agctaccagc
     7081 aaaaggaaga aaatttcttg ctgatgccag atatagtgta acccatcctt gatatctaca
     7141 aaaatatctc tcccagtaaa agcctttttc tcttgaactt ttgcttcctc ttttggaagg
     7201 aaagccacta gaacaaaagc aatgaaaaaa gtcagcgagt ctagcagtag cgtcatattg
     7261 agacttgcaa actgtaaaac aaggaaggaa agaacaggag agctaacacc tacaacctgc
     7321 aaaaccagct ctaagcgaga attatagatc acaatctcat ctttctccac cacttcagtt
     7381 atgatagctt tattggctgt gcgagaaaag gcaaaagcaa tagcctgcac aatgttagca
     7441 acaatcaaag cgccaatcat ccagctatca ttccttatga aagaaatagc cagacaaaga
     7501 atcccacaaa caagatctgc cgtcattaaa atcttacgac gagaaaaacg gtctgaaata
     7561 actccgccaa agggattgac gagaatagat gtgacgagct cagaaatctg atacattcct
     7621 aaaactgtct gtcctatagt ccccatagaa gccaaccaga cactatttcc ataatcatag
     7681 agcatatttc ccattttatt gatagcccca cggctaatca actgcactgc atagcgattc
     7741 atattaaagc tcctctcaaa ttttgaaact attgtatcaa aaccgaaagg agctttttat
     7801 tttttccctt atttgggaaa attaactttt gacaaatttt tcgtagtgtt cctgataata
     7861 ggctacttgc tctggaagac ctaacacatc aaaaatatgc atggcctctt gcatctgctt
     7921 acagccttct ttacactgtc ctttttgata taaggcaaaa ccttttaaat aatggaaaac
     7981 attacgctca taaagcttaa tacctttgtc aataatcttc tctgtataag cctcaaaata
     8041 gttggcatta taaaaagaag aatgctctaa acaatgctgg taacaattga gggccaaaat
     8101 caaaactaat ctcttatggc gactaatctc ttggtaaaat tcctccctct ccataacttc
     8161 tctaccaatc cgagtgacat agtctacatc gtagaaacta tagaggttac cgaaaagaat
     8221 caactcatac atggtccatt cttctgtttt gaagagataa tctgctacct tatccaaatc
     8281 atcctgcttc atatcataag tcgcatctct ttgacaaatc agaccttgta gcaaaatcca
     8341 gttcagctca aaataaaggg aagtcgtcga actcttagac ttttcaagtt gttctctttg
     8401 aagcttttga aaacctgcaa tatcgtttga atagtaaagt gggataatct gtgccatcat
     8461 agacacatgt tcatgattat gaaaattcct tgccttatcc atgaaatttt cgattgttac
     8521 atgaatgtta tccaaaatct caaagaaacg ggagactgcc aggtcagact ccccaagctc
     8581 aaagcgagat aactgagagg tagagcagga ttcgcctgct gcttccttta aagaataatt
     8641 tccacttgtt cgaaattcac gaaatacttt tccaagatgt tccatcttta cacctgatct
     8701 gataattctt cccactcaag catagcttct tcctgacgat ggctgatttt gtccagctca
     8761 gcctgtaatt ccatgagttt gtcggcatcg tttgtttcca acatttgttc agaaatggct
     8821 tggctttgac tttctagctc ttcaatttca gcttctagac tttcgatttg tcgcatgagt
     8881 ttgcgaactt ctttttgact ttctttctgg gcctgatagt cattgactgg acttgcttcc
     8941 tttgcttgat tgctagttga agcttcctca gtctgactca tttctgctgt tgctttcttc
     9001 tcaacatagt agtcgtaatc tccaaggtag agagttgaac cattctcaga caattccaaa
     9061 acatgagttg ccacacgatt gataaagtaa cgatcatgac tgacaaaaag caaggtacca
     9121 tcaaagtcaa tcaaggcatt ttctagcact tccttactat caatatccaa gtggttggtc
     9181 ggctcatcca gaatcaaaaa gttattgttt tccatagaca atttagctaa aagcaaacga
     9241 gctttttcgc caccagatag catgccgact gattttttaa catcatctcc tgagaaaagg
     9301 aaggccccaa gacggttgcg gatttcaact tctggtgtca gtttgaaatc attccagagt
     9361 tcatccagca ccgtattact tggtgtcagc ttgctttggg tttggtcata gtaaccaacc
     9421 tcaacattag cgccaaagcg cttttcacct ttgataaaag gaatctggtc cacaatagac
     9481 ttgataaagg ttgacttgcc gataccattt ggaccaacga tagcgacagc attcatctta
     9541 cgaagatcta ggttgatagg ttgtgacaaa acttccccgt cataaccaat agcagtattt
     9601 tcaacagtca aaacaacatt gcccgacgtt ttttcagact ggaaggtcat gttggctgct
     9661 ttcttgccag cttcaggctt gtccaaacgc tccatttttt ctagttgttt acggcgagat
     9721 tgagcacgtt tggtcgttga agcacgaact agattgcgat tgacaaagtc ttccagagca
     9781 gcgatttcct tctgttgctt ttcatagttt tttgcctcag tagctagctt ttgctccttc
     9841 aactcaacaa aacgagagta attccccaca tagcgatcca aggaatgctt ggtcaaatct
     9901 agcgtaattg tcgcaacctt gtccaagaaa taacggtcgt ggctgacgat aatgagggca
     9961 ccgctatagt ttaccaagta attctctagc caggagatgg tttcaatatc caagtggtta
    10021 gttggctcgt ccaagaccaa gagattgggc ttttcaagga gcattttagc tagagccaaa
    10081 cgagtatttt gaccaccaga aagctcagca attttcatct gccacataga ctcgtcaaac
    10141 ttgaatccat tcaaaatcgc tcgaatatca gcttcatagg taaagccacc tgcttggcga
    10201 aaattttcag ataagcggtc ataatctgac atcagtttat ccaaatcctc accagacttt
    10261 tcacccatct ccagctccat ctgacgcagt tgtttatctg tccgacgtaa gtcatcaaag
    10321 acatgaagca tctcatcata gatggtattt tcagactcaa aacggctatc ttgagctagg
    10381 taagacagag aaatatcttt tttcttattg atttctccac tagttggctc ctcttctcca
    10441 actaaaatct tcaaaagagt agacttacct gcaccatttt tcccaacaag ggcaatccga
    10501 tctcgttcat caacctgcag gttgatatta tcgaaaagaa cctctcctgc aaaagaacgt
    10561 tcaattttat tagcttgtaa aataatcata caagtagtat agcatgtttc cctaaggcat
    10621 tcaagataat cgtaagtctt ttagtacaac ttttataaca taaaataaac taaattatgt
    10681 atattttata ttagattcct tcactatctt gttggatttt ctaaccagct aatcttgttt
    10741 caaatagtta tcgcacaagc ctattattta attcttttca ttatttacgt acgc
//
LOCUS       DBEROU010000056        10388 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.056, whole genome shotgun sequence.
ACCESSION   DBEROU010000056 DBEROU010000000
VERSION     DBEROU010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.056"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..357
                     /locus_tag="ACL8CL_001886"
                     /pseudo
     CDS             1..357
                     /locus_tag="ACL8CL_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            complement(485..1507)
                     /gene="cbpF"
                     /locus_tag="ACL8CL_001887"
     CDS             complement(485..1507)
                     /gene="cbpF"
                     /locus_tag="ACL8CL_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpF"
                     /protein_id="HGS1331077.1"
                     /translation="MKLLKKMMQVVLATFFFGLLGTSTVFADDSVGWQFVQENGRTYY
                     KKGDLKETYWRVIDGKYYYFDPLSGEMVVGWQYIPAPHKGVTIGPSPRIEISLRPDWF
                     YFGQDGVLQEFVGKQVLEAKTATNTNKHHGEEYDSPAEKRVYYFEDQRSYHTLKTGWI
                     YEEGHWYYLQKDGGFDSRINRLTVGELARGWVKDYPLTYDEEKLKAAPWYYLDPATGI
                     MQTGWQYLGNKWYYLHSSGAMATGWYKEGSTWYYLDAENGDMRTGWQNLGNKWYYLRS
                     SGAMATGWYQEGSTWYYLNASNGDMKTGWFQVNGNWYYAYDSGALAVNTTVGGYYLNY
                     NGEWVK"
     gene            complement(1526..2347)
                     /gene="cbpG"
                     /locus_tag="ACL8CL_001888"
     CDS             complement(1526..2347)
                     /gene="cbpG"
                     /locus_tag="ACL8CL_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpG"
                     /protein_id="HGS1331078.1"
                     /translation="MNVEGRGSANFIKDNVLITAAHNYYRHDYGKEADDIYVLPAVSP
                     SQELFGKIKVKEVRYLKEFRNLNSKNAREYDLALLILEEPIGAKLGTLGLPTSQKNLT
                     GITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSGSAVYDASHRVVG
                     VHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGWKKINGSWYYYRQHDKQTGWQE
                     INDTWYYLDSSGKMLTDWQKVNGKWYYLNSNRAMVTGSQTIDGKVYNFASSGEWI"
     gene            complement(2428..2667)
                     /locus_tag="ACL8CL_001889"
     CDS             complement(2428..2667)
                     /locus_tag="ACL8CL_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331079.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(2817..3362)
                     /locus_tag="ACL8CL_001890"
                     /pseudo
     CDS             complement(2817..3362)
                     /locus_tag="ACL8CL_001890"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3344..3585)
                     /locus_tag="ACL8CL_001891"
                     /pseudo
     CDS             complement(3344..3585)
                     /locus_tag="ACL8CL_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(3586..4218)
                     /locus_tag="ACL8CL_001892"
     CDS             complement(3586..4218)
                     /locus_tag="ACL8CL_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HGS1331080.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGHEEF"
     gene            complement(4232..5227)
                     /locus_tag="ACL8CL_001893"
     CDS             complement(4232..5227)
                     /locus_tag="ACL8CL_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HGS1331081.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSMTCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVVDQDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQTQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFQLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(5224..5922)
                     /gene="liaF"
                     /locus_tag="ACL8CL_001894"
     CDS             complement(5224..5922)
                     /gene="liaF"
                     /locus_tag="ACL8CL_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein
                     LiaF"
                     /protein_id="HGS1331082.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAVTILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(6000..7010)
                     /gene="fni"
                     /locus_tag="ACL8CL_001895"
     CDS             complement(6000..7010)
                     /gene="fni"
                     /locus_tag="ACL8CL_001895"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HGS1331083.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDDS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SSQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKAVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(6994..8001)
                     /locus_tag="ACL8CL_001896"
     CDS             complement(6994..8001)
                     /locus_tag="ACL8CL_001896"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004631 - phosphomevalonate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HGS1331084.1"
                     /translation="MIAVKTCGKLYWAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRPNPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQNLLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIEQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(7988..8941)
                     /gene="mvaD"
                     /locus_tag="ACL8CL_001897"
     CDS             complement(7988..8941)
                     /gene="mvaD"
                     /locus_tag="ACL8CL_001897"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HGS1331085.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEVVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMDFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGHRYRLIVSKTKDLSQDDCC"
     gene            complement(8923..9801)
                     /gene="mvk"
                     /locus_tag="ACL8CL_001898"
     CDS             complement(8923..9801)
                     /gene="mvk"
                     /locus_tag="ACL8CL_001898"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HGS1331086.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVP
                     AESPWRLYEEDTLSMAVYASLEYLNITEACIRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(9923..10252)
                     /locus_tag="ACL8CL_001899"
     CDS             complement(9923..10252)
                     /locus_tag="ACL8CL_001899"
                     /inference="COORDINATES: protein motif:HMM:NF024736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1331087.1"
                     /translation="MTRKVYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFRRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         3038 a         2418 c         1832 g         3100 t
ORIGIN      
        1 agtgctacac agatgtgact gaattttcca ttccagcaag tactcaaaag ctttacttat
       61 caccagtttt agatggcttt aacagcgaaa ttatcgccta taatctttca acttcaccca
      121 acttagaaca agtaaaaaca atgttggaac aggcattcac agagaagcac tatggtggag
      181 tccttctttg ggattctgaa atcggaaatg ttttatggct atgagaaaac atttaaatca
      241 cttaatcaat tggaacaagc cattatagac tatattgatt attacaacaa caagagaatt
      301 aaggtaaaac taaaaggact tagtcctgtg cagtacagaa ctaaatcctt tggataaatt
      361 atttgtctaa cttttggggg gcagtacaga aaaaccttga tactatgcgt tttttgattg
      421 tgaagacttt tatccaccta ttatacaaag ttaagtatcc atcacagttt acaattagcc
      481 ttcattactt aacccattca ccattatagt ttaagtagta accacctact gtggtattaa
      541 cagctaaagc acctgaatca taggcatagt accagttacc attgacttgg aaccagcctg
      601 ttttcatatc tccattactt gcatttagat agtaccaagt cgaaccttcc tgataccaac
      661 cagttgccat agctcctgat gaacggagat agtaccattt gttcccaagg ttttgccagc
      721 cagttctcat atcaccattt tcagcatcta gatagtacca agttgagcct tccttatacc
      781 agccagttgc catagctcct gacgaatgga ggtagtacca cttattacct agatattgcc
      841 aacctgtttg cataatgcca gttgctggat ctaggtagta ccatggagct gcttttagct
      901 tctcttcatc ataggtaaga gggtaatcct taacccaacc acgtgctagc tctcccaccg
      961 tcaatctgtt gatgcgcgaa tcaaagccac catccttctg taaataatac caatgaccct
     1021 cttcataaat ccaaccagtt tttaaagtat gataactacg ctgatcttca aaataataga
     1081 ctcgtttctc tgctgggcta tcatattctt ccccatgatg tttgttggta ttcgtagcag
     1141 tttttgcttc taaaacttgc ttgccaacaa attcttgtaa tacaccatct tgaccaaaat
     1201 aaaaccaatc tggtctaaga gaaatctcta ttcttggaga aggaccaatc gtaaccccct
     1261 tgtgtggagc aggtatatat tgccagccga caaccatctc tccggataaa ggatcaaaat
     1321 aatagtactt accatcaatc actcgccagt aggtttcttt tagatccccc tttttgtagt
     1381 aggttctacc attttcttgg acaaactgcc atcctacaga atcatctgca aatactgtac
     1441 tggtccctag caaaccgaag aaaaatgtgg ctagtacgac ttgcatcatt tttttcaaaa
     1501 gcttcatttt atatatcctc caacattaaa tccactcacc agatgaagcg aagttataaa
     1561 ctttaccatc gatagtttgg ctacctgtaa ccattgctct atttgaattg agataatacc
     1621 attttccatt tactttttgc caatctgtaa gcatcttacc ggaactgtct aaataatacc
     1681 aagtatcatt tatctcctgc caacccgttt gtttatcatg ttgtctataa tagtaccaac
     1741 taccatttat tttcttccat ccttcaagag agtaaccttt aagaaccgaa taaataaatg
     1801 gcaaatttcg ttcatttaat ttaactgcac tgttaatttg attagctcca tctcctaaag
     1861 tatgcactcc tactacgcgg tgactagcat cataaactgc agatccacta gacccctcta
     1921 aagtatcaac ttggtaatcc aagaacatgc catcatcact taaaacttgt tttttatctg
     1981 tatacatttg atgaatttta aaattatatg atagatagcc tgtgatagtc acagttattc
     2041 ctgtcaaatt tttttgacta gtaggaagac ccaaagtccc taattttgca ccaatgggct
     2101 cttctagaat taataaagcc aagtcatatt cccttgcatt cttagaattt aaatttctaa
     2161 attccttcaa ataacgaact tcctttactt tgatctttcc aaatagttct tgacttggac
     2221 taacagccgg aagaacataa atatcatccg cttctttccc atagtcatgt ctgtagtagt
     2281 tgtgagccgc tgtaattaac acattatctt taataaaatt cgcacttccc ctcccttcta
     2341 cattcattcc atcggctact ccataatact tagataaaac catagctgaa gtcgaatacg
     2401 gatactgtaa agtattatca attttaatca aatcatcatt accgataata cttctgattg
     2461 cttttggtag tatgaaccat acgttggtga aatctcagat aatgaagaat cattagactc
     2521 tggacctttt tctagtgtct cacttacctc atattcttca cctgtactag aaataacact
     2581 caaagcagat actgtcgata actggctagc caataaagta ctcgcaataa ttgaaatacc
     2641 caatttttta taaacagttt tcttcattat tgtatcctcc taatgtaatt atagcgtact
     2701 attctaaatt tcttaatcta ctatagaatc aagaaatcta ccaccttctt taaataccct
     2761 ccattatcac ataaacaggt aaacttttca attaatgact gcgcttttca atcacgctag
     2821 aggtacttac ttgcttcttt gatactaagt tcagccattc tttccttgtt tttctcaata
     2881 aagcatgtta cccaagtggg attcgttttg gagtagtctc gcagagtcca gccaatggct
     2941 ttattgataa aaaattctgt ttggttcaag ttatgaagta gaatcttttc cattaattga
     3001 gtattggtct tctcttttat taacaactgg tggtcaatag cgacacgtct cagccagata
     3061 ttatctgata ggctccattt tatactcaat gaaaatcaaa gagcaaacta ggaagctagc
     3121 tgcaggttgc tcaaaacact gttttgaggt tgcagataga gctgacgtgg tttgaagaga
     3181 ttttcgaaga gtattaagat tatttcttct agttcagggt gttcatacac caaactccct
     3241 actactcgat ctaggatatc taccgtgtcc caccaggatt ttgtcacgac taactgctct
     3301 agcttaggca aatcggtttc ctttagataa gactgcattg ctttcaaata gttagcagcc
     3361 acatattggt attttctagg ctccttttcc cagcaagtgt ctgcaaaatc ccaatcgata
     3421 atctttgttt ttttcgcttc tggtaaatat ttttatagag tttatttctt tcaggcaccg
     3481 caatacctag aaaagaaaat tgatggcgca tataggcttc catgggcctt gctttttaga
     3541 gtcttttgct gcttctagct cctaaagtaa atctgctaaa ctcatctaaa actcctcatg
     3601 ccccaccaaa tggtgctgaa aggcgtagac agccgcctgg gtacgatcgc tgacttcaag
     3661 tttggcaaga atgttggaca cgtgggtctt gaccgtcttg agagagataa agaggtcatc
     3721 tgcgatgcgc tgattttcgt agcccttggc gatgagttgg agaacatctc gctcacgcgc
     3781 agtcaattct tcatgaagtt ccatatgatt gcggtggtat tcaaccttct tgctaacctc
     3841 ttgctcaatg gccagctcgc cagcagctac cttactgacg gcatgaagca attcatctgc
     3901 actagaagtc ttgagcatat agcctttggc accagcatct aagactggca tgattttttc
     3961 attgtccaaa taagaggtca caatcaaaat cttggcttca ggccattctt taaggattgc
     4021 taaggtcgcg tcaattccat tcatctcagg catgacaata tccatgacaa tgacatctgg
     4081 acgcagttcc aaggccaagt caatcccttg agacccgttg gacgcctcac ccacaacttc
     4141 tacatcgtct tggaggtcaa agtagctttt caaacccaaa cggaccattt catggtcatc
     4201 tactagtaaa attttcatct ttactccttt atcattcctt atctaacagg ggaatacgga
     4261 tatcaactgc cagcccttgc ttgggagctg ttaataactg aaccgtccct gccatatctt
     4321 caacccgctc cttgatattt cgcagtccat aactcaagtc gtctaagctc cctaactgga
     4381 aaccaatccc attgtccacc accttcagtt gcaattcaac atctgtctga tagaggtaga
     4441 catctaggca agatgcctgg gcatggcgga gcgtattgct aatcaactct tgcaggatac
     4501 ggaagatatg ctcctcgatt ttcttaggca atttcgtcat attctgcttg agactaaccc
     4561 taagatcact cttgtcctca agctctttta agagaatttg aatcccttct atcaagctct
     4621 tctgctccag ttcaactggt cgcaaatgca agagcaaaac ccgcaaatcc ttctgggctg
     4681 tttctaaaat agctgtgaca ctctgcaact gggtctgcat cttttctcta tccaatttca
     4741 aagcctgctg actgataccc gataaaatca tgtgggccgc aaacaactcc tgactgactg
     4801 tatcgtgcaa atcccgagca attcgcttcc gttccttctc gatgatttcc tcttcctgag
     4861 caaggctatg attttcagct ttttgaagag cctctgtcaa aaggttaagt ttacctgata
     4921 aggacttgaa actggcatcc aaatcttgat ctacaacctg aactacttct tgccctgcca
     4981 ataaacgctt gagattagcc tgcatttttc ttagagaaag ctcttcgatc cctcgccaaa
     5041 acagggctaa gagacaggtc atggacatgc tgaaaaccaa caataaaaag acaaattttt
     5101 ctgttttttc gacatcgtgc aaaaagatag accagtcaaa atcaagtatt tccagcaagc
     5161 tgtgggagaa aaaaaagaca aataggaagg aggtgagagc aataatgaca taggcttgtt
     5221 ttttcatcct ctaaccacct ccacatcacc aatcatagtg gtcaagaaaa tcttgacact
     5281 cttgttactc ttgagatagt cttttgtttc ttgatgatag tgttcattgc ggagggctcg
     5341 cttgggctgg ttgaaaaaag tcaaatcccc atagagacag ttaacgctga gactgacttc
     5401 cacatctaca ggtacgatga ttttggtcgt tcctaccatc tttctgagga taatgacatt
     5461 gtcatgattg gttaagatga ccctctccag atgaatagtg tccttgccca tgaagcgaaa
     5521 gagattgata tcatcgaatt ggcaagtctg gtagcttgaa aaatgatgaa gatttccaaa
     5581 ccaacgattt ttctccttct taaccgtcac aacctcttca aaaaccaaat tggtctgctc
     5641 tttttcctgg ttcatcatcg ggtaaagaag aaagaggcta tagataaccg caacaaaaat
     5701 agctagaatc acaaaaggat tgagcataac gatgaaaaag aagagaatgg ttaccgctac
     5761 taaaagaaga ttatttccct ctttaccagt gtagtagcga atcaaaagca aaaagaggaa
     5821 tagtatcagc agaaaacgcg aaaaatgctc tgataccatc aaaatcagag ctcctgtcag
     5881 aagacaggct tcgataaata aaaagatttt aaattttctc ataggttcat cctctccctt
     5941 ctattttatc acaattcaaa aaagtcacct cagtctgagg atggaaaaaa aggcgctggt
     6001 tacgcctttt tcatctgatc atttgcttct tttaattttc cataaagaag atagtctact
     6061 ttttgtagat ctgctatggt ggcacagtta agggaacaca taatcaagcg tagatctgct
     6121 ttccagcctt ggacaatgcc aatcacttct tcaactgtgt aggtttcaac caattccaga
     6181 acggttcgtg acaatcccac agccttagca ccaaaaacca agcacttaat catatccagc
     6241 ggattccgaa cccctccact aaccaagagt tcgaccttat ctttccattc ttgggcattg
     6301 agaagggcct gcatggtaga ctgaccccat tgattgaggt aatcacgctg gctactacga
     6361 cggttttcga tataggcaaa gctggtgcca ccacgacccg ataggtccac tgtacgaaca
     6421 ccgaattcat aggctctttc gattgtcttg gcatccattc caaagcccac ttccttgagg
     6481 acaataggaa cgggaatttg cttgctataa tctgctagat gcgattgcca gcttctaaac
     6541 ttcctttctc cctcgggcat gagtaattcc tgcatgacat tgacatgcac ttgcaatagc
     6601 acaggattca tctcttctac agtctgaagt cctaactcga caggcttgtc caatccaata
     6661 ttggttccaa ggaggagatt gggatgacta gacttgacag aaaaagaatc atccgttgga
     6721 tttttgaggg ctgcgctata agaacccgtt acaaataaaa taccacagga ttccgccacc
     6781 tgagccagct tttgattgat ttctcttccc ttattacttc caccagtcat ggcattgata
     6841 taaaaaggaa agtcccactt tcgaccagca aactctgtcg aaagatcgat ttcatccaga
     6901 ttgtaaagag gcaaggaaga atgaatcagc tccacctcat caaagctatt ataggaactt
     6961 ttctgctcaa gggcatagag aatatgctcg tccttacgat ttgtcgtcat gtcctatcct
     7021 ttcttgatat aagagctcaa tccccagatc ggcccaacga ttttttaagg ttttggttga
     7081 ttgcgcatca aaactcaggg cgatgccaca gtcaccacca ccagcaccac tactcttggc
     7141 aacggcctgc aaatcttgac tggcttcttt caactgtcta agcaaaggcg tgtaaatatc
     7201 tgtactcaag ccttctaaaa gcttgctggc tacttctact tgctcgataa ctttttcggc
     7261 tttcccctgc tccaaggctt cgaccaaaga aaccaccgtt tcttttgagg aacttaaaaa
     7321 attttgattg atattttgct tgatttgctg gaccatgtga ctcgatacag ccacttcctt
     7381 ggtccatccc actaagaaat cacattctaa agttggtttc acttgtgaga taaaaaatcc
     7441 ccaatcacgc tccagaactg tcgccaagtt ttcttcttct aaccaagcag ccgccttctg
     7501 gcgatcaaat gactggtaga gaaccaaatc ctctgccaca atacaggcaa ggtcgcccat
     7561 ggaaccattg tctcctcgct tgagcaagac agcgctagtc agcttgaaca agagattctg
     7621 atcaaccgaa agattataga gagccaataa agccttgaca accaagacaa cgacgctgcc
     7681 actagaacct agaccaaact ttttcccttc tcgttccatt ttgccacaga tttttaggga
     7741 aaaaggtctt aaattctgac cgcgaacagc gaggaagtct cccatcaaag caatcgtttc
     7801 ttgaatcaag ctgtagtcag gattaggcct taagtccact gcgaaatcaa acatatctga
     7861 atagatacgg tagctgtcag aaaaagcaat ctcagccctc atatagatgg gaatatcctt
     7921 tatcaaagct aactgccctg gctctaaaat agcatattca cctgcccaat agagttttcc
     7981 gcaagtttta acagcaatca tcttgactca aatcctttgt ttttgacaca atcaagcgat
     8041 aacgatgacc gaaaatttct gataaatgct ccaagtcttt ctcctgacag aagaccttaa
     8101 cattgggacc agcatccatg gtaaagtagc aggcctctcc tttctcacga agctggcgaa
     8161 caaagtccat agcctcataa gaggcatccg tcagataaga aaaggctgga ctagcagtct
     8221 ttgtcgtagc atgcatagcc agggcatttt tctccgttaa ttctccaatc ttggcaaaat
     8281 cattttcctt gagataaatc agcatatcct gatagtcctt ctcagactga cgaacccagt
     8341 cgtcaaaagt cgtcgaggtt tccacacaaa gtttcatccc gtcacggcta gagattggtt
     8401 ttttcttgtc ctctagcacc aacataatca tagctagttt caagtctgtc tctacagggt
     8461 aaatttctcc actatcctta tcccaggctc ctagtggtcc ataaaaactc cgagaagaag
     8521 aacctgaggc aaatttggct tcctgtgcca actgacttct atccaatcca agcttgaaat
     8581 aagcattaca agccttgacc agggcggaca aaccactaga acttgaggac agacccgctg
     8641 ccgtaggcat attgttttga gtatcgatac ggacaaagcc ctcaccagct ggacgataac
     8701 ggtcaataat cttactcatc ttggcatgct cgacctcatt ttgtagctga ccattgatgt
     8761 aaaattcgtc agctgttaca ttggctggta aaggcgacaa ggtcgtctct gtatacatat
     8821 tttccaaagt tagagaaata ctgctagtag caggcaccac ctctttttct tttttctttc
     8881 cccaatattt gataatagca atatttgcgt aggaacgtac tgttacaggc tctctatcca
     8941 tgtctgaaca gctcctttct cttctaatct ttctgctagt tcttgtgcgt gtgtcaaatt
     9001 ggttaccaag gctatgatac aacctcctag cccaccaccg ctcatcttgg cacccagagc
     9061 accatggcta agagtcgttt caaccaaaaa gtctgcctca gggctactga ctccaatttc
     9121 ttttaaatgt aaatgcgctt gactgaggat ttgtcccagt ccttcagcat ctttttgtga
     9181 aatcgcaact tctgcttgct gggttaattc tcccaaggca tgcaaaaacg gtagggcatc
     9241 cttgccctta ttttgaacca cttggatggc ttcacgagta tgaccataaa cacccgtatc
     9301 ggcaatcacc aaataggcgg ataaatccat ctcaagttct gtaaatccta cgttcttgat
     9361 aaagcgaata ggttggtcac taagacaggt cttagcatcc aaaccactag gattcatatg
     9421 ggcaatcatt tcagctcgat tgaccaagat ttctagtaca tcatgaggca gatcagcctg
     9481 atagtagtca aatactgcac gaatggccgc tatgctgata gccgctgacg aacccatccc
     9541 ccgtttctca gggatagccg agtcaatctc acaacgaatg caggcttctg tgatattcaa
     9601 atactccagt gaggcataaa ccgccatgga caaggtatcc tcctcataaa ggcgccaagg
     9661 actctctgca ggaactacct tacaggtcac ctccacctcc aaaagaggca gggaaatggc
     9721 aggataaccg taaacgaccg catgttcccc tattaaaatt atcttactat gtgcctgacc
     9781 gacaccaact ttttttgtca ttttttcctt ttactagacg aaaaaacgtc ttatttttca
     9841 tacaagtatt aattctttcc tatctatttt attatatttt cacaaaaaaa gcgattgttt
     9901 ccattcacaa tcgcttcttt cattattgaa cccattcgcc attatagttg acagaatagc
     9961 catctacggt cgtattcact gccaaggcac ctgagctata agcgtagtac catctgccat
    10021 tgacctggaa ccaacctgtc gtcatagaac gacgaaagaa actccatacc attaagtaaa
    10081 gaggaaagtc gtgagggagc atgcgccatt gacaacctgt tttagtgacg tacaaagtct
    10141 cattaacaag tactcgtttc ggccatttat aggtgcggtg tttggagaaa tagggttcaa
    10201 tcttagccca ttcttgatcg tttaaatcag tatcatatac tttgcgtgtc ataactctag
    10261 tttaacattt tttgtgaata caggttctaa ataatcgacc acgaaaattt cttaagtgga
    10321 aaacgcctta tgaagtatgc tacgggaaag ttatgcactt aatttgacaa ttcaagatgt
    10381 aaaaatat
//
LOCUS       DBEROU010000057        10340 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.057, whole genome shotgun sequence.
ACCESSION   DBEROU010000057 DBEROU010000000
VERSION     DBEROU010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10340
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.057"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            32..7990
                     /locus_tag="ACL8CL_001900"
     CDS             32..7990
                     /locus_tag="ACL8CL_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HGS1331088.1"
                     /translation="MKSAQATYEETTTRRLSELTNLSNGKASKSELTQTAEELASRIA
                     SVQAGSSRNYFRNSRSRTFTTGGQAVYDYRTFIVPDFWKNSDRFKRDYVRISFDVTFP
                     VALVNDMPAMVHFSAHPWYAYRNLIFKGGTVERQHFEFTIDLSSSSEDYQTNNVFIRF
                     GTNYGFPAGLQVVIENAMLSVGNYFPAYQPAYEDQEDRVSVVESNFKQRADSLDAGVS
                     RLTEGLRTKADISSLNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEIL
                     NATKDKASKSELTQTAEELSSKIASVQASGRNLFLNSLFKQDISKTGIWTTSTYTAAI
                     DSESKYLGHKALKIIGLNPSGRDGGNPKVTYPALGQFGKVIPGSTTNQDVTISFYAKA
                     NKNGIMLRSRLGNIGYKTGNVTLSTEIKRYVVHISKGWTNESKQTTNEWLFNFNQEGT
                     VWIWMPKFEISDVDTSYSEAPEDIEGQISTVESTFKQRANSLEAGVSRLTEGLRTKAD
                     ISALNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKASKSE
                     LTQTAEELASKIASVHLGRRNLLKGTKELARYKPVSEYNGFKVIRTVAGATRYQDSYV
                     ERTVIPTAGTEYIAIFYARASENDYPVRCHFYNPNTVVSSENSSGYKSRSSDGLSIIR
                     LSTDWQLCWVKWTQTATDQAKTVIIGRHGPQVGGKEGVWVEICAPAIFEGNLAGDWSP
                     AYEDQDERVSAVESNFKQRADSLEAGVSRLTEGLRTKADISSLNVTAENIRQSVKSLE
                     TDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKASKSELTQTAEELSSKIASVQVGG
                     RNYIRGTKRMMLARGLWASGTFRPSGAGTAKTIDVSDSPVTGFDKAIRLTSSNARDQI
                     GIAQDGFYISQGTYTMSCWVKGRRGQKVKLQTYWQVNDNSGISPIFTLKDENWTKLSF
                     TSARNRAGVASIGYVYLVNAEVGEYLDVLAPQLEDGSLATSSKEAPEDIEGQISTVES
                     TFKQRANSLDAGVRSLTEGLRTKVDISSLNVTAENIRQSVKRLETDTQNKLNQKLSQA
                     EFEVRAGSIRQEILNATKDKASKSELTQTAEELSSKIASVQASGRNLFLNSLFKQDIS
                     KTGIWTTSTYTAAIDSESKYLGYNALKIIGLNPSGRDGGNPKVTYPALGQFGKVIPGS
                     TTNQDVTISFYAKANKNGIMLRSRLGNIGYKTGNVTLSTEIKRYVVHIPKGWTNESKQ
                     TTNEWLFNFNQEGTVWIWMPKFEISDVDTSYSEAPEDIEGQISTVESTFKQRANSLEA
                     GVNRLTEGLRTKVDISALNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQ
                     EILNATKDKASKSELTQTAEELSSKIASVQVGGINLLRNTASLLIGDRSKGCWMSASG
                     GNGRAISVEVLDPPKKMIKNMIRVIENTNGGNKDLTQLVGLRIGEKYTISCYARIASD
                     SPNANVNLLFRSWANNTDLNRKFQKSISHKNWQKYSFTFTADAIENSIQFGQSGAGII
                     EICAPKIESGTLATDYSEAPEDIEGQISTVESTFKQRANSLDAGVSRLTEGLRTKVDI
                     SALNVTAENIRQSVKSLETDTQNKLNQKLSQAEFEVRAGSIRQEILNATKDKADKTLV
                     VSEAGKLREEFSKMKVGGRNLWIKSKTVGAVIEKLPENHVTGQKECYRLENNSTLTFN
                     LEPDFSSRLYQKVTFSAWIKYENVVQGRNFWNVFNCFKHYLFRKNSETGVQSGPDYAT
                     LGMYKGSADWKYITFTYDYSEKTNFDQLKTSLRFNLEGATSGTAWVTGIKVEIGSVAT
                     DWSPAPEDADGLITEAKATFERTAQGLRTDLSAIQEYVNKDGQRQEALQRYTREESAR
                     QATAVRELVNRDFVGKATYQEDVKGINQRIEAVKTSANKDIASQIASYRQSVDGKFTD
                     ISSQITTYKQDVGGQISGLSNRLTSSEQGTTTQISNLSNRINSNKQGTDNQISNLKTQ
                     VATNKDNAERQMGRISDQVSANKANADSQFANVTNQLARKVETTDFQRVKETSKLYER
                     ILGNTENGIADKVARMALTNQLFQVEVGKYSVSGPNLIKNSDFKNATNEWGSTQNLGR
                     LVKHSFYHNGQKDLMRLSNATKNENFLYSHRFNLERNTDYVLNFRGFNNSALASYDVY
                     ILGRRAGESDGFTIVKKVVSSKKLSTSRCEDVSVTFNSGEMDNAYIRFDNNGSSSGTA
                     DLYITEVDLYKGYKPRTWQPHPEDAVADANKKLEATQTKMTQLAGSWVVENINSAGDI
                     ISGINLGANGHNRLVGKLTHITGETLIDRAVIKSAMVDKLKTANFEAGSVTTTILEAE
                     AVTAEKLKVDDALIKKLTATDAFIDQLISKRIFSTKVESVISSSTFLEAYQGRIGGFT
                     LGQFDQGGGRWISGVNQFSVGMGNGAGYGVRTAFWANWGNNWNYAGPKAWNVNTDGKM
                     YCRNEVGFYDQVDFSNSSRANFYGNTTFSRSPVFSNGIELGSKDVLGDGWNPKGGRNA
                     VVWWNQVGSGSLKYWMEQKSDRRLKENITDTAVKALDKINRLRMVAFDFIENKKHEEI
                     GLIAQEAETIVPRIVSRDPENPDGYLHIDYTALVPYLIKAIQELNQKIEKMEKTIA"
     gene            7987..8103
                     /locus_tag="ACL8CL_001901"
     CDS             7987..8103
                     /locus_tag="ACL8CL_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HGS1331089.1"
                     /translation="MNNNMLTNIALKAIQEFALENRKRTHRLENLENEHRTA"
     gene            8084..8287
                     /locus_tag="ACL8CL_001902"
     CDS             8084..8287
                     /locus_tag="ACL8CL_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331090.1"
                     /translation="MNTEQLNQALRMTISEISTTSTNSMITSNILSIQLDEQREENQR
                     LQARVDELEALLDEQTKPADKGE"
     gene            8290..8640
                     /locus_tag="ACL8CL_001903"
     CDS             8290..8640
                     /locus_tag="ACL8CL_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331091.1"
                     /translation="MAETIQNTDNLLDLTKITEPFDLASALRYMKENGEFIRCKNVSD
                     DFYMYRDVQKRPVIVNGRRQFKDVETVWAFNQWGGTIATINVAVLLNHEFYIMKFDAE
                     GNPDWTDPTVKPKE"
     gene            8650..9066
                     /locus_tag="ACL8CL_001904"
     CDS             8650..9066
                     /locus_tag="ACL8CL_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HGS1331092.1"
                     /translation="MQIEFFNFLRSVVQTEDGLVLYALALIVSMEIIDFVTGTIAAII
                     NPDIEYKSKIGINGLLRKISGVLLLMILIPASVLLPEKTGFAFLYSIYLGYIAFTFQS
                     LIENYRKLKGNVTLFQPIVKVFQRLLEKDDDTKKGE"
     gene            9070..9405
                     /locus_tag="ACL8CL_001905"
     CDS             9070..9405
                     /locus_tag="ACL8CL_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HGS1331093.1"
                     /translation="MQQITEIITNGAISILVVLAGVVVRAVKDYLVQKGGEKTIKIVE
                     ILAKNAVNAVEQVAAETGYKGDEKLAQARAKVRAELTKYNISMTDKDLDTFVESAVKQ
                     MNDAWKGQE"
     gene            9405..>10340
                     /gene="lytA"
                     /locus_tag="ACL8CL_001906"
     CDS             9405..>10340
                     /gene="lytA"
                     /locus_tag="ACL8CL_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HGS1331094.1"
                     /translation="MDIDRNRLRTGLPQVGVQPYRQVHAHSTGNRNSTAQNEADYHYR
                     KDPELGFFSHVVGNGRVMQVGPVNNGSWDVGGGWNTESYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPD"
BASE COUNT         3347 a         1969 c         2465 g         2559 t
ORIGIN      
        1 acttagtcgg actaagaatg aactggctgg cgtgaagtca gcgcaagcga cgtatgagga
       61 gacgacgact cgtagactgt cagaactgac caacttgtcc aatggtaaag ccagcaagtc
      121 agaactcacg caaacagctg aggagctggc tagtcggatt gcgagtgtgc aggcaggtag
      181 ttcacggaat tacttcagga attcacgttc aagaacgttc acaacaggag gtcaagcggt
      241 atacgactat cgaacattca tagttcctga tttctggaag aacagtgaca ggttcaagcg
      301 tgattatgtt cgcatatctt ttgatgtgac tttccctgtc gccctagtaa atgacatgcc
      361 tgctatggtg cattttagtg ctcatccatg gtatgcctac agaaacttaa tttttaaagg
      421 tggaactgtc gaacgccaac attttgagtt tacgattgac ttgtctagtt cttctgagga
      481 ctatcagact aataatgtgt tcattcgttt tggtactaat tatggatttc ctgctggtct
      541 gcaggtcgtc attgagaacg ctatgttatc ggttggtaat tattttccag cctatcaacc
      601 agcgtatgaa gaccaagaag accgtgtctc agtagtcgaa tccaacttta agcagcgtgc
      661 tgattcactc gacgctggtg taagccgtct gactgaaggc cttagaacta aagccgatat
      721 cagctcactc aatgtgactg ctgaaaatat ccggcaatct gtgaagagcc ttgagacaga
      781 tacgcagaac aagctaaatc agaagttgag tcaggctgaa tttgaggtgc gagccggctc
      841 tatccgtcag gaaatcctga acgcaaccaa ggataaagcc agcaagtcag aactcacgca
      901 gacagctgag gagctctcta gtaagatagc gagtgtgcag gcatccggtc gaaatctatt
      961 cttgaactca ctattcaagc aggatatttc aaaaacagga atttggacaa cgagtacata
     1021 tacggctgct atcgatagcg aaagtaagta tcttggacac aaggctctta aaattatagg
     1081 tctgaatcca tctggccgtg atggaggtaa tcccaaggtt acttatccag ctctgggtca
     1141 attcgggaaa gtaattcccg gaagtacgac taatcaagat gtaaccatta gtttttatgc
     1201 taaggcaaat aaaaatggaa taatgctaag atctcgatta gggaatatcg gatataaaac
     1261 tggaaatgtg acattgtcga cagaaattaa gcgatatgtt gtccatattt caaaaggttg
     1321 gacaaacgaa tccaagcaga ccacaaatga atggttgttc aatttcaacc aggaaggaac
     1381 cgtttggatt tggatgccga agtttgaaat aagcgatgta gatacttctt attcagaagc
     1441 tcctgaagat atagaaggtc agatttcaac agttgaatcg acctttaaac aacgagccaa
     1501 ctcgctcgaa gctggtgtaa gccgtctgac tgaaggtctt agaaccaaag ccgatatcag
     1561 cgcactcaac gtgactgctg aaaatattag gcagtcggtg aagagtcttg agacagacac
     1621 gcagaacaag ctaaatcaga agttgagtca ggctgaattt gaggtgcgag ccggctctat
     1681 ccgtcaggaa atcctgaatg caaccaagga taaagccagc aagtcagaac tcacgcagac
     1741 agctgaggag ctagctagta agatagcgag tgtgcactta gggcgcagaa atctgctgaa
     1801 aggcacaaaa gagcttgcga gatacaagcc ggttagtgaa tataatggtt ttaaagttat
     1861 cagaacagtc gcaggagcaa ctagatatca ggatagctat gtggaaagaa ccgttatacc
     1921 aacggctggg acagagtata tagctatctt ttatgcacga gccagtgaaa atgactatcc
     1981 tgtgcgctgt catttttaca atcctaacac ggttgtatca tcagaaaaca gcagcggata
     2041 taagtcaagg tcgtcagatg gcttgtctat tatccgtctc tcgacagact ggcagttgtg
     2101 ctgggttaaa tggacccaaa ccgcaacaga tcaagccaag acggtcatca ttggccgcca
     2161 tggccctcaa gtaggcggta aagagggggt atgggttgaa atctgcgccc ctgccatttt
     2221 tgagggaaat cttgcaggtg actggtcacc agcatacgaa gaccaagacg aacgtgtctc
     2281 agcggtcgaa tccaacttta aacagcgtgc tgattcactc gaagctggtg taagccgtct
     2341 gactgaaggc cttagaacca aagccgatat cagctcactc aacgtaactg ctgagaatat
     2401 ccggcaatct gtgaagagtc ttgagacaga cacgcagaac aagctaaatc agaagttgag
     2461 tcaggctgaa tttgaggtgc gagccggctc tatccgtcag gaaatcctga atgcaaccaa
     2521 ggataaagcc agcaagtcag aactcacgca gacagctgag gagctctcta gtaagatagc
     2581 gagtgtgcag gtcgggggta ggaattatat ccggggtaca aagcgcatga tgctagccag
     2641 aggattgtgg gcatcaggta cttttagacc gtcaggcgct gggacggcaa agacgattga
     2701 tgtatcagac agtccagtaa ctggctttga taaagcgata cgattgacct caagcaatgc
     2761 tagagaccaa ataggtattg ctcaagatgg attttatatc tcgcaaggca catacacgat
     2821 gtcttgttgg gtcaaaggca gaagaggtca aaaggtcaag ctacaaactt attggcaagt
     2881 caatgataat tcgggtattt cgcccatctt tacattaaag gatgaaaatt ggacaaagct
     2941 atcgtttact agcgctagaa atagggctgg agtcgcatca attggctatg tgtatctcgt
     3001 aaatgctgaa gtcggagaat atttagatgt tcttgcgccc cagctggaag acggaagttt
     3061 ggcaacaagc tcaaaagaag ctcctgaaga tatagaaggt cagatttcaa cagtagaatc
     3121 gacctttaaa caacgagcca actcgctcga cgctggtgtg agaagcttga ctgaagggct
     3181 tagaaccaaa gtggatatca gctcactcaa tgtgactgct gaaaatatcc ggcaatctgt
     3241 gaagaggctt gagacagaca cgcagaacaa gctaaatcag aagttgagtc aggctgaatt
     3301 tgaggtgcga gctggctcta tccgtcagga aatcctgaat gcaaccaagg ataaagccag
     3361 caagtcagaa ctcacgcaga cagctgagga gctctctagt aagatagcga gtgtgcaggc
     3421 atccggtcga aatctattct tgaactcact attcaagcag gatatttcaa aaacaggaat
     3481 ttggacaacg agtacatata cggctgctat cgatagcgaa agtaagtatc ttggatataa
     3541 cgctcttaaa attataggtc tgaatccatc tggccgtgat ggagggaatc ccaaggttac
     3601 ttatccagct ctgggtcaat tcgggaaagt aattcccgga agtacgacta atcaagatgt
     3661 aaccattagt ttttatgcta aggcaaataa aaatggaata atgctaagat ctcgattagg
     3721 gaatatcgga tataaaactg gaaatgtgac attgtcgaca gaaattaaac gatatgttgt
     3781 ccatattcca aaaggttgga caaacgaatc caagcagacc acaaatgaat ggttgttcaa
     3841 tttcaaccag gaaggaaccg tttggatttg gatgccgaag tttgaaataa gcgatgtaga
     3901 tacttcttat tcagaagctc ctgaagatat agaaggtcag atttcaacag tagaatcgac
     3961 ctttaaacaa cgagccaact cgctcgaagc tggtgtgaat cgtctgactg aaggccttag
     4021 aaccaaagtg gatatcagcg cactcaacgt gactgctgaa aatatccggc aatctgtgaa
     4081 gagtcttgag acagacacgc agaacaagct aaatcagaag ttgagtcagg ctgaatttga
     4141 ggtgcgagcc ggctctatcc gtcaggaaat cctgaacgca accaaggata aagccagcaa
     4201 gtcagaactt acgcagacag ctgaggagct ctctagtaag atagcgagtg tgcaggtcgg
     4261 aggaatcaac ctcttgcgta atactgcgag tttgttgatt ggcgatcgtt caaaaggatg
     4321 ttggatgagt gcaagcggag gaaatgggcg agcgattagc gtagaagttt tggatcctcc
     4381 caaaaaaatg ataaaaaaca tgattcgtgt tattgaaaat acgaatggtg gaaataaaga
     4441 tttaactcaa cttgttggat tgcgaattgg tgaaaagtac acgatttctt gttatgcaag
     4501 gattgctagc gatagcccaa atgcaaacgt gaacttgcta tttcgttcgt gggcaaataa
     4561 taccgattta aatcgcaaat ttcagaaatc catctctcat aaaaattggc aaaaatattc
     4621 atttacattt actgctgatg caattgaaaa ttcaattcaa tttggacaat ctggcgcagg
     4681 aattatcgaa atatgcgctc cgaaaatcga atcaggaacg ttagcgactg attacagcga
     4741 agctcctgaa gatatagaag gtcagatttc aacagttgaa tcgaccttca aacaacgagc
     4801 caactcactc gacgctggtg taagccgtct gactgaaggt cttagaacta aagtggatat
     4861 cagcgcactc aacgtgactg ctgaaaatat taggcagtcg gtgaagagtc ttgagacaga
     4921 cacgcagaac aaactaaatc agaagttgag tcaggctgaa tttgaggtgc gagctggctc
     4981 tatccgtcag gaaatcctga acgcaaccaa ggataaagca gataagactt tagttgtatc
     5041 tgaagctggg aaattgcgtg aagaattttc aaaaatgaag gtgggaggac ggaatctatg
     5101 gataaaatcc aagacggttg gagctgtaat tgaaaaatta cctgaaaacc acgtcacagg
     5161 tcaaaaagaa tgctataggc tagagaacaa ctctacttta acgttcaacc ttgaaccaga
     5221 tttcagctca aggttgtacc aaaaagttac ttttagcgct tggatcaagt acgaaaatgt
     5281 agtccaaggt cgaaattttt ggaatgtatt taattgcttc aaacattatc tttttagaaa
     5341 aaatagtgag accggagtac agagtggtcc agattatgct acgcttggta tgtataaagg
     5401 ttcggcagat tggaaatata ttacattcac ttatgactac tctgaaaaaa caaattttga
     5461 tcaattgaag acatcattgc gattcaatct tgaaggtgct acaagcggta cagcttgggt
     5521 aacaggaatc aaggttgaaa tcggtagtgt ggcgacggac tggagtcctg cgcctgagga
     5581 cgctgatggt ctcatcactg aggctaaggc tacctttgag cggacagctc agggcttgcg
     5641 aaccgactta tcagctattc aggaatatgt aaataaagac ggtcagcgac aggaagccct
     5701 acagcgctat actcgtgagg agagcgcgag acaagcgaca gcagtccgtg agctggtcaa
     5761 tcgtgatttc gttggtaagg ctacttatca agaagatgtt aagggtatca atcagaggat
     5821 tgaagctgtt aaaactagtg cgaataaaga catcgctagt caaatcgcta gctatcgtca
     5881 atctgtagat ggtaagttca cggatatttc aagtcagata actacttata agcaagatgt
     5941 gggcggtcaa atcagtggtc tatcaaatag acttacaagt agtgagcaag gaaccactac
     6001 tcagatttca aatctttcaa atcggataaa cagtaataaa caaggcacag ataatcagat
     6061 ttcaaattta aagactcagg tcgctacaaa caaggataat gctgaacgac aaatgggtag
     6121 aatatctgat caggtttctg caaacaaagc gaatgctgat agtcaatttg cgaatgtgac
     6181 caatcaacta gcgcgaaaag tagagactac tgacttccag cgtgttaagg aaaccagtaa
     6241 actttacgag cggattttgg gcaatactga aaatggaatt gcggataagg ttgctcgcat
     6301 ggctctgact aatcaactgt ttcaggttga ggttgggaaa tatagtgtaa gcggccctaa
     6361 cctcattaag aatagtgatt ttaaaaatgc tacgaatgaa tggggctcaa ctcaaaattt
     6421 aggaagattg gttaagcata gcttttatca caacgggcag aaagacctta tgcgtttaag
     6481 taatgcaact aaaaacgaaa actttttgta tagtcaccgt tttaatcttg aacgaaatac
     6541 tgactatgta ctgaatttta gaggatttaa caacagtgct ctagcaagct atgatgttta
     6601 tattttggga cgaagagcag gcgagagcga tggattcaca atcgttaaga aagttgttag
     6661 cagcaagaaa ctatctacct ctagatgcga agatgtctca gtaactttta attccggaga
     6721 aatggataat gcttacattc gttttgataa caatggctca tcatcaggaa cagctgattt
     6781 gtatattaca gaagttgact tgtacaaagg ttataaacct agaacatggc aaccacatcc
     6841 agaagatgca gtcgcagatg cgaataagaa gcttgaagca acgcaaacaa aaatgactca
     6901 actagctggc tcatgggtag ttgaaaacat caactcggct ggagatatca tctctggaat
     6961 caatcttggc gccaatggac ataaccgctt agttgggaaa ttgacccaca tcactggaga
     7021 gaccctgatt gacagagcag tcatcaagtc tgccatggtt gataagctca aaacggccaa
     7081 ttttgaagct ggttcggtca cgactacgat attagaagct gaagcggtaa ctgctgagaa
     7141 gttgaaagtt gacgatgcgc ttattaaaaa attaactgca actgatgctt ttattgacca
     7201 actgatatct aaacgtatct tctctactaa ggttgagtcc gtcatttcta gctcaacctt
     7261 cctagaagcc tatcaaggcc gaatcggtgg attcacactt ggtcaatttg accagggtgg
     7321 cggtcgctgg atttcaggtg tcaatcagtt ctctgttggt atggggaatg gtgccgggta
     7381 tggagtccgg acagccttct gggcgaactg gggaaataat tggaactatg ccggacctaa
     7441 agcatggaac gtcaatactg atgggaaaat gtactgtagg aatgaagtcg gtttttatga
     7501 tcaagtggat ttttcgaatt catcgagagc aaacttctat gggaatacta ctttttctcg
     7561 ttctcctgtg ttttcaaatg gtatcgaact tggaagtaaa gatgtgcttg gtgatggttg
     7621 gaatcccaaa ggcggaagga atgcggttgt ttggtggaat caggtcggta gcggtagctt
     7681 gaagtattgg atggaacaaa aatcagatag acgcttaaaa gagaacatca cagatacagc
     7741 tgtgaaagcc ttggataaaa tcaacagatt aagaatggtt gcatttgatt tcatcgaaaa
     7801 taagaaacat gaggagattg gtctaatagc tcaagaggct gaaaccatcg ttccaagaat
     7861 tgtctcacga gatcctgaga atccagatgg ctatctacat attgactata ccgctttagt
     7921 tccttactta atcaaggcta ttcaagaatt aaatcaaaaa atagaaaaaa tggagaaaac
     7981 aatagcatga ataacaacat gttgaccaat atcgcactta aagcaattca ggagtttgct
     8041 cttgaaaata gaaaacgaac acacagattg gagaacttag aaaatgaaca cagaacagct
     8101 taaccaagcc ttacgaatga caattagtga aatatcaaca acttcaacaa attcgatgat
     8161 tacaagtaat atcttgagta ttcagttgga tgagcaaagg gaagaaaatc aaagacttca
     8221 agcacgagtg gatgagctgg aagctctgct tgatgaacaa actaaaccag cagacaaagg
     8281 agaatagaca tggcagaaac aattcaaaac acagataact tactagacct tacaaaaatc
     8341 acagaaccat ttgatcttgc gagtgctttg cgctacatga aagaaaatgg agagttcatt
     8401 cgttgcaaga atgtaagcga tgacttctat atgtatcgtg acgttcaaaa acgtcctgtg
     8461 attgtaaatg gccgtcgcca attcaaggat gttgaaaccg tttgggcgtt caatcagtgg
     8521 ggtggtacaa tcgcaacaat caacgtagcc gttctgttga atcatgaatt ctatatcatg
     8581 aaatttgatg cagagggcaa tcctgactgg acggatccaa cggtaaaacc taaagaatag
     8641 gaggtgtgta tgcaaattga atttttcaat tttctaagaa gtgtcgtaca gactgaagat
     8701 ggtttggtct tgtacgctct agcactgatt gtctcaatgg aaatcattga ttttgtgaca
     8761 gggacgattg cggcgattat caatcctgac atcgagtaca agagcaaaat cggcattaac
     8821 gggctccttc gtaagatttc aggggttctc ttactgatga tcctcattcc ggcgtccgtt
     8881 ttgttgcctg aaaagacagg ttttgcattc ttgtactcaa tctatctcgg gtacatcgca
     8941 tttacttttc aatctctcat tgaaaattac cgcaaattaa aaggaaatgt tactcttttt
     9001 cagccgattg taaaagtatt tcagcgatta cttgaaaaag atgatgatac gaaaaaagga
     9061 gaataacaaa tgcaacaaat tactgaaatc attactaatg gagcaatcag catcctagtc
     9121 gttttggcag gggttgtagt tagggcagtc aaggactacc tggttcaaaa aggtggagaa
     9181 aagaccatca aaatcgttga aatcttggcc aaaaatgcag taaatgccgt ggagcaggta
     9241 gctgctgaaa ctggctacaa gggagatgaa aaactggcac aggctcgtgc taaagtccgt
     9301 gctgagctta caaaatacaa tatcagtatg acggacaagg acttagacac ctttgtggag
     9361 tcagccgtga agcagatgaa cgacgcttgg aaaggacaag agtaatggat atcgatagaa
     9421 acagactacg aacaggcttg ccacaggttg gggtgcagcc ttatcgacaa gtacacgccc
     9481 actcaacagg caaccgtaac tcaacagctc aaaatgaggc tgattaccac tatagaaagg
     9541 accctgaact agggttcttt tcacatgttg tcggaaatgg ccgtgtcatg caagtaggtc
     9601 ctgtgaacaa cggaagttgg gatgttgggg gcggttggaa tactgagagt tacgcagcgg
     9661 ttgaactgat tgaaagccat tcaactaaag aagagttcat gacggactac cgcctttata
     9721 tcgaactctt acgcaatcta gcagatgaag caggtttgcc gaaaacgctt gatacaggga
     9781 gtttagctgg aattaaaacg cacgagtatt gcacgaataa ccaaccaaac aaccactcag
     9841 accacgttga cccttatcca tatcttgcta aatggggcat tagccgtgag cagtttaagc
     9901 atgatattga gaacggcttg acgattgaaa caggctggca gaagaatgac actggctact
     9961 ggtacgtaca ttcagacggc tcttatccaa aagacaagtt tgagaaaatc aatggcactt
    10021 ggtactactt tgacagttca ggctatatgc ttgcagaccg ctggaggaag cacacagacg
    10081 gcaactggta ctggttcgac aactcaggcg aaatggctac aggctggaag aaaatcgctg
    10141 ataagtggta ctatttcaac gaagaaggtg ccatgaagac aggctgggtc aagtacaagg
    10201 acacttggta ctacttagac gctaaagaag gcgccatggt atcaaatgcc tttatccagt
    10261 cagcggacgg aacaggctgg tactacctca aaccagacgg aacactggca gacaagccag
    10321 aattcacagt agagccagat
//
LOCUS       DBEROU010000058        10286 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.058, whole genome shotgun sequence.
ACCESSION   DBEROU010000058 DBEROU010000000
VERSION     DBEROU010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10286
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.058"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            85..552
                     /gene="tadA"
                     /locus_tag="ACL8CL_001907"
     CDS             85..552
                     /gene="tadA"
                     /locus_tag="ACL8CL_001907"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HGS1331095.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGATGSLYDILTDERLNHRVEVETGILEDECAAIMQDFFRNRRKK"
     gene            585..682
                     /gene="ffs"
                     /locus_tag="ACL8CL_001908"
     ncRNA           585..682
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACL8CL_001908"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            739..1182
                     /locus_tag="ACL8CL_001909"
     CDS             739..1182
                     /locus_tag="ACL8CL_001909"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HGS1331096.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNPRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            1184..1699
                     /locus_tag="ACL8CL_001910"
     CDS             1184..1699
                     /locus_tag="ACL8CL_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HGS1331097.1"
                     /translation="MKIIFVRHGEPDYSELEERSYIGFGINLAPLSEMGRQQVQKLSK
                     NPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            1713..3074
                     /gene="radA"
                     /locus_tag="ACL8CL_001911"
     CDS             1713..3074
                     /gene="radA"
                     /locus_tag="ACL8CL_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016893792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HGS1331098.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            3147..3644
                     /locus_tag="ACL8CL_001912"
     CDS             3147..3644
                     /locus_tag="ACL8CL_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HGS1331099.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            3669..4338
                     /locus_tag="ACL8CL_001913"
                     /pseudo
     CDS             3669..4338
                     /locus_tag="ACL8CL_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
     gene            4483..5451
                     /locus_tag="ACL8CL_001914"
     CDS             4483..5451
                     /locus_tag="ACL8CL_001914"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HGS1331100.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            5569..6514
                     /locus_tag="ACL8CL_001915"
                     /pseudo
     CDS             5569..6514
                     /locus_tag="ACL8CL_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175244.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            7012..9645
                     /gene="polA"
                     /locus_tag="ACL8CL_001916"
     CDS             7012..9645
                     /gene="polA"
                     /locus_tag="ACL8CL_001916"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HGS1331101.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDY
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVSESLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
     gene            9730..10167
                     /locus_tag="ACL8CL_001917"
     CDS             9730..10167
                     /locus_tag="ACL8CL_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HGS1331102.1"
                     /translation="MSQEVINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLEAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
BASE COUNT         2854 a         1780 c         2563 g         3089 t
ORIGIN      
        1 tagagtcacc gcattcggtg gctttttttg tgttgggatt catgatataa taataaaatc
       61 gataagtagg aaaagagaaa agagatgtat tatacgcttg aagaaaaaga agtctttatg
      121 agggaggctt tgagagaggc tgagattgct cttgaacacg atgaaattcc aattggttgt
      181 gtgattgtca aagatgggga aatcattggt cgtgggcata atgcgcgtga ggaattacag
      241 cgagcggtta tgcatgcgga aattatggct atagaggatg cgaacttgag tgaggagagc
      301 tggcgcttgc tggattgcac actttttgtg accattgaac cttgtgtcat gtgtagtggg
      361 gcgattgggc ttgcccgcat tccaaatgtg gtctatgggg ctaaaaacca gaaatttggc
      421 gctactggaa gtttgtacga tatcttgaca gatgagcgtc ttaaccatcg tgtagaggtt
      481 gaaacgggaa ttttggaaga tgaatgtgca gctatcatgc aggacttttt tagaaataga
      541 cggaaaaaat aattttgctt ttaaaatgaa taggaatgtg atataataaa tagtggagca
      601 acagttctgc gtgaagcggg tcaggggagg aatccagcag ccctaagcga tttgaattgt
      661 gtgctctttt tttcgtgctt tttccgaata aataagatag aataatctag aataaatgat
      721 aatagaaaag agaaaattat gaaaattcgt ggttttgaat tggtttcgag ttttacagat
      781 gaaaatttat tgcccaagcg tgagacagcg catgcggctg gttacgactt aaaggttgct
      841 gtgcgtacag ttgttgcgcc aggagagatt gtcttggttc cgacaggggt taaggcttat
      901 atgcagccga ctgaggttct ctacctctat gatcgttctt caaatcctcg taagaagggc
      961 ttggttttaa ttaactcagt tggggtcatt gatggggatt attatggaaa tcctggaaat
     1021 gaagggcata tttttgcgca gatgaagaat atcacagacc aagaggttgt tcttgaagtt
     1081 ggggagcgta ttgtccaggc tgtttttgct actttcttaa ttgcagatgg agatgcagct
     1141 gatggcgttc gaactggtgg atttggatcg acagggcact agaatgaaga ttatctttgt
     1201 acgtcatggg gagccggatt acagtgagtt agaggagcgt tcttatatag gatttgggat
     1261 aaatttggca cccttgtctg agatgggacg gcagcaagtc cagaaattga gcaaaaatcc
     1321 tttactctcg tcagctgaaa taatcgtatc ttctgcagtc acaagagctt tagaaacggc
     1381 ttcgtatgtg gtctgtgcta cgggtcttcc tttaagagta gagcctttat tacatgaatg
     1441 gcaggtctat aaaacaggaa tagaaaactt tgaaacagct agaagactgt ttttagaaaa
     1501 caagggggag ttgcttccta atagtcctat tcaatatgag acagctacgg aaatgaagtc
     1561 tcggtttcta gaatgtatgt ctaagtatcg agaacatcag actgtggtag ttgttgctca
     1621 tcgaatgctc atgcgccagt ttgtgccaaa tgagaagatt gatttttgcc aagtgattga
     1681 gtgtgagtta gagatataga aagaggtttg tcatcgcaaa gaaaaaagcg acatttgtat
     1741 gtcaaaattg tgggtataat tcccctaaat atctgggacg ttgccccaac tgtgggtctt
     1801 ggtcttcttt tgtggaagag gttgaggttg ccgaggtcaa gaatgcgcgt gtgtccttga
     1861 caggtgagaa aaccaagccc atgaaactag ctgaggtgac ttccatcaat gtcaatcgaa
     1921 ccaagacgga gatggaggaa ttcaaccgtg tgcttggagg cggagtggta ccaggaagtc
     1981 tcgtcctcat cggtggggat cctgggattg ggaaatcaac tcttctccta caagtctcaa
     2041 cccagttgtc ccaagtgggg acagttctct atgtcagtgg ggaggagtct gcccagcaga
     2101 ttaaactacg tgcagagcgc ttaggtgata ttgatagtga gttttatctc tatgcagaga
     2161 ccaatatgca gagtgttcgt gcagaagtgg agcgtatcca gccagacttt ctcattattg
     2221 attccatcca gaccatcatg tctcctgaga tttcaggggt gcaggggtct gtttctcagg
     2281 tgcgtgaagt gaccgctgaa ctcatgcagt tggccaagac caataacatt gccatcttta
     2341 tcgtaggtca tgtgaccaaa gaaggaacct tggctgggcc tcgtatgttg gagcatatgg
     2401 tggatacggt gctttacttt gaaggggagc gtcaccacac ctttcgtatt ttgagagcgg
     2461 tcaaaaatcg ttttggttcc actaatgaga ttgggatttt tgagatgcag tcgggcggct
     2521 tggttgaggt actcaatccg agtcaagttt tcctagaaga gcgtttggat ggggcgactg
     2581 gttcctccat cgttgtaacc atggaaggga cgcgtccgat tttggcggag gttcaggctt
     2641 tggtaacacc gaccatgttt ggaaatgcca agcgtactac gacaggactt gattttaacc
     2701 gtgctagctt gattatggct gttttggaaa aacgggcagg gcttctcttg caaaatcagg
     2761 atgcctatct caaatctgct ggtggtgtta aattggatga acctgcgatt gacttggctg
     2821 ttgcagttgc tattgcttcg agctacaaag acaagccaac taatcctcag gaatgttttg
     2881 tcggagaact gggcttgaca ggagagattc ggcgcgtgaa tcgtattgag caacgcatca
     2941 acgaagctgc taaactgggc tttactaaga tttatgtacc taagaattcc ttgacaggaa
     3001 tcactctgcc taaggaaatt caggtcattg gcgtgacaac gattcaggaa gtcttgaaaa
     3061 aggtctttgc ataatccgtg acaaattctc ttaaaaatga taagatagga gaaatatttg
     3121 actatcaaat tttcaaggag ggaatcgtgt cgtattttga acagtttatg caagccaatc
     3181 aggcttatgt tgccctacat gggcagttaa atctgccact taaacccaaa acaagagtag
     3241 ctattgtgac ctgtatggac tctcgtctgc acgttgcgca agctctgggc ttggcacttg
     3301 gggatgctca tatcttgcgg aatgcaggtg gtcgagtgac tgaagacatg attcgttcgc
     3361 tagttatttc ccagcaacaa atggggacaa gagagattgt ggtattgcac catacagact
     3421 gtggtgctca gacctttgaa aatgaacctt ttcaggagta tttaaaagag gaattaggtg
     3481 tagatgtgtc agaccaggac ttcttgccct tccaagatat agaagagagt gtacgcgagg
     3541 atatgcaact gcttatcgag tctcccctaa taccagacga tgtcattatc tctggtgcca
     3601 tttacaatgt tgatacagga agtatgacag tcgtagaatt ataaatactt catttagaaa
     3661 gaaagtgtat gaagaaaagc agtattttat ttatttttat tttattgcta tgtattggtt
     3721 tacagtatga aaccatctac tatacggacg gttcgaggtc aggtgcggaa tatggactaa
     3781 tgggagtttc tatctttcta gctctctttt acatgattcc ggctctttat tttctcttcc
     3841 gtattgggaa aaaatgggaa ttgccaaaga aggttttgat tctgtcttta ttgggaggga
     3901 tgttcctttc aggctggttg tctagttttg ctaatactta tatccatgat ttactggggg
     3961 ttcttttccc agatagtaca tttttaaatg cctttgaaag tgctattgtg gctcctttgg
     4021 tagaagaacc cttgaaatta ttgccacttg tttttgtttt ggctttgatt cctgtgcgaa
     4081 aattaaaatt tttgttttta cttggaattg cctctcactg gactttttca ggtctagctg
     4141 tagtaggtgt ttacttgctt tacagagcct ataaaggaca gaaggttggc aagaaacagg
     4201 gtcttattgt ttaactctcc ttttgtggag ttggaaacag agttgccttt agcgattcca
     4261 gtggttacgg ctattgctct ctatggttat tatcatgctt attgctttgt tgagaaacac
     4321 aatgagttga tgacctagaa tatttttcaa aagaatgatg caagggtaca aatatggtgc
     4381 ccttctttta tttttgattg aaaaatagtg caaaaagcgc tacaatggta gatggaaaat
     4441 cttgtgaaaa gcacaagcga tacatatata ccggaggaaa tcatgtcttt ttctgattta
     4501 aagctgtttg ccctttcttc taataaagaa ttggcagaac gtgtggcgca ggagattggg
     4561 atagagttgg ggaaatcaag tgttcgccaa ttttcagatg gagagattca ggtcaacatt
     4621 gaagaatcaa tccgtgggaa acacgtcttt atcctacaat caactagttc gcctgtaaat
     4681 gacaatctgc ttgaaatttt gattatggta gatgctttga agcgtgcgag tgcagaatct
     4741 gtcaatgttg tcatgcctta ctatgggtat gcacgtcagg atagaaaggc gagagcgcgt
     4801 gagccaatca cttcaaaact tgtcgcaaat atgcttgaag tagctggagt ggatcgttta
     4861 ttgaccatcg acttgcatgc tgcgcaaatt caaggattct tcgatattcc tgtggatcat
     4921 ttgatgggtg ctcctctaat tgcagattat tttgagcgtc gtggtatggt tggttctgac
     4981 tatgtggttg tcagcccaga ccatggaggg gtgactcgtg ctcgtaagtt ggcagaattt
     5041 ttgaaaacat ctatcgctat tattgataaa cgtcgtagcg ttgataagat gaatactagt
     5101 gaagttatga acatcatcgg taaggttgaa ggcaagactt gtatcttgat tgatgatatg
     5161 attgataccg ctggaacgat ttgtcatgcg gcagatgctc ttgcggaagc tggtgctgtt
     5221 gaagtctatg caagctgtac gcacccagtt ctttctggtc ctgctacgga caatatccaa
     5281 aaatcagcta ttaagaaatt ggttgttttg gataccatct atctgccaga agagcgtttg
     5341 attgataaga ttgagcagat ttcaatcgct catctcctag gggatgctat cgtacgtatt
     5401 catgaaaaac gaccactttc tccacttttc gatattgaga aaaaaattta atgaccaagc
     5461 ctgagatgat tctcaggttt tttcgtcttt tttccgaata aatagatagt agcggtgaat
     5521 ctagtaaagc tagatttaaa actgtgatat aataaaaaga gaagaaatat gactgtacgt
     5581 catccgggca ttagcccaac caatgacttg gtagctaaga aaatctttag caatccagaa
     5641 atcacttgtc aatttattcg cgatatgctg gatttgccag ccaaaaatgt gaccattttg
     5701 gagggtagca atattcacgt attactctcc atgccttact cggtgcagga tttttatacc
     5761 agtatagacg tcttggggga gttggataac agtactcaag taattattga gattcaagtc
     5821 catcatcaga atttttcatc aatcgcttgt gggcttacct gtgcagtcag gttaatcaaa
     5881 atcttgaaaa aattcgtcag cgagaaggtg atactcacta gagctacaaa cacatcgctc
     5941 ctgtttacgc cattgctatc gtggatagta attatttctc agatgacctg gcttttcata
     6001 gctttagtat gcgcgaagac acaacaggtg aggtattggc gattaccaac aatggacagg
     6061 aaaaccatct ggttaagatg gcattcttgg aattaaaaaa tacagagaaa ccagcaaaga
     6121 caaggttcgc aagccatggt tggagttttt cggcaacaag ccctttaccc agcaaccgca
     6181 acgagccatt acccaagcaa atcaactgct ggactacaag agctggtccg aagaggacag
     6241 gaaaatgttt agtcaactac atatgcgaga agaacaagtc ttgttagcac aggactatgc
     6301 cttggaaact gctagggctg aaggccttga acaaggactg gagcgtggta ttgaacgtgg
     6361 tcgtgcagag ggtcgtgaac aaggacgaga agaaggcctt gaagagggat taaaagtagg
     6421 tttagtaaat ctagtacgtc aaggtcttct aacacctgag gttgcgagtg agcaattagg
     6481 catgaccgtg gctgagtttg agtccttgct atgagttcat actttatata gtagaaagtt
     6541 gatgggatgg caagtttatt atcaaaatac ttgtcatttt tgcttgacat ttctatcttt
     6601 ttataagata gtattagcga agtaagttat gtctcgctat tcatacagtt tggatagtcg
     6661 caagattgtc tttgagattt cctgttttaa agagaaaaaa gcctctttga cgctattttt
     6721 tcatcttttt gaaagttcta taatgaagtt agccacccag ccctcctttt cctccttcta
     6781 tagtgagttg aaataagatg tgaataactc tatcaggaaa gtcaaattaa tttatagaaa
     6841 tattttagca gtcaaggtgt actattatag attcaatata ttatagcaca tttttaaatc
     6901 taggtttact aaattctctg gctctatcta tttattcgga aaaagtttga aaaatacttg
     6961 ctctagctct tcccaatggt attttttgat tctttccttt ataatgggtg tatggataag
     7021 aaaaaattat tattgattga tgggtcttct gtagcttttc gggcgttttt tgcgctgtat
     7081 cagcagttgg accgttttaa gaatgcggct ggtttgcata ccaatgcgat ttatggtttt
     7141 cagttgatgt tgagtcattt gttggagcgg gttgagccga gtcatatttt ggtggctttt
     7201 gatgcgggaa agacgacctt ccggacagag atgtatgcgg actataaggg tggtcgggcc
     7261 aagactcctg atgagtttcg tgagcaattt cctttcattc gtgagttgct ggattatatg
     7321 gggattcgtc actatgagct ggctcagtat gaggcggatg acatcattgg gacgctggat
     7381 aagctagcag agcaggatgg ttttgatatt accattgtca gtggggacaa ggatttgatt
     7441 cagctgacgg atgagcatac ggtggttgaa atttccaaga aaggtgtggc tgagtttgag
     7501 gcctttacgc cagattacct catggaagaa atgggcctca caccagctca gtttatcgat
     7561 ctcaaggcgc tcatgggtga taagtcggat aatatccctg gggtgaccaa agtcggtgaa
     7621 aagacgggta ttaagctctt gctggagcat ggttcgcttg aggggattta tgaaaatatt
     7681 gatggaatga agacttctaa gatgaaggaa aatctcatca atgacaagga acaggccttt
     7741 ttgtctaaaa cactagcgac cattgatacc aaggcaccga ttgcgattgg tttagaggac
     7801 ttggtctata gtggtccaga tgttgaaaat cttgggaaat tttacgatga gatgggcttc
     7861 aaacagctca agcaggcttt aaatgtgtcg tcagctgatg tgtctgagag tttggatttt
     7921 actattgttg accaaatcag tcaagatatg ctgagtgaag agtctatctt ccactttgag
     7981 ctttttggtg agaattacca tacggataat ttggttggat ttgcctggtc ttgtggggat
     8041 aagctctatg ccacagacaa gcttgagctg ttgcaagacc cgattttcaa ggatttctta
     8101 gaaaaaacat ctctgagagt ttatgacttt aagaaggtta aagttctttt gcaacgtttt
     8161 ggtgtggatt tgcaggcgcc tgcttttgac atccgtttgg ctaaatacct cctttcgact
     8221 gtggaggaca atgaaattgc gaccatcgct agtctttatg gtcagactta cttggttgat
     8281 gatgaaactt tctacggtaa gggtgttaaa aaggccattc ctgaacgtga gaaattcttg
     8341 gaacatttag cttgtaaact tgctgttttg gtagaaacag agcctatttt acttgaaaaa
     8401 ctcagcgaaa atgggcaatt agagcttctt tatgatatgg agcaacctct ggcttttgtt
     8461 cttgccaaga tggaaattgc tgggattgtg gtcaagaaag agaccttgct tgagatgcag
     8521 gctgaaaatg agcttgtcat tgaaaaactg actcaagaga tttacgagct ggctggtgag
     8581 gagtttaatg tcaactcgcc taagcagttg ggcgtgcttc tctttgagaa attgggactt
     8641 cctctagaat acactaagaa aaccaagaca ggttattcga cagcagtgga tgtgttggag
     8701 cgtctcgctc ctattgctcc gattgttaag aaaatcctgg attaccgtca aattgctaag
     8761 attcaatcta cttatgtaat tggcttgcag gactggattt tggctgatgg aaagattcat
     8821 actcgctata tgcaggattt gacccagacc gggcgtttgt ctagtgtgga tccaaacttg
     8881 caaaatattc ctgcccgatt ggaacaaggg cgcttgattc ggaaggcttt tgtgccagag
     8941 tgggaggata gtgtgctact cagctctgac tattcacaga ttgaattgcg cgttttggcg
     9001 catatttcta aggatgagca cttgattaag gccttccaag agggggcaga tatccatact
     9061 tcgacagcca tgcgggtctt tggcattgag cgtcctgata atgtgactgc aaatgaccgt
     9121 cgcaatgcca aggcagttaa ctttggagtg gtttatggga tttcagactt tggcttgtct
     9181 aataatttgg gaattagtcg taaggaagcc aaagcctaca ttgataccta ctttgaacgt
     9241 tttccaggta ttaaaaacta catggatgaa gtggtgcggg aggcgcgtga taagggctat
     9301 gtagagaccc tctttaagcg tcgccgtgag ttgccagata tcaattcgcg caacttcaat
     9361 attcgtggtt ttgcggagcg aactgctatc aactcaccta tccagggttc ggcagcagat
     9421 attctcaaga ttgccatgat tcagctggat aaagccttgg ttgcaggtgg ttatcagact
     9481 aagatgctgt tacaagtgca cgatgaaatc gtccttgaag tgcctaaatc tgaattggta
     9541 gagatgaaaa aattggtgaa acaaaccatg gaagaagcca ttcaactcag tgttcctctt
     9601 atcgcagatg agaatgaagg ggcaacctgg tacgaggcta aataaaaagg gggctagtcc
     9661 tccttttttg tagtagaatt ctgcaaacct tttcaaaatg tgctatactg atgaaaaagg
     9721 aggatttcta tgagtcaaga agttatcaat ccaagtgatg gcgtgattcg tcagtatctc
     9781 gcaacgagta aaacccttgc tgtggtgggt ttgtctgacc gtgaagaaac aaccagcaat
     9841 cgagtgacaa aggaaatgca ggctcggggc tataaaatca tcccagtcaa tcccaaggcg
     9901 gcaggtggcg aaatcttggg tgaaaaggct tatgctagcc tcgctgaaat tccttttcct
     9961 gtagatattg tcaatgttta tcgtcgcagt gaatttctgc ccgatgtggc gcgtgatttt
    10021 ctcaaagctg atgccaagat tttttgggca cagctaggac ttgaaagttt agaagcgaaa
    10081 gagattttgc gtgatggtgg atgtgatgat atcgtgatga atcgctgtat caagagagaa
    10141 catactcgct tgattgagga agcataagaa aaaggtagct gatgggctac cttttgtgtt
    10201 atactcaatg aaaatcaaag agcacactag caaactagga aactagccgc gggctgctca
    10261 aaacaccgtt ttgaggttgt agatac
//
LOCUS       DBEROU010000059        10061 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.059, whole genome shotgun sequence.
ACCESSION   DBEROU010000059 DBEROU010000000
VERSION     DBEROU010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 10061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10061
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.059"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            201..1691
                     /gene="mgaSpn"
                     /locus_tag="ACL8CL_001918"
                     /gene_synonym="mgrA"
     CDS             201..1691
                     /gene="mgaSpn"
                     /locus_tag="ACL8CL_001918"
                     /gene_synonym="mgrA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor transcriptional regulator
                     MgaSpn"
                     /protein_id="HGS1331103.1"
                     /translation="MRDLLSKKSHRQLELLELLELLFEHKRWFHRSELAELLNCTERA
                     VKDDLSHVKSAFPDLIFHSSTNGIRIINTDDSDIEMVYHHFFKHSTHFSILEFIFFNE
                     GCQAESICKEFYISSSSLYRIISQINKVIKRQFQFEVSLTPVQIIGNERDIRYFFAQY
                     FSEKYYFLEWPFENFSSEPLSQLLELVYKETSFPMNLSTHRMLKLLLVTNLYRIKFGH
                     FMEIDKDSFNDQSLDFLMQAEGIEGVAQSFESEYNISLDEEVVCQLFVSYFQKMFFID
                     ESLFMKCVKKDSYVEKSYHLLSDFIDQISVKYQIEMENKDNLIWHLHNTAHLYRQELF
                     TEFILFDQKGNTIRNFQNIFPKFVSDIKKELSHYLETLEVCSSSMMVNHLSYTFITHT
                     KHLVINLLQNQPKLKVLVMSNFDQYHAKFVAETLSYYCSNNFELEVWTELELSKESLE
                     DSSYDIIISNFIIPPIENKRLIYSNNINTVSLIYLLNAMMFIRLDE"
     gene            2053..3048
                     /locus_tag="ACL8CL_001919"
     CDS             2053..3048
                     /locus_tag="ACL8CL_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1331104.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFWGDKTTKKI"
     gene            3175..4479
                     /locus_tag="ACL8CL_001920"
     CDS             3175..4479
                     /locus_tag="ACL8CL_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HGS1331105.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGGGYEDKVNAAITTNSLPDVITLDG
                     PNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYNKK
                     MFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYMPL
                     IWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYPML
                     LSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASEFV
                     KFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVAYP
                     QVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            4575..5477
                     /locus_tag="ACL8CL_001921"
     CDS             4575..5477
                     /locus_tag="ACL8CL_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1331106.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMISLVQRRVLKEDD"
     gene            5479..6324
                     /locus_tag="ACL8CL_001922"
     CDS             5479..6324
                     /locus_tag="ACL8CL_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1331107.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            6334..7653
                     /locus_tag="ACL8CL_001923"
     CDS             6334..7653
                     /locus_tag="ACL8CL_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HGS1331108.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKDLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWLMYTGHIEEKTGVRQVQNMAFSDDGIHFEKIEQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVAAKHKDNVGCIVLLGSDNLVEWQFESIFLKGGEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVDWGEKRFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDTDYLEFGYDSNVQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(7863..7935)
                     /locus_tag="ACL8CL_001924"
     tRNA            complement(7863..7935)
                     /locus_tag="ACL8CL_001924"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7900..7902),aa:Lys,seq:ctt)
     gene            complement(8200..9471)
                     /locus_tag="ACL8CL_001925"
     CDS             complement(8200..9471)
                     /locus_tag="ACL8CL_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HGS1331109.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFDFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWIKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            9741..9980
                     /locus_tag="ACL8CL_001926"
     CDS             9741..9980
                     /locus_tag="ACL8CL_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331110.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
BASE COUNT         3284 a         1567 c         1987 g         3223 t
ORIGIN      
        1 atctttagac ttgaatttga ttctgtattc acgccctcaa tagacggtaa aataagaaaa
       61 ttgtttatat attgtctccg tagtgttatt atacgaaata aaagatttta ggaaaagtcg
      121 ttatatcatg ctatacctat tctttgtggt ataattgcaa gaggtttaat ccgataattt
      181 ataaagagag aaagatattc atgagagatt tattatctaa aaaaagtcat aggcaattag
      241 aattattaga attattagaa ttattatttg aacataaacg ttggtttcat cgttctgaac
      301 tagcagagtt attaaattgt acagaacgtg cagtcaaaga tgatctatcc catgttaaat
      361 ctgcttttcc tgacttgatt tttcattctt ctactaatgg tatacgcatt attaataccg
      421 atgatagtga tattgaaatg gtttaccatc atttctttaa acattcaact catttttcga
      481 ttttagaatt catcttcttt aatgaaggtt gtcaagctga gagtatttgt aaagaatttt
      541 atatcagttc atcttcgctc tatcgtatta ttagccaaat caataaagtg attaaaaggc
      601 aatttcaatt tgaagtcagt ctgacccctg ttcaaatcat tggaaatgag agagacattc
      661 gttacttttt tgcacaatat ttttcagaaa aatattattt cctagaatgg ccatttgaaa
      721 atttttcatc agagccacta tctcaattgt tagaattggt ttataaggaa acaagctttc
      781 caatgaattt gtcaacgcat agaatgctaa agttgctcct agttacgaac ctatatagaa
      841 taaagtttgg tcattttatg gaaatagata aagattcttt taacgaccaa agtttggatt
      901 ttttaatgca ggcagaagga atagaaggtg ttgctcagag ttttgaatca gaatacaata
      961 tctctttaga tgaagaagtt gtttgccaat tatttgtgtc ttactttcaa aaaatgtttt
     1021 tcatagatga aagtctcttt atgaaatgcg taaaaaagga tagttatgtt gaaaaatctt
     1081 accatctatt gagtgatttt attgatcaga tttcagtcaa gtatcagatt gaaatggaga
     1141 ataaggataa tctgatttgg catctgcata ataccgcaca tctgtatcgt caagagttgt
     1201 ttactgagtt tattttattt gatcaaaaag ggaatacaat caggaacttt caaaatattt
     1261 ttccaaaatt tgtttcagat ataaaaaaag agctttctca ctatttagag actcttgagg
     1321 tttgttctag ttcaatgatg gtaaatcatc tatcttatac cttcatcact cataccaaac
     1381 atttggtgat taatttgcta caaaatcagc ctaaactaaa ggttttagtt atgagtaatt
     1441 ttgatcagta tcatgcaaaa ttcgttgcag agacactttc ttattactgt agcaataatt
     1501 ttgaactgga agtttggact gaattagaat tatcaaagga atctttagaa gattcatctt
     1561 atgatatcat tatttctaat tttattattc ctcctattga aaataagaga ctcatctatt
     1621 caaataatat aaacacggtc tcactcatat atttgttaaa tgccatgatg tttattcgat
     1681 tagatgagta accaaaaata acaaaaaaac gctatttcgc gtttgaactg tacctcaaaa
     1741 gttagacaga aaaaatctaa tttttggggt gtttttgtta agaaaagacc ttcaatcatt
     1801 taagcaaaat gtacatctag atttatagcc ctctaaaact cttgcttttt ttgtagaaga
     1861 tacgaaaaaa gccataaaac caaggctttt cctgttttat gtagatgttt acacatgaaa
     1921 attgaagtat tttcaaaagc taattggagt gattttattc atgatgggat aaatgatctg
     1981 gccaaaaatg attgagaaat agttgcttaa aaagcatttg catgatacta tatattgaaa
     2041 ggaggtgata atatggtaga acaaagaaaa tcaattacca tgaaagatgt tgctttagaa
     2101 gcaggagtta gtgttggaac tgtttcacgt gtaattaata aagaaaaagg cattaaagaa
     2161 gtaactttaa ggaaagttca aagggctata aaaacattgg actatatccc tgataattat
     2221 gctcgaggta tgaaaaagaa tagaacagaa actatcgctt tgattattcc gagcatatgg
     2281 catccatttt tttcggagtt tgcctatcat gttgagaaag agttattaaa atataattat
     2341 aaattattta tttgtaacgc tgatagcgat tcacaaaatg aaatcgaata tatcgaaatg
     2401 ttgaaacaaa ataaagtgga tggaattatt ggtattacat attctgatat tgacaaatat
     2461 attttatctg atttaccgtt tgtaagtata gatagacatt tttcggaaaa tgtatgctat
     2521 gttacttcag caaattacaa aggaggacaa actgcagcag aggaactggt tagaagggat
     2581 aataagcacc ttgcttttat tggttcaatt aatatgtatg agaatgagac tatactcaga
     2641 agtaaaggat tcagagaaaa agctaaggaa ttaggagtag atgtctataa ttttgaaatg
     2701 aaagaacctt taataaatcc tgaagatcag ataagaattt ttttgcaatc tcataaagaa
     2761 attgatggta tatttacaat aaatgatatg atggctctga gagttattag tgttatgaaa
     2821 gaactgggga aaatagcacc tgatgattac cagataattg ggtttgatgg tttaaaaact
     2881 tctgtcgatc aatcttatct agtgtctaca atagttcaag atattggtat gatggcaaaa
     2941 gaatctgttg caatgatact gaaaatgatt aagcgagaag agattctaga aaaacgaatc
     3001 gtaattccca catatttttg gggagataaa acaacaaaaa aaatttaaaa agcgcttgac
     3061 aattaatgaa agcgctgtta taatataatc aacaaatgaa acgtttcaaa aataggctag
     3121 aagcttgttt aaaaaataaa taaaaaatga aacgtttcaa aaaggaggaa aatgatgcgt
     3181 aaaggtacaa tgttatctgt tgttgcgggg ctatcgttag ctatacttgc aggttgtagt
     3241 ggaggaacta actcaaaaca agcgagttct tcaaatgata ttaaagtctg ggtacaattc
     3301 tctgacgaaa caaatgaagg aaaagcttgg caaaaagttg ttgataactt taatcaatca
     3361 ggaaaaagta agtacaaggt tgtaactgag tatattcctc gtagtggtgg aggatatgaa
     3421 gacaaggtta atgctgctat tacgactaat agtcttccag atgttattac ccttgatgga
     3481 ccaaatacgg cagcttatgc aaaatctaaa gtaattactc cgctagatga ttatctaaaa
     3541 gataataata tggatgatgt tttggacagt attaaacaac agggaactta taatgggaaa
     3601 ttctatgcat ttggattttc tgaatcgaat gttggtgttt attacaataa aaaaatgttc
     3661 aaagaggccg gaattgcgga atcagagctt ccaacgctag aaaagccatg gacatgggac
     3721 gaatttaata cgattgctaa aaaattgaaa gatcattata acaaaccagc aattgatttt
     3781 cgtattaatt caaacgatga aatgttgcca tatgcttata tgccgttgat ttggtcaaat
     3841 aatggttctg tcgtaaacga agatggaaca aaggcagaag gatacttcaa ctccaaagag
     3901 agtgtagaag cagttcaatt cattcaaaat cttgtaaaag aaggctatac aactgttagt
     3961 ccagtcgaaa aaggttttga aacaggagag tatccaatgc ttttgagtgg ttcttggacg
     4021 attgctgaca tgaatacaaa ctacaaagat attgattttg gtatccttcc ttacccagta
     4081 tctagtaaga cgaaaaaatt agtatcccct tcaggtagtt ggcaattagc tgtgacgaca
     4141 aaatctgata aaaaagaggc cgcatctgaa tttgttaaat ttgctacaaa tacagaatca
     4201 agtgaaatta ttagcttggg gaattcggtc cttccaattc gtaaatctac aattgaaaat
     4261 atcaaagata aagtatccga accaatgcgt ttcttgatgg aacaaaattc aaaaacagct
     4321 catgctcgcc cagttgttgt agcatatcct caggtttctc gagcttttca acaagcaatg
     4381 caagatatta gctattatga agaaaatcca aatgtgcaaa aagtcttgga tactcgtaca
     4441 aaagaaatgc aaactgctat tgatcagtca ctcaaataat ctgtaaaaaa gaaggtggaa
     4501 ggaaaatgtt tggaactacc ttcccctttc ttgttaatag cctagtttga tatatatgat
     4561 taaaggagat ggatatgagt aaagaaactg ttgtggatac aaaaagacgt gaaaaaataa
     4621 aggataacat attaggttat agttttttag ctccagcttt aattttatta ggtatctttt
     4681 tagtaattcc agttggaatg gtcatttatt acgcttttac agattattat ttgttgacac
     4741 cagatgaaag aaaatttgtt ggacttgaaa attttattcg attaacaaaa gatccgattt
     4801 tcttaaagag cttcttgaat actttgaaat ttgttgtttg gattattcca gttcaattgg
     4861 gagcggcctt aggaatggct ttgattgtca ataaaaagcg aaaaggaaat atgtttttta
     4921 aagtggcttt ttttgctcca gttgtcatgt ccttggtagt tatttctatt ctttggcttt
     4981 atcttttaaa tccaaatagt ggcttattaa atgctatttt aaataaggta ggtattgctt
     5041 ctcaaccttt cttgactagt cctaaacaag ctatgtatgc aattgttttt gtttctgctt
     5101 ggcagggtgc aggttatcag atgcttttat ttttaggagg aatgcagaat attccacaag
     5161 atgtctatga agctgctgaa ttagatggat tctcaaaatg ggctcagttt cgttatatca
     5221 cgatgcccct actaaaacca acagccctgt ttgtattgtt aacaacttta atttctgctt
     5281 ttaagctgat tgtacaacca atggttatga cgcaaggtgg gccattaaac tcaacaatta
     5341 ctatggtgta ctatatttat caacaaggtt ttactgatcg tcttgtagga tattctagct
     5401 caatcgcttt agtatttaca acgttaattg gtatgattag cttagtacaa cgtcgcgttc
     5461 tgaaggagga tgactaagat gaaacgattt aaaccaacaa caatcttaga atatattctg
     5521 ttaactttat tggcaggact cttccttttt cctatggttt ggatggtagt ttcagctatg
     5581 aagccagaag cagatgttta taaaaatttg actagttggc aagccttctt accaagttta
     5641 aatcccgctg attggtttaa accttatcag gaagtatttg agcgctttag tatttttaca
     5701 tatctaggga acagcatttt ttatgctgga acgtttgcta ttggttctat tattgtcaat
     5761 gcactggccg gtttcgcttt tgcaaaagtc aatttttcag gtaagaaaat tttatttggt
     5821 ttcttacttg ctttacttat tattccagtg gaaactgtgt taattccaca atttaccatt
     5881 atcaattcct taggtttggt caataatcgt cttgcagtta ttattccagg attggcgagt
     5941 gtatttaata tttacttatt taggaatttc tttattgcaa ttccagaaga gattcttgag
     6001 tctgcaaaga tggacggagc aagtatcgtt agaattttct ttagaattat gcttccaatg
     6061 tctaaacctg cagttgctac agttggtgtt ttatcattta tttcaagctg gaatgattat
     6121 atctggccat tgatggtttt gacagattct tctaaatttt caatgcaggt tgcaattaca
     6181 actattaata caactcaacc agtttatatc aatcaggtaa tggctgtatt aactatttca
     6241 acgattccgt tgattttggt ctatgtagtc gctcagaaat atattgttca aggtctgggt
     6301 ggttccggaa ctggaataaa ataggatata cttatgataa ataaaacatt tacagtagaa
     6361 aaagccaatc gttttatagc agaaaataaa catctcgtta atactcaata taagcctgaa
     6421 gaacattttt cagctgagat tggttggatc aatgatccaa atggatttgt ttattttcgt
     6481 ggagaatacc atctctttta tcaattctat ccatatgata gtgtttgggg acctatgcac
     6541 tggggacatg ctaaaagtaa ggacttggtg acttgggagc acttgccagt ggcacttgct
     6601 cctgatcaag attatgaccg aaatggttgt ttctcaggct ctgccattgt caaggatgat
     6661 cgcctctggc tcatgtacac tgggcatatc gaagaaaaaa ccggtgtccg ccaagtgcaa
     6721 aatatggcat tttcagacga cgggattcac tttgaaaaga ttgaacaaaa tccagttgca
     6781 acaggagcag acttgccaga tgagttgatt gctgctgatt tccgtgatcc aaaactcttt
     6841 gaaaaagatg gacgctatta ctccgtagta gctgccaaac acaaggataa tgtgggctgt
     6901 atcgttctac tagggtccga taacctagta gaatggcagt tcgaatccat ctttttaaaa
     6961 gggggagaac accaaggctt tatgtgggaa tgcccagatt acttcgagtt agatgggaaa
     7021 gattgcctta ttatgtcacc catgcgttat cagcgtgagg gagactcata tcataacatc
     7081 aactcatcgc ttttgttcac gggtaaggta gattggggag aaaaacgttt tatcccagaa
     7141 tcagttcaag aaattgatca tggccaagac ttctatgcgc ctcaaacatt gttggacgat
     7201 caaaatcgtc gtatcctgat tgcttggatg cagacatggg ggcgtaccct tccaactcat
     7261 gaccaagaac acaagtgggc atgtgccatg actttaccta gaattctaag attggaagat
     7321 ggcaaactaa gacaattccc tgttaaaaaa ggccaatatc aaatccaaat agataaagat
     7381 tgtcattacc acttaggaaa tgatacagat tatcttgaat ttggttatga cagtaatgtg
     7441 cagcaagttt acattgatcg tagccatctt attcaaaaaa ttctaggtga agaagaacag
     7501 gacactagtc gacggtatgt agatattgaa gctaaagaat tggaagttgt tctagataaa
     7561 aattccatcg agatttttgt caatcaaggt gaagcaagct tgactgcaac ttattactta
     7621 acggtgccag ctgagctatc acgaattgat taaaaattaa gttatttctc ctaaagaaaa
     7681 agttctcttt ctaaaatagt ggaaagagga ctttttgtgt tttgggtata taagcttagt
     7741 ttatggtatt tgtaaaattg gtgttggatt atgatttaaa ctagttttct aaagaatttg
     7801 aaaaaaattt tatttaagca aaaaaagccc tatattataa gacttttcct gttgtattta
     7861 tatgccccct acagggatcg aacctgtgac ccacggatta agagtccgct gctctgccag
     7921 ctgagctaag gaggcaaaga aaaaagctgt attggtgccg aaacttcacg gtttgtattg
     7981 aacccgcgca attaagcagg tgggcaactc gctctaactg aagctgtttc cgtgtgagac
     8041 ggcctacatg ctgttagaag acttttgttt ccctaataat acaaaaaata gtcggtcaac
     8101 acttaagtgt gaagtcgtac accacagcgt ttctatactt atatgatacc actttttcaa
     8161 aaaaaatcaa gaggaaagtg caattttttg aaaaggattt cagatttttc gcaaacgatc
     8221 gatagcttcc tttctttgag ccaaagcccg ttcatattta ccagtatctt ctgcttggaa
     8281 atagtgatga ttacgaattt tttctggcag atagtcttgc ttgatccaat ttccaggata
     8341 gttgtgtgga tagagatagt cttgggcatt ccccagttcc ttgcttccac tgtagtgccc
     8401 atcacgcagg tgtcgcggaa taggcaagtg ccctgatgtt ttgaggtcag caagtgcctt
     8461 atccatagct acataggctg agttggattt tggagaaagg gccaaatcaa tcacgacatt
     8521 ggcaatgaga atgcgggctt ctgggaaccc aatcttctgg gcagcatcca gagcagtcac
     8581 ggtatgaatc tgggcctcag gattggctaa gccgatatct tcataggcga taacagtcaa
     8641 gcgacgagcg agactaggca gatccccagc ctcaatcaag cgggcagtat agtggagact
     8701 ggcatccaca tctgagccac gaatagactt ttgcagggca gatagaacat catagtgacc
     8761 gtctccatcc ttgtccatag tgatgtagct tctctgaaga ctattttcca tgatgtctaa
     8821 ggtgatgtgg cgaatgccct cgtcattctc aggggtagag agaacagcca agtccagtga
     8881 gttaaaggca gagcgaaggt ctccgtttgt agaggttgcg atgaaatcca gcgcatcctc
     8941 atctagttct attggaaaat caaaaccacg ttcagggtta cttagagcta tctgcagggc
     9001 ctctttgacg tcttggttag acagaggttc caactcgaaa atttgaacac ggctacgaat
     9061 ggcaggagtg acagagaaga aaggattttc agtcgttgct ccaatcatga tgaccagtcc
     9121 actttccaag agaggcaaga ggaagtcttg cttggtctta tctagtcgat gaatttcgtc
     9181 tagcaatagg acgagaccac cagaaaattt agcttcttcc gagatttctt gcagtcgctt
     9241 tttactatca actgtcgcat tgaaagttcg aaaggcatac ttggtcgttc cagcgatggc
     9301 agaggcaata ctggttttgc cgattcccgg agggccatat agaatcatgg aggacaggcg
     9361 gttggcttcc accatgcggc ggatgatttt tccaggtccg accagatgct cctgaccgat
     9421 gacctggtcg atggttttag ggcgcatgcg aagcgcgaga ttgtctggca tagcagtcct
     9481 ttctaacgtg gatttgagta gaatgactca tctactatat cttactatgt ccattctagc
     9541 ctattagttg tgatatttca atggttttgt aataatcagg gagtgagata gaatcttttt
     9601 tactatttaa tttaatgagg agaggtatca gcatgagaga tttgattttc cttaaagctc
     9661 aaaataggat agttgagcct aaagaagtgg aagtgctgac ttgagaaata atattgctga
     9721 atgtcattgt atgaactata atgaaaagag aggtgataag caatggaaat gatggaccaa
     9781 gtcaggaaat tttgattttt acaaaacaaa ttcgtcattg gattcttagt gatcaagtta
     9841 tttcagggaa aagaaaactt ttcttccggg aagatactcc aaaagagatt ttagatttgt
     9901 acgaaaacat taaatctaaa cttgattttg cttaccaaga agttcattct aataatgggt
     9961 tgaaaaaata tgaaaaatag aataattgat gttttgaagt agtaaatcgc ctccttgtac
    10021 taactgttga aaatcctgat ttcgaagact tgagagttaa t
//
LOCUS       DBEROU010000060         9915 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.060, whole genome shotgun sequence.
ACCESSION   DBEROU010000060 DBEROU010000000
VERSION     DBEROU010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9915
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.060"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..736
                     /locus_tag="ACL8CL_001927"
     CDS             <1..736
                     /locus_tag="ACL8CL_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat-containing
                     protein"
                     /protein_id="HGS1331111.1"
                     /translation="KDSIDVPAAYLEKAKGEGPFTAGVNHVIPYELFAGDGMLTRLLL
                     KASDKAPWSDNGDAKNPALSPLGENVKTKGQYFYQVALDGNVAGKEKQALIDQFRANG
                     TQTYSATVNVYGNKDGKPDLDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPM
                     NHNHATGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSM
                     ASIGFLGLALAGLLGGLGLKNKKEEN"
     gene            903..1601
                     /locus_tag="ACL8CL_001928"
     CDS             903..1601
                     /locus_tag="ACL8CL_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HGS1331112.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            1598..2650
                     /locus_tag="ACL8CL_001929"
     CDS             1598..2650
                     /locus_tag="ACL8CL_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS1331113.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            2845..3456
                     /gene="rpsD"
                     /locus_tag="ACL8CL_001930"
     CDS             2845..3456
                     /gene="rpsD"
                     /locus_tag="ACL8CL_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HGS1331114.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(3550..4716)
                     /locus_tag="ACL8CL_001931"
     CDS             complement(3550..4716)
                     /locus_tag="ACL8CL_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HGS1331115.1"
                     /translation="MQIKQITKKDGSIVYRANVYLGVDKVTGKDVKTSITGRTKKEVK
                     QKTKEAEIAFLQNGSTRFQASNITTYKELASLWWESYKHTVKPNTQLNVRRLLDNHIL
                     PLFGSYKLDKLTTPLIQNIVNKLADKTNKGEEGAFLYYDSLHALNKRILQYGVVMQAI
                     PFNSAREVILPRNTQKAKREKVKHFDNDELKSFLKYLDTLDLSRYRYYYEITLYKFLL
                     ATGCRINEALALSWSDIDLDNAIVHITKTLNYNQELNSPKSKSSYRDIDIDQATVSML
                     KQYKHRQVQEAWQLGRSETVVFSDFINEYPNNRTLQTRLRTHFKRADVPNIDFHDFRH
                     THASLLLNSGIPYKELQHRLGHSQLSMTMDIYSHLSKENAKKAVSFYETALKSL"
     gene            complement(4878..4982)
                     /locus_tag="ACL8CL_001932"
                     /pseudo
     CDS             complement(4878..4982)
                     /locus_tag="ACL8CL_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505376.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            4985..5167
                     /locus_tag="ACL8CL_001933"
     CDS             4985..5167
                     /locus_tag="ACL8CL_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000896637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331116.1"
                     /translation="MDPDKRQKIFSQCKKEGDSLEQRKQEILEKYANKQDKPKSRKND
                     SKGSESHKRKTKSKEF"
     gene            5217..6077
                     /locus_tag="ACL8CL_001934"
     CDS             5217..6077
                     /locus_tag="ACL8CL_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HGS1331117.1"
                     /translation="MTTNLVKQKENLEAYIRSTGYNTRGMNVENNHVLIEKPILDSYE
                     DEHQRKELVDLVNVIETRTRGGKYEVTDFESDSLQEISENFVERTESDKKKTISVDYL
                     VKLFSGKLDFSQEQLDDGQYNLTDFLGKKIIKLKRRTRNREIGKILQTAKVQTATSLD
                     DLKSIVSLINPERNVSIVISQSLFSVLEKMKDTSGNYLLKVDKETGTSETFFVDNFLI
                     VDDTTLGNEGDKKGFIGDLENFVTLFDRKKDTLSWVNANDYFRKRLILHTRFDVKKVE
                     EDCGYFIQWN"
     gene            6087..6635
                     /locus_tag="ACL8CL_001935"
     CDS             6087..6635
                     /locus_tag="ACL8CL_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331118.1"
                     /translation="MDINQVFDTLDDLDNKKSKIYSAREQLSEKRKSLLGIQTVSFEN
                     INSFLSNNLESLEKLEKMEKAINSLQEKYNSDFSEAKAVIFEYIFKETKQRMETKKIY
                     KQYRKKLRRILDAYDEIQELKKDVEEIHTGVVREISQKHSLSLYRTEVSPLTVLPFLN
                     PDISGWMDFSKEYRDIKEYLEK"
     gene            7013..7423
                     /locus_tag="ACL8CL_001936"
     CDS             7013..7423
                     /locus_tag="ACL8CL_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HGS1331119.1"
                     /translation="MYELSNRDLDGIDIELGRYRTLASKIYLRRQELIHNKKHSTEDY
                     TGGKGKTVYSPTEATIIRIEEDQTLRYLEGFKLIVDTLMENLIDTDLVIFKMRFLEAG
                     VTWEDVAEKLNKTTRYINSRRKVIAKRFIELKGY"
     gene            7799..8992
                     /locus_tag="ACL8CL_001937"
     CDS             7799..8992
                     /locus_tag="ACL8CL_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331120.1"
                     /translation="MTAEIGILNKNGVVLAADSAVTLSDGINSKVFNSARKLFTLSRV
                     HSVGIMIYGDASFMGVPWEVIISEYKKSIGNEVLADTAMYINNFVDFLLQFMPIQSND
                     ALVNYIIRQTRSYLKIIHESTQEIADHRSDQGDVITLEIFKNILWEVIEEYSNDISKS
                     ISETEFEYLDGELDLIREQLDEYFNNNEYSDEQLNFLSKTIYKAMLVGFDRNSVTGLV
                     IAGYGVEEIFPSLRQIELSGVFAKRLVWRVITEKEISQNVACHIIPFAQSEMVDTIMN
                     GIDPMLNAFIGQQINEILESNNISDAQEELFETIARVQQQYYINPIFELIGMQPIDEM
                     ASTAKTFIELTSFKRKIVNTLETVGGPVDVLAISKGEGPIWIERKHYFNIENNIDYKI
                     RKGDI"
     gene            8992..9159
                     /locus_tag="ACL8CL_001938"
     CDS             8992..9159
                     /locus_tag="ACL8CL_001938"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331121.1"
                     /translation="MYAANFSKEKYGFLDKKIYQNLSQSNVGKNFLEVYKEIDTKPKN
                     QISKTLVDKSI"
BASE COUNT         3429 a         1538 c         1998 g         2950 t
ORIGIN      
        1 taaggacagt atcgatgttc cagcagccta cctagaaaag gccaagggtg aaggtccatt
       61 cacagcaggt gtcaaccatg tgattccata cgaactcttc gcaggtgatg gtatgttgac
      121 tcgtctcttg ctcaaggcat ctgacaaggc accatggtca gataacggtg acgctaaaaa
      181 cccagcccta tctccactag gtgaaaacgt gaagaccaaa ggtcaatact tctatcaagt
      241 agccttggac ggaaatgtag ctggcaaaga aaaacaagcg ctcattgacc agttccgagc
      301 aaacggtact caaacttaca gcgctacagt caatgtctat ggtaacaaag acggtaaacc
      361 agacttggac aacatcgtag caactaaaaa agtcactatt aagataaatg ttaaagaaac
      421 atcagacaca gcaaatggtt cattatcacc ttctaactct ggttctggcg tgactccgat
      481 gaatcacaat catgctacag gtactacaga tagcatgcct gctgacacca tgacaagttc
      541 taccaacacg atggcaggtg aaaacatggc tgcttctgct aacaagatgt ctgatacgat
      601 gatgtcagag gataaagcta tgctaccaaa tactggtgag actcaaacat caatggcaag
      661 tattggtttc cttgggcttg cgcttgcagg tttactcggt ggtctaggtt tgaaaaacaa
      721 aaaagaagaa aactaatcag ctaaggaaat aaatgatgga tagtgggctg actaagatta
      781 gtttaacaac tcaatcagca atcaggactt tctttcaata gcagattaaa atcatcgtaa
      841 aacaataaaa atagtgttat acttaaagca gtatagcact gtttttatca aaggagagac
      901 agatgggaaa gacaatttta ctcgttgacg acgaggtaga aatcacagat attcatcaga
      961 gatacttaat tcaggcaggt tatcaggtct tggtagccca tgatggactg gaagcgctag
     1021 agctgttcaa gaaaaaaccg attgatttga ttatcacaga tgtcatgatg cctcggatgg
     1081 atggttatga tttaatcagt gaggttcaat acttatcacc agagcagcct ttcctattta
     1141 ttactgctaa gaccagtgaa caggacaaga tttacggcct gagcttggga gcagatgatt
     1201 ttattgctaa gccttttagc ccacgtgagc tggttttgcg tgtccacaat attttgcgcc
     1261 gccttcatcg tgggggcgaa acagagctga tttcccttgg caatctaaaa atgaatcata
     1321 gtagtcatga agttcaaata ggagaagaaa tgctggattt aactgttaaa tcatttgaat
     1381 tgctgtggat tttagctagc aatccagagc gagttttctc caagacagac ctctatgaaa
     1441 agatctggaa agaagactac gtggatgaca ccaatacctt gaatgtgcat atccatgctc
     1501 ttcgacagga gctggcaaaa tatagtagtg accaaactcc cactattaag acagtttggg
     1561 ggttgggata taagatagag aaaccgagag gacaaacatg aaactaaaaa gttatatttt
     1621 ggttggatat attatttcaa ccctcttaac cattttggtt gttttttggg ctgttcaaaa
     1681 aatgctgatt gcgaaaggcg agatttactt tttgcttggg atgaccatcg ttgccagcct
     1741 tgtcggtgct gggattagtc tctttctcct attgccagtc tttacgtcgt tgggcaaact
     1801 caaggagcat gccaagcggg tagcggccaa ggattttcct tcaaatttgg aggttcaagg
     1861 tcctgtagaa tttcagcaat tagggcaaac ttttaatgag atgtcccatg atttgcaggt
     1921 aagctttgat tccttggaag aaagcgaacg agaaaagggc ttgatgattg cccagttgtc
     1981 gcatgatatt aagactccta tcacttcgat ccaagcgacg gtagaaggga ttttggatgg
     2041 gattatcaag gagtcggagc aagctcatta tctagcaacc attggacgcc agacggagag
     2101 gctcaataaa ctggttgagg agttgaattt tttgacccta aacacagcta gaaatcaggt
     2161 ggaaactacc agtaaagaca gtatttttct ggacaagctc ttaattgagt gcatgagtga
     2221 atttcagttt ttgattgagc aggagagaag agatgtccac ttgcaggtaa tcccagagtc
     2281 tgcccggatt gagggagatt atgctaagct ttctcgtatc ttggtgaatc tggtcgataa
     2341 cgcttttaaa tattctgctc caggaaccaa gctggaagtg gtggctaagc tggagaagga
     2401 ccagctttca atcagtgtga ctgatgaagg gcagggtatt gccccagagg atttggaaaa
     2461 tattttcaaa cgcctttatc gtgtcgaaac ttcgcgtaac atgaagacag gtggtcatgg
     2521 attaggactt gcgattgcgc gtgaattggc ccatcaattg ggtggggaaa tcacagtcag
     2581 cagccagtac ggtctaggaa gtacctttac cctcgttctc aacctctctg gtagtgaaaa
     2641 taaagcctaa aaccccttta caaatccagc tattcatggt agaatagatt ttgtgtgaaa
     2701 tatcagcagg aaagcatgaa gctcgtcaac aggtgtctta tgacaagtaa ccttggctgt
     2761 ttaggcgaag ggcatctgca cgaatcaggg ctttctaagt gactatttcc accgaaatat
     2821 tatttatatc aggaggacat tcatatgtca cgttatacag gaccatcttg gaaacaagct
     2881 cgtcgccttg gcctttcact tacaggtaca ggtaaagaat tggcacgtcg taactacgta
     2941 ccaggacaac acggaccaaa caaccgttct aaattgtcag aatacggttt gcaattggct
     3001 gaaaaacaaa aacttcgttt cacttacggt gtaggtgaaa aacaattccg taacttgttc
     3061 gtacaagcta caaaaatcaa aggcggaatc ctaggtttca actttatgct tcttttggaa
     3121 cgtcgtttgg ataacgttgt ttaccgtctt ggtctcgcga ctactcgtcg tcaagctcgt
     3181 caattcgtaa accacggtca catccttgtt gacgggaaac gcgttgatat cccatcatac
     3241 cgcgtaactc caggtcaagt gatctcagtt cgtgaaaaat cattgaaagt tccagcaatc
     3301 cttgaagcag tagaagctac tcttggacgt ccagcattcg tatcattcga cgctgaaaaa
     3361 ttggaaggtt cattgactcg cttgccagaa cgcgacgaaa tcaacccaga aatcaacgaa
     3421 gcacttgtcg ttgaattcta caacaaaatg ctttaatttt aagaaatatc ttacagaaag
     3481 cctacaacag tgggcttttt gctttgtctt aaaatgttga tttctgggtt tgttcagtta
     3541 tttgttcagt tataaacttt ttagtgcagt ttcatagaac gaaactgctt tttttgcatt
     3601 ctcttttgag aggtgactgt atatatccat ggtcatagat aattgggaat gtcctagacg
     3661 gtgctgaagt tccttgtaag gtattccaga gttaagcaag agactagcgt gagtatggcg
     3721 aaaatcatga aaatcaatgt taggtacgtc agcacgttta aagtgagttc ttaacctggt
     3781 ttgtagagtt cgattattgg ggtactcatt tatgaagtca gaaaagacta cagtttcaga
     3841 gcgcccaagt tgccaagctt cttgtacttg tctatgtttg tactgcttca gcatactaac
     3901 agtcgcttga tctatatcga tatcacgata gctagatttt gatttaggac tattgagttc
     3961 ttgattatag tttagtgttt ttgtgatatg cacaatagca ttatctaagt caatatcaga
     4021 ccatgataag gctaaagctt cattgatacg acaaccagta gcaagtagaa acttataaag
     4081 tgtaatctca taataatagc gatacctact tagatccaga gtatctaagt atttaagaaa
     4141 gctttttaat tcgtcattat caaagtgctt tactttttct cgctttgctt tttgtgtgtt
     4201 gcgaggtaat attacctcac gagcagaatt aaacggtata gcttgcataa ctacaccata
     4261 ctgtaaaata cgcttattta aagcgtgtaa gctatcgtag tatagaaaag cgccttcttc
     4321 ccctttattg gttttgtcag caagtttatt gactatattt tgaataagtg gagtagttag
     4381 tttatccaac ttataagagc caaataaagg taagatatgg ttatctagca gtcttctaac
     4441 gtttaattgt gtgttaggtt ttactgtatg cttgtaactt tcccaccata aggaagctaa
     4501 ttccttgtaa gtagtaatat ttgaagcctg gaatctagta gatccatttt gtaaaaaagc
     4561 aatttcagct tcttttgtct tttgcttaac ttcttttttt gtccttcctg ttatgcttgt
     4621 ctttacatct tttccagtga ctttatcaac accaagatag acattagcac gatatactat
     4681 tgaaccgtct ttttttgtga tttgttttat ttgcatagta ttcttttcca tcagcaggca
     4741 agcaattaga aaaggttttg agtttatacc atgctaggag ctacgagaac ccctctattt
     4801 tcgattttaa gagaacgaac ggtaaaatgt accaggtaag aaaatgaagc gattatggaa
     4861 cttataaagg gtattaatta gataatcttc tcaaatacca ttgtggcttg aatgcggtca
     4921 ccaccgccta aacctttgct tccaccattg gcggttgtga tggtatgcag gtgataagtt
     4981 ttctatggat ccagataaaa gacagaaaat cttttctcag tgtaaaaaag aaggtgatag
     5041 cttagaacaa cggaaacaag aaatactaga gaaatatgct aacaaacaag acaaaccaaa
     5101 atctagaaaa aatgattcta aaggctcgga gagtcataaa agaaaaacta agagcaaaga
     5161 attttagaaa aaattataaa caaaaatcag atattaaaag atgaaggagc aaaaaaatga
     5221 caactaactt agttaaacaa aaagaaaatt tagaagctta tatccgaagt acaggttata
     5281 acactagagg gatgaatgta gaaaataatc atgtactcat tgaaaaacca atccttgata
     5341 gttacgaaga tgaacatcaa cgtaaagaac tggttgatct agtaaatgtt attgagactc
     5401 gtacccgtgg cgggaagtat gaggtaactg actttgaatc tgattcatta caagaaatta
     5461 gtgaaaattt tgttgagaga acagaatcag ataaaaagaa aactatcagc gttgattact
     5521 tagttaaatt attcagtgga aaacttgatt tttcacagga acaattagat gatggccaat
     5581 ataatttaac ggattttctt ggtaagaaga ttattaaatt aaaacgtaga acacgaaata
     5641 gagagattgg gaaaattctc caaactgcga aagtacagac cgctacaagt ctggacgact
     5701 tgaaatctat tgtttcttta atcaatccag agcgcaatgt atctatagtt attagtcaat
     5761 cactatttag tgtcttagaa aaaatgaaag acacttcagg aaattatctt cttaaagttg
     5821 ataaagagac aggaacgagt gaaacattct ttgtagataa ctttttaatt gtagatgata
     5881 cgacattagg gaacgaaggc gacaaaaaag gatttatcgg agatctagaa aactttgtta
     5941 ctttgtttga tcgaaagaaa gatacactta gttgggtgaa tgcgaatgac tattttagaa
     6001 aacggttgat tttacatacc cgatttgatg taaaaaaagt tgaagaagat tgtggttact
     6061 ttattcaatg gaactaggag gaagaaatgg atattaatca agtatttgat acactggatg
     6121 atttagataa taaaaaaagt aagatttatt cagcacgaga acagttaagc gaaaaaagga
     6181 agagtctttt aggcattcaa acagtttcat ttgagaatat aaattctttt ttgtcaaata
     6241 acttagaatc tttagaaaag ctggaaaaga tggaaaaagc tattaattct cttcaggaaa
     6301 aatataatag tgatttttca gaagctaaag cagtcatctt tgaatacatt tttaaagaaa
     6361 ctaagcaacg gatggaaact aagaagatct ataaacaata ccgaaagaaa cttagacgaa
     6421 ttctggacgc atatgatgaa attcaagaac taaagaagga tgtagaagaa attcatacag
     6481 gtgtagtcag ggaaataagt cagaaacatt ctctatcgct atatcgaacg gaagtaagtc
     6541 cgcttactgt cctaccattc ttaaaccctg atattagcgg gtggatggat ttttctaagg
     6601 aatatcggga cattaaagag tatttagaaa aatagggaac aaattaagta aggctagtga
     6661 tatatggctg aaacagaaga gatatcgcta gtcctacttt tatgctttac taagtttcac
     6721 ataacaaagt aagcataaac taaaatagag tataggcttg gaagtcatat atatcagtaa
     6781 gttacagaat ggagtgagtt tcacagaatg taagatatga gaaacttagg ggataaatta
     6841 aagaaatttc ccttgaactt gtcatattga agagttgtca aacttaaaac aatgatacct
     6901 ggtaagtgga gtgttggaag gcttttagcg ctttttgtca gtttgacaga atttacaatt
     6961 tgacaaattg aaagataaaa aaatttttaa atttaagtgg aggtacttgc ctatgtacga
     7021 gttgagtaac agagacctgg acgggataga tattgagtta ggacgatata gaacgcttgc
     7081 tagtaaaatt tatttaagaa gacaggaact gatacataat aagaaacata gcactgaaga
     7141 ttatactggt ggaaaaggca agacagtata tagtcctacc gaagcaacta ttattagaat
     7201 tgaagaagac caaacactaa gatatttaga aggtttcaaa ctaattgtag ataccttgat
     7261 ggaaaatctg attgatactg atctagtcat ctttaaaatg agatttttag aagctggtgt
     7321 gacttgggaa gatgtggcag agaaactaaa taaaactact cgttatataa atagtcgaag
     7381 aaaagtaatc gctaaaagat ttatagaact gaaaggatat tgactccccc cacgttgaaa
     7441 aaaatttttt gaatactttg agaaccggtg aagggaactt tttctaagtc ggagacctcc
     7501 agacaaaaag ggggtaaaaa cttgttgatt tataaggtga aggctagttt tttgaaatta
     7561 aacatatgca ttcaaaagtc attgaaataa atgaggaggt taaactgaag ataatttttc
     7621 tagattaaag tgaagctatg atatattgtt agaaattgga gatgtgtttg catagttaaa
     7681 agaatgtact tttagtatga attgtattat aattagttaa agggaattgt tgtataaatt
     7741 gaaaaatatt taatgatttt taactgttaa gttatgtaaa ataattggag gctcaatttt
     7801 gactgcagaa attggaatac taaataagaa tggagttgtt ttagctgcag atagcgcggt
     7861 gactttatct gacgggatta actccaaagt gtttaatagt gctcggaaat tatttacttt
     7921 atctagagtg cattcggttg gaattatgat ttatggagat gcctccttta tgggtgtacc
     7981 gtgggaagta atcataagtg agtataaaaa gtcaattggc aacgaagtac tagctgacac
     8041 agcaatgtac attaataatt ttgttgattt tttattgcaa tttatgccaa tacaaagtaa
     8101 tgatgccttg gttaattata taattagaca gactagaagt tatttaaaaa taatacacga
     8161 gagcacccaa gaaatagctg atcatagatc agatcaggga gatgtaataa ctctagaaat
     8221 ttttaaaaat attttatggg aagttataga agaatattca aatgatatct ccaaatcaat
     8281 atctgaaact gaatttgaat atttagatgg cgaacttgat ttaatacggg aacaactaga
     8341 tgagtacttt aacaataatg aatactccga tgagcaacta aactttttat ctaaaacaat
     8401 atacaaagca atgttagttg gttttgatag aaattctgta acagggttgg taattgctgg
     8461 gtatggtgtt gaagaaattt ttccatccct tagacaaata gagttaagtg gtgtatttgc
     8521 taaacgcctt gtctggcgtg taattaccga gaaagaaatt agccaaaatg ttgcttgtca
     8581 tattattccc tttgctcaat ccgaaatggt agatactata atgaatggca tagatccaat
     8641 gttaaacgca tttataggac aacaaattaa tgaaattttg gaaagtaata atatatcgga
     8701 tgcacaggaa gagttgtttg aaactatcgc tagagtccaa caacaatatt atataaatcc
     8761 aatttttgag ttgattggga tgcaacctat tgatgagatg gcttctactg caaaaacatt
     8821 cattgagttg acttctttta agagaaagat tgtgaataca cttgaaacag ttggaggacc
     8881 agtggatgtt ttagctattt ctaaagggga aggtcctatc tggattgaaa gaaaacacta
     8941 ctttaatatt gaaaataata ttgattataa aattagaaag ggtgatatat aatgtatgca
     9001 gcaaattttt caaaagaaaa atatggattt ctagataaaa aaatatatca gaatctttca
     9061 caaagtaatg ttgggaaaaa ttttttagaa gtatataaag agatagacac taaaccaaaa
     9121 aatcaaatca gtaaaactct tgttgacaag tctatctaaa atgaaaacac ttagattgtt
     9181 ctaagtgttt ttgcttaaat atgattgtta ctatgatttt aaaataaaaa gagatttata
     9241 ataacaactt tatgttagcg ctaggataag tttttactat atttgattaa ttattaaaga
     9301 agaaggaaaa acacagtttt aaaatactaa acttacaatc aaaaaagaat ctaagctaaa
     9361 ctttgaagat taaagaacag gtacatatag agttccaata taacagaaca gatacgaaat
     9421 taaatttatg ctattgttag attttagatt agaagatgaa gagtatttag aatgcttcat
     9481 gcaaactaat aaaacaaata ttaaaagatg tgcaatagct tcctattaaa tagaaacaaa
     9541 aaagcacttt taaatagtgc tagtttcttg cctgctgaac tcgtttatca tgttcagtga
     9601 tttgtggagt aagggagaaa ctccaaaacg ttcaaacgaa aagtgaaaac tccccatcac
     9661 cctattatca agcattaaga agtaataaag ctacttgctg aatacttaaa aaagcgatac
     9721 aacaaaatgt tgtaatattt tattaaatat gataggctgt aaagccttga aactaagcac
     9781 tttaggcctc tatcctagag tgcttttttg atttctctta gtatccagct ataatcgttg
     9841 agacataact agaccgatat agtccaaagt gatatagtaa aatgaaccaa aaatagtaca
     9901 caatgtggta taatc
//
LOCUS       DBEROU010000061         9333 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.061, whole genome shotgun sequence.
ACCESSION   DBEROU010000061 DBEROU010000000
VERSION     DBEROU010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.061"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            135..821
                     /locus_tag="ACL8CL_001939"
     CDS             135..821
                     /locus_tag="ACL8CL_001939"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HGS1331122.1"
                     /translation="METKKIKNLKGQIIVSCQALEGEPLYTPNGGVMPLLAKAAFQAG
                     AKGIRANSVRDISEIKEEVDLPIIGIIKRDYDGFEPFISATMKEIDELVSEGVDILAL
                     DCTNRSRPGYDNITDFIHDIKVKYPNQLLMADISTFEEGKVAAESGVDFVGTTLSGYT
                     PYSPKKDNPDFELVERLVKELDVPVIAEGRISTPEQARKMLDLGAYAVVVGGAITRPL
                     EIAKKFIEVV"
     gene            822..1754
                     /locus_tag="ACL8CL_001940"
     CDS             822..1754
                     /locus_tag="ACL8CL_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1331123.1"
                     /translation="MNQEILKKSKIQLTVEHIQKYWILYLMMIPATVLLILFTYGPMY
                     GIIMAFQDFTVFKGYTGSPFVGLKHFQRLFSDPLFYRLFKNTFMVGVLDFLFSFPAPL
                     IFALILNEVRKVRFKSVVQSISYLPHFIPLVVMVGIIFELFGSYGIINSLLSSLGMEP
                     ISFFTKSEWFLPLYIGSGVWKTIGWGSIIYMGALTNIDSTLYEAADMDGANRWHKMWH
                     VTLPSLRPTVVTLFILNAGGIMQVGFEKVFLMSSPATYEVSDVLSTYVYRQGILNSDF
                     SYSAAVGLFNNIVALLFVLLANKIAKKLGEEGIV"
     gene            1765..2694
                     /locus_tag="ACL8CL_001941"
     CDS             1765..2694
                     /locus_tag="ACL8CL_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HGS1331124.1"
                     /translation="MIFSKNTKKSNRDKETFVDYLFIFILIIITLLMLYPLWWVVMTS
                     LSEPVWLSKNVVKLFPLKIDITAYKFIFGDSKLWQAYGNSVLYAALSTIVSVAVCSMY
                     AYPLTIPEIRGKKFWNTLILIPMFFSGGMIPLYIQVANLGLLNTAWALVLPGAVSTYY
                     IILFRTQFKSIPMELRESARIDGASEFRIFWNLVLPLSKVIFMIIALYCIVGSWNSFT
                     PPLLYLNDDTKMPLSIYLKNLVVTNTTDTAELSLSAGNTYYQQIAAQGGGIGMRIAIK
                     MGTIVVSVIPIMMIYPFIQKHFVKGILLGSVKG"
     gene            2708..4147
                     /locus_tag="ACL8CL_001942"
     CDS             2708..4147
                     /locus_tag="ACL8CL_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331125.1"
                     /translation="MQNLKKYLLLDSGLVVGYTLENAQLKLNKLKKDTENNPLFGEEY
                     FAENPKLWEVRFDNSYPNVIYDKELKLYRCWYQTFVSDEASEETPLAERGEKEYIVKS
                     SRMTALCYAESKDGVKWEKPNLNLVKFKGSKDNNIVLMHAHGAGIMLDEQELDPKKRY
                     KLVSKLEFSPNLHYMVVVFSEDGIHFDEPIPWKKWNPAADSHNFPFRDPKTGKYAVIT
                     RTWANNVRLSAITFSDDFINWSEPKEILRGDGFEDQIYSMPVISYADIYLGFASMYHE
                     GDTLNENYDKVDLELKFSANLEQWDSVCKGDYLIERGGGVYPQNADFDASCIFTAAPI
                     IEDDKIWFYYMGGNGNHTGFRESSLARGYIKKDQFAYYTNKREGIPAKISTVSFQTYD
                     DEIYILADIPENGYIKVAVGTKYGNAYSDFGQEDISLVQVSDGKYKLEFNKKSILDLG
                     NSPVAFKFEIENAKLYGIEGSIQQHYLKY"
     gene            4216..5793
                     /locus_tag="ACL8CL_001943"
     CDS             4216..5793
                     /locus_tag="ACL8CL_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS1331126.1"
                     /translation="MVKLNKVLKGSVAFLSASLLLAACGNNANSKPAEKTADGKTKIS
                     FLSTWQINDVRPPKDETLVGQWLEEQLGIDIDLVTIPGEGVSSKVNSLITSNQLPTVL
                     LTTGDKSDIAGINKLGKQGAFLDLSQHMDKLPNYKKYLEKNNALAQIEDDEKHIYAFT
                     KFFTDENIMYTTPILRKDLLVGSEFEDLSKIKTVDDYTKVLKYLTEKQGSPAFIQRNG
                     YEGFMKRVTPLWNLSHRTYYDYESDSYKHPVEQPQLKQFVEWLKELRKDNVLHPDWAV
                     MKDETWEGLLASNKGSFTVDRMNIIGDNNFDKPFDWQPLVYPEINGKRYLQPKTPYIS
                     DSGWVINANAPKEEIDKALEFFNFLYADENQEKLAIGFEGKTFTRENPNTQAGIKWLI
                     QIYGENAGNDKAELLFKHGNQAFTRVQTKLDIEAQPGTFPKVMYDQVDKIKKDLGGFR
                     PDTPVLSFTTEELDVLTKSEAGLNTFFDENIIKFIEGQRDMSEWDSFINEAKSKDLDK
                     VTKVYNDAYKRVKDRMK"
     gene            5853..7313
                     /locus_tag="ACL8CL_001944"
     CDS             5853..7313
                     /locus_tag="ACL8CL_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HGS1331127.1"
                     /translation="MYDVVIIGAGSSGALAAIASGRLGLKTCLIEKGSRIGGVPINTL
                     MGSFANLYFDDSGRVNASKIVGELIERIIKKGGTVFNSMEELMNVNDFYQITIPYQPE
                     VYESVLTDMLIESKVDILLNVEVNNIIYDSKNIKSLEILSNQEVKLIDSKVFIDATGN
                     ATIASASGADVYEKNSSYGCLMRVGGVDISKIIEHIEKTKQWNYIEGYNQWLEEVLAN
                     RKYRATGAVLKDPVSYDHAPMLSKDDHFMNDEKWNYIKERWEKFRFIYTLEVSLFRNE
                     LKKAVESGDFNFEIYHDDKSGVTMNGDGISYGSWGQNVALINVAKAFGFNPKYMEDES
                     HANILSRKYNLTFLSFLKKYVPGFENSFILDQGTRATNRSCRHIDNSSMMDEILDFPM
                     YTFKTMYSYQTPKEITYKSIVGGKLNNLFVIGKGAYQSESFRSQISCMLMGISSAAAA
                     KTIIEDKSNTLNINRDLFKSNLDILFTWEFSNWITD"
     gene            7335..7790
                     /locus_tag="ACL8CL_001945"
     CDS             7335..7790
                     /locus_tag="ACL8CL_001945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001000318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HGS1331128.1"
                     /translation="MNDTNKNQYFVYEFDTSFVEMSYSQFFEGYCILFSKEPYPSLSD
                     MPMEFRKEFLMEMSILGDAMLEVLNAYRINYDILGNSTPVLHAHLFPRYMWEEEERRK
                     TVVHRYDSSVFSNPEFEFTIEKHGPIIKKLRSAIDRHYREYQQLLDDSN"
     gene            8295..8744
                     /locus_tag="ACL8CL_001946"
     CDS             8295..8744
                     /locus_tag="ACL8CL_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HGS1331129.1"
                     /translation="MELVEFKNGHVNTVHEHIAITKVKEFLNNHDIPKDKGGIHKIST
                     DFFYNIIDMETTTEENRPWESHKEYYDVHYLLNGEEIILYNFLSQMELSEYKVDDDWQ
                     QMNGTALFSIKLKKDMLLLLEPNDAHKTGLLVEEPINIKKVVFKVKI"
     gene            complement(8789..>9333)
                     /locus_tag="ACL8CL_001947"
     CDS             complement(8789..>9333)
                     /locus_tag="ACL8CL_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HGS1331130.1"
                     /translation="KTSRPFHKVRLMYQDEAGFGRISKLGSCWSPIGVGPHVHSHYIR
                     EFRYCYGAVDAHTGESFFLIAGGCNTEWMNAFLEELSQAYPDDYLLLVMDNAIWHKSS
                     ILKIPTNIGFAFIPPYTPEMNPIEQVWKEIRKCGFKNKAFRTLEDVMNQLQDVIQGLE
                     KEVIKSIVNRRWTRMLFENR"
BASE COUNT         3226 a         1257 c         1735 g         3115 t
ORIGIN      
        1 ctaaaatatt tctataaatt aatttaactt tcctgatagg gctgttcata tcttatttca
       61 attcactata taaataaaat tgtagtagat gttattgaat tagtaaaaaa aataaaaaaa
      121 cggaggtttt ctgaatggaa actaaaaaaa ttaaaaattt aaaaggtcaa ataattgttt
      181 cctgccaagc gttagaagga gagccacttt atactccaaa tggaggagtg atgccgttat
      241 tagcaaaagc cgcctttcaa gcaggcgcta aaggtattcg tgctaattct gtccgcgata
      301 tttcagaaat aaaagaagaa gttgacttac caattatagg tatcataaaa agagattacg
      361 atggctttga accttttatt tcagcaacta tgaaagagat tgatgaatta gttagtgaag
      421 gagtagacat tttagctttg gattgtacta atcgttcaag accaggttat gataatatta
      481 ctgactttat tcatgatatt aaagtcaagt atccaaacca attattaatg gctgatattt
      541 ctacttttga agaagggaaa gtagctgctg aatcgggagt agattttgtg ggtacgacat
      601 tatctggtta tacaccatat agccctaaaa aagataatcc tgattttgag ttagttgaaa
      661 gattggtaaa agagcttgac gtaccagtta ttgctgaagg aagaataagt actccggaac
      721 aagctagaaa aatgttagat ttaggtgctt acgcagtggt tgttggagga gctatcactc
      781 gtcctttgga aatagcgaag aagtttattg aggtagttta gatgaatcaa gaaatattaa
      841 aaaagagtaa gattcaattg acagttgaac atattcaaaa atattggatt ttatatttaa
      901 tgatgattcc agcaactgtt ctactaattt tatttacgta tggaccaatg tacggcatta
      961 ttatggcctt tcaagacttt actgtcttca agggatatac tggaagtcca tttgtcggtt
     1021 taaaacattt tcaaagattg ttctcagatc ctttgtttta tagattgttc aagaatacgt
     1081 tcatggtagg ggttttagac tttttattct ctttcccagc tccgcttatt tttgcactca
     1141 tattaaacga agtcagaaaa gtaagattta agagtgtagt tcaatctatt agttatcttc
     1201 ctcattttat tcccctagtg gttatggttg gtattatttt tgaactattt ggttcatatg
     1261 gtattattaa cagtttactt tcatcacttg gaatggagcc aataagtttc tttacaaaat
     1321 ctgaatggtt cttgcctctc tacattggtt caggagtatg gaaaacaata ggttggggtt
     1381 ctatcattta tatgggagca ttaaccaata tagattcaac tttatatgaa gcagcagata
     1441 tggacggggc taatagatgg cacaaaatgt ggcatgtaac tctaccaagt ttaagaccta
     1501 cagtagttac tttgtttata ctaaatgctg gtggtatcat gcaagtagga tttgaaaagg
     1561 tattcttgat gtctagtcct gcaacatatg aagtatctga tgtattatct acttatgttt
     1621 atagacaagg tattttaaat tctgatttca gttactcagc tgcagtagga ttatttaata
     1681 acatagtagc tttgttattt gttttattag ctaataagat tgctaaaaaa cttggcgaag
     1741 aaggaattgt ataagggggg taaaatgatt ttttcaaaaa atacaaaaaa gagtaataga
     1801 gacaaagaaa ccttcgttga ttatctcttt atattcattt taataataat aaccttactt
     1861 atgttgtatc cattatggtg ggtagtgatg acgtcacttt cagaaccagt ttggctatct
     1921 aaaaacgttg ttaaattatt tcccttaaaa atagatatta ctgcatataa gtttattttt
     1981 ggggattcta aactatggca agcatatggt aatagtgtat tgtatgctgc actatctaca
     2041 attgttagtg ttgcggtatg tagtatgtat gcctatccac ttacaattcc tgaaattagg
     2101 ggtaaaaaat tctggaatac tcttattttg attccaatgt tctttagtgg aggaatgatc
     2161 ccgctttata tacaagttgc aaacctcggt ttattgaata ctgcttgggc tcttgtttta
     2221 cctggtgctg tttctacata ttatataatt ttgtttagaa cacagtttaa atcaatccca
     2281 atggagcttc gtgagtctgc tagaattgat ggggcaagtg aatttagaat tttttggaat
     2341 ttagtattac cattatcaaa agttattttc atgattatag cactttattg catagttggt
     2401 tcatggaata gttttactcc tccattactt tatttgaatg acgacactaa gatgccttta
     2461 tctatttatt tgaaaaattt agttgttaca aatacaactg atactgctga attatcactt
     2521 tctgcaggta atacatatta ccaacaaatt gctgctcaag gtggaggtat tggtatgcgt
     2581 attgctatta agatgggtac tattgttgtt agtgttattc caattatgat gatttatcca
     2641 tttattcaga aacattttgt taagggaatc ctgctaggtt ctgtgaaagg ttaataaggt
     2701 aattattttg caaaatttaa aaaaatatct tttattagat agcggtttag tagttgggta
     2761 tacattagaa aatgctcaat taaaattaaa taagctaaaa aaagatactg aaaacaatcc
     2821 actatttggt gaagagtatt ttgccgaaaa tcctaaatta tgggaagttc gttttgataa
     2881 ttcatatcca aacgttatct atgataaaga attgaaatta tatagatgtt ggtatcaaac
     2941 atttgttagt gatgaagcaa gcgaagagac tcctttagca gaacgtggag agaaggaata
     3001 cattgtcaag tcatctagaa tgacagcact ttgctatgct gaaagtaaag atggagtaaa
     3061 atgggaaaaa ccaaatctca atctagtaaa atttaaaggt tcaaaagata ataatattgt
     3121 tctgatgcat gcacacggtg caggtatcat gctagatgaa caggagttgg atcctaagaa
     3181 acgatataaa ttagtttcaa aattagaatt ttctccgaat cttcattaca tggttgttgt
     3241 tttttcagaa gacggtattc attttgacga acctattcca tggaaaaaat ggaatcctgc
     3301 agcagatagt cataattttc catttagaga tccaaaaact ggtaaatacg ctgttattac
     3361 aagaacctgg gcaaataacg tacgtctaag tgcaattact ttcagtgatg attttattaa
     3421 ttggtcagaa cctaaagaaa tccttagggg agatggattt gaagatcaaa tttattctat
     3481 gccagtaatt tcatacgcgg atatctattt aggttttgct tcaatgtacc acgaaggcga
     3541 cactttaaat gaaaactatg ataaagtaga cttagaattg aaattctctg ctaatttgga
     3601 acaatgggat agtgtatgta agggcgacta tctcattgaa aggggaggtg gagtataccc
     3661 gcagaatgct gattttgatg ctagttgtat tttcacggct gctccaataa ttgaagatga
     3721 taagatttgg ttctactata tgggtggaaa tggtaatcac actggcttcc gagaaagttc
     3781 attagctaga ggatatatta agaaagacca atttgcttat tatactaata agagagaagg
     3841 aattccagct aaaatatcta cagttagctt ccaaacttac gatgatgaaa tttatatctt
     3901 agctgatatt cctgaaaatg gttatattaa agttgcggta ggtactaaat acggtaatgc
     3961 ttattcagat tttggtcagg aagatatctc tcttgttcaa gtttccgatg gtaagtataa
     4021 gttggaattt aataaaaaat ctatcttaga tttaggtaat tctccagttg cgttcaaatt
     4081 tgaaatcgag aatgcaaaat tgtatggtat tgaaggtagt attcagcaac actatctcaa
     4141 atattaacca gaataattcg ttaacaaata atctctatat tagtggagat agatatataa
     4201 aatggaggtc ttattatggt aaagcttaat aaggtattaa aagggagtgt tgcattcctg
     4261 agtgcaagtt tattgctagc agcttgtggc aataacgcta attcaaagcc cgctgaaaaa
     4321 acagctgatg gtaaaacgaa aatttcgttt ctatctacat ggcaaattaa tgatgttcgt
     4381 ccgcctaaag atgaaacact tgttggtcaa tggttagaag aacagctagg tattgatatt
     4441 gatcttgtaa ctattcctgg cgagggagta agttctaaag taaattctct tattactagt
     4501 aaccagttac cgactgtttt gttgacaaca ggagataaga gtgatatcgc tggtatcaac
     4561 aaacttggta aacagggtgc attcttagat ttgtcacaac acatggacaa actaccaaac
     4621 tataagaagt accttgaaaa aaataatgca ttagctcaaa tagaagatga tgaaaaacat
     4681 atttatgctt tcacaaaatt ctttactgat gagaatatca tgtatactac accaattctg
     4741 agaaaagact tacttgttgg ttcagaattt gaagatcttt caaaaattaa aactgttgat
     4801 gactatacaa aagttttgaa atatttgact gaaaaacaag gttctccagc atttattcaa
     4861 cgtaatggct atgaaggctt catgaagcga gttacaccat tgtggaattt aagccatcgt
     4921 acatactatg actatgaatc agattcatat aaacatccag ttgaacaacc tcaattgaaa
     4981 caattcgttg aatggttgaa agaattacgt aaagataacg tacttcaccc tgactgggca
     5041 gttatgaaag atgaaacttg ggaaggtcta ttggcttcta acaagggttc attcactgtt
     5101 gaccgtatga atatcattgg agataataac tttgataaac catttgattg gcagccactt
     5161 gtttatccag aaattaatgg aaaacgttat ttgcaaccga aaacaccgta catttctgat
     5221 tcaggttggg taatcaatgc caatgcacca aaagaagaaa ttgataaagc gttagaattc
     5281 tttaatttct tatacgctga tgaaaatcaa gaaaaacttg ctattggatt tgaaggtaaa
     5341 acatttacac gtgaaaaccc taatactcaa gcaggtatta aatggttgat tcaaatttac
     5401 ggtgaaaatg ctggaaatga taaagcagaa ctgctgttta aacatggtaa ccaagcattt
     5461 actcgcgttc aaactaaatt agatattgag gcacagccag gaacattccc taaagttatg
     5521 tatgatcaag tagataaaat taagaaagat ttaggtggtt ttagaccaga tactccggtt
     5581 ctttccttta caactgaaga attagatgtt ttaactaaat ctgaagcagg attaaatact
     5641 ttctttgatg aaaatattat taaattcatt gaaggacaaa gagatatgag tgagtgggat
     5701 tcatttatca atgaagctaa atctaaagac ttggataaag ttacaaaagt atataatgat
     5761 gcctacaaac gtgtcaaaga tagaatgaaa tagactaaat ggtagctctc tggtttttca
     5821 gagagctact actgcctgag ggggagtaag ctttgtatga tgtagtaatt ataggtgcag
     5881 gaagttcggg cgcattggct gcaattgctt cggggcgtct agggttaaaa acttgtctaa
     5941 tagagaaggg aagtagaatt ggtggagttc ctattaatac actaatgggg tcatttgcta
     6001 atctatattt tgatgattct ggtagagtaa atgcaagtaa aattgttggg gaattaatag
     6061 aacgtattat caaaaaaggt ggtactgtct ttaactctat ggaagaattg atgaatgtaa
     6121 atgattttta tcagattaca attccctatc aaccagaagt ttatgaatcg gttttaactg
     6181 atatgttgat tgagtctaaa gttgatattt tactaaatgt tgaagttaat aatattattt
     6241 atgatagtaa aaatattaaa agtcttgaaa ttttaagtaa tcaggaagtt aaattgattg
     6301 attctaaagt ttttattgat gctacaggga atgcaacaat tgcttctgct agtggagctg
     6361 atgtttatga aaaaaattct tcttacggtt gtttaatgag agttggagga gttgatatct
     6421 caaagattat agaacatatt gaaaaaacaa agcagtggaa ctatatagaa ggatataatc
     6481 agtggttaga agaagtatta gcaaatcgaa agtacagagc cactggagca gttttaaaag
     6541 atccagtttc ttatgatcat gcccctatgc tttcaaaaga cgaccatttt atgaatgatg
     6601 aaaaatggaa ttacattaaa gaaagatggg aaaaatttag atttatttac actttagaag
     6661 tatcactatt taggaatgaa ttaaaaaaag ctgtagaatc tggggatttt aattttgaaa
     6721 tttatcatga tgataaatct ggagtcacta tgaatggcga cggtataagc tatggttcat
     6781 ggggtcaaaa tgtagcatta attaatgttg caaaagcatt tggattcaat cccaaatata
     6841 tggaagatga atcacatgct aatattcttt ctagaaaata taatttaaca tttttatctt
     6901 ttcttaaaaa atatgtacca ggttttgaaa attcttttat ccttgatcag ggaactagag
     6961 ctactaacag atcatgtagg cacattgata attcatctat gatggacgaa attcttgatt
     7021 ttccaatgta tacatttaag actatgtata gctatcaaac tcctaaagaa ataacgtata
     7081 aatcaattgt aggtggaaaa ttgaataatt tgtttgtaat aggtaaaggt gcttaccaat
     7141 ccgaaagttt tagatcacaa attagttgta tgttaatggg gatatcatca gcagcagcgg
     7201 caaaaactat tattgaagat aaaagcaata ctctaaacat taatagagat ttgtttaaat
     7261 cgaacttaga tatattattt acatgggagt tttctaattg gattactgat taacaaataa
     7321 taagggaata ttaaatgaat gatactaata aaaatcaata ctttgtttat gaatttgata
     7381 ctagctttgt agaaatgtct tattctcagt tttttgaagg atattgtatt cttttttcaa
     7441 aagaacccta tccttcttta tctgatatgc caatggaatt tagaaaagaa tttttaatgg
     7501 aaatgagtat attaggtgat gcgatgcttg aagtgttaaa tgcctataga attaattatg
     7561 atatcttagg aaattcaaca cctgtacttc atgctcattt atttccgaga tacatgtggg
     7621 aagaggaaga gagaagaaaa actgtagttc atagatatga ttcttcagtt tttagcaatc
     7681 ctgagtttga atttactata gaaaagcatg gaccaattat taaaaaactg aggtctgcta
     7741 ttgatagaca ttatagagag tatcaacaat tattagatga ttcaaactga actccacatt
     7801 ttcgattaga tttaatacct tgtatattac aactaggttg tggatgatat aatttttcga
     7861 aaaatatatt ttaggaatga cttataaagt aattatatca gaggttatta aatcaatggt
     7921 ccaagcttat gctccttatt cttattttaa ggttggagct gcgttagaaa ctgaagataa
     7981 aaattttttt ctggatgtaa tatagaaaat gttgcttttc tcctacttgc tgtgcagaac
     8041 gagtatattt ttacaaagct gtatttaaag gagaacgttc gttttcaaaa atatgtattg
     8101 taggcggaaa aaaaggtatt ttatcatctt ttatagcatc ttgcagggtg tcttgtcagg
     8161 taatgataga attttgtaat cttgattcat ttgtagttat attggctagt gatgttgata
     8221 cttataaagt atataaactt aaagaactac taccagttag ttttaatcaa gaaaatttat
     8281 aacaggaggg aactatggaa ttagttgaat ttaaaaatgg acatgttaat acggttcatg
     8341 agcatatcgc aataacgaaa gtaaaagagt ttctaaataa tcatgatata ccaaaagata
     8401 aaggtggcat acataaaatt tctactgatt tcttttacaa tataattgat atggaaacta
     8461 caactgaaga aaatagaccg tgggaaagtc ataaggaata ttatgatgtc cattatctgt
     8521 taaatggaga agaaataatt ttatataatt tcttgtctca aatggaattg tctgaatata
     8581 aagtagatga tgattggcaa caaatgaatg ggacagcttt atttagtatt aaattaaaga
     8641 aagatatgtt acttttgttg gaaccaaacg atgcacataa aacaggccta ttggttgaag
     8701 aaccaattaa tattaagaaa gttgttttta aggtaaaaat ttaactgaaa gtactttcag
     8761 ggattaagaa ttttaaaata tttaatactc atctgttttc aaaaagcatt ctagtccatc
     8821 tccgattaac gatggacttt atcacctcct tctccagtcc ttgtatgaca tcttgaagtt
     8881 gattcatgac atcttccaaa gttcgaaagg ctttattctt aaatccacat ttacgaatct
     8941 ctttccacac ttgttcaatg gggttcatct ctggtgtgta tggaggaata aatgcaaaac
     9001 caatattagt cggaatcttt aagatacttg atttatgcca tatagcattg tccataacga
     9061 gtaaaagata atcatctgga taagcttgtg aaagctcttc taaaaaggcg ttcatccact
     9121 cagtattaca tccaccagct attaagaaaa atgattcgcc tgtatgggca tcaacagctc
     9181 cataacaata gcgaaattct cgtatatagt gactatggac atgtggacct actcctattg
     9241 gagaccaaca agatcccagt ttactgattc taccgaaacc agcctcatct tggtacatca
     9301 agcgaacctt atgaaaaggt ctactggttt taa
//
LOCUS       DBEROU010000062         8740 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.062, whole genome shotgun sequence.
ACCESSION   DBEROU010000062 DBEROU010000000
VERSION     DBEROU010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8740
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.062"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..498
                     /locus_tag="ACL8CL_001948"
     CDS             <1..498
                     /locus_tag="ACL8CL_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HGS1331131.1"
                     /translation="IINESVLNVDKKGYELICLQHPVSYDLRRIISVIKISTDIERIG
                     DRIVEILKNLQILQNNEILKKIISEIKILHEVIGLHMNRAISCYREEQSGCLDMVVIQ
                     KQNEIEELSTNIEKKIMNYIFEDDGNVSEVIGALDIIHHLDKIAHTTQSIYKWIMYRK
                     YGNIN"
     gene            511..1986
                     /gene="galT"
                     /locus_tag="ACL8CL_001949"
     CDS             511..1986
                     /gene="galT"
                     /locus_tag="ACL8CL_001949"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0017103 - UTP:galactose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019388 - galactose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HGS1331132.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGTDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYLYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFRFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1999..2978
                     /gene="galE"
                     /locus_tag="ACL8CL_001950"
                     /pseudo
     CDS             1999..2978
                     /gene="galE"
                     /locus_tag="ACL8CL_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996558.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            3188..3847
                     /locus_tag="ACL8CL_001951"
     CDS             3188..3847
                     /locus_tag="ACL8CL_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HGS1331133.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTIVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3872..4939
                     /locus_tag="ACL8CL_001952"
     CDS             3872..4939
                     /locus_tag="ACL8CL_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HGS1331134.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
     gene            5042..6052
                     /locus_tag="ACL8CL_001953"
     CDS             5042..6052
                     /locus_tag="ACL8CL_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1331135.1"
                     /translation="MIKFDNIQIKYGDFVAIDNLNLDIHEGEFFTFLGPSGCGKSTTL
                     RALVGFLDPSSGSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKK
                     VAPDVIKAKVSAVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPL
                     SNLDAKLRVDLRKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPV
                     EIYHNSQTEFVCDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQ
                     DFILKGTIIDVEFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVL
                     QF"
     gene            6064..7755
                     /locus_tag="ACL8CL_001954"
     CDS             6064..7755
                     /locus_tag="ACL8CL_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1331136.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDPNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            7758..8468
                     /locus_tag="ACL8CL_001955"
     CDS             7758..8468
                     /locus_tag="ACL8CL_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HGS1331137.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         2990 a         1193 c         1696 g         2861 t
ORIGIN      
        1 atcataaatg agtcagttct aaatgttgat aaaaaaggat atgagttaat ttgtttacaa
       61 catcccgttt catatgactt aagaagaatt attagtgtaa taaaaatatc aactgacatt
      121 gaacgtattg gggatagaat tgttgaaatt ttaaaaaatc tacagattct tcaaaataat
      181 gaaattttga agaaaattat ttcagaaata aaaatacttc atgaagttat cggactgcat
      241 atgaataggg ctatatcttg ttatagagag gaacaatctg gatgtttaga tatggttgtt
      301 atacaaaaac agaatgagat agaagagctt agtacaaata ttgaaaagaa aataatgaat
      361 tacatatttg aagatgatgg gaatgtttct gaagtaattg gtgctttaga tattattcat
      421 caccttgata aaattgctca tactacgcaa tcaatttata aatggataat gtatagaaaa
      481 tatgggaaca taaattagaa agaaaaatat atgaaaacaa ttattgataa ttttgttgat
      541 agagtaattg aatacggaat gtataatgaa attgacaaaa tatatgtgaa aaatagagta
      601 ctagcgttaa taggtgagga gggaactgat cgcatttccg atgaaaatga cctgaaacaa
      661 ataaaagatt acttagtgga aatcgcctta aaaaacggta aaattaaaga tttaatcgag
      721 gaaaaggaat gtttaggagc agagttaatg aatttcatag ttcctttacc tagtcgatta
      781 aatgatattt tttggagttc atatgatata tcacctcaag aagcagttga agagttttat
      841 aagttaagta aagatagtga ttatattaaa acttctgcta ttgctaagaa tattgagttt
      901 cgagcatcaa ctaaatacgg agagctagag attacgataa atttgtcaaa acccgaaaaa
      961 gatccaaaaa caattgctgc tgaaaaatta gtaaaagcca ctaattatcc caaatgttta
     1021 ctatgtatgg aaaatgaagg atatcagggg cgaataaatt atccagcacg ttcgaatcat
     1081 agaattattc gattgaaact tggtgacgaa gtatggggat tccaatattc tccctactta
     1141 tactttaatg agcatgcgat atttttaaat agtcaacatg taccaatggc tattacctct
     1201 aaaacatttg aacaattatt ggagattgtt gatattttgc caggatattt tgctggttca
     1261 aattctgatc ttcctatctc aggaggctcc attcttagtc ataaccacta ccagggaggg
     1321 aaacatattt tcccaatgga aaaagcaaaa tttgagagtg aatttcgttt taaagacttt
     1381 gaggatgtta atgctggtat tgtaaaatgg ccaatgtcag taattaggtt acaaagtgaa
     1441 aataaaaatc gcttgttaga tttagctact aaaattctaa ataagtggag agaatattca
     1501 gatttagaag tagacatcat tgctatgact gaagatgtcc cacaccatac ggtgactcct
     1561 attgctcgta aagtggacgg gagatatgaa ttagatattg ttctaagaga taatcatact
     1621 actgaacagt acccagatgg tgttttccat cctcatcaag atgtacaaca tattaaaaag
     1681 gaaaatatcg ggttaatcga agtgatggga cttgcgatat taccacctcg tttgaaacca
     1741 gagttagaag aagttggaaa atatctttta ggagaggata acgcaattgc ggattatcat
     1801 ttagaatggg ccgaccaatt gaaagaaaaa tatcctcgca ttaataaaga agaggttaat
     1861 agcgtagttc aacacgaagc aggacaagtg tttgcgagag ttctggaaga tgcaggagtt
     1921 tataagaata caccgtctgg gcatgaagcg tttatgagat ttgttaagtc ggttggaatt
     1981 aattaggagg aaacaaaaat ggcaatattg gtaacaggcg gagctggtta tattggtagc
     2041 cataccgtag tagaattact aaatttagga aaggaagtca tcattgtcga caacctttcg
     2101 aactctagca tcttggtgct agaccgtatt gaagcaatta caggaatacg tcccgtgttt
     2161 tacgaattag atgtttgtga taaaccagca ttgagaaagg tatttgaaca agaatcgatt
     2221 gatgctgcaa ttcattttgc aggttataaa gctgtcggag aatccgtgca aaagcctgtg
     2281 atgtactaca aaataatatt atgagtacat tggcacttgt tgaagtgatg tcagaattta
     2341 atgttaaaaa gattgtcttt tcttcaagtg cgactgtata tggaattaac aatcagtcac
     2401 ctctaattga gactatgcaa acaagtgcga caaatcctta tgggtatacg aaagtgatgc
     2461 ttgagcaaat tttaaaagat gttcatgtgg cagattcaga atggagtatt gcgttgcttc
     2521 gttatttcaa tccaattggt gctcatgagt ctggtttgat tggggaagat ccctcaggaa
     2581 ttcctaacaa cttgatgccc tatattgcac aagtagcggt aggtaagctg tcagaactaa
     2641 gtgtgttcgg aaatgattat gatacgcttg acggtactgg tgtgcgcgat tatatccatg
     2701 tagtagattt agcaatagga catataaaag ctttagaaaa agtatctgaa aaaacagatg
     2761 tttatatcta taacctaggc tcgggagaag gcactagtgt attacaactt gtaaatacat
     2821 ttgaaagtgt taataagatc cctattcctt ataaaatagt tccaagacgt tcaggagacg
     2881 ttgcaacttg ttatgcaaat gcagacaaag catataagga attaaattgg aggacaacaa
     2941 aatcgattga agacatgtgt agagatacat ggaattagca atcaaaaaat cccaatggct
     3001 ataattgatg atactgtcta tatgagtgct gtattttgga aaaattactt aaccttttaa
     3061 tgaaatgcat atttaatgaa agataaatta aaaaaaatgt ttgacagcgc ttgacaaagg
     3121 aaacggtttc attgtataat gtaaacatag tgacaccgat gtcaaatata aaaaagaagg
     3181 tctttatatg aaaaacttaa caaaaattaa gttcaaagag aatggggaat ttaatcattt
     3241 ccctgggaat acaattgtag caaatcttta tactaaacaa gatttgatgg aagttgttga
     3301 tattattcaa tcacgttata gagaattacc atttatcgat aagtttactt taactccaag
     3361 gaattctatt cacatgactg taattgaatt gttgtgccat gaaaatcgtg aaacggagtt
     3421 ttggagcagt aatcttcctc tagatacacc tttacaggaa atacatgatt actttgctaa
     3481 acaacttgaa atttttccat tgttggatga agaaattcat atgcgtataa ctgaaatggg
     3541 aaaacaaaac atactagttg aacctgcaga tgaagcttct gcaaagagat tagaagaaat
     3601 tcgtacttat gtctcagaaa aagcaggtgt ttgtttccct aatcatgata gatatcaatt
     3661 ccatatttca attgggtatc ttcggattcc tctaaccgaa gaggaggaag aagagtttac
     3721 taaagtcaga gcagaattaa ctgaaatttt attagagaag attccaacta ttactgtaaa
     3781 ccgtattgac tatactgtat ttgaagatat gagacaattt gttccatatc acgaaaaatt
     3841 taaataaaat aataaaaaag gagaacttat catgaaaaaa atgaaagttt ggtctactgt
     3901 acttgcaacg ggagttgctc ttactacact tgctgcttgc tctggaggtt caaattctac
     3961 gactgcttct tcatctgaag aaaaagctga taaaagtcaa gaattagtaa tctattcgaa
     4021 ctcagtctca aatggtcgtg gtgattggtt aactgctaaa gcaaaagaag ctggttttaa
     4081 tataaaaatg gttgatattg ctggcgctca attagcagac cgtgttattg ctgagaagaa
     4141 taatgcagtt gcagatatgg tatttggaat tggtgctgtt gactcaaata aaattagaga
     4201 tcaaaaatta ctagtacagt acaagcctaa atggttagat aaaattgatc aatctttatc
     4261 agataaagat aattattata atcctgtgat tgttcaacca ttagttttaa ttggggcgcc
     4321 tgatgtaaaa gaaatgccta aagattggac tgaattaggt agtaagtata aaggtaaata
     4381 ttcaatttat ggtcttcaag gtggtacagg acgggcaatt ctagcaagta tcttagttcg
     4441 ttaccttgat gaaaaaggag atttaggcgt atcagaaaaa ggatgggaag ttgcaaaaga
     4501 atactttgca aatgcctata ctcttcaaaa aggtgaaagc tcagttgtta aagcgttaga
     4561 taaagaaagc ccaattcaat acggtatgat ctggggttct ggagcgttaa tcggacaaaa
     4621 agaacaaaat gttgaattca aagttatgac tcctgagatt ggtgtaccat ttgtaactga
     4681 acaaactatg gttttaagca ctagtaaaaa acaagcgtta gctaaagaat ttattgattg
     4741 gtttggtcaa tcagaaattc aagtagaata tagtaaaaaa tttggatcta ttcctgcaaa
     4801 taaagatgcc ctaaaagagt tacctgaaga tacgaaaaaa tttgttgatc aagtgaaacc
     4861 acaaaatatt gactgggaag ctgttggaaa gcatttggat gaatgggtag aaaaagctga
     4921 attagaatac gtaaaataag aaataagagt tattttttat gtccaagtct ctctattatt
     4981 tctaaattta aggaagaaat aaagagaggc ttttggttta gtaatagagg gagttagtga
     5041 gatgattaaa tttgataata ttcaaattaa atatggtgat tttgttgcaa ttgataatct
     5101 gaatttagat atacatgaag gggaattctt tacatttctt gggccttcag gatgtggtaa
     5161 atcaactact ttgagagcat tggtaggttt tttagatcca tcatcaggaa gtattgaagt
     5221 taatggaaca gatgtcactc atttggaacc tgaaaagcgt ggaattggta ttgtatttca
     5281 atcttatgcg ctatttccaa ctatgactgt ttttgataat attgcatttg gtttaaaagt
     5341 taagaaggta gctccagatg ttattaaagc taaagtatca gcagtggcag caaaaattaa
     5401 gatctctgat caacagttac agcgtaatgt atcagaatta tctgggggtc aacaacaaag
     5461 ggtagcattg gctcgtgctc tggttcttga acctaaaatt ctttgtctag atgaaccatt
     5521 gtcaaacctt gacgcaaaat tacgtgtaga tttgagaaaa gagttgaaaa gacttcaaaa
     5581 agagttaggt attactactt tatatgttac tcatgatcaa gaggaagcct tgactttatc
     5641 tgatagaatt gcagtcttta acaatggata catcgaacag gtcggtacac cagtagagat
     5701 ttatcataat tctcaaactg aatttgtatg tgattttatt ggagatatta atgttttgac
     5761 cgatgaaaca gtccacgaag tattactgaa aaatacaagc gttttcttag aagataaaaa
     5821 aggatacatt cgattagaga aagttcgatt caatcgtgaa actgaacaag atttcattct
     5881 aaaagggaca attattgatg ttgagttttc tggagttaca attcactata caataaaagt
     5941 ttctgaaagt cagattctta atgtaacaag tattgatagt caggctgcta ttagatctgt
     6001 cggagaaagt gtggaattat ttatcacacc atcagacgtt ctgcaatttt aaggaggtgc
     6061 agtatgcgtc ataaattaaa tttaaaagat tggcttattc gtttagggtt aatctggttc
     6121 ttagtaacat ttattattta tccaaacttt gatctagtag tgaatgtatt tgtaaaagga
     6181 ggagaatttt cccttgatgc tgtacatcgt gttctaaaat ctcagagggc acttcagagt
     6241 attatgaaca gttttaagtt agcattttca ctcattatta cagttaatgt cgtaggtatt
     6301 ctttgtgttc tatttacaga gtactttgat attaaaggtg ctaaaatttt aaaattaggt
     6361 tatatgacct ctttaattta tggaggagtg gttttagcga ctggatataa atttgtctat
     6421 ggtccatatg gattgattac aaaattttta caaaatgtta tcccttcttt agaccctaac
     6481 tggtttattg ggtatggtgc agtcttattc attatgacat tttcaggaac tgctaatcat
     6541 acattgtttt taacaaatac aattcgaagc gttgactatc acactattga ggctgctcga
     6601 aatatgggag caaaaccatt tactgttttc cgaaaagtag tgttaccaac cttaattcca
     6661 actctatttg cacttactat tatggttttt cttagtggtt tatctgcagt agcagcaccc
     6721 atgattgttg gtggtaaaga atttcaaact ataaatccaa tgattattac atttgcaggg
     6781 atggggaatt ctcgtgattt agctgcctta cttgcaatta ttttaggtat tgcaactaca
     6841 attttgctta ctatcatgaa taagatagaa aaaggtggaa attatatttc tatctctaag
     6901 actaaagcgc ctcttaaaaa acaaaaaatt gcgtctaagc cttggaatat cattgctcac
     6961 attgtagcat atggattgtt cacagttttc atgcttccac taatttttat agtattatac
     7021 tcatttacag atccagttgc aattcaaaca ggtaacttaa cattatcaaa ctttacttta
     7081 gaaaattatc gcttattctt tagtaatagt gcggcattct ctccattctt ggtcagcttt
     7141 atttattcta ttattgctgc gacaacagca acaattctcg cagttgtatt tgctcgtgtt
     7201 gtcagaaaac ataaatctcg ttttgatttc ttatttgaat atggtgctct acttccttgg
     7261 ttattaccaa gtacgctttt agcagtaagt ttattattta cttttaatca gccacaattt
     7321 cttgtcttga atcagatttt ggtaggtagt ttggtaattc tacttattgc atatatagtt
     7381 gtaaaaatcc cattttctta tagaatggta cgtgctattt tatttagtgt tgatgatgag
     7441 atggaagatg cagcaagaag tatgggtgct tcaccttttt atactatgat gaaggttatc
     7501 attccattta ttttaccggt tgttctctct gttattgctt taaactttaa ctctttatta
     7561 actgacttcg acttatctgt attcctttac catcccctag ctcaaccatt aggtattacg
     7621 attcgatctg caggtgatga aacagcaaca tctaatgcac aagctctggt atttgtttat
     7681 acaattgttc tgatgattat ttctggaacg gtattatact tcacacaaag accggggcgt
     7741 aaagtaagga aataatcatg acagccacta gtcttgggtt atcaaatatt gaaatagttg
     7801 tcaggattgt tttatcagta gtcattggta gtataattgg tttagagaga gggagcaaat
     7861 cccagcctgc aggcatccga acttatagta ttgtttgtct agctgcatgt ttgattatga
     7921 tgacgaatga atacgtatct tataaatttg ggacaggaga tcctacacga ttaggagctc
     7981 aagttatatc aggtgtgggt tttctaggcg ctggaacgat tcttattaca gataaaaaga
     8041 aaattacagg tctgacaact gcagcaggca tttgggcttc ggcaggaatt ggattagcta
     8101 ttggagtagg tttttatgag ggagctcttt tagtagccat ttctgtttgg ggtgtgatat
     8161 ccatgttcca accactaaaa aaatatctac aaaatcgttc taaaatgatt gaattgtata
     8221 tagtggttaa atctacagaa gcctataatc gtgtattggt ttattgtgca gaaaatggta
     8281 tcagaatgac cgattcaaga acagcgtttg gagatgttaa ttctgacaga attgaatact
     8341 ttgatgttcc agataaaaaa atagcgtcat ttattactct ggaattatca ggtagatttg
     8401 aacatcttcg actgatggaa gaaattgcta atattgtagg tgttatttat gtcgaagaaa
     8461 ttagctgaaa tttagtaaac aaagggacag atgtttttgt ccttttgttt tttagatgtt
     8521 ttaatcatct aatttttgag tagatttttg ctcagaggtt tttatgaaag attataaaat
     8581 caattttgat ttaggtaaaa tagaatattt tgataataat tgtttgatac aagtatacaa
     8641 atttatttct ttttatgata tttgtgaaat ggtgtttgcc tttcatttac ctcctgatga
     8701 gttgataaca aatgttatct ttaaagaaaa aattaactct
//
LOCUS       DBEROU010000063         8506 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.063, whole genome shotgun sequence.
ACCESSION   DBEROU010000063 DBEROU010000000
VERSION     DBEROU010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8506
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.063"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(123..1319)
                     /gene="tuf"
                     /locus_tag="ACL8CL_001956"
     CDS             complement(123..1319)
                     /gene="tuf"
                     /locus_tag="ACL8CL_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HGS1331138.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2230..3099)
                     /gene="gla"
                     /locus_tag="ACL8CL_001957"
     CDS             complement(2230..3099)
                     /gene="gla"
                     /locus_tag="ACL8CL_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaglyceroporin Gla"
                     /protein_id="HGS1331139.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3323..3844)
                     /locus_tag="ACL8CL_001958"
                     /pseudo
     CDS             complement(3323..3844)
                     /locus_tag="ACL8CL_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(3802..4908)
                     /locus_tag="ACL8CL_001959"
     CDS             complement(3802..4908)
                     /locus_tag="ACL8CL_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HGS1331140.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEVDTNSASLTGNIVSEGRTVTLVYRESEAPTTVTVTANY
                     YKEGSQEKLAESVIKADLAIGSEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5019..5273)
                     /locus_tag="ACL8CL_001960"
     CDS             complement(5019..5273)
                     /locus_tag="ACL8CL_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331141.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRTTNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRAYKLLKTLIPGENYLSC"
     gene            complement(5267..5509)
                     /locus_tag="ACL8CL_001961"
     CDS             complement(5267..5509)
                     /locus_tag="ACL8CL_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000284543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331142.1"
                     /translation="MVQNEDLYKKGLNVELAHQQIKGFFEAEFKNRINGVLNTKIKNS
                     TLNRVNKKTIHQSNKNSMINLKQKQRKMLKNKAILC"
     gene            complement(5613..6457)
                     /locus_tag="ACL8CL_001962"
                     /pseudo
     CDS             complement(5613..6457)
                     /locus_tag="ACL8CL_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078372828.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            6691..8409
                     /locus_tag="ACL8CL_001963"
     CDS             6691..8409
                     /locus_tag="ACL8CL_001963"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HGS1331143.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQMPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDIEYLKEVKDINI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
BASE COUNT         2419 a         1750 c         1609 g         2728 t
ORIGIN      
        1 tattatagtt tgctctttga ttttcattga gtattagttc aatatctaaa aaaagaacct
       61 tgccaagcaa gattctttta gtgtctgact taaataattg ttcttctggg aactaaatcg
      121 aattaagctt cgatttctgt aaccatacct gaaccaacag tacgtccacc ctcacggata
      181 gagaatgtag taccttgttc tacggcgatt gggtgaatca actcaacgtc gattgtcacg
      241 ttatcaccag gcattaccat ttcagtacct gctggaagtt cgattgaacc tgtaacgtca
      301 gtagtacgga agtagaattg tggacggtag ttgttgaaga atggagtgtg acgtccacct
      361 tcttctttag taaggatgta gacttcacct ttgaatttag tgtgtgggtt gattgaacct
      421 ggtttagcga taacttgtcc acgttcgatt tcatcacgtt gaacaccacg aagaaggaca
      481 cctacgttat ctccagcaag accttcgtca agttgtttac ggaacatttc aacaccagta
      541 acaactgctt tttgagtttc ttctttgata ccaacgattt cgatttcgtc gttgacttta
      601 acgataccac ggtcgatacg tcctgaagca actgtaccac gtccagtgat tgagaatacg
      661 tcctcgactg gaagaagcaa tggtttgtca gtgtcacgtt ctggttctgg gatatactca
      721 tcaactgtgt tcatcaattc cataacgatg tcttcgtatt tagagtcacc ttcaagagct
      781 ttaagtgctg aaccttggat aactggaaga tcgtcacctg ggaagtcgta ttctgacaat
      841 aggtcacgga tttccatttc aaccaattca agcaattctt cgtcgtcaac caagtcaact
      901 ttgttcatga agacgataag gtgtttaaca ccaacctgac gtgaaagaag gatgtgctca
      961 cgagtttgtg gcattggtcc gtcagttgaa gctactacaa ggatagctcc gtccatttga
     1021 gcagcaccag tgatcatgtt tttaacgtag tccgcgtgtc ctggagcgtc gatgtgagcg
     1081 tagtgacgtt tttcagtttc gtactcaacg tgcgcagtgt tgatagtgat accgcgttcg
     1141 cgttcttctg gagcagcatc gatagacgca tagtctttag gttggttaac tgatgaaggc
     1201 aagcgacgtg ccaaaacagt tgtgatagct gcagttaggg tagttttacc gtggtcaacg
     1261 tgtccgatag taccaatgtt aacgtgtggt ttactacgat cgtatttttc ttttgccatt
     1321 tgagtaaaag cctccaataa aatatatttt atagatagac agtaggcaat acagtctaac
     1381 tttccttact attttatcaa atttcagcta aattgcaagt gttttacaac gtttttgtga
     1441 attattcttg gtaaaaattt aactaattaa tagaaagtac taaatagact tatccttcaa
     1501 ggagttttca gcattatcca tccactcact tgctgaaaat gtaaacagtc tctggtttat
     1561 atagtaaaat gaaacaagaa tagggcaaat cgatcaggac agtcaaatcg atttctaaca
     1621 atgttttaga agtagatgtg tactattcta gtttcaatct actatacaac ctcaaaactg
     1681 tgttttgaac aacctgcggc tagcttctta gtttgccctt tgattttcat tgagtataaa
     1741 attgcagata aaacgataac aatcatagga ctcagcagag tcaatgtagg tcgaagaata
     1801 gcccccctaa tcgcttattt tatagcaaaa tgaaatagaa actgaacatt ttgttgagga
     1861 aggtaaatca attcctcaca atgtttgaga aagaagggag gactattcca acttcaaccc
     1921 actataaagc agacttacaa gatagggaac ccatctccga taatatatag tagaatgaaa
     1981 actgcacaat ttgattggag aattcaaacc aatttctaac aatgttttag aaacaagggt
     2041 ctattatttc aactgcaacc tactatagcc cattgaagct ggaatagccc actgagactt
     2101 ctaaaccact gctggagagg agtttgttta gagtttcttt catttcccta aaaaatcaaa
     2161 aagagagaac cagtctgatc ctctcttact tatagatggt gctcactcaa atgttaaagg
     2221 agctatttct tagagataaa ggaatttgaa tacagctact gccgcaattg ctgctgcgat
     2281 aggtgctact actggtaccc aagaatacca ccattttgaa tcgcctttat gctcaccaag
     2341 aactgatttt ggaaggaaag catgaaggag acgtggtccc aagtcacggg ctgggttcaa
     2401 ggcaggtcct gtaggtcctc caagtgatgt taccaaagcc ataacgagga atccaagtgc
     2461 caagtgagcc acagaaagtc ctgaagcagt gtgtggagcc acctgtgctt taatagccaa
     2521 gtcagaaaaa tcaactgttt gtcctgcttc tattgccttt tgtttcatga attgaagcac
     2581 ttcagcacca aagaagtttt tagtcaaacc aagagctgca aagaacaaaa caaatgaacc
     2641 aacaaactca ttaatcaaac cattgacagt tgctgcatag cgactttctt ttgtaccatg
     2701 gtcaatactt gaaatagttg agaaagttcc caagatgtta tttgggtttt cagttttcaa
     2761 gtagaatgga cggtatgttg ccacaactaa ggcttggcca aagatagccc ccaagacttg
     2821 cgcgataatg taaggtacca cttgtgccca agggaaaaga ccgctaactg caagccctag
     2881 agtgaaagca gggttgatgt gattcccaga tacgttacca aacatcaagg ctgggatcat
     2941 aacccccata ccataaccaa cagcgatgac gatccagcca ctttggtgac ctttcgtacc
     3001 tttaagttca acgttggcaa ctgcaccatt cccaagaatg atcaaaatgg cagttcccaa
     3061 aaattcagtg gcatacttca gtgcccatgt gaaatccatt tgctagattc tccttaaaat
     3121 tttttagata ttccttattt tatcaatttt taccctattt agcaactgat ttctggaaat
     3181 atttttaaaa aaataggatt catagtttcc cagttcaacg gacttgtaag cgtttttatt
     3241 cttcacgaaa gacctcgttc tcaaaaccag gaaaaaagcc cacatctgtg agcttttcct
     3301 tgttcctaga ttctaaaatc tattattttt catcttcttt tttcttgaag aatcctaatc
     3361 ctagaccagc taacaaggtc atgataccag cactagccaa ggtagcatta gcttctgtac
     3421 ctgtgtttgg caattcctct ctcttacctg tctcataagt cggaacttct gggtctggat
     3481 tcactggagt ttcagttttt ggagtacctg gttctggagt tggtttatct ggtgttgata
     3541 aacggtcata ccttaccgtt atttctttat cactagagtc tgacgtaact tcttgtgatt
     3601 aaactgttgg aatatctgga gctttgtact tgtcaatctt accagatata acctcgtccc
     3661 agtttcctgt tgtccattca ccgtaggtta caactcccgt gaccttgttc tcagtttttg
     3721 tacggcttaa ggttacaggt tgaacaacat cttcttttac attttggttc gtaactttat
     3781 caacgtaatg aatgatacgc gttatagtct tcgtctcagt agaggttgct gttttgggaa
     3841 ccactgtttc ctcaacattc tcacggtagt aatagtcaac tgttgcaccg tcttctggta
     3901 cgcatttgca ggagttgcta ccaaggtgta tgttgttttc cttgtgataa ctcggtcttc
     3961 tttgtcctca gttgttgttt tcccttcaat agtttttgat tctgtggtat actcagaacc
     4021 tatcgctaaa tcagctttta taacagactc tgccaacttc tcttggctac cttctttata
     4081 gtaattggct gttactgtaa cagtggttgg cgcttcgctt tctctataaa ctaaggtcac
     4141 tgttctacct tcgcttacaa tattcccagt taaacttgca gaatttgtat ctacttcttt
     4201 aaaagtataa tattttccgt cagtagtagt catgctactg agttttttat ctgtgacatt
     4261 gtagctggta ccaatcagtt gttttttatt ggtaatgtag gttccgtcac tttctttttc
     4321 tccaattcca gtatcatttt tcaatgatag caaacgccct tgttcatcaa catagcgaac
     4381 tttcacattt tctgagatta gttctgccaa ttctgaagtt tttttctttt tcttgatttc
     4441 ttcggttatt ttccctttct cttcttcggg aatatttagt tttggaatga ttttttcaac
     4501 aacggttcgt gatggttcca cagtatcttg gatgactgaa aagtcagcta gaattgggag
     4561 attatactga acacggtgac tttgagtgtt tactcctact ctttcattat tctctgaaaa
     4621 tacttgtacg gtataagaaa caacatcttt tcctaataga acatccccag tagagaaata
     4681 gccgcctttt cctagtttgc tatctccaga gcccacttct ttcctaatct tatcagatag
     4741 ttttttacca gtcagtacat tcgttcgcac aatccctttg tctaccccta caaagtggga
     4801 gaactttttg aactcttcag aaccagatct agcccaacca ttattaaggg catttgcttt
     4861 tgtatttgta ttctctctca aaagtttggc gattagaatt atattcatcg gcacttagag
     4921 ttgctgctat atctgactct tgaataccaa cttccttact accatttcta gcggcagtat
     4981 atgtgtattt atctgtttct acttctagct gattatcctt aacatgatag gtaattttct
     5041 cctggtataa gagtcttcag tagtttatat gctctgtcat catctttagc tttagaagca
     5101 gctgagttta gccaagttgg ggtattagga gtatctttat cagcctgttt caagacatta
     5161 ttcaagtcat ttgctagcct attggttgtc ctcactgtga aagttttctt ccagtctgaa
     5221 ccacttttat tagacttaga tatgctcatg aaatcctggt caacattcaa cacaatatcg
     5281 ccttgttttt tagcatcttc cgttgcttct gcttcaaatt gatcatggag tttttgttgc
     5341 tctggtgtat agttttttta tttacacgat ttaatgtact attttttatt ttagtattaa
     5401 gaactccatt aatacgattt ttaaactctg cttcaaaaaa tcccttaatt tgttggtgcg
     5461 caagttcaac attcaaacct tttttataca agtcttcatt ctgaacgatt tcggctacac
     5521 ggtgaatggt ttgattgtat accgtcttct cttaaccagc agtatcctta gttttaatga
     5581 ctactcgata ggttccttta gaaccgttat agtcatctag ttttaaaaaa gcattccaaa
     5641 cattcaaaaa tagttggaac ttcccttaat aaatccgtca ctttcttttt caagatagcc
     5701 caagcttgct caataggatt caaatccggt gaataaggtg gtagaggtaa gaaaaagtgt
     5761 ttatcttgga tgcagagttc atccaaaatg ttcttgggat gaaaaccagc attgcccatg
     5821 acaataacat gaggtgtctt caaagcaggt aggagttgcg ttttgaacca ctccacaaag
     5881 aaatcgcttg tcatgctttt cttgtaaatc atgggagcta taaactctcc gtctacttgt
     5941 cctgcaacaa ttgaagttcg ctcaaaacga cgtccgctaa tcttttcata gactttctcc
     6001 cctctaggag cccctgcata aggacgatag agatagcggt cgattcctgt ttcatcaata
     6061 taaacaggtg ctaggtgctt taaactatta aaattcttaa gaaataaggc tacttttctg
     6121 ggtcttgttc cttaaagctc gtcatctttt tttaaagtga ccttaatctg ctttaaagct
     6181 gcccatactg aaggaacagc acaatcaaaa tgtgccgcaa tttcccgtaa aaaagcatct
     6241 ggatgagcct ctacaaaggc tttcaattcc tctaaaggaa tctttcggtt tttgacgact
     6301 cgctttttcc tctctatgtg ttcttgttca cgtctttctt ttcccacgtg aagagagttc
     6361 tgacgccaac accaaaaaac ttggctgcct cgacatggct gtgcccctct ttgatgtaat
     6421 ctaatgctcg ctgtttaaaa tcagtactat atgccatagc tttattataa tatgcttgtt
     6481 taaaactaag taactatatc aatgttttgt tatgagaaga cttacttttt ttactgaaat
     6541 tgaatttttc ctagcctcaa catcatataa ccccaaaatt caatctgacc tggtttgaag
     6601 agattttcga agcagataag gttttactaa aactaaaaaa aatgatataa tcaaactgat
     6661 taaatgtttt aaaaataaag gagaatttga atgtcttacc aagaaaatta ccagaaatgg
     6721 gttgattttg tggagcttcc tgactacctt cgtcaagatt tggaaaatat ggacgaaaaa
     6781 actaaggaag atgccttcta tacaaatctt gaatttggta ctgcaggtat gcgtggcttg
     6841 gttggtgctg gtacaaaccg tatcaacatc tacgttgttc gccaagctac tgaaggattg
     6901 gctcgtttga ttgagtcaaa aggtggaaac gagaaagaac gcggtgtagc aattgcctac
     6961 gatagccgtc acttctcacc tgagtttgcc tttgaatctg cggcagttct tgctaaacac
     7021 ggcatcaaat cttacgtatt tgaaagcctt cgtccaactc cagaactatc atttgcagtt
     7081 cgtcatctca actgtttcgc aggtatcatg gtcacagcca gccacaaccc tgctccattt
     7141 aacggttaca aggtttacgg tgaagacggt ggacaaatgc ctccacacga tgcggacgct
     7201 ttgactactt atatccgtgc aatcgaaaac ccatttgcag ttgaagttgc tgatgtggaa
     7261 actgaaaaag cttctggctt gattgaagtt atcggcgaag ctgttgacat agaatacctt
     7321 aaagaggtta aggacataaa catcaaccca gccttgattg aagaatttgg taaagacatg
     7381 aagattgtct acacaccact tcatggtact ggtgaaatgt tggctcgtcg tgctcttgcc
     7441 caagcaggat ttgactctgt tcaagttgtt gaagcgcaag caactgctga cccagacttc
     7501 tcaactgtaa catctccaaa cccagaaagc caagcagcct ttgcccttgc tgaagaactt
     7561 ggtcgtcaag ttggtgcaga tgttcttgtc gcaactgacc cagacgctga ccgtgttggt
     7621 gttgaagttc ttcaaaaaga tggtagctac ctcaaccttt caggtaacca aatcggtgct
     7681 atcatggcta aatacatctt ggaagctcac aaaaacgctg gaactcttcc tgaaaatgcc
     7741 gctctctgca aatctatcgt ttcaactgac ttggtaacga agattgctga aagctacggc
     7801 gcaactatgt tcaacgtctt gacaggtttc aaatttatcg ctgaaaaaat ccaagaattc
     7861 gaagaaaaac acaaccacac ttacatgatg ggatttgaag aaagcttcgg ttacttgatt
     7921 aaaccatttg tacgtgataa agatgccatc caagccgttc ttgtcgttgc tgaacttgct
     7981 gcctactacc gttctcgtgg tttgacactt gctgacggta tcgaagaaat ctataaagaa
     8041 tatggctact acgcagaaaa aacaatctct gttactcttt caggtgtcga tggtgctgaa
     8101 caaatcaaag cgattatggc taaattccgc aacaatgctc caacagaatg gaacgcaaca
     8161 gctatcactg tcgtagaaga cttcaaggca caaactgcta ctgttgctga cggtactgtt
     8221 acaaacttga caactcctcc aagtgatgtg ttgaaataca cacttgctga cggttcatgg
     8281 attgccgttc gcccttcagg tacagaacca aaaatcaagt tctacattgc agttgtaggg
     8341 gaaaccaacg aagaatcaca agctaagatt gctaacatcg aagcagaaat caatgcattt
     8401 gtaaaataaa gacctataaa agatggcttt ttgtcaactg tagtgggttg aaaaaaagct
     8461 aagcttgaga aaggacaaat ttcgtccttt cttttttgat attcag
//
LOCUS       DBEROU010000064         8006 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.064, whole genome shotgun sequence.
ACCESSION   DBEROU010000064 DBEROU010000000
VERSION     DBEROU010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 8006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8006
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.064"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..555
                     /locus_tag="ACL8CL_001964"
     CDS             <1..555
                     /locus_tag="ACL8CL_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HGS1331144.1"
                     /translation="KKQASTNFKPAGQSIEQRSEAINLRLENGHYEIDTVLLTRAKNY
                     CLLVLTDRKSRHQIIRLIPNKSAEVVNQALKLILKQHKILSITADNGSEFNRLSNVFS
                     EEHIYYAHSYASWERGTNENHNRLIRRWLPKGTKKMTPKEVAFIEKWINNYPKKYLDY
                     KSPREDFWMANLNLKFSVRNKSRN"
     gene            551..1225
                     /locus_tag="ACL8CL_001965"
     CDS             551..1225
                     /locus_tag="ACL8CL_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HGS1331145.1"
                     /translation="MDSNFAGRLNILRAGVLDANDGIISIAGVVIGVASATTNIWIIF
                     LSGFAAILAGAFSMAGGEYVSVSTPKDTEEAAVSREKLLLDQDRELAKKSLYAAYIQN
                     GECKTSAQLLTNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPML
                     SVTIFPSEYRIPATVLIVGVALLLTGYTSARLGKDPTRTAMIRNLAIGLLTMGVTFLL
                     EQLFNI"
     gene            complement(1267..3255)
                     /locus_tag="ACL8CL_001966"
     CDS             complement(1267..3255)
                     /locus_tag="ACL8CL_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1331146.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTITYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVLFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMKVVITVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKKGRFLPLQTILGSISLGIGYYLAL
                     TVKDPLTALTTFFIAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYKIKEKEVLRYTYFAVANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFVKNDGLKGQKTLTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSAIYNQFYGGMNVDASEEEQLKVAEEYENYLNQF
                     NAQLDTEGSYVYGSNLADASSQMSALFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(3257..4015)
                     /locus_tag="ACL8CL_001967"
     CDS             complement(3257..4015)
                     /locus_tag="ACL8CL_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HGS1331147.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFNEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVMASEVN"
     gene            4443..4634
                     /locus_tag="ACL8CL_001968"
     CDS             4443..4634
                     /locus_tag="ACL8CL_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331148.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFISTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            complement(4988..5260)
                     /locus_tag="ACL8CL_001969"
     CDS             complement(4988..5260)
                     /locus_tag="ACL8CL_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HGS1331149.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(5318..5767)
                     /locus_tag="ACL8CL_001970"
     CDS             complement(5318..5767)
                     /locus_tag="ACL8CL_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HGS1331150.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            complement(5754..7883)
                     /locus_tag="ACL8CL_001971"
     CDS             complement(5754..7883)
                     /locus_tag="ACL8CL_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HGS1331151.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRAMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
BASE COUNT         2397 a         1682 c         1566 g         2361 t
ORIGIN      
        1 aagaaacagg ctagcaccaa ctttaaacct gctggtcaat ccatcgaaca gcggtctgaa
       61 gctatcaatc ttcgcttgga gaatgggcat tatgagattg atacggttct acttacgaga
      121 gcgaaaaact actgcttgct tgtcttgacg gatcgaaaga gtagacatca gatcatccga
      181 ttgattccaa ataaaagtgc tgaggtggtc aatcaggctc taaaactcat cttaaaacaa
      241 cacaagattc tttccatcac ggcagataat ggatcggaat tcaatcgctt gtctaatgta
      301 ttttctgagg agcacattta ttatgcacat tcctatgcct cttgggaaag gggaactaat
      361 gagaatcaca acaggctcat tcgtagatgg ttacctaagg gaaccaagaa aatgactccc
      421 aaagaagtcg cattcatcga aaagtggatc aacaactatc ctaaaaaata cttggactac
      481 aagtcaccca gagaagactt ctggatggct aacttgaact tgaaatttag cgtcagaaac
      541 aaatcacgaa attgattcaa attttgcagg tcgtttaaat atcctgcgtg cgggtgttct
      601 tgatgctaac gatggaatta tttccattgc tggtgtggtt atcggagttg ccagtgccac
      661 gaccaatatc tggattatct ttttatcagg ttttgcggct atcttagctg gtgccttttc
      721 aatggctggt ggagaatatg tatccgtttc aactccaaaa gataccgagg aagctgccgt
      781 ttcgcgagaa aaactcttgt tagaccaaga tagggaacta gccaaaaaat ccctctatgc
      841 tgcttatatc caaaatggag aatgcaaaac ttctgcccaa ctcttgacca ataagatctt
      901 tcttaaaaat ccactcaagg ctctggtaga ggaaaaatat gggattgagt atgaagaatt
      961 taccaatcct tggcacgctg ccatttctag cttcgttgcc tttttcctta gaagtttgcc
     1021 tccaatgctg tcagtgacca tattcccaag tgaatatcgc atccctgcta ctgtccttat
     1081 tgtcggtgtg gcccttcttc tcactggtta cactagtgcc agacttggaa aggatccgac
     1141 tagaacagct atgattcgga accttgctat tggtctcttg accatgggag ttaccttcct
     1201 gctcgaacaa cttttcaaca tttagaatac aagaaatacc tcgattttga agtcgaggta
     1261 tcttttttac atttgcacaa tcttgcgata acttcttgaa gtaatcatga aaatcagcac
     1321 ataggcgatg aggaagatag cgcagataga caaggtcaca atcaacatca tagtcgtatc
     1381 cagtacacca atcactttta aaatcaggct aagcatatgg taggcaaagg cgagatgtat
     1441 gaaggcaaag agcaaaggaa ggaagaaaac agttaaaacc tgtttgttga tggtttgctt
     1501 gatttgcttt tggtccaaac cgactttctg caagataata aagcgttcac ggtcttcgta
     1561 gccttcagaa atttgtttgt agtagatgac cagaacagtt ccgaccataa agataatgga
     1621 taggaaaata ccgataaaga agacaccacc aaagagggca ctcatctgag aactagcatc
     1681 tgctagattg ctaccataaa catagctacc ttctgtgtct aattgagcat taaattgatt
     1741 gaggtagttt tcatactcct cagcgacctt gagttgttct tcttcgctgg catctacatt
     1801 cataccaccg taaaactgat tatagatagc cgaatctggg aattgatcca aaaaggcttg
     1861 taaatcagga acaacaaggt aattgtaatc agtagtcaag atattaaact tatttggaac
     1921 atggttgaca atgaaatctt tattaaattc ttctttgaca gaaaattgat gatcatttag
     1981 agttagagtt ttctgtcctt tcagtccgtc atttttgaca aagagaccga cctcatttcc
     2041 tgatagagac agtttttgac cagtcatatt ttcataatct ttttggtcaa ataccatgaa
     2101 aactgttttt ggttggacac ggttttgtcc tttttcaaaa atagttaact tggttccttc
     2161 ttgattcgca acagcaaagt aagtgtaacg aagcacttct ttctctttaa tcttataacc
     2221 tttgtcactt gcaaactggc tcaagagttt gtccaaatct tctttttcaa cattttgccc
     2281 tgaaacccca aaatcatgag gatttagaac ttttttaaag gattctgcgg aattgaaaat
     2341 gcttgtcgct gctgacatgg ttaccaaaac cattgttgac aaaatagcga tagttgctag
     2401 tccaactgca tttttcttca tacggaaaat caggttggaa acagagatga ggttattagg
     2461 ttggtaatag tatttcttgt ttttcttcaa gatttgaaga aaaacggtaa tccctgcatt
     2521 gaacaaaaga taagtaccaa agataaccag taaaacagct atgaagaagg ttgttaaggc
     2581 tgtaagagga tcttttaccg taagggcaag ataatatcca atccctaaac ttatggaacc
     2641 aagaatagtt tggagaggaa ggaagcgacc ttttttctct ccgctagctt tctcacgtga
     2701 gagctggagg gcattcatac gggcgattcg aagggcattc aggaacatga ggcctaggaa
     2761 aatcaaaccg aagacaagaa gtactgtaat gacaactttc atctggaagg tagcgaccag
     2821 ttccaccttc aatttcatca gtttgagtag gaaagcgaaa atcaacttgt caaacaaggc
     2881 tccaataccg attcctgctc caacagttag aatcccaaat agcactaact ccttaaaggt
     2941 catactgatt agatggcgct tctccaagcc taacatgcca tatataccca gttccttgga
     3001 acggtttttc atgacaaaac tattggcata gaggacgata atggctgacg caagggtaac
     3061 gacaaacata ccaaatccaa gtgttgcttg aatggtggtt cctccacgga tttccgcaat
     3121 cttgggattg aaggttaggg agtaaaagag ataggtgatg gtgactgcca agagaacagc
     3181 cagtgcaaag ggatagtaga gcttgcggtt tttaatcaag ttcgataccg ctaacttatt
     3241 ggttaatcga aacatactaa ttcacctcgc ttgccatgac agtcaaggta tcagagattt
     3301 cttggaacat ctgacgctct gtcttctctc cacggtagat ttggttgtaa agaatgccgt
     3361 ctttgataaa gagaacacgc ttggccctgc tagcagctgc tgttgagtgg gttaccatga
     3421 ggatggtttg cccacgctca ttgatttcat taaagacatc aagtaaggct gcagatgact
     3481 tggaatcaag ggctcctgtt ggctcgtccg caaggagaat ttcaggttct gtgatgatgg
     3541 cgcgggctac tgctacacgc tgtttctgac caccagaaat ctcgtaaggg tacttctctt
     3601 gcaattggtt gatacccaga ttttcagctg tcatcaccaa tttcttcatc atctccgtta
     3661 taggtcttct tgacaagaca agcggaagca agatattgtc cttaacagac agagtatcta
     3721 gcaagttaaa gtcttggaag acaaatccta gcttttcacg ccggaaacta gaagcctgtg
     3781 aatttttaat agttgctgta tcagttccat ttaagtaaac ctgaccacga cttggtttgt
     3841 ccagcatagc taggatattg agaagagtgg atttaccaga accagactca cccatgatgg
     3901 caacatagtc acccttttct acggtaaagt gaatatcctt gagggcctct acttggttgc
     3961 cctgaaaacg tgttttataa attttttgaa cgtgttttac atctaaaagt gtcatgagaa
     4021 tctcctttct taatatccca tcaaatgaaa tgtagcttgc atcatagtgc atcccagctg
     4081 aacgcgctca atttctttgt gacttggttt tctagttttc ttatgtaaga tttgtttcat
     4141 gatgttactc ctttgttctt tatgagtcta gtttacatca aaaaaagacc gcttgccata
     4201 acctaacctt acaaaagaga ctttaatctt acatttttgt aagattgggt gaaatataag
     4261 caaaattact tagaaaatga tttgatttcc tatttttaaa gttgtaaaat ataagtgtga
     4321 aaaggcttac tttgaaaagg aggaagcggg aactaacatt ttagtaaggt tacctagata
     4381 acataccatt caatttaaca cttgcaagtc gttcaaccta taaggaaaga aggtgcgctt
     4441 taatgaaaca caaagaacat atactcatcg gacttctcta tctgctctct ccattcatcg
     4501 gtcagctctt agttgaacac acacacttta tcagtacaga atttaccggc actgcttatg
     4561 ttatttgctg gttatcagtt gttatcagca tccaccattt ttccaaaaat gtattgtctc
     4621 aacagcaaaa atagattaaa aaactgcgtt taaattactg cttttatagt aaaatgaaat
     4681 aagaacagga caaatcgatc aggacagtca aatcgatttc taacaatttt ttagaagtat
     4741 aggtgtccta ttctagttcc aatccactat agtttgtgga tgaagctggc gaaatattat
     4801 taatactatt caaaaatcaa agtgcaaact atatcaactt cacattgccg actaaattac
     4861 agcctgcggc tggcttccta gtttgcactt tgatttttat tgagtataaa agtaaaaaag
     4921 ctttaaatca agcagtttag actattgatt taaagctttt tgcgtattcc aaatcttttg
     4981 taaaacctca ccaaaaccag ccttcattat cgtcaatggc ttgagcttct ttgcgtttac
     5041 gtgggcctgt tccgtacatt tctgcttcga tttcttcctt ggttggcatg atagaagcta
     5101 ggatgatata gataatcacc ccaagtccaa agtttgcgac tgtaaaaatg gcaaacagaa
     5161 aacgtacaag ggtaacatca aagttccact tgtctgacag acctgctaaa actcctgaaa
     5221 tcatgcgatt tcgtctcatt ttataaaact tactgttcat gatatttcct ctttctgcta
     5281 ggttagacta gtatatcacg gataggctcg gctttcatca gtcctcaggc tgatttacta
     5341 atagcaactt tcctcgacaa agtccacagc gatagcgttt ggtatcaatc ctacgcttac
     5401 gctgataagt ttgctggcag gattggcaac gatagagctt gattggcttg gagttactat
     5461 tgggcaagga tggtacaaac cgtaatccat ccactgcttt caacagttcc ttaaaatccc
     5521 gatccttgtg ttgatagcct ttcccttgaa aatagaggtg ataatgacag agttcatgtc
     5581 ggacaatttt cctaaaaacg tccaacccca gttcctgata aaccttggga ttaaaatcca
     5641 aatgcccatc tttggggaaa aatcgcccac ctgtcgaacg tagacgccta ttccactgga
     5701 catgatggat aaaaggtctg ccgaagtctt ctagtgaaac ctgcttgacg taatcagtca
     5761 gtttcatttg gagctaggag agacagatta actttttcac gttcagtatc aattttctta
     5821 acccaaacgc tcaccaaatc tccaactgcc accacttgac tagggtgttt gataaacttg
     5881 cgactcatat gggaaatatg gatgagaccg tcctcatgaa ttccgatatc aacaaaagca
     5941 ccgaaatcaa caacgttacg caccactcct tctagctttt gtccaaccac taagtccttg
     6001 atatctagga catcttgacg gagaactgga gcatcaaagg aatcacggaa atctcgacct
     6061 ggtttgagaa gatctgcaat gatatcttta agagtttctg gaccaaggtc taactcttgc
     6121 gccatttcct tgactgaaag cgacttgagt ttgctttggg cctcttcatt cagatcctta
     6181 atgtccaagc atttgaagag ttccttaacg gcagcgtaat tttctgggtg aactcctgta
     6241 ttatcaagga tattgctact ttcagggata cggaggaaac cagcagcctg ttcaaaagcc
     6301 ttggccccca aacgaggaac tttcttgatt tgggagcgtg aagtgatttt tccctcttcc
     6361 tcacggtatt tgacgatatt ttcagaaatg gttttattga gtccagctac gtgtgaaaga
     6421 agagctgggc tagctgtatt gacattgaca ccaacttggt taaccactgt atcgacaaca
     6481 aagtccagac tctcagatag tttcttctga ctgacatcgt gttggtattg accgacacca
     6541 attgacttag gatcgatttt gaccaattcc gcaagaggat cttgcaaacg acgggcgata
     6601 gaaatggcag agcgtttttc aacggtcaag tctggaaact cctgacgagc aagttcgctg
     6661 gcagaataga cagaagcacc actttcatta acgataacat agctgacttc agggaaatct
     6721 ttcagaactt ccgctacaaa agcttcactt tcacgactgg ccgttccatt tccaatggca
     6781 ataatctcta caccgtattg accaattaaa tctgctaaat ctttcttggc ttcttcgatt
     6841 tgacgagctg atgctggttt aacaggataa ataacctgag ttgtcagcat ttttcctgtt
     6901 gcatccacga cagctagctt ggcacctgta cgaaaggctg ggtcaaaccc aagaaccacg
     6961 cgccctttca gtggagcaac caagaggaga ttgcgcagat tgtcagaaaa aagttggata
     7021 gctccctctt cagctttctc agttaattct gtccgaatac gacgctcaat agcaggcaag
     7081 acctttttct taacggattg ctgaacaact tcatcaatat aagcattttt caccttgaaa
     7141 cgagtagcaa agaaggcaag aatacggtcc gtcgcatgtt caaaaccgat cttcaagaca
     7201 ccaagtttct ccccacgatt gagagccaag gtacgatagc cttgcatagt tccaactgtc
     7261 tctgaaaaat cataataaat ctgaaaaacc tgcttttcat caagactttc atccttggct
     7321 tgagaagtga gtttagagtg tctcagcact tcctgataag tcatagcacg caatgtcaca
     7381 tcttccgata aggcttcgac caaaatatca actgcaccgg tcaaggcttc cttgccagtc
     7441 gcaaatcctt cacagacgaa cttttcagct tctttctcta agtcaactat attctgcaaa
     7501 atcaagcgag caagaggaaa gagtccagct tcacgggcaa tggttgcctt ggtacgacgc
     7561 ttttccttat aaggaagata gagttcttca acgtctgcta atttttcggc aactaagata
     7621 gcttcttcca attccttggt caacttacct tgttcttgaa tcttagctaa gacagcttcc
     7681 ttacggtcat tgagatttgt cagactttta tccaaatcaa taatagcctt aatcgccacc
     7741 tcatccagac taccagtcat gtccttgcga taacgcgcga taaagggaat agtcgcccct
     7801 tcagctgtca aacttagaac ggtatcaatt tgctttaacg tcactcccaa atcctgagag
     7861 attttttcat attttttatc cataaatcta ttataccaca agctggaagg ctttctccaa
     7921 tccttttcat actcaatgaa aatcaaaaag caaactagga agctagccgc aggctgctca
     7981 aaatactgtt ttgaggttgt ggataa
//
LOCUS       DBEROU010000065         7963 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.065, whole genome shotgun sequence.
ACCESSION   DBEROU010000065 DBEROU010000000
VERSION     DBEROU010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.065"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(116..391)
                     /locus_tag="ACL8CL_001972"
     CDS             complement(116..391)
                     /locus_tag="ACL8CL_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HGS1331152.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(498..1337)
                     /locus_tag="ACL8CL_001973"
     CDS             complement(498..1337)
                     /locus_tag="ACL8CL_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HGS1331153.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRISRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1479..2327
                     /locus_tag="ACL8CL_001974"
     CDS             1479..2327
                     /locus_tag="ACL8CL_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HGS1331154.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2420..3337)
                     /locus_tag="ACL8CL_001975"
     CDS             complement(2420..3337)
                     /locus_tag="ACL8CL_001975"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HGS1331155.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWS"
     gene            3816..3964
                     /locus_tag="ACL8CL_001976"
                     /pseudo
     CDS             3816..3964
                     /locus_tag="ACL8CL_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
     gene            complement(4290..4583)
                     /gene="rpmA"
                     /locus_tag="ACL8CL_001977"
     CDS             complement(4290..4583)
                     /gene="rpmA"
                     /locus_tag="ACL8CL_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HGS1331156.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4600..4944)
                     /locus_tag="ACL8CL_001978"
     CDS             complement(4600..4944)
                     /locus_tag="ACL8CL_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HGS1331157.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4960..5274)
                     /gene="rplU"
                     /locus_tag="ACL8CL_001979"
     CDS             complement(4960..5274)
                     /gene="rplU"
                     /locus_tag="ACL8CL_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HGS1331158.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5310..5397)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5456..6160)
                     /locus_tag="ACL8CL_001980"
     CDS             complement(5456..6160)
                     /locus_tag="ACL8CL_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HGS1331159.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKASF"
     gene            6562..6861
                     /locus_tag="ACL8CL_001981"
     CDS             6562..6861
                     /locus_tag="ACL8CL_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HGS1331160.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
     gene            7043..7963
                     /locus_tag="ACL8CL_001982"
                     /pseudo
     CDS             7043..7963
                     /locus_tag="ACL8CL_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2523 a         1626 c         1318 g         2496 t
ORIGIN      
        1 atacaatatt ttcggaacat tcaacttttt aactctattt attactagat ttcataatta
       61 aaaagcctac tgaccaagct agaaagcttg atacaatagg ctttttaaag actgattatt
      121 taacagcgtc tttaagagct ttaccagctt tgaatgctgg cactttagaa gctgcaattg
      181 tcatttcttt accagtttgt gggttgcgac ctttacgttc tgcgcgctca cgaacttcaa
      241 agttaccaaa accgatcaac tgaacttttt caccagctgc aagatagtca gctactgctg
      301 caaatacagc ttcaactgct gctgctgagt ctttcttagt caattctgta gcttctgcta
      361 ctttagcgat caaatcttgt ttgtttgcca tgttaatgat tcctccaaat aatttctaat
      421 taacaaatat aatcatatcc taaaatccct tttaggtcaa gttaaaaacg ctatttcttc
      481 ccattttatt tattttttta ggagtggtaa cgtatcaaaa tagcccaagc gttctcaccc
      541 gtgtgagttt gaataatgga acccgtttcc aaaacagaaa ttggcttttc aacataagct
      601 tgtaagcttt ctttcatctc ttttgcccaa tcatcactac cagaatatga aattccaatc
      661 tctgctacag cacgttcaga aagcgatgtt atcaactcat ctaaccattt tttaaatgtt
      721 ttagttccac gacctttaac cattggctgc aattcatggt ctttcatttg catgacaaca
      781 cggatattga gaagagagct caacaagcca gttacacggc taattcgtcc acctttgaca
      841 agattttcca aagttgaaac accaatataa agctctgtat ggtttttaac ctcttctaca
      901 tgagataaaa ttgcctccat atctttacct tcttgagcta acttcgcagc ctcaacaact
      961 tggaatttca gggcttggtc agtgaaggaa ctatcaacaa cagtcacatc tgcagtagat
     1021 aggctagcac cttggcgtgc tgcttctacc gtacccgaaa gagcatggga catatgaata
     1081 gcaagaatct ggccaccatc tttgcatagg tcttcaaaaa tctcagcaaa gacacctaca
     1141 ggtggctgac ttgttttcgg aagattctta ctttcttgca tcaactgaag aaatttacct
     1201 tcttctttca aatccgcatc agaataaaca acattatcaa tcattacaga taatggaaca
     1261 attgtaatat ctaattgctt tactagttca ggttcaatag taacagatga atcggttaca
     1321 atcttaattt ttgtcatagt atcaatcttt ctattttagg attcagattg gtttccttac
     1381 ttctaattat atcaaaaaaa agattaaaaa tcctaatgga gtcaatcaaa ttttccgtaa
     1441 aatttgatat aatcaactta taagaaaaga ggtgtcctat gattaaaaaa atttacccca
     1501 tttttaccat tttactaggt gctgctattt atgcttttgg actgacttat tttgtagttc
     1561 cccatcatct ctttgaagga ggggcgacag gcattaccct catcaccttt tatcttttta
     1621 aaatccctgt ttccctcatg aacctgctga ttaatattcc ccttttcatc ctagcttgga
     1681 agatctttgg agccaaatcc ctctattcta gtttactagg aaccttagct ttgtccgcct
     1741 ggttagcttt ttttgagcat attccccttc atattgatct tcaaggtgat ttactaatca
     1801 cagcccttat agcgggaatc ctattgggaa ttggccttgg aattattttt aatgctggag
     1861 gtacaactgg cggaactgat attctagctc gtattctcaa caaatacact catatatcca
     1921 taggaaaact gctctttatc ttagattttt gtattctcat gttgattctc ctaatcttca
     1981 aggatttgag attggtttcc tacacgcttt tgtttgattt tattgtttct cgtgttattg
     2041 atttgattgg tgaaggagga tatgccggca aaggctttat gattatcaca aaacgtcctg
     2101 accaacttgc taaggcgatt aatgatgacc tcggaagagg tgttactttt atttctggtc
     2161 aaggctacta tagtaaagaa aatttgaaaa tcatctactg tattgtcgga agaaatgaaa
     2221 ttgtgaaaac gaaggaaatg attcatcgaa tcgatcctca agcctttata actattacag
     2281 aagcccatga aatcctagga gaaggcttca cctttgaaaa agaataaaaa gaggtaatgt
     2341 cgtgacctca aaagttagac taaatcatct atcttttggg ttacagacaa cctctttttt
     2401 gttttattta ctcaagctct taagaccaat tccgagttac ttcttcatca gcctttaact
     2461 gatccactaa ttggtcaact gagtcaaatt tggtcatatc acgaatgcga tcaagccaat
     2521 aaaccatgac ggtttcccca taaatatctt gattaaaatc aaaaatattg acttcaaaac
     2581 gtgcttcttc tccatcaaag gtcacatttt tcccgacact agccatagca cgatacttct
     2641 gtctttgaat ctcaacatca acgacataaa cgccatctgc tggcatataa gtacggtcta
     2701 aaagcactaa attcgctgtc ggataaccaa ttgtacgacc acgagcatta ccatgaacca
     2761 ccatacctct tgatggaagc ggtgccccca aaagttttcc tgcttctttc acatttccat
     2821 ctaaaatagc ttgacggata cgagttgaac taatctttcc tttctcatct tctacaggtg
     2881 gaacaatgat aacttctcca tcaaagtaat cctttaaatc ttctgctgtt tttttgtcag
     2941 aaccaaatgt ataatcaaaa cctgcaacaa taattttggc attcatagcc ttgatataag
     3001 ttgcaaagaa ttcttgtgca gtgagactag cgaattgact actaaaatca aggagatata
     3061 attcttctac accttcgcgc tttaattttc tttcacgttc agcagggttc aaaatatgca
     3121 aaaacaaatc tggatgataa ggctctaaag cgatctttgg agattcatta aaggtcataa
     3181 cgacgatagg caataaatcc tttctcgcag ccttgttggc aacacgaaat aattcttgat
     3241 gccccttatg tatgccatca aaatagccga gaacaacgac tgaatcagat ggtgtgccaa
     3301 tatctttttg gttttttata ggaatagtaa taatcataaa ataattatat catagcgata
     3361 gctatttctg gaacagaaaa tctgaaatgt tgtttttttc acatgaagtg tacctgtttt
     3421 caaaaagcac tttattctat cgttgcttaa ctatgaactt tgcaatattc ttctcaaaaa
     3481 cttgtaggac atcttcaaaa ttttgcaagg agtgattaga cttgttcggt aaccataaag
     3541 tgtcatacta tgttatgtat gaaaaagcaa tgcaactaac tcctgagaac tttaaattac
     3601 taattggtgc cgaaaaggta gaatttagaa tcgaggtaca cctatggctg taaaatttac
     3661 aaaatgagac aacttgggca agatgtttga agaatttcct aaactccctg atttgaagca
     3721 agtcactttc cctgatgaca aagaaaaaag ccaaaacagc aaagaaaaac tagatgactg
     3781 ctttccaaca actcccatct agtgtgcttc agactgggct atttttctct ccatctgtta
     3841 gcttggattc tcagaccgtt tcagctaaag aatatctttt cccttatcag aaggaacggc
     3901 tcaaaccatt cagacaagtg aagggatgac aagccaatat ttgaaaccag atagcagttc
     3961 ttatagtcaa ttgaaataaa atctgaagaa atcgagtagg aaactcatat caatttttaa
     4021 cagtgttcta ttccagattc atactcaatg aaaattaaag tgcaaactag gaagttagcc
     4081 gcaggctgta cttgagtacg gcaaggtgaa attgacgtgg tttgaatttg attttcgaag
     4141 agtatcaatc cactatacat attaaaaagt atgggtatca aaataaaaaa acaggctctc
     4201 cgaaaactcg gaaagcctta ttttaagctg tttctagctt cctcaccttt acatttcaag
     4261 gttcgggata aaaggttcaa tgaacctttt tattttgcga ttgggtaaac agaaacttgt
     4321 tttttatcgc gtcctttacg ttcaaagcgt actacgcctt caactttagc gaacaaagta
     4381 tcgtctccac cacgaccaac gtttacacct ggatagatgt gtgtaccacg ttgacggtaa
     4441 aggattgatc cacctgttac agtttgtccg tcagctgctt tagctccaag acgttttgct
     4501 tgtgaatcac gtccgtttga tgtagaacct ccaccttttt tgtgggcgaa aagttgcaag
     4561 ttgttaagag tcatttttaa cataatgttt tcctccgtgt tagttttctg tgataactct
     4621 ggtttggacg aactcagaag agttctccga taagtttgcc atacctaaga aaaatgattc
     4681 aaagaataac tgggtcattt ctctctggtg tgaaggaaga tcttttggta tttcaaccat
     4741 cagatagcca ccttcatctt cgtttaattc taggattggt tcatagcctg caaatttctc
     4801 aatagaattg ataaagttaa tggcaagcgt agaaaccgat gcacacacga catctaagcc
     4861 gtattcgcca ctctcggcgt gtccagtaat ttccgcactc ctcagctcgc catcttcggc
     4921 tctctcaaag actgcctgta tcatgtgttc tccttaaaat taagcgttga ttgcgttgat
     4981 gacaactttt gtatatggtt gacggtgacc ttgtttacgg tggctacctt ttttaggttt
     5041 gtacttgtaa gtaaccactt tcttttgttt tccttgtttt tcaacagttc caactacagt
     5101 agctccagca acaagtggag ttccgacaac agtgttttca ccaccaacaa gaacaacttc
     5161 gttaaaagta acttcttgac cagcttcaac gttcaatttt tcaacgtaaa ctgcttgacc
     5221 aacttcaact ttaacttgtt ttccgccagt tttgataatt gcgtatgtgc tcattatgca
     5281 cctcctatga tttttatggg tttccccgat tttttgtgaa gactcgccta gcatcgtggg
     5341 acgaaccact taaacttgaa taatcaagca acgatgttcg tgcggttgca caggatcgtg
     5401 catagtcaac tcttcaagta tagcatatct cctattttct tacaagtaat aacacctaaa
     5461 atgaagcttt ttcttttact tttttctgcc aagaggcaaa aagcatgctg aggtaaaaaa
     5521 cgctcatcat aataggaaca ccaagaatgg tcttttcatg atagaaaatc gtcaaatagg
     5581 ctgaaaagac aacgccaagg acaaaactac taagcaggct aacaaatatg aatccttcac
     5641 gcaaaaaagg agtgtgcttg gttcggaaat aatctccaaa agccagcatg gtccgtttga
     5701 tattccctgt cataaaagcg ttattatagg caatacccga cacttctcca aaagcagttg
     5761 tcaccagtcc catacagaag gccaagggcg gcactagata gatattatcc acagtttgcg
     5821 gcacaaaagc aataatgatt gataagattg ccaagggaat caaggacaga ataggttttt
     5881 tcacaattct caatttttcc ttataaatcg ttaataaaaa gactcccatc ataaacgcta
     5941 gcaaggtgag aaccttgtcc ctaacatccg aaacattatt tttaattaat tctactgaaa
     6001 gaaagacaac atttccagtt tgtccagcta caagggtatt cccgcgaaca ataaaagtgt
     6061 aagcatccac atatccagca caaaacgtca aaaaaagtgc taacctttta gactgacgtg
     6121 atatttttct tataggtaat aacctcattt tacctcccat tgtattttct cttagaaata
     6181 ttgtaccatt ttctttctaa aaaatcgtag gctaccattt agattttact attagcataa
     6241 aaataataat agacaactat ttatccaaaa atagatagat gtaacatgtt tgcaaacaaa
     6301 gcatacgaac ctttagtaaa atcatttcca tgaaactaga atagagccct cttagcaaaa
     6361 atcattattt taatttattt ctaatcactc cttgacataa ataactctca ccaataaaag
     6421 actatgtctt aaaaaaatgg tataataaaa tcaatacttg ggcttgatgg ctatgctact
     6481 aataacaatt aggagagaaa atcaggcact tgttaacaac aaggattatc cccttgagat
     6541 gaaaggaact ttagaaatct tatgatgaac atgcaaaaca tgatgcgtca agcacaaaaa
     6601 cttcaaaaac aaatggaaca aagccaagct gaacttgctg ctatgcaatt tgttggcaaa
     6661 tctgctcaag atcttgtcca agcgacctta actggcgata agaaagttgt cagcattgat
     6721 ttcaatccag ctgtcgttga cccagaggac cttgagactc tttctgatat gaccgttcaa
     6781 gccatcaact ctgctcttga acaaatcgat gaaactacca agaaaaaact gggtgctttc
     6841 gctgggaaat tacctttcta aaaacaagga gctagaacaa tgcttgtcga taacaaaggc
     6901 taagaaaggt gcaaaaatga ctctataata tttgtagtgg gtaaatccac tatagatatt
     6961 atggagccta ttttattgta gaaaaaaagt cccatatgac ctataatgaa aagcgacaaa
     7021 acaactcatt agaaagaatc atatggaaca attacatttt atcacaaaat tactagacat
     7081 taaagaccct aatatccaga ttttagacat catcaagaag gatacacaca aggaaatcat
     7141 cgccaaactg gactacgacg ccccatcttg ccctgagtgc ggaaaccaat tgaagaaata
     7201 tgactttcaa aaaccttcta aaattcctta tcttgaaaca actggtatgc ctactagaat
     7261 tctccttaga aagcgtcgtt tcaagtgcta tcattgttct aaaatgatgg ttgctgaaac
     7321 ttctatcgtc aagaagaatc accaaatccc tcgtattatc aaccaaaaaa ttgcgcaaaa
     7381 gttaattgaa aagatttcta tgaccgatat tgcccatcag ctttccatct caacttcaac
     7441 tgttattcgt aagctcaatg attttcactt taaacatgat ttttcttgtc ttcctgagat
     7501 tatgtcttgg gatgagtatg cttttacaaa aggaaagatg agcttcattg cgcaagattt
     7561 taacaatctc aatatcatca ctgttcttaa gggaagaaca caagctgtca tccgaaatca
     7621 ctttcttaaa tatgatagag ccgtccgatg tcgcgtcaaa atcattacta tggatatgtt
     7681 tagtccttac tatgacttag ctagacagct tcgctttcaa atttctaggc tcaggctgaa
     7741 acagtctccc agactgtttc actcccgaat gctaaaatcg ttctagatcg tttccatatt
     7801 atccaacatc tcagccgtgc tatgagtcgt gtgcgtgtcc aaatcatgaa tcagtttcat
     7861 cgaaaatccc atgaatacaa ggctatcaag cgctactgga aactcattca acaggatagc
     7921 cgtaaactga gtgataagcg attttatcgt cctacttttc gca
//
LOCUS       DBEROU010000066         7501 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.066, whole genome shotgun sequence.
ACCESSION   DBEROU010000066 DBEROU010000000
VERSION     DBEROU010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.066"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(48..1901)
                     /locus_tag="ACL8CL_001983"
     CDS             complement(48..1901)
                     /locus_tag="ACL8CL_001983"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HGS1331161.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            complement(1914..3173)
                     /gene="rseP"
                     /locus_tag="ACL8CL_001984"
     CDS             complement(1914..3173)
                     /gene="rseP"
                     /locus_tag="ACL8CL_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HGS1331162.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            complement(3195..3998)
                     /locus_tag="ACL8CL_001985"
     CDS             complement(3195..3998)
                     /locus_tag="ACL8CL_001985"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HGS1331163.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            complement(4007..4765)
                     /locus_tag="ACL8CL_001986"
     CDS             complement(4007..4765)
                     /locus_tag="ACL8CL_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HGS1331164.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKYLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEVALQEAILAYNRRHRRFGGV"
     gene            complement(4996..5568)
                     /locus_tag="ACL8CL_001987"
     CDS             complement(4996..5568)
                     /locus_tag="ACL8CL_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HGS1331165.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLEKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTTVGLRLNEESDFELYAPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            complement(5549..6547)
                     /gene="ruvB"
                     /locus_tag="ACL8CL_001988"
     CDS             complement(5549..6547)
                     /gene="ruvB"
                     /locus_tag="ACL8CL_001988"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HGS1331166.1"
                     /translation="MSRILDNEIMGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEA
                     AKMRDEALDHVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELE
                     PGDVLFIDEIHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTR
                     AGMLSNPLRARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPR
                     IANRLLKRVRDFAQIMGNGVIDDVITDKALTMLDVDHEGLDYVDQKILRTMIEMYGGG
                     PVGLGTLSVNIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            6681..6773
                     /locus_tag="ACL8CL_001989"
     CDS             6681..6773
                     /locus_tag="ACL8CL_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HGS1331167.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
BASE COUNT         2256 a         1747 c         1371 g         2127 t
ORIGIN      
        1 taaaaactag ttacattttt tccttgtttt tcttctgatt atagttttta tttcttgctt
       61 aggatttcaa gcgtttcaag cacgttatct gcatgaacct caatggtgtc accagttgcc
      121 ttgatcttaa cttctacaat gccatcggcc gcttttttac caacagtgat acggattgga
      181 agaccaatca agtcactatc gctaaattta acaccgacac gttcgttacg gtcatctgtc
      241 aagacttcat aaccagctcc catcaagctt gcttcaagtt tttctgtcaa ggcttgcgct
      301 tcttcatcct tgacattgac agtaatcaaa tgcacatcaa atggtgccaa ttctttaggg
      361 aaattgattc cccaagcgta acggtattca ccttttggcg ttttgttaac aaagaggcga
      421 gcgtgttgct ccatcactgc tgaaagaaga cggctgacac cgataccgta acatcccatg
      481 atgattggca cagcacgacc attttcatcc aagacatctg ctcccatgct tgctgaatag
      541 cgagttccga gtttgaaaat atgaccgatc tcaataccac gcgcaaagtt aaggacacct
      601 tgtccatctg gggaaatttc accctcacga acttcacgga tatccacata ttctgcagta
      661 aaatcacggc ctgggttcac accagtcaag tggtagccat cttcgttagc accgacaact
      721 gcattgcgaa catcttgcac cttacgatct gcaataattt taatattctc tggcaaacca
      781 actggtccaa gtgaaccaaa tcctgcttga acaacattcg ccacttcttc ttcgctagca
      841 acgtcaaaga aatctgctcc taagtggttt ttcaacttga cttcgttgag ttggtcattt
      901 ccaactagaa gggctgcaac aagctcacca tctgcaatgt agaagagggt tttaatcgtt
      961 tgttcttctg gaacattgag gaaggctgca acttcatcaa ttgatttaac atctggcgtt
     1021 gcaacacgag taacttcttc ttcagcgaca acacggttgc ttggtttgta ctcgtttgtt
     1081 gccatttcta agttagctgc atagctagac tcacttgagt aagcaatggt atcttcacca
     1141 gagactatcc atttgagcaa ttctgccttg atttcttctt gcacttctgc aggaatttcg
     1201 tcaaatgagg caactgactt gtccaagaca acccagcggt caaggtctgt acgagcagat
     1261 gtaatggcca taaattcttg gctatcctta ccacccatgg ctccaccgtc accaataata
     1321 gccttgaagt ctaaaccact acgagtgaaa atacgctcat aagctgcttt gtactcatca
     1381 taaacactat ccaaactatc atagttagcg tggaaactat aagcatcctt catgataaac
     1441 tcacgtgtac gaagaagtcc attacgcggg cgtttttcat cacgatactt gggctgaatt
     1501 tgataaaggt tgagtggcaa ttgcttgtaa gatttaacag aatcacggac aatagctgta
     1561 aaggtttctt cgtgagttgg acctaagata aagtctgatt tttcacggtt ttttagtttg
     1621 taaaggtctt caccataggt ttcgtaacga cctgattcac gccacaattc tgcactaaga
     1681 agggctggag ccaacatctc aacagcacca atcttttcga attcttggcg catgatgttt
     1741 ttagcttttt caatcacacg gttggcaagt ggtagataag aataaacacc tgctgaaact
     1801 tggcgaacat aaccagcacg caacataaga gcatggctga taacttgagc atcgcttggc
     1861 atttcgcgaa gcgttgggat aggcatttta ctttgtttca taatattcct cgattatcta
     1921 aaaaagagtc gcataatgtc attccaagtc acagcaatca tcaagacaac catgatgacc
     1981 actccggcca aggtgacata ggtttcaatt tcttgtttca atggtttgcg gcggatggct
     2041 tctaggatat tgagcacaat cttaccacca tccaaggctg gaatcggaat aagattaaaa
     2101 atcccaatat tgatggaaat cattgccaag aagtacaaga tattctcaat tccattttta
     2161 gcagcatcac tacttgcctt aaagatagca acaggtccac ccaacttgtt caaatccggt
     2221 tggaaaatca gatttttcag agctgagaga attcggagag ctgagtcagc agcagttgta
     2281 aaaccaccta caaacatgga tagaaaatct gacttaaccc ccggttgaac acctagaagg
     2341 taacgacctt gactatcttc gggtgtaaca gtgacttgtt tgtcactccc cttttcagaa
     2401 atagtcacat ccaaagtcgg tgccgtctta tctttggttt ctgtttccac agcttggatc
     2461 aagctttccc agttgctaac ctcatgtgag ccaatcttgg taatttgtgc cgtttctggt
     2521 actcctacct tggccaaggc accttggggc atgatatgga actgattggt atcaacatct
     2581 ctgacaccac cctgcataaa gattaaaacc caaaaaacaa cgacacctaa gataaaattg
     2641 ttcataggac ctgcaaaatt ggtaatcagt ttgccccaga tagtcgcatt ttgatattga
     2701 acatctaaag gtgcaatccg aacctcagta ccatctgctt ccacaaccgt tgcatcgtga
     2761 tccactgcaa atgttttttc ttcttccaga accaatcctt tgataaagag cttgtcttca
     2821 aaatcaaact gggtcacctg catagggagg gctgtttgat ccaatttttt acctgagaga
     2881 ttgatgcgtt taaccttacc atcatcagca agtgtcaaac taacaggcgt tcctgtcttg
     2941 atttcagttg tatcatcacc ccaaccggcc atgcggacat agccacccag aggcaagatt
     3001 cgaatggtat aggccgttcc atccttgcca atgtgagcaa aaattttagg tcccataccg
     3061 atggcaaatt cacgtactaa aatccctgat ttcttggcaa agtagaagtg cccgaactcg
     3121 tgcaccacta caataatccc gaaaaccaga ataaaggtta aaattccgag catagcgttt
     3181 cctccgtctt ttgattaaaa gagtccaaat aagtgcatga ttggaaatac aagcaacata
     3241 ctatcgaaac gatccaaaac accaccatgt ccagggataa atttcccaga atccttaaca
     3301 ccaaaatgac gtttgatcga actttctagt aaatcaccaa attgtccagc aatgctaaag
     3361 aaaatagcaa agactgacat cttgtaaatt ccatatggaa gagcaactgt actgtcaact
     3421 atcataaaga taatggttac taaaattgct cctaaaatac cacccaaggc accctcaagg
     3481 gttttattag gcgataccct tggtgctaac tttcgtttcc catagttcat cccaacaaga
     3541 taggcaccac tgtctgtcgc ccagacgata cacaaggcta agagagcctt gtccaaacct
     3601 gcaacacgag catctagtaa agcattaaat ccaaagccca cgtagaagct catagcaaga
     3661 gggaaaaccg catcctcaat cgtataagac ttgctaaaaa cggtcgttcc taacatgatt
     3721 gaaatcaaaa cactataggc aaccacattc ccatcaactg gcaaaaaagt caggtaattc
     3781 tccaagggaa tggtcaatgc aaaggttgca aagagggtca agaggccctc catcgtcatg
     3841 gtctctagac ctctcatctt caaaagttca tgcatggcta gcatggctat gattccgatt
     3901 gctatctgaa gcaagaggcc cccaatcatt aaaattggta ggaaaatagc cagggcaatc
     3961 cctgcaaaca aggttctttt ctgtaaatcc tgggtcatat ttcctcctaa actcctccaa
     4021 atcggcgatg acgacgatta taggcaagaa tagcttcctg caaggccact tcgtcaaaat
     4081 caggccataa ggtgtccgta aaataaagct cactataggc tccctgccat ggaaggaaat
     4141 tgctcaaacg taattctcca ctagtacgga taatcaagtc tgggtctcgt aagtacttag
     4201 gcaaatgctg ggtaaagaga tagttgccaa tcaattcctc tgtgatgtca cctgggttga
     4261 ttttggcatc taaaacatcc tgggaaatca acttaagcgc ctgtgtaatc tcagcacgtc
     4321 caccatagtt aagagcaaaa ttaagaatca atcctgtgtt gttcttagtc aattcctcag
     4381 ccttggttaa agcttcaaag gtttgcttag gcaggcggtc tgtctcccca atcatttgaa
     4441 tcttaacatt attcgcatgt agttccggga cataattatc ataaaactct actggcaagt
     4501 tcatgataaa cttgacttcc tgatctggac gggtccagtt ttccgtagaa aaagcataga
     4561 ccgtaataac cttgacgccc agtttgttgg ctgccttggt cacggtttgc aatgcttcca
     4621 tgcccgcctt atgtccaaaa actcgcggtt gcatacgttt tttagcccaa cggccattgc
     4681 catccatgat gatgccgata tgagcaggaa cctgtgtcgg aacctctact tccacagcct
     4741 tatctttctt aaaaaatcca aacatgatct tattcctatt caaaaatcta tcgtttcatt
     4801 ataccatatt tccccatttt cttctatcac taagctattt attctcaggc accaagccca
     4861 tttttcaaaa aaataagccg cctgattggg cgactttatt tttataggga gattattatg
     4921 aaaaagtttt aggagtttaa gttaaggtct tcttaactta tgaacttagt gtacactccc
     4981 tagcttaaag tttccttaag tatttttaaa aatcaaattt ttccatttct cctgccaatt
     5041 tttcttggat aaacgtgttt gatagagttc cattcggtct tcattttcta agaaatgagg
     5101 agttggacga acttgaaaat tcaaaatatc ctccaaacca taaggtgcat agagttcaaa
     5161 atctgattct tcattcaagc gcagtccaac tgtcgtacac cgttctggat acttactcat
     5221 agcatcacga gaactggtat aggaagcagt gtgaggactg tgctgatgca tatagacctg
     5281 atttttcaat tcccactggt actgaggaaa atcctctcgc agctttttct ccagtaataa
     5341 ggtttcctca taagaaaaat ctggatcaaa gaaaatcaca tctatatctg tttcatgatc
     5401 aaaaggggat ttgtctgaca aaagattcca aatgaaattt ctgacagaac ctgctgccaa
     5461 ccacgagtct ttcagaccaa ggtctcggat gatcgtcaga atggccatca tatctggatt
     5521 ttctctaaaa gcctctaaga tttcttgctt atttttcact gtattcataa cctaagtgct
     5581 catatgcctt agcagtcgcc acccgtccag accgtgtccg catgataaaa cctttttgaa
     5641 tcaagtaagg ctcatacatg tcttctacag tctcacgctc ctcggcaata ttcacagaaa
     5701 gagttcctaa tccgacaggg ccaccaccgt acatctcaat catggtgcga aggatttttt
     5761 gatccacata gtccaaacct tcatggtcaa catccagcat agtcaaagcc ttatcggtaa
     5821 taacatcatc gataacccca ttccccatta tctgggcaaa atcgcgcacg cgcttgagga
     5881 gacgattggc aatacgaggg gtcccacgac tacgtagggc caactcagat gctgcctcat
     5941 gggtgatttc catctcaaaa atatctgccg tccgctcgac aatttctgtc aaatcagcat
     6001 gagcataata ctccatatga cctgtaatcc caaaacgtgc ccgtagcgga ttggagagca
     6061 taccagcccg agtcgtcgca ccaatcaagg taaaaggtgg taactccaaa tgaacactac
     6121 gactaccctc accagcccca atcataatat cgatgtagaa gtcctccata gcactataaa
     6181 gcacctcttc cactgacatt ggcaaacgat ggatctcatc aataaaaagt acatccccag
     6241 gctctaactc attcaaaata gctaccagat ctccggcttt ttcaatgact ggacccgacg
     6301 tctgcttaag attgactccc agttcgttgg caataacaaa ggccatggtc gttttcccga
     6361 gacctggagg cccaaataag agcacatgat ccagcgcttc atcccgcatt ttggcagctt
     6421 caataaagat ttgtagctgg tccttgacct tatcctgtcc gatatattca cgtaaatact
     6481 gaggacggag cgtgcgttct actaactcct catcccccat tatctcatta tctaaaattc
     6541 tactcatggc tctattatat caaaaaacca agccacaaac aaaaaagcca cctgattggg
     6601 tgactcctaa gtttagcact tatgtggtat aatattctac gacactgcta caccgcctac
     6661 gaaaggaggt gagatagccc atgatggaat tagtactcaa aactatcatc ggaccaattg
     6721 tggtcggtgt cgttcttcgt atagtcgata aatggctaaa caaggacaaa tagtgtcaaa
     6781 aaagacctca agcttatttg gtcgtgagct tggggtcttt tctagcctat gatatagaac
     6841 tagtactcaa ttccttttta ttatcccata gttcacgaat tttgtcaaag gtttacattt
     6901 tcttcaaccg ctgtacgaca agacggttaa gattaagaga acgttaggga ttctatcaat
     6961 ttcatagaaa ttttgatttc gtaaacgaag agacaatctt acatgtcact tctcatttaa
     7021 tacgccacta ctagacaagc aaaatcatta ttacagtagt tccagttctt caattaacag
     7081 tcacttacaa tcaaattgag tttgaactag ctgaagcgac cacagaccta tttcttagtt
     7141 atattcgcta aaaaagtccc cgccaattcc ccgaccaaaa tccgaaaaat accgaaaaat
     7201 atcgaaaaat tatttttaga atagtcccca aaagcctgaa atagagctaa aaaactccac
     7261 ctgattgggt ggagttaagg gagattatta tgaaaaagaa aagtttagga ttttattaaa
     7321 taaagttagg aggtctttat ttaataacta catgatacaa gacgaaactt aaaactacct
     7381 taacttttct aaaattttac tattttgcaa aaaatttcta tcaccagcac ctcaccacgc
     7441 aaaaaagcca cctgattggg tggcttcatt aggagattgt gatgaaaaaa gtttaggatt
     7501 t
//
LOCUS       DBEROU010000067         7251 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.067, whole genome shotgun sequence.
ACCESSION   DBEROU010000067 DBEROU010000000
VERSION     DBEROU010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 7251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7251
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.067"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            7..453
                     /locus_tag="ACL8CL_001990"
                     /pseudo
     CDS             7..453
                     /locus_tag="ACL8CL_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            514..1476
                     /locus_tag="ACL8CL_001991"
     CDS             514..1476
                     /locus_tag="ACL8CL_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HGS1331168.1"
                     /translation="MNMSNITIDGQEMDLSQTMPSYAQVVSLYDDTSISVSNNETDKV
                     LAGSLYTDTNEQGLTIPSSLLKNWNEQTGKNLTANDLIGKSVSASIVESAAETSKIAQ
                     FQTKIVRVINDEDDMEDSNSFMLSHQMETILKEAGFTKAVSYFILELKDPSQTKVVTE
                     ELQKNKKYTVLSQQRVLDIVITFIRVIQGLLIVLSSQAIVVAAVMIGIIIYINIMQRS
                     KEIGVMKAVGYQNRDVKGIFIYEAIWIVGIALLLAFLVAQGVGSLANAIVSHFYPSIT
                     KVFELNLLSVLGTLVFALLLGYVSAYFPARKISKMDPIESLCYE"
     gene            1653..2660
                     /gene="gap"
                     /locus_tag="ACL8CL_001992"
     CDS             1653..2660
                     /gene="gap"
                     /locus_tag="ACL8CL_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HGS1331169.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2728..3603)
                     /locus_tag="ACL8CL_001993"
     CDS             complement(2728..3603)
                     /locus_tag="ACL8CL_001993"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HGS1331170.1"
                     /translation="MQFTFTLPASLPQMTVKQLLEEQLLIPRKIRHFLRIKKHILINQ
                     EEVHWNEIVNPGDVCQLTFDEEDYPQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTH
                     GNQPNEIALLNHVSTYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISR
                     EYWALVDGNINKKELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLA
                     HCKLKTGRTHQIRVHLLHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLERLTF
                     TTLSNTFEKELKKNG"
     gene            3690..5885
                     /gene="pbp2a"
                     /locus_tag="ACL8CL_001994"
     CDS             3690..5885
                     /gene="pbp2a"
                     /locus_tag="ACL8CL_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP2A"
                     /protein_id="HGS1331171.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENTSLFPRAEDGTSAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYTVDNYAGI
                     KTSREVPMYQALAESLNLPAVATVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIANHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYVMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTPGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5938..6090
                     /gene="rpmG"
                     /locus_tag="ACL8CL_001995"
     CDS             5938..6090
                     /gene="rpmG"
                     /locus_tag="ACL8CL_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HGS1331172.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            6100..6276
                     /gene="secE"
                     /locus_tag="ACL8CL_001996"
     CDS             6100..6276
                     /gene="secE"
                     /locus_tag="ACL8CL_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HGS1331173.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            6331..6867
                     /gene="nusG"
                     /locus_tag="ACL8CL_001997"
     CDS             6331..6867
                     /gene="nusG"
                     /locus_tag="ACL8CL_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HGS1331174.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTVQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
     gene            6989..>7251
                     /locus_tag="ACL8CL_001998"
     CDS             6989..>7251
                     /locus_tag="ACL8CL_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HGS1331175.1"
                     /translation="MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISR
                     EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRKYDKEPYE"
BASE COUNT         2279 a         1245 c         1573 g         2154 t
ORIGIN      
        1 gtttagattg acgaaacagg attcgatact tatttttatc gagaatatgg tcgctcatta
       61 aaaggtcagt taataagagg caaagtatct ggaagaagat atcagaggat ttctttggtt
      121 gcaggtctaa caaatggtga gttaatcgct ccaatgactt acgaagagac gatgacgagc
      181 gacttttttg aagtatggtt tcagaagttt ttcttaccaa cattaaccac accatcggtt
      241 attattatgg ataatgcaag attccataga atggggaagc tagaactctt gtgtgaagag
      301 tttgggcata aacttttacc tcttcctccc tactcacctg agtacaatcc tattgagaaa
      361 acatgggctc atatcaaaaa gcacctcaaa aaggtattac caagttgcaa taccttttac
      421 gaggcttttt tatcctgctc ttgtttcaat tgactatata tcacggatac agttgggaaa
      481 gaagctatta aatatttgga gagtcctttt gggatgaaca tgtcaaatat tactatagat
      541 gggcaagaaa tggatctgag tcaaacgatg ccttcttatg ctcaagtagt gagtctttat
      601 gatgatacaa gtatctctgt tagtaataat gagacggaca aagtgttggc tggatccctc
      661 tatactgata caaatgaaca gggattaacg attccaagca gtttactaaa aaattggaat
      721 gaacagacag gaaaaaattt gacagctaat gatcttattg gcaaatcagt ctcagccagc
      781 attgtagaaa gtgctgccga aactagtaag attgctcaat ttcaaacgaa gattgtacgt
      841 gtaatcaatg atgaagatga catggaggac agcaacagtt tcatgctgtc tcatcaaatg
      901 gaaacgattt tgaaagaggc tggatttacg aaagctgtat cttattttat cttggaactc
      961 aaagatccat cacagacaaa agtagtaaca gaagaattac agaaaaataa gaagtatact
     1021 gtgctttctc aacagagagt tcttgatatt gtgattacct ttattcgtgt tattcaggga
     1081 ttattgattg tgctttcatc acaagctatt gtggtagcag cggttatgat tggtatcatt
     1141 atttacatca atatcatgca acgttccaag gaaataggtg tcatgaaagc agttggttat
     1201 cagaatcgtg atgtcaaagg aatttttatt tacgaggcta tctggattgt aggcatcgcc
     1261 ttgctgctgg catttttggt tgcacaaggg gtgggaagtt tggcgaatgc gattgtaagt
     1321 cacttttacc catccatcac taaggttttt gaattaaatc ttttatctgt tttaggaact
     1381 ctagttttcg ctctattact tggttatgtc tcagcctact tcccggcgcg taagattagt
     1441 aaaatggatc ctatagaatc gttatgctat gaatagtaag ggattccttg gtttacagtc
     1501 ttaaaaagaa actaacatag aaaaacaatg gccatacttg caattcaact caaatagttg
     1561 tataataggt ttgttgaaag gtgatttgta gtgattcaag ttactctttt cacaataaaa
     1621 atttcatgat tttcataagg aggaaatcac taatggtagt taaagttggt attaacggtt
     1681 tcggacgtat cggtcgtctt gctttccgtc gtatccaaaa cgtagaaggt gttgaagtta
     1741 cacgcatcaa cgaccttaca gatccagtta tgcttgcaca cttgttgaaa tacgacacaa
     1801 ctcaaggtcg tttcgacggt actgttgaag ttaaagaagg tggatttgaa gttaacggta
     1861 aattcatcaa agtttctgct gaacgtgatc cagaacaaat cgactgggct actgacggtg
     1921 tagaaatcgt tcttgaagct actggtttct ttgctaagaa agaagcagct gaaaaacacc
     1981 ttaaaggtgg agctaaaaaa gttgttatca ctgctcctgg tggaaacgac gttaaaacag
     2041 ttgtattcaa cactaaccac gacgttcttg acggtactga aacagttatc tcaggtgctt
     2101 catgtactac aaactgcttg gctccaatgg ctaaagctct tcaagacaac tttggtgttg
     2161 ttgaaggatt gatgactact atccacgctt acactggtga ccaaatgatc cttgacggac
     2221 cacaccgtgg tggtgacctt cgccgtgctc gcgctggtgc tgcaaacatc gttcctaact
     2281 caactggtgc tgcaaaagct atcggtcttg taatcccaga attgaatggt aaacttgatg
     2341 gatctgcaca acgcgttcca actccaactg gatcagttac tgaattggta gcagttcttg
     2401 aaaagaacgt tactgttgat gaagtgaacg cagctatgaa agcagcttca aacgaatcat
     2461 acggttacac agaagatcca atcgtatctt cagatatcgt aggtatgtct tacggttcat
     2521 tgtttgacgc aactcaaact aaagttcttg acgttgacgg taaacaattg gttaaagttg
     2581 tatcatggta cgacaacgaa atgtcataca ctgcacaact tgttcgtact cttgaatact
     2641 tcgcaaagat tgctaaataa ttcttgagtt gatagaaagc aaggctttgt ggtcttgctt
     2701 ttttatatgg aaaaatggat gacacgatca tccattcttt tttaattctt tttcaaatgt
     2761 atttgaaagg gtagtgaaag ttagcctctc taaagtaagt gggtgggtaa aggaaagtcg
     2821 gaaggcatga agcataagcc ggcttgtctt tgatttacta ttatagagag ggtctcccag
     2881 gataggaaga ttatgatgca aaaggtgcac acgaatctga tgggttcgcc ctgtctttag
     2941 cttgcaatga gccaaggaag tcttgtttga gaattgcttt aatctgctta catgcgtttc
     3001 agcatattgc ccattttttg catcaactat tctttttcta cgatcatggc gatcacgtcc
     3061 aattttgtct ctgaaaacaa gttctttttt gttgatattt ccatcaacta gagcccaata
     3121 ttctctagaa atctcttttt tctccaataa gcgattgaga atgggcagga taaaaggatt
     3181 tttggcaaag agaactaagc cactggtttc catgtccaga cgatgaacga catagcaggt
     3241 ttggccaaca taggtactaa catggttaag aagggcaatt tcgtttggtt gattaccatg
     3301 cgttttcata ccctctggtt tgtttacaat aatcaagtgt tgatcttgat aaacttcctg
     3361 cactaagtct gggttgcccc aagggatcgt cttttgggga taatcttcct cgtcaaaagt
     3421 caactggcaa acatctccag gatttacgat ttcgttccag tggacttctt cttgatttat
     3481 caaaatatgt ttcttgattc tcaaaaaatg acggattttt ctagggatga ggagttgttc
     3541 ctcaagtaat tgctttaccg tcatttgagg tagagaggcg ggtaatgtaa atgtgaattg
     3601 catacagata ttgtaacaaa aaaagcccta tttggatagg aaatagctaa attcttgtct
     3661 tcctatgatg aagatgataa aataaacgca tgaaattaga taaattattt gagaaatttc
     3721 tttctctttt taaaaaagaa acaagtgaac tagaggactc tgattctact atcttacgtc
     3781 gctctcgtag tgatcgaaaa aaattagccc aagtaggtcc gattcgaaaa ttctggcgtc
     3841 gttatcatct aacaaagatt atccttatac taggtttgag tgcaggcttg ctagttggaa
     3901 tctatttgtt tgctgtagcc aagtcgacca atgtcaatga tttgcaaaat gccttgaaaa
     3961 ctcggactct tatttttgat cgtgaagaaa aagaggctgg tgccttgtct ggtcaaaagg
     4021 gaacctatgt tgagctgact gatatcagta aaaacttgca gaatgctgtt attgcgacag
     4081 aagaccgttc tttctataaa aatgacggga ttaactatgg ccgtttcttc ttggctattg
     4141 tcactgctgg acgttcaggt ggtggctcta ccattaccca acagctggct aaaaacgcct
     4201 atttatcaca agatcaaact gttgagagaa aagcgaaaga atttttcctt gccttagaat
     4261 taagcaaaaa atatagtaag gagcaaattc taaccatgta ccttaacaac gcttattttg
     4321 gaaatggtgt gtggggtgta gaagatgcga gtaagaaata ctttggagtt tctgcatcag
     4381 aagtgagtct ggatcaagct gcgactctgg cagggatgct caaggggccg gaactgtata
     4441 atcccttgaa ttccgtagaa gattctacta atcggcgcga tactgtcttg cagaatatgg
     4501 ttgcagcagg atatattgat aaaaaccaag aaaccgaagc tgctgaagtt gatatgactt
     4561 cgcaattgca cgataagtat gaaggaaaaa tctcagatta ccgttacccc tcttattttg
     4621 atgcggtggt taatgaagct gtttccaagt ataatctaac agaggaagag attgtcaata
     4681 atggctaccg catttacaca gagctggacc aaaactacca agcaaatatg cagattgttt
     4741 atgaaaacac atcgctattt ccgagggcag aggatggaac gtctgctcaa tcaggaagtg
     4801 tagctctcga accgaaaaca gggggagttc gtggagttgt cggtcaagtt gctgacaatg
     4861 ataaaactgg attccggaat ttcaactatg caacccaatc aaagcgtagt cctggttcta
     4921 caattaagcc tttagttgtt tatacgccag cagttgaagc aggctgggct ttgaataagc
     4981 agttggataa ccataccatg cagtatgaca gctatacggt tgataactat gcagggatca
     5041 aaacgagtcg agaagttcct atgtatcaag ccttggcaga atcgcttaat ctacctgctg
     5101 ttgccactgt taatgatttg ggtgtcgaca aggcttttga ggcaggcgaa aaattcggac
     5161 tcaacatgga aaaggtcgac cgtgttcttg gtgtcgcctt gggaagcggt gttgaaacca
     5221 accctcttca aatggctcaa gcatacgctg cctttgcaaa tgaaggttta atgcctgaag
     5281 ctcattttat tagtagaatt gaaaatgcta gtggacaagt tattgcgaat cataaaaatt
     5341 cacaaaaacg ggtgattgat aagtctgtag ctgacaagat gaccagtatg atgttgggga
     5401 ctttcaccaa cggtaccggt attagttcat cgcctgcaga ctatgtcatg gcagggaaaa
     5461 ctggaacaac tgaagcagtt ttcaatccgg agtacacaag tgaccagtgg gtaattggtt
     5521 atactccgga tgtagtgatt agccactggc ttggctttcc gaccactgat gaaaatcact
     5581 atctagctgg ctctacttca aacggtgcag ctcatgtctt tagaaacatt gccaatacca
     5641 ttttacctta tacgccagga agtaccttta cggttgaaaa tgcttataag caaaatggaa
     5701 ttgcaccagc caatacaaaa agacaagtac aaaccaatga taatagccag acagatgata
     5761 atttgtctga tattcgaggg cgtgcgcaaa gtctagtaga tgaggctagc cgggctatct
     5821 cagatgcgaa gattaaggaa aaggctcaaa caatatggga ttcgatagtc aatctatttc
     5881 gctaagatgc ttgtcaaagc ctagctttct tgttataatg gataagatgg aggcgttatg
     5941 gcactaaaaa aagcaagcct agcttgtgcg gtttgtggtt cgagaaacta ttcaatcaag
     6001 atcagcggaa accccaagcc tacacgacta gaagtaaata aattttgtaa gcattgtggc
     6061 aagtacacta cacacagaga aacgagatag gagagagcga tgcgttttat tggagatatt
     6121 tttagacttc ttaaagacac aacatggcca actcgcaagg aaagctggag agattttcgt
     6181 tctatcatgg aatacacagc tttctttgta gtaattattt acatttttga ccagttgatt
     6241 gtttcaggtt tgattcgatt tattaacatt ttttagaaga ttagtggagt taattacact
     6301 agaaatcttc tatttatgaa aggaaatatc atggatagtt ttgataaagg atggtttgtt
     6361 ttacaaactt attctggtta tgaaaataag gtgaaagaaa atctattaca acgtgcacaa
     6421 acctacaata tgttggataa tattctacgc gttgaaattc caacacaaac agtgcaagtt
     6481 gaaaaaaatg gaaagagaaa agaagtagaa gaaaatcgct ttccaggtta tgttcttgta
     6541 gaaatggtca tgacagatga agcttggttt gttgttcgaa acacaccaaa tgttacagga
     6601 tttgtcggat cacacgggaa cagatcaaaa ccaactccat tattggaaca agaaattcgt
     6661 gacattttgg tatctatggg acaaactgtt caagaatttg atttcgatgt tgagattggt
     6721 caaactgtac gtattattga tggtgctttt gcagactaca ctggtaagat tacagaaatt
     6781 gataataaca aagtgaaaat gattatctct atgtttggta atgacacagt tgcagaagta
     6841 aacctaaacc aaatcgcaga attataatcc tgagagaggc tggagcctct cttttttgtg
     6901 cagtttagga gctaaaggga acagaatgga gaaaatggaa caaatgtgtt ttctaatctg
     6961 ttagactgta tctagaaagg ggaaaattat gattaaagaa ttgtatgaag aagtccaagg
     7021 gactgtgtat aagtgtagaa atgaatatta ccttcattta tgggaattat cggattggga
     7081 ccaagaaggc atgctctgct tacatgaatt gattagtaga gaagaaggac tggtagacga
     7141 tattccacgt ttaaggaaat atttcaaaac caagtttcga aatcgaattt tagactatat
     7201 ccgtaagcag gaaagtcaga agcgtaaata cgataaagaa ccctatgaag a
//
LOCUS       DBEROU010000068         6838 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.068, whole genome shotgun sequence.
ACCESSION   DBEROU010000068 DBEROU010000000
VERSION     DBEROU010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.068"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(1..339)
                     /locus_tag="ACL8CL_001999"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACL8CL_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844241.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            537..2303
                     /gene="patB"
                     /locus_tag="ACL8CL_002000"
     CDS             537..2303
                     /gene="patB"
                     /locus_tag="ACL8CL_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HGS1331176.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMIDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            2442..3131
                     /locus_tag="ACL8CL_002001"
     CDS             2442..3131
                     /locus_tag="ACL8CL_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HGS1331177.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            3135..3314
                     /locus_tag="ACL8CL_002002"
     CDS             3135..3314
                     /locus_tag="ACL8CL_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331178.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNYDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            3301..4650
                     /locus_tag="ACL8CL_002003"
     CDS             3301..4650
                     /locus_tag="ACL8CL_002003"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HGS1331179.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            4774..6234
                     /gene="gltX"
                     /locus_tag="ACL8CL_002004"
     CDS             4774..6234
                     /gene="gltX"
                     /locus_tag="ACL8CL_002004"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HGS1331180.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGEHSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWIIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDKFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            6248..6682
                     /locus_tag="ACL8CL_002005"
     CDS             6248..6682
                     /locus_tag="ACL8CL_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HGS1331181.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAEDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT         2154 a         1307 c         1474 g         1903 t
ORIGIN      
        1 ttctaactta gtcttaagat aggccttaaa agcaggtgaa aagcgagggc atgctttggc
       61 agcttgtatg agtgcctacc gcagatgagg ggaaccccgt ttgatcattc ttccagctaa
      121 atcaatctga tctgactgat aaatagaaga atccagtcca gcgaaagctt gtaattgagc
      181 aagattatca aaggcatgaa tatttcgaat ctcggctaaa atgaccgccc ctaaacgatt
      241 cccaatccca gtaaccgtcg tgatgatcga gttgaactca gccatcaagt cattgacaca
      301 tgtttccgcc ttgtcaatga gcctcttgta atgtttgatg ttttcattac acgagataaa
      361 acgtttatgc gttatcaaac tcatgaccaa ttgaaacaaa aagctgtggt tagatccttt
      421 cggaaattgt caagcgattg gaggaaatga actaatccac agcggcttat tccaagtata
      481 ccacttgggc tttggcagta gctaactgcg ctaaatataa tataaggagg agtaaaatga
      541 agacagttca atttttttgg cattatttta aggtctacaa gttctcattt gtagttgtca
      601 tcctgatgat tgttctggcg acttttgccc aagccctctt tccagtcttt tctggacaag
      661 cggtgacgca gctagccaat ttagttcaag cttatcaaaa tggcaatcca gaacttgtat
      721 ggcaaagcct atcaggaatc atggtcaatc ttggcctgct ggttttggtt ctatttatct
      781 ctagtgtaat atacatgtgt ctcatgacgc gcgtgattgc agaatcgacc aacgagatgc
      841 gcaaaggcct ctttggtaag cttgctcagt tgacggtttc tttctttgac cgtcgacaag
      901 atggcgatat cctgtctcat tttaccagtg atttggataa tatcctccaa gcctttaacg
      961 aaagcttgat tcaggtcatg agcaatattg ttttatacat tggtctgatt cttgtcatgt
     1021 tttcgagaaa tgtgacgctg gctctcatca ccattgccag caccccattg gctttcctta
     1081 tgctgatttt catcgtgaaa atggcacgca aatacaccaa cctccagcag aaagaggtag
     1141 ggaagctcaa cgcctatatg gatgagagca tctcaggcca aaaagccgtg attgtgcaag
     1201 gaattcaaga ggatatgatg gcaggatttc ttgaacaaaa tgagcgcgtg cgcaaggcaa
     1261 cctttaaagg aagaatgttc tcaggaattc ttttccctgt catgaatggg atgagcctga
     1321 ttaatacagc catcgtcatc tttgctggtt cggctgtact tttgaatgat aagtctattg
     1381 aaacaagtac agccctaggt ttgattgtta tgtttgcaca attttcacag cagtactacc
     1441 agcctattat ccaagttgca gcgagttggg gaagccttca gttggccttt actggagctg
     1501 aacgaattca ggaaatgttt gatgcagagg aggaaatccg acctgaaaag gctccaacct
     1561 tcactaagtt gcaagaaagt gttgaaatca gtcatatcga tttttcatac ttgcctgata
     1621 aacctatttt gaaagatgtc agcatttctg cccctaaagg ccagatgaca gcagttgttg
     1681 ggccgacagg ttcaggaaaa acgactatta tgaacctcat caatcgcttt tatgatgttg
     1741 atgctggtgg tatttatttt gatggtaaag acattcgtgg ctatgactta gatagtctta
     1801 gaagcaaggt gggaattgta ttgcaagatt cggtcttgtt tagcggaacg attagagaca
     1861 atatccgatt tggtgtgcca gatgctagtc aggaaatggt tgaggtagca gcaaaagcaa
     1921 cccacattca cgactatatc gaaagtttgc ctgataagta cgatactctt attgatgatg
     1981 accagagcat cttttcaaca gggcagaagc aattgatttc aatcgctcga accctgatga
     2041 tagatccaga agttctcatt ctcgatgaag caacttcaaa cgtagatacg gtgacagaaa
     2101 gcaagattca gcatgccatg gaggtggttg tagcaggtag aactagtttc gtcattgccc
     2161 accgcttgaa aaccattctc aatgcagatc agattattgt ccttaaagat ggagaagtca
     2221 ttgaacgtgg taaccaccat gaacttttga agctaggtgg cttttattca gaactctatc
     2281 acaatcaatt tgttttcgaa taagaaagaa gttgtcctat gtgggcagct ttttcttgtc
     2341 cataaaaaat gtttatcaca gccttaaaaa aaacatatta gacgaaagtc attttgagtg
     2401 atatgatagg actatcgtta gcattcgaaa ggagaggcat catggctaga acggttgtag
     2461 gagttgctgc aaatctatgt cccgtagacg cagaaggcaa aatcattcat tcatctgtat
     2521 cttgtagatt cgcagagatc attcgtcaag tcggtggtct ccctttagtc attcctgttg
     2581 gtgatgagtc agttgtacgt gattatgtgg aaatgattga caaactcatt ttgacaggag
     2641 gccaaaatgt tcatcctcag ttttatggag agaaaaagac cgtcgagagc gatgattaca
     2701 atctggtccg tgacgaattt gaattggcac tcttgaagga agcgcttcgt cagaataaac
     2761 caattatggc aatctgtcgc ggtgtccaac ttgtcaatgt tgcctttggt ggaaccctca
     2821 atcaagaaat cgaaggtcac tggcaaggac tacctttcgg gacatctcac tctattgaga
     2881 cagtagaagg aagcgtggtg gctaagctat ttgggaaaga aagtcaggtc aactcagtcc
     2941 atcgtcaaag cattaaagat ttggcaccta atttccgtgt aactgctatt gattcaagag
     3001 accaaactat tgaagcgatt gagtctatcg acgagcaccg cattatcggt ttacagtggc
     3061 atccagagtt tctggttaat gaagaagatg gcaatttaga attatttgag tatttattga
     3121 atgaactgta acgattggaa catctggtcg ttctttcttt tattttttca aaatttttag
     3181 aatgtggcat tttacgcaaa cgtttgaatt atgataaaaa ttggagaaat tcgacaaaaa
     3241 aacttgaaaa aaacgaaggt aagcgttatg atagaaaaga agaaatattg gaggaagaac
     3301 atgtcacata ttaaatttga ttattcaaaa gttttagaca aatttgttgc accacatgaa
     3361 gtggaataca tgcaatcaca agtaacagca gcagatgaat tgatccgtaa aggaactggt
     3421 gctggtagcg acttcttggg atggttggac cttcctgaaa aatacgatcg cgaagaattt
     3481 gaccgcatct tgaaagctgc tgagcaaatc aagtcagaca gcgatgtttt ggttgtgatc
     3541 ggtattggtg gatcttacct tggtgcaaaa gcagcaatcg acttcttgaa tcaccatttt
     3601 gctaacttgc aaacaaaaga agaacgcaaa gctccacaaa tcctttacgc aggaaactca
     3661 atctcatcta cttaccttgc tgacttggta gagtatgttg cagacaaaga cttctcagta
     3721 aacgtaattt ctaaatcagg tacaacaact gaaccagcga ttgctttccg tgtctttaaa
     3781 gaactcttgg ttaagaaata cggtcaagaa gaagctaaca aacgtatcta tgcaacaact
     3841 gaccgccaaa agggtgctgt taaggttgaa gcagacgcta acggttgggg aacatttgtt
     3901 gttccagatg atatcggtgg acgcttctca gtattgacag ccgttggttt gcttccaatc
     3961 gcagcatcag gagctgacat caaagctctt atggaaggtg cgaatgcagc tcgcaaagac
     4021 tacacttcag acaaaatctc tgaaaacgaa gcttaccaat acgcagctgt tcgtaacatc
     4081 ctttatcgta aaggctatgc aactgagatc ttggtaaact atgagccatc acttcaatac
     4141 ttctcagaat ggtggaaaca attggctggt gaatcagaag gaaaagacca aaaaggtatc
     4201 tacccaactt cagccaactt ctcaactgac ttgcactcac ttggtcaatt tatccaagaa
     4261 ggaactcgta tcatgtttga aacagttgtc cgtgttgaca aacctcgtaa aaacgtgctt
     4321 attcctactt tggaagaaga ccttgacgga cttggttacc ttcaaggaaa agacgttgac
     4381 tttgtaaaca aaaaagcaac tgacggtgtt cttcttgccc acacagatgg tgatgtacca
     4441 aacatgtatg tgactcttcc agagcaagac gctttcactc ttggttacac tatctacttc
     4501 ttcgaattgg caattgccct ttcaggttac ttgaatgcta tcaacccatt tgaccaacca
     4561 ggtgttgaag cttataaacg taacatgttt gcccttcttg gaaaaccagg atttgaagaa
     4621 ttgagcaaag aacttaacgc acgtctataa tagaagaaaa gagtggtttg cccactcttt
     4681 ttactctctt tatccataga aattggactc agccaagact tgtgatataa tatagaaagc
     4741 aaaaaggcag acgcctagat aataggagaa actatgtcaa aagatatccg cgtacgttac
     4801 gcaccaagtc caacaggact actacacatc ggaaatgctc gtacagcatt gtttaattac
     4861 ttgtatgcgc gccatcatgg tggaacattt ctcatccgta tcgaagatac tgaccgtaaa
     4921 cgccatgtcg aggatggtga acattcacaa cttgaaaacc ttcgctggtt aggcatggat
     4981 tgggatgaaa gtccagaatc acatgagaat tatcgccagt ctgagcgttt ggacttgtat
     5041 caaaaataca ttgaccaact attagctgaa ggaaaagcct ataaatctta cgttacagaa
     5101 gaagagttgg cagctgaacg cgaacgccaa gaagtagctg gcgaaacacc acgctacatc
     5161 aatgaatacc ttggtatgag tgaagaagaa aaagcagctt acatcgcaga acgtgaagca
     5221 gcagggatca tcccaactgt tcgtttggct gtcaatgagt caggtatcta caagtggcat
     5281 gatatggtca aaggcgatat cgaatttgaa ggtggcaata tcggtggtga ctggattatc
     5341 caaaagaaag acggttaccc aacttacaac tttgccgttg ttatcgatga ccacgatatg
     5401 caaatctctc atgttatccg tggagatgac catattgcta atacaccaaa acagcttatg
     5461 gtctatgaag ctcttggttg ggaagctcca gagttcggtc acatgacctt gattatcaac
     5521 tctgagactg gtaagaagtt atctaaacgt gatactaata cacttcaatt tatcgaagat
     5581 taccgaaaga aaggttacct tccagaagca gtctttaatt ttattgctct tcttggttgg
     5641 aacccaggtg gtgaagatga gattttctct cgtgaagaac tcattaaact ttttgatgaa
     5701 aaccgcctca gcaagtcacc agcagccttt gatcagaaaa aactagactg gatgagcaat
     5761 gattatatca agaatgcaga ccttgaaacc atctttgaaa tggcaaaacc attcttagag
     5821 gaagcaggcc gtttgactga caaggctgaa aaattagttg agctctataa accacaaatg
     5881 aaatcagtag atgagattat cccattgaca gatcttttct tctcagattt cccagaattg
     5941 acagaagcag agcgcgaagt catgacgggt gaaacagttc caacagttct tgaagcattc
     6001 aaagcaaaac ttgaagcgat gacagatgat aaatttgtga cagaaaatat cttcccacaa
     6061 attaaagcag ttcaaaaaga aacaggtatt aaagggaaaa atcttttcat gcctattcgt
     6121 atcgcagttt caggcgaaat gcatgggcca gaattaccag atacaatttt cttgcttgga
     6181 cgtgaaaaat caattcagca tatcgaaaac atgctaaaag aaatctctaa ataaagagga
     6241 tacaataatg gacatctggg aaaagatgta cgaagaagca cagaaactat acaatccaca
     6301 tgaagtatca gactttgttt atgccaatca tgttgtagct gcagtagaag cagaagatgg
     6361 acaaatattt acaggattct gtatggaggg aacctgtggt gttttccatc tctgcgcaga
     6421 acgggcggcc ctcttcaata tgtaccaatt ttcaggacaa actaaggtta agaaagtatt
     6481 agcctttcga gacaaaccac cttatggtgg aagttcagcc atgccttgcg gtgcttgtag
     6541 agaattcctt ttagagctta atgctgaaaa taaagatgca gaattcatga tggactataa
     6601 tataagaaaa acagttaaag tcgcagaact aatcccttat tggtggggag aagaacgtgc
     6661 ttctaagttt aatgagcgat agaagagtca gtataattct ggcttttttt actataagtt
     6721 gaaaaaataa aaaaaaatta gaaaaagtag ttgacaaagt ttgaaaagac tgtataatag
     6781 taagagttga aaataacaac tcaggtccgt tggtcaaggg gttaagacac cgcctttt
//
LOCUS       DBEROU010000069         6120 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.069, whole genome shotgun sequence.
ACCESSION   DBEROU010000069 DBEROU010000000
VERSION     DBEROU010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.069"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            1..129
                     /locus_tag="ACL8CL_002006"
                     /pseudo
     CDS             1..129
                     /locus_tag="ACL8CL_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            282..482
                     /locus_tag="ACL8CL_002007"
     CDS             282..482
                     /locus_tag="ACL8CL_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331182.1"
                     /translation="MVVSASASSTSTQAQEQVDKSELRALSQELDQRLKALATVSDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            507..924
                     /locus_tag="ACL8CL_002008"
                     /pseudo
     CDS             507..924
                     /locus_tag="ACL8CL_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642281.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane domain-containing protein"
     gene            1155..1862
                     /gene="ccdA2"
                     /locus_tag="ACL8CL_002009"
     CDS             1155..1862
                     /gene="ccdA2"
                     /locus_tag="ACL8CL_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HGS1331183.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQERAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYVMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1873..2430
                     /gene="sdbB"
                     /locus_tag="ACL8CL_002010"
     CDS             1873..2430
                     /gene="sdbB"
                     /locus_tag="ACL8CL_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HGS1331184.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIS
                     VGKDVPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2804..4195
                     /locus_tag="ACL8CL_002011"
     CDS             2804..4195
                     /locus_tag="ACL8CL_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HGS1331185.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
     gene            4338..5255
                     /gene="adcAII"
                     /locus_tag="ACL8CL_002012"
     CDS             4338..5255
                     /gene="adcAII"
                     /locus_tag="ACL8CL_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HGS1331186.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLESDPQNDKTYLENLEENMSILAEELK"
     gene            5263..>6120
                     /gene="phtB"
                     /locus_tag="ACL8CL_002013"
     CDS             5263..>6120
                     /gene="phtB"
                     /locus_tag="ACL8CL_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HGS1331187.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKNEL
                     SASELAAAEAYWNGKQGSRPSSSSSYNANTAQPRLSENHNLTVTPTYHQNQGENISSL
                     LRELYAKPLS"
BASE COUNT         1872 a         1102 c         1291 g         1855 t
ORIGIN      
        1 gcaggtctaa caaatggtga gttaatcgtt ccaatgactt acgaagagac gatgacgagc
       61 gacttttttg aagcttggtt tcagaagttt ttcttaccaa cattaaacac accatcggtt
      121 attatagtaa aatgaaataa gaacaggaca atcaaatcaa tttctagcaa tgttttagaa
      181 gtccatatgt actattctag tttcaatata ccataactgc tcctagtcaa cctgcaagtc
      241 ctgtcccagc tccaagctca gctccgactc aagatcctag agtggtagta agcgcttcgg
      301 ctagctcaac gagcacacaa gctcaagagc aagttgacaa gtctgaactt cgtgccttga
      361 gtcaagagtt agaccaacgc ttgaaagctt tggcaacagt atctgatccg aaaatcgatg
      421 caaccaaggc tgtcctccta gatgctcaaa aagctccgga agatagtgcc ttgacagagt
      481 aaggtctgca tactgcagta gagtctgtga agactgcctt ggactctctc aaagatgtga
      541 tggcaaatgc tagcgacagc aagcctacac aggacaagaa ggatgcaaaa caaggaacgg
      601 aagatagtaa ggattcagat aagatgactg aaacaaactc agttccagca ggagtgattg
      661 tggtcagtct acttgccctc ctaggcgtga ttgccttctg gctaattcgc cgtaagaaag
      721 agtcagaaat ccagcaatta agcacggaat tgaccaaggt tctaggacag ctagatgcag
      781 aaaaaagcgg ataaaaaagt ccttgccaaa gcccaaaacc ttctccaaga aacccttgat
      841 ttcgtgaaat aagaaaatgg ctcagcagag acagaaacta aactagtaga ggagcttaaa
      901 gcaatccttg acaaactcaa gtaaaacatg atttatgaaa tgtcagacct aaaagacatt
      961 ttcgttcaca aaagcagcga cgagtaatcc ttgtcgttgc tttttggcta ttttggggga
     1021 taagatccgc cttgactagc aaaagaaaag attggtccaa tagtgcccga tacgattttg
     1081 cgtagcagaa agcttacaaa taaaattgaa tatagtaaaa taggattaga attattaaga
     1141 aagttggttc tttattggaa acgatagtat ttttaatctc tgtttttcta gcaggtgttt
     1201 tatccttttt ttctccttgt atttttcctc ttctgccagt ctatgctggg attttattgg
     1261 atgatcaaga aagggcaaaa agcttttctt tgtttgggag aaaggttctc tggtcaggct
     1321 tgattcgaac actttgcttt atcgctggta tctctctcat tttctttatt ctaggctttg
     1381 gtgctggtta ctttggtcat attctctatg caaattggtt tcgatatgtc atgggagcta
     1441 ttattatcat tttgggtctt caccagatgg aaatttttca tttgaagaaa ttagaagttc
     1501 aaaaaagttt tacctttaaa aaatcagatt ctaatcgtta ttggtcagct tttttacttg
     1561 gtattacctt tagctttggt tggacacctt gtattggtcc agttttaagt tctgttttag
     1621 cacttgcggc ttctggaggc aatggtgctt ggcaaggagc gatttatact ctcatttaca
     1681 ctctgggcat ggcccttcct ttcttggtct tggcactagc ttcaggtcta gtcatgccat
     1741 attttagtaa aatcaagcgt catatgatgc tactaaagaa aattggtggt ttcctcattg
     1801 ttttaatggg aattttgtta ctattaggac aagtaaatgt tctagctgga atttttgaat
     1861 aaaggaggac aaatgaaaaa agtaatgttt gctggcttaa gtctcttgtc attagttgta
     1921 ttgatggcct gtggtgagga agaaactaaa aagactcaag cagcacaaca gccaaaacaa
     1981 caaacgactg tacaacaaat ttctgttgga aaagatgttc cagactttac attgcaatcc
     2041 atggatggca aagaagttaa gttatctgat tttaagggta aaaaggttta cttgaagttt
     2101 tgggcttcat ggtgtggtcc atgcaagaaa agtatgccag agttgatgga actagcggcg
     2161 aaaccagatc gtgatttcga aattcttact gtcattgcac caggaattca aggtgaaaaa
     2221 actgttgagc aattcccaca atggttccaa gaacaaggat ataaggatat cccagttctt
     2281 tatgatacca aagcaaccac cttccaagct tatcaaattc gaagcattcc tacagaatat
     2341 ttaattgata gccaaggaaa gattggaaag attcaatttg gtgctatcag taatgcggat
     2401 gcagaagcag catttaaaga aatgaactag aatcgataaa atctgattac aagatgtagt
     2461 cagattttgg taacttctaa aactaacttc cagtttccca caacctagct tttagtatga
     2521 gaaaaacgcg tgacatcagt ggaaatccgt cttagactaa cttccactgg ttttcttttt
     2581 cttgatatat aagggttcaa aagcgaaaag actcagaaaa agtgcacgac aaatagccct
     2641 aaaacagagt cgtcttagag taacttccag ttttgggaga gagatggaag ttactttgag
     2701 aagttacggt agtcagattt ttttagaaaa acattttata aatattcaca tatctcctat
     2761 atttatggta aaatagaatt atcagtttat tttggagtca aagatgaata tatttagaac
     2821 aaagaatgtt agtttagata aaacagagat gcataggcat ttgaagttat gggatttgat
     2881 tttgctgggt atcggagcca tggtagggac aggcgtcttt acaatcacag gtactgcagc
     2941 tgcaacactt gctggcccag ccctagtgat ttcaatcgtt atttctgcct tgtgtgtggg
     3001 attatcagcc ctcttttttg cagaatttgc ctcgcgagta cccgctacag gaggtgccta
     3061 tagttacctc tatgctatct taggagaatt ccctgcctgg ttggctggtt ggttaaccat
     3121 gatggagttc atgacagcca tatcaggcgt agcttcgggt tgggcagctt attttaaagg
     3181 cttgctctct caatacggga tagcctttcc tcaggcttta aatggtacct ttaatcctca
     3241 agcagggaca tttgttgatt tattgcctat tcttgtcttg gtcttggtga cttcgcttgt
     3301 tttactaaat gccaaggcag ccttacgctt taattcgatt ctagttattt tgaaattttc
     3361 cgctttagct ctctttgttt tagtaggaat ttggaatatt aagtttgata attggagcaa
     3421 ttttgctcct tacggttttg gacaaatcta tggagctagt actggtatta tggctggtgc
     3481 atccttgatg ttcttcggtt ttttgggatt tgaatccatc tctatggctg tagatgaagt
     3541 caagactcct caaaaaaata ttcctagagg gattgtctta tcgctttcta tcgtaaccat
     3601 tctctatgcc ttggtgaccc ttgttttgac tggtgtggtt cactatagtc atttaaatgt
     3661 cgatgatgcc gttgcctttg cccttcgtag tgttgggatt agttgggcag ccaactatgt
     3721 gtcattagtg gctatcttga ccttgattac agtttgtatt tcgatgacct atgccctatc
     3781 gcgtatgatt tacagtttag cacgtgacgg cttagtgcct gctgccttta aggaactgac
     3841 gaagactagc aagataccaa agaatgctac tatcttgaca ggtctagctt cagcagtagc
     3901 agcaggaatg ttcccactag ccagtattgc agccttttta aatatttgta ccttagccta
     3961 cttgatcatg ttggcttatg gtctgattcg cttacggaaa gaaaaaggaa tgcccaaagc
     4021 tggggaattt aaaacaccat tggtacctct gttaccaatt ttatcgatca tcatttgttt
     4081 atccttcatg ttacagtata atatgaatac ctggattgct ttcctagttg cgttgttagt
     4141 aggaagtatt atttacttca cttatggcta taagcattct accatagaag aataaagtga
     4201 aagtctattg agttgttgac actcagcaga tttttatatg tgaaagaaat ttcaaatttt
     4261 ttctaaaaat atcttgacag attaaatttt caggagtaga atatttacta gttaattaaa
     4321 tggttaagga gttgttcatg aagaaacaaa atttattttt agtcctgtta agtgtctttc
     4381 ttttgtgctt gggggcttgt ggtcaaaagg aaagtcagac aggaaagggg atgaaaattg
     4441 tgaccagttt ttatcctatc tacgctatgg ttaaggaagt atctggtgac ttgaatgatg
     4501 ttcggatgat tcagtcaagt agtggtattc actcctttga accttcggca aatgatatcg
     4561 cagccatcta tgatgcagat gtctttgttt accattctca tacactcgaa tcttgggcag
     4621 gaagtctgga tccaaatcta aaaaaatcca aagtgaaggt cttagaggct tctgagggaa
     4681 tgaccttgga acgtgtccct ggactagagg atgtggaagc aggggatgga gttgatgaaa
     4741 aaacgctcta tgaccctcac acatggctag atcctgaaaa agctggagaa gaagcccaaa
     4801 ttatcgctga taaactttca gaggtggata gtgagcataa agagacttat caaaaaaatg
     4861 cgcaagcctt tatcaaaaaa gctcaggaat tgactaagaa attccaacca aaatttgaaa
     4921 aagcgactca gaaaacattt gtaacacaac atacagcctt ttcttatcta gcgaagagat
     4981 ttgggcttaa tcaacttggt attgcaggta tctctcctga acaagaacca agtccacgac
     5041 aactaacaga aattcaggaa tttgttaaga cctataaggt taaaacgatt tttacagaaa
     5101 gtaacgcttc ttcaaaagta gctgaaactc ttgtcaaatc aacaggtgtg ggtcttaaaa
     5161 ctctgaatcc tttagagtca gacccacaaa atgacaagac ctatttagaa aatcttgaag
     5221 aaaatatgag tattctagca gaagaattaa agtgaggaaa gaatgaaaat caataaaaaa
     5281 tatctagcag gttcagtggc agtccttgcc ctaagtgttt gttcctatga acttggtcgt
     5341 caccaagctg gtcaggttaa gaaagagtct aatcgagttt cttatataga tggtgatcag
     5401 gctggtcaaa aggcagaaaa tttgacacca gatgaagtca gtaagaggga ggggatcaac
     5461 gccgaacaaa tcgtcatcaa gattacggat caaggttatg tgacctctca tggagaccat
     5521 tatcattact ataatggcaa ggtcccttat gatgccatca tcagtgaaga gctcctcatg
     5581 aaagatccga attatcagtt gaaggattca gacattgtca atgaaatcaa gggtggttat
     5641 gtcattaagg taaacggtaa atactatgtt taccttaagg atgcagctca tgcggataat
     5701 attcggacaa aagaagagat taaacgtcag aagcaggaac gcagtcataa tcataactca
     5761 agagcagata atgctgttgc tgcagccaga gcccaaggac gttatacaac ggatgatggg
     5821 tatatcttca atgcatctga tatcattgag gacacgggtg atgcttatat cgttcctcac
     5881 ggcgaccatt accattacat tcctaagaat gagttatcag ctagcgagtt agctgctgca
     5941 gaagcctatt ggaatgggaa gcagggatct cgtccttctt caagttctag ttataatgca
     6001 aatacagctc aaccaagatt gtcagagaac cacaatctga ctgtcactcc aacttatcat
     6061 caaaatcaag gggaaaacat ttcaagcctt ttacgtgaat tgtatgctaa acccttatca
//
LOCUS       DBEROU010000070         5979 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.070, whole genome shotgun sequence.
ACCESSION   DBEROU010000070 DBEROU010000000
VERSION     DBEROU010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5979
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.070"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(170..1396)
                     /locus_tag="ACL8CL_002014"
     CDS             complement(170..1396)
                     /locus_tag="ACL8CL_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HGS1331188.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQILQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLLNLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTINYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPVSTLFLRNQPFLNLQTGQSLALLSTFEL
                     SALIVGSLVSGYLQHTISIKTALYASLVIQLLLLVGFATVRFDWILIFSTLDAFFAGV
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(1468..2133)
                     /locus_tag="ACL8CL_002015"
     CDS             complement(1468..2133)
                     /locus_tag="ACL8CL_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HGS1331189.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVAGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVAWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEDMIYRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            complement(2201..2398)
                     /locus_tag="ACL8CL_002016"
     CDS             complement(2201..2398)
                     /locus_tag="ACL8CL_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide pheromone VP1"
                     /protein_id="HGS1331190.1"
                     /translation="MLNLQFAETMELTEAELETVYGGEFGNNAVIPAGAWGGLGTSWS
                     ITNFWKKYFNHDSSTVNRRHY"
     gene            complement(2689..3552)
                     /locus_tag="ACL8CL_002017"
     CDS             complement(2689..3552)
                     /locus_tag="ACL8CL_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HGS1331191.1"
                     /translation="MIERMELGEFYKELRLARKLKQTDVACEGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMRIGRKVVNRFAHQDIAALEQLLEEV
                     DQEQMAQTYRRLNAIVIKDAIHSLNKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKQGLLNILSELMERKLFSYIPIFE
                     AELERMLRPYDVFEKVSWQFLKKMSVFLQTKGSNQKEIERFIQSLQVLENPQLTSLFE
                     LRFQQYKELID"
     gene            complement(3947..5118)
                     /locus_tag="ACL8CL_002018"
                     /pseudo
     CDS             complement(3947..5118)
                     /locus_tag="ACL8CL_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
     gene            complement(5131..5880)
                     /locus_tag="ACL8CL_002019"
     CDS             complement(5131..5880)
                     /locus_tag="ACL8CL_002019"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HGS1331192.1"
                     /translation="MSNNTKYIFLSPHLDDAIFSCGDYISKLTSEGEIVLVITIFSGY
                     PLSQQLQPSAKQFHKLCNLGKYPIEERKKEDRLACERLQCDFRHLSYYECLYRKDRNG
                     NFLYRHIYSELKNEDTLKNDIIKELLMHLDDKCVVYCPLSLGDHVDHVFVNSIGRALE
                     FMRYKVIYYEDFPYVSDSSMVSYMGKTKELKMYQEELDEKHYIDRISSILCYKSQILI
                     IWKSVEKLLNNIKELYLRNGAAYSIIFWIKK"
BASE COUNT         2075 a         1125 c          924 g         1855 t
ORIGIN      
        1 cgattgggaa agtaaaatta atttctataa atgttttagc aattgtttcg tactatttta
       61 gattcagtct attataacac attcagaaaa gagaaaaaag tctgttgatt ttgaccatca
      121 taaaaagact ggcaatccag tctcaaacat atattataga aattctccac taaatacttt
      181 cacgaatatt cagaagcata acaaaggcaa ctagaagaaa tagcaataaa acaaagctaa
      241 ctgccagagt tccaaagcta gtagcaatgg ttaccaaagc tattgtaaat aagctaggta
      301 aaacaaccgt aatggcaccg atagaggatt gaactgctcc cattgactcc tcaggtattt
      361 gtttaaaaac gagttcttgc aatctaggag agagaacacc tgcgaaaaag gcatccaagg
      421 tactaaagat gagaatccag tcaaaacgaa ctgtggcaaa tcctactaga agaagcaact
      481 ggatgacaag tgaggcatag agagctgttt ttatggaaat ggtatgttgc agatagccac
      541 ttacaaggct tccgacaatc agggctgaca gttcaaaagt ggatagcaag gcaagagatt
      601 gacctgtttg taaattcaaa aagggttgat tcctcaaaaa tagagtagag acaggaactg
      661 taacatttat cactgcttga ctagtagaga taataaacaa aaccaagagc accttattca
      721 tattccatat caatttcgat gattggagca aatgctggca aaaggatttt acagagagtc
      781 cttcttgata gttaatcgtt ttttctactt tcaagaggtc agtttttatg aagaggatac
      841 ctaaaaatgc gattaaaaag gtaagagcgt tcagcaagga aatgaactgg atggagagaa
      901 tccctagcaa gactcctcct agaatattac tgattgtttt cactaaacta acagttgact
      961 gtttaaatcc aatagcttct gccagatggt cttgcccaat aattctaatg aaaatcggag
     1021 tgagcatggc tcctgaaaag taactcaatg tgtcagacaa gaggttaagc agacaaataa
     1081 atgctactag caacaaggag aaagactgcc ctgaaagtga taaagaaacg atagagtaaa
     1141 gcaaaaattt tgcaaaacta atgactgtgt atttcaagac acgatgatgt tgaaaatctg
     1201 ccaaaactcc cagaaagatt tgtaaaattt ggggaagggt ttctgaaatc gtaatgagta
     1261 aaatcgccaa aggtgcaaaa gacgcatctg ccacataatt cagaaaggcc agataaaaaa
     1321 tcgtatcccc aagcgttgaa atccactggt tgatagttaa ttgcctaaaa tctctatttt
     1381 gaagaaatac tttcatcaca actccttctt aagttcaaat gggaatcttt ccccaaggat
     1441 agaccgcgat actactaaca accaaaatta cagtaacatc aaaagctgac caatgccatt
     1501 gtagactata tgcagtccaa taggccaata aattgacttt gtcattctaa ataagactgc
     1561 aaatataaga cctccaccca tatagaagac aaagtctgtc aagacccaac cgtgattact
     1621 aatgtgcgag accccaaata aaacagcgga accaagtaca tctagccccc atttctttcc
     1681 tttttccaga gcagtcatca ctaatccacg ataaatcatg tcttcaaaaa tgggacctgc
     1741 aatcacagga taaaaaaaat acatcaaaaa tgccgtagct cctgtaaaag taggagcagc
     1801 atgttgataa gaaatttcat ttcgagtagg tgggaaaaga aaaaaggtga caaaattcca
     1861 agcaacaaaa gcaagcagag ctaggaagga atagaaaaga taggatcctt taaactttct
     1921 actattgatt ttctgccatt tccccgacca aatcatagca ataagagcaa ataaaaccac
     1981 aagaaaattc aacatcatat ccgacagata ataggcaaag tcagatagcc cagcaacaag
     2041 gtcgctgcgt aaaactaaaa cactgaactt ctggtcagca ataactagta gaaaaactat
     2101 aataaagtag cggtgtgaga ttatcttttt cattctattt tctcctatac tatgaaataa
     2161 agacagtccc tacccaagaa aaaggcaagg actctgaata tcaataatgg cgacgattaa
     2221 cagtggaaga atcatggtta aaatatttct tccagaaatt agtgattgac caagacgttc
     2281 ctaaacctcc ccaagcaccg gctgggataa cagcattatt cccaaattcc cctccataaa
     2341 ctgtctccaa ctcagcttct gtcaattcca ttgtttctgc aaattgtaaa tttaacatct
     2401 tttacactcc ttcaattatc ttcatttgta aaccacttct gcgacctagg atttgcttca
     2461 agtgctttac aagtacagta taacacgaaa attggcttat tttaaaaaat cgcatatttg
     2521 atattttttc ttatagaaat ttcttatttg cgattttgat agatttgatt atttccctgg
     2581 tataataaag ttattactaa caaggaggaa tataatagat gaagaaaaga aaaatccaac
     2641 tgatactcct acttatttcg gagtgggtta ttgtcattcc atttttaact aatctataag
     2701 ttctttatat tgctgaaaac gcaattcaaa aagggatgtt aattgtggat tttctaatac
     2761 ctgcagagat tgaataaagc gttcaatctc tttttgattg cttccctttg tttgaagaaa
     2821 gacactcatc ttctttaaaa attgccacga tactttttca aaaacatcat acggtcgtaa
     2881 catcctctcc aactcggctt cgaagattgg gatgtaggag aaaagttttc gctccatgag
     2941 ttctgataag atatttaaga gtccttgctt catatacaat cgattgtgta ctaactcttt
     3001 aaattctttg gatttttcga gtaaggaggt tgataaaaaa atcagatctt gattgctcaa
     3061 gaagggcatg gtattgcaaa agagatagag ttcaaaccag gtccaagact cgatagcata
     3121 gagataggtg gtcaaaaact cgctatcctc ctctgctagt gggtagcttt tatttagtga
     3181 atggatggca tctttaatca cgatggcatt caaacgacga taggtctgcg ccatctgttc
     3241 ttgatcgact tcctccaata gctgctctaa agcagctata tcctgatggg caaagcgatt
     3301 cacaaccttt cgaccgattc gcatatgtgg agattcttga tagttgttga gcttgtgccc
     3361 aaactcatca aaggtcacat ttataccttg gatagctaga atcaacttat ccgcagacag
     3421 catagactgc cctagttcaa acttggacaa ctgagaagct gttagaccct cacaagccac
     3481 atctgtctgc ttaagctttc tcgccaaacg taattccttg taaaattccc ccagttccat
     3541 tctctcaatc atctgaccac ctcctagctt ttgcatatta tatcattatt tacaatcatt
     3601 tgtcaaaata ttctgatatt taagagggca aaaaagccag ccttaagctg actctaaaag
     3661 gaccgagcct tgtacaatac agaactcaat cctcgttcta aatagaatgt ctaacttttt
     3721 tgggcagtac acgagcttgg ggaacagcca ctggaacgat gaggtgtgag ctcaaaatat
     3781 cctccagtta tgtttttcct aatagtatac cggaagagtg aaaggatttt ataatggagc
     3841 ggttacaaag aacctacttt ctattaaaca gtatactatg aaaatgtgaa aatttaacat
     3901 ttttttgtac aaattttata aattattgcc tttttaatat caatagttaa tctcttatcc
     3961 agatccccct tgtgtaaact ttatctttat aagcttcaag gcccctatcc catctatttg
     4021 caacaattag atcactttgt tttgtaaata gttcaaaatt cttttcaata attacgttat
     4081 ctatactaac gtttaaattt ggttcatata ctaaaatttt tataccgaca atcaatagtt
     4141 cattaattat acttaaaata gctgactctt tgtaattatc tgaattatat ttcatcccca
     4201 atttatatat tcctactatc tttggctttc gttccaatat ttgtttaact atgaactgtt
     4261 ttctatttgt gtttgaaata tcaatcgctt ctatcactgg ggcatttatt tctataaatt
     4321 ctttttttaa ttgtttagta tctttgggaa gacaatatcc tccaaatcca aaagaaggat
     4381 tattataaaa atttccaatt cttggatcta aacaaacacc ttttattaca acttcagcat
     4441 ttaagcttct cctctcagca aaagaatcta gttcattaaa aaagcaacac ggagagctaa
     4501 gaatgtgtta gaaaaaagct taattgcttc tgcttcagta ggagaaacta acataacatt
     4561 tttaatattg gcagtactat gagtactaat cgaaaggaac aactctgcaa tttttcttcc
     4621 ttcaactgtc tcatctccaa caactatgcg acttggatat aaattatcat atatagaaca
     4681 accttctctc aaaaattcag ggacaaaaat gatatttttt gtatcaaaca gcctttttaa
     4741 tttgtttgaa aagccgatcg gaactgttga ctttaaaata atctttccat taggttttac
     4801 cctcagaatc ttcgataccg tttgttcgat ttcatatgta ttaaaactac caattttctc
     4861 atcataatct gtcggaagcg caataatata ataatcaata ttatttttaa tttcagaaaa
     4921 tgtatcaaaa aaagtaatat ttaagttatt ctcgcaaaaa aacttcataa gctcttcatt
     4981 tttagatgga agaatgccct tttttaaatt atttattttt acagaatcta tatcatatgc
     5041 aacaacttta tatttagatg caaatagtaa cgcgtaggcc agcccaacat gccccaaacc
     5101 aattactgct atattcataa aactacttcc ttatttctta atccaaaata taatagaata
     5161 agctgcccca ttccttaaat acaactcttt aatattgttt aaaagttttt caactgattt
     5221 ccagattatc aaaatctgag atttatagca caatattgat gatattctat caatataatg
     5281 tttttcatcg agttcctctt gatacatttt taattcttta gtttttccca tataactaac
     5341 catactacta tcacttacat atgggaaatc ctcataatat attactttat aacgcataaa
     5401 ttcaagcgcc cttccaatac tattcacaaa aacatgatca acatggtcac caagtgaaag
     5461 cggacaatat acgacacatt tgtcgtctaa atgcattaac agctctttta tgatatcatt
     5521 ctttaatgtg tcctcatttt ttaattcact atagatatga cggtatagaa aattgccatt
     5581 tctatctttc ctatagagac attcatagta cgataagtgt ctaaaatcac attgtagacg
     5641 ttcacaagct aacctatctt ctttcttcct ttcttcaatc ggatatttcc caaggttaca
     5701 caacttatga aattgcttag cagagggctg tagctgttga ctcaaagggt aaccagaaaa
     5761 tatagtaata acaagtacaa tttctccttc tgaagttaat tttgaaatat aatcaccaca
     5821 ggaaaaaatt gcgtcatcta aatgtggaga taaaaagata tacttagtat tgttactcat
     5881 aaccattccc tctacaattt atctaaaaac tcactaagtg tctgattaaa ttccacatca
     5941 tcaaaaaaat tcaccttatt cttaataatg aatatttcg
//
LOCUS       DBEROU010000071         5932 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.071, whole genome shotgun sequence.
ACCESSION   DBEROU010000071 DBEROU010000000
VERSION     DBEROU010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5932
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.071"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..1505)
                     /gene="glpK"
                     /locus_tag="ACL8CL_002020"
     CDS             complement(<1..1505)
                     /gene="glpK"
                     /locus_tag="ACL8CL_002020"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HGS1331193.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEID"
     gene            complement(1664..3091)
                     /locus_tag="ACL8CL_002021"
     CDS             complement(1664..3091)
                     /locus_tag="ACL8CL_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1331194.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELTIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     HQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLDLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPAFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QTPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3256..4128
                     /gene="hslO"
                     /locus_tag="ACL8CL_002022"
     CDS             3256..4128
                     /gene="hslO"
                     /locus_tag="ACL8CL_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HGS1331195.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGTGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            4115..5095
                     /gene="dusB"
                     /locus_tag="ACL8CL_002023"
     CDS             4115..5095
                     /gene="dusB"
                     /locus_tag="ACL8CL_002023"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HGS1331196.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            5191..5932
                     /locus_tag="ACL8CL_002024"
                     /pseudo
     CDS             5191..5932
                     /locus_tag="ACL8CL_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         1804 a         1352 c         1171 g         1605 t
ORIGIN      
        1 tcatcgattt ccgcaaagac ttgagttgct ttcacagcct tcttccagcc cttgtagagt
       61 tgttccttgc gagattcgtt catagatggc tcaaagagtt ctcctgtctc gttcaagagt
      121 ttcaactcgt ccaagtcttt ccagtaccct actgacaaac ctgctaggaa ggccgctcct
      181 agagctgttg tttccaggtt tttagcacgt gcaatgtcaa tgcctaaaat atccgcctgg
      241 aactgcatga ggaagttgtt catggctgca ccaccatcca ccttcagtac ttgaatggcg
      301 gtctgagtat ccacttgcat ggtgtcgatg atatcacgca cttgataagc aatagattgc
      361 aaagtcgcct tgataaagtc ttctttgctt gttccacgag tcaaaccaaa gacggaacca
      421 cgagcatttt ggttccagta tggagcgcct agacctgtaa aggctggaac gacataaact
      481 tcatcgttgt tgtgagaatc acgagcgtat ttttcagatt ctggtgaatt ttcaaccatg
      541 cgaagaccgt cacgaagcca ctgaatagca cttcctgcga tgaagataga accttccaag
      601 gcataataaa ccttaccgtt gattccgtaa ccaatggttg tcaagaggtt gttttcagac
      661 aactgcatct cttccccagt attcatgatg atgaaagagc ctgttccata agtattctta
      721 accatacctg gctcaaaagc caactgtcca aagagggctg cttgttggtc cccagccata
      781 cctgagattg gcacctctcc accgtagaaa tggaatggag ctgtcttgcc gtagatttcg
      841 gagttagaac gaacttctgg aagtatagcc ttcggaatgt taaggatttc caaaatctca
      901 tcatcccatt tgagttcttt aatgttataa agcatggtac gagctgcatt tgagtagtca
      961 gtcacgtgag ccgcaccgtc agtcaatttc caaaccaacc aagtatcgat agtaccaaag
     1021 agcaattccc ctttttctgc tcgctcttga gcaccttcta catgatccaa aatccaacga
     1081 accttggtag cagagaagta agcatcaata atcaaaccag tcttttcatg gaatttttcc
     1141 acataacctt ggctttttag ttgctcagcc aaaggtgctg tctggcgtga ctgccaaacg
     1201 atagcattgt agataggaag tcctgttttc ttatcccaga caaccgttgt ttcacgttgg
     1261 ttggtaatcc cgattgcctc gatttgattt ggcttgacac cactttcgat gaaagcaccc
     1321 gcaataactg actgaacaga gttccaaatt tcattggcat tgtgctcaac ccaacctgcc
     1381 tgagggaaaa tctgggtaaa ctctttttga ctcgagctaa ccttttcccc ttttttgttg
     1441 aaaatgatgg cacgagaact tgtagttccc tggtcaatgg ccatgatgta tttttcttgt
     1501 gacatgtact gtcctcctga taaagatctt gaggtatttc ctctttgcac ttactagtat
     1561 acaaaataaa gcgctttctt gtttatcaac tttttttaat ttttaaaaaa atgtgaggag
     1621 attgtgtgaa tttcacaaaa aagacctgga ttaaccaagc cttttaagtg aataataact
     1681 gacgaatccc tgccaaatct tccatatcca agtcattttt taaataatag actggggtct
     1741 gttccttctt atgaatcggg ttattggtaa ccagcaaatc ataatgccga ctagggtcat
     1801 aagcttcaat ggtaataaaa cgattgtatt ccaaataccg tttcaaaatg gctgccatcc
     1861 ttgaaaaaac aagaaaacca gatgtcaaat ccagaccaat ccgcatatgc tggccaccgt
     1921 tttcagccat atattcgatt aactggagcc attcccagag aatcttctta tctaaatctg
     1981 tcccctgttg aaaagcaggt agagcatgaa aaatctcctc tgctgtctct ttaaaatcat
     2041 gttccatcag taaatatgga tgacgattct ctaactggta cttgtagcga tcctgtaaaa
     2101 tatagccctt atagagatag acttgagcac aaagctgact gagttcatag gtgacatggt
     2161 cctctgtata atcccccaat agttcctcct tcttcatttc ttgaatcaat tgcgtcagta
     2221 aatctgccaa ctgccctcca aaaccaagaa tatactccat agtatgaaga ggaagaatgc
     2281 gatgagatag gagaaaagag aagaatatca tcatctcacc atcctcaact cctagtggaa
     2341 tatgttgccc agcaaaaaag gacggaacct ttctcagcaa acgaaggtag aaaatattgt
     2401 caaaataccc tcgcattttc tcttctatga cttgaaactg acaagcattg acccgaagac
     2461 gttgttgact gatgtgagcc cagagaacca agtccaattt ctgccccaca gacaaacttg
     2521 caccgcagat ttcctctaaa ttggcaatct cctgttttct ctctggtttc tgcatgtgac
     2581 cttcccattc ctgactcgac cagacctttc ggaaaagaca gaaatagaaa tagcgaatct
     2641 gatgctctgg acctcgccaa cgtccatttt ggatggataa atcaaattct gacaaaatct
     2701 gatttaaacc agccaagtga cgaccaagcg tagcctcgct aatcaccaat tcttgagcca
     2761 gctgatgggc taaaaactgt tggtggtaga gaagataaac caaaatctgg tatttaacag
     2821 cactctccaa aaacaagctc cgaatatctc tccccttggt agctgcaccg atagacagac
     2881 gcagattttc atcttctgag tgaatagtca gttctaagcc actatccaaa gccttgtcat
     2941 tgagcagggt gacatatttg gttagggttg cttttgaaaa tcctgtttct tccattacag
     3001 ccttgacggt cgtctgagac tcttgtaata gaaaggaaag gattgaaaat tgaccacact
     3061 cggctttctc catcaaatct cctaaataca tttgttaccc ctttcatttt ctaccttaag
     3121 catagcataa atcttacaaa tgctaaaata atctgtttct ctttttattt tcatgctgat
     3181 ttcctggtcc attatcctga aaatcagtaa acacacggct ccccctttgg gcatttttat
     3241 gctaaaatag tagctatgga taaaattatt aaaactatat cagaaagcgg agcctttcgt
     3301 gcttttgtcc ttgacagcac ggaaaccgtc cgcactgctc aagaaaaaca ccaaacccaa
     3361 gctagctcaa ctgtagcgct tggtcgaact cttatcgcta gccagattct cgcagccaat
     3421 gaaaaaggaa ataccaaact tacagttaag gtgttgggat ctagctctct aggtgctatt
     3481 atcaccgtcg ctgataccaa ggggaacgtc aaaggctacg ttcaaaatcc tggtgttgac
     3541 atcaaaaaga ctgcgactgg tgaagtccta gtcggacctt ttgttggaaa tggtcaattc
     3601 ctcgttatca cagactacgg tactggaaat ccttacaact ctataactcc cctcatctct
     3661 ggagaaatcg gtgaagacct tgccttttac cttactgaaa gccaacaaac gccttcagcg
     3721 gtcggcctca atgtcctttt ggacgaggaa gacaaggtca aggttgcagg tggtttccta
     3781 gttcaagtct tgccaggagc caagaaagaa gagattgctc gctttgaaaa acgcatccaa
     3841 gaaatgccag ctatctctac tcttctcgaa agcgacgacc atatcgaagc cctcctcaag
     3901 gctatctacg gggacgaagc ctacaagcgt ctttctgaag aagaaatccg tttccaatgt
     3961 gactgtagcc atgaacgctt tatgaacgct cttgccagcc ttccaagctc agacttacag
     4021 gaaatgaaag aggaagacca cggggcagaa atcacttgtc aattctgcca aactacttac
     4081 aactttgatg aaaaggacct ggaggaactc attcgtgaca aatcttaata caccttttat
     4141 gattggcaat gttgagattc ccaatcgtac cgttttagcg cctatggctg gcgtgaccaa
     4201 ctcagccttt cgtaccatcg caaaagagct cggagctgga ctcgttgtaa tggaaatggt
     4261 ctctgacaag ggaatccaat acaacaacga aaaaaccctg cacatgcttc atatcgatga
     4321 gggcgaaaac cctgtctcta tccaactttt tggtagcgat gaagacagcc tagcacgcgc
     4381 agcagaattc atccaagaaa acaccaagac cgatatcgtc gatatcaaca tgggctgccc
     4441 tgtcaacaaa atcgtgaaga acgaagctgg tgctatgtgg ctcaaggatc ctgacaagat
     4501 ctactctatc atcaacaagg tccagtctgt ccttgatatc ccacttactg tcaaaatgcg
     4561 taccggctgg gcggacccat ctctggcagt agaaaatgct ctcgctgctg aggctgcagg
     4621 tgtttctgcc ctcgccatgc atggccgtac ccgtgaacaa atgtatactg gccacgcaga
     4681 ccttgagacc ctttacaagg ttgcccaagc tctaaccaag attccattca tcgccaacgg
     4741 tgatatccgt actgtccaag aagccaagca acgcatcgaa gaagttggtg ctgacgcagt
     4801 catgattggc cgagctgcca tgggaaatcc ttacctcttc aaccaaatca accattactt
     4861 tgaaacagga gaaatcctac ctgatttgac ctttgaagac aagatgaaga tcgcctacga
     4921 acacttgaaa cgattgatta acctcaaagg agaaaacgtc gcagttcgtg aattccgcgg
     4981 cctcgctcct cactatctcc gtggaacatc tggcgctgcc aaactccgtg gagccatttc
     5041 gcaagctagc accctagcag agattgaagc cctcttgcaa ttggagaagg catagtctta
     5101 catctacaac tcagaaaaac ggcaaagctc aaaatgaact accccaaaaa agttagacag
     5161 aaaaaatcta acttttgggg tgtttttatt atgaaactaa cttatgatga taaagttcag
     5221 atctatgaac ttagaaaaca aggatatagc ttagagaagc tttcaaataa atttgggata
     5281 aataattcta atattaggta catgattaaa ttgattgatc gttacggaat agagttcgtc
     5341 aaaaaaggaa aaaatcgtta ctattttcct gatttaaaac aagaaatgat taataaagtc
     5401 ttacatgaag gctgaactaa agatagagtt tctcttgaat acggcctccc aagtcgtacg
     5461 atacttctta attggctagc acaatacaag aaaaacgggt atactattgt tgagaaaaca
     5521 agagggagag tacctaaaat gggacgtaag ccacaaaaga gacctgaaga gaggacagaa
     5581 ttagaacgtc ttcaagcaga aaatgagtac ctgagagcgg agaatgctat cctaaaaaag
     5641 ttaagagaac tccgatggaa ggaggaaaaa gagaaagaag aaagacagaa attgttcaag
     5701 aattaatgac tgagttttcg ttagatattc ttctaaaagc cattaaacta gctcgttgga
     5761 cctactacta tcacttgaaa cagctagaca aaacagataa ggaccaagag cttaaagctg
     5821 aaattcaatc catttttatc gaacacaagg gaaattatgc ttatcgccgg gttcatttag
     5881 aactaagaaa tcgtgcttat ctggtaaatc ataaaagagt tcaaggcttg at
//
LOCUS       DBEROU010000072         5296 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.072, whole genome shotgun sequence.
ACCESSION   DBEROU010000072 DBEROU010000000
VERSION     DBEROU010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5296
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.072"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            30..835
                     /locus_tag="ACL8CL_002025"
                     /pseudo
     CDS             30..835
                     /locus_tag="ACL8CL_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
     gene            857..1596
                     /locus_tag="ACL8CL_002026"
                     /pseudo
     CDS             857..1596
                     /locus_tag="ACL8CL_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            1609..2775
                     /locus_tag="ACL8CL_002027"
     CDS             1609..2775
                     /locus_tag="ACL8CL_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HGS1331197.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIGRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVDKDGKIVFSTTFDYV
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMEQMIADFGQRQE"
     gene            3035..3160
                     /locus_tag="ACL8CL_002028"
     CDS             3035..3160
                     /locus_tag="ACL8CL_002028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331198.1"
                     /translation="MKIKEQTRKLAAGCSKHCFEVVDRTDRVSIIYLRQGEADVV"
     gene            3283..4776
                     /locus_tag="ACL8CL_002029"
     CDS             3283..4776
                     /locus_tag="ACL8CL_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HGS1331199.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            4789..>5296
                     /locus_tag="ACL8CL_002030"
     CDS             4789..>5296
                     /locus_tag="ACL8CL_002030"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1331200.1"
                     /translation="MKKVKLGEILSLKKGKKATVLAEQTTLSQRYIQIDDLRNNNNLK
                     FTESLNMTEALPDDILIAWDGANAGTVGYGLSGAVGSTITVLKKNERYKEKIISDYLG
                     VFLESKSQYLRDHSTGATIPHLNKNILLDLQLELLGIEEQENIICILNTIKRLITKRK
                     LQLDELNLL"
BASE COUNT         1687 a          936 c         1124 g         1549 t
ORIGIN      
        1 aaattttgaa tatagagagt tttctgacaa tgaatcaatc ctacttttat ctaaaaatga
       61 aagaacacaa actcaaggtt ccttatacag gtaaggagcg ccgtgtacgt attcttcttc
      121 ctaaagatta tgagaaagat acagaccgtt cttatcctgt tgtatacttt catgacgggc
      181 aaaatgtttt taatagcaaa gagtctttca ttggacattc atggaagatt atcccagcta
      241 tcaaacgaaa tccggatatc agtcgcatga ttgtcgttgc tattgacaat gatggtatgg
      301 ggcggatgaa tgagtatgcg gcttggaagt tccaagaatc tcctatccca gggcagcagt
      361 ttggtggtaa gggtgtggag tatgctgagt ttgtcatgga ggtggtcaag ccttttatcg
      421 atgagaccta tcgtacaaaa gcagactgcc agcatacggc tatgattggt tcctcactag
      481 gaggcaatat tacccagttt atcggtttgg aataccaaga ccaaattggt tgcttgggcg
      541 ttttttcatc tgcaaactgg ctccaccaag aagcctttaa ccgctatatc gagtgccaga
      601 aactatcgcc tgaccagcgc atcttcatct atgtaggaac agaagaagca gatgatacag
      661 acaagacctt gatggatggc aatatcaaac aagcctatat cgactcgtcg ctttgctatt
      721 accatgattt gatagcaggg ggagtacatc tggataatct tgtgccatcc atagtgaaat
      781 cccttggtca gaaaatctac cagattgtct gagatttttt gcagaaaaat ggtaagttaa
      841 gaaaggaaaa aacgaaatgc atattgaaca tcttagccac tggagtggtc atcctaaccg
      901 tgaaatgtac cttaaccgtt atggacatgg tgggattcca gttgtggtct ttgcttcatc
      961 aggtggtagt cacaacgaat actatgattt tggcatgatt gatgcctgtg cttcctttat
     1021 cgaggaaggc cgtgtccagt tctttaccct atctagtgtg gatagtgaga gctggttggc
     1081 tacttggaaa aatgctcatg accaagcgga aatgcaccgt gcctacgaac gttatgtgat
     1141 tgaggaggcc attcttttat caagcacaag acaggttggt ttgatggcat gatgacgaca
     1201 ggttgctcta tgggagccta tcatgcactt aatttcttcc tccagcatcc agatgtcttt
     1261 accaaagtga ttgctctcag tggtgtttac gacgcacgtt tctttgtcgg tgattactac
     1321 aacgatgatg ctatttacca aaactcgcca gtagattata tttggaacca aaacgacggc
     1381 tggtttattg accgttaccg tcaggcagag attgtgctgt gtacggggct tggagcctgg
     1441 gaacaagatg gtttgccatc cttttacaag ctcaaagaag cctttgacaa gaaacaaatt
     1501 ccagcctggt ttgctgaatg gggacatgat gtcgcccatg actgggaatg gtggcgtaaa
     1561 caaatgcctt atttcctcgg taatctctat ttataaaagg agttacctat gaattacctt
     1621 gttatttctc cctactatcc acaaaacttt caacagttta ccatcgaact agctaataaa
     1681 ggcatcacag tcttgggaat tggtcaagag tcttacgagc aattggatga gcccttgcgc
     1741 aatagcttga ccgagtattt tcgtgttgat aatcttgaga acatagatga agtcaaacgt
     1801 gcagttgctt ttctctttta taaacatggt ccaattggcc gcatcgagtc tcacaatgaa
     1861 tactggcttg agctagacgc aacactcaga gaacaattca atgtttttgg tgccaaacca
     1921 gaggatctca aaaagacgaa atataagtct gaaatgaaga aacttttcaa aaaagcaggt
     1981 gttcctgtgg tacctggagc tgttatcaag acggaagcag atgttgatca agcagtgaaa
     2041 gaaatcggtc ttccaatgat tgccaaacct gataatggag tgggagcagc cgcaaccttt
     2101 aaacttgaga cagaagacga tatcaatcac ttcaagcaag aatgggacca ttcaaccctt
     2161 tatttctttg aaaaatttgt cacttccagc gaaatctgta cctttgacgg gctcgtggac
     2221 aaggatggaa agattgtctt ctcaacaacc tttgactacg tctatacacc gcttgacctc
     2281 atgatttata agatggacaa ttcttattat gtgctcaagg atatggatcc taaactgcgc
     2341 aagtatgggg aagcaattgt caaagaattt ggtatgaaag aacggttttt ccatattgag
     2401 ttcttccgtg agggggacga ttatattacc atcgagtaca ataaccgccc tgcaggtggt
     2461 tttaccattg atgtttataa ctttgctcat tccttggacc tttatcgtgg ctatgcagct
     2521 attgtcgcag gagaggagtt cccggcgtca gactttgaaa ctcagtattg tttggctact
     2581 tctcgccgtg caaatgctca ctatgtttat tcagaagagg atttgcttgc caaatatagc
     2641 cagcagttca aggttaaaaa agtcatgcca gctgccttcg cggaacttca aggagattac
     2701 ctgtatatgc tgaccactcc gagtcgacaa gaaatggagc agatgattgc agatttcgga
     2761 caacgtcaag aataagaact atcggattaa ggaaattcac tcccttaatc cttttgtttt
     2821 gtctggtaaa aaataagagc atcctaacaa ggtagctatc ataaaacttg ttcgataact
     2881 atttgaagca ggattaggtg gctagaaatt gaattttaac atttcaattg agtcatagta
     2941 ttgtgttttc gtatccttaa atcagctaaa aggatccatg acgacaccta tacgatatag
     3001 ttttcaggat accaaacaag tctattaata ctcaatgaaa atcaaagagc aaactaggaa
     3061 gctagccgca ggttgctcaa aacactgttt tgaggttgtg gatagaactg acagagtcag
     3121 tatcatatac ctacggcaag gtgaagctga cgtggtttga agagattttc gaagagtata
     3181 aaatattcag gtgacgcata gatatagtta attgaagctt tgtttgaaat ctgataaaat
     3241 aatgatatta ctaagttcta aaaactaaag aaaagggaag atatgattac aggcgaatta
     3301 aaaaataaaa tcgatcagct gtgggaaatt ctttggacag aaggaaacgc aaatccttta
     3361 acaaatattg aacagttgac ttatctctta tttatgaaag atttggatag tgtcgagctt
     3421 ggacgtgaaa gtgatgctga atttctaggg attccttatg agggagtttt tccaaaagat
     3481 aaacctgaat accgttggtc aacttttaaa aatataggag atgctcagga agtttatcgt
     3541 ttaatgactc aggagatttt tccgtttatt aaaaatctca agggggatac agatgataca
     3601 gccttttcac gatatatgcg agaagctatt tttcaaataa ataaacctgc tacgcttcaa
     3661 aaggcaattt ctatcttaga tgtttttcca actaggggat tagatgtaga ttttgataat
     3721 gacaaacaaa gtattactga tatcggagat atctatgaat atctgttatc aaaattgtcg
     3781 accgcaggta aaaatggaca gttccgtaca cctcgtcaca tcatcgatat gatggttgag
     3841 ttgatgcaac cgactatcaa agatatcatc tcagatcccg ctatgggttc tgctggcttc
     3901 ttagtatctg ctagccgtta cttaaagcgt aagaaagatg aatgggaaac caatacagat
     3961 aatatcaatc attttcataa tcagatgttt catggaaatg atacggatac gactatgttg
     4021 agacttgggg cgatgaacat gatgctacat ggagtagaaa atccacaaat cagttacctt
     4081 gactcgctgt ctcaagataa tgaagaagcc gataaatata ctttggtttt agcaaatcct
     4141 ccttttaagg gctcacttga ctacaattca acctctaatg accttcttgc aaccgtaaaa
     4201 accaaaaaaa cagaattact ctttctttct cttttcttgc gaactttaaa accaggtgga
     4261 cgagcagcag ttatcgtacc tgatggtgtc ctttttggtt cgtctaaagc tcataaagga
     4321 attcgtcagg aaattgtaga gaatcataag cttgatgctg taatctcaat gcctagtggt
     4381 gtgttcaagc cttatgctgg agtttcaact gctattctca tctttacaaa aactggtaat
     4441 ggtggtactg acaaagtctg gttttacgat atgaaagcgg atggtttaag tttggatgat
     4501 aagcgacaac cgattagcga caatgatatt ccagatatta tcgaacgctt tcatcatctt
     4561 gaaaaagaag cagaacgtca gagaacggat caatctttct ttgttccagt tgctgagata
     4621 aaggaaaatg attatgattt gtctatcaat aaatataaag agattgagta tgaaaaagtt
     4681 gagtatgaac caacagaggt catcttaaag aaaatcaatg atttagaaaa agaaattcaa
     4741 gctggcttgg ctgaattaga aaaattactc aagtagggag gtggctgtat gaaaaaagtg
     4801 aagttggggg aaatcttatc tctaaaaaaa ggaaagaaag ccactgtact tgctgaacaa
     4861 acaactctaa gccaacgtta tattcaaata gatgatttaa gaaataataa taatttaaaa
     4921 ttcactgaaa gtttaaatat gactgaagca ctcccagatg atattctaat agcatgggat
     4981 ggtgctaatg caggaacagt tggttatgga ttatcgggag ctgttggtag tacaattacg
     5041 gtcttaaaaa agaatgagcg atacaaagaa aaaattatat cagattactt gggagtcttt
     5101 ttggaaagta aatcgcagta tttacgagat cattcaacag gtgcaacaat tcctcattta
     5161 aacaagaata tattacttga tttacaatta gaattgctag gtatcgaaga acaagagaac
     5221 attatctgta ttcttaatac gattaaaagg cttattacta aaagaaaatt gcagttagat
     5281 gaactaaact tgctcg
//
LOCUS       DBEROU010000073         5212 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.073, whole genome shotgun sequence.
ACCESSION   DBEROU010000073 DBEROU010000000
VERSION     DBEROU010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5212
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.073"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(360..1088)
                     /locus_tag="ACL8CL_002031"
     CDS             complement(360..1088)
                     /locus_tag="ACL8CL_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HGS1331201.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
     gene            1246..2655
                     /locus_tag="ACL8CL_002032"
     CDS             1246..2655
                     /locus_tag="ACL8CL_002032"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HGS1331202.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTEDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWPERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYDNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            2673..3968
                     /locus_tag="ACL8CL_002033"
     CDS             2673..3968
                     /locus_tag="ACL8CL_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HGS1331203.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMEG"
     gene            3973..4281
                     /locus_tag="ACL8CL_002034"
     CDS             3973..4281
                     /locus_tag="ACL8CL_002034"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HGS1331204.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            4278..4586
                     /locus_tag="ACL8CL_002035"
     CDS             4278..4586
                     /locus_tag="ACL8CL_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HGS1331205.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKVEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1756 a          879 c          949 g         1628 t
ORIGIN      
        1 tgggagattg aaattcattt ctagcaattt gttagaagtc acagtctact attctaacca
       61 cagtatacta tatgtgttac aagaaaagac tcaaaaactt ctcgtcagtg taagaatgtt
      121 tgaaacacta tttaaaacta aataactata agaagtagat caggtgaaca atttcagtac
      181 ctactctata agctatacga agatattttt caacatgatg gataaaacga tcactctttt
      241 aaagccatct tatttagaaa agagaaacca tttaagcata gatgggacct tcttgccctg
      301 ttttaaattt ttcttaaact agagtctgta aaagattggc ttcattttca cattttctcc
      361 taggaggaat gtcgtaatgc aaaagaatta aattcaaata aatcatagcg atgacgacta
      421 atcgagtact caaacggagt tccgttatcc aaataagcca cttgttctac ttcaaatacc
      481 ggttccgttg gaaggagctg caagtaatgt tgctcatttt ctgaactaga agaagctcgc
      541 aagatttttg tagcactggc aatatgcaat cctaactttc cttcaatgta attgtaaatc
      601 gatttttgta aaatatcttc agtaatacct ggaataacat cggtactcat ataagtttgt
      661 tccagaacat aaggtttacc gtcaataata cgcagacggt aaatattata aactggactc
      721 tgcaaagcga cctgtagttt ttctgctaaa aattcatttg caaattctaa tttgaagtga
      781 ataatcctcg agtcaattgt agaatgagca tcttcagata tttttgtcag tccccggata
      841 tctctttctg gaacgattaa ttttcttttt gagctgccac gagagagaac aaaggttccc
      901 tgcccacgtt ttctaaaaat taagccctca gaaactaaca agtctaaagc ttttttgatc
      961 gtcattcgac tacaggcaaa ctgtttagct aatgtcattt catcaggcaa ttgttcgttc
     1021 atggtatatt tattacaaag aatcttttca cgaatctcat tgtaaataaa taaatatttt
     1081 gtactcattt aatctggttc tctctataat attctaatag tagtataaca aagttcaaga
     1141 aatgaggcaa acacaagata tgtatcaaca atttttaaaa atgtctagac aaattttaaa
     1201 attatgctat actttatgta tagaataata gaaaggaggt tgagcatgaa aaaaaagtta
     1261 gtatttccta atctgttttg gtggggagct gcttctagcg gacctcagac agaaggtcaa
     1321 tatggaaaag tacatgaaaa tgtgatggac tactggttca aaacgcatcc agaagatttt
     1381 ttcgataatg tcggacctct tgtagccagt aacttttttc atacttacac cgaagatttc
     1441 cacttgatga aggaaattgg agttaattct ttccgcactt ccatccaatg gagtcgactc
     1501 atcaagaatt tagagacagg tgagcctgat ccaaaaggta ttgctttcta caatgccatc
     1561 attgaagaag ctaaaaagaa ccagatggat cttgtgatga atttacatca ttttgattta
     1621 ccagtggaac ttcttcaaaa atacggtggt tgggaaagca aacatgtagt ggagttattc
     1681 gtgaagtttg ccaagactgc tttcacatgc tttggagata aggttcatta ctggacaact
     1741 ttcaatgagc caatggtcat tccagaagca ggatacttat atgctttcca ttatccaaat
     1801 ctaaaaggaa agggaaaaga ggccgtacaa gtcatctata atctaaacct tgctagtgca
     1861 aaagtgattc aactatatcg ctcattagaa cttgatggaa agattgggat tattttaaac
     1921 ttgacacctg cttatccaag aagtaattct ccagaagact tagaagcaag tcgatttaca
     1981 gaagacttct ttaacaaagt cttcttgaat ccagctgtta aaggaacttt cccagaaaga
     2041 ttggtaaaac agctagagag agatggcgtg ttatggagtc ataccgaaaa agagcttcaa
     2101 ctgatgaaat caaatacggt tgattttctt ggagtaaact actaccatcc aaaacgtgtt
     2161 caagcacaag caaatcctga ggaatatcag acgccctgga tgccagacca atacttcaaa
     2221 gagtatgaat ggccggagcg tcgcatgaat ccatatcgtg gttgggaaat ttttccgaaa
     2281 gccatttatg atattgctat gattgtgaag gaagaatatg ataatattcc atggtttatc
     2341 agtgaaaacg gaatgggtgt tgaaaacgaa gcacggttta tcgatgaaaa tggagttatc
     2401 gatgacgtgt atcgtattga attttatgaa gaacatttaa gatggctaca taaagccatt
     2461 gaagagggaa gtcactgttt tggataccac gcttggaccg catttgattg ctggtcttgg
     2521 aataatgcat ataagaatcg ttacggattt atctccgttg atttagaaac gcaaaagaga
     2581 accatcaaga gctcaggaag atggtatcgc aaagtaagtg acaataacgg ttttgaagta
     2641 gaaattgagg agtaaagaga ggagatattc agatgaaaaa ctcaaaattt atagatcaat
     2701 ttgccacctt tgctggtaaa ctagggaacc aaattcattt aaaaacccta agagatgcat
     2761 tcgtaacagt aatgccatta tatattttgg caggtttaat cgttcttttg aacaacacgg
     2821 tatttaagtg gattttccaa ggggatacat taacaagatt ccaatattgg ggaataacaa
     2881 ttgcaaacgg tactttaagt atttcaggta tgattattgc tgtaatggtt ggttatttct
     2941 tagctaaaaa cagagatttc gaaaacccgt tagcagcatc aatgctatca ttagtttctt
     3001 taattgtgat gatgccaaat acagtttctg tagttcctga cggagcaaaa gatgcggtaa
     3061 acatttcagg tgttctttca ttcaacaaca caggtacagg cgcaatgttc gccggggtta
     3121 tcgtagcgat tattgcaaca gaattattca ttgaattatc aaacgttaaa gctttacaaa
     3181 tgaaccttgg tgaaaatatt ccaccagctg ttagtagatc atttagcgta ttacttccag
     3241 tcatgaccgt catctcctta tttggggttg tttcagcatt attattcaat ataactggaa
     3301 tgaacttaat ctcaatcatt acaatcttta ttcaagaacc aattcgtcat attggtacaa
     3361 gcttaatcgg ggtcattatt atttactctt taggaaatat gttatggcta tttggtattc
     3421 accaagcagt tatttacagt gccatcctag aaccattact attaattaac attactgaaa
     3481 acatcactgc agcaaataat ggacaagcca ttccacacat catcaaccta tcacaaatac
     3541 aaacattcgc tttaatgggt ggtagtggat ctacattatg tttattaata gcaacattct
     3601 tagtgagtcg caatgctgtc tctaaaaacg tggctaaatt atcttttgga cctggtatct
     3661 tcaatatcaa tgaaccagta ttattcggtt acccaatcgt ttataacatt tcattagcta
     3721 ttccatttat cacagttcca gtccttggta ttttaatcag ctacttagca acagttacag
     3781 gattcatgag tcctgcattt atacaagttc cttggactac accagtattc ttaaatgcat
     3841 ggttagcaac agcaggggac gtgagagcag ttctagttca attcatcatc tttgcacttg
     3901 gagttcttct atacattcca tttatcaaag ttaatgacaa agttgttgaa caagaaatgg
     3961 aaggttaaga ttatgaaaca aattttatta gtatgtaatg cgggaatgtc gacaagtatg
     4021 cttgttaaaa aaatgcaaca aagtgcgact gaacgtggga ttgaaatctc tatccaagca
     4081 aaatcaatga cagaagctaa aaagaatatc catgaagcag atgtcattct aattgggcca
     4141 caaattcgtt atgagttact tgctgtgaaa gaaattgcag gaaatatccc tgtagatacc
     4201 attgatatgc gggattacgg aatgatgaat ggagcaaaag ttctggaaca agcacttgca
     4261 tggataggag aaataagatg acagaagaaa tggaatatat ctgttttcag ctcattgcca
     4321 atagtggagc agccaaatca tcctttattg aagccatcca actagctaag gcaggaaatt
     4381 taaaagaagc gaaaattaaa gttgaagaag cggaagactc cttagtagaa gcgcacaaga
     4441 ttcactctaa tctcatacaa aaagaagcta ctggagaaaa aataggattc tccttgctat
     4501 ttatgcatgc agaagaccaa atggcttcaa cagagattat tcagttgcta tctaaagaat
     4561 tcatcgatct ctatcaaaat aaataggaac actgtcattc ccatctgtct ttattaatga
     4621 cgactacatt gtaggaacct ccaaaagttg gacaaaatga ttgaaaggat tggatcctgc
     4681 attacacaag atccaatcct tttcttttac tctagttttt ataatatgtt gaatctagaa
     4741 tagtccactg tagtttctaa aaaattgctg gaaatggatg ttaagctcca atttatttgt
     4801 ttatatctta tttcagttta ctatactagc ttattattct agtgaaaaat tgaatgagcc
     4861 actatttatt taatatgtat gatgagggaa tgactcaaaa acttcttgtc gatataagaa
     4921 tatctgagat agtattttaa aacgaaagga ctataaaagc tcatttcctt cgcacttatt
     4981 ttatagagat ctatcattat actctttaga aatcaaaaag caacacacct tgtcatcgtt
     5041 ttatcgtaaa tctgttaatg agggggctag tcttatctgc aatctcaaat cagtgttttg
     5101 agctgaatgg cactcgtttt ctagtttgct ctttgatttt aattgggcct tagtaaatac
     5161 tactcttaaa ttaaactata gtgaattgaa ataagatgtg aacaactcta tc
//
LOCUS       DBEROU010000074         5031 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.074, whole genome shotgun sequence.
ACCESSION   DBEROU010000074 DBEROU010000000
VERSION     DBEROU010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 5031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.074"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1637
                     /locus_tag="ACL8CL_002036"
     CDS             <1..1637
                     /locus_tag="ACL8CL_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HGS1331206.1"
                     /translation="LVFDPAQITSRTARGVAVPHGNHYHFIPYEQMSELEERIARIIP
                     LRYRSNHWVPDSRPEEPSPQLTPEPSPSPQPAPNPQPAPSNPIDEKLVKEAVRKVGDG
                     YVFEENGVSRYIPAKDLSAETAAGIDSKLAKQESLSHKLGTKKTDLPSSDREFYNKAY
                     DLLARIHQDLLDNKGRQVDFEALDNLLERLKDVSSDKVKLVEDILAFLAPIRHPERLG
                     KPNAQITYTDDEIQVAKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDS
                     LSEAERAAAQAYAKEKGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNL
                     QYTVEVKNGSLIIPHYDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERP
                     HSDNGFGNASDHVRKNKADQDSKPDEDKEHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVEHSVINAKIAEAEALLEKVTDSSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKKSQPAPIQ"
     gene            1847..4966
                     /gene="phtE"
                     /locus_tag="ACL8CL_002037"
     CDS             1847..4966
                     /gene="phtE"
                     /locus_tag="ACL8CL_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HGS1331207.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNKKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1819 a          887 c         1005 g         1320 t
ORIGIN      
        1 gccttgtttt cgacccagcg caaatcacaa gtcgaacagc cagaggtgta gctgtccctc
       61 atggtaacca ttaccacttt atcccttatg aacaaatgtc tgaattggaa gaacgaattg
      121 ctcgtattat tccccttcgt tatcgttcaa accattgggt accagattca agaccagaag
      181 aaccaagtcc acaactgact ccagaaccta gtccaagtcc gcaacctgca ccaaatcctc
      241 aaccagctcc aagcaatcca attgatgaga aattggtcaa agaagctgtt cgaaaagtag
      301 gcgatggtta tgtctttgag gagaatggag tttctcgtta tatcccagcc aaggatcttt
      361 cagcagaaac agcagcaggc attgatagca aactggccaa gcaggaaagt ttatctcata
      421 agctaggaac taagaaaact gacctcccat ctagtgatcg agaattttac aataaggctt
      481 atgacttact agcaagaatt caccaagatt tacttgataa taaaggtcga caagttgatt
      541 ttgaggcttt ggataacctg ttggaacgac tcaaggatgt ctcaagtgat aaagtcaagt
      601 tagtggaaga tattcttgcc ttcttagctc cgattcgtca tccagaacgt ttaggaaaac
      661 caaatgcgca aattacctac actgatgatg agattcaagt agccaagttg gcaggcaagt
      721 acacaacaga agacggttat atctttgatc ctcgtgatat aaccagtgat gagggggatg
      781 cctatgtaac tccacatatg acccatagcc actggattaa aaaagatagt ttgtctgaag
      841 ctgagagagc ggcagcccag gcttatgcta aagagaaagg tttgacccct ccttcgacag
      901 accatcagga ttcaggaaat actgaggcaa aaggagcaga agctatctac aaccgcgtga
      961 aagcagctaa gaaggtgcca cttgatcgta tgccttacaa tcttcaatat actgtagaag
     1021 tcaaaaacgg tagtttaatc atacctcatt atgaccatta ccataacatc aaatttgagt
     1081 ggtttgacga aggcctttat gaggcaccta aggggtatac tcttgaggat cttttggcga
     1141 ctgtcaagta ctatgtcgaa catccaaacg aacgtccgca ttcagataat ggttttggta
     1201 acgctagcga ccatgttcgt aaaaataagg cagaccaaga cagtaaacct gatgaagata
     1261 aggaacatga tgaagtaagt gagccaactc accctgaatc tgatgaaaaa gagaatcacg
     1321 ctggtttaaa tccttcagca gataatcttt ataaaccaag cactgatacg gaagagacag
     1381 aggaagaagc tgaagatacc acagatgagg ctgaaattcc tcaagtagag cattctgtta
     1441 ttaacgctaa gatagcagag gcggaggcct tgctagaaaa agtaacagat tctagtatta
     1501 gacaaaatgc tatggagaca ttgactggtc taaaaagtag tcttcttctc ggaacgaaag
     1561 ataataacac tatttcagca gaagtagata gtctcttggc tttgttaaaa aaaagtcaac
     1621 cggctccgat acagtagtaa aatgaatgga gcatatttta tggagaagta acctttcgtg
     1681 ttacttctct tttttagaaa aacgtaacag aaacttgaca aaagtaattt taaatagtaa
     1741 actatttact ggttaattaa atggttaaat aaccggttta gaaaactatt taataaagta
     1801 aaagaagttg agaaaaaact tcatcattta ttgaaatgag ggatttatga aatttagtaa
     1861 aaaatatata gcagctggat cagctgttat cgtatccttg agtctatgtg cctatgcact
     1921 aaaccagcat cgttcgcagg aaaataagga caataatcgt gtctcttatg tggatggcag
     1981 ccagtcaagt cagaaaagtg aaaacttgac accagaccag gttagccaga aagaaggaat
     2041 tcaggctgag caaattgtaa tcaaaattac agatcagggc tatgtaacgt cacacggtga
     2101 ccactatcat tactataatg ggaaagttcc ttatgatgcc ctctttagtg aagaactctt
     2161 gatgaaggat ccaaactatc aacttaaaga cgctgatatt gtcaatgaag tcaagggtgg
     2221 ttatatcatc aaggtcgatg gaaaatatta tgtctacctg aaagatgcag ctcatgctga
     2281 taatgttcga actaaagatg aaatcaatcg tcaaaaacaa gaacatgtca aagataatga
     2341 gaaggttaac tctaatgttg ctgtagcaag gtctcaggga cgatatacga caaatgatgg
     2401 ttatgtcttt aatccagctg atattatcga agatacgggt aatgcttata tcgttcctca
     2461 tggaggtcac tatcactaca ttcccaaaag cgatttatct gctagtgaat tagcagcagc
     2521 taaagcacat ctggctggaa aaaatatgca accgagtcag ttaagctatt cttcaacagc
     2581 tagtgacaat aacacgcaat ctgtagcaaa aggatcaact agcaagccag caaataaatc
     2641 tgaaaatctc cagagtcttt tgaaggaact ctatgattca cctagcgccc aacgttacag
     2701 tgaatcagat ggcctggtct ttgaccctgc taagattatc agtcgtacac caaatggagt
     2761 tgcgattccg catggcgacc attaccactt tattccttac agcaagcttt ctgccttaga
     2821 agaaaagatt gccagaatgg tgcctatcag tggaactggt tctacagttt ctacaaatgc
     2881 aaaacctaat gaagtagtgt ctagtctagg cagtctttca agcaatcctt cttctttaac
     2941 gacaagtaag gagctctctt cagcatctga tggttatatt tttaatccaa aagatatcgt
     3001 tgaagaaacg gctacagctt atattgtaag acatggtgat catttccatt acattccaaa
     3061 atcaaatcaa attgggcaac cgactcttcc aaacaatagt ctagcaacac cttctccatc
     3121 tcttccaatc aatccaggaa cttcacatga gaaacatgaa gaagatggat acggatttga
     3181 tgctaatcgt attatcgctg aagatgaatc aggttttgtc atgagtcacg gagaccacaa
     3241 tcattatttc ttcaagaagg acttgacaga agagcaaatt aaggctgcgc aaaaacattt
     3301 agaggaagtt aaaactagtc ataatggatt agattctttg tcatctcatg aacaggatta
     3361 tccaagtaat gccaaagaaa tgaaagattt agataaaaaa atcgaagaaa aaattgctgg
     3421 cattatgaaa caatatggtg tcaaacgtga aagtattgtc gtgaataaag aaaaaaatgc
     3481 gattatttat ccgcatggag atcaccatca tgcagatccg attgatgaac ataaaccggt
     3541 tggaattggt cattctcaca gtaactatga actgtttaaa cccgaagaag gagttgctaa
     3601 aaaagaaggg aataaagttt atactggaga agaattaacg aatgttgtta atttgttaaa
     3661 aaatagtacg tttaataatc aaaactttac tctagccaat ggtcaaaaac gcgtttcttt
     3721 tagttttccg cctgaattgg agaaaaaatt aggtatcaat atgctagtaa aattaataac
     3781 accagatgga aaagtattgg agaaagtatc tggtaaagta tttggagaag gagtagggaa
     3841 tattgcaaac tttgaattag atcaacctta tttaccagga caaacattta agtatactat
     3901 cgcttcaaaa gattatccag aagtaagtta tgatggtaca tttacagttc caacctcttt
     3961 agcttacaaa atggccagtc aaacgatttt ctatcctttc catgcagggg atacttattt
     4021 aagagtgaac cctcaatttg cagtgcctaa aggaactgat gctttagtca gagtgtttga
     4081 tgaatttcat ggaaatgctt atttagaaaa taactataaa gttggtgaaa ttaaattacc
     4141 gattccgaaa ttaaaccaag gaacaaccag aacggccgga aataaaattc ctgtaacctt
     4201 catggcaaat gcttatttgg acaatcaatc gacttatatt gtggaagtac ctatcttgga
     4261 aaaagaaaat caaactgata aaccaagtat tctaccacaa tttaaaagga ataaagcaca
     4321 agaaaactca aaacttgatg aaaaggtaga agaaccaaag actagtgaga aggtagaaaa
     4381 agaaaaactt tctgaaactg ggaatagtac tagtaattca acgttagaag aagttcctac
     4441 agtggatcct gtacaagaaa aagtagcaaa atttgctgaa agttatggga tgaagctaga
     4501 aaatgtcttg tttaatatgg acggaacaat tgaattatat ttaccatcgg gagaagtcat
     4561 taaaaagaat atggcagatt ttacaggaga agcacctcaa ggaaatggtg aaaataaacc
     4621 atctgaaaat ggaaaagtat ctactggaac agttgagaac caaccaacag aaaataaacc
     4681 agcagattct ttaccagagg caccaaacaa aaaacctgta aaaccagaaa actcaacgga
     4741 taatggaatg ttgaatccag aagggaatgt ggggagtgac cctatgttag atccagcatt
     4801 agaggaagct ccagcagtag atcctgtaca agaaaaatta gaaaaattta cagctagtta
     4861 tggattaggt ttagatagtg ttatattcaa tatggatgga acgattgaat taagattgcc
     4921 aagtggagaa gtgataaaaa agaatttatc tgatctcata gcgtaaggaa tagcagtaga
     4981 aaaagtctga atcaaaaatg aagttctctc aaaagttaga aataaaactc t
//
LOCUS       DBEROU010000075         4910 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.075, whole genome shotgun sequence.
ACCESSION   DBEROU010000075 DBEROU010000000
VERSION     DBEROU010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.075"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            200..1745
                     /locus_tag="ACL8CL_002038"
     rRNA            200..1745
                     /locus_tag="ACL8CL_002038"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1797..1869
                     /locus_tag="ACL8CL_002039"
     tRNA            1797..1869
                     /locus_tag="ACL8CL_002039"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1830..1832,aa:Ala,seq:tgc)
     gene            1994..4895
                     /locus_tag="ACL8CL_002040"
     rRNA            1994..4895
                     /locus_tag="ACL8CL_002040"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1383 a         1002 c         1446 g         1079 t
ORIGIN      
        1 atcttcaaaa aagtgttgac aagcgaaagc ggctgtgata tactaatata gttgtcgctt
       61 gagagaagca agtgacaaag acctttgaaa actgaacaag acgaaccaat gtgcagggcg
      121 ctacaacata agttgtagta ctgaacaatg aaaaaaacaa taaatctgtc agtgacagaa
      181 atgagtgaga actcaaactt ttaatgagag tttgatcctg gctcaggacg aacgctggcg
      241 gcgtgcctaa tacatgcaag tagaacgctg aaggaggagc ttgcttctct ggatgagttg
      301 cgaacgggtg agtaacgcgt aggtaacctg cctggtagcg ggggataact attggaaacg
      361 atagctaata ccgcataaga gtagatgttg catgacattt gcttaaaagg tgcacttgca
      421 tcactaccag atggacctgc gttgtattag ctagttggtg gggtaacggc tcaccaaggc
      481 gacgatacat agccgacctg agagggtgat cggccacact gggactgaga cacggcccag
      541 actcctacgg gaggcagcag tagggaatct tcggcaatgg acggaagtct gaccgagcaa
      601 cgccgcgtga gtgaagaagg ttttcggatc gtaaagctct gttgtaagag aagaacgagt
      661 gtgagagtgg aaagttcaca ctgtgacggt atcttaccag aaagggacgg ctaactacgt
      721 gccagcagcc gcggtaatac gtaggtcccg agcgttgtcc ggatttattg ggcgtaaagc
      781 gagcgcaggc ggttagataa gtctgaagtt aaaggctgtg gcttaaccat agtaggcttt
      841 ggaaactgtt taacttgagt gcaagagggg agagtggaat tccatgtgta gcggtgaaat
      901 gcgtagatat atggaggaac accggtggcg aaagcggctc tctggcttgt aactgacgct
      961 gaggctcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca cgctgtaaac
     1021 gatgagtgct aggtgttaga ccctttccgg ggtttagtgc cgtagctaac gcattaagca
     1081 ctccgcctgg ggagtacgac cgcaaggttg aaactcaaag gaattgacgg gggcccgcac
     1141 aagcggtgga gcatgtggtt taattcgaag caacgcgaag aaccttacca ggtcttgaca
     1201 tccctctgac cgctctagag atagagtttt ccttcgggac agaggtgaca ggtggtgcat
     1261 ggttgtcgtc agctcgtgtc gtgagatgtt gggttaagtc ccgcaacgag cgcaacccct
     1321 attgttagtt gccatcattc agttgggcac tctagcgaga ctgccggtaa taaaccggag
     1381 gaaggtgggg atgacgtcaa atcatcatgc cccttatgac ctgggctaca cacgtgctac
     1441 aatggctggt acaacgagtc gcaagccggt gacggcaagc taatctctta aagccagtct
     1501 cagttcggat tgtaggctgc aactcgccta catgaagtcg gaatcgctag taatcgcgga
     1561 tcagcacgcc gcggtgaata cgttcccggg ccttgtacac accgcccgtc acaccacgag
     1621 agtttgtaac acccgaagtc ggtgaggtaa ccgtaaggag ccagccgcct aaggtgggat
     1681 agatgattgg ggtgaagtcg taacaaggta gccgtatcgg aaggtgcggc tggatcacct
     1741 cctttctaag gataaggaac tgcgcattgg tcttgtttag tcttgagagg tcttgtgggg
     1801 ccttagctca gctgggagag cgcctgcttt gcacgcagga ggtcagcggt tcgatcccgc
     1861 taggctccat tggtgagaga tcaccaagta atgcacattg aaaattgaat atctatatca
     1921 aatagtaaca agaaaataaa ccgaaaacgc tgtagtatta ataagagttt atgactgaaa
     1981 ggtcagaaaa taaggttaag ttaataaggg cgcacggtgg atgccttggc actaggagcc
     2041 gacgaaggac gtgacaaacg acgatatgcc ttgggtagct gtaagtaagc gatgatccag
     2101 ggatttccga atgggggaac ccaacaggta atacctgtta cccacatctg ttaaggatgt
     2161 gaggaggaag acgcagtgaa ctgaaacatc taagtagctg caggaagaga aagcaaaagc
     2221 gattgcctta gtagcggcga gcgaaacggc agaagggcaa accgaagagt ttactcttcg
     2281 gggttgtagg actgcaatgt ggactcaaag attatagaag aatgatttgg gaagatcagc
     2341 caaagagagt aatagcctcg tatttaaaat agtctttgta cctagcagta tcctgagtac
     2401 ggcgggacac gtgaaatccc gtcggaatct gggaggacca tctcccaacc ctaaatactc
     2461 cctagtgacc gatagtgaac cagtaccgtg agggaaaggt gaaaagcacc ccgggagggg
     2521 agtgaaatag aacctgaaac cgtgtgccta caacaagttc gagcccgtta atgggtgaga
     2581 gcgtgccttt tgtagaatga accggcgagt tacgttatga tgcgaggtta agttgaagag
     2641 acggagccgt agggaaaccg agtctgaata gggcgcctta gtatcatgac gtagacccga
     2701 aaccatgtga cctacccatg agcaggttga aggtgcggta agacgcactg gaggaccgaa
     2761 ccagggcacg ttgaaaagtg cttggatgac ttgtgggtag cggagaaatt ccaaacgaac
     2821 ttggagatag ctggttctct ccgaaatagc tttagggcta gcgtcgacat tagagattct
     2881 tggaggtaga gcactgtttg ggtgaggggt ccatcccgga ttaccaatct cagataaact
     2941 ccgaatgcca atgaattatg gtcggcagtc agactgcgag tgctaagatc cgtagtcgaa
     3001 agggaaacag cccagaccac cagctaaggt cccaaaataa ttgttaagtg gaaaaggatg
     3061 tggggttgca cagacaacta ggatgttagc ttagaagcag ctattcattc aaagagtgcg
     3121 taatagctca ctagtcgagt gaccctgcgc cgaaaatgta ccggggctaa aacaatttac
     3181 cgaagctgtg gataccttta taggtatggt aggagagcgt tctatgtgtg atgaaggtat
     3241 accgtgagga gtgctggaac gcatagaagt gagaatgccg gtatgagtag cgaaagacag
     3301 gtgagaatcc tgtccaccgt aagactaagg tttccagggg aaggctcgtc cgccctgggt
     3361 tagtcgggac ctaaggagag accgaaaggt gtatccgatg gacaacaggt tgatattcct
     3421 gtactagagt atgtagtgat ggagggacgc agtaggctaa ctaaagcaga cgattggaag
     3481 agtctgtcta agcagtgagg tgtgaattga gtcaaatgct taattctata acattgagct
     3541 gtgatgggga gcgaagttta gtagcgaagt tagtgacgtc acactgccaa gaaaagcttc
     3601 tagcgtttaa acatactcta cccgtaccgc aaaccgacac aggtagtcga ggcgagtagc
     3661 ctcaggtgag cgagagaact ctcgttaagg aactcggcaa aatgaccccg taacttcggg
     3721 agaaggggtg ctgactttaa gtcagccgca gtgaataggc ccaagcaact gtttatcaaa
     3781 aacacagctc tctgctaaat cgtaagatga tgtatagggg gtgacgcctg cccggtgctg
     3841 gaaggttaag aggagtgctt agcgtaagcg aaggtatgaa ttgaagcccc agtaaacggc
     3901 ggccgtaact ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacccgc
     3961 acgaaaggcg taatgatttg ggcactgtct caacgagaga ctcggtgaaa ttttagtacc
     4021 tgtgaagatg caggttaccc gcgacaggac ggaaagaccc catggagctt tactgcagtt
     4081 tgatattgag tgtctgtacc acatgtacag gataggtagg agtctaagag atcgggacgc
     4141 cagtttcgaa ggagacgctg ttgggatact acccttgtgt tatggccact ctaacccaga
     4201 taggtgatcc ctatcggaga cagtgtctga cgggcagttt gactggggcg gtcgcctcct
     4261 aaaaggtaac ggaggcgccc aaaggttccc tcagaatggt tggaaatcat tcgcagagtg
     4321 taaaggtata agggagcttg actgcgagag ctacaactcg agcagggacg aaagtcgggc
     4381 ttagtgatcc ggtggttccg tatggaaggg ccatcgctca acggataaaa gctaccctgg
     4441 ggataacagg cttatctccc ccaagagttc acatcgacgg ggaggtttgg cacctcgatg
     4501 tcggctcgtc gcatcctggg gctgtagtcg gtcccaaggg ttgggctgtt cgcccattaa
     4561 agcggcacgc gagctgggtt cagaacgtcg tgagacagtt cggtccctat ccgtcgcggg
     4621 cgtaggaaat ttgagaggat ctgctcctag tacgagagga ccagagtgga cttaccgctg
     4681 gtgtaccagt tgtcttgcca aaggcatcgc tgggtagcta tgtagggaag ggataaacgc
     4741 tgaaagcatc taagtgtgaa acccacctca agatgagatt tcccatgatt atatatcagt
     4801 aagagccctg agagatgatc aggtagatag gttagaagtg gaagtgtggc gacacatgta
     4861 gcggactaat actaatagct cgaggactta tccaaagtaa ctgagaatat
//
LOCUS       DBEROU010000076         4839 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.076, whole genome shotgun sequence.
ACCESSION   DBEROU010000076 DBEROU010000000
VERSION     DBEROU010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.076"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            240..1685
                     /gene="cps4A"
                     /locus_tag="ACL8CL_002041"
     CDS             240..1685
                     /gene="cps4A"
                     /locus_tag="ACL8CL_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HGS1331208.1"
                     /translation="MSRRFKKSGSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSISVAVLADSEIENVTQLTSVAAPTGTDNENIQKLLADIKSSQNTDLT
                     VNQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFTKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKKILLTTTPRDAYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKMIDLLGGIDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIIDSLQDSIQTNMPLETMINLVNAQLESGGTYKVNSQDLKGTGRMDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1687..2418
                     /gene="cps4B"
                     /locus_tag="ACL8CL_002042"
     CDS             1687..2418
                     /gene="cps4B"
                     /locus_tag="ACL8CL_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4B"
                     /protein_id="HGS1331209.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLAEAYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMHTSYRQIHTGLSNILMLGITPVIAHIERYNALENNEKRVRELIDMGCYT
                     QINSYHVSKPKFFGEKYKFMKKRARYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKELFVDNPRKIIMDQLI"
     gene            2427..3119
                     /gene="cpsC"
                     /locus_tag="ACL8CL_002043"
     CDS             2427..3119
                     /gene="cpsC"
                     /locus_tag="ACL8CL_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HGS1331210.1"
                     /translation="MKEQNTLEIDVLQLFRVLWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQGEKSGLTSQDLQAGSYLVKDYREIILSQDVLEEVVSDLKLD
                     LTPKGLANKIKVTVPADTRIVSVSVNDRVPEEASRIANSLREVAAKKIISITRVSDVT
                     TLEEARPAISPSSPNLKRNTLIGFLAGVIGTSVIVLLLELLDTRVKRPEDIEDTLQMT
                     LLGVVPNLNKLK"
     gene            3129..3812
                     /gene="cpsD"
                     /locus_tag="ACL8CL_002044"
     CDS             3129..3812
                     /gene="cpsD"
                     /locus_tag="ACL8CL_002044"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HGS1331211.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVS
                     PGEGKTTTSVNIAWSFARAGYKTLLIDGDIRNSVISGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEANKRDVQKAQQQLKQTGKLFLGVVFNKLDISVDKYGVY
                     GFYGNYGKK"
     gene            3828..>4839
                     /locus_tag="ACL8CL_002045"
     CDS             3828..>4839
                     /locus_tag="ACL8CL_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HGS1331212.1"
                     /translation="MDEKGLKIFLAVLQSIIVILLVYFLSFVRETELERSSMVILYLL
                     HFFVFYFSSYGNNFFKRGYLVEFNSTIRYIFFFAIAISVLNFFIAERFSISRRGMVYF
                     LTLEGISLYLLNFLVKKYWKHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWKL
                     VAVSVLDKSDFQHDKIPVIEKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFEKMG
                     MDVTVNLKAFDKNLGRNKQIHEMVGLNVVTFSTNFYKTSHVISKRILDICGATIGLIL
                     FAIVSLVLVPLIRKDGGPAIFAQTRIGKNGRHFTFYKFRSMQIDAEAIKEQLMDQNTM
                     QGG"
BASE COUNT         1560 a          743 c          998 g         1538 t
ORIGIN      
        1 cacattgtgt actatagtag attgaaacta gaatagtaca cctctgcttc taaaatattg
       61 ttagaaatcg atttgactgt cctgatcgat ttgtcatgtt cttatttcat tttactatat
      121 ttttggttcg cgggaagtct actaagatac ttaaagatgc agatagtgaa aataaaggtg
      181 tagacattac cgtaaaaaag tgatataatc gtatgatgtt caatgtatag gtgttaatca
      241 tgagtagacg ttttaaaaaa tcaggttcac agaaagtgaa gcgaagtgtt aatatagttt
      301 tgttgactat ttatttattg ttagtttgtt ttttattgtt cttaatcttt aagtacaata
      361 tccttgcttt tagatatctt aatctagtgg taactgcgtt agtcctacta gttgccttgg
      421 tagggctact cttgattatc tataaaaaag ctgaaaaatt tactattttt ctgttggtgt
      481 tctctatcct tgtcagctct gtgtcgctct ttgcagtaca gcagtttgtt ggactgacca
      541 atcgtttaaa tgcgacttct aattactcag aatattcaat cagtgtcgct gttttagcag
      601 atagtgagat cgaaaatgtt acgcaactga cgagtgtggc agcaccgact gggactgata
      661 atgaaaatat tcaaaaacta ctagctgata tcaagtcaag tcagaatacc gatttgacgg
      721 tcaaccagag ttcgtcttac ttggcagctt acaagagttt gattgcaggg gagactaagg
      781 ccattgtcct aaatagtgtc tttgaaaata tcatcgagtc agagtatcca gactacgcat
      841 cgaagataaa gaagatttat accaagggat tcactaaaaa agtagaagct cctaagacgt
      901 ctaagaatca gtctttcaat atctatgtta gtggaattga cacctatggt cctattagtt
      961 cggtgtcgcg atcagatgtc aatatcctga tgactgtcaa tcgagatacc aagaaaatcc
     1021 tcttgaccac aacaccacgt gatgcctatg taccaatcgc agatggtgga aataatcaaa
     1081 aagataaatt aacccatgcg ggcatttatg gagttgattc gtccattcac accttagaaa
     1141 atctctatgg agtggatatc aattactatg tgcgattgaa cttcacttct ttcttgaaaa
     1201 tgattgactt attgggaggg atagatgttc ataatgatca agagttttca gctctacatg
     1261 ggaagttcca tttcccagta gggaatgtcc atctagactc tgagcaagct ttaggttttg
     1321 ttcgtgagcg ctactcccta gcagatggcg atcgtgaccg tggtcgcaac caacaaaagg
     1381 tgattgtggc tatccttcaa aaattaacgt caaccgaagc actgaaaaat tatagtacga
     1441 tcattgatag cttgcaagat tctatccaaa caaatatgcc acttgagacc atgataaatt
     1501 tggtcaatgc tcagttagaa agtggtggaa cttacaaagt gaattcgcaa gacttgaaag
     1561 gtacaggtcg gatggatctt ccttcttatg caatgccaga cagtaacctc tatgtgatgg
     1621 aaatagatga tagtagttta gctgtagtta aagcagctat acaggatgtg atggagggta
     1681 gatgaaatga tagacatcca ttcgcatatc gtttttgatg tagatgacgg tcccaaatca
     1741 agagaggaaa gcaaggctct cttggcagaa gcctacagac agggggtgcg aaccattgtc
     1801 tctacctctc accgtcgcaa gggcatgttt gaaactccgg aagagaagat agcagaaaac
     1861 tttcttcagg ttcgggaaat tgcaaaagaa gtagcagatg atttagtcat tgcttatggc
     1921 gcagagatat actatactct ggatgctcta gaaaagctag aaaaaaaaga aattcctacc
     1981 cttaatgata gtcgttatgc cttgattgag tttagcatgc atacttccta tcgtcagatt
     2041 catacgggat tgagcaatat tttgatgttg ggaatcacgc cagtaattgc tcatattgaa
     2101 cgttataatg ctttagagaa taacgaaaaa cgtgttcgtg aactgattga tatggggtgc
     2161 tatactcaga taaatagtta tcatgtttca aaacctaagt tctttggtga aaaatataaa
     2221 ttcatgaaaa agagagctcg gtattttttg gaacgtgatt tagttcatgt agttgcaagt
     2281 gacatgcaca atttagacag tagacctcca tatatgcaac aggcatatga tatcattgct
     2341 aagaaatatg gagcgaaaaa agcgaaagaa ctttttgtag ataatcccag aaaaattata
     2401 atggatcaat taatttagga gaaaatatga aggaacaaaa cactttggaa atcgatgtat
     2461 tgcaactatt cagagtttta tggaaaagga agttggtcat tttattagtg gcaattataa
     2521 cttcttcagt tgcttttgcc tacagtactt ttgttatcaa acctgagttt actagtacga
     2581 ctcggattta tgtagttaac cgtaatcagg gagagaagtc tggtttaacc agtcaagact
     2641 tgcaggcagg atcgtacttg gttaaagact atcgtgaaat tatcctatcg caggatgttt
     2701 tggaggaagt tgtttctgat ttgaaactag atttgacgcc aaaaggtttg gctaataaaa
     2761 tcaaagtaac agtaccagct gatacccgta ttgtctctgt ttcagttaat gatcgagttc
     2821 ctgaagaggc aagccgtatc gctaactctt tgagagaagt agctgctaaa aaaattatca
     2881 gtattactcg tgtttctgat gtgacaacac tggaggaggc aagaccggcg atatcaccgt
     2941 cttcgccaaa tcttaaacgc aatacactaa ttggtttttt ggcaggggtg attggaacta
     3001 gtgttatagt tcttcttctt gaacttttgg acactcgtgt gaaacgtccg gaagatatcg
     3061 aagatacact acagatgaca cttttgggag ttgtaccaaa cttgaataag ttgaaatagg
     3121 agagagagat gccgacatta gaaatagcac aaaaaaaact ggagttcatt aagaaggcag
     3181 aagaatatta caatgccttg tgtacaaata tacagttgag cggagataaa ctaaaagtaa
     3241 tttccgttac ttctgttagc ccgggggaag gaaaaacaac tacttccgta aatatagcat
     3301 ggtcgtttgc gcgtgcaggc tataaaactc ttttgatcga tggcgatatt cgaaattcag
     3361 tcatatcagg attttttaaa tctcgtgaaa aaattacagg gctaacagaa tttttatctg
     3421 ggacagctga tttatctcac ggtttatgtg atacaaatat tgaaaattta tttgtagttc
     3481 aatcgggctc tgtatcacca aaccctacag ccttgttaca aagtaaaaat tttaatgata
     3541 tgattgaaac attgcgtaaa tattttgatt atatcattgt tgatacagca cctattggaa
     3601 ttgttattga tgcggcaatt atcactcaaa agtgtgatgc gtccatcttg gtaacagcaa
     3661 caggtgaggc gaataaacgt gatgtccaaa aagcgcaaca acaattaaaa caaacaggga
     3721 aactgttcct aggagttgtt tttaataaat tagatatctc ggttgataag tatggagttt
     3781 acggtttcta tggaaattat ggtaaaaaat aacttaggaa agattttatg gatgaaaaag
     3841 gattgaaaat ttttctggca gtattacaga gtattattgt cattttattg gtttattttc
     3901 ttagctttgt tagagagaca gaacttgaac gttcttcgat ggttatacta taccttctcc
     3961 acttttttgt attctatttt agttcctatg gtaacaattt ttttaaaaga gggtacctag
     4021 ttgagtttaa tagtactata agatatattt ttttctttgc aatagctata agtgtattaa
     4081 acttttttat agcggaacgg tttagcatct ctagaagagg aatggtatac ttcttaactt
     4141 tagaaggaat atccttatac ttgttaaatt tcttagtaaa gaaatattgg aagcatgtgt
     4201 tttttaatct aaaaaatagc aagaaaattt tactgttaac agtaacgaaa aatatggaaa
     4261 aagttcttga taaattgcta gaatctgatg aactttcatg gaaattggta gcagtaagtg
     4321 ttttggataa atctgatttt caacatgata aaatacctgt aattgaaaag gaaaaaatta
     4381 ttgaatttgc aacgcatgaa gttgtggatg aggtgtttgt caatcttcca ggagagagct
     4441 acgatattgg agaaattatc tctaggtttg agaaaatggg gatggatgta actgtaaatc
     4501 ttaaagcatt tgataagaat ttgggtcgca ataaacaaat tcatgagatg gtaggattga
     4561 atgtagtcac tttctctaca aatttttata aaactagtca tgtgatttca aagagaattc
     4621 tcgatatttg tggtgccact attggcctta ttctttttgc tatagttagt ctagttttag
     4681 ttccattgat tcgtaaagat ggcggaccag ctatttttgc tcaaactcgt atagggaaaa
     4741 atggtcgaca ttttaccttt tataaattcc gttcgatgca gatcgatgct gaagctatca
     4801 aagaacagtt gatggatcaa aatacgatgc aaggtggta
//
LOCUS       DBEROU010000077         4774 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.077, whole genome shotgun sequence.
ACCESSION   DBEROU010000077 DBEROU010000000
VERSION     DBEROU010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.077"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            41..1867
                     /gene="glpO"
                     /locus_tag="ACL8CL_002046"
     CDS             41..1867
                     /gene="glpO"
                     /locus_tag="ACL8CL_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HGS1331213.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKSDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKDQVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITGVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            1937..2641
                     /locus_tag="ACL8CL_002047"
     CDS             1937..2641
                     /locus_tag="ACL8CL_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HGS1331214.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTVGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPIPNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            2938..3141
                     /locus_tag="ACL8CL_002048"
     CDS             2938..3141
                     /locus_tag="ACL8CL_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331215.1"
                     /translation="MPKWSNPDYVNELDPKIVDMLVEFHKSQGTLETPEAQAEIAQKR
                     EEIEQRRAELEDKKQELLNRLNK"
     gene            complement(3228..3836)
                     /locus_tag="ACL8CL_002049"
     CDS             complement(3228..3836)
                     /locus_tag="ACL8CL_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331216.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAIIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            3968..4159
                     /locus_tag="ACL8CL_002050"
     CDS             3968..4159
                     /locus_tag="ACL8CL_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331217.1"
                     /translation="MLNAKIFYIFLCLIVIETTGLLLLKRGLEMGYLDTEAIILAVFS
                     FAFYNLCSFAWVCSTIKNK"
BASE COUNT         1446 a          947 c         1049 g         1332 t
ORIGIN      
        1 actggcagaa taaagcgatt tagttagaaa gtgtgtaaat atggaatttt caaagaaaac
       61 acgtgaattg tcaattaaaa aaatgcagga acgcaccctg gacctcttga ttatcggtgg
      121 aggaatcaca ggagctggtg tagccttgca ggcggcagct agcggtcttg agactggttt
      181 gattgaaatg caagactttg cagaaggaac atctagtcgt tcaacaaaat tggttcacgg
      241 aggacttcgt tacctcaaac aatttgacgt agaagtggtc tcagatacgg tttctgaacg
      301 tgcagtggtt caacaaatcg ctccacacat tccaaaatca gatccaatgc tcttaccagt
      361 ttacgatgaa gatggagcaa cctttagcct cttccgtctt aaagtagcca tggacttgta
      421 cgacctttta gcaggtgtga gcaacacacc aactgcgaac aaggttttga gcaaggatca
      481 agtcttggaa cgccagccaa acttgaagaa ggaaggcttg gtaggaggtg gagtgtatct
      541 tgacttccgt aacaacgatg cgcgtctcgt gattgaaaac atcaaacgtg ccaaccaaga
      601 cggtgccctc attgccaacc acgtgaaggc agaaggcttc ctctttgacg aaagtggcaa
      661 gattacaggt gttgtagctc gtgatctctt gacagaccaa gtatttgaaa tcaaggcccg
      721 tctggttatt aatacaacag gtccttggag tgataaagta cgtaatttgt ctaataaggg
      781 aacgcaattc tcacaaatgc gcccaactaa gggagttcac ttggtagtgg attcaagcaa
      841 aatcaaggtt tcacagccag tttactttga cacaggtttg ggtgacggtc gtatggtctt
      901 tgttctccca cgtgaaaaca agacttactt tggtacaact gatacagact acacaggtga
      961 tttggagcat ccaaaagtaa ctcaagaaga tgtagattat ctacttggca ttgtcaacaa
     1021 ccgctttcca gaatccaaca tcaccattga tgatatcgaa agcagctggg caggtcttcg
     1081 tccattgatt gcagggaaca gtgcctctga ctataatggt ggaaataacg gtaccatcag
     1141 tgatgaaagc tttgacaact tgattgcgac tgttgaatct tatctctcca aagaaaaaac
     1201 acgtgaagat gttgagtctg ctgtcagcaa gcttgaaagt agcacatctg agaaatattt
     1261 ggatccatct gcagtttctc gtgggtctag cttggaccgt gatgacaatg gtcttttgac
     1321 tcttgctggc ggtaaaatca cagactaccg taagatggct gaaggagcta tggagcgcgt
     1381 ggttgacatc ctcaaagcag aatttgaccg tagctttaaa ttgatcaatt ctaaaactta
     1441 ccctgtttca ggtggagaat tgaacccagc aaatgtggat tcagaaatcg aagcctttgc
     1501 gcaacttgga gtatcacgtg gtttggatag caaggaagct cactatctgg caaatcttta
     1561 cggttcaaat gcaccgaaag tctttgcact tgctcacagc ttggaacaag cgccaggact
     1621 cagcttggca gataccttgt cccttcacta tgcaatgcgc aacgagttgg ctcttagccc
     1681 agttgacttc cttcttcgtc gtaccaacca tatgctcttt atgcgtgata gcttggatag
     1741 catcgttgag ccagttttgg atgaaatggg acgattctat gactggacag aagaagaaaa
     1801 agcaacttac cgtgctgatg tcgaagcagc tctcgctaac aacgatttag cagaattaaa
     1861 aaattaagaa aaaataaaag aggtggaggg cagcattcct tgtcgcccgt cccttctttt
     1921 taatggagac agaaagatga tgaatgaatt atttggagaa tttctaggga ctttaatcct
     1981 gattcttcta ggaaatggtg ttgttgcagg tgtggttctt cctaaaacca agagcaatag
     2041 ctcaggttgg attgtgatta ctatgggttg ggggattgca gttgcggttg cagtctttgt
     2101 atctggcaag ctcagtccag ctcatttaaa tccagctgtg acagttggtg tagctttaaa
     2161 aggtgacttg ccttgggctt ccgttttgcc ttatatctta gcccagttcg caggggccat
     2221 gctgggtcag attttggttt ggttgcaatt caaacctcac tatgaggcag aagaaaatgc
     2281 aggcaatatc ctggcaacct tcagtactgg accagccatc aaggatactg tatcaaactt
     2341 gattagcgaa atccttggaa cctttgtttt ggtgttgaca atctttgctt tgggtcttta
     2401 cgattttcag gcaggtatcg gaacctttgc agtgggaact ttgattgtcg gtatcggtct
     2461 atcactaggt gggacaacag gttatgcctt gaacccagct cgtgaccttg gacctcgtat
     2521 catgcacagc atcttgccaa ttccaaacaa gggagacgga gactggtctt acgcttggat
     2581 tcctgttgta ggccctgtta tcggagcagc cttggcagtg cttgtattct cacttttcta
     2641 gtttatactc ttcgaaaatc aaattcaaac cacgtcagcg tcgccttacc gtactcaagt
     2701 acagcttgcg gctagcttcc tagtttgctc tttgattttc attgagtatt agaaaacaat
     2761 tattttgata gagcttgggc aagagcccaa tttcagcaaa aaatgaagta aatcttctca
     2821 taataaaacg catcatatca agcacgaaaa ttccacgagg tcaactacag tcagaaagct
     2881 gaacaacaag ccaaaacgcc caaaaaaggc ggcaaaaagc aagcacctgc aagcaacgtg
     2941 ccgaaatggt caaatcctga ttatgtcaac gaattagacc caaaaatcgt tgatatgcta
     3001 gtagaatttc acaagtcaca aggcactttg gaaactcccg aggcgcaagc agaaatcgcc
     3061 caaaaacgtg aagaaatcga gcaaaggaga gctgagcttg aggataaaaa acaagagctt
     3121 ttgaaccgct tgaacaaata gagtttcgca agtattatgc ttacaaatta cttgagcaat
     3181 taactaaaat ataaaccctg cctttatatc taggcagggt ttatatttta gaaattcacg
     3241 taggttgtta cggtttttac atacccagta tagtttgagt ttctatagta ttcagtgata
     3301 aacttccatt ttctttgagc aacatggata taagtacttg ttatgtagta tggatatggg
     3361 ctttgtgaat ccaagtaaga ctgataagct tgtataccaa aatatgctcc accaattatt
     3421 gcaccccatg gaccccccaa taaagcacct atcctaccaa tcatataact gattccagca
     3481 ccagtcatga agttagcgaa tgtgttagct tgtttattcc catgtattgt gttgacgtaa
     3541 ttccaaacat taggatcgta tgatctaaaa gatatattta ggtcgatttc attcttttga
     3601 taagccatat aaaatgcccc attgatatag acgccgtcag cacgtcgttc aatagtgtct
     3661 acacttccat ctggattgac aacctcaaga acttcatcgc ttaaaatatt tacttgcgta
     3721 tctccgaacc gcactgatga gccattctca aactgagcct caccagatac aactttagag
     3781 tttgccgata agctatcatc agcaaaaaca acaagcgacg gggaaatgct agacatacag
     3841 aaaacagaca taactagcaa acacatgcat ttaaacatct tagacataac ggaaactctt
     3901 ttgtattttt gatttttttc aacttttatt atacaataaa accaaataaa aagaaagcgg
     3961 taacaatatg cttaatgcga aaatttttta tatattttta tgtttgatcg ttatcgaaac
     4021 tacaggcttg ttgttgttga aaagaggtct cgaaatgggt tatttagaca cagaagctat
     4081 tatcctcgca gttttttcat ttgcttttta caacctatgt tcattcgctt gggtctgctc
     4141 tacaataaaa aataaataga cgtattttca aaaaaaaaat gcatatttat attagcaaaa
     4201 tgacgattta aatcgtcgtt tttttgtagt acgacgggca tgtcgtatat ctgaggtgta
     4261 agtcctcggt gggcacctgc taccggtgaa cccaatagcg attcccaagc ctgactatcg
     4321 tgaggtagca gggagaggaa gggatagcga aatcgtggct ctacgaacag gaacgtgata
     4381 gtaaggcgta tatagcggat aaggaggctt caaactctaa agtccaaaaa ggtagtcgta
     4441 acctatatgt gtaaatcacg agagtaattg aattcggact aaggtttgtg tgaaaaagat
     4501 aaatctttct agagtctaaa gactctgcgt cagatttcct attttcactg taacctttta
     4561 acgtcctcat atcttgtata aacgaggaaa gatgtacgac ttatcccgtg aggtttcatg
     4621 agcgctgaaa gcgtagtaac aacgaatcat gagaagtcag ccgagcccat agtagtgagg
     4681 aaacttctgt agtggaagtg gagcgaaggg gtgaatactc aaacagtctg gggagagact
     4741 gtttgaggtc tgtcgctaga aagagaaaac gaca
//
LOCUS       DBEROU010000078         4679 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.078, whole genome shotgun sequence.
ACCESSION   DBEROU010000078 DBEROU010000000
VERSION     DBEROU010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.078"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..370
                     /locus_tag="ACL8CL_002051"
     CDS             <1..370
                     /locus_tag="ACL8CL_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HGS1331218.1"
                     /translation="GAMATGWAKVNGSWYYLNANGSMATGWLQYNGSWYYLNANGAMA
                     TGWAKVNGSWYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLG
                     ALAVNTTVDGYKVNANGEWV"
     gene            771..1892
                     /gene="mnmA"
                     /locus_tag="ACL8CL_002052"
     CDS             771..1892
                     /gene="mnmA"
                     /locus_tag="ACL8CL_002052"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HGS1331219.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAITLGADYVATGHYARVARDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKALSKNILYVGQ
                     GFYHDSLMSTSLEASQVHFTCDMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
     gene            2033..2488
                     /locus_tag="ACL8CL_002053"
     CDS             2033..2488
                     /locus_tag="ACL8CL_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HGS1331220.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEDAAVREVKEELGVKAQAGQLAFVVENRFEVDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            2498..4411
                     /gene="mnmG"
                     /locus_tag="ACL8CL_002054"
     CDS             2498..4411
                     /gene="mnmG"
                     /locus_tag="ACL8CL_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HGS1331221.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMIDEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEVPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEDLDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
BASE COUNT         1344 a          952 c         1153 g         1230 t
ORIGIN      
        1 cggcgctatg gcaacaggtt gggctaaagt caacggttca tggtactacc tcaacgctaa
       61 cggttcaatg gcaacaggtt ggctccaata caacggttca tggtactacc tcaacgctaa
      121 cggtgctatg gcaacaggtt gggctaaagt caacggttca tggtactacc taaacgctaa
      181 cggttcaatg gcaacaggtt gggtgaaaga tggagatacc tggtactatc ttgaagcatc
      241 aggtgctatg aaagcaagcc aatggttcaa agtatcagat aaatggtact atgtcaatgg
      301 cttaggtgcc cttgcagtca acacaactgt agatggctat aaagtcaatg ccaatggtga
      361 atgggtttaa gccgattaaa ttaaatcatg ttaagaacat ttgacatttt aattttgaaa
      421 caaagataag gttcgattga atagatttat gttcgtattc tttaggtacc tatcttatga
      481 tttcaggaaa tgtcattaaa aaaacgactc cttttctcta acttgaaaaa tagattagag
      541 aaaatgggtt gttttattta ttatagttat ttgaatgaag ataagaataa ggtatactca
      601 catcatttac ataatctgta tattgactat aagttttaaa aaacaatttt taagctcttc
      661 cttgtcttct ctaaccaagc gtgttataat gaatactgct caagcgacct tcaatcgtga
      721 agcacacacg accttcaatc gtgaataaac gaatagatgg gagacttacc atgagtgata
      781 actctaaaac acgtgttgtc gtggggatga gtggtggtgt tgattcgtcg gtgacggctc
      841 ttttgctcaa ggagcagggc tacgatgtga tcggtatctt catgaagaac tgggatgaca
      901 cagatgaaaa cggcgtctgt acggcgaccg aagattacaa ggatgtggtt gcggtggcag
      961 accagattgg cattccctac tactctgtca attttgaaaa agagtactgg gaccgcgttt
     1021 ttgagtattt cctagcggaa taccgtgcag ggcgcacgcc aaatccggac gttatgtgca
     1081 acaaggaaat caagttcaag gcctttttgg actatgccat aaccttgggg gcagactatg
     1141 tagcgactgg gcattatgct cgagtggcgc gtgatgagga tggtaccgtt cacatgcttc
     1201 gtggcgtgga caatggcaag gatcagacct atttcctcag ccaactttcg caagaacaac
     1261 ttcaaaaaac catgttccca ctaggacatt tggaaaagcc tgaagttcga aaactagcag
     1321 aagaagcagg tctttcgact gctaagaaga aagactcgac agggatttgc tttatcggag
     1381 aaaagaactt taaaaacttt ctcagcaact acctgccagc tcagcctggt cgcatgatga
     1441 ctgtggatgg tcgcgatatg ggcgagcatg caggtctgat gtactatacg attggtcagc
     1501 gtggcgggct tggtatcggt gggcaacacg gtggtgacaa tgccccttgg ttcgttgtcg
     1561 gaaaagctct aagcaagaat attctctatg taggacaagg attctaccat gattcgctca
     1621 tgtcaaccag cctagaggct agccaagtcc actttacttg tgatatgccc gaggagttta
     1681 cgctagaatg tacggctaaa ttccgttacc gtcagcctga ctctaaggtg accgtccatg
     1741 tcaaaggaga caaggcagag gtcatctttg cggaaccaca acgcgccatt acaccaggac
     1801 aggcagttgt cttttacgat ggcgaagagt gtctaggtgg cggtttgatt gacaatgctt
     1861 accgcgatgg acaagtttgt cagtacattt agattgacaa attttctcaa tttgctacaa
     1921 taataacagc aatagaaatg atggtcaaag ctcatggatg ttgcaggctt ttttgtcctg
     1981 cacttctttg tagttttgac tgtttttgtg tcgtttaagg gaaaggacaa gaatgactca
     2041 acaagacttt cggacaaaag tagacaatac ggtttttggt gttcgggcga cagccttgat
     2101 tgtccaaaat cacaagcttc tagttaccaa agacaagggc aagtattaca ctattggagg
     2161 tgcgattcaa gtcaatgaaa gcacggaaga cgcggcagtc cgtgaagtga aggaagaact
     2221 gggtgtcaaa gctcaagctg ggcagctagc ttttgtggtt gaaaatcgtt ttgaagtgga
     2281 cggcgtttcc tatcacaata tcgagtttca ttatctggtg gatttgcttg aagatgcccc
     2341 gttgaccatg caggaagatg agaaaaggca gccctgtgag tggattgact tggataagct
     2401 tgaggatatc cagctagttc cagccttttt aaaaacggcc ctaccagatt gggaaggcca
     2461 actaagacac attcatcttg aggaatagga gagaaacatg acttatcatt ttactgaaga
     2521 atacgatatt attgtaattg gtgcgggaca cgctggggtt gaggcttcct tggccgctag
     2581 ccgtatgggc tgtaaggtcc tgcttgcgac catcaatatt gaaatgctgg ctttcatgcc
     2641 ttgtaatccc tctatcggtg gttctgccaa ggggattgtc gtgcgtgaag tcgatgccct
     2701 cggtggcgag atggccaaaa ccattgacaa gacttacatc cagatgaaga tgctaaacac
     2761 agggaagggg ccagctgtcc gtgcccttcg tgcgcaggct gacaaggaac tttactctaa
     2821 ggagatgcgc aagacggttg aaaaccaaga aaatctgacc cttcgtcaaa ccatgattga
     2881 tgagattttg gtggaagatg gcaaggttgt cggtgtgcgt acagccaccc atcaagaata
     2941 tgctgctaag gctgttattg tgacgacagg gactgctctc cgtggggaaa ttatcatcgg
     3001 agacctcaag tactcatcag gtcctaacca cagcttggct tctattaacc tagctgataa
     3061 tctcaaggaa ttaggactcg aaatcggtcg tttcaagaca ggaacccctc cacgtgtcaa
     3121 ggcttcttct atcaactacg acgtgacaga aattcagcca ggagacgaag tgcctaatca
     3181 tttctcatac acttcacgtg atgaggatta tgtcaaagat caagtgccat gctggttgac
     3241 ctacaccaat ggtaccagtc atgagattat ccaaaacaac ctccaccgtg cgcctatgtt
     3301 tacaggtgtg gtcaagggag tggggcctcg ttactgtccg tcgattgaag acaagattgt
     3361 gcgctttgcg gacaaggaac gtcaccaact cttccttgag ccagaagggc gcaatactga
     3421 ggaagtctat gttcaaggac tttcaaccag tctgcctgag gatgtccagc gtgacttggt
     3481 tcattccatc aaaggtttgg aaaatgcaga gatgatgcgg acaggttatg ctattgagta
     3541 tgatatggtc ttgcctcatc agttgcgtgc gactctggaa accaagaaaa tctcaggtct
     3601 cttcactgct ggtcagacaa atggaacgtc aggttacgaa gaggcagcag gccaagggat
     3661 tatcgcgggt atcaatgcgg ctctgaaaat ccaaggtaag cctgagttga ttttgaagcg
     3721 aagtgacggt tatatcgggg tcatgatcga cgacttggtg accaagggaa ccattgaacc
     3781 ttaccgtctc ttgaccagtc gtgctgaata tcgtctcatt cttcgccatg acaatgctga
     3841 tatgcgcttg actgagatgg gacgcgagat tggccttgtg gatgatgaac gctgggctcg
     3901 ttttgaaatc aagaaaaatc aatttgataa tgagatgaaa cgcctagaca gtatcaaact
     3961 caagccagtc aaggaaacca atgctaaggt tgaggaaatg ggcttcaagc cgttgacaga
     4021 tgcggtgaca gccaaggaat tccttcgccg tccagaagtt tcttaccaag atgtggtggc
     4081 cttcatcgga ccagctgcag aagacttgga tgacaagatt atcgaattga ttgaaacaga
     4141 aatcaagtat gaaggctata tttccaaagc catggaccag gttgccaaga tgaaacgcat
     4201 ggaagaaaaa cgcattccgg ccaatatcga ctgggatgac attgattcta tcgcaaccga
     4261 agcccgtcag aagttcaaac tcatcaatcc agaaaccatc ggccaagcca gccgtatttc
     4321 gggagtaaac ccagcagata tttctatttt gatggtgtat ctggaaggta aaaatcgtag
     4381 tatttctaaa actcttcaaa aatcaaaatg atacgtcgta ggcttcttac gaatgagttc
     4441 aaagcttggc tttgattcat ctccaacctc ccatagttcc ccgaactatg ggagctaact
     4501 caagttatgc attttcctag ccttctggtc tgattttcat tgagttttaa tctaaaaaga
     4561 aagcatagag aaaaacagct ccgaagacgg ggctgttttg ttgatttgta ctcaatgaaa
     4621 atcaaagagc aaactaggaa gctagccgta ggttgctcaa agcactgctt tgaggttgt
//
LOCUS       DBEROU010000079         4555 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.079, whole genome shotgun sequence.
ACCESSION   DBEROU010000079 DBEROU010000000
VERSION     DBEROU010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4555
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.079"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            453..1799
                     /gene="gdhA"
                     /locus_tag="ACL8CL_002055"
     CDS             453..1799
                     /gene="gdhA"
                     /locus_tag="ACL8CL_002055"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HGS1331222.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVVSFRVPWVDRDGKIQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHGGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVDQGVICVSEGANMPSDLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGIV"
     gene            complement(2305..2475)
                     /locus_tag="ACL8CL_002056"
     CDS             complement(2305..2475)
                     /locus_tag="ACL8CL_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331223.1"
                     /translation="MKKENEYVILTIASLGVMIGIVFAIFLDFPVEYGISLGLLNGIV
                     LGSLIVYKNNKN"
     gene            complement(2488..3060)
                     /locus_tag="ACL8CL_002057"
                     /pseudo
     CDS             complement(2488..3060)
                     /locus_tag="ACL8CL_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082046.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            complement(3083..3888)
                     /locus_tag="ACL8CL_002058"
                     /pseudo
     CDS             complement(3083..3888)
                     /locus_tag="ACL8CL_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            4144..4555
                     /locus_tag="ACL8CL_002059"
                     /pseudo
     CDS             4144..4555
                     /locus_tag="ACL8CL_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1412 a          844 c          775 g         1524 t
ORIGIN      
        1 ttcttgtttc attttactat agaacgattt gaaggcgttt atgatattta gctgtactag
       61 agttttttaa aagtgttttg atggtttgga tttcttcttt aattgatttc atattactat
      121 tatataatgc tttttgattt tagtctggta taaatattgc tttcctccaa aatggtcata
      181 gttttactgg caaatctaac atatcacgga taaattaaca agtgatttct gaattactaa
      241 acattttctt ttcttatagc ataccatact ttaagatttt gtctttaaga aagatatttc
      301 caagaaaaac gttcgttttt ttatctattt tcaaaaaaac caaaaactga attgaaagaa
      361 tttttagaaa atttctgttt tttctttaca gtgaaaaaaa attctgctat aatgtcttat
      421 gtaataaaat atgacaataa aaggagaacg atatgacatc tgctaaagaa tatatccaaa
      481 gcgtgtttga aactgtgaaa gctcgtaacg ggcacgaggc tgaattcctc caagctgttg
      541 aagaattttt caacacttta gaacctgtat ttgaaaaaca ccctgagtat atcgaagaaa
      601 atatcttggc acgtattact gagcctgagc gcgtggtttc tttccgtgtt ccttgggttg
      661 accgtgatgg aaaaattcaa gtaaaccgtg gttaccgtgt tcaattcaac tcagctgttg
      721 gaccatacaa aggtggactt cgtttccacc caactgtaaa ccaagggatc ttgaaattcc
      781 tcggatttga acaaatcttt aaaaatgtct tgactggact tcctatcggt ggaggtaaag
      841 gtggatcaga cttcgatcct aaaggtaaaa cagatgctga agtgatgcgc ttctgccaaa
      901 gcttcatgac tgaattgcaa aaacacatcg gaccatcact tgacgtacct gctggtgata
      961 tcggtgttgg tggacgtgag attggttacc tttacggtca atacaaacgt cttaaccaat
     1021 ttgatgctgg tgtcttgact ggtaaacctc ttggatttgg tggtagcttg attcgtccag
     1081 aagcaactgg ttacggtttg gtttactata ctgaagaaat gctcaaagct aacggtaaca
     1141 gctttgctgg taagaaagtc gttatttcag gttctggtaa cgttgctcaa tatgctcttc
     1201 aaaaagcaac tgaactcggt gcaactgtta tttctgtttc tgactcaaat ggttatgtca
     1261 tcgatgaaaa tggtatcgac ttcgatcttt tggttgatgt taaagaaaaa cgtcgtgctc
     1321 gtttgactga gtatgcagct gaaaaagcaa ctgctactta tcatggaggt tctgtatgga
     1381 cttacgctgg taactatgat atcgctcttc catgtgcgac tcaaaacgaa atcaacggtg
     1441 aagcagctaa acgtttggtt gatcaaggcg ttatctgtgt atctgaaggt gccaacatgc
     1501 cgagcgacct tgatgccatc aaagtttaca aagaaaatgg tatcttctac ggacctgcca
     1561 aagctgccaa cgctggtggt gtagccgttt cagcccttga aatgagccaa aatagtcttc
     1621 gcctctcatg gactcgtgaa gaagttgatg gccgtctcaa agacatcatg acaaacatct
     1681 tcaacacagc taaaacgact tcagaaacat acggtcttga taaagactac cttgcaggag
     1741 caaacattgc tgcctttgaa aatgtagcaa atgccatgat tgcacaaggt attgtttaag
     1801 atttatttac tcaatcctca atcactacga ttggggattt ttagcttgaa ttaaaaaatt
     1861 ggaaaatcta attaaataaa atgaaatgaa ataaaaactg aacaaattga ttgcggaatt
     1921 caaaccaatt tctaacatac ttctagaaat taatttgact tttctaatcg atttgttcat
     1981 atcttatttc aatttactat acttacttaa ccctttttat actcaatgaa aatcaaagag
     2041 caaactagga agcgagccgc aggctgtact tgagtacggc aaggcaaagc tgacgtggtt
     2101 tgaagagatt ttcaaagagt attaaacaaa aagaaaaacc gctactccta agaatagcaa
     2161 tttaatcatg tttttaagct actactgtag ttgctctatt atttaagtgt tatgaataac
     2221 ttcaatatct ttaaagcttt ttgttagatt tataatttca actatagcca tctctactcc
     2281 ttagtttaca gcaaaaaatt atgcttaatt cttattgttt ttgtaaacaa tcagcgaacc
     2341 caatactatt ccattcaaca agcctaaaga aataccatat tcaactggaa aatctaaaaa
     2401 aattgcaaac actattccaa tcatcacccc tagtgaggct attgttaaaa ttacatattc
     2461 attttctttc ttcattttct ttacacctca tttccttttt gtatataagc tgtagtactc
     2521 tccaccaaga gttattaagt acttatgatt tcctgactca acaatcttac cacctttcat
     2581 tacaaaaata atatccgcat ccttaatcgt tgacaatcta tgagctataa ttatttgagt
     2641 acaaccctga ctttgtatat actttgttat tctttcctca ttaatagtgt ctaatgcact
     2701 agttgcttca tctaaaatta caatactagg attatttatt aatgcacgtg ccagtgctat
     2761 ccgttgcctt tgcccacctg aaatatttga ccccatctct gagatgatag tattaaattt
     2821 catcggcata gccatgattt catcatagat ttgaactgct ttacaaactt cctctatctt
     2881 ttgtgaagta acttcgtgct ttaaagttat attatcaaga atacttcggt tcaataaaaa
     2941 tgaatcctgt ggaactactc ctaaattttg acttaatatt tttttatcta tttgattaat
     3001 atttatacca taaaataaaa cttttcctgt atcaatctta tataatcctg atggtatttt
     3061 tgataataga cttcctgcaa aactagaatc ctagttcatg attgataata ccaacaatca
     3121 aattcatccg taatccgaag cgtttacgat gatttcgata ggttgttgaa aacattttaa
     3181 gcgtttttac tttggcaaag atgttctcaa ccttgcttct ctccttagat agcacatggt
     3241 tacaggcttt atcttcagct gttagcggct tgagtttgct ggatttacgt ggagtttgtg
     3301 cttgaagata tatcttcatg agcccttgat aaccactgtc agccaagatt ttaccagctt
     3361 gtccgatatt tctgcgactc attttgaaca acttcatatc atgacaatag ttcacagcga
     3421 tatccaaaga aataattctc ccttgacttg tgacaatcgc ttgagtcttc atagcgtgaa
     3481 atttcttttt accagaataa ttcgctaatt ctttttttag gtcgattgat ttttacttcc
     3541 gtcgcatcaa tcattaccgt gtcctcagaa ctgagaggag ttcttgaaat cgcaacacca
     3601 cctttgaaca agagttactt caacccattg gctccgacgg attaagttgc tttcgtgaat
     3661 accaagatca gccgaaattt cttcataagt tcgatattct cgcacatatt gaagagtggc
     3721 cataagaagg tcttctaggc ttaatttagg ttttcgccca ccttttgcgt gtttaagttg
     3781 ataagctgtt tttaatacag ctaacatctc ttcaaaagtc gtgcgttgaa caccaacaag
     3841 acgcttaaat cgtgcatcgg ttagttgttt acttgcttca taattcatag aactattata
     3901 ccatattttg tttcgcagga agtctattga aaaatctcca gactagagaa ctcacggata
     3961 gttcctaatc tggagatttc ttatttgcac ttttcttgta caactttagt ccacggtaaa
     4021 taggcctcta aaacctcttt gtttacgaga gtttcctcgt ttggaagaca ttctagaaga
     4081 taggatagat atttctcgct atttatacta gactaaaatc aaaaagcatt atacaatagt
     4141 gatatgaaat caactaaaga agaaatccaa accatcaaaa cacttttaaa agactctcgt
     4201 acagctaaat atcataaacg ccttcaaatc gttctatttc gtctgatggg caaatcttat
     4261 aaagagatta tagagctttt agactgtaat taaacaacga tttagcaaaa tgtaaaaaaa
     4321 tatgaggagt tcggactcga ctctctcctt caagaaacac gtggtggtcg taaccatgca
     4381 tatatgacag ttgaggaaga gaaagtcttt cttgcccgtc atttgaaggc tacagaggca
     4441 ggagaatttg ttacaattga tgccttattt caggcttata aaaaggagtt aggtcgttcc
     4501 tacacacgtg atgccttcta tcaactgttg aagcgccatg gttggcgaaa tatta
//
LOCUS       DBEROU010000080         4478 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.080, whole genome shotgun sequence.
ACCESSION   DBEROU010000080 DBEROU010000000
VERSION     DBEROU010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.080"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            163..816
                     /gene="rr06"
                     /locus_tag="ACL8CL_002060"
     CDS             163..816
                     /gene="rr06"
                     /locus_tag="ACL8CL_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HGS1331224.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            813..2153
                     /gene="hk06"
                     /locus_tag="ACL8CL_002061"
     CDS             813..2153
                     /gene="hk06"
                     /locus_tag="ACL8CL_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HGS1331225.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGEVGKQINGMYEHLLKV
                     IHELESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIA
                     KSINKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIEN
                     QLTDATRVVMSLRALDKVLTNLISNAIKYSDKNGRVIISEQDGYLSIKNTCAPLSDQE
                     LEHLFDIFYHSQIVTDKDESSGLGLYIVNNILESYQMDYSFLPYEHGMEFKISL"
     gene            2236..2763
                     /locus_tag="ACL8CL_002062"
     CDS             2236..2763
                     /locus_tag="ACL8CL_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HGS1331226.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     MAHIVFYLSCLVEAMVHKTIFDGMGMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYVTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            2842..>4478
                     /gene="pspC"
                     /locus_tag="ACL8CL_002063"
     CDS             2842..>4478
                     /gene="pspC"
                     /locus_tag="ACL8CL_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     choline-binding form"
                     /protein_id="HGS1331227.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVAVASLFLGGVVHAEGVRSEN
                     TPKVTSSGDEVDEYIKKMLSEIQLDKRKHTHNFALNLKLSRIKTEYLYKLKVNVLEEK
                     SKAELTSKTKKEVDAAFEKFKKDTLKLGEKVAEAQKKVEEAKKKAKDQKEEDHRNYPT
                     NTYKTLELEIAESDVKVKEAELELLKEEAKTRNEDTINQAKAKVKSEQAEATRLKKIK
                     TDREQAEATRLENIKTDREKAEEVKDKLKRRTKRAVPGEPATPDKKENDAKSSDSSVG
                     EETLPSPSLKSGKKVAEAQKKVAEAEKKAKDQKEEDRRNYPTNTYKTLDLEIAESDVK
                     VKEAELELVKEEAKESRNEEKVKQAKAKVESKKAEATRLENIKTDRKKAEEAKRRAAE
                     EDKVKEKPAEQPQPAPAPQPEKPTEEPENPAPAPKPENPAEQPKAEKPADQQAEEDYA
                     RRSEEEYNRLTQQQPPKTEKPAQPSTPKTGWKQENGMWYFYNTDGSMATGWLQNNGSW
                     YYLNSNGAMATGWLQNNGSWYYLNANGSMATGWLQYNG"
BASE COUNT         1608 a          673 c          953 g         1244 t
ORIGIN      
        1 agctgcaaga tgaatgattt tcttgtattg acgttgttgt tgacaaaaag tagcggataa
       61 atgaaatcca ttccattatc atagatgata ggctggtagg aaattttcaa atagcataca
      121 ggaaatagat gtatggagtt ctggtagtag aaagggagag agatgaacat tttagttgca
      181 gatgacgagg aaatgattag agaaggaatt gcagcatttc tgacagaaga gggttatcat
      241 gtcattatgg ctaaggatgg acaagaggtc ttggaaaaat ttcaagatct ccctatccat
      301 ctcatggtac tggatttaat gatgcctagg aagagtggtt ttgaagtgtt aaaagaaatc
      361 aatcaaaagc acgatattcc tgtcatcgtc ttgagtgctc tgggagatga aactactcag
      421 tcacaggtat ttgatctcta tgctgatgat catgtgacaa aacctttttc tttggtactg
      481 cttgtcaagc gtattaaggc gcttatcaga cgttactacg tcatagagga tctttggcga
      541 tatcaggatg taacagtgga ttttacctct tacaaagcac attataaaaa tgaagaaatt
      601 gatctcaaac caaaggaatt actggtacta aagtgtttga ttcagcataa aaatcaagtt
      661 ttaagtagag agcagatatt ggaagaaatt tcaaaagatg tagctgattt accttgtgat
      721 agggtcgttg atgtctatat tcgtactctt cgcaaaaaat tagctttaga ttgtatcgtg
      781 actgtgaaaa atgttgggta taagattagc ttatgataaa aaatcctaaa ttattaacca
      841 agtctttttt aagaagtttt gcaattctag gtggtgttgg tctagtcatt catatagcta
      901 tttatttgac ctttcctttt tattatattc aactggaggg ggaaaagttt aatgagagcg
      961 caagagtgtt tacggagtat ttaaagacta agacatctga tgaaattcca agcttactcc
     1021 agtcttattc aaagtccttg accatatctg ctcaccttaa aagagatatt gtagataagc
     1081 ggctccctct tgtgcatgac ttggatatta aagatggaaa gctatcaaat tatatcgtga
     1141 tgttagatat gtctgttagt acagcagatg gtaaacaggt aaccgtgcaa tttgttcacg
     1201 gggtggatgt ctacaaagaa gcaaagaata ttttgctttt gtatctccca tatacatttt
     1261 tggttacaat tgctttttcc tttgtttttt cttattttta tactaaacgc ttgctcaatc
     1321 ctctttttta catttcagaa gtgactagta aaatgcaaga tttggatgac aatattcgtt
     1381 ttgatgaaag taggaaagat gaagttggtg aagttggtga agttggaaaa cagattaatg
     1441 gtatgtatga gcacttgttg aaggttattc atgagttgga aagtcgtaat gagcaaattg
     1501 taaaattgca aaatcaaaag gtttcctttg tccgcggagc atcacatgag ttgaaaaccc
     1561 ctttagccag tcttagaatt atcctagaga atatgcagca taatattgga gattacaaag
     1621 atcatccaaa atatattgca aagagtataa ataagattga ccagatgagc cacttattgg
     1681 aagaagtact ggagtcttct aaattccaag agtggacaga gtgtcgtgag accttgactg
     1741 ttaagccagt tttagtagat attttatcac gttatcaaga attagctcat tcaataggtg
     1801 ttacaattga aaatcaattg acagatgcta ccagggtcgt catgagtctt agggcattgg
     1861 ataaggtttt gacaaacctg attagtaatg caattaaata ttcagataaa aatgggcgcg
     1921 taatcatatc cgagcaagat ggctatctct ctatcaaaaa tacatgtgcg cctctaagtg
     1981 accaagaact agaacattta tttgatatat tctatcattc tcaaatcgtg acagataagg
     2041 atgaaagttc cggtttgggt ctttacattg tgaataatat tttagaaagc tatcaaatgg
     2101 actatagttt tctcccttat gaacacggta tggaatttaa gattagcttg tagacagatt
     2161 agttttttat taaagttcat atagggttaa cataagtgtg ttattctttg tgtagataaa
     2221 agaaaggata ctaatatggt attagcgatt attttagtaa cattccttat tcgattgatt
     2281 tttttaaagc gttcgataga gaatgagaaa cgaatcctta gcaatggcgg gaaagaattt
     2341 ggagttgaga atacaaaacg attaactatg gctcatattg ttttttatct ctcttgcttg
     2401 gttgaggcaa tggtgcacaa gacaattttt gatggcatgg gcatggttgg tttagtcttg
     2461 cttatttttt ctatgctgat gttgatgttg gtgattcact tgttgggaga tatttggaca
     2521 gtgaagctta tgcttgtcaa taatcacaaa tatgtagatc atatcttgtt taggacagta
     2581 aaacacccta attacttttt aaatatttta cctgagttga ttggcttgac cttgttgagt
     2641 catgcttatg tgacttttgt tttagttttt ccagtttatg cagttatttt gtatcgacga
     2701 atagctgaag aggaaaagct attacatgaa gttataatcc caaatggaag cataaagaga
     2761 taaatacaaa attcgattta tatacagttc atattgaagt gatatagtaa ggttaaagaa
     2821 aaaatataga aggaaataat tatgtttgca tcaaaaagcg aaagaaaagt acattattca
     2881 attcgtaaat ttagtattgg agtagctagt gtagctgttg ctagcttgtt cttaggagga
     2941 gtagtccatg cagaaggggt tagaagtgag aataccccca aggttacatc tagtggggat
     3001 gaagtcgatg aatatataaa aaaaatgttg agtgagatcc aattagataa aagaaaacat
     3061 acccacaatt tcgccttaaa cctaaagttg agcagaatta aaacggagta tttgtataaa
     3121 ttaaaagtta atgttttaga agaaaagtca aaagctgagt tgacgtcaaa aacaaaaaaa
     3181 gaggtagacg cagcttttga gaagtttaaa aaagatacat tgaaactagg agaaaaggta
     3241 gcagaagctc agaagaaggt tgaagaagct aagaaaaaag ccaaggatca aaaagaagaa
     3301 gatcaccgta actacccaac caatacttac aaaacgcttg aacttgaaat tgctgagtcc
     3361 gatgtgaaag ttaaagaagc ggagcttgaa ctattgaaag aggaagctaa aactcgaaac
     3421 gaggacacaa ttaaccaagc aaaagcgaaa gttaagagtg aacaagctga ggctacaagg
     3481 ttaaaaaaaa tcaagacaga tcgtgaacaa gctgaggcta caaggttaga aaacatcaag
     3541 acagatcgtg aaaaagcaga agaagttaaa gataaactaa agaggcggac aaaacgagca
     3601 gttcctggag agccagcaac acctgataaa aaagaaaatg atgcgaagtc ttcagattct
     3661 agcgtaggtg aagaaactct tccaagccca tccctgaaat caggaaaaaa ggtagcagaa
     3721 gctcagaaga aggtagcaga agctgagaaa aaagccaagg atcaaaaaga agaagatcgc
     3781 cgtaactacc caaccaatac ttacaaaacg cttgaccttg aaattgctga gtccgatgtg
     3841 aaagttaaag aagcggagct tgaactagta aaagaggaag ctaaggaatc tcgaaacgag
     3901 gaaaaagtta agcaagcaaa agcgaaagtt gagagtaaaa aagctgaggc tacaaggtta
     3961 gaaaacatca agacagatcg taaaaaagca gaagaagcta aacgaagagc agcagaagaa
     4021 gataaagtta aagaaaaacc agctgaacaa ccacaaccag cgccggctcc tcaaccagaa
     4081 aaaccaactg aagagcctga gaatccagct ccagctccaa aacctgagaa tccagctgaa
     4141 caaccaaaag cagaaaaacc agctgatcaa caagctgaag aagactatgc tcgtagatca
     4201 gaagaagaat ataatcgctt gactcaacag caaccgccaa aaactgaaaa accagcacaa
     4261 ccatctactc caaaaacagg ctggaaacaa gaaaacggta tgtggtactt ctacaatact
     4321 gatggttcaa tggcgacagg atggctccaa aacaatggct catggtacta cctaaacagc
     4381 aatggcgcta tggcgacagg atggctccaa aacaatggtt catggtacta tctaaacgct
     4441 aatggttcaa tggcaacagg atggctccaa tacaatgg
//
LOCUS       DBEROU010000081         4425 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.081, whole genome shotgun sequence.
ACCESSION   DBEROU010000081 DBEROU010000000
VERSION     DBEROU010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.081"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..363)
                     /locus_tag="ACL8CL_002064"
     CDS             complement(<1..363)
                     /locus_tag="ACL8CL_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331228.1"
                     /translation="MRKSGFLIYYVLFCIVCFLLGLLLSFILKNETFATIPPKLELQF
                     TLTVLQANSTNFLLYLFCFPISPLLQLFDLLSLGIQLGISGQMIGIVATIKGILPHAL
                     IEIPNFIWYQGMSQYLLYR"
     gene            complement(360..1492)
                     /locus_tag="ACL8CL_002065"
                     /pseudo
     CDS             complement(360..1492)
                     /locus_tag="ACL8CL_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1499..1795)
                     /locus_tag="ACL8CL_002066"
     CDS             complement(1499..1795)
                     /locus_tag="ACL8CL_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uberolysin/carnocyclin family circular
                     bacteriocin"
                     /protein_id="HGS1331229.1"
                     /translation="MKSKRMEFHNKFLIVSAMLAVISWLSLGVVSFPMLAGTLGISTK
                     AAATVVNLISAYSTVTAVISIVGAITGVGSIGSGIAATVLYILKKKGAAKAALW"
     gene            complement(2326..3219)
                     /locus_tag="ACL8CL_002067"
     CDS             complement(2326..3219)
                     /locus_tag="ACL8CL_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HGS1331230.1"
                     /translation="MDELKREIGYKIKTIRRSCGKTQIDICGDESELTIRQLARIENG
                     QALATLPKLLLIADKLGVSLQNIVDVKVIEIPKGFLKLKDELIHSQTYADKGRIERKE
                     AILEEIYENYYENLPEEEQLIVDVTQARFDIYGSSDVTYGLGLVEEYFQQLLKKKYFS
                     VNDLLIIELYFFCCAMGLEDKEHFEELAQKVLLCSEYEDKASLVQMEKVLLSLFIQIQ
                     TEDSLIYIQTFEKIIAKTRHVFYRPHLFLLKAKYALFVDKNILEAESFYEKAISLAEL
                     LDDQVLVQRILAEKQIDFPTT"
     gene            complement(3240..3434)
                     /locus_tag="ACL8CL_002068"
     CDS             complement(3240..3434)
                     /locus_tag="ACL8CL_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331231.1"
                     /translation="MKKVLKYFLVFVFLFLMNIFIFKILATLGFQLIMSEKSYIVLPL
                     FSLIVLYMIDKRIRKKKKSL"
     gene            complement(3845..3970)
                     /locus_tag="ACL8CL_002069"
     CDS             complement(3845..3970)
                     /locus_tag="ACL8CL_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PncF family bacteriocin immunity protein"
                     /protein_id="HGS1331232.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKKFLKSSEKKTEKSVKK"
     gene            complement(3973..4203)
                     /gene="blpU"
                     /locus_tag="ACL8CL_002070"
     CDS             complement(3973..4203)
                     /gene="blpU"
                     /locus_tag="ACL8CL_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpU"
                     /protein_id="HGS1331233.1"
                     /translation="MNTKTMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAARGLQ
                     LGIKTRTWQGAATGAVGGAILGGVAYAATCWW"
BASE COUNT         1621 a          810 c          505 g         1489 t
ORIGIN      
        1 tcggtataaa agatactgac tcataccttg ataccaaata aaattcggta tttcaataag
       61 agcatgaggt aatattcctt ttattgtagc tactattcca atcatctgac cagatatacc
      121 caattgtatt cctaaagata gcaaatcaaa aagttgcaat aagggcgaaa tcggaaaaca
      181 gaatagatat agcaaaaaat ttgtagaatt tgcctgtaaa actgtcaatg taaattgtaa
      241 ttccagtttc ggaggtatag tagcaaaagt ctcatttttt aatataaagc ttaaaagtaa
      301 acctagtaaa aaacagacta tacaaaataa cacatagtaa atcagaaaac cactttttct
      361 catctaatgc cccctactaa aattacaaat attatcagaa aacaatataa aaccagaaaa
      421 ctccatctca ataaactaac tgtaccttta attaaaaata gagctttctt ataatagaca
      481 taggctataa aataattgaa tggaatcaat aacaaattca acaaaatact ttttatatca
      541 ttactataac ttgtcaccgg aatcaaacat aacaacaatg aaaattgtaa gaaagagatt
      601 aaatgcgtca acatcattag ttgtgcattc ttaaaaaaga aattcatttt agaatgatta
      661 agagcagata attgaagtaa agctagatct accccaattg aggatttatg attccctatt
      721 attgcctcca ttaatgtaat atatattgag gtataaagaa ataatattgg tatttttgtg
      781 tcatcaattg ataacatcgt catcatatac caaaatagat aggaaagaag aatactaatc
      841 aaaataaaag aaaaagttaa ccctttattt aaccaacaaa aacgaagcaa tataaataga
      901 ctgctacgaa atcttttttg actcattcga ctgcttttaa aatagaaatt actcgcatga
      961 ttatctaata tccccatgac taaaataaaa taattgagta gtatgacata aattacaact
     1021 tgtccttgtt ctatagacaa tagtcctata gataaaaata agaacaattt acttataatt
     1081 gaaaacaaac taggtagaat taaagaaata atttgaatag ctatcaaaca aagagtaatg
     1141 gataaataaa aatatccaat aatatctaga aaatacaaaa taaataaatg aatgattgta
     1201 aaatttggaa agaataatat ttcttgacaa tggcaagaag atagagaaaa atatagtttt
     1261 tccttatata gaaagtattt atttttttac tttggatagg cagtttcata ttataacgat
     1321 tcaaagtagc gtaatctact accaaaaaaa gtaaaaataa tttcccttga aaaggaagat
     1381 agcttttaaa atagattgac aaacttatga taacaaaaaa caaaagggtt atcccaataa
     1441 aattaagata acctaaaata ctttcacatt cttttttaga aagtattttc atctagttct
     1501 accatagagc tgctttagca gcacctttct ttttcaatat ataaagaact gttgctgcaa
     1561 tacccgaacc aatagaacct actcctgtga ttgcacctac aattgaaatt actgctgtaa
     1621 ctgtactata agcactaatc aaattgacta cagtagcagc agcttttgtc gaaattccta
     1681 atgtcccagc caacatagga aaagaaacaa cacctaagct taaccaacta atgactgcta
     1741 acattgctga aacaataaga aatttattat gaaattccat tcttttagat ttcattttag
     1801 tgtactcctt ttgtatatta tgtatgtttt attgagagtt aattcgcaca tgttgtattc
     1861 ctaactctct tcaaatgttc gtctatattt tatactactt ttattaacat atctataata
     1921 aataaattac tactacattt tcctagatta tcaaattact tttatatcac taagactcaa
     1981 taaaagataa tgtctattta acttttatac gattactacc ttctttttag tttacacttc
     2041 ctacatcata aataaacata ctcaaccctc acaatctcca aataactctt ctatatcatt
     2101 gaaaattgag tttcatcctc tatactaatg taggacattc ctttaatcca atataagaat
     2161 accttgttcc attaatctct acccgatatt atagatagaa ccaaattcct atacttaaaa
     2221 ggacaaataa cgttccaacc ataataaacc tcctttcttt ttgagaacgt tttctttata
     2281 ggtttagtat agcaaaaaca tgaaaaaaac aacatgactt agaagtcagg ttgttgggaa
     2341 atctatctgt ttttctgcta aaattctctg aactaatacc tgatcgtcta gcaattctgc
     2401 tagagaaatc gccttttcat agaaactttc tgcctctaaa atattcttgt ctacaaataa
     2461 agcatatttt gcctttagca aaaatagatg aggtcggtaa aaaacatgtc ttgttttagc
     2521 aataattttc tcaaatgttt gaatatatat cagactatct tctgtctgaa tttggataaa
     2581 taggctcaga agaacttttt ccatttgaac taggctagcc ttatcttcat attctgaaca
     2641 tagcaatact ttttgagcta actcctcaaa atgttcttta tcttctaatc ccatagcaca
     2701 acaaaagaaa tacaattcaa taatcaacaa atcatttaca gaaaaatatt tcttttttaa
     2761 taactgctgg aaatattctt ccactaaccc taaaccgtag gtcacatctg aactaccata
     2821 aatatcaaaa cgagcttgtg tcacatccac aattaactgc tcttcctcag gcaaattctc
     2881 ataatagttt tcatatattt cctcaagtat agcttctttt cgttcgattc ttcccttatc
     2941 agcgtaggtt tgagaatgaa tcagctcatc tttgagtttt aggaatcctt ttggaatctc
     3001 tataacctta acatccacaa tattctgtaa tgatactcca agtttatccg caatcaataa
     3061 taatttagga agtgttgcta aagcttgccc attttcaata cgtgctagct gacgaatcgt
     3121 caactctgat tcatcaccac aaatatctat ctgagttttt ccacaagatc gcctaatagt
     3181 tttgatttta taaccaattt cacgtttcaa ctcatccata gtaatactcc ttaagttgat
     3241 tacaaagatt tctttttctt cctaattctt ttgtcaatca tatataaaac gataagcgaa
     3301 aacagcggta gaacaatata actcttttca ctcattatca gctgaaatcc cagggttgct
     3361 agtatcttaa agatgaaaat attcatcaaa aatagaaaaa caaatactaa aaaatatttt
     3421 aaaaccttct tcatttcttt tcttcaactt tcctaatttc tacacctatt ttatcgaaaa
     3481 tatatataaa actccaaata aacacttcaa ctgcactttt tttggcaaaa aatagacaga
     3541 acaaacatcc tacctactaa aaatatgata tgaaaggaaa atgatttttt actgacaccc
     3601 taggcaagct tgggacaaat aaatcattga tagcaacatt tacgacatcc tatttaaagt
     3661 tccttcaatt tctaatgtcc ccgctatttc taagccacct ctggtatgat ctattcctag
     3721 tcagaataga taacaccgtt ctaaattacg attcatagtt actccttttt tgactcgtta
     3781 ctataagact cctatttaca tgcgcaaaat aattttgatt cagattcatc atttatagtt
     3841 ttgattattt tttaacagat ttttctgttt tcttctccga agactttaaa aatttttttc
     3901 taattaaatc atccatataa ggaccaaata taccaactac taaaccaata ataaaacttt
     3961 taaaatccat aattaccacc aacatgtcgc tgcataggcc acacctccaa gtatagctcc
     4021 tcccacagca ccagttgctg caccttgcca tgttcttgtt ttaattccta gctgaagacc
     4081 tcgtgctgct cctcctccaa cacctgcttt ggcaaaatct ccccaattgc atccgccacc
     4141 ttcaacgcaa gcaagcatct cagtatccat aatttcaaat tgtgacatcg tttttgtatt
     4201 cataacgaat acctcttttt tatttttaat atttgtcttg ttacaaactt gacaagttta
     4261 gtataacagt atctattaat ttttttcatc caaatcttga attggcatcg aaacgtcttg
     4321 aattagcttt tttgtttcaa aatcatctct atttttaaaa aagatgtttt ctaatcactt
     4381 ttttactatt agacttcctg caaaactaga atcctagttc atgat
//
LOCUS       DBEROU010000082         4423 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.082, whole genome shotgun sequence.
ACCESSION   DBEROU010000082 DBEROU010000000
VERSION     DBEROU010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.082"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(56..1066)
                     /locus_tag="ACL8CL_002071"
     CDS             complement(56..1066)
                     /locus_tag="ACL8CL_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HGS1331234.1"
                     /translation="MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLL
                     HPFLSSYKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALI
                     IAYLFQRFFALDTKLATLVGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLT
                     RTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIG
                     IF"
     gene            1215..2384
                     /locus_tag="ACL8CL_002072"
     CDS             1215..2384
                     /locus_tag="ACL8CL_002072"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HGS1331235.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPATFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            2381..3151
                     /gene="recO"
                     /locus_tag="ACL8CL_002073"
     CDS             2381..3151
                     /gene="recO"
                     /locus_tag="ACL8CL_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HGS1331236.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKRMFFVKHAGQS
                     KLASVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMEAGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            3148..4140
                     /gene="plsX"
                     /locus_tag="ACL8CL_002074"
     CDS             3148..4140
                     /gene="plsX"
                     /locus_tag="ACL8CL_002074"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HGS1331237.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLVADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            4146..4379
                     /locus_tag="ACL8CL_002075"
     CDS             4146..4379
                     /locus_tag="ACL8CL_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HGS1331238.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
BASE COUNT         1285 a          835 c         1141 g         1162 t
ORIGIN      
        1 aacatatata cggcaaggcg acgttgacgt ggtttgaaga gattttcgaa gagtgttaga
       61 aaatgccgat aagggtctgc ataccaagag tggtgaggat gatggcaatc cagcagatgg
      121 ctccaagaag aatggatttt ccactggatt tgaccatagc gaccagattg gttttgagac
      181 cgatagcact catggccatg acaataagga atttagagag ttgtttgaga ggggtaaaga
      241 aactactgga cacacctaga gaggtgagta gtgtagtgag gagagaggca aggataaagt
      301 aaaggataaa aagtgggaag acttttttca gttgtaggct ttgcttgttt tcttgttggc
      361 gactttgcca gtaggataga aagagcgtga tagggataat ggccaaagta cgtgtgagtt
      421 taacaatggt tgcagactcg agggtattgc tttggtaaag actgtcccaa gcgctggcgg
      481 cagccgttac agaggaagtg tcgttgaccg cagtccctgc aaagagggcg aagccttcat
      541 tggatagatg aagccaggtg ccgagggttg gaaagatgag cgcagccaag acattgaaga
      601 aaaagataac ggaaatggct tgggctactt ccttttcctt agcatggata acaggcgctg
      661 tcgctgcaat ggcagaaccc ccacagatag aagaacctac tccaaccaag gtagccagtt
      721 ttgtatccag tgcaaagaaa cgctggaaga ggtaggcaat aatcagagct attgagatag
      781 tggacaggat gacagggagt gaagattggc caactgcgaa gacctgcgag atattgagac
      841 caaaaccaag caagacaacg gcatattgga gcaacttctt ggaactaaag gtcaaaccag
      901 catccagttg tttataggac gagagaaagg gatgtaggag catgcctatg aaaatggcaa
      961 aaacgggcgc gccaatcaca gggaagaatc ctcctaagta ccaagatagg atggaaatga
     1021 gaaggcaggc caagatacct gctccatttt ttgatagaaa tgacataaaa acctccaaat
     1081 aaaatctatt accattatag acctataaac aggaaaagta aaatagaaag tggaaagcta
     1141 ttctctaatg tatttttgcg gtcggggggc ttttgtagta taatagagat acgttttgaa
     1201 agtaggaggt atctatggac ttaactaagc gctttaataa acagttagat aaaattcaag
     1261 tttcgttgat tcgtcagttt gaccaggtta tttcggagat tcctggggtc ttgcgtttga
     1321 ccttggggga acctgatttt acaacgccag accatgtcaa ggaggcggcc aagcgagcga
     1381 ttgatcagaa ccaatcctac tatacaggga tgagtggtct gctgactcta cgtcaggcag
     1441 ccagtgactt tgttaaggaa aagtaccaac tggactatgc tcctgaaaat gaaatcttgg
     1501 ttacaattgg ggcgacagag gctttatctg cgactttgac ggctattttg gaagagggag
     1561 acaaggtact tttgccagct cctgcttatc caggctatga accgattgtt aacttagttg
     1621 gggcagaaat tgttgagatt gatacgactg aaaatggttt tgtcttgact cctgagatgt
     1681 tggagaaggc cattttggag cagggtgata agctcaaggc ggttattctc aactatccag
     1741 ccaatccgac aggaattacc tacagtcgag agcagttaga ggccttggca gctgttttac
     1801 gcaagtacga aatttttgtt gtctgtgatg aggtttactc agaattgacc tacacaggcg
     1861 aagcccatgt gtctctagga acgatgttga gagaccaggc tattattatc aatggtttgt
     1921 ctaaatcgca tgccatgaca ggttggcgtt tggggctgat tttcgctcct gcgaccttca
     1981 cagcccagtt aatcaagagt caccagtact tggtcactgc cgcaaatacc atggcgcaac
     2041 atgctgcggt agaagccttg acggctggta aaaacgatgc ggagcccatg aagaaggaat
     2101 atatccaacg tcgggactat atcatcgaaa aaatgactgc tcttggtttt gagattatca
     2161 aaccagacgg tgccttctat atttttgcta aaattccagc gggctacaat caagactcct
     2221 ttgcttttct gaaggatttt gctcagaaga aggccgttgc ctttatccct ggtgcagcct
     2281 ttggacgtta cggggaaggc tacgtccgcc tatcttatgc agccagcatg gagactatca
     2341 aagaagccat gaaacgactt gaggagtaca tgagagaagc atgattcagt ctatcacgag
     2401 tcaaggcttg gtgctttaca atcgcaattt tcgtgaggat gacaagctcg tcaaaatttt
     2461 tacagagcag gttggcaaac gcatgttttt tgtcaaacac gctggtcagt ctaagctggc
     2521 gtctgttatt cagcccttgg tgctggcacg atttctcttg cgaatcaatg atgacggact
     2581 cagttacatc gaagactatc atgaggtcat gacttttccc aagattaata gtgacctctt
     2641 tgtcatggcc tatgcgacct atgtggcagc tcttgcagat gctagtttgc aggacaatca
     2701 gcaggatgct cccttgtttg cttttttgca aaagactttg gagttgatgg aagcaggctt
     2761 ggattatcag gttttgacca atatttttga aattcaaatt ttgactcgat ttggaatcag
     2821 cctcaatttt aatgagtgtg tcttctgcca tcgggttggt caggcttttg acttttcttt
     2881 caaatatgga gcctgcctct gtccagagca ttatcatgag gataagagac gttgtcatct
     2941 caatcccaat atcccctatc tgctcaatca atttcaagct attgattttg agactttgga
     3001 gaccatttcg ctcaagcctg gaatcaagca agagctacgc caatttatgg atcaattata
     3061 tgaagagtac gttgggattc acctaaaatc aaagaaattt attgattccc tagcagactg
     3121 gggacaatta ctaaaagagg aaaagaaatg aaaaaaatcg cagtagatgc catggggggc
     3181 gattacgcac ctcaggccat tgttgagggt gtcaatcaag ccctatctga cttttcagat
     3241 atcgaggttc aactttacgg agatgaagct aaaatcaagc aatatctgac agcgacagag
     3301 cgcgtcagca ttatccatac ggatgagaag attgattcgg atgatgaacc tacgagagct
     3361 attaggaata agaaaaatgc cagtatggta ttggcagcca aggctgtcaa agatggtgaa
     3421 gcagacgctg tcctttcggc tgggaataca ggtgccttgt tggctgctgg attcttcatc
     3481 gtgggtcgta tcaagaatat cgaccgtcct gggcttatgt cgactttgcc gactgtagat
     3541 gggaagggat ttgacatgct agacctcggt gccaatgcgg aaaatacagc ccagcacctc
     3601 catcaatacg cggttctagg atccttctat gctaaaaatg ttcgtggcat tgcgcaacca
     3661 cgtgttggct tgctcaataa cggaacagag agtagcaagg gcgatccgct tcgtaaggaa
     3721 acttatgaat tactagtggc tgatgaaagt ttgaacttta tcggaaatgt ggaagcgcgt
     3781 gatttgatga atggcgttgc agatgttgtt gtggcagatg gtttcacggg aaacgctgtg
     3841 ctcaaatcca tcgaagggac agctatggga atcatgggct tgctcaagac agctattaca
     3901 ggtggtggtc ttcgagcgaa actaggtgcc ctccttctca aggacagcct cagaggtttg
     3961 aaaaaacagc tcaattattc agatgttggt ggagcggtct tgtttggtgt taaggcacct
     4021 gttgtcaaga ctcatggctc aagcgatgcc aaggctgttt atagtacgat tcgccagatt
     4081 cgtaccatgc tagaaacaga cgtagttgcc cagactgcgc gtgaattttc aggagaataa
     4141 aagagatgac agaaaaagaa atttttgacc gtattgtgac cattatccaa gagcgacagg
     4201 gagaggactt tgtcgtgaca gaatccttga gtctgaaaga cgatttggat gcggattctg
     4261 ttgacttgat ggagtttatc ttgactctgg aagatgaatt tagtatcgaa atcagcgatg
     4321 aagaaattga ccaactccaa aacgtaggag atgtggttaa aatcattcaa ggaaaatagc
     4381 aatcggagtt ccaagtcaac ggaagtagat ggtttttaga aat
//
LOCUS       DBEROU010000083         4198 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.083, whole genome shotgun sequence.
ACCESSION   DBEROU010000083 DBEROU010000000
VERSION     DBEROU010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.083"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            152..397
                     /locus_tag="ACL8CL_002076"
     CDS             152..397
                     /locus_tag="ACL8CL_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331239.1"
                     /translation="MKAFYITIEKFIRDDESLKCYFIKVISSRSKVTSLDQIEADKTI
                     QRKYSSELKKFIGFYNEIICEENSFLHVRKRWSSWFR"
     gene            798..1790
                     /locus_tag="ACL8CL_002077"
     CDS             798..1790
                     /locus_tag="ACL8CL_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1331240.1"
                     /translation="MAMIEVEHLQKNFVKTVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPNSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYEGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1792..2610
                     /locus_tag="ACL8CL_002078"
     CDS             1792..2610
                     /locus_tag="ACL8CL_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1331241.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2612..3397
                     /locus_tag="ACL8CL_002079"
     CDS             2612..3397
                     /locus_tag="ACL8CL_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HGS1331242.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3436..3714
                     /locus_tag="ACL8CL_002080"
     CDS             3436..3714
                     /locus_tag="ACL8CL_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HGS1331243.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTGFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3699..3896
                     /locus_tag="ACL8CL_002081"
     CDS             3699..3896
                     /locus_tag="ACL8CL_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HGS1331244.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFLQLWNPQNPCQILIKKLE"
BASE COUNT         1178 a          713 c          897 g         1410 t
ORIGIN      
        1 gtacgaaaca attgctaaaa catttataga aattaatttt actttcccaa tcgatttgtt
       61 ctcatcttat ttcaatccgc tatatattat ggtatcgaat cttcatcaga atgataaaat
      121 taatcaattg atatctgatt acaaacagaa tatgaaagct ttttatatca ctattgaaaa
      181 atttatacga gatgatgaaa gccttaagtg ttattttata aaggttattt caagtcgttc
      241 caaggtaaca agtctagatc agattgaagc tgataaaacg atacaaagaa aatattcaag
      301 tgagctaaaa aaatttattg gattttataa tgagattatt tgtgaggaaa atagtttcct
      361 acatgtacga aagaggtggt cgagttggtt taggtagtcg atgcgtgagt tgataattct
      421 cagggtatgg acttcttttt catgaatgag gtaaaagagc aggtattgtt tagagacaat
      481 cattctgagc atattttctg gatagaggga gtatccgatt ttatgatcaa agttaatacc
      541 gccctctggt gagaagataa gtaggttggt aatttaaact attaaacaga atttttgatt
      601 aaaagtatta ttttatgaga gaaatcctaa tttcacaatc cataggcaaa cgcttgcatt
      661 tcgtttttta ttggactata ataggttggt ataaagcctt ttgtagtaat aaaatgtaga
      721 aggtgtagaa agcaaggatt tagaatattt gtagttaaaa acacaatgtt gctattcctt
      781 acgataggga gatagatatg gcaatgatag aagtggaaca tcttcagaaa aattttgtga
      841 agactgttaa ggaaccgggc ttgaaggggg ctttgcgctc ctttattcat cctgaaaagc
      901 agacctttga agcggtcaag gatttgacct ttgaggttcc aaaagggcag attttaggat
      961 ttatcggggc aaatggtgct gggaagtcga caaccattaa aatgctgaca ggaattttga
     1021 aaccaacatc tggtttttgt cggattaacg gcaagattcc ccaggacaat cggcaagatt
     1081 atgtcaaaga tattggcgta gtctttggac aacgcaccca gctatggtgg gatttggctc
     1141 tgcaagagac ctacactgtc ttaaaagaga tttatgatgt gccaaactcg ctctttcata
     1201 agcgcatgga ctttttgaat gaagtcttgg atttgaagga ctttatcaag gatcccgtgc
     1261 ggactctttc actgggacaa cggatgcggg cggatattgc ggcctccttg ctccacaatc
     1321 ccaaggttct ttttttagat gagccgacca ttggtttgga cgtttcggtt aaggataata
     1381 ttcgtcgggc aattactcag atcaatcaag aggaagaaac taccattctt ttgaccactc
     1441 acgatttgag tgatattgag caactttgtg atcggatttt catgattgac aaggggcaag
     1501 agatttttga tggaacggtg agccaactca aggagacctt tggtaagatg aagactctct
     1561 cttttgaact gctaccaggt caaagtcatc tcgtctctca ctatgaaggt ctgtctgata
     1621 tgaccattga tagacaagga aacagcctca acattgaatt tgatagttct cgctaccagt
     1681 cagctgacat tatcaagcaa accctgtctg attttgaaat ccgcgatttg aagatggtgg
     1741 atacggatat tgaggatatt atccgtcgct tctaccgaaa ggagctctag gatgatcaaa
     1801 ttgtggagac gttataaacc ctttatcaat gcaggggttc aggagttgat tacttaccga
     1861 gtcaacttta ttctctatcg gattggcgat gtcatggggg cttttgtggc cttttatctc
     1921 tggaaggctg tctttgattc ttcgcaagag tctttgattc agggcttcag tatggcggat
     1981 atcaccctct acatcatcat gagttttgtg accaatcttc tgactagatc cgattcgtcc
     2041 tttatgattg gggaggaggt caaggatggc tccattatca tgcgtttgtt gcgaccagtg
     2101 cattttgcgg cctcctatct tttcaccgag cttggttcca agtggttgat ttttatcagc
     2161 gttggccttc catttttaag tgtcattgtc ttgatgaaaa tcatatcggg tcaaggtatt
     2221 gtagaggtgc taggattaac tgtcctttat ctttttagct taacgctcgc ctatctgatt
     2281 aactttttct ttaatatttg ctttggattt tcagcctttg tgtttaaaaa tctttggggt
     2341 tccaacctac ttaagacttc catagtggct tttatgtcgg ggagtttgat tcccttggca
     2401 ttctttccaa aggttgtttc agatattctt tcctttttgc ctttttcatc cttgatttat
     2461 actccagtta tgatcattgt tggaaaatac gatgccagtc agattcttca ggcactcctt
     2521 ttgcagttct tctggctctt agtgatggtg ggattgtctc agttgatttg gaaacgggtc
     2581 cagtccttta tcaccattca aggaggttag tatgaaaaaa tatcaacgaa tgcatctgat
     2641 ttttatcaga caatacatca aacaaatcat ggaatataag gtggattttg tggttggtgt
     2701 cttgggagtc tttctggctc aaggcttgaa tctcttgttt ctcaatgtca tctttcaaca
     2761 tattccattc ctagaaggct ggacctttca agagatagct ttcatttatg gattttcctt
     2821 gattcccaag ggaatggacc atctcttttt tgacaatctc tgggcactag ggcaacgcct
     2881 agtccgaaaa ggagagtttg acaagtatct gactcgtccc atcaatcctc tctttcacat
     2941 cctagttgaa acctttcaga ttgatgcctt gggtgaactc ttagtcggtg gtattttatt
     3001 gggaacaaca gtgaccagca ttgtttggac tcttccaaaa ttcctgcttt tcctagtttg
     3061 tattcctttt gcgaccttga tttatacttc tcttaaaatc gcaacagcca gtatcgcctt
     3121 ttggactaag cagtcaggcg ccatgattta catcttctat atgttcaatg actttgctaa
     3181 gtatccgatt tctatttaca attctcttct tcgttggttg attagcttta tcgtgccttt
     3241 cgcctttaca gcctattatc cagctagcta tttcttacag gaaaaggatg tgttctttaa
     3301 cgtaggaggt ttgatgttga tttctctggt tttctttgtt atttccctta aactttggga
     3361 taagggctta gattcctacg aaagtgcggg ttcgtaaaag ctaaagtaag actaaaatca
     3421 agaaagaaac ttatgatgtt tgtaattgaa gaagtcaagg atgaaaatca aaaaaaggca
     3481 gttgtagctg aggttttgaa ggatttgcca gaatggtttg gaatcccaga aagcacacaa
     3541 gcctatatag aaggaaccac gacactgcaa gtttggaccg cctatcagga gagtgatttg
     3601 actggatttg taagcttatc ctattcgagt gaagattgtg cagagattga ttgtctcggc
     3661 gtaaaaaagc ttatcaaggt agaaaaattg ggagccaatt gcttgctact ttagagagtg
     3721 aagctcgtaa aaaagttggt tatctgcagg tcaaaacagt ggcagaaggt tctaataaag
     3781 attatgatcg aacaaatgac ttttatcgag gtcttggctt taaaaagtta gagatttttc
     3841 ttcaactatg gaatccgcaa aatccttgtc agattttgat taaaaagctt gaataatatt
     3901 acttgacatc tattctcaga gtgctatact gtaagtgtaa tcgccgattt agcttagttg
     3961 gtagagcaag gcactcgtaa agcctaggtt ataggtagat aaacgactga ggatttgaaa
     4021 aaatagatag gtagaagata accgttaagc cttattctta gcggttattt atattgttta
     4081 atagcgctga tatttcatca attatgcctg ttttcgtgtt tctggtagtt gttcaagttt
     4141 attgctacta tttttgatgg tatgaatgtg cttataatgt atccctgtta agtacgaa
//
LOCUS       DBEROU010000084         4180 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.084, whole genome shotgun sequence.
ACCESSION   DBEROU010000084 DBEROU010000000
VERSION     DBEROU010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.084"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..160)
                     /locus_tag="ACL8CL_002082"
     CDS             complement(<1..160)
                     /locus_tag="ACL8CL_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HGS1331245.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFSGNPTTF
                     NYLLDYYAD"
     gene            complement(319..1926)
                     /locus_tag="ACL8CL_002083"
     CDS             complement(319..1926)
                     /locus_tag="ACL8CL_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HGS1331246.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNGDGVGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKQVLAPWDAFCVELL"
     gene            2079..3563
                     /locus_tag="ACL8CL_002084"
     CDS             2079..3563
                     /locus_tag="ACL8CL_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HGS1331247.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQVEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFLNQLEKNGIDSYLHYPIKGYPTDMDHIISPEGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVGEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            3658..4140
                     /locus_tag="ACL8CL_002085"
     CDS             3658..4140
                     /locus_tag="ACL8CL_002085"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HGS1331248.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWAKLILEQGISDDAFERHVI
                     "
BASE COUNT         1216 a          961 c          799 g         1204 t
ORIGIN      
        1 tatcagcgta atagtctaat agatagttaa atgtagttgg atttccacta aaggttgatg
       61 agtaagtttt tgtatctgaa cctgattggc cgcaagcagc taaaagcaaa gcagatgcaa
      121 aagtcaggcc tgcactaagg acacgcttct ttatgttcat cttctttctc cttaatagtg
      181 ggaatttgta aagttaattg aatttcaaga atgaaagttt ataaactttg gttataaaaa
      241 acaaaggatt tctgtctttt atacagtcct cccttgtttt tatacgattt caattttaaa
      301 tttttctgca aaaaatattt atagtaattc cacacagaaa gcatcccatg gagccaagac
      361 ctgtttttca agtacttctt tagccgcagt gttttcaatc aagacagatt tgacttttcc
      421 ttctactgtc aagtcttgct cttcattgga caagttagcc acaactagga agcgacggtc
      481 gccatcctta cgtatataag caaagacctt atcagccgta tcaagcaatt caaagtcagc
      541 tcgaactagc cagctattct ccttgcgaat ttggaccagt ttctgatagg tatagaaaat
      601 agaatctgga tttgccagcg cttcttggac attgatcatc tcgtaatttg gattaaccgc
      661 caaccaaggt tgacctgttg agaaaccagc gtttttgctc tcgtcccatt gcataggggt
      721 acgggcattg tcacgtccaa taacacggat actgtccatg atttcttcaa tcggaacacc
      781 tttttcaaga gcctcacgcg catagttgag agattcaata tcttctactt gatccagtgt
      841 ttcaaacgga tagttggtca tcccaatctc ctcaccttgg tagatataag gagttcctct
      901 cataagatga agcaagattg caaaggcttt ggcagatttt tcgcggtatt cttggtcatt
      961 tccccagatt gagacgatac gagggaggtc atgattgttc cagaagaggg aattccaacc
     1021 gtcctcaact cctaactctg tctgccattt gttgaagatt tcttttaact tagcgatatt
     1081 cagctctttt tgatagtgcc atttaggctg accttcctga tactgaagac cgatatgttc
     1141 aaactggaag accatagaca attcttgccc ctttggatca gagtaaagct tggcaatctc
     1201 tggcgttgct ccccaagtct cccctactgt caagagatcc ttatctccaa aggtcgcctg
     1261 attcatttcc ttgagatagg gatggagcat aggaccatta ttgactacct tctcgtcagg
     1321 aattttgcca atcatgtcaa taacatccat acggaaacca ccaatacctt tatcaatcca
     1381 gaagttcatc atctcataaa ttttctggcg aagtttttca ttttcccagt tgaggtccgg
     1441 ctgtttcttg ctgaaaaagt ggagatagta ttgacctgac ttttcatcgt attcccaagc
     1501 agacccacta aagatagaat ctaggtcatt gggttcatcg cgccagatat agtagtctcg
     1561 ctcagggctg tcagtatttt cacaggcttc gacaaaccaa gcatgttcat ctgaggtatg
     1621 attaaccacc aagtccatga tgatacgaat gtcacgcttc ttagcttctg caatcagctg
     1681 atccatgtcc tccatggttc caaaaatagc cgcaatcgct tgataatcag caatatcata
     1741 gccattatca tccatagggc tgtcataaac gggagaaagc caaattgctg tgattcctag
     1801 cttggctaga tagtccaact tactggtaat acctggcaaa tcaccaactc catctccatt
     1861 actatccata aaactctttg gatagacttg atagactacg gcattgtgcc accatttttc
     1921 ttgcatctgt ttacctcatt attttaataa tctatagtat atgatagcac tttttaaaat
     1981 tttgtgcaag cgtttgctta atctttttaa tttatttttt agctccgtag tttcctaact
     2041 tccaattttt tattataata gagtccagag gtaaaaaaat gaaaaaacaa gcttttagtt
     2101 ctgaacagta tttgaattta caacgcgacc atattttgga gcgcattaac caatttgacg
     2161 gcaagctcta cttagagttt ggcggtaaaa tgttagaaga tttccacgct gctcgtgtcc
     2221 ttcctggtta tgaacctgac aacaaaatca agctcttgca agaattgaaa gagcaggttg
     2281 aggttgtgat tgccattaat gctagcaaca ttgaacattc caaagcacgt ggcgacttgg
     2341 gcatttctta tgaccaagaa gttcttcgtt tgattgacaa attcaatgaa ttagggattt
     2401 ttgttggttc cgttgtcatt acacaatacg ctggccaacc cgctgcagat gccttcctca
     2461 accaacttga gaaaaacgga attgattctt atcttcatta tccaatcaaa ggatatccga
     2521 cggatatgga tcacatcatt tccccagaag gcatgggcaa aaacgactac atcaaaacca
     2581 gtcgcaactt gattgtcgta accgctcctg gacccggttc tggaaaattg gcaacgtgta
     2641 tgtccaatat gtaccacgac caaatcaatg gcataaagtc tggctacgct aaatttgaaa
     2701 ccttccctgt ttggaatctt ccccttcatc atccagttaa cttggcttat gaagctgcca
     2761 cagctgacct tgatgatgtc aacatgattg accccttcca tcttcaaacc tatggagaaa
     2821 ccactgtcaa ctacaaccgt gatatcgaaa tcttcccagt gctcaagcgc atgttggaac
     2881 gtattctcgg aaaatcacca tacgcttcac cgacagatat gggtgtcaac atggttggtt
     2941 tcgctattac agatgacgag gctgctgtcg aagcttctaa acaagaaatc atccgccgtt
     3001 actatcaaac agttcttgac ttcaaagctg aaaaagttgg cgaagctgcc gtcaagaaaa
     3061 ttgagttgct catgaacgac ctcggtatca cacctgcaga ccgtaaggtt gctgtcgttg
     3121 cgcgccaaaa agcagaagaa actggtggac cagccctagc ctttgaattg ccaaatgggg
     3181 aaatcgtcac tggtaagaac tcagaactct ttggccctac agccgctgcc ttgatcaacg
     3241 ccatcaaaaa atcagctgac atcgctaaag aagtaaaact aatcgagcct gaagttgtta
     3301 agccaatcca aggtcttaaa atcgatcatc tcggtagccg caatccacgc cttcattcaa
     3361 atgaaatcct gattgcactt gctatcacag ctacagaaaa tcctgatgct gcccgcgcta
     3421 tggaagaact cggcaacctc aaaggaagcg aagcccactc aaccatcatc ttgactgatg
     3481 aagacaagaa tgtccttcgt aaactgggta tcaacgtaac ctttgaccca tactaccaat
     3541 acgaccgctt atatcgtaag taaagatttc aacctctcta gcctcggaga ggttttctct
     3601 ctgtctcagg aacaaccttt atgatataat gagagaagaa aactgaaagg atttaccatg
     3661 tcaaaagaag ttattgtcga aagttttgaa cttgaccaca ccattgtcaa agcaccctat
     3721 gttcgcttga ttggggaaga aacaggacca aaaggagaca tcatctccaa ttatgatatt
     3781 cgcttggtgc aaccaaacga agactctatc cctactgccg gccttcacac tatcgagcac
     3841 ctcttagcca aactcatccg tacccgcatt gacggcatga ttgactgttc accatttggt
     3901 tgccgcacag gcttccacat gattatgtgg ggacgtcaca ccagtgctaa aatcgcagct
     3961 gttatcaagg attcgctcaa ggaaatcgct gaaactacta cttgggaaga tgtcccaggg
     4021 acaaccatcg aatcttgcgg aaactacaag gaccacagcc tcttctctgc taaagaatgg
     4081 gcaaaactca tcttggaaca agggatttca gatgatgcct ttgaacgtca tgtgatttaa
     4141 aatcgagtag gggataatct ctagcccttc tcacaccacc
//
LOCUS       DBEROU010000085         3635 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.085, whole genome shotgun sequence.
ACCESSION   DBEROU010000085 DBEROU010000000
VERSION     DBEROU010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.085"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..300
                     /locus_tag="ACL8CL_002086"
     CDS             <1..300
                     /locus_tag="ACL8CL_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331249.1"
                     /translation="QNRLHNIYKGFTHYKYYRAENSHLIYADYFEMKLKKLLKDDTKV
                     FEKSTFKFVEGYKIYLTESKESGIKQMDNVIKYFEFIESKSIALYFQKRLNELID"
     gene            complement(654..1187)
                     /locus_tag="ACL8CL_002087"
     CDS             complement(654..1187)
                     /locus_tag="ACL8CL_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HGS1331250.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(1276..2052)
                     /gene="recX"
                     /locus_tag="ACL8CL_002088"
     CDS             complement(1276..2052)
                     /gene="recX"
                     /locus_tag="ACL8CL_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HGS1331251.1"
                     /translation="MKITKLEKKKRLYLMELDNGDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDINENIVSQVIANLKEEKW
                     INDSQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            2090..3457
                     /gene="rlmD"
                     /locus_tag="ACL8CL_002089"
     CDS             2090..3457
                     /gene="rlmD"
                     /locus_tag="ACL8CL_002089"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HGS1331252.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYNEAVKALDVNKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDIPYIQSVDMFPHTARTEAVVKLIKK
                     IRKSS"
     gene            3507..3578
                     /locus_tag="ACL8CL_002090"
     tRNA            3507..3578
                     /locus_tag="ACL8CL_002090"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3540..3542,aa:Glu,seq:ttc)
BASE COUNT         1204 a          619 c          643 g         1169 t
ORIGIN      
        1 caaaataggc tccataatat ctataaggga tttacccact acaaatatta tagagccgaa
       61 aattcacatc taatatatgc agactacttt gaaatgaaat taaaaaaatt attaaaggat
      121 gacacaaaag tttttgaaaa atctacattc aaatttgtag aaggatataa aatatacctg
      181 acagaatcta aagaatctgg aattaaacaa atggacaatg tcataaaata ttttgagttt
      241 attgaatcta aaagtattgc tttatatttt caaaaacgat taaatgagct gatagattaa
      301 atagcatttt ctctgttgag atattgtttt taaaatattg tactaaatga ttgatgctat
      361 gtggaaatac aaaaaaatgt ttttgatacg aagttgacct gtatttttta tactaatcat
      421 tttcgtattt tttgtattaa acgatataag tttgttgtaa acttacaagg aataaagaca
      481 ttaaaaaata acagtatatc tatttgtttt atatatttta cgaattctgc ataaatctct
      541 ttctagtaat gtgttgtaac tctgctataa tagatttatt cctttttgtg tttacacaat
      601 ttattttata ctaccaaaaa aggtcaggat tttgttcctg acctttgaca actttaccga
      661 ttctttagtt ctacatagcg cttgtaccaa atgtttacat aggcttctga gaaaggacca
      721 cgtccattgt taatccaatc aacaagaatt ttgacatgtt cttttaaaat atagtccaag
      781 tcatcagaat aattcatttt gcgtttgtga cgctcgtact cttcaacgtc caagagacgt
      841 ttttccccat ctgtaaaaat tttaacatcc aaatcgtaat caatatactt cagtgcttct
      901 tcatccagat agtaggggct agccatattg caatagtaag aagttccatt atcacgaatc
      961 atggcaatga tattaaacca atatttcttg tgaaagtaaa caatagccgg ttctcgagtg
     1021 acccaacgac gaccatcact ttcggtaaca agtgtatgat cgttgacacc aataatggcg
     1081 ttttctgttg tttttagtac catggtgtcc cgccaagttc ggtggagact cccatcatgc
     1141 ttataacttt gaattgtaat aaagtcgcct tcttttggaa gcttcataac taaccaactt
     1201 tctacaattt ataagtttat catttactat tgtaccataa aattacccaa aatctgtgaa
     1261 tttcacttgg aaatattaaa gatattctct aagagcgctt gctatatccg aaaaatcgta
     1321 gccctttcgt gctaaaactt gagttaaacg ctgcttcagt tcgtatcctt catactttcg
     1381 ggcatactta gtatattgct tatcaagttc cttgaagatg agttcctgag tcgtttcttc
     1441 atcaacttga ctatccaatt cgtcaaaggc aattttagca tcaaaataag agaagccctt
     1501 gttagtcaag ttctggataa tcttatcttg cagggcacga gctggaagtt ttccctcata
     1561 ttttttcaat agtttattgg ctacacgttg agcaacttcc gaaaaatcaa aatcattcaa
     1621 gatttcttct atagtagatt ttgaaattcc tttttgtgct aatttctgag tcagtacata
     1681 aggtcccttg tctcctgaaa gttgattggc attgatgata gcataagcgt actggctatc
     1741 attaatccac ttctcttctt taagattagc aatgacttga gaaacgatgt tttcattaat
     1801 atcatatttt ttcagatatt ctctgacctc tttttcagta cgtgctttaa aggataagtg
     1861 gtagagggcc agattcttac cataagaaaa ttgagcaaag tcttgaatct ctttcaattc
     1921 ctcttcgctt atcaccttat ctctcgataa cataaaacga acaattgtgt cttcggtgat
     1981 atagcatttg tcgccattat caagctccat cagatagagt ctttttttct tttcaagttt
     2041 tgtgattttc atagctctat tataactcaa aatgtgataa gataggggta tgaatctgaa
     2101 agtgaaacaa aaaataccat taaaaatcaa gcgcatggga attaacggtg agggaatcgg
     2161 cttttaccaa aaaacattag tctttgtacc aggagctctc aaaggcgaag atatctattg
     2221 tcagattact tctattagac gcaactttgt tgaagcaaaa ttactgaagg tcaacaagaa
     2281 gtctaaattt cgaattgtgc catcttgtac tatttataat gaatgcggag gctgccaaat
     2341 catgcacctg cattatgata agcagctgga gttcaagacg gacttacttc atcaagcgct
     2401 gaaaaaattt gctcctgcag gatatgaaaa ttatgaaatt cgtccaacta ttggaatgca
     2461 ggaaccaaaa tattacagag ctaagttaca atttcagact cgaaaattta aaaatcaggt
     2521 caaggcgggc ttatatgcac aaaactctca ctatttagta gagttgaaag actgcctggt
     2581 acaagataag gaaacccaag tgattgctaa tcgcttagca gaattactta cttatcacca
     2641 gattccaatc acggatgaga gaaaagttct aggtgtccgt actattatgg tccgacgcgc
     2701 gagaaagacc ggacaggttc agattattat tgttacaaac cgccagctta atttaactca
     2761 attggtaaaa gagttggtta aagatttccc agaagttgtg acagtagctg ttaatacaaa
     2821 tacagctaaa accagtgaga tatatggtga aaagacagag attatctggg ggcaagagag
     2881 tattcaagaa ggtgtactca attatgaatt ttcactatcc cctcgagctt tttatcaact
     2941 aaatcctgaa caaacagaag ttctttataa cgaggcggta aaagccttgg atgttaataa
     3001 agaagaccat ttgattgacg cttattgtgg agttggaacg attggttttg cctttgcaaa
     3061 gaaagttaaa acacttagag gtatggatat tattccagaa gctattgaag atgctaagcg
     3121 aaatgctaaa agaatgggat ttgacaacac acattacgaa gcaggaacag cggaagagat
     3181 tattcctcgc tggtataagg aaggctaccg agcagatgct ctgattgtgg acccaccacg
     3241 tacaggtctg gatgataagt tattagatac tattcttacc tatgtaccag aaaaaatggt
     3301 ctatatttct tgcaatgttt caactttggc aagggatttg gtaaggttag tagaagtcta
     3361 tgatatccct tatattcagt cggtcgatat gtttccacac actgctcgaa ctgaagctgt
     3421 tgtaaaatta attaaaaaaa ttagaaaaag tagttgacaa aacataaaaa ggctgtataa
     3481 tagtaagagt tgaaaataac aactcaggtc cgttggtcaa ggggttaaga caccgccttt
     3541 tcacggcggt aacacgggtt cgaatcccgt acggactatg gtatgttgcg gttggaccac
     3601 ttgatgaaaa aaagtttaaa aaagcttaaa aatct
//
LOCUS       DBEROU010000086         3563 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.086, whole genome shotgun sequence.
ACCESSION   DBEROU010000086 DBEROU010000000
VERSION     DBEROU010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.086"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(93..945)
                     /locus_tag="ACL8CL_002091"
                     /pseudo
     CDS             complement(93..945)
                     /locus_tag="ACL8CL_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182753.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
     gene            936..1001
                     /locus_tag="ACL8CL_002092"
     CDS             936..1001
                     /locus_tag="ACL8CL_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_128650800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHP2/SHP3 family peptide pheromone"
                     /protein_id="HGS1331253.1"
                     /translation="MISKFFPILMLVMDIIIIVGG"
     gene            1105..1974
                     /locus_tag="ACL8CL_002093"
     CDS             1105..1974
                     /locus_tag="ACL8CL_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HGS1331254.1"
                     /translation="MKHKKVLIIGYGIMTRGILKILKQSKKYNIYLYSRHLNSTINGI
                     IFIREDELPDAINQVDFIITCFSNHIETDNFINEFLLNIQSLRKTIVIDFTTSRIDCV
                     EKFKKTVEIKGGYYIEAPFTGGKLGSNSGQLSIFLHIDEGVNYNKKELMELLKLISKK
                     IYFFEKSTEPTKFKLIYNSWGAYLLYSIKLFNPLQYDFSPKSLEVANTIVCNDGWMSL
                     VCNSKLEQINDQDFDDIHFRVEYMVKDLEYANFSVFHDEVKEINYLLQQYGDCLVTDS
                     GKDYTVIGKYDNL"
     gene            1982..2683
                     /locus_tag="ACL8CL_002094"
     CDS             1982..2683
                     /locus_tag="ACL8CL_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HGS1331255.1"
                     /translation="MLSKELDILISFFKKCEVGKISLVITGSLARGNPRIKDGKLESD
                     IDILVIVDSIQQLISIKKTLEGRFHFVHKISLIFCLKERINRSRYRGIINSIRSVDNL
                     LVDNLHIKNQIIEALDSPTNIVEQTRSMIQEFCYYSSKYLISKNNYLELKLEKYWKEI
                     ATLNHIDKKIKHLDFERIFAVLKEHKIQILDSSEYFFQNVKTSENIYLEMRDLVSLEN
                     QGLDFEHCILSLGER"
     gene            2680..3210
                     /locus_tag="ACL8CL_002095"
     CDS             2680..3210
                     /locus_tag="ACL8CL_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331256.1"
                     /translation="MIINTLGPTATDCFYAINRIKLKVDVIVLYDSFDSIVNCFDKLK
                     GQYILLPVAFESSIKNYSWKDFNFEYHKRIEIVDSFHSFTKGMVLIENCNYKINRAII
                     QPATEILMLNYLKKKSMDLRIDFTHSKVMANKKFLEEGYRFSIVSQETIINNDDIIIC
                     ETFSPEMIWCLYRVKG"
     gene            3214..>3563
                     /locus_tag="ACL8CL_002096"
     CDS             3214..>3563
                     /locus_tag="ACL8CL_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331257.1"
                     /translation="MFSEIIKKYDIVTLKDSGFLRGDWFIGSALMAHDYESLKEGIYF
                     YPQETIWTRILDLEYINPYWEIYNQTYNFLLVNKQDSLAMNFLTSLLREIEVISSKTL
                     DFVVITSNEHLIRN"
BASE COUNT         1300 a          401 c          607 g         1255 t
ORIGIN      
        1 atctgtctat agttaaaact acaagttcat cggagttgat aaggttttat tgagactctt
       61 gattataaaa cgttagatgg taaggaatca tttcatttat tgtttacaag tttatcaaaa
      121 tggctagtat agtgtaattt taacttgtct tctcctaaaa tatcgagaac ttgtatagct
      181 tgtttcattg tttcaatacc actacttaat tgacgtttaa attcatagta accggttgca
      241 tagagaaaaa ctgtctgttc ataaaagttt aattcattct ctgaacaatc cctttatctc
      301 attaattaaa taagagcaat tagaaaattc ttctttatct atgctgagaa tgaaacaatt
      361 tatagctagc tgagtgacaa tccgcttatt tgttttattt agagatgagt aaatataatt
      421 atttaacatt tctttagtta ataagaagta ggagttgtag ttaattgttc gtacacagtt
      481 acctaataaa ataatctcat aataacccca tttttcaact ttaaagagat aatctgctag
      541 ttgaagtaat tcatcatctg atggaatgat tcttgaatcc attgtgtgta aaatggattt
      601 taccattgtt ttttcaaaag aatctaaagt gtaatttgag gaatccgaaa gtagagattg
      661 tatattattg atgttttgta atgaatattg tttacggata tactgtatta agttggcaaa
      721 tgactcagtt tttgtatagt cttcatcgtg taaaataaga aactcatcca aagtaatgtg
      781 taatttgtct aaaatattga tcagtcggat acatgatatc tctgactctc cacgttcaaa
      841 tcgggatatt tgcgattttg aaagatgctc atcagcaaca gagcatagac tgatttgttt
      901 gcctttacga acttttctaa gtgtgatgcc aagttttgat ttcaaaattt ttcccaattt
      961 tgatgctagt gatggacatt atcattattg ttggtggtta aatcacttag gtgtacacct
     1021 aagtctttag attatctaaa gactatactt actagctaaa acatatttaa gttaaatcgt
     1081 aaaattttag agagtggtga gactttgaaa cataaaaaag tattaattat agggtatggt
     1141 ataatgactc ggggtatatt aaaaatcctg aaacagtcga aaaaatataa tatataccta
     1201 tatagtaggc atctaaattc aacaataaat ggaataatat ttattagaga agatgaatta
     1261 cctgatgcta taaatcaggt tgattttata atcacttgtt ttagtaatca tattgaaacg
     1321 gataatttca ttaatgagtt tttattaaat atacaatcac ttcgtaaaac catagtaatt
     1381 gattttacaa cctccaggat tgattgcgtt gaaaaattta aaaaaactgt tgaaattaaa
     1441 gggggatact atatagaggc tcctttcaca ggaggtaagt taggaagtaa tagtggtcaa
     1501 ctatcaattt ttttacatat tgatgaaggt gtaaactata ataaaaagga gttaatggaa
     1561 ttattaaaat taatttcaaa aaaaatatac ttttttgaaa agagtacgga accaacaaaa
     1621 ttcaagttaa tttataactc gtggggagcg tatttgcttt actctattaa gctattcaac
     1681 cctttacagt atgatttttc accaaagtct ctagaggtag cgaatacaat cgtatgtaat
     1741 gatggttgga tgagtttagt atgcaactcc aaattagaac agatcaatga tcaagatttt
     1801 gatgacatac actttagagt agaatatatg gtaaaagatt tagaatatgc taatttttca
     1861 gtctttcatg atgaagtgaa agaaataaat tatttattac agcaatatgg ggattgctta
     1921 gtaactgata gtggaaaaga ttatacggta ataggaaagt atgataactt ataggaatga
     1981 cttgctgtct aaagaattag atatactaat ttcatttttt aaaaaatgcg aagtaggaaa
     2041 aatttcactc gttattactg gttccttggc aagaggtaat ccaagaatca aggatgggaa
     2101 attagaatcg gatattgata ttctagttat agtagacagt attcagcaat taattagtat
     2161 aaaaaagact ttagagggaa ggtttcactt tgtacataaa atttctttga ttttttgttt
     2221 gaaggaaaga ataaatcgaa gtcgttatcg tggaattatt aatagtatta gaagtgtaga
     2281 taatctttta gtagataatc tgcatataaa aaatcaaata atagaagctt tagactcacc
     2341 taccaatatt gtggagcaga ctcgctctat gattcaggaa ttttgttatt attcatcaaa
     2401 atatttaatt tcaaagaata attatttgga attaaaacta gaaaagtatt ggaaggagat
     2461 tgcaacatta aatcatatcg ataaaaagat aaaacaccta gattttgaaa gaatatttgc
     2521 tgttttaaaa gaacacaaaa tacaaatttt ggattcaagt gaatattttt tccaaaatgt
     2581 aaaaacttct gagaatattt atttagagat gagggattta gtatctttgg aaaatcaagg
     2641 attagacttt gaacattgta ttttgagctt gggggaaaga tgataataaa tacattagga
     2701 ccaactgcaa cagattgttt ttatgcaata aatcgtataa aattaaaagt agatgtgatt
     2761 gtactgtatg atagttttga tagtatagtt aattgctttg ataaattaaa agggcaatat
     2821 atattattac ctgtagcttt cgagagtagt attaaaaatt atagttggaa agatttcaac
     2881 tttgaatatc ataagcggat tgaaattgtg gattcttttc actcttttac taagggaatg
     2941 gttctaatcg agaattgtaa ttataaaatt aatagggcga ttatccaacc tgcaactgag
     3001 attttgatgt taaactattt aaagaaaaaa agtatggatc tgaggataga ttttacccat
     3061 agtaaagtta tggcaaataa aaaatttctg gaagaaggtt atcgtttttc aatagtaagc
     3121 caagaaacga taattaataa tgatgatatt attatttgtg aaactttctc tccagaaatg
     3181 atttggtgct tatatagagt gaaaggatag aaaatgtttt ctgaaattat taagaaatat
     3241 gatattgtta cactgaaaga tagtggtttt ttgagaggtg attggttcat aggttcagcg
     3301 ttaatggctc atgactatga aagccttaag gaagggatat atttctatcc ccaggaaaca
     3361 atttggacaa gaatcttgga tttagaatat ataaatccgt attgggaaat atataatcaa
     3421 acttataatt tcttattagt taataaacaa gattcattag caatgaattt tttaactagt
     3481 cttctaagag aaatagaagt tatttcgtct aaaacattag attttgttgt tattacttca
     3541 aacgaacacc taattaggaa ttt
//
LOCUS       DBEROU010000087         3463 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.087, whole genome shotgun sequence.
ACCESSION   DBEROU010000087 DBEROU010000000
VERSION     DBEROU010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.087"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..174
                     /locus_tag="ACL8CL_002097"
     CDS             <1..174
                     /locus_tag="ACL8CL_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_135354309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
                     /protein_id="HGS1331258.1"
                     /translation="WVKDGDTWYYLEASGAMKASQWFKASDKWYYVNGSGALAVNTTV
                     DGYGVNANGEWVN"
     gene            complement(377..1642)
                     /locus_tag="ACL8CL_002098"
     CDS             complement(377..1642)
                     /locus_tag="ACL8CL_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HGS1331259.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIINKDTHKEIIAKLDYDAPSCP
                     ECGSQMKKYDFQKPSKIPYLETTGMPTRILLRKRRFKCYQCSKIAVAETSLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLSISTSTVIRKLNDFHFKHDFSRLPEIMSWDEYA
                     FTKGKMSFIAQDFNNLNIITVLEGRTQAIIRNHFLRYDRVVRCRVKIITMDMFSPYYD
                     LARQLFPCAKIVLDRFHIIQHLSRAMSRVRVQIMNQLDRKSHEYKAIKRYWKLIQQDS
                     RKLSDKRFYRPTFRIHLTNKEILNKLLSYSEDLKHHYQLYQLYQLLLFHFQNKEPEKF
                     FGLIEDNLKQVHPIFQTVFKTFLKDKEKIVNALQLHYSNAKLEATNNLIKLIKRNAFG
                     FRNFENFKKRIFIALNIKKERTKFVLSRA"
     gene            1845..>3463
                     /gene="pspC"
                     /locus_tag="ACL8CL_002099"
     CDS             1845..>3463
                     /gene="pspC"
                     /locus_tag="ACL8CL_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein PspC,
                     LPXTG-anchored form"
                     /protein_id="HGS1331260.1"
                     /translation="MFKSNHERRMRYSIRKFSVGVASVAVASLVMGSVVHATENEGTT
                     QAPTSSNRGNESQAEQRRELDLERDKVKKEVREYKEKKVKELYSKSTKSRHKKTVDIV
                     NKLQNINNEYLNKIIQSTSTYEELQKLMMESQSEVDKAVSEFEKDLSSSSSSGSSTEP
                     EASDTAKPNKPTELEKKVAEAQQKVEEAEKKAKDQKEEDHRNYPTITYKTLELEIAEF
                     DVKVKEAELELLKVKAKESRDEKKIKQAEAEVESKQAEATRLKKIKTDRKKAEEEAKL
                     KEAVEKNAATSEQGKPKRRVKRGALGEQATPDKKDYFEKDFRPAFNKNQQMVAIQESL
                     NKLDDETKTVPDGAKLTGEAGNAYNEVRDYAIKVVSENKKLLSQTAVTMDELAMQLTK
                     LNDAMSKLKEAKAKLVPEVKPQPENPEHQKPTTPAPDTKPIPQPEGKKPSVPDINQEK
                     EKAKLAVATYMSKILDDIQKHHLQKEKHRQIVALIKELDEFKKQALSEIDNVNTKVEI
                     ENTVHKIFADMDAVVTKFKKGLTQDTPKEPD"
BASE COUNT         1239 a          572 c          726 g          926 t
ORIGIN      
        1 tgggtgaaag atggagatac ctggtactat cttgaagcat caggtgctat gaaagcaagc
       61 caatggttca aagcatcaga taaatggtac tatgtcaatg gctcaggtgc ccttgcagtc
      121 aacacaactg tagatggcta tggagtcaat gccaatggtg aatgggtaaa ctaaacctaa
      181 tataactagt taatactgac ttcctgtaag aactctttaa agtattccct acaaatacca
      241 tatcctttca gtagataata tacccttgta ggaagtttag attaaaaaat aactctgtaa
      301 tctctagccg gatttatagc gctagagact acggagtttt tttggctctt tgtcaactgt
      361 agtgggttga agtcagctaa gctcgagaaa ggacaaattt tgtcctttct tttttgatat
      421 tcaaagcgat aaaaatccgt tttttgaagt tttcaaagtt tcgaaaacca aaggcattgc
      481 gcttgataag tttgatgaga ttattggtcg cttccagttt ggcattagaa tagtgtagtt
      541 gaagggcgtt gacaatcttt tctttatctt tgaggaaggt tttaaagaca gtctgaaaaa
      601 taggatgaac ctgcttaaga ttgtcctcaa taagcccgaa aaatttctct ggttccttat
      661 tctggaagtg aaaaagcaag agctgataga gctgatagag ctgatagtgg tgtttcaagt
      721 cttccgaata actcaaaagc ttgtttaaaa tctctttatt ggttaaatgt atacgaaaag
      781 taggacgata aaatcgctta tcactcagtt tacggctatc ctgttgaatg agtttccagt
      841 agcgcttgat agccttgtat tcatgagact ttcgatccaa ttgattcatg atttgaacac
      901 gcacacgact catggcacgg ctgagatgtt ggataatatg gaaacgatct agaacgattt
      961 tagcacacgg gaaaagctgt ctagctaagt catagtaagg gctaaacata tccatagtaa
     1021 taattttgac gcgacatcgg acaactctat cgtagcgaag aaagtgattt cgaatgatag
     1081 cttgtgttct tccctcaaga acagtgatga tattgagatt gttaaaatct tgcgcaatga
     1141 agctcatctt tccctttgta aaagcatact catcccaaga cataatctca ggaagacgcg
     1201 aaaaatcatg tttaaagtga aaatcattga gcttacgaat aacagttgaa gttgagatgg
     1261 aaagctgatg ggcaatatca gtcatagaaa tcttttcaat caacttttga gcaatctttt
     1321 ggttgatgat acgagggatt tggtgatttt tcttgacgag ggaagtctca gcgaccgcta
     1381 tttttgagca ctgatagcac ttgaatcgac gctttctaag gagaattcta gtaggcatac
     1441 cagtcgtttc aagataagga attttagaag gtttttgaaa gtcatatttc ttcatttgac
     1501 ttccgcactc agggcaagat ggggcgtcgt agtccagttt ggcgatgatt tccttgtgtg
     1561 tatctttatt gatgatgtct aaaatctgga tattagggtc tttaatgtct agtaattttg
     1621 tgataaaatg taattgttcc atatgattct ttctaatgag ttgttttgtc gcttttcatt
     1681 ataggtcata tgggactttt tttctacaca aaataggctc cataatatcc ataggggatt
     1741 tacccactac aaatattata gagccaaata atgtagcaaa tttcatatac aattcatatt
     1801 agcaggatat actgtagtta attaaaatta tggagaatat agacatgttt aaatcaaatc
     1861 atgagcgaag aatgcgttat tccattcgta aatttagtgt aggagtagct agtgtagctg
     1921 ttgccagtct tgttatggga agtgtggttc atgcgacaga gaacgaggga actacccaag
     1981 cacccacttc ttctaatagg ggaaatgaaa gtcaggcaga acaacgtaga gaactcgatt
     2041 tagaacgaga taaggtaaag aaagaggtca gggaatataa agaaaaaaaa gtgaaagagc
     2101 tctattcaaa atcaactaaa agtcgacata agaagactgt agatatagtt aacaagttgc
     2161 aaaacattaa taacgagtat ttgaataaaa taattcaatc aacctcaaca tacgaagaac
     2221 tacagaaact gatgatggag agtcaatcag aagtagataa agctgtgtct gaatttgaaa
     2281 aggacttatc ttcttcgtca agttcaggct cttccacgga accggaagct tcagatacag
     2341 cgaagccaaa caagccgaca gaactagaaa aaaaggtagc agaagctcag cagaaggttg
     2401 aagaagctga gaaaaaagcc aaggatcaaa aagaagaaga tcaccgtaac tacccaacca
     2461 ttacttacaa aacgcttgaa cttgaaattg ctgagttcga tgtgaaagtt aaagaagcgg
     2521 agcttgaact attaaaagtg aaagctaagg aatctcgaga cgagaaaaaa attaagcaag
     2581 cagaagcgga agttgagagt aaacaagctg aggctacaag gttaaaaaaa atcaagacag
     2641 atcgtaaaaa agcagaagaa gaagctaagt tgaaggaagc tgttgaaaag aatgcagcga
     2701 cttcagagca aggtaaacca aagaggcggg taaaacgagg agctcttgga gagcaagcaa
     2761 cacctgataa aaaagattat tttgaaaaag acttccgtcc agctttcaat aaaaaccagc
     2821 agatggtagc cattcaagaa tccttgaaca aactagatga tgaaacaaaa actgttccag
     2881 atggggctaa actcacagga gaagctggaa atgcctataa tgaggtcaga gattatgcaa
     2941 taaaagttgt ttctgaaaac aagaaacttc tatcacagac agcagtgaca atggatgaac
     3001 tggcaatgca attaaccaaa ttgaacgatg ccatgtctaa attgaaagaa gctaaagcga
     3061 aattggtacc agaggttaaa ccgcagccgg aaaatccaga gcatcaaaaa ccaacaactc
     3121 cagctccgga taccaaacca atccctcaac cagagggtaa gaaaccaagc gtaccagata
     3181 ttaatcagga aaaagaaaaa gctaagcttg ctgtagcaac ctacatgagc aagattttag
     3241 atgatataca aaaacatcat ctgcagaaag aaaaacatcg tcagattgtt gctcttatta
     3301 aggagcttga tgagtttaaa aagcaagctc tttctgaaat tgataatgta aataccaaag
     3361 tagaaattga aaatacagtc cacaagatat ttgcagacat ggatgcagtt gtgactaaat
     3421 tcaaaaaagg cttaactcag gacacaccaa aagaaccaga taa
//
LOCUS       DBEROU010000088         3212 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.088, whole genome shotgun sequence.
ACCESSION   DBEROU010000088 DBEROU010000000
VERSION     DBEROU010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.088"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            51..245
                     /locus_tag="ACL8CL_002100"
     CDS             51..245
                     /locus_tag="ACL8CL_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HGS1331261.1"
                     /translation="MKGKEYENRTVSFAISSKGEKMKVENISYRVDHRILFDNISFDT
                     SSSGVTLITGKNGTGKSTLL"
     gene            complement(351..520)
                     /locus_tag="ACL8CL_002101"
                     /pseudo
     CDS             complement(351..520)
                     /locus_tag="ACL8CL_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558122.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(598..1908)
                     /locus_tag="ACL8CL_002102"
     CDS             complement(598..1908)
                     /locus_tag="ACL8CL_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HGS1331262.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTHPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2145..3017
                     /gene="nadC"
                     /locus_tag="ACL8CL_002103"
     CDS             2145..3017
                     /gene="nadC"
                     /locus_tag="ACL8CL_002103"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HGS1331263.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAILLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          995 a          528 c          725 g          964 t
ORIGIN      
        1 aatcatttaa aaaaatttta tttttttatt tttcattacg aataatatag atgaagggga
       61 aagagtatga aaacagaact gtttcttttg ctattagttc aaaaggagaa aaaatgaaag
      121 tagaaaatat ttcgtatagg gtggatcatc gtatattgtt tgataatatt tcttttgata
      181 cttcgagttc aggcgtgaca ttaattactg gtaaaaatgg tacaggaaag tcaactttac
      241 tatagtagat tgaaactaga atagtacacc tctacttcta aaatattgtt agaaatcgat
      301 ttgactgtcc tgttcttatt tcattttact acatcttaaa ttgagtatga agaaagtgcg
      361 ctcccatgtc ctgggaacgc actttcttca tatttttcat attcttgaat ccatcgataa
      421 agactattgg gatgaatttt taaagttgaa ctaatcattt ttacaggatg aggtttacag
      481 cagagagttt gaaggcttta tcaaaggttt ttcttggcat aatgactttt cctcgtttcc
      541 acttaatttt gtgtctactt tattatacca agtccaccct taagttagat aataaatcta
      601 acttaaggaa gctaaaagga tgagaatcca ggtggtcaag agtcccaaac ttaagctgat
      661 ggggacaccc agaataattt gctttttgaa ggcaaggccg cgttcctcta tattgggaag
      721 tgagagttga atgagagaac cagctgatga aaagggtgag atattagtag atagagcgcc
      781 aataacggtg gctgttgtga gtaagtgaat atcaatctga ggattttgag cactgatgat
      841 agcaatgatg ggaaagaggg ctggagctac aacggatagg gtggaactaa agagtgacat
      901 cactccggct atcacacaaa agaacagagg taaccagaaa tgaggaatgg ttgttgtcat
      961 gaggtgccct atcagtgtga ctaaacctga cttgaccgct agagacatta gtaagctcat
     1021 gccgcagagc atgataattg tagcccaggg aaccttagct aaaatggctt cttgcttccc
     1081 taatttgagc cttaaggcga ggcagaccat gagtattgag acaaagccaa tatcaaatgt
     1141 tttttgataa gtagctatcc aggcgatgtt tgggaaaatg agatgcaaca agggaaaaag
     1201 ccaaaccaaa accatgctgc tgatcatgag caaggtggtt tgtctttgaa ccttgctgag
     1261 gagtggtggt tggtcaatag tcaaggatga gtttgttctt cccttactat agtgactgta
     1321 acaggataat aaaagcaaga cgatgagtgg gtagataatg ctgacgataa agatatgatt
     1381 gccaagtgaa aaagcttgct cttcccatcc catttgctta aacaggcctt gaaagacaat
     1441 gcctgagcta ctggttatca aattagcccc tcctgaagct ccccaattga cggcttgagc
     1501 tccaatcaaa gggtgtttgt ccgctttttg acagagggta atcgctagag gacagcaaac
     1561 ggccatagta gtgaaaaatc cagcacctaa agcagacaaa agggttgcca tcaggtataa
     1621 aatcatgtag agggcgttag ggtgggtgcg tgtgcggtag agaatgtgtt gagccaaaac
     1681 atcaagagta ccgttagttg ttgcaacgtt ataaaagaga gagacgctaa aaatggtaaa
     1741 aaagagtgag gttggccaaa aatgaagaag ttctttgggg cttaatccca tgagagtggt
     1801 tgcgatgagg taagaaaaag caatagccag caggccaata ttgattttgg tgcggtaacc
     1861 aattccaatg gctagagcaa tggcgctaat cattattaaa tgaatcattg aattgtcctt
     1921 tcagttagaa tatggaaata ggttggcaat tttggcgctc aaaagaccac ctgtttccta
     1981 ttggtacttg actattatac acaaaaaaaa tacaattgtc ttgacaattg tattgacact
     2041 tgttagaatt aaaaatgagg tattcaggct tgtgatttcc tatggaagtt aatagtgatt
     2101 gcctctaatg cttacaagtg atattaaaaa tagaggacct agtgatgtca atcatttcaa
     2161 ctgatttaac cccttttcaa atagatgata cattgaaagc agccttgcga gaagatgttc
     2221 attccgaaga ttacagtacc aatgccattt ttgatcatca tggccaagcc aaggtgtcgc
     2281 tttttgccaa ggaagctggt gttttagcgg ggctaaccgt ttttcaaagg gtttttaccc
     2341 tatttgatgc cgaggtgacc ttccagaatc ctcatcaatt taaggatggg gatcgtttga
     2401 ctagtggcga tttggtttta gaaatcatag gctcggtgag aagtctctta acatgtgaac
     2461 gcgttgcctt gaatttttta caacatttat cagggattgc ttcgatgaca gctgcttatg
     2521 tagaagcctt aggcgatgat tgcattaagg tatttgatac tcgaaaaact actcctaatt
     2581 tacgtctttt tgagaaatat gccgtgagag ttggcggtgg ctataatcat cgctttaatt
     2641 tatcagatgc tatcctgcta aaagacaatc acattgcggc agtaggtagt gttcaaaagg
     2701 caattgctca agcgcgtgcc tatgcccctt ttgtgaaaat ggtcgaggtg gaagtggaaa
     2761 gccttgctgc tgccgaagaa gctgcggcgg cgggtgctga tattatcatg ttggataata
     2821 tgtcattgga acagattgaa caggccatta ccctaattgc aggacgttct cggattgaat
     2881 gttctggaaa tattgatatg accactatta gccgttttcg tggtttagcg attgattacg
     2941 tctccagtgg tagtttaacc catagtgcta agagtcttga tttttccatg aaggggttaa
     3001 cctaccttga tgtctaagtt gtaaaataaa ctaacttttt aaaggatgtc tttcctctag
     3061 aacgagtttt atgtcagata gtttaaacgc ctcttcaaat atagtaaaat gaaccaaaaa
     3121 tagtacacaa tgtggtataa tctttttatg gcatattcaa tagattttcg taaaaaagtt
     3181 ctctcttatt gtgagcgaac aggtagtata ac
//
LOCUS       DBEROU010000089         2974 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.089, whole genome shotgun sequence.
ACCESSION   DBEROU010000089 DBEROU010000000
VERSION     DBEROU010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.089"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(94..1380)
                     /locus_tag="ACL8CL_002104"
     CDS             complement(94..1380)
                     /locus_tag="ACL8CL_002104"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HGS1331264.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGIGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGARNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(1611..1847)
                     /gene="comW"
                     /locus_tag="ACL8CL_002105"
     CDS             complement(1611..1847)
                     /gene="comW"
                     /locus_tag="ACL8CL_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma(X)-activator ComW"
                     /protein_id="HGS1331265.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
     gene            2107..2922
                     /locus_tag="ACL8CL_002106"
                     /pseudo
     CDS             2107..2922
                     /locus_tag="ACL8CL_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259226.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1031 a          652 c          420 g          871 t
ORIGIN      
        1 aaagtttagg atatttgtta caacaagtta ggaggtcttc ttgtaactgt ctatagtata
       61 cccgacctat cttaaacaaa tcttaaaaat ctcttaggac caaacacttt ctaaaatatt
      121 tgtttgttca cgaccaggac ctactgagaa agtagaaata cgaacgccaa ccaattcact
      181 cacacgacga acatagttac gcgcattctc aggaagatct tccaaattgc gagctccggt
      241 aatatcttct gaccaacctg gcaactcttc atagataggc ttgcaacgtt tcaattgctc
      301 aagactagct ggatagtagt caatacgttg accgtcaaga tcataggcca cacaaatttt
      361 cacagtatcc aagccgctca aaacatcaat agagttcaaa gaaaggttag taataccaga
      421 aacacgacgg ctatgacgca tcacaactga gtcaaaccaa cctacacgac gtggacgacc
      481 agttgttgta ccatattcat gacccacttc acggatacgt tctcccactt catcaaacaa
      541 ctcagttggg aaaggaccat ctcctacacg actcgtataa gctttacata cacctacaac
      601 cttgtcaatc ttgcttggac cgataccaga accaattgtc acaccaccag ctacagggtt
      661 tgatgacgta acaaatggat aagtaccttg atcgatatct agcataacac cttgtgcacc
      721 ttcaaaaagc acacgtttgc cattatcaag cgcatcattc aagataacag atgtatctat
      781 cacgtatttc ttgatttgtt gaccatattc gtaatattct tcaaaaatat catcgaaaac
      841 aatcgcttta ctgtcataca atttttcaaa aagacgattc ttttcagcaa ggttacgttc
      901 taaacgctca cggaaaatat ctttatctaa aagatctgca atacgaattc caacacgagc
      961 agccttgtcc atataagctg gaccaattcc cttaattgtc gtaccaatct tattgtcgcc
     1021 cttagcttct tcttgcaagc gatccaactc gatatgataa ggcaaaataa catgcgcacg
     1081 atcagaaata cgcaagttat cagttgttac accttcctca tgaagatagc tcaactcttt
     1141 tacaagagat ttaggattta caaccatacc attcccaatg acagatattt tttcagggaa
     1201 gaaaatccca gatggaatca agtgcaactt aaatttctta ccgtcaatca caatcgtgtg
     1261 accagcatta tcaccacctt ggtaacgtgc aattacttct gcattcgctg aaaggaagtc
     1321 tgtaatcttc cctttacctt catcacccca ttgggtacct acaacaacaa ctgaagtcat
     1381 aattttgtct gagccctcag gctcttcctt tctcacatac atggcaggac tctcacctgc
     1441 aattatatct tacaatttat tataataaaa aatcgccttt ttatcaagaa gaaacaatag
     1501 aaagatttgc tatttccaac tattaaaaaa tgatttagaa aaattactag ctatttacta
     1561 ttatctttcc ataaaagagt aaattagttc ggaaatttac taaaattacc tcaacaagaa
     1621 ataaaacccc gattcattac caatttttca agatacaaac gataagcaac acgataatgg
     1681 taaacgataa aatccctacg acaaccaatg ccatatctca ctaaataata aattaaaaat
     1741 ttaaaatgaa catgttccca gtcaaaatta tcaccaaatg taggaccata ctcttcttca
     1801 atactatcat agaaattagc catctgctca taaatttttt gtaacataat caaatactcc
     1861 ttttcttttt tataaactta ttctaacaaa aaattttaca cattcactat caattcctga
     1921 attgttaaaa taccagcctc aacaagataa aaataggaaa agttgacaga atagaaacaa
     1981 atttgctacc taaatttcaa acgattaaag ttctaaaaag accgccttaa aacttttggg
     2041 taaatcctat tagaactata atatttttat ataggtatta tggttctgat tttctagtat
     2101 aaaaaaattc aaactagaat tcttcttata aaagtaaaag gccgttttaa gtgctatcat
     2161 tgctcaaaaa tggaggtcgc tgagacttct atcgtcaaga agaatcatca aattccttgt
     2221 atcatcaacc aaaagattgc tcagaagcta attgaaaaga cttctatgac cgacattgat
     2281 catcagttgt ctatttcaat ttcaacttca actgtcattc gcaagatcaa tgattttcac
     2341 tttgagcatg atttttcgcg tcttcctgag attatgtcct aggacgttga aacagtccgg
     2401 ggagtgactg tttcaatcgg gagatggaga tgagctttat tgctcaagac tttgataagc
     2461 tcaatatcat aactgttctt gagagcagaa cacaagccat catccgaaat cccatgaata
     2521 caaggctatc aagcgatact gaaagctcat tcaacaagat agtcagaaac tgagtgataa
     2581 acgattttat cgccctactt ttcgtatgca cttgactaat aaagaaattc tagataaact
     2641 tttgagctat tccgaagact tgaaacacca ctataatctc tatcagctct tgcttttcca
     2701 cctctttttc agactctgtt taaaaccttc ctcaaagata aagagaaaat cgtcaacgcc
     2761 cttcaattac cttattccaa cgccaaactg gaagccacca ataatctcat caaacttatc
     2821 aagcacaatg cctttggttt taggaacttt gaaaacttca aaaaagaaag gacgaaattt
     2881 gtcctttcta gattttcgct ttcttcaacc cactacagtt gacaaagaac cgaaaaatgt
     2941 ctcgcctcta atatggtcaa ttgaaacaag aaca
//
LOCUS       DBEROU010000090         2926 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.090, whole genome shotgun sequence.
ACCESSION   DBEROU010000090 DBEROU010000000
VERSION     DBEROU010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.090"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(112..570)
                     /locus_tag="ACL8CL_002107"
     CDS             complement(112..570)
                     /locus_tag="ACL8CL_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HGS1331266.1"
                     /translation="MKKQVFHDAAAGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWAAIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFAIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(567..1007)
                     /locus_tag="ACL8CL_002108"
     CDS             complement(567..1007)
                     /locus_tag="ACL8CL_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HGS1331267.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKIDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVLKLLPTNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            1591..2457
                     /locus_tag="ACL8CL_002109"
     CDS             1591..2457
                     /locus_tag="ACL8CL_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000537001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HGS1331268.1"
                     /translation="MGTLLATRLKNRRKELKMSQRELAEGICKQGQISRLESGEFTPG
                     ADFLHALAKKLKVSMDYFFDEQVVEKVEELSEFKKLAQTFITNRNYESLKYIYELENV
                     KAHRLSLADKIYMEWIKSLIDFYFYGQKEDAIVRLEGVLSQLSVSDLNYLQVSNTLFN
                     FYYDIGDLGRFNKIREKLEYRVNQLNLSTIEELELSIKFNYNVCRYLWLQKNIEEAIT
                     KITATIKQCKAYRTNYLLADLYLLMGNVSENFSSKSSVKEYFETAHFLYKLEENMSMA
                     LKVEHYIADITE"
     gene            2607..2741
                     /locus_tag="ACL8CL_002110"
     CDS             2607..2741
                     /locus_tag="ACL8CL_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331269.1"
                     /translation="MRSLFKKIVAVLVIGLILLGIARTPQVHKIARGIDPGPANGQAL
                     "
BASE COUNT          963 a          441 c          619 g          903 t
ORIGIN      
        1 gactgtcctg atcgatttat cctgttctta tttcatttca ctatagtaag gtgcagcagg
       61 ataaagaaaa accgagaagt ttctttctcg gatttgctta ttatgaggtt cctattttct
      121 atgctccaag agttgattga tgtgatctac ttttttagcc tctcttttat agagaatagc
      181 ccagatgatg agatagacaa tcgcaaactc gataatgagc tggaggtaga aaatccagtg
      241 gaaggggaac caaccagcaa gagttgctag tgggacaaag ccagccagca taaggaagaa
      301 atgagtcaga gtggcacgaa gcatgctcca gtcacggctg aataagcggt tgccaaagtt
      361 gaagagcata ccgatggctg cccagataag tgtgcagtag agcaagacca gggcaccgtg
      421 aacctgatgc tgatccatca cttggccgat gagagagtag ggatttagtg gtgcgtaggt
      481 atttggtgca taaatgagtg aaaagaggat ggagaggatg aggccgataa gaacaccggc
      541 agctgcatcg tgaaatactt gttttttcat agttctaatt tctccttgat ggtttttaga
      601 taacggcgtg aagagtaggt gaagctttcg tttttcaaga aaatttctac cagaccgttt
      661 ggcgtgagct tgaggtgaga gatggaatcg atattgatga tttctgattg ggaaatttgg
      721 ataaaattgg ttggcaagag tttaagaacc tgatagagtc gcaaatcaat gctgtaggtc
      781 tgactcgcgg tttctgctag aaccttccga ttctcgatat agaagcgctg aatcttgcca
      841 atctcaacta gatagacctg atcatcgatt ttccctttga ttttttctct ttggtccaga
      901 ttttctgcga actcgatgac tttctgaact ttatctgtcg gctgaggtgc ttggacaatc
      961 agcttttcct cctcgtaagt ctcactaatc tgtaattcta ctttcataaa tttctctcct
     1021 tgtttttcat acaaaattaa tctcacttct tgtacacctt tattaatact cttcaaaaat
     1081 ctcttcaaac cacgtcagcg tcgtcttgcc gtaggatagt tgttgacttt gtcagtttta
     1141 tctacaacct caaaacagtg ttttgagctg tctgcgccta gctttctagt ttgctctttg
     1201 atttttattg agtataccac tattttactc cctctggcaa ggtactttgt ctatgtggag
     1261 ggattgggct cctatgtggt ggagcttttc tgttctttct gaaatatggt ataatagcac
     1321 taatcaattt ctaggaaaat agatacagaa aggggctgaa agaactaggg gaacagttgt
     1381 aattagaagg tgataggtat gaaaagtggc gttgaagctg gataagttta taaagagttt
     1441 gaatgaagaa atgaaaagta aataagttga tgatgaattg caaaaataga ttttatatac
     1501 aatttgtatc tttcgaatca ttgatttgaa ttttagattt aatcatgtta caattaaaac
     1561 tgaaatcaaa tagaagagga aaatgcaaac gtgggcacac tattagcaac aagattaaaa
     1621 aatagacgga aagaattaaa aatgtctcag cgagaactag ctgaaggtat ctgtaaacag
     1681 gggcaaatca gtcgattaga gagcggagag tttacccctg gagcggattt cctgcatgcc
     1741 cttgctaaga agttaaaagt cagtatggat tatttttttg atgagcaggt tgtagagaaa
     1801 gttgaggagt tatcggaatt taagaaatta gcacagacat ttattacaaa tcgcaattat
     1861 gagtctttga aatatatata tgaattggag aatgtaaaag ctcaccgcct atctttagca
     1921 gataaaatct acatggaatg gataaaatct ctgatagatt tttattttta cggacagaaa
     1981 gaagatgcga ttgtacgatt agagggggta ttatcacagt taagtgtatc tgatttgaac
     2041 tatctccaag tttcaaacac cctattcaat ttttactatg atattggaga tttaggtcgt
     2101 ttcaataaaa ttcgtgaaaa actagagtat cgagtaaatc aactcaattt gagtacaatc
     2161 gaagagttag agctatctat taagtttaac tacaacgttt gtcgttatct atggttacag
     2221 aaaaatattg aagaagctat tactaaaatt acagctacta taaaacaatg taaggcatac
     2281 agaacgaatt atcttttggc tgatttgtac ttattgatgg gaaacgtaag tgaaaatttc
     2341 tcttctaaaa gttcagtgaa agaatatttt gagacagctc atttccttta caaattagag
     2401 gaaaacatgt caatggcttt gaaagttgag cattacattg cagatataac agaataatga
     2461 atacggtgtt caagtgagca tcgtttttca aacccagttt agatacaaaa aagtatcaaa
     2521 caaaaaaaaa taaaaaaaca ttataaaaat acttgacaag ctaattttga agtgataaaa
     2581 taaatgcaag aaaaaaggag aggtatatga gaagtttatt taagaaaata gttgctgtat
     2641 tagttattgg attaattctg ttagggattg cgagaacacc ccaagttcat aagatagcta
     2701 gagggattga tccaggtcca gcgaatgggc aagctctcta actgttcaga ctaatctata
     2761 acgacattat gaggtttgat aagatttctc ctcaccggat aaaaatctta aattttataa
     2821 tagagggaga aattaaattg aagtttaaga agaagagctt aaagcctaag aaaattcgtt
     2881 ttgtactagg ttacaatggc ggtgataaat aagtacaagt atgtat
//
LOCUS       DBEROU010000091         2801 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.091, whole genome shotgun sequence.
ACCESSION   DBEROU010000091 DBEROU010000000
VERSION     DBEROU010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.091"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            13..618
                     /locus_tag="ACL8CL_002111"
                     /pseudo
     CDS             13..618
                     /locus_tag="ACL8CL_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            598..723
                     /locus_tag="ACL8CL_002112"
                     /pseudo
     CDS             598..723
                     /locus_tag="ACL8CL_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(695..2170)
                     /locus_tag="ACL8CL_002113"
     CDS             complement(695..2170)
                     /locus_tag="ACL8CL_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HGS1331270.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTDKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSILLIGEQMNSEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEKVIELSEYARREINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSERKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
BASE COUNT          848 a          616 c          500 g          837 t
ORIGIN      
        1 ggaggtactc ccatgcctta tgtgggcatt gatgtcgcta aaaataaaca cgatgttaca
       61 gccttgaatg ttcaaggaaa aactgttctt aaaccactca ctttttcaaa taataaagct
      121 ggttttgaac tcttagatct gtctcttcga cagctcaacc aagactgtct catcgctctt
      181 gaagatactg gacattatgc ctttaatcta ctgaacttcc tgcatgaaca aggatataaa
      241 gtttacacct acaatccttt gctcatcaag aaatttgcca aatcattatc acttcgcaag
      301 actaaaacgg ataagaaaga cgctcatggc atcgctctta aacttctttc tgaccccaat
      361 cgtgaacaat ttcaacacga taatcggcaa gtagaactaa aaatactggc tagacatatt
      421 catcgtctca agaaaaaaca gtctgattgg aaagtacaat acactcgttg tcttgatatc
      481 atctttcctg agttggataa aatcgttgga aagcattcag aatataccta ccaactcttg
      541 acacgctacc ctaatcctca gaaaaggctt gaggcaggat ttgataagct gatagaaatt
      601 aagcgattga ccgcttctaa ttcaagggct ttttgccgtg ctctaaaatc atcgccaaat
      661 catcaaattg aaataagaaa tagtttagat aaaactattg acttttctta tagtttgtct
      721 ttctcttaat aacgttgata tgattgacct ctggatcttc cgtcccttgg agggagcaac
      781 cacgttcctt ggcgaattgg atatagtcga caatttctcg tgtaatgcgt tcaccaggag
      841 ccaagatagg aatacctgga gggtaacaca taacaaattc tccacagacc tgtccaacag
      901 aatcatccaa agttaaactt tttctttctg aatagaaggc ttcttgcgga gacagcacta
      961 actcgggctg aatatattct cctgctatca aatcttttcc atctcttgaa tagagtctct
     1021 taatatcagc cagagcacca accaagcgct cgatgtcttg gatgcggtcg ccgatggaaa
     1081 tataggccaa gatattgccg atatcaccaa actcgatctg aatgtcgtat tcgtctcgca
     1141 agaggtcata aacctcgata cctgttaagc caataccctg agtgtaaact gacagcttag
     1201 ttacgtcaaa atcgcaaacc gaaacaccgt ctattaactc ttttgagtag gcatagtagc
     1261 caccgatagc attgatttca cggcgggcat actcagatag ctcaatgact ttctcaaacg
     1321 actctttacc acgaagggcc aagttgcgac gtgaaatatc caaactagcc atcaacaagt
     1381 aagaggcaga tgtagactgg gtcaggttaa ttatctgacg aacgtattca gaattcatct
     1441 gctccccgat aagtaaaatg gagctttggg tcaaactccc accagactta tgcatggaaa
     1501 ctgctgccat atcagcccct gcatccatag cagaaattgg aagtttatca gtgaaatgca
     1561 aatgcgctcc gtgggcttca tctactaaaa ccatcatgcc agcttcatga gccatttctg
     1621 tcaacccctt taggtctgaa cagatgccgt agtaagtagg attgttgatt aggatagcct
     1681 tagcatctgg atggtccttt atggcctgtg ctactcggtc attttcaaga cctaaagcga
     1741 taccaatctt aggatctaca ctcatctcga tatagatggg aatggcacca catagaacca
     1801 acgcattgat agcagattta tggacatttc gtggcagaat aatcttatct cctgccttgc
     1861 aggttgccag aatcatagtc tgcaccgatg aagttgttcc accaatcatt agaaaggcat
     1921 ggctagctcc aaaagcatct gcagccagct cctctgcatc acgaataatc gaaataggat
     1981 ggcctaaatt atccaagggt ttcatcgaat tgacatcaat gcctacacat ttttctccta
     2041 agagttcgac aagttctgga tttccccgtc cacgcttgtg acctggaaca tcaaagggaa
     2101 caatcctttt cttgcgtaac ttcaccaagg cctcataaat tggggcttgg ttttgatcta
     2161 actctttcaa aacatactcc tttcaatatt tttaggacct tatgaaaact aaggaactaa
     2221 aggttgactc atgaaaaaag agcaggtttt cacctgctct gcaatcttct gacgtgttta
     2281 ttttggtttc tgttgagtat gtctgttccc gatgtttcct aactctctag tagcaaatat
     2341 tacgtacttt attgatcgtt tcacaagatg acgtgtgctt tcaccacact aaaaatttat
     2401 gaattagaac aagtgtccta acatcaactt ataaaagtag gcttttaacc ctatcaataa
     2461 tgtggctttt caaccacgct tcggcattca accctgcctg tccgtaggca ttttttactc
     2521 gggtttatat ttgctagaaa acatcatctc attttctaag ctttattact atatcatgtt
     2581 tttaagaact tgtaaagtat tttttgataa aaaataaaaa tatagtagat tgaaactaga
     2641 atagtacacc tctgcttcta aaacattgtt agaaatagat tttactgtcc tgatcgattt
     2701 gttctgttct tgtttcattt tactatatgt tcgtaaatga aagttctata atgaagttag
     2761 ccacccagcc ctcctaaaaa ttagatggtt caactaaccg a
//
LOCUS       DBEROU010000092         2397 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.092, whole genome shotgun sequence.
ACCESSION   DBEROU010000092 DBEROU010000000
VERSION     DBEROU010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.092"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..506)
                     /locus_tag="ACL8CL_002114"
     CDS             complement(<1..506)
                     /locus_tag="ACL8CL_002114"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1331271.1"
                     /translation="MKKVKLGEVATFINGYAFKPQDWSSEGKEIIRIQNLTKTSTEIN
                     YYSGTIDKKYIVEAGDILISWSGTLGVFQWRGRSAVLNQHIFKVVFDKIDIDKSYFKY
                     VVEKGLQDAVKHTHGSTMKHLTKKYFDNIMVSYTNLGEQQRIASELDLLSKLILRRQE
                     QLEELNLLV"
     gene            complement(540..1505)
                     /gene="xerA"
                     /locus_tag="ACL8CL_002115"
     CDS             complement(540..1505)
                     /gene="xerA"
                     /locus_tag="ACL8CL_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HGS1331272.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKAQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            complement(1623..2036)
                     /locus_tag="ACL8CL_002116"
     CDS             complement(1623..2036)
                     /locus_tag="ACL8CL_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HGS1331273.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSSSALNMIVNLPEQ
                     FVFGKSLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWVSEHSVREKVKK"
     gene            complement(2033..2269)
                     /locus_tag="ACL8CL_002117"
     CDS             complement(2033..2269)
                     /locus_tag="ACL8CL_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HGS1331274.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
BASE COUNT          710 a          478 c          341 g          868 t
ORIGIN      
        1 acaagtagat taagctcttc aagttgttct tgtctcctta aaatcagctt gcttaacaaa
       61 tccagctcac ttgcaattct ttgttgctct cccaaatttg tatatgaaac cataatatta
      121 tcaaaatatt ttttagttaa atgtttcatt gtggaaccat gagtatgttt tacagcatct
      181 tgtaaacctt tttcaacaac atatttaaaa tacgatttat ctatatctat cttatcaaaa
      241 accactttaa aaatgtgctg atttaacact gcggatcttc cacgccactg aaaaacccct
      301 aatgtacccg accacgaaat aagaatatca ccagcttcaa caatgtattt tttatcaatc
      361 gtacccgaat aataatttat ctctgttgaa gttttagtca gattctgtat tctgataatc
      421 tctttccctt cagatgacca atcttgaggt ttaaaagcat agccattgat aaaagtagcc
      481 acttcaccta atttcacttt cttcatgcaa ccacctcctc ggaatatcgg gatttgcttt
      541 taagagatga atttttggtg tgaatgcttg acattatttt gattgaccat ggcataggcc
      601 agggttgtgt caatcttgct atgacctagc agtttttgga cttgttcgat aggcatacct
      661 ttatcaattg ccttagtcgc taaagttctt ctgaacttat gtgggtgaac cttttgtatg
      721 ccgagtttgt ctcctaactt tcttaagcga atctctacac cagcaattcc aagtctctgg
      781 gcttttccaa ctagcgttac aaaaagagca gggtgactat cttttctgtc gttaagataa
      841 tttcttaaat gaattttcgt ccgagcgtca aaatatactg gtctctcctt ctttccttta
      901 ccaaagacaa cacactctct gttttcaaaa tcaatatctg aacgattcaa ctgtacaagc
      961 tcccctacac gcatacctgt cgatgctagt aggtctatta ttgccaaatc tctcaaattt
     1021 tcacagttat cacgcataat ttccaaatgt tcatcagtat aagtttcctt cacattttgc
     1081 tcagtcttaa ttttctttat ccgtcgaatg ggatttttaa tgatatattc ctcttgctca
     1141 agccaagcaa aaaaagaaga caatatacgc ctaatattgt ctaaatttgc cttactacaa
     1201 tccttttcag actggtaatt tgctaaataa ctccttaaat catctgttgt gagttgtgtc
     1261 acagactctt taatagcatt aaacaagttt tcaatcgtcc tctggtaata acgaattgtt
     1321 tttgagctac agccctctac ctgcttggcg gtaataaata gttgcaacag gttattattc
     1381 gaatacttct gctgcttttt tccgtctact agatgatgtt ctaaactctc tctcaacgct
     1441 agtaattgtt cattatttaa aagaggtaac atttcttgca tgataattgt aacttttgat
     1501 tccataattt ctcactttct tattcatcaa taaaaagaga caatatcagt ttctgcatta
     1561 cagaattcta atattgtctg ttcatttatt ttcttgtgct cattcaaatc cagcatacta
     1621 ggttactttt tgaccttttc tctaacagaa tgttcagaaa cccatttgga gtagcttgtc
     1681 attttttcat cagttaaagc ttctactgcg atggttacac acatttttac tgcttcttct
     1741 accgttacag aaaaacgata gccgtttaat tgtaaaaatt tgaccaaaac gaagaaagca
     1801 gttcttttat tagcattagc aaaaacatgc ttctttatca attggacaaa tagtatcgtt
     1861 gctttatcaa aaattgttgg ataaagagac ttcccaaaga caaattgttc tggtaagttc
     1921 acaatcatat ttaaggcaga agaactaact gtttgaattt tctcatttgg agaataccgt
     1981 tgaattgcta aagcatttat tttttcaatt tgcttttctg tcagatagat tgtcattttt
     2041 ccaccaaagc tttgaaaaca tcgtcgtatt cgtcaaatat tttatctaaa tttaagtcaa
     2101 aggcatcatc agatacataa gaaacctgct gccccaattc ttcaggagta aagataatct
     2161 caccacttgg taacaatttc gcttcgtatt taacaccact tggaatatta aattcacttg
     2221 gaatcgtaat ggtgattgaa tttccttgtt ttcttgtttt tactaccatc tgatttcctc
     2281 ctttgaagta ttataacaaa tcattaccgt aattacaatg gcagaatatc agccaaaata
     2341 ctcctgcatc agagatttct tcaaagtttc aagttcttcc agagattttt ggattgc
//
LOCUS       DBEROU010000093         2267 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.093, whole genome shotgun sequence.
ACCESSION   DBEROU010000093 DBEROU010000000
VERSION     DBEROU010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.093"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..2137)
                     /locus_tag="ACL8CL_002118"
     CDS             complement(<1..2137)
                     /locus_tag="ACL8CL_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078464109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HGS1331275.1"
                     /translation="MFFRRQKGEYRETDRVTRFKLIKSGKHWLRASTSLFGLFKVMRG
                     GVDTTQVMTETVEDKVSHSITGLDILKGIVAAGAVISGTVATQTKVFTNESAVLEKTV
                     EKTDALATNDTVVLGTISTSNSASSTSLSASESASTSASESASTSASTSASTSASESA
                     STSASISISASSTVVGSQTAAATEATAKKVEEDRKKPASDYVASVTNVNLQSYAKRRK
                     RSVDSIEQLLASIKAAVFSGNTTVNGAPAINASLNIAKSETKVYTGKGVDSVYHVPIY
                     YELTVINDGSKLTFTYTVTYVNPTTKTLGNISNMSNGYSIYNSGTSIQTMLTLGSDLG
                     IPSGVKNSITDKNGRQVRSYNTSTMTTQGSGYTWGNGAQMNGFFAKQGYGLTSSWTVP
                     INGTDTSFTFTPYAARTDRIGINYFNGGGKVVESSTTSQSLSQSKSLSVSASQSASAS
                     ASTSASASASTSASASASTSASESASTSASASASTSASASASTSASSSASTSASASAS
                     TSASASASTSASSSASTSASASASTSASVSASTSASASASTSASVSASTSASASASTS
                     ASESASTSASASASTSASESASTSASASASTSASASASTSASESASTSASASASTSAS
                     ASASTSASASASTSASESASTSASASASTSASASASASTSASASASTSASASASTSAS
                     ASASISASESASTSASASASTSASASASTS"
BASE COUNT          576 a          485 c          547 g          659 t
ORIGIN      
        1 cactggttga cgctgaggct gaggcgctag tacttgctga agcggacgca ctcgttgatg
       61 ccgattcaga cgctgagata cttgctgagg ctgacgcact tgtgcttgct gaagccgacg
      121 cactggttga ggcggaagct gacgctgagg tacttgcgct tgctgaggcc gacgcactcg
      181 ttgatgctga ggctgaggcg ctggttgagg ccgattcaga agctgaggtg cttgctgagg
      241 ccgaggcgct ggttgatgct gaagctgaag cactggttga cgctgaagcc gaagcactgg
      301 ttgacgctga ttcagatgct gatgtgcttg ctgaagccga ggcacttgtc gatgctgagg
      361 ctgaagcact tgttgatgct gattcagatg ctgaggtact tgctgaagct gaggcactgg
      421 ttgatgcgga ttcagacgcg ctggtgcttg ctgaggccga cgcactggtg cttgctgaga
      481 ctgatgcact cgttgatgct gaagccgagg cgctggtgct tgctgagact gatgcactcg
      541 ttgatgctga agccgaggcg ctggtgcttg ctgaagacga cgcactggta cttgctgaag
      601 ctgacgcact ggttgacgct gaagctgagg cactggttga tgctgaagac gacgcactcg
      661 ttgatgctga agccgacgca ctcgttgatg ctgaggctga ggcagacgtt gatgcggatt
      721 cagacgcact tgttgatgct gaagctgagg cactcgttga tgctgaagcc gacgcacttg
      781 ttgatgctga agctgaggcg ctttgactag cacttactga gagtgactta gactgtgaaa
      841 gtgactgact ggtcgtgcta gattcaacta cctttcctcc accattgaag tagttaattc
      901 caattctatc tgttctagca gcgtaagggg taaatgtaaa ggatgtatcc gttccattaa
      961 ttggtacagt ccaagatgat gttaatccat atccttgctt agcaaagaaa ccattcattt
     1021 gggcaccatt tccccaagta tacccactac cctgcgtcgt cattgtagat gtattatagg
     1081 atcgaacctg tctaccattt ttgtcagtaa tggagttctt tacacctgaa gggataccaa
     1141 gatcactgcc aagggttaac attgtttgta ttgaagtacc tgaattatag atagaatatc
     1201 cattactcat atttgatata ttaccaagag tctttgttgt aggattcaca tacgtaaccg
     1261 tataggtaaa ggtcaatttt gaaccatcat ttatcactgt caattcatag taaattggaa
     1321 catgatatac cgaatctaca cctttacctg tataaacttt tgtctcactt ttagcaatgt
     1381 ttagacttgc attaattgca ggggcgccat ttacagtcgt attgccagaa aaaacagcag
     1441 cttttataga agccagcaat tgctcgatgg aatccactga acgcttgcgt cgcttagcat
     1501 aagattggag attgacattt gtaactgatg ctacataatc actagctggt ttcttacgat
     1561 cttcttcgac cttcttagca gttgcttctg tagcggcagc tgtttgtgat cctaccacag
     1621 tagatgatgc agagatactt atcgaagccg atgtacttgc tgattctgat gcacttgtac
     1681 tcgctgaggt cgaagcgctg gttgaggctg actcagatgc agatgtactt gccgactctg
     1741 aagctgacaa actagttgaa ctcgctgaat tacttgtaga tatcgtacct agaactactg
     1801 tatcatttgt tgccaaagca tccgttttct ctacagtttt ttcaagtact gccgactcat
     1861 ttgtaaatac cttcgtttga gttgcaacgg ttccacttat gacagctccc gcagcaacta
     1921 tccccttgag gatatcaagc ccagtaattg aatgacttac tttatcttct accgtttcgg
     1981 tcatgacctg agtagtatca acaccaccac gcatgacctt aaacaagcca aaaagagaag
     2041 tcgaggcccg tagccaatgc ttccctgatt tgattaattt gaaccgagtt actcggtctg
     2101 tttctctata ttcacccttc tgacgtctaa aaaatatttt gaaatttcct ttcgagtatt
     2161 attttcatgt atggatataa ataaattacc atactataat tatagaatag ggaacccaaa
     2221 atagcaagaa acttgaggta tttttgctaa aaaaaaaaag catttta
//
LOCUS       DBEROU010000094         2200 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.094, whole genome shotgun sequence.
ACCESSION   DBEROU010000094 DBEROU010000000
VERSION     DBEROU010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.094"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1299
                     /locus_tag="ACL8CL_002119"
     CDS             <1..1299
                     /locus_tag="ACL8CL_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1331276.1"
                     /translation="VRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDTEKGEKYI
                     NNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAISNYENSLN
                     KDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQRIIEAIESAL
                     EKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEK
                     QKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYIQRGKSPK
                     YSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNSTGLGTLG
                     RLAIYHENKNPYVWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEEKASGSTK
                     QKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            1356..2153
                     /gene="psrA"
                     /locus_tag="ACL8CL_002120"
     CDS             1356..2153
                     /gene="psrA"
                     /locus_tag="ACL8CL_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HGS1331277.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT          793 a          310 c          391 g          706 t
ORIGIN      
        1 gtgaggtttt ctacattggt tgaaattgtc agaggtggct ctccacgacc aatcaaagat
       61 tatcttactt ctgaagtaga tggaataaat tggataaaaa taggtgatac tgaaaagggt
      121 gaaaagtata taaataatgt taaagaaaaa atcaaaaaat cagggcttaa caaaactaga
      181 tttgtaaaaa aaggtacatt tttgttaact aattctatga gttttggtag accttatatt
      241 ttgaatgttg atggtgcaat acacgatgga tggttggcta tttcgaacta tgaaaactca
      301 ttaaataaag attacctatt ctatattctt tcatcaaatg tagtttattc tcaatttcta
      361 tctctaatta gtggagctgt tgtgaaaaac ttgaatagtg ataaagttgc ttctattctt
      421 atccctctcc ccccactatc cgaacaacaa cgcataatag aagcaatcga atcagcttta
      481 gaaaaagtag atgaatatgc tgaaagttat aatagactag aacagctaga taaagaattt
      541 ccagataaac taaaaaaatc tattcttcaa tatgctatgc aaggaaaatt agttgaacaa
      601 gacccaaatg atgaatcagt cgaagtttta cttgaaaaaa tacgagcaga aaaacaaaaa
      661 ctctttgaag aaggcaagat taaaaagaaa gatttggaca tttctattgt ttcccaagga
      721 gatgataact cttattatga ggaagtacct tgtgaaatac cagaaagttg ggaatgggtg
      781 aggttaaacg atataacctc ctacattcaa agagggaagt ctccaaaata ttcaaatatt
      841 cctatatacc ctgtgattgc tcagaaatgt aatcaatggt ctggcttttc tattgactta
      901 gcaagattta ttgatcccga aacagttcat agttaccaga aagaaagatt actaagagat
      961 ggtgatttaa tgtggaattc aacaggtcta ggtacgttag gacgattagc tatttaccat
     1021 gaaaataaaa atccttatgt ctgggctgtt gcagatagcc atgttacagt tataagagtc
     1081 ttatcgggag ttattaattg ccatttcatc tataattttc tttcctctcc tattgttcag
     1141 tcagttattg aagaaaaagc gtcgggtagt acaaaacaaa aagaattatt aacgaaaact
     1201 ataaaagaat atttaatccc cctcccaccc cttcctgaac aatccagaat cgttgataaa
     1261 atcgaacaat ttttcgccca tattgacgca ctaatttagt ataatagttt tacgtgcggt
     1321 ggaatttcta tcaaacataa gataggagtt ttacgttgaa agattttgaa ttacatttga
     1381 agaaagctgg tttagctgaa aatactgtgc gttcgtacct ttatggtgtt cgatttttct
     1441 tggagaatta cgaactcaaa atggaagatt tatttgaata caaaggatat ttattagaca
     1501 attttaaacc taaaactgtc aatttgcgac tacaaggagt aaataaatat ctggctttta
     1561 ttggtcatga tgatttgaaa ttaaaatttg tcaaagtaca gcaaaaaccg tttttagaag
     1621 atgtcattag ccatgctgat tacctttttc ttaaacgtag tttgaaaaaa gatggtattt
     1681 taaaatggca ttttgtcgtt tggtttttag gtgcaactgg ggctcgtgta agtgaactta
     1741 ttaaactaaa agtagagcat gtagaaatcg gttattttga catttactca aaaggtggta
     1801 aaattcgtag actgtacatt cctaaaaaat taagaaatag ttgtcttagt tggttagaat
     1861 cagaaaatcg ccgaagtggt tatttatttt tgaataaatt taacgaacca attacagcaa
     1921 gaggagttgc tcaacagtta aaaaattatg ctgataaata caaaatgaat cctaaagtaa
     1981 tttaccctca ttcttttagg catttatttg ctaagaattt tttagcgaag tataatgata
     2041 ttgccttgct tgcagatttg atgggacacg aaagtataga aactactcga atttatctaa
     2101 ggaaaacagc tactgaacaa caaaatattg tagataaaat tgttaattgg taaaaaataa
     2161 caggtggtca aactgactac ctgctatttt tgtgattatg
//
LOCUS       DBEROU010000095         1773 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.095, whole genome shotgun sequence.
ACCESSION   DBEROU010000095 DBEROU010000000
VERSION     DBEROU010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.095"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1274
                     /locus_tag="ACL8CL_002121"
     CDS             <1..1274
                     /locus_tag="ACL8CL_002121"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1331278.1"
                     /translation="KSIYWNFGQNKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQA
                     PSRARKLVSQNSVLFSTVRPYLKNIAVVRELKEYLIASTAFIVLDTLLNETYLKYYLL
                     SDNFINRVNNKSTGTSYPAINDYNFNLLLIALPPLSEQQRIVEAIESALEKVDEYAES
                     YNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLLEKIRAEKQKLFEEGKI
                     KKKDLDISIVSQGDDNSYYGNKDETTSYPIYEIPEAWRYIKFASLVNFRIGKTPPRSE
                     ATFWGTEIPWVSISDMPISGYVTNTRESISKLALKSKKIDISPKGTLLMSFKLSIGKV
                     AILDIPATHNEAIISIFPYANKENIIRDYLMIFLPLISTLGDSKDAIKGKTLNSTSIS
                     ELLIPISNHEEMKRIIFKVDLLFQKVSQLFE"
     gene            complement(1228..>1773)
                     /locus_tag="ACL8CL_002122"
     CDS             complement(1228..>1773)
                     /locus_tag="ACL8CL_002122"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1331279.1"
                     /translation="IPMNWVVIKIKDIFSMNTGLSYKKGDLSINNKGVRIIRGGNIKP
                     LEFSLLDNDYYIDTQFISSEQVYLKHNQLITPVATSLEHIGKFARIDKDYDGVVAGGF
                     IFQLTPFESSEIISKFLLFNLSSPLFYKQLKAITKLSGQALYNIPKTTLSELLIPLAP
                     FEEQELITQKVEKLFEKVNQL"
BASE COUNT          666 a          273 c          260 g          574 t
ORIGIN      
        1 taaaatcaat ttattggaat tttgggcaaa ataagccaga gaaatccttt aggtatatag
       61 atacgtctag tattgataga aaaaagaaca taatcaacta caaaaatcta caatatcttt
      121 cacctgaaca agcgccttcc cgtgctagaa aattagtttc gcagaatagt gtcttatttt
      181 caacagttag accatatcta aaaaatattg ctgtagttag agaacttaaa gagtatttga
      241 tagctagtac agcatttatt gttttggata ctttacttaa cgaaacatat ttgaaatatt
      301 atttattatc agataatttt atcaatcgtg ttaataataa atctactgga acaagttatc
      361 ctgcaatcaa tgattataat tttaatctat tattaattgc tctcccccca ctatccgaac
      421 aacaacgaat agtagaagca atcgaatcag ctttagaaaa agtagatgaa tatgctgaaa
      481 gttataatag actagaacag ctagataaag aatttccaga taaactaaaa aaatctattc
      541 ttcaatatgc tatgcaagga aaattagttg aacaagaccc aaatgatgaa tcagtcgaag
      601 ttttacttga aaaaatacga gcagaaaaac aaaaactctt tgaagaaggc aagattaaaa
      661 agaaagattt ggacatttct attgtttccc aaggagatga taactcttat tatgggaaca
      721 aagacgaaac aacctcttat cctatatatg aaatcccaga agcatggaga tatattaaat
      781 ttgctagcct agttaacttc agaataggaa aaactcctcc acgcagtgaa gctacttttt
      841 ggggaactga aataccttgg gtatctatat cagatatgcc tatatctggt tatgtaacta
      901 atacgagaga atctatttct aaattagcat taaaatcaaa gaaaatagac atttcaccta
      961 aaggaacttt attaatgagt tttaaattat ctattggaaa agttgccata ttggatattc
     1021 ctgccactca taatgaagcc attatatcga tattccccta tgctaataaa gaaaatatta
     1081 tcagagatta cttaatgata tttttgccac ttatctctac tttaggtgat tcaaaagatg
     1141 ctatcaaagg gaaaacatta aatagtacca gtatctccga attattaatc cctatttcta
     1201 atcatgaaga gatgaaaaga atcattttca aagttgattt actttttcaa aaagtttctc
     1261 aactttttga gtaataagtt cctgttcctc aaaaggagct aacggaatta atagctcgct
     1321 cagtgtagtt ttaggaatat tatataaagc ttgacctgat agtttagtta ttgctttcaa
     1381 ttgtttataa aataacggag aggacaagtt aaataataga aattttgaaa taatctctga
     1441 actttcgaat ggtgttaatt ggaaaataaa tccaccagcc acaacaccat catagtcttt
     1501 atcgattctt gcaaactttc caatatgttc taaagaggtt gctacaggtg ttattagctg
     1561 attatgtttt aaataaactt gctcagagga gatgaattgt gtatcaatgt agtaatcatt
     1621 atccaacaga gaaaattcta aaggcttaat attaccacca cgtataattc taacaccttt
     1681 attattaatg cttaaatcgc ccttcttgta agaaagacct gtattcattg aaaaaatatc
     1741 ttttattttt ataacaaccc aattcatagg tat
//
LOCUS       DBEROU010000096         1716 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.096, whole genome shotgun sequence.
ACCESSION   DBEROU010000096 DBEROU010000000
VERSION     DBEROU010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.096"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            142..574
                     /locus_tag="ACL8CL_002123"
                     /pseudo
     CDS             142..574
                     /locus_tag="ACL8CL_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
     gene            763..1062
                     /locus_tag="ACL8CL_002124"
     CDS             763..1062
                     /locus_tag="ACL8CL_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HGS1331280.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKASVDEANIKTLYSEKLIGISIPIK"
     gene            1323..1646
                     /gene="tnpB"
                     /locus_tag="ACL8CL_002125"
     CDS             1323..1646
                     /gene="tnpB"
                     /locus_tag="ACL8CL_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HGS1331281.1"
                     /translation="MMIQLSDLGQVHLVCGKTDMIQGIDSLAYLVKTHFELDPFSGQV
                     FLFCGGRKDRFKALYWDGQGFWILYKRFENGKLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPQI"
BASE COUNT          587 a          339 c          296 g          494 t
ORIGIN      
        1 gttatactac ctgttcgctc acaataagag agaacttttt tacgaaaatc tattgaatat
       61 gccataagaa gattatacca cattgtgtac tatttttggt tcatttcact ataataataa
      121 cgatactaga taatgaggaa aatgcaatgg caaacccaac tttcggagaa aaaaagcgaa
      181 tacagactat attgctcgct atggcgtata tgcagttatc cctaatcctg aacaaaaaca
      241 aattgttctt gttcaagaac caaatggtgc ttggttccta ccatgtggag aaattgaagc
      301 aggtgaaaat catcaggaag ccctaaagcg tgagttgatt gaagagcttg gtttcacagc
      361 agaaattggt acctattacg gacaagctga cgaatatttc tattctcgtc atcgtgatac
      421 ctactactac aatcctgcct acctctatga agcaactcct ttcaaagaag tacaaaagcc
      481 actagaaaac tttaatcata ttgcctggtt ccctattgac gaggctatca aaaaccttaa
      541 acgtggtagc cataaatggg ccattgaatc ttgaaaaaaa cagcataaga ttgactaata
      601 ctcttcgaaa ataaaattca aaccacatca gcgtcgcctt accatactca agtatagcct
      661 gcggctagct tcctagtttg ctcttttatt ttcattgagt ataaatcaag cgactttatt
      721 caaaaagtgc tataatagaa ttagaaaatc ggaggaaata ttatgaagct tatcaatacg
      781 acaaactcac actcgcaact tgtaaaaagt cagctagaaa gtaccgatgc aacccttgtt
      841 gaggtttatt ctgcagggaa tacagatgtt atttttaccc aagctccgct tcactatgaa
      901 atcctcatct caaacaaaca ccgtgctatt cgtgagcctg aaatcgaaac cattcaagaa
      961 tttttcttga aacgtaaaat tgataaggcc tctgttgatg aagccaatat caagacactc
     1021 tactcagaga aattaattgg aatttcaatc ccaatcaagt aagaaaattg agtaaaaact
     1081 cgatttgaga gaaagtccaa taagtctttc cataataaaa cgcatagtag caagagtttc
     1141 tacacccact attatacgtt tttatcattt tgaaatctgc tgctaatcaa tgaagaagca
     1201 aagatcaaac taggcagcta gttacaagtt acgtaagcgc gtcataacaa ggtatctatc
     1261 ctaattcccc aacttccagt atactagata aaaaataatt atcggaggaa aagtattgta
     1321 ctatgatgat tcaactaagt gatttaggtc aagttcacct tgtttgtggc aagacagata
     1381 tgatacaagg aatcgattca ctggcttatc tcgttaaaac ccactttgaa ttggatcctt
     1441 tctccggtca agtctttctc ttttgtggtg gacgtaaaga ccgctttaaa gccctttact
     1501 gggatggtca aggattttgg atactatata aacgctttga gaacggaaaa ctgacttggc
     1561 ccagtacaga aaaggatgtc aaagctctcg cacctgaaca agtagactgg cttatgaagg
     1621 gcttttctat cactccccaa atatagtgga tttaaactag aatagtacac ccctacttct
     1681 aaaacattgt tagaaatcga tttgactgtc ctgatc
//
LOCUS       DBEROU010000097         1679 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.097, whole genome shotgun sequence.
ACCESSION   DBEROU010000097 DBEROU010000000
VERSION     DBEROU010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.097"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            23..1024
                     /locus_tag="ACL8CL_002126"
     CDS             23..1024
                     /locus_tag="ACL8CL_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HGS1331282.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     misc_binding    1236..1465
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT          561 a          228 c          338 g          552 t
ORIGIN      
        1 atgttcgtaa tggaggactg aaatgaaaaa tataaatggg aaaaaggtaa ctatttatga
       61 tattgcacgt ttatcaggtt tttctccaaa aacagtttca cgagttatta atggtggagt
      121 aaatgttaaa gaagaaactt atcaagcgat tcaaaaagtt atagaggaat tgtcgtatat
      181 tccaaatgcg tatgcaaaaa atttaactaa aaaagaagct ataaatatat tgatttctgt
      241 aaaaaaaatc gactcttttc ctttaatttg gtttcatacg ctgttagata aagtgttacg
      301 gacgtgtaaa gaatttggtg ttaatgctat agttgagtac tttggagagg aggatacaat
      361 tagtaattca attatatcaa gcacaggtag cctagtagat ggtgttattg ttttttatga
      421 aagtgtagat gatatcagaa ttcagtattt aaagaagaac catatgcctt ttcttgtttt
      481 cggtgaatct caaacgtctg gagtagtcta tgtatctaat aataattttc aagctactta
      541 tgatatgatg aaagctgtaa ctgaagaaaa atttaaaaat atgtggttgc ttatgggagg
      601 agaatcccat gttaataagg atcgtgaaag aggtgtgcgt tcttttctga atgataaaaa
      661 ttactttatg gatttaaaag tcatttatgg cttatctaca attgattcag tttattctta
      721 tgcaatgcag cacttgacta cccaaaatta tccggacatt atttttgttt caggagatga
      781 aaaggttcag ggcttgattc gtgcgtgcta tgagaaagga attcttattc cagatgatat
      841 ttctatcatt ggatttgata atattcctat ttctcagtat tatactccag ctttgtctac
      901 aattgctccc aattatgtta aattggctaa agaaatgata gaaggtgttt tagcaattat
      961 taaaggggaa agtgtcacat ctgttgaagt ttctcctaaa tttgttagga gacagagttt
     1021 ctagtattgt ttaattcttg aaaatcctag tggaaaagat ggagtgttct aaaataagtt
     1081 ctgtttagcc tccattcagt ttctattgag ttggatccaa tttctaacaa gtgggacatg
     1141 ttcatgttcg atctaaggaa gacatggtca cattcgaatg atattttcaa aaaaatcgta
     1201 aaaaagtctt gacaaagaaa aaacaaagta ttaaaataag ttcaacaaat cagaaaagta
     1261 actatttttg atcttcaggg agcctgtggt ggttgtgaat aggtggttgg aagtagtgaa
     1321 agtgggctga ttttgaaaat gaatttgaaa caatgaaaat tcggtgcgca caccttacag
     1381 tgcagcttgt tgttaaacaa ggcagagatg taaagggata gtccctttat aattgaggtg
     1441 gcaccgcgtt accaacgccc tcacacggaa gtatattctg tgtgtgggct tttttctatc
     1501 cgtcgtttgg tttatctttt attagggcct taagtcgctt tgatgaactt gagttctatc
     1561 tacagctcct tgcctactac taaaagcaaa caaaaaggct ggactaatac gaaaaaagct
     1621 agtaatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc tgctcaaaa
//
LOCUS       DBEROU010000098         1529 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.098, whole genome shotgun sequence.
ACCESSION   DBEROU010000098 DBEROU010000000
VERSION     DBEROU010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.098"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(39..647)
                     /locus_tag="ACL8CL_002127"
     CDS             complement(39..647)
                     /locus_tag="ACL8CL_002127"
                     /inference="COORDINATES: protein motif:HMM:NF016726.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HGS1331283.1"
                     /translation="MQIKFIDKVSNLIMLNLLYVASVVTVIAIGSGESALIATLIKIV
                     RHEESYPYRDFANSFFKDYWKNLGAALISNLPILILLFSLFFLPYIPLPIYIISILRH
                     IGVIYIILHLIATTFLIPLIGRYNNTLKNSLHNSIMLAYKHFFIAVLIRIIEIIPVLL
                     FFILQNQLLVWITLMIFILPSITKYANAFLYNFIFSKYEKLN"
     gene            825..>1529
                     /locus_tag="ACL8CL_002128"
     CDS             825..>1529
                     /locus_tag="ACL8CL_002128"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HGS1331284.1"
                     /translation="MADYILVRDSEIVYETSSEIVSKVKVGEGTLNRFCKKIGYSGFQ
                     ELKLKMTKDILELESQKMSSDTFIDEIKNNYLSIVESTRKLIDGRQIELAIKLIREAN
                     QILMIGVGSSGNAAREFESSLLRIGIISKTVIDTHFQLMHTALLKDNDLIIAFSLSGS
                     TKEVEETLLNAKRKNVKIISITNYSSRNIAKLSDCVLLTSKKESYLEGGSLMAKASQL
                     FIIDVICTRLSLINYED"
BASE COUNT          602 a          159 c          256 g          512 t
ORIGIN      
        1 tttttataaa atcaagatgc aaggctatga gatgttctct agtttaattt ttcatacttc
       61 gagaatataa agttatataa aaatgcatta gcatattttg ttattgaggg aagtataaaa
      121 atcattaaag ttatccaaac tagtaattga ttctgtaata taaagaagag tagaactggg
      181 ataatttcaa taattctaat aagaacggca ataaaaaaat gtttataggc taacatgatt
      241 gaattgtgta gagaattttt taaagtgtta ttatatctac cgattaaagg aattagaaat
      301 gttgttgcta ttaaatgaag tatgatatat attactccaa tatgtcgtaa gatagaaatg
      361 atatatatag gtaatggtat gtagggtagg aagaacaaac tgaataataa gattaaaatt
      421 ggtaaattag atattaatgc tgctcctaaa tttttccaat aatctttaaa aaaactgttt
      481 gcgaagtctc tatagggata ggattcctcg tgtcgtacaa tttttattag agttgctatt
      541 agggcacttt cacctgatcc gatagcaata actgttacta ctgacgctac atagagtaga
      601 tttaacatta taagatttga aactttatca ataaatttta tctgcatctt taactcctgc
      661 tgtttttata cttataatat cacatcattt gagtttcatc aactttgaat acattaagaa
      721 agaatataac tatgaggaaa ggaaaaaatt attatgaata aatccaaaaa atataaagaa
      781 agaattcatt atgaatacga taatcttact aaatcagaga aaaaattgct gactatattt
      841 tagttagaga tagtgaaata gtttatgaaa cgtcttcaga aattgttagt aaagtaaaag
      901 ttggagaggg aactcttaat cgattttgca aaaagattgg atactctggt tttcaggaac
      961 taaaattaaa aatgacgaaa gatatattag agttagaatc tcaaaaaatg agtagtgaca
     1021 catttattga tgaaataaaa aataattatt tatctattgt ggaaagtaca agaaaattaa
     1081 ttgatggtag acaaatagaa ttagctataa aattaataag agaagcaaac cagattttaa
     1141 tgattggtgt gggttctagt ggaaatgcgg ctagagaatt tgagagtagt ttgttaagaa
     1201 ttggaataat aagtaagacg gtaattgata cccattttca actcatgcat actgcgttat
     1261 tgaaagataa tgatttgata atagcgttta gtttatctgg ttccacaaaa gaagttgagg
     1321 aaactttatt aaacgcaaaa agaaaaaatg ttaaaattat ttcaataaca aattattcgt
     1381 ccagaaatat agcaaaatta tctgactgcg ttcttttgac atcaaaaaaa gaatcttatt
     1441 tagagggtgg tagtttaatg gctaaggctt ctcaactatt cataattgat gtaatatgca
     1501 ctagactctc tttaataaat tatgaagat
//
LOCUS       DBEROU010000099         1364 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.099, whole genome shotgun sequence.
ACCESSION   DBEROU010000099 DBEROU010000000
VERSION     DBEROU010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.099"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..1242
                     /locus_tag="ACL8CL_002129"
     CDS             join(<1..332,332..1242)
                     /locus_tag="ACL8CL_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS1331285.1"
                     /translation="MVKLIDRYGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSIS
                     LDYALPNQGMLSFWPAQYKKNGYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENE
                     RLRTEVAYPKKVKRVRGKGRSPRARKAETVREMVSGGFRLDFLLETARLARSTYYYQL
                     KQLDGVDKDKEIKTEIQGIDNEHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLT
                     ARIRRKRKYSSYQGEIGKKAENLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPV
                     LDGFNSEIIAYHLSTSPNLEQVKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLE
                     SKEIQASMSRKGNSPDNGMMESFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNN
                     KRIKVKLKGLSSVQYRTKSFG"
BASE COUNT          486 a          225 c          288 g          365 t
ORIGIN      
        1 catggtgaaa ttaattgacc gttatggcat agaaatcgtt aaaaaaggga agaatcgtca
       61 ctattcttct aaattgaaac aagaaatgat ggataaagcc ttactggaag gttgttcaca
      121 aagaagtatt agtcttgatt atgccctccc aaaccaagga atgctttcat tttggccggc
      181 acaatacaag aaaaacgggt atactattgt tgagaaaaca agagggagac cagctaaaat
      241 gggacgtaaa cggaagaaga cttgggaaga aatgacagaa ctagaacgac tccaggagga
      301 gaatgaacgc ttacggactg aggtggccta cctaaaaaag ttaaaagagt tagaggaaag
      361 ggacgaagcc ctagagcgag aaaggcagag acagttagag aaatggtttc aggaggattt
      421 cgactagatt ttcttcttga aacagcacgt ttagctcgct cgacttacta ttatcagttg
      481 aaacaactgg atggggttga taaagataaa gagattaaaa ccgaaattca gggcattgat
      541 aatgaacata aaggcaacta tggctatcgt cgaatccatt tagaactaag gaatcgtggt
      601 tttgtggtca atcataagaa agttcaacgg ttgatgagaa ttcttggttt aacggctcga
      661 attcgccgta aacggaagta ttcttcctac caaggagaga ttggcaagaa agcagagaat
      721 ctcattcaac gccagttcga agcatcgagg ccaatggaaa agtgctatac agatgtgaca
      781 gagtttgcca ttccaaatag cacacagaaa ctctatttat caccagtttt agatggtttt
      841 aacagtgaaa tcattgctta tcatctttct acttcgccaa acttagaaca agtgaagagt
      901 atgttagaac aggcctttac agagaaatac tatgagaata cgattctcca tagtgaccaa
      961 ggctggcaat accaacacga ttcttatcat cggttcctag agagtaagga aattcaagca
     1021 tccatgtcac gcaagggcaa cagcccagac aacggcatga tggaatcttt ctttggcatt
     1081 ttaaaatccg aaatgtttta tggctatgag aagaacttta gatctttaga aaaccttgaa
     1141 caagctattg tggactacat tgattactac aacaacaaac gaattaaggt aaaactaaaa
     1201 ggacttagtt ctgtgcagta cagaactaaa tcctttggat aaattatttg tctaattttt
     1261 tggggtcagt acagtattaa ataccatgat cttatgcgtt ttataataaa aaatctgttc
     1321 ttgttttatt tttagaaaat ttactaaaga ctaaaaatat tatg
//
LOCUS       DBEROU010000100         1194 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.100, whole genome shotgun sequence.
ACCESSION   DBEROU010000100 DBEROU010000000
VERSION     DBEROU010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.100"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(77..568)
                     /locus_tag="ACL8CL_002130"
     CDS             complement(77..568)
                     /locus_tag="ACL8CL_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331286.1"
                     /translation="MKFQTNNSLYKLMNLIIYLFLLYLFIDLLMKSGILDEQIDKLSE
                     LSNLFTIKIETILTVISVVFTFIATLIEYGFSLFLITLFFDKNKRNFGDILLSKNITL
                     SFSILLCYLFNPSDIKFAYFSVLTVLLIGITNYARFKNLRLTVVFSVPFLFNAIMSII
                     NSF"
     gene            complement(549..1145)
                     /locus_tag="ACL8CL_002131"
     CDS             complement(549..1145)
                     /locus_tag="ACL8CL_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HGS1331287.1"
                     /translation="MKIENESKSYDKCILKNISLEIDKGIYRVEGENGSGKSTLLQLL
                     AGLETFDSGLKNCVSKDVLYLDTNQLGVVPFTIEENLQLLCDSFQIHLSFEDKEQINT
                     FFDNKIGDNYMTASTGTKFKLGLSLIFAKNWDYIFIDETLSTVDARSIDMIAKRLISM
                     KESSTIIYVSHNLLNQELISKSQRILIEEGRVYEISNK"
BASE COUNT          470 a          181 c          130 g          413 t
ORIGIN      
        1 aatataagaa ttacaagtta acaaaaccga aatgatttca tatcattatt aagtaaagaa
       61 aaaaatttta gtatttctaa aaactattta taatagacat tatagcatta aataaaaatg
      121 gtacagaaaa cactactgtc aatcttaaat ttttaaatct cgcataattt gttatcccaa
      181 ttagtaatac tgtcaatacg ctgaaatagg caaactttat atccgatgga ttaaataaat
      241 aacaaagtaa aatgctaaaa cttaaggtta tatttttaga taaaagaata tctccaaagt
      301 tcctcttatt cttatcaaaa aataatgtaa tcagaaagag agaaaatcca tattcaataa
      361 gagttgctat aaatgtgaaa acaacagaaa tcactgtcaa tattgtttct attttaattg
      421 taaaaagatt agatagttca gataatttat caatttgttc atctaaaatt cccgacttca
      481 tcagtaaatc aataaacaaa tataaaagaa aaagataaat aatcagattc attaatttgt
      541 ataaactatt atttgtttga aatttcataa acccgtcctt cctctattaa aatcctttga
      601 gatttgctaa tcaattcttg attcaataaa ttatgagata catatataat agtagagctt
      661 tctttcatgg aaattaagcg tttagcaatc atatcaatac ttcttgcatc cactgttgat
      721 aaagtctcat caataaaaat ataatcccaa tttttagcaa aaattaagga taagcctaat
      781 ttgaattttg ttccagtaga tgctgtcata tagttatctc cgattttatt atcaaaaaaa
      841 gtattaattt gttctttatc ctcaaatgat agatggattt gaaaagaatc acacaacaac
      901 tgcaaatttt cttctatagt aaatggaaca accccaagct gattggtatc aaggtatagt
      961 acatctttgg agacacaatt ctttaaacca ctatcaaaag tctctagccc agctaaaagt
     1021 tgtagaaggg tgctctttcc tgaaccattc tctccttcaa ctctgtaaat tcccttatca
     1081 atctctagtg atatattttt caatatacat ttatcataac ttttagattc attttcaatt
     1141 ttcataattt aattccctct atcaacgctg ctaaaattaa taaaagataa gaaa
//
LOCUS       DBEROU010000101         1011 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.101, whole genome shotgun sequence.
ACCESSION   DBEROU010000101 DBEROU010000000
VERSION     DBEROU010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.101"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(41..1011)
                     /gene="ltrA"
                     /locus_tag="ACL8CL_002132"
                     /pseudo
     CDS             complement(41..1011)
                     /gene="ltrA"
                     /locus_tag="ACL8CL_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          270 a          235 c          166 g          340 t
ORIGIN      
        1 tatcctgatc gatttgttct attcttattt cattttacta taattgatag tggtcgcccc
       61 agccagatac cttatctgct atccatttag gaacccctaa cttaagcaat ccccataatc
      121 gtctcgattt cttcttccat tgcttccaga taatcactcg taggcgagta tgcaaacgct
      181 catctatgct ggtgactata cttttcatat ttcccaatga gaaatagttt atccatcctc
      241 gaatagacaa attcagttgc tcaatacgtc ttgttaggtc tatactccat ttcctctgtg
      301 ttagtttctt caatttaagc ttaaatctcc aaacactatc ttgatgtgga cgacttttcc
      361 aaccatctga tgatttccag aacccgaaac ctagatattt caactctctt ggtctggtaa
      421 tcttagtctt ggtcatgttt actttcagac ctagccgttt ctcaataaaa cgactgactg
      481 aatacatcac acgcttagcg gctgcctcgc ttccgaccgt aatcacacaa tcgtctgcgt
      541 agcgaacaaa tcgaagccct ctcttttcta attccttgtc caattcatta agcatgatat
      601 tggataagag aggagataaa tttcctccct gtggcgtgcc aactagcgtt ttataacgtt
      661 gaccgttaat gataacacct gaatgaagat atttacgaat caaggattcc gtatctccgt
      721 cttcgataat gttatgtact aaggacatca atctatcttg aggaactgta tcgaaaaatt
      781 tctctaggtc tatgtccact atccactcat agccgtcatt taagtactct aagagcttca
      841 tgatggcttt ttcacatgac ctatttggtc tgaatccata gctcgtatct gagaaatggg
      901 gttcacaaat agggctaata acttgaacaa tggcctgttg aatcattcta tccataactg
      961 ttggaattcc tagttgacgg atgcctccgt ctggtttagg tatctcaact t
//
LOCUS       DBEROU010000102          877 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.102, whole genome shotgun sequence.
ACCESSION   DBEROU010000102 DBEROU010000000
VERSION     DBEROU010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.102"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            12..877
                     /locus_tag="ACL8CL_002133"
                     /pseudo
     CDS             12..877
                     /locus_tag="ACL8CL_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          263 a          193 c          166 g          255 t
ORIGIN      
        1 gaggtactcc catgctttat gtgggcattg atatcgctaa aaataaacac gatgttacag
       61 ccttgaatgt tccaggaaaa actgttctta aaccactcac tttttcaaat aataaagctg
      121 gttttgaact cttagatctg tctcttcgac agctcaacca agactgtctc atcgctctta
      181 aacttctttc tgaccccaat cgtgaacaat ttcaacacga taatcggcaa gtagaactaa
      241 aaatactggc tagatatatt catcgtctca agaaaaaaca gtctgattgg aaagtacaat
      301 acactcgttg tcttgatatc atctttcctg agttggataa aatcgttgga aagcattcag
      361 aatataccta ccaactcttg acacgctacc ctaatcctca gaaaaggatt gaggcaggat
      421 ttgataagct gatagaaatt aagcattgac cgcttctaaa attcaggata tcctctcagt
      481 cgcacctcgt tctatcggaa caacatctcc tgctcgtgaa ttcgaaatca tcgaaatcat
      541 caaacattac aagagactca ttgacaaggc ggaaacatgt gtcaatgact tgatggctga
      601 gttcaactcg gtcatcacga cggttactgg gattgggaat cgtttagggg cggtcatttt
      661 agccgagatt cgaaatattc atgcctttga taatcctgct caattacaag ctttcgctgg
      721 actggattct tctatttatc agtcaggtca gattgattta gctggaagaa tgatcaaacg
      781 gggttcccct catctgcggt gggcactcat acaagctgcc aaagcatgcg ctcgcttttc
      841 acctgctttt aaggcctatc ttaagactaa gttagaa
//
LOCUS       DBEROU010000103          829 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.103, whole genome shotgun sequence.
ACCESSION   DBEROU010000103 DBEROU010000000
VERSION     DBEROU010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.103"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..399
                     /locus_tag="ACL8CL_002134"
     CDS             <1..399
                     /locus_tag="ACL8CL_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HGS1331288.1"
                     /translation="SEIIAYNLSTSPNLEQVQTMLEQSFTEKHYENTILHSDQGWQYQ
                     HDSYHQFLEGKGIQASISRKGNSPDNGMMESFFGILKSEMFYGYEKTFKSLNRLEQAI
                     VDYIDYYNNKRIKVKLKGLSPVQYRTKSFG"
     gene            complement(578..>829)
                     /locus_tag="ACL8CL_002135"
     CDS             complement(578..>829)
                     /locus_tag="ACL8CL_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HGS1331289.1"
                     /translation="VPAQPGTEDKKLSAPKPDMQPSPQPEGKKPSVPEINQEKEKAKL
                     AVATEKKLPSTGVASNLVLEIIGLLGLIGTSFIAMKRRK"
BASE COUNT          260 a          156 c          144 g          269 t
ORIGIN      
        1 agcgaaatta tcgcctataa tctttcaact tcacccaatt tagaacaagt acaaacaatg
       61 ttggaacagt cattcacaga gaagcactac gagaatacga ttctccatag tgaccaaggc
      121 tggcaatatc aacacgattc ttatcatcag tttcttgagg gtaagggaat tcaagcatcc
      181 atttcacgta agggtaacag cccagacaat ggtatgatgg agtccttttt tgggattctg
      241 aaatcggaaa tgttttatgg ctatgagaaa acatttaaat cacttaatcg attggaacaa
      301 gctattgtag actatattga ttactacaac aataaacgaa ttaaggtaaa actaaaagga
      361 cttagccctg tgcaatacag aactaaatcc ttcggataaa ttaattgtct aacttttgcg
      421 gttcactagc cttctctttt tatatcttag attcatctct atatgaaatg acttataatt
      481 attagatacc aattataagt aaaatctttg ggatcttcaa tggttaacca atcaaatgct
      541 aaatatcgaa agtggatatt tttaaaaaac tgaatcatta ttttcttctt ttcatcgcga
      601 tgaatgaagt tccaatcaaa ccaaggagac caatgatctc aagaactaga ttagacgcca
      661 ctcctgtaga tggcaatttc ttctctgttg ctacagcaag cttagctttt tctttctcct
      721 gattaatctc tggtacactt ggtttcttac cctctggttg agggcttggt tgcatatctg
      781 gttttggagc agatagcttt ttatcctcag taccgggttg tgccggtac
//
LOCUS       DBEROU010000104          780 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.104, whole genome shotgun sequence.
ACCESSION   DBEROU010000104 DBEROU010000000
VERSION     DBEROU010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..780
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.104"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(137..316)
                     /locus_tag="ACL8CL_002136"
     CDS             complement(137..316)
                     /locus_tag="ACL8CL_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331290.1"
                     /translation="MRKVIEELLDSSMSTSAISQGAGVPWTTVSDLRKGKTSMDKMAL
                     LTAEKLYEFATTDKQ"
     gene            complement(458..607)
                     /locus_tag="ACL8CL_002137"
     CDS             complement(458..607)
                     /locus_tag="ACL8CL_002137"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331291.1"
                     /translation="MNKEDWLRLLEKAIDNIPETVTAIASLVTAITVARQNKKRKPKS
                     RQRKR"
BASE COUNT          208 a          149 c          138 g          285 t
ORIGIN      
        1 tagaaaggaa actttctaaa ttgttctttc accgcaggct taggcttgcg gttttttatt
       61 tgctctgatt ctttaaaaaa gcgttttctt gaagaataga gaggtgtttt gtcaaaaata
      121 aaaacagtga ccgaaatcac tgcttatcag ttgtagcaaa ttcatagagc ttttctgccg
      181 ttagaagagc cattttgtcc atgcttgttt ttccttttct gaggtcagaa acagtagtcc
      241 atggaactcc agcaccttgc gaaatagcag atgtagacat cgaactgtct aacaattctt
      301 caataacttt cctcatccta tttgtccttt ttatttttta gataaatata tacattgatt
      361 gcaattataa aaatagctat tgcactaacc attgcatttc ctctttccat ttgataaaat
      421 agaggtgtaa ggggctttcg ccccaacctc ttagcgttta cctttttctt tgacgggatt
      481 tcggtttacg ctttttgttt tgccttgcga ccgttattgc ggtcactaga cttgcgatag
      541 cagttaccgt ttcaggaata ttgtctattg ccttttcaag taacctgagc caatcttctt
      601 tgttcaactt catcaccccc tttccttatc ttgattatat tatatcacgg tacaccgaga
      661 aagtcaagcg ttttgatgaa gtttttttaa ttttttcaaa aaaaaataga ccttgtccag
      721 aggtcgggga gttggagggg gcactctcca aaagcattga tttaataagg ttttatttta
//
LOCUS       DBEROU010000105          605 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.105, whole genome shotgun sequence.
ACCESSION   DBEROU010000105 DBEROU010000000
VERSION     DBEROU010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.105"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(<1..>605)
                     /locus_tag="ACL8CL_002138"
     CDS             complement(<1..>605)
                     /locus_tag="ACL8CL_002138"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HGS1331292.1"
                     /translation="ADALVDADSEADVLAEADALVDADSDALVDAEAEALVDAEAEVL
                     VLAEADALVLAEADALVDAEADALVLAEAEALVDAEAEALVLAEAEALVDADSEALVL
                     AEADALVDADSEADVLAEADALVDAEADALVDAEAEALVDADSDALVDAEADALVLAE
                     ADALVDAEAEALVDADSEALVDAETEALVDAEADALVLAEA"
BASE COUNT          143 a          192 c          141 g          129 t
ORIGIN      
        1 tcagcttcag caagtaccag tgcgtcggct tcagcatcaa cgagtgcttc agtctcagcg
       61 tcaaccagtg cctctgaatc cgcatcaacc agtgcctcgg cttcagcgtc aaccagtgcg
      121 tcggcttcag caagtactag tgcatcggct tcagcatcga caagtgcgtc tgaatctgca
      181 tcaacgagtg cttcggcttc agcgtcaacg agtgcgtctg cctcagcatc aacgagtgcg
      241 tcagcctcag caagcacatc agcttctgaa tctgcatcaa ccagtgcgtc agcctcagca
      301 agtaccagtg cctctgaatc cgcatcaaca agtgcctcgg cttcagcaag taccagtgcc
      361 tcggcttcgg catcaacaag tgcctcagcc tcagcaagta caagtgcgtc agcttccgca
      421 tcaacgagtg cgtccgcttc agcaagtact agtgcatcag cttcagcaag tactagcacc
      481 tcagcctcag cgtcaacaag tgcttcggct tcagcgtcaa cgagtgcgtc tgagtcagca
      541 tcaacgagtg cgtcagcctc agcaagcaca tcagcttctg aatctgcatc aaccagtgcg
      601 tcagc
//
LOCUS       DBEROU010000106          600 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.106, whole genome shotgun sequence.
ACCESSION   DBEROU010000106 DBEROU010000000
VERSION     DBEROU010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.106"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            complement(8..81)
                     /locus_tag="ACL8CL_002139"
     tRNA            complement(8..81)
                     /locus_tag="ACL8CL_002139"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(45..47),aa:Ile,seq:cat)
     gene            complement(95..168)
                     /locus_tag="ACL8CL_002140"
     tRNA            complement(95..168)
                     /locus_tag="ACL8CL_002140"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(132..134),aa:Met,seq:cat)
     gene            complement(174..247)
                     /locus_tag="ACL8CL_002141"
     tRNA            complement(174..247)
                     /locus_tag="ACL8CL_002141"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(211..213),aa:Pro,seq:tgg)
     gene            complement(263..336)
                     /locus_tag="ACL8CL_002142"
     tRNA            complement(263..336)
                     /locus_tag="ACL8CL_002142"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(300..302),aa:Arg,seq:acg)
     gene            complement(346..431)
                     /locus_tag="ACL8CL_002143"
     tRNA            complement(346..431)
                     /locus_tag="ACL8CL_002143"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(395..397),aa:Leu,seq:taa)
     gene            complement(439..510)
                     /locus_tag="ACL8CL_002144"
     tRNA            complement(439..510)
                     /locus_tag="ACL8CL_002144"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(476..478),aa:Gly,seq:gcc)
BASE COUNT          159 a          175 c          137 g          129 t
ORIGIN      
        1 attcaaatgg accttgtagg acttgaacct acgaccactc ggttatgagc cgagagctct
       61 aaccagctga gctaaaggtc cgacaagatc attatagcgg cgaaggggat cgaacccccg
      121 acctcccggg tatgaaccgg acgctctagc cagctgagct acaccgccat gaatcgggaa
      181 gacaggattc gaacctgcga caccttggtc ccaaaccaag tactctacca agctgagcta
      241 cttcccgagt taaatagaaa aatgcaccct agaggagtcg aacctctaac cgcctgattc
      301 gtagtcaggt actctatcca gttgagctaa gggtgctcca tattatgccg aggaccggaa
      361 tcgaaccggt acgatcgtta ccaatcgcag gattttaagt cctgtgcgtc tgccagttcc
      421 gccaccccgg cctctctaag cgaacgacgg gattcgaacc cgcgaccccc accttggcaa
      481 ggtggtgttc taccactgaa ctacgttcgc actgttttct tctatctaaa aatgccggct
      541 acatgacttg aacacgcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
//
LOCUS       DBEROU010000107          510 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.107, whole genome shotgun sequence.
ACCESSION   DBEROU010000107 DBEROU010000000
VERSION     DBEROU010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.107"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>510
                     /locus_tag="ACL8CL_002145"
     CDS             <1..>510
                     /locus_tag="ACL8CL_002145"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HGS1331293.1"
                     /translation="NEMFGENKIFESIDNLFDIIDGDRGKNYPKSDELFSEEYCLFLN
                     TKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRGTVGNVAYYDELIKYKHL
                     RINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQLPITKLKKILLPLPPLA
                     LQNEFADFVA"
BASE COUNT          194 a           76 c           85 g          155 t
ORIGIN      
        1 taacgagatg tttggggaaa ataaaatatt tgaaagcatt gataacttat ttgatattat
       61 agatggtgat aggggcaaaa attatcctaa atcagatgag ttgtttagtg aggagtactg
      121 tttattttta aatacaaaga atgttactaa aaacggattt tcattcgata caaagcaatt
      181 tatcactaaa acaaaggata aattacttcg aaaaggcaaa cttgagcgtt atgatatagt
      241 cttgacaaca agaggtactg ttggaaatgt agcgtactac gatgaattaa taaaatataa
      301 acatttacgt ataaattcag gtatggtaat attacgtccc aagacaccaa atctaaatca
      361 gaaatttatt atccatgttt taaggaataa taattatagt cgagtgatat caggaagtgc
      421 tcagcctcag ttaccaatta caaaattaaa aaaaatactt ctccctctcc cccctctagc
      481 cctccaaaat gagttcgcag actttgtagc
//
LOCUS       DBEROU010000108          480 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.108, whole genome shotgun sequence.
ACCESSION   DBEROU010000108 DBEROU010000000
VERSION     DBEROU010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.108"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..>480
                     /locus_tag="ACL8CL_002146"
     CDS             <1..>480
                     /locus_tag="ACL8CL_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HGS1331294.1"
                     /translation="VKKNHQIPRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLN
                     DFHFEHDFSRLPKIMSWDEYAFTKGKMSFIAQDFDNLNIITVLEGRTQAVIRNHFLRY
                     DRAVRCQVKIITMDMFSPYYDLAKQLFPCAKIVLDRFHIIQHLSRAMSRFRVQIMNQ"
BASE COUNT          141 a           99 c           82 g          158 t
ORIGIN      
        1 tggtaaagaa aaatcaccaa atccctcgta tcatcaacca aaaaattgct caaaagttaa
       61 ttgaaaaaat ttctatgact gatattgccc atcagctggc catttcaact tcaactgtca
      121 ttcgcaagct caatgacttt cactttgagc atgatttttc tcggcttcca aagattatgt
      181 cttgggatga gtatgccttc actaagggaa agatgagttt cattgcgcaa gattttgata
      241 atcttaatat tatcactgtt cttgaaggca gaacacaagc tgtcatccga aatcactttc
      301 ttcgctacga tagagccgtt cgttgtcaag tgaaaatcat tacgatggat atgtttagtc
      361 cttactatga cttggctaaa cagctttttc cgtgtgctaa aatcgttcta gatcgtttcc
      421 atattatcca acatctcagc cgtgccatga gtcgttttcg tgttcaaatt atgaatcagt
//
LOCUS       DBEROU010000109          371 bp    DNA     linear   BCT 24-JAN-2025
DEFINITION  TPA_asm: Streptococcus pneumoniae strain GPSC46 isolate 462686
            SAMN38107084-rid24764143.denovo.109, whole genome shotgun sequence.
ACCESSION   DBEROU010000109 DBEROU010000000
VERSION     DBEROU010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38107084
            Sequence Read Archive: SRR26673285
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales;
            Streptococcaceae; Streptococcus.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JAN-2025) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 18-JAN-2025
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002570513.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002570513.1
            Genome Coverage       :: 181x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 01/18/2025 18:03:05
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,147
            CDSs (total)                      :: 2,115
            Genes (coding)                    :: 1,965
            CDSs (with protein)               :: 1,965
            Genes (RNA)                       :: 32
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1, 1 (16S, 23S)
            tRNAs                             :: 27
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 150
            CDSs (without protein)            :: 150
            Pseudo Genes (ambiguous residues) :: 0 of 150
            Pseudo Genes (frameshifted)       :: 85 of 150
            Pseudo Genes (incomplete)         :: 67 of 150
            Pseudo Genes (internal stop)      :: 49 of 150
            Pseudo Genes (multiple problems)  :: 42 of 150
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38107084-rid24764143.denovo.109"
                     /strain="GPSC46"
                     /isolate="462686"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2017"
                     /collected_by="UKHSA"
     gene            <1..103
                     /locus_tag="ACL8CL_002147"
     CDS             <1..103
                     /locus_tag="ACL8CL_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SapB/AmfS family lantipeptide"
                     /protein_id="HGS1331295.1"
                     /translation="VQVDETDEVDGMRFSTFSTNRCGNWSAFSWENC"
BASE COUNT          109 a           46 c           88 g          128 t
ORIGIN      
        1 tgttcaagtt gacgaaacag atgaagttga tggaatgcga tttagtacat tcagtacaaa
       61 ccgttgcggt aactggagtg cttttagttg ggaaaattgt taattcattt aaattgtaaa
      121 ctttagggat tctctgaagt tatggatgtc gttccaatat gtgtacgttg gaatgttagt
      181 gcttatatgg ggggaacaga atcctctctt gattgaagac aagttagcca ttaggctggt
      241 ttgtctcttt gtcaactgta gtgggttgat ataatagtat tagtgagtgg gataaaagtt
      301 tcatttagtt tattcagtac aaatttaacg ggtcaagatt tattgaactg caccccaata
      361 gttagacaga a
//
